LOCUS       DAOTEX010000001        30904 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3118
            NODE_973_length_30904_cov_4.504781, whole genome shotgun sequence.
ACCESSION   DAOTEX010000001 DAOTEX010000000
VERSION     DAOTEX010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868142
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 30904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 30904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 883
            CDSs (total)                      :: 833
            Genes (coding)                    :: 829
            CDSs (with protein)               :: 829
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30904
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_973_length_30904_cov_4.504781"
                     /isolate="AMR_MDS_3118"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1262
                     /locus_tag="P5056_00005"
     CDS             <1..1262
                     /locus_tag="P5056_00005"
                     /inference="COORDINATES: protein motif:HMM:NF022525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2779 domain-containing protein"
                     /protein_id="HRY62154.1"
                     /translation="YIKDKKPVIFQPVFQKDGFLAAVDILEYDSKHGTYIINEVKSSN
                     EIDEKVHFFDLAFQVNLLRRCGLNIAKINLIHLNKEYVRSGDIDLAGVFKIDNITDEI
                     NELQDQVEGEMAAAKEYLAKETEPNGYCCCITKGRSGHCTTFSHANPNVPEYSVHDIA
                     RIGSSKRKLEELIDSGIFHIKDIPEDMEFSEIQRNQIDSYIHDKVLIKKEEIKKELSG
                     LVFPLYFLDYETYPCAIPRFSGFSPYQQIPFQYSLHVVRAQNEEPEHYEFIHVGKDDP
                     SEHLFKTLSGHIGLVGSVIVWNKKFESKINKEIGLRIPEAASFMENVNSRIYDLMDVF
                     SKQLYVHKDFYGSCSIKCVLPVIVPLLSYKDLEIREGGTASQSWNTITTGDITQKEKD
                     VIVKNLLNYCELDTYAMYAIWNVLRKI"
     regulatory      1307..1413
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            1714..2013
                     /locus_tag="P5056_00010"
     CDS             1714..2013
                     /locus_tag="P5056_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62155.1"
                     /translation="MKKPGSFTLNVYDGKHKKLRIVEISEAEVVNYKTLGIGVGLDVY
                     HCVYMGTRYTKNFLDRVVAYRILDSDDQVKKLEKQTGQVYMRIEFFENKKRKRVR"
     gene            2013..2540
                     /locus_tag="P5056_00015"
     CDS             2013..2540
                     /locus_tag="P5056_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62156.1"
                     /translation="MSKQEKYLKREFVEYYERNGRRWYFYEEGRRPGERLYSVITGPI
                     PLRVIRSGENAIRRYSERMVELRMKSKKKKLAKEHAFALNPNFPISTMCGDVLLKGRI
                     IGAFYNDLVVRLDSPYIGEAGMHYGMFAAMAGIYIFERGKRSVFTKHALETAKNLLVQ
                     IYSQKVSPRKGGNVR"
     gene            2537..2842
                     /locus_tag="P5056_00020"
     CDS             2537..2842
                     /locus_tag="P5056_00020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62157.1"
                     /translation="MKKSDGGSGFNKEVVEAQMSIEILLGEKFSKFGIALFREVDLGT
                     AALILEVDEKIIFDALRTWKLRGRMLKDGPRIEVINLYNWAKDIEMEKWWGSVYKKK"
     gene            2903..3226
                     /locus_tag="P5056_00025"
     CDS             2903..3226
                     /locus_tag="P5056_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62158.1"
                     /translation="MKKEIILILIAVLATIVLGFLILGLPALTPAVEVSEVQTVETYL
                     RENIAEISPVDAVLGGKWYVVSVSVDPMNNSGTVVYEDGHIQEEREFSFVVGENGEIV
                     KLEIR"
     gene            complement(3268..3567)
                     /locus_tag="P5056_00030"
     CDS             complement(3268..3567)
                     /locus_tag="P5056_00030"
                     /inference="COORDINATES: protein motif:HMM:NF024248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRY62159.1"
                     /translation="MDEKIKSELQTYLKTDTFSKSHKLLRVICGPTRHKIIHILNRSK
                     RGLTETEIAHVLSSSLSKISHQMKILKEHELIVSSGKGRDAIYRLANHKIKNFLG"
     gene            3693..4079
                     /locus_tag="P5056_00035"
     CDS             3693..4079
                     /locus_tag="P5056_00035"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY62160.1"
                     /translation="MDEKDKIKKAVLLVEDDSLLLALYSAKLKKDGFEVIPAHTCGEG
                     LAAIKQHEPDLIVLDLKMPDMPGLEFLKILREDPKTQNIKVVIITSSDREEDRICAEK
                     LGVSEYYIKSESGLEQLGNIIKDNAK"
     gene            complement(4135..5103)
                     /locus_tag="P5056_00040"
     CDS             complement(4135..5103)
                     /locus_tag="P5056_00040"
                     /inference="COORDINATES: protein motif:HMM:NF039189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5671 domain-containing protein"
                     /protein_id="HRY62161.1"
                     /translation="MKDEQHKKTTPKDFFLNLGAIVAIYVSVISFINLLLTTLDNLLP
                     DVATGYYNTYESMRWAVAYLVIAFPVFLILSKILNKDIKNSPEKENLGVRKWLTYLTL
                     FVTGIAMTVDLIWLINSFLSGEITLRFSLKALVILVTSLLVFGYYISKVRGLPAQAGE
                     GAKYEKCYKWTSVALVVAAISFGFYVMGSPFTQRLRNIDEKKVGDLQNIQYQIISYWQ
                     QKEFLPEKLSDLEAILGFVVPKDTDKNVDYTYTITGATSFNLCANFNLENEYKNGSPE
                     TSAKYPDVGGKNETWAHQQGDYCYERTIDPTLYSTSPKVTPVKIIQ"
     gene            5152..6465
                     /gene="murC"
                     /locus_tag="P5056_00045"
     CDS             5152..6465
                     /gene="murC"
                     /locus_tag="P5056_00045"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HRY62162.1"
                     /translation="MSIMNLSEIKKAHFVGIGGIGVSAIARLLLLQGKSVTGSDRASS
                     PVTAMLKKLGAKINFGHNENNLEDDTDTVIYTIAVDDTNPEFAKAKKLGLKMFSYPEV
                     LGAISREKYTIAVSGTHGKTTTTAMTGKILIDTDKDPTIIVGSLMKGPMSNLVVGESN
                     LFVVEACEYRRSFLNLSPRIVVITNIDNDHLDYYRDLEDIKSAFAELVGKLGPKDYLV
                     CNKSDERLRGIVANAKCIIVDYSQNKLAEELKLKVVGAHNVENAKAALSVAEILEIDE
                     ASAAKALEGFSGTWRRFEFKGETKGGAMVYDDYAHHPTEIRATLEGARNFFKDKKIFV
                     AFQPHLYSRTKLLLDDFAKSFDDVDVVLVPEIYAAREPVDPSINSEMLVAEIKKNNPG
                     KEIIYMRFLEDIAKYLENNCDANSVIVTMGAGDISGVSDRLISNK"
     gene            6471..7163
                     /gene="rsmI"
                     /locus_tag="P5056_00050"
     CDS             6471..7163
                     /gene="rsmI"
                     /locus_tag="P5056_00050"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015753468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HRY62163.1"
                     /translation="MEPTIYVVATPIGNMKDITLRAIDTLLSADLIVCEDTRVTKKLL
                     QEYEIDKPTISYHLHSKLSKIDEIIAVLKEGKNIALVSDAGTPCISDPGSFLINKARE
                     ELGDELKVVPIPGPSALTTALSASGFTDSSFLFLGFLPHKKGRETLFKEIAASERPVV
                     FYESPHRFMKSLESLQEFLQPGRKIGVARELTKMFEEIKIGTVPEIIDYYTQNADKVR
                     GEFVVMVGSANI"
     gene            7293..7466
                     /locus_tag="P5056_00055"
     CDS             7293..7466
                     /locus_tag="P5056_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62164.1"
                     /translation="MKRLTQKRRMVFLGIGLYMIIAPMYMGIPTATENAIYVLLGAVL
                     LWLSLESEKTTGN"
     gene            7470..8675
                     /locus_tag="P5056_00060"
     CDS             7470..8675
                     /locus_tag="P5056_00060"
                     /inference="COORDINATES: protein motif:HMM:NF024598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HRY62165.1"
                     /translation="MINKKGNTKEGIIIALAIFSIVVAYFLYKKFSFVEPIRYIANDK
                     VQQANVTVTVPVVPAVKERVVTHIKTPEAVKAVYISSWVAGTPSFRNKIIKMVDETEL
                     NAIVIDVKDSTGWISFPMKGAELEKNGSFEKRIADVEDLIDLLHSKNIYVIARIAAFQ
                     DPALVKKRPDLAVLRASTGKPWGDRKGMTWADPGSKEVWDYLIAIGKEAYRVGFDELN
                     FDYIRFPSDGNMNDISYRFNPEKMSKADKIREFFSYLNKNLKPIGAPLSADLFGMTMT
                     NTDDLNIGQVLENALSYFDYVAPMVYPSHYPTGFHNYKNPATVPYEIVKISMDAGVKR
                     AVAASTTPSKLRPWLQDFDMGATYTADMVRAQMKATYDSGLTSWMLWDPSNKYTPTAL
                     ITEGVAKGN"
     gene            8865..9752
                     /locus_tag="P5056_00065"
     CDS             8865..9752
                     /locus_tag="P5056_00065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62166.1"
                     /translation="MSNVSDPVLFMVYLFSLPTIFYCFFCFCRREAALRHFEVELRYS
                     GKPQTDESRIEILNRLISDGAWQDTTNIITLARYRYWLQMKLGKTPRECLSAHSSVYY
                     TCAGAMARKASSINWFFVPSRMFYYTLGAWYLLCAVLISDKLERLTKKTGQQFSANEA
                     DVRVSILSKAGRRREALRIVNDVIGHLLKREDELGYGLNRKEHETLALLWRNAAELED
                     ALGVKLAELSCVNSISYAERSGNPKVLVRILPSAAKIWMKVNKEYAKQLFEWAQQIAE
                     ENGFDDRVKEIRANLEKLK"
     gene            9900..11828
                     /locus_tag="P5056_00070"
     CDS             9900..11828
                     /locus_tag="P5056_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system DNA-binding
                     domain-containing protein"
                     /protein_id="HRY62167.1"
                     /translation="MAENNKDITYFAETDYRNKKTPFGIKTSDRTRHVYVIGKTGMGK
                     STLLENLAVQDIQNGNGMTFIDPHGKSAELLLDYVPKDRIKDVIYFAPHDIDYPVSFN
                     VMEDVGKDQRHLVANGLMASFKKIWPDVWSARMEYISMNIILALLEYPGSTLLGINRM
                     LSDKDYRKKVVDNVTDPSVKSFWVNEFAKYSDKIMVEAGASIQNKFGQFSSNPLIRNI
                     IGQPTSSFNLREVMDNKKIFIANLSKGRMGEINTNLIGGMLITKIYLAAMSRANVSDI
                     DLKKLPNFYVYVDEFQSFVNDSFADILSEARKYKLNLTIAHQYIEQMPETVRNAVFGN
                     VGTTIAFRVGPFDAEVLEKIFAPKFTAEDIVNLGFAQIYLTLMIDGVGSQPFSAKTLP
                     PIALPDIKFTKDIIDNSRAVFAKPRDVVEEEIRKWHEAIPAEGKPAGEKFDKVAVKQE
                     LEFIKKEHGVKEFPPKKTFGEKPAYTSAPMRAPSSPVKPAYNPAPAAAAPAPVRHTPP
                     APVSAPQKPMHTAPQQPVAKSPAKVSLSSLKRAPSDEKKATSENLGDLKNILAKALGS
                     KDKNFNLGKIDAKKDDAAPEHARRVIEEPVKASEPKVSEEIKKEDQSAVPAHAGNAEI
                     KKIKEIPEDVLKKVLEEK"
     gene            11832..12761
                     /locus_tag="P5056_00075"
                     /pseudo
     CDS             11832..12761
                     /locus_tag="P5056_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000744698.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            13355..14557
                     /locus_tag="P5056_00080"
     CDS             13355..14557
                     /locus_tag="P5056_00080"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRY62168.1"
                     /translation="MKKKILVFSLVYYPDVVGGAEVAIKEITDRVSPKDVEFDMVTLR
                     FDKNLPKFEKIGNVNVHRIGFTANSPKIADLVKFPLNLNKYLFPFLSFVKAGQLHRER
                     HYDAIWAMMANYAGFGAMFFKTFHPSVKYLLTLQEGDPIDYIKRRVRFVYPLFVRIFT
                     KADFVQPLSKYLAGFARDMGYKGPMKIVPNAVNTKHFSQDFKAEELNTLKNKLGKKEG
                     DINIITTSRLVLKNAADDVIRSLVFLPDNYNFIILGIGPDMEMLKDLAKKEGVANRVK
                     FLGQVDHKEMPKYLKVSDVFIRPSLSEGFGNSFIEAMAADIPVVATQAGGIPDFLFDP
                     DITPDIPPTGLFCKIRDPKDIARQIKRFMDDVPLREKILKNAKKMVMAKYDWSMIAGD
                     MAEIFKSL"
     gene            14554..16155
                     /locus_tag="P5056_00085"
     CDS             14554..16155
                     /locus_tag="P5056_00085"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRY62169.1"
                     /translation="MIKKVYRKINEKAEEKFPTAYKSLFKNRIFIKYVSAGFVAAFVD
                     LGSLYLFVEYIFYHRYLLAAASAFIFAFVFGAMLQKFWTFQDKNTSGMGFQMVIYIVL
                     AIVNLILNLLIIFALVETLDMWYILAQSISMAILATMNFILYSRFVFKKSLMVPGSVL
                     LAAGIFPPDIGGPATHTLKFMKSFRENGIKTGVITYSDRKIEPEIDDGLDVERVSRKF
                     PRGLRHLIYLAKIFAASLRYETIYAQDISAAGLPAAFVARLVGRRFMVRVGGDLLWER
                     LAEKGKTKRSVINYYNEENYKKRFLYHVGQTVLMSAEKVIVPTDLLANIYRNHYGIVR
                     DNILVLPNPIPAKCETAQLDRKATIGKNILFAGRFIKYKNLDKLIIAFANIYEEIAPA
                     KLILVGDGPEKRDLVRLVKRLNIRNQVIFKAKLPHGNLLEEIRECSVCVAPALTEFNP
                     NFILECLSCGKPVIITKENGLSIKLPERFTWNAMDIKDIENKLTDVVNAGATAGEEIS
                     EIIQKTKTTSWDDIIREHLELFKNS"
     gene            16162..17316
                     /locus_tag="P5056_00090"
     CDS             16162..17316
                     /locus_tag="P5056_00090"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRY62170.1"
                     /translation="MTPNAKDLNVLMLGSDMSILKPGSDAYRRMKEYGSLVNRLDIAI
                     YNGHLRGGNEDNVYQIAPNVFAHPRSFLGLLGLFRVSSRKLSFVLNHRVDLITSQDPF
                     ILGLIGEKLAKWLNTGFQAQIHTDLMSPYFRRESLYNRLKVMIAKSILKRATGVRVVS
                     QKIKDSIRKIRTKAEPVVLPIWTDIEAMKKSPVRINLKEKYPQFKKIFLMASRLTVEK
                     NIFLAVDAMKNILKERHDVGLVIVGEGPYRKLIEGLARGEGLQKNIIFEDWSEDLPSY
                     YKTCDVFLNTSNYEGYGRTIIEALSAGTPVVTTDVGIAKEFVRGGENGFVVPVGSKKE
                     LEKVLKKIVGEDDVLADLKAGTKKTELPLGTKEEYLEAYVKGWVEATSSR"
     gene            complement(17578..18411)
                     /locus_tag="P5056_00095"
     CDS             complement(17578..18411)
                     /locus_tag="P5056_00095"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRY62171.1"
                     /translation="MAETKRPKIGLALGSGSARGLAHIGVIKALEENNIPIDFIAGSS
                     IGALIGGFYASGIDIKEIGEIALNTDWRNLFSMLFEPSFKSGVISGEKVTAFIKKHID
                     EKKFEDCKIPFAAVATDLKTGESVILDKSKMATAIRASISIPLIFKPVEIEGRILADG
                     GLSAPVPVEIVRNMGADIVIAVNLDKHYFDENWSPGWYDIANDSLNILRHHLARADVL
                     DADIVIDIDVGKTYWYQFTNGQDKILAGEKAMKELLPKLQEIIHQKNKKGIRKYLDFF
                     R"
     gene            18674..19291
                     /locus_tag="P5056_00100"
     CDS             18674..19291
                     /locus_tag="P5056_00100"
                     /inference="COORDINATES: protein motif:HMM:NF014045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HRY62172.1"
                     /translation="MAVITKIKETDIYLQIEDQIERRVFVFIDASNLWEAQKAKGKMF
                     DYEKLKRFLTNKFRSPNLKIFYYSAYPAEGTRDYSLDKKHQFFTYLKKGLNFAVIKKE
                     LKRISISNAEGQVVKEKGNMDVEITIDAVHHLNKFDVAVFFSGDSDFLALVTYLRQRN
                     KQVFIFSSKNNISEELRTGGDGYFDVLRLEDDIWGRDLKHRDINT"
     gene            complement(19438..19815)
                     /locus_tag="P5056_00105"
     CDS             complement(19438..19815)
                     /locus_tag="P5056_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62173.1"
                     /translation="MLKNKTIILAIVSFALGALIYNCSKVIFQEGNPWPQIKGIAQLT
                     FGEQNIVKLDIEENKYITKSDSSEIIKSFMKDKGYDFAEQMGSGYLFKSQTGAGAVAT
                     HKYYSRYYSLWTITENSDSEGNL"
     gene            complement(19837..20025)
                     /locus_tag="P5056_00110"
     CDS             complement(19837..20025)
                     /locus_tag="P5056_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011845050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRY62174.1"
                     /translation="MKNEIKKFRTKLGITQEELAEKVGVRRETIVFLEQGKYNPSLKL
                     ALSIARELKMSTDDLFSL"
     gene            complement(20009..20407)
                     /locus_tag="P5056_00115"
     CDS             complement(20009..20407)
                     /locus_tag="P5056_00115"
                     /inference="COORDINATES: protein motif:HMM:NF021463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2178 domain-containing protein"
                     /protein_id="HRY62175.1"
                     /translation="MTLKTYNKIRLGIVFVTAFIFSQSLVIHNFFIPIMVLGLSSLTL
                     FYLRKKVVQIIADERDYQIGGRSALLAIQLTSSVGAIIMFALYALSDTNPLYETIAVT
                     LAFSICILMLTYSAIFKYYSKYDSKDEKRD"
     gene            complement(20873..23266)
                     /locus_tag="P5056_00120"
     CDS             complement(20873..23266)
                     /locus_tag="P5056_00120"
                     /inference="COORDINATES: protein motif:HMM:NF024565.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRY62176.1"
                     /translation="MQVCKINAIKGYKSFADFEWAKFCKYRLVDKKNNVDELKEQVFA
                     KFDVIFGENGSGKSSTCDILKSVSQNQDFQITPPTLAELEICDGANNQTYKYENNSWS
                     GQVDKNSFLFFDVDFINDNIHTHGIRSSNLQQGAHTQKAGKLIIDLDKKANELKIATQ
                     GKKDELEKLQNAFANILAKPFNEKDKEFFQTYKNADNQAKLQKIIGTQAELKKLEEDL
                     ATLQKLNTKYAEINRLSTVGQVVFPNALPTREVVTELFYRQIKEKAQDQADEAIKIHF
                     AKHKRFIESAKSEIPENYTHENCPLCMQPLTNATRVIEYYRAAFDQTYETEKKKFLTD
                     IEMSENELQTIKMNIISLPTKVTAIFDALEKVKTDFAVQDIYKLDEKTEQMGKFSSVS
                     TKDIDDLIIALESLKKIEREQVNGEKLYDAVTLVVQEVEKSIKGVSDLITEKNKLISD
                     FKGKYSDQSKITSEIQEKTQKKMELDRLTDFLRNDVVGQIKNQNEVLEKQKELSEALK
                     KAQEDLRSYLATTIPKNVITRMIEILGKFNLSFTLEHVPGNTQTKEYSFSFKIKDQKD
                     NERDMKKNLSEGERQLISLAFFFAVNENLQNKNTKVLVFDDPITSLDSPNLKILAELI
                     YQKTQEFSQVLVFTHHPLFFKYLTKCGNAAKFGVLKNAEQFGGSFMYFDPGFDLVAEV
                     RQCNQEISQNSQNGTLRPEEIALKYGQLLRLAVERFIKQDLLMWDKEREFEDITRNLK
                     QGKNKMAKLTDEDLETIANIYKYCNYSNLLHADKESPSAFSELTKHIDKFVEILNKV"
     gene            complement(23275..23364)
                     /locus_tag="P5056_00125"
     CDS             complement(23275..23364)
                     /locus_tag="P5056_00125"
                     /inference="COORDINATES: protein motif:HMM:NF013964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HRY62177.1"
                     /translation="MVGLSKGGKTNIKNCEMLCKTHNRAKGNQ"
     gene            complement(23398..24669)
                     /gene="dcm"
                     /locus_tag="P5056_00130"
     CDS             complement(23398..24669)
                     /gene="dcm"
                     /locus_tag="P5056_00130"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010519511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
                     /protein_id="HRY62178.1"
                     /translation="MKRIRVAELFAGVGGFRLGLESASDKNSKYEVVWSSQWEPGAKT
                     QHASEIYTYNFGEEGHSNEDIQEVIEKKFNEIRDHDLLVGGFPCQDYSVARVASQAKG
                     LNGKKGVLWWSIYKILERKGKNAPSFLMLENVDRLLKSPSAQRGRDFAIMLSSLNNLG
                     YIVEWRVINAAEYGMPQRRRRVYILGYKKGTGIYKKVKAEKNIDRWVDSDGVIAKAFP
                     IKKTTGLCDQFNLEKDLAKLSKNFPLPKPTKSPFEIAGVMIDGKVYTKKVTPDHRKEF
                     AILKDIIESDEKNIPKEFFIDMKDMPRWEYLKGGKTEERKTKSGHIYNYSEGSMVFPD
                     PLNKPSRTIVTGEGGSSPSRFKHVIKTKSGKLRRLTPEELEKLNMFPAGHTKFMKNGK
                     ETPATKRAFIMGNALVVDVIKKLGSSLLDYL"
     gene            complement(24719..26104)
                     /locus_tag="P5056_00135"
     CDS             complement(24719..26104)
                     /locus_tag="P5056_00135"
                     /inference="COORDINATES: protein motif:HMM:NF040973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sau3AI family type II restriction endonuclease"
                     /protein_id="HRY62179.1"
                     /translation="MNTTDYKTKEEVLKQAQIILNKSLREIIPKEDVVKVENDILNYK
                     NRRKGLLGELIEWYVFGKKPDGKSEADFSIAGVELKTTPLKKSPKKQFVSKERLVFSM
                     IDYDKIVHEKWESSSFLKKNKLVLLMFYLWIQSQSIIDHEFKFTYLLNLLEDISKEDI
                     FQIQKDWEYIVAKISRGEAHLLSEGDTYYLGACTKAANSRVVRDQPRNRIPAKPRAFS
                     LKQQYLNYLIQKEILGKKVDTKSIFKKQRRIETIEDAIKEIFSPYVGKTDTQIISSLG
                     LSLNKRAKGYKRLLINKIIGIDGGKIEELEKANVTLKVITLEHTGTLRESISFPAFDY
                     NDLITQVWYDDKEETMSDFHAQLETKKFLFVIFQKIKNSDEIILKKTMFWNFPMKDLP
                     EAERVWQETIDCINEGKYKDLPKITGSSVAHVRPHGKNAEDTIKTPQGTMETKRCFWL
                     NAKYIQQAIEN"
     gene            complement(26109..26765)
                     /gene="radC"
                     /locus_tag="P5056_00140"
     CDS             complement(26109..26765)
                     /gene="radC"
                     /locus_tag="P5056_00140"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HRY62180.1"
                     /translation="MKIKDLPKIDRPREKLEKYGPEKLLDHELLAILLGSGIKGLSVL
                     ELSKKILSTISKVGIDKVTLKDLLEIKGLGKAKASEIISCFELGRRVLKDKKTSILLS
                     PKDVWERMPDIRASKKEHFVVFYLDSRNQEIRREIISIGTLNESLVHPREVFESAIKN
                     NAASIMLAHNHPSGNTESSQDDIEITKKLVHAGKILDIHIVDHVIVTEKMFSSIIDLI
                     "
     gene            complement(26916..27215)
                     /locus_tag="P5056_00145"
     CDS             complement(26916..27215)
                     /locus_tag="P5056_00145"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRY62181.1"
                     /translation="MQNKNIKNINKIKSFDKLFKELSKSKNFVNSYNEELFRLKMASR
                     IKTLRTQGRMTQKGFADKARMPQSVIARIESGNHTFSLVTLNKVAHALGKKVELV"
     gene            complement(27244..27558)
                     /locus_tag="P5056_00150"
     CDS             complement(27244..27558)
                     /locus_tag="P5056_00150"
                     /inference="COORDINATES: protein motif:HMM:NF017757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRY62182.1"
                     /translation="MQTYKLAFLGGAECFIKTLPHDAPAKIMATIKMMSMDMSVVNIR
                     KLGRAIHELKINKYRILFFIDHDTICFVSGFIKKSAKTPVREIRNAETIFKKYINTKT
                     KK"
     gene            complement(27953..29245)
                     /locus_tag="P5056_00155"
     CDS             complement(27953..29245)
                     /locus_tag="P5056_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62183.1"
                     /translation="MKKIFLISIGLISAIVVVMFFAQICPPKGPWPMPPWCSSEYKIY
                     EYLVDTKPTRISQIKAINMYDTWGRNYNMGMVENTQANIESSFDRIKSLGAEEVYVHD
                     FDRAVYGDEQDFSSTDYTIEDDVFSNDMRDESINENDLKKLVTAAHERGLKLGIKRNL
                     TFIDMKNLIISGIRGTISADVSKNFSEFNENHSEEWINDYFQKWENRLVEKAKLYSRA
                     GVDIMSISPTFQDPQFFGHEELADKLWSDLIDEVKNNFSGKILAEINIYGLVDGNNGK
                     ENWNKYDYYKKADITEARIYKILEKYQSPDKNQIKQDMSKMVSDLDRKAQKLGIKLSI
                     FFAPSSYKNGLYNNPVEFLDINNQEIKNIEKDYESQSEAYNYLFESLNKTNNISRINA
                     ANFTWDDALDPEVRPRISISASFRNKPAEQIIKAWYNK"
     gene            complement(29559..29645)
                     /locus_tag="P5056_00160"
     tRNA            complement(29559..29645)
                     /locus_tag="P5056_00160"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(29609..29611),aa:Ser,seq:gga)
     gene            29738..30244
                     /locus_tag="P5056_00165"
     CDS             29738..30244
                     /locus_tag="P5056_00165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62184.1"
                     /translation="MKKIVVNDIMQKGYLYELTEPVGKNFAKDFKPQLTPKEMLALGV
                     FGGRYMTDCKKEFPADWYKEAKLCHERHDPELNYFKVNASQPLSAWRAKGWINEKHDP
                     RGWFQWYCRYYMGRRLPEEDEKQIKRWRAIARHIAQVKKNCRAGDLNCRRRQRQSLLH
                     WAIDSRKL"
     gene            30696..>30904
                     /locus_tag="P5056_00170"
     CDS             30696..>30904
                     /locus_tag="P5056_00170"
                     /inference="COORDINATES: protein motif:HMM:NF015377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
                     /protein_id="HRY62185.1"
                     /translation="MQTRAIKLDIPFVKQINDYFCGPAALQMVFGYFNCEKPQVELAE
                     IMNTKENVGTKKKKMVEAVLEHDFH"
BASE COUNT         8888 a         6138 c         6656 g         9222 t
ORIGIN      
        1 gatatattaa agacaaaaag cctgttatct ttcagcctgt atttcagaaa gacgggttct
       61 tggcggcggt ggatattctt gaatacgatt ctaagcacgg cacatacata atcaatgaag
      121 ttaagtcttc gaacgagata gacgagaaag tgcacttttt tgatctcgct tttcaggtaa
      181 atcttctaag gaggtgtggg ttaaacatcg cgaaaataaa tctcatacat ttaaataaag
      241 aatatgtgcg ttcgggcgat atagatctag caggtgtatt taagattgat aatattacgg
      301 atgagataaa tgaattacag gatcaggtag aaggggagat ggctgccgcg aaagaatatc
      361 tcgcaaaaga gaccgagccg aacggatatt gctgttgcat tacgaaaggg cgaagcggac
      421 actgcacaac cttttctcat gcaaacccaa acgtccctga atatagtgtc catgacattg
      481 cgaggatagg tagtagtaaa agaaaattag aagagctcat agacagcggt atttttcaca
      541 tcaaagatat tcccgaggat atggagtttt cggaaataca gcgaaatcag atagactcat
      601 acattcacga caaagtcctc ataaaaaagg aggaaataaa gaaagagctt tcggggctcg
      661 tttttcctct atattttttg gattacgaga catatccgtg cgcgattccg cgattcagcg
      721 gcttttctcc ttatcagcag attccttttc aatattctct gcatgtagtc cgcgcgcaaa
      781 atgaagagcc ggagcactat gaattcatac atgtcggaaa agatgacccg agcgagcatc
      841 ttttcaagac tctttcagga cacataggtc tcgttggttc ggtcattgtt tggaataaga
      901 aatttgaatc aaagataaac aaagagattg gattaaggat acccgaagcg gcctctttta
      961 tggaaaatgt aaattctcga atatacgatc tgatggatgt attttcaaaa cagctttacg
     1021 ttcacaaaga tttttatggt agctgttcaa tcaagtgtgt gttgcccgtt attgtccccc
     1081 tgctttcata taaagatctg gagataagag aaggcggcac cgcttcgcaa agctggaata
     1141 caataacgac aggggatatt acacaaaaag aaaaggacgt aatagtaaaa aatcttctca
     1201 attattgtga actggataca tacgctatgt atgcgatttg gaacgttctt agaaaaatat
     1261 aatttgcaaa aaaattacaa ggtgtaaaat atacttacaa ttaaataact tatcaagagt
     1321 gcggggaggg tactagccca ttgaaccgcc ggcaaccacg gggagtttat ccttgaacgg
     1381 tgccaaatct agcccgaaag ggaaagataa tcgtcgtaac tctttctgtt tcggaagggt
     1441 ttttctttgc cccgtaggaa gttgcacgtg gggggttatt ataagagaac acggagccgc
     1501 caggcggatt ccgagagtag aaagaaaaac aaggtattta tttgatacga actttccttc
     1561 tggggtttgt ctttataaaa aaaattaaat ctggattgcc gcacgcttcg cgttcgcaat
     1621 gacataaaaa caggagagga ggttagaagg gcacaaaacg atagcgggcg aaagcccccg
     1681 agtcgccgat gtaaatcgga gaaggagtaa agtatgaaaa agccgggttc tttcacgctc
     1741 aatgtttacg atggcaagca caagaaatta cgcatcgttg agatctctga agctgaagtg
     1801 gtgaattata aaacgctagg tattggtgtg gggcttgatg tgtatcattg tgtttatatg
     1861 ggcacaaggt ataccaaaaa tttcctggac cgcgttgttg cctatcggat tctagatagc
     1921 gacgaccaag ttaagaaact tgagaagcaa accgggcaag tttatatgag aattgagttc
     1981 tttgaaaata aaaaaagaaa gagggtgcga taatgagtaa acaggaaaaa tacttaaaga
     2041 gggagtttgt cgagtattat gagcggaatg ggaggcgttg gtatttttat gaagaaggtc
     2101 ggcgtcccgg agagagactg tattctgtaa tcacgggacc gataccactt cgagttatca
     2161 ggtctggaga aaatgcgatt agaagatatt ccgaaagaat ggtagagctg aggatgaaat
     2221 caaaaaagaa gaagcttgcg aaggagcatg cctttgcgct taacccaaat ttccccatat
     2281 ctactatgtg tggtgatgtc ttgttaaagg ggaggattat cggagcattt tacaatgatc
     2341 tcgtcgtaag acttgatagt ccttacattg gcgaagcagg catgcactac ggaatgtttg
     2401 ccgctatggc cggaatctat atctttgaga gaggtaagag aagtgtgttt acaaaacacg
     2461 ctctcgaaac cgcgaaaaat ctattggttc agatttattc acaaaaggta tcacctagga
     2521 agggcggtaa cgtgagatga agaaaagtga cgggggctct ggcttcaaca aagaagtggt
     2581 tgaagctcaa atgtcgatcg agatactgct cggcgagaaa ttctcaaagt ttggcatcgc
     2641 actctttcgg gaagttgacc ttgggaccgc cgctttgatc ttggaagtgg atgaaaagat
     2701 tatttttgac gctttgagga cttggaagct aaggggcaga atgttgaaag atggacctcg
     2761 tatcgaagtc atcaatcttt ataactgggc gaaagatatc gaaatggaga agtggtgggg
     2821 gtcagtctat aaaaagaaat agtacaaagt agttctttat aacccgccat tcggcgggtt
     2881 ttatttatgt taaaatagac atatgaagaa agaaattatt ttaattttga ttgcggttct
     2941 agccacaata gttctcgggt ttcttatctt agggctacct gcgctcactc ccgctgtaga
     3001 ggtaagtgag gtgcagacag ttgaaacata tttaagagaa aatattgcag aaatttctcc
     3061 cgtggacgct gtgcttggcg gaaaatggta tgttgtctct gtctccgttg acccgatgaa
     3121 caattccggc acggtcgttt atgaagatgg ccatatacag gaagaaagag agttttcttt
     3181 tgttgtaggg gaaaatgggg agatagtaaa attggagata agataatgat ttaaaacaaa
     3241 aaagctccgc atgggagctt ttttgtttta gcctagaaag tttttaatct tgtggtttgc
     3301 aaggcgatag atggcgtctc ttcctttgcc gcttgaaaca atcagctcat gctcttttaa
     3361 tatcttcatt tgatgagata tttttgaaag agaggaacta agtacgtgtg caatctctgt
     3421 ttcggttaac cctcttttgg acctgttgag aatgtggatg attttgtgtc tggtcggacc
     3481 gcagattacg cgcagtaact tgtgggattt gctgaaggtg tctgttttca aatatgtttg
     3541 cagttcgctt tttatttttt cgtccatgtt gtttattgat tagtgatatt aaattaatta
     3601 tacaacatta ttcggatttg ggtatttgtg gacatcattt gtgtgggata taattattta
     3661 tgttgaataa attttcaata tataaataaa aaatggacga aaaagataaa ataaagaagg
     3721 cagtgttgct tgtagaagat gacagtcttc tcttggctct ttattccgcg aagttaaaaa
     3781 aagacggctt tgaagtaatt cccgcgcata cttgcggaga aggccttgct gcaatcaaac
     3841 agcacgagcc ggatcttatc gttcttgatt taaaaatgcc ggatatgccg ggactggagt
     3901 ttttgaaaat acttagagaa gacccaaaga cacagaacat taaagttgtt attataacct
     3961 caagcgatag ggaagaggat cggatttgcg cggaaaaatt gggggtgtct gagtattata
     4021 taaagtcaga gtccggtttg gaacagttgg ggaatataat caaagacaac gccaagtaga
     4081 tatttcttta agcaaaaata aaagccccga tgtggggctt ttttgttttg ctttttactg
     4141 aattattttt acaggcgtaa cttttggaga tgtggagtat agagtcgggt caattgttct
     4201 ctcgtagcaa taatctcctt gttggtgggc ccatgtctcg tttttgcctc ccacatccgg
     4261 atacttagcg gatgtttcag ggctgccgtt tttgtattca ttttccagat taaagtttgc
     4321 acaaagattg aatgacgtag caccggtgat tgtgtaggta tagtccacgt tcttgtctgt
     4381 atcttttgga acgacgaacc ctaagatggc ttcaagatcg gaaagttttt ccggtaaaaa
     4441 ttctttttgc tgccagtagc ttattatctg atactggatg ttctgcagat cgcccacttt
     4501 cttttcgtct atatttctaa gcctttgtgt aaaaggagat cccattacat aaaatccgaa
     4561 agaaatcgct gcgacaacca gtgcgacaga tgtccatttg taacattttt cgtatttggc
     4621 tccctctcct gcctgcgcag gcaggccgcg aacttttgaa atgtagtagc caaaaacaag
     4681 aagtgatgtt acaagaatta cgagagcctt aagagagaat ctgagcgtta tctcgccgga
     4741 caggaagctg tttatgagcc agattaggtc cacggtcatt gcaatcccgg ttacgaagag
     4801 ggtgagatat gtgagccact ttctgactcc aagattttct ttctcgggac tattttttat
     4861 atctttgttc aggattttgg agagaatcaa aaataccggg aaggcgatca caagatacgc
     4921 cacagcccat cgcattgatt cgtaagtatt gtaatagccg gtagctacgt ccggcaggag
     4981 attgtccagt gtcgttaaaa gcagattgat aaagctgatg acgctcacat atattgcgac
     5041 tatcgcccca aggtttagga aaaaatcttt tggcgtagtt tttttgtgtt gttcgtcttt
     5101 catgattttt tattgtatat ttattattaa taaggtaatt atatcacaaa aatgagcatt
     5161 atgaacttgt ctgaaataaa gaaagcgcat ttcgttggga taggcggtat aggcgtgtct
     5221 gctatcgcaa gactcctgct tcttcaaggt aagtcagtta ccggctcaga cagggcatct
     5281 tctcccgtga ccgcaatgct taagaagctc ggtgctaaaa taaattttgg tcataatgaa
     5341 aataatttag aagatgacac tgatactgtt atctatacaa ttgcagtaga tgatacgaac
     5401 cccgaatttg ccaaagcaaa aaagcttgga ctaaaaatgt tctcgtatcc ggaagttctc
     5461 ggtgcgatat ctcgcgagaa atacacgatt gctgtctcgg gaacgcacgg caagaccacg
     5521 acgaccgcga tgacggggaa aattttaatc gatacagaca aagacccgac gattattgtg
     5581 ggtagtctta tgaaggggcc catgagcaat ttggtggtgg gcgagagtaa tctttttgtt
     5641 gtagaggctt gcgaatacag aaggtctttt cttaatttgt ccccgagaat tgttgtcatc
     5701 acaaatatag acaatgatca tctagattat tatcgcgatt tggaagatat aaagagcgct
     5761 tttgcggagc tggtgggaaa gctcgggcca aaagactatt tggtttgtaa caagagtgat
     5821 gagagacttc ggggcattgt ggcgaacgca aaatgcatca ttgttgatta ttcccaaaat
     5881 aaattagccg aagagttaaa attaaaagtt gtgggcgcgc ataatgtgga gaacgcgaaa
     5941 gccgccctgt ctgtggcgga aatattagag atagacgagg cgtccgcggc caaggcgctt
     6001 gaaggttttt ccggcacctg gcgcagattt gaatttaaag gggagacgaa gggcggcgcg
     6061 atggtatatg atgactacgc tcatcatcct acagagatac gggccacgct agagggagca
     6121 aggaactttt ttaaggacaa aaaaatattt gtagcattcc agccgcacct ttatagcaga
     6181 acaaaattgc ttctcgatga ttttgcaaaa agttttgatg acgtggatgt ggttcttgtg
     6241 cccgagatat atgcggccag agagcctgtc gacccaagca tcaattcaga gatgcttgtc
     6301 gccgaaataa agaaaaacaa tccaggaaaa gaaataattt acatgagatt tttggaagat
     6361 attgcaaaat accttgaaaa taattgcgac gccaattccg taatcgtcac aatgggggcg
     6421 ggtgacattt cgggcgtatc tgatagattg atatctaata aataaaatat atggaaccga
     6481 ctatttatgt agtagccacg ccgataggca atatgaaaga tattacgctc cgtgcgatag
     6541 acacgcttct ttctgccgac cttatcgtct gcgaagacac gcgcgttacg aaaaagcttt
     6601 tgcaggaata cgagatagac aagccgacaa tctcttatca tcttcacagt aagctatcaa
     6661 agatagacga gataatcgca gtattgaaag agggcaaaaa tatcgctctt gtgtccgacg
     6721 ccggcacgcc ttgcatctcc gatcccggaa gcttcctgat aaacaaagcg agggaggagc
     6781 ttggcgacga gctgaaagtt gtcccgatcc cgggtccatc cgcgcttact acggcacttt
     6841 ccgcttcagg gttcacggac tcatcttttc ttttccttgg ttttcttcct cacaagaaag
     6901 gccgcgagac gctcttcaag gagatagccg cgagcgagag gccggtagtc ttttatgaat
     6961 ctccacacag atttatgaag tcgcttgagt cgcttcagga atttttgcag ccggggagaa
     7021 agatcggcgt tgcgagagag ctgacgaaaa tgtttgaaga aataaagata gggaccgttc
     7081 cggaaattat tgactactat acgcaaaacg cggacaaagt gagaggggag tttgtggtga
     7141 tggttggatc tgctaatata tagatatgat cccattagaa gccgcgatcg cagcctacga
     7201 atgacacggt gtggttactt tacgggaatt aagaatttaa taacttaata aacagcagag
     7261 agcgcgttag accgcgacct gcttctaacg ggatgaaaag attaactcaa aaaagaagaa
     7321 tggtgttttt ggggataggg ctttatatga taatcgcccc gatgtatatg ggcattccta
     7381 ccgcgactga gaatgcgata tatgttcttt tgggggcagt gttgctttgg ctttcgttag
     7441 agtctgaaaa gacgacggga aattaaaaaa tgataaacaa gaaaggaaac acaaaagaag
     7501 gaatcatcat cgccctcgcg attttttcta ttgttgtcgc atattttctg tataaaaaat
     7561 tttcttttgt tgaaccgata agatatatcg cgaatgacaa agtccagcag gcgaatgtca
     7621 ccgtgaccgt gcctgtggtg ccggccgtga aagagcgcgt ggtgactcac ataaaaactc
     7681 ccgaggctgt gaaggcggta tatatctcta gctgggtagc gggcacgccg tcttttcgaa
     7741 acaagattat aaagatggtg gacgagaccg agctaaatgc aatcgtgata gacgtgaaag
     7801 attccaccgg atggatctct ttcccgatga aaggcgcgga gctcgagaag aacggatcat
     7861 ttgaaaaaag aattgccgac gttgaagacc ttattgatct gcttcactca aaaaatattt
     7921 atgttatcgc aaggatcgcc gcgtttcagg atcccgctct cgtgaagaag cgaccggact
     7981 tggcggttct tcgtgcgagc acaggcaagc cgtggggtga taggaagggg atgacctggg
     8041 ctgatccggg atcaaaagaa gtctgggact atttgatagc cattggcaag gaggcctata
     8101 gggttggttt tgacgagctt aactttgact acatcaggtt cccttcagac ggaaatatga
     8161 acgatataag ctacagattt aatccggaaa aaatgagcaa ggccgataag atcagagaat
     8221 tcttttcata tttaaacaag aatttgaaac ctatcggtgc gcctctctcg gcagaccttt
     8281 tcggaatgac gatgacaaac acagacgatc ttaatatcgg tcaggtttta gaaaacgcct
     8341 tatcttattt tgactatgtt gctccgatgg tatatccgtc acattacccg accggttttc
     8401 ataattacaa aaatcccgct actgtgcctt atgagattgt aaaaatatct atggatgccg
     8461 gcgtaaaaag agcggtagcc gcaagcacaa ctccgagcaa gctccgacct tggcttcagg
     8521 attttgatat gggcgcgaca tacacagcag atatggtgcg cgcacagatg aaggcgactt
     8581 acgactccgg cttaacttcc tggatgctct gggacccttc aaacaaatat actccgacag
     8641 cgcttattac cgagggagtc gcgaaaggca attagtaaaa aataaaaaat ataagaaaaa
     8701 acggctaacc tgggtggtta gccgttcatc tgtcaagtat atattgacaa taaccaataa
     8761 aagtgtataa taaattggaa aatagtaaat tgataaatat atagtaagta ggcctgcaaa
     8821 cgatatcatc tgaaatccgc gttgctgaaa agggaggaat tggaatgtct aatgtaagcg
     8881 accccgtcct gtttatggtg tatctcttct cgcttccgac catcttttac tgcttctttt
     8941 gtttttgccg tcgcgaggcg gcgttgaggc attttgaggt cgaactacgt tacagcggca
     9001 aaccacagac ggatgagtcg cgtattgaga ttctgaatcg cctcatttct gacggagcgt
     9061 ggcaagatac cacaaacatc atcacgcttg ccagatatcg ctactggctc cagatgaagc
     9121 tcggcaagac cccccgcgag tgcttgtcgg cccattcgtc cgtgtactac acgtgtgcag
     9181 gggccatggc gcgtaaagct tccagtatta attggttttt cgttccttcc cggatgtttt
     9241 attacaccct tggtgcatgg tatcttcttt gcgcagtgct catctccgac aaactggaga
     9301 gacttacgaa gaagacgggc cagcagttct cggcgaatga ggcggacgtc agagtctcta
     9361 tcctctcgaa agcggggcgg cgtagggagg cgcttcggat tgtcaacgat gtgataggcc
     9421 atcttctgaa aagagaggat gaactcggtt atggcctaaa tcgcaaagag cacgagacgc
     9481 tcgcgctatt gtggaggaac gcggccgaac tagaggacgc gcttggggta aaattagccg
     9541 aactttcttg cgtcaactcc atctcctacg ccgagaggtc agggaatccg aaggttctcg
     9601 tccgcatact tccgtccgcc gccaagatct ggatgaaggt gaacaaagaa tacgctaagc
     9661 aacttttcga gtgggcgcaa cagattgccg aagaaaatgg cttcgatgat cgtgtgaagg
     9721 agattcgtgc gaatctcgag aaactgaagt aggggactct cctccctcgt ggcggttgtc
     9781 agaaacgacg accgcctttt ttgtttggaa tttaagccgt tttatcttat aattgaaaga
     9841 cttgaagatt ttataaaaac attcttaatt ctttattcat aattcctaat tcttaaacta
     9901 tggctgaaaa taacaaagat ataacatatt ttgcggaaac agattataga aacaaaaaga
     9961 ccccttttgg aataaagacc tccgatagga caaggcacgt ttatgtcatc ggaaaaacgg
    10021 ggatgggaaa atcgaccctt ctcgagaact tggctgttca ggacatccaa aacggcaacg
    10081 gtatgacatt catagatccg cacgggaagt ctgcagagct tcttcttgat tatgttccga
    10141 aggacagaat taaagacgtt atatattttg ccccgcacga tattgattat ccggtctcat
    10201 tcaacgtgat ggaagacgtc ggcaaggatc agaggcacct cgtcgcgaac ggactcatgg
    10261 cctcgttcaa aaagatttgg ccggatgtct ggtctgcgag aatggaatat atttccatga
    10321 acattatttt ggcgctcctc gaatatcccg gctcgaccct ccttggtatt aacagaatgc
    10381 tttccgacaa ggattacagg aagaaggtcg tggacaacgt cacagaccct tctgtaaaat
    10441 ctttctgggt gaatgaattc gcgaagtata gcgacaagat catggtggag gccggagctt
    10501 ctatccagaa caagttcggt cagttttctt ccaacccgct catcagaaac atcatcggcc
    10561 agccgacgtc atctttcaat ttgagagaag tgatggacaa caaaaagatc ttcatcgcga
    10621 acctttcaaa aggaagaatg ggagagatca acacaaacct gattggagga atgcttatca
    10681 caaagatcta tcttgcggca atgagcagag cgaacgtttc tgatatcgac ctgaagaagc
    10741 ttccaaactt ctatgtatat gtagatgagt tccagtcgtt cgtgaacgat tcttttgccg
    10801 acattctttc cgaggccaga aagtataaat taaatttaac aatcgctcac cagtatatcg
    10861 agcagatgcc ggagactgtg cgaaacgccg tattcggcaa cgtgggcacg accatcgcat
    10921 tccgtgtggg gccttttgac gccgaagtgc ttgagaaaat atttgctccg aagttcactg
    10981 ccgaagatat tgtgaatctc ggtttcgccc agatatatct cacgcttatg atagacggag
    11041 tcggttctca gccattttcc gcgaagactc tcccgcctat tgctcttccc gatatcaaat
    11101 ttacaaaaga tatcatcgat aattcgaggg ccgtattcgc gaagcctcgc gacgttgtcg
    11161 aagaagagat cagaaagtgg cacgaagcta tacctgcgga gggcaagcct gcgggagaaa
    11221 aattcgacaa agtcgccgtt aagcaagaac ttgaattcat aaagaaagag cacggagtaa
    11281 aagaatttcc tccaaagaaa acatttggag agaagccggc ttacacctcg gctcctatga
    11341 gggctccttc atctcctgtg aagcctgcat ataatcccgc tccggctgca gccgccccag
    11401 ctcccgtaag acacacaccg ccggcacctg taagcgcgcc tcaaaagcct atgcacacag
    11461 caccacagca acccgtcgcc aagtcacctg cgaaggtctc tttgagttct ctaaagaggg
    11521 ctccttctga cgaaaagaag gctacaagcg agaatttggg cgacctgaag aatattttgg
    11581 ccaaagcgct tggttccaaa gacaagaact tcaatctcgg taaaatagat gccaagaaag
    11641 atgacgccgc tcccgagcac gcgaggcgag ttatcgaaga gcctgtgaaa gcaagcgagc
    11701 cgaaagtttc agaagaaatt aaaaaagagg atcagtctgc cgtgcctgcg cacgcaggga
    11761 atgcagaaat taaaaaaata aaagaaattc cggaagacgt tctaaagaag gtcctggaag
    11821 aaaaataata aatgaaaaag acgatactca tgaccggtgg cgccggattt cttggttcac
    11881 acttgtgcaa gaaatatctc gatgagggac atcgagttat ttgcgtggac aaccttcaga
    11941 caacgatcgt tccgaagaac attgagccgt tcctaaaaaa taaaaatttc aaattcataa
    12001 agcacgatat cgttaagcct ctcgacatcg gcgaaaagat agattggata ttcaatctcg
    12061 cctgttccgg ctcatacacg atatatcaat acaatcccgt tcatacgatg gagacgaaca
    12121 ccacaggaat gataaacatg ctaaatctcg cgaggagaaa cggcgcaaga attatgcagt
    12181 tctctacttc agagatctat ggcgacccgc tggaatcacc gcagagagaa gactacagag
    12241 gcaacgtgaa ctcactcggt cctcgcgctt gttacgatga gggcaagaga agtgccgaga
    12301 cactttgcat ggattattac agagagttcg gcacagacgt gaagatcatc aggatcttca
    12361 acacatacgg tccgaatatg gatatgaacg acggacgagc catgagcaac ttcattgcga
    12421 acgctctggc cggaaaagat ctcgttatat acggagacgg cacgaacacc agaagcttcc
    12481 agtatgtaga tgacctcgtc gagggaatag ataaaatgat ggtgaaggac ggatttatcg
    12541 ggcctgtgaa tttgggcaat ccgggagagg tgacgatgaa agacttggcc gagatgatcg
    12601 tcggcaaagt cggctcaagc tcaaagataa tttacgaagc cgccgcttca gacgatccga
    12661 agagacgaag cccggacatt tctctcgcgg ggaaagagct cggttgggcg ccgaagatct
    12721 cactttccga gggactcgat aagacgatcg catatttcaa aacaatccca cagcccgaga
    12781 gcaggatctt ggtattttct actacatact caccggacct cggcccggcc gagcaggcca
    12841 ttttcgatct ttcaaaagag atgaaagaca ccgagttcca tgtcatcacg accaagttca
    12901 ggagcggtat cccttctctc gaaaagataa acaataattt tatttacagg atcggtttcg
    12961 gtaacaagtt tgataaatac ctgcttcctg tcgtcgctcc gttcaaggct tactctttga
    13021 acaaaaaata cggcttcaag ttcgcgtggt ccatcatggc gagctatgca ggcctttccg
    13081 cccttatctc aaaaatactc ttgggtaaga taagcttcct cattacgctt tcagagggcg
    13141 aagtttcttc cccaatatcc agacttatct caaaatcagc cgattcggta ttcgtctcaa
    13201 acctcgaaga ggaaagagaa gcaaagatcg tctctccggg aatgaaagtt tctacccgag
    13261 caactgacgg tagggctttt gtagatcagg tcagatacca ctacgcagac cttttgaata
    13321 agcaaggaag taggctcgca aggccaaaat agaaatgaaa aagaagatct tagttttttc
    13381 acttgtttat taccccgacg tggtcggtgg agcggaagtt gctataaaag agataaccga
    13441 tagggtctct ccgaaggatg ttgaatttga tatggtgacg ctccgttttg ataaaaatct
    13501 tcccaaattt gaaaagattg ggaatgtgaa tgtccacagg attggtttta ccgcaaattc
    13561 accgaagatc gctgatctgg taaagtttcc ccttaactta aataaatatc ttttcccctt
    13621 cctgtctttc gtaaaagcag ggcagttgca cagggagcga cactacgacg ccatctgggc
    13681 aatgatggct aattatgccg gctttggcgc gatgttcttt aagacctttc atccttctgt
    13741 gaaatattta ttaacactac aggagggcga tcctatcgat tatattaaaa gaagagtgag
    13801 atttgtttac cctctttttg taagaatttt tacaaaagct gatttcgttc aaccgctctc
    13861 aaaatatcta gccggttttg ccagagatat gggttacaaa gggccaatga agatcgtgcc
    13921 aaacgccgtg aataccaaac atttttctca agactttaaa gcagaagaac ttaatactct
    13981 taaaaataaa ttaggcaaaa aagaaggtga tattaatata atcaccacct cgcgcctcgt
    14041 tttgaaaaac gccgctgatg atgtgataag gtcacttgtt ttccttccgg acaattataa
    14101 cttcattata ttgggcattg gtcccgatat ggaaatgttg aaagatcttg caaagaaaga
    14161 aggggtcgcg aatagggtta aattcctggg gcaagttgat cacaaagaga tgcctaagta
    14221 tttgaaggtg tcggatgttt ttattcgtcc atctctttcg gagggttttg gcaattcatt
    14281 catagaagcc atggcggctg atatcccggt cgtcgccacg caagccggcg gtatacctga
    14341 ttttttgttc gaccctgata tcactccgga tattcctccg acgggattat tttgcaaaat
    14401 cagagatcca aaagatatcg caagacagat aaaaagattt atggatgatg ttccgctacg
    14461 agaaaaaata ttaaagaatg ccaaaaaaat ggtgatggca aaatatgatt ggagtatgat
    14521 agcgggcgat atggcggaga ttttcaaaag cttatgataa aaaaagtata cagaaaaata
    14581 aacgaaaagg ccgaagaaaa attcccgacc gcatacaagt cgctttttaa gaacaggatt
    14641 tttataaaat acgtttccgc cggtttcgtc gcggcttttg tggacctcgg ctcgctctac
    14701 ttgttcgtag aatatatttt ttatcacaga tacttgttgg ccgccgcttc ggcattcatc
    14761 ttcgcgttcg tcttcggcgc aatgttgcag aaattctgga cgttccagga caagaacact
    14821 tccggcatgg gattccagat ggtcatatat atcgtccttg ctattgtgaa cctcatccta
    14881 aacttgctca tcatattcgc gctcgttgag acgctcgata tgtggtatat tctcgcgcag
    14941 agcatctcta tggcaatcct cgccacgatg aacttcattc tgtatagcag gttcgtattc
    15001 aagaagtcgc tcatggtgcc ggggagcgtt cttcttgccg cgggcatatt cccgcccgac
    15061 ataggagggc cggccactca tacacttaag ttcatgaaga gcttcaggga aaacggcata
    15121 aagaccggcg tcatcacata ttccgacaga aagattgagc cggagataga tgatggcctc
    15181 gatgtagaga gggtttcgag gaagttcccg agaggtctaa ggcatctcat atatttggcg
    15241 aaaatttttg ccgcctcgct ccgctacgaa acaatttacg ctcaggatat ttccgcagcc
    15301 ggtctgcccg cggcattcgt cgcgcgcctt gtcggcagga gatttatggt gagggtcgga
    15361 ggggacttgc tctgggaaag gttagccgag aaggggaaga ccaagcgctc cgtcataaac
    15421 tattacaacg aagaaaatta taaaaagcga tttttgtatc acgtcggcca gactgtcctt
    15481 atgagcgcgg agaaagtcat cgtcccgaca gacctcctcg cgaatattta taggaaccat
    15541 tacggcattg tgagggataa tattttagtt ttgccgaacc caatccctgc gaagtgcgaa
    15601 acggcacagt tagacagaaa ggcgaccatc gggaaaaata ttctgttcgc cggtagattt
    15661 ataaaataca aaaacctaga caagctcatc atcgccttcg cgaatattta tgaagagatc
    15721 gctcctgcga agctcatcct tgtcggcgac ggtccggaaa agagagacct cgtccgcctt
    15781 gtaaaaaggc tcaacatcag aaaccaggtc atcttcaagg cgaagctccc tcatggcaat
    15841 cttttggaag agataagaga atgttcggtg tgcgtcgctc ccgctcttac cgagttcaac
    15901 cctaatttta tcttggaatg tttgtcttgc ggtaagccgg tcatcattac caaggagaac
    15961 ggcctttcga tcaaattgcc cgagcgattc acctggaatg cgatggacat caaagacata
    16021 gagaacaagc tcacagacgt agtgaacgcc ggcgctaccg caggggagga aatatcagaa
    16081 ataattcaga agacgaagac cacctcctgg gatgatataa tacgagagca tttggaatta
    16141 tttaaaaata gctaaagaaa gatgacgcca aacgccaaag atttaaacgt gctgatgctt
    16201 gggagcgata tgagcatttt gaagccggga agcgatgctt atcgcagaat gaaagaatac
    16261 ggctctctcg tgaacaggct agatatcgcc atctataacg gccatttaag aggcggaaat
    16321 gaggataacg tttatcagat agcacccaac gttttcgctc atcctaggtc atttttgggc
    16381 cttttagggc tatttagggt cagttctagg aagctttctt ttgtcctaaa tcatagggta
    16441 gatctcatca cctcacagga ccctttcatc cttggcctta taggggagaa gctcgccaaa
    16501 tggctaaata caggattcca ggctcaaata cacacagacc ttatgagccc atatttcagg
    16561 agggagtcgc tatacaatcg acttaaagtc atgatcgcaa agtctatcct aaagagggcc
    16621 accggtgtgc gggtggtgtc acaaaagata aaagactcga tcagaaaaat tagaacaaaa
    16681 gccgagccgg tcgtcctgcc gatctggacc gatattgagg cgatgaagaa gtcgcccgtc
    16741 agaattaact tgaaagaaaa ataccctcaa ttcaaaaaaa tattcttgat ggcttcaagg
    16801 ctcactgttg agaaaaatat tttcttggcg gtagatgcta tgaaaaatat cttgaaggag
    16861 aggcacgatg ttggcctcgt gatagtaggc gaagggccat ataggaaact gatagaaggg
    16921 ctcgcgcgcg gagaaggcct gcagaaaaat attatctttg aagattggtc cgaagacttg
    16981 ccttcgtatt acaaaacctg cgacgtattc ctaaacacct caaactatga aggatacggg
    17041 aggacgatca tcgaagcatt gtccgcgggg acacctgtcg tgacgacaga cgtgggcata
    17101 gccaaggagt tcgtgcgggg cggcgagaac ggcttcgtgg tgcctgttgg ctcaaagaaa
    17161 gaattggaaa aagttttgaa aaagatagta ggcgaggatg acgtgcttgc ggacctaaaa
    17221 gccggcacga aaaaaacgga gcttcccctc ggcacgaaag aagaatattt ggaagcgtat
    17281 gtgaagggat gggttgaggc gacttcatcc cgttgagtaa aattttatgc ataaaattta
    17341 aaaactctct ttataaaaag agagtttttt actcaatggg gtaaaataat tccttctctt
    17401 gtcattgcga gcgaagcgaa gcaatccagt aaaaacaaaa attcaccctg ttggataaaa
    17461 ttttatacat atgtcattcc cgcccccgat cgcggtcgag ggtaaactcc ggcgggaatc
    17521 cagttttttg tctattgagc agaacttttg aagctgattt agctgcataa taaattatca
    17581 cctgaaaaaa tccagatatt ttcttattcc tttcttattt ttttggtgta ttatctcctg
    17641 taatttcggc aacaattctt tcatcgcttt ttcgccggct aaaattttat cttgtccgtt
    17701 ggtaaattga taccagtagg tttttccgac atctatgtcg ataactatat cagcatccag
    17761 cacatcagca cgcgctaaat ggtggcgtaa aatatttaaa gaatcattcg caatatcata
    17821 ccagccgggg ctccagtttt cgtcaaaata atgtttatcc aaattaacgg caataacaat
    17881 atcagcaccc atatttctaa ctatttctac cgggaccggc gctgataagc cgccatcagc
    17941 caatattctt ccttctattt caactggttt aaaaattagc gggatagaaa tacttgctct
    18001 gatagccgtt gccattttac tcttgtccaa aattaccgat tcccctgtct ttaggtccgt
    18061 ggctactgcc gcaaaaggta ttttgcagtc ctcgaatttt ttttcatcaa tatgtttttt
    18121 aataaaggct gttacttttt ctccactgat tactccgctt ttaaaactag gctcaaatag
    18181 catagaaaaa agattgcgcc aatcggtatt cagggcaatt tcccctatct ctttgatgtc
    18241 tatgcccgac gcgtagaaac ctccaatcag ggcgccgata cttgaaccgg caataaagtc
    18301 aatcggtata ttgttctcct caagagcttt tataacacca atatgcgcta gccctctggc
    18361 gctaccgctt cctaatgcta agcctatttt tggtcttttt gtttctgcca tactgattat
    18421 ttatcagata ttttttctta aactcaaagt aatcttaaaa atattatttt tatgcacaat
    18481 taaattgtcc gtttattttg tctcttgtaa ttttattata ctcatttcgg ccggttttga
    18541 taataatttt ttaaaaacgt ccccatacag gttttgtatt aaagagaggg gaggtgctgt
    18601 aaaaataaca aaacaaaatt tagccgcgag aaagctttct tttcgatata tacataatat
    18661 actaggttca tatatggcgg ttataacaaa aattaaagaa acggatattt acctacagat
    18721 agaggaccag atagaacggc gtgtttttgt ttttatagat gcgtcaaatc tctgggaggc
    18781 tcaaaaggct aaggggaaga tgttcgatta tgaaaaatta aaaagatttc ttacgaataa
    18841 atttaggtcc ccaaatctaa aaatctttta ctatagcgcc tatccggcag agggtactag
    18901 agattatagt ctggataaaa aacaccaatt ttttacttat ttaaaaaaag gtttaaattt
    18961 tgcagtaatt aagaaggagt taaaaagaat cagtatatct aacgcagagg gacaagtagt
    19021 caaggaaaag gggaatatgg atgttgaaat aacgatagat gcagttcacc atttgaataa
    19081 atttgatgtt gcggtgtttt tctcgggaga ttctgatttt cttgcgcttg ttacatattt
    19141 gcgccaacgc aataaacaag tattcatttt ctcatctaaa aataatattt cagaagagtt
    19201 gagaacaggc ggggacgggt attttgatgt tttgagactt gaagatgata tctggggaag
    19261 agatctgaag catcgtgata ttaatactta acacaaagcc accctcctcg cggagggtgg
    19321 ctcttggatg taataccctt tcagttctta actgaaccga aagacatgta agagtatata
    19381 tataatattt taaaaaagca agttcgtttg gggataatta aaaagcacac tttttattta
    19441 tagattgccc tcgctgtcgc tattttctgt gattgtccag agagaataat atcggctgta
    19501 atatttatga gtagctaccg caccggctcc cgtctgtgat ttaaaaagat agcccgaacc
    19561 catctgttcc gcaaagtcgt atcccttatc tttcatgaaa gatttaataa tctcagagct
    19621 gtcacttttt gtaatatatt tattttcttc aatatctaat tttacgatat tctgttctcc
    19681 aaaagttagc tgtgcgatgc ctttaatttg cggccacgga ttgccttctt gaaagatgac
    19741 tttagagcaa ttataaataa gcgccccaag agcaaaactg acgatggcga gtattattgt
    19801 tttgtttttt aacataattt tcaaaagcgt tttttattat aaagaaaata aatcgtcagt
    19861 cgacatttta agttcccgcg ctatgcttag ggccaatttg agagaaggat tgtatttgcc
    19921 ctgttcaaga aaaacaatag tctctctcct tactccaact ttttcggcca gctcctcttg
    19981 agtaatgccc agtttggttc tgaatttttt aatctcgttt ttcatcttta gaatcgtatt
    20041 tactgtaata tttgaaaatg gccgagtagg taagcatcaa aatacaaatc gagaaagcca
    20101 gcgtcacggc aatggtttca taaagaggat ttgtgtcgct aagagcatac agcgcgaaca
    20161 tgataattgc tccgacagaa gacgtcagct gaatcgcaag caacgcagat ctgccgccaa
    20221 tttggtaatc tctctcgtct gctataattt ggaccacctt tttcctcaga taaaagaggg
    20281 tgagggatga taaccccaag accataattg gtatgaaaaa attatgaata accagagact
    20341 ggctaaatat gaatgcggtg acgaaaacta tccccaacct tattttattg tatgttttta
    20401 atgtcatatt atttgttaat tatttctaac tatctatatg ttagaaaaat ataacattct
    20461 cgtcaagcaa aacaggcaag aatcggagtt atctcaacag aatagtcgcc tcgatggtat
    20521 cggataagtt tatcaccctt aaagatggtt ttttatcatc tcttatctaa gggcataaag
    20581 gaaatataga ataagtgtct tctatgcttg taaacaaatc aaaaccccca gacccagaga
    20641 tactcttcgg gattttaata atacttattt tgtcacagat taagcgtcgt tcgataaaaa
    20701 gtactatcta cgggtataat attacgaagc ccgaattgaa aagatggctt aacttcgcga
    20761 ttattgataa aaatgcgcag caattaaggg gttatcataa agtttactca gaatggtttg
    20821 agacaccaag tccggggtct attaagagta aaaacgcagt ggaatatttg atttaaacct
    20881 tattcaatat ttctacaaac ttatctatat gtttcgtgag ttccgagaat gcagacggtg
    20941 actccttgtc ggcgtgcaat aggtttgagt aattgcagta tttgtatatg tttgcgattg
    21001 tttccaaatc ttcatcagta agcttcgcca tcttattttt tccttgcttt aagtttcgtg
    21061 taatgtcttc gaactctcgt tctttatccc acatcaacaa atcttgcttg ataaacctct
    21121 caacggcaag tcgcaaaagt tgcccgtatt tcaaggcgat ttcttctggc ctcaacgtgc
    21181 cattttggga attctggctg atttcttgat tgcattgtcg tacttctgcg actaaatcaa
    21241 aacctggatc aaaatacata aaactgccac caaactgttc ggcgtttttt aaaacgccga
    21301 atttagccgc gtttccgcac ttcgtcaaat acttaaaaaa tagagggtgg tgagtaaata
    21361 ccaaaacttg agagaactcc tgtgtttttt gatatattag ctcggcaagg attttgaggt
    21421 tcggcgagtc caaactggta atcgggtcgt caaataccaa aactttcgtg tttttgtttt
    21481 gcaggttctc atttactgcg aagaagaaag cgagagagat aagttgtcgc tcgccctccg
    21541 ataaattctt tttcatatct cgttcattgt ccttttggtc tttgatttta aacgaaaaag
    21601 aatactcttt tgtctgcgta ttcccgggta catgctctag ggtaaaactt aagttgaact
    21661 ttccgagtat ctctatcatt ctagtgatga catttttggg tattgtggtg gcgagatacg
    21721 acctcaagtc ctcttgcgct ttttttagtg cctccgatag ctccttctgt ttttccaaaa
    21781 cttcgttttg atttttgatt tggccaacca cgtcgtttct caggaaatcg gtcagtctat
    21841 ccaattccat tttcttttgg gtcttttcct gtatctcgct ggtgattttg ctctggtcgg
    21901 agtatttgcc cttgaaatcg ctgatgagtt tattcttttc ggtgatgagg tcgctcacac
    21961 ccttgattga tttttcaact tcttgaacaa ctaaggtaac cgcatcataa agtttttccc
    22021 cgttcacttg ctcccgttca atttttttca aagattctag cgcgataata aggtcgtcta
    22081 tgtcttttgt tgatacagag ctgaacttcc ccatttgttc cgttttttcg tccagtttgt
    22141 agatgtcttg cactgcaaaa tcagttttta ccttttctag ggcgtcaaaa attgccgtca
    22201 cctttgtagg aagtgagatt atgttcatct ttattgtttg aagctcgttt tccgacattt
    22261 caatgtcggt caaaaacttc tttttctcgg tctcatatgt ctgatcaaaa gccgctcgat
    22321 aatactcaat aacccttgtc gcgttggtga gcggttgcat acaaagcggg cagttttcgt
    22381 gcgtgtagtt ttctggaatt tcgctctttg cggattcaat aaaccgcttg tgctttgcaa
    22441 agtgtatttt tatcgcttcg tccgcttggt cttgcgcctt ttctttgatt tggcgataga
    22501 acagttcggt aactacctcc cttgtgggaa gtgcgtttgg gaataccacc tgcccaaccg
    22561 tggacaagcg gtttatttcg gcatatttgg tgttcaattt ttgcaacgtt gccaaatcct
    22621 cctcaagttt ttttagttct gcctgtgtcc ctattatttt ttgcagtttt gcttgattgt
    22681 ctgcattttt gtaggtttga aaaaattcct tgtccttttc attaaatggt tttgccaaaa
    22741 tattggcgaa ggcgttttgc aatttttcta attcatcttt ctttccctgt gttgcaattt
    22801 tgagttcgtt cgccttcttg tctaggtcaa taataagttt ccctgctttt tgcgtatgtg
    22861 cgccttgctg taggttgcta gatcttatgc cgtgcgtgtg gatattgtcg ttaatgaaat
    22921 caacatcaaa aaacaagaag gaatttttat caacctgacc tgaccaactg ttgttttcgt
    22981 atttataagt ttggttgtta gcgccgtcgc atatctcaag ttctgcgaga gtaggtggag
    23041 taatttgaaa atcttgattt tgagagacgc tttttaagat atcgcaagtt gaggatttgc
    23101 cagagccgtt ttcgccaaaa atcacatcaa acttggcgaa tacctgttct ttgagttcgt
    23161 ctacattatt tttcttatca acaagtctat atttgcaaaa ctttgcccac tcaaaatccg
    23221 caaatgattt gtatcccttt attgcgttga ttttgcagac ttgcatatag atatttactg
    23281 attgcctttc gcccgattgt gggtcttaca cagcatctca cagtttttaa tattagtttt
    23341 tccaccctta cttagtccaa ccatagaatt tgtgcaatag attattaata gctttattta
    23401 aagatagtct aataaagaag agcctaattt tttaatgaca tcaacaacaa gggcattgcc
    23461 cataatgaaa gctctttttg tagcaggtgt ctctttgcca tttttcataa atttagtatg
    23521 cccagccgga aacatgttta gtttttccaa ttcctctggt gtgagtctgc gtaatttacc
    23581 agatttcgtt ttaataacat gcttaaagcg agatggtgat gaacctcctt ccccggtaac
    23641 aattgttctt gatggtttat tcaaagggtc agggaacacc atcgaacctt cagaataatt
    23701 ataaatatgg ccagatttag tttttctttc ctccgttttt ccacctttta gatactccca
    23761 tcgtggcata tctttcatgt cgatgaaaaa ttcttttggg atattttttt catctgattc
    23821 tatgatatct ttcagaatag caaattcttt cctatggtca ggagtaactt tttttgtata
    23881 tacttttccg tctatcatta cacccgcaat ttcaaaggga cttttagtgg gcttaggtaa
    23941 agggaaattt ttagatagtt ttgctaaatc tttttccaaa ttaaattggt cacaaagacc
    24001 cgttgtcttt ttaataggaa aagctttagc tataacgcca tcactatcaa cccatctatc
    24061 tatatttttt tctgctttta cttttttata aatacctgtt ccttttttgt atcctaaaat
    24121 ataaacacgg cgtcttcttt ggggcattcc atattctgcg gcattaataa cacgccattc
    24181 gacaatatac cccagattgt ttagcgaaga gagcataatc gcaaaatcac gacctctttg
    24241 agctgaaggg gatttcagca acctgtctac attttccagc ataagaaacg atggcgcgtt
    24301 tttacctttt ctttcaagaa ttttatatat agaccaccag agaacgccct tcttgccgtt
    24361 taatcctttc gcctggctcg ccactcgagc aactgagtaa tcttggcacg gaaaaccgcc
    24421 caccaataaa tcgtgatccc gtatttcatt gaattttttt tcaatcactt cttgaatgtc
    24481 ttcatttgag tgaccttctt cgccaaaatt atatgtatat atttccgaag catgttgggt
    24541 tttggcgcca ggttcccatt ggctactcca aacaacctca tattttgagt tcttgtcact
    24601 agcagactct agcccgagcc tgaatccacc aacaccagca aataactcag ctactcgtat
    24661 ccttttcata tagaacctat attaacaaat caaaagaaat tgatcaatct tgatcaattt
    24721 aattttctat ggcctgctgt atatactttg cgttgagcca aaaacacctt tttgtttcca
    24781 tcgttccctg cggagtttta attgtatctt ctgcattttt accatgaggc ctaacatgag
    24841 caacgctgct tcccgtgatt tttggcaaat ctttatattt accctcattt atacaatcga
    24901 tggtctcttg ccaaaccctt tccgcttctg gcaaatcttt cattgggaaa ttccaaaaca
    24961 ttgttttctt taaaattatt tcatcgctat tttttatttt ttgaaaaatt acaaataaga
    25021 atttctttgt ttctagctgc gcgtgaaaat ccgacatcgt ttcttctttg tcatcatacc
    25081 aaacttgtgt aataaggtcg ttgtaatcaa acgcagggaa tgaaattgat tctctaagtg
    25141 tccctgtgtg ttcaagggtt atgaccttga gggtcacatt ggctttttct aattcttcaa
    25201 ttttgccgcc gtctattcct atgattttat ttataagtag tctcttgtat ccctttgctc
    25261 gtttatttaa gctgagaccc aaagaagaaa ttatttgagt atccgtcttg ccaacataag
    25321 gagaaaatat ttcttttatt gcatcttcaa ttgtctcaat tctgcgttgt ttcttaaaaa
    25381 ttgatttagt atcaactttt ttgcctaaaa tttctttttg aattaggtaa tttagatatt
    25441 gttgcttgag tgagaaagcc cttggtttgg cagggatacg atttctaggt tggtccctta
    25501 caactctgct attagctgcc ttggtacacg ctccaaggta ataagtatct ccctccgaga
    25561 gtagatgggc ttcgcctcta ctaattttgg caacgatata ttcccaatcc ttttgaattt
    25621 ggaatatgtc ctctttcgaa atatcctcga gcaaattaag taaataagtg aacttaaact
    25681 catggtcaat aatactttga ctttgaatcc atagataaaa catgagaaga accagtttgt
    25741 tctttttcag aaaagaactt gattcccatt tttcatgaac tatcttgtca taatctatca
    25801 tcgaaaagac tagtctttct tttgagacaa attgcttctt cggacttttc tttagggggg
    25861 tcgttttaag ctctactcct gcaatgctaa aatcagcctc actcttgcca tcgggctttt
    25921 taccgaaaac ataccattca attaattctc cgagaagacc tttccttcta tttttataat
    25981 ttaaaatatc attttcaact tttacgacgt cttctttcgg gataatttca cgcaaagatt
    26041 tattcaaaat gatttgagcc tgtttaagaa cttcctcttt tgttttgtag tcagtagtat
    26101 tcattcttct atattaaatc gataatactt gaaaacattt tttctgtcac aatgacatgg
    26161 tcaacaatat gaatatctag tatctttcct gcatgtacta acttttttgt aatttcaata
    26221 tcgtcttgag acgattctgt gtttccagat ggatgattgt gggcgagcat aatagaagcg
    26281 gcattgtttt ttatggcaga ctcaaaaact tctctagggt ggacgaggct ctcattaagg
    26341 gtcccgatag agattatctc tcttcgtatt tcttggtttc tgctgtctag ataaaaaacc
    26401 acaaaatgtt cttttttgct ggcacgaata tccggcatcc tttcccaaac atctttaggg
    26461 gacagcagga ttgaagtctt tttgtctttt aaaacccttc gtcctaattc aaagcaagaa
    26521 ataatttcgc ttgccttggc tttcccaagc cccttgattt caagtaaatc ttttagggtc
    26581 actttatcaa taccaacctt agaaatagtg cttaaaatct tttttgatag ctcaagaaca
    26641 cttagccctt taataccaga ccccagaaga atagccaaca gctcgtggtc taaaagtttt
    26701 tctgggccat atttttcaag tttttcgcga ggcctatcaa tcttgggtaa atctttgatt
    26761 ttcatactta gactatctca tattttaatt ttttttcaaa ttaataataa gcgtataggt
    26821 gtttttgggc atagaaaccc ttgaataacc ttgggataaa taatcattgt atatttataa
    26881 ataaaaaatg cccccattaa atccgggggc attttttata caagttctac ttttttccct
    26941 agagcatgag caaccttgtt tagcgtcaca agagaaaaag tgtgattacc gctttctatg
    27001 cgcgcaataa cggattgtgg cattcgtgcc ttgtcggcaa atcctttttg cgtcattctt
    27061 ccttgcgttc ttaatgtttt tatccggctt gccatcttta gtctgaagag ctcctcatta
    27121 tatgaattta caaaattttt tgatttagat agttctttaa atagtttgtc gaatgatttt
    27181 attttattta tattttttat atttttattt tgcattttta tattttgttt ttcgatcaaa
    27241 tatttatttt ttggtttttg tatttatgta ttttttgaag attgtttcag cattacgtat
    27301 ttctctcacg ggggtttttg cgctcttttt gataaagcca gagacaaaac aaattgtatc
    27361 gtggtcaata aagaataata ttctatattt atttatcttc agttcgtgaa tagcgcgccc
    27421 aagtttccta atatttacca cactcatgtc catggacatc attttgatgg tggccattat
    27481 ctttgctggc gcatcatgtg gtaatgtttt gataaaacat tcagccccgc ccagaaatgc
    27541 taatttataa gtttgcactt attttttaaa tattgattgc taaagttcga ccaagtgcta
    27601 gtatagcaaa attgctataa ttaagtcaag ggtgggtgtt aataaagttg cgaaatttat
    27661 atggtgctat tgaaaacaaa ataacgatta aataacttac caagaaaccc gccatccggc
    27721 ggatttaatt ttttgtggtg agattctcgg tccccatggg acttaagcac ccgtttccga
    27781 tttgtttgga caacaggtcc tcataaataa cggagaatgg cgttcgaatc tctcaccttc
    27841 gggctacttg cgattccttt tttgaaaatg agatgttgcc gctacctctt tcttttcgat
    27901 tttcattgtt gtgggttacc ttaagataat ctatatatca gcctttcgga gattatttat
    27961 tataccaagc tttaattatt tgttccgccg gtttatttct aaagctggct gaaatagata
    28021 tgcgcggtct tacttccgga tcgagggcgt cgtcccaagt aaaatttgcc gcattgattc
    28081 ttgagatatt gttagtcttg ttgagtgatt caaatagata attatatgct tcagattgag
    28141 attcataatc tttttctata ttttttattt cttgattatt gatatctaaa aattctaccg
    28201 ggttattata tagcccattt ttataactag acggagcaaa gaatattgat aatttaatcc
    28261 caagcttttg ggcttttcta tctaaatccg agaccatctt gctcatatct tgtttaatct
    28321 gatttttgtc ggggctttga tatttttcta atattttata aattcttgct tctgttatgt
    28381 cagctttttt ataataatca tatttattcc aattttcttt accattgttt ccgtcaacca
    28441 agccgtaaat atttatttcc gccaagattt ttcctgaaaa attgttttta acttcatcta
    28501 tcaaatcgct ccacaattta tcggcaagct cttcatgacc aaagaattga gggtcctgga
    28561 aggtggggga gatagacata atatctactc ctgctcggct atatagcttg gctttttcaa
    28621 ctaggcgatt ttcccatttt tgaaaataat catttatcca ttcttcggaa tggttttcat
    28681 taaattctga aaaatttttt gaaacgtcgg ccgaaattgt tcctctgata ccggaaataa
    28741 tgagattctt catgtctata aaagtgagat ttctttttat gcccagtttt aatcctcttt
    28801 cgtgagccgc tgtgacaagt ttttttaaat cgttttcatt tatagactcg tcacgcatat
    28861 cattactgaa tacgtcatct tctattgtgt aatctgtgct actaaaatct tgctcatccc
    28921 cataaaccgc acggtcaaaa tcgtgaacgt atacctcttc tgctccgagc gactttattc
    28981 tatcgaaaga gctttcaatg ttggcttgag tattctccac catccccata ttgtaatttc
    29041 tcccccaggt atcatacata tttatcgcct taatctgtga aattcttgtc ggtttggtat
    29101 caactagata ttcatatatt ttatattcag aagaacacca tggcggcatc ggccacgggc
    29161 cctttggcgg acagatttga gcaaaaaaca ttacaaccac tattgcactt attagaccga
    29221 ttgatataag aaaaattttt ttcataaata aattatacta ttgataaaat aaaagaaaat
    29281 cctcacaaaa atttcgctcc gacgttcaaa tctctcaccc ttcctgtaaa tagcaaaccg
    29341 cccaaacgag aaatcgtttg ggcggatctc gaggagagat ttgtcccgct ttttctaatt
    29401 cgctcctgcc taccggcagg caggtcgctc ataagaacgc tggacgcggc tcgcccacgg
    29461 ccttcggccg gctctgcggg ctccgccgtt caaatctctc accctctttg caaaacgaca
    29521 aaccacccaa atgcttacgc atctgggtgg gtcgtttgcg gagggtgaga gatttgaact
    29581 ctcgaggccc gttaagacct acgtcctttc cagggacgcg cactagacca ctatgcgaac
    29641 cctccaaaat tacctatatt tagttattct ttcgttccaa taatatagat tattttttga
    29701 ttaaagtcta ttaaaacaga tgatataatc ttcgtatatg aaaaagatcg tagtgaacga
    29761 tattatgcag aaggggtatc tgtacgagct cacggagccc gtcgggaaga attttgcaaa
    29821 agatttcaag ccacagctca cgccgaaaga gatgcttgcc ctcggtgtgt ttggtggtag
    29881 gtatatgacc gactgcaaaa aggagtttcc ggccgattgg tacaaggagg cgaagctttg
    29941 ccacgagagg cacgaccccg aattgaatta ttttaaggtg aacgcgagcc agccgctttc
    30001 tgcgtggagg gcgaagggtt ggataaatga aaagcacgat cctcgcggat ggtttcagtg
    30061 gtattgccga tactatatgg gcaggcgctt gccggaagaa gatgagaaac agataaagag
    30121 gtggcgagct atcgcgagac acattgcgca ggtgaagaaa aattgccggg caggggattt
    30181 gaattgtagg aggaggcaga ggcaatctct tctccattgg gcgatagata gtcgaaagtt
    30241 ataaaccatt tatttaggaa attgtagtaa taaaaaagag gggagccgtg tggcgcccct
    30301 ccgtaggtat gtttaacgtc ttaccttaaa gacacctggt gcagccgtcc ttgcaacggc
    30361 agactcgcgg gaccatgaat ctcttcatgc aagaccatct ccttcccgtg ttttgtgaga
    30421 gccgggagcg tttcttagcg cccttccctt gagccgacaa tataatatgg atgtttaata
    30481 atgtcaagcg acaaaggaac ttgtcccgcc aggaaggttc gaattagtta ttttcttact
    30541 taatttttta tctcccggaa tccgacttcg cctacgcaca agggaactaa tagattctat
    30601 ttagtaacct cctacgggac tgcgagcctc ctcttttaaa aagaggaggc tcttgcgttt
    30661 tgttttaaat atggtataaa agacgtttat taaacatgca aacaagagcc ataaaactag
    30721 atattccttt tgtgaagcag ataaatgact atttctgcgg gccggccgcg cttcagatgg
    30781 tttttggcta tttcaattgt gagaagcccc aggtagagct cgccgagata atgaatacga
    30841 aagagaacgt cggcacgaag aaaaagaaaa tggttgaggc tgttttggaa cacgattttc
    30901 atta
//
LOCUS       DAOTEX010000002        28500 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3118
            NODE_1084_length_28500_cov_4.365372, whole genome shotgun sequence.
ACCESSION   DAOTEX010000002 DAOTEX010000000
VERSION     DAOTEX010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868142
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 28500)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28500)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 883
            CDSs (total)                      :: 833
            Genes (coding)                    :: 829
            CDSs (with protein)               :: 829
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28500
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1084_length_28500_cov_4.365372"
                     /isolate="AMR_MDS_3118"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="P5056_00175"
     CDS             complement(<1..358)
                     /locus_tag="P5056_00175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62186.1"
                     /translation="MKKEEEILRKEEKILNELEQEKKDLKSLKKNQNILNLLILVTVA
                     GVLGGGIYWQISSGRVYIEKSQILGDEVSLSPQTNGVLMENFVKEGDYVGENAPVARV
                     GNELVTSKVAGVVISVR"
     gene            complement(397..1944)
                     /locus_tag="P5056_00180"
     CDS             complement(397..1944)
                     /locus_tag="P5056_00180"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRY62187.1"
                     /translation="MNQQMKKPIIAATIVLLGLVAFVFYAMSHSGIQNGNSVFVVARG
                     NIENVFKTTGQVKPKNGVDLAFERSGKVSAKYFNVGDKVSAGKTLVALENYDLVALLH
                     QAEANLKIQQAKLDELKRGARNEDVLIQQSRLAGAESALVEAKQNAIDKIQDAYTKSD
                     DAIRGKVDQFFSNPKSANPALTFVVPDLAKKEKLEFDRMVIETMLNDWYSDTLSLGAS
                     GDVSGPCDKAFSNLRQIKSFLDDLALVINALSPSSQLSQTTLAGWRADITLVRSNINT
                     AISSITAAREKLEGAKMNYDIASAGTKMMAAGATPEQLRAQEGQVDSANAALESARAQ
                     LAKTIIRAPFSGEIAKDDVVVGQLAVPNVPLISIVSTNQFDIQTNIPESGISRVKVGD
                     AAKVTLDAYNNNTFFDARIISIDSAQSVVDGVPVYRAKLQFVNEDNKIRAGMTANINI
                     AGQTLQNVLYVPERSVFSREMKNLVFVVGKEGAAVEREVVAGARGTDGKVEIVSGVAE
                     GETVLVY"
     gene            complement(1946..2356)
                     /locus_tag="P5056_00185"
     CDS             complement(1946..2356)
                     /locus_tag="P5056_00185"
                     /inference="COORDINATES: protein motif:HMM:NF013231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRY62188.1"
                     /translation="MEIDEKLMSVIFETSRVVKARIQKASIGRSFSYGQMHVLNFIET
                     KGGANMKEVAGFLRVKPPTATAITDGLVKSGMLTRLGGRKDRRVVKMEVTIKGKKFLS
                     DSFKVASGELKNMFSCLGQKDKVSLIKILSKIKE"
     gene            complement(2508..3590)
                     /gene="rsgA"
                     /locus_tag="P5056_00190"
     CDS             complement(2508..3590)
                     /gene="rsgA"
                     /locus_tag="P5056_00190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HRY62189.1"
                     /translation="MDKNKNISLEDLGYTSFFETERNQLGLTEFSVARVVSEHKEAYV
                     VKNANGEFFSKITGRQMFEASGREDYPAVGDWVAITEMGEDTAVIRGILPRASMIKRK
                     YRDRTQVIATNIDVAFVVESVNRDYNLNRFERYFAIIENGGVKPAIILNKVDLLSKEE
                     SAEKIIELRNRFPDVDIISTSIVKNDGLDELQSYITKSKTYCFLGSSGVGKSSLINKL
                     LGNGAIKTEHVSSYSDRGKHVTTGRQMYFLQNGGIVIDNPGMREVGVADAGQGIDTFF
                     DEISCAAQRCRYNDCTHTHEPGCEVIKVVKSGELDKEKYSNYINLKKEAEYHEMNDVE
                     KREKERNFGKFLKRTKKELRDFGYKN"
     gene            complement(3679..3930)
                     /locus_tag="P5056_00195"
     CDS             complement(3679..3930)
                     /locus_tag="P5056_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HRY62190.1"
                     /translation="MPKQPTTYILECADKTLYVGSTNALEKRLHEHNHSKNGAHYTKI
                     RRPVVLKYTETFATLGEARSREAELKRLTRKKKLGLIRN"
     gene            complement(3934..4824)
                     /locus_tag="P5056_00200"
     CDS             complement(3934..4824)
                     /locus_tag="P5056_00200"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HRY62191.1"
                     /translation="MAQKEEAIIITEEVGAVTPVVNTPEVLPAPESTKILIVGDIQLD
                     RYIRKVGNMSGGDFIFSCIDPFLSDYNFVVGNLEGPITENTSISMGTVIGSPENYVFT
                     FPTTSAELLARHNIEVVNLGNNHIGNFGMSGFSLTKEFLNIAGVNYFGGIIGDEPIYS
                     RENISFVSYNEFGGQSPEKVAEIIKSEKQNGKIVIVYAHWGDEYIDSSPRLRGNATLF
                     AESGADLVVGSHPHIVLSSEHIGNTAVYYSLGNFVFDQYWNEEVSTGLTLALDIKDGK
                     INISEFPVTLHKDGRTCLKN"
     gene            complement(5072..5521)
                     /locus_tag="P5056_00205"
     CDS             complement(5072..5521)
                     /locus_tag="P5056_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62192.1"
                     /translation="MKIPKQCRKCKNDAIKEIVYGNPGHISDCSKIIGEMNSGRAKWH
                     CGHCHWEWGPKMGRYNTNRTKRGEIRKAIFTFNGYNYCGGIKRCGYLANRMVKEYKIK
                     KKFIKYSTADLMSCIFYEGRRAHFGIPPNRQYYLFLIKEVEKRLTEK"
     gene            complement(5518..6147)
                     /locus_tag="P5056_00210"
     CDS             complement(5518..6147)
                     /locus_tag="P5056_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62193.1"
                     /translation="MKARNTPDTIIEKIYNNQEKIFSNIDKESVDTYQWLNTAYNSGG
                     IDHNRVFQFVFRSYYRLDGAGLSQNQKRTFFDLMTNKIRDLEKILDALYQLPTLRGNN
                     TIQFSFATKLIHTIDNNEPIFDAEVSNVIQRRVAGNNKEQKIQSCVELYNFLKSLYLK
                     LLSDDKIKELISIFRKEFGVNKYQVSDIKILDFIVWTLGKLEKMENKIS"
     gene            complement(6246..6316)
                     /locus_tag="P5056_00215"
     tRNA            complement(6246..6316)
                     /locus_tag="P5056_00215"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6282..6284),aa:Gln,seq:ttg)
     gene            complement(6289..6561)
                     /locus_tag="P5056_00220"
     CDS             complement(6289..6561)
                     /locus_tag="P5056_00220"
                     /inference="COORDINATES: protein motif:HMM:NF013691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HRY62194.1"
                     /translation="MKAFLYILENTAKSHYVGITESEPEKRLIRHNKGDVFSTKYKRP
                     WKLIYVEEFENMKEARLRERQIKSWHGGNAFRKFLVKAAGSSNGRT"
     gene            complement(6735..7769)
                     /gene="dnaX"
                     /locus_tag="P5056_00225"
     CDS             complement(6735..7769)
                     /gene="dnaX"
                     /locus_tag="P5056_00225"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR02397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HRY62195.1"
                     /translation="MSIALYRKYRPKNFKEVLGQDNIVRALSGSIDEDRIFHAYLFIG
                     PRGTGKTSIARIFAKEIGTSEKDIYELDAASNTSVEDIREITDGARTLPFESKRKVYI
                     IDEVHMLSKSAFNAFLKTLEEPPAHVMFILATTEAHKVPDTIVSRCQSFTFKKPSNEI
                     LKKAVERVAKGEGLTIDKSSADLLALLGDGSFRDTIGLFEQVSNISKDKKITIDDIES
                     ITGSPRGEVIHSLIKEILEKNLEAALVLLRKIAIKNIDMKIFLKMLLRDIRLVMLLKF
                     APEMEKEILSEVSDDEAKVFKELKGHKNIAVLPQALKELIYANHELGHGSIPELPIEL
                     ALIRLLGQTN"
     gene            complement(7817..8161)
                     /locus_tag="P5056_00230"
     CDS             complement(7817..8161)
                     /locus_tag="P5056_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012529297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HRY62196.1"
                     /translation="MKIEKFEDILAWQKSKELTLSIYKTLKDCRDYSFRDQIQRAAVS
                     VMNNIAEGFERHTNKEFISFLYIAKGSCGEVRSMLDLGLDLGYFTKKEHDNFKQVSID
                     ASKLISGFIKVL"
     gene            complement(8204..10288)
                     /gene="argS"
                     /locus_tag="P5056_00235"
     CDS             complement(8204..10288)
                     /gene="argS"
                     /locus_tag="P5056_00235"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HRY62197.1"
                     /translation="MQQNLLEVWNSSISEQVNKKKDEVIKKREAAGYVPLAEYVFEDF
                     KKQLRDTNKDVEGFDTVEIEFIDREKFNADIAIKVPHLMKTYGIPTYTKEYIPQIISN
                     LERSSFVKEHIEKITPTGIYINLLMKPGCFSCLINNVLVLGDRYGYSDKHRGESIVLD
                     YSSPNMAKHLHAGHVRSTIIGEVLAKIYEATGYTVHRLNYLNDWGGMGAIMEAYLRLK
                     DKNAWSLDLSGNDMLYQVYLIVRTAEKMSTNKSEFDKFLLGTDQNDVIKKLLGDFADF
                     AEYKNKYVEFVEAGKERFRKLEDGGKDEFALWQKMREWSLSEFNKFYEILNIKHDYLL
                     GESFYAKSGADLVKELLKTGGAVQFTQELADVEIKKAQLAFEAGTIKKAVLEKLLEEI
                     SHDIGAYVIMLPSGSRMVIMRADGATIYATRDLVSAKHRLETFIPSRLVYEVGQEQTE
                     HFKHLFEAVLTLNLNKGANIGLEHVYHGFYVDAATGQKLSSREGAQNVVSLVEESIKY
                     FRNKYEERSTGEKAQNFTDEEKEKNSKLLAVGSIAFNDIKQDKKFPIAFHKELDKNIR
                     NFEESGGAYIMYSIARARSILRKSDKKVEDINIDTLDFSKLENVEISLIKKVAELPIV
                     VRKAAETDNPATLAEHLLHIANDYNSYYENFDVLESGKLVYPHRLLISSAVATAMANG
                     LKLCHAEAPERI"
     gene            10434..10790
                     /locus_tag="P5056_00240"
     CDS             10434..10790
                     /locus_tag="P5056_00240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62198.1"
                     /translation="MKERYSEMETPLMLLGMFVVILLITLITTVIVGLFPRATARLMG
                     LCVYLGLLYKLIPAMNSAVPKEAPLLHIILAIIILFEATGIFVLMARFIMLGAMRDYR
                     NMYYNIKESQEDMSLS"
     gene            complement(10969..12015)
                     /locus_tag="P5056_00245"
     CDS             complement(10969..12015)
                     /locus_tag="P5056_00245"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HRY62199.1"
                     /translation="MSTSLYSTNILKIIMKNKNFWQKLKLTKRPFFCIAPMADVTDAP
                     FRKMFSKYGKPDVMWTEMVSCDGLMHEEKAEQKPRGSKYPARDRLALSFIYDESERPI
                     VAQIFGGDPETFRETAKYVRELGFDGIDINMGCPDHGINKQNAGANLIKSPKLAQEII
                     KATKEGAGDIPVSVKTRIGFNKNEIDTWIPAILSAKPAALTIHGRTRKEMSKVSAHWD
                     EVAKCVELAKDTDTVIIGNGDVKSLEEGRKRRDESGVDGIMVGRGIFGNPWFFNEKIK
                     KSDLPLSEILKVMLEHARLFEELLGGKKPFVLMRKHFGSYVGGFPGASELRAKLMTTE
                     CADEVEKVIKNFEL"
     gene            12169..13164
                     /locus_tag="P5056_00250"
     CDS             12169..13164
                     /locus_tag="P5056_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62200.1"
                     /translation="MGKRSKEKRKKQRRKDEIEESCTIPIEKKPPEAFRFNHMEFATA
                     DEAATAKFIFRNNIEKDEQELRKLVALIIKHNILRNNETEIRFARLLMKHNIRFEYSK
                     EIETQKIIQKEEYSETPETTAKDGETPSKPGVRQIDFWLPDGPIMVPFCHEPVQSFEV
                     KGGKLTMNCWRQRRELRDANYFTFIVLPQYISFWEKHGLLEPVSTRENNARGIYQKPA
                     CLMANHIPKKESSLKKKMVSVLRTEHIKFEYAEDVRGERLGDGAHRAIFFLKEPVKGF
                     WCGEMTQAIMLIEGDITMNDFERWQEVSETQNTFLAVKQFVRFWHGVGFLRQKAK"
     gene            13210..13854
                     /locus_tag="P5056_00255"
     CDS             13210..13854
                     /locus_tag="P5056_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013664999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HRY62201.1"
                     /translation="METLLKLAEFALHIDKYLVQIVANYGSETYLILFLTIFLETGMV
                     FTPFLPGDSLLFASGMITANSDLNIFFLLIILSVAAILGDSVNYSIGKYFSYRIKNGQ
                     NIPFVKQEYVEKTERFYEKYGNKTIFIARFVPVIRTMAPFFAGFGSLNYSSFIFYNIA
                     GGLSWVALFLFGGYYFGNVQIVKDNFSAAIMFVIIVSIIPAIVECIRESRLGKK"
     gene            complement(14232..14642)
                     /gene="rpsI"
                     /locus_tag="P5056_00260"
     CDS             complement(14232..14642)
                     /gene="rpsI"
                     /locus_tag="P5056_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009145170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HRY62202.1"
                     /translation="MAKTTEKYFEAVGRRKCSVARVRVWPASGTKSTVVVNEKDIKEY
                     WNTADSRQKITDLVNRADLKDKLKVSIKVNGGGMSSQVDAALLGIARAITKMDAEMRK
                     PLKKEGHLKRDPRVKERRKFGLKKARKAPQWSKR"
     gene            complement(14648..15001)
                     /gene="rplM"
                     /locus_tag="P5056_00265"
     CDS             complement(14648..15001)
                     /gene="rplM"
                     /locus_tag="P5056_00265"
                     /inference="COORDINATES: protein motif:HMM:TIGR01066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HRY62203.1"
                     /translation="MAKKEYTIDAINRTVGRVASEAAMLLMGKNSPEYESNIAPKVTV
                     KIINTGKAKIPAAKMKGKEYLRHSQYPGGQKSETMERVVAKFGYGEVFKRAVYGMLPK
                     NKLRAVMIKNLTVSE"
     gene            complement(15006..15356)
                     /gene="rplQ"
                     /locus_tag="P5056_00270"
     CDS             complement(15006..15356)
                     /gene="rplQ"
                     /locus_tag="P5056_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HRY62204.1"
                     /translation="MRHHNTNRKFGRETDQRTALLRSLACNLINYEKMITTEAKAKEI
                     RPFVEKLVTKAKEDNVATRRLVSQYLVSPKQTAKIFKEIAPKYKDRKGGYLRIVKLGQ
                     RGGDGSKMAIVEFV"
     gene            complement(15367..16359)
                     /locus_tag="P5056_00275"
     CDS             complement(15367..16359)
                     /locus_tag="P5056_00275"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF003519.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HRY62205.1"
                     /translation="MLDFNIVLPSKPRVFSETELKGVYEIDGLYPGYGHTLGNSLRRI
                     ILSSLPGATITSVKIEGADHEFSTLEGIKEDVIAIVLHLKKLRFKVDKDEPQVVYLKA
                     SKVGDVTAADIEETASVKILNPEQHIATITDKNTKLNIELTVEKGFGFVPKELINKDK
                     VEIGTIAVDAAFSPIRRVNYEVENMRVGDRTDYNRLRLFIETDGTLTPRQALERSIEI
                     MISQLKSVIGFVDEEETKEEVKKVEEVKEEVAEEKAAAPEVKAENQEEVLKMRIEDLK
                     LSGRTMKALSEASIRTVGGLARKKEDDLLEIAGLGEKGINEIKKVLGNLGITLK"
     gene            complement(16393..17013)
                     /gene="rpsD"
                     /locus_tag="P5056_00280"
     CDS             complement(16393..17013)
                     /gene="rpsD"
                     /locus_tag="P5056_00280"
                     /inference="COORDINATES: protein motif:HMM:TIGR01017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HRY62206.1"
                     /translation="MLRGPKYKVCRRVGGMVFPQCQSPRYTSTLTKEGARGGRKGRPK
                     PKSEYGTQFQEKQKAKFTYGVTERQFVNYVNASKSSKKGNPANMLCQALETRLDNVIF
                     RLGFASSRAFARQMVSHGHILVNGKRLNTPSYGVKLGDKISVRQSSQKSPLFAELKSK
                     LKDFKFPKWLKFDLDKSVAEVIYLPELADGDQTLNLNVVMEFYSRT"
     gene            complement(17036..17467)
                     /gene="rpsK"
                     /locus_tag="P5056_00285"
     CDS             complement(17036..17467)
                     /gene="rpsK"
                     /locus_tag="P5056_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012693975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HRY62207.1"
                     /translation="MSTDTKTVKNENAEIKDTKAAAPKVPRHKMSTGILFVQATYNNT
                     KVLLADSKGNAVAASSSGTLGFKGAKKGTPFAAAKVGELLGDKAKAMGVNEVDVVVSG
                     VGAGRESSIRAFSSKGIGILSITDKTPVPHNGPKPKKPRRV"
     gene            complement(17597..17977)
                     /gene="rpsM"
                     /locus_tag="P5056_00290"
     CDS             complement(17597..17977)
                     /gene="rpsM"
                     /locus_tag="P5056_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HRY62208.1"
                     /translation="MRISGLTIPDNKRLEVGLTTLYGIGRPIAQSILDKLGIDRGARP
                     GDLSEEKENALRKEVESRTIEGELKREVAGNIKRLKDIKCYRGLRHMKSLPSRGQRTK
                     TNSRTRRGNTRKTMGSGRKKVEKK"
     gene            complement(18006..18119)
                     /gene="rpmJ"
                     /locus_tag="P5056_00295"
     CDS             complement(18006..18119)
                     /gene="rpmJ"
                     /locus_tag="P5056_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012548601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HRY62209.1"
                     /translation="MKIRSSTKKMCAKCKSVRRKGRVYVICEIAKHKQRQG"
     gene            complement(18123..18353)
                     /gene="infA"
                     /locus_tag="P5056_00300"
     CDS             complement(18123..18353)
                     /gene="infA"
                     /locus_tag="P5056_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018963272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HRY62210.1"
                     /translation="MDIKVTNSSSTGAEVEGVVVESLPNTMFRVDIGNDKVILTYLSG
                     KMRINRIKILIGDKVRVQLDSHGERGRIVRRM"
     gene            complement(18566..19864)
                     /locus_tag="P5056_00305"
     CDS             complement(18566..19864)
                     /locus_tag="P5056_00305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62211.1"
                     /translation="MRKQPFKDFISGAGSRAERPVHKDLYEEVPRRRTPVSFSLDKKS
                     GKRFFSATSTKTIISVSTALLLAFIWFSSAYSSVVVKVTPISENVSMDDAFVAVSVKD
                     AVKDGVVFKTMKVEENDSLPIAATESKAVSQKASGEIVVYNNYSTASQKLVAGTRFES
                     SDGKIFKIDKAVTIPGKQTVSGKLVPGSLTVTVYAAEAGKEYNIKASDYTLPGLKGSP
                     RYTSVYGRGKGDMSGGYVGEMKIVSPTDVALTKKKLEDSLTKKLREKIASEVPAGYIV
                     YEDAMFVDFEDNIKQLGSEWEGDKVYLKMKGSAGAFMFDMKELATAMTKKKLPSLNNY
                     DVHSNELPALEFKILVKEGVSPDSSKMFTFKLNGDAKVTWNLDVDNFKKDLLGIKKEN
                     YQDIFKKYPTIEKAETIFNPSWSGSFPDEVGRITVELIED"
     gene            complement(19893..21116)
                     /locus_tag="P5056_00310"
     CDS             complement(19893..21116)
                     /locus_tag="P5056_00310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62212.1"
                     /translation="MINPFVFIKKEDVVVLDIRDGSVGGMILTVNKEGGGAPIKVFSV
                     RKEIPFQRSFNNKRLLASISQAVAFVALAIRDSGFKIPSKVFCILGPHLHASQVRIIK
                     SKYDKPVTISEEVLHSLILQDSKEFQQKHLLNTNASDSPNVMMEHKITQIKLNGYETS
                     SPIKKNASEIEISVFVSMAPGELLDSLKKNIFKNLHTNKIDFHSFTFALSDIVRKIAP
                     ERPSYVVVNIEDEISEVSVIRDGEVEDIASFPVGMSMLTRVIMDRFNLSNEQALSYLK
                     LYLSGSAEKDLSVKMVDAIDRAMVLWTDHFYETLRKISFHRLLPDIFYIATEETCKDV
                     FINFIQKAEVSGLLVTSRSPRVECLEPNLFAELCKNISSCPDELHLLVDAIFIDKFYR
                     QNIAHRWGEEKRYIL"
     gene            complement(21203..21901)
                     /locus_tag="P5056_00315"
     CDS             complement(21203..21901)
                     /locus_tag="P5056_00315"
                     /inference="COORDINATES: protein motif:HMM:NF039508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5701 family protein"
                     /protein_id="HRY62213.1"
                     /translation="MRKNSILVQTLFISALIISFSPALVFGIETIHVANFMDNVPRME
                     KIFDNQIAELRARGFGDEFLGDLMVQRTSVTVKAAKMDIAPGNIPFVPAISVGYGLTG
                     RCGRPKLKETDRKLDIVNFKQFYPLLGESNSHSGPYYVFDVSSGGDMLGLTPKEAAKK
                     LASLGRRPLNAIEGTFMFFLGGNAARNGMWFAGSRCNGKTPVVYIKGDKIRVDAESPD
                     NKYSDRGTVSCVKE"
     gene            complement(21995..23878)
                     /locus_tag="P5056_00320"
     CDS             complement(21995..23878)
                     /locus_tag="P5056_00320"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010165648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HRY62214.1"
                     /translation="MTSKEVRAKFLEFYRKKEHAIIASASLVPENDPTTLFTSSGMQP
                     LVPYLLGEKHPMGVRLANSQKSFRAEDIDDVGDNRHTTFFEMLGNWSLGDYFKKEQLP
                     WVFEFLTDEVGIDPNKLYVTVFAGDKDAGVERDEESVKIWKELFKKKGIDAKDVELIT
                     EERGGELGMQGGRIFYYGAKKNWWSRSGVPMNMPVGEPGGPDSEIFYEFIHIEHNPKF
                     GKYCHPNCDCGRFMEIGNSVFMEFQKQDDPPTGQAGGSFKKLPQINVDFGGGLERIVA
                     ASNDNPDIFEIDLIFDIIQAIKIVSCKNYEGADKVSMRIVADHVRSSVMMIADGVVPS
                     NSERGYILRRFLRRAVRHADKLGMKEGALATFASLVAEKYKGVYDNINTNLIAIASEI
                     QKEETRFRETLASGMKMFEKISKKDISGADAFVLFSSHGFPFEITAELAKEKGVKVDK
                     KGFEEEFRKHQELSRAGSEKKFKGGLGGLSEIETKYHTATHLLNAALKKVLGEHVYQK
                     GSNITVERLRFDFPNPAKVTDEEKKKVEDLVNQKIKEALPVSVEEMPLDKAREEGATG
                     IFGEKYPDVVTVYTIGEKNNYFSKEICGGPHVKNTSELDHFKIAKEEAVAAGVRRIKA
                     VLE"
     gene            complement(23905..23975)
                     /locus_tag="P5056_00325"
     tRNA            complement(23905..23975)
                     /locus_tag="P5056_00325"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(23941..23943),aa:Ala,seq:cgc)
     gene            24242..25231
                     /locus_tag="P5056_00330"
     CDS             24242..25231
                     /locus_tag="P5056_00330"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HRY62215.1"
                     /translation="MKKIPLIAQIVPFLLVPIICTLILMFTFCAHMTGDQIFGFANNG
                     AWVYGRCHNSVGQVFAVHTIRFKNPVVQAVMQIFLGVPQVTIYGSGFYFDKDYPYAAT
                     NSHVVAAPKMESETFARIMTGMRKGYRVYTDNSCTKAVVGGQIIEVEKDATQIPIPKV
                     EGDSEYDIVSEYFFIMGDHKKYPCSVISNFPDKDMAILKVLTDHWLPLPIANPDEARV
                     GEQVYAIGSPLAVLFNSFSDGVISGKDRDISALTSPLRYSASGFNTWIQITAHVNPGN
                     SGGPLVNRRGEVIGINTLSYNYLTSISGIYMANRIDLLKVPEFWLKKAPEEKK"
     gene            25373..26272
                     /locus_tag="P5056_00335"
     CDS             25373..26272
                     /locus_tag="P5056_00335"
                     /inference="COORDINATES: protein motif:HMM:NF024758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HRY62216.1"
                     /translation="MKRKDVILFLIIWLIATCLLTAVVIRDSSVTSPYNKHELTRREI
                     YLECKKSVGLLDVTHRAYFGDPRAQIFFALFPVDLDYGGSGTAWFFDKDRPYILTNAH
                     VVNCPNPIALFLSVWSKLPETHKNFLKKLGTIKMAVESTYVFIDHDGKKYSCKIIANL
                     DKLNNVDLAVLSVDIKKWKPLPIGNPDSIKVCDVVYVMGSPLGYVNTFNMGYVSAINR
                     KIGYKSKKARWTLHDAHINPGNSGGPMLNKYCEVVGVNTLVPTGTSGMSHALNIDILP
                     PLYFLVRDRTDEKYFCRPPENKK"
     gene            complement(26430..27659)
                     /locus_tag="P5056_00340"
     CDS             complement(26430..27659)
                     /locus_tag="P5056_00340"
                     /inference="COORDINATES: protein motif:HMM:NF020048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HRY62217.1"
                     /translation="MEKLAIKLPDSPGVYMFKKGRSIIYIGKATSLRDRVRSYFSRGV
                     IETRGQAIVNMVEQADGISVKKTDSVLEALILESTLIKKHQPKYNIREKDDKSYFYVV
                     VTSEDYPRIKTMRGRDLFAEDRKNFSEIFGPFPHGGELKEAIKIIRKIFPFREKCLPA
                     QAGLPGLGKACFEAQIGLCPGVCEGRVTKADYARNIKNIKLFFEGKKTKILNSLERMM
                     HSYANVHEFEKANEIKKQIFGLKHIEDVALIKNKEGLGDESTNRDVFRIEAYDVSHMG
                     GINRIGVMTVLENGEIKRSDYRKFKIKDPTKLAGDTNDLKQILDRRFFHEEWRLPDLI
                     VVDGAVAQKNAAERILEGLRLNIPVVAVTKDEYHRPKKILGKREIYGKYEREILIANS
                     EAHRFAVSYHKKIRKII"
     gene            27853..28404
                     /locus_tag="P5056_00345"
     CDS             27853..28404
                     /locus_tag="P5056_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62218.1"
                     /translation="MQKFTITVLLVSILTFCLATPVYAGGFFNRKTFQTIGLAVVVER
                     IAESEQNRNRRHDVANRNSNDLKRNRAEIEMKKERDQYARDKQMADLRFKQELERERE
                     LQKEERLHPASRYGDEGCELDDEFGVIILCDPKGRYKVDEYDEIFTSAKAVKQYVKSL
                     FPDRKVAMFWQKVADLETPLNTY"
BASE COUNT         7489 a         6857 c         5330 g         8824 t
ORIGIN      
        1 cgcgcacaga aatgacaacc cccgcaacct ttgatgtaac caactcattt ccgactcgtg
       61 cgaccggagc attctccccg acataatcac cttctttgac aaaattttcc attagaaccc
      121 cgttcgtttg cggagaaaga gaaacttcgt ctccgagtat ttgtgacttc tcaatataaa
      181 cccttccgga tgaaatctgc caataaattc caccgcccaa aacaccggcg accgttacga
      241 gaataagaag attcaatata ttctgatttt ttttcagact tttcaaatct tttttctcct
      301 gctcgagttc attaagtatt ttttcctcct ttcgcaaaat ctcttcctct tttttcatta
      361 actctttcgt atcttgtaac atgaatttaa aatattttaa taaacaagca cggtctcccc
      421 ctctgcgact ccggagacga tttcgacttt accgtccgtc ccccttgcac ccgcgacaac
      481 ctccctttcc acggcggcgc cttccttgcc gaccacaaaa acgagatttt tcatctccct
      541 actaaagacc gagcgctcgg gcacataaag aacattttga agagtctgac ccgcaatatt
      601 tatattggcg gtcatccccg ccctgatctt attgtcctca tttacaaact gcaatttcgc
      661 ccgataaacc ggcacgccgt ctacaacaga ctgagcggaa tcgatggaaa ttattcgagc
      721 gtcaaaaaag gtgttgttgt tataagcatc cagagtaacc ttggcggcat cccccacttt
      781 aacccttgat ataccggact ccggaatatt tgtttggata tcaaattgat ttgtggagac
      841 aatagaaata agcggaacgt taggaacggc aagttgccct acgacgacat cgtccttcgc
      901 tatctctccg ctaaatggtg ccctgatgat cgttttggca agctgagccc tcgcgctctc
      961 gagcgcggca ttcgcggagt caacttgacc ctcttgtgcg cgcagctgct ccggggtagc
     1021 tcccgccgcc atcattttgg tccccgcgct cgcaatatca taattcatct ttgccccctc
     1081 aagtttttca cgcgccgcgg ttatcgaaga gattgccgtg ttgatatttg acctaaccaa
     1141 cgttatgtcg gccctccacc cggcaagcgt cgtctgtgag agctgggagc ttgggctcaa
     1201 cgcattaatc acaagagcca aatcgtcgag aaaagattta atctgtctca agttagaaaa
     1261 agctttatcg cacggaccgg aaacatcgcc ggaagcgccg agtgacaatg tatcggaata
     1321 ccaatcgttc agcatagtct cgataaccat tcggtcaaat tcgagctttt ctttcttggc
     1381 aagatccggc acgacaaagg ttaatgccgg attcgccgat tttgggttgg agaagaactg
     1441 gtcaacctta cccctgatgg cgtcgtccga cttggtatat gcatcttgga tcttatctat
     1501 cgcattctgt ttagcttcaa caagggcact ctcggcaccc gctagcctag actgctgtat
     1561 caagacgtct tcatttctag caccccgttt taattcatcc agctttgcct gttggatctt
     1621 taggttcgcc tcggcttgat gcaaaagcgc cacaagatca taattctcga gagcaacaag
     1681 tgtcttgccg gcgctgactt tatctccgac attaaaatat ttagccgaca ccttgccgct
     1741 tctttcaaaa gccaaatcaa cgccgttctt cggcttcact tgtccggttg ttttgaaaac
     1801 attttcaatg tttcctctgg caaccacaaa gaccgaatta ccgttttgaa tgccgctatg
     1861 actcatcgca taaaacacga aggccacaag gccaaggaga actatcgttg cggctattat
     1921 cggctttttc atttgttgat tcatattatt cctttatttt agataatatt tttataagag
     1981 aaaccttgtc cttttgcccc agacacgaga acatattttt cagttctccg gaggccacct
     2041 taaaactgtc gctcagaaat tttttgccct taatcgtcac ctccattttc acaacccttc
     2101 tgtcctttcg tccccccaat ctcgtaagca ttccgctttt taccagtcca tccgttatcg
     2161 cggtcgccgt cggaggctta acacgcaaga agccggcaac ctctttcata tttgctccac
     2221 ccttagtctc aataaaattg aggacgtgca tttggccgta tgagaaagat cggccgatcg
     2281 aagctttctg gatccttgcc ttaacgacac ggctggtctc aaaaataacg ctcattaact
     2341 tttcgtctat ttccatattg tttagtatta aaaatattta ggttatctaa gtatatccaa
     2401 aaaaaaataa agtgcaagaa aatataaata aaaatgccga atagccggcc tgctcttctg
     2461 cgaggctcag gctgactcaa ttacgggttc cacaggcgct cgttaagtta atttttatag
     2521 ccgaagtctc ttaattcttt ctttgtcctt ttcaaaaact ttccgaaatt acgttctttt
     2581 tccctttttt caacatcatt catttcgtga tactcggctt cttttttgag attgatataa
     2641 tttgagtatt tttccttatc cagctcgccg gattttacaa ctttaataac ttcacacccc
     2701 ggttcatgcg tgtgtgtgca atcattgtat cggcatcttt gtgccgcgca agagatttca
     2761 tcaaaaaaag tatcaattcc ttggcccgca tccgccacgc caacttcgcg cattcccgga
     2821 ttatcaatta caattccgcc attttgaaga aaatacattt gcctgccggt cgtgacatgt
     2881 ttgccgcggt cggaatatga actaacatgt tcggtcttta tggcgccgtt accaagcaat
     2941 ttattgatca acgaagattt gccgaccccg gatgagccga ggaaacaata cgtcttgctt
     3001 ttagtgatat aactctgcaa ttcatctagg ccgtcatttt ttacaatact ggtcgagatt
     3061 atatcaacat cagggaacct gtttctgagc tctattattt tttcagccga ctcttctttt
     3121 gaaagtaaat cgactttgtt taatataatt gccggtttta ctcctccgtt ttctataata
     3181 gcgaaatatc tttcaaagcg gtttaaatta taatctcggt taaccgattc aactacgaag
     3241 gcaacatcaa tatttgtagc tataacctgg gttctgtctc tatattttct tttgatcatt
     3301 gaggccctgg gtaatatgcc gcggattaca gcggtatcct cacccatctc ggtaattgcg
     3361 acccaatcac caaccgcggg ataatcttct cttcccgagg cctcaaacat ctgcctgccg
     3421 gttattttag aaaaaaattc tccattcgca tttttaacca catatgcttc tttgtgttca
     3481 gagacaactc gggcaaccga aaattcagtc aagccaagct gatttctttc tgtttcaaaa
     3541 aatgatgtat agcctaaatc ttcaaggctt atatttttat ttttatccat tttaatcact
     3601 tgttttgacg ttccctggtc ggcatcgccc ctgtctccgc tatattataa caaaacaaat
     3661 ctttatgagc ggattttact aatttcgtat cagacccaac ttcttctttc tagttaggcg
     3721 cttcagctcc gcttcacggc ttctcgcctc gccaagagtg gcgaaagttt ccgtatattt
     3781 caataccacc ggccttcgta tcttcgtata atgtgcaccg ttctttgaat gattgtgttc
     3841 gtgtagtctt ttttcaaggg cgttcgtgct cccgacataa agtgttttgt ctgcgcactc
     3901 aagaatataa gttgtgggtt gctttggcat ggtttagttc ttaagacatg ttctgccgtc
     3961 tttatgaagc gtgacgggga attcgctaat attgatcttg ccgtctttta tatctagcgc
     4021 caaggtgaga ccggttgaga cttcctcgtt ccagtattga tcaaaaacaa aatttccaag
     4081 ggaataatac acggcggtat tgccgatatg ctcactagac aaaacaatgt gaggatgcga
     4141 gcctacaaca agatcggcgc cactttctgc aaatagtgtt gcgttgcctc tcagtctcgg
     4201 cgaagaatct atatattcgt ctccccagtg ggcatacacg atgactattt ttccattttg
     4261 tttttcgctt tttatgatct cggcgacttt ttcgggagat tgccctccaa attcattata
     4321 tgagacaaaa gaaatgtttt ctctgctata tattggctcg tcccctatta tgccgccaaa
     4381 ataattaaca cccgctatat ttaaaaattc ttttgttaaa gaaaatccac tcattccaaa
     4441 attgccgata tggttattgc ccaaattaac aacctctata ttgtgccttg caagcaactc
     4501 cgcgctcgtc gttggaaatg tgaaaacata attttcggga ctacctataa ccgtccccat
     4561 acttattgaa gtattttcgg taataggccc ctctaaattt ccgacaacaa aattataatc
     4621 ggataaaaat gggtctatgc aggaaaaaat aaaatctcct ccactcatat taccgacttt
     4681 tcttatatat ctatctagct gaatatctcc tactatcaat atttttgtgg attccggggc
     4741 gggcaacact tcgggagtat ttacaacggg agtaacggcc ccaacctctt cggtgatgat
     4801 tatggcttct tctttctgcg ccaccacagg tgctggaacc gccttctgat tcctaaaata
     4861 cgagttataa aaaatcgccc caataaggac aaaaacaatc ccgattttaa aataaatacg
     4921 ctccacgagg taattatact acaaatacaa aacctcgatg ctaacgcgcc gagggtttta
     4981 tgtttttact gaccgtttca attattcgag aaggtggcag actttacaaa gaatcgaaga
     5041 tgtaggttgt gcacagcact aaccctatat tctatttttc cgtaagtctt ttttctactt
     5101 cttttataag aaatagataa tattgcctat tcggtggtat tccaaaatgg gcacgcctac
     5161 cttcataaaa aatacacgac attaaatcag ctgtcgaata tttaataaat tttttcttaa
     5221 tcttatattc tttgaccatc ctgttcgcaa ggtatccgca acgctttata ccaccacaat
     5281 aattgtagcc gttgaatgta aatatcgcct tacgaatctc gccacgcttt gttctatttg
     5341 tattatatct tcccatcttc ggcccccact cccaatgaca gtggccacaa tgccacttag
     5401 cccgcccaga attcatttct ccgataattt tggaacaatc agagatgtgt ccgggatttc
     5461 catatacaat ctcctttatc gcatcatttt tgcactttct acattgcttt ggaattttca
     5521 tgaaatttta ttttccattt tctccaactt tcccagcgtc caaacgataa aatctagtat
     5581 tttaatgtcc gacacttgat acttattaac gccaaattct tttctgaaaa tagaaattag
     5641 ctctttaatt ttatcatctg aaagaagttt taaatacaaa ctttttagaa aattatacaa
     5701 ctctacgcag gattgaattt tttgctcttt attatttccc gctactcgcc tctgaattac
     5761 atttgatact tcggcgtcaa aaattggttc attattgtct attgtatgaa ttaattttgt
     5821 cgcaaaacta aattggatgg tgttattgcc tcgtagggtc ggtaattggt atagggcgtc
     5881 taatattttt tccaagtccc ttatcttatt agtcattaaa tcgaaaaagg ttcttttttg
     5941 attttgactt aaacccgcac cgtctagtct ataataagat cgaaatacaa attgaaaaac
     6001 acgattgtgg tcaataccgc cactattata tgccgtattt aaccattgat aagtgtcgac
     6061 actctctttg tctatatttg agaatatttt ttcttggttg ttataaattt tctcaattat
     6121 tgtgtctggt gtgtttctcg ctttcataaa ttttcattat ctactgcggg acttggaatc
     6181 gaaccaagat actgagcttc aaaggctcat gtcctacttc accccgccaa attttccggt
     6241 caaagctgcg ggacttggaa tcgaaccaag atactgagct tcaaaggctc atgtcctacc
     6301 gttagacgat cccgcagctt tgacaagaaa ctttctgaaa gcatttccgc cgtgccagga
     6361 ctttatttgt ctctcccgca gacgtgcttc cttcatattc tcaaactctt caacatatat
     6421 taacttccac ggccgtttat attttgtaga aaatacgtca cccttattat gtctaattaa
     6481 tcttttttct ggctccgact ccgtaatacc aacataatga ctttttgccg tattttccaa
     6541 aatataaaga aaggctttca tcaaaatttg ggcggggcaa gttagacgat cccgcaatga
     6601 tattgtcaac aataaagaac tatggatccc cgatcgagcc gaggatgaca ccagttacca
     6661 actggattgc cgcgctacgc tcgcaatgac gaccaaaaaa cttgataact ttataacatt
     6721 aaaacgttac aaactcaatt ggtttgaccc aaaaggcgaa ttaaggcaag ttcgattggg
     6781 agttccggta tgcttccgtg gcctagttcg tgattggcat aaataagctc ctttaacgcc
     6841 tgcggcaaaa cagcaatatt tttgtggccc tttaattcct taaatacctt tgcctcgtca
     6901 tcagaaactt cagacaatat ttctttttcc atttcggggg cgaatttgag aagcatcacc
     6961 aagcgaatat cgcgaagaag cattttcaaa aatatcttca tgtcgatatt tttgattgca
     7021 atcttgcgaa gaagaacgag ggcggcctca agattctttt ctaatatctc ttttataaga
     7081 gaatgaatga cttcgccgcg aggagagccg gtaattgact caatgtcgtc tatggttatc
     7141 ttcttgtcct ttgaaatatt tgaaacctgc tcgaaaagac ctatcgtgtc tcggaaagag
     7201 ccgtcgccaa gaagcgccag caggtccgcg gagcttttgt ctattgttag cccctcgcct
     7261 ttcgccactc tttctacagc ctttttcaat atctcatttg aaggtttttt gaatgtgaac
     7321 gactgacagc gagacacgat cgtgtcaggc accttgtgag cttcggtagt agccaagatg
     7381 aacataacat gcgcaggcgg ctcctcgaga gtcttcaaga aggcattaaa agcgctcttt
     7441 gaaagcatgt gaacctcgtc gatgatataa accttgcgtt ttgattcgaa cgggagcgtc
     7501 ctcgcgccgt ctgtgatctc tcttatatct tcaacgcttg tatttgaagc cgcatcaagc
     7561 tcataaatat ctttttccga cgtgccaatc tctttcgcaa aaattctcgc gatacttgtt
     7621 ttacccgttc ctcgtggccc gataaaaaga tatgcgtgga atattctatc ctcgtctata
     7681 gagccggaaa gagcgcggac gatattatct tgcccgagga cctctttaaa attctttgga
     7741 cgatattttc tatatagtgc gatgctcatg tgagattagt tataaggtta taatgttaaa
     7801 agttttaaag tctaaactat aaaaccttaa taaacccgga aatcaacttg gatgcgtcaa
     7861 tagaaacctg cttaaaattg tcgtgttctt tcttcgtaaa gtaaccaagg tctaacccta
     7921 agtctaacat ggagcgcacc tcaccacacg agccctttgc gatatacaga aaacttataa
     7981 actctttatt ggtatgacgt tcaaaacctt ctgctatatt attcattacc gacacagccg
     8041 ctcgctgtat ttggtctctg aaactatagt ctcggcagtc tttgagagtt ttataaatac
     8101 tcaaggtgag ttcctttgat ttctgccaag ccagaatgtc ctcgaatttt tctattttca
     8161 tcgaaacttt ataacattat aactttcaac tttataacta accttaaatt cgttccggcg
     8221 cctcggcatg acacaacttc agaccgtttg ccattgctgt cgctaccgcg ctagaaatga
     8281 gaaggcggtg cggatataca agcttgcctg attccaatac atcaaaattc tcgtaatagc
     8341 tattgtagtc gttcgcaata tgcagtaggt gctcggcgag cgtggcggga ttgtctgtct
     8401 cggcggcttt cctcaccact atcggcaatt cggcgacctt cttgattaag ctgatctcaa
     8461 cattttcaag cttagagaaa tctaatgtat ctatatttat atcttcaact tttttgtctg
     8521 actttctcaa tatgcttctc gcacgcgcaa tggaatacat tatatatgct ccacccgact
     8581 cttcgaaatt gcgaatattt ttgtcgagct ctttatgaaa agcgattggg aattttttgt
     8641 cctgttttat gtcattgaaa gcaattgagc cgactgcgag cagttttgaa ttcttttctt
     8701 tttcttcatc agtaaaattc tgcgcctttt cgccggtgct cctttcctcg tatttgttcc
     8761 tgaaatattt gattgattct tccacaagcg aaaccacgtt ctgggcaccc tcacggctcg
     8821 aaagcttctg cccggtcgcc gcatctacat aaaatccgtg gtaaacgtgt tctagaccaa
     8881 tatttgcccc tttgtttaaa ttaagcgtca aaacggcctc aaaaaggtgc ttaaaatgct
     8941 ctgtttgctc ctgacccacc tcatagacca gtcgggatgg aatgaacgtt tctaggcggt
     9001 gttttgcgct tacaaggtct cttgtggcgt aaatagtggc tccatcagcc ctcataatga
     9061 ccattctaga ccccgaagga agcataatta catatgcgcc aatatcgtga cttatttcct
     9121 caagaagctt ctcgagcaca gccttcttta tcgttcctgc ttcaaatgcc aactgtgctt
     9181 tctttatctc gacatccgcc aattcttgcg tgaactgcac agcaccgccg gtttttaaaa
     9241 gttctttgac gagatccgcc ccgctcttcg cataaaaact ttcgccgagc agatagtcgt
     9301 gcttaatatt taaaatttca taaaacttat tgaattcaga gagactccat tcgcgcatct
     9361 tctgccagag agcaaactca tccttgccgc catcctccaa ttttctaaat cgctccttcc
     9421 cggcctctac aaattctaca tatttatttt tgtattccgc gaagtcggca aaatctccta
     9481 atagtttttt tattacatca ttctggtccg tgcccaacaa aaacttatca aattcacttt
     9541 tgttggtaga catcttttct gccgttctca cgattaaata aacttgatag agcatatcat
     9601 tgcccgatag gtctagtgac caagcatttt tatctttcaa ccttaaatac gcctccatta
     9661 tcgcgcccat tcctccccag tcgttaagat agtttaacct gtgaaccgta taaccggtcg
     9721 cttcatatat ctttgccaaa acttcaccga ttattgtcga gcggacatgc cccgcatgca
     9781 aatgctttgc catattcgga cttgaataat ccagaacaat gctttctccc ctgtgcttgt
     9841 cggaatatcc atatctatca ccaagcacga gcacattgtt tataaggcaa gagaaacatc
     9901 ccggcttcat aaggagattt atataaatac cggttggggt tattttttct atatgctcct
     9961 tcacgaaact agaccgctct aagtttgaaa ttatctgcgg aatgtattct ttggtatatg
    10021 ttggaatccc ataagtcttc attaggtgcg gaacctttat tgcgatatct gcattaaatt
    10081 tttcacgatc aataaattcg atttcaacag tatcaaaacc ctcaacgtct ttgtttgtgt
    10141 ctctcaactg ttttttgaaa tcttcaaaaa catattcggc gagcggaaca tatcctgcgg
    10201 cttcgcgctt cttaataacc tcgtcttttt tcttgtttac ttgctcggaa attgagctgt
    10261 tccacacctc gagcagattt tgttgcatag aaagaattat actaaaaaaa taatgtaaat
    10321 cttacttgac acagagatca tttgtgatag tttgtattta aaattagttc ttcgacaaga
    10381 tcaaggggcc cttaacgata acggccaaag gccaccgggt cactagcttg ctagtgaaag
    10441 agaggtactc agagatggaa actcccctga tgctccttgg catgtttgtg gtaatcctgc
    10501 ttatcactct aatcacaact gttatcgtag ggcttttccc aagggcaacg gctcggctga
    10561 tgggcctctg tgtatatctt ggcctcctat ataaactgat tcccgcgatg aattctgcgg
    10621 taccaaaaga agcgccgcta cttcacatta tcttggcaat catcatattg tttgaggcga
    10681 cgggtatatt cgtattgatg gcaaggttta tcatgctcgg agccatgaga gactatagaa
    10741 acatgtacta caatattaag gaaagtcagg aggacatgtc tctttcttag tttgctcacc
    10801 actcttttaa aatcacccct cttcggaggg gttttatttt attcaatatg ccggtcattt
    10861 cgactttatg gagaaatccc tatagagtaa attcacggaa taacaagcga ttcctccaca
    10921 aggtcggaat gacagcactt gtgcgtcgca aataatttaa ttcgtaattt ataattcgaa
    10981 atttttaatt actttctcta cttcatctgc gcattcggtt gtcatcaatt ttgcgcggag
    11041 ttcgcttgcg ccggggaaac cgccgacata tgagccgaaa tgtttgcgca taagaacgaa
    11101 cggcttcttg ccacccagaa gctcttcaaa aagtctcgca tgttctagca ttacttttaa
    11161 tatttcagaa agaggcaaat cgctcttttt tattttttca ttgaagaacc acggattccc
    11221 aaatattccc cttccaacca taattccgtc tacgcccgat tcatctctcc tctttctccc
    11281 ctcttctaga cttttaacat cgccatttcc gataattacc gtatcagtat ccttggccaa
    11341 ttccacacac ttcgcgacct cgtcccagtg tgcggaaacc tttgacatct ccttccttgt
    11401 cctcccgtga atagtgagcg cggcaggctt ggctgaaagt attgccggta tccatgtgtc
    11461 tatttcgttt ttattgaaac caattcgtgt ttttacagaa acaggaatat ctcccgcgcc
    11521 ttcttttgtc gcttttatta tctcctgcgc aagcttcggt gattttatta gattcgctcc
    11581 ggcgttttgt ttatttattc cgtggtcagg gcaccccata tttatgtcga ttccatcaaa
    11641 tcctagttct cggacatatt ttgcagtttc gcgaaaagtt tccggatctc cgccgaatat
    11701 ctgcgccacg atcggcctct ctgattcatc gtaaatgaaa ctaagagcca agcggtctct
    11761 ggcaggatat ttggagccgc gcggtttttg ctcggccttc tcctcgtgca taaggccgtc
    11821 acaagatacc atttcagtcc acattacatc gggcttgccg tatttggaga acatttttcg
    11881 gaacggcgca tcagtaacat ccgccatcgg cgcgatacag aaaaagggcc tttttgtaag
    11941 ttttagtttt tgccagaaat tcttgttttt catgattatc ttgagtatat tagtagaata
    12001 aaggctcgtt gacaacttcg ccctcctaaa tttttcatca gactattgta gtcttggaag
    12061 aaacaataac gaatataata aatatttatc tcgtttaaat ttaactataa aattttggcg
    12121 ggcaaacaaa ttcgacatta aaaaatctct cgaaaggagg aatacgtttt gggtaaacgc
    12181 agtaaggaga aacgaaagaa acaacgtaga aaagatgaaa ttgaggaatc gtgcactatt
    12241 ccgatcgaga agaagcctcc tgaggccttc cgcttcaacc acatggaatt tgctaccgcg
    12301 gacgaggcag cgaccgcgaa attcatcttt aggaacaaca ttgaaaagga cgagcaagaa
    12361 ctgcgaaagc ttgtcgccct tatcatcaaa cacaatattc ttagaaataa cgaaacggag
    12421 atcagattcg cacgacttct aatgaagcac aatattcgtt ttgaatattc gaaggaaatt
    12481 gaaactcaaa agattattca aaaagaagaa tattcggaaa ccccagagac aaccgcgaaa
    12541 gatggcgaga cgccctcgaa acccggcgtg cgccaaatcg atttctggct acccgacgga
    12601 ccaatcatgg ttccgttttg tcatgaaccg gtccagtctt ttgaagtaaa aggcggcaag
    12661 ctaactatga attgttggcg ccaacggaga gagctccgcg atgccaacta ttttactttt
    12721 attgtgctac ctcaatacat ttctttttgg gaaaaacatg gtctgctcga gccggtatcc
    12781 acgagagaaa acaacgctcg cgggatctat caaaaaccgg cctgcctaat ggcaaaccat
    12841 attccgaaaa aagaaagctc cctcaagaaa aagatggtat cagtgctcag gaccgagcac
    12901 atcaaatttg aatatgcgga agatgtgcgc ggcgagcgac tcggagacgg agcgcatagg
    12961 gccatcttct tcctgaaaga acctgttaag ggtttctggt gcggagaaat gacccaagca
    13021 atcatgctca ttgagggaga tatcacaatg aatgattttg agcgctggca agaggtatct
    13081 gaaacgcaga acaccttcct tgcagttaag caattcgtga ggttctggca tggtgtcgga
    13141 tttcttagac aaaaagcaaa atagtttagg cccctttatt aggggccttt tttgatatat
    13201 agttattgaa tggaaacctt gcttaagctt gcagaatttg cgcttcatat agacaaatat
    13261 cttgtgcaaa tagtcgccaa ctacggctct gaaacttacc ttatactatt tttaacaata
    13321 tttctggaaa caggtatggt gtttacacct ttcctccccg gagattcgtt gctttttgct
    13381 tcgggcatga taaccgcaaa tagcgactta aatatctttt ttttactaat aatacttagc
    13441 gttgcggcca ttctcggaga tagcgtaaat tattctatag gaaaatattt cagctacaga
    13501 ataaagaacg ggcaaaatat cccgttcgtt aaacaggaat atgtagaaaa aacagaacga
    13561 ttttatgaaa aatacgggaa caaaaccatt tttatcgcgc gcttcgtgcc ggtcataaga
    13621 accatggcgc cgttctttgc aggttttgga agcttaaatt attctagttt tattttttac
    13681 aatattgcgg gaggcttgtc gtgggtagcg ctatttctct tcggcggata ctattttgga
    13741 aatgtgcaga tcgtaaaaga caacttctcc gccgcgataa tgttcgtaat tattgtgtct
    13801 atcattccgg caattgttga atgtattaga gaatcgcgat tagggaaaaa ataggcatct
    13861 cgccacaaaa gacaaaaaca cctcgcagat tgcgaggcgt ttttaaagtt atctcttgct
    13921 ccagtgagga atgctccgca tttagaaacc cgcggtttct aacgcttcgc tgttttcctt
    13981 cacgggaaaa atagagtttt cccgacccct ttcctactcc gcagcgacaa aaagaaaaaa
    14041 ttcccgcttg gtagcgggaa tcttttatta tctttttgac cacttgtgga tgctccgcat
    14101 tcaaggaccc acagtcctcg acgggcaaag ccctgctctc ctacacaagg ggaattcttc
    14161 ccctcgacca ccctttcgcc acatttccgc aagagataaa aatgcctcgc aatgtgcgag
    14221 gcattttaaa tttatctctt gctccactgt ggcgcctttc gagctttctt caatccgaac
    14281 ttccttcttt cctttactct cggatctctc tttaggtgtc cttctttctt gagaggcttt
    14341 ctcatctcgg cgtccatctt tgtgatggct cttgcgatac caagaagagc ggcgtctacc
    14401 tgtgagctca ttcctcctcc gttaaccttg atagaaacct tgagcttgtc tttgagatct
    14461 gctctgttta cgaggtcggt aatcttctgt ctgctgtcgg cggtattcca gtattccttt
    14521 atgtccttct catttaccac aaccgtgctc tttgtgccgg aagcgggcca gaccctaact
    14581 ctggcaacgg agcactttcg tctcccaacg gcttcgaaat atttttctgt tgtttttgcc
    14641 ataaatttta ttcgctaacc gttaaatttt tgatcattac agccctcaac ttgttctttg
    14701 ggagcattcc gtaaacggcc ctcttgaaaa cttctccata accgaatttt gcgacaaccc
    14761 tctccattgt ttcggacttc tgtcctccgg gatactgaga gtgtctcaaa tattcttttc
    14821 ccttcatttt tgcggcaggg attttggcct ttcctgtatt tataatttta acagtaactt
    14881 ttggagcaat gtttgattca tactcgggag aatttttgcc catgagcaac atcgcagcct
    14941 cggaagcaac ccttcctact gttctgttga tagcatctat tgtgtattct ttctttgcca
    15001 tataattata caaattctac tatggccatt ttgcttccgt ctccgcctct ctggcctaat
    15061 tttacaattc tcaagtagcc tccctttcga tctttatatt taggggctat ttctttaaaa
    15121 atcttagcgg tttgttttgg agacaccaag tactggctta caagtcttct tgtggcgaca
    15181 ttatcttcct tcgcctttgt aacaagcttt tcaacgaaag gtcttatctc tttcgccttg
    15241 gcctctgttg tgatcatctt ttcgtagttg atcaaattgc aggctaaaga tcggagaagt
    15301 gctgttctct ggtctgtctc tctgccaaat tttctattgg tattgtggtg tctcataaaa
    15361 ttttacttac tttagggtta tcccaagatt acccaaaact ttctttattt catttatacc
    15421 cttttcgccg agtcctgcga tctcaagaag atcatcttct ttctttctgg cgagtccgcc
    15481 gactgttcta atgcttgcct cagacaacgc tttcattgtt cttccggaaa gcttgaggtc
    15541 ctcaattctc atctttagaa cctcttcctg attttcagcc tttacctcgg gagctgcggc
    15601 cttctcttcc gccacttcct ccttaacttc ctcaaccttc tttacctctt cttttgtttc
    15661 ctcttcatcc acgaaaccga taacagactt gagctgggaa atcattatct ctattgatct
    15721 ctcaagagcc tgtcttggtg taagtgttcc atctgtctca ataaataatc gaagtctgtt
    15781 gtaatctgtt ctgtctccga ctctcatatt ttcaacttcg tagttgaccc ttcttattgg
    15841 agagaaagcc gcatcaactg caatcgtgcc gatctcaacc ttatccttat tgataagctc
    15901 ctttgggacg aagccgaaac ctttttcaac agtcaattca atatttaatt ttgtgttctt
    15961 gtctgtgata gttgcgatgt gctgttcggg atttagtatc tttacagaag ccgtctcctc
    16021 tatatcggcg gcggtaacat ctccgacttt tgaagccttg agatatacaa cctgaggctc
    16081 atctttgtct actttgaacc tgagtttctt aaggtgcaaa actattgcta taacatcctc
    16141 cttgataccc tctaaggtag aaaattcatg gtcggctccc tctattttaa cggaagtaat
    16201 tgtagcgccc ggaagagagg acaaaattat ccttcttagg gagttaccta aagtgtgacc
    16261 gtatccggga tacaatccgt ctatctcata cacgcccttc aactccgtct cggaaaagac
    16321 tctcggcttt gatggtagaa caatattgaa atccaacata ttattttaaa attaaactaa
    16381 attatttaaa aactatgttc tactataaaa ctccataact acgtttaggt tcaaagtctg
    16441 atctccgtcg gcaagctccg gaaggtatat aacctccgca accgacttat ctaagtcaaa
    16501 cttcaaccac tttgggaatt taaaatcctt taatttagat ttaagttctg caaagagagg
    16561 gctcttctgg ctggactgtc taacggaaat tttgtctccg agcttaactc cataagaagg
    16621 agtgtttaat ctctttccgt tcaccaaaat gtgtccgtgc gagaccatct gtcttgcaaa
    16681 tgctctggat gacgcgaaac cgagtctgaa aataacgtta tcgagtcttg tctcgagagc
    16741 ctggcaaagc atgtttgcag gattgccctt ttttgaagat ttagaggcat taacgtagtt
    16801 cacaaactgt ctttctgtaa ctccgtatgt aaatttggcc ttctgcttct cctggaactg
    16861 tgtgccatat tctgattttg gctttggtct gccctttcgg ccgcctctag caccctcctt
    16921 tgtaagggta gatgtatacc ttgggctctg gcattgtgga aaaaccattc cgccgaccct
    16981 tctacatact ttatactttg gtcctcttaa cattacttta tattgaaaga attaattata
    17041 ctcttcttgg tttctttggc tttggtccgt tgtgtggcac aggagtcttg tctgtaatgg
    17101 ataagatccc tatcccctta gaagagaaag ctctaattga ggactctctt cccgcaccta
    17161 ctccgctaac gaccacgtca acctcgttaa ctcccatagc cttagccttg tctccgagta
    17221 actcgccgac tttagccgca gcaaaaggag tgcctttttt agcacccttg aaacctaatg
    17281 taccggaaga agaagcggca acagcgttac ccttgctgtc tgccaacaaa accttggtgt
    17341 tattgtatgt tgcttgcacg aagaggatac ctgtagacat tttgtgtcta ggcacctttg
    17401 gagccgcggc ctttgtatcc ttgatttctg cgttttcgtt tttaactgtt tttgtatctg
    17461 tagacatttt tgaccaatta gttttaccct cctaaatttt ttaactgttt aatatttttc
    17521 ttaaaattat attcgtctct gtgaagttaa cttttatcat ctaattttct ttgttaagaa
    17581 aattttggcg ggttaactac ttcttctcaa ccttctttct tcccgaaccc atcgtctttc
    17641 ttgtgtttcc tcttcttgtt ctggagttgg tcttcgttct ctgtcccctt gatggcaagc
    17701 tcttcatgtg tctgaggcct ctgtagcatt ttatatcctt gagtctcttg atatttccgg
    17761 ccacctctct ttttaattct ccctcgattg tcctgctttc aacttctttt ctgagagcat
    17821 tctctttctc ttcggaaaga tctcccggtc tagcgcccct atctattcct agcttgtcta
    17881 aaattgactg agcaatcggt ctgcctattc cataaagcgt agttagacca acctccaatc
    17941 ttttattatc tggtattgtt aaacctgaga ttctcatgaa aagtttttat aattcttaat
    18001 taaaattaac cctgtcgctg tttatgcttt gcaatttcgc aaataacata aacacggccc
    18061 tttcttctga ccgatttaca cttcgcacac attttttttg ttgaggatct tattttcatc
    18121 gtttacattc ttcttactat tctccctctc tctccgtgtg aatccaactg gactcttact
    18181 ttatcgccga tcaatatttt tatcctattg atccgcattt ttccggaaag atacgtgagt
    18241 attaccttat cgttgccaat atcaactcga aacattgtat taggcaaaga ctccacgact
    18301 acgccctcga cctccgctcc cgtcgatgag ctgtttgtaa cttttatatc cattttttaa
    18361 cgtcagagta tgatagcaga aaagagccca ataagtcaac cccatagtag attttggcga
    18421 tggccgatgg ccgcgctatg tgcgttgcta aaattaacta cggggtcaac ccccttatct
    18481 accgccaata aatccttatt ttacttgata atacttaatt gcctcgttat ggtgtggggg
    18541 cttgtcccgc aaccatcttt caaaattagt cttctattaa ctcgaccgtt atcctgccca
    18601 cctcatcagg gaaactcccg gaccaagacg ggttaaatat tgtttcagct ttttctatcg
    18661 tcggatattt tttaaaaatg tcctgataat tctctttttt gatgcccaat aggtctttct
    18721 tgaaattatc tacgtccaag ttccaggtga cctttgcgtc tccgttcaat ttgaaagtaa
    18781 acattttact tgaatccgga gaaacaccct ccttgaccaa aatcttaaac tcaagggcgg
    18841 gaagttcatt cgaatgaaca tcataattat ttaaggaagg aagttttttc tttgtcattg
    18901 cggtagcaag ctctttcata tcgaacataa acgcgccggc ggaccccttc attttgagat
    18961 acaccttgtc cccctcccat tctgagccaa gttgttttat gttatcttca aaatcaacga
    19021 acatcgcatc ctcgtaaaca atatatccgg ccggaacttc cgacgcgatt ttttctctca
    19081 atttcttcgt caaagaatct tcaagcttct tttttgtgag agcaacatct gtcggagaca
    19141 ctattttcat ctcaccgaca taaccgccgg acatatcccc cttgccccta ccgtatacgc
    19201 ttgtgtatct tggactgccc ttaagacccg gcaaggtata atctgatgcc tttatattgt
    19261 attccttacc cgcctcggcg gcataaaccg taactgttag cgaccccggc accaacttgc
    19321 cggaaaccgt ctgcttgccc gggattgtaa ccgccttgtc tattttgaat attttgccgt
    19381 cagaagattc aaaccttgtg cccgctacca acttttggga ggcggtgctg taattattgt
    19441 aaacaactat ttcaccgctc gctttttgtg atacggcctt tgattcagtg gcggctatcg
    19501 ggagagaatc attctcctcc actttcatgg tcttgaaaac aacgccgtct ttcaccgcgt
    19561 ctttaacaga aacagcaacg aaagcgtcat ccatagaaac gttttctgag attggggtta
    19621 ccttaacgac cacgcttgaa taagccgacg agaaccaaat aaacgcgagc aagagggctg
    19681 ttgaaacgga gatgatggtc tttgtgcttg tagcactaaa aaatcttttc cccgatttct
    19741 tgtctagaga aaaactcacg ggggttcgcc gtctgggcac ctcttcataa agatccttat
    19801 gcacaggacg ctccgcccta ctgcccgctc cggaaataaa atctttgaat ggttgttttc
    19861 tcattggatt ttaaaaatta ataattttta cactacaaaa tatacctctt ctcttctccc
    19921 caacgatgcg caatattctg ccggtaaaac ttgtctataa aaatcgcatc aacaagcaag
    19981 tgaagctcgt ccgggcagct gctgatattc ttacaaagtt cggcgaataa atttggctca
    20041 agacactcaa cccgcggcga gcggcttgtc accagcaatc ccgaaacctc cgccttttga
    20101 ataaaattaa taaaaacatc tttacacgtc tcttcggtgg cgatataaaa aatatccggc
    20161 aaaagacgat ggaatgatat ttttcttaag gtttcataaa aatggtcggt ccacaagacc
    20221 atagccctgt ctatcgcatc caccattttt acggacaggt ctttctcggc agatccggac
    20281 aaatataatt ttagataaga aagtgcttgc tcgttagaaa gattgaatct atccattata
    20341 accctcgtga gcatgctcat accaacaggg aaagatgcga tatcctccac ttcgccgtcc
    20401 cttattactg aaacttccga tatttcatcc tctatgttta cgacgacata actaggtctt
    20461 tccggggcta tttttcttac aatatccgac agcgcaaaag taaaagaatg aaaatctatt
    20521 ttgttggtat gcaaattctt aaaaatattt tttttaagac tatccaacaa ctcacccgga
    20581 gccatgctca caaaaaccga aatctctatt tcagatgcgt tcttttttat tggtgagcta
    20641 gtttcatacc cgttcaattt aatttgagtt atcttgtgtt ccatcataac gttgggggaa
    20701 tcagatgcat ttgtatttaa cagatgcttt tgttgaaact cttttgaatc ttgaagtatc
    20761 aaagagtgaa gaacttcttc ggaaatagta acgggtttgt catatttact cttaattatt
    20821 ctaacctgag aagcgtggag gtgaggacca agtatacaaa atacttttga cggtatctta
    20881 aaaccggaat cccttatcgc cagagcgacg aacgctacgg cctgagagat tgaggcgagt
    20941 aagcgcttat tattgaaact tctttggaag ggtatttctt ttctaacact aaaaactttt
    21001 atcggagcac ccccgccctc tttgttcacg gtcaatatca tccccccgac agatccgtcg
    21061 cgtatatcca acacaacaac gtcctccttt tttataaaaa caaaagggtt tatcacttct
    21121 aaattataac attcttttgc cgcaagttct ccagcccgct tgtggaaaag aaaaagggca
    21181 gaccgtagtc cgcccaaaac aattattctt taacgcaaga gaccgtgccg cggtccgaat
    21241 acttattatc ggggctctcc gcatcaacgc ggatcttgtc gccctttata taaaccaccg
    21301 gggtcttccc attgcaacga gagccggcaa accacatgcc gtttctcgcg gcattgcccc
    21361 cgagaaagaa cataaaggtg ccctcaatgg cattaagagg tctgcgcccg agtgaagcga
    21421 gttttttggc ggcttccttg ggtgtcagcc cgagcatatc gccgccggaa gaaacatcaa
    21481 acacataata tggtccggaa tgagagttgc tctcccctaa aagcggataa aattgcttaa
    21541 agttaacaat gtccaacttt ctatcagttt ccttcagctt gggcctaccg catctgcccg
    21601 tgagaccata accgacgcta attgcgggca caaaaggaat atttcccggc gctatgtcca
    21661 tcttggccgc cttaacggta accgaagttc tctggaccat aagatcaccg agaaactcat
    21721 cgccaaaacc tcgtgcacga agctcagcga tctgattatc aaaaatcttt tccatcctcg
    21781 gaacgttgtc catgaaatta gcaacgtgga ttgtttcgat gccaaaaaca agtgcagggg
    21841 aaaatgaaat aatgagcgcg gaaataaata atgtctgaac aagaatacta ttttttctca
    21901 caaaaattcc tccccttccg tgtatttttt tgcgttaaaa atatcaaaga acctaaagac
    21961 ctatctttag gttatcttat taccggaaaa aaatctactc caggacggcc tttatccttc
    22021 ttacgccggc cgcaacagcc tcttctttgg caatcttaaa gtggtccaat tcgctcgtgt
    22081 tctttacgtg aggacctccg cagatctctt tgctaaaata attatttttt tcgccgatgg
    22141 tatatacggt cacgacatcc ggatattttt caccgaagat gcccgtcgct ccttcttcgc
    22201 gagccttatc caaaggcatc tcttccacac ttacagggag cgcttccttt atcttctgat
    22261 ttaccaggtc ttctactttc tttttttctt catcggtcac tttagcggga tttgggaaat
    22321 caaaacgaag tcgctctact gttatattgc tccccttctg atacacatgt tctcccaaaa
    22381 cttttttgag cgccgcattc aaaaggtgtg tagcggtatg atactttgtc tcaatttcag
    22441 aaagcccgcc tagtccccct ttgaacttct tctctgagcc ggcgcgcgac aattcctgat
    22501 gttttctaaa ctcttcctca aaaccttttt tatcaacttt tacccctttc tctttagcta
    22561 attcagctgt tatctcaaac ggaaatccgt gtgaagaaaa tagcacgaac gcatcagcac
    22621 cggagatatc cttcttgctt attttttcga acatcttcat acccgaagcc agagtctccc
    22681 taaatcttgt ctcctctttc tgtatctcgg aagcgattgc aatcaggttt gtatttatat
    22741 tgtcataaac ccccttatat ttttcggcaa caagcgaagc aaaagtagcc aaagccccct
    22801 ctttcattcc gagcttgtct gcgtgtctca ctgcgcgcct caaaaatctt cgcaaaatat
    22861 atcccctctc agagtttgac ggaacaacgc cgtctgcaat catcataacc gaagaacgaa
    22921 catggtccgc cacaattctc atagaaacct tgtcggctcc ttcataattt ttgcaggaaa
    22981 caatcttaat ggcctgaata atgtcaaaaa taagatctat ctcaaaaata tcaggattat
    23041 cattagaagc ggcaacaatt ctttcaagtc cgcctccaaa atcaacgttt atctgtggaa
    23101 gttttttaaa tgagccgccc gcctgtccgg taggcgggtc atcctgtttt tggaattcca
    23161 taaagacgga atttcctatt tccatgaagc gaccacaatc acaatttgga tgacaatact
    23221 ttccaaattt tggattgtgc tctatgtgta taaattcata aaatatctca gaatcagggc
    23281 cgccgggctc acccacaggc atattcattg gaacgccgga cctactccac cagttctttt
    23341 tggctccata atagaaaatt cttcctcctt gcatgccgag ctcaccgccc ctctcctcgg
    23401 taataagctc aacgtcttta gcatctattc cttttttctt aaaaagctcc ttccatatct
    23461 tgaccgactc ctcgtctctc tcgactccgg cgtctttgtc tccggcaaat acggtcacat
    23521 atagtttgtt cgggtctatt ccaacctcat ccgtcaaaaa ctcaaatacc cacggcagtt
    23581 gttccttttt gaaataatct ccaagagacc aattccccaa catttcaaaa aaagttgtgt
    23641 gtctgttgtc cccaacatcg tctatgtctt cggccctgaa tgatttttga gagtttgcga
    23701 gccgaacacc catcggatgt ttctctccga gaagataagg cactagaggc tgcattcctg
    23761 aactcgtaaa aagcgttgtc gggtcatttt caggcaccaa agaagccgac gcgataatcg
    23821 catgttcctt ttttctataa aactctaaaa attttgcccg aacttctttt gatgtcataa
    23881 aattaaattt aacgcactac ggggtggacc taggcggaat cgaaccgccg cctgtacaat
    23941 gcgaatgtac cgttttacca ctaaactata ggcccgtaga ccgacaatac actttactat
    24001 ttatttacca ccgtcgcaag agaaaaaata ccttattttt tgaccccgta gtgaattttg
    24061 gcaacgggcc attgaaaatg gcccacgcgc catagcgcat tgccaaaatc tactacaggg
    24121 ttgacgccta cgacaattat agtatctggt attgttgcgc tttgctgctt cttattgcgt
    24181 gatacaatta tattgaggtt attgtccctt gaaaaatcta taagaaagga ggaagtttac
    24241 catgaagaag attcccctca ttgcccaaat tgttccgttt cttctcgttc ctattatttg
    24301 cactctgatt ctcatgttca ctttttgcgc ccacatgacc ggtgaccaga tttttggctt
    24361 tgccaacaat ggcgcctggg tctacgggcg ttgtcataat tccgttggcc aagtttttgc
    24421 ggttcacacg attcgcttca aaaacccggt cgttcaagcc gttatgcaaa tatttttagg
    24481 ggtcccgcaa gtgaccatct acgggtccgg tttctacttc gacaaagact acccctatgc
    24541 ggccactaat agccacgtcg tcgccgctcc caaaatggaa tcggagactt tcgcaagaat
    24601 aatgacgggt atgcggaagg gctaccgcgt ctataccgac aatagctgca caaaagcggt
    24661 tgttggcggc caaattattg aagtggagaa ggacgccaca cagatcccta ttcccaaagt
    24721 cgaaggcgac tcggaatatg atatcgtctc ggaatatttt tttattatgg gcgaccataa
    24781 aaaatatcct tgcagtgtga tatctaattt ccccgataaa gatatggcca ttctcaaggt
    24841 cctgactgac cactggctcc ccctcccgat tgcaaatccc gacgaagcta gggtcggcga
    24901 acaagtctac gcgatcggtt ctccgctcgc cgtcctcttc aattctttct ctgacggtgt
    24961 catcagcggg aaagaccggg acatttccgc actcaccagc cctcttagat attccgcttc
    25021 cggtttcaac acgtggatcc agattaccgc ccacgttaac cccggtaatt ccggcggacc
    25081 gctcgttaat cggagaggcg aagtcatcgg aattaatacc cttagctaca attatctgac
    25141 gagcattagc ggcatatata tggccaaccg catcgacctt ctcaaggtcc ctgaattttg
    25201 gcttaagaag gcccccgaag aaaagaaata gagtaaaaac ttcatccttg tgccccacaa
    25261 cacgcttgtg gggctttttt atttacagcc ttgttaaaga aacagggcat gctactataa
    25321 ataagggcgc gttgtccttt tacaaaataa tactaaagaa ggaggaattt ttgtgaaaag
    25381 aaaagatgtt attttgtttt tgattatttg gcttattgct acttgtctcc ttacggctgt
    25441 cgttattcgc gattcttccg tgacctcgcc ttacaacaag catgagctta ctcgcagaga
    25501 gatctatctg gaatgcaaaa agtcggtggg gcttctcgac gttactcatc gcgcttattt
    25561 tggcgacccg agggcgcaaa tattttttgc tcttttcccg gtcgatttag actatggtgg
    25621 aagcggaacc gcttggttct ttgacaaaga ccgaccctat atcctcacga acgcgcacgt
    25681 tgtaaattgc ccgaacccga tagcattatt tttgtccgtc tggagtaagt tgcccgagac
    25741 acataaaaat tttctgaaaa aactcgggac catcaagatg gccgtagaaa gcacttatgt
    25801 cttcattgac cacgacggca agaaatattc atgcaagatc atcgccaacc tcgacaagct
    25861 gaacaatgtc gatttggccg ttctctccgt ggatattaag aaatggaagc cgctccccat
    25921 aggaaatccg gacagtatta aggtctgtga tgtcgtctat gtgatggggt cgcctctggg
    25981 ctatgttaat acttttaata tgggctacgt ctctgcaatc aatcgaaaga tcggctacaa
    26041 aagtaagaag gcacgttgga ctcttcatga cgcgcacatc aaccccggaa attccggcgg
    26101 accgatgctg aataaatact gcgaggttgt cggagttaat acactggtcc cgaccggcac
    26161 cagcggcatg tctcacgcct tgaacatcga cattcttcct cctctctatt tcctggttcg
    26221 ggacagaacc gacgagaaat atttttgcag gcctcccgaa aacaagaaat agaaccattc
    26281 acaccctcca cgagattttc gtggagtttt tttaccccgt aggaagtttt tagaaaaaac
    26341 aaaaaactac cttatgagta gccgtaggcg cttccgagaa atatcgtcca aaattcatat
    26401 aaaaacctat ttgaactttc ctactggggt catattattt tgcgaatttt tttgtgataa
    26461 gacacagcaa atctgtgcgc ttcactattg gcgataagaa tttctctttc atatttaccg
    26521 taaatttcgc gcttgcccag tattttcttt ggacgatgat attcatcttt tgtaacagca
    26581 accacgggta tattgagtct caggccctct agtatccttt ctgcggcatt cttttgggca
    26641 accgccccat ctaccactat aagatccgga agcctccact cttcgtggaa aaagcgccta
    26701 tccagtatct gtttaagatc gtttgtgtct ccagccaatt ttgtagggtc ttttatttta
    26761 aattttctat aatcagagcg tttaatttct ccattttcca agacggtcat tactccaatc
    26821 ctgtttatac cacccatatg agaaacatcg taagcctcta ttctgaaaac atctctattg
    26881 gttgattcgt ctccgagccc ctccttgttt tttattaaag caacgtcttc tatgtgctta
    26941 aggccaaata tttgcttttt aatctcgttc gctttttcaa attcgtggac attggcgtat
    27001 gagtgcatca ttctttcgag ggaattaaga atttttgtct tcttgccctc aaaaaataat
    27061 tttatgttct taatatttct cgcataatct gctttagtca cccggccctc gcaaacaccc
    27121 ggacacaaac ctatttgtgc ttcaaaacaa gccttaccta gtccaggcag gcctgcctgc
    27181 gcaggcaggc atttctctct aaatggaaat atttttctta ttatttttat tgcctctttc
    27241 aattctcctc cgtgcggaaa cgggccgaat atttcagaaa aattttttct atcttcggcg
    27301 aacagatctc tgcccctcat ggtcttaata cgcggatagt cttcagaggt gaccacgaca
    27361 taaaaataac tcttatcatc cttctcgcga atgttgtatt taggctgatg cttttttatg
    27421 agcgtagatt ctaaaataag cgcctcgagc acagaatcag tttttttaac cgaaatgccg
    27481 tcagcttgct ctaccatgtt tacaatagcc tgtcccctgg tttctataac cccacgcgaa
    27541 aaatagctcc taacgcggtc gcgcaaagag gtcgcttttc ctatatatat gatgcttctc
    27601 ccctttttaa acatataaac accgggagaa tcaggcagtt tgatagctaa tttttccatt
    27661 tgactttatt tatccattat gctataattg aaagagaaag taaaccctcc aaaattttat
    27721 accataaaga tatatagaca ctgaaccatc tggaaaggta aataaaacga cccaaatata
    27781 tctctagtac taaaaaattt aggcgggtaa aggttcctaa acaatcagta agttaagaaa
    27841 ggggtaccaa aaatgcagaa attcacgatt accgtcctcc tcgtctcgat cctcaccttc
    27901 tgcctcgcca cgccggtcta cgccggagga ttcttcaaca ggaaaacgtt ccagaccatc
    27961 ggcctcgccg tcgtcgtcga gcgcatcgcg gaatccgaac agaaccgcaa tcgtcgccac
    28021 gacgtggcga accgcaactc gaacgacctg aagcggaacc gcgccgagat cgagatgaag
    28081 aaggaacgcg accagtatgc aagggacaag cagatggcgg accttcgctt caagcaggag
    28141 ctcgagcggg aacgcgaact gcagaaggaa gaacgactcc accctgcgag ccgctacggc
    28201 gacgaggggt gcgaactcga cgacgaattc ggtgtcatca tcctgtgcga tcccaaaggt
    28261 cgctacaagg tggacgaata cgacgaaatc ttcacctccg cgaaagcagt caagcaatat
    28321 gtgaagagcc tcttccccga caggaaggtc gccatgttct ggcaaaaggt ggccgacctg
    28381 gagacccccc tcaacactta ctagattacg aagccccggg gcgatactga aaagtatcgc
    28441 ccctttttaa ttgccaaaac cccgcccgga gctttcccac aactaccccg tttatgtact
//
LOCUS       DAOTEX010000003        27301 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3118
            NODE_1147_length_27301_cov_4.075240, whole genome shotgun sequence.
ACCESSION   DAOTEX010000003 DAOTEX010000000
VERSION     DAOTEX010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868142
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 27301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 883
            CDSs (total)                      :: 833
            Genes (coding)                    :: 829
            CDSs (with protein)               :: 829
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27301
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1147_length_27301_cov_4.075240"
                     /isolate="AMR_MDS_3118"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..287
                     /locus_tag="P5056_00350"
     CDS             <1..287
                     /locus_tag="P5056_00350"
                     /inference="COORDINATES: protein motif:HMM:NF015377.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cysteine peptidase family C39 domain-containing
                     protein"
                     /protein_id="HRY62219.1"
                     /translation="EKDGATLEEIEKLLRDGVPAVVYFIEPSEDEHHYAVVTGLTDKE
                     IVLADPWNGADFRMMRDEFEKRWYGYEDEKNRWLLAVYNEPFCGCNCDKK"
     gene            351..1001
                     /locus_tag="P5056_00355"
     CDS             351..1001
                     /locus_tag="P5056_00355"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HRY62220.1"
                     /translation="MKTFDNKKLFIFTLAIISLGFVYGDFISGLDYDFDNLLSSIRTP
                     AGTTIFGYLTLLGDKYFLGVLSILLSLFIYKYWSKDTFLLKVFWAAFIMNTLSGLVIK
                     YMVGRDRPLGAEVLEGYTYSFPSGHATTAFFLYGFIAIILFNMRDIGKKKRYAATAFF
                     VSLFLLVGFSRLYLGVHFLSDVLGGYIIGWFWLNFLISFFNEISRDQASSVVKVVS"
     gene            1243..3558
                     /locus_tag="P5056_00360"
     CDS             1243..3558
                     /locus_tag="P5056_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP cone domain-containing protein"
                     /protein_id="HRY62221.1"
                     /translation="MPEEKKTTKKKPAKMPTKMVKRDGTIVDFDIKRITNAISKAMEA
                     AHEGKEEDARFVAHGVVKELAKMIADDKDYIPTVEDTQDLIENELIMMSFPKTAKAFI
                     IYREKRAELRRQTGVVVNEEVRTLVNESKKYFRNSLSEFVFYSSYSKWIPDKGRRETW
                     LETVGRYLEFMEENLGKKLSRDEYEEIREYMLSMKTLGSMRLLWAAGKAAKSTNVAAY
                     NCAFVAPKCWQDFGEIMYVLMCGTGIGFSVERQNVEMLPIIKRQNGKKVPKHFVEDSK
                     EGWANAFVLGLNTWAEGGDIEFDYSKVRPQGARLATMGGRASGPEPLKALLDFARGKM
                     ISRQGRHLTTLDVHDIVCKTGEVVVMGGVRRSALISLSELDDPEMRDAKSGQFFLAHP
                     ERSMANNSVVYNDKPTVVQFLEEWLSLMKSGSGERGIFNRGSLSKQMPERRWKLFEKD
                     SYDSGINPCGEIILKSKQFCNLSEIVARKEDTEKTLIEKARIAAILGTYQATLTKFPY
                     LSREWKKNCEDEALLGVSITGQWDCKAVRDPKTLEKMREMVIETNKKYAKRFGINQSA
                     AATCVKPSGNGSQLFDSSSGCHPRHSKYYIRRVRVENHNPLFMMLRDMGVPHAPEVGQ
                     DESNAKTYVLEFPIKSPEGAIFKNDLSAIDQLEYWKTLKTHYTEHNPSVTISVSNGEW
                     LKVGSAVYENWDIVGGLSFLPRDEHVYRLAPYETITEEEYKKRAAAFPEIDFSKLVLY
                     EYDDQTKGSKELACTAGLCEIEPLPTPGTKK"
     gene            complement(3609..3944)
                     /gene="rnpB"
                     /locus_tag="P5056_00365"
     ncRNA           complement(3609..3944)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="P5056_00365"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            4050..6503
                     /locus_tag="P5056_00370"
     CDS             4050..6503
                     /locus_tag="P5056_00370"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY62222.1"
                     /translation="MNKSIVDEKSAASDSQQEKIFVPRELSTMEKISFWILMACSFLL
                     PIFVYEPFSGFTPDFSKKAVLFCGAILSFLFWILARFEDGKISIPGGLFIKGSAALLA
                     AFVASSFAAGETGRSFYNSVFGSSFDTDTLSVFLAAFSLMFLSSIYFQSIKRLSYFYT
                     ALFFSFLIVFVAEAAHLYMGGTLIPGQGAVANLIGKWDDLGIFFGLCAAMSLAAIELM
                     HLNLNSKIFVWFVLLASLFSVAVVNYSAIWMILGVTSLLILVYAIFYNGNKTSVGSPM
                     SNIWSRPSFFIILICAALSFNMSGFSLLWNPQTKISETLSKYNIVNMDVRPSLETTLD
                     VAKNTYKDGWTKTLFGVGPNNFSGQWLKFKPTVVNDTIFWNVDFNMGIGRIPSYFITL
                     GLLGVLAWIFFLGSIFYLGFKTVLYSDIPKSSRFIVFVSLSSAIYLWLFSLVYVPDTV
                     IFALSFIVTGVLVSSLVRAHAIQNIEFSFLKDPRLGFVSVLILVVLVVSGVSGGYSVY
                     NKFLSNYFYQRSLSTLNSSGDFDKALADVKLALERDAQDTYYRSISEINLAKLNKFIS
                     EKRTMDKESVTLVQQIIVNASAAARDAIKLNDSNYLNWIALGRVGEAVIPLGSVIDGS
                     YELASDSYKKALELNPKNPTILLNLARLEIANKNVKQAKEYLGKALELKSNFTAALYL
                     LSQIEASEGNLAGAISKAERAYLFAPDDLGVLFQLGFLKFTSKDYEGAITALERAESI
                     NPQYSNAKYFLGLSYAKVGENSKALKEFRDISALNPENEEIKSIINNLLQGKDPFAAA
                     VSAKTGATKKGGLPVKEVE"
     gene            6514..8202
                     /locus_tag="P5056_00375"
     CDS             6514..8202
                     /locus_tag="P5056_00375"
                     /inference="COORDINATES: protein motif:HMM:NF015013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid II flippase MurJ"
                     /protein_id="HRY62223.1"
                     /translation="MVQKILKILNWEVGGLHEAAFLLGVSALSSQVLALLRDRLLAST
                     FGASRSLDIYYASFRVPDLIFISVGSFLAVTVIIPMIIQKIEDDKDNGFCNAREFLSQ
                     ILTVFSLTMVVTSAVAFTLMPKLSGLITPGFDAESTKSAVLLSRILLLSPFFLGLSNL
                     LGSVTQAFKKFFVYALSPLFYNLGIIIGIVFLYPIFGLPGLAMGVALGAFMHFAIQIP
                     VVVKTGMFPTFSLNIDFKEIKKVSTLSFPRTLALGINQLAIMFLVAEGSFMKAGSISI
                     FNFSYNLQSVPLAVIGVSYSVAALPEMSRIFSKKNISEFVEYIEGAFNHIIFWSLPIT
                     VLFIVLRAQIVRTILGAGMFSWNDTRLTIAALAIFALSVIAQNLIQLLDRVYYATGHT
                     IKPVMAKIVSSILIIVLAYFFVDVFNSNNLIRSTLEFVLRVGDVEGTEILMLPLAFSL
                     GTMFNLISLFIMFILDFDTFSKKFMTTILQVGGSSLVMGFVSYWLLGQISLLFEIDTL
                     WEIFLQGFVAGILGICVHLIILKLIKSSEFLEIERSLRKKFWKTETVIPNPEVI"
     gene            8290..10125
                     /gene="lepA"
                     /locus_tag="P5056_00380"
     CDS             8290..10125
                     /gene="lepA"
                     /locus_tag="P5056_00380"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HRY62224.1"
                     /translation="MDKNSSKNGISVGMGNIRNFSIIAHIDHGKSTLADRILEVTNTI
                     EKRKMMAQVLDSMELERERGITIKMQPVRIKYALGGVDYVLNLIDTPGHIDFSYEVSR
                     ALKAVEGAILLVDATQGVQAQTLSTLAVARESGLKIIPVINKVDLPSANVEETADEII
                     QILGCELEDIIKVSGKTGEGVDRLLEEIIKKVPAPKEIHEDSGSASALIFDFEYSNHQ
                     GIIVYVRVFDGAIKTGDDLVFSYSEERFNANEIGFFTPDKRISNEIRAGEIGYVVTGI
                     KNLKRATVGDTITKFTKRLPPLTGYMQPKPVVWASVYPESQDDFGLLKHSLEKLKLSD
                     SSLDFEEESSMSLGRGFKCGFLGMLHLEIITERLRREHNINIIIAAPTVGYEVQMKGS
                     DTRELIYSAAHFPDKDKMEKVFEPWVEARIILPPEYVGDIMQMLYDHEAIVGNTEIFG
                     DNRNDLIVEMPLRELMRNFFDELKSVSSGFASLSYKMIGMREADVARMDVLLADEPVT
                     AFSRIISRKKTDTEANDIVDRLKDILPRQMFDFKIQAKIDGRIVSSRHMSGMKKDVAG
                     YLYGGDITRKMKLREKQKKGKKKMKRLGSVNIPPDVFLKVMRGKE"
     gene            10508..10909
                     /locus_tag="P5056_00385"
     CDS             10508..10909
                     /locus_tag="P5056_00385"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HRY62225.1"
                     /translation="MRDFQGRKRWHKVLRSDIFLFAVLIVNIILIKSVWSVYNKDAAA
                     RLNREEAETTLANLQKKKAGLEKEIARLNTDRGVEEELRRRFQVVKPGEQVLVIVDKE
                     ESKAPVSSAKPEGATEHIWNKLVGLLYFWQK"
     gene            11151..11221
                     /locus_tag="P5056_00390"
     tRNA            11151..11221
                     /locus_tag="P5056_00390"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11183..11185,aa:Gly,seq:ccc)
     gene            11413..11697
                     /locus_tag="P5056_00395"
     CDS             11413..11697
                     /locus_tag="P5056_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNHCP domain-containing protein"
                     /protein_id="HRY62226.1"
                     /translation="MSQKFQRRKEDFACEKCGNFVVGTGYTNHCPKCLWGKHVDIFPG
                     DRANKCQGMMKPISIINEKGDFVLIHKCEKCGEEKRNRAAADDDLSCILH"
     gene            11744..11815
                     /locus_tag="P5056_00400"
     tRNA            11744..11815
                     /locus_tag="P5056_00400"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11777..11779,aa:Arg,seq:cct)
     gene            11883..12179
                     /locus_tag="P5056_00405"
     CDS             11883..12179
                     /locus_tag="P5056_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62227.1"
                     /translation="MEEKLDELLKLTKENNKILRGMRSSQRWASFFNYIYYALIIGSF
                     IGAYYYLQPIVDKAMQSLQGVASGIQDVQGAAAGARNINISPDTIDQLQKTLNL"
     gene            12233..12308
                     /locus_tag="P5056_00410"
     tRNA            12233..12308
                     /locus_tag="P5056_00410"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12266..12268,aa:Phe,seq:gaa)
     gene            complement(12552..13007)
                     /locus_tag="P5056_00415"
     CDS             complement(12552..13007)
                     /locus_tag="P5056_00415"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HRY62228.1"
                     /translation="MKNSKSSFFERLTGSTDARNKIEIDDSLDLQEEVSAITKQEDDA
                     ELAIDMYQTPTEIIVRTMVAGVKPEDLQVSIGRDIITIKGARSSHHEVSEKDFFAKEL
                     YWGSFTRTIMLPSEVDADNAEAVEKHGLLTIKLPKIEKDKTKSLKIKSM"
     gene            complement(13015..13701)
                     /locus_tag="P5056_00420"
     CDS             complement(13015..13701)
                     /locus_tag="P5056_00420"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HRY62229.1"
                     /translation="MAGSITYDNVFWSLLAGILPALFWLWFWLREDRKNPEPRSLLLK
                     TFLAGTLSVFPAFALQQFFAKGVNSEYLLIFIIWPLTEELVKYFTAYFVALRTKDFDE
                     PIDAIIYLITAALGFAAAENFFFLVKPTFSIMSLITANMRFIGATLLHLLTSATVGSF
                     IALNFYKDKAHKKISLILGLLTATVLHSLFNAFIIKDNGGNIFAVFSTLWLFTIALLL
                     LCEKIKSLKK"
     gene            complement(13686..15905)
                     /locus_tag="P5056_00425"
     CDS             complement(13686..15905)
                     /locus_tag="P5056_00425"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HRY62230.1"
                     /translation="MENKQPNIDERLLKPYESKNTESKIYKIWEDSGLFNPDECIKKG
                     VTKADAPVFSMVLPPPNVTGTLHMGHALMLTVEDILVRFNRMRGKRTLWVPGTDHAAI
                     ATQSKVEKIIYKEEGKNRHDLGREEFLKRVEKFAQDSHDTIVNQMKTMGVSVDWSREA
                     YTLDEKRNLAVRTAFKKMYDEGLIYRGHRIVNWDPKGQTTVSDDEVVYEERKAKFYTF
                     KYSKDFPISISTTRPETKVGDTAVAVHPDDPRYKKFVGKEYDANFAGVHIHVKIVADE
                     TVDKEFGTGALGVTPAHSMTDWDIAERHDLPRPQVINEYAKMMVDAPLLKGKKTTEAR
                     EIVVEWLRQEGLLEKEEEVSQNISTAERTGGIIEPLPKLQWFLNVNKEFTLADSKIDG
                     IPSGSDTTLKEIMKKTVENGQINIVPDHFKKTYFHWIENLRDWCISRQIWFGHRIPVW
                     YKGEEIYCGINAPEGEGWTQDSDTLDTWFSSGLWTFSVLGWPEKTEDLRIYHPTDILE
                     TGYEILFFWVARMILMTGYILGDIPFKKVYLHGTVRDAQGRKMSKSLGNGIDPIVIAD
                     KYGADAARIALVIGAGPGTDTRIYEDKIKGQKHFANKIWNVARFVLSNVNSENLGEKP
                     EKFSPENEAILAELKNVAKQATANIENDRLHIAAEEIYQYFWHVFADKIIEDSKEKLN
                     GGKANETRWMLKTVLETSLKLIHPFMPFVTEEVWEIVNNHFGNKTKNLIMVGEWPEA"
     gene            complement(15931..17097)
                     /gene="tsaD"
                     /locus_tag="P5056_00430"
     CDS             complement(15931..17097)
                     /gene="tsaD"
                     /locus_tag="P5056_00430"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HRY62231.1"
                     /translation="MKILAIETSCDDTAIAIIEANGGLKKPSFKVLSSQTHSQIELHA
                     KWQGVVPFLAKREHSINLVPLLEASLEKAGLLKTAAKEKTLPGELHKILEKEPELLKN
                     MELLLPEIEIPKIDAIAVTYGPGLEPALWVGVNMARALSLCWKKPIVPVNHMEGHALS
                     SLLAEKKTRGIKFPSIALLVSGGHTELVLMKDWMKYKILGSTRDDAAGEAFDKVARIL
                     GLTYPGGPKISKLAETFDIKNSTGIKFPRPMINSDDFDFSFSGLKTSVLYKAKELAEL
                     TDELKKEFAFEFEQAVIDTLVSKTKKAINKFKAKSLVIGGGVSANKKLRVSLTEMIKK
                     DLADTELLIPESSLSVDNAVMIGIAGYFRAQKKKPVFSLPLIKRIKAEGNLLLK"
     gene            17233..17652
                     /locus_tag="P5056_00435"
     CDS             17233..17652
                     /locus_tag="P5056_00435"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRY62232.1"
                     /translation="MFIRPLKIQDESQVVALMENFLFLTKKEVLFNAAECLNDSNVHG
                     LVIEDNGNVVGHGALVVSRIPSKGLYGIVHDVVVSDQYRGQGLGRKLVNELVDLARSL
                     KLPLIVLTSNKARVAARNLYESIGFVEKDTGFFEMVL"
     gene            complement(17927..18000)
                     /locus_tag="P5056_00440"
     tRNA            complement(17927..18000)
                     /locus_tag="P5056_00440"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17964..17966),aa:Val,seq:gac)
     gene            complement(18027..18578)
                     /locus_tag="P5056_00445"
     CDS             complement(18027..18578)
                     /locus_tag="P5056_00445"
                     /inference="COORDINATES: protein motif:HMM:NF013468.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sua5/YciO/YrdC/YwlC family protein"
                     /protein_id="HRY62233.1"
                     /translation="MKIIDKKYIKNNEEKIIREIKAGKIFIYPTDTIYGIGANAKKST
                     SVAKIRTIKERELKPFSVIAPNKKWIKDNFVFNKKIEEWTKKLPGPLTLFLDRKNTAC
                     VSEEVNPADDSLGVRIPKHWFTKIIQKAGVPFITTSVNKSGQSYMRRMEDVDSGTLNS
                     VDYIVYEGEKANKESKKVDLRKI"
     gene            18681..19052
                     /locus_tag="P5056_00450"
     CDS             18681..19052
                     /locus_tag="P5056_00450"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY62234.1"
                     /translation="MKVLIVEDDPLLVEMVKAKLKKDGYEVFSAFNGEEGLCRVKECF
                     PDVIILDINMPIMNGFELLKILKKDNVFQKIPVILFSSTGDKEDNLEMGKRLGASGFL
                     VKSQLSLNDIPAKIKEVIGQK"
     gene            complement(19244..19555)
                     /locus_tag="P5056_00455"
     CDS             complement(19244..19555)
                     /locus_tag="P5056_00455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62235.1"
                     /translation="MDKTITANKEHRKIILLKETYEEITKILKIIDLFNRTFSRKDTR
                     PEEIRAKVLMTIKNLEEKTDQYYNLSKEIESEVTLELLDMNQVVIHAISVIHQKRKEY
                     S"
     gene            complement(20060..20653)
                     /locus_tag="P5056_00460"
     CDS             complement(20060..20653)
                     /locus_tag="P5056_00460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62236.1"
                     /translation="MNKREKRIKKEQKDIQELLKIASLNELTDTGSDNIYTFAHLGGQ
                     RQKLIRMMVLETYLQIDTKMNILIANFFFHHCQDDCEEVQKITTSKEFIVFSEKILNE
                     MPFLRKVSSIKSFIFVPKKIVTDLNKLNDLRNAMAHALIPENLGTERVTYKGKSIFTL
                     EGVRSFNKDMKSILSFLQRYFIQAFKDNKKKLGSNHS"
     gene            complement(20749..21228)
                     /locus_tag="P5056_00465"
     CDS             complement(20749..21228)
                     /locus_tag="P5056_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62237.1"
                     /translation="MTKKKAKKDRIIITEDFVQDRVIRHLEKNGWSKSLKSKELWEQG
                     VDIKVCNNKFSRYWLVEAKGDPSIKVLSPSGSQSSSFNSALGQIVTRMHRNGARGYKY
                     GYKYGIALPSSFKRMAIRKIPFDVMDKLNLYLFFVDKNGNVEELDWKKVKEIDGSKR"
     gene            complement(21243..23927)
                     /locus_tag="P5056_00470"
     CDS             complement(21243..23927)
                     /locus_tag="P5056_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018203552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HRY62238.1"
                     /translation="MALHKDFPRDPYAILNPEIRWFPADEDLREKGFDKLIPPLVAEL
                     RKQVKDWRDHNYKDASETSRALLTWWFQEEHTLYNKDGSSYNFRYYFSQREALETVVW
                     LYEVAGVKDKYDLIRYNSTGVLSPQMFTEDWLRFVIKMATGAGKTKVMSLVIAWAYFH
                     KLYEKGSELAKNFLLIAPNVIVFERIKNDFEGNKIFFLDPVLPDNGYMGQNWQDDFQV
                     TLHLQDELKNVSDTGNIFLTNIHRVFESEVQDYSAEDEDTSAYFLGNKPVTKTNDAMV
                     DLGMVVRDINELIVINDEAHHIHEENAWLKSIRDIDNKLKQKGGGLALQIDVTATPKK
                     KDGSIFVQVISDYPLVEAIHQRVVKNPVVPDEASRGKLKPNQSTLFSEKYRDYINLGV
                     EEWRKTYKQLEPTGKKSILFIMTDDTKNCDEVADYLQTSFPDLKGSVLTIHTNKSGEI
                     SETVTGRNKEELEQLRKQSNEIDSWESPYKVIVSVMMLKEGWDVKNVTTIVGLRPYAS
                     DSKILPEQTLGRGLRRMYFGRDDIDEYVSVIGTPAFMDFVESIKGEGVLLEKRLMGSG
                     SKPISPMVIEIDKENAKKDIGKLDIEIPVMTARIQREYKNLAELDVSSFGNKKVKVKI
                     FSEQEKREIVFKDVVAEEVHHTTILSGEIEPNYQSVVGFFAQAIMREMRLFGCYDILF
                     GKVKDFVENYMFESKVELTDLNVLRNLSEVEYIKLIKDGFKKSINELTVQDKGDTEIK
                     NYIKISDARPFVVNDKSFLIPQKSVFNRIVGDSNFELEFSAFLEKCDDIISFAKNFQN
                     KEASALRIEYKNAEGFIAIYYPDFFVKKDEKEVYIIETKGREEEDDKIKFERLQKWCE
                     DVNSRQSRVVYKPLYVKQEDYEKNPSKNFDELTRLFTK"
     gene            complement(24052..24810)
                     /locus_tag="P5056_00475"
     CDS             complement(24052..24810)
                     /locus_tag="P5056_00475"
                     /inference="COORDINATES: protein motif:HMM:NF014045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HRY62239.1"
                     /translation="MFKPYTPKIEKIAKLFPKVIEELDSIFNENTKIYIDWQNVLHWQ
                     EKLGWHFSLKRLKQFFNSFDTIQSVIIYTGTLETDERSLEQIKEIENCKYILSTKPVK
                     MMKISIDVTSIPDYSPAILKNFMKKRFLSKLDIDTVKYLNKKMSLLNKQGITYIEEPK
                     CNFDVEMGVDMLLDCERNNTKSFVLWSGDSDFASPINQIKNSGKRAILFAVSGKVAPE
                     LDETGVFIFDVKKIKEFVCFPKEIPLAVKDKLAL"
     gene            complement(24815..25027)
                     /locus_tag="P5056_00480"
     CDS             complement(24815..25027)
                     /locus_tag="P5056_00480"
                     /inference="COORDINATES: protein motif:HMM:NF025005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KTSC domain-containing protein"
                     /protein_id="HRY62240.1"
                     /translation="MNRISVVSSNINSIGYDEVTSTLEVEFTSGDIYHYLNIPKYLFQ
                     QFLHSSSHGQFLNDFIRYNYKYQKIN"
     gene            complement(25033..27231)
                     /locus_tag="P5056_00485"
     CDS             complement(25033..27231)
                     /locus_tag="P5056_00485"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020257978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HRY62241.1"
                     /translation="MTNSKNNQIFLSDNEKRDITKFLEANKPLPDKYRFLLFGDKNEV
                     ELVWNGKSNEVTNVVLPFQVIEQIDEPREGKEFGDGRGTLFDFSGKQVAGWTNKLIWG
                     DNKLILSSLKNGPMRKEIEAQGGLKLIYIDPPFDVGADFSMDITIGDKEDGESFTKKP
                     SVIEEVAFRDTWGNGVDSYVAMIYERLKLMHELLAENGSIYVHCGIQVNHYVRLAMEE
                     IFGKNNLVSEIIWSYGTPSGGRASGTKLVKIHEYILHFAKNYDNRIENKIYLPYAEKY
                     LKDWFKYTDENGRVYRQRIRGKSSDGKPIYERQYLDESPGEPASTVWKDIKQIYADPR
                     AFKANQGDYSEILDYPTQKPESLIERIINASSNEGDLVADFFCGSGTTLAVAEKLGRK
                     WIGSDLGKFGIHTSRKRMIGIQRELKKAGKDFRAFEILNVGRYERESFLDTNNDLRAE
                     EKAKQAERKEKDFIKLILSAYKAEAVDSFVNVVGKKRDRLVAIGPINLPVSQKFIQEI
                     VKELKEKNITKVDVLGFDYEMGLIFDEFVTQGMDIAFKIIPREVFDKRAVERGQVNFY
                     DVAYIEAKPIVTGRGNMKELSIELADFSVFYNQDNGAVEEELKAGRSKVIIENGQVIK
                     VTKDKDTELVSREVLTKKWSDWIDYWAVDYNFADRKEIIKVIENDKEKEVWTGDYIFD
                     NEWQSFRTKKNRNLEFKSAPKELPKGDYKVAVKVVDIFGNDTTRVIDVKI"
BASE COUNT         7223 a         5604 c         5885 g         8589 t
ORIGIN      
        1 gtgagaaaga cggcgccaca ctggaagaga tcgaaaaatt attgagagac ggcgttcctg
       61 cggtagtgta tttcatagag ccatccgagg atgagcacca ttatgcagtc gtgaccggcc
      121 tcacggacaa agaaattgtt ctcgcggacc cttggaacgg agcggatttt cgaatgatga
      181 gagacgagtt tgagaaaaga tggtatggat acgaggacga gaagaacagg tggctcctcg
      241 ccgtatataa tgaaccattt tgcggatgca attgtgacaa aaaataaaaa taggcgcgca
      301 atgaggcgcg tctttttttt ggatcaaaaa tcagctataa tcgtaatatt atgaaaacat
      361 ttgataataa aaagctgttt atcttcaccc ttgcgattat ttcgctcggt tttgtgtatg
      421 gggacttcat ttccggacta gactacgatt ttgacaatct ccttagcagt attaggacgc
      481 ccgccggcac gaccattttc ggctacttga cgcttttggg agacaaatac ttcctagggg
      541 ttttgagcat cctgctttct ctctttattt ataaatactg gtctaaggac acgtttttgc
      601 taaaggtatt ttgggccgcg tttataatga acacgctttc cggcctcgtc ataaagtata
      661 tggtgggcag ggacaggccg ctcggagcgg aggttctgga gggttataca tattcattcc
      721 caagcgggca cgctacgaca gcatttttct tgtatggatt tattgcgata attctcttta
      781 atatgagaga tatcggaaag aagaagcgat atgccgcgac tgcgtttttt gtgtcgctct
      841 ttcttctcgt aggtttcagc cgtttgtatc tcggtgtgca tttcttgagc gacgttttgg
      901 gcggatatat tatcggatgg ttctggctca atttcctgat atcttttttc aatgaaattt
      961 ctagagacca ggcgtcaagt gtggtcaagg tggtgtccta gattccttgc tccgatttta
     1021 gccttaaaaa tacttgatat ttcggcgggg tttttgattt aaaaataaat ctgtttcaga
     1081 acgatttttt tagcgcttta tccacagcgc tttttatttt tttaaattga tttgaactaa
     1141 aatgaaaaca ttattagaga tattattttt ccgacaaaat tttttatata aaaaagttca
     1201 caaaaattat taaaaatttt ttgaaggata aatcaaaaca acatgccaga agaaaaaaag
     1261 acgacgaaga agaagccggc gaaaatgccg acaaagatgg ttaagaggga cggaactatt
     1321 gttgattttg atataaaaag aattacgaac gccatatcca aggcgatgga agccgcgcat
     1381 gaaggcaagg aagaggatgc cagatttgtg gcgcacggag tcgtgaaaga attggccaaa
     1441 atgatagccg atgacaagga ctatatcccg accgttgaag acacgcagga tctcatagag
     1501 aacgagctta tcatgatgag cttcccgaag accgccaagg cattcattat atatagagag
     1561 aagagggccg agcttaggcg ccagacgggt gtcgtggtga atgaggaggt ccgcacgctt
     1621 gtgaatgaaa gcaagaaata cttcagaaac tcgctatcag agttcgtgtt ttacagctct
     1681 tactcaaagt ggataccgga taaggggaga cgagagacat ggctcgagac tgttggaaga
     1741 tatttggaat ttatggagga gaatctcggc aagaaacttt cgcgagatga atacgaggag
     1801 atcagagaat atatgctctc gatgaagacg ctcggctcga tgagactttt gtgggccgcg
     1861 ggcaaggccg ccaaatctac gaacgtcgcc gcttacaact gcgcattcgt cgctccgaag
     1921 tgctggcagg atttcggcga gataatgtat gtcctcatgt gcgggacggg aatcggattt
     1981 tcggttgaga ggcagaacgt ggagatgttg ccgataatca agagacagaa cggcaagaag
     2041 gtgccaaaac attttgtaga agacagcaag gagggctggg ccaacgcttt tgtgctcggg
     2101 cttaatactt gggcggaagg aggagacatt gaattcgatt attctaaagt aaggcctcag
     2161 ggtgcgagac tcgctacgat gggaggcagg gcatccggtc cggagccgct caaggcgcta
     2221 ctcgattttg ccagaggaaa gatgatctca aggcagggca gacacttaac gacactcgac
     2281 gtgcacgata ttgtctgcaa gacaggcgag gtggtggtga tgggcggagt aaggagaagc
     2341 gcgcttatat ctctttcaga attggacgat ccggaaatgc gagacgcgaa gagcgggcag
     2401 ttcttcttgg ctcatcctga gagaagcatg gcgaacaact ctgttgtgta taacgacaag
     2461 ccgaccgtcg tgcagttctt ggaagagtgg ctcagcttga tgaaatccgg ttcgggagaa
     2521 agaggaattt tcaacagggg aagcctatca aagcagatgc cggagagaag gtggaaattg
     2581 tttgaaaaag attcttatga tagcggtatc aatccgtgcg gagaaattat tttgaaatca
     2641 aagcagttct gcaacttgag cgaaatagtc gccagaaaag aagacacaga aaagacattg
     2701 atagagaagg cgaggattgc ggccattttg gggacatatc aggccaccct cacgaagttc
     2761 ccatatcttt ccagggagtg gaagaaaaat tgcgaagacg aggcgctcct cggagtatct
     2821 atcacgggcc agtgggattg taaggcggtc cgagatccga agacccttga gaagatgagg
     2881 gagatggtaa tagagacgaa caagaaatac gcgaagaggt ttggaataaa tcagtccgcg
     2941 gccgctacgt gcgtgaagcc gtccggcaac ggttcacagc tctttgattc ttcttcggga
     3001 tgtcatccaa ggcactcaaa atattatata aggagggtta gagtagagaa ccacaacccg
     3061 ctgtttatga tgctccggga tatgggcgtg cctcatgctc cggaagtcgg acaggacgaa
     3121 agcaacgcga agacatatgt attggaattc cctatcaagt ctcctgaggg cgcgatattc
     3181 aagaacgacc tctctgcgat agaccagctc gaatattgga agacattgaa gacgcattac
     3241 acggagcaca acccttcggt aacgatatcg gtcagcaacg gggagtggct caaggtgggt
     3301 agcgctgttt acgagaactg ggacattgtc ggagggcttt ctttcttgcc tcgagatgag
     3361 cacgtttata gattggctcc atacgagacg attacggaag aggaatacaa gaagcgcgcc
     3421 gcggctttcc ctgagataga tttctccaag ctggtgctct atgaatacga cgaccagacg
     3481 aaaggttcaa aagagcttgc ttgcacagcc ggtctttgcg agatagagcc gttgcctact
     3541 ccggggacaa agaaataaga ataaattttt gtagaaatga aaagaccgca ctttcgtgcg
     3601 gatttttcat gttctgtgcc cgtaagccga attctgtttc gataattatt tatctggtcc
     3661 ttgcattact gcagggatct agcggcactc cgatatccga agataccagt acggccttgc
     3721 actcaggtaa ggattttgcc gtttcaccct ctttatttct aaagagatac accctagaaa
     3781 atgacttgcg ccaccttttg gggtggatat cgattttcat caatatccgt cactgttcgc
     3841 acctcggcga ttactcgcga tggggattac ccactaccat ttaatgagcg ttcggacttt
     3901 cctcccggcc cttttcgggg atgagcaatt atccggcaca ggcaaatata tattaacaga
     3961 caaaaatgga attgtctagt ttgtatcggt ggtgttaaat attaaaatta gacttatatt
     4021 agcgaatcta ttaataattt tataaaaaaa tgaacaagtc tatcgttgac gaaaagtcgg
     4081 ccgcttctga ttctcagcaa gaaaaaattt ttgttccgag ggagttatca acgatggaaa
     4141 aaatatcttt ttggatatta atggcatgtt cttttttgtt gccgatattt gtctacgagc
     4201 cgttctccgg cttcactcct gatttcagca agaaggccgt tctgttttgc ggtgcgatac
     4261 tctcgttttt gttttggatt ttagcaagat ttgaagacgg caaaatatct attccggggg
     4321 gcttgtttat aaagggctcc gcggctctac tcgcggcgtt cgttgcgtct tctttcgccg
     4381 caggagagac gggcaggagt ttctacaact ctgtttttgg atctagtttt gacaccgaca
     4441 ctttgtcagt atttttggcg gcgttttctt tgatgtttct atcgtctata tatttccaga
     4501 gtataaagag gctttcttat ttttatacgg cgttattttt ctcattcctt atcgtctttg
     4561 tggctgaagc ggctcactta tatatgggag gcacgttgat accgggccaa ggcgccgtcg
     4621 cgaaccttat cggcaagtgg gacgacctcg gaatcttctt cggtctttgt gcggcgatgt
     4681 ctctcgcggc gatagagttg atgcatctta atcttaactc aaagatattt gtttggtttg
     4741 tgcttcttgc ttctctcttt tctgtcgcgg tagtcaatta ttccgcgata tggatgattc
     4801 tcggtgtaac ctcgcttctc attcttgtat atgcgatttt ttacaacggc aacaagactt
     4861 ccgtcgggtc gcctatgtca aatatttggt ctcggccttc attctttatc attttgattt
     4921 gcgccgctct ttcttttaat atgtccggtt tttctctttt gtggaatccc cagacgaaga
     4981 taagcgagac tttgtctaag tataatattg taaatatgga tgtaaggccc tctctcgaga
     5041 caacgcttga tgtggccaag aacacataca aagacgggtg gacaaagact ctttttggcg
     5101 taggtccgaa taatttttcc gggcagtggt tgaaatttaa gccgacggtg gtgaatgaca
     5161 caatcttttg gaacgtagat ttcaatatgg gtatcggccg cataccatct tacttcataa
     5221 cgcttggcct tttgggtgtc ttggcttgga tatttttcct cgggtctata ttttatctcg
     5281 gatttaagac ggtgctatat tcggatatac cgaagtcttc cagatttatc gtttttgttt
     5341 ctctgtcatc cgcaatatat ctatggctat tctctctggt gtatgttccg gacacggtca
     5401 tcttcgccct ttcatttatc gtcacgggcg tgctcgtctc atctcttgtg cgagcacacg
     5461 cgatacagaa tatcgaattt tctttcttga aagatccgcg gctaggattt gtttcggtgc
     5521 tcattttggt ggtgcttgta gtttccggtg tgtccggcgg gtattctgtc tataataaat
     5581 tcttgtctaa ttatttttat caaagaagtt tgtccaccct caattcttcc ggagattttg
     5641 ataaggctct cgcggatgtg aaattggcgc tagaacgcga cgcgcaggat acatattata
     5701 gaagcatttc cgagatcaac ttggccaaat tgaataaatt tatttcagaa aagagaacga
     5761 tggataagga atccgtgaca ctcgttcaac agataattgt gaatgcctcc gcggcggcaa
     5821 gagatgcgat aaaactgaac gattcaaatt acctgaactg gattgctttg ggtagagtag
     5881 gggaagctgt aattcccctc ggatccgtga ttgacggctc ttatgaattg gcttccgatt
     5941 cttacaaaaa agcgttggag ttaaatccta agaacccgac aatacttttg aatttggccc
     6001 gtttagagat tgccaacaag aatgtaaaac aagcgaaaga gtatctcggg aaggcgcttg
     6061 aattaaagag caactttacc gccgctctgt atctcttgtc tcagatagag gctagcgagg
     6121 gtaatcttgc cggagctata agcaaggctg agcgtgctta tctattcgcg ccggatgatc
     6181 tcggtgtcct gttccagctc ggattcctca agttcacgag taaagattac gagggggcca
     6241 taaccgcact tgaaagagcg gaatctataa atcctcaata ttcgaacgca aaatatttct
     6301 tgggattgag ctacgcaaaa gtcggcgaga acagcaaggc gctcaaagaa ttcagagaca
     6361 tcagtgcgct gaaccccgag aacgaagaaa taaaaagcat cataaataat cttttgcagg
     6421 gtaaagatcc gtttgccgcc gcagtgtccg caaaaaccgg cgccaccaag aaaggcggct
     6481 tgcctgttaa agaggtggaa taattcgcta tagatggttc aaaagatctt aaaaattctc
     6541 aattgggaag tcggcggttt gcatgaagcg gcttttttgc tcggggtgtc ggctctgtct
     6601 tcacaagtat tggcgcttct tcgagatagg ctattggcga gcacttttgg cgccagccgt
     6661 tctctcgata tttattatgc ttctttccgc gtgcccgacc tcatctttat ttccgtcggc
     6721 tccttcctcg ccgtgacggt aataataccg atgattatcc aaaaaataga ggacgacaaa
     6781 gacaacggct tttgcaacgc gcgagaattt ttgagccaaa tattgaccgt tttttccctg
     6841 acgatggtcg tgacgagcgc ggtcgccttc actcttatgc cgaagttatc cgggctcata
     6901 actcccggtt ttgatgccga atcgacaaag tccgcggtgt tgctttcccg aatcttgctt
     6961 ctctcgccgt ttttcctggg actttctaac ttattgggaa gcgtgacgca ggcatttaag
     7021 aaattttttg tttacgctct cagccctctt ttttacaacc tcggcattat tatcggtata
     7081 gtatttttgt atcctatatt cggtctcccg gggcttgcta tgggtgtcgc gctcggggct
     7141 tttatgcact ttgcaataca gatccctgtt gttgtgaaga cgggaatgtt cccaacgttt
     7201 tcgctcaaca ttgatttcaa agaaataaag aaagtttcta cgctgtcttt tccgaggaca
     7261 cttgcgctcg gcataaatca gctggctata atgttccttg ttgcggaggg gtcttttatg
     7321 aaggccggtt ccatttctat ttttaatttt tcttacaatt tgcagtcggt gccgcttgcg
     7381 gtcatcgggg ttagctattc ggtggcggct ttgcccgaga tgagcaggat cttttcaaag
     7441 aaaaatattt ccgagtttgt tgaatatata gagggggcgt tcaatcacat aattttctgg
     7501 tctttgccga taaccgttct cttcatcgtg ctccgcgcgc agatagtgag gaccatattg
     7561 ggcgcgggaa tgttcagctg gaacgacacg aggctcacga ttgcggcgct cgccatattc
     7621 gcgctgtctg ttatcgcgca aaatctcata cagctcctcg atagggttta ttatgcgacg
     7681 gggcacacga taaagccggt tatggcgaaa atcgtttcat ccattctcat aatcgtcttg
     7741 gcgtatttct tcgttgatgt gtttaattca aacaacctca ttaggtccac gctcgaattt
     7801 gttttgcgag taggggacgt ggagggcaca gagatactca tgcttccgct tgctttttct
     7861 ttgggaacaa tgttcaacct gatatctctt ttcattatgt tcattcttga tttcgataca
     7921 ttctcaaaga aatttatgac cactattttg caagtcggcg gttcatcgct cgtgatgggg
     7981 ttcgtttcgt attggcttct cggtcagata tcacttcttt ttgaaataga cactttgtgg
     8041 gaaatattct tgcagggttt tgtcgccggt atcttgggta tatgcgtgca cctcatcatt
     8101 ttgaaactta ttaaaagctc ggagttcctg gagatagaga ggtccttgcg caagaagttc
     8161 tggaaaactg aaacggtcat tccaaaccct gaagttatat aaaaaaacac gcggggcgcg
     8221 gcgccaaaca aaaaacgaaa acagcccttg ttcttcgggg ctttaacata tatattagat
     8281 ggacaataca tggataaaaa ttcgtcaaaa aacggaatat cggtggggat gggaaatatc
     8341 aggaatttct ctattattgc ccatatagac cacggtaaaa gcacgcttgc cgacaggatc
     8401 ctcgaggtga ctaatacgat agaaaagagg aaaatgatgg ctcaggtgct agatagcatg
     8461 gagcttgaaa gagagcgtgg tattaccatt aaaatgcagc ctgtgcgcat aaaatacgct
     8521 ctaggcggcg ttgattatgt tttgaacctc atagacaccc ccgggcatat agacttctcc
     8581 tacgaggttt ctagggcctt gaaggctgta gagggggcaa ttcttcttgt agatgctact
     8641 cagggagtcc aggcgcagac cttgtccact ttggctgtcg ccagagagtc cggcctaaag
     8701 ataatcccgg tcatcaacaa agttgatttg ccttctgcga acgtcgaaga aacggctgat
     8761 gaaataattc aaatattggg ttgcgagcta gaagatatta ttaaagtttc cggaaagacg
     8821 ggagagggtg tcgacaggct tcttgaagag ataattaaaa aagttccggc gccaaaagag
     8881 atacacgagg atagcggaag tgccagcgct ctcatcttcg acttcgaata ttcaaatcat
     8941 cagggaataa ttgtttacgt cagagttttt gacggcgcca taaagacagg agacgatctg
     9001 gttttttcat acagcgaaga aagatttaat gctaacgaaa tcggattttt cactcccgac
     9061 aaaagaattt caaacgagat aagagcgggc gagattggct atgtagtcac gggtataaaa
     9121 aatctgaaga gggcgaccgt cggagatacg ataaccaagt tcacaaaaag acttccgcct
     9181 cttaccggat atatgcaacc aaagcctgtc gtatgggctt ctgtatatcc cgagagtcag
     9241 gacgacttcg gtctcttgaa acactcgctg gaaaagttga agctttctga ttcctcgctc
     9301 gacttcgaag aggagtcatc aatgtctttg ggcagaggct ttaagtgtgg attcctcgga
     9361 atgttacacc tcgaaataat caccgagagg ctgagaagag aacacaatat aaatattatt
     9421 attgccgctc ctaccgtcgg ttatgaagtg cagatgaagg ggtccgatac tagagagctt
     9481 atttattccg cggcgcattt tccggataag gataaaatgg agaaagtctt tgagccttgg
     9541 gtggaggcga gaataatttt gccgcctgaa tatgtcggag atattatgca gatgctttat
     9601 gaccacgagg cgatagtcgg gaacactgaa atattcggag acaacagaaa cgacctcatc
     9661 gtagagatgc cgcttcgaga acttatgagg aatttctttg atgagctaaa gagcgtgtct
     9721 tccggattcg catcgctttc atacaaaatg atcgggatga gggaggccga cgtggccaga
     9781 atggatgttt tgcttgcgga cgagccggtc acggcgtttt caagaattat ctcccgcaaa
     9841 aagacagaca cggaggcgaa cgatattgtc gataggctga aagatatttt gccgagacag
     9901 atgttcgatt ttaaaatcca ggccaagata gacggcagga tcgtatcttc gaggcacatg
     9961 tcgggaatga aaaaagatgt cgccggatat ttgtatggag gagatattac gagaaagatg
    10021 aagctcagag agaagcaaaa gaaaggaaaa aagaaaatga aaagactcgg gtctgtgaat
    10081 attccacccg atgtgtttct aaaggttatg agaggcaagg agtaaaatcc tcaaatttgg
    10141 cgcataactt cgtcgaacgc gaggatttta tcttttagct aaataagccg gggaggtaca
    10201 tacctaccat gcttattatg acagctaagc aaataatagc ccaaatgatg ttaactgcct
    10261 tcttgttctt tttcatcaac ataattaaga aatcacaatt aataaatgac cgctagatat
    10321 aaaataaatt gataaatgat tgcgcttggc tactgcgcca tccaaaattc tactacggcc
    10381 tttcatacat attctcggcg tatctagata cgctttcgaa tttgtattca ggcctcgcta
    10441 cgaattttga atagctcgat acgccatcat ttcttaactg ttatttctat gtaataataa
    10501 tatgtaaatg agagattttc aaggcagaaa aaggtggcat aaggttttga gatccgacat
    10561 ctttttgttc gctgttttga tagtaaatat cattctcata aagagcgtgt ggagtgttta
    10621 caacaaagac gccgccgcca gactaaacag agaagaggcg gagaccacgt tggccaattt
    10681 acagaagaaa aaggccgggc ttgagaaaga gattgcaaga ttaaatacag acagaggggt
    10741 ggaggaggag cttagaagaa gatttcaggt tgtgaagcct ggagaacagg tgctcgttat
    10801 agtagataaa gaagagagta aggcgccggt aagttccgca aagccggagg gcgctaccga
    10861 acacatctgg aataaattgg taggtttgct atatttttgg cagaaatagg gttgtagatt
    10921 tggaggcgat attgtatttt aaaagggact tataaaaatg cgggtatggc ctgccccgta
    10981 ggaagttcaa ccacagaggc catgtttcat ttttaaggag gcgaagccgg ataccgaggg
    11041 cataaattta agttgccgac taggtctatc cgaactttcc ttctggggtt tagtggcctt
    11101 agtttatagt tcttttttat tttattttta ggacagagtt taatattatt gcgggtatgg
    11161 tttagtggta gaatgtgacc ttcccaaggt taagacgcga gttcgattct cgctacccgc
    11221 acaaaaataa ggaatcaaac gcaacgcgtt tgaggactat atttttgtag cgggtagcga
    11281 gaatcgaagg ccggaagcgg gcccctttct gcaagaaagg gagctgaggc ggggtcgcga
    11341 ccttttgcga agcaaaagag ttgtgaccga ttctcttatg gcgcgtttgt agaggaagac
    11401 actaagcaca aaatgtcaca aaaattccaa agaagaaaag aagattttgc ctgcgagaag
    11461 tgcggcaact tcgtggtggg aaccggatat acaaaccact gcccgaaatg cctttggggc
    11521 aagcatgtag atatttttcc gggagacagg gctaataagt gccagggtat gatgaagccg
    11581 atttcgataa ttaatgaaaa aggggacttc gtcttgatac acaagtgcga gaaatgcgga
    11641 gaggaaaaga gaaacagggc agccgcagac gacgatctgt cttgcatact tcactagatt
    11701 ttgaagttaa attgtgttaa tttgaggaac gttaagatgt cttgccctca tagttcaatg
    11761 gatagaacag ctcactccta acgagcaaat ccatgttcga ctcatggtgg gggcatccag
    11821 atcaaaattc cggctcttgc cggttttttg tttggcgtgc aaggatatgt taaaataagc
    11881 acatggaaga gaaattggac gagttgttaa aactcacaaa agaaaacaat aaaattcttc
    11941 gaggaatgag gagttctcag agatgggcaa gtttttttaa ttatatttat tatgccctta
    12001 tcatcggatc atttatcgga gcgtattact accttcaacc gatagttgat aaggcgatgc
    12061 agtcattgca gggcgtggct tccggaatac aggatgtgca gggggcggcc gccggtgcaa
    12121 gaaatatcaa catatctccc gacacaatag accagctcca aaagacactt aatctataaa
    12181 tatattgatt tttgtgtgat ttctggtaga tttatatcta cgcagaatta aagccgaggt
    12241 agctcagttg gtagagcata tccctgaaga ggatggggtc ggcggttcga gcccgcccct
    12301 cggcaccaag aaaaacgcac tttagtgcgt tttttgtttg ccgagggagc aaggcgcctg
    12361 cgcgccttgc gtgcgggctc gaaggccgca gcgttgcgtg agtttgagcg aagcgaaagg
    12421 cgaacgccgc gaggcggggc tgcgaaaaat ttccgttagg aaatttattt gtagccgagc
    12481 ccgccctaca gcaaaattat tagtgaccga gccacccccc aataaaaacg ccccgtttgg
    12541 ggcgttttga tttacattga ttttattttc aggctcttcg ttttgtcttt ttctatcttt
    12601 ggaagtttga tggttagaag tccgtgcttt tctaccgctt cggcgttgtc cgcgtccact
    12661 tcagagggga gcatgattgt tcttgtgaat gacccccaat aaagctcttt tgcaaaaaag
    12721 tctttctcgg acacctcgtg gtgtgaactt ctagctcctt ttattgtgat tatgtctcta
    12781 cctatggaga cctgtaaatc ttccggcttc actccggcca ccattgttct gacgattatc
    12841 tctgtgggtg tctgatacat atcgatggca agctcggcat cgtcttcctg ttttgtaata
    12901 gcagacactt cctcttgcaa atccaagctg tcgtctatct ctatcttatt tctggcatca
    12961 gtgctgccgg tcagcctttc aaaaaacgat gatttggagt ttttcataga atgattattt
    13021 ttttaagctt ttaatttttt cgcacaagag gagaagcgca atcgtgaata gccatagcgt
    13081 ggagaaaact gcgaaaatat ttcctccatt atctttaatt ataaaagcat taaagaggct
    13141 atgcaataca gtggctgtga gtaaacccaa aataaggctg attttcttgt gcgctttgtc
    13201 tttgtagaag tttagcgcaa tgaacgaacc tactgtggcg gatgtgagca ggtgaagcag
    13261 ggtcgctccg ataaatctca tgttggcggt aataagtgac attatggaaa atgtcggttt
    13321 gaccaaaaaa aagaaatttt ccgcggctgc aaatcctaag gcggccgtga tgaggtatat
    13381 tatggcgtct atcggctcat caaaatcttt tgtccgcagt gcgacgaagt aggcggtgaa
    13441 atattttacc aattcttcgg taagcggcca gatgatgaat atcagaagat attcgctgtt
    13501 tacacctttt gcgaaaaatt gctggagcgc aaatgccggg aaaacggaca aagttcccgc
    13561 aagaaaagtt tttagcagaa gactacgggg ttcgggattc tttctgtctt cgcgcagcca
    13621 aaaccacagc cagaacaatg ccggtaagat acccgctagc agagaccaaa agacgttgtc
    13681 gtatgttatg cttccggcca ttcaccaacc attataagat ttttagtctt atttccaaaa
    13741 tggttattca caatctccca aacttcctct gtaacaaaag gcataaacgg gtgtataagt
    13801 tttagagagg tctccagaac agtttttagc atccaacggg tttcgttcgc cttaccgccg
    13861 ttcagcttct cttttgaatc ttcaattatt ttgtcagcga atacgtgcca gaagtattgg
    13921 tatatttcct cggcggctat gtgaagcctg tcgttttcta tattcgccgt ggcctgtttt
    13981 gccacgttct tgagttcagc caggatagct tcgttctcgg gggagaactt ttccggtttt
    14041 tctcccagat tttcactgtt tacgttggaa agcacgaagc gagccacgtt ccagatcttg
    14101 ttggcgaaat gtttttgtcc ttttatctta tcttcgtaga ttctggtatc tgtcccgggt
    14161 cccgcaccaa tgacaagggc gattctggct gcatctgcgc catatttatc ggcgatgact
    14221 atggggtcga tgccgtttcc gagagacttg ctcatctttc ttccctgcgc atcgcgcacc
    14281 gttccgtgaa gataaacttt tttgaatggg atatcaccta gaatatatcc ggtcataagt
    14341 atcattctgg ctacccagaa gaataatatt tcgtatcctg tctccagaat gtccgtcggg
    14401 tgataaattc taaggtcttc tgttttctcc ggccatccaa gcacgctgaa ggtccaaagt
    14461 cccgacgaga accacgtgtc tagggtgtct gagtcttgcg tccagccttc tccttccgga
    14521 gcgtttatcc cgcaataaat ttcctccccc ttataccaaa cagggattct gtgtccaaac
    14581 catatttgtc tgctgatgca ccagtcgcgc aagttctcta tccagtggaa gtatgttttc
    14641 ttgaaatgat ccgggacgat gtttatttgg ccgttctcaa cggttttctt cattatctct
    14701 ttgagtgtcg tatcggagcc ggaggggatg ccgtctattt tagaatcggc aagagtaaat
    14761 tctttgttaa cattgaggaa ccactgcagt tttggtaatg gctcgattat tccgcccgtt
    14821 ctttctgcag tagaaatatt ctggctaact tcttcctctt tctccaaaag accttcttgc
    14881 cttagccatt cgactacgat ctctctggct tctgttgttt tctttccttt aagcagggga
    14941 gcatcaacca tcattttcgc gtattcattg atgacttgcg gacgaggaag gtcgtgtctt
    15001 tcggcgatat cccagtctgt catgctatgg gccggagtaa ctccaagcgc gcccgttccg
    15061 aattccttgt ctaccgtttc gtcggcgaca atctttacgt gtatatgaac accggcgaaa
    15121 ttagcatcat attcttttcc gacaaatttc ttatatcttg gatcgtccgg atgaacagct
    15181 actgcggtat caccgacttt tgtttccggc ctcgttgtgg atatggaaat agggaaatct
    15241 tttgaatact tgaatgtgta gaacttagcc tttcgctctt cgtaaacgac ctcatcatcg
    15301 gatacagttg tttgtccttt tggatcccag tttacgatcc tgtggcctct atatatgagc
    15361 ccctcatcat acattttttt gaaagctgtc ctgacggcga gatttctttt ctcatcaagg
    15421 gtgtatgcct ctctgctcca gtcgacagaa acgcccatag tcttcatctg gtttactatt
    15481 gtatcgtgcg agtcttgcgc gaatttttct acgcgtttca aaaattcctc acgtcccaga
    15541 tcgtgtctgt ttttgccctc ttctttatat attatttttt ctacttttga ttgtgtggca
    15601 atggcggcat ggtctgtgcc cggaacccag agcgttctct tgcctctcat tctgttaaat
    15661 cttaccaaga tatcttcgac ggtaagcata agcgcgtgac ccatgtgaag agttcctgta
    15721 acgttcggag gaggaagcac catagagaaa accggtgcat cagctttagt gacgcccttt
    15781 tttatacact cgtcgggatt gaaaagaccg ctgtcttccc aaattttata aatttttgac
    15841 tcggtgtttt ttgattcata aggctttaga agtcgttcgt caatatttgg ttgtttgttt
    15901 tccatgggtt aaataatatc aaaaacgcgg ctatttaaga agcaggttgc cttctgcttt
    15961 tatcctttta attaaaggta ggctgaacac cggttttttc ttttgcgcgc ggaagtatcc
    16021 ggctatgcct atcattaccg cattatccac ggacaaggag ctttccggta taagtagctc
    16081 tgtgtcggca agatcttttt taatcatttc cgttaaagag acgcgaagtt ttttgtttgc
    16141 agagacgcct cctcctatta ctagtgactt cgccttaaat ttatttatgg ccttttttgt
    16201 ttttgaaacc agtgtatcta ttaccgcttg ttcaaattcg aaagcaaact ctttcttaag
    16261 ctcgtcggta agctccgcca actcttttgc tttataaagc acggatgttt ttagtccgga
    16321 gaaagaaaaa tcaaagtcgt ctgaatttat catcgggcga gggaatttta ttccggttga
    16381 atttttaata tcaaaagttt ccgcgagttt ggatattttc ggtccacccg ggtaggtgag
    16441 gccgagtatt ctcgcaacct tatcgaacgc ttcacccgcg gcgtcgtctc ttgtgctacc
    16501 gagtattttg tatttcatcc agtctttcat aagcaccagt tccgtgtgtc cgccgcttac
    16561 aagcagggcg atggacggga attttattcc tctcgttttt ttctctgcaa gcaggcttga
    16621 tagcgcgtgg ccctccatgt gatttaccgg gacgatcggt tttttccagc aaagagaaag
    16681 cgctcttgcc atgtttacgc caacccatag tgctggctca agtcccggac cgtaagttac
    16741 ggcgatggca tctattttag ggatctctat ctcgggaagt aaaagttcca tgtttttaag
    16801 tagctccggt tctttttcta atatcttatg aagttctcct ggaagagttt tttctttcgc
    16861 ggccgttttt aaaaggcctg ctttttccag tgatgcctca agaaggggaa ccaggtttat
    16921 agaatgttct ctctttgcta aaaaggggac cactccctgc cattttgcat gaagttctat
    16981 ctgggaatgg gtttggctgg atagcacctt aaaagaaggc tttttaagcc ctccattggc
    17041 ttctattatt gctatggctg tatcgtcaca gcttgtttct atggccaaaa ttttcattga
    17101 tttattggat aaaatctagc agattttggc aaattttgaa agaagagggg gctacctagt
    17161 ctttgttgct tttgttgcaa ttttagtgtt atcttgtgtg gagacaataa tttttaaaag
    17221 gggtgaggtt atatgtttat tagacctttg aaaatccagg atgaatctca ggtggttgca
    17281 ttgatggaga atttcctttt ccttaccaag aaagaggtgc tctttaacgc ggctgagtgc
    17341 ttgaacgata gtaacgttca cgggcttgtg attgaggaca acgggaacgt cgtcggacac
    17401 ggggccctgg ttgtgtctcg tattcccagc aagggactct acggcattgt ccacgatgtt
    17461 gtcgttagcg atcagtatcg cggacagggt ctcggcagaa agcttgtgaa tgaacttgtc
    17521 gatctggcgc gttctctcaa gctgccgctt attgtcctca cttcgaataa ggctcgcgtc
    17581 gccgcgagaa atctctatga gtcgatcggt tttgtcgaga aggataccgg attcttcgag
    17641 atggtgttgt aatgttttgg ccgcacaatg tgcggccttt tttgttgggg ggggcgacac
    17701 aggattttcg cctacggcga cagtatgggt ttccgatatt ttagaaggaa tcgctcacaa
    17761 gttactaaga tttttatttc gccttgctta taaaaatcaa agtacttgcg agcccgcctc
    17821 gtctcccttt cttgcagaaa ggggcccgac tgcggccttc gattcctgtg tcgctcctac
    17881 aaaacgaaaa ccacccaatt gcttacgcaa ttgggtggtc gttttgtggg cgacacagga
    17941 atcgaacctg tgacctttac aatgtcaatg taacgctcta accatctgag ctagtcgccc
    18001 tttaacttaa acatttaacc ccttttttat atttttcgca agtctacttt tttgctttct
    18061 ttgttggctt tttctccctc atacactata tagtctactg agttgagagt gccgctatca
    18121 acgtcttcca ttcttctcat atatgattga ccgcttttgt tgactgaggt tgttatgaat
    18181 gggacaccgg ccttttgtat aatttttgta aaccagtgtt tggggatacg aacgcctaaa
    18241 gaatcgtctg ccggattaac ttcttcgctt acacaggccg tgttttttct gtccaaaaat
    18301 agcgttaatg gaccgggtaa tttttttgtc cattcttcta tttttttatt aaaaacaaaa
    18361 ttatctttta tccatttttt atttggggct attacagaaa acggcttaag ctccctttct
    18421 ttaatcgttc ttatttttgc tacagaggtg ctcttttttg cattagcgcc gataccgtaa
    18481 atggtgtcgg tagggtatat gaatatcttg ccggctttaa tttcccgaat tattttttct
    18541 tcattatttt ttatatactt tttgtcgata attttcatat atcgaataga tcattagaaa
    18601 ataacacaca cttattgacc gcgcaaggtt tattgattat caatcgtttg tgttagaata
    18661 tatataatta atttttgtat atgaaagttt taattgtaga agatgatcct ttgctggtag
    18721 aaatggtaaa ggctaaatta aagaaggacg gttacgaagt tttttccgcc tttaacgggg
    18781 aagagggtct gtgccgtgtc aaagaatgtt ttcctgacgt gataattctt gatatcaata
    18841 tgccgataat gaacggattt gaacttctta aaatattaaa gaaagacaac gttttccaaa
    18901 aaatacccgt tattttattc tcaagcacgg gagacaaaga agacaatctt gaaatgggca
    18961 agcggcttgg agctagcggg tttttggtaa aatctcagct gtctctgaac gatatacccg
    19021 cgaaaataaa agaggttatc gggcaaaaat aagaaaaata atatggcggg gattatcata
    19081 accccgcaaa agagcatctc cgggcgctca ttgaccggct gaaggttttt gccgcaaact
    19141 tcagaaggtc ggagattgcg gtggaatgga aacccggtgg cggcatatga gggaaatttg
    19201 gggatttaga taaaagaaac ccctcggatg ggaggggtta tgtttatgaa tactcttttc
    19261 tcttttggtg tataacagat atggcgtgga taaccacttg attcatgtct agtaattcca
    19321 aagttacttc tgattctatt tctttagaca aattataata ttggtcagtt ttttcttcta
    19381 agttcttgat tgtcataaga acctttgccc gtatttcttc gggcctagta tcttttcgtg
    19441 aaaaagtccg attgaagaga tctatgattt tcaatatttt ggtaatttct tcgtatgttt
    19501 cttttaaaag aataatcttt cgatgttctt tattggctgt aattgtttta tccacgatat
    19561 cctccttttc aaatatcaaa atctaaccaa aggctactta ttttaaagat gaatgtcaag
    19621 catgacccta cagagaatcg gggtcaagtt tctaattcac ttcgttcata agaacttcct
    19681 ccccggactc gctgacaagc actcgctttg ctcgtcttgt ataccagctc cgtctttcga
    19741 ttctctgccg ggacccgtta aaacaaaatc accccgacct ttcagtcgag gttcatttgt
    19801 tttatgaccc tacagagaat cggggtcaag tttctaattc acttcgttca taagaacttc
    19861 ctccccggac tcgctgacaa gcactcgctt tgctcgtctt gtataccagc tccgtctttc
    19921 gattctctgc cgggacccgt taaaacaaaa tcaccccgac ctttcagtcg aggttcattt
    19981 gttttatgaa cccattgcgt ggctttcaga actcgctaaa caaagggatt atatcagttt
    20041 agctattgga gatctaaaat tagctatgat tactgcctaa ttttttctta ttgtctttga
    20101 atgcttgaat aaaatacctt tgaagaaaag aaaggatgct tttcatatcc ttattaaaac
    20161 ttctcactcc ttctagggta aaaatggatt tgcctttgta tgtaaccctt tcagttccta
    20221 gattttctgg tatgagtgcg tgggccatag cgtttctcaa gtcattgagt ttatttaggt
    20281 cggtgactat ttttttggga acaaagataa agcttttaat acttgatacc tttcttaaaa
    20341 aaggcatttc attaagtatc ttttctgaaa atacgataaa ctcctttgat gtggttatct
    20401 tttgaacttc ttcacaatca tcttggcaat gatgaaagaa gaaattagct atcagtatat
    20461 tcatcttagt gtctatttgc agataagtct ccagcaccat cattcgtatt agtttttgtc
    20521 tttgtccacc aaggtgagcg aaagtataga tattatcact gcctgtatca gtaagctcat
    20581 ttaaactagc aatttttaat agttcctgta tgtctttctg ttcttttttt attctttttt
    20641 ctcttttgtt cataattttg atgattttgt ttcttgtctt cttgtatcaa ccaagagaaa
    20701 accccaacaa ccctgtctct ttcttcatta gtccattcat ttcgtctatt atcttttact
    20761 tccatctatt tcttttactt ttttccaatc aagttcttct acattgccgt ttttatcaac
    20821 aaaaaataga tatagattta atttatccat tacgtcgaat ggaattttgc gtatagccat
    20881 ccttttaaaa gaggaaggaa gggcaattcc gtatttgtat ccatatttat aacctctagc
    20941 cccattacgg tgcattctcg ttacaatctg ccccagcgct gagttaaaac tactactctg
    21001 gctcccagac gggcttagga cttttataga gggatctccc tttgcctcaa caagccaata
    21061 tctcgaaaat ttattattac agacttttat atcaactccc tgctcccaga gctccttgct
    21121 ctttagcgac ttactccatc catttttctc aagatgcctt ataaccctat cctgaacaaa
    21181 atcttcggtt attataattc tatctttttt ggctttcttt ttggtcatac tgtttactgt
    21241 tcttattttg taaacaatct agttaactca tcaaagtttt tggatggatt tttttcgtaa
    21301 tcttcttgtt ttacatagag aggtttatac acaactcgac tttgccttga atttacatct
    21361 tcacaccact tctgtaatcg ttcaaactta attttatcat cttcttcctc tcgtcctttt
    21421 gtttcgatga tgtagacttc tttttcatcc tttttaacga agaaatcggg ataataaata
    21481 gcaataaacc cctcggcatt tttatactca atacggagag cactcgcttc cttattttgg
    21541 aagttcttgg caaaagaaat tatgtcatcg catttttcaa gaaaagcgga aaactccaat
    21601 tcaaaattac tatctccaac aattctgtta aagactgatt tctgtggaat aagaaatgac
    21661 ttatcgttca caacgaatgg tcgtgcatcg ctaatcttta tatagttctt tatttcagta
    21721 tctcccttat cttggaccgt gagttcattt atagatttct tgaacccgtc ctttatgagt
    21781 ttaatatatt caacttcgga taggtttcgg agaacattaa gatccgtcaa ttcaaccttg
    21841 ctctcaaaca tataattctc gacgaagtct ttcaccttac cgaaaagaat gtcatagcac
    21901 ccgaacaacc tcatctccct cattatcgct tgagcaaaaa atccaaccac actttgatag
    21961 tttggttcaa tctcgccact taaaatagta gtgtggtgaa cctcttcagc aacaacatct
    22021 ttaaagacaa tttctcgttt ttcctgttcg gaaaagattt ttacttttac ttttttgttt
    22081 ccaaaacttg aaacatctaa ttcggctaga tttttatatt ctctctgtat tcttgccgtc
    22141 attacgggga tttcgatatc aagcttccca atgtctttct tagcgttttc tttatcaatt
    22201 tcaataacca ttggagagat aggtttcgag ccagaaccca taagcctctt ttcgaggagc
    22261 acaccttctc cttttatgct ttcaacgaaa tccataaaag caggagtgcc gatgacgctt
    22321 acatattcat ctatatcgtc tcttccaaaa tacattcttc gcagacctct accaagggtt
    22381 tgttctggta atatcttact atctgaggcg tagggtcgaa gtccaacaat agtcgtcaca
    22441 ttcttcacat cccaaccttc tttgagcatc attactgaaa caataacctt atatggactt
    22501 tcccaactat caatctcgtt tgattgcttt cttagctgtt ctagttcctc tttatttctg
    22561 ccagtgacgg tttcagaaat ctcaccgctc ttgtttgtgt gaatggtcaa aacagatcct
    22621 tttaaatcag gaaaacttgt ctggagatag tcagcaacct catcacaatt ctttgtgtca
    22681 tcagtcatta taaaaagaat tgatttcttt ccagtaggtt caagttgttt gtatgttttt
    22741 ctccactcct caacaccaag attgatataa tctcgatatt tctcgctaaa taaagtgctc
    22801 tgatttggtt tcagcttccc tcggcttgct tcgtcgggga ccacgggatt ttttaccacc
    22861 ctctgatgaa ttgcctcaac taaaggatag tccgagataa cttgaacaaa aatagagcca
    22921 tcttttttct ttggagtagc ggtaacatct atttgaagag caagtccacc acctttttgt
    22981 ttaagcttgt tgtctatgtc acgtatggat ttgagccacg cattttcttc gtgaatatgg
    23041 tgagcttcat cgttaataac gataagttca ttaatatcac ggaccaccat ccctaaatca
    23101 accattgcat catttgtttt tgtgactggc ttattgccaa gaaaataagc ggaagtgtct
    23161 tcatcttctg cactgtaatc ttgaacttca ctttcaaaaa cacgatgaat gttggtgaga
    23221 aatatatttc ctgtatcaga aacatttttt agttcgtcct gcaaatgaag tgtgacctga
    23281 aaatcatctt gccaattctg acccatatag ccattgtcgg gaaggacggg atccaagaag
    23341 aatatcttat ttccttcaaa gtcgttctta atacgttcaa atacgatgac gtttggagca
    23401 ataagtagaa agttctttgc gagttctgag cccttctcgt ataacttatg aaaatatgcc
    23461 caagcgataa ccaagctcat tactttggtc ttaccagccc ccgtagccat ttttataaca
    23521 aatcgtagcc agtcctcagt aaacatctga gggcttaaaa cacccgtgga gttataacgt
    23581 attagatcat acttatcctt cactcctgcg acctcgtaga gccacacaac agtttccagg
    23641 gcctctctct gggagaagta atatcggaaa ttataggaag aaccgtcttt attatataga
    23701 gtgtgctctt cttggaacca ccaagttaaa agcgcccttg aagtctcgct tgcatctttg
    23761 taattatgat ctctccaatc ctttacttgt ttgcgaagct cagcgacaag agggggaatg
    23821 agtttatcaa atcctttttc tcgtaaatcc tcatcagcgg gaaaccacct gatttcaggg
    23881 ttgagaattg cgtatgggtc tcgtggaaag tctttgtgta gtgccattat tttatttgaa
    23941 attatatctg tggataactt tgactttttt aaaaaaattt gctatatatt aattgatcgt
    24001 aagatcacta attttagtgc ttaggggctc cgcaaggggt ccctttggct tttataaggc
    24061 caatttatct ttaactgcaa ggggtatttc ttttggaaaa caaacaaatt ccttaatctt
    24121 tttaacatca aaaataaaga cacctgtttc gtctagttca ggagcaacct tccctgaaac
    24181 agcaaataga attgccctct ttccgctatt ttttatttga tttatcggac tagcaaagtc
    24241 actgtcaccg ctccacaaaa caaatgattt tgtattgttt ctctcgcagt ctaagagcat
    24301 atctaccccc atttctacgt caaaattaca tttgggttcc tcgatatagg taatgccttg
    24361 tttgtttagt aaagacatct ttttattcaa atacttaacc gtatcaatat ctagttttga
    24421 aagaaatctt tttttcataa aattcttcaa aatcgcaggt gaatagtcgg gaatactggt
    24481 aacatcgata gagattttca tcatttttac tggctttgta gataagatgt atttacagtt
    24541 ctctatttct tttatttgct caagggaacg ttcgtctgtt tcaagggtgc ctgtataaat
    24601 tattaccgat tgtatcgtat caaaagaatt gaaaaattgt tttagtcttt ttaggctgaa
    24661 atgccagcca agtttttctt gccaatggag cacattttgc caatcaatat aaatttttgt
    24721 gttttcatta aaaatactat ctaactcctc aattaccttg gggaatagtt ttgctatttt
    24781 ttctattttt ggtgtgtaag gtttgaacat ttttctaatt tatcttttgg tatttgtagt
    24841 tgtatcgtat gaaatcattt aaaaattggc cgtgagatga gctgtgcaga aattgctgga
    24901 acaagtattt tgggatgttc aaataatgat aaatatcccc agaggtgaat tcgacctcta
    24961 acgtgctagt tacctcatcg tatcctattg aattaatatt agacgaaacc acagatattc
    25021 tgttcataaa atttatattt ttacatcaat tactctggtc gtatcgttgc cgaaaatatc
    25081 cacgaccttt accgcaacct tgtaatctcc cttgggaagt tcttttgggg cagacttgaa
    25141 ctctagattt cgatttttct tcgtgcggaa actttgccat tcattatcaa agatatagtc
    25201 tccagtccaa acttcttttt ctttgtcatt ttcaataacc tttatgattt cctttctgtc
    25261 cgcaaagtta tagtcaactg cccagtaatc aatccagtcg ctccatttct ttgtgagcac
    25321 ctctcttgat actagttccg tgtctttatc cttcgttact ttgattactt gtccattttc
    25381 gatgattact tttgatctac cagcctttaa ttcttcctca acggcgccat tatcctgatt
    25441 gtaaaataca gagaagtcag caagctcaat tgaaagttcc ttcatatttc ctctacctgt
    25501 tactatcggt tttgcctcaa tataagcaac atcatagaag ttcacctggc cccgctcaac
    25561 ggctcgttta tcaaaaactt cacgagggat aattttgaaa gcgatatcca ttccttgtgt
    25621 tacgaactcg tcaaaaatta gacccatttc ataatcaaaa ccaagcacat caaccttcgt
    25681 gatattcttt tcttttaact ctttaacaat ctcttgtatg aatttctggg atactgggag
    25741 gttgattgga ccaatggcga caagtctgtc tcttttcttt ccgacaacat tcacaaaact
    25801 atcaactgct tcggctttgt atgcagaaag gataagcttg ataaaatctt tttctttacg
    25861 ttctgcttgt tttgcttttt cttctgcacg caagtcattg ttagtatcga ggaaactttc
    25921 acgttcgtat ctgccaacgt tcaaaatctc aaacgcacga aaatcttttc ctgctttctt
    25981 aagttctctt tgtattccaa tcattcgttt tctggatgta tgtataccaa acttacctaa
    26041 gtcagaacct atccatttac gaccaagttt ttcggcaacg gccaaagttg ttccagaacc
    26101 acagaaaaaa tcagcaacca aatctccttc gtttgatgag gcatttatta ttctctcaat
    26161 aagactttct ggcttttgag ttggataatc taagatttcg ctataatcac cttgatttgc
    26221 cttgaatgct ctagggtcag catatatttg ttttatatct ttccatacag ttgatgctgg
    26281 ttcaccaggg ctctcgtcaa gatattgtct ttcatatatt ggctttccgt ctgaactttt
    26341 tcctcttatt ctttgacgat aaactctgcc attctcatct gtatatttga accaatcttt
    26401 taaatatttt tctgcgtatg gtaagtatat tttattttca atacgattat catagttttt
    26461 cgcaaaatgt agaatgtatt cgtgtatctt taccaacttt gttccagaag cacgaccacc
    26521 agaaggggtt ccgtatgacc agataatttc tgaaacgaga ttattctttc caaaaatctc
    26581 ttccatcgcc aatcggacat aatgattgac ttgtattcca cagtgcacat agatgcttcc
    26641 attttcggca agaagttcgt gcatcaattt caagcgctcg taaatcatcg ctacataact
    26701 atctacaccg ttaccccaag tatccctaaa cgcaacctct tctataactg atggtttctt
    26761 tgtaaaactt tcaccatctt ctttgtcacc aatagttata tccattgaaa aatctgctcc
    26821 gacgtcaaag ggggggtcga tatagattag tttaagacca ccttgagcct ctatttcctt
    26881 tctcattggg ccgtttttaa gggaggaaag aatgagttta ttgtctcccc aaatgagttt
    26941 attggtccat ccagcaacct gtttaccaga gaaatcaaac agtgtgcccc taccatcacc
    27001 gaactctttt ccttctcggg gttcatcaat ttgctcaatt acctggaagg gaagaactac
    27061 atttgttact tcgttagact ttccattcca aacaagttct acttcatttt tgtctccaaa
    27121 taaaagaaag cgatatttat ctggtagcgg tttattcgct tccagaaact ttgtaatatc
    27181 tctcttttca ttatctgata aaaaaatctg gttattttta ctattggtca tttgatagca
    27241 ttctacacga catttgagtt gttctcaatg ttttatttaa gattatcgag ctacttcttg
    27301 g
//
LOCUS       DAOTEX010000004        21566 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3118
            NODE_1603_length_21566_cov_4.133374, whole genome shotgun sequence.
ACCESSION   DAOTEX010000004 DAOTEX010000000
VERSION     DAOTEX010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868142
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 21566)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21566)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 883
            CDSs (total)                      :: 833
            Genes (coding)                    :: 829
            CDSs (with protein)               :: 829
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21566
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1603_length_21566_cov_4.133374"
                     /isolate="AMR_MDS_3118"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..698
                     /locus_tag="P5056_00490"
     CDS             <1..698
                     /locus_tag="P5056_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62242.1"
                     /translation="CFGSKMKTEARKKAVEMRKAGASYGEIKRRLNVSKSTLSYWLRE
                     MPLAPKRLLELRRKGWSKGEASREKYRETMRRKKEDRSRKIYDKYAERFSRLSDDSIF
                     VAGLMLYLAEGAKKKESTIVIANTDSGVIKFFMRWLEKYFGIDRSEFRIQLHLYEDMD
                     LNKERNFWKKELVIGSNQIYKLSVRKLRPASFTYKDSFRHGTCSLYAFGVDRVVEIMS
                     AMRAFVDTVNKNK"
     gene            715..787
                     /locus_tag="P5056_00495"
     tRNA            715..787
                     /locus_tag="P5056_00495"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:748..750,aa:Ile,seq:gat)
     gene            874..1428
                     /locus_tag="P5056_00500"
     CDS             874..1428
                     /locus_tag="P5056_00500"
                     /inference="COORDINATES: protein motif:HMM:NF015944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HRY62243.1"
                     /translation="MNKTLWIILGVALVVLGYVWMTYNGLVTGSALVDNQWKQVEVQY
                     QRRLDLIPNLVESVKGIMKQETATLTLVTDARTKYMNAATTGDKVTAANELESAVGKF
                     LVLAENYPQLRSVEAVLSMQAELAGTENRVAVERMRYNDNVTSFNLMTKRFPSNMIAS
                     MFGFEQAVYFNAEKGAENVPKVQL"
     gene            1437..2315
                     /locus_tag="P5056_00505"
     CDS             1437..2315
                     /locus_tag="P5056_00505"
                     /inference="COORDINATES: protein motif:HMM:NF016424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HRY62244.1"
                     /translation="MKILKIFGAIIIFASLTSVVSAYYSLGRPAGFVYDGAGVLSAEQ
                     KTALESKLSLFEKETSNEIAIAVIPNLGGDYIENFAIKLFEEWKPGKANKDNGVLILV
                     AIEDREMRIEVGYGLEGALTDSQANWIIRNVMTPAFRDGNYSVGIDGAVDKIIGATKG
                     EYVPDDNGPADSTAFENIFPLIFFVLFLVLDVLIMVFGRSKSYWEGGVFGLVVGALIG
                     IIFVSYFAGFLLAFIFGVLGLAFDYFVSRVVDPEKIRRHIGGGGFGGHGGFGGGGGFG
                     GFGGGSSGGGGSSGKW"
     gene            2437..2619
                     /locus_tag="P5056_00510"
     CDS             2437..2619
                     /locus_tag="P5056_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62245.1"
                     /translation="MKDNREQNILDRIRDEKFTDDEIFREVSSGLYSEDQNAVIHVLQ
                     NYRPCLLKQLLCFVEE"
     gene            2628..2837
                     /locus_tag="P5056_00515"
     CDS             2628..2837
                     /locus_tag="P5056_00515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62246.1"
                     /translation="MPKLEQNEMIFQGIGIPIETMITDPRVPDDVLRLEFEPLQHHGH
                     VLFLMKAHRPKLADELFHTFDFSKN"
     gene            3177..4970
                     /gene="typA"
                     /locus_tag="P5056_00520"
     CDS             3177..4970
                     /gene="typA"
                     /locus_tag="P5056_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003515569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HRY62247.1"
                     /translation="MNEDIRNIAIIAHVDHGKTTLVDALLRQAKTNIKDEVLNTECIM
                     DSNEIEKERGITIFAKNASVRYGNTKINILDTPGHADFGGEVERVLTMADAALLLIDA
                     KEGPMPQTRFVLKKALQLGLKIIVVINKMDKPEARPAYVLDEALNLFIELGANDAQLD
                     FPVIYSISKQGKAGMKPDVSEMKDITPIFETILKFVPPAERDVDANLQFLTVNISRDD
                     FKGRIATGKIYKGKIKSGETVSCIGRDGKAVNFKVTALMVSDGLGRVEVCEAGAGDIV
                     HIAGYPDIGIGDTISSKDNPVALTRLKIDEPTIQVVFMVNNSPFAGREGTYVTSRQLR
                     ERLYKEMETDVALRVEDTNFPEKFLVSGRGELHIGILIERMRREGYEFQVGRPKVIYK
                     DIDGVKNEPYEEVSIEVPDNFAGTIIEKMGTRGAEMKDMRLHEGITHLTFIIPTSRFL
                     GFRSKFMTDTKGLGILNSLFYGYLPAVEARPQPHGSVVAYEDGQTTAFALENAQLRGT
                     MFVPPGVQVYRGQVVGRCSRDEDLEINVCKKKELSNMRSTSSDGMVMLDPHVEMGVED
                     ALEYISDDELVEVTPKSVRMRSLKVFKTKGH"
     gene            5267..5521
                     /locus_tag="P5056_00525"
     CDS             5267..5521
                     /locus_tag="P5056_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62248.1"
                     /translation="MISWLLLPFNFLFSVLWWICFIVLNGIVLAAILFIICLPFILMY
                     AASKVPDRMTHAEKRYRDYMLYEKYHEPPPDYEEPEDKTN"
     gene            5626..5814
                     /locus_tag="P5056_00530"
     CDS             5626..5814
                     /locus_tag="P5056_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62249.1"
                     /translation="MFFSGTVLGFGFLGWFGWIFFPRITIAVLATNSYWDTNPVLVVI
                     AWMIAFTSESKEKKALHR"
     gene            complement(5999..6871)
                     /gene="dprA"
                     /locus_tag="P5056_00535"
     CDS             complement(5999..6871)
                     /gene="dprA"
                     /locus_tag="P5056_00535"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HRY62250.1"
                     /translation="MKDSIRKLNQNEFPKALLEIPQPPKQLYVEGELPDENEYIYLTV
                     VGSRKFSNYGKDACEKLISELAGYNIVIVSGLAHGIDAIAHKAAMKAGLKTVGVPGSG
                     LDRKVLYPSLNRKIADEIVSKGGALLSEFDPMQRATRYTFPQRNRIMAGLAKAILVIE
                     AGDKSGTLITARLATEYNRDLLVVPGSIFSPNAAGSNKLINLGATPVSSGEEILKALG
                     IDVETKQKALDLKDPALSENERKVLEILSIEPLPRDDLIRELKLPISESNTLLSVMEI
                     KGLITESLGEVRMN"
     gene            7176..8453
                     /gene="serS"
                     /locus_tag="P5056_00540"
     CDS             7176..8453
                     /gene="serS"
                     /locus_tag="P5056_00540"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HRY62251.1"
                     /translation="MLDIKFIKENPDLIKEAARKKHSNFKVEELIEADGDRVALIKEV
                     DEIRAKQNAMNPKISSTSDMKERNDLIFQMKEIKDELQKKEARLEEVMKKWRSLMVQV
                     PNVPDMSVPEGASDADNKEIKTWGNIAKFDFTPKSQHEILLDLDMVDFDRGIKVSGFR
                     GYFLKNDGFLLSMAIWQFCIDSLVKKGYSPLLAPSLVKKEPFYGTGYLPQGEEDLYKT
                     QDADYLAGTAEVGTMGFYMDEIVPKEILPKKFVSFSPCFRREAGSHGKDTKGLIRLHE
                     FYKVEQVILCEASHEQSVKLHEELRENAEEIMQKLGIPYRVVINCGGDLGLGQVKKYD
                     IEAWVPSEGKYRETHSISYFHDFQTRRLNTKYKDEDGKLKFAHSLNGTALATPRALVA
                     IVENFQNADGSITIPEVLRPYMGGRTKIEKPQV"
     gene            8760..9437
                     /locus_tag="P5056_00545"
     CDS             8760..9437
                     /locus_tag="P5056_00545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62252.1"
                     /translation="MIDFLKTNILKIEEAEVSLDDLNTLSINITERQARLLWCKNDPK
                     TCYYADGNGLIFDRAPHFSDGVFLRFEGGLPSEESDSYYVGKNIFPAEYIKMLITFRQ
                     RAEELLKKNFSDNWKASYYFLGEGSDIDLFFENGIGGKWKIMMSPRTEEGYSFKGNDS
                     SGLLKPETEATWAGFGGDLESAEANLKVILSSEAFRGSADNKTEPLKNLEYIDLRFGK
                     KIFYKFN"
     gene            9599..10258
                     /locus_tag="P5056_00550"
     CDS             9599..10258
                     /locus_tag="P5056_00550"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HRY62253.1"
                     /translation="MLTLTAKKRDTLGKKVKSLRYEDLIPVVVYGPGEPTAHYSVKLN
                     EFIKVWKKAGETVVVAIETDSDGVKNTLIQDVVVNPITGTPIHADFYAVKKGMTVSVN
                     IPLRFEGDAPAIKAFGGILVRVMHNVEVEAEPDKLPQEIVVNVSSLETLSDRVTVKDL
                     KLGEGVRAVTSPDEVIALVSAVVEKEEKVEEPADLSKIEVEKKGKKPEEGEEGAEPAK
                     K"
     gene            10369..11763
                     /locus_tag="P5056_00555"
     CDS             10369..11763
                     /locus_tag="P5056_00555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62254.1"
                     /translation="MNLSTSLKTKKTIFLGLLFLTFFPVVSFAEDTSISCDTVNENER
                     PEVLDKKFLKCQEEILAHQQLIQEKEKESASLQRDLKLITAKVEKTKSEIKARNIAIV
                     NIEREIDKKNQNIEEMNVKMDDIRTSTAELIRETEDLESITLPEVILDNRNISEFFND
                     LASFESVEASLKETLNKYKDIQKSEEEAKKDLAGKKEKERGLKSLQEIEKRKTELAEA
                     EKARILKETKGEEIKYKAMLAEKQALVNKIKNMMLKLTGGGELKFAEALRLVRVAEQA
                     IGVRAAFVLSILTQESGMDGAIGANLGKCFYNTPWNNKSGTVMSDTQKPAYLALLSEI
                     GKNPNTTPVSCPISKDGQYGGAMGPSQFMPKTWWDGAAGTGYKKRVEKVTGNVPASPF
                     NNLDAFTATALYLSDALEGCEKTYTSKYNQEKCAAAKYYAGANWKKHLNGYGAKVANR
                     AVELQKDIDVLDSQ"
     gene            11786..12010
                     /gene="rpmE"
                     /locus_tag="P5056_00560"
     CDS             11786..12010
                     /gene="rpmE"
                     /locus_tag="P5056_00560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015725140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HRY62255.1"
                     /translation="MKDKIHPKYNDKAKVICSCGNQFEVGSVLPEIRVEICGKCHPFF
                     TGNERIVDTAGRVEKFKARAAKKTGTKKEK"
     gene            12116..13102
                     /gene="prfA"
                     /locus_tag="P5056_00565"
     CDS             12116..13102
                     /gene="prfA"
                     /locus_tag="P5056_00565"
                     /inference="COORDINATES: protein motif:HMM:NF001859.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HRY62256.1"
                     /translation="MDEKLLKLKDNPKTTYLFKGYERILAEIEENQKIVESDPELKEI
                     AEADIKKLEESQSSLLSQMEEIAKEEEAAALDPKEMILEVRAGAGGDEAAIFARELAE
                     AYMRYAELKGWAWKPIDESESELGGYKEASFEIRGKGAYSHLKYEMGVHRVQRVPATE
                     KQGRVHTSTASVAVLPIVESKKIAIPESDIEMEFSRAGGKGGQNVNKVETAVRLVHKP
                     TGIMVRCTSERSQLKNRETAMSILAAKVAELKRVEEEKKLSSERKSQIGTGDRSEKIR
                     TYNFPQNRITDHRIKESWHNIEKVMAGDLDPIIDALIAFDKGERTVGSSDEE"
     gene            13219..14781
                     /locus_tag="P5056_00570"
     CDS             13219..14781
                     /locus_tag="P5056_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62257.1"
                     /translation="MSHKKHLLLLTLLSIVFAPGLYFSHNDPIPAIAQSGSNKLSGWA
                     WSSNVGWISFNSDDCTTSANFINNACGGNGIKKLVDSSGTPITYGVTLDSSNNLVGYA
                     WNPDLGYIYFGGDSDNAGGIESAPEAPKTWARLSGNAVNSQITGWARACSVFASGCNG
                     ALKSNIQRGDWDGWIKMSDDAGTIKYGAYIKDIATNGQKIFGGFAWGGGSNPAMSSSA
                     QFPGWINFDYKTNPDGTPVTPGCVGQSCQAGCTENCGGVVVPPSVGPETFFVPRCLSE
                     LAEGVTVVEPNKTLTFSVNPEGPGNNDNYTIRWFDDGVEKPGQTTRSYTLTTSASVPS
                     HNITVSVVPNPAGGVAVRTLSCTPGGLTVAASPTASLIAFSDLNLMNMSVILPEGAIP
                     VTAKNISGVLPKITIIDATGSPCSAQITLSVEKITKNGVPIPEVHWKKSTAPNQSPVF
                     SPKNLISVGSSSNLTLNIIRQPAGTTRLSDGTYSVNIKASPAGGACGGDRYRDAALKI
                     SSTGGGGTYIEE"
     gene            14981..15763
                     /gene="rpsB"
                     /locus_tag="P5056_00575"
     CDS             14981..15763
                     /gene="rpsB"
                     /locus_tag="P5056_00575"
                     /inference="COORDINATES: protein motif:HMM:TIGR01011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HRY62258.1"
                     /translation="MAEIEQKLDFSGLEDKSLAALVEELHKAGAHFGYSRSRRHPSVK
                     NYVFGSKNRMDIIDLEPMAKSLKAALEFVGRLGAEGKQILFVGNKKEAQDAVKAGAEK
                     LNMPWVALRWMGGTLTNAPEIRKRVARMEDLIAKKAVGGLDMYTKKERLLLDREVAKL
                     QKHFGGVVSMKRAPAAVVVVDSRNEETAIAEAKMANIPVISLSGTDNNIAGINFPIVA
                     NDSSRASISLFINKIVEAYKDGIKNAPKPVAPVAAAKAPEVK"
     gene            15921..16520
                     /gene="tsf"
                     /locus_tag="P5056_00580"
     CDS             15921..16520
                     /gene="tsf"
                     /locus_tag="P5056_00580"
                     /inference="COORDINATES: protein motif:HMM:NF013085.2"
                     /note="EF-Ts; functions during elongation stage of protein
                     translation; forms a dimer; associates with EF-Tu-GDP
                     complex and promotes exchange of GDP to GTP resulting in
                     regeneration of the active form of EF-Tu; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HRY62259.1"
                     /translation="MAITTEQIKKLRDLTGGVSVMQCRKALEDAEGDEAKALLLLSKK
                     GAEVAAKKQERTLGAGIVQAYVHSSATMGALVELNSESDFVAKNEAFKALAYDIAMHI
                     VALKPEYLKESDVPADALDKISKMFAEEVEKSNKPEDIKKKMLEGKIASYVGERTLLK
                     QPFIKNPDQTIEDLIKAHIQKFGEKIEIARFARFSVLDK"
     gene            16524..16910
                     /locus_tag="P5056_00585"
     CDS             16524..16910
                     /locus_tag="P5056_00585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62260.1"
                     /translation="MNYLVSTFLISFFGALYVLVRRVHENRTVGVSGDHSHDAYWPLN
                     VIWSYVRGIVRKVYLIVHNLLAPHIEKAIGLAASKLYRFTSSASHQFLQLSNMVHGKG
                     TLKKNTGTTSLFIRDIADYKKGVNRK"
     gene            16944..17019
                     /locus_tag="P5056_00590"
     tRNA            16944..17019
                     /locus_tag="P5056_00590"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16977..16979,aa:Thr,seq:tgt)
     gene            17124..17891
                     /gene="pheS"
                     /locus_tag="P5056_00595"
     CDS             17124..17891
                     /gene="pheS"
                     /locus_tag="P5056_00595"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HRY62261.1"
                     /translation="MEVLTRDNLLKKGHIHPITQTIVEIVEIFRGLGFVVADGPEIEK
                     EFYNFNALNVPADHPARDMQDTFWLKPLSERRVLRTQTSAVQVRFMEVHKPPLRIIAP
                     GKIFRNEATDATHEAQFHQIEGLLVDKNVSVAHLKGTLEHFLRKLYGESVEIRLRPSY
                     FPFVEPGFEIDMSCTGCIKNEKAGGCCPMCKGTTWIEIMGAGMVHPNVLSAVGLDPKE
                     WKGFAFGLGLDRLVMLKYGVEDIRHLYSGDLRFIDQF"
     gene            17957..19888
                     /locus_tag="P5056_00600"
     CDS             17957..19888
                     /locus_tag="P5056_00600"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF015448.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HRY62262.1"
                     /translation="MRISYNWIKTHFNTEIPAPEAIADALIFHSFEVEGIEKDEKGNT
                     FLEIKILPDRAHDCLCHLGISREISALCGLSKPASFVKELADAGIKHSGPSVNIENKD
                     LCRRYVGRVVKNIKVGETSEHIKELLAGIGQRSINNVVDAANFVMFEMGQPLHAFDLD
                     KIVGNIYVRNAKKGEKMRTLDDKDLELSESILVIADEEGPLAIAGVKGGKRAGVTEQT
                     KNIFLESANFEPYSVRRTSTRLNLRTDASKRFENDYSRVLAMEAIRLFSGLLKETCGP
                     DAVFEDVLDVYPGKTEPRPVVFSAAEISALLGVKIGEDEILKLLDKTFVSAVIKNGKI
                     EAAAPAWRLDLAIVPDFAEEVGRLYGYEKIPNILPEKVSPDIVNKEYPIVNKIKQFLS
                     SAGFSEIYGYSLTDSGEVELDNPLASDKSFMRENLSRQLEEKINLNLRNSLFVEDPVS
                     IFEIGNVFKKEGEQKNICIGIGYKTKKQNKSKAQVMEVFSSLFAELGIKNAPKFSITE
                     RDTLTILEFPLQDILNAVSEVSFDADKIINKEASYKKISQYPRSVRDIALFVPPETDS
                     AAVVKKITNVAGPLLNYGPVLFDVFEKKDADGRPIKKSLAFRLVFQSYEKTLQDEDVN
                     AAMSRITEALKSELSYEIR"
     gene            20143..21219
                     /locus_tag="P5056_00605"
     CDS             20143..21219
                     /locus_tag="P5056_00605"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HRY62263.1"
                     /translation="MEEFADLCEDRLKKFETLLRDPKNVGRRAYVVSSHYDPDSIACV
                     MMTATLLRSFGFIVEICLPGKPDNYRQNIKIVEKYHLERDITLLDEAMIKKINPEDLV
                     VFVDTPSVNDARFPAGTLHPNIVIDHHTRPAEMVEKDEEWFWYASCGACASMVTKLMI
                     MLGVFKDIKDKELKIIATLGVLGILGDAKKLTSRHTTSFDYDMVAFLRRYADQEAIND
                     VSFSEYDESFLDALGISRDRWERRGTVLLFRVDEPHLRGSSEDNILKAAEFLMRIGEV
                     QTLFAWMIQRDKIIIKARNYDKSISLDSELKRMFGQKNGGAKDNSSGAAFVDLSFFSN
                     PPDGIRNQFLSVCEAFMRQKIFGK"
BASE COUNT         6461 a         3989 c         5315 g         5801 t
ORIGIN      
        1 aatgctttgg ttctaagatg aaaactgagg ccagaaaaaa ggcggtagag atgaggaaag
       61 ccggcgccag ttatggtgag ataaaaaggc gtctaaatgt gtccaaaagc acgcttagtt
      121 attggttgcg agaaatgcct ttggcaccaa aaagactttt ggaattaagg cgtaaggggt
      181 ggtcaaaagg ggaggcctcc agagaaaaat acagagaaac gatgagaagg aagaaggaag
      241 ataggtctcg taagatatat gataaatatg cggaacgttt ctcgcgtctg agtgacgatt
      301 cgatattcgt ggcagggctt atgttgtatc tcgctgaggg ggctaaaaag aaagagtcta
      361 ctatcgtgat cgcaaatacc gattcaggcg taataaaatt ttttatgaga tggcttgaga
      421 aatattttgg tatagatcgc agcgaattcc gcatacagct tcatttatat gaagacatgg
      481 atttaaataa ggaaagaaat ttttggaaaa aggaacttgt tattggttcc aatcaaattt
      541 ataaactttc agttagaaaa ttaagaccgg ctagttttac ctacaaggat tcatttcgtc
      601 acggcacctg tagcctatat gcttttggtg ttgatagggt ggtcgagata atgtcagcaa
      661 tgcgcgcttt tgttgacaca gtgaacaaaa ataaataata tatatggatt aaaagcgcgt
      721 gtagctcagc tggtagagca aacgactgat actcgttagg tcccaggttc gactcctggc
      781 acgcgcacaa taaaccgcct tttggcggtt tttcttttaa gccttgtgat gttattatat
      841 tatccgtatt aacaattagt aatttataaa aaaatgaata aaacactttg gataattttg
      901 ggagttgcat tagttgtgtt gggttatgta tggatgacat acaatggctt agtaacagga
      961 agcgcgcttg tagataatca gtggaagcag gttgaggttc agtatcagag gagacttgat
     1021 cttatcccaa atttggttga atcagttaag ggaataatga agcaggagac cgcaactctt
     1081 accttggtaa cagacgccag aacaaaatat atgaacgccg ctactactgg agataaggtt
     1141 actgcggcta acgaattaga gagcgctgtt ggtaagtttt tggtgcttgc cgagaattat
     1201 ccgcagttga ggtctgttga ggccgtcctt agcatgcagg ctgagctcgc cggcacagag
     1261 aacagggtag ctgtcgagag aatgagatat aacgacaacg tcacaagttt caatcttatg
     1321 acaaagagat ttccttcaaa catgattgct tctatgttcg gctttgagca ggctgtatat
     1381 ttcaacgccg agaagggggc agagaacgtt cctaaggtac agctctaata accttaatga
     1441 aaatactgaa aatatttggg gcgataataa tatttgcctc gctaacttca gttgtaagcg
     1501 catattacag ccttgggagg ccggctggtt ttgtctacga cggagcaggt gttttgagtg
     1561 ccgagcaaaa aactgctctc gaatctaagc tttctctgtt tgagaaagaa acaagcaatg
     1621 agattgcgat tgcagtcatt ccaaatcttg gtggagatta cattgaaaat tttgctatta
     1681 aattgtttga ggagtggaag ccgggaaagg ccaataaaga caacggtgtc ttgattttgg
     1741 ttgcgattga ggacagagaa atgaggatag aggtcggata cggcctcgag ggcgcactta
     1801 cagatagtca ggccaattgg ataatcagaa acgtgatgac cccggctttc cgcgacggca
     1861 actattctgt tggcatagat ggagccgtgg ataagataat cggcgcgaca aaaggggagt
     1921 atgttccgga cgataatggt cccgcggata gcaccgcttt cgaaaatata tttccgctta
     1981 tattcttcgt tttgtttttg gttctcgatg tcttgataat ggtttttggc cgatcaaaat
     2041 catactggga gggtggcgtc ttcggcttag ttgttggcgc tttaattggg attatttttg
     2101 tatcttattt tgccggattt ttgttagcct tcatattcgg ggtgcttggc ctcgcttttg
     2161 attatttcgt ttctcgcgtc gtcgacccag aaaagataag gagacacata ggtggaggtg
     2221 gatttggtgg tcatggaggt tttggcggag gcggtggatt tggaggattc ggtggcggca
     2281 gttccggcgg agggggctcg agcgggaagt ggtaataatt ttataaaacc ggccttgtgg
     2341 ccggtttttg cttgatttga ctatatttgg ctaatatgtt agtctgcttt ttggtaagtt
     2401 cattaaatca ggagaaaaag aaagggagga agccgcatga aggacaacag agaacaaaac
     2461 atcctcgaca ggattcgtga cgaaaagttc acggacgacg agatctttcg cgaagtgtcc
     2521 tcgggtctct actcggagga ccagaatgcc gtcatccacg ttcttcaaaa ctaccgtccg
     2581 tgtctcttga agcagcttct ctgcttcgtc gaagaatagg aggggcgatg ccgaaacttg
     2641 agcagaacga gatgatcttt caggggatcg gtatccccat cgagacgatg atcacagacc
     2701 cccgcgttcc cgacgatgtt cttcggcttg aattcgagcc tcttcagcac catggccacg
     2761 tcctctttct gatgaaggcg catcggccta agctcgccga cgaactgttc cacaccttcg
     2821 atttctccaa gaattaaagc agacgaaacc caggcacttc gcctgggttt aacttttttg
     2881 agaataggtg taagatagct gaaacgttcc ttgcaatgtt tctaaaagga ggaggtgata
     2941 ctttgtgcgc cccaatcttc gttatcgggt cctggatctt tccgtttccg attatgaatt
     3001 gtttagggag attacagagt atctccacct tgaggatgat tacgatcttt ttgtatttat
     3061 ggagaaatat cgccccaaac ttttagaatc tatctttaag gcccactgac aacaggtggg
     3121 tcttgctttt tttacattaa tattgtatta tttgacttcg ttaaattcac aagtaaatga
     3181 acgaagacat ccgaaacatc gcaataatcg cccacgttga ccacggcaaa actacgctcg
     3241 tggatgcttt gttgcgccaa gcaaaaacaa acatcaagga cgaggtcttg aacacagagt
     3301 gcataatgga ttcgaacgag attgagaagg aaagaggcat cactattttt gcgaagaatg
     3361 cttctgttag atatggaaat acaaaaataa acattcttga tactccgggt cacgctgatt
     3421 tcggcggaga ggtagagcgc gtgcttacta tggctgatgc ggcgcttctt ctcatcgatg
     3481 caaaggaagg cccgatgccg cagacgagat tcgtgctcaa gaaggcgctt cagcttggtc
     3541 tcaaaataat tgttgtaata aataaaatgg ataagccgga agctaggccg gcgtatgttc
     3601 ttgatgaggc gttgaatctt tttatagagc ttggagcgaa tgatgcccag cttgattttc
     3661 ctgtcattta ttctatttca aaacaaggga aggctggcat gaagccggat gtttcagaaa
     3721 tgaaagacat tactccgata tttgagacta ttttgaaatt tgttccgcct gccgagagag
     3781 atgtggatgc aaatctgcag tttttgacag ttaatatttc ccgagatgat ttcaagggca
     3841 gaattgcgac cggaaagatt tacaaaggaa agataaaaag cggggagact gtttcttgca
     3901 taggacgtga cggtaaggcc gttaatttta aggtgaccgc gcttatggtt tccgatggac
     3961 tcggtagggt tgaggtgtgc gaggcgggtg cgggcgacat cgttcatatt gccggttatc
     4021 ccgacatagg catcggtgat actatttctt ctaaagataa tcccgtggcg ctcacaaggc
     4081 ttaagataga cgagccaacg atccaggttg tctttatggt taacaattct ccgtttgcgg
     4141 gcagagaggg aacttatgtc acctcaagac agcttcgcga gaggctttat aaagaaatgg
     4201 agactgacgt agcccttcgc gtagaagata caaattttcc ggagaaattc ctcgtttcag
     4261 gaagagggga gcttcatatc ggaatcctca ttgagaggat gaggagagag ggatacgaat
     4321 ttcaggttgg aagaccgaag gttatctaca aagacattga tggcgttaag aatgagcctt
     4381 atgaagaggt ctctatcgag gtgcctgata atttcgcagg cacgataatt gaaaaaatgg
     4441 gaacaagagg tgctgagatg aaagatatgc gattgcacga ggggataaca caccttacat
     4501 ttataatccc tacgagtaga ttcctcggct tcagatcgaa gtttatgacg gacacaaaag
     4561 gtctcggtat tttgaattct ttattttacg gctatttgcc ggcggtggag gcaagacctc
     4621 agccacacgg ttccgttgtt gcttatgaag atggtcaaac cacagctttt gctctcgaga
     4681 atgcacaact tcgcggcaca atgttcgtcc caccgggagt gcaggtatac agaggccagg
     4741 ttgtcgggag gtgctccaga gatgaagatc ttgaaattaa tgtttgtaaa aagaaagaac
     4801 tttcaaacat gagaagcact tctagcgatg gtatggtgat gctcgacccg catgttgaaa
     4861 tgggagtaga ggatgcactt gaatatatca gcgacgatga gcttgttgag gttacgccaa
     4921 aatcagtcag aatgagaagc ttgaaggtgt ttaagacgaa gggacattag agttagttat
     4981 aaagttaaat gttataaagt tgtaaagtta gcccacttgg aagctttgcc tccaagtggg
     5041 ttttgtcttt gcgagcctcg cgaagcaatc cagagggggt agtgtcattc ccgtgcagac
     5101 gggaatccag acttttagac gttccatgtc caaatcgagg acgggggctt ataaataagc
     5161 gctttttagc ttatcagcaa aaatgtttga caagacaata gattaagtgt ataatttaaa
     5221 aaaataacta aaggttcttt aaaaaagggg gtagtgaaat atgtctgtga ttagttggct
     5281 gctactacca ttcaattttt tgttttcagt gctgtggtgg atctgcttca ttgtccttaa
     5341 tggcattgtc cttgcggcta tactttttat catctgtttg ccattcattc ttatgtatgc
     5401 cgcatctaaa gtgccggata ggatgacgca cgcagaaaag cggtatcgcg actacatgct
     5461 ttatgagaag tatcacgagc caccgccgga ttatgaagaa ccggaagata aaacgaactg
     5521 aactttcgcg ccaagcgcgc aggaaaggaa acggtaaaaa tggatttctg gatggtccac
     5581 ggaattctct tcctcatctg ctgcgcactc ttccctcgtc tcaccatgtt cttctcggga
     5641 actgttctcg gcttcggctt tcttggttgg tttggctgga ttttctttcc tcgtattacc
     5701 atcgccgtcc tggccaccaa ctcgtactgg gataccaacc ccgttctcgt cgtgattgca
     5761 tggatgattg cctttactag cgagagcaaa gaaaagaagg ccctgcacag atagttttat
     5821 tagcagaacg cagttcgccg ccgtgacttc ggtcccggcg gcttttttat tgcttaaaat
     5881 gtcattgcga gctttgcgaa gcaatccaga aattaattct gttcggtgtt caagggatct
     5941 ctcgacaagc tcgagatgac agttatcgaa cgaattcgta attcgtaatt tttaattcct
     6001 aattcattct cacttctccc aacgactccg ttatcaaccc ttttatttcc attacagaaa
     6061 gtagggtatt tgattcactt attggcaatt ttaattcgcg gatgaggtcg tcgcggggga
     6121 gcggctcgat agataaaatt tcaagaactt ttcgttcgtt ttccgatagc gcggggtctt
     6181 tcaggtccag cgctttttgt tttgtttcta cgtcgatgcc gagcgctttc aatatttctt
     6241 cgccggatga gacgggagtc gcgccgagat ttattagctt gtttgagccg gccgcgttcg
     6301 gtgaaaatat tgagccgggg acgacgagaa gatctcggtt gtattcggtc gcaagtcgtg
     6361 cggttatgag cgtgccggat ttgtctccag cttctataac aagtatcgct tttgcgagtc
     6421 cggccattat cctgtttctc tgcgggaaag tgtaacgggt cgctctttgc attgggtcaa
     6481 attcagatag tagcgcgccg cccttgctga ctatttcatc ggcaattttt ctattgagcg
     6541 aaggataaag aacttttcta tcaagtcctg agcccggtac accgacagtt tttaaacccg
     6601 ctttcatggc ggctttgtgt gcgatggcat ctatcccgtg cgcgagtccg gagactatca
     6661 caatattata tcccgcgagc tcgcttataa gtttttcaca agcgtctttg ccatagtttg
     6721 aaaatttcct tgagccgaca acggtgaggt agatatattc attttcgtca gggagttcgc
     6781 cttcaacata aagttgtttg ggcggttgcg gaatctctaa tagggcctta gggaactcgt
     6841 tttggtttaa ttttctgatt gagtctttca tcttgcgtaa tatagcatag gatatagcgc
     6901 aaagcgaaaa gctaaaaaca aaaaataata agataaatat atggtaatgt ttttaatttt
     6961 gagttgttgt tttgtatttt tagattttca ttttacgctt gctctcaaag tcagacggaa
     7021 gattttgtgg agacgtggtt gagaactgct gaaattatgt tatgattccc atgtacccca
     7081 gtgaaatagg agtctgctgg gaatatagag attaaataat ttttaagaaa caaattcgaa
     7141 cttcgcggtt taataatgtt aatttcacgg ggtaaatgtt agatatcaaa tttataaaag
     7201 aaaatccgga cctcataaaa gaggcggcca ggaaaaagca cagcaatttt aaagttgagg
     7261 agctcattga ggccgacggc gacagagttg ccctgatcaa ggaggtggac gaaatacgcg
     7321 ccaagcagaa cgctatgaat ccaaagatat ccagcacgtc cgatatgaaa gaaaggaatg
     7381 acctcatttt ccagatgaag gaaataaaag atgaacttca gaaaaaagag gcaagacttg
     7441 aagaagttat gaaaaagtgg aggtcactca tggtacaggt gcccaatgtt cctgatatgt
     7501 ctgtgccgga gggtgcgagc gatgccgaca acaaagaaat aaaaacttgg ggaaatattg
     7561 cgaagtttga tttcacacca aagagccagc acgaaattct tttggacctc gatatggttg
     7621 attttgacag aggaataaaa gtttccggct tcagaggata tttcctgaag aatgacggat
     7681 tcttgttatc gatggccatc tggcaattct gcatagacag tttggttaaa aaaggatatt
     7741 caccgcttct tgctccgagc ttggttaaga aagaaccttt ttatggaaca ggttatctac
     7801 cacaggggga ggaggactta tacaagactc aagatgccga ttatttggcc ggtaccgctg
     7861 aggtgggcac aatgggtttc tatatggatg agatagttcc gaaagaaatt ctgcctaaga
     7921 aatttgtttc tttctctcca tgtttcagac gggaggccgg aagccacggc aaggatacaa
     7981 aaggacttat tagactgcac gagttctaca aggttgagca ggttatactt tgcgaggcga
     8041 gccacgagca gagtgtaaaa ctccacgagg aacttcgtga aaacgctgaa gagataatgc
     8101 agaagctcgg tattccatac agagtggtta taaattgcgg aggcgatctc ggcctgggtc
     8161 aggtcaaaaa atacgatata gaagcatggg tgcctagtga ggggaagtat cgggagacac
     8221 actctatttc ttattttcac gatttccaga cgagaagact gaataccaaa tacaaggatg
     8281 aagacggaaa gttgaagttt gcgcattcat tgaacgggac agctcttgct acgcctagag
     8341 cgcttgtcgc gatagtggaa aatttccaaa acgccgacgg ttccatcaca atcccggagg
     8401 ttttgaggcc atatatggga ggaagaacaa agatagaaaa accacaggtt taaataaatt
     8461 cgtttttgaa ctacaattaa gtagtgagtt tgcaacggga cgccttaaaa tcttctgcca
     8521 aattgaggag aaagaaaaag ttcaatactt tttccatttt tctttgcact tttttgttta
     8581 taacgttatc tctcgcgggc ttgtctcaaa tagacagatt tctgataaaa aaaattaaaa
     8641 tatccggaga aaggactatc tcgcgcgcag ttctggccaa tcagataaaa ccaagactag
     8701 agggaaggta ttttggtctt ttcccaaaaa ataatgcttt tatatatggg aggcggggga
     8761 tgatagattt tcttaagact aatattttaa agatcgaaga agcggaagtg tctcttgatg
     8821 acctcaatac tctctcaata aatatcacag aaaggcaagc tcggcttttg tggtgcaaaa
     8881 atgacccaaa gacgtgttat tacgcagacg gaaacgggct catctttgat agggcgccgc
     8941 atttttctga cggagtattt ttgaggtttg agggcggtct tccctccgag gaaagtgatt
     9001 catattatgt tggaaaaaat atttttccgg ctgaatatat aaaaatgctt ataactttca
     9061 gacagagggc ggaggaattg ttgaaaaaaa atttttcaga taattggaag gcttcatatt
     9121 attttcttgg tgaggggagt gatatagatc tattttttga gaatggaata gggggcaagt
     9181 ggaaaattat gatgtcccca agaacagaag aaggatattc tttcaagggc aatgactcga
     9241 gcggcctact taagccggaa actgaggcga cttgggccgg ttttggagga gatttggaga
     9301 gcgcggaggc taacctgaaa gttattttgt cttcagaggc gtttagggga agcgccgata
     9361 acaagaccga accgcttaaa aatttagagt atatagacct gcgtttcggc aaaaaaatct
     9421 tctataagtt taattagttt atagttaata gtttgtagtt tttattttgt tgattaacta
     9481 ccaactacta tctacaaact ttttgtttag ggtgccaatt attgtctacc cataaaagcc
     9541 ccaaataaag gctattaaag atttgcaatt ctatcttcta tatgtataat atatgtccat
     9601 gttaacttta accgcaaaaa agagagatac gttgggtaaa aaggtaaaat cccttagata
     9661 tgaggacctt attcccgtcg ttgtatacgg cccgggagag ccgactgcac attattcggt
     9721 aaaattaaac gagtttatta aagtttggaa aaaggccgga gagacagtcg ttgtagccat
     9781 agaaaccgat tctgacggcg taaagaatac tcttatacag gacgttgttg taaatccaat
     9841 tacagggact cctatccacg ccgactttta tgccgttaag aagggtatga ctgtttccgt
     9901 aaacatcccg ctcagattcg aaggagatgc tcctgctatc aaggcttttg gaggaatttt
     9961 ggtaagagtt atgcacaacg ttgaggttga ggcagagccc gataaacttc ctcaggaaat
    10021 tgtcgttaat gtttcctctc ttgagaccct ctcggacaga gttactgtta aggatttgaa
    10081 actgggagaa ggggtaagag ctgttacatc accggatgag gttatcgctc ttgtttcggc
    10141 ggttgttgaa aaagaagaga aagttgaaga gcctgccgat ctttccaaga ttgaagttga
    10201 aaagaagggt aagaagcctg aagaaggaga agagggagca gagcctgcaa agaaataaga
    10261 taagagtcag gaagaaagaa ttaggaatta agaataattt gaaaaccggg ggccctcata
    10321 tgagggcctt tagttttcaa aaagataatt ttagatatac ttaatagtat gaatttatca
    10381 acgtctctca aaacgaaaaa gacgattttt ttgggactat tgttcttgac ctttttccct
    10441 gttgtttctt tcgcagaaga tacaagtatc tcttgtgata ctgttaatga aaatgagcgg
    10501 cccgaggtgc tcgataagaa atttttgaag tgccaagaag aaattttggc gcatcagcaa
    10561 cttatacagg aaaaagaaaa agaatccgcg agcttgcaaa gagatcttaa acttataacc
    10621 gcgaaggtgg aaaaaactaa gtcggagatt aaggcgagaa atattgccat cgttaacatt
    10681 gaaagggaga tagacaagaa aaaccaaaat atagaggaga tgaatgtaaa aatggacgat
    10741 ataaggacat ctacggccga gcttattcga gaaacagaag atttagagtc tattactttg
    10801 cctgaagtta ttttggacaa tagaaatatt tcggaatttt tcaacgacct tgcttctttt
    10861 gagtcggtag aggcttcttt gaaggagacc ctaaacaaat acaaagacat acagaaaagt
    10921 gaagaggagg ccaaaaagga tttggctggt aaaaaagaaa aggagagggg acttaaatct
    10981 cttcaggaaa tagagaaaag aaagactgag ctcgcagagg ctgagaaggc gagaattttg
    11041 aaggagacta agggggaaga aataaaatat aaagcgatgc ttgcggaaaa gcaggccctt
    11101 gtgaataaaa taaagaacat gatgttgaag ctaacgggtg gaggtgaact taagtttgcc
    11161 gaggctcttc gtttggtccg tgttgccgag caggcgatag gggttagagc agcttttgtt
    11221 ctttctattt taacccaaga gtctgggatg gatggagcga taggagctaa tttgggaaag
    11281 tgtttctaca ataccccgtg gaacaataaa tccggaactg ttatgagcga cacacagaaa
    11341 cccgcatatt tggctttgct ttctgaaata ggaaagaatc ctaatactac accggtatct
    11401 tgtccgataa gcaaagacgg tcaatacgga ggagctatgg gtccgtctca gtttatgcca
    11461 aagacttggt gggacggcgc ggcgggaacc ggatataaaa agagggtgga aaaggtcacc
    11521 ggtaacgtgc cggcatctcc attcaataat ctggatgcat ttaccgctac cgcactgtat
    11581 ctcagcgatg cgttggaggg ttgtgagaag acctatacaa gcaaatataa tcaggaaaaa
    11641 tgtgcggccg ccaagtatta cgccggagct aactggaaga agcacctaaa cggctatgga
    11701 gccaaggtgg caaatagggc cgtagaatta cagaaggata ttgacgtcct tgactcgcag
    11761 taaactattt gataatctat cccttatgaa agataaaatt catccaaaat ataacgataa
    11821 agcaaaggtt atttgctcat gtggaaacca gtttgaggtt ggttctgtgc tacctgagat
    11881 cagagttgaa atctgcggta agtgccaccc attctttaca ggtaacgaga ggattgtaga
    11941 cacagccgga agagttgaga aattcaaggc aagagccgcg aagaagaccg gcacaaagaa
    12001 ggagaaatag tttaatctct tgaaacgccc tttgattttt tagagggcgt tttgatttgc
    12061 gcaattatta atttacaata agattacgac tacgcttaga aattgaaaat tttttatgga
    12121 cgaaaaacta ttaaaactta aagacaatcc aaagaccaca tatcttttca aaggatatga
    12181 gcgtattttg gctgagatag aagaaaatca aaaaatagtt gagagtgacc ccgagcttaa
    12241 agaaatagca gaggctgaca ttaagaaact cgaagaaagc caatcatccc ttctttctca
    12301 gatggaggag atagcaaaag aagaagaggc ggcggctctt gatccgaaag agatgattct
    12361 ggaggtgcga gccggagccg gaggagatga agcggccata ttcgcgagag agcttgccga
    12421 ggcatatatg agatatgccg agctgaaggg ctgggcatgg aaaccgatag atgaatctga
    12481 gtcagaactt ggcggataca aagaagcttc ttttgaaata agggggaagg gggcatactc
    12541 tcatttgaaa tacgaaatgg gagtgcatag agttcaaaga gtccccgcca cagagaagca
    12601 gggcagggtt catacttcca ccgcttctgt cgcggtattg ccgattgttg aaagcaagaa
    12661 gattgcaatt ccggaaagcg atatagagat ggaattttct cgtgccggag gaaagggtgg
    12721 ccagaacgta aacaaggtag agacggccgt tcgtcttgtg cacaagccta ccggtatcat
    12781 ggttcgttgc accagcgaga gaagtcagct caagaataga gaaacggcca tgagcattct
    12841 tgcggcgaag gtggctgagc tgaagagggt agaggaggag aaaaaattat ctagcgaaag
    12901 aaagagtcag atcggcacag gcgacagatc tgaaaagata agaacctaca atttcccgca
    12961 aaacagaatt acggaccacc gaataaaaga atcgtggcac aatattgaga aagtgatggc
    13021 cggcgactta gaccccatca ttgatgctct tatcgctttc gataaagggg agagaaccgt
    13081 cggatcttca gacgaagaat aatcttaatt tttaaaaacc accagataca atatctggtg
    13141 gtttttccac atggcccatc atagattttg cttagcaaag agtgttataa tagttttgta
    13201 taagttaatc taattattat gtctcataaa aaacacctac tgcttttgac gcttttgtcc
    13261 atagttttcg cgccgggact atatttttct cacaacgatc cgatacctgc tatcgcgcaa
    13321 agcggaagca acaagctttc cggctgggca tggagctcga atgtgggctg gattagtttt
    13381 aactcagacg attgcaccac ttccgccaac ttcataaata atgcctgtgg aggtaacggt
    13441 ataaagaaat tggtagattc aagcggaacg ccaataactt acggagtgac cttagacagc
    13501 tcaaataatc tagtcggtta tgcatggaat ccggatctcg gatatatcta ttttggagga
    13561 gattctgata acgcgggcgg aatagaaagc gcccctgagg ctccaaagac gtgggctcgc
    13621 ttaagcggca acgcagtaaa cagccaaatc acgggttggg ctagagcgtg ttcggttttt
    13681 gcaagcgggt gtaacggcgc gcttaaaagc aatatacaaa gaggtgattg ggatggttgg
    13741 ataaagatgt ccgatgacgc cggcacaatc aagtatggcg cttacataaa agatatcgcc
    13801 accaatggcc agaaaatatt cggaggtttt gcttggggtg gagggagcaa tcccgcaatg
    13861 tctagctcgg cacaatttcc gggttggata aattttgact ataagacaaa tccggacggc
    13921 acgcctgtta cacccggttg tgtaggtcaa agttgccagg caggatgcac cgagaactgc
    13981 ggtggcgtag ttgtgcctcc tagcgtgggt ccggagacgt tttttgttcc taggtgttta
    14041 tcggaattgg cggagggggt aacagtagtg gagcccaaca agactctgac attctctgtt
    14101 aatccagagg gtcctggtaa taatgacaat tatacaataa ggtggtttga tgatggagtt
    14161 gaaaaaccgg gccaaacaac gagaagctat accttaacta caagcgcttc ggttccaagc
    14221 cataatataa ctgtcagtgt ggtcccaaac ccagctgggg gcgttgctgt aaggaccctt
    14281 tcatgcacgc caggaggatt gaccgttgcc gcttctccta ctgcgtcatt aatcgccttc
    14341 tctgacctca atctaatgaa tatgagcgtt atcttgccag agggcgcgat tccggtaacg
    14401 gcaaaaaata tttctggagt tttgcctaaa ataacaataa ttgatgccac gggttctcca
    14461 tgcagcgcgc aaattacttt atctgtggaa aaaataacga agaatggcgt gccaatacca
    14521 gaagttcatt ggaagaaatc gactgcgccc aaccagtctc cagtattttc tccaaaaaac
    14581 ctaatatctg ttggcagctc atctaatttg accttgaaca taataagaca gccggctgga
    14641 acgacaagac tttctgacgg aacatattcg gtcaacataa aagcttcgcc tgcaggtggc
    14701 gcttgtggag gggatcgtta tcgtgatgcc gcccttaaaa tatcctctac cggtggcggc
    14761 ggaacctaca tagaggaata aaataggcca tataatagaa aagagcccct tccggggctc
    14821 ttttgacgtg tggggagcct tcttggttgc tatttagccc catttttgct agtataacca
    14881 gacgattgtt tcgctaagta ttcaaaccgg cggaacgaaa ataaacagta tttttaaaga
    14941 cggaaacgtc ttaattcttt attcataatt cattttaaat atggcagaaa tagaacaaaa
    15001 attagatttt tcaggattgg aagataagag tctcgccgct ttagttgagg agcttcacaa
    15061 agctggggcc cacttcggat attccagatc aaggagacac ccttccgtta agaactatgt
    15121 cttcggttca aagaacagaa tggacatcat tgatcttgag cctatggcca agtcgttgaa
    15181 agccgctctt gaatttgtag gaaggcttgg agccgagggc aaacagatac tttttgtcgg
    15241 aaacaaaaaa gaggctcagg atgctgttaa ggccggagct gaaaaattga atatgccttg
    15301 ggtagcattg agatggatgg gaggcacgct cacaaacgcg ccggagatca gaaagagggt
    15361 tgccagaatg gaggacctta tcgccaagaa agcggttgga ggtcttgata tgtatacaaa
    15421 gaaagagaga cttctcttgg acagagaggt tgcaaagctt cagaaacact tcggcggtgt
    15481 tgtaagtatg aagagagctc ccgccgctgt tgtcgtggta gatagcagaa acgaagaaac
    15541 agctatagct gaagcaaaga tggcaaatat cccggtaatc tcattgtcgg gaacagacaa
    15601 caacatagcg ggaattaatt tccctatagt tgccaacgat tcttcaagag caagcatttc
    15661 tctgttcata aataagatcg ttgaggcata taaggacggc atcaaaaatg ctccaaagcc
    15721 tgtagcacct gttgcagcag ctaaggcccc cgaggtaaaa tagaggaagt tgattacgaa
    15781 ttcaccctgt taagtaaaat tttcttaaaa aatttaaaat ttgtttaaga taaagaacga
    15841 gacgggtagg tcaggatatt ttagtaaaaa taacattatc aagtaaaaac aattaaacaa
    15901 atttttactt aacagggtaa atggctatta caaccgaaca aattaagaag ttaagggatc
    15961 ttactggagg ggtttctgtt atgcagtgca gaaaggcgct cgaagacgcc gaaggagatg
    16021 aggccaaggc cttattgctt ctttcaaaga aaggcgccga ggttgccgca aaaaaacagg
    16081 agagaactct aggagccgga attgtgcagg cttatgttca ctctagcgcc actatgggtg
    16141 cgcttgtaga attgaattcc gaatctgact tcgtcgcaaa gaatgaagct ttcaaagctc
    16201 ttgcttacga tattgctatg cacatagttg cccttaagcc tgaatatttg aaagagagcg
    16261 atgttcccgc agatgctttg gataaaataa gtaagatgtt cgcagaggag gtagaaaagt
    16321 cgaataagcc tgaagacata aagaagaaga tgctcgaagg aaagatcgct tcttacgtcg
    16381 gagagagaac tcttttgaaa cagccgttca ttaagaaccc tgatcaaaca atcgaggatc
    16441 ttataaaggc ccatattcag aaatttggag agaagataga gatagctaga ttcgccagat
    16501 tttctgttct ggataaataa aggatgaatt atcttgtttc tacattttta atttcttttt
    16561 tcggcgccct ctacgttctt gttaggaggg tgcacgaaaa cagaactgtc ggggtctcgg
    16621 gcgatcattc acatgatgca tactggcctc ttaatgttat ttggagttac gttcgaggca
    16681 ttgtaagaaa agtttatttg atagtgcaca accttctcgc ccctcatatt gagaaagcga
    16741 taggtcttgc ggcttctaag ctttacagat ttacatcatc cgcctcgcat cagttccttc
    16801 agttatcaaa tatggttcac ggaaaaggca ccctaaagaa gaacacaggg acaacgtcac
    16861 tttttataag agatattgca gattataaaa agggggttaa cagaaaataa gttatctgct
    16921 attatagttt ttgttgtaaa gttgccaatg tagctcagtt ggtagagcaa ttgttttgta
    16981 aacaataggt cgtcggttcg aacccgacca ttggctccat ataaaaagcc cctttaaaag
    17041 gggacttttt atttatatgg agccaatggt tggtcccccc ccaacttatt ggaaaaaacg
    17101 ccattttccg atatactatc cgaatggaag ttttgaccag ggataatcta ttaaagaaag
    17161 ggcatataca ccccataacg caaacaatcg tcgagattgt cgagattttt aggggccttg
    17221 gattcgttgt cgccgatggt ccggaaatag aaaaagaatt ctataatttt aacgctttga
    17281 atgttcccgc agaccatccc gccagagata tgcaggacac cttttggctt aagccactct
    17341 cagagaggcg tgttttgcgc acccagacaa gcgctgtgca ggtaaggttc atggaggtgc
    17401 ataagccgcc gcttcgcatt atcgctccag gtaagatatt caggaatgag gcgacagacg
    17461 ctacgcacga agcccaattt catcagatag aaggtctttt ggttgataag aatgtgagtg
    17521 ttgctcatct caagggcaca ctggagcact ttttaagaaa actttacggt gagtcagtag
    17581 aaataagatt aaggccgagt tattttccgt tcgttgagcc cggatttgaa atagatatgt
    17641 cttgcacagg atgtataaag aatgaaaagg ccggaggctg ttgccctatg tgcaagggca
    17701 ccacttggat agaaataatg ggagcaggaa tggtccaccc gaatgttttg agcgcggtcg
    17761 gcctggatcc gaaggagtgg aaaggttttg cttttggatt gggattagat cgtttggtta
    17821 tgctcaaata tggagtggaa gatattagac atctttattc aggagacctt aggttcatag
    17881 atcagtttta ggttagatag tagcagatag atagtagttg gtagtttcta aaaactacaa
    17941 actataaact aatcacatga gaatttcata caattggatt aaaacacatt tcaatacaga
    18001 gattcccgcg cctgaggcga ttgccgatgc gcttattttt cattcgtttg aagtcgaggg
    18061 aatagaaaaa gatgaaaagg ggaatacttt tttggaaata aaaattttgc ccgacagagc
    18121 acatgattgt ttgtgccatt tgggaatttc acgagagata tcggcattgt gcggtctcag
    18181 taagccggcc tcgtttgtaa aagagcttgc agacgccgga attaaacaca gcggcccttc
    18241 cgtgaatata gagaacaaag atctgtgcag gagatacgtc ggacgcgtag ttaaaaatat
    18301 taaagtcgga gaaacttcgg agcatataaa ggaactcctg gccggtatag gacaaagatc
    18361 aatcaataac gttgttgatg ccgccaattt tgtaatgttc gaaatgggcc agcctcttca
    18421 cgcttttgac ctggataaga tcgtcgggaa tatttatgta agaaatgcca agaagggaga
    18481 gaagatgagg accctggatg acaaggactt agaactttca gaaagcattc ttgttatcgc
    18541 cgacgaagag gggccgctcg caatagcggg ggtaaaagga gggaaaaggg ccggggttac
    18601 agaacaaaca aaaaatatat tcttggaatc ggccaatttt gagccatata gtgttagaag
    18661 gacctctaca aggctaaatt tgcgcacaga cgcgtctaaa cggtttgaga acgattattc
    18721 cagggttttg gccatggagg ccatacggct gttttcgggc ctcttgaagg aaacctgcgg
    18781 gccggatgcc gtttttgagg atgttttaga cgtttacccc ggtaagacag aaccacgacc
    18841 cgtcgtcttt tcagcggcag aaatatctgc actgctcggc gtaaagatag gtgaagatga
    18901 gattttgaaa cttttagaca agacatttgt ttcggcggta ataaagaacg gaaaaataga
    18961 ggcggccgct cccgcctgga gactggatct cgccatcgtg cccgattttg ccgaagaggt
    19021 tggtaggctt tatggctacg aaaaaatacc aaacattctt cccgaaaaag tttctccgga
    19081 tattgtaaat aaggaatatc cgattgtgaa taaaattaaa caatttttgt cttccgccgg
    19141 tttttcggaa atttacggat attctctcac ggatagtgga gaggttgaat tagataaccc
    19201 actggcttcc gacaaatctt ttatgagaga aaacctctca agacaattgg aagaaaaaat
    19261 aaatcttaat ttgagaaaca gcttgtttgt agaggaccct gtatcaatct ttgagatagg
    19321 taacgtattt aaaaaagaag gggagcaaaa gaatatttgt atcggtatag gttacaaaac
    19381 aaagaagcag aacaagagca aggcacaggt gatggaggtc ttttcgtctc tttttgcaga
    19441 gctcggcata aaaaatgctc caaaattttc tattacagaa agggatactt tgacgatatt
    19501 agagtttcct ttacaagata tattaaacgc agtttctgag gtgtcgtttg atgctgacaa
    19561 aatcataaat aaagaagctt cttataaaaa gatttctcaa tacccgagat ctgttagaga
    19621 cattgcgctg ttcgttccgc ccgagaccga ttctgccgct gttgtaaaaa agattacgaa
    19681 cgttgccggg ccgcttctta attatggacc ggtattgttt gatgtttttg aaaagaaaga
    19741 cgccgacggc agaccgataa agaagtctct cgcattcagg ctcgtttttc aatcttatga
    19801 aaaaactctt caggacgaag atgtaaatgc cgctatgtct aggataactg aggcgcttaa
    19861 gtctgaatta agctacgaaa taagatagat ggcctgcccc gtaggaagtt tctagcagaa
    19921 ctataattaa taaataagat aagccgtagg cggtttccga gcctcagaat attgtctttg
    19981 gtggaacaaa gtcatatgaa ctttcctact ggggcttaaa acaaaactta aaataaaaat
    20041 ggtcgttgac gaggcggcca ttttttggta ttattttgca aaatgttcat tgtaatagtt
    20101 gtgaaataaa agctatttaa cattgtcgga aggagaggga atatggaaga atttgcggat
    20161 ctttgcgaag ataggttgaa aaaatttgaa accctgttgc gcgacccgaa gaatgtcggt
    20221 cgccgcgctt atgttgtaag ctcgcattac gaccctgatt ctatcgcgtg cgttatgatg
    20281 accgccacat tgctcagatc gttcggtttt atcgtggaga tctgtcttcc cggtaagccg
    20341 gataactatc gtcagaatat caaaatcgtg gaaaagtatc atctcgaaag agatattacg
    20401 cttctcgacg aagcaatgat caaaaagata aaccctgagg atcttgttgt gtttgtggat
    20461 accccgtctg tgaatgacgc aaggttcccg gccggcacgc ttcacccgaa tatcgtgatt
    20521 gaccatcaca ctcgccccgc cgaaatggta gaaaaggacg aagagtggtt ttggtacgcc
    20581 tcttgtgggg cttgtgcctc catggtgacc aagctcatga ttatgctcgg ggtctttaag
    20641 gatataaaag acaaagagct taaaatcatc gcgacacttg gggtgctcgg cattcttggt
    20701 gatgcgaaaa agcttacctc aagacatacc acatctttcg attacgacat ggtcgcgttt
    20761 cttcgaagat atgccgacca agaagcaatc aatgatgttt ctttctcaga atatgacgaa
    20821 tcgtttctgg atgccctcgg catttccagg gacaggtggg agagaagggg aacggtctta
    20881 ttgttccgtg ttgatgagcc gcacttgcgc ggttcgagcg aggacaatat tctgaaagcg
    20941 gccgaatttc tgatgagaat cggtgaggtc cagacacttt ttgcatggat gatccagaga
    21001 gataaaataa tcatcaaggc ccgtaattac gacaagagta tctcgcttga ttccgagctc
    21061 aagagaatgt tcgggcaaaa gaacggcgga gcgaaggata attcttcggg cgccgccttc
    21121 gttgacctgt cctttttctc aaatcctccg gacggcatta gaaatcaatt tctttccgtt
    21181 tgtgaggcat ttatgaggca aaaaatcttt ggtaaatagc gttaaaaata atcacaaaat
    21241 aaggctcaac ttgcagaaat gagcattttg ttgtgatgca taccccgtaa ggcaattcgt
    21301 tgcttttcgg ggttttttta ttgtcctcaa tatgataaaa tttacactag aaaatctaga
    21361 tttgagcatc acttgaaagg gcttgtcgag tccacaagaa atagaaaacc atttgagcta
    21421 atctattttg aggcttgctt agatcaagaa gacgccactc ggcgtgaaaa tacttaaaga
    21481 caatatacgg caaaaggttt attaaaagta ggctcaaatc ttacttaata gggtaaatgg
    21541 ccaaagataa agacaaaaaa ggcagt
//
LOCUS       DAOTEX010000005        20525 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3118
            NODE_1718_length_20525_cov_4.019883, whole genome shotgun sequence.
ACCESSION   DAOTEX010000005 DAOTEX010000000
VERSION     DAOTEX010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868142
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 20525)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20525)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 883
            CDSs (total)                      :: 833
            Genes (coding)                    :: 829
            CDSs (with protein)               :: 829
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20525
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1718_length_20525_cov_4.019883"
                     /isolate="AMR_MDS_3118"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1176
                     /gene="murF"
                     /locus_tag="P5056_00610"
     CDS             <1..1176
                     /gene="murF"
                     /locus_tag="P5056_00610"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HRY62264.1"
                     /translation="AIYTIMSGFFDTRKSEKSFNSEIGLPLTILGLKNAWTDPLAWLV
                     NIIKGASLIIFPHKYPAWLVLEVGADKPGDILSVSKWLKSDVVVMTRIAEIPVHIEQF
                     QNQKEVVKEKSHLIRTLKDGGILVLNNDDPSIMKLREKTDNRKITFGFGKGSDLLASN
                     YHIVYEEGGDEIMPRGIAFKVDYDGGNFPVRLNGIFGKQNVYTALAAFSVGVSQGLNF
                     VKMIDVLRDHSGPPGRFKPLHGNKNTLVIDDTYNSSPVALEMALQSFKEIKSKGRKIA
                     VLGDMLELGSHTEGAHINIGKIIPEICDELVTVGGKGKFFADGAQSAGMSALNIHEFK
                     DSVSAADFVAEYMKEGDSILVKGSQGTRMEKVVEAIISKEKNDTSHLVRQDREWKRK"
     gene            1512..2087
                     /locus_tag="P5056_00615"
     CDS             1512..2087
                     /locus_tag="P5056_00615"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HRY62265.1"
                     /translation="MEYEDTKKVNNEEEVTLDTDDIIIDEEEGDQMKVVKKLRERLRI
                     CEEEKKQYLDGWQRSKADYANAKKDEAKNLSELEPLIREKILSEIIPVADNFDMAFRN
                     KVSWEEVPKNWRVGIEGIYGSLVNTFSGMGLTQFGEEGEKFDPALHQAVETEGVAESE
                     DGKILAINQRGYKYKNKVLRPAKVKVGVYKN"
     gene            2251..4101
                     /gene="dnaK"
                     /locus_tag="P5056_00620"
                     /pseudo
     CDS             2251..4101
                     /gene="dnaK"
                     /locus_tag="P5056_00620"
                     /inference="COORDINATES: protein motif:HMM:NF001413.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
     gene            4197..5288
                     /gene="dnaJ"
                     /locus_tag="P5056_00625"
     CDS             4197..5288
                     /gene="dnaJ"
                     /locus_tag="P5056_00625"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HRY62266.1"
                     /translation="MSDYYKILGVEKNASKEDIKKAFHKLAHKYHPDKKGGDEKKFKE
                     INEAYQTLSDDEKRSQYDMYGKGFQGGAGAGGFSGFGGAGFDFGDIFRNGGAGVEFDA
                     SNVEDLFSTFFGGGRSTRGKSRRGRDISADILISFHDSIFGIERKLNISKVGNCDTCG
                     GSGAKKGSEMVRCKTCNGAGRIIENKRSFLGTFATERVCDKCFGAGSTPKENCPLCKG
                     EGVLKKTEEITVKVPVGIEDGQMIRFSGRGEAVPHGIPGDLYVKIHVEKHPVWRREGE
                     NLITNMEIRLTDALLGATHKLKTLEGETDIKIPAGFAFGEILRIKGKGVPNERGGRGD
                     ILIHISIKMPKKLSGKEEELVGKLKEAGL"
     gene            5304..6350
                     /locus_tag="P5056_00630"
     CDS             5304..6350
                     /locus_tag="P5056_00630"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine/phosphatidylglycerophosphate/
                     cardiolipin synthase family protein"
                     /protein_id="HRY62267.1"
                     /translation="MKYKFFNNSEDTWLAMFEAIKGATESIYWESYIFSNDTSHYLNF
                     FKLLRDKASVGVHVVIVLDGFGSLWFSMNEELLSELKRHGVEVLFWNDWFHRIHKKIL
                     IIDEEVAFLGGVNIAHSFKKWQDLHIKISGKKIVKSIVRSFANSYYYGGGRDGRLVSL
                     KRGWKTMKAEIWLLDHFPNAGKFLLKRYYTEKISLATERVTIVTPYFAPKKWLTDLLK
                     KTVERGVEVEVLIPRSGDLRVARISNNIFASHLSQYGIKFYLMNHMNHAKALVVDNRV
                     GLLGSNNIDSQSFDFNIEASLSFEKEEMVNDLRRIVDGWKKDSVLFENGKHNHKWYYR
                     PLEIIFSFIAQIIG"
     gene            complement(6389..7807)
                     /gene="cysS"
                     /locus_tag="P5056_00635"
     CDS             complement(6389..7807)
                     /gene="cysS"
                     /locus_tag="P5056_00635"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012584040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HRY62268.1"
                     /translation="MIKLYNSLTRKKEVFNPIATGSVKMYACGPTVYDRAHIGNFRTY
                     IMEDVLRRMLKYNGLNVLYAQNITDVGHLVGDGDEGEDKMEKGARAAGKTAWEIAKFF
                     TEEYFKDSRRLNIADPNVSARATDHIKEQIEMVKKLEELGFAYKTSDGIYFDTSKSTN
                     YGEIARLDKEGLKEGARVEKNPEKRNDTDFALWKFSPKDKKRQMEWDSPWGVGFPGWH
                     IECSAMSTKYLGEPFDIHAGAMDLLPVHHTNEIAQNEAVFGHKTVNYWIHGAFLLVNG
                     GRMGKSLGNAYTLSDLEQRGFDPLAFRYFTFNAGYRQPLNFTWQALEGSAKAYENLIQ
                     KISDLMPDNPGINTKNINEEYAVKFKNLVSDDMSMPEALSLVWEMMKDKNIPNDIKLN
                     TITKFDEVLGLSLTEKAEELKNKTSNIPEQVKILISERETARLNKEWKRSDEIRAQIL
                     KMGFEIKDTGEGTKITPVGKFV"
     gene            complement(7809..8717)
                     /locus_tag="P5056_00640"
     CDS             complement(7809..8717)
                     /locus_tag="P5056_00640"
                     /inference="COORDINATES: protein motif:HMM:NF013694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HRY62269.1"
                     /translation="MISIKKHNSLIWVDLESPTTEELESVGQEFNINQHTLRELKYPS
                     DKPKVDVFNNFLYLILHFPKYGYGSKRDSIEIDFVLGKDFLITAHYTPSDPINSFSKA
                     FETSSTLNKFDLESGGQIFFYLIKEIYKNTEEELIYIEGKLQEIEGTIFSENGEKILE
                     NLSRLNKELIDFRRIMRGHKEVFDSLEVSAAEFFGPQFSHYSNTLMGNRERVWNILEG
                     NRETLLDLRQTSDSLFSARTNSIVRTLTILTFISIPISLIANIMGVNAENIPIVGHKF
                     DFWLIWILMSAIAAVMLIFFRFKKWL"
     gene            8960..9397
                     /locus_tag="P5056_00645"
     CDS             8960..9397
                     /locus_tag="P5056_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62270.1"
                     /translation="MGYPFRRKYEEDARAMMISIDMELIKPGCSESRRLALHKTRKLI
                     QCFVRYKKSIEDRIIFLQYDAYSVGESFGSLADDELFSVLNKISLGYTGDDMDLFVQL
                     DEIDTSLGEIERRIVLKHMSDEEKRKQLLDFITKENKRRDRLK"
     gene            complement(9424..10317)
                     /locus_tag="P5056_00650"
     CDS             complement(9424..10317)
                     /locus_tag="P5056_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62271.1"
                     /translation="MRKNKGASLVMILVLIAFIAIPYFYGNRNTKEATTTEETQTSSS
                     WLTVYNPRENAVSETTDNTNIEKPAPTKTAPIVKEVVVDPNQSKYAKKIQISSVSGSS
                     SYVKNESMRISNVGDESVPISGFVIETYEKETFKIPKGYELPGTLSSVEDNIVLKSGD
                     YLDIYVGLQDRKINFRENLCTGYFDQFSDFGSRLNHSCPKIDISGMLDFSDNCIKILN
                     DIPKCKIFDSKNIVENDCNDFANAHYNYVGCVKDFSSKTNFYSKRWLAWMQRDTEFFR
                     NDRGLITLRDTEGKTVATYSY"
     gene            10648..11277
                     /locus_tag="P5056_00655"
     CDS             10648..11277
                     /locus_tag="P5056_00655"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRY62272.1"
                     /translation="MIITYYGGLCTKAQIGDVSVSFNPPLRSDGKTPRFSSSVALFST
                     KEKDQNFSQGGSGEVFIIDGPGEYEVGGIFIKGVLVEKEVGGKKKMNTIYSVLFDDIN
                     LCHLGAISSADIKPEVLEKLGTIDILFTPVTGGDLATPSVAGKLATAFQPHVVIPMYG
                     TDKGEDNASLKQFLKEEGAEDLKPVDKLTIKKKDIESKEEDVVVLSPTS"
     gene            11290..11538
                     /locus_tag="P5056_00660"
     CDS             11290..11538
                     /locus_tag="P5056_00660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62273.1"
                     /translation="MFGLIEKLRSLPEQQRQKIAIATVTFLMLIVVSLWVANLNSVLP
                     AGADSGAVVSTLSPFKIFSDKILYIKDALSEKINLIKN"
     gene            11544..14042
                     /gene="gyrA"
                     /locus_tag="P5056_00665"
     CDS             11544..14042
                     /gene="gyrA"
                     /locus_tag="P5056_00665"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006317201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HRY62274.1"
                     /translation="MAKSKNNKKDAGAHYHGDVPGDAAKVEPRILKTDIVPEMKTSYL
                     DYAMSVIVSRALPDVRDGLKPVHRRILYSMYDLGLTASAKFRKSAMIVGDVLGKYHPH
                     GDSAVYDAMVNLAQEFKTRYPLVYGQGNFGSIDGDSAAAYRYTEAKMAKITSEILNDL
                     EKDTVDFVPNFDNTRKEPSVFPSTIPTLLLNGTLGIAVGMATKIPPHNLGELMDALVY
                     LADNSEATTEDLFKFIKGPDFPTGGIAFNEKDIIRASATGKGGVVVRGEAEIIETKSG
                     SSQIIISSIPYQVNKSEMIIKMAELVKEKKIEGIRDIRDESTEDIRIVIELKSGTYPE
                     QVLNALYKYTDLEQTFNYNMLALVDGVPKTLSIKNILEEFIAHRKIVVKRRTQFDLKK
                     AEEREHILLGLSKALDHIDEVIKIIKKSKDTADASANLMKTFKFSEIQAAAILDMKLQ
                     KLAGLERKKIEDELKSIQALIKELKALLASTAKILQVIKNEFLAIKEKYGDERKTKIM
                     KRGVADINVEDLIPDDENVLVLTSGGYVKRTDPGEYRAQKRGGVGVVDLNTKEEDFIT
                     IFLTASNHSDLLFFTDKGKAYQIKMYDIPEGKRATRGKSIMNFLSLADDEKVTSVLAM
                     PKELKNQKTALLMITKMGVVKKVSADSFKDVRRSGIIAIRLQEDDQLMATRFVEAGDS
                     IILITTDGQSVRFKESDAREMGRAAAGVRAIKLKKKDSVVGADVIKKDDKDSRLLVMS
                     ENGFGKMSNIKEYKIQKRGGSGIKTAKVTAKTGQIMVGKVITEAEEELIAISKNAQVI
                     RINIGEVPVLGRQTQGVRIMRLREDDSIASLTCL"
     gene            14139..14666
                     /locus_tag="P5056_00670"
     CDS             14139..14666
                     /locus_tag="P5056_00670"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRY62275.1"
                     /translation="MAFSEPAENVKNLDLKEGYKIADFGAGSGHYTLAAAKRVHARGQ
                     VFAIDVQKDILPKIQSAARDEGVHNVQILWGDVEKPGGSKLRDNMVDVVMITNMLFLS
                     EKKEDIAREALRILRPGGQVMVVDWSDSYGGLGPKSGHVVLEEEAKKIFENVGFKYGK
                     SFYAGDHHWGFVATK"
     gene            14692..15999
                     /locus_tag="P5056_00675"
     CDS             14692..15999
                     /locus_tag="P5056_00675"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRY62276.1"
                     /translation="MKSSTFQYILLVIFGFLGVFGVLVFAGIIPIGGEGATYEGAVTL
                     WGSVPERDMDLVLSDFNKENEDLFTLNYVYKDKQTFENDLINALAKGEGPDLVMITND
                     LIIKQGDKLLTIPFETYPERTFKDTFVEAGEIFLTKDGTLALPLYADPMIMYWNRDLF
                     TNAGLVTYPKTWTDFLNISEKLTKKDNKGNVFQSAVAMGSYKNISNAKDILTTLFLQV
                     GDNIISKNTGVGDTLSKLYNSTLGGTSRGAGSAIDFFVEFSNPAKSAYSWNPSLSNSK
                     SLFSEGKLAVYFGKASEYYGIKANNPHLNFDVALMPQREDSSLKVTSADVIGIAVVKA
                     SEKQGTALRALYSLTGNNYGKIISGLLPLPSLRRDVLATAEKDPVLSVFNSSAIISRT
                     WYDPDASASDIIFRSLVEAVNSGRQSASEAIRDAQIKFLEYVK"
     gene            15989..16390
                     /locus_tag="P5056_00680"
     CDS             15989..16390
                     /locus_tag="P5056_00680"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62277.1"
                     /translation="MLNKYFRGQFIFAFLLSSLPIVTLAADKQTLGDILSCGGEPCRL
                     SNILSIIQALNRYIAFTLLPIVATLAFLYAGFIMVVGTGNPKEVSKGKEIISNTIYGI
                     IIVFAAYYIINLIVSGLAGGTADTRATEFLQ"
     gene            16452..16823
                     /locus_tag="P5056_00685"
     CDS             16452..16823
                     /locus_tag="P5056_00685"
                     /inference="COORDINATES: protein motif:HMM:NF016820.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrbC/VirB2 family protein"
                     /protein_id="HRY62278.1"
                     /translation="MKAKIFYKILINATVLSVLALPFFIYADAPGVGSDSLNPLGSNT
                     GSLEQFVTNIIKVLLNIAIPITALAIVYIGFWFVTAKGNPGELSKAKDALLWTLVGFA
                     ILLGARAIVGIISSTVTGLGA"
     gene            16833..17207
                     /locus_tag="P5056_00690"
     CDS             16833..17207
                     /locus_tag="P5056_00690"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62279.1"
                     /translation="MKDLNKIIIAGISFFPMVAFAVDFKGFLGNIMSQLNILPAFLLA
                     LAVVYLLWNIVKYVQSGDPKKMAEAREVITYSIIFIFVMVSVWGLVDLLVNTFNLDGG
                     GGSLPGGTYQGDVNFPTNSLPS"
     gene            17262..17612
                     /locus_tag="P5056_00695"
     CDS             17262..17612
                     /locus_tag="P5056_00695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62280.1"
                     /translation="MKKVIGFGLSLVPFVAFAQASTGVSGLITKFGAILNQLVPLFTG
                     LAVVWLLWNIVNYIQAGGGDTKAREAARDAIVFSIILIFVMVSVWGLVGIIGETIGIT
                     DGGKAATPVELPSI"
     gene            17623..17862
                     /locus_tag="P5056_00700"
     CDS             17623..17862
                     /locus_tag="P5056_00700"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62281.1"
                     /translation="MAFVENVKSQIIEPLMIFLLGISVLYFLYGVFEFMYTGDVKKMD
                     EGKKHILWGLIGLFIIVAVYGIMGAVGDTVTSLTQ"
     gene            complement(17964..18695)
                     /locus_tag="P5056_00705"
     CDS             complement(17964..18695)
                     /locus_tag="P5056_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62282.1"
                     /translation="MRAIPKWIWAIAALLVLAMATCLYLLLQASSSLVGGYTSYLVYG
                     VLLTVAIILIRKYAPIWWAVLATPRPPIKWDWVKEWAWFIAKITLLLAILFYGYKNWG
                     AIWSNTSPIVSQISSCLVPAKKPRPPEGWVMSWKAAKGDNARDPNKTDGSYLVTITIH
                     TDERLCFVLHYKDWGRARVAEFFGDRTGDRRQRIYVGTWSQGEEGDRDRDYGTWRLKE
                     YGATSFAGKVLDHRGIEFDSTPEVR"
     gene            complement(18679..19950)
                     /locus_tag="P5056_00710"
     CDS             complement(18679..19950)
                     /locus_tag="P5056_00710"
                     /inference="COORDINATES: protein motif:HMM:NF013324.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HRY62283.1"
                     /translation="MDAIILFLFCVAVSIGIFYLLGMAVLKMAEKDICFTKNVSGWVK
                     AVVAGDEVVKWIGWIPCYHVSSETGEILSLDGKDVEVPKGTSEEDAKKMIEKEKTRIM
                     DIVEKDRGLLDDVLKNFGISFVGLWPYRKTYEYKFKWTEWVKRANAAKYEPWPREDKT
                     KHVRFRFPYFIDITEAETKGKVTIDVTLLVTIRIRNASKALFKSGGEWLANVTAAVQS
                     ATRDWIGNNTLEDITKTQHESGGSGFVSYVMSLNSSEKGNKGLIETLGVEIDMVNFLS
                     YEISGDGKERIQKATTEQYVAEQEAAAKKARADGDADATIKLAEAEARAIEVKGDAKA
                     KAMEKMLKAVHSHPGGVQIEIADRIANGIQNSKLTTLVAGEGAHPLLQIMGNQLPQER
                     ERPRDDHSRQERPNKRRDRQTPEGEKNESDS"
     gene            20199..20279
                     /locus_tag="P5056_00715"
     tRNA            20199..20279
                     /locus_tag="P5056_00715"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20232..20234,aa:Leu,seq:tag)
BASE COUNT         5803 a         3933 c         4947 g         5842 t
ORIGIN      
        1 gccatttata cgataatgag cggttttttt gatactagga agagcgagaa aagttttaac
       61 agtgagatag gtctccctct tactattctc ggattaaaaa atgcatggac agatccgctt
      121 gcttggttag tgaatatcat aaagggcgcg tcgctcataa tttttcctca caaatatccg
      181 gcgtggctcg tgcttgaagt cggcgccgat aagccgggag atattttgag cgtatccaag
      241 tggctaaaga gcgacgttgt tgttatgaca aggattgccg agataccggt acatatagag
      301 cagtttcaga atcagaaaga ggtcgtgaaa gagaaatctc atcttataag aacattgaaa
      361 gacggcggaa tattggtttt gaacaatgac gacccttcga taatgaaatt aagggagaag
      421 actgataacc gaaaaataac tttcggtttt ggaaaaggct ccgatctttt agcttctaat
      481 tatcacattg tatatgagga gggaggggac gagataatgc caagaggtat cgcattcaag
      541 gtagattatg atggcggtaa ttttcccgta aggttgaacg gaatattcgg caaacaaaat
      601 gtttacacag cgctcgccgc tttttctgtc ggggtgtcgc aaggtttgaa ttttgtaaaa
      661 atgatagacg tgctccggga ccacagcggt cctccgggaa gatttaagcc gcttcacggc
      721 aacaaaaaca cgctagttat agatgacaca tacaattcct ctccggtggc tcttgagatg
      781 gcgcttcagt cgtttaagga gataaagagc aagggaagaa agatcgccgt cttgggtgat
      841 atgctagagc tgggctctca tacagagggg gcgcacataa atattggtaa aattattcct
      901 gagatatgtg atgagctcgt cactgtgggc ggcaaaggta aattttttgc cgacggagcg
      961 cagtctgcgg gaatgagcgc tctcaatata cacgaattta aagattcggt gtctgccgcc
     1021 gactttgtgg ctgaatatat gaaagaaggt gattcaatat tggttaaggg atcgcagggg
     1081 acaagaatgg agaaggtggt ggaagcgata atttcaaaag aaaagaatga tacatcgcat
     1141 cttgtaagac aggatagaga gtggaagaga aaataaatat ttagagtaca tgtccggccc
     1201 cctgcgaggg ccggcattca gtctcggcct cattatctcg ctccgctcga gccatgctaa
     1261 ttcggcctgc gaagtctcta aatatttatt ttctcttctt gaaattatcg ctattttggt
     1321 ggtaaggacg aataaggaac aagcggacta aggtccgctt gtgcgattta atttgcaatt
     1381 tcgctatcgg ggagggtgag ggaggacgtt gacatgcgta gtctccataa gtaaaataca
     1441 gctaacaaga agaaatcatc gctcaatcga aagcggtgtt ctttaattaa gagttatatt
     1501 tcgtgatcaa aatggaatac gaagacacaa aaaaagtaaa taatgaggag gaggtgacgc
     1561 ttgacacgga tgatataatt attgatgaag aggagggtga ccagatgaaa gtggtcaaga
     1621 aactccgcga aaggctccgg atctgcgaag aggaaaagaa gcagtatttg gatggttggc
     1681 agagatcaaa ggcggattat gcgaacgcaa agaaagatga ggctaagaat ttatctgaat
     1741 tggagcctct tatccgcgaa aagatacttt cagaaataat accggtcgcc gacaacttcg
     1801 atatggcgtt tcgtaacaaa gtgtcgtggg aagaagttcc aaagaattgg agggttggca
     1861 ttgagggtat atatggaagt ctcgtaaata ctttttccgg aatggggctc acgcagtttg
     1921 gagaggaggg agaaaaattc gatccggcct tacatcaggc ggttgagacg gagggtgtcg
     1981 cagagagtga ggacggaaag atattagcta tcaatcagag aggatataaa tataaaaata
     2041 aagttttaag accggcgaaa gtgaaggtgg gagtgtataa aaattaaaga agaaagaata
     2101 aggaagcagg aattaaaaaa ccagtgtcat tccgagcttg tcgaggaatc gcttataatt
     2161 cagaaatgaa gagatctctc gacaagctcg agatgacaac aaaaaataaa attcgtaatt
     2221 cgtaattatt aattgataat tgattaaact atggcaaaaa tattaggaat agatttgggt
     2281 acaacttttt cggcgatggc tgttgttgag ggtggtcagc cgaaaatcat agagaattca
     2341 gatggggcaa gaacgactcc ttctatagtt gccgtgtcta aggcgggaga aaggatcgtc
     2401 ggccagctcg cgaaaagaca gagtataaca aatccgcaga acacgattta tggcattaag
     2461 aggcttgtgg gccaccgata tgaagacaag gaagtcgaaa gagatagagg ctccgtgcct
     2521 tttgctatcg ttaaatcttc tgatggtggt gttaaggtaa agatgaacga caaagaatat
     2581 actccggagg aggtgtccgc gatggttttg cagaaattga agacagacgc ggaggccaag
     2641 attggtgaga agatcgagga ggctgttatt acagttcccg cttattttag tgattcacag
     2701 agaaaggcca caaaggatgc cggagaaatt gccggtttca aggtaagaag gatcttgaat
     2761 gagcctactg cggcggcctt ggcttatggt tttgacaaga aaaagaacga gcaaattgcc
     2821 gtttatgact tcggaggagg aacatttgat gtttctgtcc ttgatgtcgg ggatgatgtt
     2881 atcgaggtga agtccactga cggagatagc cacatgggcg gagaagacat agaccagaag
     2941 ataatgaagt gggtcgctga ggaattcaaa aaagagagcg gtattgatgt ttccgccgat
     3001 ccgcttgcaa agcagaggct aaaggaggcg gctgaaaaag cgaagcatga actttctact
     3061 gttaaggagg tggaaattaa tatcccattc attacttctg acgccacagg tccaaagcac
     3121 cttcttttga agatgaccag agcaaagctg gaagagctcg caaaggaata tattgataga
     3181 tccattgaaa taacaaaaag agcggtagag gcatccggcc tgaagctttc tgaagttgaa
     3241 gaagttattt tggttggagg tcagactaga atgccggcca ttattgacgc cgtaagaaac
     3301 ctttttggta aggaaccaaa cagatctatt aacccggatg aagttgtggc tgttggagcc
     3361 gctattcagg gaggtatttt acagggagaa gttaaagacg tgcttcttct cgacgttatc
     3421 ccgctttctc tcggtatcga gacgttggga ggagttgcga caaggcttat agaaagaaat
     3481 acaacgatac cgacatcaaa gtcgcaggta ttttctaccg ccgcagacag ccagacatct
     3541 gttgatattc acgttgttca gggagaaagg cctatggttg cagacaacaa gactctcggt
     3601 aaattcaccc ttgaggggat tccgcctgcg ccacgaggcg tgcctcaggt agaggtaact
     3661 tttgatattg atgtgaacgg aattttgagt gttaccgcca aagagaagac gacaggaaag
     3721 gcccagtcta taaagataga ggctagaggc ggtcttaagg agacggatat cgagagaatg
     3781 aagaaggagg ccgagttaca tgctgaggaa gacaaaaaga aaaaagagga gattgatgtc
     3841 agaaatcacg ccgaaggtgt tatcgtaacc gcagaaaagg cactaaagga cgctgagggt
     3901 aaaattacag atgagataaa gagcggcgtc accgcaaaga tagacgaact caaaaaagtt
     3961 aaggacggag gagatataga gactatcaag aaggcctcgg atgctctcgt tgttgaaatg
     4021 caaaagatcg gcgaatatat gatgaagcag aatcagggcc aggccgcagg tgccgaagaa
     4081 ccgaagaaag atgataatat cagagacgct gaataaataa ttatcaatta cgaattaata
     4141 attacgaatt tattttaaaa taatttgtaa ttcgtaattg taaatttcta attaatttga
     4201 gtgattacta taaaatttta ggcgtagaaa agaatgcctc caaggaggat attaaaaagg
     4261 cgttccataa gcttgctcat aaataccatc cggacaaaaa aggaggcgac gaaaaaaaat
     4321 ttaaagagat aaatgaggct taccaaaccc tgtctgatga tgaaaaacga agccaatatg
     4381 atatgtatgg caagggtttt cagggcggcg caggagccgg cggattctcc ggatttggag
     4441 gggccggttt tgattttggt gatattttcc gaaacggcgg agccggcgtt gaatttgatg
     4501 cttccaatgt ggaagatctg ttcagcacat tttttggagg cggacgctct actcgcggaa
     4561 aatccagaag gggcagagat atctctgccg atattctcat ttctttccac gattctattt
     4621 ttgggattga aagaaaattg aacataagca aggtcggcaa ttgcgatact tgcggcggtt
     4681 ccggagcaaa aaagggtagc gagatggtga ggtgcaagac ttgtaacggc gccggcagaa
     4741 taatagagaa caaaagatct tttctgggca cctttgcgac agagagggtt tgtgataagt
     4801 gttttggcgc cggtagcacc ccaaaggaga attgtcccct atgtaagggc gagggggttt
     4861 tgaagaagac agaagaaatt acggtcaagg ttcctgtggg aattgaggac ggtcaaatga
     4921 taagattctc ggggagaggt gaggcggtgc cgcacggtat tcccggcgat ctttatgtaa
     4981 aaattcacgt agaaaaacat ccggtctgga ggagagaggg ggagaatttg atcacaaata
     5041 tggagattag gcttacagat gcgttgctcg gcgccacaca caaactgaag acgctagaag
     5101 gagaaacgga cataaaaata cccgccggtt ttgcttttgg agaaatactc aggataaagg
     5161 gcaagggagt tcctaacgaa aggggcggaa gaggtgatat acttatccat ataagcatca
     5221 aaatgccgaa gaagctttcc ggaaaagaag aggagcttgt cggtaagctt aaggaggcgg
     5281 gtttgtagtt tttggcggaa ataatgaaat ataaattttt caataatagc gaagacactt
     5341 ggcttgccat gtttgaggct attaagggtg caaccgaatc tatctattgg gaaagttata
     5401 tttttagcaa cgacacaagc cattatctaa atttttttaa gcttttgcgc gacaaggcgt
     5461 ctgtaggcgt tcacgtggtg attgttttgg acggttttgg tagcctctgg ttcagtatga
     5521 atgaagagct tcttagcgag ctgaagcgcc atggagtaga agttttattt tggaacgatt
     5581 ggttccacag gattcacaaa aaaatactca taatagacga agaagtcgct tttctgggcg
     5641 gagttaatat cgcacacagt ttcaaaaagt ggcaggattt acatataaaa atttccggca
     5701 agaagattgt taaaagcatc gttcgttctt tcgctaatag ttactattat ggagggggga
     5761 gagacgggag actcgtgtct ttgaaaagag gatggaagac aatgaaagct gaaatatggc
     5821 tcttggacca tttccctaat gcgggtaagt ttttgctgaa aagatactat accgaaaaaa
     5881 tttctttggc cacagagagg gtcacgatag ttaccccata ttttgctccg aaaaagtggc
     5941 tcacagattt gctaaaaaag accgtagaaa gaggggtgga ggtggaagtt cttattccca
     6001 gatcggggga tcttcgagtg gctagaatat ctaataatat ttttgcgtcc cacctttcgc
     6061 agtatggaat taaattctat ctaatgaatc atatgaatca cgccaaggcc cttgtcgttg
     6121 ataaccgggt gggccttctc ggttccaata atatagattc ccagagtttt gattttaata
     6181 tagaagcgag tttgtctttt gaaaaagaag agatggtaaa cgaccttaga agaatcgtgg
     6241 acggatggaa aaaggactcg gttctttttg agaacggcaa gcacaatcac aaatggtatt
     6301 accgcccgct ggaaataata ttttctttca tagcccagat aataggttaa aaaatcccct
     6361 tatggggatc ttttatttta gaaataggtt atacgaactt tcctaccggg gttattttgg
     6421 ttccttctcc cgtatctttt atttcaaaac ccatcttcag tatttgggct cttatttcat
     6481 ccgacctctt ccactctttg tttaatctgg ccgtttctct ttctgagatc agaattttga
     6541 cctgctccgg tatattagat gttttatttt ttagttcttc ggctttttca gtgagtgaaa
     6601 gccccaatac ttcgtcgaac tttgtgatgg tatttagctt gatatcgttt ggtatgtttt
     6661 tgtctttcat catttcccat acaagagaca aggcttccgg catggacata tcatcgctga
     6721 ctaagttttt aaattttacg gcatattctt cgtttatatt ttttgtgttt attccgggat
     6781 tatccggcat aagatcactt attttttgta taaggttttc gtatgctttt gccgaaccct
     6841 ctagcgcctg ccatgtgaag ttgagaggct gtctataccc tgcgttaaat gtgaaatatc
     6901 tgaaggcgag aggatcaaag cccctttgtt ctaggtcgct taatgtgtag gcgtttccga
     6961 gtgacttgcc cattctgccg ccatttacga ggaggaatgc tccatgtatc caataattta
     7021 ccgttttgtg gccaaaaacc gcctcatttt gagcaatttc gttggtgtgg tgcaccggca
     7081 ggaggtccat ggctccggcg tgtatatcaa aaggctcgcc gagatatttt gtgctcatgg
     7141 cggaacactc tatgtgccag ccggggaatc cgactcccca aggggaatcc cactccattt
     7201 gtcgtttttt gtcttttggt gaaaatttcc ataacgcgaa atcggtatcg tttctttttt
     7261 ccggattttt ttctaccctt gcgccctctt ttagcccttc tttgtctaat cgtgcgattt
     7321 ctccataatt tgtcgatttg cttgtatcaa aataaatacc gtcactagtc ttgtaggcaa
     7381 agccgagctc ctccaatttt ttcaccattt caatctgctc ttttatatgg tcggtagccc
     7441 tagctgaaac attaggatca gcgatgttga gacggcgaga gtctttgaaa tattcttctg
     7501 taaagaattt tgctatttcc cacgctgttt ttccggccgc tcttgcgccc ttttccattt
     7561 tgtcttctcc ctcgtcgccg tctcccacaa gatggccgac atctgttatg ttctgggcat
     7621 ataggacatt gaggccgttg tatttaagca tacgtctcaa cacatcctcc attatgtatg
     7681 ttcggaaatt gccgatatgc gctctatcat ataccgttgg tccacatgca tacatcttta
     7741 cagatccggt tgcaatcgga ttaaagacct ctttttttct agttaaggaa ttataaagct
     7801 taatcatttt atagccattt cttaaatcta aagaatataa gcatgacggc cgctattgca
     7861 gacattaaaa tccaaataag ccagaagtca aatttgtgcc cgactatcgg tatgttttcg
     7921 gcgtttaccc ccattatgtt ggcaataagg gagattggga tgcttataaa ggttagtatt
     7981 gttagcgttc tcacaatgct atttgttctt gcggagaaca atgagtcgct tgtttgccta
     8041 aggtcgagaa gtgtctctct gtttccctcc aatatattcc acactctttc ccgattcccc
     8101 atgagcgtgt ttgaatagtg cgagaattgt ggaccgaaga attccgcggc agacacttcc
     8161 agggagtcaa atacctcttt gtgtcctctc attattcttc tgaagtctat caactcctta
     8221 ttgaggcgcg atagattttc gagtatcttt tcgccatttt ctgagaatat tgtcccttct
     8281 atttcctgta atttgccctc tatgtagata agttcctcct cggtgttttt gtaaatttct
     8341 ttaataagat aaaaaaatat ttgaccgccg ctttccaggt cgaatttgtt cagtgtcgag
     8401 ctagtttcga aggcttttga gaagctgttt atcgggtctg atggtgtgta gtgagccgtg
     8461 attaaaaaat cttttcccag aacaaaatct atctctattg agtccctttt actgccatac
     8521 ccatatttag gaaaatgcag tatgagataa agaaaattat taaaaacatc gacttttggt
     8581 ttgtcgctcg gatacttgag ctcccttagc gtatgctggt tgatgttaaa ttcttgcccg
     8641 acgctctcta gttcttctgt ggtgggggat tcgagatcaa cccagattaa gctgttgtgc
     8701 ttttttattg atatcataaa tttatgttta tctaattata acattgaaca tttcatttcg
     8761 ccatttctgt tatgtgtaga tttgcccggt ttttgatagg ccacctgccc cgtaggccct
     8821 gttaaataat ttgtagggca aattagcaca ccgtgctatt taacagggtt ctaccggggt
     8881 tgcgtcaccg gttattaagt tgtatccttg gcctaagtat aagttctttt attattttat
     8941 ctcgaaggga gggattttta tgggataccc ttttcggcga aaatacgaag aggatgcccg
     9001 cgcaatgatg atctctattg atatggaact tataaaaccg gggtgttctg agtctcgcag
     9061 gctagctctg cataaaacca gaaaactcat tcagtgtttt gtaagataca aaaagagcat
     9121 agaagataga attatttttt tgcagtatga cgcgtattcc gtcggcgaat ctttcggttc
     9181 tttggccgac gatgaattgt tttccgtgtt aaataaaatt tcgctcggat acaccgggga
     9241 tgatatggac ctttttgttc agcttgatga aatagacact tctctcggag agatagagcg
     9301 ccgcattgtt cttaaacata tgagcgatga agaaaaacgc aaacagctct tggattttat
     9361 tactaaagag aacaagcgca gagacagact aaaatagcga ccttatcagg gccgctattt
     9421 cttttaatat gaatatgtgg cgacggtttt tccctcggta tctcttagcg ttatgagccc
     9481 cctatcattt ctaaaaaatt ccgtgtcgcg ttgcatccat gctagccagc gcttcgaata
     9541 gaagtttgtt ttagaactaa aatcttttac acacccgaca tagttataat gcgcattggc
     9601 aaaatcgtta caatcatttt caacaatgtt ttttgagtca aaaattttac actttggtat
     9661 gtcatttaag atttttatgc aattgtcgga gaaatcgagc attccggaaa tgtctatttt
     9721 cgggcaggag tggttcagcc tgctcccaaa gtcgctgaat tgatcaaagt aaccggtgca
     9781 aaggttctct ctaaaattta tttttctgtc ctgcaacccc acatatatgt ccagatagtc
     9841 tcccgatttc aagacgatat tatcctcaac cgaactaagc gttccgggga gttcatatcc
     9901 tttgggaatt ttgaacgttt ctttttcgta agtttctatt acaaaacctg atattgggac
     9961 tgattcgtca ccgacattgc ttatccgcat agattcgttt tttacatagg agctagaacc
    10021 gctaaccgag cttatttgaa tctttttggc gtattttgac tgattcggat caacgacgac
    10081 ctcttttacg attggggcgg tttttgtcgg agcgggtttt tcaatatttg tattgtcggt
    10141 tgtttctgaa acagcatttt ccctcggatt atagacggta agccacgacg agctcgtttg
    10201 ggtctcttcc gtcgtggtgg cctctttggt gtttctattc ccataaaaat aaggaatagc
    10261 gatgaatgct ataagcacca agatcatcac cagtgaggcg cctttgttct ttctcacccg
    10321 cctaaatttt tcactattca tattgttaag gtaagataaa ttgataaata attaatatat
    10381 ttttaatagt taattaatgt tctgtttaaa atttcttgcc cgccaaaatt tatggaataa
    10441 atttaggagg gtgaagtggg taaattttga ctaagttatt tttactgctt aattttatca
    10501 ctatagagaa ttgcgcgcca ctctccttga tttaactcgg attatgtgtt ttttgtggat
    10561 aggtattctt taatttgagc ctattttttg gtatatttat gtctgttgtc tataataatt
    10621 taaggccacc tagcactaaa actaaaaatg ataattacat attatggagg gttgtgtaca
    10681 aaggctcaga taggggatgt ttccgtttca tttaacccgc cgcttagaag cgacggcaag
    10741 accccgcgat tttcttctag tgtcgcgttg tttagcacaa aagaaaaaga tcagaatttt
    10801 tctcaaggag gttccggcga ggtttttata atagacggac cgggagagta tgaagtgggg
    10861 ggcatcttta taaagggggt gctagttgaa aaagaggttg gcgggaaaaa gaagatgaat
    10921 actatttact cggttctttt cgatgatatt aatttgtgtc atctgggggc gatatcttct
    10981 gccgacataa agcccgaggt tttggaaaag ctcgggacga tagatatttt gttcacaccc
    11041 gttacgggag gagatttggc gacaccttcg gtcgccggca agctagccac ggcatttcag
    11101 ccgcacgtcg tcattcctat gtatggcaca gataagggcg aagataacgc atcattgaag
    11161 cagtttttga aagaagaagg agcagaagat cttaagcctg tagataaact gacaataaag
    11221 aaaaaagaca ttgagagtaa ggaggaagat gtggtggttt tgtcgccaac gtcgtaatta
    11281 gttaatctaa tgttcggact aatcgaaaaa ttaagaagcc ttcctgaaca gcaaagacaa
    11341 aagattgcga tagccaccgt tacctttctg atgctgattg ttgtgtcgtt gtgggtcgcc
    11401 aatctaaatt cggttcttcc ggcgggagca gacagcggcg cggtagtttc aactctttca
    11461 ccatttaaaa tattttcgga taaaatttta tatattaaag acgctctctc cgaaaaaata
    11521 aatttaatta aaaattaaac aacatggcca agtcaaagaa taataaaaaa gatgccggag
    11581 cgcactatca tggtgatgtc ccgggggatg ccgcaaaggt agagccaaga atactaaaga
    11641 ccgacatcgt gccggagatg aagacttcct atttggacta tgccatgtct gttattgttt
    11701 cccgtgcgtt gcctgacgta agagacggtc ttaagcctgt tcaccgaaga attttgtatt
    11761 ctatgtatga tttgggcctt accgcttcgg ctaagtttag aaagtcggcg atgatcgtcg
    11821 gagatgttct tggtaaatat cacccgcatg gagattctgc ggtttacgat gcgatggtga
    11881 acttggcgca ggaattcaaa accagatacc ctctcgtata tggtcagggt aacttcggtt
    11941 ctatcgacgg ggatagcgcg gcggcttata gatacacaga ggccaagatg gccaaaatca
    12001 cttccgagat attgaacgac cttgagaaag atacggtaga ttttgtgccg aactttgaca
    12061 atacaagaaa agagcctagc gtttttcctt ccacaatacc gacgcttctt ctaaacggga
    12121 cactgggtat tgccgtaggt atggccacga agatccctcc tcataatctc ggggaactaa
    12181 tggacgccct ggtgtatttg gcggacaaca gcgaagcgac gacagaagac ctttttaaat
    12241 ttatcaaagg acccgatttc cctaccggcg gaattgcgtt caatgagaaa gacatcataa
    12301 gagcctccgc cacaggcaag ggaggcgttg tcgtgcgcgg tgaggcggaa ataatagaaa
    12361 caaaatcagg atcatctcaa attataattt cttccattcc gtatcaggtg aacaagtctg
    12421 aaatgataat taagatggcc gagcttgtga aagaaaagaa gatcgaagga attagggata
    12481 ttcgagacga gtccacggaa gacataagaa tcgttatcga gctgaagtcg gggacttatc
    12541 cggaacaggt tttgaacgct ttatacaaat acactgattt agagcaaacg tttaactata
    12601 acatgctagc ccttgtggac ggcgtgccga aaaccctttc gataaagaat attttagaag
    12661 aattcatcgc gcacagaaag atagtcgtta aaaggcgaac acaatttgat ctcaagaagg
    12721 ccgaagaaag ggagcatatt ttattgggtc tcagtaaggc gctcgaccat atagatgaag
    12781 tcataaagat catcaagaaa tcaaaagata cagctgatgc gtccgcgaac ctgatgaaga
    12841 catttaagtt ctcggaaata caagccgccg caatcttgga tatgaagctt cagaagcttg
    12901 ccggcctcga aagaaagaag atcgaggatg aactcaagtc aatacaggct cttattaagg
    12961 agctcaaggc tcttcttgct agcaccgcaa aaatattgca ggtcataaag aacgaatttt
    13021 tggcaatcaa agaaaaatac ggtgacgaaa gaaagaccaa aataatgaag aggggtgtcg
    13081 ccgacataaa tgtcgaggat cttattcccg atgacgaaaa tgttttggtt cttacgtccg
    13141 gcggttatgt taagaggaca gacccgggag aatatcgtgc ccaaaagagg ggcggtgtcg
    13201 gtgtcgtgga cttaaatacc aaggaagagg actttattac catattcctt accgcctcca
    13261 accacagcga tcttctgttc tttaccgaca agggtaaggc atatcagata aaaatgtatg
    13321 atattccgga gggtaagcgc gccacaagag gcaagtctat aatgaacttc ctttctttgg
    13381 ctgatgacga aaaagttacc tcggttttgg ctatgccgaa agagctcaag aatcagaaaa
    13441 cggcgctact tatgataacc aagatgggtg tcgtgaagaa ggtttccgct gacagtttca
    13501 aagacgtcag aagaagcgga attatcgcaa tcaggcttca ggaagacgac caacttatgg
    13561 ccacaagatt tgttgaggcg ggagattcta taatacttat cactacagac ggtcagtctg
    13621 tcagattcaa ggagtctgac gcaagagaga tgggccgtgc cgcggcgggt gtgcgcgcca
    13681 tcaagctcaa gaaaaaggat tccgtagtcg gtgcagatgt tattaagaaa gacgataaag
    13741 actctaggct ccttgtaatg tccgagaatg gttttggaaa gatgagcaat ataaaagaat
    13801 ataagataca aaagagaggc ggctcgggca taaagacggc taaggtaacg gcaaagaccg
    13861 gacaaataat ggtaggaaag gttattacag aagcggaaga agaacttatc gccatttcta
    13921 agaacgcaca agttataaga atcaatatcg gcgaagtgcc ggtgttgggt aggcagacac
    13981 aaggagtcag aataatgaga ctccgcgaag atgatagcat agcgtctctt acttgcttat
    14041 agtctaagat atctttttga aaacagcgcc ttgtggacgc tgtttttggg gataaatagc
    14101 gttatctttt tatttttcag gtgctataat tacgcagtat ggcttttagc gaaccggcgg
    14161 aaaatgtaaa aaatcttgac cttaaggaag gctacaaaat agctgatttt ggggcgggta
    14221 gcggccacta tactttagcg gcggcaaaga gagttcatgc gagaggccaa gtattcgcaa
    14281 tcgatgtcca aaaagatata ttgccgaaaa tacaatctgc ggccagagat gaaggtgtcc
    14341 acaacgtgca gattttgtgg ggagatgttg aaaagccggg cgggtccaag ctccgcgaca
    14401 atatggtgga tgtggttatg atcacaaata tgctttttct gtcggagaaa aaagaagata
    14461 tcgcaagaga ggcgcttaga atattgagac cgggcggaca agttatggtt gttgattggt
    14521 ccgattctta cggagggctt ggcccgaagt cgggtcatgt cgtgctagaa gaagaagcaa
    14581 aaaaaatatt tgagaacgtc ggtttcaaat acggaaaaag tttttatgcg ggggatcatc
    14641 actggggttt cgtcgcaaca aaataattat ttttaatttt ttagatcaaa gatgaaaagt
    14701 agcactttcc aatatattct attagtaatc ttcggttttt tgggagtttt tggggtcctg
    14761 gtgtttgccg ggattattcc gattggagga gagggtgcga cttatgaagg cgcagtgacg
    14821 ctatggggtt cagtcccgga aagagatatg gatctggtgc tttctgattt caataaggag
    14881 aacgaagatc tttttaccct gaattatgtc tacaaggata agcaaacttt tgagaatgac
    14941 cttataaatg cgctagctaa gggtgagggg ccggaccttg ttatgattac gaatgacctt
    15001 atcatcaaac agggtgataa attgttgacc attccttttg aaacatatcc ggagagaact
    15061 tttaaagata cttttgttga agcgggtgag atatttttaa ctaaggacgg aacgcttgct
    15121 ttgcctcttt acgcagaccc gatgatcatg tattggaaca gggatttgtt cacgaatgcc
    15181 ggtcttgtaa catacccaaa gacctggact gattttctaa atatttcaga aaaacttaca
    15241 aaaaaggaca acaaggggaa tgtatttcag agcgcggttg cgatggggtc ttacaagaac
    15301 atatcaaatg ccaaagacat tcttaccacg ctatttttgc aggttggcga taatataatt
    15361 tctaaaaaca cgggtgtggg tgacacgttg agcaaacttt ataattccac gcttggagga
    15421 acaagcaggg gggccggttc agctattgat ttctttgtag aattttctaa tcccgcgaag
    15481 tcggcttatt cttggaatcc ttcgctctca aattcaaaga gtctatttag tgaagggaag
    15541 ctcgctgttt attttggaaa agcaagcgag tattatggca tcaaggcgaa taatccacac
    15601 ttaaattttg atgtagcttt aatgccccag agagaagatt cttcgctaaa ggtaacttcc
    15661 gcagatgtta tcggtatcgc ggtcgtgaaa gcgagcgaga agcagggcac cgcccttagg
    15721 gctctttatt ctctgaccgg caataactat gggaaaataa tttccggtct attgcccctt
    15781 ccttctttaa gaagagatgt cttggctact gcagagaaag accctgtttt gtcggtgttt
    15841 aatagttcgg ccataataag taggacgtgg tatgatccgg acgcttccgc aagtgatata
    15901 atattcagat ccttagttga ggctgttaat tcaggaaggc agagtgcctc cgaagccatt
    15961 agagatgccc aaattaaatt tttagaatat gttaaataaa tattttaggg gacaatttat
    16021 ttttgctttt ctgctttcat ctctgccgat agttaccctc gccgccgaca aacaaaccct
    16081 cggagatatt ttgagttgcg gaggggagcc gtgcaggctt tcaaatattc tttcaattat
    16141 tcaggcgcta aatagatata tcgcgtttac tcttctcccg attgtcgcca ccttagcgtt
    16201 tttgtatgcg ggatttataa tggtcgtggg aacgggtaac ccgaaagaag tttcaaaagg
    16261 aaaagagata attagtaata caatatatgg tataattata gtcttcgccg cctactatat
    16321 aattaatcta attgttagcg ggcttgcggg gggaacagca gatacaaggg ctacggaatt
    16381 tttacaataa attcaccctg tttagtaatc ccgttgagta aaattttatt tagaaataaa
    16441 atttaaatta aatgaaagca aaaatatttt ataaaatact tattaacgcc accgttcttt
    16501 ctgttttggc tttgcctttc tttatttacg cagatgctcc gggtgtcggc tctgacagcc
    16561 ttaaccctct tggcagtaat accggaagcc ttgaacaatt cgttacaaat atcataaagg
    16621 ttcttctaaa tattgcgatt ccaatcaccg ctcttgcgat agtttatatt ggtttttggt
    16681 ttgtaacagc caagggcaat cccggggagc tcagtaaggc aaaagatgcc cttctttgga
    16741 cactggtagg gtttgcgata ctacttggcg ccagggcaat agttggtata attagttcta
    16801 cagtaactgg ccttggagct taaaataaaa aaatgaaaga tttaaacaaa ataattattg
    16861 ccggaatatc ttttttcccc atggtggcct ttgcggtgga ttttaaggga tttctcggaa
    16921 acataatgag ccagcttaat atattaccag ccttccttct tgcattagcg gtcgtatatt
    16981 tgctttggaa tattgttaag tatgtccagt ccggagatcc aaaaaagatg gcagaggcca
    17041 gagaggtaat cacatacagc attatattta tctttgttat ggtttctgtt tggggcttgg
    17101 tagatttgct ggtaaatacc tttaatttgg atggaggagg gggaagcctt cccggcggga
    17161 cgtatcaagg agacgtaaat tttccgacaa atagtttacc ttcgtaatta ttagaaatta
    17221 aaaataggtc gttaaaatta ttagtttaaa aaaataaaaa aatgaagaaa gttataggtt
    17281 ttggtttgag tttggtgccg tttgtcgctt ttgctcaggc atcaacagga gtttccggtt
    17341 taattaccaa gtttggggct atattgaacc agcttgttcc tctttttacc ggattagccg
    17401 ttgtttggct tctctggaat atcgttaatt atattcaagc cgggggagga gatacaaaag
    17461 caagagaagc ggccagagat gcgattgtct tctcaataat cctcatcttc gtcatggtct
    17521 ccgtttgggg cctagtcggt attatcggcg agacgattgg tatcaccgac ggtggcaagg
    17581 cggctacccc tgtagaactt cctagcatat aaaaaataaa agatggcctt tgttgaaaat
    17641 gtaaaatcgc agatcataga gccgctcatg attttccttc ttggaatatc agtcttatat
    17701 ttcctgtatg gcgtatttga atttatgtat acgggggatg tgaaaaagat ggatgagggc
    17761 aagaaacaca ttttatgggg gcttatcgga ttgtttataa tcgtggcggt ttacggaata
    17821 atgggagctg ttggagatac ggttacgtct ctcactcaat aaaacttatt cattcggatg
    17881 tagaacgccg aatgtgaaag ttttataaaa aataaaaaac cctcggacga tgtccgaggg
    17941 ttttggtttt ttacccgtaa cagttaacgc acttcggggg ttgagtcaaa ctcgataccc
    18001 cgatgatcta ataccttccc ggcgaaggaa gtggcgccgt attctttcag cctccacgtt
    18061 ccgtagtctc gatctctgtc tccttcctcc ccctgagacc aagtgcccac atagatcctt
    18121 tgtcttcgat cgccagttcg atccccgaag aattcggcga ctcgagctcg gccccaatct
    18181 ttatagtgca agacaaagca gagcctttcg tcggtgtgaa tggtgatggt gacgaggtaa
    18241 gagccgtccg tcttattcgg gtctcttgcg ttgtcgccct ttgcggcttt ccagctcatg
    18301 acccagccct ctggcggtcg tggctttttt gccggcacca aacatgacga gatctgggag
    18361 acaatggggc ttgtgttgct ccatatggct ccccaatttt tgtatccgta gaaaaggata
    18421 gcaaggagca gggtgatttt tgctatgaac catgcccact ccttgaccca atcccatttg
    18481 atagggggac ggggagtcgc gaggacggcc caccaaatgg gggcatattt ccggataagg
    18541 ataatggcca ccgttagaag gacgccgtaa accagataag atgtgtaccc acccacaagg
    18601 ctagaactcg cttgcaggag taggtacagg caagtggcca tcgcaagcac gagaagtgcc
    18661 gcgatggccc agatccactt aggaatcgct ctcattcttt tctccctccg gcgtttgccg
    18721 atctcttctc ttgttcggtc gttcctgcct gctgtgatca tctctagggc gctcgcgttc
    18781 ctgaggaagt tgattgccca tgatctggag cagggggtgt gccccttctc ccgccacgag
    18841 cgtcgtcagt ttggaatttt ggatcccgtt cgcgattctg tctgcgattt cgatctgaac
    18901 tccgccggga tgagagtgga ctgccttgag catcttctcc attgccttgg ccttggcatc
    18961 gcccttcact tctattgctc tcgcttcagc ttcggccagc ttaatagtcg catcggcgtc
    19021 tccgtcggcg cgagcctttt tggcagcagc ttcttgctct gcgacatact gttcagtcgt
    19081 ggccttttgg atacgctctt taccgtcccc agaaatctca taagaaagga agttgaccat
    19141 atcgatctct actccaagcg tttcgataag gcccttgttt cccttctcag agctattaag
    19201 agacataacg tagctgacaa agcctgaacc gcccgattca tgttgggttt tggtaatgtc
    19261 ttccaacgta ttgttgccga tccagtcgcg cgttgcgctc tggaccgcgg cagtcacgtt
    19321 agcgagccat tctccgcccg acttaaacag ggccttggaa gcgttgcgga tcctgatggt
    19381 gacgagcaac gtgacgtcaa tcgtcacctt gcctttggtt tccgcttcgg tgatatcaat
    19441 aaaatagggg aacctaaacc taacatgctt agttttgtct tctctgggcc agggttcgta
    19501 tttggccgcg ttggctctct tcacccattc ggtccacttg aacttgtatt cgtaggtctt
    19561 gcgataaggc cacaggccga caaaagagat accaaaattt ttcaagacat cgtccaggag
    19621 acctctatct ttttcgacga tatccatgat cctggttttt tctttctcga tcattttctt
    19681 ggcgtcttct tcactggtgc ccttgggaac ctcgacgtcc tttccgtcga gggacaggat
    19741 ttctccggtc tcagaggaga catgatagca ggggatccaa ccgatccatt tcacgacttc
    19801 gtctccggcg acaacggctt tgacccaacc gctcacgttt ttggtgaagc agatgtcttt
    19861 ttcggccatt ttgaggacag ccatgcccag gagatagaag attccaattg agacggcgac
    19921 acagaacagg aacaagatga ttgcatccat ttgggacccc tttcgtgtgt tgttgcgtta
    19981 ttgttggccc gcttaaattt tcccctatag accctatgtc aataggttaa atcaatttta
    20041 tttgatcgtt gcttattggc gatacgggta tagcttaaaa aatttaggcg ggtaaatttt
    20101 acaaagacct cttttcgtgt ttgattatcc actattttgt gattatgtca agtttttgat
    20161 tgccacaata tgattaatct ggtagtcttt aaatcaatgc gcgggtggtg aaatggcaga
    20221 cacgccagcc ttaggagctg gtgccgcaag gcatggaggt tcgagtcctc tcccgcgcac
    20281 ataaaaacag gcccaaaggc ctgtttttgc gcggagcaag caatcttaaa ttgcttgcgg
    20341 agaggactcg aagcctgatt gagcattttt tatagccttt gggcgtataa aaaatccaat
    20401 caggatactg gtcgtgtaac gaccgagtcc ttattcactc atctgtaaga ttcgagtcct
    20461 ttcttatata aatgacctcc tccccgggtt ccgctccgct acccccgggg tttccttttt
    20521 cggca
//
LOCUS       DAOTEX010000006        20244 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3118
            NODE_1755_length_20244_cov_4.346476, whole genome shotgun sequence.
ACCESSION   DAOTEX010000006 DAOTEX010000000
VERSION     DAOTEX010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868142
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 20244)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20244)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 883
            CDSs (total)                      :: 833
            Genes (coding)                    :: 829
            CDSs (with protein)               :: 829
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20244
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1755_length_20244_cov_4.346476"
                     /isolate="AMR_MDS_3118"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            48..1439
                     /locus_tag="P5056_00720"
     CDS             48..1439
                     /locus_tag="P5056_00720"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HRY62284.1"
                     /translation="MKKVFIYFLGLVSLFFCFNFSLAQTATKVVKVGAFNNYPVIFKN
                     TSGSIEGLYVDLLEEIGKRENIKFDYVFGTWNDGLERIKSGEVDLLTSVAYTEDRALI
                     MEYTKNPLMTVWGDVYAPKNSDISGILELSGKKIGVLIGDINYKNFQDLLSRFGLESE
                     FVEFSSYDDVFAAVADGEVDVGVAGITFGATNESKYGLKSTGIVFGPLNLYFTTAKDK
                     NPELILTLDKYLSSWKKQDNSFLDQTKQKWFYGGLGTVIVLPDWIKQSLFALLILLSI
                     SFAFVFLLRKQVRAATEKIRAQTKDLEESELKYRSIYNNFMDVYYRADMNGIIIEASP
                     SCADVTGWSQTDLVGRPVFDISFNPEDQIVFRDKLLRDGYVNDYEIKLLKRGGSPFVG
                     SISSHMVQDEKGNPKYIEGVVRDITQRKKIEEELRRSVEELEKMNKFMVGREVKMTEM
                     KKEIDELRARMGK"
     gene            complement(1669..2073)
                     /gene="ssrA"
                     /locus_tag="P5056_00725"
     tmRNA           complement(1669..2073)
                     /gene="ssrA"
                     /locus_tag="P5056_00725"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(2173..2619)
                     /gene="smpB"
                     /locus_tag="P5056_00730"
     CDS             complement(2173..2619)
                     /gene="smpB"
                     /locus_tag="P5056_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HRY62285.1"
                     /translation="MSLIINRKYGLKYEVLEKMSAGIELLGTEVKSLKDGHGVIDAAY
                     VIIRGGEAYLVNADIPAHQPKNAPKDYDRLRNRRLLLTKKELNQLGGNTSQKGLTIIP
                     LSVYIKGRKLKLDIAIAKGKKKFDKRETIKKRETAKNIERIIKEGR"
     gene            4626..6548
                     /gene="ftsH"
                     /locus_tag="P5056_00735"
     CDS             4626..6548
                     /gene="ftsH"
                     /locus_tag="P5056_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HRY62286.1"
                     /translation="MSKKNNNKSGKNDPWKAMSQFGRSILGFILIFVLIIGLYSAYVE
                     ESKKTEDISISVLSSDIKAGLVKKITVEGDYLYADYNDEKQKISQKELETSLTETLVN
                     YGVTGEELAKVNVEVKQSSGVMFWIINLAPFILPIIFIFVIFYMITNQIKGSSMQAFS
                     FGQSRARMIDPNDEKQKVLFKDVAGVKEAKEELKEIVDFLKSPKKFIDIGAKIPKGVL
                     LMGAPGTGKTLLARAVAGEANVPFLYLSGSEFVEMFVGVGASRVRDLFKLAKKAAPAI
                     IFIDEIDAVARHRGTGMGGGNDEREQTLNQILVEMDGFEPNEKVIIMAATNRPDVLDQ
                     ALLRPGRFDRRVTLDLPDVKDREEIMTIHAKGKPFAEDVNLKVIAERTPGFSGADLAN
                     VVNEASILAAREDRKTVGQYDLIRSVEKVMLGPERKSHILSDKEKEITSYHEMGHAII
                     ASVLEHSDPVHKVSIISRGRAAGYTLKLPFEEKKLHSKKEFLDDIAVSLGGYAAEKLV
                     FGDMTTGASNDIAVSTALARDMVTRFGMSETVGPVDFGATTHEGMGVISHSHSEEFAA
                     NMDREVSKIMNESLARAEKALAEHKKALDNMSIELKKIETLERKDFENLLILNGIKPK
                     VDDSIEATAGQVIAEA"
     gene            6663..6896
                     /locus_tag="P5056_00740"
     CDS             6663..6896
                     /locus_tag="P5056_00740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62287.1"
                     /translation="MEKIIGTKELRNNLDDYIAKIQKGASFLVVRRSKPVFKISSPVA
                     EDERWEEVVDFTKLKKGGLDIDDLLSRLEKCNG"
     gene            6889..7128
                     /locus_tag="P5056_00745"
     CDS             6889..7128
                     /locus_tag="P5056_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004495675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HRY62288.1"
                     /translation="MDKIDKFLRKIPGNVRRDIFKILGLIKEGKIDNLDIKKLKGKND
                     IFRVRKGDIRIIYRVVDDKIYILAIEKKGDNTYNL"
     gene            7269..7934
                     /locus_tag="P5056_00750"
     CDS             7269..7934
                     /locus_tag="P5056_00750"
                     /inference="COORDINATES: protein motif:HMM:NF024776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRY62289.1"
                     /translation="MARKIEKQKALELREQGKSYSEIKSILCVNKSTLSGWLKDLPLS
                     AEKIAELRDNNPKRIENFRKTMAAKKEARLSASFEKASRDVGKLSDRDIFIGGLFLYW
                     GEGSKRDKGGISFTNTNPDMIKYFVRWLVLMGFRRDQLKVNLHLYSDMNAGEAINYWS
                     KELKIKRAQFRRPYIKKTESKNIKYKSGFGHGTCSVILEKQEMNDYILSALKYLAQGS
                     DKM"
     gene            7979..8051
                     /locus_tag="P5056_00755"
     tRNA            7979..8051
                     /locus_tag="P5056_00755"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8012..8014,aa:Ile,seq:tat)
     gene            8422..10761
                     /locus_tag="P5056_00760"
     CDS             8422..10761
                     /locus_tag="P5056_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011073372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
                     /protein_id="HRY62290.1"
                     /translation="MRARKINYIEAIKKHVRAANYITAAQIYLQDNFLLSEPLKSEHI
                     KPRLLGHWGTCPGINFVYANLNHLIKQTGANIMFLLGPGHGFPALQANLFIEGTLSKY
                     FPEATRDEKGLAYIAKQFSWPYGFPSHSNPTTPGVILEGGELGYALSTAYGAVLDNPD
                     LIAACLVGDGEAETGPTATAWHLNKLVDPATNGVVLPILHLNGYKISGPTLFGRMSNK
                     QLLSLFRGYGYRPIIVEGEDDHVYKKMADALAKAYRMIGTLKKKAAKGDKFDSLPFPM
                     IILRTPKGWTGIKELHGEKMEGNALSHQVVGHNAKTDKTELSAIEEWMRSYNFSELFD
                     AQKGFVSELDNVTPDAEHRMGDNKNAFPARVMKNLVLPDIKKIDDDKEKFGEEGKGSM
                     ARAGQFMKEVFLLNKENNNFRVFSPDETYSNRIDEVFKAVKRAFLLPNKPWDKDYGRD
                     GKVVEMLSEHTLQGLMQGYVLTGRHGFFVSYEAFIQIVGSMADQYAKFLKVARSVPWR
                     GDVPSFNYILTSTGWRQDHNGFSHQNPGFISEMLQKHGSFVRAYFPPDESSMLAVMKE
                     CAGSKNQINIIVAGKTPEPQWLSLHDAEKSVKEGMMIWEHVSEENPDIVFAGIGDYLT
                     KETIAAIKLLKNVAPEIKSRFVNILELSTFGFGNADRKMSADDFEKYFTEDKPIIFNF
                     HGYPEVIQSMLFHHGDSSRFSVHGYIENGSTTTPFDMHIRNKTSRYHLAIDALQKMSA
                     AGVIDNKKAESIIKDINKNIEDHREYILKNGIDPVGKFE"
     assembly_gap    10921..11020
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11021..12268
                     /locus_tag="P5056_00765"
     CDS             <11021..12268
                     /locus_tag="P5056_00765"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006083 - acetate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate/propionate family kinase"
                     /protein_id="HRY62291.1"
                     /translation="YGYEYGRNRLVEKLPTGQVLIFWNKMEKKILIVNPGSASKKYSF
                     YVDGKEVLRAHFESENGGFVVSWGSDKVNEKKNITEFDFKKSAAIVLARLKEAGIMAD
                     AGEIHSVGFRTVAAGDYFLETRFIDEEFLKQLAGAEDRAPLHVSALHLELREFAIILP
                     ETKKYAVSDSTFYKERPDYSKNYGLPYSLAKELQMFRFGYHGISGASIARRAPLVLGN
                     LPSRVVVCHLGSGISIIGIKDGKGIDASMGFTPLEGTMMSTRVGNIDPGALIHLIKRK
                     GFSPDELNTFLNKKCGLLGLSEISDDMRELLKKEAEGDLSAKRAIDTFVYQIKKQVGS
                     LIAVLGGIDSLIFTATMGERSNIIRERVCAGLEGLGIKINKAKNDEAIGTDGVISEEG
                     SAVLVAVIKTDEMREMFEDVNRF"
     gene            12471..12743
                     /locus_tag="P5056_00770"
     CDS             12471..12743
                     /locus_tag="P5056_00770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015393859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5652 family protein"
                     /protein_id="HRY62292.1"
                     /translation="MRYIDYGPGFGMMGAGLGLGFGVGTLILIAWTLYWKGKALWRAA
                     RLGHLEWFIALLIINTAGLLEIAYLYYFTKSEKAEMEVVSDKEKKD"
     gene            12793..13314
                     /locus_tag="P5056_00775"
     CDS             12793..13314
                     /locus_tag="P5056_00775"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HRY62293.1"
                     /translation="MLRSHRDIAEVILGVLIFCAVFVIAEKSGLREKITGNEALLTRG
                     VEGAHLYARESFYDFGRISMRDGEKKISFEIHNNGKEDVSIKKIYTTCQCLEVSLIRG
                     SEKIGPFSVRDYLYPGNGQMIIKKGEKVTAEVVYTPSLQGPFGVGLSEPFAVIEDGRG
                     EKIDLRISALVFP"
     gene            13443..14246
                     /locus_tag="P5056_00780"
     CDS             13443..14246
                     /locus_tag="P5056_00780"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HRY62294.1"
                     /translation="MFDENGKVSRRFINICVVSLLAVFAVALFYISGLVPEFLRGAMA
                     DKRFMLPMVIVASVVDSINPCAFSVLILTIAFLMSLNKDRATILRMGGVYILGIFLVY
                     ILIGLGIIEALQFFGVPNFMAKVGASILVVFGLLNIINDFFPSFPIKVKIPQFAHKKI
                     AYIMEKASLPSSFFLGIMVGLYEFPCTGGPYLMILGLVHDNETFWSGFLYLVLYNLIF
                     VLPLVVILLVSGSKAVLAKVSEWKGAESKNMRFWGGAITIVLGLIIFAL"
     gene            14262..14759
                     /locus_tag="P5056_00785"
     CDS             14262..14759
                     /locus_tag="P5056_00785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62295.1"
                     /translation="MNDKTKMEGIIKKVEEMRKSGTVDLSLDEDLSIAIMNLISLEEH
                     FFFTGEKTKDSSYFNMLAEVRETRKELLQKLMSKNDGESWCISKHLLAASMRLMEVGT
                     KLQGSGKKDEAKEFFDRSYRMYSLFWALRLKIIDTAGLKEVAAGDKPWTVDDIVTKLV
                     NCCDE"
     gene            14818..15198
                     /locus_tag="P5056_00790"
     CDS             14818..15198
                     /locus_tag="P5056_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62296.1"
                     /translation="MNKLNQLLWVLVFVVVVMSISGIAFFFVNNSAESSYTNLDEFAQ
                     CLTNKGAAMYGAAWCSHCAAQKERFGSSFKYATYVECPDNINACTDKGVLSYPTWILG
                     DGTKLVGEQNLEKLSELTSCELKK"
     gene            complement(15245..15958)
                     /locus_tag="P5056_00795"
     CDS             complement(15245..15958)
                     /locus_tag="P5056_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62297.1"
                     /translation="MQFYSDKTTAWNYLFNIGDGAVFILAGALPLIHGAKIKITGTPK
                     TGIVVGALSLILYGVGLMVWAFYNFYYTNAIPFPSLADVFFIIYYPFVFWAIFIYLRM
                     FLLTIKTSIYVESVVALTLLSLFVFSFLDLSYLADSSMLQTALTVFYPFASAFAISTA
                     LIIARVSGGRMSLASTFLFAGLVSRGMADFFFSFRTINGTYWNGDFSDILFALSAYLM
                     GMAWLVIISQLIPQKPETN"
     gene            16099..18105
                     /locus_tag="P5056_00800"
     CDS             16099..18105
                     /locus_tag="P5056_00800"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRY62298.1"
                     /translation="MPVLGKFFSAPKDTAEDIKKIDVSVGKVFDSARNFINREDLALG
                     CSSMLEKANSLADKEREGQLVRAYFCFEEFITENKPLVVKQEINKIDLRKEISKKAPI
                     DELPLKLKIPFLDKRNQLLSVFGLATNSLVKYLKSNIGETNFNTFLRASVAGSVLSGR
                     VSLVGDYLVFEQISGSSDLLINDKELEYSLKNLFDSLAKEVSAGFGRSMSEALVKSSL
                     DSFSSVLPLEFIRIFLDIAPLDFLTEEKASILERGELQKKFTEVVKEERTRSHEAEEL
                     SRQLQQTVSELNANKAAVLNLLEDIGAEKNKIEKEVQLRTEELKNERSVLRSTIDSLP
                     VACFLISSELVLLENNSLLARVFSADKIDSFYDMENRFNGFFSIRERCLAVLENLKPQ
                     VFPNISYGNKFFKIYLQPVVSVDTGSKIGLLGIIEDITEQKILDRAKDEFFSIASHEL
                     RTPLTAIMGNTSMIMEFFEAQLPNNEVKQMVEDIHEASTRLLKIVGDFLDVSRLEQGK
                     IIFKSTKFDLGEVVKELARDFDSAATLKGLKLEVGEISDGVSVYADESKVRQIMSNLI
                     NNAISYTQKGGVSLYLKKDEPGFVSCYIKDTGVGISKENQMLLFRKFQQAQEKILTRD
                     ASRSTGLGLYISKLLATAMGGRVELVESELGKGSTFRVVLPIKV"
     gene            18147..18533
                     /locus_tag="P5056_00805"
     CDS             18147..18533
                     /locus_tag="P5056_00805"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY62299.1"
                     /translation="MAKILIIEDDPLMLRMYERVFKLNKYEVEMAFDGEDGIKKLRSA
                     EIKPDIVLLDIMMPKMSGFDALKIIKADDSIKDIPVIILTNLAGKDEAEKGLKLGAIS
                     YLVKSEHEPKEIISIIKENLEKAGKK"
     gene            18634..20214
                     /locus_tag="P5056_00810"
     CDS             18634..20214
                     /locus_tag="P5056_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HRY62300.1"
                     /translation="MTLKRFFPVFVFLLFIFAGNTFAATTPETGNFTVTVSVSKKSSD
                     GGGVMPNNPNLNPNVIPVVPPEENIITGEISCTTEEGGVCPGFLPPEEITPGQQITEI
                     AEEVIPVAPIVPETVIDLTEPPAEVISDVVDTINNIVENTVDNVTQFSNDVLDNITEF
                     SNNIAEGAAEYSVAVLDAVQNFVKDPVAGAVNISVKITEATRDFVESPTGKVITGVAK
                     PLGVAAGATFVAVQTVVGTGMATVTSLSDIYLMLWKLVGILVGSSKRRGRPWGTVYDS
                     VTKRPLDPAYVVASRITGEEVADAITDLDGRYGFFIPAGTYKLRADKTNYSFPSKKME
                     GKNSDEIYNNIYHGEELTAGEGEVLVENIPLDPIGFDWNEFAKNKQNLFRIYSKRERF
                     WSRIFDGIYFAGLLSAFIATLFDPRIINFIFIAIYALFIIHGMRKKGRKKAVSVKSAA
                     SSLPIPFAIIKVFFADMNNLTKVAVADHLGRFYFLVGPGRYYITVDEKQADGTYKTIY
                     KSATMDLKHGILDKDILV"
BASE COUNT         5963 a         3610 c         5012 g         5559 t
ORIGIN      
        1 gaagagttaa gagctaaaat taataagtgc caaattagtt aaaatatatg aaaaaagtat
       61 ttatttattt tctcggtttg gtttcattat ttttttgttt taatttttct cttgctcaaa
      121 cagcgaccaa ggtagtaaag gtgggcgctt ttaataatta tccggttatt tttaaaaata
      181 cgagcggttc tatagaaggt ttgtatgtag atcttttaga agaaataggt aaacgggaga
      241 atatcaagtt tgattatgtt tttggcacgt ggaacgatgg gcttgagcga ataaagtccg
      301 gtgaagttga ccttctaacg agcgtggcat acacggaaga tcgggccttg attatggagt
      361 atacgaagaa ccctcttatg accgtttggg gggacgttta tgcccctaaa aattctgata
      421 tctccggtat tctggagtta tcaggtaaaa aaataggagt tttgattggc gatataaatt
      481 ataaaaattt tcaagattta ttaagtcggt ttggcttaga aagtgagttt gttgaatttt
      541 ctagttatga tgatgttttc gccgccgttg cagacggtga agtggatgtc ggagttgcag
      601 gtataacatt tggcgccacg aatgagagta agtatggttt gaaatcgacc ggaattgttt
      661 ttggtccgtt aaatttgtat tttacaaccg caaaagataa aaatcctgaa ctaatactca
      721 cgctagataa gtatctatct tcgtggaaga agcaggataa ctcttttttg gatcaaacta
      781 aacaaaaatg gttttatgga ggtcttggaa cagtaatagt attgccggac tggataaagc
      841 aatcgctctt tgcactttta atcttactat ctatttcttt tgcgttcgtt ttcttgttaa
      901 gaaaacaggt tcgcgccgca actgaaaaga taagggctca gacaaaagat cttgaagaaa
      961 gcgaattaaa ataccgctca atatataata attttatgga tgtttattac cgagcagata
     1021 tgaacggtat tattatcgag gcctcgccgt cttgtgcaga tgttacgggg tggagccaga
     1081 ctgatctcgt tggaaggccg gtgtttgata ttagtttcaa tccggaagat caaattgtct
     1141 ttagggataa acttttgaga gacgggtatg ttaatgatta tgagataaag ttattaaaac
     1201 gtggcggttc tccgtttgtg ggttcgatat ctagccatat ggttcaggac gagaagggga
     1261 atcctaaata tatagagggt gttgtaagag atattacaca gagaaagaaa attgaggagg
     1321 aactgcgcag aagcgttgaa gaattggaaa aaatgaataa gtttatggtt ggcagggagg
     1381 tgaagatgac agaaatgaaa aaagagatag acgagcttcg ggcgagaatg gggaagtagg
     1441 gggaggggaa tcggtcacaa gtaaatttcc tgacggaaat gaatggaggg ggaatcggcc
     1501 acgagttact aagtctgctc atccgccaaa ggcggattgc gcggactaag tactctcggc
     1561 cctacctcag ctccctttct tgcagaaagg ggcccgcttc cggttttcga ttcccgccat
     1621 ctccacaaac acaaaacaac accaaggcga cgccttggtg ttgtgtgttt gtggagatgg
     1681 ggggaatcga acccccgtgc aaaggatttc tctaaactac cgctagaaga tatagtctaa
     1741 ttttattttg taatgcctag ttagcgttac atttcggaaa cggcctgatc gtaattagac
     1801 aacagcaagt cattcctatt tccttaagtt cgagaacttg gcggaaaaac aaattctcta
     1861 tcccgatttt tattacacct ccccgaagtt atcggaagtc attcgtagag atgttacgca
     1921 aaagcgtaat tgagtgttgc gaaaggggat acgatcctct ttgctacatc tgacaagttt
     1981 gcacttatcg ttcccggggt tgttttacga gcatcccgag ttcctcgatc ttcagcagtt
     2041 cagatacgac caatgtctag gccgatcatc cccaaaattt tgcgaagcaa aatttagtta
     2101 taaagtggaa agttataaag tttaaagttt gtgactttat aacttgataa cattaaaact
     2161 tttgaactaa ttttaccttc cttccttaat tattctttca atgttctttg ccgtttcgcg
     2221 cttctttatc gtttcgcgtt tgtcgaattt cttctttcct ttggcgatag cgatatctaa
     2281 ctttaatttg cgtcctttaa tataaaccga taacgggatt attgtcaagc ccttctggct
     2341 agtattgccg ccaagttgat tcagctcctt ttttgtcagc aaaaggcgcc tgtttctaag
     2401 gcggtcataa tctttggggg cgtttttggg ctggtgggcc ggaatatcgg catttactag
     2461 gtatgcttct ccacctctta taatcacata ggcggcatca ataaccccgt ggccgtcttt
     2521 tagcgacttg acctcggtcc cgagaagctc tatgccggcc gacattttct ccaaaacttc
     2581 atattttagg ccgtattttc ggtttattat gagcgacata tgtgtagtgt aacagaaaac
     2641 gggggagatt ataaggggtt tacttaggaa gcaccccgcc gatggcgggg caagcttgac
     2701 aacatgcggg ccttgtgata acctcattgc cagtgacttt gagagtggcc ctgccctgta
     2761 gtgagctttg ctctactacg ggggttgaca agattattgt cttgtgctat acttaataaa
     2821 gttaaataag ttcagttcct tacaactaaa tagaagttaa tcaataaata catcgaaggg
     2881 aggccccatg ccgcattagc tagcgtaagc tgacgaacag cagagagcgg ggtttcgtca
     2941 ccccaggttc tggaaggagt tccaaaacgt gaggcgagtc ctattcgggc aggaagctgg
     3001 aacctgcctt accaaaaccg gccgggctgc gacggtagtc gctgtctgaa ccgcaagcac
     3061 taaatcccac tgtgggaagg tgcatgagaa aaacttctgt taccctgggt tgcattccga
     3121 tgctgtataa catcgcgcat cgaacgaacg cgcgccggat gcaagccaac tgcggcctgg
     3181 gttctcccgg ttctacatac ccggcagaaa ctcggtcggc acaaaagggg tacatgaagt
     3241 ttcactagcg ctaagcccag acaggcgaaa gcgctccatc cccgagcctg gggaaaatta
     3301 ggaggacacc atgttcttcc cttcggtcag aattatccag agcgggcagt gctggctgcc
     3361 ttggtgaaat aaaccagtag gttgatgaag tcggtcccgc cccctaggtc gcgctccttg
     3421 cgcgatggga cggcccgaat agccaacctg acaccctgct tgcgggggta acgaaacaga
     3481 cgagaggcgg gggtacctgc ggggcgcgcc ttgatcgtaa cgacggcgca cccaatcggc
     3541 gaaagccgcc ctaacgccct gtttgtggag ggaatgccct cactcgaaat accgcagtga
     3601 aagaagaggg tccttgataa ctacagcaag acggcatcgg ctgttgtccc cgaaagggaa
     3661 aaggatgccg cgaggagacg gcgtgaaagg ggagaccatg gtcttcgtga tcaagatctt
     3721 cactcatgcg gagtaagtcc gcattcaaca ccgaacgaag caccgcgaac ttcacgataa
     3781 cgggcgagac agtaccgccc ctagacagcc aaaccgcgaa acgggccgca gcagtacggt
     3841 agaagttctc caggaaactt gttgctaaat ccatgtcgac ctgcgggtgg cgtggtgatg
     3901 cggcagacct ccgggtttga atgctcccgt tacttgctgc gggccccgag atttcgcgga
     3961 tacatggcag gatgaactcg agtatagcga tttgtggggc tcgttgagag caaaccccag
     4021 gtgtcccagg gtgaattggg cacaggtaaa gccggagcct gaacgttggt taaacttaag
     4081 tcctaatccg gaagtttggc aaaggcccag agtttcgggt acgccagacg attaacttct
     4141 aataagcgag atcttggcct gccgttctct ggatctaggc tggctgagcc cgcttttgca
     4201 gccgcttcgc ggtgtcggga cgcttacggc cccaaatgag gaagacgctt cggtgccttc
     4261 accatgggac cgcccccgca ccacggaacg gccacatttt aacgccccga cactgagttg
     4321 tgtcggcggc gctccccaaa cagcggcgtg tctcagggct cagccttgat gatagacacc
     4381 cgctccgcag acatcgctgt tgtagtttac tgatgcggtg gtgtgcctcc agtggaggac
     4441 catcatccct cagcgatgac gttacgcggc ccccctgact tcggtcggag ggggcttttt
     4501 ttatggttta ttttctgtat aaccccagtg aaataggagc cctatagatt tatggggtat
     4561 aatagtttaa tatgagcatt gaagttttcg ctgtaatata aggttaattt cacggggtaa
     4621 ataacatgag caagaaaaat aataataaaa gcggtaagaa tgacccatgg aaggcgatgt
     4681 cacaatttgg caggagcatc ctcggtttca ttcttatttt cgtattaatt atcggccttt
     4741 attcggccta tgttgaggag tcaaaaaaga ccgaggatat atctatttcg gttctttcat
     4801 ccgacatcaa ggcgggactc gtgaaaaaaa taaccgttga gggagattat ctttatgccg
     4861 attataacga tgaaaaacag aagatctccc agaaagagct tgagacatct ctcacagaga
     4921 cccttgttaa ttatggtgta acaggtgagg agcttgcaaa agtaaacgtt gaggtcaagc
     4981 agtcatccgg tgttatgttt tggatcataa accttgctcc gttcattctg ccaataattt
     5041 tcatcttcgt gattttctat atgatcacga accagataaa aggttcgtct atgcaggcgt
     5101 tctctttcgg ccagtctcgc gcgagaatga tcgacccgaa tgacgagaag cagaaagttc
     5161 ttttcaagga tgtggccggt gtgaaagagg cgaaagagga attgaaagag atcgttgatt
     5221 ttttgaagag cccgaaaaaa tttattgata ttggcgcaaa gattccgaaa ggagttctcc
     5281 ttatgggtgc gccgggtaca ggaaaaactt tgctcgcaag agctgtggca ggggaggcga
     5341 atgtgccgtt cctatatcta tccggttcgg aattcgttga aatgttcgtc ggcgtcggtg
     5401 cgagccgtgt tagagatctt tttaaacttg cgaagaaggc agctcctgcg attatcttta
     5461 ttgatgagat agacgccgtc gctagacacc gaggcaccgg aatgggtggt ggaaatgatg
     5521 aacgtgagca gaccttgaac cagatactcg ttgaaatgga cggatttgag ccgaatgaga
     5581 aggtgataat tatggccgct acaaataggc cagatgtgtt agatcaggcg cttttaagac
     5641 caggccgttt tgataggcga gtaacgcttg accttcctga cgtaaaagac agagaagaga
     5701 taatgacgat tcatgcgaaa ggaaagccat tcgcggagga tgtaaatttg aaagttattg
     5761 ccgagagaac gccgggattt tcgggagccg atcttgctaa cgttgtgaac gaggcatcta
     5821 tcttggccgc aagagaggac agaaagaccg tcgggcagta tgacctgatt cgctctgttg
     5881 aaaaggtaat gctcggcccg gaaagaaaga gccacatttt gtcggacaaa gagaaagaga
     5941 taacttcata tcatgagatg gggcacgcga taatcgcttc cgtattggag cactctgacc
     6001 ccgttcacaa ggtttcaatc atttcacgag gccgtgccgc tgggtatacg ctcaagcttc
     6061 cgttcgaaga aaagaaacta cactccaaaa aagaattcct ggatgatatt gccgtttcgc
     6121 ttggaggcta tgctgcagag aagctcgtct tcggtgatat gacaacgggc gcttctaatg
     6181 atattgccgt ttcgaccgcg cttgcgagag acatggtcac tcgatttgga atgtcggaga
     6241 ctgtggggcc ggtagacttc ggcgcgacaa ctcacgaggg gatgggagtg ataagccact
     6301 cacactcgga ggaatttgcc gccaacatgg acagagaagt ttcaaaaata atgaatgagt
     6361 ctctcgcgcg cgccgagaaa gcgctcgccg aacacaaaaa ggcgcttgat aatatgtcta
     6421 tagagctcaa gaaaatagaa acgctcgaaa gaaaggattt tgagaatttg cttatcctaa
     6481 atggcatcaa gccgaaggta gatgatagta ttgaggcgac agcagggcag gtgattgcag
     6541 aggcatagat tgggcaccat attttagact tacaaattag ccacttggtt gccgggtttc
     6601 caagtggctt tgtttttaaa tatagggttg actttaagat taacttaaga taaacttaaa
     6661 aaatggaaaa aataatagga acaaaagaat taaggaacaa tctcgacgat tatatcgcca
     6721 agatacaaaa aggggcttct tttctggttg tcaggaggtc taagcctgtt tttaagatat
     6781 ctagcccggt tgccgaagat gaaagatggg aagaggttgt tgattttaca aaattaaaaa
     6841 agggaggact ggatattgac gacttactct caaggcttga aaaatgtaat ggataagata
     6901 gataagtttt tgcggaagat cccgggaaat gtgagacgcg atatctttaa aatattgggg
     6961 ttgataaagg agggtaagat agataattta gatatcaaaa aattgaaagg gaagaatgat
     7021 atttttcggg taagaaaagg agatatacga ataatttata gggtggtgga tgataagatt
     7081 tatattcttg cgatagagaa gaagggagat aatacatata atttgtagat tagaaagtag
     7141 tgggtggaga gtagaaagtg gttagataaa ttgaaaaaga ccccttaagc ggggcctttt
     7201 tgatctactg agtctttgat aaagctctta tctttgctat aataaagata atataaaggt
     7261 tcgattttat ggcaagaaaa atagaaaaac aaaaagcgct tgaattaagg gaacagggca
     7321 aaagctatag tgaaataaaa agtattttat gtgtgaacaa gagcactctt agcgggtggc
     7381 tgaaagatct tccattatct gcggagaaaa tagccgaact tcgcgacaat aatccaaaaa
     7441 gaatagaaaa tttccgaaaa acgatggccg ctaaaaagga ggcgcgcctg tcggctagtt
     7501 ttgaaaaagc atcccgagat gtaggtaaat taagcgacag agatattttt atcggtggcc
     7561 tgtttcttta ttggggcgaa gggtctaaga gagataaagg cggaatttct tttacaaata
     7621 cgaatcctga tatgatcaaa tattttgtaa ggtggttggt gcttatggga tttaggcgcg
     7681 accagctgaa agttaatctg cacttatatt ctgatatgaa cgcgggggag gcgataaatt
     7741 attggagcaa agaattaaaa ataaaaaggg cgcagttccg tcggccatat attaagaaaa
     7801 cggagtcgaa gaatattaaa tataaaagcg gtttcggtca cggcacttgt agcgtgatat
     7861 tagaaaagca ggaaatgaac gattatatac tttcagcact taaatatttg gcgcaaggtt
     7921 cggataaaat gtgatattta cttattggcc tttttgattt atattagggg ttacaaatgc
     7981 atgtatagct caattggtag agcaattgtc ttatacacaa tcggttccag gttcgagtcc
     8041 tggtacatgc acaacaaacc gcctgaaggc ggtttttgtg tatgtaagca agcaaactgc
     8101 tttgcttgcg tccaggactc gaaaagcttc tcccatattt ttgaggattt ttaatccgaa
     8161 aaaatgggaa agctatactg tcctcgtaga ggaagagtcc tcctagtaca tgcacccgaa
     8221 gactcgcttc gctcgaacgg gcgggcaaca aaccgccttc gggcggtttt attttgcgtt
     8281 ttttggtata ctggttttgt taaatagata attaaattca tggccaaccc accaaaattt
     8341 ttagttctta cgttattggt gttttaaaat gaaaaaagga aaaattatct aataatctat
     8401 taataacttt tgaaaaaatt tatgcgggca agaaagataa attacataga agcaatcaaa
     8461 aagcacgtgc gggcggcaaa ttatataacc gcggcgcaaa tatatttgca agataatttt
     8521 ttgttaagcg agcctcttaa gtcggaacac ataaaaccga gacttttggg tcactgggga
     8581 acctgtccgg gaataaattt tgtttatgcg aacttgaatc atctcattaa gcagaccggc
     8641 gcgaatataa tgtttctgct aggtccgggc cacggatttc cggcccttca ggcgaatctt
     8701 tttatagaag gaactctctc aaaatatttt ccagaagcga cacgggacga aaaagggctc
     8761 gcatatattg cgaaacaatt cagctggccg tatggctttc caagccacag caacccgaca
     8821 accccgggcg taatacttga gggcggtgag cttggttatg ctctttcgac cgcttacggt
     8881 gcagtccttg ataatccaga tcttattgcc gcgtgtctcg tgggagacgg cgaggcggag
     8941 accggcccga cagcgaccgc atggcacctt aataagcttg ttgatcctgc gacgaacgga
     9001 gttgtgctcc cgatactgca tttgaatgga tacaaaattt ccgggccgac tctttttggc
     9061 agaatgagca acaaacagct tctttcgtta ttcagaggtt acggttaccg gccgataatt
     9121 gtggaggggg aagacgacca tgtttataaa aagatggctg atgcgctcgc gaaggcatac
     9181 aggatgattg ggacccttaa gaaaaaagca gcgaaggggg acaaattcga ttcgcttcct
     9241 ttcccgatga taattttaag gacaccgaaa gggtggacag gcattaagga acttcatgga
     9301 gagaaaatgg agggaaacgc tctctcacac caggttgtcg gacataatgc caagacagac
     9361 aagacagagc tttctgcgat agaggagtgg atgaggtcat ataatttctc tgagcttttt
     9421 gatgcgcaaa aaggttttgt aagcgagcta gataatgtca ctccggatgc ggagcacaga
     9481 atgggagaca acaagaacgc ttttcccgcc cgcgtaatga aaaatcttgt tttgcctgat
     9541 atcaaaaaaa tagacgatga taaagaaaag tttggtgaag agggtaaggg gagcatggct
     9601 cgcgcggggc agtttatgaa agaggttttt ctgctcaata aggagaacaa caacttcaga
     9661 gtcttttcgc cggatgaaac atactcaaac aggatagatg aggtctttaa ggccgttaaa
     9721 agggcgttct tattgcccaa taagccttgg gacaaggatt atggccgaga tggtaaggta
     9781 gtcgagatgc tctcagaaca cacgttgcag gggcttatgc agggctatgt actcacgggg
     9841 cgccatggtt tttttgtatc atatgaggcg ttcatccaga tagtgggaag catggcggat
     9901 caatacgcaa agttcttgaa agtcgcgagg agtgtgcctt ggagaggcga tgttccttcg
     9961 ttcaattaca ttttgacatc tacaggatgg cgtcaggacc acaacggctt ttctcaccag
    10021 aaccccggat ttattagcga gatgcttcag aagcacggtt cattcgtgcg agcttatttc
    10081 ccgccggacg aaagcagtat gctcgcagtt atgaaagaat gtgccggttc aaaaaatcag
    10141 atcaatataa ttgttgcggg taagacgccg gagccgcagt ggctatctct acacgatgcc
    10201 gagaaatctg tcaaagaggg aatgatgata tgggaacatg tcagtgaaga aaatcccgac
    10261 attgtttttg ccggaatcgg cgattatctg acaaaggaga ctatcgccgc gataaaatta
    10321 ttaaagaatg tcgctccgga aataaagagc agatttgtga atattttgga actctcaact
    10381 ttcggtttcg ggaacgccga tagaaagatg tccgctgatg atttcgaaaa atatttcacg
    10441 gaagataagc cgattatttt caattttcac ggataccctg aagttattca gtcgatgctg
    10501 tttcaccatg gagattcgtc tcgattttct gttcacggat atatcgaaaa cgggtcaacg
    10561 acgacgccgt ttgatatgca cataaggaat aaaacgagta gatatcattt ggctattgat
    10621 gcacttcaga aaatgtcggc ggcgggtgta attgataata aaaaggccga aagcattatc
    10681 aaagacataa ataagaatat agaggatcat cgtgagtata tattaaaaaa cggcatcgac
    10741 cctgtaggaa agttcgaata gattattatc aatttttaac tgtatcgctc gggcccgcct
    10801 tacggctacg aatacggtcg taataggtta gttgagaaac ttcctacagg gcaggccccg
    10861 atttctagga aagttcgaat agattattat caatttttaa ctgtatcgct cgggcccgcc
    10921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tacggctacg aatacggtcg
    11041 taataggtta gttgagaaac ttcctacagg gcaggtcctg attttttgga ataaaatgga
    11101 aaagaaaatt ttaatcgtaa acccgggtag tgcatcaaaa aaatattctt tttatgttga
    11161 tggaaaagag gttttgcgtg ctcattttga atctgagaac ggcggattcg ttgtaagttg
    11221 ggggtcggat aaggttaatg aaaaaaagaa tataaccgaa tttgatttta aaaagagcgc
    11281 ggcgatagtt ctcgccagat taaaggaggc gggaattatg gcggatgcgg gcgagataca
    11341 ttctgtcgga tttaggaccg ttgcggccgg agattatttt ttggaaactc gttttattga
    11401 tgaagaattt ttaaaacagc ttgccggagc tgaagacagg gcgccgctac atgtatcggc
    11461 acttcattta gaattgagag agtttgccat aatattaccg gagaccaaaa aatatgccgt
    11521 atcggacagc actttttata aagagaggcc ggactattca aaaaattacg gacttcctta
    11581 ttcattggcc aaggaattgc agatgtttcg atttggttat cacggcattt ccggggcctc
    11641 gattgcgaga agggcgcctc tagttttagg aaatttgccg agcagggtgg tcgtgtgtca
    11701 cttgggaagc ggtataagta ttatcggaat aaaagacggg aaggggatag acgcttcaat
    11761 gggttttact ccgcttgaag ggacaatgat gagcacgaga gtcggaaata ttgatcccgg
    11821 ggcgctcata cacttgataa agagaaaagg attttctccc gacgagctga atacttttct
    11881 taataagaaa tgcggacttt tgggattgtc tgaaatatca gacgacatgc gcgagctctt
    11941 aaagaaggag gccgagggtg acttaagcgc aaaaagagca attgatactt ttgtttatca
    12001 gataaaaaag caggtgggtt cactcattgc cgtgcttggc ggcattgaca gcctcatctt
    12061 caccgccacg atgggagaga gatcaaatat aataagagaa agggtgtgcg ccggattgga
    12121 agggctgggt ataaaaataa ataaagcaaa gaacgatgaa gcgataggaa cggatggtgt
    12181 gataagcgaa gaaggttccg cggttttggt tgcggtcatc aagacggacg aaatgagaga
    12241 gatgtttgag gacgtgaata gattttagat tttttattat tttttatata atctaattga
    12301 ggccaaagag tcgaagaaat ctgcacaaca gattgctcaa tgcattttaa agtattgata
    12361 gacacgctgg cctcactaaa aaccctgtaa atagcgggtt ttttagtttt cggctactat
    12421 ttgtttgacc gttttgttat aatacaagta attacaagat attatatttt atgagatata
    12481 tagattatgg tcccggtttc gggatgatgg gagcggggct cggtttggga tttggagtcg
    12541 gcactcttat tcttatcgca tggacacttt actggaaggg taaggcgctc tggagagccg
    12601 ctagattggg ccatttggaa tggtttatcg cgcttttaat tataaatacg gcgggattgc
    12661 ttgaaatagc ttatctttac tattttacaa aatccgaaaa agcagaaatg gaggttgttt
    12721 cggataagga gaagaaggac taaaaaccgc tcttacttat acggttggta acattctggc
    12781 ctcgcaacat taatgttaag aagccataga gatatcgccg aggttatatt gggagtcctc
    12841 atattttgcg ctgtatttgt aattgccgaa aaaagcgggc taagagaaaa aattaccggg
    12901 aacgaagcac ttttaactag aggcgtggag ggcgctcatt tgtatgcgcg cgagagtttt
    12961 tatgatttcg gaaggatctc tatgagagat ggagagaaaa aaatttcgtt cgaaatacac
    13021 aataacggga aggaagatgt ttctataaaa aagatatata caacttgtca gtgcctggaa
    13081 gtttctctta taaggggaag cgaaaagatc ggcccgttca gtgttagaga ttatttgtat
    13141 cccggaaacg ggcaaatgat cattaaaaaa ggggaaaaag tgacagctga ggtcgtgtat
    13201 acgccgtcat tacaggggcc gtttggtgtg ggtctttcag agccgttcgc ggttatagag
    13261 gacggacgcg gagaaaaaat agatctcaga atttcggctc tggtgtttcc ttaaataata
    13321 atttagaata caaaaactaa tacccgtata gaaatagaag ttttttaggt tttgtattat
    13381 gtgcgggaga aggtgcggat gatagttttg gcggtttatg agtttttaat ttcaaagggt
    13441 aaatgtttga cgaaaacggg aaagtttcca gaaggtttat aaatatttgc gtggtatctc
    13501 tcttggccgt ttttgccgta gcactttttt atattagcgg tcttgttccg gagtttttga
    13561 gaggagcaat ggcggacaaa aggtttatgt tgccgatggt tattgtggcg tcggtagtag
    13621 atagtatcaa tccgtgtgct ttctctgtcc ttatattgac catcgctttt ctaatgagcc
    13681 tcaataaaga cagggccaca attttacgaa tggggggtgt ttatattttg ggaatttttt
    13741 tggtttatat tttaataggc ctcggcatca tagaggcgct tcaatttttc ggagttccta
    13801 attttatggc caaggtcgga gcttctattc ttgttgtatt cggtctttta aatattatta
    13861 atgatttttt cccgtccttc ccgatcaagg taaagatacc gcagtttgct cacaagaaga
    13921 ttgcttatat aatggaaaaa gcgtcgttgc cttcatcctt ctttctcgga ataatggtcg
    13981 ggctatacga gtttccttgt acgggcggcc catatcttat gatattgggg ttggtgcacg
    14041 ataatgaaac attttggagc ggttttctat atcttgttct ttataacctt attttcgttt
    14101 tgccgcttgt cgtgattctt cttgtctccg gtagcaaggc ggttcttgct aaggtgtcgg
    14161 agtggaaggg cgctgaatcg aagaatatga gattctgggg aggggcgata acgattgttt
    14221 tgggtctaat aatttttgca ttataatttt ttataaattc tatgaacgat aagacaaaga
    14281 tggagggtat aataaagaag gttgaagaga tgagaaaaag cggaacagtt gatctgtctc
    14341 ttgatgaaga tctttcaata gcgataatga accttattag cctcgaggag catttcttct
    14401 ttacgggaga aaagacaaaa gattctagtt attttaatat gcttgccgag gtgagagaga
    14461 ccagaaaaga gcttcttcag aagcttatgt caaagaatga cggagaatcg tggtgcatct
    14521 caaaacatct gttggcggcc tcaatgagac tcatggaagt tgggacaaag ttacagggaa
    14581 gcgggaagaa agatgaagcc aaagagtttt ttgatcgttc ttaccgcatg tattcgttgt
    14641 tctgggcgct taggttaaaa ataatagata ccgccgggtt gaaggaggtg gctgcagggg
    14701 ataagccttg gacggtagat gatattgtta ctaagctagt aaattgctgt gatgaataat
    14761 aaaaaatgta aaatggaaat ctcaaaatgg aaaattacag gttaaaattt aaacaggatg
    14821 aataaattaa accagcttct ttgggtcctg gtgtttgtcg ttgtggttat gtctatatca
    14881 ggcattgctt tcttttttgt aaataattcc gcggagtcaa gctacacaaa tttagatgaa
    14941 ttcgctcaat gtttaacaaa taaaggcgct gcaatgtatg gagccgcttg gtgttctcat
    15001 tgtgcggcac agaaagaaag attcggaagc tctttcaaat acgctactta tgttgagtgc
    15061 ccggacaata ttaatgcgtg cacggacaaa ggggtgctga gctatccgac atggatactt
    15121 ggtgacggca caaaattagt cggcgagcag aatttggaaa agctatccga actgacttcg
    15181 tgtgaattaa agaaataaat tttatttgaa aaacacctcc cttgccggga gatgttttag
    15241 tagattaatt tgtttccggt ttttggggaa tcagttgcga gattattacc agccacgcca
    15301 ttcccattaa atacgcggat agtgcaaaca aaatatcgct aaaatcaccg ttccaatatg
    15361 ttccatttat tgttctaaaa gaaaagaaga agtcggccat tcctcgggac acgagccccg
    15421 caaagaggaa ggttgacgcc agagacattc tgccgccgct gactcttgcg attatcagcg
    15481 cggtggatat ggcaaacgca cttgcaaaag gataaaaaac ggtcaatgcc gtttgtaaca
    15541 tggagctgtc ggccaggtaa ctaaggtcaa ggaagctgaa tacaaatagc gatagaaggg
    15601 ttagcgcgac aacggattct acgtaaatac tggtttttat ggtgagcaag aacattctca
    15661 aatatataaa aatagcccaa aatacaaacg ggtaataaat aataaaaaat acgtcggcta
    15721 gcgaagggaa cggtatcgca ttagtataat aaaaattgta aaaggcccaa accataagac
    15781 ccacgccgta taatattagg gaaagagccc cgacaacaat acctgttttt ggtgttcctg
    15841 taatttttat tttagcacca tgaataagag gtagcgcccc ggcaagaata aagacagcac
    15901 catctccaat gttaaaaaga taattccacg ccgttgtttt gtctgagtaa aattgcaata
    15961 agagtcccca tataaaaaca attaagaaga gcaggcttag gatatatttg gagcgcgaga
    16021 tgcttgcaat ggtattcata tttttaaaaa tatacttgta tgttcaaatt atagtataat
    16081 tagtgtaaat agaaaaacat gccagtttta ggaaaatttt tttctgcccc gaaggatacg
    16141 gcagaagata taaaaaaaat agatgtttcc gtcggaaaag tttttgattc cgctcgaaat
    16201 tttatcaaca gagaagatct cgctctcggt tgttcatcaa tgctagagaa ggctaattct
    16261 ctcgctgata aagagcgaga aggtcagctg gtccgggcat atttttgctt tgaagaattt
    16321 attaccgaga ataaacccct tgttgttaaa caagagataa ataagataga tcttagaaag
    16381 gaaataagta aaaaagcgcc gattgacgag ttgcccctaa aattaaaaat acctttttta
    16441 gataaacgta atcagctgtt gtctgttttt ggcctggcaa ccaatagtct cgttaaatat
    16501 ctgaagagca atattggaga aaccaatttt aatacatttc ttcgtgcttc agtggccggt
    16561 agcgtgctta gcggcagggt gtctttagtc ggcgattatt tggtatttga gcaaatcagc
    16621 ggttcctctg atctactaat taacgataaa gagctggagt attccttaaa gaatttattc
    16681 gattcattgg ccaaggaagt ttccgccgga ttcggtagaa gcatgtccga ggcactggta
    16741 aaatcatccc tagattcgtt ctcctctgtt ttaccgctag aatttataag aatattttta
    16801 gatattgccc cgcttgattt tttgacggaa gaaaaagcct ctattttaga gaggggggag
    16861 ttacagaaaa aatttacaga ggttgtaaaa gaagaaagga caaggagtca tgaagcggag
    16921 gaattatcca gacagctcca gcagaccgtt tcagagctta acgcgaacaa ggcggccgta
    16981 ttgaatttgc ttgaagatat cggagccgag aaaaacaaaa ttgagaagga ggtccaatta
    17041 agaacagaag agctgaagaa tgaacgttcc gtattgcgtt caacgataga cagcctcccg
    17101 gttgcttgct tcttaattag ctccgagttg gttcttttgg aaaacaatag tttgcttgcg
    17161 cgagttttct ctgcggataa aattgacagt ttctatgata tggaaaatag gtttaacggt
    17221 tttttctcta ttagagagcg atgtctggcg gtactcgaaa atctaaaacc gcaggtcttt
    17281 ccaaatatta gttatggtaa caaatttttc aaaatttatt tacagccggt tgtgtctgtc
    17341 gatacaggaa gcaagatagg tcttttggga attatagagg atattacaga acaaaaaata
    17401 ttagataggg caaaagatga atttttttct attgcctcac atgagctaag aacccctctt
    17461 acggctatta tggggaatac ctcgatgata atggaatttt ttgaggcgca gttaccgaac
    17521 aatgaggtaa aacagatggt tgaagacatc catgaggcga gcacaagact gctgaagatc
    17581 gtgggtgatt tcttggatgt ttctcgattg gagcagggta agataatatt taaatcgacg
    17641 aagtttgatt tgggagaagt ggtaaaggaa ttagcgcgcg actttgactc ggcggccacg
    17701 ctaaaaggat tgaaattaga agttggagag atttctgacg gtgtttcagt atacgcagat
    17761 gagagcaagg tcagacagat tatgtctaac ttaataaaca atgctataag ttatacacaa
    17821 aaaggagggg tttctcttta tttaaaaaag gatgaacccg gttttgtgtc gtgttacatt
    17881 aaagacaccg gtgtaggtat ttcaaaagag aatcagatgt tgctttttag aaaattccag
    17941 caagcacagg aaaagatttt gacaagagat gcctcaagga gcacaggtct tggattatat
    18001 attagtaaat tactcgctac cgccatgggc ggcagagtgg agcttgttga aagcgagctt
    18061 ggtaagggga gcacctttag agtggtgcta ccgataaagg tatgataata tgttgcagta
    18121 gcttatatat tttataattc aaacatatgg cgaaaatttt aattatagag gatgaccctt
    18181 tgatgcttcg aatgtatgaa cgcgtcttca aactaaacaa atacgaagta gagatggcgt
    18241 ttgacggaga agacggaata aaaaaattga ggtcggcgga aattaagccc gatattgttc
    18301 ttttggatat tatgatgcca aaaatgagcg gttttgatgc tcttaaaatt attaaggccg
    18361 atgacagtat aaaagacatt ccggttatta ttttgacgaa tttggcggga aaagatgagg
    18421 cggagaaggg gcttaaactt ggagctatat cttatctcgt taaaagcgag cacgaaccaa
    18481 aagaaattat ctcaataata aaggaaaatc tggagaaagc ggggaagaaa taaacctaaa
    18541 tcagaaaata tttttaaaaa acgaccctcg tgggtcgttt ttttatccac acccgcgcag
    18601 gttttgtttt tgtttgtgtg gtattattta gaaatgaccc ttaaaagatt tttcccggtt
    18661 tttgtttttt tgctttttat tttcgccgga aatacctttg cggctacaac tccggaaacc
    18721 ggtaatttta ccgtgacagt aagtgtgtca aaaaagtcca gtgatggagg aggcgtcatg
    18781 ccgaacaatc ctaatctgaa tcctaatgtg ataccggttg ttcctcccga agagaatata
    18841 attaccggag aaatctcgtg cactacagaa gaaggaggag tttgccccgg ttttttgccg
    18901 ccggaagaga taaccccggg tcaacaaatt accgaaatag ccgaagaggt tattccggtt
    18961 gctccgattg ttcccgagac ggtaatagat ctgacagagc cgcctgccga agttataagc
    19021 gatgtggtgg atacaataaa taatattgta gaaaataccg ttgataatgt tacccagttc
    19081 tcaaatgatg ttttggataa cataacagaa ttttctaaca atatcgccga aggggcggca
    19141 gaatactctg tagcggtatt ggatgctgtg cagaatttcg taaaagatcc tgtcgcggga
    19201 gcggttaata tttctgtaaa aattacggag gccacgaggg acttcgtaga gagtcctacg
    19261 gggaaagtta tcaccggtgt tgcgaagcct cttggtgttg cggcaggtgc tacttttgta
    19321 gcggtacaga ccgttgtggg gacaggtatg gcgactgtta cgtctctgtc cgatatatat
    19381 ctcatgcttt ggaaattggt tggtatattg gtcgggtctt cgaagaggag gggcaggcct
    19441 tggggcacgg tttatgactc tgtgaccaag agacctcttg atccagcgta tgtcgtggct
    19501 tcgagaatta caggcgagga ggtcgcagat gctataactg accttgacgg tcgttacgga
    19561 ttctttatac cggcaggcac atataagctt agggcggaca agactaatta ctctttccct
    19621 tctaaaaaaa tggaaggtaa aaatagtgat gagatataca ataatattta tcacggagag
    19681 gaattaacgg ccggcgaggg agaggttttg gttgagaata ttcctcttga tcctatcggt
    19741 tttgattgga acgaatttgc caaaaacaag caaaatcttt tcagaattta ttccaaacga
    19801 gaaagatttt ggagtagaat ttttgacggt atttattttg ccggtttgtt atcggccttc
    19861 atagccactt tgttcgaccc gagaatcatt aactttattt ttatcgccat atatgcgctg
    19921 ttcatcatcc atggaatgcg aaagaagggg agaaagaagg ctgtttccgt aaagagcgcg
    19981 gcctcgtctt tgcctatccc gttcgctatt atcaaggtat tctttgccga catgaacaac
    20041 ctgaccaagg tagcggtcgc cgatcatttg gggagattct actttttggt cggtccggga
    20101 agatactata ttactgttga tgaaaagcag gctgacggaa cctacaaaac aatttacaag
    20161 tctgcgacga tggacttgaa gcacggaata ttggataagg acattttggt ataatcgttt
    20221 aaggaaacaa tgaaaaaaat attt
//
LOCUS       DAOTEX010000007        16836 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3118
            NODE_2321_length_16836_cov_4.524164, whole genome shotgun sequence.
ACCESSION   DAOTEX010000007 DAOTEX010000000
VERSION     DAOTEX010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868142
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 16836)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16836)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 883
            CDSs (total)                      :: 833
            Genes (coding)                    :: 829
            CDSs (with protein)               :: 829
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16836
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2321_length_16836_cov_4.524164"
                     /isolate="AMR_MDS_3118"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            101..1630
                     /gene="rny"
                     /locus_tag="P5056_00815"
     CDS             101..1630
                     /gene="rny"
                     /locus_tag="P5056_00815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013120159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HRY62301.1"
                     /translation="MSLKEVALISGAAGILGIGIGYFLRWLVLLAQKGSMEITIKQTM
                     LEAKTTAQKILDEAKKNSEQLIRDAKEEEKNRLSEAKKTEERLIKKDELLDKRQVDID
                     KEVENLKIKVSEIREIRSKVEEMQQKTQEEMEKIAKLSETEAKEQLFNIIERKYEDDF
                     VARIQKLERDGIDRLDKKAKDILSMSIQRLAASTASEMMTTNVPIPSDDIKGKIIGKE
                     GRNIRAFERAAGVEVIIDDAPGSITISSFDPIRRQVARMALENLILDGRIQPAKIEEV
                     VEKSRQEVNKIIKDRGDQAVYETGVLNLDPRIVAILGRLHFRTSYGQNVLQHSIEMAH
                     LAGMLAQELGADVAIAKAGALLHDIGKAVDHEVAGTHVEIGKRILEKFGADPRIIKAM
                     QSHHNEYEYETMEAVIVQTADAISGGRPGARRDSVENYLKRLGELEAIANSFEGVEKS
                     YAIQAGREIRVFVVPEKISDLEAKKMAQDIARRIEQDLKYPGEIKVNVIRETRVTEYA
                     K"
     gene            2009..2305
                     /locus_tag="P5056_00820"
                     /pseudo
     CDS             2009..2305
                     /locus_tag="P5056_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011384426.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
     gene            complement(2512..3078)
                     /locus_tag="P5056_00825"
     CDS             complement(2512..3078)
                     /locus_tag="P5056_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62302.1"
                     /translation="MKNYFIEFLFALLLLTFAAVMFLLMINRPLPAQAHDNPVYESPE
                     ISGPYSVNVAFPNLDDYIKATGTKWVDPEVLAALIRDNGRELGSKNIYLISFDYPTTS
                     GQVRRWMNNKNYYPTTLLPLFALGVDVKEIFPGYPVVALGSICHDYRGELCAPQLWMN
                     WGRKRLSIVPHDPIWPAYTRFAVVRYEN"
     gene            complement(3319..4860)
                     /locus_tag="P5056_00830"
     CDS             complement(3319..4860)
                     /locus_tag="P5056_00830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HRY62303.1"
                     /translation="MKLTIPTEVLYTTETLEKGGFEAYLVGGCVRDVLMKREPKDWDV
                     TTNATPEEIQKLFPKTFYENKYGTVGVVNEEIAKEIGSAETSKKEVFADKGGESDLRL
                     RESLRVIEVTPYRVESEYSDFRHPDEVKFSKNIEDDLHRRDFTVNAIAYNPSSGVLKD
                     IYKGQEDLKDKVLRTVGLASDRFGEDALRMLRAVRFSVQLGFTIASETSEAIIKHAAL
                     LEKISQERIRDEFSKIIMSDDPVVGVVMCQKFGLLSYIVPELEEGIGVKQNSDHIYEV
                     WEHNLRALGHAGEKKWSLEVRLAALFHDIGKPRTRGWSEEKKDFTFYGHDVVGERMVK
                     KIMERLRYPGETSDKVLRLVRNHMFFTDIDKITLSAVRRIVRNVGKENVWELMNVRAC
                     DRIGMGRPVETPYRLRKYESMIEEAMRAPVTVGMLKINGQKIMEVTKLAPGPKIGFTL
                     HALLEEVLDDPSLNTEEYLEKRAGELVKLSDEELKKLGEEGKEKKEEVEEKELSKIRK
                     KYHVK"
     gene            complement(4871..5407)
                     /locus_tag="P5056_00835"
     CDS             complement(4871..5407)
                     /locus_tag="P5056_00835"
                     /inference="COORDINATES: protein motif:HMM:NF014041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HRY62304.1"
                     /translation="MKFVIGSASKRKIEVAEKIIRQLVGGGNLFITGYPSYSGVPDTP
                     YDKQTFDGANNRARDAKAHIKDGDIFIGLESGLVERHGHIYEEAWCAILDKDEKSFYG
                     YSSGLKVPDLILKKMDELKLEHCDVMAMIEEEDGKMPSETWGNYSGGMIAREISLEES
                     LRNALIQIFSPDHSLYNK"
     gene            complement(5514..5584)
                     /locus_tag="P5056_00840"
     tRNA            complement(5514..5584)
                     /locus_tag="P5056_00840"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5550..5552),aa:Gly,seq:gcc)
     gene            5709..5780
                     /locus_tag="P5056_00845"
     tRNA            5709..5780
                     /locus_tag="P5056_00845"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5742..5744,aa:Arg,seq:tcg)
     gene            6108..6191
                     /locus_tag="P5056_00850"
     tRNA            6108..6191
                     /locus_tag="P5056_00850"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6142..6144,aa:Leu,seq:cag)
     gene            7154..7543
                     /locus_tag="P5056_00855"
     CDS             7154..7543
                     /locus_tag="P5056_00855"
                     /inference="COORDINATES: protein motif:HMM:NF014119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HRY62305.1"
                     /translation="MAIQQEIGQLGEDIAVKYLKNKGYSIIERNYWKKWGEIDIVAEK
                     ACLIHFVEVKSVSYVTPNTGVTYETPAFDPQDHMNKEKMHRLNRVIETYLADKDMENE
                     WQVDLMLVYLNKSTKTAKVNLIENITL"
     gene            7648..7718
                     /locus_tag="P5056_00860"
     tRNA            7648..7718
                     /locus_tag="P5056_00860"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7680..7682,aa:Pro,seq:tgg)
     gene            8110..8601
                     /locus_tag="P5056_00865"
     CDS             8110..8601
                     /locus_tag="P5056_00865"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRY62306.1"
                     /translation="MAILNKKDYFAQLFKLPLAAGGLFFNNKNEILLLKPTYRDGYLI
                     PGGMVEEQESPLTALKREINEEIGLQIEKGDILCIDYNPKNDSYNFDDSSIQIVFDCG
                     TLNKEQINKITLSEEHSEFKFRSIEEAVKMCNPKLAKRLPKCIEARKLNRCVYLEEGK
                     AVS"
     gene            9141..9422
                     /gene="rpsO"
                     /locus_tag="P5056_00870"
     CDS             9141..9422
                     /gene="rpsO"
                     /locus_tag="P5056_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012995240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HRY62307.1"
                     /translation="MLKKDNKAKIIKKARKHETDTGSSEVQIALLTENINKLTNHLKA
                     NKKDFHSRRGLLQMVADRRSHMKYLEKKDKKGFDALAEKLNLRKTSKID"
     gene            9441..10010
                     /locus_tag="P5056_00875"
     CDS             9441..10010
                     /locus_tag="P5056_00875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HRY62308.1"
                     /translation="MAIIKQKSQRVGVFIDTQNLYHSARNLYGAKVNFGAILADAVAG
                     RNLVRAIAYLITTESGEERGFFEALEKLGIETKTKDLQIFFGGAMKADWDVGIAVDAI
                     KLAPKLDAIVLVSGDGDYIPLVEYLKATTQVELVAFGKSCSAKLKDAVDDFIDLGGDP
                     KRYLLGQKPIKKFSKGGQSAAGAGQEKKV"
     gene            10113..12272
                     /locus_tag="P5056_00880"
     CDS             10113..12272
                     /locus_tag="P5056_00880"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: protein motif:HMM:NF008805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HRY62309.1"
                     /translation="MQRKEYSLVVGGKTVTAIFSDMVAQANGSVVMKIGETSILATAV
                     MSSNLKDGLDYLPLTVDYEERYYASGRLPGGQYVRREGKPSEEAILTGRIVDRTIRPL
                     FDQSIRNEIQVVVTTLSLDGENDPDTISVLGASLAIATSNIPWGGPVGSVRVIVKKDG
                     TLGINSGHSERKDILLDSYICGRAGTINMIETESKEVTEDKIKEVFELAMSEITKIED
                     WQKKIIAEIGKDKKVFEKKELPKEAIELFEKEIGSKMESIVFSGSGSKMSHDLLHEWL
                     DIYTEKFEDSADKNLAAGYFDDKINDLLHEEAIKNNRRADGRKMDELRKIEASVGGIS
                     DIIHGCGLFYRGETHMLSVITLGGPKDALQLDGAEVAGEKFFMHHYNFPPYSSGETGK
                     IGNTNRRMIGHGALAEKALRATLPSRETFPYTIRIVSDTMSSNGSTSQAAICASSLAL
                     MDAGVPISRPTAGIAMGLMMDSEGKYKVLTDIQGPEDHYGDMDFKVAGTRVGVAAIQL
                     DIKMDGVPVNVLVEAMMQAKKARLEILDVIEGALPAPRADLAKSAPRIFKIHINPEKI
                     GAVIGPGGKMIHHITDTTGAEINVEQDGTVFITGKAEGAAAAKKAIEELTREFKIGDT
                     MIGEVVKLADFGAFVKIGMDTDGLVHVSEIAPFRVDKVADFLKVGMKVPVIVKDKEGD
                     RISLSIKRIKPDLFKAPASASMPPRPPMTPPAATPKI"
     gene            complement(12318..12950)
                     /locus_tag="P5056_00885"
     CDS             complement(12318..12950)
                     /locus_tag="P5056_00885"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRY62310.1"
                     /translation="MKINSVIKTLVMSDFVINSAFGLSAPIFAIFLVDNIQGGNAEVA
                     GFAASVYWIAKSFLQLPIAEFLDKTDGEKDDFVVLVCGQVIASFSLFMYIFATLPWHI
                     YAIEAFLGASMAFAVPAWYGIFTRHIDSGRTSFEWSLESVFSVGVATAISSALGGIIA
                     NRYGFNVLFVGAGLWALLGALSLILLYPHLKKSDGKGETFETVEERAKII"
     gene            complement(13182..13367)
                     /locus_tag="P5056_00890"
     CDS             complement(13182..13367)
                     /locus_tag="P5056_00890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62311.1"
                     /translation="MHLNDTDREAFIAGYKQYRVREEGSVVHGVPSIVLVDEPTYKRI
                     CESRNGIWVMRTVYANL"
     gene            complement(13427..13744)
                     /locus_tag="P5056_00895"
     CDS             complement(13427..13744)
                     /locus_tag="P5056_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62312.1"
                     /translation="MKRFLFFALCFTILVSMNMFVITASHAEGDYSSFIDDDKECTAV
                     IHVSDHFASPRIHVMDLVIKKAWWVKNNPDKKIISTHFIYDKEYIIGLWIDYEIKQIG
                     DAQ"
     gene            complement(13761..14000)
                     /locus_tag="P5056_00900"
     CDS             complement(13761..14000)
                     /locus_tag="P5056_00900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62313.1"
                     /translation="MLYAVVRQRWSFSYSSDWSSLHKNERDREAFARRHVESSFGIPI
                     GAPEIVFVSEEVLNYISSGTDGIWSFSADYDVVTV"
     gene            complement(14012..14461)
                     /locus_tag="P5056_00905"
     CDS             complement(14012..14461)
                     /locus_tag="P5056_00905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62314.1"
                     /translation="MPKKKAGKNKDDNSRSNITIFADSGVRARGFGQRYLDRLAFDLL
                     RINKLSGLYKDINNQIYKTLSGVAGNRDRLATLAQIVYEATVLSTSGIEFEHEAGQAL
                     MWAARNQMIPNPGLDMTKPLMKKELREWKRWLRFQGYNLKTFRKNKK"
     gene            complement(14582..14652)
                     /locus_tag="P5056_00910"
     tRNA            complement(14582..14652)
                     /locus_tag="P5056_00910"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14618..14620),aa:Gly,seq:tcc)
     assembly_gap    14697..14706
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(15158..15307)
                     /locus_tag="P5056_00915"
     CDS             complement(15158..15307)
                     /locus_tag="P5056_00915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62315.1"
                     /translation="MYVCGTCKNEAEYPGACPLCNEKMDCTTCAGAEGADKCLACQEL
                     EGFQE"
     gene            15695..16039
                     /locus_tag="P5056_00920"
     CDS             15695..16039
                     /locus_tag="P5056_00920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62316.1"
                     /translation="MKIKEGQLSKWNNYVAEHQTGYDRTIIMFTETIANLIELNRDRF
                     WSFNISLISAIREAVVLCGIKTDDPELADAMVNEAFGLLFNYWESGWIMEEYSFRDKT
                     LYHEFRITWTLR"
     gene            complement(16108..16479)
                     /locus_tag="P5056_00925"
     CDS             complement(16108..16479)
                     /locus_tag="P5056_00925"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY62317.1"
                     /translation="MDKKVFLIEDDRFLREMLSQKLSEQKITVDVAVDGKSALEMIKT
                     IRPDLVLSDLLLPDVDGFDVLAAIQKDPNFANAPVLVLSNLDKMEDIKKAKDLGAKDF
                     MVKSNFTSNEIVAKVKEFLKI"
     gene            complement(16505..>16836)
                     /locus_tag="P5056_00930"
     CDS             complement(16505..>16836)
                     /locus_tag="P5056_00930"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRY62318.1"
                     /translation="DIIHNAIKYTKSGGSVDISTRLEAGKAIIRIRDTGIGIPENEKS
                     RVFTKFFRAKNAKDLYKNGSGLGMYISKSIITRHNGSITLESKEGEGTVVEIFLPLLQ
                     SAPRENK"
BASE COUNT         5296 a         3623 c         3387 g         4520 t
ORIGIN      
        1 tttaggcctt agaataaagg ccacaggcgt tttctagcga ggtaaatcat aactctgaaa
       61 agaagagcgg tttaactttg tgggcggaag actgaaaaat atgagcttaa aagaggtcgc
      121 gcttatatcg ggagccgcag gaatactggg catcggaata ggatacttcc tacgatggtt
      181 ggttcttctt gcccaaaaag gctctatgga aattaccatc aagcagacaa tgcttgaagc
      241 caaaacaact gcgcaaaaaa tattagatga agcaaaaaag aactccgagc aacttataag
      301 agatgctaag gaggaagaaa agaacagatt atccgaagcc aagaagacag aagaacgtct
      361 tatcaaaaaa gacgagcttt tggataaaag gcaggtagat attgataaag aggtagaaaa
      421 cctaaagatc aaggtatctg aaataaggga gatcagaagc aaggttgagg aaatgcagca
      481 aaagactcag gaagagatgg aaaagattgc caaactttca gagacagagg caaaggagca
      541 gcttttcaat atcattgaga gaaaatatga ggatgatttc gtcgccagga tacaaaagct
      601 cgaaagagat ggcatagaca gattagacaa aaaagcaaaa gacatattgt ctatgtctat
      661 ccagagacta gccgcctcta ccgcatcaga aatgatgaca acgaacgttc ccataccgtc
      721 tgatgatata aagggtaaaa taatcggaaa agaaggaaga aatataagag ctttcgaaag
      781 agccgccggc gtagaagtaa tcatagatga cgcccccgga tcaataacca tatctagctt
      841 tgacccaata aggagacagg tcgccagaat ggcccttgaa aaccttattc tagacgggag
      901 aatacagccg gcaaaaatag aggaggttgt tgaaaaatct agacaagaag taaacaaaat
      961 cattaaagat agaggggatc aggccgtata cgaaacagga gtattaaatc tcgaccctag
     1021 gattgtcgcg atattaggaa gactgcactt cagaacaagc tacggacaaa acgtccttca
     1081 gcactctata gaaatggcac accttgcagg tatgcttgct caagaactcg gcgcggatgt
     1141 tgctatagct aaggcaggag ccctcctcca cgacatagga aaggccgtcg accacgaagt
     1201 cgcaggcaca cacgttgaaa taggaaagag gatacttgag aaattcgggg ctgacccaag
     1261 aatcataaaa gctatgcagt ctcatcacaa cgaatacgaa tacgagacta tggaggccgt
     1321 tatagttcaa acagcagacg ctatctccgg aggaagaccc ggcgcaagaa gagacagcgt
     1381 agagaattac cttaagagac tcggtgagct tgaggcgata gcaaactcct ttgagggcgt
     1441 tgagaagagc tatgctatcc aagccggtag agaaatccga gtattcgtgg ttccggaaaa
     1501 gatatccgac cttgaagcca aaaaaatggc tcaagatata gccagaagga tcgaacagga
     1561 tctaaaatac cctggagaaa taaaggtaaa tgtgataaga gagaccaggg ttacagaata
     1621 cgcaaagtaa gcttaaaact gaaagcgaaa atctaaaaag attattaaaa aacgacgtaa
     1681 acgtcgtttt ttaattttat attttaaatt ttacattgtt attttacatt ttgagatttg
     1741 catttttagc tataatctta aaattatcaa aaaagacctc aaactttact aaaaccccca
     1801 aatattacga aataatatac gacttttagg gccgttgcct aaaaaatggc ttgctgtata
     1861 attatattca aatggtcgaa acattttaaa ttattcttta actaattgag catacggatt
     1921 tgaatgcaga acgaggcgaa agctgaggct tgcaaggaga tgtgctaata gtacattgac
     1981 gagcaaatga aactttcaac aaagttatgc gtcaaatacg ttagtgaaat atatgaacaa
     2041 acaaggatta gtagatgtag tacatgccaa gcttggcgga acaaaagtag ccgctgaggg
     2101 agtagtagat tcaatcttcg aggctatcat ttctagcttg aagaaaggag acgatgtatc
     2161 aatcgctggt tttggtattt tcgctgtaaa ggcaagagct gccagaacag ctagaaaccc
     2221 aagaacagga gagactgtag aggttaaggc catgaaagtt cctaaattca gagctgcaaa
     2281 ggctttgaag gatgctgtaa aataacgctc tttgcattca aaaaacagcc ctcacaaaag
     2341 ggctgttttt gtttgcgcta gcggtaaaat aaaaccgcac atttctgtgt agcctgcccc
     2401 gtgaaattaa aaataattta acgcaaaacg aatactgcgt aaaaaaaatt cccgctttcg
     2461 cgggaataac tcttatctgc tatttcactg ggggtatttt tttatgagaa gttaattctc
     2521 atatctcaca acagcgaacc ttgtgtaggc tggccatatg ggatcatggg gaacgatact
     2581 taatcttttt cttccccagt tcatccaaag ctgtggcgcg cacaactctc cacgatagtc
     2641 gtggcaaatt gagccgagcg caacgacagg atatccggga aaaatttctt tcacatcgac
     2701 accaagagca aagaggggca aaagagtggt cggataataa tttttattat tcatccaacg
     2761 tctcacctgc cctgaagttg tcgggtagtc aaatgagatg agataaatat ttttactacc
     2821 aagttcgcga ccgttgtctc taataagagc ggcgagaact tcgggatcga cccatttcgt
     2881 cccggtcgct tttatataat catcaagatt cgggaatgcg acattcacag agtatggtcc
     2941 ggaaatttcc ggagactcgt agacaggatt atcgtgggcc tgtgcgggca gtggtcggtt
     3001 tatcatcaag agaaacatta ccgcggcaaa cgtgagcaaa agcagagcaa acaaaaattc
     3061 aataaaataa tttttcacgt gagtgcctcc tctctaattt aacccgccga aatttctcaa
     3121 cacctagcac caacataaaa aactgagaaa ttttgcattc ccccaacaag aaccaagata
     3181 gaaataatct ccatggaaat ttttgcgggc aagttttcaa attactctta acctaaaatc
     3241 acactagcat atttactagt gcgattccaa caaattcttc tgtgtgtcat tcccgcgcag
     3301 gcgggaatcc agaatattct actttacgtg atacttcttt cgaatcttcg acagctcctt
     3361 ctcctccact tcctccttct tttcctttcc ctcttctcct aattttttaa gttcttcatc
     3421 ggacaacttc accaactccc ccgctctctt ttccaaatac tcttcggtgt tcaagctcgg
     3481 gtcatccaag acttcttcga gcaaagcgtg tagagtgaaa cctatcttcg ggcccggagc
     3541 aagcttggtc acctccatta tcttctgccc gtttatctta agcatcccta cagtgaccgg
     3601 cgccctcatg gcctcctcta tcatagattc atatttacga agtctatatg gagtctctac
     3661 aggccttccc atcccgatcc tgtcgcaagc gcggacattc ataagctccc acacattctc
     3721 tttcccgacg tttctcacga tcctccttac cgccgacaac gttatcttgt ctatatccgt
     3781 gaaaaacata tgatttctga cgagcctcaa aaccttatcg gacgtttcac cgggatacct
     3841 tagcctttcc attatcttct taaccattct ctcccccaca acatcatggc catagaatgt
     3901 gaaatctttt ttctcttcgc tccagcccct tgttctaggt ttcccaatat cgtgaaacag
     3961 cgcggcgagc ctcacctcca gagaccattt cttttctccg gcatgaccga gcgcgcgaag
     4021 attatgctcc cacacttcat aaatatgatc gctgttttgt ttcacaccta taccctcttc
     4081 aagttcaggg acaatgtaac tgagcaaccc aaatttttga cacatgacaa cgccgacgac
     4141 agggtcgtct gacattatta ttttagaaaa ttcatctcgt attcgttctt gagaaatttt
     4201 ttcaagaaga gctgcatgtt ttattatcgc ctcacttgtt tcactggcaa ttgtgaaacc
     4261 aagttgaaca gaaaatctaa cagccctaag cattcgaaga gcatcctctc caaatctatc
     4321 ggacgccaaa ccgactgtcc ttaagacttt gtcctttaga tcttcttgtc ctttataaat
     4381 gtcctttaga actcctgaag aaggattata ggcaattgcg ttcaccgtga agtctcttct
     4441 gtgcagatcg tcttcaatat ttttactaaa cttaacttcg tccggatgtc taaaatcaga
     4501 atactctgat tcaactctgt atggagtaac ctcaattact cttaacgact ctctaagacg
     4561 caaatctgac tcccctccct tgtccgcaaa aacttctttt ttagaagttt cggcggatcc
     4621 aatctctttc gcgatctcct catttaccac accaactgtt ccgtatttat tttcataaaa
     4681 agttttcgga aataattttt gtatttcctc cggtgtcgca ttcgtcgtca catcccaatc
     4741 tttaggctct ctcttcatca agacatctct tacacaccct ccgacaagat acgcttcgaa
     4801 tccacccttc tccaaggttt cggttgtata taaaacttct gtcggtatgg tgagtttcat
     4861 cttaattaaa ttacttatta tacaaactat gatctggaga aaatatttgt attaaagcgt
     4921 ttcgtaaaga ttcttccaaa ctgatctctc tggcaatcat gccgccggaa tagttgcccc
     4981 aggtctcact cggcatttta ccatcttcct cctctatcat tgccataaca tcacaatgtt
     5041 ccaattttaa ttcgtccatt ttctttaata tgagatccgg aacttttaga ccgcttgaat
     5101 aaccataaaa actcttctca tctttatcta atattgcaca ccaagcctct tcataaatat
     5161 gaccgtgtct ctcaaccagc ccgctctcta gtccgataaa aatatctccg tctttaatgt
     5221 gcgccttcgc gtctctcgcc cggttattcg ccccatcaaa agtttgtttg tcgtaagggg
     5281 tatcggggac acctgaataa gacggatacc cggtgataaa aagattccct cctccaacga
     5341 gctgtctgat tattttttcg gcaacttcga tctttctctt acttgccgac ccgatcacaa
     5401 atttcatatt tggtttttcg tattatagcg atatgcgcat aagatccggc cacaaaacac
     5461 tcaggccttt cctgacttgc gcgagagtac aagtgatcat aaaacttctt ttgtgcggat
     5521 agcgagaatc ggactcgcgt ctcaaccttg gcaaggtcgt gttctaccac taaaccatat
     5581 ccgcgtcaag tctttcaaaa tctctacttt ctttaatcga ttatagtatc tcctccccca
     5641 aaaatcaacc ccgaaagagc ggctttgata tttgatcaaa attaatgtaa tctttactca
     5701 ttgaagatgc tcccgtggtg aaatggatat cacatcggtc ttcgaaaccg ctgttggggg
     5761 ttcgagtccc tccgggagca cagcgaccga gcagaccaac tgcttggtct gcgaggggga
     5821 cgagaaaagg ttctccgtta tgttttagaa atatttattt ctaaaatatc ggaaacctat
     5881 accgtcgccg taggcgaaga gtcccccctc cgggagcaca aaatttaacg tcagttaaat
     5941 ttttgagctc ccgagcaaag cgcctgcgcg ctttgcgtga ggggctcgaa aggcgcagcc
     6001 ttatttttgt tttgcgaagc aaaggcaaaa atggcgagcc ggggttgcga aaaatttccg
     6061 tcaggaaatt tatttgcgac cgagttcctt gtttaacata ccttattgcc gaagtggtga
     6121 aactggtaga cacgcacgct tcagaggcgt gtgagagtga tctcgtggga gttcaaatct
     6181 ccccttcggc acaaattaat agccatcggc cattatctaa tacgaggaga tttgaactct
     6241 aagagagtcg gggaaaaatg gttttccccg tagagtaaaa cattaaaaaa accgtgggtt
     6301 tttaaagcgc aggcgctagc cgagcagtaa aattaccccc tccagcacaa caaaagcccc
     6361 atatcggggc ttttagtttc acaagaaaca tttaaattat taggtagcaa caaaaaattc
     6421 taggaatatc tttttcccta aacattgccg attggccagc agagtagata atattttatt
     6481 agtctgtgat agtcgcaagg ctaacgaata actctattgt aaagagttaa atttaataac
     6541 cgaggatctg ctagaacccc gcgtcacaaa ccccctcttt tttaattgtc atatcaccag
     6601 ttttgcgaaa cccacctacc aatgtcctat agaattttct atcggacatt aatattttat
     6661 catatttttc tataagacaa gcagttattc tatgaaaaag tgtggataaa cctgtgaatt
     6721 gtgtgtataa aggacattct tatctagtcc tttatctgtc ctatagacct ttttccggtt
     6781 atgtctttat ttagggccat attttagtct attgcgagac aaatagcgaa ctctacatcg
     6841 tgtatcccct atggattaac ccatttttat gtttcacgtg aaacttttta atgtgtttca
     6901 tatgaaatct tttgtgttta tggcttatat tttcacatga aacacagcaa tagatgtatt
     6961 taaagccttg ttatagaagc ggattatgaa cctattgccg ttttttacag ttaagcataa
     7021 tattccaatc atggcttggc agttggatgc agaacgagga gaaggcgagt attgtgacga
     7081 agatgtataa cacatacatt gaggagtaaa cggaaccttc gacaaagtta tgcgccgact
     7141 gacgagccat gacatggcta ttcaacaaga aataggacaa ctaggtgaag atatcgctgt
     7201 gaaatacctg aaaaacaagg gttattcaat aattgagcgt aattattgga aaaaatgggg
     7261 agaaatagac attgtggcag aaaaagcttg ccttatacac tttgttgagg ttaaaagtgt
     7321 ttcatatgta acgccaaaca cgggcgttac atatgaaaca cccgcttttg acccgcaaga
     7381 tcatatgaat aaagaaaaga tgcatagact gaaccgagta atagaaacct atcttgcaga
     7441 taaagacatg gaaaatgagt ggcaggttga ccttatgctg gtttatttaa acaaatcaac
     7501 aaagacggca aaggtcaatt tgatagaaaa tataacgctt taataacaaa aattgcgttt
     7561 tttaaaatat ctgatactat gtttgagtaa cagaaacgcc gttaggcata atgtttcata
     7621 aagggcagta gtttgatttt ataataacgg agtgtggtgt aacggtagca taaatgcttt
     7681 gggagcattc agtccgggtt caaatcccgg cactccgatt gattgcgtag cgagcaatca
     7741 gtgtaaaaga gccaactgct tggctctttt agggatttga aaggcttctc ccttatttct
     7801 gaagagcttt gctctgaaga aatgggaaag ccatactgtc ctcgtagagg aaaaacccgg
     7861 cactccgaat tttacagagt aaaattctag tgcagcaaag cgcctgcgcg ctttgcgtcg
     7921 ggatttgaac ggcagagcga tgttttgtga gcgtctggcg aacaaaaccg cgagccggtg
     7981 cccaacccga ggaggcggaa gccgacgaga ggtgcgggct aatccctgca acttgtgacc
     8041 aaaaccgata cttccatagg gaaaaagtcc caacatgttt ctcatgaaac acaaaaatac
     8101 ccttaaatta tggcaatttt aaacaaaaaa gactattttg cccaattatt caaacttccg
     8161 ctggcggcag gtgggctgtt ttttaacaac aaaaacgaaa tcttactttt aaaaccaact
     8221 taccgtgacg gttatctgat acccggcggg atggtcgaag aacaggaatc tcctctaacg
     8281 gctctcaaaa gagaaataaa cgaggaaata ggccttcaaa tagaaaaagg ggatatattg
     8341 tgcatagatt acaatcccaa aaacgactca tacaattttg atgattcatc aatccaaata
     8401 gttttcgact gtggcacctt aaacaaagag caaatcaaca aaataacatt gagcgaggaa
     8461 cactccgagt tcaaattccg ttctatcgag gaggcggtaa aaatgtgcaa tccaaagttg
     8521 gctaaacgcc tacctaagtg tattgaagct cgaaaattga atagatgtgt ctatcttgaa
     8581 gagggaaaag ctgtctcgta aaaagcatcg ggcccggcac gtttcccatg aaacaccctc
     8641 aaataatcct atttatctag ggccccttac atgtgaaaca agattattgt tattacatat
     8701 tttctttctt aatttctgga ttgccacgct tcgctcgcaa tgacaaataa agcatcgcgt
     8761 agttcaatgt ctcctaaaat tgtcatttcg actttatgga gaaatcgctt atttaatcaa
     8821 ggggttcctc gacaagctcg gaatgacaag ggtgaagagg ccccagttag aagttcaact
     8881 aaaacccaaa attaacttgt cccgccagga aagtccgacc agcgttttgt ggtttttgtc
     8941 tcttttgcaa ccgaaaccgc ctgcggctat aaataatggc gcacccccca gcaggaaggt
     9001 tcgaatgagc tacaattata attagtgttt cgtcgcacgg tatccgccta tcggctccct
     9061 tatttctgat ataactccca ttgataaacc tcctgcgggg taaacttgca tttaaataac
     9121 ctttgtgcta tcgtgtcggt atgctaaaga aagacaacaa ggccaaaata atcaaaaagg
     9181 ccagaaaaca cgaaacagat actggatctt cagaggttca gattgctttg ttaaccgaga
     9241 atatcaacaa actaaccaat cacttgaagg ctaacaagaa agacttccac tcaaggagag
     9301 gacttcttca gatggttgca gacagaagat ctcacatgaa atatctcgag aagaaagaca
     9361 aaaaaggatt tgacgctctc gcagaaaaac taaacttgcg aaaaacaagc aagatagact
     9421 aaaacaaaac gtagtttaaa atggctatca tcaaacaaaa aagtcagagg gttggggtct
     9481 tcatagatac tcaaaacttg tatcacagtg cgcgtaatct atatggagcc aaggtaaact
     9541 ttggcgctat tttggctgac gctgtggccg gtagaaatct tgttagggcg attgcatacc
     9601 tcatcaccac ggaaagcgga gaggagaggg gattctttga ggctcttgag aaacttggaa
     9661 ttgaaacaaa aacaaaagac cttcagatat tcttcggggg agccatgaaa gccgattggg
     9721 acgttggtat tgccgttgat gctattaagc tcgcgccaaa attggatgcg atagttcttg
     9781 tttccggaga cggagattat attcctctag tagaatacct taaagccaca acacaagttg
     9841 agctggttgc attcggcaaa agctgttccg caaaactgaa agacgctgtc gatgatttca
     9901 tagacctcgg aggagatcca aaaagatatt tacttggaca gaaaccgata aaaaagttta
     9961 gcaagggtgg gcagagtgcg gcgggggcag ggcaggagaa aaaagtttag tattattcgc
    10021 agttaattaa tcggggtttg gtccatagat agatagcatt gaaataaaaa tttcaatact
    10081 agaatctaag ggtcaaattc tcaaaaaata atatgcaaag aaaagaatat tctttggttg
    10141 ttggtggtaa aacagttacc gcgatttttt ccgatatggt cgcgcaggca aacggttctg
    10201 ttgtaatgaa aataggggaa actagcatat tggccacggc cgttatgtct agcaacttaa
    10261 aagatggttt ggactatctt cccctcacag tagattacga agaaagatac tacgcaagcg
    10321 gaagacttcc gggagggcag tatgtcagaa gagaaggaaa gccatccgag gaggccattt
    10381 taaccggaag aatcgtcgat agaacaatta gacctctttt tgatcagagc atcagaaacg
    10441 aaattcaggt tgttgtaaca acactttctt tggacggaga aaatgatccc gacacaattt
    10501 ctgtcctcgg agcatctctc gctattgcga cttcaaatat cccttggggt ggacctgtag
    10561 gctctgtaag ggttatcgta aagaaagatg gaacgctcgg aataaactcc ggtcattcag
    10621 aaagaaaaga tattcttctc gacagctata tctgcggacg agccggcacg attaatatga
    10681 tagagacaga gtcgaaagaa gtcaccgaag acaaaatcaa agaagtcttc gagctcgcga
    10741 tgtctgaaat cacaaagata gaagattggc agaaaaaaat tatcgccgag attggtaaag
    10801 acaaaaaagt ttttgagaaa aaagaacttc caaaagaggc aatcgaactt tttgaaaaag
    10861 aaatcggttc caaaatggag tctatcgtat tcagcggttc cggaagcaag atgtctcacg
    10921 accttctcca tgaatggttg gatatttata cagaaaaatt tgaggactcg gcagacaaaa
    10981 acttggccgc aggatatttt gacgacaaaa taaacgacct tctacacgag gaggccataa
    11041 aaaataacag aagggcggac ggaagaaaga tggacgaact cagaaagatc gaggcgtctg
    11101 tcggaggaat ctcagacata attcacggat gcgggctctt ctacagaggg gagacgcaca
    11161 tgctttctgt cataacactt ggcggaccaa aagacgccct ccagctagac ggagccgaag
    11221 tggctggtga gaagttcttt atgcaccact acaacttccc tccatattca tccggtgaaa
    11281 caggaaaaat cggtaacaca aatagaagaa tgatcggtca cggcgcatta gccgaaaaag
    11341 ccctcagagc gacacttcca tccagagaaa cattccctta cacaattaga attgtttccg
    11401 acacaatgtc ctcaaacgga tctacctcac aggcagccat ctgcgcctca tctctcgccc
    11461 ttatggacgc cggagtgcct atttcaaggc ctacagccgg tatcgccatg ggtcttatga
    11521 tggattcgga ggggaaatat aaggttttaa cggatattca gggacctgaa gaccattatg
    11581 gagatatgga tttcaaggtt gccggaacaa gagtcggagt agccgctata cagcttgata
    11641 taaagatgga tggagtgcct gtaaatgtcc ttgtagaggc tatgatgcag gccaaaaaag
    11701 cgagacttga gatccttgat gttatagaag gcgcgcttcc tgctcctaga gcagacttgg
    11761 caaaatcagc ccctagaata ttcaagattc acattaatcc agagaaaatc ggcgctgtta
    11821 tcggccccgg cggaaagatg atacaccaca ttacagatac gacaggcgca gagataaatg
    11881 tagagcaaga cggaacggta ttcatcacag gaaaggctga aggagccgcg gcggcaaaga
    11941 aggccatcga agaattgacc agagaattca aaatagggga cacaatgatc ggtgaagttg
    12001 taaaactagc cgacttcgga gctttcgtaa aaatcggcat ggatacagac ggacttgtcc
    12061 acgtttccga gattgctcct ttcagagttg ataaggttgc cgacttcttg aaagtgggga
    12121 tgaaggttcc tgtaatcgta aaagataaag aaggggacag aatcagcctc tctataaaga
    12181 gaataaagcc tgatctcttc aaggctcccg catcggcatc aatgcctcca aggccaccga
    12241 tgacacctcc ggcagctacg ccaaagatat aaatttatag aaaaaccacc cgatgggggt
    12301 ggtttttgtt tttgtgatta tattatttta gccctttctt caacggtttc aaacgtttct
    12361 cctttaccgt cacttttctt cagatgggga tagagaagta tcaacgagag ggctccaaga
    12421 agcgcccaaa gccccgcccc aacgaataaa acgttaaacc catatctatt agctattatt
    12481 ccaccgagtg cggatgatat cgcagtggcc acacccacag aaaagacgct ctccaaactc
    12541 cactcaaaac tagtcctgcc gctatctata tgcctggtga atattccata ccacgcagga
    12601 accgcaaacg ccatagaagc acccaaaaaa gcctctatcg cgtatatgtg ccaaggtagc
    12661 gtcgcgaata tatacataaa tagagaaaaa ctagcgatta cttggccgca gacaagaaca
    12721 acgaagtcat ctttctcccc atccgtcttg tccaaaaact cggcgatagg caattgaaga
    12781 aatgattttg caatccaata aactgaagcg gcaaatccgg ccacctcggc atttccccct
    12841 tgaatattat caacaagaaa aatggcaaat attggagcag ataatccaaa agctgaattg
    12901 ataacaaaat ccgacattac taaagttttg atgacactat ttattttcat cccgtagtag
    12961 aattttaatt tttaaaaata tccccaacct cataaaaaat cccataattg cctatttggc
    13021 gcctttccct ctcttaaaat ttcactacag gattttcatg gattaattat agcaaaatca
    13081 ttaaccctgt taagtaaaat ttttaaaaaa aatttaatca cgattaaatt tttgctaaac
    13141 agggtaaata aaaaccgcct ctttcgaagc ggtaaaaaca actatagatt tgcgtaaacg
    13201 gtgcgcataa cccaaattcc gtttctgctt tcacagattc ttttgtaggt gggttcatct
    13261 accaaaacaa tgctcggaac accgtgcacg accgatcctt cctcacgcac gcgatattgc
    13321 ttgtaaccgg caataaacgc ctcacgatcg gtatcgttta aatgcaaggt gcacccatcg
    13381 ggacaattgt tacaaatcca aagctgccga accaccctgt atttattcac tgagcatctc
    13441 caatctgttt aatttcgtaa tcaatccaga gtccgataat gtattcctta tcataaataa
    13501 aatgagtgga aataatcttc ttgtccggat tattcttgac ccaccatgcc tttttgataa
    13561 caaggtccat gacatgaatt ctaggcgaag cgaaatggtc agaaacatgg atgacagcag
    13621 tgcactcctt gtcgtcgtcg atgaaagaag agtaatctcc ttcagcgtga ctggcggtaa
    13681 tgacaaacat gttcatcgaa accaagatag tgaagcacag ggcaaaaaat aggaatcttt
    13741 tcatcggaaa acctcccttt ttatacagtg accacatcat aatctgcgct aaaagaccaa
    13801 atgccatctg tgccggaaga aatgtaattg agaacttcct cgctcacaaa aacaatctca
    13861 ggcgcaccaa ttggaatacc gaaactactt tctacatgtc tccgcgcaaa tgcttctcta
    13921 tccctttcgt tcttatggag agaagaccag tccgaagaat atgaaaagga ccacctttga
    13981 cgaacgaccg cgtagagcat gaacgcctcc tctatttttt atttttcctg aacgttttaa
    14041 ggttatatcc ctggaaccga agccatcttt tccactctcg aagctctttc ttcataagag
    14101 gcttggtcat atctaagccg ggattaggaa tcatttgatt tcttgccgcc cacatgagcg
    14161 cttgaccagc ctcatgttcg aactctatcc ccgacgtcga taagaccgtg gcttcataaa
    14221 ctatttgggc taaggttgct aatcggtctc gattaccggc gacgccagag agagtcttat
    14281 aaatttggtt attgatatct ttatataatc ccgacaattt atttattctc aaaagatcaa
    14341 aagccaaccg gtctaaatat ctctgaccga aaccccttgc acgaaccccg ctgtccgcaa
    14401 agatagtgat attggaacga gaattgtcgt ccttattctt tccagccttc ttctttggca
    14461 tatctcggac ctccttttaa atgttctttt tctaaagaga gattagcata aaaggggaaa
    14521 agggggaatg gcacttgaag caaaacaaaa acagagctta cgctctgttt ttggctgcaa
    14581 gtgcgggatg ggagaatcga actcccaacc tcggtttgga aaaccgatat tataccattt
    14641 aaccaatccc gcttagataa actacaaaaa cactctaatc ccaccaatca attatgnnnn
    14701 nnnnnnaact tatttctcca aataaactct acccatttaa ccccaaagga aagtccgaat
    14761 atcttttatt ttttaaaaag acattcaatt ctcggaatcc gccttcggct tctttttgtg
    14821 gtcacctatc ctttatagat agacttccta cggggcaggc caatcccgct tatttaatta
    14881 caaactatca attaacaatt acgaattgaa cggcccaact aactacctat ttttaataca
    14941 aaaagggcaa ttttgcaagt ttaccccgtg aaattcaatc tcgattacta cgaaagtcta
    15001 ataaatacat ggattcccgt cttcacggga atgacagctt tgactcttat ttcgccgaga
    15061 gatcgcaaca ccttatcggg tatttaacga cctgtcgcgc tttttgcgca ggacgttacc
    15121 cctaattgca aaaaataaaa taaattattg gaaaaaatta ttcttgaaat ccctcgagct
    15181 cttggcatgc cagacattta tcggcgcctt ctgctcccgc gcaggttgtg caatccattt
    15241 tttcgttaca aagagggcaa gctcccggat actctgcttc atttttacaa gttccgcaaa
    15301 cgtacatttg tttaaaaaat attacgcgat gctaatagac gaactttata ttctcagtat
    15361 acacagagaa aaagttgggt caatcctgta gtgaaatttt ggccttgata ttttttataa
    15421 aaataaacgt taaaaataac ggatattttt gagccaaaat tatactacgg gatcaataaa
    15481 atagttttcc acaaccccgt agtagagcaa agctcgttac agggcagacc acgactatat
    15541 aaagaaatct cacaaacgaa gattcttacc agtttgactt tgaatatctt aaaagtatta
    15601 ttagttcaaa gcatagaaca ttgaaattca tttgccaaaa ttaaaacgat attcccaaaa
    15661 gctaaagaga caaatcagag aggaggggcg acaagtgaaa ataaaagaag gtcagttgtc
    15721 taaatggaac aattacgttg cagaacatca aaccggctat gacagaacaa ttattatgtt
    15781 cacagaaacg attgccaatc tgatagagct aaaccgcgat cgcttctgga gctttaatat
    15841 ttcacttatt agcgctatca gggaagccgt tgtcctttgc ggaataaaaa ctgacgaccc
    15901 ggaactggcc gatgcaatgg tcaatgaggc gtttggatta ctttttaatt attgggagag
    15961 tggttggata atggaggaat actccttcag agacaaaact ctctatcatg aattccgtat
    16021 tacatggacc cttcgttagc aaaacccccg cctaagcggg ggttcttttt tatataaaca
    16081 cctcctttga gcaaagaagt gtttatatta tatttttagg aattctttga ccttagccac
    16141 aatctcgtta gaggtgaaat ttgatttgac cataaagtct ttcgcgccca gatccttcgc
    16201 cttctttata tcctccattt tgtccaagtt tgagagaact aaaacaggag cattagcgaa
    16261 attcgggtct ttttgtatgg cggcaagaac atcaaaaccg tcaacatcgg gaagcaaaag
    16321 atccgacaag acaaggtcag gcctgattgt ctttatcatt tccagggcgc tcttgccgtc
    16381 aacggccacg tcaactgtaa ttttttgctc ggacaatttc tggctcagca tttctctgag
    16441 gaacctgtcg tcttcaatta aaaatacttt cttgtccatc ctgttaaaaa attaatatta
    16501 taaattattt attttctctt ggcgccgact gcagaagagg caaaaatatc tcaaccacag
    16561 tgccttctcc ctctttagat tctaacgtta tactaccgtt atgtctagta ataatgcttt
    16621 ttgaaatata catacccaag ccggaaccgt ttttgtatag atccttggca tttttggccc
    16681 taaaaaattt agtaaaaacg cggcttttct cattttccgg gattcctatt cctgtatccc
    16741 ttattctaat gatagccttc cccgcttcga gccttgtaga aatatctacc gaacctccgc
    16801 ttttagtata ttttatagca ttatgaataa tatcgt
//
LOCUS       DAOTEX010000008        15809 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3118
            NODE_2527_length_15809_cov_4.197601, whole genome shotgun sequence.
ACCESSION   DAOTEX010000008 DAOTEX010000000
VERSION     DAOTEX010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868142
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 15809)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15809)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 883
            CDSs (total)                      :: 833
            Genes (coding)                    :: 829
            CDSs (with protein)               :: 829
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15809
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2527_length_15809_cov_4.197601"
                     /isolate="AMR_MDS_3118"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(91..225)
                     /gene="rpmH"
                     /locus_tag="P5056_00935"
     CDS             complement(91..225)
                     /gene="rpmH"
                     /locus_tag="P5056_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004081758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HRY62319.1"
                     /translation="MSKTYNPKKRKRAKTHGFLVRKATPGGKKVVQRRRRKGRAKLTV
                     "
     gene            549..1937
                     /gene="dnaA"
                     /locus_tag="P5056_00940"
     CDS             549..1937
                     /gene="dnaA"
                     /locus_tag="P5056_00940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HRY62320.1"
                     /translation="MTDHKKIWEDALLEIEMGVSRANFSTWFKNTYISKQEDGTIYIN
                     VPSAFVKDWLQNKYHKLILKALRDTLPDTRGVEYIIQKDIQPPKEHAGELEQKIPSAQ
                     LRLNEPYVSRDDNLNPRYIFDGFIVGPFNELAYAAAQAVIKNPGATYNPLYIYGGTGL
                     GKTHLIQAIGNHIKKSKPNAKVYYLASERYVTEIVNSITNNTINITKEKYKKYDALII
                     DDVQFFSKKDKVQEEMFHLFNDFYNNNKQIIFSSDKPPKYIQDLEERLRSRFEGGMIV
                     DISKPEYESKMAILNHKLKTLNYSLPAEILDFIASSVQESIRELEGILNAVICQSQLK
                     NKELTVNDVKTLLKNNSKQQKSLSIKDIVRIIAEFYNIEEKHLYEKTRKREVVKPRQI
                     AMYILRENFNSSYPQIGEKLGGRDHTTVIHACEKIKEDIKKSDNLMREIEQIKSLLFG
                     PENKISSSAGVV"
     gene            2240..2623
                     /locus_tag="P5056_00945"
     CDS             2240..2623
                     /locus_tag="P5056_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004072159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HRY62321.1"
                     /translation="MGDTKKIKSFTDLIAWKEGHNLVLKIYELTKLFPKEELFGLVGQ
                     MRRCAVSITSNIAEGFSRHSYNDKAHFYSISLGSITELQNQLIIARDIKYIEKSICND
                     VYEQSIKVHKIINGLIKNSRIIHNS"
     gene            2649..3749
                     /gene="dnaN"
                     /locus_tag="P5056_00950"
     CDS             2649..3749
                     /gene="dnaN"
                     /locus_tag="P5056_00950"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HRY62322.1"
                     /translation="MKAECIKDKLKEAVFQVGKISAKNLSLPILESILIEAKGSEIIL
                     KATNLDIGLEVKVPAKVEKAGSVAVPGYILAGFIQMLESDNVVKLEAINNNLSVSTKK
                     NSTLIKSFPADEFPLIPRVEKEESLTVEASKIVSGIRAVSFAALGSDIKPEISSVYIY
                     VDNGSLYFVSTDSSRLAEKKFDVKADSDFSSSIIPIKNATELVRIMENVTGNVLLEFN
                     KNQMSVSAPNFYFTSRVVGGIFPDYRQIIPKDKKTEVVVLKQELVDSIKLSSIFSDKF
                     NQVTFKIIPSDSFFEISSRNQDKGESVTKIEATLEGEDIDMSFNSRFILDCLNSIKED
                     SVSIAFNGHQRPVIIKGIGDESFTYLLMPMIK"
     gene            3876..4136
                     /locus_tag="P5056_00955"
     CDS             3876..4136
                     /locus_tag="P5056_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62323.1"
                     /translation="MQQLSSLLERFKRFSRPNIEIREAVSKAIKSEIGAIVSEKDVSI
                     KSGVAYVKTHNSILKNELFLKKKNILDAVQNVCGHKSISDIK"
     gene            complement(4215..6749)
                     /locus_tag="P5056_00960"
     CDS             complement(4215..6749)
                     /locus_tag="P5056_00960"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HRY62324.1"
                     /translation="MKKTTKVIRFIKKEIERILIVSAILLFLATGGGILWIANLKIPD
                     FGSFESRKISQSTKIFDKTGKILLYDIHQDIKRKVVPYEDISKDIKNATVAIEDSDFY
                     NHYGIKPMAILRAVFVNLTSGSKVQGGSTITQQVIKNSILTSEKSYTRKIKEWILALK
                     LERVMDKEHILSLYLNESPYGGTTYGVEEASQRFFNKSAKDVTLAEAAYLAALPQAPT
                     FYSPYGQNKDKLDERKNLVLNRMVELNFISKEEAEKAKNEQVVFISKEEKGGIKAPHF
                     VEYVRSYLENKYGKEVLETGGLNVTTTLDWELQKEAEQVVSDYGKINEEQFGAKNAGM
                     IGIDPKTGQILVMVGSRDYFNTENDGNFNVTLAHRQPGSAFKPFVYATAFKQGYIPDT
                     VLFDLPTEFQTTCTPEGLPISSEVDPADCYMPENYDGIYRGPIKLRDALAQSINIPAI
                     KLLYLVGIENAIQTARDLGITGLTDANQYGLTLVLGGGEVTPLDITSAYSVFANDGIR
                     NPATPILKVVDSSGSILEEYSDNGVRVLDENVARQINNVLSDFNAKLPAYGENSPLHF
                     YNREVASKTGTTNDTKDAWVVGYTPNFALGVWVGNNDNTPMVKKVAGMITAPMWRAFF
                     DKALLTLPAEKFTKPAEMNTDSLKPVLRGDWRGGFPYTIDKASGKLSTEYTPPEMKEI
                     KTVTQVHTILYWLNKKDPLGPAPIYPQQDPQFNSWETSVRRWAAEQKIQEESISVIPT
                     AADDIHGPNFAPKINIVSPDQNREYPLNEKIGLFFTSSPSKYPITQADFFINDTFIGS
                     SKNAPFSFYFVPSDIPQIKNVNNLKVIIYDSVRNSGENNSIFKVAI"
     gene            7104..8294
                     /gene="tyrS"
                     /locus_tag="P5056_00965"
     CDS             7104..8294
                     /gene="tyrS"
                     /locus_tag="P5056_00965"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HRY62325.1"
                     /translation="MENKEQINDVITRGVENIYPSADFLRAKLERGEKLTIYLGIDPT
                     GPTLHLGHAIALKKLSEFQALGCKIIMLIGDFTAMIGDPTDKKATRKQLTREEVLNNC
                     KLYKKQASVFLNFKGDNPAELKYNSKWLAKMNFEDVLNLASKMTVSQMLERDMFENRM
                     KEGRPIFLHEFMYPLMQGYDSVAMDVDGEVGGNDQTFNMLVGRNLLKELKNKEKFVLT
                     MKLLTDNSGTKMGKTEGNMLSLIDDANMMYGKVMAWEDSLMWNAFVLCTNVDAEEIEK
                     MKAEVSGGKNPRDIKMRLAYEIARIYYGDSKAKKAEKEFVKTFQKKETPDEMEEIKVN
                     VGDLLADVLVVRGFVKSKSDFRRLVEEGAVTIDGEKISDFQIKIEKTNVVLKIGKKRF
                     IKLI"
     gene            8302..8913
                     /locus_tag="P5056_00970"
     CDS             8302..8913
                     /locus_tag="P5056_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62326.1"
                     /translation="MAFSPEGRSGETGGTLEWGVFGGIGKGKVMTAIKEMGLTTEEFL
                     KLKDKGYLPYDKSIDLVKRIQNVTEKHNPANPEKMIVRDIKDFLEEFLKKKSENVRFY
                     SALKTPLDIMHGVDAFFEIDLEKEEGKEQQKPVVVTMDITMRAKEGDKDQKANVLIRM
                     PDGAPLSADDDYEAKVKQIARAVKDAFENIKSGDKFFKTAITI"
     gene            complement(8990..9172)
                     /locus_tag="P5056_00975"
     CDS             complement(8990..9172)
                     /locus_tag="P5056_00975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62327.1"
                     /translation="MPKPKKAQNEEGGAEVYESFDENLDAALSEDALEGITASDDDFD
                     PETALGLDIGSGSDGI"
     gene            complement(9260..9751)
                     /locus_tag="P5056_00980"
     CDS             complement(9260..9751)
                     /locus_tag="P5056_00980"
                     /inference="COORDINATES: protein motif:HMM:NF014166.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HRY62328.1"
                     /translation="MRVIGVDPGYDKFGVAILEKRNGQDILIFSDCLRSSAKLPFSER
                     LFTIGSDFQHLIEKFKPDALAIEKLFFTTNQKTAMGVSEIKGIATYIAKVNGLKTYEY
                     TPLQVKMAMVGYGKANKDQVSKMVPLLIKNFSEFKKNKIEDDEFDACAIALTCLATER
                     VAK"
     gene            complement(9853..10389)
                     /locus_tag="P5056_00985"
     CDS             complement(9853..10389)
                     /locus_tag="P5056_00985"
                     /inference="COORDINATES: protein motif:HMM:NF013841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRY62329.1"
                     /translation="MSGHNKWSKIKNKKGAEDAKKSKVFAMLAKKITVESRLAKGDKN
                     SPSLRKAIEKAREANMPNDKIDGAVAKGTGAGGAEMTEVRYEAYGPGGTAIIIEGITD
                     NKNRMTPEIKHIISDNGGTMGAEGSVIWAFEKADGEWVAKMPMELSDSDKTALENLLE
                     KIDNHEDVENIYTNISNL"
     gene            complement(10530..11528)
                     /gene="ruvB"
                     /locus_tag="P5056_00990"
     CDS             complement(10530..11528)
                     /gene="ruvB"
                     /locus_tag="P5056_00990"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018746299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HRY62330.1"
                     /translation="MELEDTSSQKNEVFLDKTLRPDRWDDYVGQEKIKENLKILLSAA
                     KERKQPPEHLLFYGPPGLGKTTLAHLISKENNSQIKITSGPAIEKVGDLASLLTNLSA
                     GDILFIDEIHRLNKLIEEVLYPAMESGMLNIIIGKGPSARTIQLELPPFTLIAATTKI
                     ALLSSPLRSRFSGGTFRLEFYTDEEIERIIERSSKILGVSINKDAAKEISRRSRFTPR
                     IANHLLKRCRDYAQVKQQELDKKSVGEALDLLGIDHAGLNIIDRKIIETIINKFGGGP
                     VGLGTIGAAISEEVGTIEEVNEPYLIQLGFLERTPRGRGATPKAYKHLNIPPPQDN"
     gene            complement(11996..13693)
                     /locus_tag="P5056_00995"
     CDS             complement(11996..13693)
                     /locus_tag="P5056_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HRY62331.1"
                     /translation="MPTFDDTQSLKKLEETKIREAEDLAQILSGKYGIPYLDLSTVSI
                     NTDALRLIEESVAKEAKIAGFQMTGKKVSILVFSPNNPALDEALNDLKERGYSPVLYM
                     GSERSLERAWGRYKEISQAVETKAGLVDISPELLAEVLAKTKAVADVKNQIKELTAGN
                     KSQGVSRILEIVIAGALATKASDIHLEPQEEFVRLRYRLDGMLHDVDFFDHNVYKLIV
                     SRIKLISALKLNVKKSSQDGRFTIVAEDRDIEVRTSVLPGVYGETIVMRLLDPNSISV
                     NLEDMGIEEKLLKVFLDEIKKPNGLILTTGPTGSGKTTTLYAFLRKVNDSETKIITIE
                     DPVEYHMKGLSQTQVSEDSGYTFLEGLRSALRQDPDIIMVGEIRDPETAKIAINSALT
                     GHLVFSTLHTNDAAGAIPRLVDLGVNPKIISSALTLTIAQRLVRKLCPECKKATTPKE
                     EDAKMIENVSKLIKEKRPDIAVPDGKQVWVAGEGCEFCNGVGYKGRTGVFEAIIVDAD
                     VSRVITGEVNARDIKIASHKQGILDMREHGILKVLQGMTSIEELERVVELRLAEELY"
     gene            13913..14368
                     /gene="tsaE"
                     /locus_tag="P5056_01000"
     CDS             13913..14368
                     /gene="tsaE"
                     /locus_tag="P5056_01000"
                     /inference="COORDINATES: protein motif:HMM:NF014426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HRY62332.1"
                     /translation="MKKITKNLQETHDAARDFAASLSMAVDKEGESDGAIVVALSGDL
                     GSGKTSFVQGVAKYFGIKDKIVSPTFVIQKRYELKGHKNLKNLVHIDAYRLLKPEEIT
                     RIGWNNLIKEKGNIVFVEWPEKIEPLLPKGHKKLVFEFIDPEKRGIEGV"
     gene            14543..14821
                     /locus_tag="P5056_01005"
     CDS             14543..14821
                     /locus_tag="P5056_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019118595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62333.1"
                     /translation="MNTKTLINIKTDKTIKEKASKVAAELGMPLGTIINAFLRQFIMD
                     KEIHISASYRPSPMLKTIIAEAEMDIAEGKGLSPVFSKAEDALKYLRS"
     gene            14831..15088
                     /locus_tag="P5056_01010"
     CDS             14831..15088
                     /locus_tag="P5056_01010"
                     /inference="COORDINATES: protein motif:HMM:TIGR02385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin, RelE/StbE family"
                     /protein_id="HRY62334.1"
                     /translation="MRIQFHKRFIKQHKNLKKIWDKFDERFILFTKDPNHPLLNNHPL
                     HGKYDNYRSINITGDIRAVYKEVNKDLVIFIALGTHSELYS"
     gene            15149..>15809
                     /locus_tag="P5056_01015"
     CDS             15149..>15809
                     /locus_tag="P5056_01015"
                     /inference="COORDINATES: protein motif:HMM:NF014759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HRY62335.1"
                     /translation="MESHTLKKYPKRLVLLDVHAILHRAYHAIPDFTTSKGVPTGGLY
                     GLSMMLIKIIGELKPDYIAACYDLPEPTFRKKLYDAYKAGRKKADDALIDQIIRSREI
                     FEAFNIPIYEKGGFEADDMIGTIAETAKKEKDLQVVIASGDLDTTQLIEDDKVVVYTL
                     HKGIKDTIIYDEKGIEERFGFGPRFMADYKGLRGDPSDNIIGIKGIGEKTKIKTLVSY
                     SS"
BASE COUNT         4722 a         2976 c         3194 g         4917 t
ORIGIN      
        1 taaaaatatc ggaaaatttt ggtggaccaa aaaagaaaaa attacgaact atttgtccgt
       61 aattttgtct ttaaaattat taaaatggga ttatactgtc aatttagctc gtccctttct
      121 tctccttctc tggacaacct ttttgccccc tggagtggct tttctaacca aaaaaccgtg
      181 tgttttggct cgctttctct tttttggatt atatgtcttt gacattccaa gatattatac
      241 tgttttttat aaaattgcaa ggattattcg ctcaaagagc gaataatcct ttccatcacc
      301 ccaaccacga gcgagctgga gaaacccagg ccgaagacct tgcgcagaag gggtgggcat
      361 ggtctttgct gtagcaaagc catcccttcg agcagagcaa aatcgccgca gggcgattta
      421 aggcgtgtct tcggccgggg tttctccggt gagaagttat ccacacctaa tccacctatt
      481 ataaacagaa acgtctagtt cccttttcaa agtctcggca tataattatc tttgtaagat
      541 attaatcaat gactgatcac aaaaaaattt gggaagacgc tcttctagaa atagaaatgg
      601 gtgtctcgag ggcaaatttc agcacctggt ttaagaatac ttatatttca aaacaggaag
      661 acggcacgat ttacattaat gttcctagcg cttttgtaaa agactggctt caaaacaaat
      721 atcacaaact tatattaaaa gcacttcgag acactctgcc cgacacaagg ggagtggaat
      781 atattattca aaaagatatc caacctccaa aggagcatgc tggcgaacta gaacaaaaaa
      841 taccttcagc tcaactaagg cttaacgaac cttatgtaag tagagacgac aaccttaacc
      901 caagatatat ctttgatggt tttattgtcg gtccgttcaa tgaactagca tatgccgccg
      961 cacaagctgt aataaaaaat cccggagcaa catataaccc tctctatatt tatggaggga
     1021 ccggacttgg aaaaactcac ctaatccagg cgataggaaa ccatataaag aaaagcaaac
     1081 caaacgccaa ggtctattat ttagcctctg aaagatacgt tacggagatt gttaactcca
     1141 tcacgaacaa tacgataaat atcacaaagg aaaaatacaa aaaatacgac gctttgatta
     1201 ttgacgacgt tcagttcttc tcaaaaaaag ataaggttca agaagaaatg ttccatctgt
     1261 tcaacgattt ctacaataac aataaacaaa taatattttc ttccgataag ccgccaaaat
     1321 atattcaaga tcttgaagaa agattgaggt ccaggtttga aggggggatg atagtggata
     1381 tttcaaagcc ggaatacgaa tcgaagatgg ctattcttaa ccacaagctt aagaccctta
     1441 attattcttt gcccgcggaa atactggact ttatcgcttc tagcgtccag gagagcatta
     1501 gggagcttga gggtatatta aacgcggtta tttgtcagtc tcagctcaaa aataaggagc
     1561 tgaccgtcaa tgatgtaaaa actctcctaa aaaataattc aaaacaacaa aagagcttat
     1621 ctattaagga tatcgtcagg ataatcgcgg aattctacaa tatagaggag aagcatctat
     1681 acgaaaaaac aagaaaaagg gaggttgtta aacccagaca gatagcaatg tatatattga
     1741 gagaaaactt taattcttca tatcctcaaa taggggagaa gctcggaggc agagaccaca
     1801 caactgtcat acacgcctgc gaaaaaataa aagaagatat aaaaaagagc gacaatttaa
     1861 tgagagagat agaacaaatt aaatcattgc tcttcggtcc tgaaaataaa atatcttcta
     1921 gcgccggagt cgtttagttt tccacagacc gctactaaaa tataggcgac aactgttcaa
     1981 aagatgggga taagaggaag aaaagttgtg gtaattttgg gaaaaagtat ttatcctttt
     2041 ttgaccacag acttaatccc aaaattataa tttctgagtc acaaagtatt gcacaagaac
     2101 gaacttattt ttacgttaaa agcaaaagat aaaattcggt tattaacata tcaacagaac
     2161 taataatagt aataatttat ttatatataa aagaagtaag aattaagaat acagaattaa
     2221 gcacactcta aaaaagtaaa tgggggatac taaaaaaata aaatctttta cggacttgat
     2281 tgcttggaaa gaggggcata atttggtatt aaaaatttac gagttaacaa aattatttcc
     2341 taaagaagaa ttattcggtc ttgtgggtca aatgagaaga tgcgctgttt ctattacatc
     2401 aaatatagcc gagggattta gccgacattc atataacgat aaagcccatt tttattcaat
     2461 atctcttggt tcaatcacag aacttcaaaa ccagcttata attgctagag atatcaaata
     2521 tatagagaaa agtatttgca acgatgtata tgaacaatca ataaaagttc ataaaattat
     2581 taatggttta ataaagaatt ctaggattat tcataattcc taattcttta ttcataattc
     2641 ttattttaat gaaagccgag tgtataaaag acaaattaaa agaagctgta tttcaggtag
     2701 gaaagatctc tgctaagaat ttatcattac ctatactcga atcaatactt atcgaagcaa
     2761 aaggatcgga aataatttta aaagctacga atcttgatat cggattagaa gttaaagttc
     2821 ccgcaaaggt tgaaaaggcg ggatctgtcg cagttcccgg ctatattctt gccggtttta
     2881 tacaaatgct tgaaagcgac aatgttgtta aactagaggc tataaataac aatctttcgg
     2941 tatctacaaa aaagaactca acgcttatca aaagtttccc cgccgacgaa ttccctttga
     3001 ttcctagggt tgaaaaggaa gaatccctta cagttgaggc ttctaaaatc gtatccggaa
     3061 taagagccgt ttcttttgcc gctttaggct cggatataaa accggaaata tccagtgtgt
     3121 atatttatgt ggataacgga agtctctatt ttgtttcaac tgattcttcc cgattagccg
     3181 aaaagaaatt tgatgtaaaa gcggattccg acttctcttc ttctataatt cctattaaaa
     3241 acgctacaga gttggtgagg ataatggaaa atgttacagg caatgttctt ttagagttta
     3301 ataagaacca aatgtccgtg tctgcaccta atttttactt cacctcaagg gtcgtcgggg
     3361 gtattttccc ggattataga cagattattc ctaaagataa gaagactgag gtcgttgttc
     3421 ttaagcaaga gcttgttgat tctataaaat tgtcttcgat cttctctgat aaatttaatc
     3481 aggttacttt taaaataatt ccttctgata gtttctttga aataagctca aggaatcagg
     3541 acaaagggga aagcgtcact aaaatagagg ctactttaga aggggaggat attgatatga
     3601 gctttaattc cagatttatt ctcgactgcc tcaattctat taaagaggac agcgttagta
     3661 tcgcgtttaa cggccaccag aggcccgtaa tcatcaaggg aataggagat gagtcattta
     3721 cctatctcct aatgccgatg ataaaataga gggtagatgg atagtggaaa gtagttatta
     3781 tttttgtcat tcccgcgaag gcgggaatcc atcttttaaa tctacataga ttcccgcctt
     3841 cgcgggaatg acagcaaaga gacaatattc taaatatgca acaattatct tcacttttag
     3901 aacgatttaa gcgcttttct aggccaaata ttgagataag agaggctgtc tcaaaagcta
     3961 taaagagcga aataggagcc attgtctccg aaaaggatgt ttccatcaaa agcggtgtgg
     4021 catatgtaaa aacccacaat tctattctaa aaaacgaact cttcctcaaa aagaaaaaca
     4081 ttctggatgc tgtccagaat gtttgcggcc acaaaagtat aagtgatatt aagtaaaaga
     4141 ttattttttg tcattccgag cttgccgagg aatgacaggg gaggggcagt agtttttaat
     4201 tttgcgaatt tttattaaat agcgacctta aagatagagt tgttttctcc ggaatttctc
     4261 acagagtcgt aaataatcac tttaagattg ttcacgttct ttatttgtgg aatatcggaa
     4321 ggcacaaaat agaatgaaaa tggagcattt tttgaagaac cgataaatgt gtcatttata
     4381 aagaaatcgg cttgagtaat aggatattta ctcgggcttg aagtgaaaaa taaacctatt
     4441 ttttcgttaa gaggatattc tctgttctgg tccggagaaa caatatttat ttttggagca
     4501 aaatttggtc catgaatatc gtctgcggct gtaggaatta cgcttattga ttcttcctgt
     4561 attttttgct cggccgccca cctccttact gaagtttccc atgaattaaa ctgtggatct
     4621 tgctgtggat atataggcgc aggaccaaga gggtcttttt tgttcaacca ataaagtatc
     4681 gtgtgaacct gtgtaaccgt ttttatttct ttcatttccg gaggggtata ttccgtggat
     4741 aattttccgg aggctttgtc tattgtgtaa ggaaatcctc ctctccagtc tcctcttaag
     4801 acaggtttta gactgtcggt gttcatctcc gccggttttg tgaatttttc tgcaggtagc
     4861 gtgagtaaag ctttatcaaa gaaagctcgc cacatcgggg ctgtgatcat tcctgctacc
     4921 tttttcacca taggggtgtt gtcgttgttt ccgacccaga ctccaagagc aaagtttggc
     4981 gtatatccca cgacccaagc atctttcgtg tcgtttgtcg ttcctgtctt tgaagcaacc
     5041 tctctgttgt agaagtgcaa aggagaattt tctccgtaag cgggcagttt tgcattgaag
     5101 tcagaaagga cgttgtttat ttgtctggca acattttcat ccaacactct tactccattg
     5161 tccgaatatt cttcaagtat cgaaccggat gagtcaacta ctttgagtat cggtgttgcc
     5221 ggatttctta ttccatcatt tgcaaaaaca gaatatgcgc ttgttatatc aagaggggtg
     5281 acctctccgc cccccaaaac aagggttaag ccgtattgat ttgcgtccgt gaggccggta
     5341 ataccaaggt ctcttgctgt ttgtatagcg ttctcaatac cgactaaata taaaagtttt
     5401 atggccggaa tgtttataga ttgagccaga gcgtctctta gttttatcgg gcctctatat
     5461 atgccatcat agttttccgg catataacaa tcggcagggt ctacctcgga agatatagga
     5521 agaccttccg gtgtgcaggt agtttggaat tccgtaggaa gatcaaaaag aacagtgtcg
     5581 ggtatgtaac cttgtttaaa agcagtagcg taaacaaatg gtttgaaagc ggaaccgggt
     5641 tgcctgtgtg cgagagttac gttgaagttt ccgtcatttt cagtattaaa atagtctctc
     5701 gaacccacca ttactaatat ttgtccggtt ttcggatcaa tgccgatcat tccggcattt
     5761 tttgctccaa attgttcctc gtttattttt ccataatcgg acactacttg ttcggcttct
     5821 ttttgtagct cccaatcaag cgttgttgtt acatttagtc cccctgtttc tagaacctct
     5881 ttgccatatt tgttttcaag ataacttctc acatattcta cgaagtgggg cgcctttatg
     5941 cctccttttt cttcttttga gataaacacg acttgctcgt tttttgcctt ctccgcttct
     6001 tcttttgata tgaaattcaa ttcaaccatc ctgttcaaga ccagattttt tcgctcgtcc
     6061 aatttatcct tattctgtcc gtaaggggag tagaacgtcg gagcttgagg aagcgcggcc
     6121 aaataagcgg cttcggctag ggtgacatct tttgcagatt tgttaaaaaa tctttgagaa
     6181 gcttcttcta ctccgtaagt agtgcctcca taaggtgatt cgtttaaata aagagaaaga
     6241 atgtgttctt tatccattac tcgctctagt tttaaagcta aaatccattc ttttatcttt
     6301 cttgtatagc ttttttctga agttaaaatg gagtttttta taacctgctg agtgattgtg
     6361 gatcctccct gaaccttgct tccggaagtg agattaacaa aaacagccct caaaatagcc
     6421 atcggcttga taccgtagtg gttgtaaaaa tctgaatctt caatggcgac ggtggcgttt
     6481 tttatgtcct ttgaaatgtc ttcataagga acaacctttc tttttatatc ttgatgaata
     6541 tcataaagaa gaattttgcc tgttttatca aatatttttg ttgactgaga aatttttctg
     6601 gattcaaaag agccaaagtc ggggatttta agatttgcga tccagagtat tcctcctcct
     6661 gtcgctaaaa atagtaatat agcggatact attaagattc tttcgatttc tttcttaata
     6721 aatctaatta ccttagttgt ttttttcatt ataggacaac aataccatat ttattcgccc
     6781 ttaaccagtt tgaaaaattc cacgttttta atatatggct ctaaataagg atataagcgt
     6841 tttagtcttg acctcctacg gggcggagag tcgggtgtag ttaaaataga acaattacaa
     6901 gccacttagt agacgggttc ctaagtggct ttgttttggg ccatatattg gccatattaa
     6961 aacattgata aataagactg cttcggcaaa cgggggatat tttttttgtt gaaacaaata
     7021 tccataaaat ttgatcagca cttagttttt aatcccgccc gggtgtgata ttatctaaat
     7081 gttaaatata gataatggaa tatatggaaa ataaagagca aattaatgat gtaattactc
     7141 ggggggtaga aaatatttat ccttccgctg actttttgcg agctaaactt gagaggggag
     7201 aaaaactcac tatttatcta ggtattgatc cgaccggacc gacacttcat ttaggacacg
     7261 cgatagcttt gaaaaaatta agcgaatttc aggctcttgg atgtaaaata attatgctta
     7321 tcggtgattt taccgctatg ataggcgatc ctaccgacaa aaaggccaca agaaaacaat
     7381 taactcgaga agaggttttg aataactgta agttgtataa aaaacaagct tccgtatttc
     7441 ttaattttaa aggagataat cccgcagaac taaaatacaa ttcaaaatgg cttgcaaaga
     7501 tgaattttga agatgttctt aatttggctt caaaaatgac cgttagccaa atgctcgaaa
     7561 gagatatgtt cgagaacaga atgaaagaag gcaggccaat attccttcat gagtttatgt
     7621 atcctcttat gcagggttat gacagcgtgg ctatggatgt agacggagag gtgggaggga
     7681 atgatcagac atttaatatg cttgtcggca ggaatttgct gaaggaactt aaaaacaaag
     7741 agaaatttgt tctgacaatg aagcttctta ctgataattc cggtacaaag atgggcaaga
     7801 cagaggggaa tatgctctct cttatagacg atgcaaatat gatgtatggc aaggttatgg
     7861 catgggaaga ttctcttatg tggaatgctt ttgttctttg cacgaatgta gacgcggaag
     7921 aaatagagaa aatgaaagcg gaagtgtccg gagggaaaaa tcctagagat ataaaaatga
     7981 gacttgccta tgagattgca aggatctatt acggagatag taaggccaaa aaagctgaaa
     8041 aagaattcgt caaaacattc cagaagaagg agaccccgga tgagatggag gaaataaagg
     8101 taaatgtcgg agatttattg gctgatgttt tggtcgttcg cggttttgta aaatcaaaat
     8161 cagattttag aagacttgta gaagagggcg ctgtaacgat agacggagaa aaaatatcag
     8221 atttccagat caaaatagaa aaaacaaatg tggttctcaa gataggaaag aagaggttta
     8281 ttaaattgat ctaatctttt tatggcattc tcaccagaag gaagaagtgg agaaaccgga
     8341 ggaacacttg agtggggcgt ttttggcggc ataggaaaag gaaaggtcat gacagcgatc
     8401 aaagaaatgg gattgaccac ggaggaattt ttaaaattga aggataaagg atatttgcca
     8461 tacgataagt caatagatct cgtcaaaaga atccagaatg ttacagaaaa acacaacccg
     8521 gctaatcctg aaaaaatgat agttcgtgat ataaaagatt ttttggagga gtttttaaag
     8581 aagaagtctg aaaatgtgcg tttttattcc gcgctaaaaa caccgctcga tattatgcat
     8641 ggggtagatg ctttctttga aattgatctc gagaaagagg agggtaaaga acaacaaaag
     8701 cccgttgtgg tgacaatgga cattaccatg agggcaaaag aaggtgataa ggaccaaaaa
     8761 gctaatgttt tgatacgaat gcccgatggc gccccgcttt cggcagatga cgattacgaa
     8821 gcgaaggtaa aacaaatagc tagggcggtg aaagatgctt ttgaaaatat aaaatcaggc
     8881 gacaaatttt tcaaaacagc tattacaata taatttaaaa aacaccccga tggggtgttt
     8941 tttgttttaa cgtttgactc taagttcgac gctaaatata caaattattt tatattccgt
     9001 cgcttccgga gcctatatca agacccaagg ctgtctccgg atcaaagtcg tcatctgaag
     9061 ctgttatgcc ctcgagagcg tcttcgctaa gggcggcatc aaggttttca tcaaagcttt
     9121 cataaacctc cgctcctcct tcttcatttt gtgctttctt tggttttggc atttctttat
     9181 gcaaaaactt agtaaaattt ataatatata ctatcttttt ttattttatt aaagataaat
     9241 ctttttagca aagttcggat tattttgcaa ccctttctgt cgcaagacag gtgagcgcta
     9301 tcgcacaggc gtcaaattca tcatcttcta ttttattttt tttgaactca gaaaaatttt
     9361 ttataagaag cgggaccatt tttgagactt gatccttgtt tgcttttccg tatccgacca
     9421 tcgccatttt tacctgaagg ggagtgtatt cataagtctt aagtccgttt acttttgcta
     9481 tataagtagc gatacctttt atttcagaaa cccccatagc tgttttttgg ttcgttgtaa
     9541 aaaaaagttt ttctatggca agcgcatcag gcttaaattt ttctatcaga tgctgaaaat
     9601 cagatccgat tgtaaaaagt ctctccgaaa atggaagttt cgctgacgat ctaaggcaat
     9661 ctgaaaaaat aaggatatct tggccgtttc ttttttctaa gatagcgaca ccaaatttat
     9721 cgtagcccgg atctaccccg ataactctca tggtttttag atattaatga tgctgtcatt
     9781 cccgcggagg cgggaatcca tatttttaat tctcgagaca ctagattccc gcctccgcgg
     9841 gaatgacgga aactagagat tagaaatgtt tgtgtagata ttttctacat cttcatggtt
     9901 gtctatcttc tccagaaggt tctctaatgc ggttttgtcc gaatcgctta attccatcgg
     9961 catttttgcc acccactctc catctgcttt ttcgaatgcc cagattacag atccttcggc
    10021 ccccatagtc ccgccgttat ccgagataat gtgttttatc tccggtgtca ttctgttttt
    10081 gttgtcggtt attccttcta ttatgattgc cgtccctccc ggtccatacg cttcatatct
    10141 gacttcagtc atttcggctc ctcctgcgcc ggttcccttc gccactgcgc cgtcgatctt
    10201 atcattgggc atattcgctt ctcgcgcttt ttctatagcc tttcttaatg agggggagtt
    10261 tttgtcaccc tttgcgagcc ttgattcaac cgttatcttt tttgcgagca tcgcaaaaac
    10321 cttgcttttt tttgcatctt ccgcgccctt tttattcttt atttttgacc acttattatg
    10381 tccggacata gattaattat gaatagtgta ttctttattt ctttctatta gtgttttata
    10441 taattttatt atctttaaag caaattcgta attctttttc tgaataatat tatctttcat
    10501 tacaattcgt aattggtaat tcgtaatttt taattatcct gcggtggcgg gatattaagg
    10561 tgtttgtatg cctttggtgt agccccgcga cctcgcggag ttctctcgag gaagcctaat
    10621 tgaataagat aaggttcgtt gacctcttct attgtcccta cctcctctga aattgccgct
    10681 cctatcgtgc cgagtcctac cggtccgcct ccgaatttat ttattatcgt ttctattatt
    10741 tttctgtcta ttatatttaa tcccgcatgg tcaattccga gtagatccaa ggcctcaccg
    10801 acgctttttt tatccaattc ctgctgtttt acttgagcgt agtcgcggca cctctttaaa
    10861 agatgattgg ctattctagg cgtaaaacgg cttcttcttg agatctcttt tgcggcatct
    10921 ttatttatag aaactccgag gatctttgat gaacgctcta ttattctttc tatttcttcg
    10981 tcggtataaa actcaagacg gaaagttcca cccgaaaagc gcgaacgaag gggagaggat
    11041 agcaacgcta tttttgtggt agccgcgatt agcgtaaatg gaggaagctc aagctgtatg
    11101 gttctggcgg aagggccttt gcctatgatt atgttcagca ttccggactc catcgcagga
    11161 taaagcacct cctcaatgag cttgttgagc ctatgtatct catctataaa taaaatatcg
    11221 ccggccgaga ggtttgtaag gagagatgcg aggtctccga ctttttctat cgcaggaccg
    11281 gaagttatct ttatctgaga attgttctct ttggaaataa ggtgggccaa ggtcgtcttt
    11341 ccgagacccg gagggccata gaacagcagg tgttcgggag gctgttttct ctctttggcg
    11401 gcagacaaaa gaatcttcaa attttcctta attttctctt gtcctacata gtcatcccac
    11461 ctatccggac ggagggtttt atcaagaaaa acctcgtttt tttgggatga ggtgtcttct
    11521 agttccatat cgtttggaag gggggcactt gacatatttt ttcttttatg ctagacttgt
    11581 tttagttgag ttaagtttat ttacaaagga agttatgtat ggtatcaatt gtctttggcg
    11641 cgacaaacgc gaccttgaga caattataga ggatttagct ctccaagcaa taaaacttac
    11701 gggtttaaat ggttttttgc ggaggacgcc ctggtcttca ccttagagtg aaatcgctgg
    11761 gaccatcctt caaaaaaatc taacttttga tgaatcatca aaagaattgc ttagagaatt
    11821 aaatgctcta tgaaaataac tacttagttt atactgagtg gttatttttt tatttatcta
    11881 taaaagaacc aactagcgaa aatctaaaaa cgaaaagcgt aaaataacaa cttaaaattt
    11941 tgaattaatc atctttttta ttgttttgca tttttcgttt tactctttgc gtttcctaat
    12001 acagctcttc cgccaacctt agctctacca ctctctccaa ctcttctatc gaggtcattc
    12061 cctgtaaaac tttaagtatc ccgtgctctc tcatgtccaa tattccttgt ttgtgtgatg
    12121 cgatctttat gtccctggcg ttcacttctc cggtaataac tcggctgacg tccgcgtcta
    12181 cgataatggc ttcaaatact ccggttctgc ctttgtagcc gacaccatta caaaattcac
    12241 agccttctcc ggctacccag acttgcttac cgtccggaac agcgatatcc ggtctctttt
    12301 ctttgatcaa tttggaaaca ttttctatca tttttgcgtc ttcctctttc ggggtagtcg
    12361 cttttttgca ctccgggcat aattttctta ccaatctttg ggctatggtt aacgtaaggg
    12421 ccgagctgat aatctttggg ttaacaccaa gatctaccaa tctaggtatg gcaccggcgg
    12481 cgtcgtttgt gtgaagggtg gagaatacca agtgtcccgt aagagcggaa tttatggcaa
    12541 ttttggctgt ttcggggtct ctgatttctc ccaccattat aatatcaggg tcttgtctaa
    12601 gagcggacct gagtccttcc aagaatgtat aaccggaatc ttcgctaact tgtgtctgtg
    12661 aaagaccctt catgtgatat tcaacaggat cctctattgt gatgattttt gtttcagaat
    12721 cgttaacttt tctcaaaaag gcgtaaaggg ttgtcgtttt tccggaaccg gtaggtcctg
    12781 tcgtgagaat aaggccgttt ggctttttta tttcatccaa aaatacttta aggagctttt
    12841 cctctattcc catatcttca agatttacag agatggagtt tggatcaaga agcctcataa
    12901 cgattgtctc accataaaca cccggcaaga cagaggttct aacctcgata tctctatctt
    12961 ccgcgacaat cgtgaatctt ccgtcctgag agctcttctt tacattaagc ttcagcgcgg
    13021 aaatgagttt gattcttgat acgatgagtt tgtagacatt atgatcgaag aaatcaacat
    13081 cgtggagcat tccgtctagt ctgtatctga gccttacgaa ctcttcttga ggctcaaggt
    13141 gaatatctga agcttttgtt gcaagcgctc cggcgataac gatctcaagt attctagaaa
    13201 caccttggct tttgttgccg gcggtaagtt cctttatctg atttttaaca tcagcaacgg
    13261 cttttgtctt cgcaaggact tccgccagaa gttccggaga gatatctacg aggccggcct
    13321 tggtctcaac cgcctgcgag atctctttgt atcttcccca cgctctttct aggcttcttt
    13381 ctgaacccat atataacaca ggggagtacc ctcgttcctt aaggtcgttt aatgcctcat
    13441 caagtgcggg attgttcggg gaaaagacca aaatgctgac ttttttgccc gtcatctgaa
    13501 agcccgctat tttagcctct ttggccacgc tttcttctat gagcctgagg gcgtctgtgt
    13561 ttatagaaac cgtgctgagg tcaagataag gaatgccgta ttttccggaa agtatctgag
    13621 cgagatcttc cgcttcgcgg atcttcgtct cctccagttt ctttaagctt tgtgtgtcgt
    13681 caaatgttgg catttacccc gtgaaattaa attaatatta cctcgaagac ctagcgatgc
    13741 tactttaatt tattttttat accccatcta aaccgtctcc tatttctccg gggttctttt
    13801 cgggttctta tacgcttatt ctactttttt caatggtagt tacaaatttt ttatcaacag
    13861 agtataaaaa atttctttgt gcaacacctt ttttaatggt aatatcattt ttgtgaaaaa
    13921 aataacgaaa aatttacagg aaacacacga cgcggcgcga gatttcgcgg cctctttaag
    13981 catggctgtc gataaggagg gggagagtga tggcgcgata gttgtagcac tttccggcga
    14041 cctcggttcc ggaaaaacat cattcgttca gggtgtcgcc aaatatttcg gcattaaaga
    14101 caagatcgtg agcccgactt tcgttataca gaagagatat gaacttaaag gacataaaaa
    14161 tttgaagaat ttggtccaca tagatgctta ccgcctcctg aagccggaag aaataacaag
    14221 gattggctgg aataatctta taaaagagaa aggaaatatt gtttttgtgg aatggccgga
    14281 gaaaatagaa ccgcttttgc cgaaaggcca caaaaaactc gtttttgaat tcatcgaccc
    14341 ggaaaagagg gggatagaag gggtttaagg cccgaaagtc cgtagtcaaa taaaggttta
    14401 aatttggtca ttgcgagcaa ggtgaagcaa tccggattta agatgaataa tccctagatt
    14461 gtcgcgctcc gctcgcaatg actggtatac ggggaatatt ctataatttg acatcctata
    14521 taccaaagta tatactttgg taatgaatac aaaaacactc ataaatatta aaacggacaa
    14581 aacgatcaaa gaaaaagcct ctaaagttgc ggcggaactg ggtatgccgc taggcactat
    14641 aatcaatgct tttctccgtc agttcattat ggataaagag atccatattt cagcttcata
    14701 taggccgtct cccatgttaa agacaattat tgccgaggcg gagatggaca tcgcggaagg
    14761 gaaggggctt tctcctgtgt ttagtaaagc agaggatgct cttaaatatt tgcgatcata
    14821 gtgaatattt atgcgaatcc aatttcacaa aaggttcatt aaacagcaca agaatctcaa
    14881 aaagatatgg gataagtttg atgaaagatt tattctcttt acaaaagacc cgaatcatcc
    14941 attactcaat aatcaccctc tccacggtaa atacgataat tacagaagta taaacattac
    15001 cggagatatc agggctgtct ataaagaagt taataaagat cttgttattt ttatcgctct
    15061 aggtactcat agcgagctgt attcttagtt ttttattaca aaatttaggc gggtgaattt
    15121 tttagtaata aagtatagaa tactagctat ggaatctcac acattaaaaa aatatccgaa
    15181 aagattagtg cttctcgatg tgcatgcgat acttcatcgc gcctatcacg ccattccgga
    15241 ttttacgact tcgaaagggg ttccgacagg aggtctttat ggcctctcaa tgatgcttat
    15301 taaaataatc ggtgaattga aacccgatta tatcgccgct tgttacgatc ttcctgagcc
    15361 gacattcaga aagaaacttt atgatgcata taaggcgggg agaaagaagg cggatgacgc
    15421 gctcatagac caaataattc gctcaagaga aatttttgaa gcgttcaata ttcctatata
    15481 tgaaaagggg ggatttgagg cggacgatat gataggcacc atcgccgaga ctgcaaaaaa
    15541 ggaaaaagat cttcaggtgg ttatcgcctc gggagatttg gatacaactc agctcataga
    15601 agacgataag gttgttgttt atacccttca caaaggtata aaagacacca ttatttatga
    15661 tgaaaagggg attgaggaaa gattcggctt tggtccacgg tttatggctg attacaaggg
    15721 ccttagagga gatccttcag acaatattat aggaataaag gggataggtg aaaaaactaa
    15781 aataaaaacg ctggtctctt attcatcgg
//
LOCUS       DAOTEX010000009        15323 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3118
            NODE_2644_length_15323_cov_4.454873, whole genome shotgun sequence.
ACCESSION   DAOTEX010000009 DAOTEX010000000
VERSION     DAOTEX010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868142
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 15323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 883
            CDSs (total)                      :: 833
            Genes (coding)                    :: 829
            CDSs (with protein)               :: 829
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15323
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2644_length_15323_cov_4.454873"
                     /isolate="AMR_MDS_3118"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(43..1911)
                     /gene="asnB"
                     /locus_tag="P5056_01020"
     CDS             complement(43..1911)
                     /gene="asnB"
                     /locus_tag="P5056_01020"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HRY62336.1"
                     /translation="MCGITGFIGKGTKSDLVKMVAAIEHRGPDAQGHFWSEGVALGHT
                     RLSIIDLSETGAQPMFDNNKEIGVVFNGEIYNFPELRKELEVLGYVFRGTSDTEAIIY
                     LYKEYGEKCFERMNGMFAIAIYDFKNKKLILARDRMGKKPLYWALFEKTLIFGSELKS
                     LIPHPLFKKELDLNSVNKYLQYDYVPSPHSIFKNVYKLEPASCLVFENGKINKNIFWG
                     ADLTPKDISLNEALEELDSNINSSVISRLVADVPLGIFLSGGLDSSAIAYYAQKNSKD
                     KIKTFSIGFREDSFDESKYARQVAEYLGTDHYEKILSAKDSLSMIPKIADILDEPVAD
                     ASIIPTYLLSKFTKEKVTVALGGDGGDELFAGYPTFQADSLVGLYEGIPEIVRKEMIE
                     KIIKSIPASEKNFSLGFKLKKFISGLGVNKKYLHQTWLGSFGREDRGQLFKERVWKNL
                     SSENEFEDIDRYLDEVKDGDHRNKTLYLYMRTYLMDQVLVKVDRASMSSAMEVRAPFL
                     DISIVEFANSLPYRYKYRNFQTKYILKKLMTGKLPKNIIHRSKKGFGIPLGKWLKEDL
                     KDFCNKTLSRENSDKFGLFNYEYINKIKNEHFSGRKDNRKLLWNLMVLFLWREKWF"
     gene            complement(1917..2861)
                     /locus_tag="P5056_01025"
     CDS             complement(1917..2861)
                     /locus_tag="P5056_01025"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004512828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRY62337.1"
                     /translation="MKISIVVPVYNEAKSLPELFLEIKEVLEKERSDYEIVAVNDGSK
                     DDSWNVLKKLGEDNKRIKAINFKFNNGQTAAMSAGIKASTGDVIIPIDSDLENDPADI
                     PKLLAKISEGYTVVSGWRKDRWGNATLKRKIPSLVANWLISKITGVKLHDYGCTLKAY
                     DRGIISEVELYGEMHRFIPAYAKWKGGKVAEIPVNYRPRKYGESNYGISRTFRVLLDL
                     VLIKFLVKYMNRPMHFFGGLGFSSVLLGFLSGCYALILKFVYGTSFISTPMPVLTVFF
                     IIVGVQLAAMGILSEMIMRTYYESQNKTPYSIKEKINF"
     gene            complement(3294..4202)
                     /locus_tag="P5056_01030"
     CDS             complement(3294..4202)
                     /locus_tag="P5056_01030"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRY62338.1"
                     /translation="MIDYGKPGGLIQYFIFAVKVLFYCLKSDSDIFYSRDEMPLWFLS
                     IFNKKVFWEVHTGKWNFLVSDTVKRCHGIITISNGIKKYFENKNIDGDKILVSPDAVD
                     IEQFSLDIKKNDARKKIGLPSDKKIILYTGHLYAWKGADTLANSAKEFNEEYLFVFVG
                     GNINHVNDLKKRFSNQDNIMILGQKPHEDIPFYLKSADVLVIPNSGKEDVSRLYTSPM
                     KLFEYMASGTPIIASNLPSIREIIDEDGAVFFEPDNPESLARSIKKIFDNSALADKMA
                     NNSKINSQKYSWQRRAEGIINFISSR"
     gene            complement(4214..4405)
                     /locus_tag="P5056_01035"
     CDS             complement(4214..4405)
                     /locus_tag="P5056_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62339.1"
                     /translation="MKISYLANIRIPTDRAHGIQIMKTCEALSSQGVVVELVVPSRKN
                     SIKEDPFSYYQVKENFKIP"
     gene            complement(4402..5085)
                     /locus_tag="P5056_01040"
     CDS             complement(4402..5085)
                     /locus_tag="P5056_01040"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRY62340.1"
                     /translation="MNKTQEQRFNKVKESVIFKRIRSKYSLSEKRVLDIGCGYGEYLL
                     FFGKGSVGLTTDEEEIKFGKDNKLNIKFGNAEFLEESLSGDEGFEAIWANNLFEHLLS
                     PHSFLMKLKNSSEGDTKLILGVPVIPSIYFLTGINKFKGAFASNHIGFYTRMTLRLTI
                     ERAGWVVEDIRPFIFYNKYLDFLASFLSPHLYVIARNNTNFIYPSKKFNEWFADKHYD
                     FLFKITGQK"
     gene            complement(5194..6249)
                     /locus_tag="P5056_01045"
     CDS             complement(5194..6249)
                     /locus_tag="P5056_01045"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRY62341.1"
                     /translation="MKLLIITQKIDKQDPVLGFFHRWIEEFSKHCEKLTVICLEKGEN
                     DFSEKAEPCSENSKEFSTTRVKVLSLGKEQGVSRFEYLKRFYTYIREEQDNYDSVFVH
                     MNQEYVLLGALPWKTWGKKVFMWRNHPNGSILTDLAVYFSDRVFCTSSYSYTARFKKT
                     EIMPVGIDTEFFKRDEEIRKKDNSILFLGRISPIKKPLLFIEALNILNKKNVDFNASI
                     IGDPLPKDNKYFEEVKGKVSQYGLVNKVTFLKSVTNTEAKSIYNSHKIYVNLTPTGSM
                     DKTIFEAMACDSLVLISNKSLFGKVEDRFLCREGDAEDLAKKLAIMIKEKDDSSDLRK
                     YIENNHSLKKLAEVLLK"
     gene            complement(6454..8109)
                     /gene="groL"
                     /locus_tag="P5056_01050"
     CDS             complement(6454..8109)
                     /gene="groL"
                     /locus_tag="P5056_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255396.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HRY62342.1"
                     /translation="MAKQIIFNNEARQALRHGVDKVANAVKITIGPKGKNAVIDKGYG
                     APIITNDGVSIAKEIVLGDKIENMGAEIVKDVAGKANDMAGDGTTTAVVLTQAIIDEG
                     FKRIALGVNAMGVRTGIEYATKAVVEELVKMAKPIKNRNEIVQVATISAESSEFGEII
                     TDAIDKVGKDGVVTVEESQGFGVESEVVEGMQFEKGYVSMYMVTNTERMEAEYEDAMV
                     LLTDKKISSIKEILPLLEKMAGMGRKELVIIAEDVDGDALSTLVVNKIRGSFSALAVK
                     APGFGERRKEMLEDIAVMTGGKVISEDVGLKLENATMDMLGRARKVVSTKDMTTIVGG
                     KGKKANIEARVAQIKTQIAATESSFDKEKLQERLGKLSGGVAVIKVGAATEADMKYKK
                     LKIEDAVEATKAAIEEGVVAGGGTALIRAMARVADKGVKSPSEDIQNEFQAGVSVLLS
                     SLSSPLRQIAINSGKDDGLVIVDKIKSSKDNEGYDANKDKIVKDMISAGIIDPVKVTR
                     TALEHAASAAAILLTTEVAIAEEPKREESQGGGMGHSHDEMGY"
     gene            complement(8214..8525)
                     /locus_tag="P5056_01055"
     CDS             complement(8214..8525)
                     /locus_tag="P5056_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013735873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HRY62343.1"
                     /translation="MSKKIAIQPLHDRVVVEAIGENERGKETKSGIFIPETVDKERAE
                     RGTVVAVGPGKMNDDGKRIPMSVKVGDKIIFAKYGPDEIKVDGKEYFILSESNILAIV
                     E"
     gene            8668..8895
                     /gene="secG"
                     /locus_tag="P5056_01060"
     CDS             8668..8895
                     /gene="secG"
                     /locus_tag="P5056_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HRY62344.1"
                     /translation="METIITFLPWIQIALSALLIGVILIQQSEAGISGNMGGGQHRTK
                     RGLEKGIFIGTIVIAILFAASALLAIAYSSF"
     gene            8950..10767
                     /locus_tag="P5056_01065"
     CDS             8950..10767
                     /locus_tag="P5056_01065"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRY62345.1"
                     /translation="MKEKISILLKKEFNIPIPGFLRHEKLKFIFQDLPKKDELLVATK
                     TFSKKERLFFWTFAFLFCSTGAFLLWDINKDLMIERPVKGGEIVEGVADMPRFINPLL
                     ATGMADADITSLVYSGLLRPGPNGEYINDLAESYTVSKDGLVYNFKIRENATWHDGKP
                     VTADDVLFTIQKAKDVVLRSPKRASWEGVTALVISEKEIQMTLKQPFQSFLENATMGI
                     LPKHKWNNIDSDKFALSYLNMRAIGSGPYKVKTISKRTTDDMPEYIELIPFREFALGE
                     PKISKITIHFYQNQEELYTAYKKGTVTSMSSPSPKFVEELKNSKTNIIQSSLPRIYAI
                     FFNPNESKLLADKEVRRALDMAIDKKGIIDNVLLGYGTIIKGPIPPDSLGFTETNNAE
                     ASTTPATKIAEAQKILAKLGWKKNEKTGILEKKISKKETQVFEVSISTTDFTELKTAA
                     GMVKADWEKLGAKVNLQIFEKGDLDRNVIEPRKYDSLFFGEIVGRDLDLFSFWHSSQK
                     KNPGVNVSMYSNTKVDKILESLKAERDNAKKIKLLKNFQTEIDKDIPASFIYSPNFIY
                     LLPKEVKNVNILPPVIASDRFLNVYEWYINTEKIWKIFQ"
     gene            10880..12793
                     /locus_tag="P5056_01070"
     CDS             10880..12793
                     /locus_tag="P5056_01070"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HRY62346.1"
                     /translation="MINFHGRNGGASSSPNRARRPAPRRSPERGFVSGTKRFGQKPNE
                     ISASGPIKKSFRQTSQGHRSYQKKNMVEPKATPKAYDDTIRIIPLGGVEEIGKNMTVV
                     EYKGDIVIVDMGFQFPGEEAPGVDYIIPDTTYLNNKKKNIKGVFITHGHLDHIGGIPY
                     IMDRIGNPTIYTRLLTSLLIKKRQDEFPHFEPLNLRIVETAKTMRISDNMTIRFFNVT
                     HTIPDSMGVIIETPHGNIVFTGDIKVDHENGEPLPHEVETFTKLGNENNLVLLSDSTN
                     VEKPGWSFSEKMVHENLKNIIRDVKGRLIIGTFSSLLERIMFIIKTSEEMGKKVVIRG
                     KSMKNNIAIAMEMKLLKINPTTIIPPEEIDNYPVDKIVVLATGAQGDEMAALMRMSQK
                     KDKYIKLNKSDVVLLSSSIIPGNEKSVQKLKDNLSRQGATIIHYRIADVHSSGHANYD
                     ETLWVHKMIKPKYFIPIHGHHFMLRVHGEIAKIAGTPEANIVIPDNGMIIEIGDKGNS
                     IKVSKESAPAGVVMVDGLGNGDVSDVVIRDRQILAEDGMFVIIAVIDMKTGKIRQSPD
                     IISRGFIYLKESQDLLKDVRGLAKRTIESLAVDMHPINLDYVKNNLREKIGKYLMQNT
                     GKRPIILPVLLEL"
     gene            12852..14054
                     /locus_tag="P5056_01075"
     CDS             12852..14054
                     /locus_tag="P5056_01075"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRY62347.1"
                     /translation="MTLKEKRWMLFFIYFTTFLTSIGYAFIIYINSSFLQKFVSSEYI
                     GALYGVSSLVTLIILTRISYALKKIGHFKVMLGIITLETLSVFCSAILDMYSFSGITT
                     DYLRLSLFVILAFLIFNTTAILLRFSLDIYLEKYSKNRETGKARGIFLTTMNLAIAVS
                     PYMVGKILTNGDFWRVYLVASAIFLPAIIITGTKLRKVRDVEYHDTKFTIGLKKVWKN
                     KNIRSIFMTNFLLEFFYSWMTIYTPIYLFKIMGFSWENIGTMFSLMLTSFVILQLPLG
                     KIADKYLGEKEILTAGLIITGLSTSYLAFIEKPEFIVWTAALFMTRVGAAAIEIMNET
                     YFFKKVSPEDSDVIGFFRNASPLAYIIGPMTASVLIYFIDFKYIFAILGVIMLVGIRY
                     SLAIKDTL"
     gene            complement(14051..>15323)
                     /locus_tag="P5056_01080"
     CDS             complement(14051..>15323)
                     /locus_tag="P5056_01080"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HRY62348.1"
                     /translation="MDFLEAAKGRRLLRSAGGERSGMDAVHLMTAHRSKGLEFDHVYI
                     LGLYDGHWGNRRTKKHFKLPLGASTDGGNDDERRLFYVALTRAKKDIFLSYSKKDKEG
                     KLLLPSQFIEEIDPQFLDLKDEVSSPELAKDVLVKSFEPKVNRGTSADDKKFLNEIFL
                     SQGFSVTALNNYLECPWSYFYRNLIRIPEAPDKFLMFGNAIHYALKDFFDKLKEDDDI
                     GVQKLLQIFEKYLKEEPLSDEDLKETLKRGKEALRGYYKKYIKDWNIEILNEFKVHIV
                     LPGIPVTLTGKIDKIEILDAANNVNVVDYKTGKIKSRNEIEGKTKNSDGDMKRQLLFY
                     RLLLDNYDNAKYHAVTGEIDFIMPDPKGAYRKERIDINSEDVSGLRELIKKVSEEILN
                     LDFWDRRCEDEKCKYCSLRNSLNKKCRLGSA"
BASE COUNT         4687 a         3370 c         2638 g         4628 t
ORIGIN      
        1 actcatatcc gaatctttcg cttgatgaca ttttttaaaa aattaaaacc acttttctct
       61 ccacaaaaac aataccatta aattccacag aagcttcctg ttgtcttttc ggcccgaaaa
      121 gtgttcgttc ttaattttat taatatattc ataattaaac aatccaaact tatcagaatt
      181 ctccctagac agcgttttat tacaaaaatc tttcagatct tccttcaacc acttaccaag
      241 cgggatacca aaaccttttt tgctcctgtg tataatattt ttcggaagtt tacctgtcat
      301 tagtttcttt aatatatatt tggtctggaa attcctatac ttatatctat agggcagtga
      361 attcgcaaac tccacaatac tgatatcaag aaatggcgcc ctaacctcca tggcgctaga
      421 catgctggct ctatcaacct taacaagcac ctgatccata aggtaagtac gcatatataa
      481 atatagtgtt ttgtttctat gatcaccatc tttcacttcg tccagatacc tatctatatc
      541 ctcaaattca ttttcgctac ttaaattttt ccaaacccgc tctttaaaaa gctgacccct
      601 atcctctctt ccaaagctcc caagccaggt ctggtgtaaa tattttttat tcacgcccaa
      661 gccgctaata aattttttta acttgaaacc gagactaaaa ttcttctcag atgccggtat
      721 gctttttatt attttttcaa tcatctcttt tcggactatc tcaggtatgc cctcgtaaag
      781 accgacgagc gaatcagctt gaaatgtcgg atatccggca aaaagctcat cccctccatc
      841 tccaccaagc gcaaccgtga ctttctcttt ggtaaatttt gacaataaat atgttggtat
      901 tatagaagca tcagcgacgg gctcatctaa gatatcggca atttttggaa tcatagaaag
      961 tgagtctttc gcacttaata ttttttcata gtgatccgtg cccaaatatt ccgcaacctg
     1021 tctcgcatat ttagactcat caaaactatc ttccctaaaa cctattgaaa aagtttttat
     1081 cttatctttt gaatttttct gagcataata agctatcgct gagctatcta atcctccgct
     1141 caaaaatata ccaagtggaa catcggcaac aagcctagaa ataacactac tattaatatt
     1201 gctatccaat tcctctagtg cttcattaag tgaaatgtct ttgggggtta aatccgcccc
     1261 ccaaaagata tttttattta tttttccatt ctcaaaaaca agacagctcg caggctctaa
     1321 tttgtaaaca ttcttaaaaa tagaatgggg agacggaaca taatcatact gaagatattt
     1381 atttacagaa ttaagatcca actccttctt aaataaagga tggggtatta aagatttaag
     1441 ctcagaacca aaaattaatg tcttctcaaa caatgcccaa tacaaaggct tcttgcccat
     1501 tctgtctctg gcaagtatca gttttttatt tttaaaatca tatatggcga tagcgaacat
     1561 cccgttcatt ctttcaaaac acttctcacc gtattcttta tacagataaa ttattgcctc
     1621 agtgtcggat gttcctctaa aaacgtaacc caacacctcg agctctttcc ttaactcagg
     1681 gaaattatat atttctccat taaaaaccac ccctatctct ttattattat caaacatcgg
     1741 ctgtgctcct gtctcagata aatctataat gctgagcctt gtgtgtccca atgccacacc
     1801 ttcactccaa aaatgaccct gtgcatcagg gcctctgtgc tctattgcgg caaccatctt
     1861 aaccagatcg ctttttgttc ccttacctat aaatccggtt attccgcaca tttattttaa
     1921 aagttaattt tttccttaat ggaatatggg gtcttatttt gcgattcgta ataagttcgc
     1981 attatcattt cagagagaat acccatcgcg gcaagctgaa cgccgacaat aataaagaac
     2041 acagtaagaa ccggcatcgg cgtagaaata aaactggtcc cataaacaaa ctttaaaatc
     2101 aacgcgtagc aaccagaaag aaaacccaag agtacactcg aaaaaccgag tcctccaaag
     2161 aagtgcattg gtctgttcat atatttaaca agaaacttta tcaaaacaag atccagaagc
     2221 accctgaaag ttcttgatat gccgtaattg ctctctccat attttctcgg cctataattt
     2281 accggtattt ccgcgacttt gccgcccttc cattttgcat aagcgggaat aaatctatgc
     2341 atttctccat aaagctccac ctcggaaata atgcctctat cgtaggcctt gagcgtgcaa
     2401 ccataatcat gaagcttcac tccggtaatc tttgagataa gccagtttgc cacaagtgag
     2461 ggaatttttc tttttagagt agcatttccc cacctatcct ttctccatcc ggaaacgact
     2521 gtatatcctt cgctaatctt tgctaaaagt tttggtatat cagcaggatc attctcaaga
     2581 tcagaatcta tcggaataat tacgtcaccg gttgatgcct tgatgccggc cgacatcgcg
     2641 gcggtctgcc cgttattaaa tttaaaattt atcgccttta ttctcttatt gtcctctccc
     2701 aatttcttca gaacattcca gctatcatct ttagaaccgt cgttaaccgc aactatctca
     2761 taatccgatc tctctttttc caacacttct tttatctcta aaaatagttc cggtaaactt
     2821 ttcgcctcat tatatacagg aacaacaatt gatattttca tgggagggtt aaataaaaat
     2881 aaaatattta tacataataa aattaaataa aaatagcatg atggttatta tcgcttgaga
     2941 taataaatac caaaccccaa atatactcac aaacagatac ataaaagcac cattaacgat
     3001 caagttcaag agggaattta aaaaaaactt aaacatctgt aagtgtaccc gaccgactgt
     3061 ttgttcaaaa aaagtccaat acttctgtaa gaaaaagtta gcaaaaaaag tgaaaataaa
     3121 ggagatgttg gccgataccc aataattaaa cgacagcacg tcagtaaaaa caaacaataa
     3181 ggaaagctgt agcgccgcac acacgcaacc gacaacaaaa ctttttacaa acattctgtt
     3241 tttgtatatg tcggaaattt tattttttat attattcatt atgtctttca tatttatcgt
     3301 gaagaaataa aattaatgat tccctcggcc ctcctctgcc aggaatattt ttgggagttt
     3361 atttttgaat tattagccat tttatcagcc agcgcggaat tatcaaatat ttttttaatt
     3421 gatctagcta aactctctgg gttgtccggc tcaaaaaata ccgctccatc ttcatctatt
     3481 atctcccgaa tagaaggtag attagacgca atgattgggg ttccgctcgc catatattca
     3541 aaaagcttca tgggggatgt gtataaccta gagacatcct ccttcccgct atttgggata
     3601 acaagaacat ctgcgctctt gaggtaaaaa ggaatatctt cgtgtggttt ttgtcccaat
     3661 atcataatgt tgtcttgatt tgaaaatctt ttcttaagat cgttaacatg atttatgttc
     3721 cctcccacaa aaacgaagag gtattcttca ttaaattctt tcgctgagtt ggctaacgta
     3781 tcggcccctt tccatgcata taaatgaccc gtatataaaa ttattttttt atctgaagga
     3841 agacctattt tttttctagc atcattcttt ttaatatcta aagagaattg ttctatgtcc
     3901 acagcgtcgg gagatactaa aattttatct ccatcaatat tcttattttc aaaatatttt
     3961 tttataccgt ttgatatcgt aataataccg tggcaccttt taactgtgtc tgatactaaa
     4021 aaattccact tgcctgtatg cacttcccaa aaaacttttt tattaaaaat gctcaaaaac
     4081 caaagaggca tttcatctct tgaataaaaa atatcgctat cgctttttaa gcaataaaat
     4141 aaaactttaa cagcaaaaat aaaatattga ataagccctc ccggctttcc ataatcaatc
     4201 aaatcgggac aatttaaggt attttaaaat tttccttaac ttgatagtaa gaaaacgggt
     4261 cttcttttat actatttttt ctagatggaa caacgagctc tacaacaact ccttgcgaag
     4321 aaagagcctc gcaggtcttc attatctgta ttccatgagc cctgtctgtt ggtattctta
     4381 tgtttgctaa ataggaaatc ttcatttttg accggttatt ttaaaaagaa agtcatagtg
     4441 tttatctgca aaccactcgt taaacttttt actcggataa ataaaatttg tattatttct
     4501 agcaataaca taaagatgcg gagacaaaaa agaagctaaa aaatctaaat atttattata
     4561 aaaaataaag ggacggatat cttctactac ccagcccgcc ctttctattg taagccttaa
     4621 tgtcattcta gtataaaagc ctatgtgatt actggcgaaa gcgcctttga acttatttat
     4681 tcctgtcaaa aaataaattg aaggaataac aggtactccc aagattaatt ttgtatcacc
     4741 ctctgagcta ttttttaatt tcatcaaaaa agaatgggga gacagaaggt gttcaaaaag
     4801 attattggcc caaatcgcct caaagccttc atcaccggag agactttctt ccaaaaattc
     4861 agcattgcca aattttatat ttagcttatt atctttaccg aattttattt cctcttcgtc
     4921 tgtggttaaa ccaacgcttc cctttccaaa aaacaacaaa tattcaccat aaccgcagcc
     4981 aatatcaaga accctttttt ctgacaaaga atacttgctc cgtattcttt taaaaattac
     5041 agattctttt accttattaa atctttgttc ttgagtttta ttcataaaaa cttcaccgtg
     5101 ccttaactaa aaatgtaatg gcgctcacct aaaataaatt atattctttc ttttgtttta
     5161 gagctgtgac ctttttgttt ttatcaatca tgtttatttt agaagaacct ccgctaattt
     5221 tttaagactg tgattgtttt ctatatattt tcttaagtcc gaagaatcat ccttttcttt
     5281 tatcatgatt gccaattttt tagccaaatc ttccgcatca ccctccctac acaaaaatct
     5341 gtcttctact tttccgaaaa gactcttatt tgaaatcaaa accaagctat cacaggccat
     5401 agcctcaaaa atagtcttat ccatgcttcc ggttggggtt aaattaacgt atattttatg
     5461 actattataa atactcttgg cctcggtatt tgtaacactt tttaaaaaag taaccttatt
     5521 tactaagcca tattgactca ccttaccctt cacttcttca aaatatttgt tgtcttttgg
     5581 taatgggtca ccaataattg acgcattaaa atctacattt tttttattta aaatattcaa
     5641 tgcttctatg aaaagaagcg gttttttgat cggagaaatt cttcccaaaa acaaaatgga
     5701 attgtccttc tttcttattt cttcgtctct tttaaagaat tcggtatcta tcccgacggg
     5761 cattatttcc gttttcttga aacgagcggt atatgaataa gaggaagtgc aaaaaaccct
     5821 gtcagaaaaa tagacagcaa ggtcggttaa aatagaacca ttcggatggt ttctccacat
     5881 aaaaactttc ttgccccaag ttttccacgg caatgctccc aagagaacat actcctgatt
     5941 catgtgcaca aaaacagaat cgtaattatc ctgctcctct ctgatatatg tataaaatct
     6001 ctttagatat tcaaaacggg atacgccttg ttcttttccg agagacaaga ccttcaccct
     6061 ggtagtagaa aattctttag aattttcact acagggctct gccttttccg agaaatcatt
     6121 ttcacccttt tcaagacata ttaccgtcag cttttcgcag tgtttagaaa attcctctat
     6181 ccagcgatgg aagaacccca aaacagggtc ttgcttatct attttctggg ttattattag
     6241 cagtttcatt ttttcgtatt ttgagctacc ttatcaaccc cagttaaata tcgctaacgc
     6301 gaatttttta aagaaaaaat tatttaacgg ggtaaacttt ctgggtaact atcaaaagct
     6361 tcatattaaa gtaaatatag caaaataagg gttaaataaa aagactccat atattacaac
     6421 ggggtctttt tatattttgt cactttttaa tgcttaatac cccatctcgt cgtggctgtg
     6481 gcccattccg ccgccttgtg actcttctct ctttggctcc tcggcgattg caacttctgt
     6541 ggtcagaaga atcgcggcgg cactcgctgc gtgctcaaga gcagtccttg taaccttgac
     6601 cggatcgata ataccggcgg atatcatatc ttttactatc ttgtctttat tagcatcata
     6661 accttcgtta tctttactgc tctttatttt gtccacgatc acaagcccgt catccttccc
     6721 ggaatttata gcaatctgtc tgagcggcga ggataaagat gaaagtaaaa ctgaaacacc
     6781 cgcctgaaac tcgttctgga tatcttcaga tgggctctta acccccttgt ctgcaaccct
     6841 agccattgct ctaataagcg cggttccacc tcccgcaacc acaccttctt caatagcggc
     6901 ctttgtcgcc tccacagcgt cctctatctt tagcttcttg tatttcatat cggcttcagt
     6961 ggcggcgcca acctttatga ccgcaactcc accggaaagc ttaccaagtc tctcttgtaa
     7021 tttttcttta tcgaaagacg actcggtggc ggcaatctga gtctttatct gcgcaacacg
     7081 agcctcgatg tttgccttct tccccttacc gcccacgatt gttgtcatat ccttagtaga
     7141 aacaaccttt cttgccctgc cgagcatatc catagtcgca ttctcaagct tcaatccgac
     7201 gtcttcagaa ataacttttc ctcctgtcat gacagcaata tcctcgagca tttcttttct
     7261 tctctcaccg aatccggggg ccttgaccgc gagcgcgctg aacgatcccc taattttatt
     7321 tacaaccaag gtagacaaag catcaccatc aacatcttcc gctattatca ccaattcttt
     7381 tctacccata ccggccattt tttccaaaag cggtaaaatt tcttttattg aagatatttt
     7441 tttatcggtg agaaggacca tagcatcctc atattcggcc tccatcctct cggtgttcgt
     7501 gaccatatac atagacacat atcccttttc aaactgcatt ccctctacca cctcggactc
     7561 gacaccgaat ccctgagatt cttcgacagt tacaactccg tcctttccga ccttatctat
     7621 ggcatcggtg attatctctc caaactcgga tgattccgcg gatattgtgg caacctgaac
     7681 gatctcgttt ctgttcttta tcggtttcgc catttttaca agctcttcaa caacggcttt
     7741 tgtggcgtat tcaattcccg tgcgcacacc catggcgttg acacctagtg caattctttt
     7801 aaatccctca tctatgatcg cttgggtcaa cacaacggct gtagtagtcc catctcctgc
     7861 catgtcgttc gccttacctg cgacatcttt cacaatctcc gctcccatat tctcgatctt
     7921 atccccaaga acaatctcct ttgcgataga cacaccgtcg ttagtaatta tcggcgcgcc
     7981 atatcccttg tctatcacgg cgttcttgcc cttaggaccg atggtaatct ttacggcatt
     8041 agccaccttg tctacgccat gccttagggc ctgcctcgct tcattgttga aaattatttg
     8101 tttggccata tgttatgaat taaaaattat gaataaagaa tcaagagaaa aatatctgga
     8161 ttgccgcggc tcggtcaaga cacctcgcct cgcaatgacg ggaaaccggg attttattct
     8221 actatcgcta atatattact ttcacttaaa ataaaatatt ccttaccgtc cactttaatt
     8281 tcatcggggc catatttggc aaaaataatc ttgtctccga ctttgaccga catgggaata
     8341 cgcttcccgt cgtcgttcat cttgcccggg ccgactgcca cgactgtgcc tctttctgcg
     8401 cgctccttat ctacagtttc tggtatgaaa ataccgctct ttgtctcttt gcccctctcg
     8461 ttttctccta tcgcctccac gacgacacga tcatgaagcg gctgtatagc gattttttta
     8521 ctcatagata tatatttaga gctaataatg ttgataatta tggccccatt ataggctttt
     8581 agccttctta gtcaatgatg aggcctaata acgtcggttg ctttttgctt gtatttgtag
     8641 ctcttttgtg ctactattaa caacaatatg gaaacaataa taacatttct tccttggata
     8701 caaattgccc tttcagcatt gctaatcggt gtcattttga tacaacagag cgaagccgga
     8761 ataagcggta atatgggagg cggtcagcac agaacaaaaa ggggtcttga aaaaggcatc
     8821 ttcattggaa caattgtaat agctattcta ttcgccgctt ccgctcttct tgctatcgca
     8881 tactcgagct tctaacaaaa ctagtcgcgc caaacacaat cggcgagatt attctgttta
     8941 taatttttaa tgaaagaaaa gatatctatt cttctcaaga aagaatttaa tatccctatt
     9001 ccgggttttt tgcgacacga aaaacttaaa ttcatatttc aggacctacc taaaaaagac
     9061 gagcttttgg tcgcaactaa aactttttct aaaaaagaaa gattattctt ctggaccttc
     9121 gcatttttat tttgtagcac gggggcgttt ttgctttggg atataaataa agacttaatg
     9181 atcgagaggc ctgtaaaagg cggggaaatc gtagaaggag tcgcggacat gccgagattc
     9241 ataaatcccc ttctcgccac aggaatggct gatgccgata tcacatctct cgtatattcc
     9301 gggctactta ggccggggcc taacggagaa tatataaacg acctcgcaga gagctatacc
     9361 gtttcgaaag acggtctcgt atataatttc aagataagag aaaacgccac atggcacgac
     9421 ggcaagccgg taacggcgga cgacgtgcta ttcacaatcc aaaaagcgaa agatgtggta
     9481 ttaagaagcc caaagagagc aagctgggag ggcgtcacgg ctctggtcat atccgaaaaa
     9541 gagatccaga tgacgttgaa acagccgttc cagtctttcc tcgagaacgc taccatgggt
     9601 attttgccaa aacacaaatg gaacaatatc gactcagaca aatttgccct cagttatctc
     9661 aatatgagag ctatcggctc cgggccatat aaagttaaaa cgatatccaa gagaacgacc
     9721 gatgatatgc ctgaatacat agaactcatt cctttcagag aatttgcgct aggagaacca
     9781 aaaataagca aaatcacgat tcacttctat caaaatcaag aggagctata tacggcctac
     9841 aaaaaaggca ctgttaccag catgagctcc ccttctccta agtttgttga agaattgaaa
     9901 aatagcaaaa caaacataat acaatcgagc cttcctagaa tatatgccat attcttcaac
     9961 cccaacgaat caaaactttt ggccgacaaa gaggtaaggc gcgcccttga tatggccata
    10021 gataaaaagg gcataatcga caacgtcctt cttggatatg ggacaatcat aaaaggaccg
    10081 atacctccgg actctttagg gtttacagag actaataacg ccgaagccag cacaaccccg
    10141 gcgacaaaaa tagctgaggc gcaaaagata ttagccaaac tcggctggaa gaaaaatgaa
    10201 aagacaggaa tccttgagaa aaaaatctca aaaaaggaaa ctcaagtatt cgaagtctct
    10261 atatcaacga ccgatttcac cgagcttaag acagccgcag gaatggtaaa ggctgactgg
    10321 gagaaattgg gtgctaaagt aaatttacag atattcgaaa aaggagactt ggatcgaaac
    10381 gtgatagagc cgagaaaata cgactccctc ttctttggag aaattgtagg gcgtgatttg
    10441 gatttattct ctttttggca ctcttctcag aaaaaaaatc cgggggttaa tgtttcaatg
    10501 tattcgaata ccaaagttga taagatatta gaatcgttga aagcggagcg tgataatgca
    10561 aaaaaaatta agctattaaa aaatttccaa acagagatag ataaagatat accggcatca
    10621 tttatatatt cacctaattt tatatatcta cttccaaaag aagttaaaaa tgttaacatt
    10681 ttgcctccgg taatcgcatc ggataggttc ttaaatgtct acgagtggta cataaataca
    10741 gaaaaaatct ggaaaatatt ccaataaaaa atatttttaa aaaattataa aagcaggaca
    10801 acgtcaaaag tcctagcaaa gtctcatcgc aaaaagcgat aatcatccca aaaaagggat
    10861 aaataattct aattaataaa tgataaattt tcacggaagg aatggaggcg cttcttcgtc
    10921 cccaaataga gcaagaaggc cggccccacg gagatcaccg gagcgtggat tcgtgagcgg
    10981 aacgaagcgt tttggccaaa aaccaaatga aatatcggcc tcaggaccga taaaaaagtc
    11041 gttccgccag acgagtcaag gccacagaag ctaccaaaag aaaaacatgg tcgaaccgaa
    11101 agccacacct aaggcctatg atgacacaat cagaataatc ccgttgggag gggtagaaga
    11161 aatagggaaa aatatgacgg tcgtcgaata taagggcgac attgtcatcg tggacatggg
    11221 ttttcaattt ccgggtgaag aagccccggg agtagattat ataattcccg atactacata
    11281 cctcaacaat aaaaagaaaa atattaaggg tgtatttatt acgcacggac acttagacca
    11341 cataggcgga atcccttata tcatggacag aatcggcaat ccgacgatat atacccgatt
    11401 gctcacctcc ctgctcatca aaaaaaggca agatgagttt cctcatttcg aaccattaaa
    11461 cttgcgaatt gtagagacgg caaaaacaat gcggataagc gacaacatga cgataaggtt
    11521 ctttaacgtt acgcacacta tccccgactc aatgggcgtt atcatagaaa ctcctcacgg
    11581 gaacattgtc ttcacgggcg acataaaggt tgaccatgag aatggtgagc cgcttccgca
    11641 cgaagttgaa acattcacaa aactcggcaa tgaaaacaac ttggtcctgc tttccgattc
    11701 tacaaacgta gaaaaaccgg gctggtcatt ctcggaaaaa atggtgcacg aaaacctaaa
    11761 gaatataatc agggacgtga agggaagact tataatcgga acattctcat cccttctgga
    11821 gcgtattatg tttatcataa aaacctccga ggagatgggc aaaaaagtcg ttatcagagg
    11881 gaagagtatg aagaacaaca tcgcgattgc gatggaaatg aaacttctaa agataaatcc
    11941 gacaaccata attccaccgg aagaaatcga caactaccca gtggacaaaa tagtggtctt
    12001 ggccaccgga gctcaaggag acgagatggc ggcgctcatg agaatgtctc agaaaaagga
    12061 taaatacatt aagctgaaca aatcagacgt cgtattgctt tcatcttcta ttattcccgg
    12121 caacgaaaaa agcgttcaga aactgaaaga taacctttca agacaaggtg cgactatcat
    12181 tcactacaga atagccgatg tccactcatc cggacatgcg aactatgatg aaactctctg
    12241 ggtccacaaa atgatcaagc caaagtattt cataccgata cacgggcatc attttatgct
    12301 tcgcgttcac ggggaaatag cgaaaattgc aggcacaccg gaggccaata ttgttattcc
    12361 cgacaacgga atgataattg agataggaga caaaggaaac agcataaaag tgtcaaaaga
    12421 gtcggctccc gccggtgtgg taatggttga cggtttgggg aatggagacg taagcgacgt
    12481 cgtaataaga gacaggcaga tacttgccga agacggaatg ttcgtaataa tcgccgtcat
    12541 tgatatgaaa accggaaaga taaggcaatc gccggatata atctcgagag gttttattta
    12601 tctaaaagag tctcaggatc ttcttaagga tgtgagaggt ctcgccaaaa gaacgataga
    12661 aagtttagct gtagatatgc accccataaa tcttgattat gttaaaaata atttgcgaga
    12721 aaaaataggc aagtatctta tgcaaaatac agggaagaga ccgataatat tgccggttct
    12781 gctggaacta taataaagaa aaagccggga cttattcccg gcttttcttt atattatgtt
    12841 aaaattaaag catgacatta aaagaaaaaa ggtggatgct gttttttata tattttacga
    12901 cctttctgac ctctatcggg tatgccttta taatttatat aaattcgtca tttctgcaga
    12961 aattcgtaag tagcgaatat attggagcgc tatacggggt aagttctctt gtaacgctta
    13021 tcattctaac aagaatatca tacgcgctaa aaaagatcgg gcacttcaaa gtaatgctcg
    13081 ggataatcac gcttgaaaca ttgtccgtct tttgttcggc catactcgat atgtattctt
    13141 tttccggtat cacgaccgac tatttgcgat tgtctttatt tgttatactc gcctttttaa
    13201 tatttaacac gacggcgata ttactcagat tttccttaga tatttatctg gaaaaatact
    13261 caaagaacag agaaaccggt aaagctcgcg ggatcttcct cacgacgatg aacctggcga
    13321 tagcagtgtc tccgtatatg gtcgggaaaa ttctaacgaa tggagatttt tggagggtct
    13381 atctggtcgc atcggctata ttcttgccgg cgattattat aacaggcaca aaactacgca
    13441 aggttagaga tgtggaatac cacgacacca aattcacaat agggctcaag aaagtctgga
    13501 aaaacaaaaa tatacgttct atatttatga cgaatttcct gcttgaattt ttctattcgt
    13561 ggatgactat ctatactccg atataccttt tcaagataat ggggttctcc tgggagaaca
    13621 taggaacaat gttcagcctc atgctcactt cttttgttat tcttcagttg cctctcggaa
    13681 aaattgcgga caaatatctc ggagaaaaag agatactcac cgcggggctc atcattacgg
    13741 gcctctctac atcgtatctc gcttttatag aaaagccgga attcatcgta tggacagcgg
    13801 ccctatttat gacaagggtt ggcgccgccg cgatagaaat aatgaacgag acctacttct
    13861 tcaaaaaagt atctcccgag gattcagatg tgataggttt ttttagaaac gcatcccccc
    13921 tcgcatacat aatcggcccg atgacggctt ctgttctcat ttattttata gacttcaaat
    13981 acatcttcgc aattttagga gttattatgc tcgttggcat aagatatagc ctcgcgataa
    14041 aagacactct ctaggcggag ccgagtcgac attttttgtt taagctgttt ctgagcgaac
    14101 aatacttaca tttttcatct tcacacctcc tatcccaaaa atccagattt aatatttcct
    14161 cggatacctt tttaataagt tccctcaagc cggagacatc ttcggaattt atgtctatgc
    14221 gttccttcct gtatgctccc tttgggtcag gcattataaa atctatctcc cctgttaccg
    14281 catgatattt ggcgttatca taattatcta aaagtagtct ataaaataat agctgcctct
    14341 tcatatcccc atcagaattc ttcgtcttgc cctctatctc gttcctgctt tttattttac
    14401 cggtcttata atccacaaca tttacgttat tggccgcatc aagtatctct attttatcta
    14461 tcttgccggt tagcgttacc ggtataccgg gaagaacaat gtgaaccttg aattcattta
    14521 agatctctat attccaatct tttatatatt ttttgtagta accccttaac gcctctttgc
    14581 ctcttttcaa tgtctccttc aagtcttcgt cagacaaagg ttcttctttc aaatattttt
    14641 caaatatctg aagcaatttt tgaacgccga tatcatcgtc ttcttttaat ttatcgaaga
    14701 agtctttcag agcataatgt atcgcgtttc cgaacatcaa aaacttatcg ggagcttccg
    14761 gaattcttat caggttgcgg taaaaataac tccacggaca ttctaaataa ttatttaagg
    14821 cggtcacaga aaatccctgt gacaaaaata tttcatttag gaatttctta tcatcggcgc
    14881 ttgtcccgcg attaaccttc ggctcaaaac tttttacaag cacgtccttg gctaattccg
    14941 gagaagaaac ttcgtctttc aggtctaaaa attgcgggtc aatctcctcg ataaactgtg
    15001 aaggcaacaa caacttgccc tctttatctt tttttgaata actcaaaaag atatctttct
    15061 tggcgcgcgt aagagccacg tagaacaatc gtctttcgtc atcgttgccg ccgtcagtac
    15121 tcgcgccgag cggtagttta aaatgttttt ttgtgcgcct atttccccag tgaccgtcat
    15181 ataagcccaa aatatatacg tgatcgaact ctagcccctt ggacctatgc gccgtcataa
    15241 gatgaaccgc atccatgccg gatctctctc caccagccga cctcaaaagc cggcggccct
    15301 tggcggcctc caagaaatcc ata
//
LOCUS       DAOTEX010000010        14703 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3118
            NODE_2816_length_14703_cov_4.133602, whole genome shotgun sequence.
ACCESSION   DAOTEX010000010 DAOTEX010000000
VERSION     DAOTEX010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868142
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 14703)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14703)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 883
            CDSs (total)                      :: 833
            Genes (coding)                    :: 829
            CDSs (with protein)               :: 829
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14703
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2816_length_14703_cov_4.133602"
                     /isolate="AMR_MDS_3118"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(40..1986)
                     /locus_tag="P5056_01085"
     CDS             complement(40..1986)
                     /locus_tag="P5056_01085"
                     /inference="COORDINATES: protein motif:HMM:NF036997.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62349.1"
                     /translation="MAEIKMATVIPIGDSVFRDSLSYFTMRDIEPGSLVFVPIRKKRI
                     PAILVSTQLIKDVKTELKRSDFELKKIEKASEERFFSKEFMEAAEEAADYFGANLGQI
                     LSSYVPNNLFKGYEKNLHKIKTKKKEPPAEETTAERKHTVELVQAPLEDRIAFYKSLI
                     RENFARKSSVFLCLPTMHEVNIFADNLKRGIEDYALVLRPEDSPKKLISASQTIKERA
                     HPLLVIGTPVFACFLDNSFETVIVEKESSPAHKTIFRPFIDSRIFLEKLARKLSLRLI
                     FGDTVLRLETIHKKETDEYSPSLLFKYRLISRTMPRLLDVKKDKNNFDTRAISSELLE
                     LLEGAIEAKKNTVLLTTKKGLSGATICGDCKTIVSCKNCGSTTTLHRKENSNVFYCHK
                     CGTGQSASVLCNKCRSWNLRPVGFGTERVEDEVRSLFPNANVIRMDGESVKTHKQGKE
                     MMSNFEENKGSVIVGTEMALFYLTKPVENIAIISTDSLFFIPDFKINERIFNYLVNAK
                     LMAENNFVIQTRCADHPVFEKIVRGDALQFYKEEILLRKNFSYPPFKLFIKITGEGKE
                     DVVAKEFDELAVFLKKWNPDKFRSATNLQGGKARMNLLIKVNPLNWPEKPTGSDDPEK
                     MSLPEILKILPPRFAVKVDAESLL"
     gene            2059..3198
                     /gene="mnmA"
                     /locus_tag="P5056_01090"
     CDS             2059..3198
                     /gene="mnmA"
                     /locus_tag="P5056_01090"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018497733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HRY62350.1"
                     /translation="MTGRHIAQKAKKIEAPKRVFVGMSGGVDSSVSAALLKEAGYEVI
                     GVFIKVWSPDWLPCDWREERRDAMRCAAILEIPLLTLNLEKEYKEEVVDYMIREYKAG
                     RVPNPDVMCNRSVKFGGFFNEAMKMGADYVATGHYAQTRIKNYELRITNEKIKKYELL
                     AGEDRNKDQSYFLWTLKQEQLSKTLFPVGGLKKEEVRKLAEKFGLPTATKKDSQGLCF
                     LGKIDVKDFLKRYINAKEGKVLNEKGKVVGHHDGAVLYTIGERHGFAIDNKKPDDAPY
                     YIVGKDIKKNTITVSHNKASGNSAEREIEISDLSFTLGEAPVKNKKYSARIRYREDLK
                     KCEIKKISPKKATIVFDEPEESVSAGQSLVLYDGDICLGGGVIIG"
     gene            3342..3506
                     /locus_tag="P5056_01095"
     CDS             3342..3506
                     /locus_tag="P5056_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62351.1"
                     /translation="MHNLELAGAMEFAEAEANAVVNAELGGSDDIEAKKWRHLDAYTN
                     ALQGYLGSIE"
     gene            3635..3709
                     /locus_tag="P5056_01100"
     tRNA            3635..3709
                     /locus_tag="P5056_01100"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3667..3669,aa:Val,seq:cac)
     gene            3835..3907
                     /locus_tag="P5056_01105"
     tRNA            3835..3907
                     /locus_tag="P5056_01105"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3868..3870,aa:Ala,seq:ggc)
     gene            4180..4590
                     /locus_tag="P5056_01110"
     CDS             4180..4590
                     /locus_tag="P5056_01110"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HRY62352.1"
                     /translation="MQLELKDFDKDFFETLQDKEEIFMSDKGVYYTIIYDGHKAGVVG
                     YIPVGTSGDTGFTQTVLAPEFRGKGLSGAAKELMVKKHGLKTLLATIRKGNIVSIKAN
                     EKIGFKMLSEEKMAELRAKGFLKEGDIRMEKRYK"
     gene            complement(4625..5164)
                     /locus_tag="P5056_01115"
     CDS             complement(4625..5164)
                     /locus_tag="P5056_01115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62353.1"
                     /translation="MNENLKRYEQFKERQRQAYRDYVRKNEERKEREVAEMAEKYAEY
                     DKKLELVKELENKIANAENEEGLLYIEHVRRQRELRALLTPKLVLYRTLKSIDEHNSK
                     YKPEHAGYWNFYERHNGEIVLHQCGWSMAGNPEMNYFNETGEMMFCITTGKHGLTRIS
                     FEEARDKLMSGEGFYRSQR"
     gene            5358..5672
                     /gene="rpsP"
                     /locus_tag="P5056_01120"
     CDS             5358..5672
                     /gene="rpsP"
                     /locus_tag="P5056_01120"
                     /inference="COORDINATES: protein motif:HMM:TIGR00002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HRY62354.1"
                     /translation="MIRLQRVGKKHDPSFRVLLTDSKNSTKSGKFIEVLGHYDARGGK
                     TELKKDRIKHWLSVGAKTSVTLHNILVNNKVVEGKKKSAMPKRKKSAEAKTAAPAPTP
                     AA"
     gene            5918..6247
                     /locus_tag="P5056_01125"
     CDS             5918..6247
                     /locus_tag="P5056_01125"
                     /inference="COORDINATES: protein motif:HMM:NF024483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HRY62355.1"
                     /translation="MEKDQEFLELVAKALVDNPQDIKVKRVVDEMGVLLTLDVNPADM
                     GKIIGRSGNTAKAMRTLLRVVGMKHNARVNLKINEPEGSTRMMSAPMSPQSASKTVDE
                     AMMDLKL"
     gene            6306..7619
                     /locus_tag="P5056_01130"
     CDS             6306..7619
                     /locus_tag="P5056_01130"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRY62356.1"
                     /translation="MEFVLECRQVGTFTKNTEGVVLMAKTLMTVRRDGLRIITKRLSY
                     TQQVMCSVTSLMGSASDPAELLGIAHYFEHMVFQGTKTRTREEIERDIRRYCISHNAD
                     TSFLRTRYYGECAKINFEKTLEVLLDIYCNPIFPEDRVENERGVIGTEISMRKNEEDI
                     LAFENFLVSLYGAKSLPGRGIIGTKETISTVNRNLLLDMHKTYYSPENSVIVVVGDVS
                     HRDVVKNVNQFFPIGKPSAYRQRFDWPNDPDSPPAEPLVVDEKQGREYSRVYFACKIP
                     SGSLRDRLVMNLLAKCLDRVIYDELRQKRGLVYTAGASDYYKRPLISTFYSCAQTEVH
                     NFAQVRDILVDYVLNEKICESDFEIFRESKFVTSSIALETSREWNDAILSQIISGNSD
                     LGFIDNYFEKISKVIEGITFDDLLKMRLELIREDRMSFSIVTNKD"
     gene            7694..7912
                     /gene="rpmF"
                     /locus_tag="P5056_01135"
     CDS             7694..7912
                     /gene="rpmF"
                     /locus_tag="P5056_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005890386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HRY62357.1"
                     /translation="MVVRMRHTRAHTANRRSHHALKGAGLTKCVKCAAMARMHMVCPS
                     CGFYKGSEKVNTMAKIQKKNKKEAKKSK"
     gene            7992..8888
                     /gene="nusB"
                     /locus_tag="P5056_01140"
     CDS             7992..8888
                     /gene="nusB"
                     /locus_tag="P5056_01140"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HRY62358.1"
                     /translation="MANRHLARSIVLQTLFEWDFGGRNNSELRNILHKNVEEFGPGLT
                     DFSFIESLLESVVNKMDAMDSIITKAAPEWPIDRISIIDRNVLRIGLYELIFMDKSEV
                     PAKVAINEAIELAKTFGGESSGKFINGVLGTIYKEMGEPGKDEISRPKKNSAGVDVTK
                     LPVDRLGGAVVYAIDEGKIFLAMVHDVFGYWTLSKGHIEEGESEEEGTMREIKEEIGL
                     DIEIGKKVGENEYVANDPERGKIRKKVSYFLGKTDKKELELKDGGGLDEAKWFEASEV
                     ADLKIYNDIVPIITEAIKEIIL"
     gene            8970..9704
                     /gene="rnc"
                     /locus_tag="P5056_01145"
     CDS             8970..9704
                     /gene="rnc"
                     /locus_tag="P5056_01145"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HRY62359.1"
                     /translation="MVIKKFRRASNHMNAPDFSKFEKKISVEFKNKGLLKQAFTHRSY
                     INENKGTGLTHNERLEFLGDAVLELVITEFLFREFPDKQEGELTSLRAALVNTVTISK
                     IAGDVGMNDFLLLSKGESKDVGRARQVILANTFEAFIGALYLDQGYESAKKFISDNLF
                     SELKTIVEKSLWKDSKSKLQEKSQEVLSITPAYKTLNEVGPDHNKIFTVGLFLGEEQV
                     SEGKGHSKQEAQEEAAKSALKKKGWE"
     gene            9946..10983
                     /locus_tag="P5056_01150"
     CDS             9946..10983
                     /locus_tag="P5056_01150"
                     /inference="COORDINATES: protein motif:HMM:NF016924.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HRY62360.1"
                     /translation="MSKQHKLTFNPKKVTKDLLGVLPVRARDIIKKRYGLESSSEGMT
                     LEAIGETYGITRERVRQIENFALNSIRKSDAYTANAVSFEELKKHMDEYGGVVHEQDF
                     LDHLTSDKELQNHVSFYLELGSDFIKLKEDEEFAHRWTIDEELSGKVHNSLRKLYESL
                     SEDDLISESEMIVKFLDHLEETVKDIRNEELAKRWIKMSKKIGKNALGEWGIAHSPNI
                     RVRGIRDLAYLVLRKHGSPMHFREVAKAIKQIFGKDVNEATCHNELIKDKRFVLVGRG
                     LYALAKWGYSQGTVRDIIKTIIKDKGPQSRDEILKKVLRERYIKENTVLVNLQNKKYF
                     KKDKNGKYLIV"
     gene            10980..12194
                     /locus_tag="P5056_01155"
     CDS             10980..12194
                     /locus_tag="P5056_01155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62361.1"
                     /translation="MIQEILVQLLQTFGEVLFRAFAFALPFLAFIIAWSLWRYYIVRN
                     FIAKAEWVMLEVKLPKEHQKTPQAMEVVLGAFHQFPTPPTWYDYWWLGKVPLWFSLEL
                     VSINGAVHFFMKIQKSQRSIIENHIYSQYPQAEIHQVPDYVGGINFEAPDSKWKMFGM
                     EHVLVREDAYPIKTYVDYGLDRELLKEEQKVDPITSTIEFLGSLGEDEQAWIQIIIMA
                     AQPRFPKKDTWFGKEDWKGNAKELLKKLLKRDAAPGFGPMMMLSPGERATVEAIERSM
                     SKLGFDCGIRTLYFGKDKKFNPQNIANLFATFRQYGSVTLNSFKPVRITSFNWWVDPF
                     GWKLKELKSSLFRAYVSRGYFYRPFKNVPPFTLNSEELATIYHFPGAVSVTPTFSRIE
                     SKRAEAPINLPI"
     gene            12354..13403
                     /gene="mltG"
                     /locus_tag="P5056_01160"
     CDS             12354..13403
                     /gene="mltG"
                     /locus_tag="P5056_01160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HRY62362.1"
                     /translation="MHPDLSNIMMLESEEPNEPGGEKAPSPKNIFNLNEKARKVLLAV
                     SIVLIVILAINYNFFAAPAEHPGGTKFAITSGMTISEAAEYLSENNIIKSPTVFKTLV
                     FIMGGRSGVIAGSYFIEHPVSVFRIAMMVTTNGYGIKMYKVTIPEGLSNRQIADLLAK
                     NLDNFDKAKFLKLAKDKEGYLFPDSYFFEPESTPEKVIEMMNDNYNEKIKMVNLDIVF
                     FNKPIKDIITMASILEEEARTENTRRMIADILWRRIERGMSLQVDASFVYLLGKGSAE
                     LTVDDMKIDSPYNTYKYKGLPPGPITNPGLASILAATKPLKNKYWFYLSDKDGIMHYA
                     VTFEEHKLNKERYLR"
     gene            13727..>14703
                     /gene="gap"
                     /locus_tag="P5056_01165"
     CDS             13727..>14703
                     /gene="gap"
                     /locus_tag="P5056_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HRY62363.1"
                     /translation="MRIAINGFGRIGRQFLKIALENQELEVVAINDLGDLDNLAYLLE
                     FDSVYRNYDKKVETKNGNLVVGGKTIKFLQDPEPAKLPWRDLVIDVVVECTGRFTSSD
                     AAKVHLDAGAKRVVISAPAKDDMTPTCTPNVNVSALTESKITSNASCTSNSIIPIAAI
                     LGENPGIKLSLINTVHGYTAEQSLVDGPMPKLHKDMRRGRAAAQNIVPTTSGAASAVG
                     KTIRSMEGKFDGMALRVPVPAGSILDFTFISEKKTSAEEINDILKEAAAKPEWQGIIT
                     VTDKPIVSSDILGNTHGSIVDLNLTRVVGGELVKVMAWYDNEWGYASMLV"
BASE COUNT         4279 a         2911 c         3546 g         3967 t
ORIGIN      
        1 agcttgtcga ggaatgacaa tccgggtcga tttaattttt tagagcaggc tttcggcatc
       61 caccttaacg gcgaatcggg gaggcagtat tttgagtatt tcgggtaggg acatcttttc
      121 cgggtcgtca ctaccggtcg gtttttccgg ccaatttaac gggttcactt ttataagcag
      181 gttcatacgc gctttgccgc cctgtagatt tgtcgccgat ctgaatttgt ccgggttcca
      241 tttttttagg aagaccgcca gctcgtcaaa ttcttttgcg accacatctt ccttgccttc
      301 gcccgtaatt ttaatgaaaa gtttgaatgg tggataactg aaattttttc gcagcaatat
      361 ttcttctttg tagaattgta gggcatcgcc tctgactatt ttttcaaata caggatgatc
      421 cgcgcatctt gtttgtataa caaaattatt ttccgccata agcttggcgt ttaccagata
      481 gttgaatatt ctttcgttta ttttgaagtc cgggatgaaa aagaggctgt ctgtcgatat
      541 gatcgcaata ttttccaccg gttttgtaag ataaaagaga gccatttccg tgccgacaat
      601 tacagaccct ttgttctctt caaagttaga catcatttct ttaccctgct tgtgagtttt
      661 taccgattcg ccgtccattc taatcacgtt tgcattcggg aaaaggcttc tgacttcatc
      721 ttctacgcgt tctgtgccaa atcccaccgg gcgcagattc cagcttcggc acttattgca
      781 aagcacgctc gcgctctggc cggtcccgca tttatggcaa taaaagacgt ttgaattttc
      841 ttttctatgt agcgtggtcg tgcttccgca atttttgcat gaaactatcg ttttgcagtc
      901 accgcatatt gtcgcgccgg aaagcccctt ttttgttgtc agaagaactg tattcttttt
      961 tgcctctatc gcgccttcaa gcagctcaag tagctcactt gagatggccc tagtatcaaa
     1021 attatttttg tcttttttaa cgtcgagcaa tctcggcatt gtcctgctta tgagcctgta
     1081 tttgaaaagc agagacgggg agtattcgtc cgtttctttt ttgtgaattg tctctagccg
     1141 taaaacggtg tctccgaaga tgagccttaa agacaatttt cgggcgagtt tttccaggaa
     1201 aatccttgag tctatgaatg gcctaaaaat ggttttgtgt gcgggggaac tttctttttc
     1261 tacgatgacc gtctcaaagc tattgtctag gaaacaggcg aatacagggg ttcctatcac
     1321 gaggagcggg tgcgccctct cttttatggt ctggctggcg cttattagtt tcttgggaga
     1381 atcttccggt cgcaggacaa gcgcatagtc ttctatgcct cgtttgagat tatcggcaaa
     1441 aatatttacc tcgtgcattg tgggcaagca caaaaagacg gaagactttc tggcaaaatt
     1501 ctctctgatg aggcttttgt agaatgctat tctgtcctca agcggcgctt gcacaagctc
     1561 cacggtatgt tttctttccg cggtcgtttc ttcggcaggg ggctcctttt tctttgtttt
     1621 tattttgtgt aaattctttt cgtaaccctt aaataaattg ttcggcacgt aggagcttaa
     1681 gatttggccg aggttcgccc caaaataatc cgccgcttct tcagccgctt ccataaattc
     1741 ttttgagaaa aatctctctt cactagcctt ctctattttt ttaagctcga agtcagatct
     1801 ctttagctcg gtcttcacgt cttttatgag ttgggtagaa acgagtatcg cagggatcct
     1861 cttttttctt atcggcacga acaccagaga gccgggttca atatctctca ttgtgaaata
     1921 tgaaagtgaa tctctgaaga cagagtcgcc tatgggtatg actgtcgcca tttttatctc
     1981 ggccatgccc ctattttgtc aaaaaggagg gccggggaca ctattgaccc aatcgaatat
     2041 aattgttagt ttcttctgat gacagggaga cacatcgcac aaaaagcgaa gaaaatagag
     2101 gccccaaaaa gggtctttgt cggcatgtcc ggaggagtag atagttctgt gtcggcggca
     2161 ctcctaaaag aggccggtta tgaggtaata ggagtattca taaaagtctg gtctcccgac
     2221 tggttgccct gcgactggag agaagagagg cgtgatgcta tgaggtgcgc cgcaatactt
     2281 gagataccgc ttcttacact taatttagaa aaagaataca aagaggaggt tgtggactat
     2341 atgatacgcg aatacaaggc cggccgagtc ccaaaccctg atgttatgtg taatcgctcg
     2401 gtaaagttcg ggggattttt caatgaggcg atgaagatgg gggcggacta cgtcgctacg
     2461 ggacactacg cgcaaacaag aattaagaat tacgaattaa gaattacgaa tgaaaaaata
     2521 aaaaaatacg agcttttggc cggggaagat agaaataaag atcagtcgta ttttctttgg
     2581 acgctcaaac aagaacaact ttcaaaaact ttgtttcctg tggggggtct caaaaaagaa
     2641 gaggtgagga agcttgcgga gaaatttggt ctgcctacag caacaaagaa agatagtcag
     2701 gggctttgct tcttgggcaa aattgacgta aaagattttt taaaaagata cattaacgcg
     2761 aaagagggga aggtcctaaa tgaaaaaggt aaggtcgtgg gtcatcatga cggagccgta
     2821 ttgtatacga tcggagagcg ccacggtttc gcaatagaca acaaaaaacc ggatgatgcg
     2881 ccttactata ttgtaggcaa ggatataaag aaaaatacga taaccgtttc tcacaacaag
     2941 gcttccggca attcggcaga gagggagatt gaaataagcg atttgagttt tactctcggc
     3001 gaggctcccg taaaaaataa aaaatattcg gctcgaataa gatatcgcga ggatctgaaa
     3061 aagtgcgaaa ttaaaaaaat atcgccgaag aaggcgacga tagtttttga tgaacccgag
     3121 gagtctgtgt ccgccgggca atctctcgtg ctttatgacg gcgatatttg tcttggtggt
     3181 ggtgtgataa tcggctaaag aaattttgta aataataatg gtagcaactg cttgtcgccc
     3241 tttttgttgc gttttttcgc cccttggggc cacgaatgac attaattata agagtggtat
     3301 aattagggtg tgtcgatttt tattaattaa tcgttaataa aatgcacaac ctagaactcg
     3361 caggggcaat ggagtttgcg gaagcggagg caaacgcggt cgtcaacgca gagttgggag
     3421 gttcagacga catagaggcg aaaaaatgga gacatttgga cgcctacaca aacgcccttc
     3481 agggatattt gggcagtatt gaataaaagt ccctgacggt tatatttatc tttgaagcca
     3541 agtttaggct tggtgcgggt tctattagac aatattaaag agtaattagc agagctggct
     3601 agagcgttcg cttcattcac ccgccataat ttttgcacgt gtagctcagt ggtagagcgc
     3661 tccgttcaca tcggagaggc cgtaggtccg atccctacca tgtgtaccag gaaaaaatta
     3721 aggagggcag gcgcggtggt atatgcgata gcaaaattat ttgtggccaa gtcctgtatt
     3781 actcatagtt gattttcaaa acaagattcg ttagtataga aaaacaataa atttgggccg
     3841 ttagctcact tggtagagcg catccatggc atggatgagg tagcaggttc gatccctgta
     3901 cggtccactg gtaaaaacat taacgccgaa aggcgttttt tgtttttttc agtggaccgt
     3961 aagcaaagca tcttaaatgc tttgcgtcag gggtcgaagc cccgatgagc attttttaga
     4021 gtgcctcgca cgaataaaaa atccatcggt gggtactgac cctgtaaggg tcgatccctg
     4081 tacggtccac aaaataaaca tcccgggtgc gcaagcactc aggtgttttg tttatctctc
     4141 gttttttgtt tataatttca acacaaccta aaaaaataaa tgcagctaga actaaaagat
     4201 tttgataagg atttttttga aacgcttcaa gacaaggaag agatttttat gtccgacaag
     4261 ggtgtctatt acacaattat ttacgatggt cataaggccg gggtcgtagg atatattccg
     4321 gtggggacat cgggtgacac tggttttacg cagactgtct tggcaccgga gtttcggggt
     4381 aagggtttgt ccggcgccgc taaggagctt atggtcaaaa aacacggcct aaaaacattg
     4441 cttgctacca taaggaaggg caatatcgtg tcaattaagg ctaatgagaa gatcggattt
     4501 aaaatgttga gcgaagaaaa aatggctgag cttcgagcga aaggctttct gaaagaaggg
     4561 gatataagga tggagaagcg ctataaataa aaaaataacc caggcgatgt gcctgggttt
     4621 tgctttatct ttgggagcgg tagaagcctt ctcccgacat gagtttgtcg cgagcctctt
     4681 cgaacgagat gcgcgtgagc ccgtgcttgc cggtcgtgat acaaaacatc atctctcctg
     4741 tttcgttgaa ataattcatt tcggggttgc ctgccattga ccacccacat tggtgcaaaa
     4801 cgatctcgcc attgtgacgt tcgtagaaat tccaataacc cgcgtgctcg ggcttgtatt
     4861 tggaattatg ctcatcaata ctcttcagtg tccgataaag gacgagcttg ggagtaagaa
     4921 gtgcacgaag ctctcgttgc cgacgcacat gttcaatata aagcagacct tcctcatttt
     4981 cggcattggc gatcttgttt tccagttcct ttacgagctc cagctttttg tcatactcgg
     5041 catacttttc ggccatttcc gcgacctccc tttcttttct ctcctcattt ttccttacat
     5101 aatcacgata tgcttggcgt tgtctctctt taaattgttc ataccgcttg aggttctcgt
     5161 tcacgatatt tgccctcctt ttaaatgttc aattgttcgg tcttatccta tatcattatt
     5221 tccttgtgtc aaatagaaaa aggagggggt tgaccccgta gtgatcctgt agtagaggtg
     5281 cgtggcgcgc cttcactacg ggactactac agggttgaca ataaacaggt ttttttgcta
     5341 gtataccccc tatgttaatg atcagattac aacgagttgg caagaagcac gatccatctt
     5401 ttcgtgtact tttgaccgat tctaaaaaca gcacaaaaag cggtaaattt atagaggttc
     5461 tcggacacta tgatgccaga ggaggaaaga cggaattaaa gaaggataga ataaagcact
     5521 ggctttctgt aggcgcaaag acttccgtaa ctcttcacaa catattggta aataacaaag
     5581 ttgtagaagg aaagaaaaag agcgcaatgc caaagagaaa gaagagcgca gaggccaaaa
     5641 cagccgctcc agccccgaca ccggccgcat aaattttaat attaaaaatc cccacaatgc
     5701 tgtggggatt ttttgtcggt ggagcttgcc tgccccgtag gaagcatgct tagggggaga
     5761 tataaatgaa tatcgcgtga gatttaacgg ggggtcattt aagaccttat acacagcttt
     5821 ccttcggggt tttcttttta gtttgtttat gctataaata tacccaagca gtgcagggtc
     5881 gaaatgactg cttttacaaa aagattagaa ttgcaaaatg gaaaaagatc aggaatttct
     5941 agaattagtt gcaaaggcat tagttgacaa tcctcaggac atcaaagtca agagagtagt
     6001 cgatgagatg ggagtcctcc ttacgctcga tgtaaatcct gcggatatgg gcaagatcat
     6061 cggtagaagc ggaaacaccg ccaaagccat gcgaactctt ctccgagttg taggaatgaa
     6121 gcacaacgct agagttaatc tcaagattaa cgaaccggag ggaagcacaa gaatgatgtc
     6181 agctcctatg agcccacagt cagcttctaa aacagttgat gaggccatga tggatctcaa
     6241 actctaagac ataatttaga tttgaataaa aacagctccg agtaatcggg gctgttttgc
     6301 tttttgtgga gtttgtgctt gaatgcaggc aggttggtac atttacaaaa aatactgaag
     6361 gggtggtcct aatggcaaag accttgatga ctgttcgacg tgacggcctt cgtatcatta
     6421 cgaaaagatt gagctatacc caacaagtta tgtgttcggt aacctctctt atggggtcgg
     6481 ctagcgaccc tgcggaatta ctcggcatcg cccactattt tgaacacatg gtgtttcaag
     6541 gaacgaagac tcgcacgcgc gaggaaattg aaagggatat taggagatat tgcatctctc
     6601 acaatgccga taccagtttc cttcgtacca gatattatgg cgagtgtgcc aagattaatt
     6661 ttgaaaagac actcgaggtt ctgctcgata tttattgtaa ccccattttc ccggaggatc
     6721 gtgtcgagaa cgagcgcggt gttatcggga cagagatctc gatgagaaag aatgaagaag
     6781 atattctggc gtttgaaaat tttcttgtct ctttatatgg agccaagagt ttgcccggac
     6841 gcggtataat cggaacgaag gaaacaattt caaccgtgaa caggaacctt cttcttgata
     6901 tgcacaagac ttattattct ccggagaatt ccgttattgt cgtcgttggg gatgtgtccc
     6961 accgcgacgt cgtgaagaac gtcaatcaat ttttcccgat tggtaaaccg tccgcataca
     7021 gacagcgttt cgattggccg aatgacccgg attctccacc ggccgaacct ctggtcgtcg
     7081 acgagaagca gggaagagag tattctcgtg tatatttcgc ctgcaagatt ccgagcggaa
     7141 gtctgcgaga ccggctcgtg atgaatcttc ttgccaagtg cttagacagg gtcatttatg
     7201 atgagcttcg ccaaaaaaga ggcctcgtct acaccgccgg agcgagcgat tattacaaaa
     7261 ggcccctcat ctctactttc tattcgtgtg cacagaccga agttcataac ttcgcgcagg
     7321 tcagagatat tctcgttgac tatgtgctca atgaaaaaat ttgtgagagc gattttgaga
     7381 tctttcggga gtcaaagttt gtcacaagct ccatcgcgct ggagacttcc agagaatgga
     7441 acgatgccat cctttctcag attatttccg gcaattccga cttgggattt atcgataatt
     7501 attttgaaaa gatatctaag gtgattgagg gaattacttt tgacgatctg ctgaaaatgc
     7561 gccttgaact cattcgcgaa gatcgtatgt ccttttcaat cgttaccaac aaggactagt
     7621 cctatttagc cgccctaact acgggcggct tttatcttgc aaaatctata aaatagtgta
     7681 atatcttccc catatggtag taagaatgag acatacgcgg gcacataccg cgaacaggag
     7741 atcccatcac gctctcaagg gagcgggcct tacaaaatgc gtaaagtgtg cggccatggc
     7801 tagaatgcac atggtttgcc catcttgcgg attctataag ggttcagaga aagtgaacac
     7861 gatggcgaag atccagaaga aaaataagaa ggaggccaaa aaatctaaat aaataattat
     7921 ttagattttt tagcttgccg gatgttttat taggatagcc ggcatccgtt taatgtaatt
     7981 ttgaaaatat tatggccaat aggcatttag cgagaagtat cgttttacag actctcttcg
     8041 agtgggactt cggcggccgt aacaattcag aattaagaaa tatccttcat aaaaatgtag
     8101 aggagttcgg tcccggcttg accgactttt cttttatcga atcgcttttg gaaagtgttg
     8161 taaacaagat ggacgcaatg gattccatta tcacaaaggc ggctccggag tggccgatag
     8221 acagaatatc tataatagac agaaatgttt tgagaatagg tttatatgaa cttattttta
     8281 tggacaagag cgaggttcct gcgaaggttg ctataaatga ggccatagag cttgcaaaga
     8341 cttttggcgg cgaaagcagt ggaaaattta taaacggtgt tcttggcacg atttacaaag
     8401 agatgggaga gccgggcaag gatgagatat ctagaccaaa gaaaaacagc gccggagtag
     8461 atgtaacaaa actccccgtg gacagacttg gaggcgcggt ggtatacgcg atagacgagg
     8521 gaaaaatatt cctcgccatg gtgcatgacg tattcggata ttggactctt tcaaaaggac
     8581 acatagagga gggtgaatca gaggaggagg gcactatgag agagataaag gaagagattg
     8641 ggctcgacat agagattggc aagaaagttg gagagaatga gtatgttgcc aacgatcccg
     8701 aaagaggaaa gataaggaaa aaagtatcct atttcctcgg caagacagac aaaaaggaac
     8761 ttgaattgaa agatggagga ggcttggacg aagcaaaatg gtttgaggcg tcggaggtcg
     8821 ctgatttgaa gatttacaat gacatcgttc cgataattac agaggcgata aaagaaatta
     8881 tattgtaacc cgccaaaatt ttttaccaat catttatatt atagacaaat aaaagacagc
     8941 ctatctaaaa ttttttaata attattaaaa tggttattaa aaaatttagg agggcaagta
     9001 atcatatgaa cgctcccgat ttttctaaat ttgaaaaaaa gatctctgtt gaattcaaaa
     9061 acaagggctt gctgaagcag gctttcaccc accgatctta cataaatgaa aacaaaggaa
     9121 ccggccttac tcacaacgag agactggagt ttttgggtga cgcggttttg gaattagtca
     9181 ttacggaatt ccttttcaga gaatttccgg acaaacagga aggggagctt acgagcctcc
     9241 gcgccgctct cgtgaacacg gtcacaattt caaagattgc cggagacgtg gggatgaacg
     9301 acttccttct cctctctaag ggcgagtcaa aggatgtggg aagagcgaga caggttattc
     9361 tcgccaatac ttttgaagcg ttcatcgggg cactttatct ggaccaggga tacgagtcag
     9421 ccaagaaatt tatttccgac aacctatttt ccgagcttaa aacaatagtt gaaaaatcac
     9481 tctggaaaga ttccaagagc aagcttcagg aaaaatctca ggaggtgctc agcatcacac
     9541 cggcttacaa aaccctgaac gaagtcggtc cggatcataa caaaattttt acggtggggt
     9601 tgttcttggg agaggagcag gtatcggaag ggaagggcca ttcgaagcag gaggcccaag
     9661 aggaggcggc caagtccgcg ttgaaaaaga agggttggga gtaattttgt actcttgtcc
     9721 cggtaggaaa gtccgccttg acaatgttgt aatgtaaacc tgagtctgcg aattcgccta
     9781 tggttttttg taatttaaca ttgatttata ggggtaactt cctacgggac tgcgcgactc
     9841 gcttttccga agcgagtcgc ttgactttct gtggccaata agtagaatga cgttattgac
     9901 gaattaaaca aagtattata acagtattat taaacaacaa ggatcatgtc taaacaacac
     9961 aagttaacgt ttaatccaaa gaaagttacc aaggacctgc ttggagtttt gccggtaagg
    10021 gccagagata taataaagaa gagatatggc cttgagtcta gttcagaggg aatgaccctt
    10081 gaggccatcg gagagacata tggcataaca agagaaagag taagacagat agaaaatttt
    10141 gccttaaatt ccatcagaaa gtccgatgct tatactgcga acgctgtttc tttcgaagag
    10201 ctaaagaagc acatggatga atatggagga gtcgtgcatg agcaggattt tttggatcac
    10261 cttacatccg acaaagaact tcagaatcac gtcagcttct acctcgagct cggtagtgat
    10321 ttcatcaagt tgaaagagga tgaagagttc gctcacagat ggacaataga cgaagagctt
    10381 tccggaaagg ttcacaactc acttagaaaa ctttacgaga gcctctcgga ggatgatctc
    10441 atttcagaaa gcgagatgat tgtaaaattc ctcgatcacc ttgaagagac cgttaaagat
    10501 atcagaaacg aagagctcgc aaagagatgg atcaagatgt ccaagaaaat cggaaagaat
    10561 gcactcggag agtggggaat cgcacattct ccaaacatca gagtaagggg catcagagat
    10621 ctcgcatact tggttttgag aaagcatggt tctcctatgc acttcagaga ggttgcaaag
    10681 gctatcaagc agatatttgg aaaggatgtc aacgaggcga catgccacaa tgagcttata
    10741 aaagacaaga gatttgtgct cgtaggtaga ggtctctatg ctttggcaaa gtggggatat
    10801 agtcagggga cagttcgtga tataataaag actataataa aagataaggg cccacagtcc
    10861 agagatgaga tattgaaaaa ggttttgaga gagagatata tcaaggaaaa tacggttttg
    10921 gtaaatctcc aaaataaaaa atatttcaaa aaggacaaaa acggcaaata tttgatcgta
    10981 tgatccaaga aattttagtc caacttttac aaacctttgg cgaggtgctt tttcgagcat
    11041 tcgctttcgc tctcccgttc ctcgctttca ttatagcttg gagtttatgg agatattaca
    11101 tagtaagaaa ttttatcgct aaggcagagt gggtgatgct ggaggtgaaa ttgccaaagg
    11161 agcaccagaa aactcctcag gccatggaag tcgtcctggg ggcttttcat cagttcccta
    11221 caccgccgac atggtatgac tattggtggc taggcaaggt ccctctttgg ttttctctcg
    11281 agctcgtttc tataaacggc gcggtccact ttttcatgaa aatacagaag agccagagga
    11341 gcataataga gaatcatatt tattctcaat atccacaggc ggaaatacat caggttcctg
    11401 attacgtcgg ggggattaat tttgaggcgc ccgattccaa gtggaaaatg ttcggaatgg
    11461 agcacgtgct tgtaagagag gacgcctatc ctattaagac ttatgttgat tacggccttg
    11521 accgggaact tttgaaggag gaacaaaaag ttgatcccat tacttcgacg atagaatttt
    11581 tgggctcact cggtgaggat gagcaggcat ggatacagat aatcataatg gccgcgcaac
    11641 cgcgatttcc aaaaaaagac acatggttcg gcaaagagga ctggaagggt aatgcaaaag
    11701 agcttctcaa aaaattgtta aagagggatg ccgccccggg atttggcccc atgatgatgc
    11761 tttccccggg agagagggcg actgttgagg cgatagagag gagcatgagc aagctcggtt
    11821 ttgattgcgg aataagaacg ctctattttg gaaaagataa aaagtttaat ccgcaaaata
    11881 ttgcaaatct ttttgccacg ttcaggcagt atggctccgt gaccttaaac agcttcaagc
    11941 ccgtcagaat aaccagcttt aattggtggg tggacccgtt tgggtggaaa ttgaaagaat
    12001 taaaaagctc tctttttaga gcctatgtaa gtagaggata tttttacaga ccattcaaga
    12061 acgtcccgcc gtttacttta aattcagagg agttggcgac tatttaccac ttcccgggcg
    12121 ccgtctctgt aacgccgaca tttagcagaa ttgaatcaaa gagagccgaa gctcctatca
    12181 acctcccgat ctaaagagac tcgcttgatg ctgtcatccc gactttttat ggagggatcc
    12241 cttatactta aagggattgc cacggtctga ggcttaggcg cctcgccctc gcaatgacaa
    12301 taaggagaat acactattta taatttttaa ttcttaattt gtaattgaat atcatgcatc
    12361 ccgatttatc aaatataatg atgttggagt ctgaggaacc gaacgagcct gggggcgaga
    12421 aagccccgtc cccaaaaaat atatttaatc tcaatgagaa ggccagaaag gtcttgttgg
    12481 ctgtatctat tgtcctgatc gtgattctcg cgataaatta caacttcttt gccgcgccgg
    12541 ccgaacatcc gggaggaaca aagtttgcca taacatccgg aatgaccatt tccgaggcgg
    12601 ctgagtattt aagcgagaac aatattataa aatctccgac cgtttttaag acgctcgtat
    12661 ttattatggg cgggagaagt ggggtgatag ccggaagcta ttttatagag caccctgtat
    12721 ctgtttttcg tattgcaatg atggtcacga cgaacggcta cggaataaaa atgtataaag
    12781 tcacaatacc ggaggggctt tcaaataggc agatcgccga tcttctcgcc aaaaatctgg
    12841 acaattttga taaggccaag tttttgaaat tggccaaaga caaggaaggc tatcttttcc
    12901 cggactccta tttctttgaa ccggaatcaa ctcccgaaaa ggttatcgag atgatgaacg
    12961 ataattacaa cgagaaaata aagatggtaa atctggacat cgtcttcttt aataagccga
    13021 taaaagacat tattacgatg gcgtctattc tcgaagagga ggccagaaca gagaatacga
    13081 ggagaatgat agcggatata ttgtggagga ggatagagag aggtatgtcg ttacaggtgg
    13141 atgcgtcctt cgtatatttg cttggcaagg ggagtgcgga gcttactgta gatgatatga
    13201 aaattgactc gccatacaac acctataaat ataaaggatt accgcccgga ccgatcacaa
    13261 accccggcct agcctcaatt ttggccgcca caaagcctct aaagaacaaa tactggttct
    13321 atctgtctga caaggatggg attatgcatt atgcggttac gttcgaggag cataaattga
    13381 ataaagaaag atacttgaga taggccaatt tcttattgtc attgcgagcc gagaccttgt
    13441 cgaggcgtgg caatccctta taattcaaac tcgaagggat ctctccataa agtcgagatg
    13501 acaataaacc ttctcgtgtc attcccgccc ccgatcgcgg tcgagggtaa actccggcgg
    13561 aatccagttt ctcttttgtt ccgatttgaa gtaaaatatt ctctattagc gacgtaggcg
    13621 agagaagcag gaaatttact tttaaaattt tatgcttgcg agcccaggag ctaatataca
    13681 cgctttagcg tcgtctatta ggagaatatt tttatttata cgaaatatga gaatagctat
    13741 taacggtttt ggaagaattg gaagacaatt tttaaagata gcgctagaaa atcaggagct
    13801 ggaagtggtc gcgataaacg atttgggtga tttggataat ctcgcatatt tgctcgagtt
    13861 tgatagcgtt tacagaaatt atgacaagaa agttgagaca aagaatggca acctcgtcgt
    13921 cggcggcaag acaataaaat tccttcagga tccggaacct gcgaaattac catggaggga
    13981 cctcgtcata gacgtggttg ttgaatgcac cggcaggttt acctcgtccg acgccgcaaa
    14041 agtgcatttg gatgcaggag caaagagggt agtcatttcc gcacccgcca aggatgacat
    14101 gacaccgaca tgcacaccga atgtgaacgt aagcgcgctt acagagtcaa agattacttc
    14161 aaacgcgtct tgcacaagca attctattat tcctatcgcc gcaattctcg gcgagaatcc
    14221 gggcattaag ctttccctta taaacacggt tcacggatac acagctgagc agtcgcttgt
    14281 cgatggcccg atgccaaaac ttcacaaaga tatgagaagg ggacgcgccg ccgcgcagaa
    14341 tattgtcccg acaacttcag gtgccgcaag cgcggtaggg aagactatcc gctctatgga
    14401 agggaaattt gacggaatgg cacttcgagt acctgtacct gcaggctcaa ttcttgattt
    14461 cacttttatt tcagagaaaa agacctctgc agaggagata aacgatatcc ttaaagaggc
    14521 ggccgcgaag ccggagtggc aggggataat tacggttacc gacaagccga tagtctcatc
    14581 ggacatcctc ggcaatacgc acggctcaat tgtggatctg aatcttacga gagttgtggg
    14641 cggcgaactc gttaaagtaa tggcatggta tgataatgaa tggggatacg caagtatgct
    14701 agt
//
LOCUS       DAOTEX010000011        14230 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3118
            NODE_2953_length_14230_cov_4.171570, whole genome shotgun sequence.
ACCESSION   DAOTEX010000011 DAOTEX010000000
VERSION     DAOTEX010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868142
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 14230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 883
            CDSs (total)                      :: 833
            Genes (coding)                    :: 829
            CDSs (with protein)               :: 829
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14230
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2953_length_14230_cov_4.171570"
                     /isolate="AMR_MDS_3118"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..661
                     /locus_tag="P5056_01170"
     CDS             <1..661
                     /locus_tag="P5056_01170"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRY62364.1"
                     /translation="SQQLALKQAGTSDEEIRAQEAAVEEARANVSSIRAQIGKTILRS
                     PISGIITKKDFEVGEIVSANEPVLYVISDNNFEMEANVPEADIAKVDIGDAAKVTLDA
                     YGPDVVFDSVVTKIDPAETMIEGVATYKTTLSFVKKDERVKSGMTANIDILTDKKEGV
                     PAVPQRAVSAEDGERFVMLNTGGDTPEKRVIVVGLRGSDGYTEVLSGLVEGDKIVGLA
                     E"
     gene            667..1410
                     /locus_tag="P5056_01175"
     CDS             667..1410
                     /locus_tag="P5056_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015769074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY62365.1"
                     /translation="MKVPKTKNTLIKAEDIVKVYKDEGSETTALRGVSIEINQGDFVA
                     IMGPSGSGKSTLLHILGFLDRQSGGKYYFDGKSMDELSDDEIAKVRNKDMGFVFQAFN
                     LLPRTSVLENVKLPLLYSGIEESKWDELARRAVESVGLTHRIEHESSQLSGGEKQRVA
                     IARALVLEPQIIFADEPTGNLDSKSGQMVMEIIQRLNEEEGHTIILITHETYTAEHAQ
                     RIIHLLDGKIETDRKVEHRHRANDGHFIK"
     gene            1412..2653
                     /locus_tag="P5056_01180"
     CDS             1412..2653
                     /locus_tag="P5056_01180"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY62366.1"
                     /translation="MVFGHIIKTAAKGVSRNKSRSALTILGIVIGITSIMMIMSLGSG
                     AKSLILGQIQGQMGSRVIELRPGRQPKGPTDILAIFSDSLKEKDLEELKKKSNLPHVS
                     GIMPLVFSSASAVYGNETYRSTLYGMNELAPRMYNVVVSDGRFLSDEDVKGFADVAVI
                     GSKVKEELFGTERDVLGKKIKIKGRNLRIIGVLPQKGQGFAFFDDSIIVPYTTAQRYI
                     LGIKYFQHIIIEADTEENVERTLEDITFTMRNSHNITDPEKDDFSMTSQADAIKMLDS
                     VMTILTLFLTAVAAISLVVGGVGIMNIMLVSVTERTREIGLRKALGATERDILLQFMI
                     EAVMLTSIGGIIGIIFGTSLSYITSIILSNFVGLGWEFTFPLFASFLGIGVSGIVGLV
                     FGLYPARQASLKSPIESLRYE"
     gene            2760..3995
                     /locus_tag="P5056_01185"
     CDS             2760..3995
                     /locus_tag="P5056_01185"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY62367.1"
                     /translation="MIIGHTIKTAARGVSRNKSRSALTILGIVIGITAIMMVMSLGQG
                     AQDLILSQMQSVGSKTIIILPGRQATGPSAVVETMSDSLKLKDLLMLRDRSNVPFATR
                     VEPIIMSDGTSSYSNQIFKLSIFGVSGLMQNIFDIKLVSGRFINEEDILNKSPYVVIG
                     SKVKHELFGGDDPLGQKIKIKGVSFRVIGVMPQKGQGFLNFDEAAIIPWSTGQQYVFG
                     TKYFNRIIVEAATEDDVAATTKDIETTLRNSHNISDPEKDDFSVITQEGAKEQISNVL
                     NILTLFLAAIAAISLIVGGVGIMTIMLVSVTERTREIGLRKALGATENDILRQFLIEA
                     VILTLLGGAIGIMLGALSSYAVAFILSNFAGLNWTFSFPFAAAFMGISVSAAVGLIFG
                     LVPARHASLKSPIEALKHE"
     gene            4034..4792
                     /locus_tag="P5056_01190"
     CDS             4034..4792
                     /locus_tag="P5056_01190"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRY62368.1"
                     /translation="MKKDSSWGEVASWYNDVVTGEGSYQRDLILPNLLRLMDIHKGDV
                     VLDLACGQGFFSGEFAKAGASVIASDISPELVEIAKNNPNNKDVDFHVSPAENIDFLK
                     DGSADKISVVLAIQNIEKVQNVFKEVSRVLRTGGKMFLILNHPAFRIPKESSWGYDDE
                     KDIQFRMIDKYLSESKTEIDMNPGENKQKKMTVSFHRPIQYYFKILKNNGFCVGALEE
                     WISNKTSGEGPRKKAEDKSRKEFPLFMALECIKK"
     gene            4904..5128
                     /locus_tag="P5056_01195"
     CDS             4904..5128
                     /locus_tag="P5056_01195"
                     /inference="COORDINATES: protein motif:HMM:NF014633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HRY62369.1"
                     /translation="MKIFIKTKPQSKNVGVEKIDDTHFVVCVKEPPEKGKANEGVRKA
                     LAEYFGVTRSSVVILAGHTAKNKIVEISSL"
     gene            5239..6207
                     /locus_tag="P5056_01200"
     CDS             5239..6207
                     /locus_tag="P5056_01200"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002378.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HRY62370.1"
                     /translation="MHKMRVAVLRGGPSTEYDVSLVTGGNVLRALQNKYHTKDILIDK
                     DGVWHMDGLPTHPEKACRHVDVVFNAMHGEYGEDGKVQQILNLLSVPYTGSGAAASSM
                     AMNKATTKQIFSRHGIKTPMGMVFDSELSPSEIAHSTFRKISPPWIVKPVNGGSSVGT
                     SIARNFEELGRAVINAFGYGNSVLMEEFISGREATCGVIDNFRGQKNYSLLPVEIIKP
                     KHRDFFDYECKYDGTSKEICPGNFDSSAKEEIQNLAMKIHNAMGLRHYSRSDFIVTPR
                     RGIYALEVNTLPGLTPESLFPKSLAAIGCKYEHFLDHLINLALESK"
     gene            6338..6410
                     /locus_tag="P5056_01205"
     tRNA            6338..6410
                     /locus_tag="P5056_01205"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6371..6373,aa:Ala,seq:tgc)
     gene            6941..7147
                     /locus_tag="P5056_01210"
     CDS             6941..7147
                     /locus_tag="P5056_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRY62371.1"
                     /translation="MDKISEQLGKNMKRIRAKKKMSQGDIARALEVDRGYISNIENGK
                     KNPTLATVAKIANALGVSADELLK"
     gene            7150..7875
                     /locus_tag="P5056_01215"
     CDS             7150..7875
                     /locus_tag="P5056_01215"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRY62372.1"
                     /translation="MKNKDFFYLQYNKIDWKNQEKTKINSFINNYIIRNIISNHKGND
                     ISIFDIGFGIGFFFKMLLSEIEGKYKNILIEGCEPSRVNYDYFLKKKPKGLPTGITIN
                     TYKNTFQDIETNNKFDFITAIYVFPHFLSEDLESVVKKVYSMLKNGGKFILVLAKEKY
                     LENKLETEQDLFIEKRNIAYNGKQFKELLHYSDIPKIGKIVDYNREEAYYLDLFQNNN
                     FKVSQKENLEDNGFVCTLFVFEK"
     gene            7879..8313
                     /locus_tag="P5056_01220"
     CDS             7879..8313
                     /locus_tag="P5056_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62373.1"
                     /translation="MTDPIQTILSALTLLGVGGILGGYITYLLDKKKEREFKVLEQKE
                     KRYKSCLLYMDAFFEPKNIKYLSSRQPDIDDATDVIEYLKMEYHEMMLYASKDVIFSV
                     KAFIENPVGDNFLKTILTMRQDLSNRSDLDLSNIILEVKSKS"
     gene            8652..8894
                     /locus_tag="P5056_01225"
     CDS             8652..8894
                     /locus_tag="P5056_01225"
                     /inference="COORDINATES: protein motif:HMM:NF024522.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3977 family protein"
                     /protein_id="HRY62374.1"
                     /translation="MEKIYAEIGFGNDTFLSTEIENGDKEYRIPKFIKPKIIKGYYLR
                     FWVFKTVFILSTNSGFETTKKNRNKLKILFGISGAN"
     gene            9247..10335
                     /locus_tag="P5056_01230"
     CDS             9247..10335
                     /locus_tag="P5056_01230"
                     /inference="COORDINATES: protein motif:HMM:NF022591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2914 domain-containing protein"
                     /protein_id="HRY62375.1"
                     /translation="MAVWEKTKDIYKKYENRLSSVSLIVGFLFDIFVLTRIDMPKENL
                     WIISHLVVAVIGIIIINFYENNDVKRGEVGSVHYWLTVTVQFAFGGLLSTFLVFYFRS
                     ATIYASWPFLLLLALSFACNELLKEHYSRLTFQISYFFLCTFLFTIYYVPILFGSFGT
                     ETFIFSGFVSLGATMILIFFLFHVTMEKFKKSARATFYSVCGLYVVFNLLYFLNIIPP
                     IPLSLKDAGVFHSISRNNQGNYSVQYEWKNLRDYFRPYESFNYAPGETVYVYSAVFSP
                     TNLNTDILHEWQYYDEDAKEWITATKISLSLVGGREGGYRTYSMTRNIFPGYWRVNVE
                     TPSGQVIGRIRFIAREITSTPFLYVGTK"
     gene            complement(10502..10696)
                     /locus_tag="P5056_01235"
     CDS             complement(10502..10696)
                     /locus_tag="P5056_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017416107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HRY62376.1"
                     /translation="MQKLAWILVIVGALNWGLVGVGMFMGANLNLVNLIFGTWPTVEY
                     LVYVLVGIAALIRLPSWKMM"
     gene            complement(10815..11624)
                     /locus_tag="P5056_01240"
     CDS             complement(10815..11624)
                     /locus_tag="P5056_01240"
                     /inference="COORDINATES: protein motif:HMM:NF012449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HRY62377.1"
                     /translation="MDIEHSRAQIIATVGPASGNIDTLKKMIEHQADLFRINFSHGDY
                     DTLAEYISLIKKAAADSEKRIPIIQDLSGPREKTETGHKLDEEAVSIITEKDKRDLYF
                     GLDHDVDYIALSFVEKYQDIIELRGLMEQFGKSKPIIAKIERKEALEKLDGIIEAADA
                     IMVARGDLGENMPMEQLPFIQAMIIKRCKEAKKPVIVATQMMLSMTNSPTPTRAEVTD
                     VANAIIQGADAVMLSEESAIGKYPIETVEMMEKIVLEAERNMAPHLPNTLK"
     gene            complement(11626..12837)
                     /locus_tag="P5056_01245"
     CDS             complement(11626..12837)
                     /locus_tag="P5056_01245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016646118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase"
                     /protein_id="HRY62378.1"
                     /translation="METIRKINAEEILDSRGNPTIRVSVSSENITTFAEVPSGASTGK
                     YEALELRDGDKGRYAGKGVLLAVKNIEEKIAPALMGKDPLNQKGIDETIIELDGTKNK
                     SKLGGNATIGVSMAMARLASKIERKPLFKYLEGISDVKKSRELPYLQANLVNGGKHAE
                     TSLPFQEYHVVPDTEDINEALEIIFKMQNGLKKELHANIGDEGGFVTKFNNIEEPLEK
                     FSKIASKEGLTGKIKYSLDVAASSFFDNGIYDLGYKKIDSTELLNIYKDLCKKYPMLS
                     IEDPFGEDDFTMFTKTEEELPGLFVIGDDLTVTNKERLQEAINKKSVSGLIIKPNQIG
                     TLTETFETMKLAGANGIECIVSHRSGETNDDFIADLAVATGAFGVKFGALQRGERIAK
                     YNRLKEIYSFK"
     gene            complement(13117..>14230)
                     /locus_tag="P5056_01250"
     CDS             complement(13117..>14230)
                     /locus_tag="P5056_01250"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRY62379.1"
                     /translation="SRFLNKDSGLRQAVAGDKPPATLLFIGPIGSGKMHLIKSFTRMM
                     FMGNENAFVRVNCTLFHEGMVTQEKIDQPGFEFFARLEAEESKDKAEKELAEIDEEYL
                     AIEQIFKDKIINGADQDDCVRERDEKVSHIIERQKEVVERHKKFVQSIKWQPGRDYPS
                     VVVIEGIDEMHPGIVNVLKEILDSGRFRNANGEVVYFGNCFIFLKCNLMSEDFTKPQK
                     MGFDTGNADDSFNDLYKAATQKLLRILSMELLKEIGKENIVVFRPFSKKELMDIIDRK
                     LSVLGTIVPPGDDESSVSVVFTDNAKRHIYNEARDRMNKMFGAAPIDRIISLRICNKL
                     DKVIANKEYDVKGGDTLRVDAANGEIVFYKDDKVLA"
BASE COUNT         4016 a         2712 c         3249 g         4253 t
ORIGIN      
        1 ctcacaacag cttgcactca agcaagcagg cacttctgac gaagagatca gggctcaaga
       61 agcggccgtt gaggaggctc gtgcgaatgt gagcagtata agggcgcaga taggcaagac
      121 tatacttaga tcaccaatct ccggaattat taccaagaaa gattttgagg ttggtgaaat
      181 tgtttccgcc aatgagccgg ttttgtatgt gatttctgac aacaattttg agatggaggc
      241 caatgtgccg gaagccgata tcgccaaggt tgatatcgga gacgcggcaa aggttacgct
      301 ggacgcatat gggccggatg ttgtttttga ttctgttgtt acaaaaatag atccggcgga
      361 gacgatgata gaaggagtcg cgacctacaa gacaaccctc agttttgtta agaaggacga
      421 aagggttaag tccggcatga ccgccaatat agatattttg acagataaaa aagaaggagt
      481 cccggctgtt ccacagaggg cggtcagcgc tgaggacggg gaacgttttg taatgttgaa
      541 cacaggtggg gatacgccgg aaaagagagt aatagtcgtg ggtcttcgcg ggtcagacgg
      601 atatacagag gttttgtccg gtctggttga gggagataaa atagtcggtt tggccgaata
      661 actaaaatga aggtaccaaa aactaaaaat actctaatta aagctgaaga tattgtgaag
      721 gtttataaag atgagggctc ggaaaccaca gctcttcgag gtgtctccat tgaaatcaat
      781 caaggtgatt ttgtggctat tatgggacca tcgggctccg gtaagtctac cctgttgcac
      841 atattaggat ttcttgatag gcagtctgga ggaaaatatt attttgacgg taaatcgatg
      901 gatgaattat ccgatgatga aattgcgaag gttaggaaca aggacatggg ttttgtcttt
      961 caggcgttta atttgttgcc gaggacttct gtgctggaaa atgtaaaact gccgcttctt
     1021 tattcgggga tagaagaatc taagtgggat gagcttgcaa gacgtgccgt agagagtgtg
     1081 ggccttactc atagaataga acacgaatcc tctcagcttt ccggaggaga gaaacaaaga
     1141 gtcgcaatag cgagagctct cgtgcttgaa cctcagatta tctttgccga tgaacctacc
     1201 ggtaaccttg attccaagtc aggacaaatg gtaatggaga taattcagcg gcttaacgaa
     1261 gaggaaggac atacaatcat tctcattact cacgaaacat atacggctga gcatgcgcaa
     1321 agaataattc atctcctaga cggcaagata gagacagaca ggaaagttga acacagacac
     1381 agggcgaatg acggtcattt tataaaataa aatggttttc ggtcatataa taaaaacagc
     1441 cgcaaagggt gtctctagaa acaaatcgcg ttccgcgcta acgattctcg ggatcgttat
     1501 tggtattact tctatcatga tgatcatgtc tttggggagc ggtgccaaga gtcttatctt
     1561 aggacaaatc caggggcaaa tgggatctcg ggtgatagag cttaggccgg gccgacagcc
     1621 gaaggggccg accgatatat tggccatttt ttccgactca ctaaaagaaa aagatttgga
     1681 ggagctgaag aaaaagagca acctcccgca tgtctcaggt attatgccgc tggtttttag
     1741 ttctgctagc gctgtatacg gcaacgagac gtatagatca actctatatg gtatgaatga
     1801 attggcgccg cgaatgtata atgtggtggt ctcagacgga agatttttga gcgatgagga
     1861 tgttaagggt tttgccgatg ttgcggttat cggttctaag gtgaaggagg agctttttgg
     1921 aacagaaagg gatgttttag gcaaaaaaat aaaaattaag ggtagaaatt taagaattat
     1981 cggcgtcctt cctcaaaaag gtcaggggtt tgcatttttt gacgattcaa ttattgtccc
     2041 atacacaact gctcagcgat atatattggg cataaaatat tttcagcaca ttattattga
     2101 ggccgacaca gaagagaatg tcgagaggac tcttgaggat ataactttca cgatgagaaa
     2161 ctcccacaac attacagacc cggagaaaga tgattttagt atgacgagtc aggcggacgc
     2221 tataaaaatg ttggattctg ttatgacaat tttaactcta tttctgacgg cagttgccgc
     2281 gatatctttg gtcgtgggag gcgtcggaat tatgaacata atgctcgttt ctgttacaga
     2341 gagaacaaga gagattggtc tccgaaaggc gcttggcgcc accgaaagag atatattgtt
     2401 gcagtttatg attgaagcgg taatgcttac aagtatcggc ggaataatcg gtataatatt
     2461 tggaaccagc ctttcttata taacatcgat tattttaagt aattttgtcg ggctcggttg
     2521 ggagtttacc ttcccccttt tcgcctcctt tttgggtata ggggtttccg gtatcgttgg
     2581 tctcgttttt ggactttatc cggcaagaca agcatcatta aagagcccca tcgaatcctt
     2641 aagatatgag taaaaagtta tattttttac agcctgagtc cgacttgccc cgtagcgagc
     2701 cgcgctctgc tactggggtt gaagcccttc gttacgaata aataatttag aaaaacttaa
     2761 tgattatcgg gcacactata aaaacagccg caagaggtgt ctctagaaac aaatcacgtt
     2821 ccgcgttaac cattctcggg attgttatcg gcataaccgc cataatgatg gttatgtctt
     2881 tgggccaggg cgcccaagat ttaatattga gtcaaatgca gtcggtcggg tctaagacaa
     2941 ttattattct ccccggtcgc caggctaccg gcccctctgc tgtcgtagaa acgatgagcg
     3001 attctcttaa gctgaaagat ctgttaatgc ttagagatag atcaaatgtt ccattcgcta
     3061 ccagagttga gccgatcatt atgagcgacg gcacctcctc ttattcgaat caaatattta
     3121 aattaagtat atttggagtc tccgggctta tgcagaatat ctttgatatt aagttggtgt
     3181 ccggtcggtt tattaacgaa gaagatattt taaacaaatc gccttatgtc gtaatcggct
     3241 caaaggttaa gcacgagctt tttgggggag atgatcctct gggtcaaaaa ataaaaataa
     3301 aaggcgttag ctttagggtt ataggagtaa tgcctcaaaa aggtcagggc tttcttaact
     3361 ttgatgaagc ggccataata ccatggtcaa ccggccagca atatgttttt ggcacaaaat
     3421 attttaatag gatcatcgta gaagcggcta cggaagatga cgttgccgct acgacaaaag
     3481 atatagagac aactttgcgt aattctcata atatttctga cccggaaaaa gatgactttt
     3541 cagttattac gcaggagggg gcaaaggagc agataagtaa tgtgttaaat atattaacat
     3601 tgtttttggc tgccatagcg gcgatttctt taattgtcgg tggagttgga attatgacga
     3661 taatgctggt ttcggttaca gaaagaacta gagagattgg tctcagaaaa gcgcttggcg
     3721 ccacagaaaa tgatattcta agacaatttt tgattgaggc cgtgatttta acattgcttg
     3781 gtggagctat aggcattatg ctcggcgcct tatcgtcata tgcggtcgcc tttatattga
     3841 gtaattttgc cggattgaat tggacatttt cgtttccttt tgccgcggca ttcatgggga
     3901 ttagcgtttc ggctgcggtg ggtctgattt tcggattggt tcccgcaaga cacgcctcgc
     3961 tcaaaagtcc aattgaagcc ttgaagcatg agtaaattcg cttattgata tattttttgc
     4021 tatcctaatt tttatgaaaa aagacagttc ttggggagag gttgcttctt ggtataacga
     4081 tgtcgttacg ggcgagggga gttaccagag agatttgata ttgccgaacc ttctcaggct
     4141 tatggatata cataagggag acgtggtttt agatctcgca tgcggccaag gttttttctc
     4201 cggagaattt gctaaagccg gggcttctgt aatcgcttca gacatttcgc cggagttggt
     4261 agagatagca aaaaataatc caaataataa ggatgtggat tttcatgttt ctccggctga
     4321 gaatatcgat tttctgaaag atggtagtgc ggacaaaata tctgtcgttc tcgcgataca
     4381 gaatatagaa aaagttcaga acgtttttaa ggaagtatct agagttttga gaaccggtgg
     4441 aaaaatgttt ttaattttaa atcaccccgc ctttagaata cctaaagaaa gctcgtgggg
     4501 atatgatgat gaaaaagata tccagtttag gatgatagac aagtatcttt ccgaatcaaa
     4561 gacagagata gatatgaatc ctggagaaaa taaacaaaag aagatgacgg tttcttttca
     4621 cagacctatc cagtattatt tcaagattct aaaaaataac ggattttgtg tgggtgctct
     4681 ggaagagtgg atatcgaaca agacaagcgg ggaggggccg agaaaaaagg ccgaggataa
     4741 atccagaaaa gaattcccgc tatttatggc gttggaatgt ataaaaaagt aaggacttgc
     4801 ccgccaaaat ttctcaccgc agaaactttg ttggtattgt agtgttaagc gtcaaccaga
     4861 tataaaatat agtaaaatat tatttgttaa gaaatttagg agggtgaaaa tatttataaa
     4921 aacaaagcct cagtccaaga atgttggtgt cgaaaaaata gacgacacac attttgtggt
     4981 gtgcgtaaaa gaacctccgg aaaaggggaa ggccaacgaa ggggtgagaa aggcgctcgc
     5041 tgaatatttt ggggttacgc gctcttctgt ggtgatactt gccggccata cggccaaaaa
     5101 taaaatagtg gaaatcagtt cgctctaaat agccgtcatt gcgagccgaa ggcgtggcaa
     5161 tccagatctt ttgtggtgga aactttacaa cttgataaca ttataacttt ataactaatt
     5221 ttttggtata atgtatctat gcacaagatg cgtgttgctg tattgcgtgg cggtcctagc
     5281 accgaatatg acgtctctct tgtaacagga gggaatgttt tgcgcgcact gcagaataaa
     5341 tatcatacta aggatatctt gatagataag gatggtgtgt ggcacatgga tggacttcct
     5401 actcatccgg aaaaggcctg tagacatgta gatgtggtct ttaacgccat gcatggggag
     5461 tatggagaag acggcaaggt ccagcagata ttgaatttac tctcggttcc ttatacaggt
     5521 tcgggcgcgg ccgcatcctc tatggcgatg aataaggcga ccactaagca gatattttcc
     5581 agacacggca ttaaaacacc catgggcatg gtttttgata gtgagctcag tccttcggag
     5641 atagcgcatt ctacttttag gaaaatatct ccgccgtgga tagtaaaacc ggtgaacgga
     5701 ggctcatctg tgggcacatc tatcgctagg aattttgaag agctcggccg agccgttata
     5761 aatgcgtttg gatatggtaa tagcgtgctg atggaggagt ttataagtgg aagggaggcg
     5821 acctgcggag tgatagataa tttcaggggt caaaagaatt attcgctctt gccggtagaa
     5881 ataataaaac ctaaacacag agactttttt gattatgagt gtaaatatga cggaacttca
     5941 aaagagatat gccccgggaa ttttgatagt agcgctaaag aagaaattca aaatcttgcg
     6001 atgaagatac acaacgcgat gggtcttagg cattattcta ggtctgattt tattgtgact
     6061 ccaagaaggg gaatatatgc cctggaggtt aatactttac cgggcctcac tcccgagtct
     6121 cttttcccaa aatctcttgc ggccattggt tgtaaatatg aacatttctt agaccatctt
     6181 ataaaccttg cgttagaatc aaaataatgt ttatatagta gttggaaagt aaataaaaga
     6241 aggaggaagg tttctgtggt cattgcgagg gcacagcccg tggcaatcca tgtttgtaaa
     6301 ttcgtaattg ataattatta atttataatt tatttctggg ccgttagctc atctggtaga
     6361 gcaccacatt tgcaatgtgg gggtggcagg ttcgagtcct gtacggtcca ctgtttaaaa
     6421 acattagcgc ccttgtgggg cgcttttgtt ttacagtgga ccgtaagcaa agcgcctgcg
     6481 cgctttgcgt caggactcga aaaggttctc cgttatattt tagaaatttt aatttctaaa
     6541 atatcggaaa cctatactgt tcccgtaggg aaagagtcct gttctgcgaa agcagttatt
     6601 ttttattttt gtggtgtcat tatagcattt gcgcgaactt ttttcgaacg aaatccctag
     6661 cacattccgc agggtaaagc cctcacgcgc tacgcgcgtg ttctcgcaag gcgagaaagc
     6721 ggaaatgtgc aaacgcgctc cgccacatat tttctgggga tgtggaggcg gggcgatgga
     6781 ctcgcccgcg cggaggaggg gtctggggag gaatccgcgc gggcttcggc tttctttttt
     6841 tgaaacggcg gcggctagaa aagaagaaaa taccatagtg tattttagta taacattgtc
     6901 tgtaaatata acaaagtgct agaatgtgaa taatctcaat atggacaaga tttcagaaca
     6961 attaggtaaa aatatgaagc gtattagagc taaaaagaaa atgtcgcaag gcgatattgc
     7021 tcgcgcctta gaagttgata gaggctatat cagcaatatt gagaatggaa agaaaaaccc
     7081 aacccttgcg acagttgcta aaatagctaa tgcgcttggt gtatcggctg atgagctttt
     7141 gaaataatta tgaaaaacaa agatttcttt tatctacaat acaacaagat tgattggaaa
     7201 aatcaggaaa agacgaaaat aaattcgttc atcaacaact atattatccg aaatataatt
     7261 tcaaatcaca aaggcaacga catttcaatt tttgatattg gtttcggtat tggctttttc
     7321 ttcaaaatgc ttctttctga aatcgaagga aaatataaaa atattctcat agaaggttgt
     7381 gagccatcgc gcgtaaatta tgattatttt ttgaagaaaa agccgaaagg acttcctact
     7441 ggaattacaa tcaacactta caaaaacact ttccaagata tcgaaaccaa taacaagttt
     7501 gatttcatta ctgcaatata tgttttcccg cactttcttt ccgaggattt ggaaagtgtg
     7561 gtgaaaaaag tttactcaat gcttaaaaat ggtggtaaat tcattttagt gcttgccaag
     7621 gaaaagtatc tggaaaataa acttgaaact gaacaagatt tatttattga gaaaagaaat
     7681 attgcttaca acggcaagca gtttaaggaa ttactgcatt acagcgatat tccaaaaatc
     7741 ggaaaaattg tcgattacaa ccgagaagaa gcatattatc ttgatttatt ccaaaataac
     7801 aatttcaagg taagccaaaa agaaaatctg gaagataatg gatttgtttg cacattgttt
     7861 gtgtttgaaa aataatctat gaccgaccca attcaaacaa ttttatctgc tctaacactt
     7921 ctcggcgtag gtggtattct cggcggatat atcacttatc tactcgacaa gaaaaaggaa
     7981 cgagagttta aggtgttaga gcagaaagaa aaaaggtaca aatcctgcct cctgtacatg
     8041 gacgctttct ttgagccgaa aaatataaaa tatctttcga gccgacaacc cgatattgat
     8101 gacgctactg atgttatcga atacctgaaa atggaatatc acgaaatgat gctctatgcc
     8161 tcgaaagatg taattttttc ggtgaaagcg tttatcgaaa atcctgtcgg cgataatttt
     8221 ctcaaaacga ttttgacgat gaggcaggat ttgtccaata ggagtgattt ggatttgagc
     8281 aatattattt tagaggtcaa atccaaatca tagccgccgc cgtttcaaaa aaagaaagcc
     8341 gaagcccgcg cggattcctc cccagacccc tcctccgcgc gggcgagtcc atcgccccgc
     8401 ctcctttgcc ccagaaaaat agtggcgggg cgcatcagga gttccgttca aagaaagttc
     8461 gcgcaaatgc tataatgaca ccaccgtaag taaagcgaag ctttacggcg gattcgaacg
     8521 gattgtcggt cacgagctca gcagagcttg tcgacaatcc ggtgcccaga aaattttaat
     8581 tcgacggaat tgaaattttc gagggagaaa ttccgctacg gtccaccaga cctggtttcg
     8641 ttagaactaa tatggaaaaa atatatgcag aaatagggtt tggcaatgat acttttttat
     8701 ccaccgaaat agaaaacggc gacaaagagt atcgtattcc taagtttatt aaacctaaaa
     8761 tcataaaggg ttattatctt cgtttttggg tttttaagac agtatttata ttatcaacta
     8821 actcgggatt cgaaactaca aaaaagaata gaaataaatt aaagatattg ttcggtataa
     8881 gtggggctaa ctaattttag ttcttgcatg attaggttcc aattcacgcg gatgtagtcc
     8941 actgtttaaa aacattagcg cccttgtggg gcgcttttgt tttacagtgg accgtaagca
     9001 aagcatctta aatgctttgc gccaggactc gaaaggcttt tgagcatatt ttgaagtgcg
     9061 aagcacgaac aaaatatcca aaaggcatac tgccgccgta ggcggagagt cctgttctgc
     9121 gaaagcagcg ggcgattata tttttgtagg accgtacagg actcgaaggc cggaagcggg
     9181 cccctttctg caagaaaggg agccgcccca cttggggcgg ttttttattt gttgtattat
     9241 atatgcatgg ctgtctggga aaaaactaaa gatatatata aaaaatacga gaacagattg
     9301 tcttcagtgt ctctgattgt gggattcttg tttgatattt ttgtgctgac aaggattgat
     9361 atgccgaaag aaaatctttg gattatttct caccttgtcg tggcggtaat tgggattatc
     9421 atcataaatt tttatgagaa caatgatgta aaaagagggg aggttggtag cgtccactac
     9481 tggcttacag ttactgttca attcgctttt ggagggcttc tctctacttt tttggtcttt
     9541 tattttagga gcgcaacgat ttatgctagc tggccgttcc ttttacttct cgctctttct
     9601 tttgcgtgca atgaacttct aaaagaacat tattccagat taactttcca gataagttat
     9661 ttttttctct gcacattcct ttttactatt tattacgtgc cgattttatt tggtagcttt
     9721 ggaaccgaga cttttatttt tagcggattt gtaagccttg gggccacaat gatacttatt
     9781 ttctttcttt tccacgttac aatggagaag tttaagaaaa gcgcgcgggc cacgttttat
     9841 agcgtatgtg gtttgtatgt ggtttttaat cttctttatt ttttaaatat tattcctcct
     9901 atccctcttt cgttaaagga tgcgggcgta tttcattcca tatctagaaa taatcaaggc
     9961 aactacagcg tccaatatga atggaaaaat ctaagagatt atttcaggcc gtatgaatct
    10021 tttaattatg cccccgggga aactgtttat gtatatagcg cggtcttttc tcctacgaat
    10081 ttaaacaccg acattcttca tgaatggcaa tattatgacg aagatgcgaa ggagtggata
    10141 acggccacaa agatctcgct gtcgcttgtg ggaggcaggg agggcggata taggacatat
    10201 tctatgacgc gaaatatctt tccgggatat tggcgggtga atgttgagac gccgtcgggg
    10261 caggttattg gaaggataag atttatcgcg agagaaatca cttcgactcc ttttctttat
    10321 gtaggcacga agtagacaaa atttattaaa atatttatac acaatcaggg aaatgaagtg
    10381 aaatagttct tttgttatcc cgcggccacg tgctgcggga tttttattta ccctgtttag
    10441 taaaaattaa caaggtaaat aaaaaactcc gcccgtaagc ggagcctttt attttaaaaa
    10501 attacatcat tttccaagaa ggaagtctga tgagggctgc aatacctacg agaacgtata
    10561 cgaggtattc aactgttggc catgtgccaa agatgaggtt tacgaggtta aggtttgctc
    10621 ccatgaacat accaactcca accaatcccc agttgagggc tcctacgatc acaaggatcc
    10681 atgctaattt ctgcattttt tatatttaaa attaaaggaa taattcgacc tagaaccgtt
    10741 aagttcttaa gtataattat accgctttta gtagatgtgt ggtctaattt tatccacaga
    10801 tttgggataa tgccttattt taaggtattc gggagatgcg gggccatgtt tctttcagct
    10861 tccaggacta ttttttccat catttctact gtttctatcg gatatttgcc gatagctgat
    10921 tcttccgaaa gcataactgc gtcagcaccc tgaataatag cgttcgccac atcggtcacc
    10981 tcggccctag tcggtgtggg ggaatttgtc attgagagca tcatctgggt ggctacgatg
    11041 accggttttt tagcttcttt gcatctttta attatcatgg cctgaatgaa tggcaattgc
    11101 tccattggca tattttctcc caagtcgccc cttgccacca tgatggcgtc cgcggcctca
    11161 ataatcccgt ctaatttttc tagagcctct tttctctcaa tttttgcgat tatcggtttg
    11221 cttttcccaa attgttccat gaggccacga agttctatta tatcctgata tttttccaca
    11281 aaagaaagag ctatataatc cacgtcatgg tccagaccaa aataaagatc gcgtttatcc
    11341 ttttccgtta ttatagaaac ggcttcttca tctagcttat ggccggtttc tgttttttct
    11401 cggggtcctg acaagtcttg tattatgggg attctttttt cgctatccgc cgcagctttt
    11461 tttatcagag aaatatattc tgcgagcgta tcatagtcgc cgtgcgaaaa gtttatccta
    11521 aataagtcgg cctgatgctc aatcattttt ttaagcgtat caatattgcc gcttgcgggt
    11581 ccgacagttg cgattatctg cgctcttgag tgttctatgt ccatgttatt tgaaggaata
    11641 aatttctttt aatcgattat attttgcgat acgctctcct ctttgaagcg ctccgaactt
    11701 aactccaaag gcacccgtgg caacagcgag gtccgcgatg aaatcatcgt tcgtctctcc
    11761 gctcctgtga cttacaatac actctatacc gtttgctccg gccaatttca tcgtctcgaa
    11821 agtttccgtg agggtgccga tttggttcgg cttgattatg aggcccgaaa cacttttttt
    11881 atttatggcc tcctgtagtc tttctttgtt cgtgacggtt aggtcatctc caatcacaaa
    11941 tagtcccgga agttcctcct cggtttttgt gaacattgta aaatcgtctt caccgaaagg
    12001 atcttcgata gaaagcatcg gatatttctt gcagaggtct ttgtaaatgt ttagaagctc
    12061 ggtcgaatct atttttttat atcccagatc ataaattccg ttatcaaaga aagatgatgc
    12121 cgctacatcc agagagtatt ttattttgcc cgtaagtcct tcctttgaag caatttttga
    12181 gaatttctca aggggttctt ctatattatt aaatttagta acaaatccgc cttcgtctcc
    12241 tatgttcgcg tggagctctt ttttgaggcc gttttgcatt ttgaaaatta tttcaagagc
    12301 ttcatttata tcttctgtat ccggcacaac atgatattct tggaacggca gggaggtctc
    12361 tgcgtgcttc cctccattca ccaggttcgc ttgtaaatat ggcagttccc tcgatttttt
    12421 aacgtcactg ataccttcta aatatttaaa aagaggcttt cgctcgatct ttgaggccaa
    12481 acgagccatg gccatactca caccgattgt tgcgttgccc ccaagctttg atttgttttt
    12541 tgttccgtct agctcgatta tcgtttcgtc tatacctttt tgattaagag gatctttgcc
    12601 cataagcgcc ggcgctatct tttcttcaat atttttgacc gctagaagaa ctcccttgcc
    12661 ggcatatctt cctttgtcgc cgtctctaag ctctagcgct tcgtatttgc ccgtgcttgc
    12721 tccggacggg acctccgcaa aagttgtaat gttctcggag gaaacagaaa ctcttatcgt
    12781 aggattgcct cttgagtcca atatttcctc ggcatttatt tttcttattg tttccattta
    12841 ccttattaag taaaatttaa ttaatatttt aataataaca tatataaata ttaaataaat
    12901 tttaaatagc ccgttgggaa cgggctattt tacttatata gggtgacgac cccagtgaaa
    12961 taggggcggt tttgttgtct ctccacgagg tctgagtaag ggggttattg atgattaaaa
    13021 gtgataaatt aggcttgatt tcacggggtt catcctgtcg attttttgat aaaaaccgac
    13081 aggatgaaaa aaggaggccg tttggcctcc ctatactcac gctaacactt tgtcgtcttt
    13141 gtaaaagacg atctctccgt ttgccgcatc gactctcaac gtgtcgccgc ccttcacgtc
    13201 gtattccttg ttggcgatga ccttgtcgag tttgttgcag atgcgaagtg agataattct
    13261 gtcgatgggc gccgcgccga acatcttgtt catgcggtct ctcgcctcat tatagatgtg
    13321 acgcttggcg ttgtctgtga aaacgacgct tacagaagac tcgtcatctc ccgggggaac
    13381 aatcgttccc agcacagaaa gttttctgtc gatgatgtcc ataagttcct ttttggagaa
    13441 tggccggaag acaacgatgt tctctttacc gatttctttc aggagctcca tcgacaagat
    13501 ccttaagagc ttttgggtag cggccttgta aagatcgttg aaagagtcgt cggcgttacc
    13561 ggtatcaaaa cccatctttt ggggtttcgt gaagtcttcc gacatgaggt tgcattttaa
    13621 gaaaatgaag cagtttccga agtagacaac ttcgccgttc gcatttcgga acctaccgct
    13681 atcgagaatt tcttttagca cattcacaat tccgggatgc atctcgtcaa tgccctcgat
    13741 gacaacaacg gaaggatagt ctcttcccgg ttgccatttg atgctctgca cgaatttttt
    13801 gtgtctctcc acgacctctt tttgtctttc gataatgtga cttactttct cgtcgcgctc
    13861 tcttacgcag tcatcctgat cggcgccgtt tataatctta tctttgaaga tttgctcgat
    13921 tgcaagatat tcttcgtcaa tctcggcgag ttctttctca gccttgtctt tggactcttc
    13981 agcctcaagc cgagcaaaaa attcaaagcc gggttgatca atcttttcct gagtaaccat
    14041 gccctcgtga aagagcgtgc agttgactct gacgaacgca ttttcgtttc ccataaacat
    14101 cattctggtg aatgatttga taaggtgcat tttgccggat ccgatagggc cgatgaagag
    14161 tagcgtcgcc ggaggtttgt cgccggcaac ggcctgtcga agtccgctgt ccttgttgag
    14221 aaaacgggac
//
LOCUS       DAOTEX010000012        14076 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3118
            NODE_2994_length_14076_cov_4.029028, whole genome shotgun sequence.
ACCESSION   DAOTEX010000012 DAOTEX010000000
VERSION     DAOTEX010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868142
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 14076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 883
            CDSs (total)                      :: 833
            Genes (coding)                    :: 829
            CDSs (with protein)               :: 829
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14076
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2994_length_14076_cov_4.029028"
                     /isolate="AMR_MDS_3118"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..843
                     /locus_tag="P5056_01255"
     CDS             <1..843
                     /locus_tag="P5056_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005961114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HRY62380.1"
                     /translation="RKAAKAAKDSVLRKGVLDGLSLPGKLADCQTKNPEEAEIFIVEG
                     DSAGGSSKQGRDRRTQAVLPLKGKILNVERTRLDKVLSSNEIKALVIAMGTAIGDIFD
                     QTKLRYGKIIIATDADVDGAHIRTLLLTLFYRYFQPLVSAGNIYIAQPPLYKIKKGRD
                     VSYAYSDEEKEQIVKSFGAGVAVEQESEGGSVGEEGAEEETGETIKKSTKVSIQRYKG
                     LGEMNPEELWETTMDPERRILKQVTIDSAIEADKTFDVLMGSEVLPRKMYIQSHAKMA
                     NIDA"
     gene            878..1708
                     /locus_tag="P5056_01260"
     CDS             878..1708
                     /locus_tag="P5056_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62381.1"
                     /translation="MTYKNVKILDFTLSFLFLIFYISILATPLISFADTLSNVGIIKS
                     GIWYSKDPFYAGDKIRIYTLVFNGGEYDLLGDLTFDDNGKVICTGNFSAASGRTQELW
                     CDWTATIGTHKISAKIINPKVSPIGESPRTIILENNVSGVSEKKVTLPPQKEEESLVS
                     VPRIASSSATSTSTLIEKGLDSFKDIVLQVLPEKITSQDVVSQGDAVASATPKTIREG
                     VGKVMEKIGAEKLKTPVMFIVDFLVVVYNFLMRQPVVLIALLSFIVWKFAKYLYGRLV
                     "
     gene            1714..2922
                     /gene="tgt"
                     /locus_tag="P5056_01265"
     CDS             1714..2922
                     /gene="tgt"
                     /locus_tag="P5056_01265"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015641217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HRY62382.1"
                     /translation="MGSNLNFKIEKKLPGRLGRVGVLETPHGKIETPAFIVVGTKATV
                     KALSPEDVQTLGAQAVLSNTYHLYLEPGDELVKNAGGLHKFMNWSGPTFTDSGGFQVF
                     SLGVAMEEKANKFGAKKQETVNENICKEEGNTKLSKIDEDGVTFRSHIDGTSHRFTPE
                     RSMEIQHNLGADIIFAFDECTSPLASREYQIEAMKRTHRWAKRSLDRHNELGGPQSLF
                     GIVQGGRFEDLRKESAKEISSLLFDGFGIGGSFDKEDVYKALVWVNEILPENKPRHFL
                     GIGAVEDLFGGIENGVDTFDCVAPTRMARNGALLTKEGRMNIMNAKYKTDFTPIEGDC
                     ECYTCKNYTRAYLAHLFRTEELFAYRLSSIHNLYFVVNLVKKIRQSILDDKFFEFKEQ
                     FLNRYNSIKN"
     gene            complement(2980..3720)
                     /locus_tag="P5056_01270"
     CDS             complement(2980..3720)
                     /locus_tag="P5056_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62383.1"
                     /translation="MNENETPLTTTSVTQTAHEISRSENGYKTEFATFTKGGESTGRH
                     TKRMKNGMFLGQTGIGANHVNNIFEPLPPQNTWTKGKKQTVARNYDGTFQFGEKDVLI
                     PPRHTPTAIKVADVAITFHTCTPTGLKQIDKIVEFLNYKVAYARIDAAREVDTKIQEA
                     LSNNVENFTLNREDEIKKAEKKAVVAGLEAMVIEKPPTMRKKPPKAPRTKRIRVEDED
                     GESKKHHGKKGGGKKNGKKGGGKGKGKK"
     gene            complement(3954..4817)
                     /locus_tag="P5056_01275"
     CDS             complement(3954..4817)
                     /locus_tag="P5056_01275"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009892160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRY62384.1"
                     /translation="MLIANNWKDYELLDSGDGEKLERWGSVVLSRPDPQAFWPKSLSD
                     KEWRNADGIYTRSEGGGGEWKFKEGTPKKWEIKYGDLKFSIKPTPFKHTGLFPEQSAN
                     WDWMKEKIKGAHREISVLNLFAYTGGATVAASNAGAKVCHVDSSNGIVTWAKENIELN
                     NKSGEVRWIIDDALKFVDREIKRGVKYDGIIMDPPSYGRGAKGEVWKLEEKLWPLVDK
                     TSRLLSDNPLFFIINSYTTGMSPTVIANILKTAFKDKGGKIETDELGLKEHGGDRILP
                     QGVFGRWSKMS"
     gene            complement(4822..4892)
                     /locus_tag="P5056_01280"
     tRNA            complement(4822..4892)
                     /locus_tag="P5056_01280"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4858..4860),aa:Trp,seq:cca)
     gene            complement(5001..5159)
                     /gene="rpmG"
                     /locus_tag="P5056_01285"
     CDS             complement(5001..5159)
                     /gene="rpmG"
                     /locus_tag="P5056_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HRY62385.1"
                     /translation="MPQKSLIKLACTSCKRINYWSRKNKKLVERKIELLKFCKWCKKK
                     TKHKEIKK"
     gene            5475..6815
                     /gene="xseA"
                     /locus_tag="P5056_01290"
     CDS             5475..6815
                     /gene="xseA"
                     /locus_tag="P5056_01290"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HRY62386.1"
                     /translation="MPKKKKDKIQNGDDGSLGDLFELPKGGLPISPAKDYFSVGEFLD
                     ILNEHLKSEKVSVAGEVTEFRAHDKWVSFSLKDKEDKSILKCFLSIWTYRSAGVLLED
                     GMEVKVSGYPRIYKLAGTFSFNVETIEPLGEGSLKKAYLLLLKMLEKEGLFARKRALP
                     RFIKNIGVISSKDGVVIHDFRKNLKPLGFQIKFFNSRVEGADAVPGIIGGIKWLNKNM
                     PDLDVMVIMRGGGSLESMQAFNNEQVCREIFSSKIPVLCGIGHEVDVPIACLVADAYV
                     STPTATAYTINESWDDLVSGLPMFERRIITQFGFYLKEFSSKLPYLWKNIYGRFGDNL
                     AGLKKEASGISGRIFSAFRRVFVQFGIMSDRITKDAIRIIGDRLTHYISQIQKFEKII
                     AVADPERNLRLGYSIVFDGSGRVIKNAKDVKKGEVIKTKLGKGEIESEVKSITN"
     gene            6848..7060
                     /locus_tag="P5056_01295"
     CDS             6848..7060
                     /locus_tag="P5056_01295"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:NF014648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HRY62387.1"
                     /translation="MATKNNDIGESLKKLEAIASWFEKENEIDVEEGLKKVREGAALI
                     KDLKSRLAEVTNEFEEIKKELTKDTE"
     gene            complement(7119..8030)
                     /gene="miaA"
                     /locus_tag="P5056_01300"
     CDS             complement(7119..8030)
                     /gene="miaA"
                     /locus_tag="P5056_01300"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HRY62388.1"
                     /translation="MKEKHKSKDKLIAVVGPTSSGKSDVAVLIAKKIGGEIISADSRQ
                     VYKGMDIGTGKITKKEMAGVPHYLLDVANPKKIFTVADYQKMARKAVEKILQKGKVPI
                     LCGGTGFYIRAIIDDLEIPDVPPNKKLRKELAGKNADELFAILKKMDKRRAEKIDRHN
                     PVRLIRAIEIAQALGKVPTLKNKAPKYEILEIGLYPGDKILKERINLRLLKRIKAGMI
                     AEIKKLHKEGLSFARMKDMGLEYRYVARYLQGLINKEEMISELNKEIEHYAKRQMTWF
                     RKDKRVKWFKPNQIEKIIKTAEKFCRG"
     gene            8051..8752
                     /locus_tag="P5056_01305"
     CDS             8051..8752
                     /locus_tag="P5056_01305"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRY62389.1"
                     /translation="MKEKSNSKTFWNKEYKTPGIFNLSTEPAEDLLKFTRWLEREYGK
                     GFLDQRSFVLDLGCGNGRNIIYLARTFGCKGAGYDISDEAIEQAKKASAGLHIQFTAR
                     MIEGKIDLEDESVDVVLDMMASHYLREKDRDIYLKEITRVLKPGGWLLLKSFLKEEDR
                     HTERLLRDNPAGEDDAYIHPEHGTYEHVWAEEKMRAFYGEAFDIDKVERSGKHLMKGK
                     AFKRRHFVAYLRKRY"
     gene            8884..9087
                     /locus_tag="P5056_01310"
     CDS             8884..9087
                     /locus_tag="P5056_01310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62390.1"
                     /translation="MKFRLQNRKVDIMLCPLCNAEMHKNPKVSPRNKHLSLKCPRCSF
                     VIFYKGVESMKNLDPEPEPKEKK"
     gene            9193..10119
                     /locus_tag="P5056_01315"
     CDS             9193..10119
                     /locus_tag="P5056_01315"
                     /inference="COORDINATES: protein motif:HMM:NF013832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62391.1"
                     /translation="MIITYIILLVLLFYVLGASADMVVRRIKQIADKFKISILSMGMV
                     LGALTTLPEFFVGVNSLVLDVPNLYFGTLMGGIIVVFCLILGFSLVLNRKVKTDGKYS
                     SILPVSLALLLPLFMGLKGTLNYLDGIIFLGIYLIFLYKNTIEHNHINVSDITKNEVV
                     LAKNFFWIITGVILVVFSSNLIVVIATRILAAYNVSALMIGLIVFSLGTNLPELIIAI
                     RSWTRHVPELSLNHIIGSALANILVLGFMLLVRPLSFDIGASYKFLLTVMCIVLFTLT
                     SFYKSDGELTRKEGYKLLVIYLVFIVGQILFS"
     gene            10136..10531
                     /locus_tag="P5056_01320"
     CDS             10136..10531
                     /locus_tag="P5056_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013902087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HRY62392.1"
                     /translation="MQAYNWYSTLIKPSFAPPAWVFGPVWTVLYILIAISFSYVFYLY
                     IKGRISFWILLPFILNLVFNFAFTPVQFGLQNNLLASIDVVAVIGTLLYALYAIYPYA
                     AWVVYANIPYLLWGAFATVLQLTITYLNM"
     gene            10557..10961
                     /locus_tag="P5056_01325"
     CDS             10557..10961
                     /locus_tag="P5056_01325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000675692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HRY62393.1"
                     /translation="MKGYVSNIEKESLENDNFRKVLYTAKYSQLVLMSLLPKEEIGEE
                     VHSDVDQFFRIEKGTGVVIIDGVTHNISDGFAIIVPSGAKHNVINSSDSEKLKLYTIY
                     SPAEHQDGVVRKTKADAEAKEEHFDGKTTESQ"
     gene            complement(11110..11325)
                     /locus_tag="P5056_01330"
     CDS             complement(11110..11325)
                     /locus_tag="P5056_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62394.1"
                     /translation="MIKFDPVINDSCTVVNETIIQVGGRRVISRSPIGYIKKGKFEYL
                     VPFGFTYDDMILIANKTKEVSGNTPHT"
     gene            11563..12129
                     /gene="yihA"
                     /locus_tag="P5056_01335"
     CDS             11563..12129
                     /gene="yihA"
                     /locus_tag="P5056_01335"
                     /inference="COORDINATES: protein motif:HMM:TIGR03598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HRY62395.1"
                     /translation="MKIESAIFRKGIRGDDRILNEDKPQIAFVGRSNVGKSSLLNCLL
                     NRKDLVKSGKMPGKTREINFFLINGKYYFVDLPGYGFAKLPIDVREQIAKMIQWYFFQ
                     KVFHRKAVLVLDAKVGPNAMDLEMYRILRDQKEEVIIVANKIDALNQKERNTQLKLMA
                     EEMPGVEIIPCSAKTKEGREKILEKIFS"
     gene            complement(12126..12575)
                     /locus_tag="P5056_01340"
     CDS             complement(12126..12575)
                     /locus_tag="P5056_01340"
                     /inference="COORDINATES: protein motif:HMM:NF023395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HRY62396.1"
                     /translation="MLYNDYLKTLNKCPFYDEIHFANQLVADNKTSALIISLAPYHKH
                     HLLIIPKRHLERIVEISDEEMSDIIELQRIGIKILYRLGYSNMSVLVREGENIGKSVK
                     HLHYHIIPNVMIGAENITGGDERSISTEEEILEFIEDMREAMEDLSD"
     gene            12655..13398
                     /locus_tag="P5056_01345"
     CDS             12655..13398
                     /locus_tag="P5056_01345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62397.1"
                     /translation="MNTSFLFLCLLTIICWAVAVFLHSKKDSITLTPLYAYVAILTVL
                     THNLSDLGFKLVLNDWVFIVSSFSMFTTLMFCILLLYLFEGPRAGRFALWIILFSSFF
                     YIGVVFFLGFITDTSSWILIDSARISYYFWSILAIIIDVIFLAIAWEVLSRATSLNLL
                     LRVFVIIFGVFALDTLIFTTGVFGTSEMYLSILQGNLLMRLALSAFGAPVMAYFLIAG
                     GFSEDKRDRPKSVLEILNFQSDLGEKKTF"
     assembly_gap    13519..13528
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(13752..>14076)
                     /locus_tag="P5056_01350"
     CDS             complement(13752..>14076)
                     /locus_tag="P5056_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62398.1"
                     /translation="NGRIKTEENPAITEHDTSVNLKPANDTVITAPSFSQSPGSNKEL
                     LGPHATTSRIKAVITRSINQNLQIAAIYEAFLPEFLKVSWLITILLIILFLLVRKRKK
                     DNKKE"
BASE COUNT         3793 a         2732 c         3240 g         4301 t
ORIGIN      
        1 aggaaagccg ccaaggccgc caaagacagt gttctcagaa agggcgtatt ggatggacta
       61 agcttgccgg gtaaacttgc tgattgtcag actaaaaacc ccgaagaggc tgaaatattc
      121 attgtagagg gagattccgc cggaggttct tctaaacagg gcagagacag aagaacacag
      181 gccgtgttgc ctctcaaggg aaaaattctt aacgttgaaa ggacaaggct tgataaagtg
      241 ctttcttcta acgagattaa ggcgcttgtt attgccatgg gcaccgctat cggagacata
      301 tttgaccaga caaagcttag atacggaaaa ataatcatcg ctacagatgc tgatgttgat
      361 ggtgctcaca tcaggacgct tttgctcacg ctcttttaca gatatttcca gccgcttgta
      421 tctgcgggaa atatttatat tgctcagcct cctctctata aaataaagaa aggaagggat
      481 gtctcttacg cttatagcga cgaagaaaaa gaacaaatcg ttaaatcgtt tggggcgggt
      541 gttgcggtag agcaggagag tgagggcggc tcagtaggcg aggagggggc ggaggaggag
      601 acgggagaga cgataaagaa atcaacaaaa gtttctatac agagatacaa aggtctcgga
      661 gaaatgaatc cggaggagct ttgggagacc acgatggacc cagagaggag aatactcaaa
      721 caagttacga tagattccgc cattgaagcc gataagacgt tcgatgttct tatgggttca
      781 gaggttttgc cgagaaaaat gtatattcag tcacacgcca agatggcaaa catcgacgcg
      841 tagaggatag agcaaaatgc aaaagtcaaa atgcaaaatg acatataaaa atgtaaaaat
      901 tttagatttt acattgtcat ttttattttt gattttttat atttccattt tagctactcc
      961 tctcatttcc tttgcagata ctttgagtaa cgtaggtatc ataaaaagcg gtatttggta
     1021 ttccaaagat cctttttatg ccggagataa gattagaata tatactcttg ttttcaacgg
     1081 cggggaatac gatcttttag gagacctgac cttcgacgat aacggcaagg taatctgcac
     1141 aggaaatttt tcggccgctt caggcaggac acaagaattg tggtgtgatt ggacggcaac
     1201 catcggcaca cataaaattt ccgcaaaaat aataaaccca aaagtttccc ccataggaga
     1261 atctcctcga acaataattt tggaaaataa tgtaagcggc gtttcagaga aaaaagtaac
     1321 ccttcctccc caaaaagaag aggagtctct tgtttctgtg cccaggatag catcttcatc
     1381 tgcgacaagc acaagcaccc ttatagaaaa gggattagat agtttcaagg atattgtttt
     1441 acaggttttg cccgaaaaaa taacaagtca ggatgttgta agccaaggag atgcggtcgc
     1501 atcagccact ccaaagacaa ttcgggaagg tgtcggcaag gtaatggaaa aaattggcgc
     1561 agagaaatta aaaacgcctg tgatgttcat cgtagacttc cttgtggtgg tctacaattt
     1621 cctaatgaga cagccggtcg tcctcatagc acttttgtcc tttattgttt ggaaatttgc
     1681 caaataccta tacggaaggc tagtatagct cttatgggct caaatttgaa ttttaagata
     1741 gaaaagaagc tccccggacg actcggaagg gtgggggttt tggagacccc acacggcaaa
     1801 atagagactc ccgcgtttat tgttgtgggc accaaggcta ccgttaaggc gctttctccg
     1861 gaagatgtgc agactctcgg cgcacaggcg gtgctttcaa atacatatca cttatatctc
     1921 gagccgggcg acgaactcgt gaagaacgca ggcggtctgc ataaatttat gaattggagt
     1981 gggccgactt tcactgattc cggaggattt caggtttttt cgcttggagt agcaatggaa
     2041 gaaaaggcga ataagttcgg agccaaaaaa caggaaacag tcaatgaaaa tatctgtaag
     2101 gaagagggga acacaaaact ttcaaaaata gacgaagatg gtgtgacttt tagatcgcat
     2161 atagacggca caagccacag atttactccg gaacgatcga tggaaattca gcacaatctc
     2221 ggcgcagata taatctttgc ttttgacgag tgcacatctc ctctggcttc acgcgaatac
     2281 cagatagagg cgatgaaaag aactcataga tgggccaaaa gatctctcga caggcacaat
     2341 gagttagggg ggccgcagtc gctttttggc atagttcagg gtggtaggtt tgaggactta
     2401 agaaaagaga gtgccaagga gatctcttcg ctgctgtttg atggatttgg aataggcggc
     2461 tcattcgaca aagaggatgt ttataaagcg ctggtttggg tgaacgagat attgccggaa
     2521 aataagccga gacacttcct gggaataggc gcggtagagg acctttttgg cggcattgaa
     2581 aatggcgtgg atacttttga ttgtgtggcg ccgacaagaa tggccaggaa cggcgcactt
     2641 ctcacaaaag aggggaggat gaatatcatg aacgctaaat ataaaacaga cttcactcct
     2701 atagagggcg actgtgaatg ctatacctgc aaaaattata ctagagcata tttggcgcat
     2761 ttgtttagga cagaagagct ctttgcatac aggctttcct caatacacaa tctttatttt
     2821 gtcgttaatt tggtaaaaaa aattcgccaa tcaatattgg acgacaaatt ctttgagttt
     2881 aaggagcagt ttttaaatag atataattcg ataaaaaatt aataagaaaa aagggcgacc
     2941 gtggcggtcg cccttactgc ttgctgttgt tgtttgtttc tacttcttcc ccttgccctt
     3001 gccgccgccc ttcttgccgt tcttcttccc gccgcccttc ttgccgtggt gcttcttgga
     3061 ctcgccgtcc tcgtcctcga cgcggatgcg cttggtgcgg ggagccttgg ggggcttctt
     3121 ccgcatcgtc ggcggcttct cgatcaccat ggcctcgagc ccggcgacga ccgccttctt
     3181 ctcggccttc ttgatctcgt cctcgcggtt caacgtgaag ttctcgacgt tgttcgagag
     3241 ggcctcctgg atcttggtgt cgacctcgcg ggcggcgtcg atgcgggcgt acgcgacctt
     3301 gtagttgagg aactcgacga tcttgtcgat ctgcttcagg ccggtcggcg tgcaggtgtg
     3361 gaaggtgatg gcgacgtccg cgaccttgat ggccgtcggc gtgtgccggg gcgggatgag
     3421 gacgtccttc tcgccgaact ggaaagtgcc gtcgtagttg cgggccaccg tctgcttctt
     3481 gcccttcgtc caggtgttct ggggcgggag gggctcgaag atgttgttga cgtggttcgc
     3541 gccgatgccg gtctggccga ggaacatgcc gttcttcatc cgcttggtgt ggcggccagt
     3601 cgactcgccg cccttcgtga aggtggcgaa ctcggtcttg tagccgttct ccgaccgcga
     3661 gatctcgtgg gcggtctggg tgaccgacgt ggtggtgagg ggcgtctcgt tctcgttcat
     3721 ggtgtctgaa cctccttagg ttctgggacg gtaagtggaa cgtgaatgtt tcgtacccct
     3781 agggctttaa acaaacaata tataaaggtg cttatttgac tttctcttat aattatacac
     3841 ctttcattat atttgtcaac cccagtagta aagcagagct tactacgggg ctacggccca
     3901 ttattttaag ccatattctt gtggaaacat tctaacttta taaccttcaa cttttaactc
     3961 attttagacc atctaccaaa tacgccttgc ggcagaatcc tgtccccgcc gtgttctttt
     4021 agcccaagct catctgtttc tatcttcccg cccttgtctt taaaagcggt ttttaagata
     4081 ttcgctataa ctgtcgggga catacccgtg gtataggagt ttattatgaa gaagaggggg
     4141 ttgtcgctaa gaagtcgtga cgttttatct acgagtggcc acaatttttc ttcaagcttc
     4201 cagacctcgc ccttggctcc tcgcccgtat gatggcggat ccataattat tccgtcatat
     4261 ttcactccgc gttttatttc tcggtcgaca aattttagtg catcgtcaat tatccatctg
     4321 acctctccgc ttttgttatt taactcgata ttttctttcg cccatgtgac aatcccgttc
     4381 gaggagtcta cgtggcatac ttttgcaccg gcattcgatg cggcgaccgt agccccgccg
     4441 gtatatgcaa agagattcag gacagatatc tctctatgtg ctccttttat tttttctttc
     4501 atccagtccc agttcgcgga ttgttccggg aagagacctg tgtgtttgaa aggcgtcggt
     4561 ttaatagaaa acttaagatc tccgtatttt atctcccatt tttttggcgt gccctctttg
     4621 aatttccatt cgccacctcc accctcgctt ctagtgtaaa taccgtcagc atttcgccat
     4681 tctttgtcgg acaatgattt cggccaaaaa gcttgcgggt cagggcgcga caaaaccaca
     4741 ctgccccagc gttctaattt ttccccgtct cctgaatcca ggagttcata atctttccag
     4801 ttgtttgcga ttaacatttt gcgggtccag tagggctcga acctacagtt ctggttttgg
     4861 agaccagtgg tttgccatta accgatggac ccaccttgtt tgcctataaa aattgtgatt
     4921 atattttaca taacactata tcatagttca atattagata caaaaaatcg ccttttaggg
     4981 gcgattttta aagatgtcga ttatttcttt atttccttgt gctttgtctt ctttttgcac
     5041 catttacaga acttcaaaag ctcaatcttt ctctcgacga gcttcttatt ctttctgctc
     5101 cagtaattaa tccttttgca ggatgtacag gccaatttaa ttaatgattt ctgtggcatt
     5161 gcttatattt aaactgattt ggtaaggata atacaaaatg gataaaaaag caagaggtgt
     5221 cccgcccttg gcggtttgcg cctcgctgtc ccgtaggccc tgttaaatag cgcttagcgc
     5281 taatttgccc gagacaaatt atatttaata gggtctttct gggacaagcc caacagtaaa
     5341 aaataaagcc ccacagttac gtggggttta ggggtctgac ttggtcattt ttttgccgtc
     5401 tttatggcaa atacttcttg tttcatcaaa taaaattggt agaataatga ggacctgaag
     5461 acacaagata tcaaatgcca aaaaagaaaa aagataaaat ccagaatggt gacgacggct
     5521 ctctcggtga tcttttcgag ctaccgaagg gcggcctgcc tatatcaccc gcgaaagatt
     5581 atttttcggt aggagaattt ctggacattc tcaatgagca cctgaagagc gagaaagttt
     5641 ccgtggcggg agaggtgacg gagtttcgcg ctcacgacaa atgggtgtcg ttctcgctta
     5701 aggacaaaga agataaatct atcttgaaat gttttctctc gatttggacc tatagaagcg
     5761 ccggcgtgct ccttgaagat ggcatggaag tgaaagtgag cggatatccg agaatatata
     5821 aactcgcggg aaccttcagt tttaatgtgg agacgataga gccactcggg gaagggtctc
     5881 ttaaaaaagc atatctgttg cttcttaaga tgcttgaaaa ggaggggctt tttgcgcgaa
     5941 aaagagcgct tccaagattt attaaaaaca tcggcgttat ttcttccaaa gacggagtag
     6001 ttatccacga ctttaggaaa aatcttaaac cgctcggctt tcagataaag ttttttaatt
     6061 cgcgtgttga aggagcggac gccgtgccgg gaataatcgg cggaataaaa tggctgaaca
     6121 aaaatatgcc ggatctggac gttatggtaa taatgcgcgg agggggaagc cttgagagca
     6181 tgcaagcttt taacaacgaa caggtttgta gagaaatttt ctcttccaag ataccggttt
     6241 tgtgcggaat aggacacgaa gtagacgtgc cgattgcatg tcttgtggcg gatgcttatg
     6301 tgtctacgcc taccgccacc gcttatacaa taaatgagag ctgggacgac ctcgtttcgg
     6361 gtctccctat gtttgagcgt aggataatca ctcagttcgg attttatttg aaggagtttt
     6421 cttccaagct tccgtatctt tggaaaaata tttacggccg cttcggtgac aatcttgccg
     6481 gtcttaaaaa agaggcgagt ggtatttcag gaaggatatt ttccgccttc aggagagtat
     6541 ttgttcagtt cgggattatg tcggacagaa taacgaagga tgcaataaga ataataggag
     6601 acaggctgac tcattacatc tcccaaatac aaaagtttga gaaaataatt gcggtcgcag
     6661 acccggagcg aaatctacgt ttgggatata gtattgtttt tgacggcagc ggtcgtgtaa
     6721 taaagaacgc gaaagatgtt aagaagggtg aggtgataaa aacaaaatta ggaaaaggag
     6781 aaatagaatc ggaggtgaaa tcaattacga attaacaatt acgaattatt aattaaatat
     6841 taaatcgatg gcgacaaaaa ataacgatat aggtgaatca ttaaaaaagc ttgaagcaat
     6901 cgcttcctgg tttgaaaaag agaatgagat agatgtcgag gaagggctca agaaggtccg
     6961 cgaaggagcc gctctcataa aagacctaaa gagcagactc gctgaagtaa cgaacgaatt
     7021 tgaggaaata aaaaaggagc tgacaaaaga cacagagtag attttgtgtc tcttgtcatc
     7081 cccgcgtagg cggggatcca tttttttatg atcccagatt aacctctgca aaatttctca
     7141 gccgttttta taattttctc tatttgattt ggtttaaacc atttcactct tttgtctttg
     7201 cgaaaccaag tcatttggcg tttggcatag tgctcgattt ctttatttaa ttccgagatc
     7261 atttcttctt tattgataag gccttgtagg tatcttgcca catacctata ttcaagcccc
     7321 atatctttca ttcttgcgaa agacaggcct tctttgtgaa gtttttttat ctccgcaatc
     7381 attccggctt ttatcctttt tagcagtctc aggtttattc tttctttcaa aattttgtct
     7441 cccgggtata atccaatctc caaaatttcg tattttgggg ctttgttttt tagcgtaggg
     7501 acttttccaa gcgcctgcgc tatctctata gcgcggataa ggcggaccgg gttgtgccta
     7561 tctatcttct cggcacgtcg tttgtccatt tttttaagaa tagcaaaaag ttcgtcagcg
     7621 tttttgcccg cgagttcttt gcgcagtttt ttattaggcg ggacatcggg aatctccaaa
     7681 tcatctatta tcgcgcgaat atagaagcct gttcctccgc agagtatcgg gactttgcct
     7741 ttttgtaata ttttttctac cgccttgcgg gccattttct gataatccgc tacggtgaaa
     7801 attttttttg gatttgcgac atcgagcagg tagtgaggga cccccgccat ttcttttttg
     7861 gtaatctttc ccgtgcctat atccatgccc ttgtagacct gcctcgaatc cgcagaaatt
     7921 atttccccgc cgatcttttt cgcaataaga acagccacat cgctctttcc ggaagaggtc
     7981 ggtccaacga cggcgataag tttatctttg gatttgtgtt tttctttcat ttataccaat
     8041 attatgaatt atgaaggaaa aatcaaacag caagacattc tggaacaaag aatataaaac
     8101 accgggaatt tttaatttgt ccaccgagcc cgcagaagac ctgctcaaat ttacaaggtg
     8161 gctagaaaga gaatacggta aaggattttt ggatcagaga agttttgtgc tcgacttggg
     8221 ttgcggtaac ggtagaaata ttatttacct tgcgcggacc ttcggttgca agggtgccgg
     8281 atatgatatt tctgatgaag cgatagagca ggcgaaaaag gctagcgcgg gattacatat
     8341 acaattcacc gcgcgaatga tagaggggaa gatagatctg gaagatgaat ctgttgatgt
     8401 tgtgctcgat atgatggcct cacactatct tcgcgagaaa gaccgagata tttatttgaa
     8461 agaaattacg agagttttga agccgggcgg ttggctcctt ctgaaatctt ttcttaaaga
     8521 agaagacagg cacacagaaa ggcttctaag ggataatccg gcaggggagg atgatgctta
     8581 tattcatcct gagcacggca catacgagca tgtttgggcg gaagaaaaaa tgcgcgcgtt
     8641 ttatggcgag gcttttgata tagataaagt agagcgctcg ggtaaacacc ttatgaaggg
     8701 caaggccttc aaaagacgcc attttgtggc ctatttgcgt aaaagatact aaatcgcata
     8761 aaataaggcg tttttgaata tctattgact ttttagtctc attgtgctat acttgtaaaa
     8821 gaaatagaga ataagttctt taattcggtt tcgcacaaaa ggcatgcctg cgggcatcca
     8881 cggatgaagt ttcggcttca aaatcgaaag gtggacatta tgctttgccc tctctgcaac
     8941 gcggaaatgc ataagaaccc gaaggtcagc ccccgcaaca aacacctctc tctgaagtgc
     9001 ccgagatgct ccttcgttat cttctacaag ggggtcgaga gcatgaagaa cttggacccg
     9061 gaaccggaac cgaaggagaa gaagtagctc gacgacagtt cgagagccca aaaggccagg
     9121 attaattccc ggccttttat ttatgcggat tattttcttc cttgtttatt gtttatctaa
     9181 tataatataa ggatgataat cacatatata attttattgg tgttgttgtt ttatgttctt
     9241 ggcgcttcag ccgatatggt cgttcgccgt ataaaacaga ttgcagacaa gtttaaaata
     9301 agcattttgt cgatggggat ggttttggga gcgctgacaa cccttcctga attttttgtg
     9361 ggtgtgaact cgctggtgct ggacgtgccg aatctttatt tcgggacgtt gatgggcggt
     9421 atcattgtcg ttttttgctt gatactcggc tttagtttag ttttgaacag aaaagttaaa
     9481 acagacggaa agtattcaag cattttaccg gtttctcttg cgctcctgct cccgttattt
     9541 atggggctta agggcactct gaattatttg gatggtataa ttttcctggg tatttatttg
     9601 atatttctct ataaaaacac gatagagcat aatcacataa acgtttctga tataacaaag
     9661 aacgaagtcg tcctcgccaa gaattttttc tggattatta cgggtgtgat tctcgtagta
     9721 ttctcctcaa atcttattgt ggttatcgcg acacgtatcc ttgcggctta taacgtttcc
     9781 gctcttatga tcggcttaat tgtgttttct ttgggaacga atttgccgga gcttataatt
     9841 gccataagat cttggacacg gcacgtgccg gaattgtctc ttaatcatat tatcggttcg
     9901 gcattagcta atattttggt gctcggcttt atgctattgg ttaggccgct atcttttgat
     9961 ataggcgcct cctataagtt tttattaaca gtcatgtgta ttgttttgtt tactctcacc
    10021 tctttttaca agagcgatgg agagctcaca aggaaggagg ggtataaatt gttggtgata
    10081 tatttggttt ttattgtcgg acaaattttg ttttcttaaa aattaattaa atagtatgca
    10141 agcatataat tggtatagca cattaattaa accatcattt gccccgccag cgtgggtgtt
    10201 tggacccgtt tggaccgttt tatatatact catcgcaatc tcatttagct atgtttttta
    10261 tctttatata aaaggcagga tatcgttttg gattttattg ccgtttatat taaatttagt
    10321 ttttaatttt gcttttactc ccgtccagtt tggcctacag aataatttac tggcttctat
    10381 tgatgttgtg gcggttatcg ggacgcttct ctatgcgctg tatgctattt atccgtatgc
    10441 cgcgtgggtt gtgtatgcaa atattccata tttattatgg ggagcgtttg cgacggtttt
    10501 acagcttact attacgtatt tgaatatgta gaaattaaaa gtttaaattt ataaaaatga
    10561 agggatatgt atctaatata gaaaaagagt ctttggaaaa tgataatttc agaaaggttc
    10621 tttacacggc taaatatagt cagcttgtgc ttatgtcatt actcccaaaa gaagaaatag
    10681 gggaggaggt tcatagcgat gtggatcaat tttttaggat tgagaaagga accggtgtgg
    10741 tgattataga cggtgttacc cataatattt ctgatggttt tgcgataata gtgccttcag
    10801 gagccaaaca taatgttata aatagttctg attctgaaaa gctaaaactt tatacaatat
    10861 attctccggc cgaacatcag gatggggtag ttagaaagac caaggctgac gctgaagcaa
    10921 aagaagaaca ttttgacggc aaaacaacag aatcccagta ggaaagttca aattacgtat
    10981 tgcgattatt aaaataaata tctctcggaa ttcgccttcg actcctcttc ttttaatttt
    11041 gaccttttta ggtaaacttc ctgcgggata aataaaaatt aaaaccccgc gagcttcgcg
    11101 gggtttttgt tacgtgtggg gcgtgttgcc ggatacttcc ttcgtcttgt tggcgataag
    11161 gatcatgtcg tcgtaggtga acccaaacgg gacaagatac tcgaattttc ctttcttgat
    11221 gtatccgatg ggggaccttg aaatgacccg tcttccgccc acctgtatga tggtttcgtt
    11281 cacgacggta cagctgtcgt tgatgaccgg gtcaaatttt atcattgggt cctcctttgc
    11341 cttcggccct tgcgttataa attgtgcttt tctctgccgc tcactatacg cttattaaaa
    11401 atattgtcaa gtttgttttg aggcatacaa ccgaataata aagataaaaa atagacagaa
    11461 cctccggggg aatatttatt ttgcgaaaaa gagaggtgca aaagagaata cggcgatgaa
    11521 gaataatgac aaggcggtag atttcgtata acattcatta ttatgaaaat agaaagtgct
    11581 atctttagaa agggtatcag gggcgacgac cggattttga atgaagataa gccccagatc
    11641 gcttttgtcg ggcgttcaaa tgtcggcaag tctagccttt tgaactgttt gctcaaccgg
    11701 aaagatctcg ttaagtcggg aaaaatgccc gggaaaacaa gagagataaa ctttttctta
    11761 atcaacggta agtattattt tgttgatctg ccggggtatg gttttgcaaa acttcccata
    11821 gatgtcagag agcaaatcgc aaagatgatt cagtggtatt tttttcaaaa agtttttcat
    11881 aggaaagccg ttcttgttct ggatgccaag gtgggaccaa atgcgatgga ccttgagatg
    11941 tatagaatct taagagatca aaaagaagag gtaattatcg tcgcaaataa gatagacgcg
    12001 cttaatcaaa aagaaagaaa cacgcaactt aaactaatgg ccgaggaaat gcccggcgta
    12061 gaaataattc catgttctgc aaaaacgaaa gaggggaggg aaaagatact cgagaagatc
    12121 tttagttaat ccgatagatc ttccattgcc tctcgcatat cttctataaa ttccagtatc
    12181 tcttcttccg tagaaataga tctttcgtct ccgccggtga tgttctccgc tcctatcatt
    12241 acgttcggta tgatgtggta gtgaagatgt ttaacacttt tgccgatgtt ttctccttct
    12301 cgcacgagaa cagacatgtt tgagtaaccg agtctgtaaa gtatttttat ccctattcgc
    12361 tgaagttcta ttatgtccga catttcttcg tcgcttatct ccacgattct ttcgaggtgt
    12421 cttttgggga ttataagtaa gtggtgctta tggtatggag ccaaagaaat tataagagcg
    12481 gaagttttgt tgtcggccac taattgattt gcgaagtgaa tttcatcgta aaaagggcac
    12541 ttatttaatg ttttaagata atcattataa agcatatcct aattttatcc gaaaaaacaa
    12601 aacaataaaa attctctcat tgttgataag gcgttatggt gctattattc tattatgaat
    12661 acatcttttt tgtttttgtg tttattaaca attatttgtt gggccgtggc ggtattcctt
    12721 catagtaaaa aggacagcat cacactgact cctttatatg cttatgtggc aattttgaca
    12781 gtccttactc ataatctaag cgatttaggt ttcaagctgg ttttgaatga ctgggttttt
    12841 atagtgtcct ctttctctat gtttaccact cttatgtttt gtattctgct cctgtatctt
    12901 ttcgagggtc ctcgggccgg aagatttgct ttatggataa tattgttttc ctccttcttt
    12961 tatataggag ttgtattctt ccttggtttt atcacggaca caagctcttg gatattaata
    13021 gactcggccc gtatttctta ctatttttgg tctattttgg cgataataat agatgtaata
    13081 tttttggcta ttgcatggga ggttctgtct agggccacat cactaaattt gttactgagg
    13141 gtttttgtta ttatttttgg ggtttttgcg ttggatacac ttattttcac aaccggagta
    13201 ttcggcacct cggaaatgta tttatctatt ttgcagggca atttgctaat gcgtctggcg
    13261 ctatctgctt ttggagctcc ggtgatggcg tatttcttga tcgcaggagg atttagtgag
    13321 gataaaagag acaggccaaa aagtgtcctg gaaatattaa atttccaatc agatctgggt
    13381 gaaaagaaaa cattttaaat aaacattgaa aaactcccct aaaaggggag ttgttgtttg
    13441 ttctcagtcg ctggagtcaa caaggtagaa atatttgcaa atactgcact catacttgcg
    13501 gaggcacctt ctgatattnn nnnnnnnngg gatatgcttc agttttttac attttgctat
    13561 ttcgcgcgga gtcttttctc cccacgaatt gatataaccg agattttttg gcgccccttt
    13621 tttcttcatt gttgatcctt tccctggaat caaaatttta agttacaaat ttagataaca
    13681 cacgtaatgt tatttatcta tatagaaaat atagtatatt atagctcttg tcaagttttg
    13741 cgttacagga tttactcttt tttgttatcc ttttttcttt tacgcacaag tagaaataga
    13801 ataatcaata gtattgttat aagccacgac acttttaaaa attccggcag gaacgcttcg
    13861 tatatcgcgg ctatttgaag attctgattg atgctccgcg tgataaccgc ttttatcctg
    13921 cttgttgtcg cgtgggggcc aagtagctct ttgtttgacc cgggggattg tgaaaacgaa
    13981 ggggctgtga tgaccgtgtc gtttgccggt tttaaattta cggatgtgtc atgttcggtt
    14041 atggccggat tctcttccgt ttttattctt ccgttc
//
LOCUS       DAOTEX010000013        14048 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3118
            NODE_3002_length_14048_cov_4.489030, whole genome shotgun sequence.
ACCESSION   DAOTEX010000013 DAOTEX010000000
VERSION     DAOTEX010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868142
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 14048)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14048)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 883
            CDSs (total)                      :: 833
            Genes (coding)                    :: 829
            CDSs (with protein)               :: 829
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14048
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3002_length_14048_cov_4.489030"
                     /isolate="AMR_MDS_3118"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..854)
                     /locus_tag="P5056_01355"
     CDS             complement(<1..854)
                     /locus_tag="P5056_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62399.1"
                     /translation="MMTTKKLITSDDPKGLDATAKWRAVYNKQQLTDGADGSAQRLNE
                     SREFWKELGELIQKHSATNQYAGEVVSSNYTYPKEYKGPKPIAEQVNTIATMFGLDPT
                     QALEFAKNLPTLPEGSEGWFAIPKVSVVATKQFPAITDKAEQYCEAVKLVHTKLADSR
                     SFYNYRNGEIVPSKLRQHARTVDFLEHLEAEQQGDILIIAVQYGMRHRGKSVRRARET
                     FASNEFGLGAFAIGCMALVHPERYVRWEELDTDCAGDEFAPDADGDFSGSPLFRFDDG
                     KLKFDTNAV"
     gene            complement(955..2448)
                     /locus_tag="P5056_01360"
     CDS             complement(955..2448)
                     /locus_tag="P5056_01360"
                     /inference="COORDINATES: protein motif:HMM:NF036421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62400.1"
                     /translation="MTKIIPNALVNTMLKDRSVRTSITKDSFLYFFHFYYAHYVKYET
                     ADFQKEIINNLEKSTVENLYIVAFRGSGKSTMVTTAYPIWAILGKQQKKFCIIFCQTR
                     AQSKQHMMNIRTELEGNDVLKKDLGPFQEESDEWGSFSLVFKKHGARITVASSEQSIR
                     GIRHNEHRPDLIICDDVEDVQSTKTREGRDKTYNWLRGEVIPAGDKNTRLIIVGNLLH
                     EDSLLMRIKDEISKGKAKGTFSEYPLLDRNNKCLWSGKYRSEKEIEDEKLKVASDVSW
                     QREYLLRIIPDDDQVIYPEWINYYDELPGEKHRGYRGTYAGVDLAISASETADDTAVV
                     FAHIYSRREKMRIYILPNPIAKKLNFPAQVDLMKDIRSTMLIKSTDELFVESVAYQEA
                     LPQMLEHQGVKATAIKPKGDKRTRLALTSTAIKSAKIKFPRQGCERLIEQLVGFGVEK
                     HDDLADAFSLLINSTMDKHSKESTWLISFLGGSKDDTLYFRDYIDME"
     gene            complement(2441..2857)
                     /locus_tag="P5056_01365"
     CDS             complement(2441..2857)
                     /locus_tag="P5056_01365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62401.1"
                     /translation="MKKNKFQDQFLDELRKVPIVQVACEKTGLSRNSVYRWRKDDKEF
                     LKKMDSALSDGVALVNDMSESQLLTLIKEKNYPAISFWLRHRNDNYKNKLEITTKEDN
                     EELTPSQAKIVKQALKLAKITKSKSIKNINKSKSHD"
     gene            complement(2844..4118)
                     /locus_tag="P5056_01370"
     CDS             complement(2844..4118)
                     /locus_tag="P5056_01370"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA modification methylase"
                     /protein_id="HRY62402.1"
                     /translation="MKKLSWTTGVREVKDLLPWQENPRKISEQALDKLKEKITQNGFH
                     SVIVIDTDNTILSGNQRKTALMELGINTVDVMIPSRKLSDAERRRIGIESNINDGEWD
                     FEKLKSFDLELLQFAGFDEKELVKFWDDEKDTKDDKFDIDKELKKIKTPQTKLGDLII
                     MGNHKLLCSSSTNITAVKKLFGDDRATAIYSDPPFNIGLSYDKGVGNKSNYGGSFNDN
                     QSPEAYKEFIRQVLKSSLAISTKDTHVAFWCDEAWVWVFQTLYMELGVKNRRLNIWIK
                     NNSSPIPTVAFNKCAEYCVYGTKGSPYLSDLVKNLNEIQNKDCTTGNQLLDDISNIWA
                     TKRLPSNQMEHPTSKNPELHHKFIMRCTKPGDIIFDAFSGSASTMICAEQLDRKVYSL
                     EIEPVFCDLAIRRYEKLTGRKAQVIKKFYEKE"
     gene            complement(4279..4686)
                     /locus_tag="P5056_01375"
     CDS             complement(4279..4686)
                     /locus_tag="P5056_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62403.1"
                     /translation="MQLNRVTQLPKKGEKVIVLELTDIQLHSNARPTQSVGGAIRRSI
                     ENNKPGRIFSSLEKKQESVTDQNSIEFSMPFVMNDPVLKQTIHDYQKQGYRVMIQISK
                     SGLPIHLGKDAVDFMNSTRGKRILRKIEKDKDY"
     gene            4777..5418
                     /locus_tag="P5056_01380"
     CDS             4777..5418
                     /locus_tag="P5056_01380"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24 family peptidase"
                     /protein_id="HRY62404.1"
                     /translation="MHNKQKQLLALIDTDPNIGSYSLRTIADKIGVPDKPQIVKYHLQ
                     QLEKAGMIQMNLEQGVIKPVKRGLVKSTVNTVFSLPVVGSANCGPATIFAEQNIQQYL
                     KVSSSMLPYNKKDLYSVIADGNSMNLAKVDGKNTIESGDFVLVDSGYKNYKNGDIVVA
                     VIDGMATIKRYKDDKANERIVLEADSTDKYLPIFIHEGDDFQLSGKVVGIIKS"
     gene            5444..5671
                     /locus_tag="P5056_01385"
     CDS             5444..5671
                     /locus_tag="P5056_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019383841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HRY62405.1"
                     /translation="MAKNIHTVFNSDRHMWENKAEKSSTPLSSHHTKENALNRAENIA
                     EKAKVEHFIHGKDGKIQERNSYGNDPFPPRG"
     gene            5675..6592
                     /locus_tag="P5056_01390"
     CDS             5675..6592
                     /locus_tag="P5056_01390"
                     /inference="COORDINATES: protein motif:HMM:NF014021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HRY62406.1"
                     /translation="MSIPLSQLETWSNQGATVSSKTTHESIRCALIHDLSPLKDRISS
                     GSVKIYLQGSYKNDTNIRADSDVDVVVELTTTFGHNAHELPYEQKNAHDQAYSSAVYD
                     WQDLRRDVISALENYYDVASVDTSGNKSIKILPSSGRLKADVVPVINYRKYDYFYGTD
                     THSSEQGVKFYHRTTNRAIVNYPEHHFKNGAQKHQNTQNQFKQVVRILKNARNYLVEK
                     NLLDKEKAPSYFLQSLIYNVPNSCFSNDLSASTRSVLNHLYTNPIDNFICQNGLTPLF
                     GTSEEQWNTDDAVATIVALVDLWDNWYER"
     gene            6601..7191
                     /locus_tag="P5056_01395"
     CDS             6601..7191
                     /locus_tag="P5056_01395"
                     /inference="COORDINATES: protein motif:HMM:NF037740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62407.1"
                     /translation="MHPYSINSEEHKRTSFCIAVFAIMLAWGLYLFSKNVMSLPWWIE
                     APSVIGFYGILYKWFDIKLWRNKHIRKLLGIRTPIVDGSWSGEINSASVYSKGPIPTK
                     KFCIKQTWTHVSIYLETETSESHSFEASMNVDQFDTARIHYQYMNVPKDSAPETMHIH
                     YGSATAKLTDENSLETEYYSGRDRNNTGSFKVIKLQ"
     gene            7602..8180
                     /locus_tag="P5056_01400"
     CDS             7602..8180
                     /locus_tag="P5056_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62408.1"
                     /translation="MNPKYDQNEVMLDLQFVAKNGGREKIESELAKLADYEKRLASAN
                     LLKYEVDLDVTPKCPGNLKIAEEKHQIASRVKGKIEVGSADDVKKLVNLHLDPGQMNG
                     KCIGSHKLKKKLEGQPVYGAVLLDFLLEHPEFIPDEWKTKGLIYFWGTTFRSSGGGLC
                     VRRLDWGGGRWYSDFYWLDYVWYGHGPAALRA"
     gene            8384..8950
                     /locus_tag="P5056_01405"
     CDS             8384..8950
                     /locus_tag="P5056_01405"
                     /inference="COORDINATES: protein motif:HMM:NF012307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HRY62409.1"
                     /translation="MCERCKKFFASIDHETLLQISARHIDDRDALWLMGRVISSFHST
                     REGVGLPLGNLTSQLFVNIYMNGFDQFVKHKLKVKYYVRYADDFVILSEDRESLNNVL
                     IVMGDFLKRRLRLNLHPDKVFIKTLASGVDFLGWVNFFDHRVLRAGTKRRMVRRIDEK
                     ASRETINSYLGMMKYGNGYKSTKKIFCN"
     gene            8975..9376
                     /gene="ruvX"
                     /locus_tag="P5056_01410"
     CDS             8975..9376
                     /gene="ruvX"
                     /locus_tag="P5056_01410"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HRY62410.1"
                     /translation="MYISLMRLLGIDYGTKRVGVALSDEAGLFAYPYSVLKNSKNLSK
                     EIIEICKKEKVGKIILGESLDYKGKCNNIVCETDILKNILEKETGLSVIYENEVLTSA
                     EAERIQGKTEMLDAAAAAIILRTYIDRQKNN"
     gene            9437..10528
                     /gene="pilM"
                     /locus_tag="P5056_01415"
     CDS             9437..10528
                     /gene="pilM"
                     /locus_tag="P5056_01415"
                     /inference="COORDINATES: protein motif:HMM:NF022554.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein PilM"
                     /protein_id="HRY62411.1"
                     /translation="MAKKSFFDLFPVPEYLSYPAVGLDLCGYAIKFIEFSRNSGKLKI
                     EKYARRPISETLPSNSPQEQVDEAIINTLIGIKEEFGIRAAHISLPEEHSYFLKLKLP
                     KTEAKEIRSSIELQLEEHVPYSPEEITFDYEVINTLENGEVEIIVSVLPSSVIDRYVN
                     IINRAGITPISFETEAASMMRAVLPQDFTKSALVVDIGKTSSTLSIVSKGIVWFSYTM
                     KTGGALFTKVVSEALSVSEEEAEKIKFNKGLIRSPENQEVLFALAPAISSLRDEIIKH
                     YWYWGAHKEISNQGEIEKVYLCGSQASINGLAEYLSVSLNLPVFLANPWENVFSFEEN
                     IPELPLASSLEYVTAIGLALKGVQDNNKK"
     gene            10530..11165
                     /locus_tag="P5056_01420"
     CDS             10530..11165
                     /locus_tag="P5056_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62412.1"
                     /translation="MNLLPLNEKKQARFEYLLRFWTVAGMATIVLLVAAATPLIISYF
                     TISYNVDFIKDVAENLSKSEGFKKTDTVAKIIKDVNRKSAILNDPLSTTARQDLLSSF
                     SFVFKTADKMRINQKGIVKIKQIIYEQVVKKGSARTGSVASTTASATSESGIHKINIK
                     GFSATRDGFLSFLKELESNKNFVMIDSPVSNLVNSENLDFSLTITLKDKAI"
     gene            11162..11764
                     /locus_tag="P5056_01425"
     CDS             11162..11764
                     /locus_tag="P5056_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62413.1"
                     /translation="MTDIKQKLKKKLYIVIVALAVTVIFYFYLFSVVREKQNTGLNSA
                     ISDLGRLESKQSNFSLNSRILDETTAERARLNSFILDKKTVADFIQQIEDLGKHANVE
                     ITKSLSVEKNPQKPKENILKFNVRAKGTLKDVYYFTLLTENLPYKIRVKKMAISTSDA
                     DTSSFSSGVMGVLEGKKKTAAVPSVWVSDINFEVISYINE"
     gene            11757..12092
                     /locus_tag="P5056_01430"
     CDS             11757..12092
                     /locus_tag="P5056_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62414.1"
                     /translation="MNKLFAKLNIFSPLRKFFDKDPEKVEPSPRFGWVLILILFLMFS
                     LAFMVLYYFMFLPQSDEALSGKPAFDPELSIENISKPKLQKTLEPWIAREKQFNDYLN
                     NRPSFDFLK"
     gene            12231..12302
                     /locus_tag="P5056_01435"
     tRNA            12231..12302
                     /locus_tag="P5056_01435"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12264..12266,aa:Arg,seq:tct)
     gene            12696..13778
                     /locus_tag="P5056_01440"
     CDS             12696..13778
                     /locus_tag="P5056_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62415.1"
                     /translation="MENMEQQNQKSREWRIVGKASLGKKNEFKEKMNKKLFAQTEAME
                     KELKQEQKDEVKAAEIEKTAEQIRLIELANEDTNKLMERLGFEPIDISPDNVYVIKSE
                     SFSKLKGPTVNAFANYNNQAIVINGEKPRLVQGGGAIFCTLTFHEMLHLKSHISFEAQ
                     SDKRKPELFRAGVTVYSSVKSDKEGMMHGHFRGLNEAIVALAEQDELKNILGTDEFKE
                     EGSWQDSVLAKDVKEDIPSIKDKDAIFVLRKKIEGQVPKTLFVSETLSYTKHKETLKY
                     LMQEILWDNQDNFISEDEVFDVFLRAHFSGGLLEIAKLVERSFGEGSFRQLGEMTDSA
                     SSAVSTLELFKKLRGKILREREKGQK"
     gene            complement(13976..14048)
                     /locus_tag="P5056_01445"
     tRNA            complement(13976..14048)
                     /locus_tag="P5056_01445"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14013..14015),aa:Val,seq:tac)
BASE COUNT         4060 a         2736 c         3061 g         4191 t
ORIGIN      
        1 acagcgttgg tgtcgaactt gagcttgccg tcatcgaagc ggaagagcgg agaccccgag
       61 aagtcgccat ccgcatccgg agcgaactca tcacctgcac agtcggtatc caattcttcc
      121 caacgaacgt agcgttcggg gtgaaccaga gccatacagc cgatagcgaa tgcacctaag
      181 ccgaattcgt tgcttgcgaa ggtttccctt gctctgcgaa cggacttgcc acggtgacgc
      241 ataccgtact gtactgcgat aatcaggatg tcgccttgtt gttcggcttc caggtgttcc
      301 aggaagtcaa cggtgcgtgc atgctgacgc agtttgcttg gtacgatttc accgttgcgg
      361 tagttataga aactacggct gtcggctaat ttcgtgtgta ccaatttcac tgcttcgcaa
      421 tactgctcgg ctttgtcggt gattgcagga aactgctttg tggcaaccac ggatactttc
      481 gggatagcga accatccttc agaaccttca ggaagcgtag gcaggttttt ggcaaactcc
      541 aatgcctgtg tcggatcaag tccgaacatg gtggcaatgg tgttcacctg ttctgcaatc
      601 ggttttggac ctttgtactc tttggggtag gtgtagtttg aagacaccac ctcacccgcg
      661 tactggtttg tagccgaatg cttttgaatc agctcgccaa gctctttcca gaactcacgg
      721 ctttcgttaa ggcgttgtgc ggaaccatcg gctccgtctg tcagttgttg tttgttgtac
      781 acagcacgcc attttgctgt ggcatcaaga cccttcgggt catctgacgt gatcaacttt
      841 tttgtcgtca tcatcttttt ccttgattta aaagaacact aaggaccgct tagtcggttg
      901 ctagatcatc tagctatgag ctactatagc atatttagtc tatttagtca atatctattc
      961 catatctatg taatccctaa aataaagcgt atcgtcctta cttccaccaa ggaatgaaat
     1021 gagccatgta gattctttcg agtgcttgtc catcgttgaa tttataagca atgagaaagc
     1081 gtcagcaagg tcatcgtgct tttcaacacc aaatcctaca agttgttcga tgagtcgttc
     1141 gcatccttgt cttggaaatt taatctttgc tgacttaatt gcagttgatg ttagtgctag
     1201 acgtgttcgt ttatctccct tgggcttgat cgctgttgcc ttaacacctt gatgttcaag
     1261 catttgtggt agtgcttcct gatacgctac gctttccaca aacagttcat cggtactttt
     1321 tatgagcatg gtgctacgaa tatctttcat gagatctacc tgtgcaggaa agttaagttt
     1381 cttcgcaata gggtttggca atatgtatat tcgcattttc tcacggcgtg aatatatatg
     1441 agcaaataca acagcagtat catcggcagt ttctgaagct gaaatagcaa gatcgacacc
     1501 tgcataagtg cctcgatacc ctcggtgttt ttcgcctggc aactcgtcat aataattgat
     1561 ccattcagga taaataacct gatcatcatc aggaataata cgcaatagat attctcgttg
     1621 ccacgataca tcactcgcaa cttttaactt ttcgtcttca atctctttct cgcttctata
     1681 ctttccagac catagacact tattgtttct atcaagtaaa ggatactcgc taaaagttcc
     1741 tttggcttta ccttttgaaa tctcgtcttt tatacgcatt aagagcgaat cttcatgcaa
     1801 gaggttacca acaatgatca gtctcgtatt tttatctcct gcagggatta cttcaccacg
     1861 aagccagtta tatgttttat ctcgaccctc acgagttttt gtagattgca catcttcaac
     1921 gtcatcacag attatgagat cgggacggtg ttcattgtgg cggattccac gaatactttg
     1981 ttcagatgat gcgacagtga tccttgctcc gtgcttctta aatacgagag agaatgaacc
     2041 ccattcatcg ctttcttcct gaaatggtcc aagatctttt ttcaaaacat cgttaccttc
     2101 aagctcggta cggatattca tcatgtgttg ttttgactgt gctcgtgttt ggcaaaagat
     2161 gatacaaaat ttcttttgct gttttccaag gattgcccag atcgggtatg cggtcgttac
     2221 catggttgat ttacctgatc cgcgaaatgc aacgatataa agattctcaa cagtactttt
     2281 ttcaagattg ttgatgatct ctttttgaaa gtctgctgtt tcatatttca cataatgggc
     2341 atagtagaag tggaagaaat acagaaaact gtcttttgta attgatgttc gcaccgaacg
     2401 atcttttaac atggtattta ccagtgcgtt tggaatgatt ttagtcatgg cttttagatt
     2461 tattaatgtt tttaattgat ttcgacttgg taatctttgc gagctttaat gcttgtttta
     2521 cgatttttgc ttgcgaaggt gttaactcct cgttatcctc tttggtggtg atttcgagtt
     2581 tgttcttgta gttatcgttt ctatgacgaa gccaaaagct gatcgcaggg tagtttttct
     2641 ccttgataag cgtgagcaac tgactttcac tcatgtcgtt taccaatgca acaccatcag
     2701 acagtgcact gtccattttc ttgagaaatt ctttatcatc ctttcgccaa cgatagacac
     2761 tattgcgaga tagtcctgtc ttttcacaag caacctgaac gatcggcact tttcgcagtt
     2821 catcaagaaa ctggtcttga aatttattct ttttcataga atttcttgat tacttgtgct
     2881 ttgcgaccag ttaatttttc gtatctgcga attgctaaat cacaaaagac gggttcaatc
     2941 tcaagcgagt agactttgcg atccaactgc tccgcacaga tcatcgtgct tgccgaaccc
     3001 gaaaaggcat cgaagattat gtctccgggc tttgtgcatc gcatgatgaa tttatggtga
     3061 agttcagggt tcttgcttgt aggatgttcc atctggtttg acggtagtcg tttagttgcc
     3121 caaatgtttg agatgtcatc aagtagttga ttgcctgtgg tgcaatcctt gttttgtatc
     3181 tcattaagat tttttacgag atccgacaaa tacggtgatc cttttgttcc atacacgcaa
     3241 tactccgcac acttgttaaa tgcgacagta ggtataggtg aggagttgtt cttgatccaa
     3301 atgttcaatc tgcgattttt tacacctagc tccatgtata aggtttgaaa aacccagacc
     3361 cacgcttcat cacaccaaaa tgcgacatgt gtgtctttgg tagatattgc aagtgatgat
     3421 tttagaactt ggcgaataaa ttccttgtat gcttctggcg attgattgtc attgaatgaa
     3481 cccccgtagt ttgatttatt gcccacgcct ttgtcatatg aaagcccgat attgaaaggt
     3541 gggtcactgt agatagcagt agctctatca tcaccgaata gttttttaac cgcagtgata
     3601 ttagtggagc ttgagcaaag caacttatga ttgcccatga taataagatc acccaacttt
     3661 gtttgaggtg ttttaatttt cttaagttcc ttgtctatat caaacttgtc gtctttagtg
     3721 tctttttcat cgtcccaaaa cttcactaat tctttttcat cgaatcctgc aaattggagc
     3781 agttccagat caaaggattt caatttctca aaatcccatt cgccgtcatt gatattgctt
     3841 tcgataccga tccttcgtcg ttctgcatca cttagtttac gactaggaat catcacatct
     3901 acggtattaa taccaagttc catgagtgca gtctttcgtt gattgcctga caaaattgta
     3961 ttgtcagtat ctattacgat tacactatgg aagccgtttt gcgtgatctt ttcttttaat
     4021 ttatctaacg cttgttctga gatttttctt gggttttctt gccaagggag taggtctttg
     4081 acctctcgaa ctcctgtagt ccatgaaagt tttttcatag cattttatta attaatcttt
     4141 aataaaatgt atggatatag aaaaagccta ttccacacac gaaataagta gaggtccgaa
     4201 aaacctcaag ttacttcttg cgcggaatag gcatggcttg tggcgctaca ctactcgctt
     4261 ttacgacgcg tatttatttt aataatcctt atctttctct atcttcctca aaattcgctt
     4321 tcctcgtgta ctattcataa aatcaacagc gtccttgccg agatgaatgg gtaatcctga
     4381 ttttgagatc tggatcatca cacgataacc ttgcttttga tagtcgtgga ttgtttgctt
     4441 caaaacagga tcattcatta cgaacggcat actgaactct attgaatttt gatcagtaac
     4501 tgattcttgc ttcttctcaa gagaagaaaa gatacgacca ggcttattgt tttctatgga
     4561 gcgtcgtatt gcaccaccaa ccgattgggt aggtcttgca ttagagtgca attgaatatc
     4621 ggtaagttca agaacgatga ctttttcacc tttctttgga agctgtgtta cccgatttaa
     4681 ttgcaaactt tgctccatag ggaaagtata tcagattaac gtcaaaagtg tcaaatttat
     4741 gttgttgcaa gttgaaaagt atgctataat gggtgtatgc acaacaaaca gaaacagctt
     4801 ttagctctca tagacactga tccaaacatt gggtcataca gccttagaac aatagccgat
     4861 aaaattggcg tgcctgacaa accgcaaata gtgaaatatc acttacaaca gctcgaaaag
     4921 gctggaatga ttcagatgaa ccttgaacaa ggggttataa agcccgtgaa gcgaggtctt
     4981 gtgaaatcaa ccgttaatac agtgttctca ctccctgttg ttggatctgc aaactgcggt
     5041 cctgcaacta tatttgccga acaaaacatt cagcagtatc tcaaagtttc atcttctatg
     5101 ttgccataca acaagaaaga tctctactca gtcattgctg acggaaatag tatgaatctc
     5161 gcaaaagtag atggtaagaa tactattgaa agcggagatt ttgttttggt agatagtggg
     5221 tataaaaact acaagaatgg agatatcgtt gtcgctgtta tagatggcat ggcaactatc
     5281 aagcgataca aagatgataa agcgaacgag cgcatcgtac ttgaagcaga ttcaacagat
     5341 aagtatctcc cgatatttat ccatgagggt gatgatttcc aattaagtgg aaaagttgtc
     5401 ggaattatta aaagttaatt attaaaagtt aattattaaa aatatggcaa aaaatataca
     5461 tacagttttc aattcagatc gtcacatgtg ggaaaataaa gcagaaaagt cttctactcc
     5521 actttcgtca catcatacaa aagaaaatgc tctaaataga gcagaaaata ttgctgaaaa
     5581 agcaaaggtt gaacacttta ttcatggtaa agacggaaag attcaagagc gtaatagcta
     5641 cggcaatgat ccgttcccac ctcggggcta aaatatgtct atccctcttt ctcaattaga
     5701 aacatggtca aatcaaggtg cgactgtgtc atcaaagaca actcacgaat ccatacgttg
     5761 tgctcttatt cacgatctct cacctctgaa agatcgtatt agtagtggtt ctgtaaaaat
     5821 ttacctacaa ggttcttata aaaacgatac aaatatcaga gcggatagtg atgttgatgt
     5881 tgtcgttgag cttacaacaa catttggtca taatgcacac gaacttcctt atgaacaaaa
     5941 aaatgctcat gatcaagctt acagtagtgc tgtttatgat tggcaagatc tacgacgcga
     6001 tgttatttcg gcactcgaaa actattacga cgtggctagt gttgatacaa gtggaaataa
     6061 atctatcaag attttgccat catcaggtcg tttaaaagct gatgtggtac ctgtaattaa
     6121 ttatcgtaag tatgattact tttatggaac tgatactcat agctctgaac agggagtaaa
     6181 gttttatcac cgaacaacta atagagctat cgtaaactat cctgaacacc attttaaaaa
     6241 tggagcacaa aagcaccaaa acactcaaaa tcagttcaag caggttgtaa gaatactaaa
     6301 gaatgctcga aattatcttg ttgaaaaaaa tcttctagat aaagaaaaag cgccatcata
     6361 ttttctgcaa agtttgattt acaatgtacc aaattcttgc ttctctaatg atttgtctgc
     6421 atccacaaga tctgttttga atcatttgta taccaatcca attgataatt ttatatgtca
     6481 aaatggtctt actccacttt ttggtacatc agaagaacaa tggaacaccg atgacgcggt
     6541 agcaacaata gtcgcacttg ttgatctttg ggataattgg tatgaaagat agataattat
     6601 atgcacccat actcaataaa ttcagaagag cataaaagaa catctttctg tatagccgta
     6661 tttgcaataa tgcttgcttg gggattgtat ctattctcca agaacgtaat gtcattgcct
     6721 tggtggatag aagcaccatc agtaataggg ttctacggaa ttctatacaa atggtttgat
     6781 attaagcttt ggagaaataa acatattcgt aaactacttg gaattagaac acctatagtt
     6841 gatggttctt ggtcgggtga aataaactcg gcttctgttt actcaaaagg accaatacca
     6901 acaaaaaagt tttgtattaa acaaacatgg acacatgtca gtatttactt agagacagaa
     6961 acatctgaat ctcatagttt tgaagcatcc atgaatgtgg atcaatttga tacagcgaga
     7021 attcattatc agtatatgaa tgtcccaaag gatagtgcac ctgaaactat gcatattcac
     7081 tatggatctg cgacagctaa acttactgat gaaaatagcc ttgaaacaga atactattct
     7141 ggaagagata gaaataacac aggttcattt aaggttataa agttacagta gagtgtaact
     7201 ttatatagcc tatggtgtgt aacttttgtc actttttagt ccgcacgaga cgcgtcgatt
     7261 ttaaatctga aaaaatgcta aaataacatt acagggcttg tcggtgtttg gttctaacac
     7321 gacgctctcc gcacaaaaca ttaggtcccg atgcgaaagc atgggggcct tttgttttgg
     7381 caagagggag agggggagaa gggcggaagc gggcaccgtc gccggcggag caaagcgtag
     7441 cggcgcacgg tcgctgagcc ggggctgcga ccttttatga agtaaaagag ttgtagccga
     7501 ctcctctacc ctccgcacaa aacattaggt cccgatgcga aagcatgggg gccttttgtt
     7561 ttggcaagag ggagaggggg agaagggcgg ataacgtcat gatgaatccg aagtacgacc
     7621 agaacgaagt catgctggat ctccagttcg tcgcgaagaa cggcggacgc gagaagatcg
     7681 agtccgagct cgcgaagctc gccgactacg agaagcgtct cgcctcggcc aacctcctca
     7741 agtacgaggt cgacctcgac gtcaccccga agtgccccgg caacctcaag atcgccgagg
     7801 agaagcacca gattgcctcc cgcgtgaagg ggaagatcga ggtcggctcc gcagacgacg
     7861 tcaagaagct cgtcaacctc cacctcgacc cgggccagat gaacggcaag tgcatcggca
     7921 gtcacaagct gaagaagaag ctggagggac agccggtcta cggggccgtg ctcctcgact
     7981 tcctcctcga gcaccccgag ttcatccccg acgagtggaa gacgaagggc ctcatctact
     8041 tctggggcac gaccttccgc agctccggcg gcggcctgtg cgtccggcgc ctcgattggg
     8101 gcggcggcag gtggtactcg gacttctact ggctcgacta cgtctggtac ggccacgggc
     8161 cggctgctct ccgcgcgtag gcatctcagt ccttagttct taaatcacag cgagcgcctg
     8221 gtaattccgg gcgctctttt cttttattta aaatatattt aaagaaggac tgagacgccc
     8281 tttactcttt tgtcttaaat tttttatcct taattttcct ttttcctttt taaaaatttt
     8341 ggcgggtgaa aatttgtgat agtataactt tgacgatgag tgaatatgtg agagatgtaa
     8401 aaagtttttc gcgagcatag atcatgagac gttgcttcag atatcggctc ggcatattga
     8461 tgacagggac gctttgtggc tcatggggcg agtgatatca agttttcatt ctacgcgcga
     8521 aggcgtagga cttcctctcg gtaatctgac ctcgcaacta tttgtgaata tctatatgaa
     8581 tgggttcgat cagtttgtga agcataaact gaaagtgaaa tattatgtca gatatgcgga
     8641 tgattttgtg atattgtcgg aagaccggga atctttgaat aatgtgctta tcgtaatggg
     8701 agatttctta aagagaagac tccgtctcaa tcttcacccg gacaaggtgt ttatcaaaac
     8761 gcttgcttcc ggcgtagact ttctcggctg ggtcaatttc ttcgaccata gggttcttcg
     8821 ggcggggacg aagaggagga tggtgagaag aattgatgaa aaagcgtctc gtgagactat
     8881 aaattcgtat ttggggatga tgaaatatgg gaatggatat aaaagcacga agaaaatatt
     8941 ttgtaattag ttgctcgctt ttttgtaggg aaaaatgtat atttccctca tgcgcctttt
     9001 gggcattgat tatggaacta aaagagttgg agtagctttg tctgacgaag cgggtctttt
     9061 tgcttatcca tattctgttt taaaaaatag caaaaatctc tcaaaagaaa ttattgagat
     9121 atgcaagaaa gaaaaagtcg ggaaaataat tcttggagaa tcgttagact ataaaggtaa
     9181 atgcaacaat atcgtctgcg agacggatat tctaaaaaat attttggaga aagaaaccgg
     9241 gctatcggtc atttacgaaa acgaagtttt gaccagtgcg gaagcggaaa ggatccaggg
     9301 taagactgaa atgttggatg cggccgcagc tgcgataata cttagaactt atatagatag
     9361 gcagaaaaat aactaatagg ggataaacaa acttttcccc aatgacgcta ctttaggtag
     9421 tggtataatg ttattaatgg ccaaaaaatc tttttttgat ctttttccgg ttcccgagta
     9481 tttatcgtat cccgctgtcg ggctggatct ttgcggttat gcgataaagt ttattgaatt
     9541 tagccggaat agcggaaaat taaaaataga aaaatatgcg aggcgaccga tatccgaaac
     9601 ccttccctcc aattctccgc aggaacaagt agatgaggct attattaata ctcttatagg
     9661 cataaaagaa gagtttggaa ttcgcgcggc gcacatttct ttgccggaag aacactctta
     9721 ttttctgaaa ttgaaattgc ccaagaccga agccaaagag ataaggagca gtatagagtt
     9781 acagcttgag gagcatgtgc cgtattcccc ggaggaaata acttttgatt atgaagttat
     9841 caatactttg gaaaatggag aggtggaaat cattgtttcc gtgcttccct ctagcgttat
     9901 agacaggtat gtaaatataa taaatagagc gggaattaca ccaatatctt ttgagacaga
     9961 agcggcttct atgatgaggg ctgtcttgcc gcaagatttt acgaagtcgg cgcttgtcgt
    10021 ggatatcggc aaaacaagct caactctttc tatcgtaagc aaagggattg tttggtttag
    10081 ctatactatg aagacgggcg gggcgctttt tactaaggtt gtttccgagg ctttatctgt
    10141 atcagaggag gaagccgaaa agataaaatt taacaagggg cttattagat ctccggaaaa
    10201 tcaagaggta ttgtttgctc tagctccggc catatcaagt ctccgagacg agattattaa
    10261 acattattgg tattggggtg cccacaaaga aatttccaat caaggagaga tagaaaaagt
    10321 ttatctttgc ggaagccagg cctctataaa tggccttgcc gagtatcttt ccgttagctt
    10381 aaacttgccg gtatttttgg cgaacccgtg ggaaaatgtc ttttcttttg aggaaaatat
    10441 tcccgagctt cctttggcta gttcccttga atatgtaacc gctatcggtt tggcgttaaa
    10501 gggtgtgcag gataataata agaaataaaa tgaatctcct tcctttaaat gaaaaaaaac
    10561 aagccagatt tgaatatcta ctgagatttt ggactgtcgc cggaatggcc acgatagttc
    10621 ttttggtcgc cgccgcaact cctctcataa tttcatattt tacgatttct tataatgttg
    10681 attttataaa agacgtcgca gagaaccttt caaaaagtga aggattcaaa aagacagaca
    10741 ctgtcgccaa gattattaag gatgttaaca gaaaatcagc tatccttaac gaccctctca
    10801 gcactactgc gaggcaggat cttttgagtt ctttctcttt tgtttttaag acagccgaca
    10861 aaatgcggat aaaccaaaag gggattgtta aaataaaaca gataatttat gagcaggtag
    10921 tcaaaaaggg ttcagctcga acaggaagcg tggcgtccac cacggcctcc gccacatcag
    10981 agtccggtat tcacaaaata aatataaaag gtttttctgc gaccagagac ggatttttat
    11041 cttttttgaa ggagctagag tctaataaaa acttcgtaat gatagattct ccggtttcca
    11101 atttggtaaa tagcgaaaat ctggattttt ctttgactat aacattaaaa gataaagcga
    11161 tatgacggat atcaaacaaa aactaaaaaa gaagctctac atcgtcatcg ttgctttagc
    11221 ggtaaccgtt attttttatt tttatttatt tagcgtggtc agagaaaagc aaaataccgg
    11281 gctaaatagt gcgatttccg acctcggccg gcttgaatcg aagcaatcta atttttctct
    11341 taacagtaga atcttggacg aaacgacggc agaaagagcg cgattgaatt cttttattct
    11401 agataaaaaa accgttgccg attttattca acaaatagaa gatttgggta agcatgcaaa
    11461 tgtagaaata accaaatctt tgtctgtcga aaaaaatccg cagaagccaa aagaaaatat
    11521 actaaaattt aatgttaggg cgaaaggaac tcttaaagat gtttattatt tcactctttt
    11581 gacggaaaac cttccttata aaataagggt taaaaaaatg gcgataagca cgagcgacgc
    11641 cgacacgtct tctttctcgt cgggggttat gggcgtatta gagggtaaga aaaaaactgc
    11701 cgccgttccg agtgtttggg tgagtgatat aaattttgaa gttataagtt atataaatga
    11761 ataaactttt tgcaaaatta aatatttttt cacctttgcg aaaatttttt gacaaagacc
    11821 ctgaaaaagt tgagccttcc cctcgttttg gttgggtgct cattttgatt ttattcctga
    11881 tgttttctct ggcgtttatg gttctctatt attttatgtt tttgccgcag tctgatgagg
    11941 cgctttccgg caagccggct tttgatccgg aattatccat tgaaaatatt tccaagccga
    12001 aattacaaaa aactttggag ccttggatcg ccagagagaa gcagtttaat gattatctca
    12061 ataatcgtcc tagctttgac tttttgaagt aaattgtgta ttgttttctt cattcccgag
    12121 gaattgctct cgtagtttca gccaaaggct gatccgcccc tggcggacaa tgtatctgag
    12181 cattatcgat gatatttgtt taatttaaag aaggtttttg tccatattct gctctcgtag
    12241 ttcaatggat agaacaggag acttctaatc tcctgatgta ggttcgattc ctaccgagag
    12301 cataaaacga ccaccgaaat gcgtaagcat ttcggtggtt ttcgttttat agctctcggt
    12361 aggaatcgaa ggccgcagtc gggcccaccg ctgtaagcgg gggagacgag gcgggctcgc
    12421 gggtacttag atttttataa gcgaagcgac ataaaaagct tagtaacccg tgagcgattc
    12481 ctaccgattc cttcctttta atgcgcaagc attcgggcgg ttttcgtttt atagctctcg
    12541 gtaggaatcg aaaggcggaa gcgggaccct ttctgcaaga aagggagctg agccggggcc
    12601 gagagtactt atgagcgaag cgaattagta actcgtggcc gattcctacc tttttggaat
    12661 cgaaagtttc tttttatctg tgctataatc gccatatgga aaatatggaa caacaaaatc
    12721 aaaaatctag agaatggaga attgtcggaa aggcttcttt ggggaaaaag aatgaattta
    12781 aagagaagat gaataagaaa ttgtttgcac agaccgaagc catggaaaag gaattaaaac
    12841 aagaacagaa agacgaagtg aaggcggcgg agatagaaaa gactgcggag cagataaggc
    12901 taattgaatt ggcaaatgaa gataccaata aattgatgga gaggcttggg tttgagccga
    12961 ttgatatttc tcctgacaat gtttacgtaa ttaaatcgga atcattctct aaattaaaag
    13021 ggccgactgt aaatgctttt gccaattaca ataaccaggc tattgtcatt aatggcgaaa
    13081 agcccagatt agtacaaggg ggaggcgcca tcttctgtac gctgactttt catgagatgc
    13141 ttcacttgaa gagtcatatt tcttttgaag cgcagtcaga caaaagaaaa ccagagctgt
    13201 ttagagccgg ggtgacagta tattcctcag ttaagtcaga caaagagggt atgatgcacg
    13261 gtcattttcg tggcttaaac gaggcgattg tggcactcgc tgaacaggat gaattgaaga
    13321 atattttggg aaccgatgaa tttaaagaag aagggagttg gcaagattca gtgctggcaa
    13381 aagacgtcaa ggaggatatt ccgtctataa aagataaaga tgcgattttc gttctaagaa
    13441 aaaaaataga aggtcaggtg cccaaaactc tatttgtgtc agaaacgcta tcttatacaa
    13501 aacataaaga gactttaaaa tatttaatgc aggagatttt gtgggataac caagataatt
    13561 ttatctccga agatgaagtt tttgatgttt ttttgagagc gcatttttct ggaggacttt
    13621 tagaaattgc caaattagtt gagaggtcct ttggcgaggg aagttttaga cagttagggg
    13681 aaatgacaga tagcgcatcg tctgctgtat caacgttgga attattcaaa aaactccgcg
    13741 ggaagattct gcgagaaaga gagaagggcc agaaataaca tctttttatt tgtgcaggat
    13801 ggaagactcg gctacaactc ttttgctgcg caaaaggtcg cagccccggc tcagcgaccg
    13861 tgcgccgcta cgctttgctt cgccggcgac ggtgcccgct tccgcccttc tcgtctctta
    13921 ccctcgctac aaaataaaac cacctaaatg ctatcgcatt taggtggtta ttttgtgcgc
    13981 ggtaagagac tcgaactcct gaccttcccg gtgtaaacgg gttgctctac caactgagct
    14041 aaccgcgc
//
LOCUS       DAOTEX010000014        13792 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3118
            NODE_3076_length_13792_cov_4.597947, whole genome shotgun sequence.
ACCESSION   DAOTEX010000014 DAOTEX010000000
VERSION     DAOTEX010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868142
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 13792)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13792)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 883
            CDSs (total)                      :: 833
            Genes (coding)                    :: 829
            CDSs (with protein)               :: 829
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13792
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3076_length_13792_cov_4.597947"
                     /isolate="AMR_MDS_3118"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..507
                     /locus_tag="P5056_01450"
     CDS             <1..507
                     /locus_tag="P5056_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62416.1"
                     /translation="KNGYFLTCHHCVNPLGDLLLLDNHGKVIEIEGVCLQDETTDIAL
                     VKAKIAGPREIIKYKYAQYLDTNEQIAILSRWMGKFSVTCGFVKYGICREKETGRFSL
                     AVSQISKKGDSGGVVANMRGEILSIHKSASEITNTSPGAPTGPASISVPFFYITEFLE
                     KNLFLREV"
     gene            511..684
                     /locus_tag="P5056_01455"
     CDS             511..684
                     /locus_tag="P5056_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62417.1"
                     /translation="MFYQVMIFCKNSEKALQEAINSWLKENHSIILGGPPTISFSCDR
                     GEKIAIILYKTAD"
     gene            798..1841
                     /locus_tag="P5056_01460"
     CDS             798..1841
                     /locus_tag="P5056_01460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HRY62418.1"
                     /translation="MLNTFLFVALGICTGIIIAYFLFKRNDTNHEESGGLMLIQNQIN
                     ELAKRLDKRMEESQTALSRSMNTQFSHIKDITESLVRLDETNKQVVNFAGQLQNLQDI
                     LKNPKQRGILGEYYLESVLKNVLPPDRYEMQYKFNGGDIVDAVIFLDKDKILPIDSKF
                     SLENYNKIIEEKNPAEKDKLEKLFRQDLKNRIDETSKYIKPEEGTMEFAFMFIPSEAI
                     YYDLLINQVGAIKASTKDLIEYAFQQKKVIIVSPTSFMAYLQTVLQGLRALQIEESAK
                     EIKKRVEDLGKHLLNYDQYMKKVGDTLQTTVNHYNKASGEFKKIDKDVLRIGGEPAGL
                     SDSLIEAKIENIG"
     gene            2008..2334
                     /gene="rplU"
                     /locus_tag="P5056_01465"
     CDS             2008..2334
                     /gene="rplU"
                     /locus_tag="P5056_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009352881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HRY62419.1"
                     /translation="MKFAVIQTGGKQYIVKPGDVIKVEKLTENDKLVPEGGKIKFDEV
                     LLVDDEGNTKLGTPFINGSSVSSVVVSNGRGRKVITQKYKAKVRFRNKKGHRQPFTQV
                     RIEEIK"
     gene            complement(2454..3062)
                     /locus_tag="P5056_01470"
     CDS             complement(2454..3062)
                     /locus_tag="P5056_01470"
                     /inference="COORDINATES: protein motif:HMM:NF025044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HRY62420.1"
                     /translation="MDPITQLTEIFSHFPGIGPRQAKRFVFYLLGQNDSANKKIADLI
                     LELKKDIHSCVRCMRFFTDNKKGALCGICSDEGRDKTKLLVVAKDVDLDNIERSRSYE
                     GLYFVLGGLVPVLDKNPEERIRQFELKREIERNQELKEIIVALSATVEGDNTADYLRQ
                     IFSPITAARGIKISVLGRGLSTGTELEYSDMDTIKNALKHRE"
     gene            complement(3181..4575)
                     /gene="dnaB"
                     /locus_tag="P5056_01475"
     CDS             complement(3181..4575)
                     /gene="dnaB"
                     /locus_tag="P5056_01475"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HRY62421.1"
                     /translation="MFMVQGIKNNLRTPPHDTDAEKAFLGSILLRSQSIPDVADMLDP
                     DSFYSGKNSVIYKTIFDLYTKGEPIDLVSLSSRLKEKGQLEQAGGASYLAELINTVPS
                     STNVEHYAKIIQKKSLLRKLIESGDHVSELGFNEHREIEDVLVEAEKKILGVSGSLLK
                     NKFIPIRNTLTEAWERLDRLHNSSDEIRGIPTGFKGLDNKLAGLQKSDLIILAARPSV
                     GKTSLALDLARQAACKYNVPVGIFSLEMASQQLADRMVAAESGVDSWKLRTGKISSDE
                     EFIAIREAMDKLNKAPIHIDDEASNNILRMRSVARKLQRDQGLGLIIVDYLQLMVPQR
                     HSDSLVQQVTEISRSLKILARELDVPVLALSQLNRQVEQRGGPPRLSDLRDSGSIEQD
                     ADVVLFIHREDKNDKNAPPNIAKIIIAKHRNGPTGETELFFDAERVSFMDLDKSAGGD
                     ESSSPSDNAGFGDF"
     gene            complement(4740..6479)
                     /gene="thrS"
                     /locus_tag="P5056_01480"
     CDS             complement(4740..6479)
                     /gene="thrS"
                     /locus_tag="P5056_01480"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HRY62422.1"
                     /translation="MDNENKLHNTRHSLAHILAMAVLKKDPKAKLGIGPVIDNGFYYD
                     FLLSKPLKDEDLKDIEKTMKKIVSQNLEFTAKEVSRSEAEKIFGDEPFKMELIKELSE
                     ADSKLSVYTSGEFSDLCKGPHVKSTKEINTDAFKLDKIAGAYWRGSEENQMLTRIYGL
                     AFETKEELDAHLNMIAEAEKRDHRKLGKELKIFTLSPLVGSGLPLLQPNGMIIRNEIE
                     RYLWELHKTKGYKRVWTPHLAKEDLYVTSGHAAKFGDELFRVTGKEEKFFLKPMNCPH
                     HMQIFADNQFSYRDMPVRYFEPATVYRDEKTGQLAGLTRVRSITQDDGHLFCRINQIN
                     QEVSTIVSIIKEFYTTIGMMNDYWVRLSIRGPEGKYLGGDDVWNTAEAALREAAELNK
                     LPFRVGIGEAAFYGPKLDFMFKDAIGREWQLATIQCDFNLPERFSLEFTNEKGEKERP
                     VVIHRAISGSLERFMGVMIEHYAGHFPTWLAPVQVSILPISEKYLRYAEVIRDALADA
                     DVRVELDYSNETLGKKIRESKKNKIPYMLIVGEKEAMSGMVTVESRKGSLGALKIEDF
                     ISEISKEIRERKA"
     gene            complement(6488..6772)
                     /locus_tag="P5056_01485"
     CDS             complement(6488..6772)
                     /locus_tag="P5056_01485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62423.1"
                     /translation="MTNIENFDPTQKERAEDKEATLLLNAAEKEKKIALIELNDAKRR
                     GDPKNRIETLEHIYERAVKGFDELLEKRRSADARASAQKKKFVDEEPVLK"
     gene            6900..6971
                     /locus_tag="P5056_01490"
     tRNA            6900..6971
                     /locus_tag="P5056_01490"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6932..6934,aa:Pro,seq:cgg)
     gene            7343..7822
                     /locus_tag="P5056_01495"
     CDS             7343..7822
                     /locus_tag="P5056_01495"
                     /inference="COORDINATES: protein motif:HMM:NF014373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HRY62424.1"
                     /translation="MIDASTIEIFIQLTVAMLLGMTLGIERTIAHKTAGMRTYALISM
                     GSAFFVIISEIMSAKYALMPAINPLLLPAQIVVGIGFIGGGIIFVQGDIVRGLTTAAS
                     LWVTAAVGMATGFKMYAPAIFVTIVTLFVFKVLWQIEDRIKHFVGKNDECKIDHSGK"
     gene            8007..12791
                     /locus_tag="P5056_01500"
     CDS             8007..12791
                     /locus_tag="P5056_01500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62425.1"
                     /translation="MATFNKKRAVIFVIAFVVSSSFLGVFDVAEASFNKLKDTQNLSG
                     TKEVITGIGADIMKSLGEAGARLKDSLKYFSQKTSAKINFDKNLAQTSEPVGNFFTRS
                     VNYITDIINNFFASPSTTPNLLPAPTQNETTETNVPAVVSPGTQQTPQATTKVIERII
                     TTTPAPVTQPLVSSLGFYTKEEMDAVLRNYDQTTKNDLISRINNLSASTNSQYAATNM
                     AVALTNKIDSLSNVTLTNVTVKGASGLEDSDIPDGITASNYLLLAGGNITGNLGIGTT
                     SPYAPLSVNGEAAVAYLTTSSSTATSTFVYGINLKGGCFAIDGVCVTGSGLGGTADGA
                     WSTTSADFWETTQAPRGVGFSTTSADYYLSQNQGAAFSTSSADYNFSANLSATNTGAI
                     AEGSNKYYTDDRVAGVIAATTTDALTQGATNKYYSTNLLAADLAATTTTALAEGDNKY
                     FTNDRVAAVIAGTTTTAIAEGEKLYFTDTRADDRAISVLSATTSLPNVSTLSGLVTFG
                     STTATTTGEGNLKITGNAELGTVTSGAWNGTAIGDAYLTKTGDWTGTFDGQEGSYYLD
                     ARNLTNFGSPFFSFFSATTTDALAQGSTNKYWSQTLFDNALSATTSLPNITTLASLSL
                     PVTQLSGNYDAKNLANFGTPFFSFFSATTTDALTQGSTNKYWSQTLFDNALTATTTLP
                     KITTLSSLSLPVTQLSGNYDAKNLANFGTPFFTYFNGTTTDALAQGSINKYYSTDLLA
                     ADLAATTTTALAEGDNKYFTNDRVAAVIAGTTTTAIAEGEKLYFTNTRADDRAIGVLS
                     ATTSLPNVSALSGLATFGSGTATTTAEGHLKVTGNTELGTVTAGIWNGTAIGDAYLTK
                     TGDWTGTFDGQEGSYYLNAKNLTNFGSPFFSFFSATTTDALAEGSINKYWSQTLFDNA
                     LTATTTLPKITTLLGLTNATTTNLTITSNAYFPSGIWNSSGNVGIGTSNPTHDLETYT
                     SGGAAMYLGHSNASGSFPKVSAIGLGSNAVSNSYSTNGNTMSVVGSAQIAAIQSQSSG
                     AETDLALYTATGGSVTERMRINSAGNIGIGTVAPANVKLEVSGAATADTNGNGVSLTQ
                     TLTDTTAFATGVGGGLGFAGYYTANPDRAVWSAVKGFKENSTTGNYAGALSFYTRANG
                     GSLTEKLRIDSTGNIGIGTTTPAQALTINSTVSGIAPKLQILGGTPLSVGNVTAAVIL
                     GMNEGAVAQDGVELQAVRSAWVNPNATDFAISVAGSERVRVDKTGNVGIGTGAPGAKL
                     TIAAGAFQTDTENIRFNRTEDSARYNSIYSRSDATAAANNIAFKVHNAVDTTSQTEVM
                     RLLGNGNVGIGTTNPDAKLDVAGLIKTEGALNSFRNYYDATHFSEITAKSNGDLQMYG
                     FNGATYQNILLAMDGNTQAGNVGIGTTNPTHKLETYSDNGVSAYLGHSNTTGSFPKVS
                     AFGLGSDAVTKGYSNNGGTLTVTGSAQIAAIQSQSSGAETALAFYTTTGGSVAEKMRI
                     GNTGNIGIGTTNPQGRLHIGSTTYSTLSPSYQFNMFGPLNIMQRDAATDSYVTNNAFF
                     NSGNQWQRVLAGGAGRIGWDSDAVGAMDFKVAPTGTAGSTFS"
     gene            12801..>13792
                     /locus_tag="P5056_01505"
     CDS             12801..>13792
                     /locus_tag="P5056_01505"
                     /inference="COORDINATES: protein motif:HMM:NF025253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098024 - virus tail, fiber [Evidence
                     IEA];
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - viral entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HRY62426.1"
                     /translation="MTILGTGNVGIGTTTPDTKLSVNGTTKFGNGVWPELAIGMSGTR
                     MGVLNDTENPSLIMSNMSNTAAANNGSEIYLGARKTTGLADWVGGMISGYKEFASGVE
                     GGYLAFKTVNSAGTLTERMRINTSGSVAIGTTTPQAELHIEGGSFNTRLLVNQSGADG
                     GMMNAFMGVQVSAFSDTSGVIRFKDKGANVAANEFFVIENSNASNDAAAGLLNVKNNA
                     GSILSVKHGGTIFASASLGAESGGDNYLCIDPTTHEITNGGADCGASSERYKENIKDL
                     GYGLKDVLKMRPVSYKFRETGRDSIGFIAEEVINIIPEIVETKDGLPESIDYNK"
BASE COUNT         4019 a         3097 c         2994 g         3682 t
ORIGIN      
        1 aagaacggct atttcctgac ttgtcaccac tgcgtaaatc cgcttggaga cctcttgcta
       61 cttgataatc acggtaaggt gatagagata gagggggttt gtctgcaaga cgaaaccacc
      121 gatattgccc tcgtgaaagc gaagatcgcg ggaccaagag agataattaa atataaatat
      181 gctcaatatt tagacacgaa tgagcaaatc gctatcttgt ctaggtggat gggtaaattt
      241 tccgtcacct gcggttttgt aaaatacggc atttgtcgag aaaaggagac gggaagattc
      301 agcttagccg taagccaaat aagcaagaag ggcgattccg gcggagtggt agcaaatatg
      361 cgaggagaga tcttgtctat ccataagagc gcctcggaga ttaccaatac ttccccagga
      421 gcaccaactg gtccagcctc tatttctgtt cctttctttt acataacaga gtttcttgaa
      481 aaaaatttat tcttaaggga ggtgtgaaat atgttttatc aggtgatgat cttctgcaaa
      541 aacagcgaga aggccctgca agaggcgatt aacagctggc ttaaggaaaa tcacagtatt
      601 atccttggtg gacctccgac gattagcttc agctgtgaca ggggagaaaa gattgcaatc
      661 atcctctata aaaccgccga ctaaccgacg ccgaacctta atcccgacca tcatggtcgg
      721 gattttttat cctgtagtag aggcgcgccg cgccttcact acgggatttt tttaccccaa
      781 aatgttaaaa tatgtttatg ttaaatacat ttttatttgt agcgcttggt atatgcaccg
      841 gcataataat cgcttatttt ttatttaaaa gaaacgacac aaaccacgaa gaatccggcg
      901 gtcttatgct tatacaaaat cagataaatg agcttgcgaa gaggcttgat aagagaatgg
      961 aggaatctca gaccgcgcta tctcgctcca tgaacacaca atttagccac atcaaggaca
     1021 taaccgagtc gctagttcgg ctagacgaga cgaacaaaca ggttgttaat ttcgcaggac
     1081 agctccagaa cctacaagat attctcaaaa accctaaaca aagaggaata ttgggcgagt
     1141 attatcttga aagcgtctta aaaaatgtat tgcctcccga ccgatatgaa atgcagtata
     1201 aatttaacgg cggcgacatt gtggatgctg tgatattttt agataaagac aaaatccttc
     1261 cgatagactc aaaattctcg ctagagaatt acaacaaaat aattgaagag aaaaatcccg
     1321 ccgaaaaaga taaattggaa aaacttttta gacaagatct aaaaaacaga atagacgaaa
     1381 cttcaaaata tattaaaccg gaggagggga cgatggaatt cgcttttatg tttattcctt
     1441 ctgaggcgat atactacgac ctgcttataa atcaagtcgg ggcaataaaa gcatctacaa
     1501 aagaccttat tgaatacgcc ttccaacaga aaaaggtaat cattgtttcg ccgacttcgt
     1561 ttatggccta tcttcagact gtcttgcagg gtctccgcgc cctacaaata gaagaatccg
     1621 caaaggaaat aaagaaaagg gttgaagatc tgggcaagca cctcttaaac tacgatcaat
     1681 atatgaagaa ggtgggcgat acgctacaga ctaccgtaaa tcattacaat aaggcctccg
     1741 gcgagttcaa aaagatagat aaagacgttc tcaggatagg aggcgagcct gcagggctat
     1801 ctgacagcct tattgaggcc aaaatagaga atattgggta aaatctttag tttagttgtc
     1861 attcccgtga aaacgggaat ccagagaaga ggtagataga tccccgcctt cgcggggatg
     1921 acaaaaaaca ttaaaaaata ccgtgcccct tgcatttttt gtcgttttta tgtagtatgt
     1981 ccttactata aaaggtaaat ttaaaacatg aaatttgcag tcatacaaac aggcggaaag
     2041 caatacatcg ttaaaccggg cgacgttatt aaggttgaaa aattgaccga aaacgacaag
     2101 cttgttccgg aaggaggaaa gataaagttc gatgaggttc tccttgttga tgatgagggg
     2161 aatacaaaat taggaactcc atttatcaat ggttcgtcag tatctagcgt tgtggtaagt
     2221 aacggaagag gaaggaaagt aatcacacag aaatataagg ccaaggtcag attccgaaac
     2281 aagaaaggcc acagacagcc attcacacag gtaagaatcg aggaaataaa ataataaaaa
     2341 aacaccccgc cttttagcgg ggtatttttt tattaccccc gtgaaaacgg gggtccatag
     2401 aacacaaaat agattcccgc ctacgcggga atgacaaaaa gagtaattat tttttactct
     2461 ctatgtttaa gagcattctt tatggtgtcc atatcggaat attctagctc tgtgcctgtc
     2521 gaaaggccgc gacccaaaac ggaaattttt atacccctag cggccgtgat gggcgagaat
     2581 atttgtctaa gatagtcggc agtattatct ccctccaccg ttgcagaaag cgcgacaata
     2641 atctccttaa gctcctgatt tctttctatt tctctcttaa gttcaaactg ccttattctc
     2701 tcttccggat ttttatctaa tacaggcacc aatccgccaa gcacaaaata tagcccctcg
     2761 tagcttcggg acctttcaat attgtccaaa tctacatctt tggccacgac aagaagcttt
     2821 gtcttgtccc taccttcatc gctacatata ccgcacagtg ctcctttttt gttgtcggta
     2881 aaaaacctca tacatcttac gcaagagtgt atgtcctttt ttaattctaa gataagatct
     2941 gcaatttttt tattcgcaga gtcgttttga ccgagcaaat aaaaaacaaa gcgcttagcc
     3001 tgtctcggcc ctatgccggg gaaatgtgag aatatctcgg ttaattgtgt aattggatcc
     3061 atttttgtca ttcccgtaaa aacaggaatc tttttatatt actggattcc cgctttcgcg
     3121 ggaatgacac cgtatttaaa gtatctaact acattctaat ttcaaccccc tactctctaa
     3181 ctagaaatct ccgaaacctg cgttgtccga tgggctggaa gattcatctc caccggcaga
     3241 tttatcaaga tccataaagc tgaccctctc ggcatcaaag aatagttctg tctctcccgt
     3301 aggaccgttt ctgtgcttag caattataat cttcgcgata ttcggcggag cattcttgtc
     3361 gttcttatcc tctctgtgaa taaacaaaac cacgtcagcg tcttgctcga tggaccctga
     3421 atctctaagg tcagaaagtc gtggaggtcc gcctcgttgt tcaacctgtc tgtttagctg
     3481 agaaagcgcc aggaccggca cgtctagctc tctggcaaga atcttaagag atcttgatat
     3541 ctctgtaacc tgctgaacta gcgaatcgga atgcctctgc ggaaccataa gttgtaagta
     3601 gtctacgata ataagcccga gaccctgatc tctttgtagc ttcctggcca cagatctcat
     3661 cctaaggatg ttgttgctgg cctcgtcgtc tatatggata ggcgccttgt taagcttgtc
     3721 catcgcctcc ctaatcgcga taaattcctc atcgcttgat atcttgcctg ttcttaattt
     3781 ccaagaatca acgcccgatt ctgccgcaac cattctgtcg gccaattgct gagaagccat
     3841 ttctagagaa aatataccta ccggaacatt gtatttgcaa gcggcctgcc ttgcgaggtc
     3901 tagcgcgagg ctcgtctttc caactgacgg tctggctgcg agaatgatga gatcagactt
     3961 ttgaagaccg gcgagtttat tatcaagccc cttgaaaccg gtagggatgc ctctaatctc
     4021 gtcactagag ttgtgaagcc tatctagcct ttcccacgcc tcggtcaaag tatttctgat
     4081 aggaataaat ttatttttaa gcagggaacc gctgactccg agtattttct tttcggcctc
     4141 caccaacacg tcctcgatct ccctgtgctc gttaaatcca agttctgaaa catgatcacc
     4201 ggactcaatc aacttcctca gcaaactctt cttttgaatt atttttgcgt agtgctcaac
     4261 attcgtagaa gaggggacgg tgtttatgag ctcggctaaa tagctagctc caccagcctg
     4321 ctcaagctgg cctttttctt tgaggcgcga agaaagagaa acgaggtcaa tcggctctcc
     4381 ttttgtatat agatcgaaaa ttgttttata gatgacggaa tttttgccgg aataaaaaga
     4441 atccggatca agcatatccg cgacatcagg aatgctctgc gatctcaaaa gtattgatcc
     4501 caaaaaagct ttttcagcgt cagtatcatg cggaggagtg cgaagattat tttttattcc
     4561 ctgtaccata aacataattt agcacgagcc gacattctaa taaaatattt tcgggtgata
     4621 aacatgttga tattgtgggg atgaaacacc gaagcgaaaa aataaaagcg aaaagcgcaa
     4681 aataacggcc taaaatcaga aaatttagac ttctgtcttt ttgctttaca cttttcgttt
     4741 tacgctttgc gctctctaat ttctttagat atttcagata tgaaatcctc tattttgagc
     4801 gccccgaggc ttccttttct agattctaca gttaccattc cgctcatggc ttccttttct
     4861 ccaacaataa gcatataagg aattttattt ttcttgcttt ctctgatctt tttgccgaga
     4921 gtttcgttgc tatagtctag ttctacgcga acatcagcat cagccaacgc gtctctaatt
     4981 acctctgcat atctaagata cttctcgctg attggtaaaa tggatacctg cacaggagca
     5041 agccaagtag ggaagtgtcc ggcataatgt tctatcatca cacccatgaa tctctcaagt
     5101 gaaccggata ttgccctgtg tatgacgacc ggcctttctt tttctccttt ttcgtttgta
     5161 aactccagac taaatctctc ggggaggttg aaatcgcact gtatcgtcgc caactgccac
     5221 tccctgccta tcgcatcctt gaacataaag tctaattttg gtccgtagaa agcggcttct
     5281 ccgatcccga ctctaaatgg aagcttgttg agttccgcag cctccctgag cgccgcttcg
     5341 gcggtattcc agacgtcgtc accgcccaaa tatttgcctt caggtccgcg tatagagagt
     5401 ctcacccaat aatcgttcat cattccgatt gttgtataaa actccttaat tatagaaacg
     5461 atagttgaaa cctcctgatt tatctgattt attctacaaa acaggtgccc atcatcctgc
     5521 gtaattgatc tgactctggt aagaccggca agctgtcctg ttttctcatc tctgtaaaca
     5581 gtcgcgggct caaagtatct caccggcata tctctatagg agaattgatt atcggcaaat
     5641 atctgcatgt ggtgcgggca gttcatcggc ttcaagaaga acttttcctc ctttcccgta
     5701 actctaaaaa gttcatcacc gaactttgcg gcgtgaccgg atgtaacata aagatcttct
     5761 ttcgccaagt gcggagtcca aactctcttg tatccttttg tcttgtgaag ctcccaaaga
     5821 tatctttcta tctcatttct tataatcatt ccgtttggct gaagtagagg aagacctgag
     5881 ccgacaaggg gggaaagtgt aaaaattttc agctccttgc ccaattttct atgatctctt
     5941 ttttccgcct ccgcaatcat attgagatgc gcgtccaatt cttcttttgt ttcaaacgca
     6001 aggccgtaaa ttctcgtgag catctgattt tcttcactgc ccctccaata tgcacccgca
     6061 atcttatcca gcttaaaagc atccgtgttt atttcttttg tgctctttac gtgaggcccc
     6121 ttgcagaggt cggaaaattc tccggaagtg taaacactca gttttgaatc ggcttcggaa
     6181 agctctttta taagctccat tttgaaaggc tcatcaccga atattttctc cgcttcgctc
     6241 ctcgaaacct ctttggcggt aaactccaag ttctgagaga caatcttctt cattgtcttc
     6301 tcaatgtctt tcaagtcctc atccttaagc ggttttgaaa gcaaaaagtc gtaataaaat
     6361 ccgttgtcta tgaccggacc gataccgagc tttgccttcg gatctttttt caacaccgcc
     6421 atggccaata tgtgcgccaa agagtgtctt gtgttgtgta atttattttc gttatccatt
     6481 gtttctttta ttttaaaacc ggctcttcgt ctacaaattt tttcttctga gcactcgctc
     6541 ttgcatctgc gcttcttctc ttttctagaa gttcatcaaa acccttaacg gctctttcat
     6601 atatatgctc caaggtttca attctgtttt ttgggtcacc tctgcgcttg gcatcattta
     6661 attcgatcaa ggcaatcttt ttttctttct ccgccgcgtt caacagtaac gttgcctctt
     6721 tatcttcagc tctttctttt tgtgttggat caaaattttc tatatttgtc ataagcattt
     6781 gcccgagatt cccgggataa tatgatttat aatcctaatg cgtgaaattc tagcacaaac
     6841 cggcctaaaa ttaaataata tttgaataaa gagcgcatct tgctattata ttcttgtagc
     6901 gggacgtggt gtaacggtaa catacacgat tcgggttcgt gtgactccgg gttcaaatcc
     6961 cggcgtcccg atagggaagc attcaaagca tactaaatgc tttgaatagg gatttgaagc
     7021 cagattgagc aacttttatg agcgaagcga aataaaaggt ccaatctggg gactgaacct
     7081 gtaaggttca aatcccggcg tcccgataag gacgaagcga ggcgcctgcg cgccttgcgg
     7141 cgggatttaa acagcggagc gttgtcgaac gagcacctgg cgagtgagac agcgagctgg
     7201 tggctaaccc gaggaggcgg aagccgacga gaggtgaagc ctaatcccgg cgtcccgata
     7261 aataactcta ataaataatt tttaacaaat atcagaagtc atattataat tcaaaaatat
     7321 taaaaattta taaatattta ttatgataga cgcaagcaca atagaaattt ttatccaact
     7381 gacagtggcg atgttacttg ggatgacact cgggattgaa agaacaatag ctcacaaaac
     7441 agccggaatg agaacttacg ccctgattag catgggctcc gcttttttcg ttattatatc
     7501 tgagattatg tcggcaaaat acgcccttat gccggcaata aaccctctgc ttctcccggc
     7561 gcaaatagta gtaggcatcg gctttatcgg cggtggtata atattcgttc aaggcgacat
     7621 cgtaagaggg ctgacaacgg cagccagcct ctgggtgacc gccgcagtgg gaatggcgac
     7681 aggattcaag atgtatgctc cggctatttt tgtaaccatt gtaactctct tcgtatttaa
     7741 ggtattatgg caaatagagg atagaatcaa gcacttcgtc gggaaaaatg atgaatgcaa
     7801 gatagatcac tccggaaaat aatcattcgg aaaaaagtaa aaaatatttt ttaaaaaaca
     7861 tcttccggtg aaggtgtttt ttccttttat ttataaacat atttttctaa aagttatcca
     7921 catttttatc gttagattat tatttttttt gatataatta ttacattctc actaaaatga
     7981 ttttttaagt tttgtgagaa agaaaattgg ctacttttaa taaaaaacga gctgtaattt
     8041 ttgttattgc gttcgtagtg tcatcctcct ttttgggggt atttgatgta gctgaggcga
     8101 gttttaataa gctaaaggac acgcaaaacc ttagcgggac aaaagaggtt ataacgggaa
     8161 tcggcgccga tattatgaaa agcttaggag aagccggcgc cagattaaaa gattctctaa
     8221 aatatttttc ccaaaaaaca tcggcaaaaa taaattttga caaaaaccta gctcaaacct
     8281 cagaacctgt cgggaatttt ttcacgcgct ctgtaaatta tattaccgat attataaata
     8341 attttttcgc tagcccatcc accaccccga atctcctccc ggctccgacg caaaacgaaa
     8401 cgaccgagac aaacgttccg gcggtagtct caccgggcac acaacaaact ccgcaggcca
     8461 caacaaaagt aatagaaaga ataataacga ccacgccggc tccggtcaca caaccgctcg
     8521 tttcttccct tggtttttat acgaaggaag agatggacgc cgttctgaga aattatgacc
     8581 aaacaacaaa aaacgacctg atctcgcgca taaataatct tagcgcttcc acaaattcac
     8641 aatatgccgc cacaaatatg gcggtcgccc tcacaaataa aatagacagt ctttccaatg
     8701 taacgttaac caacgtcacg gtaaaaggcg cctccggcct cgaggactcg gatatcccgg
     8761 acggcattac agcatcaaat tatctgttac tagccggcgg gaacattacc ggcaatctgg
     8821 ggattggaac aacatcgcca tatgctccgc tttccgtaaa tggagaagcc gcggtggcct
     8881 atcttacaac atcttcctcc accgccacct caacgtttgt ttatggaatc aacttaaagg
     8941 gtggttgctt tgctattgat ggagtctgcg ttaccggtag cggacttggg ggcacggcag
     9001 atggtgcgtg gtcaacaact tccgcagact tttgggaaac gactcaggcc cctcgaggtg
     9061 tcgggttctc tacaacttca gcagattact acttgtccca aaaccaaggc gcggctttct
     9121 ccacctcgtc tgccgactac aatttctccg caaacctatc cgcaacgaac accggagcca
     9181 tagccgaagg aagcaataaa tattatacag atgatcgtgt ggcgggggtt attgcggcta
     9241 caaccaccga cgccctcaca caaggcgcaa caaacaaata ttattcaaca aatcttttgg
     9301 cggcggatct ggcggctacg acgacaacgg ctttggccga aggtgataat aaatatttca
     9361 caaacgacag ggtcgccgcg gtaatagcgg gaacgaccac gacagccata gcggaagggg
     9421 agaaactcta cttcacagac acaagagccg atgacagggc tatcagtgtt ctttccgcca
     9481 caacatctct tccgaatgta agcacattgt ccggacttgt aacttttggt tcaacgacag
     9541 ccacgacaac aggagaagga aaccttaaaa ttacgggcaa tgcggaattg ggcactgtga
     9601 catccggcgc ttggaatggc acggcgatag gggacgctta tcttacaaaa acaggtgatt
     9661 ggacgggaac atttgacgga caagaagggt cttactattt agacgcacgc aatttaacaa
     9721 atttcggttc accgttcttc tcgttcttct cggccacaac tacagatgct cttgcacaag
     9781 gttcaacgaa taaatattgg tcacagacac tttttgataa tgcgctctct gcaacgactt
     9841 cactgcctaa tatcacgacc ctcgcaagcc tctctctacc cgttacgcaa ttgtcgggaa
     9901 attatgacgc gaagaatctt gcaaattttg gaactccttt cttctccttc ttctccgcta
     9961 cgaccaccga cgctcttaca caaggctcta caaacaaata ctggtcgcaa acactatttg
    10021 acaacgcact tacagcaacg acgacgcttc caaaaataac cacgctctct tctctctccc
    10081 tacccgttac gcaattgtcg ggaaattatg acgcgaagaa tcttgcaaat tttggaactc
    10141 cgttctttac atactttaat ggaacaacga cagatgcctt agcgcagggt tctattaata
    10201 aatattattc aacggatctt ttggcggcgg atctggcggc tacgacgaca acagccttgg
    10261 ccgaaggtga taataaatat tttacaaacg acagggttgc cgcagtaata gcggggacga
    10321 ccacaacagc catagcggaa ggggagaaac tctacttcac gaacacaaga gccgacgaca
    10381 gggcgatagg tgttctttcc gccacaacat ctcttccgaa tgtgagcgca ttatcgggac
    10441 ttgcaacgtt cggttcggga acagctacaa ccaccgcaga gggacacctt aaggttacag
    10501 gcaatactga attaggaact gtgacagcgg gtatatggaa cggcaccgcc ataggggatg
    10561 cttaccttac aaagacagga gactggacgg gaacatttga cggacaagag ggctcttatt
    10621 atctaaatgc aaaaaatctc acaaacttcg gttcaccgtt cttctcgttc ttctccgcca
    10681 cgacgacaga tgcactcgcg gaaggttcga taaacaaata ctggtcacag acactatttg
    10741 ataatgcgct tacagcaaca acgacacttc caaagataac aacactcctt ggacttacga
    10801 acgcgacaac aacaaatctc accattacga gtaacgccta tttccctagt ggtatctgga
    10861 actcatcggg taacgtcggc atcgggacat caaatcccac acacgaccta gagacgtata
    10921 cgtctggtgg tgcagctatg tatctaggac acagcaatgc ttccggttct ttccctaagg
    10981 taagcgctat cgggctcggt tcaaatgcgg tctcaaatag ttacagcacc aatggaaata
    11041 caatgtcggt cgtgggcagc gcgcagattg ccgcgataca atcgcaatca tccggggctg
    11101 aaacagacct tgctctttac accgccacgg gcggaagcgt aacagagaga atgagaatta
    11161 acagcgcagg taatatcggt attgggacag tggcaccggc taacgtgaaa ttagaagtaa
    11221 gtggggccgc aaccgcagat actaatggta acggggtaag tttaacccag acattaaccg
    11281 acacgacagc atttgcgacc ggtgtgggtg gaggacttgg ttttgctgga tattatactg
    11341 ctaatccaga cagggctgtt tggtcagcgg taaagggttt caaagaaaat tccacaacag
    11401 ggaattatgc gggagcttta agtttttata caagagccaa cggaggttct ctaactgaaa
    11461 aattaagaat tgattctact ggcaatatcg gtattgggac gacaacgcca gcacaagccc
    11521 taacaataaa ctcaactgtt tctgggatag cacctaaact acaaatactt ggaggaacac
    11581 cgctatcggt aggaaacgta acagcggcag tcatattggg aatgaatgaa ggtgccgtcg
    11641 ctcaggatgg tgtagagctt caggctgttc gttccgcatg ggtaaatccg aatgcgaccg
    11701 acttcgcaat atctgtagcg ggttcagaaa gagtccgagt agataaaact ggcaacgtcg
    11761 gtatcggcac gggtgctccg ggcgccaaac ttaccatagc cgccggagcc ttccagaccg
    11821 atacagaaaa tataagattt aatcgaacag aagactcggc tagatacaac tcaatatata
    11881 gcaggtcaga cgcaaccgcg gccgctaaca atattgcctt taaggttcat aacgcggtag
    11941 acacgacatc ccagacggag gtaatgaggc ttcttggtaa cggaaatgtc ggtatcggca
    12001 cgacgaatcc agatgcaaag ttggatgtag ctggtctaat caaaacagag ggagctttga
    12061 acagttttag aaactattat gacgctacac atttttcaga aataacagct aagagtaacg
    12121 gagatttaca gatgtacggt tttaacggag ctacttatca gaacatactt ctagcaatgg
    12181 acggaaacac acaggctggc aacgtcggta tagggacgac gaatccaacc cataaactgg
    12241 aaacttatag tgacaatgga gtttcggcat acttaggaca ttcaaatact accgggtcat
    12301 ttccaaaagt tagtgcgttt ggtcttggct ccgatgctgt cactaaggga tattcaaata
    12361 acggcgggac cttaacagtg acaggaagtg cccaaatcgc agcaattcaa tcgcaatcat
    12421 caggtgcaga aaccgcactg gccttttata caacaactgg cgggagcgta gccgaaaaaa
    12481 tgaggatagg gaatacgggc aatatcggta tcgggacgac gaatcctcag ggccgtttac
    12541 atatcggctc tactacttat tctacattga gcccttctta tcagtttaat atgtttggtc
    12601 cgcttaatat tatgcaaaga gatgcggcaa ccgactctta tgttactaac aatgcattct
    12661 ttaatagcgg taatcaatgg cagcgcgttt tggcaggcgg ggcaggaaga attggttggg
    12721 attctgatgc ggttggagca atggatttca aagttgcccc gacaggcacg gctgggtcta
    12781 ccttctcttg agtagacgcc atgaccattt taggaactgg taacgttggt atcgggacaa
    12841 cgacaccgga caccaagttg tctgttaacg gaaccacaaa atttggaaat ggagtctggc
    12901 cagaactagc catcggaatg tccggaacca gaatgggagt tcttaacgat acagagaatc
    12961 cttccctgat aatgagcaat atgtcaaata ccgccgcggc aaataacggt tctgaaatat
    13021 atttgggcgc gagaaaaaca acgggtcttg cagattgggt cggtggaatg atttcggggt
    13081 ataaagaatt tgcttccgga gtggaaggcg gctatctggc tttcaaaaca gtaaattccg
    13141 cggggacgct aacggaaaga atgagaataa atacttccgg tagtgtggct atcgggacaa
    13201 caacaccaca agccgaacta cacatagaag gtggctcgtt caataccaga ttgttggtga
    13261 atcaatccgg ggcggatggc gggatgatga atgcatttat gggtgtccag gtcagtgctt
    13321 tttctgacac cagtggtgtt ataaggttta aggacaaggg agccaacgtg gccgcaaatg
    13381 aattcttcgt tatcgagaat tcaaatgcgt caaacgatgc agcggccgga ttgctaaatg
    13441 ttaaaaataa tgccggttct attttaagcg taaaacacgg cggaacgata tttgcctcag
    13501 cttcactcgg agcggaatca ggcggcgaca actatctatg catagatcct actacgcacg
    13561 agattacaaa cggtggagca gattgcggtg cgtcgtctga aagatacaaa gagaacatca
    13621 aagacctcgg atatggccta aaagatgttc taaaaatgag acctgtatct tataaattca
    13681 gagagacggg gagagattcc ataggattta tcgcggaaga ggttattaat ataattcccg
    13741 agatagtaga gaccaaagac ggattaccgg aaagcattga ttacaataag tt
//
LOCUS       DAOTEX010000015        13602 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3118
            NODE_3135_length_13602_cov_4.463497, whole genome shotgun sequence.
ACCESSION   DAOTEX010000015 DAOTEX010000000
VERSION     DAOTEX010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868142
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 13602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 883
            CDSs (total)                      :: 833
            Genes (coding)                    :: 829
            CDSs (with protein)               :: 829
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13602
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3135_length_13602_cov_4.463497"
                     /isolate="AMR_MDS_3118"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(86..733)
                     /locus_tag="P5056_01510"
     CDS             complement(86..733)
                     /locus_tag="P5056_01510"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRY62427.1"
                     /translation="MHEIQEQILKQLTLSDKSRYSAIKPPKIGGNNFAYHLKEVIKDG
                     LVKKEGLFYKLTTKGERLADTISLKTFAKRAQPKIVTMLCLKNKADEILLFKRDKQPF
                     IGKCSLPYGKIHLGERVEEAAKREAEEKTGINIGKLKHAGDVYLAIYKENDLVSHMLC
                     HVFTGNTGSAKPKENCRFEKISTIKSSNLVPGTLDIIRLTENNKEKFFKEIFVEE"
     gene            962..1831
                     /locus_tag="P5056_01515"
     CDS             962..1831
                     /locus_tag="P5056_01515"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRY62428.1"
                     /translation="MACIEKIYSVSALVGSSACNGNCAFCAGKYLRPQASDNPIYWKN
                     LESAIKLSARYGGWSLSLTSSGEPTCDPEAVTKALEVYDKCAKQGAYFPNVNLFTNGI
                     LFGDSAFCDKWLPLWKNLGLTNVAISIHSVDRKKQAEAYGLESYPDFNDIFNNIRRHG
                     VGVRCTLLLRKGEVDNAVKYEEAVSTLVKFFGVDNITSWPVGNPDGTKNSFTPSWFGL
                     ASIRYWLWKNTKLCHGHAWGGGVFDWKGNILRLTDYVTRHDPAKDFVRQLVVFQDGTV
                     AYSWIREGALCMK"
     gene            1819..2652
                     /locus_tag="P5056_01520"
     CDS             1819..2652
                     /locus_tag="P5056_01520"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRY62429.1"
                     /translation="MHEIEITGNVKSVSVFIGTGKCNGNCKHCAGLIHRKYAPDEDGV
                     INKNLSDGDIYKILESCHYKGARSLSISSSGEPTLSPKSVTRLLELVDFLKSDQLKYN
                     KINLYSNGIRIGEDEDFVKTYLSDWSLWGLSSVYITVHSADEKENAKIYGVPSYPSLR
                     TIVSRIHNVGISARANVVLSKNSVGSLESFMNLVSQLKDIGFDNVSAWPVRNEDDKVD
                     ELLAPSKEEMEKICRFAVENSTNSFVIRVLGEEHHELYKTGQKLTLFPNGKLLNTWCN
                     N"
     gene            3710..3931
                     /locus_tag="P5056_01525"
     CDS             3710..3931
                     /locus_tag="P5056_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62430.1"
                     /translation="MDHPGMQAANERRHRQWKLEDKVRNIPLSEFTASELSALVRLTC
                     PPRDITSGEMDLIEKALKRINGNKKGGSR"
     gene            3928..4260
                     /locus_tag="P5056_01530"
     CDS             3928..4260
                     /locus_tag="P5056_01530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62431.1"
                     /translation="MREEDKHVDKCEICDTFPTYRNRGLSHDYWCWACECKIFADTAE
                     EWNKINSHKLERRSACHEVRQLLDQLFKSHLSIVKFDINKCAIERGNLEEIQDFAKTL
                     RSLIEKSR"
     gene            complement(4318..4914)
                     /gene="clpP"
                     /locus_tag="P5056_01535"
     CDS             complement(4318..4914)
                     /gene="clpP"
                     /locus_tag="P5056_01535"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007782171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HRY62432.1"
                     /translation="MLIPTVIEKTHAGERAYDIYSRLLKDRIIFLAGPIDDDLANIII
                     AQLLFLESEDPNKDISLYINSPGGSVTSTMAILDTMNNIKPDVATICVGLAASGAAVA
                     LSAGKKGKRFALPNSEVMIHQVMGGAEGQASDIEISAKHILRMKENLNKILSKNTGKP
                     VAQIEKDTDRDNFMTAEEAKKYGLIDEILKPKTNSRAN"
     gene            complement(5071..6153)
                     /locus_tag="P5056_01540"
     CDS             complement(5071..6153)
                     /locus_tag="P5056_01540"
                     /inference="COORDINATES: protein motif:HMM:NF017508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HRY62433.1"
                     /translation="MIEGTIKKLENSEIEIIGSISAEKFESYKQKAIKDIGEHINLDG
                     FRKGHIPEDVLIKEVGEMTILEEMAEAALRDAYPKFIIENKIEAIGRPNISITKIAKN
                     NPLEFKIVTAVMPVIGDTNHKNVAKSVWGELSKEKLDVTDKEVEDVVMEIRKARAKKD
                     DGHKCEDEHCKENHDKEKNPPTKAEEAPLPELNDEFVKSIGNFTGVEDFKEKIKKNIG
                     IEKESAQKEKARTQILDKIIEKTKFTIPEVLIKGETNQILHELRGKIEQLGLDFEEYL
                     KQTKKTEEDVRKEAKEPAEKRVKYKLILKAIAKEENIQIPQEEVMKEVENLLKYYEGA
                     DMESAKSYVEDTMINEKVFELLESNK"
     gene            complement(6495..6746)
                     /locus_tag="P5056_01545"
     CDS             complement(6495..6746)
                     /locus_tag="P5056_01545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62434.1"
                     /translation="MKRYNKGNAGPLVASVIIVLVLLVSSYYALKERPLKPEAPIDSD
                     IKALETQSTSTEMTDIEADLNATNLNDLGLDLETAEAEL"
     gene            complement(6778..7455)
                     /locus_tag="P5056_01550"
     CDS             complement(6778..7455)
                     /locus_tag="P5056_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62435.1"
                     /translation="MKKIFVVLAVALLAPIAVVRAETVDGTTASPVTAKPAVVKLEER
                     VKKVEQKAEDKKAKIADKLEAKQDKIDGKASTTKERIEKKIEVKKEANKVKALELAKR
                     AERKITAAIQRIELLANRVTDRLNILADKGVVKADTKKEVEAKLAEAKTLIADAKDSA
                     SKISVSADAAIASSSPKEALKDTKEAAKKAEDAVKEAHAKVVEAVTLIKASGEKNAEN
                     ATTTPNK"
     gene            7625..9391
                     /locus_tag="P5056_01555"
     CDS             7625..9391
                     /locus_tag="P5056_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HRY62436.1"
                     /translation="MFIEPKVLKDFILDSGLVSKKELENAELEAEKKGEDLGEYLVSS
                     GKITEADYNLIKGHILGIPVVNLKDQKIDFAVLSAIPEPIARKHNIVAFRKTEKELEV
                     AMLDPNDLRAIEFIKKRVGLKILPRLTDKESIKSALLNYQKSLKAEFGDIISSEAKVI
                     GATYVSEGEEGEPDAGELKKLAEDVPIVRIVDSLLSHAFTQQASDIHMEPFEDRLLVR
                     YRIDGILHDAMTLPPEVASGITARIKVLSNLRLDEKRLPQDGRFKVDSFGGAESKVSF
                     RVSILPTSFGEKIVIRLLPESNKGFTLEALGFHGEGLERVHNAMKQTTGMILVTGPTG
                     SGKTTTLYTIIDILNTPEVNISTIEDPVEYQMARVNQTQVKPEIGLTFASGLRALLRQ
                     DPNVIMVGEIRDKETSGLAINAALTGHLVLSTLHTNSAAGAIPRFKDMGGEPFLLVST
                     LNLVVAQRLVRKIAGNAEKYFLTATEIKALEKKADMNKVISVLKEENLVPKEATWEKI
                     SFVKPKQSEEFPSGYAGRVAIHEILMVTPAIRNMIIKDTTADEIEDQARKEGMLTLFE
                     DGIYKAAQGLTSVEEVLRVANE"
     gene            9384..10601
                     /locus_tag="P5056_01560"
     CDS             9384..10601
                     /locus_tag="P5056_01560"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HRY62437.1"
                     /translation="MSKFEFKARKASGEIVEGIREGSDRFQLSRELVSEGFIPVSIKP
                     AQTKVKYDFAFFNELFVRIKLHEKIMFARNLSSMLSAGLSLSRALSVLHKQTDNVKLK
                     RILKSILEDVESGRSLSQATSKFPNVFSSLFVAMIAAGEESGSLPASLKIVSEQLDKV
                     YVIRKKVSGAMVYPAIIFVAMIIIGALMLVYVVPNLTKSFAEFGAKLPYSTQLVVNSS
                     NFLLNHYLAAIMLLALLSSLIYYGFNSKRGQRTVDYIFLRLPVVSSVVKQINSASAAR
                     TISSLVSSGVTIVKSLEITTEVVQNYYYKEALTHAAEKIQKGGSMSDFFKEEEKLFPI
                     LMGEMAEVGEETGNLPAMLMNVAVFYEGEVDALTKDMSTIIEPVLMLFIGLAVGFFAI
                     SMVQPIYALSGNV"
     gene            10588..12462
                     /locus_tag="P5056_01565"
     CDS             10588..12462
                     /locus_tag="P5056_01565"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRY62438.1"
                     /translation="METSKEDFIFGTTSLKAPCGGVKQNMKRNKQSGFTLIEALVGIF
                     VFAVVVSSILEAYYVLIGSARNSRLKVSATLLANEQIEIIRNLPYSSVGVVGGIPNGI
                     LDPEKTIIKDAATFIIKTTIRNKDDSFDGTLGGNPNDVAPADYKLAEVEVSCGTCKNF
                     PPIILTTNVAPKSLETSSNNGALFVNVFDALGQPVPGAVVNIVNSYVTPQVNMSDVTG
                     ISGALQLVDVISSNQKYKISVSKDGYSSDMTYTPRLAANPNPTKPDATVASKQVTQIS
                     FSIDKLSALNVSTVTSTCTSTPNINFRLVGSKLIGTNPDVYKFSADYTTGADATKTID
                     NLEWDTYSVSIPNTLTSTHAMAGTLSMVPVSLLPDTTQDLKIIVAPKNLNGLLISLKD
                     SGTGLSVSGAEVTLRKNGTSVVLTTGRGSKAQSDWQGGAGQADLYDETKYYSSSNVNT
                     ASSEGEIVLSSTLGEYETDGTLESSTFDTGSEGNFYNLNWLPIDEPTASGPDSVKFQI
                     ATNNDKSTWNYVGPDGTGNTYFTSSGEALGASHEGRRYIRYKVFLHTDDVASTPNISN
                     VSFGFTSDCVPSGQVFFDGLSLGDYELDVVKEGYNSILALPVSVGGAWQEKVVLMSLK
                     "
     gene            12476..13096
                     /locus_tag="P5056_01570"
     CDS             12476..13096
                     /locus_tag="P5056_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62439.1"
                     /translation="MNQELRIKNTKKGLTLIELLFGMAFIAFMGLAIANFQNSMFSSS
                     EYLQRSLMAEGDARTVLRQLISELRAMNYSSVGSYPISSALENSITFYSDTNGDGNPE
                     RLRYFLDGDLFKVGVVYPSGAPLTYDINSERIKTLVRKVDPTASIFTYHGTEYTGENG
                     ALVFPVDIPLIRLVKVNIPIIIENQGTTTPYSISSQVSLRNLKENL"
     gene            13103..>13602
                     /locus_tag="P5056_01575"
     CDS             13103..>13602
                     /locus_tag="P5056_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62440.1"
                     /translation="MNHVFNKNRAKRYLTGGAILVQLLVLAGLSSILISSLVAWSASS
                     LRSSRREFNRELSFQIAEAGIEYYRWHLTSTSTDYTDGTTLSGPYIHDFKDKNGNTIG
                     SFSLQITPPLIGSTRVKITSTGTLSSDPDSARTIEVEVAKPSLGKFSVITESDIRFGE
                     GTEVFGP"
BASE COUNT         3603 a         2712 c         3053 g         4234 t
ORIGIN      
        1 gcgccaatac agtttttaac atcaaagtgt ttccaagcta ttgattagaa aacggaccta
       61 gcaaaaagac tcttttttgg ggtctttatt cctctacgaa gatctctttg aagaatttct
      121 ccttattatt ttctgtaagt ctgattatat caagcgttcc gggcacaaga ttagacgatt
      181 ttatggtcga aatcttctca aaacggcaat tttctttggg tttcgccgaa cctgtgttgc
      241 ctgtaaaaac gtgacaaagc atgtgcgata ccaagtcgtt ttctttatat attgctaaat
      301 atacatcacc tgcatgtttt aacttaccaa tatttattcc cgttttttcc tccgcctctc
      361 tttttgcggc ttcttccact cgttccccta ggtgtatttt tccgtagggg agggaacatt
      421 ttcctatgaa aggttgtttg tctcttttga ataacaatat ttcatctgct ttgtttttta
      481 ggcaaagcat cgtgactatt tttggttgcg ctctttttgc gaaggtcttt aggcttattg
      541 tgtcggccag ccgttctcct tttgtggtga gtttataaaa aagcccctct tttttcacga
      601 gaccatcctt gatgacctct tttaggtgat atgcaaagtt gttaccgcca atcttgggcg
      661 gttttatggc agaatatctt gatttatctg aaagcgtgag ctgtttcaat atttgctctt
      721 gaatttcgtg catatgatag gtttatcact caacatattt tgagtcaaac acattagact
      781 agtttgtggg tttatggttg ataaaatatt ttaaagtaaa gttcattgaa aacttgcccg
      841 caaaaatttc cataggaact attttggttt tgggtcacgt tgagaaacca taaagttttt
      901 cagcattata tggtcactga gcgttaagaa atttcggcgg gtttaaaaag gaggggtttt
      961 tgtggcttgt atcgagaaga tctacagcgt ctccgctctc gttggctcta gcgcgtgtaa
     1021 tggtaattgt gctttttgcg cggggaagta tctccgtcct caggcctctg ataatcctat
     1081 ttattggaaa aatttggaat cagctatcaa gctttcggct cgttatggcg gttggtcact
     1141 ttccttaaca agcagtggcg agcctacttg cgatccggaa gcggtcacta aggcgctcga
     1201 ggtctatgat aagtgcgcta aacagggagc ttatttccct aacgttaatc tttttacaaa
     1261 cggaattctg ttcggcgata gcgctttctg tgacaagtgg cttccattat ggaaaaatct
     1321 cggacttacg aacgttgcga tctcaattca ctcggtcgac agaaagaagc aggccgaagc
     1381 ctacggtctt gagagttatc ctgacttcaa tgatattttc aataatattc gccgtcacgg
     1441 tgtcggcgtg cgttgcaccc tgcttctccg taagggcgaa gttgacaacg cggtgaaata
     1501 cgaggaagct gtcagcactc tcgtgaaatt ttttggtgtc gacaatatca cgtcttggcc
     1561 ggttggtaat cctgacggca ctaaaaatag tttcactccg tcgtggtttg gactcgcgtc
     1621 catccgttac tggctctgga aaaacaccaa gctgtgtcac gggcatgctt ggggcggtgg
     1681 tgtcttcgat tggaagggga atattcttcg tttgaccgat tatgtgacga ggcacgatcc
     1741 cgctaaggat tttgtccgtc aattggtcgt ctttcaggat ggaaccgtcg cttattcttg
     1801 gattcgtgag ggtgcgttat gcatgaaata gaaatcaccg gaaacgtcaa aagtgtttcg
     1861 gtgtttatcg gcaccggaaa atgcaatgga aattgcaagc attgtgccgg attgatccac
     1921 agaaaatatg ctcccgatga agacggagtt attaacaaaa atctaagcga tggtgacatt
     1981 tataagatct tggaatcttg ccattacaaa ggcgctcgct ctttgtcgat aagtagtagc
     2041 ggtgaaccca cgctttcgcc aaagtctgtg acccgtcttc ttgagttggt tgattttctc
     2101 aaatcagatc agcttaagta caacaagatc aatctttatt caaacggaat aaggatcggc
     2161 gaggatgaag attttgttaa aacgtatctt tcggattggt ctttgtgggg gctttcgtcg
     2221 gtctatataa ctgtgcatag tgcggacgaa aaagagaacg cgaaaatata tggagtccct
     2281 agctatccca gcctgaggac tatcgtgtcg aggatccata acgtcggtat ctcggcccgg
     2341 gccaacgttg ttttgagtaa aaattccgtt ggctctctcg agagcttcat gaatctcgtc
     2401 agccagctta aagatatcgg attcgacaat gtgtccgctt ggccggtgag aaatgaagac
     2461 gataaggttg atgaattgct cgccccctcc aaagaagaaa tggaaaaaat ttgtcgcttt
     2521 gccgttgaaa atagcacgaa tagtttcgtt atccgtgtcc tcggcgagga gcatcacgag
     2581 ttgtataaga caggacaaaa gttgactttg ttcccaaacg gaaaactttt gaatacctgg
     2641 tgtaacaatt gaatatttat aaccgctctt tcgggggcgg ttttttaatt gctcacctac
     2701 gtttgagtca attattttag actagtttga atattttatt tttattaaat ttagagtctc
     2761 actagttcat tgaaaacttg cccgcaaaaa tttccgtagg aactattttg gttttgggtc
     2821 atgttgaaaa accataaagt ttttcagcac gatatggtca ctgagcgtta agaaatttcg
     2881 gcgggttaaa taaattaagg aggggttcgg atgggatatt atgactcttg ctacgaggcc
     2941 gacgaggaaa gggaaagaga aaaatgggta aataagtgcc gttcttgcgg agaacatcct
     3001 aaatttacgg aaaccgaatg aaccaaattc tattcttgtc gatgtggttt tacttctaaa
     3061 aaccgcgagg actggaacaa gaccattcgg atcaagtctg aacgctttga atttcgcagg
     3121 aagcttgaaa aactcatcag tgaattttcc ggtagtgctg gccctgcaat ttctctaaat
     3181 ttttcttgga ttgatattac cgatgagcag tttaatctca tgaaaaaact agagaacttg
     3241 cttctgtcga ttacaaagaa gaaggagggg caatagaaat gtctagaacc aattgcgaca
     3301 tttgttccaa gaaaggatat tactattgta aatgcatact tcgcagggcc acacaatatg
     3361 ctattaagtc ttatgaatat tcccacggta ttaaactttc ttcgattttg aaagacaaga
     3421 aatgtcctac ttgcggaaaa gagttcgata aatgccccac atgcggtaaa tagccctcct
     3481 ttaacaaaac cgccccggtt tcccgaggcg gtttaatttg gacgacgatg ttgcaactga
     3541 aggaattttg tgtagaatca ccgttgtaat tgttttttga caaaattgag aggaaattag
     3601 aaaagaaagg agattttctg tgtctgaaga gcggaaagaa tgtctagatt gcaaggatct
     3661 ctttttcatt ttatgtttat ggcctcaaag aaagaaaaga ggggatagta tggaccatcc
     3721 aggaatgcaa gctgccaatg agagaagaca tcgtcagtgg aagctcgaag ataaggtaag
     3781 aaatattccg ctctccgagt ttaccgcttc ggagctcagc gcccttgttc ggctcacttg
     3841 tcctccgcgt gacattacta gcggtgaaat ggatttgatt gaaaaagccc ttaagcggat
     3901 taatggcaac aagaaaggtg gctcccgatg agagaagagg ataagcacgt agataagtgt
     3961 gagatctgtg acactttccc gacatacaga aaccgtggac tgtctcacga ctactggtgt
     4021 tgggcgtgtg aatgcaaaat tttcgctgat acagccgaag agtggaacaa gataaattct
     4081 cataaactcg agaggcggag tgcttgccat gaggtgcgtc agcttctgga ccagctgttt
     4141 aaatctcatt tgagtattgt taagttcgat atcaataaat gtgccattga acgaggaaac
     4201 ctggaagaaa tccaagattt cgcaaaaaca ctcaggtcgt tgatcgaaaa gagtcgatga
     4261 ttttctcctt aaaaatcaaa accgccccga ttttctcgga gcggttttat ctttgcttta
     4321 gttagcccta ctgtttgtct ttggttttaa tatctcgtcg ataagaccgt atttcttggc
     4381 ttcttcggcc gtcataaagt tatctcggtc tgtgtctttc tctatttgag ccacaggctt
     4441 tcctgtgttc tttgaaagaa ttttgttaag gttctccttc attctaagga tgtgcttggc
     4501 tgagatctca atatctgaag cctgaccttc ggcgccaccc ataacttggt ggatcattac
     4561 ctcagagttt ggaagagcaa atctttttcc tttctttccg gcagacaagg ctaccgcggc
     4621 tcctgaagcg gcgagaccga cacagatagt ggcgacatcc ggcttgatgt tattcattgt
     4681 gtctaatatt gccattgttg acgtcactga gcctcccggt gaatttatgt acaagctaat
     4741 atccttattt gggtcttctg actcaaggaa gaggagctga gcgattatga tattcgcaag
     4801 atcgtcatct atcggtcccg ccaaaaagat tattctgtct ttgagaaggc gagaataaat
     4861 atcatatgct ctctctccgg cgtgtgtttt ttctattact gttggtataa gcattttaat
     4921 tatgaattag caattaataa ttacgaattg agttgtttgt tttgctccat tcccgtgaaa
     4981 acgggcatcc atcaatatcc tctggatctc cgcctccgcg gggatgacat ttgaagatgt
     5041 tgccggttat attcttctac tttctatatt ttacttgttg ctctctaaca actcaaaaac
     5101 cttttcgttt atcattgtat cctctacata tgatttggct gattccatat ctgccccttc
     5161 gtaatatttt agaagatttt caacttcttt cataacctcc tcctgcggaa tctggatatt
     5221 ttcctccttt gcgatggctt tcaaaataag cttatattta actctttttt cagccggctc
     5281 cttggcctct tttcttacgt cttcttcggt tttctttgtc tgtttcagat attcttcaaa
     5341 atctagtccg agttgttcta ttttgcctct taattcgtga agaatctgat ttgtctcacc
     5401 ttttattagg acttcaggaa tagtaaattt tgttttttcg attattttgt cgagtatttg
     5461 agttctggct ttttcttttt gagcgctctc tttttcaatt cctatattct tttttatttt
     5521 ttctttgaaa tcttctacgc cggtgaaatt accgattgat tttacaaatt cgtcgttaag
     5581 ttccggcaga ggagcctctt ctgccttcgt aggcgggttc ttttctttat catgattttc
     5641 tttgcagtgc tcgtcttcgc atttgtgacc gtcgtctttt ttcgctctag cttttcttat
     5701 ctccatgacc acgtcttcaa cctccttatc tgtgacgtcc aatttttctt ttgatagctc
     5761 accccaaaca gattttgcga cgttcttgtg attcgtgtct cctataacag gcattaccgc
     5821 tgttactatt ttgaactcaa gggggttatt cttcgcaatc tttgtaatag agatatttgg
     5881 tctgccgatt gcttctattt tgttttctat aataaatttt ggataagcat ctcttagggc
     5941 ggcttccgcc atctcctcaa gaattgtcat ttctccaact tctttaatta gaacatcctc
     6001 gggaatgtgt ccttttctga agccgtctaa attgatatgt tcgccgatat cttttatggc
     6061 tttctgctta taagattcga atttttcagc gctaatagag ccgatgattt ctatctctga
     6121 attctccagt ttttttatgg ttccttctat cattttgatt gtttttccgc ctttttatgt
     6181 taatcgcgga ctattcttaa tatcatatat ttggtatcta agcaagccgg cattatgttt
     6241 ccaagctatt gaaccataac gcggggttga ctttttgttt ttgcgatggc aggttgtagt
     6301 aaatagttcc ttagtttcaa attaaaaaag ggggtattga cttggacgac gagtatttcg
     6361 aagatcctga cgagtgggac gacatcgacg atccggacga agagccctga accattgccc
     6421 ggtaaaaatt tatttatcgg cagacaaaca aaaacacccg aagtctcggg tgtttttcat
     6481 ttttattaga ttttttagag ttcggcttct gcggtttcta aatctaatcc caaatcatta
     6541 aggttcgtgg catttaggtc cgcttcaatg tctgtcatct ccgtggaagt gctctgggtt
     6601 tctaatgcct ttatgtcgct atcgataggg gcttccggct taagaggcct ctccttaaga
     6661 gcataatagg agcttaccaa gagaaccaag acaatgatca cagaggcgac gagtgggccg
     6721 gcatttcctt tattatatct tttcattttt taaatattta tttgatttta taaagcctta
     6781 tttgtttgga gttgttgtcg cgttttcggc gttcttttct ccggaagcct ttatgagagt
     6841 gacggcctca actacttttg catgggcttc ttttaccgca tcctcagcct tttttgcggc
     6901 ctccttcgtg tctttcaggg cctcttttgg cgaagagctg gcaatcgcag cgtctgcaga
     6961 gactgaaatc ttagatgctg agtctttcgc atctgcgata agtgttttgg cttcagccaa
     7021 tttggcttct acttcctttt ttgtatccgc ttttacaact cccttgtctg cgagaatgtt
     7081 caatctgtct gtaactctgt tcgcaaggag ctctattctc tgtatggcgg ctgttatctt
     7141 tctctcggct cttttggcga gctcaagggc ttttacttta ttggcttctt ttttgacctc
     7201 aatttttttc tcaattcttt cttttgttgt agaggctttc ccgtcgattt tgtcttgctt
     7261 ggcctcaagc ttgtcggcaa tttttgcctt tttgtcttcg gccttctgct ctactttttt
     7321 aactctttct tcaagcttta caacggcagg ttttgccgta acaggggagg ctgttgtgcc
     7381 atctactgtt tcagctctta cgactgcgat cggtgcgaga agcgcgaccg ccaatacgac
     7441 gaaaatcttt ttcatttttt aaaaagatta tttttataat tttttttgac tttttctata
     7501 atactaccct tttcgctaaa gtcaaaatta aaaaattacc cttatatatc aaagtatttt
     7561 acctgtgtat ttccacagct tactcatgat gatgtgtgat ataatattta gataatccca
     7621 aattatgttt attgaaccaa aagtattaaa agattttatt ttggattccg gtttggtgtc
     7681 caaaaaagag ctggaaaatg ccgaattgga ggcggagaaa aaaggagaag acctgggaga
     7741 gtatttggta tctagcggga aaataacaga agcggattac aatctcatta aggggcatat
     7801 tttgggcata cccgttgtta acttaaaaga tcaaaagatc gattttgcgg ttctgtctgc
     7861 aattccggag cctattgcca gaaaacacaa tattgttgcc ttcagaaaaa ccgaaaaaga
     7921 gctcgaagtc gccatgcttg atccgaatga ccttcgcgct atcgagttca taaagaagag
     7981 ggttggtctc aaaatcttac caagacttac agataaagaa tccattaagt ccgcactttt
     8041 gaactatcaa aagagcctaa aggcggagtt tggggatatt ataagtagcg aggccaaggt
     8101 tataggcgcg acatacgtta gcgaggggga ggagggcgag cctgacgccg gagaattgaa
     8161 gaaattggcc gaggatgttc ctattgttag gattgttgac tcccttcttt cgcacgcttt
     8221 tacacaacag gcttcagata tacatatgga gccgtttgag gatcgacttc tggtcagata
     8281 tagaatagac ggtattttgc acgatgcgat gactcttcct ccggaagtgg cttccggtat
     8341 tacggccagg attaaggttc tttccaacct aaggcttgat gaaaagaggc ttcctcaaga
     8401 tggtagattc aaggttgatt cattcggagg agctgagtca aaagtatctt ttcgtgtttc
     8461 tattttgccg acatcgttcg gcgagaagat agttattcgt ttacttccgg aaagcaataa
     8521 gggcttcact cttgaggcct taggtttcca cggagagggg cttgagaggg ttcataacgc
     8581 catgaagcag actacgggaa tgatactcgt gacaggtccg accggctccg gaaagacaac
     8641 gacactttat acgattatag atattttaaa tacacctgag gtcaatatct ccaccataga
     8701 agacccggtt gaatatcaaa tggcgagagt aaatcagact caagtaaaac cggaaatcgg
     8761 tctgacgttt gcatcgggac ttcgcgctct tttgcgacaa gatccgaatg tgataatggt
     8821 cggagagata agagacaagg agacttcggg tcttgccata aacgccgcgc ttaccggtca
     8881 ccttgttctt tctacacttc acacaaattc cgcggccgga gccataccgc gatttaagga
     8941 tatgggaggg gagccgtttc ttcttgtttc taccctgaat ttagtagtcg cgcagaggct
     9001 tgtcagaaag attgcgggga atgctgaaaa atattttttg accgctacgg aaataaaggc
     9061 gctggagaaa aaagcggaca tgaataaagt tatctctgtg cttaaagaag aaaatcttgt
     9121 tccaaaagaa gctacttggg agaaaataag ttttgtaaaa ccaaaacagt cggaagaatt
     9181 tccttccgga tatgcgggca gagtggcaat acacgaaata ctcatggtga cgccggctat
     9241 aagaaatatg attataaagg atacgacggc ggatgaaata gaggatcagg cgagaaaaga
     9301 aggcatgctt acgcttttcg aagacggtat ttataaggcc gcgcaaggtt tgactagcgt
     9361 tgaggaagtg ctgagagtgg caaatgagta aatttgaatt taaggcgaga aaagccagtg
     9421 gggagatcgt tgaagggatc agagagggct ctgaccgttt tcagctttct cgcgaattag
     9481 tttcggaagg atttattccc gtttcaatca agcccgcaca gacaaaagtt aaatatgatt
     9541 ttgctttttt caatgaactg tttgtgcgta taaaactgca cgagaagata atgtttgcca
     9601 ggaatttgag ctcgatgctt tccgcaggat tatctttgtc tagggcgctt tctgttctac
     9661 acaagcagac ggataatgtt aaattaaaaa gaattctaaa atctattctt gaagacgttg
     9721 agtcgggaag aagtcttagc caggccacgt caaaatttcc aaacgttttt tcttctttgt
     9781 tcgtcgcgat gatcgccgcg ggagaggagt cgggaagttt accggcttcc ctgaaaatag
     9841 taagcgaaca gttagataaa gtttatgtga taagaaaaaa ggtttcgggc gccatggtat
     9901 atccggcgat aatttttgtc gcaatgataa taatcggtgc gctcatgctt gtttatgtgg
     9961 ttcctaattt gaccaaaagt tttgccgaat ttggagcgaa gctcccttat tccacgcaac
    10021 ttgtagtaaa cagcagtaat tttctcctga atcattattt ggcggcgata atgttgcttg
    10081 ccttattgtc ctcgctaatc tattacggat tcaattcaaa gagggggcag aggacggtag
    10141 attatatttt tttaaggctc cccgttgttt cttctgttgt taaacaaata aattccgcct
    10201 ccgccgcgag aactatttcg tccctggtgt cttccggagt aacaattgtt aaatcgcttg
    10261 agataactac tgaagttgtc caaaattatt attataaaga agcgctgacg catgccgctg
    10321 aaaagataca gaagggcggt tcgatgtcgg attttttcaa agaggaagag aaacttttcc
    10381 ctatattgat gggtgaaatg gcggaagtag gagaggagac gggaaacctc cccgcgatgc
    10441 ttatgaacgt tgcggtattt tacgagggag aggtagatgc tcttacaaaa gatatgtcta
    10501 ctatcataga acctgttttg atgcttttca tcggtctggc cgtaggattc tttgctattt
    10561 caatggtaca gccgatctat gcgttgagtg gaaacgtcta aagaggactt tatctttggc
    10621 acaacttcgt tgaaggctcc gtgtggtggt gttaagcaaa atatgaagag aaataaacaa
    10681 tcaggtttca cactaataga ggcccttgtg ggcatatttg tttttgccgt ggttgtcagc
    10741 agcattttgg aggcatatta cgtgctcatc ggctccgcca gaaattctcg gttgaaagtg
    10801 tcggcgacgc ttctggccaa tgaacagata gaaataatca ggaatttacc atatagcagt
    10861 gtgggggtgg tcggcggtat cccaaacggc attttagatc ccgagaaaac aataataaaa
    10921 gatgcggcga cttttattat aaaaacaacg ataagaaata aagacgactc ctttgacggg
    10981 actttgggcg gcaatccaaa cgatgtcgct cccgccgatt ataaacttgc cgaggttgag
    11041 gtgtcttgcg gaacatgcaa aaattttcct ccgattattt taactactaa tgtggcgccg
    11101 aagagccttg aaacctcatc taataacgga gctttgttcg tgaatgtttt tgacgctttg
    11161 ggacagcctg ttcccggtgc tgtggtcaat atcgtaaata gttatgtaac accacaagtt
    11221 aatatgagcg acgtgacggg catatcaggc gccttgcaac ttgtagacgt tatttcatct
    11281 aaccaaaaat ataaaatttc ggtatctaaa gacggttatt cttctgatat gacatatacg
    11341 cccagattag ccgctaatcc caatccgaca aagccggatg ccaccgtcgc ttcaaaacaa
    11401 gttactcaaa taagtttttc gatagacaaa ttaagcgcct tgaatgtgtc cacagttacc
    11461 tctacctgca cgtctacccc taatattaat ttccgtctgg tcggctcaaa actcataggg
    11521 accaatccgg atgtttataa atttagcgcc gattatacaa cgggcgcgga tgctacgaaa
    11581 acaatagaca atttagaatg ggacacctat tctgtctcta tcccgaatac gcttacaagc
    11641 acgcacgcaa tggccggcac cctttctatg gttccggttt ctctcttgcc ggacacaacg
    11701 caggacttaa aaataattgt tgcgcccaaa aatcttaacg gcctgctaat ttccttaaag
    11761 gattccggca ccggcttatc tgtctccggg gcggaggtga cccttagaaa aaatggaaca
    11821 tctgtggtct tgacgaccgg tcgcggttca aaagcacaaa gtgactggca gggcggcgca
    11881 gggcaggcgg atttgtatga cgaaacaaaa tattattctt cttcaaacgt aaacacagcc
    11941 tcttctgagg gggaaattgt gttatctagc acactcggtg aatatgagac cgacggcaca
    12001 ctcgagtctt ccacctttga tacgggatct gagggtaatt tttataattt aaattggttg
    12061 ccgatagacg agcctacagc ttccggtccc gacagcgtta agttccaaat agcgacgaac
    12121 aatgataaat ccacctggaa ttatgtagga cctgacggca cgggaaatac atattttact
    12181 tcttcgggtg aggcgctcgg tgcttctcac gaagggcgca ggtatataag atacaaagtt
    12241 tttcttcaca cagatgacgt agcttcgaca cctaatatct ctaatgtgtc cttcggattc
    12301 acttccgatt gcgttccttc cggacaggta ttttttgacg ggttgtcgct gggagattac
    12361 gagttagatg ttgttaagga gggctataat agcattctgg ctctgcctgt gtctgtcggc
    12421 ggtgcttggc aagaaaaagt tgtgttgatg tctctcaaat agtacaaaaa gaattatgaa
    12481 tcaagaatta agaattaaga atacaaaaaa ggggcttact ttgatcgagt tattgttcgg
    12541 catggcattt attgctttta tgggtctcgc tatcgcaaat tttcagaata gtatgttttc
    12601 ttcaagcgaa tatctgcaaa gaagcctaat ggcggagggt gatgctcgca ctgtgttgag
    12661 acagcttatt tccgagcttc gcgcgatgaa ttattcaagt gtcgggagtt atcccataag
    12721 ctcggctctg gaaaattcca taacttttta tagcgacacg aacggggacg gcaatccgga
    12781 gaggctcaga tattttttgg acggagatct ctttaaggtc ggggtcgttt acccgtccgg
    12841 cgctcctcta acatacgaca ttaactccga aagaataaag acactggtga ggaaagttga
    12901 tccaacagca agtattttta cttatcacgg gaccgaatat accggcgaga atggagccct
    12961 cgttttcccg gtagacatac cgctgattag gctcgttaag gtcaatattc caattattat
    13021 agaaaatcag ggaacgacta caccctacag tatctcgagc caggtttctt tgaggaatct
    13081 gaaggagaat ctataaccaa ttatgaatca tgtttttaac aaaaatagag cgaagcgtta
    13141 tttgaccggg ggagcaatat tagtccagct tttggtgctg gccggccttt cctctatcct
    13201 aatatcctct ttggtggcgt ggagcgcgag tagtttgagg tctagcagga gagaatttaa
    13261 cagagaactc tcgttccaaa tagccgaggc gggaatagaa tattatagat ggcacctgac
    13321 atctacctcg actgattata cggacggaac cacgcttagc ggtccatata ttcacgattt
    13381 taaggataag aacggaaata caataggtag tttctctttg cagattactc ctcccttaat
    13441 tggttcgact agagttaaaa taacatccac gggcacatta tcttccgacc cggactctgc
    13501 aaggacgata gaagttgagg tcgcaaaacc atctttaggt aagttttctg taattactga
    13561 aagtgacata aggtttggag agggaactga agttttcggc cc
//
LOCUS       DAOTEX010000016        12904 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3118
            NODE_3395_length_12904_cov_4.541365, whole genome shotgun sequence.
ACCESSION   DAOTEX010000016 DAOTEX010000000
VERSION     DAOTEX010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868142
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 12904)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12904)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 883
            CDSs (total)                      :: 833
            Genes (coding)                    :: 829
            CDSs (with protein)               :: 829
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12904
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3395_length_12904_cov_4.541365"
                     /isolate="AMR_MDS_3118"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..933
                     /locus_tag="P5056_01580"
     CDS             <1..933
                     /locus_tag="P5056_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HRY62441.1"
                     /translation="GKDDIKKIIAIENFGRPKILFLDEIHRFNKAQQDFLLPYVESGK
                     ITFIGATTENPSFEIIAPLLSRCRVFTLKEHGEKEMAKIIDRTKIKIGKEAKEWLINM
                     ANGDARSAITMLENTQRFYGKINIENLKQTLQDKFLRFDKKGEEHYNTTSAFIKSMRA
                     SNPDAALYYLARMVAAGEDPIFIARRMVIFASEDIGLAQPTALVVANAVFRAVETVGY
                     PECSINLAHGATYLALAAKNRDSYNGLQEAMKDTEKYGNLPIPLKIRNPVTKLMKNLE
                     YGKGYEKYTKEDLLPEKLKGKKYLKRREDEPRKK"
     gene            1112..1855
                     /locus_tag="P5056_01585"
     CDS             1112..1855
                     /locus_tag="P5056_01585"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HRY62442.1"
                     /translation="MNFKTFFKSEMVSAATVLAIGLFLSAMGAAYTFYMIRTFDNTLS
                     TTGSAKAKVTSDSVKWSASISRTVTEGNIKSGYAGLIEDLAIVKKFLTSKGIAESEIT
                     TTPVFTNEVYKYNPSGNAGPKEFTLIQNITINSTDVAKITEVAKNTQDIINRGVFFQN
                     QSPEYYYSKLADLRVSLLGDAVKDAKARAEMLAKSGGRSIGAMKAASSGVVQVLAPNS
                     IEVSDYGQYDTQSIEKEVMVTARASFVVR"
     gene            1987..2424
                     /locus_tag="P5056_01590"
     CDS             1987..2424
                     /locus_tag="P5056_01590"
                     /inference="COORDINATES: protein motif:HMM:NF012895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HRY62443.1"
                     /translation="MFKKLKVKRIDKSINLPKYQTGGSVAFDFMSRKDIEIKPKEIAY
                     VPLNVCVKIPKNHTLLMAARSSLHKKGLFMANDIGVFDEDFCGDGDEYHAALYNFSDR
                     PVKIEKGDRLTQGFLVPIAKVEWKEVDKMDTCDRGGFGSTGSK"
     gene            2652..2933
                     /locus_tag="P5056_01595"
     CDS             2652..2933
                     /locus_tag="P5056_01595"
                     /inference="COORDINATES: protein motif:HMM:NF039198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5698 domain-containing protein"
                     /protein_id="HRY62444.1"
                     /translation="MNELMLFIVGIVEMIIVTIWTESVTKARVLESGIITIINIFIWY
                     YVLDTIVNDIGNFNLVLVYALGCSTGTMLGTHYISKREAAKALREAKSA"
     gene            3144..3560
                     /locus_tag="P5056_01600"
     CDS             3144..3560
                     /locus_tag="P5056_01600"
                     /inference="COORDINATES: protein motif:HMM:NF022114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gmad2 immunoglobulin-like domain-containing
                     protein"
                     /protein_id="HRY62445.1"
                     /translation="MVVKKKLIIILFYFLIISVAIGWMIKNIQEQENLNVVVFSPKAN
                     EAISSPYKITGKARGTWFFEASFPVILRDKSGKELGIAVAQATSDWMTEDFVNFVAEI
                     KFEPAGLKEGELIFKKDNPSGLPENDAEIVLPVRFK"
     gene            complement(3682..4014)
                     /locus_tag="P5056_01605"
     CDS             complement(3682..4014)
                     /locus_tag="P5056_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62446.1"
                     /translation="MIKIVKPLDKKYIMAIGQTIQTARAIGPARMTTHTETMPMIIVI
                     ERRSIPNRRIMMSMIMKVKSKNPFRASPSLFHGEKNVFRRREMEKKLKVNQIKFIQFI
                     KRSFIARV"
     gene            4015..6405
                     /locus_tag="P5056_01610"
     CDS             4015..6405
                     /locus_tag="P5056_01610"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HRY62447.1"
                     /translation="MIKTNPNTVRRASFSGILGLEKIFPHKTRRKVYLFLRNITIVIG
                     LLYLALIFAGGNESVTLKTLGVFMVFLGVAMALFSFEAFFASYYGGNESKVLSGQIII
                     SSKENLLKSFARSPFGLKVILRTEIPIEIFSELFNKNEGENEFLDIAKGDSPGDILNA
                     KELALAIFDKDQDLKKLLASYGIDKKIFEDILDWAIVDLEKEYRRELWWWPEYLIKIP
                     GIGKDWNFGRLFYLVKYGKDLTPEATSPSIDFDIKEFGVEVKQLENILSKGREANAML
                     VGEPGVGVLDMVLEFVRKLNRGKIHPALEYKRVFLLNWNILTASNKDKNSLERELINT
                     LNEALGAGNIILVIDDLPGFMASADALGVNVQSILDPYLASLLQILATSDNGKFHKVI
                     EKNPDLMRRFDKINIEETDEAKTIEIIENTVRKIEKTKGLFFSYPAIKEIVTGANQYF
                     PGTVMPDGAIDLLFEIISSSLRTNDYISRNDVLNFIEMKTKIPLGKMEATEKEKLLHL
                     EPILHERIVGQEEAISVISNAMRRTRADIRNSKKPIGTFLFLGPTGVGKTETAKALAS
                     VFFGDERFMLRLDMTEYQKADSLQRLIGSFESGKPGVLSAMLRENPYGVLLLDEFEKA
                     EKDVQNLFLQILDEGFFSDMDGKKVSLRNVIIIATSNAASDYIFQLVKGGKDPVGLKD
                     DVVQKIVDNGIFKPELLNRFDASVIFHPLDKSELFQIAKLMLIKLKKRLAEKGIEFVV
                     TDELAMAVATEGANDIFGARPMNRYIQDKIEQFIAKKMIAGELAGGSKLEINPKEL"
     gene            complement(6697..7377)
                     /locus_tag="P5056_01615"
     CDS             complement(6697..7377)
                     /locus_tag="P5056_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62448.1"
                     /translation="MSTKYFKVEQGYVEKGRYKGLYKIQIKSAGKNVADFWGLSEEEA
                     HNNLNRDGNYKIVGLHRKGKCVFMARLKSQKENPKDYFDVPMRYKPLTKATVKFTKCG
                     HMGPEKAELNFHGRITQFDVGETNNVNGQKTVDDCECPRCYFNNTKRQVIRCCLCGIG
                     IEPGNGVILVDGRLHERFRKEFATMIGDKSISCLRFACCPGAESHPLGYWTGEGFELN
                     KHLAKRNK"
     gene            complement(7453..7524)
                     /locus_tag="P5056_01620"
     tRNA            complement(7453..7524)
                     /locus_tag="P5056_01620"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7489..7491),aa:Glu,seq:ctc)
     gene            complement(7646..8479)
                     /locus_tag="P5056_01625"
     CDS             complement(7646..8479)
                     /locus_tag="P5056_01625"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate mutase family protein"
                     /protein_id="HRY62449.1"
                     /translation="MSKLTANKTKMPKNLFLVRHGQSEGNFVRKRVKDTGDESYFSDE
                     FMALHESQYQLTKLGIEQAKKAGEWLRKNKFIMFDRMLVSNNIRAMQTAAYLEIDNAS
                     WMMDFNLRERDGGLFGTIKPSERNLSYTDQQKFHDEQPFLFRPPQGESISDLCQRIKI
                     VLDTLARECGGKDVIIVCHGHVMRAFRIILERMSLQKSNELMRATEEWSQTPNCSIIH
                     YTRENPFNNKQELSDRFDWVRMIRPAGGGNLEDDFSKIERKKYSNEELLLEAEKNRNT
                     K"
     gene            complement(8717..9478)
                     /locus_tag="P5056_01630"
     CDS             complement(8717..9478)
                     /locus_tag="P5056_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62450.1"
                     /translation="MKGVEGQMGKGKNYIENPKTKGSGILCCIPQKGVCPVGCADCFF
                     QSGRSFLEPLDENLPNMPSLKMSRGHVIRVNDGNDSNNNRELVLKNTEQYADKFYNTS
                     IPRDLAGFNAPVVLTVNPGHMTHSRFHELEVPPTNLMFVRVRVNTWNLDLVKYVVEHY
                     TSRKVPVVLTFLAYYKTGIPACEEKNYILRKRTLNEYRAITTEAWRGIMKMFEDNPLV
                     HSCGKIEGDKGSTGCKNCGNCLREYFATRERMKSV"
     gene            complement(9498..9971)
                     /locus_tag="P5056_01635"
     CDS             complement(9498..9971)
                     /locus_tag="P5056_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62451.1"
                     /translation="MVHYHEECARKCREAPVEEWEVILDVVVDEDTYPVKIAYFKHSI
                     YLRNLSCFSMFGYAWGHLFLKSCLAESNNIVTKEEIEKWCAKWGLRYLYDLRIRTLID
                     YFPERHGRLFELEPEKYEVLSTLEALQRFGIDALEQVGEKVVAYISEDYRPKIET"
     gene            complement(10152..10952)
                     /locus_tag="P5056_01640"
     CDS             complement(10152..10952)
                     /locus_tag="P5056_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62452.1"
                     /translation="MENIEKQQNKIENKFLPSLSLEQIDSQKEASILKGFVYETRFGP
                     LFNKRYPDLKKQLDEATGEENKIDICRKVLEEQREHNKDNIEKFEKEFIIEWKKIEND
                     FFTALSDLFETSWPIDKQITGSVTMMPIFPRFLNTYSFYIGYGSLPRNIETAAHEIVH
                     FLWFKKWKEIFPEISRNQYESPNLAWRLSEIIDPIILQCDPKIKELINPKGWGYSSFD
                     EIHIDGVKMTDYFKKIYLDSVASGDSFDDTMKKLWAEAQKHEKEISVF"
     gene            11319..11993
                     /locus_tag="P5056_01645"
     CDS             11319..11993
                     /locus_tag="P5056_01645"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRY62453.1"
                     /translation="MDKQIEYIAEALKLDLPDFECPDIGQTPAEIINNLKIVRQLDPK
                     VILEIGSATGGFFYMLSAALSNKKRTFISIDPWTEGTKYERQYHIYDETINNLKKFYP
                     NNQYFRIRRRSEDVRATEELKIMLKDQKIDFLFIDGAHTYQSVMNDWLNYSQFLNEKS
                     VVAFHDIVEYGEVNQAWKEISSQNNNYTHQEFKEEGIPLLTSLGETDAKFSKPIVLGV
                     GYLFKN"
     gene            complement(12071..12454)
                     /locus_tag="P5056_01650"
     CDS             complement(12071..12454)
                     /locus_tag="P5056_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62454.1"
                     /translation="MQKSRQIFSIREAVHFLLDFQGDYYGDSKERLVWLIPDHIIVQR
                     EDVPGYDPWKIIDSLEVLSLEKWHELAVIVGLGDWQKDKKQEKILFGLVNLDPTMQRA
                     LRPQIFEKIKEIRFLDAITYAVLHS"
     gene            12700..>12904
                     /locus_tag="P5056_01655"
     CDS             12700..>12904
                     /locus_tag="P5056_01655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62455.1"
                     /translation="MIQIPREDRKMLLRFVKEYNQHSHNDMAESAAKRNIEEGLLELF
                     DPLYFFDIKIPLLEMKRWRQHCSN"
BASE COUNT         3946 a         2718 c         2617 g         3623 t
ORIGIN      
        1 ggcaaagatg atataaaaaa gataatcgca atagaaaatt tcggcagacc caaaatactt
       61 tttctagacg agatacacag gttcaataag gcacaacaag acttcctcct gccttatgtt
      121 gagagcggca agataacttt tatcggcgcg acaacggaaa acccgagctt tgaaataata
      181 gcccctctac tttcccgatg tcgagtattt accttgaaag aacatggcga gaaggaaatg
      241 gcgaaaatta tagaccgcac gaaaataaag attggaaaag aggcaaaaga gtggctcatc
      301 aatatggcga acggcgatgc gcgctccgcc atcacgatgc tcgaaaatac gcagagattt
      361 tacggaaaga taaatataga aaatctaaaa cagaccctgc aggacaaatt tttgcgtttt
      421 gataagaaag gggaggaaca ctacaacacg acaagcgctt ttataaaaag tatgcgtgcc
      481 tcaaatccgg acgcggctct ctattatctc gcgaggatgg ttgccgccgg agaagatccc
      541 atattcatag cgagaagaat ggtgatattc gcatcagaag atattggtct cgcccagccg
      601 acggcgctag tcgtggccaa tgcggtgttc cgagcagtag aaaccgtagg atatcccgag
      661 tgctccataa acttagctca cggcgcgaca tatctcgcgc tagcagctaa aaacagagac
      721 tcgtataacg gccttcaaga agcgatgaag gatactgaga aatacggcaa cctacctata
      781 ccgctcaaaa taagaaatcc tgtcaccaag cttatgaaga atctcgaata tggcaaggga
      841 tacgaaaaat atacaaaaga agaccttttg ccggaaaaac taaaagggaa aaagtatctc
      901 aaaagaaggg aggatgagcc gcgtaagaaa taaggcttaa cgtcattgcg agccgaagcg
      961 tcttagctga ggcgtggtaa tccagaaatc ttaccgctaa attaaagctg gattgccacg
     1021 gacttcgtcc tcgcaatgac accatgtgat tttgaagatg cgcgggaaaa aatatttgat
     1081 ataataaagt tattataaat aattttttat tatgaatttt aaaacattct tcaaaagcga
     1141 aatggtttcc gccgcgaccg tgctcgcgat cggactcttt ctttcggcga tgggggccgc
     1201 atatactttt tatatgatta ggacctttga caacacactc tcgacgactg gctcagccaa
     1261 ggcaaaggtt acctccgatt cggttaaatg gagcgcctct atttcgagaa cggtcacaga
     1321 gggaaatata aaaagcggat atgccggctt aatagaagat cttgctatcg ttaaaaaatt
     1381 ccttacctct aaaggaattg ccgaatcaga aataaccaca accccggtat ttactaatga
     1441 ggtttataaa tacaacccat ccgggaatgc cggaccaaaa gaatttacct tgattcaaaa
     1501 cattacgatc aattccacag atgtcgcaaa gataaccgag gtggcaaaaa atacgcagga
     1561 tataatcaac aggggtgtat ttttccaaaa tcaatctcct gaatattatt attcaaaact
     1621 tgcggatctt cgcgttagcc tacttggaga tgcggtaaaa gatgcaaaag ctagagcgga
     1681 aatgctcgca aaaagcggcg gacgatcgat tggagccatg aaagccgcct caagcggagt
     1741 tgttcaggtg cttgctccaa actcgataga agtttctgat tacggacaat atgacacaca
     1801 gagcatcgaa aaggaggtga tggttactgc ccgtgcctcc ttcgtcgtcc gatagaatta
     1861 aaaaatttaa cacataacat cgtcgccggc tccgcttgtt cctcgcagta tgtgttatac
     1921 agcttcgtct caatgctcgc cgcccccttg ttctgtattt aaattattta attctatatt
     1981 cttgcgatgt ttaagaaact aaaggtaaaa agaatagata agtccataaa cctgcccaaa
     2041 taccagaccg gaggctcggt ggctttcgat tttatgtcca gaaaagacat cgagataaag
     2101 ccaaaagaga ttgcatatgt gccgctcaac gtctgcgtga aaattcccaa aaatcacact
     2161 ttgctaatgg cggccagaag ctctcttcat aaaaaggggc tatttatggc taacgatatc
     2221 ggggtttttg atgaagattt ctgcggtgac ggcgatgaat atcacgccgc gctatataat
     2281 tttagtgaca ggccggtaaa gatagaaaaa ggtgatagat taacacaagg ttttctcgtt
     2341 ccgattgcca aagttgagtg gaaagaggtg gacaaaatgg acacttgcga caggggcggt
     2401 ttcggtagta ccggatccaa ataaataagc ggcaatcaaa aatacggcct atttacgcga
     2461 tatccacacg ctattatttt acttcaactc ctaaatatga tatggtaaaa aaggtcggtt
     2521 ttttaacaat ttaccctcct aaatttttca cctttaatag gtgcttggta tttttcaatg
     2581 gacaaagtta aatccatcgt taatttacaa aggttaaaga aattttggcg ggttaaagcg
     2641 aaaatagcta tatgaatgag ctaatgttat ttattgtagg tatcgtggag atgataatcg
     2701 ttaccatctg gaccgagtcc gtcacaaagg caagagtcct agaaagtgga ataataacga
     2761 taatcaacat ctttatctgg tattacgtac tagatacgat agtaaatgac attgggaact
     2821 tcaacctggt tttggtttat gctctaggtt gttccacggg aacaatgcta ggcacccact
     2881 atatctcaaa gagagaggcc gcaaaggccc ttagagaagc caagtccgct taatccaaag
     2941 aaactggaga cagcgtaagt tcttaataat tttcatattt atatgaaaac agaagaaaac
     3001 gaaagttagc cgcccgattt gtctaaaaaa gtttttagat agattgggcg gctttctttt
     3061 ttatgaatta gaaataatgt aaaaatccag tcttctgctc gggccaagac atattacacg
     3121 ataaaaagtg ctaaactata catatggtag ttaagaaaaa gttaataatt attttgtttt
     3181 actttttgat catttctgtc gctatcggat ggatgataaa aaatattcaa gagcaagaaa
     3241 acctcaatgt tgtcgtattt tctccgaagg caaacgaggc gattagcagt ccttacaaaa
     3301 taaccggcaa ggctagaggc acctggtttt tcgaagcctc gtttccagta attttaagag
     3361 acaaaagcgg taaggagctg ggtattgcgg tagcacaagc aacctctgac tggatgaccg
     3421 aagattttgt gaacttcgta gctgaaataa agtttgaacc agcgggctta aaagaagggg
     3481 agttaatatt caaaaaagac aacccgtccg gcctacccga aaacgacgcc gaaatagttc
     3541 tccctgtaag atttaaataa aaaccccgac aaaacatcgg ggctttttta ttcacagggt
     3601 gcttgttaag ttttgtgacc taacacggta taatattagt atggacattc ttctatattt
     3661 tttgcgatat attatttggc actatactcg cgcgataaaa gaccttttta taaattggat
     3721 aaattttatc tggttcactt ttaatttctt ttccatttct cttctcctaa agacattctt
     3781 ttcaccatgg aaaaggctag gggaggcgcg aaaaggattt tttgatttca ctttcataat
     3841 catagacatc attatgcgcc tgttcggaat gctcctacgc tctattacga taatcatcgg
     3901 catggtttct gtgtgtgtcg tcatcctcgc aggcccgatt gctctcgccg tctggatagt
     3961 ctggccaatc gccataatat atttcttatc taagggctta actattttga tcaaatgata
     4021 aaaacaaacc caaatacagt aaggcgagcc tccttctctg gaattctggg gctagaaaaa
     4081 atatttccgc acaaaacaag acgaaaggtt tatctctttc taagaaacat aactatcgtc
     4141 atcggtcttt tgtatttggc gcttatattt gccgggggga atgaatcggt tacccttaag
     4201 accctcggcg tatttatggt atttttgggc gtcgcgatgg ccctattttc attcgaggcc
     4261 ttttttgctt catattatgg cggcaacgaa tccaaggttc tatccggtca aataattatt
     4321 tcttccaaag aaaatctttt gaaatcattc gcgcgttccc cattcggcct taaagtaatc
     4381 ctacgaacag aaatacctat tgaaatattc tcagaacttt ttaataaaaa tgagggcgaa
     4441 aacgaatttt tagatattgc aaaaggtgac tctcccggag atatactgaa tgcgaaagaa
     4501 cttgccttgg ccatttttga caaagaccaa gaccttaaaa aacttctcgc gtcatatggt
     4561 atagataaaa aaatatttga agacatttta gattgggcga ttgttgattt agaaaaagaa
     4621 taccgaaggg agctctggtg gtggcctgaa tatctgataa aaattccggg gattgggaaa
     4681 gactggaatt tcggccgcct attttatctc gtcaaatacg gaaaggacct tacccctgaa
     4741 gccacctcac caagtattga ttttgacata aaagagtttg gagtagaggt taaacagctt
     4801 gaaaatattt tgagcaaagg cagggaagct aacgctatgc ttgtcggtga accgggagtc
     4861 ggtgttctcg atatggttct tgagtttgta aggaagctaa atagaggcaa aatacacccc
     4921 gccctcgagt acaagagagt ttttttacta aactggaata ttcttacagc ctccaacaaa
     4981 gacaaaaaca gcctcgagag ggaacttata aacaccctta acgaagcact aggcgcaggg
     5041 aacattatcc tggtcataga tgaccttccg ggatttatgg cctctgcaga cgcccttggg
     5101 gtgaacgtgc agagcatttt ggacccttat ctggcttccc tcctccaaat actcgctacg
     5161 agcgacaacg gcaaattcca caaagtgatc gagaaaaatc ccgacctcat gagacgtttc
     5221 gacaaaataa acatagaaga gaccgacgaa gcaaagacaa tagaaataat agaaaatacc
     5281 gtcagaaaaa tagaaaaaac aaaggggcta tttttctctt atcctgcaat aaaagaaatt
     5341 gtgaccggcg caaatcaata tttccccggc acagttatgc ctgatggcgc gatagacctc
     5401 ctgtttgaaa taatcagttc ttcacttaga acaaacgact atatatccag aaacgatgtg
     5461 ctgaatttca tagaaatgaa aacgaagatt cctcttggca aaatggaggc gaccgaaaaa
     5521 gaaaaacttc ttcaccttga gccaatcctt catgaaagaa ttgtcggaca agaagaggcc
     5581 atatctgtta ttagtaatgc aatgagaaga acgagagcgg atattagaaa ctcaaagaag
     5641 cctatcggca cattcctatt cctcggaccg acaggtgtag gaaagacgga aacagcaaag
     5701 gcgctagcct cggtattttt cggtgatgaa cgatttatgc ttaggttaga tatgaccgag
     5761 tatcaaaaag ccgattctct gcaaagactc ataggttcat ttgaaagcgg taaacccgga
     5821 gtcctctcgg caatgcttcg cgaaaatcct tatggcgtgt tgcttctaga cgagtttgaa
     5881 aaagccgaga aagacgtgca aaacctcttt cttcagatac tcgacgaggg ctttttctca
     5941 gacatggacg gcaaaaaggt aagtcttagg aacgtaataa tcatcgctac atcaaacgcg
     6001 gcatccgact acatattcca gctggtgaaa gggggcaaag atcctgttgg cttgaaagac
     6061 gatgttgtcc aaaaaatagt ggacaatggc atcttcaaac cggaactcct gaaccgtttt
     6121 gatgcgtcag tgatatttca tccgctagat aagtccgaac tattccagat agccaaattg
     6181 atgctcatta agcttaagaa aaggctcgcg gaaaagggga ttgagtttgt ggtgaccgac
     6241 gagctggcta tggccgtagc taccgagggc gcgaacgaca tattcggcgc aagaccgatg
     6301 aatagatata ttcaagacaa aatagagcaa tttatagcca aaaagatgat agcaggggag
     6361 ctggcaggcg gaagcaaact agaaataaac ccgaaggagt tataaaaacg accaaaataa
     6421 agatcccgcc gaaaagcgga atttttgtgg ctgttgtaag ggcagtttca ggaatatata
     6481 ggcagatgga tcttcctctg gtaggaagac tgctggcagg ccccgaacac cggggctttt
     6541 tgtttattaa aacaaaaatc gcccaaaggc gattttgtgt ttatgaccct acggggatgg
     6601 aattggaacc ctgtggtgag aaatatgagg gaaatttgga ggtttaggta aaagaaaagc
     6661 ccccatcttg atagatgggg gcaattgcgt tgcaaattat ttatttcgct tagctaaatg
     6721 tttgttcagt tcgaaaccct ctccggtcca ataaccaaga ggatgacttt cggcgcccgg
     6781 gcaacatgcg aagcgcaaac agctgatgga cttgtcgcca atcatcgtcg cgaattcttt
     6841 tctaaaacgt tcgtggaggc gaccatcaac caatataact ccgttacccg gctcaatgcc
     6901 aatgccacag agacaacagc ggatcacttg tcttttagta ttgttaaaat agcaccgcgg
     6961 gcattcacag tcgtcgacag tcttctgccc gttgacatta ttggtttccc cgacgtcgaa
     7021 ctgtgtaatt ctaccgtgga aattgagctc ggccttctcg ggacccatgt ggccgcactt
     7081 cgtaaacttt actgttgcct tggttagagg tttatagcgc attggtacgt cgaaataatc
     7141 tttcggattt tccttctgtg atttgagcct cgccataaag acacatttgc ccttacgatg
     7201 caggccaacg atcttataat ttccgtccct gttaagattg ttatgggctt cttcttctga
     7261 tagaccccag aagtcggcga catttttgcc ggcagatttt atttgaatct tatacagacc
     7321 tttataccga cccttttcca cgtaaccttg ttcaacctta aaatacttgg tactcatagg
     7381 caacacctcc gattttatta aggaacctaa atctgagtcg tatactactg attttttata
     7441 gattgtaaag tgtgacctta cggggattcg aaccccgatt tccaccgtga gagggtgatg
     7501 tcctaaccat tagacgataa ggccaatgtt tgaattttta ctgcccgtat ataataaacc
     7561 atctttttca taaagtaaag aaaaagcgtc tgggtgaacc agacgcctgc gtgtttgcgg
     7621 ctctataaaa taatcaaatt aattcctatt ttgtgtttct attcttttcc gcctccaaaa
     7681 gcagttcttc gttcgagtat ttctttctct cgattttcga gaagtcgtct tcaagatttc
     7741 cgccaccagc cggccgaatc atcctgaccc aatcaaatct atcagacaat tcttgcttgt
     7801 tattaaaagg attttctctt gtgtaatgaa taatagagca attaggcgtt tgcgaccatt
     7861 cttctgttgc tctcatcaat tcattgcttt tttgaagaga cattctttct aaaataattc
     7921 tgaaggccct cattacgtgg ccgtgacaaa ctataatgac atcctttccg ccgcactctc
     7981 ttgccagggt atcgaggaca attttaattc tttggcataa atcggaaata ctttctcctt
     8041 ggggcgggcg aaatagaaat ggctgttcat cgtgaaattt ttgctggtct gtatagctca
     8101 gatttctttc tgagggtttt atggttccaa ataatccacc atccctctct ctgagattga
     8161 aatccatcat ccagcttgca ttatcgattt ccaaataggc cgccgtctgc attgcccgta
     8221 tattattaga gacgagcatt cgatcaaaca taatgaattt attttttctt aaccattctc
     8281 cggctttttt tgcttgttca atacccaatt tcgtgagctg atattgagat tcatgaagag
     8341 ccatgaattc atccgaaaaa taagattcgt cacccgtgtc cttaactcgt tttcttacaa
     8401 aatttccctc agattggccg tgccgaacga gaaacaggtt tttaggcatc ttcgtcttgt
     8461 ttgcggttaa ttttgacatc ggagcccctc cttttatttt tcaaagtatc aaaaactcaa
     8521 ctcaagatat caaataccca ataatttttc aacggaacct caaaattttc ccccaaaaac
     8581 tcagcaccac aaagttctca ttccgtcccc gcagggtcat cggattataa aaatgaaaac
     8641 cgccatttct ggcggtttga tgttgtcagc tacccatttt ctgttttgtg caaggataat
     8701 agtctgatca acgagttcag accgacttca ttcgctccct tgtcgcgaag tattcacgca
     8761 ggcagttacc gcagttttta cagccggtac tccctttgtc tccttcaatc ttgccgcagg
     8821 agtgcacaag aggattgtcc tcgaacatct tcatgattcc gcgccacgct tccgtggtga
     8881 tagcacgata ttcgttcaag gtcctcttgc ggaggatgta attcttttcc tcgcacgcag
     8941 ggatgcctgt cttataataa gccaagaaag tcaggacgac cggcaccttg cgagaggtgt
     9001 aatgttccac gacatacttc acgaggtcca aattccacgt attcacgcgc acccttacga
     9061 acatgagatt cgtcggtgga acctcgagtt catgaaaacg ggagtgcgtc atgtgtccgg
     9121 gattgaccgt gaggactacc ggagcgttga accccgcaag atcgcgagga atggacgtgt
     9181 tgtagaactt gtccgcatat tgctcagtgt ttttcaaaac gagctcgcga ttgttgttac
     9241 tgtcgttgcc atcattcacg cggataacgt gacctcgaga catcttaagc gaaggcatgt
     9301 tgggcagatt ttcgtccaat ggctcaagga acgatctccc tgactggaag aagcaatccg
     9361 cgcagccgac aggacaaacg cccttctgag gaatacagca caagataccg cttcccttag
     9421 tcttcgggtt ctcgatgtag ttttttcctt ttcccatctg tccctcaacc cctttcataa
     9481 gttctcaaag gacaatttta cgtctcgatt tttggtctat aatcctcact aatatacgcg
     9541 acgacttttt cgcctacctg ctcaagagcg tcaatcccga atctttgtag cgcttcaagt
     9601 gtagagagaa cctcgtattt ttctggttca agttcaaata gcctcccgtg tctttcggga
     9661 aaataatcaa tcagggtgcg aatcctcaaa tcatatagat atctgagacc ccatttcgca
     9721 caccattttt cgatctcttc tttagtaaca atattatttg attcagccag acacgatttc
     9781 aagaataagt ggccccatgc gtaaccgaac atcgaaaaac aggacagatt ccttagataa
     9841 attgagtgct tgaaatacgc aatcttgacc gggtaggtgt cttcgtctac cacgacgtcg
     9901 agaataacct cccattcttc gacgggggct tctctgcact tcctggcaca ctcctcgtgg
     9961 taatgtacca aacaagaatc ataacgatat ttttcaacaa ccggttatgc ccgccccttg
    10021 gatctctttc cagcaaaaag gacacctcat aggcataccc ctttctttca aggttcattt
    10081 gctcggctca ggatacaaca aaaatcttaa tttgcaaaat gagccttgtc gctacccaaa
    10141 cctccagacc cttaaaaaac actaatttct ttctcgtgtt tttgagcttc tgcccaaagt
    10201 tttttcattg tgtcgtcaaa actatcaccg gaggcaacgc tatctaaata tattttttta
    10261 aaataatctg tcatctttac tccgtcgatg tgtatttcat caaatgatga gtacccccag
    10321 ccttttggat taattaactc ttttattttt ggatcgcact gcaatattat tgggtcgatt
    10381 atttcactta atctccaagc taaattgggg ctttcatatt gattgcgtga tatttcaggg
    10441 aaaatctctt tccatttctt aaaccacaga aagtgtacta tttcgtgtgc tgccgtttca
    10501 atatttcttg gcaaagaccc ataaccgata taaaaagaat atgtattcaa aaatctggga
    10561 aagattggca tcatagtaac agaacctgta atttgcttat ctatcggcca agacgtctca
    10621 aataaatcgg aaagggcggt aaaaaaatca ttttctatct tcttccattc aataataaat
    10681 tccttttcaa atttttctat attatcttta ttgtgctccc tttgctcctc aagaaccttc
    10741 ctgcatatat caatcttatt ctcctctcct gtcgcctcat cgagctgttt cttcaaatcg
    10801 ggatatcttt tgttaaaaag tggcccaaac cgtgtttcgt atacaaaacc ttttaaaatg
    10861 ctggcttctt tctgagaatc aatctgttct aagctaagtg agggtaaaaa tttattttca
    10921 attttatttt gttgcttttc tatgttttcc atcgtctaaa ttttagcata aacacaataa
    10981 aaatatattt tgacccaagt cataatatct ggtaaattat ggataaattt aggccttcgt
    11041 aaatatggag aaaggagagc gtatgcttca aatcccggag gtttacctgg aggcgctctg
    11101 aaacatccta atttccctca aactcacgac caccggattc tgattctatc tttgcctgcg
    11161 caccacaggg caggcaggct cataaggtca caaaagaaaa agccccaatg gggctttcaa
    11221 tgtttctagc tgtgcaaaac gggataagtt atcgtgagca aaaacctaat ttccttaact
    11281 ttttatgcta aaatgtttaa tataaaaaag ctaaatttat ggataaacaa attgaatata
    11341 ttgcggaggc tctcaaatta gatctacctg attttgaatg ccctgatatt ggccaaactc
    11401 cagccgagat tattaataat ctaaaaatcg ttagacaatt agacccaaag gttattttgg
    11461 agataggttc tgcgacgggc ggatttttct atatgctttc cgcggcactt tctaataaaa
    11521 aaagaacctt catttcaata gatccttgga ctgaagggac aaaatatgaa aggcagtacc
    11581 atatatatga tgaaaccatc aataacttaa aaaagttcta ccccaataat cagtatttta
    11641 gaattcgaag aagatcagaa gatgttaggg cgacagagga actgaaaata atgctaaaag
    11701 atcaaaaaat agacttcttg tttatcgacg gagcgcacac atatcagagc gtaatgaatg
    11761 attggcttaa ttatagccag ttcctaaatg aaaaaagtgt cgtggcgttt catgacattg
    11821 tagaatatgg agaagtaaac caagcgtgga aggaaatatc ttctcaaaac aataattaca
    11881 ctcatcaaga atttaaagaa gaaggaatac cccttcttac ctctttgggc gaaactgacg
    11941 caaagtttag caagccaata gttttaggag ttggctatct gtttaagaat taaaccttaa
    12001 cttaatttta ttttacccaa attcccgtaa gctcacaaaa gaaaaagccc cgatggggct
    12061 tttaatgttt ctaactgtgc aaaacagcat acgttattgc atccaaaaac ctgatttcct
    12121 taattttttc aaatatctgc ggccgcaggg cgcgctgcat cgtgggatca agatttacaa
    12181 gcccaaacaa aattttttct tgttttttgt ctttttgcca gtcgccgaga ccgacgataa
    12241 cggccaattc gtgccacttt tctaaagaga gcacctctag cgaatcgata attttccacg
    12301 ggtcatagcc cggcacatct tcgcgctgga caataatgtg gtcggggata agccaaacga
    12361 ggcgttcttt agaatcgcca taataatctc cctggaaatc gagcagaaaa tggacggcct
    12421 ctcttatcga aaaaatctgg cgcgattttt gcatgctata cctccaaact caaagtgcga
    12481 actcttctca aactaccact caaaaataat acttacaacc tttgacaaag caataaaacc
    12541 tgttagattg aggctaaata ggacattgaa atttaaaggc cctccgaaac ttttggacac
    12601 agaaaccaag aggttaaata aaaacaagct agaaattata aatatctagt caaatcgcct
    12661 ttactgtgaa aagttttggc gggcaaaggg ggaatagcct tgattcaaat accaagggaa
    12721 gaccgaaaaa tgcttctaag attcgtcaaa gaatataacc aacattctca caacgacatg
    12781 gccgaatctg cggcaaaaag aaacatcgaa gagggattgt tggagctatt tgatccttta
    12841 tattttttcg atataaagat tcctctgctg gagatgaaaa gatggcgaca gcattgctct
    12901 aatg
//
LOCUS       DAOTEX010000017        12846 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3118
            NODE_3428_length_12846_cov_4.839184, whole genome shotgun sequence.
ACCESSION   DAOTEX010000017 DAOTEX010000000
VERSION     DAOTEX010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868142
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 12846)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12846)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 883
            CDSs (total)                      :: 833
            Genes (coding)                    :: 829
            CDSs (with protein)               :: 829
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12846
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3428_length_12846_cov_4.839184"
                     /isolate="AMR_MDS_3118"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1093
                     /locus_tag="P5056_01660"
     CDS             <1..1093
                     /locus_tag="P5056_01660"
                     /inference="COORDINATES: protein motif:HMM:NF012688.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HRY62456.1"
                     /translation="AGVEFNINSPKQMAEVLFEKLALPKKGIRKTAGGAISTKESELE
                     KLKDLSPVVAEILEYRGIQKLLSTYIDNLPLLVDENKRLHTHLNQTGTTTGRMSSTEP
                     NMQNIPARGEEGSEIRRAIIASPGYKLAAFDYSQIEMRVLAYLTDDETLIKTFREGKD
                     IHSSVASKVFGVPEDKITKEMRRQAKVINFGIIYGMGVKALKKNLGSTLEEAEAFHNN
                     YFEKFPKVKEYFERIKQEAHEKGYTTTYFGRRRYFPEINSHLPYIRSESERMAMNAPI
                     QGTATADIIKLAMSHVDGKLKEQNFSKDKVRLLLQVHDELVYEVKDDKDLPDIVGAIK
                     HAMENIVSINLPLSVNAYTGENWSEIKKV"
     gene            1178..1912
                     /locus_tag="P5056_01665"
     CDS             1178..1912
                     /locus_tag="P5056_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62457.1"
                     /translation="MYYLFYGKDTAKSRKKMAETVSSFQKKNPHSNIFRISDDDFSEA
                     AFLEHLGGQSLFSDKFLITLDGVLKNVEARDFIFENLPAVKESHNVFVFLEDDLKKAE
                     LAEIKKKAEKVFEFELPDDKGGGKGFNIFSISDAMGEKDKKKLWVLMNKAEREGLSSE
                     EIFWKLSWQVKNMLLAKQAISKGDRAIEKLKMSPFVLSKTKKYAEKFTEEELKSILGK
                     IVSLYHESRRGFFDFDTALEGFVLSL"
     gene            2207..2359
                     /locus_tag="P5056_01670"
     CDS             2207..2359
                     /locus_tag="P5056_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62458.1"
                     /translation="MDQEWLMEEVEAVATVYLEAKGFDENGRDKFDPTQDEFEREDIT
                     LMGLVW"
     gene            2500..3378
                     /locus_tag="P5056_01675"
     CDS             2500..3378
                     /locus_tag="P5056_01675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62459.1"
                     /translation="MSKNQGGNNTKSEKKAYEQFVVFKGDTTDTTFLKQGTERLEAGK
                     KFRLRQIEDLTGTEIEADGANLIPVSDEKRKAIIAKHGFSPDYRQVDPNPGYVIGNLD
                     TPPAVVMQITKDHLVLEVPQTDENGKQSTTTVTIGKKSARALPGQTIDEVKKKYRLLL
                     EKLEDKRRAEKMEKQKREEERKHFRAFSGGVYESPYHGVNGAANETPLGLILVLSSYD
                     GVKGTDDRWANVRRVLKTETGYELDKDSVVAAVYLDQNGSKPTQCMFPTDPSVVEKVR
                     NMPEVREVLAKLQTRR"
     gene            complement(3879..3951)
                     /locus_tag="P5056_01680"
     tRNA            complement(3879..3951)
                     /locus_tag="P5056_01680"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3916..3918),aa:Lys,seq:ttt)
     gene            complement(4046..5494)
                     /locus_tag="P5056_01685"
     CDS             complement(4046..5494)
                     /locus_tag="P5056_01685"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRY62460.1"
                     /translation="MKWEVLDHKSPNGEELECLDFLNKRVIGQDRAKMAIVRNYIAWK
                     HGVRNKNRPPGIFIFLGPSGVGKTSLVAALAEMIFDRSDAFLKVDCSTFQEKHDGARL
                     LGAPPGYIGHDGEPELTQEKLDIHGAVKFGAEEKKTPKAPSKEEIAQKITGIKDRLRV
                     KNDMLQDINSNINRQYNRLNFSVLSEKESAETVEYLATLAIKRRITQKDIVDLGDEFL
                     RIIDGKDEPEKIEIRETMPPPSILLLDEFEKAHPEITKQFLPMFDTGIMPRANGKKID
                     FRNCFIFMTSNLGSDAVRRALEKEKGIGFVTSSSEPKKEEITSPKAIYRIVMREIRKS
                     PVFTPEFLGRIGKKNIIVFHSLEREIINQIIQRRLLAIRAEINAYIPGVRISFKNELV
                     EYIMGKAMDAENRTLGARPVETITTKEILEPLFSLAIKGEDGGVIARDEVIVTVSCAK
                     EAGDEKEVIFLRNRNPDIGSDFPEEYRPKSDA"
     gene            complement(5750..6145)
                     /locus_tag="P5056_01690"
     CDS             complement(5750..6145)
                     /locus_tag="P5056_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62461.1"
                     /translation="MEILTKYFGFKKILSIFPELYNYAGYSPLLLRLSVGVIYFGDGL
                     QDLKKKSGTKIFNTTLGVLKIISGLFLFFGLYAQLWAIVAICLSIFNIFLSVGEGNWQ
                     KLKYNFLILTITASLMLSGPGLYALDLPL"
     gene            6267..6755
                     /locus_tag="P5056_01695"
     CDS             6267..6755
                     /locus_tag="P5056_01695"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HRY62462.1"
                     /translation="MKAKNKTKELYLILDDIRSNFNVGSIFRIADCVGIKKIFLGGIT
                     PAPLDRFGRINNEIKKVALGADETVSWEKAPSVSLLVNKLKKDGFEIVSLEQSEDSTD
                     YKKFKLKKKTALIVGNEVAGVKKSLLKLSDHILEIPMMGDKESLNVAVATGITVFRIL
                     NV"
     gene            complement(6879..7778)
                     /gene="mutM"
                     /locus_tag="P5056_01700"
     CDS             complement(6879..7778)
                     /gene="mutM"
                     /locus_tag="P5056_01700"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR00577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HRY62463.1"
                     /translation="MPELPEVTTIVFGLNKKVKGLTIRDIWTDWPKTIKSHSFTEFKK
                     EVIGKKILTSERVGKNVLTHLSGNKTILFHMKMTGHLLYGKWKLKKEGDKNIWKPEKE
                     GTPLSDPYNRFIHIIFYLSNGYELAFSDVRKFGKIVLLEEGKEFESKDLKHLGPDPMK
                     KDFNFAKFKKALSKKKDGAIKQILMAQEIISGIGNIYSDEILWRAGINPKERLKKLNE
                     KDLRNIFTATKDILGRSIKMGGDSMSDFRNIEGEKGSYQKCHLVYRQTGKACNKKGCK
                     GIIRRTKVGGRSAHFCETHQALK"
     gene            complement(7820..9154)
                     /gene="murE"
                     /locus_tag="P5056_01705"
     CDS             complement(7820..9154)
                     /gene="murE"
                     /locus_tag="P5056_01705"
                     /inference="COORDINATES: protein motif:HMM:TIGR01085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramyl-tripeptide synthetase"
                     /protein_id="HRY62464.1"
                     /translation="MQEKLEKIKKMLPRTMLEQMRPLYHYALSYTGKLVYNNPSKDIF
                     VIGVTGTKGKSSTAEILNAILEEAGYKTAILSTVRFKIGDETEPNLMKMTMPGRFFVQ
                     KFLRRAVDKKCDYAIVEMTSEGAKNYRHRFVDMNALIFTNISPEHIESHGSFENYLGA
                     KLQIAKRLEHGSKPSKTIVVNEDDDKAGKFLAINVPNKITYSIKEAKPFTLTKEGSDI
                     NLFGVRVQTSLPGEFNVYNILAAAKFAQSMGVKTETIKKAIEKLKTIPGRLEKIEISL
                     AHLKFEGNKKPVGLGIFPNFSVIVDYAHTADSLEKVYKVFKNERKICVLGSCGGGRDK
                     WKRPLMGEVADKNCDEIILTDEDPYDDDIEEIIQCVASGVKQHKPKIILDRREAIREA
                     LRTAKEGDAVIITGKGTDPFIMGPNGTKKVWSDKKVAEEELIALALERNSKS"
     gene            complement(9201..9773)
                     /gene="ruvA"
                     /locus_tag="P5056_01710"
     CDS             complement(9201..9773)
                     /gene="ruvA"
                     /locus_tag="P5056_01710"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HRY62465.1"
                     /translation="MIAQIEGKITHIGDKFLIINVSGLGFKVFVTKDLLFQFEKEGAK
                     NVSLHTYLSVKEDALDLYGFENLEDLNFFEKIISVSGIGPKKALGIMSIAPVQTLKKA
                     ISSKDISYLTQVSGIGAKNAEKIVLELRDKMKGEDGDGELLKEESDVIMAIKSLGYTA
                     NDARKALKNIPADIKGVSQRVKWVLKELGK"
     gene            9945..10214
                     /gene="rpsT"
                     /locus_tag="P5056_01715"
     CDS             9945..10214
                     /gene="rpsT"
                     /locus_tag="P5056_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012858563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HRY62466.1"
                     /translation="MAITKSAKKAARASLRKRVFNQRRAGAMRSSIKDVRKLVTAKDL
                     DAAKKNLSEAYKAIDKAMKKGVIKKNTAARKKSRLAKLVKKASGK"
     gene            10287..10358
                     /locus_tag="P5056_01720"
     tRNA            10287..10358
                     /locus_tag="P5056_01720"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:10320..10322,aa:Arg,seq:gcg)
     gene            complement(10494..11003)
                     /locus_tag="P5056_01725"
     CDS             complement(10494..11003)
                     /locus_tag="P5056_01725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62467.1"
                     /translation="MYNNEPEILNPIRGTTEISWETLEHEHIPVSNDWYWTIGLVAAI
                     SAVASFYYGNIVFGAFSIVAGLTMIMFKIKGPEHIKVRLTPRGIIVKNSLYPYGRIES
                     FWIYEDSGQLEQMNELSIKSDRAFLPQIIIPLGDADVELVKDYLSRYIKEEYHEKTLA
                     DVVSEYIGF"
     gene            11191..11275
                     /locus_tag="P5056_01730"
     tRNA            11191..11275
                     /locus_tag="P5056_01730"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11223..11225,aa:Ser,seq:tga)
     gene            11353..12003
                     /locus_tag="P5056_01735"
     CDS             11353..12003
                     /locus_tag="P5056_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62468.1"
                     /translation="MAHRPYSDKIWFSPRYVIQEWANIERSGDKKKIKRAREAWVCAV
                     AMTCHSKIEPVEWWIQIPQNDPPDVLAMKLVPHVKGLGNTMEQLQVEVFEISEHDTED
                     IEKSIERKLGEKDYSGMTLIAFVRRKQVFAHEKVATFIQNLKPKALAIFLVANEATQG
                     TNFSFIGIFPQVFKYQCDWGAMCKVANQNDFTTATRGTKVPEKTTDYTADTMTLVP"
     gene            complement(12005..>12846)
                     /locus_tag="P5056_01740"
     CDS             complement(12005..>12846)
                     /locus_tag="P5056_01740"
                     /inference="COORDINATES: protein motif:HMM:NF019149.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HRY62469.1"
                     /translation="GPGRRAGRWRWPGPAPTGYMKEKRTDRKCETLLDPERAPIIKKM
                     FEKVAYEHWSGRKIYNWLKFDLNMRTAAGKKHLSLGNIYKILDNTFYYGVFEYPRKSG
                     NWYTGKHEAIVTKELFDLVQSQVKSNVLRIENKEFAFTKMMFCGLCGSGISADEKFKK
                     LKNGGVARYVYYGCTKARGVDCKCGYTEEKEVIKQFNELIEKINLNEIEVKEKIKADV
                     ERFSKLQKFLLGNKEKVSADSIDVRGYAKFVLKEGSDIEKRELLGCLKSRICLANKKI
                     SLK"
BASE COUNT         3488 a         2742 c         2812 g         3804 t
ORIGIN      
        1 ggcgggagtg gagtttaata taaattctcc caagcagatg gccgaggtgc tttttgaaaa
       61 gttggcgctt ccgaagaagg ggataaggaa aacggcggga ggggcgatat ctacaaaaga
      121 atccgagctg gaaaaattaa aagatttgag tcctgttgta gcggaaatat tggaatatag
      181 gggaattcag aaattacttt ctacatatat agataatctt cctcttttgg tagacgaaaa
      241 caaaagacta cacacgcacc tcaatcagac cggcacaacg acaggcagaa tgtcttccac
      301 tgagccgaat atgcagaata ttccggcgag gggagaggag ggttcggaga taaggcgggc
      361 gataatcgct tctccgggat ataagctggc cgcttttgat tattctcaga tcgaaatgag
      421 agtgcttgct tatctcactg acgacgaaac gcttataaaa acattcaggg aagggaaaga
      481 tatacattca tccgtagcct caaaagtttt tggagttccg gaagacaaaa taacaaaaga
      541 aatgcgacga caggccaagg tgattaactt cggaattatc tatggaatgg gcgtgaaggc
      601 tcttaagaaa aatctcggtt ctactctcga agaggcggag gcgttccata ataattattt
      661 tgaaaagttc cctaaggtaa aagaatattt tgaaaggatt aaacaagagg cacacgaaaa
      721 aggttatacg acaacatatt ttggaagacg cagatatttt ccggagataa attctcattt
      781 gccgtatatt cgatcagagt cggaaagaat ggcgatgaac gctccgatac agggcaccgc
      841 tacggccgat attattaaac tcgcgatgag ccatgtggac ggtaaattaa aggaacaaaa
      901 tttttctaaa gataaagtca ggttgcttct gcaagttcac gacgagcttg tttacgaagt
      961 gaaggatgat aaagatttgc cggatattgt aggagctata aaacatgcga tggaaaatat
     1021 cgtttctata aatctcccgc tttcagtgaa cgcttatacg ggagaaaatt ggagcgaaat
     1081 aaaaaaagtg taaagcgata agcgaaaaat gcaaaagaaa aatatttaag tttggtttgt
     1141 gcttaattct ttattcttac ttcttaattc taatataatg tattatcttt tttacggaaa
     1201 agacacggca aaaagcagga aaaagatggc ggaaaccgtt tcctcttttc agaagaagaa
     1261 cccgcattca aatattttta gaatttcgga cgatgacttc agcgaagcgg cctttttgga
     1321 gcacctcggt ggacaaagcc ttttttccga caaatttctt atcacgctag acggggtttt
     1381 gaagaatgta gaggcgcgag attttatttt tgagaatctt ccggctgtaa aggaatcaca
     1441 taatgttttc gtttttcttg aagacgacct gaagaaggcg gaacttgcgg agatcaaaaa
     1501 aaaggcggaa aaagtttttg aatttgaact tccggacgac aaaggggggg gaaaggggtt
     1561 caatattttt tctatatccg atgcgatggg agaaaaggac aagaaaaaac tttgggttct
     1621 tatgaataag gcggaaagag agggtttaag ctctgaagag attttttgga agttgtcttg
     1681 gcaggtgaag aatatgcttc ttgcaaaaca ggctatatcg aagggggata gggccatcga
     1741 gaagcttaaa atgagtccat ttgtcctctc aaagaccaaa aaatacgctg aaaaattcac
     1801 agaagaggag cttaagagta ttttgggcaa gatagtctct ctttatcacg agtccaggcg
     1861 cggttttttt gattttgata cagcgttaga aggttttgtt ctcagtcttt agacttttcc
     1921 taaaatatcc ttattttagg ctatgttatt ccgattttta attaaaaagc ggggtggatt
     1981 tgacattttc tatctactat gctatactta caaaagttaa ttaagaagtt cttttccata
     2041 ttttaggttt ataaaaaaaa taaatatacg gaaggggtgt ttggcatgat gggccacgaa
     2101 caagaattgg acgacgatcg cgagattctg agtgaccacc tcaaggagat gctggcccgg
     2161 atcgagaagt agtcacccga agcaaaagac agaagggagg taaccgatgg atcaggaatg
     2221 gctgatggag gaggtcgagg cggtcgcgac tgtgtatctc gaagccaagg gcttcgacga
     2281 gaacggccgc gacaagttcg atccgactca ggatgagttc gagcgtgagg acatcaccct
     2341 catggggctc gtctggtaga gatgacgaag gtcatcaaac attagctggg ccgatcatcg
     2401 gtcctcatac aggtcacccc aattttctga gggtgcggtt ctaaaacatt ggcgacttcg
     2461 gcaagcttgt cggggtccat ctcatactgg aggaacgcca tgagcaagaa ccagggcggc
     2521 aacaacacga agagcgagaa gaaggcctac gagcagttcg tcgtcttcaa gggcgacacc
     2581 accgacacca ccttcctgaa gcagggcacc gagcggctcg aggccggcaa gaagttccgg
     2641 ctccgccaga tcgaggacct cacgggcacg gagatcgagg ccgacggcgc gaacctgatc
     2701 ccggtctcgg acgagaagcg caaggcgatc atcgccaagc acggcttcag ccccgactac
     2761 cggcaggtgg atcccaaccc gggatacgtc atcggcaacc tggacacccc gcccgcggtc
     2821 gtcatgcaga tcacgaagga ccacctggtc ctcgaggtcc cgcagactga cgagaacggc
     2881 aagcagtcga ccaccaccgt caccatcggc aagaagtcgg cgcgcgccct cccgggtcag
     2941 accatcgacg aggtcaagaa gaagtaccgc ctgctcctcg agaagctcga ggacaagcgc
     3001 cgcgccgaga agatggagaa gcagaagcgc gaggaggagc gcaagcactt ccgggcgttc
     3061 tccggcgggg tctacgagtc gccctaccac ggcgtgaacg gcgccgcgaa cgagacgccc
     3121 ctcgggctca tcctcgtcct ctcctcctac gacggcgtga agggcacgga cgatcggtgg
     3181 gccaacgtcc gccgcgtcct gaagaccgag acgggctacg agctcgacaa ggactccgtg
     3241 gtcgccgcgg tctacctcga ccagaacggt tccaagccca cgcagtgcat gttcccgacc
     3301 gacccgagcg tcgtcgagaa ggtgcgcaac atgcccgagg tcagggaggt cctcgcgaag
     3361 ctccagaccc gccgctaaac ctaaaatgca acgttcgtcg aaataacatt cgaacgaacc
     3421 caacaccgcc gcccttcggg gaggcggttt tttattgggg gaattaagat actgggctaa
     3481 agcccagcag gaatcttccc ctacggggac agtaggggtc agagcaggaa tcggccacga
     3541 atatttttgt tacacaaaaa tttctcggcc ccggctcggc ttccattctt gcagaatgga
     3601 tcctgcctcc gcccttcgat tcctgctgga gcgctacaaa acaaaaacac ccaaatgctt
     3661 tgcatttaaa aagaaggaat cggtccttac aggaccagtg cccacataag gaacacagga
     3721 atcggccaca agttactaat tcactttgtt cataagtact tgcggcccca cctcagctcc
     3781 ctttcttgta gaaaggggcc cgcttccggt tttcgattcc tgctgggcaa gcaaaataaa
     3841 accactcgaa tgctttcgca ttcgaagtgg ttattttgtg cgcccagcag gaatcgaacc
     3901 tgcgaccatt tgcttaaaag gcaactgctc taccagctga gctatgggcg cctatacgcg
     3961 aagaaaacaa taacaaaaaa tattcctatt gtctatatta tacaccaata agaaacccgc
     4021 cacatctgtg gcgggtaaat ataaattacg catcggattt cggtctatat tcctcgggaa
     4081 aatcggaacc gatgtccggg tttctgtttc taaggaaaat aacctctttt tcgtcgccgg
     4141 cttctttggc gcaagagact gtgacgatca cttcgtctct cgcgattacg cctccgtcct
     4201 cgcctttgat tgcgagactg aagagaggct cgagaatttc tttggttgtg atggtttcga
     4261 cagggcgcgc gccgagggtt ctgttttcgg cgtccatcgc cttgcccata atatattcca
     4321 caagctcgtt tttaaaagag attctgacgc cgggtatgta ggcgttaatt tccgcgcgaa
     4381 tcgctaagag gcgccgctgg ataatctggt taataatttc tctttcgaga gaatgaaaaa
     4441 cgatgatgtt tttcttgccg attctgccga gaaattccgg ggtgaaaaca gggcttttcc
     4501 taatctctct cataacgata cggtagattg ccttggggct cgttatttct tccttcttcg
     4561 gctcgctgct actggttacg aagccgatac ctttttcttt ttcaagcgcc cttcgcaccg
     4621 catcggagcc gaggttggag gtcataaaga tgaagcaatt tctgaaatcg attttcttgc
     4681 cgttcgctct cggcatgatg ccggtgtcga acatgggcag gaattgtttg gtaatttcag
     4741 ggtgagcctt ttcgaattcg tcgagaagaa gaatgctggg aggcggcatg gtttctctaa
     4801 tctcaatctt ttccggctcg tccttgccgt caataatcct caagaattca tcgcctaagt
     4861 cgacaatatc tttctgggtg atgcgtctct tgatagcgag agtcgccaaa tattcgacgg
     4921 tttccgcact ctctttttca gagagaacgg agaaattaag acgattgtat tgtcgattta
     4981 tgttggaatt aatatcttga agcatgtcgt ttttgactct aagacgatct ttgatgccgg
     5041 tgatcttctg agcgatctct tctttactcg gtgccttcgg ggttttcttt tcttcagcgc
     5101 cgaatttgac ggcgccgtgg atatctaatt tttcttgagt taattccggc tcgccgtcat
     5161 gtccgatata gccgggaggg gctccgagga gtctcgctcc gtcatgtttt tcctgaaagg
     5221 tcgagcagtc gactttcaag aaagcgtcgc ttctgtcgaa gatcatttcg gccagagcgg
     5281 cgacgaggga tgtttttccg acgcccgacg ggccgagaaa aatgaaaatt ccgggaggcc
     5341 tgtttttgtt cctaacgccg tgtttccacg cgatatagtt gcgaactatc gccattttgg
     5401 cgcgatcctg accgataact ctcttgttca aaaaatccaa acattccagt tcttctccat
     5461 tgggagactt gtgatcgaga acttcccact tcataattac caccctttct tgcgacctta
     5521 cggccgtcca tgcgttattt acccgccaaa attttcggat atttataatt tagttgatat
     5581 ttttataatt caactattta agtatttgtt agtgagggtc gttttgttgg tgaaaaattt
     5641 aggagggtta atttcaaaga acgttgcctt atctaatcct atctagtaac aaaatgttgt
     5701 caacgctaaa agaccgcctt aacatatggg gcggtctttt ttaacttttt taaagcggca
     5761 ggtccagcgc gtataatccc ggaccggaaa gcatcagaga ggcggttatt gtgaggatga
     5821 gaaaattata ttttagtttt tgccagtttc cttcccccac agaaagaaag atattaaaaa
     5881 tagaaagaca gatggcgaca atcgcccata gttgagcata tagtccgaag aacaaaaata
     5941 gcccggaaat tatttttaac accccgaggg tcgtgttaaa tatttttgtg ccacttttct
     6001 tttttagatc ttgtagtccg tcaccaaaat aaattacccc tacagacaat cttaagagta
     6061 ggggggagta tcccgcgtag ttgtataatt cggggaatat gcttaatatc ttcttgaagc
     6121 cgaaatattt tgtgagtatt tccatattta ccctgtttag taagatttaa ttaatactta
     6181 aatcttatca catttaaata ttaattaaat ctaaaattaa ttatttttca taattaattt
     6241 ttacttaaca gggtgaattc taccccatga aagctaaaaa taaaaccaaa gaattatatt
     6301 tgatactgga cgacatccga agcaatttta atgtcggctc aatttttcgt atcgccgatt
     6361 gcgtaggcat aaagaaaata tttttgggag gaattactcc cgcgccgctt gatagattcg
     6421 gaagaataaa taatgaaata aaaaaagtgg cgctaggggc cgatgaaacg gtttcttggg
     6481 agaaggcgcc gtctgtttca ctcctcgtaa ataaattaaa aaaggatgga tttgaaatag
     6541 tctctttgga acagtccgag gattctacgg actataaaaa atttaagtta aagaaaaaaa
     6601 ctgcccttat tgtcggcaat gaggtggcgg gtgttaagaa aagtttgtta aaactgtccg
     6661 atcatattct tgagataccg atgatgggag ataaagaatc tcttaatgtg gcagtggcta
     6721 ccggaatcac tgtctttaga attttgaacg tttgaccgtt tttctgtttc ttcttctttt
     6781 tgtcattccc gcgcaggcgg gaatccatct tttaatttta ggcatactgg attcccgcct
     6841 gcgcgggaat gacaagaaaa catggcgatc aaaaagtttt atttcaaagc ttgatgcgtt
     6901 tcacaaaagt gggcgcttct acctccgact tttgtccggc ggatgatacc tttgcaccct
     6961 tttttgttgc aggcttttcc cgtctgtctg tatacaagat ggcatttttg atacgagccc
     7021 ttttctcctt caatatttct gaaatcagac atagagtctc ctcccatttt tattgatcgt
     7081 cccaagatat cttttgtcgc agtgaatatg tttctcagat ctttttcgtt taattttttc
     7141 aaacgttctt ttgggtttat tccggctcgc cagagtattt cgtcggagta gatatttccg
     7201 ataccggaga tgatctcttg tgccatcaaa atttgtttga ttgcgccgtc tttctttttt
     7261 gatagcgcct ttttgaattt cgcgaaatta aaatcttttt tcatcggatc ggggcccaga
     7321 tgtttcaggt cttttgattc aaattctttt ccctcttcca acaaaacaat ttttccgaat
     7381 tttcttacat cggaaaaagc taattcgtag ccgttcgaaa gataaaaaat aatgtgaatg
     7441 aatctattgt atggatcaga aaggggagtg ccctcctttt ctggtttcca gatatttttg
     7501 tctccttctt ttttgagttt ccattttccg tagagaaggt gccctgtcat cttcatatga
     7561 aatagaatgg ttttgtttcc ggaaagatga gttaggacat ttttcccgac tctttcggat
     7621 gtgagtattt ttttacctat gacctctttt ttaaattcag taaaagaatg tgactttatg
     7681 gtctttggcc agtcggtcca aatgtccctt attgtcagtc ctttaacttt tttatttaga
     7741 ccaaaaacaa tagttgtaac ttccggtaat tcgggcatag aaaattaatt aaaaaattat
     7801 caattacgaa ttacgaaaat taggatttag aatttctttc taacgccagc gcaatgagtt
     7861 cttcctccgc cacttttttg tcgctccaaa cttttttggt tccattcggg cccattatga
     7921 acggatctgt gcccttgccg gttataatta cggcatctcc ctccttcgcg gttcttaatg
     7981 cttctcttat tgcttctctt ctgtctaaaa taattttagg tttgtgctgt tttactccgc
     8041 tagcgacgca ttgaattatt tcctctatgt catcgtcata agggtcctcg tcggtaagaa
     8101 taatttcgtc gcaattttta tcggcaactt cgcccatgag aggtctcttc cacttgtctc
     8161 tgcctccacc gcaagagccc aatacgcaga tttttctttc gtttttgaat actttgtaaa
     8221 ccttttccaa agagtcggcg gtgtgggcgt agtcaacaat cacggagaag ttcgggaaga
     8281 ttcctaagcc gacgggtttt ttgtttcctt caaatttcaa gtgtgccagg cttatctcta
     8341 ttttttctaa cctccccgga atagttttta gtttttcaat cgcctttttt atggtctcgg
     8401 tctttacccc catgctctgc gcgaattttg cggccgctaa aatgttgtag acattaaatt
     8461 ctcccggcag gcttgtctgc accctgactc caaataaatt aatatcagat ccttcttttg
     8521 ttagagtgaa aggttttgct tcttttattg aatatgtaat tttattcggc acatttatcg
     8581 caaggaattt tccggcctta tcgtcgtcct cattcacgac aatggtcttg cttggcttgc
     8641 taccatgttc gagccttttt gctatttgaa gcttcgcgcc gagataattt tcaaaagagc
     8701 cgtgcgactc aatgtgctcg ggggagatat tcgtgaaaat gagcgcgttc atgtctacga
     8761 atcggtgtct gtagtttttt gccccttccg acgtcatctc tacgatagcg tagtcgcatt
     8821 ttttatctac cgcccggcgc aagaattttt ggacgaagaa ccttcccggc atggtcattt
     8881 tcatcagatt tggttctgtt tcgtcgccta ttttaaatct aaccgtagag agtatggcgg
     8941 tcttgtatcc ggcctcttcc agtatcgcgt ttaatatttc cgccgttgac gattttcctt
     9001 ttgtgcccgt aacgcctatt acaaaaatat cttttgaggg attattatag accaactttc
     9061 ccgtgtatga aagagcgtaa tgataaagcg gcctcatctg ttccagcatg gttcgcggaa
     9121 gcattttttt tattttttca agtttttctt gcatattctc tactgtaatt attatctatt
     9181 ttctattctc tatcttctaa ttattttcct aattctttca acacccattt aactctttgc
     9241 gaaacccctt ttatgtccgc aggtatattt tttagcgcct ttctcgcatc gttcgcggta
     9301 tatccgagag acttgattgc cataataacg tcagactcct ccttgagcaa ttctccatcg
     9361 ccgtcttctc ctttcatttt gtctctaagc tcgagaacta ttttttcggc gtttttggcg
     9421 ccgatgccgg acacttgcgt aaggtaagaa atatcttttg atgagatagc cttcttcaac
     9481 gtctggaccg gggcgataga cattattcca agcgctttct tggggccgat gcccgagacc
     9541 gaaataattt tttcaaaaaa attaaggtcc tctaaattct caaaaccata taaatctaga
     9601 gcatcctctt ttacagagag ataagtgtga agtgagacgt ttttggcgcc ttctttttca
     9661 aactggaaca gaaggtcttt tgtcacaaat accttaaaac cgaggccgga gacatttata
     9721 atcaagaatt tatcgccgat atgcgttatt ttaccctcaa tttgagcaat catttaataa
     9781 aggaattatg aattaagaat tatgagttaa gaataacaaa gccaagccaa tattgcgagt
     9841 cctggggtgg ccttgctttt tacaggatat taatgtagta tcatttcaat aacgaaaacg
     9901 actttaactc gtaattcgta attgttaatt cttaatttat ttagatggca ataacaaagt
     9961 cagcaaagaa agcagcgagg gcctctttga gaaagagggt cttcaaccaa agaagagccg
    10021 gagccatgag atcttctatt aaggacgtta gaaaattggt taccgctaag gacctcgatg
    10081 ccgccaagaa gaacttgtca gaggcttata aggcaatcga taaggccatg aagaaggggg
    10141 ttatcaaaaa gaacacagcc gccagaaaga aatcaagatt ggccaagctc gttaagaaag
    10201 cttccggaaa ataacaaatt taaaaaacac ccctagtggg gtgtttttgt ttacttattc
    10261 ttttgtttta ttatagtgta tattatcctc tcgtagttca atggatagaa taggagcttg
    10321 cggagcttct gatgtaggtt cgattcctac cgagaggatt gagagcaagg cgcctgcgcg
    10381 ccttgcgtgt aggaatcgaa aaggttctcc gttatatttt agaaatttta atttctaaaa
    10441 tatcggaaac ctataccgtc cccgtagggg aagattcctg ccgagaggat tgtttagaag
    10501 cctatatact ctgagacaac atctgcgagt gttttttcgt gatattcctc tttgatatat
    10561 ctggagagat aatcttttac cagttcaaca tcggcgtctc cgagcggaat tattatttga
    10621 ggcaaaaagg cgcgatcaga ttttattgaa agctcattca tctgctccag ctgaccgctg
    10681 tcttcatata tccagaatga ctcgattctt ccgtaagggt ataaagagtt ttttactata
    10741 attcctcgtg gggttaaccg aaccttaatg tgttccgggc ctttaatttt aaacattatc
    10801 atcgtgagac cggccacaat ggagaatgct ccaaaaacta tgtttccata gtaaaaagaa
    10861 gctacggcgg aaatagcggc cacgagaccg atggtccaat accagtcatt tgagaccgga
    10921 atgtgttcgt gttctagcgt ctcccacgag atctccgttg tgcctctaat tggatttaaa
    10981 atttccggtt cattgttata catgttcaaa ttataacatc ccattaaaag ttcgacaaaa
    11041 ttatgcggtc tgttttttgt ttgtctgcga agcgcctgcg gcttcgataa gagttaggca
    11101 tctttagtct gtaaactttt aacgggacag gcacacctct ttaatttttg tccacattct
    11161 gctagtatcc taaaggcctg aagaaaacac ggagggttcg cctaatggta tggcaatagt
    11221 cttgaaaact atcgggtgaa agcccttaga ggttcgactc ctctaccctc cgccaagtcg
    11281 tgatagcacg acatgcatac aaaaagcact gatttcagtg ctttttgtat ttgataaatg
    11341 tatgatgaat ttatggcaca cagaccctat agcgacaaaa tatggttttc tcctcgttat
    11401 gttattcagg aatgggcgaa tatagaaaga tctggcgaca agaagaaaat caagagagca
    11461 agggaggcat gggtttgcgc cgttgccatg acatgccatt caaaaattga acctgtagaa
    11521 tggtggattc aaattcccca aaatgatccg ccagatgttt tagctatgaa actagttcct
    11581 catgtaaaag gattgggcaa tactatggag caactacaag tggaagtttt tgaaatcagc
    11641 gagcatgata ctgaagatat tgaaaaatct atcgagagaa aactcggtga aaaggattat
    11701 tcaggaatga cacttatcgc ctttgttaga aggaaacagg tttttgcaca cgagaaagtc
    11761 gctactttca ttcagaatct aaaaccaaaa gctctcgcta tttttcttgt cgcaaacgag
    11821 gctacacaag gaaccaactt cagttttata ggtatctttc ctcaagtgtt taagtatcag
    11881 tgtgactggg gcgcaatgtg taaagtagca aatcagaatg attttacgac agcaacaaga
    11941 ggtaccaagg taccagaaaa aacaactgac tacacagcag atacaatgac cttggttccg
    12001 tagattattt cagactaatt tttttattcg ccaagcaaat cctgctcttc aggcacccta
    12061 gcagttctcg tttttcaata tcacttcctt ctttcaggac gaacttcgca tagcctcgaa
    12121 catcgatact gtcagcgctg actttctcct tgtttcctag caggaatttc tgtagtttgg
    12181 aaaatcgttc aacgtcagcc tttatctttt ctttaacttc tatttcattt aaatttatct
    12241 tttcaatcag ttcgttgaac tgtttaatta cttctttctc ttcggtatag ccacatttac
    12301 aatccacccc gcgcgcttta gtacatccat aatagacgta tcttgcaaca ccaccgttct
    12361 tgagtttttt gaatttctcg tctgccgata taccagatcc gcataatcca cagaacatca
    12421 ttttagtgaa ggcaaattct ttattttcta ttcgcaacac attacttttt acctgacttt
    12481 gaacgagatc aaataattct ttagtaacta tcgcttcatg ttttcctgta taccagtttc
    12541 ctgactttct tggatattca aaaacaccat agtagaaagt attgtctaaa atcttataga
    12601 tatttcccaa actcaaatgt ttctttcctg cggcggttct catattgagg tcaaatttga
    12661 gccagttgta tatctttcgt ccgctccagt gctcatacgc aactttctca aacattttct
    12721 taatgattgg ggcacgctct ggatcaagaa gtgtttcgca cttccgatcc gttcgctttt
    12781 ctttcatgta gccagttgga gcaggtccag gccaacgcca tcggcctgcg cgcctccctg
    12841 gccctg
//
LOCUS       DAOTEX010000018        12822 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3118
            NODE_3433_length_12822_cov_4.366962, whole genome shotgun sequence.
ACCESSION   DAOTEX010000018 DAOTEX010000000
VERSION     DAOTEX010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868142
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 12822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 883
            CDSs (total)                      :: 833
            Genes (coding)                    :: 829
            CDSs (with protein)               :: 829
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12822
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3433_length_12822_cov_4.366962"
                     /isolate="AMR_MDS_3118"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            228..815
                     /locus_tag="P5056_01745"
     CDS             228..815
                     /locus_tag="P5056_01745"
                     /inference="COORDINATES: protein motif:HMM:TIGR00172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HRY62470.1"
                     /translation="MKIVLGSQSEARKKVLLRAGYEFDVVVAGIDEKEIRDPDPEKLT
                     LALARAKAKAILPKIKEPSLLITSDQVVTFNGTMREKPENVGEAFQFLFTSHLHPSST
                     VTAVVVTNTKTGERFVGVDVATIWFRPFTLQVIMDYIKTGDPFRLAGGFDHEHPLLAP
                     FIKKIDGEKESILGLPISLTEKLLDYAIMRTCLEV"
     gene            818..1438
                     /locus_tag="P5056_01750"
     CDS             818..1438
                     /locus_tag="P5056_01750"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRY62471.1"
                     /translation="MNSEEIVDLVDKRNRVIGQKKRSELGPFDLRRAVHIILMTLSEE
                     IIFQVRGANENDLYENMYDTAASGHVHAGEETREAARRELREETGIDCVPGDLCYLTT
                     TGPVEMDGTFIDPVFRSFYLFVFLGNSHNLTPEEGKVAGFVRIDLDKFVALKNGDPET
                     ERFIPAMLGEPFKNLMRMARSELRQRYPKVFDPEPNIGDTDSDFDW"
     gene            1536..1928
                     /locus_tag="P5056_01755"
     CDS             1536..1928
                     /locus_tag="P5056_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62472.1"
                     /translation="MKKHSILIAIAAFGLLILILQTFFLYFDIYDHAWWSDILMHILG
                     GAWVAMIGEYWLFLRDSDSKFKGGFWLKLILIVSFVSLVGFFWEFYEFSLDQILLTTH
                     QQSLPDTMGDLFNDILGGAVVSCFLNKK"
     gene            complement(2073..2456)
                     /locus_tag="P5056_01760"
     CDS             complement(2073..2456)
                     /locus_tag="P5056_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62473.1"
                     /translation="MKENFNETDYYAPEQLREQNREEDTKRVFSAEDFEDLIGEYSRL
                     RKDGSNVADLAFEKIIEHVDDPELLIELLGQHSGVAGYAGPDTLEKRDRLLKKLKNLS
                     GLTEKQKSDISLYSPAQKEDLDDWN"
     gene            2511..3530
                     /locus_tag="P5056_01765"
     CDS             2511..3530
                     /locus_tag="P5056_01765"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HRY62474.1"
                     /translation="MFCRNGYLKKIKKGGISMGNINESIVLVGGGGGVYRVARFLKHI
                     RPNITTIQTTFDHGGYSGTLRDERGILPSGDIRQAILALSDDEVESELRELLSYRFTR
                     KNGSSLDSASVGNILLAALTEIKKGDLVMAINTLCRWFKVRGKVLPVSLNNAELCVSL
                     SDGSIVRGEGLIDKRAFSDNRRIVKAFLEPVAHIYFGAHDAIVGADKIVFCPGDFYTS
                     VIPNTLVDGFKEAIKESHAKIILVVNIMNKKSETGRFAVSNFTKILLKHIGRDKIDAV
                     VCNNGKLSRPLLASYRNEHSFPVKIDESATRKYTGNIIADNFVDEVGGIVRHKENIAS
                     IIASL"
     gene            3728..5536
                     /locus_tag="P5056_01770"
     CDS             3728..5536
                     /locus_tag="P5056_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62475.1"
                     /translation="MENFLSFLTNNINFLILADSVFLIVMFFAIVANMRQGFKHLDLT
                     PLTYYAFIKYGSVVLESYIYNFRQGLSIWVVVTYLMLNVFAGFFLFVFANRAKALGKT
                     TSLVTISVFFVVVLAVLYFYGLIYAVLIGYSFFVAVCYYLVAFRIFNVLWAGEEAPMG
                     YIAPSIFVVSVIESAVLILGFFYIFQGEFLNLLVVALFVRLIVIVVISSALFITYTNI
                     HRRMSITNAELLDMLYTDRIRLILLLTVLLAIIASFFLIRHFQEEDLNNFVADVDTHL
                     DTSAGVFRERVDLARGVVVSFARSSIFDNGMAHGPALAEEIDREAKTMKNIILYVMDT
                     SGNVIDSSNRDATDSFVGDNYSFRPYFIEALNGKAGEEHDYAAIGVTSKIAGYYLANP
                     IDDSRGTRIGVAVIKVNLFDIPGAEANSGGCYFISDNEHIVIYSKCKDKIFNTVLPVS
                     EDTIRKKIASEQYPYINNEYLASGLNKKYYDSEADIMYKDKESLVLLKQVDNLLFVGF
                     FSDKEKNTNLTFLTFLVAEIIAFFSLVLFFTRVFDHQTSESIAFRERDALLAKKEAES
                     EKKELERMNSFMIGREIRMVELKKQVKELEDKLRNK"
     gene            5662..6405
                     /gene="gpmA"
                     /locus_tag="P5056_01775"
     CDS             5662..6405
                     /gene="gpmA"
                     /locus_tag="P5056_01775"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011193964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HRY62476.1"
                     /translation="MSKLILLRHGESTWNKENLFTGWTDVPLSDRGVAESHEAAKLLK
                     GAGIKIDIAFTSVLSRATETLDIVLADLGINIPVFYSWRLNERHYGALQGLNKKETVE
                     KYGEGQVHIWRRGYSVRPPELKTGDSRDAINDPKYKDLARNEIPMAESLKDTEDRIMP
                     YWKEAILPEIKNGKNILFSGHGSGIRSIVRVLDGLDEKQVMDLNIPTGVPLIYEFDES
                     GLPIKHYYLGDEAVVAAKIEEVKAQTKIK"
     gene            6621..7379
                     /locus_tag="P5056_01780"
     CDS             6621..7379
                     /locus_tag="P5056_01780"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRY62477.1"
                     /translation="MEKTEGRQVIIMDLVLGPRKLVFIRHGESLRNKYGHTPFLPKEP
                     GAIQEILRRITDHGMPLTEMGLNQAMKTGVGLKKRFGVPDIIMYSDYLRAMQTTEAIL
                     SAYTKRERNRIVAYAEEACRERCLGPVHNMTLEESKAMFSDIGNLSRKMTEFSYRPPQ
                     GESLADTTERARAFLSLALKMGSGYETMFVVSHYRFIQCANWVLSGLTEYSFGQIFYS
                     DEMKLENCGIVVYKFDRDLGRMVMVEYGTTFYDE"
     gene            complement(7463..8044)
                     /locus_tag="P5056_01785"
     CDS             complement(7463..8044)
                     /locus_tag="P5056_01785"
                     /inference="COORDINATES: protein motif:HMM:NF024911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRY62478.1"
                     /translation="MGGPCAGKTTILSAIREEFADQTIFVPEVATLLLEGGFPIPGTH
                     LPWSEKWQSAFQSAVLPLQQALEDTNELVAEAKGRNLIICDRGLLDGAAYTPGGVAEF
                     CRRYNLNYEKALANYTAIIHLESLATSDPQKYGKRGNASRFETVERAREIELAVREAW
                     KGHPRYIFVGGKKDVSSKISEVVGMVRFLLSEK"
     gene            complement(8178..10352)
                     /gene="recG"
                     /locus_tag="P5056_01790"
     CDS             complement(8178..10352)
                     /gene="recG"
                     /locus_tag="P5056_01790"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HRY62479.1"
                     /translation="MAPQTLIEEIFRLNPIQKAGLKKIGLKTLEDLLMYLPSRYGEKA
                     VLKHIDETNDNEIAVVCGRVISTKIYKSFRTRMPMADMVVEDDTGRIKATWFHQPYMA
                     KKVEEGKFAKLTGTVTLKNGSRTMVNPDLEEVETMELAHHGSLFGKDETHQSPVYPET
                     RGVTSLWIKHAIDRALKNKIHELLEDVIPKEILAKYHLPSLQTALVWIHSPQKDSDAI
                     AARKRFAFEEVFLIQLARQKEKREYAKNPSFKITTSEDNIDIFTKNFGFPMTDAQNKA
                     IKQIVEDLRADKPMARLLEGDVGSGKTAVAATAAYSIISTSPYGKDYGNLGVAYMAPT
                     EILANQHFESFIKYFKHTGINVGLITGSGCKKFPSKINPEGWTDISRTQLLKWVANGE
                     IPILVGTHALIQKSVKFKHLGLAIIDEQHRFGVKQRLDLAKKTGDFGGGKKLPVPHLI
                     SMTATPIPRTLALTIYGDLDLTLLDEMPAGRKPIITEIVSPTHRKEVYEKIHKELEAG
                     RQAYIICPRIDEPDPDKEMAIIAKSVKEEAKRLKKDVFKEYNIDILHSKMTPAAKEKT
                     MKDFSDKKIDILVATSVVEVGVNVPNATVIVIEGGERFGLSQLHQLRGRVLRSNHQAH
                     CFIFTESKSPKTMERLKAIKTAKNGFELAELDLQLRGFGELSGKKQWGISDIGMEAIK
                     NLKMVEAARNEAKNILDDDIELKKYTMLKQKVDERTMGTHFE"
     gene            complement(10598..10942)
                     /locus_tag="P5056_01795"
     CDS             complement(10598..10942)
                     /locus_tag="P5056_01795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62480.1"
                     /translation="MEKVDDNKVLIHEVDGRQNGPVITLTTDPERVQMLWRKLKILTN
                     RLDHSRNSETQWNTVYEIEILDTLLNKGSVNTWDLSRKLADAYGSIDCRTFSFACGVI
                     QSYNDYGGTGLK"
     gene            complement(11352..11807)
                     /locus_tag="P5056_01800"
     CDS             complement(11352..11807)
                     /locus_tag="P5056_01800"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HRY62481.1"
                     /translation="MELLTKKNYINAIKKSIGVEMFQSIFAEKDGHETDITRKGQLSC
                     ALFVSSILKLFNLIDINKAPHSTVLSTLKNMEASGWKKVSENELEEGDVIVWEKIIDV
                     DGEEHEHIGFFIGNERAVSNSSEKRVPAEHHYTYDGKRGIISAWRAPNL"
     gene            complement(11844..12221)
                     /locus_tag="P5056_01805"
     CDS             complement(11844..12221)
                     /locus_tag="P5056_01805"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY62482.1"
                     /translation="MEQTNKIFIIEDEKFLREMLEDKFKAEKFNTDSVTTGKEALVYL
                     EKEKPALIILDLLLPDVNGFEILAKIRGEEKTKDIPVIVLTNYDQKEDIERCKKLGIF
                     GFMVKTSFSLNEIIAEARKALSA"
     gene            complement(12236..>12822)
                     /locus_tag="P5056_01810"
     CDS             complement(12236..>12822)
                     /locus_tag="P5056_01810"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRY62483.1"
                     /translation="FAGEQPKIISNMREVNARTIALVDDLFDVAKIEEGHFDYKFKND
                     NILNVVEKVFEEYKPQAEARGISFVFEKENIPEFSFDEHKMRLVVKNVVDNAIFYTKE
                     SGNVELRTKNEGGFAIISIKDNGIGIPEDQHDRIFSKLFRAPNALKIRPDGSGLGLFI
                     AKSIVESHGGTVELKTEENKGTEVVIKLPLRHTK"
BASE COUNT         3158 a         2692 c         3027 g         3945 t
ORIGIN      
        1 acaagtaagt gcaacactct gctaatctag agtgatatgc aatatagaca ttttttcaaa
       61 agcgtggtag tctttggtgg aaattttaag caccttgaaa ttggcccacc aaaattttct
      121 aaatatttag tagatgctcg ataaggctat attttattta gtcatcggct cagttagaga
      181 tataagttca taaggtgaaa aatttaggcg gacaagggag gtctcatatg aaaatcgtgc
      241 taggttctca gtccgaggcg cggaagaagg ttctcctgcg ggccggttac gaatttgatg
      301 tggtggtggc gggtatcgat gaaaaagaga tccgcgatcc ggaccctgag aagctgacgc
      361 ttgctcttgc tcgcgcgaag gccaaagcta ttttgccgaa gattaaggag ccgtcgcttc
      421 tcatcacgtc tgatcaggtc gtcactttta acggcacgat gcgcgaaaag ccggaaaatg
      481 tcggtgaagc gtttcagttc cttttcactt cgcatttgca cccgtcgagc accgttaccg
      541 cggtcgttgt aacgaatacc aaaaccggag agcggtttgt cggggtggac gtcgcgacaa
      601 tctggttccg tccgttcacg cttcaggtca ttatggatta cattaagacg ggcgacccgt
      661 tccggcttgc cggcggtttc gaccacgaac atccgcttct cgccccgttc attaagaaga
      721 ttgacgggga gaaggagagc attcttggtc tgcctatctc gcttaccgag aagcttcttg
      781 attacgcgat tatgcggaca tgtctggagg tgtgacaatg aatagcgaag agattgttga
      841 tcttgtagat aagcgaaacc gggtcatcgg gcaaaagaag cgtagcgagc tggggccgtt
      901 tgatcttcgc agggcggttc acattattct gatgactttg tccgaagaaa tcatttttca
      961 agttcgcggc gcgaatgaga acgatcttta cgagaacatg tatgacacgg ccgcgagcgg
     1021 acacgtgcac gccggtgaag aaacacgcga ggccgccagg cgcgaactcc gcgaagagac
     1081 cggaatcgac tgcgttcccg gagatctgtg ctatctcacg acgacggggc cggtcgagat
     1141 ggacggaact ttcatagatc cggtcttccg atctttttac ctctttgttt ttctgggaaa
     1201 tagccataat cttactcccg aagaggggaa ggttgctggt tttgtcagga tagaccttga
     1261 taagtttgtc gcattaaaga acggtgatcc ggagaccgaa aggttcatcc cggcaatgct
     1321 cggggaaccg tttaaaaatc tgatgcgcat ggcaagaagt gagctccggc agagataccc
     1381 aaaagttttt gacccggagc ctaacatcgg tgacactgac tctgattttg attggtagaa
     1441 aggaagatat tgtagatggc gttctagaaa tagagcgcct ttttctttcc cttcgaattt
     1501 ttctgtccgg agtgatataa tacaatgatt gttttatgaa aaaacattca attttaattg
     1561 caattgccgc attcggatta ttgatcttaa ttttgcaaac tttttttctt tatttcgata
     1621 tatatgacca tgcctggtgg tccgatatat taatgcatat tttgggtggg gcctgggtgg
     1681 caatgatcgg cgagtattgg ttatttttaa gagacagtga ctccaagttc aaaggtggtt
     1741 tttggttaaa attgattttg atagtcagtt tcgtctcgct cgttgggttt ttttgggagt
     1801 tttatgagtt ttctttagat caaattcttc tgacgaccca ccaacaaagt ttgcctgaca
     1861 cgatggggga cctttttaat gatattctgg gaggagctgt agtctcttgt tttttgaata
     1921 aaaaataggg aattattttt taattaaata attttcaaaa attatttaga aatattaata
     1981 ccgtattgcc gtgaacgtgg cagagaaata gcacgagtct tgtgcgggca agttaaaaaa
     2041 attttgaagt gtttccaaaa acaggaatcg tattaattcc agtcgtccaa atcttctttt
     2101 tgagccggag agtagagact aatatcagat ttttgttttt ccgtcagccc cgacagattt
     2161 ttaagcttct ttaataagcg atctcttttt tccagtgtgt cgggacccgc ataacccgcc
     2221 actccggagt gctggccaag tagttctatc aataattccg gatcatcgac atgctctatt
     2281 attttttcga aggcaagatc cgccacattg cttccgtctt tcctaagtct ggagtattcc
     2341 ccgataagat cctcaaaatc ttccgcagaa aaaacccgct tagtgtcttc ctccctgttt
     2401 tgctccctta attgttctgg agcgtaatag tctgtttcgt taaaattttc tttcatatta
     2461 agattctatc ttttacgtta cccattgtct atattttcag ttttagtagt atgttctgca
     2521 gaaacggtta tttaaaaaag ataaagaaag gtggtatctc tatgggtaat atcaatgaat
     2581 caatcgttct tgtgggcggc ggtggcggtg tataccgcgt cgctcgcttt ttgaagcata
     2641 tacggcccaa tataacgaca attcagacaa catttgacca cggcgggtat tccggcaccc
     2701 ttagggacga gcggggtatt ttgccctcgg gtgatatcag acaggcgatt ctggctcttt
     2761 ctgacgatga ggttgagtct gagcttcgcg agctcctctc ttataggttt acccgcaaga
     2821 acggttcctc gcttgattcc gcatctgtcg gcaatatttt gcttgcggcg cttaccgaga
     2881 tcaagaaagg ggacttggtt atggcaatca ataccctctg tcggtggttt aaggtcaggg
     2941 gcaaggtctt gccggtaagc ttaaataacg cggagctttg cgtttcgctt tccgatggtt
     3001 ctattgtgag gggagagggg cttatcgata agcgcgcctt ttccgacaac agaagaatcg
     3061 ttaaggcttt tcttgagccg gttgctcaca tttattttgg ggcgcacgat gccattgtcg
     3121 gcgccgacaa aatcgtcttt tgtccgggcg acttttatac ttcggttatt ccgaacactc
     3181 tggtcgacgg attcaaagag gccataaagg agagtcacgc aaagataatt ctcgttgtga
     3241 atattatgaa taagaagtcg gagacgggca gattcgctgt ttccaatttc accaagatac
     3301 ttttgaaaca tatcggtcgc gacaaaatag acgcggtcgt ctgtaacaac gggaagctga
     3361 gcaggcccct gcttgcttca tataggaacg aacattcatt cccggtaaag attgacgaaa
     3421 gtgccacgcg aaaatatacc ggaaatataa ttgcggacaa ttttgtcgat gaggtcggcg
     3481 gaatcgttcg ccataaggaa aatatcgcct ctattatcgc aagcctatag tacaccccct
     3541 ctcacgagga gggtgttttt tattgttccg tttcgcctgc cccgcaggag gctggtaaat
     3601 atggtaaaat tctaagagtg tgggtagccg aaggcgactt ccgtgccccg tcgtattagt
     3661 ttacggacat tttttctttg agccttcctg ccggggtgat acaatatagg agtaataaaa
     3721 taataatatg gaaaatttcc tatctttttt gactaataat attaattttt taatattggc
     3781 cgactctgta tttttgattg tgatgttttt cgccatcgtc gccaatatga gacagggatt
     3841 taagcatcta gatcttaccc ccctcacata ttatgcgttt ataaaatatg gctcagttgt
     3901 tctggagtca tacatatata acttcagaca aggtctatct atttgggttg tagtaacata
     3961 cttgatgtta aatgtgtttg cagggttttt cttatttgtc tttgcaaata gggccaaggc
     4021 cttaggcaag acgacatctc tagtaacaat atcggtgttt ttcgttgttg ttcttgctgt
     4081 cctatatttt tatggtttga tttacgcggt gcttatcgga tattcttttt ttgtcgctgt
     4141 ttgttattat ctggtggctt tccgaatttt taatgtttta tgggccggag aggaggcccc
     4201 tatgggatat atcgcacctt ctatatttgt agtttcggta attgagtcgg cggttttaat
     4261 tttaggtttt ttctatattt ttcagggaga attcttaaat ttgcttgttg tggcattgtt
     4321 tgttcgtctc atcgttatag tggtcatttc atcggcactt tttattactt atacgaacat
     4381 acatcgcaga atgtccataa caaatgccga attgctggat atgctttata cagacagaat
     4441 aaggctgata ttattattaa ccgtattgtt agcgataata gcctccttct ttttaattag
     4501 acattttcaa gaggaagacc tgaataattt tgtcgccgat gttgatacac acctggatac
     4561 atcagcaggg gtgttcagag aaagggtgga cctagcccgt ggggtggtgg tctctttcgc
     4621 gagatcgtca atattcgata acggtatggc gcatggcccg gccttggccg aagagataga
     4681 cagggaagcg aagacaatga aaaatattat tctgtatgta atggacacaa gcggaaatgt
     4741 aatagactca agcaacagag atgccacgga tagttttgtc ggggataatt actccttcag
     4801 accatatttt attgaggcgc tgaatggaaa ggcgggtgaa gaacacgact atgccgccat
     4861 cggtgttact tccaagattg ccggctatta tctcgcgaac ccgatagatg attccagggg
     4921 cacaagaatc ggtgtggcgg tgattaaggt taatttattt gacatcccgg gggcggaggc
     4981 gaacagcggc ggatgttatt ttatttccga taatgagcac atcgtaatat attctaagtg
     5041 taaggataag atttttaata cggtccttcc cgtaagtgag gataccattc gtaaaaagat
     5101 tgcaagcgag cagtatcctt atataaacaa tgaatatttg gctagcggtt tgaacaagaa
     5161 atactacgac agcgaagcag acattatgta taaagataaa gaaagtttag ttttgctgaa
     5221 gcaggtagac aatttgttgt ttgttggatt tttttctgat aaagaaaaaa ataccaattt
     5281 aacgttctta acttttttgg ttgcggagat aatcgctttt ttctctctgg tattgttttt
     5341 tacgagggta tttgatcacc aaacatcgga gtctatcgca tttagagagc gggacgcact
     5401 tttggcaaaa aaagaagctg agtcggagaa aaaagagctg gaaagaatga atagttttat
     5461 gatcggacga gagattagga tggtagagct taaaaaacag gtcaaggaac tggaagataa
     5521 gcttagaaat aaataataaa aaggcgcctc gtggggagcg ctttttagta agttttttat
     5581 ccccgcgcag atgggaatga caagcgggga aattaattaa ggatattcca cgagtttgtt
     5641 tgtttaatgt ataatattct catgtcaaaa ttaattttgc ttagacacgg agaaagcaca
     5701 tggaataaag agaatctttt tacagggtgg actgatgtgc ctctttcaga taggggggta
     5761 gcggaatctc atgaagcggc aaaattactc aagggcgcgg gaattaagat tgatatcgct
     5821 ttcacttctg ttctttcgcg agctacagag accctagata ttgttctcgc tgatttagga
     5881 attaacatac ctgtttttta ttcttggaga ttgaacgaaa gacattatgg cgctctgcag
     5941 ggcttaaata aaaaagaaac ggtggaaaaa tacggcgagg ggcaagtgca tatatggagg
     6001 agaggctatt cggtaagacc gcctgagttg aagacgggtg actccagaga tgcaattaac
     6061 gatccaaaat ataaagacct cgcaaggaac gaaataccga tggcggagtc gttgaaagac
     6121 acagaagaca gaataatgcc gtattggaaa gaggcaatct tgcctgaaat aaagaacggc
     6181 aagaatatac ttttttcagg ccacggttcc ggcataagaa gtatcgtgag ggtgcttgat
     6241 ggtttggacg aaaaacaggt tatggatctg aatattccga caggggttcc gcttatatac
     6301 gaatttgatg agtcggggtt acccatcaag cattattacc tcggggacga ggctgtcgtc
     6361 gcagccaaga tcgaagaggt caaggcgcag actaaaatta aataaattta ttaaaaaatg
     6421 ccgctccccg caggaaagtt caaataaatt atttttaaaa aacaaaataa ttcgctcggt
     6481 taccgcctgc ctgccctgcg tgcgcgggca cggcaggcgg ctttgttatt tgtaagtaag
     6541 gcgccaagag agaaacttcc tacggggtga acttgtaaag ggcggtcttt tgttttagta
     6601 tccaaggtga atgtaggact ttggaaaaaa cagaaggaag gcaggtaatc attatggatc
     6661 ttgttctggg ccccagaaaa ctggtcttta ttcgtcacgg tgaatcactg cgcaacaagt
     6721 atggccacac gccctttttg ccgaaagagc ccggcgctat tcaggagatc ttgcgtagaa
     6781 ttaccgacca tgggatgccc cttaccgaaa tgggtctcaa ccaggccatg aaaaccggcg
     6841 tggggctcaa aaagaggttt ggtgtgcccg atattataat gtattccgat tatttgaggg
     6901 caatgcagac gacggaggcg atactttctg cttatacgaa gagggagagg aatcgtatcg
     6961 tcgcatatgc cgaagaggct tgtcgagaga ggtgcctcgg ccccgtgcat aatatgaccc
     7021 ttgaagagtc aaaggcaatg ttcagcgata tcggcaatct cagtaggaag atgacggaat
     7081 tttcctatcg accaccgcaa ggcgaaagcc tggcagatac gacggagcgc gcaagggcgt
     7141 tcctttcgct cgcccttaag atgggctccg gttatgagac gatgttcgtt gtctcacact
     7201 atagattcat ccagtgcgca aattgggttt tgtccggtct gaccgaatat tctttcggcc
     7261 agatatttta ttcggatgaa atgaagctag agaattgcgg aattgtcgtc tacaagtttg
     7321 accgcgatct cggccgtatg gtcatggtcg aatacggcac cactttctat gacgaataag
     7381 tacatcgacc ccgcgcggaa gtgcggggtt tttattttgg tggcttaaat aaaaaagccc
     7441 ggactttcgt ccgagcattg tcctactttt cagagagaag aaatctgacc attccaacga
     7501 cctcgcttat cttgctggag acatccttct ttccaccaac gaagatatac cgagggtggc
     7561 ctttccacgc ttctctcacc gctagttcaa tctccctggc cctctcaact gtttcaaaac
     7621 gggaagcgtt ccctcttttt ccatattttt gggggtcaga ggttgcaaga ctctccagat
     7681 ggataatcgc agtgtagttc gctagagctt tctcatagtt gagattgtag cgtctgcaga
     7741 actccgccac tccgcccgga gtgtaggccg cgccgtccag gagtcctctg tcgcagatga
     7801 taagattacg ccccttcgcc tctgctacca gctcatttgt gtcttccagg gcctgctgaa
     7861 gaggtagcac ggcagattga aacgcagatt gccacttctc gctccacggg agatgcgtcc
     7921 ccggaatcgg gaagccgcct tcgagcagta gtgtcgcaac ttcggggaca aagatcgttt
     7981 ggtctgcgaa ctcttctcgg attgcactca gaatcgtggt ttttcccgca caggggccac
     8041 ccactagggc aattttcttg atacccattt tttcctccct tatgctctgc ctcccattaa
     8101 agatgagggc ttacgagttt tttcaaagta aaacagctct gcgatatagt atcgcatcat
     8161 atatttatgt caaatagtta ttcaaaatga gtccccatcg tcctttcgtc aactttttgt
     8221 ttaagcattg tatatttttt gagttcgata tcgtcatcca gaatattttt agcttcattt
     8281 cgcgccgcct ccaccatttt tagatttttt atcgcttcca ttcctatatc tgatataccc
     8341 cactgctttt tgcctgaaag ttctccaaaa ccgcgtagct gtaggtcgag ttcggctagt
     8401 tcaaatccgt tcttggctgt ttttattgct ttcagccgct ccatagtctt tggtgatttc
     8461 gattctgtga aaataaaaca atgcgcttga tgattgcttc gcaaaactct tccgcggagt
     8521 tggtggagtt gtgataaccc gaatctttct cctccttcga tgacaataac tgttgcgtta
     8581 gggacattta ctccgacctc gacaacagat gtggcgacca aaatatcgat tttcttgtcc
     8641 gaaaagtcct tcatagtttt ttctttcgcc gccggggtca tcttgctgtg aagaatgtcg
     8701 atattatatt ctttgaaaac atctttcttg agacgtttcg cttcttcttt cactgatttt
     8761 gcgattatcg ccatttcttt gtccggatcc ggctcgtcaa tgcgcggaca aattatgtaa
     8821 gcttgtcgtc cggcctctaa ttccttgtgt attttttcat agacctcttt tctgtgggta
     8881 ggggagacta tttctgtgat tatcggcttt ctgcccgccg gcatttcgtc gagtagtgtg
     8941 aggtcgaggt ctccgtatat agtgagagcg agggtccttg gaatcggtgt cgccgtcata
     9001 gaaattaggt gagggaccgg cagttttttg ccgccgccga aatcaccggt cttttttgcg
     9061 aggtccagtc tttgttttac gccgaatctg tgttgttcgt ctattattgc gaggccaaga
     9121 tgcttgaatt taacgctttt ttggatgagc gcgtgtgtgc cgacaagtat cggtatctcg
     9181 ccgtttgcga cccatttcag gagctgtgtg cgagaaatgt ccgtccaccc ctccgggttt
     9241 atttttgatg gaaatttttt acacccgctt cccgtaatta ggccgacatt tattcccgtg
     9301 tgcttaaaat attttatgaa agattcgaag tgctgattcg cgagtatctc tgtcggcgcc
     9361 atatatgcga caccgaggtt cccgtaatct tttccataag ggcttgtgct gattattgaa
     9421 tatgcggcgg tcgcggcgac ggccgtcttt ccggatccga cgtctccctc gagaagtctt
     9481 gccatcggct tatcggcgcg aaggtcttct actatctgct ttatcgcttt gttttgggcg
     9541 tccgtcatcg ggaacccgaa gttttttgta aaaatatcta tattgtcctc ggacgttgtt
     9601 attttgaacg aaggattttt cgcatactct cttttttctt tttgtcttgc gagctgaatg
     9661 aggaaaactt cttcaaaggc aaatcttttg cgtgcggcga tagcgtcgga atctttttgc
     9721 ggactatgta tccagacgag cgccgtctgt agagagggga gatgatattt cgcgagaatt
     9781 tctttaggaa ttacgtcttc taaaagctcg tggattttat ttttcagagc tctgtctatc
     9841 gcatgcttta tccaaagcga tgtgacccca cgcgtttccg gatagacagg cgattggtgc
     9901 gtttcatctt tgccgaatag acttccgtgg tgggcgagtt ccattgtttc aacctcttcg
     9961 agatcgggat ttaccatcgt gcgcgagccg ttcttcagcg tgacggttcc cgttagcttt
    10021 gcaaattttc cttcttccac ctttttcgcc atatatggct ggtggaacca tgtcgctttt
    10081 atccttcccg tgtcatcctc gacgaccata tcggccatcg gcatcctcgt gcggaacgat
    10141 ttgtatattt ttgtgcttat aacgcgtccg cagactacag ctatctcgtt gtcgttcgtt
    10201 tcgtctatgt gcttaagcac agctttttct ccgtatctgg acgggagata cattagtagg
    10261 tcttctaggg ttttgagccc aatctttttc agccccgctt tttggatcgg attaagacga
    10321 aatatttctt cgatgagtgt ctgtggggcc attaataaac aggataacct gtggacgtaa
    10381 aaattaaaag tagtcttttc tccgtcattg cgaacgcgaa gcgtgtggca atccaggaaa
    10441 agtagttgta aggttgaaat ttttaacgtt gtaaagtttt aaataaatct ggattgccgc
    10501 gtgctaaagc cctcgcaatg acgataaata aaatatggtt aggcaaattt aaaaggcaac
    10561 cattgctggt tgccttgttg gaggctttcg cctgtttcta cttgaggccg gtgccgccgt
    10621 agtcgttgta actctggatg actccgcagg cgaacgagaa cgttcggcag tcgatcgagc
    10681 cgtaggcgtc ggcgagcttc cgcgacaggt cccacgtgtt gaccgagccc ttgttgagga
    10741 gcgtgtccag gatctcgatc tcgtagaccg tgttccactg agtctcggaa ttacgggaat
    10801 ggtcgagacg gttcgtgagg atcttgagtt tccgccagag catctgcaca cgctcggggt
    10861 ccgtcgtgag cgtaatcacg gggccgttct gccggccgtc gacttcatgg atgaggacct
    10921 tgttgtcgtc cactttttcc atgagcggtt cctccttatt ctgccttgtc gaaagcaccc
    10981 gcgctttcga tgctgatggc gcttccgtcc ctcctgatga acccgcgtct cccgaacatt
    11041 cgcacgatcg gatgcaagat gatggccacg atggcccagg caaccgtgac gagcacaatg
    11101 agcgtgatgg cgatggggtt ggtctgttcc ttgcctctct ccacttgaaa gtccactctt
    11161 tgtcgaacat gtgcggtccc tccttcgttt tcggtgggtt tttagcccaa aattgtttag
    11221 gaactcgatt taaccctctt ttttaattat acaccttatg tgtataaaag tcaagccata
    11281 atgaaagata tcaatcaaaa caaagccact tggaaaccgg gtttccaagt ggcctgtatg
    11341 atgaagtctt tttatagatt tggggcgcgc caggcggaga tgatccctct tttgccgtca
    11401 taagtatagt ggtgttctgc gggaacgcgc ttttcagagc tattgcttac agctctctcg
    11461 ttaccgataa aaaatccgat atgctcgtgt tcttcgccgt cgacgtcgat aattttttcc
    11521 cagactatca cgtctccctc ctctaattca ttttccgaaa cttttttcca accgcttgcc
    11581 tccatatttt taagagtgct caatactgtg ctgtgcggcg ctttgttaat atcaatcagg
    11641 ttaaataatt ttaatatcga agaaacaaaa agagcgcagg agagttgtcc ttttcttgtt
    11701 atatctgtct cgtgaccgtc tttttcggcg aagatacttt gaaacatctc tacgcctata
    11761 gattttttga ttgcgtttat atagtttttc tttgttagaa gttccataac gagtagtata
    11821 ttactttgcg caaaataaaa aagctacgcg cttagcgctt ttctcgcttc agcgataatt
    11881 tcatttagag agaagcttgt tttcaccata aatccgaaga tgccgagctt tttgcacctc
    11941 tcgatgtctt ctttttggtc gtaatttgta agaacgatga ccggtatatc tttcgtcttt
    12001 tcttctccgc gaatttttgc gaggatctca aatccgttca cgtcggggag aaggaggtct
    12061 agtataatta gcgcaggttt ttctttttca agatacacga gcgcctcttt tcctgtagta
    12121 acgctgtcgg tattgaattt ctccgccttg aatttatcct caagcatctc gcgcaagaat
    12181 ttttcgtcct ctattatgaa aattttgttt gtttgttcca tttattttaa aaactctatt
    12241 tagtatggcg aagcggtagt tttattacaa cttcagttcc tttgttttct tccgtcttta
    12301 attctacggt tccgccatgg ctctccacaa tgctctttgc gatgaaaagt ccgagtccag
    12361 agccgtccgg ccttatttta agagcgttag gagcgcggaa gagctttgaa aagatcctat
    12421 cgtgctgatc ctcgggaata ccgataccgt tatcttttat tgatattatt gcaaaaccgc
    12481 cttcattctt cgtccttaac tcgacatttc cgctctcttt tgtgtaaaag atagcattgt
    12541 ctacaacatt tttcacaacg agcctcattt tgtgttcatc aaaagaaaat tcaggtatgt
    12601 tctctttttc aaaaacaaat gatatacctc tagcttcggc ttgtggctta tattcttcaa
    12661 aaactttttc gacaacgttc agtatgttgt cgtttttgaa tttgtagtcg aagtgaccct
    12721 cttctatttt tgcgacatca aaaagatcgt cgacgagggc tatcgttctc gcattcactt
    12781 cgcgcatgtt gcttattatt ttcggttgtt ctcctgcgaa ct
//
LOCUS       DAOTEX010000019        12385 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3118
            NODE_3582_length_12385_cov_4.463017, whole genome shotgun sequence.
ACCESSION   DAOTEX010000019 DAOTEX010000000
VERSION     DAOTEX010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868142
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 12385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 883
            CDSs (total)                      :: 833
            Genes (coding)                    :: 829
            CDSs (with protein)               :: 829
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12385
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3582_length_12385_cov_4.463017"
                     /isolate="AMR_MDS_3118"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1782
                     /gene="topA"
                     /locus_tag="P5056_01815"
     CDS             <1..1782
                     /gene="topA"
                     /locus_tag="P5056_01815"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HRY62484.1"
                     /translation="QEARRVLDRLVGYDLSGLIWKKVRYGLSAGRVQSPALRIIMEKE
                     REIRAFVPEGYWVIKAETETPKKEAIEFTYSEEPKTQEEADRVVALSKISNLEITGIE
                     ESDYTRKPKAPFTTSTLQQAASSGLGFSPSRTMKIAQKLYENGYITYMRTDSTTLSDD
                     AMSAIKAIVTKEYGEKAWTPREYKTKVKNAQEAHEAIRPAHVEKRTVAGGGEDSKLYD
                     LIWRRTVSSQMPDANFIRTKIVAETGDESVKPFHANGSRLINPGWLACDEYAKGDDTI
                     LPEVKKGEKLKITNIDSEGKQTEPPARYSEAGLVKELEKRGIGRPSTYAAIIQTIIAR
                     GYVEKDGKTLKPTDTGEVVSNFLEEYFANYISDNFTAQMEEDLDDIANGTKNYEKTLK
                     AFYGPFKKEVESKNKVPKVTTLGEAPKDIKCPECGSGMVIKLGRTGKFLSCDKFPECT
                     GARTITGEIMEGPKETGEDCPECKKGKLVERSGRFGKFIACNNYPKCKYIKKDEAAEA
                     EKLEKMKTGVKCPECENGDIAERKGRFGAFYSCTNYPKCKFIMKSKPTGNICDKCGAL
                     MMEGTKTIPERCSRKECENNRPERKAK"
     gene            1995..2387
                     /locus_tag="P5056_01820"
     CDS             1995..2387
                     /locus_tag="P5056_01820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62485.1"
                     /translation="MRKLLYASGELGHLRREHRALGTLGGKLAFAFTESLRQFHDLRT
                     VIVDDLVLHDDNLDGRLLGSPASIFLEEFVQLIEEFAESITFAHFSLFLSRALGARYY
                     YFKRPNSLKRAIKNAPLVEMATARIVPL"
     gene            complement(2384..2521)
                     /locus_tag="P5056_01825"
     CDS             complement(2384..2521)
                     /locus_tag="P5056_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62486.1"
                     /translation="MIAMINCLTFALAVMVATIFVVGLVYIFARFFCWAKSERFWEDL
                     F"
     gene            complement(2543..2974)
                     /locus_tag="P5056_01830"
     CDS             complement(2543..2974)
                     /locus_tag="P5056_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62487.1"
                     /translation="MKNPSIILRCSIEDKPCVFTQAGLTVVGPETGDQLPSLKRIFAS
                     GEVVMHHEYLQLKKGWFSTFNLSVQLLAGKNFDFKEYLPYLKQLVKTRYSYYALDVWF
                     DIENDVLWTTGSVLGAATLLQDLCNTKENWEKKKRPKKFID"
     gene            3292..4806
                     /locus_tag="P5056_01835"
     CDS             3292..4806
                     /locus_tag="P5056_01835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HRY62488.1"
                     /translation="MLEEPKKHHADTSEKDLREIFDLMYKYSDKDAALITKAYKFAEK
                     AHENYDRLSGEPYLVHLVGTAKNLAEIRADAETVAAGLLHDVLEDTPTKKEDVIREFG
                     EDIAFMIDGVTKLGKIKYHGLERHAESLRKLFVATAKDIRVLLIKLADRLNNAATLES
                     LYKIRPEKARRIALETIEIYAPLANRLGMGHIKGELEDLAFPYILPAEYKETVRLRQK
                     KSKEFMQRLEKINRLLCKRLAKAGITKFKTDFRVKHIYSLFKKLQKNNMDIEKIYDLS
                     ALRIIVPTTEDCYRVLGIIHNTWRPLPERLKDYIAVPKPNGYQSIHTVIFTGDGGIVE
                     IQIRNDQMHDEAEYGIASHLAYDESGKRKDKGAVLSKKLKWIGELIEWQKSVHESENF
                     LDNLKTDFFNNQIFVFTPKGDVVELPEGAGPLDFAYQIHTDIGNHAFGAKVNGKMASF
                     DTALKNSDIVEIITRDSSTPSPKWLEYAKTSEAIKRIRICLTNKKKKNERMEKK"
     gene            complement(4975..5325)
                     /locus_tag="P5056_01840"
     CDS             complement(4975..5325)
                     /locus_tag="P5056_01840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62489.1"
                     /translation="MSKKTNSKCIFKQELDDYFNDKSVRLRVNRSPEEISNMKEALQG
                     AVFMKILATEARKLERNRPKGNKHQEALEKIIRDLSLEVSSLDDRGAGNDLYEMARRI
                     EKTVRSLRKLQKLL"
     gene            complement(5363..5563)
                     /locus_tag="P5056_01845"
     CDS             complement(5363..5563)
                     /locus_tag="P5056_01845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62490.1"
                     /translation="MHDFDVKIGQKKEHLQKGEMVVAKKERSRDKSKKKRGPKRTKSQ
                     KSAENFIAYLKKSGLVDGFGEY"
     gene            complement(5702..6685)
                     /locus_tag="P5056_01850"
     CDS             complement(5702..6685)
                     /locus_tag="P5056_01850"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HRY62491.1"
                     /translation="MNDQIESKNNISNSIFWIETDKIVPNPYQPRKEFDEKALRELAD
                     SIKQYGILQPLVATRREIEKEDGGLAVEYELISGERRLRASRLAGLSQVPVVIRTGEQ
                     SDTLKFELAIIENLQREDLNVIDRAKAFQALADKFNYKHAEIAVKVGKSREYVSNSIR
                     VLALSPEIQDALINKKITEGHCRPLLMLGNKPEEQITLFKEIIYKKLTVREAESISRH
                     IATDKVRKKEYMMDPDILDMESKLTEKLGTRVHIEKKDFGGKVTIDFFSKDDLRSLLD
                     MVSKGKIVIPKNTTVAITETSAVSEAGTPVDDSTPDEVDDSELYSIKNFSL"
     gene            6963..7367
                     /locus_tag="P5056_01855"
     CDS             6963..7367
                     /locus_tag="P5056_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62492.1"
                     /translation="MFNFWKRNPEKELPKKAEEAATKRERRDRLDRASRIVHFPPEVP
                     GVGKTVQVAHYDEYDDIIVLEGTIMSRNRDFEYIDDDPTPKMSKSIVVTLSYFSESED
                     QQEQGFLDLFFDTETSEWSLTNDEEAIMTILD"
     gene            7383..7913
                     /locus_tag="P5056_01860"
     CDS             7383..7913
                     /locus_tag="P5056_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62493.1"
                     /translation="MKKIITLIILFIFFAGVMGLALRQRRSLPPTINFAMQPFSEPKN
                     DTEILTNLVYLYGSPMKGERDRKTLKLGDYAVAVSATPGEKPYLSIKYYKKDMTKDES
                     YEFEYKDFSLNGSVDEFHSAKRTYIIEGEIVIDFFPIIKFWHRGGVREGVYEDPQKDY
                     DGVLKEMVSLAKQKYE"
     gene            8004..8087
                     /locus_tag="P5056_01865"
     tRNA            8004..8087
                     /locus_tag="P5056_01865"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8038..8040,aa:Leu,seq:caa)
     gene            complement(8408..8989)
                     /gene="cas2"
                     /locus_tag="P5056_01870"
     CDS             complement(8408..8989)
                     /gene="cas2"
                     /locus_tag="P5056_01870"
                     /inference="COORDINATES: protein motif:HMM:TIGR01573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas2"
                     /protein_id="HRY62494.1"
                     /translation="MEKMETEVKKQIRMTKIQNGILTAVYAAGMISVALLAPNVLRVI
                     QSVSKKSGLYDKVTRGKKQSINVSRKRLIEAGLLIYKDGFLRLTKRGEDKMREFEARN
                     FELEKPRRWDGKWRVLIFDIKESRKYVRDKVRYTLRSVGFIRLQNSVWVYPYDCEDLV
                     TMFKADMKIGKDLLYIIADKIENDNFAREHFKL"
     gene            9208..9378
                     /locus_tag="P5056_01875"
     CDS             9208..9378
                     /locus_tag="P5056_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62495.1"
                     /translation="MALQYVRIVKFSEYPTRYRAYLSSNPKVWAEGSTPDDALGRLVK
                     LNPNIFHIRISE"
     gene            9857..10174
                     /locus_tag="P5056_01880"
     CDS             9857..10174
                     /locus_tag="P5056_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62496.1"
                     /translation="MRNFTILLLLVIISMLFMGCGEDDFLKYKLPKGQKLVLVFYPDD
                     LAENSLTIFYFCHVRGGQLITFNTPGNIIVYILTRNAEGGEKPATTQYSTGIVGTITI
                     TEQ"
     gene            10230..10712
                     /locus_tag="P5056_01885"
     CDS             10230..10712
                     /locus_tag="P5056_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62497.1"
                     /translation="MFYLTNDQSLPNANVTTLTSATPKKKAPVVDVLGAAVKNERPIT
                     DGTEGYYLGEVLAVLFDYAPLSPRGNDGVQNLITFLCRHKCPDLRLGDLETINRLKFH
                     AKTRLEIQFPALTKLKDEFEKMKEDSRKLKSPDNFMKSWVKKQTKIYGTTYFVRTVIK
                     "
     gene            10901..>12385
                     /locus_tag="P5056_01890"
     CDS             10901..>12385
                     /locus_tag="P5056_01890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62498.1"
                     /translation="MKKNLGYFLSTLLLALILSPLARAQEPVAAPTGLPAQSDPSDAD
                     KAQIEGEANSRAESETAAARAQFENDFGAPPEAFAKHGPKELPKYEEPKGIPESVRQY
                     VTDAEIVPIYCATVKWKTGEFFTSMNALKNNMLPAMQKVKSDLGISMDAPDIDGIISE
                     GQSRVDAICSAKTIDDADKLARDFQTWGLDPNRVPIESFMQEFAKKMQEKGDELRKQI
                     EAAIQPTINEETPKIQAEIEAEAKSMAGSFKFTKQPSAADIANMRAQIEERLKPIIEQ
                     KKEQVKQKVMAKIEEIKNGEAGKFQEYGKLFEGTEQKIETERKAGLSAYEKYKEEAFR
                     LRKEVVFNILDKNLAEGMKQLDAAEKDIEEAGKSDPSIRSVAEIKADIEADKKVLSSK
                     LDAALEAGDDGAFESALTDFRTKWEQVRKEGESLAKQSASKACNMALPQFENAKSQMD
                     GNMSKITSLEAKCAGSTSEECLTINEFSGRFATLKSKINDIKSAM"
BASE COUNT         3731 a         2942 c         2708 g         3004 t
ORIGIN      
        1 caggaagcta gacgcgtgct agataggctt gtcggctacg acctttccgg actcatctgg
       61 aagaaggtcc gctatgggct atcagccggc cgtgttcaat cccctgcact ccgcataata
      121 atggagaagg agcgcgagat ccgcgcattc gttccggagg gatactgggt tatcaaagca
      181 gagactgaga ctcccaaaaa ggaggcgatc gaatttacat attcagaaga accaaagacg
      241 caagaagagg ccgaccgcgt tgtggcgctt tcaaaaatct ctaatcttga aataacgggt
      301 attgaggaaa gcgactacac caggaagcca aaggctccgt tcacgacatc tacacttcag
      361 caagccgcaa gctccggcct cggattctcc ccttcgcgca cgatgaagat cgcccagaaa
      421 ctctacgaga acggatacat tacatatatg aggaccgaca gcaccacgct ttcagatgat
      481 gccatgtccg ccataaaagc aatcgtcaca aaagaatacg gagaaaaagc ctggactccg
      541 agagaatata aaacaaaagt aaagaacgcg caggaggcgc acgaagccat aaggcccgcc
      601 cacgttgaaa aaagaaccgt cgccggaggc ggagaagaca gcaagctcta cgatctcatc
      661 tggaggagaa ctgtctcaag ccagatgcct gacgcaaatt tcataagaac aaagattgtc
      721 gccgaaacag gagatgaatc cgtaaaacca tttcacgcga acggctcccg tctcataaac
      781 ccgggatggc tcgcctgcga cgaatacgca aaaggagacg acaccattct tcctgaagtg
      841 aagaaaggag aaaaattaaa gatcacaaac atagactccg aaggcaagca aaccgaaccg
      901 cctgcgagat attcagaggc cggacttgtt aaggagctcg aaaagagagg catcggtagg
      961 cctagcacct atgcggcaat catacagaca atcatcgcgc gaggctacgt agaaaaagac
     1021 ggcaagaccc tcaagccgac agacacagga gaagtcgtca gcaacttcct cgaggaatat
     1081 tttgcaaatt atattagcga caacttcacc gcacagatgg aggaggatct ggatgatatt
     1141 gcgaacggca caaaaaacta cgagaaaaca ctaaaggctt tttatggccc gttcaaaaaa
     1201 gaagttgaat caaaaaataa agttccgaaa gtaacaacgc taggagaggc gccaaaggat
     1261 attaaatgtc cggagtgcgg tagcggaatg gtaatcaaat tgggcagaac aggaaaattc
     1321 ttgagctgtg acaaattccc ggaatgcacc ggcgcaagga caataacggg agaaataatg
     1381 gaagggccaa aagaaaccgg cgaagactgc ccggagtgca aaaagggcaa gcttgtagag
     1441 agaagcggaa gattcggcaa gttcatcgcc tgcaacaatt acccgaaatg caagtatatt
     1501 aaaaaagatg aggccgccga agcggagaaa cttgaaaaga tgaagaccgg ggtgaagtgt
     1561 cctgaatgcg agaatggcga catcgcggaa agaaaaggga ggtttggcgc attttatagt
     1621 tgcacaaact acccgaagtg taagttcata atgaaatcaa agccaaccgg aaatatttgc
     1681 gacaaatgcg gcgcacttat gatggaaggc acaaaaacca ttcccgaaag atgttcccga
     1741 aaagaatgcg agaataatag gccggagagg aaggctaaat agtcccaagc ccataaagtc
     1801 aaaagccgtc tttctcaaac tccaaatatc atccccgcgt aggcagggaa ccatgtatta
     1861 ccttagattc ccaatcaggt cgggaatgac atcttcgagc aaagaaaaac cgccgactcg
     1921 acattgctgt cgagcggcgg ctgttacgac ttctgcttga gacattccac ttcggcattg
     1981 gcggcatcac gctgatgcgt aagctcctct acgcgagcgg tgagctcggt cacctgcgcc
     2041 gagagcatcg tgcactcggc acgcttggtg gcaagctggc cttcgcgttc accgagagtc
     2101 ttcgccaatt ccatgatctt cgcacggtaa ttgttgatga tctcgtcctt catgacgata
     2161 atctcgatgg cagacttctc ggttcccctg cctctatttt ccttgaagaa ttcgttcaac
     2221 tgatcgagga gttcgctgaa agcatcacct ttgctcattt ctcacttttc ctttctcgcg
     2281 cgcttggcgc cagatattat tatttcaaga ggccaaactc attaaaaaga gcaataaaga
     2341 atgcgccact cgtagagatg gcgacagccc gaatcgttcc tctctagaag agatcctccc
     2401 agaaacgttc tgattttgcc cagcaaaaga agcgggcgaa aatataaacc agcccgacaa
     2461 cgaagatagt ggcgaccatt actgcgagag caaacgttaa gcaattgatc attgcaatca
     2521 tccctttctt acgcgcttgg cgctagtcga tgaatttctt tgggcgcttc ttcttttccc
     2581 aattctcttt ggtgttgcaa aggtcttgta aaagtgtagc cgcgccgagg actgagccgg
     2641 tggtccagag aacatcgttc tcaatatcaa accacacgtc aagggcatag tagctgtatc
     2701 tggttttcac taactgcttc agatacggca aatattcctt aaaatcaaaa ttctttcctg
     2761 caagcaattg aacactgaga ttaaaagtgg aaaaccatcc cttttttaac tgcagatatt
     2821 cgtgatgcat gacaacctct ccgcttgcga agattcgctt gagcgaagga agctggtcgc
     2881 ccgtttccgg accaacaaca gtcagtcccg cctgagtaaa tacgcaaggc ttatcttcaa
     2941 tactacagcg caaaatgata cttggatttt tcatatcaga acctcccttt cgtgtattat
     3001 ctttctactt taataaattg acaaggtgcc ttaaattcac acaaaatcta taatttattg
     3061 acacctttgt caatgcatca attatatgtt aatttataac actcaaggga cttctgcttt
     3121 ctttttagtg ttataatcga aggagtatat cattgtcttg tcattccgag cttaccgagg
     3181 aatcgcttat acactaattg agcaagagat ctctcaacaa gccgtctaca ggtgccgatg
     3241 ttagcttcct tcggcctcga ggtgacaaag gaaaattaaa tctgtcaata catgctagaa
     3301 gaacccaaaa aacatcacgc cgacacaagc gaaaaagatc ttcgtgagat ttttgatttg
     3361 atgtataaat attcagacaa agatgccgcg ctcataacaa aagcatacaa attcgcagag
     3421 aaggcccacg aaaattacga caggttgtcc ggtgagccat atcttgtgca ccttgtcggc
     3481 accgcaaaaa atctcgcgga gataagagcc gatgcggaga cggtcgcggc cgggcttctt
     3541 cacgacgtcc tcgaggacac accgacaaaa aaagaagacg tcatccgcga gttcggagaa
     3601 gatatcgcct ttatgataga cggcgtcacg aagctgggga agataaaata ccacggcctc
     3661 gagcgacacg ccgaaagcct tcgaaaactt ttcgtagcga cagccaaaga tattcgcgtg
     3721 cttctcatca agcttgcgga cagattaaac aatgccgcca cgctcgaaag cctctacaag
     3781 ataaggcctg aaaaagcgcg aaggatagcg cttgagacga tcgagatata cgcccctctc
     3841 gcgaacaggc tcggaatggg acatatcaaa ggagagcttg aagacctcgc cttcccatat
     3901 attttgcctg ctgaatacaa agagacggtc cgcttgaggc aaaagaagtc caaagagttt
     3961 atgcagaggc tcgaaaaaat aaatcgcctt ctctgcaaga gactcgcaaa agccggaata
     4021 acaaaattca agaccgactt ccgtgtgaag cacatatatt ccttgttcaa aaaacttcag
     4081 aagaacaata tggacatcga gaagatctac gacctttctg cgctccgtat cattgtcccg
     4141 acgaccgaag actgctacag ggtgctcggt ataattcaca atacatggag acctcttcct
     4201 gagcgcctaa aagattatat cgcggtacca aaaccgaacg gctatcagag cattcacaca
     4261 gtcatcttca cgggtgacgg cggtattgtc gagatacaga taaggaacga tcagatgcac
     4321 gacgaggccg aatacggtat cgcctctcac cttgcctacg atgagtccgg caaaagaaaa
     4381 gataaaggcg ccgtgcttag caaaaagctg aaatggatcg gtgaacttat cgagtggcaa
     4441 aagagcgtcc atgaatcaga gaatttcctg gacaacctaa agaccgactt cttcaataat
     4501 cagatattcg tattcacccc caagggtgat gtggtggagc ttccggaagg tgccggccca
     4561 ctcgatttcg cataccaaat acacacagac atcggcaacc acgctttcgg cgcgaaagtg
     4621 aacggcaaga tggcatcatt cgacaccgca ctcaaaaata gcgacatcgt tgaaataatc
     4681 acacgcgatt cgagcacccc aagtccaaaa tggctcgaat atgcaaaaac atcggaggca
     4741 atcaagcgca tcagaatatg tctgacaaat aaaaaaaaga aaaacgagag aatggaaaaa
     4801 aaataatgaa tttggaactt actacaatat catttcgatt gggctgatat tgtagtaaaa
     4861 aataaacgta actactcaga tctaaaaaac ataatacacc cccccctttt tttgccacat
     4921 aaaataatat ttataaaaat ataaaagagc accgtgtggt gctcttgctc tgttttacag
     4981 aagcttctgc aatttcctca aagaacgaac ggtcttctcg attcgccgcg ccatctcata
     5041 aagatcgtta cccgcaccgc ggtcatcaag actagagacc tcgagagaaa ggtccctaat
     5101 gatcttctcc agcgcctcct ggtgcttgtt tcctttcggc cggtttcttt ccagcttgcg
     5161 cgcttcggtc gcaagaatct tcatgaaaac cgcgccctga agagcttcct tcatattgga
     5221 aatttcttcg ggagacctat tcacgcgaag acgaacagat ttgtcgttga aatagtcatc
     5281 caactcttgt ttgaagatac acttagagtt cgttttcttg ctcatgactc ctccttgtct
     5341 aaattaaaga gaaactagct atctaatatt ctccaaagcc gtcaacaaga ccggacttct
     5401 tcagataggc aatgaaattt tccgcggact tttgggactt ggtcctcttc ggacctcgct
     5461 tcttcttgga cttatccctg cttctttctt tcttggccac gaccatctcc cctttctgta
     5521 aatgttcttt tttctgtcct atttttacat caaaatcgtg caaaagtaaa accccacaaa
     5581 tgatacgggg tttgtgtttg tcattcccgc gaaggcggga atccatcttt tatatctcag
     5641 cttccataga ttcccgcctt cgcgggaatg acagctaaag attttgttgc aatctttatc
     5701 tttatagtga aaagttcttt atcgaatata actccgaatc atctacttcg tccggcgtgc
     5761 tgtcatcaac aggcgtcccc gcttccgaga cggcggacgt ttcggttatg gcaactgttg
     5821 tgttctttgg aataactatc ttcccttttg agaccatatc aagcagactc ctcaggtcat
     5881 ccttcgagaa gaaatctatc gtcaccttgc ctccaaaatc tttcttttca atatgaacac
     5941 gcgttccgag cttctctgtg agcttcgact ccatatcaag aatatccggg tccatcatat
     6001 attctttctt cctgacctta tctgtcgcaa tgtgccttga gattgattcc gcctccctca
     6061 cagtgagctt cttgtaaata atttccttaa aaagagttat ttgttcttcc ggtttattgc
     6121 cgagcatgag aagcggtctg caatggcctt ctgtgatttt cttgtttata agcgcatctt
     6181 gaatttccgg tgataatgcg agcactctga tgctgtttga aacatactca cggctcttgc
     6241 ccaccttcac tgcaatctct gcatgtttat agttaaattt atcggcgagc gcctgaaatg
     6301 ccttggccct gtcgataacg ttcaagtcct ctctctgtaa gttctcgatt atcgcaagct
     6361 cgaatttaag tgtgtccgac tgttctccgg tcctaataac cacaggcacc tgtgaaagtc
     6421 ctgcgagtct ggatgcgcga agccttctct ctccggaaat aagctcatat tcgacagcga
     6481 ggccaccgtc ttctttttct atttcacgcc ttgtagcaac aagtggctga agtattccgt
     6541 attgtttgat agaatcggca agctcgcgga gcgctttttc atcaaactct tttcggggtt
     6601 gataggggtt cggaacgatc ttgtccgtct cgatccaaaa tatcgaattg cttatgttat
     6661 tcttgctttc tatttggtcg ttcattgttt atgaaaatat attaactgaa ttataaaaaa
     6721 tagaagataa agaacagttt gtatctactc tctaccttct atcccctact ttctaatagc
     6781 ttgattttag caccacttcg cgcttttgca aaaagcgaaa aagtagaaaa acctgtgcaa
     6841 aatttgtggg aaaatatttg aattgacacc acgttcaaaa agaggtagtg tcttgaatca
     6901 ggtacgctca tttacacaat aataacgagc cacaggctcc caagaaacaa ggagggataa
     6961 ctatgttcaa tttctggaag agaaaccccg aaaaagagct gcccaaaaag gcagaagagg
     7021 ctgcgacaaa aagggaaaga agagaccgac tcgatcgcgc atccagaatc gttcatttcc
     7081 ccccggaggt tcccggggtc ggcaaaaccg tccaggttgc gcattacgac gaatacgacg
     7141 acatcatcgt cctcgaagga acaattatga gcagaaaccg tgatttcgaa tatattgatg
     7201 acgaccccac ccccaaaatg tcgaaatcta ttgtggtaac cttgtcttat ttctcggaat
     7261 cggaagacca acaagaacaa ggttttctcg atctcttttt cgacacggaa acctctgaat
     7321 ggtcgcttac caacgatgag gaagccatca tgaccattct cgactaaaag gaggtcgaaa
     7381 aaatgaaaaa aattatcacc ctgataatct tatttatatt tttcgccggg gtcatgggat
     7441 tagcgctgcg ccaacgcagg tcactcccgc cgaccattaa cttcgcaatg caacccttct
     7501 ccgaaccaaa gaatgacacc gagatcctca cgaatctcgt atatctttat ggatcaccta
     7561 tgaagggtga acgagatcgc aagactctca aactcgggga ctatgctgtc gccgtttcgg
     7621 ccaccccggg agagaaaccg tatctctcca tcaaatatta caaaaaagac atgaccaagg
     7681 acgaatctta tgaattcgaa tataaggact tttcgttaaa cggtagtgtc gacgaatttc
     7741 atagtgccaa gcgaacgtat ataattgaag gggaaattgt tattgatttc ttcccaatca
     7801 tcaaattttg gcacagaggt ggagtcagag aaggtgttta cgaagacccc caaaaagact
     7861 acgatggtgt tctaaaagag atggtctcgc tcgccaaaca gaaatacgaa taagagaggc
     7921 tttcaccgcc accctacccg ggtggctttt ttatttttgc aataaatggg gttttcttgt
     7981 atattactta aacaattgaa tatgccgcgg tggcggaatt ggtagacgca atagactcaa
     8041 aatctatcga tcgcaaggtc atgagagttc gattctctcc cgcggcacaa aataaccaca
     8101 caaacgcgaa agcgtttgtg tggttttatt ttggcctagc gggagagaat cgaaggccgg
     8161 aagcgggccc ctttctgaaa gaaagggagc tgaggtgggg tcgcgggtcc gtcgaagacg
     8221 gaacctgtga ccgattctct cctttaaaca tttgggcggt tttattttgg cctagcggga
     8281 gagaatcgaa ggccggagcg agattttttg ttcttgctct aaaatatttt ctatccacca
     8341 ccatacacaa ataatttcgc ggtcgcgaaa ttatttgtgt atacaatacc aacgaaaaag
     8401 agtcccacta aagcttaaag tgttccctcg caaaattatc gttctctatt ttatccgcga
     8461 taatatacaa aagatctttg cctatcttca tatcagcctt aaacattgta accaaatctt
     8521 cacagtcata tggatacacc caaacactat tctgtagtct tataaaacca acggaacgca
     8581 gtgtgtatct tactttatcc cgaacatatt ttcttgattc tttaatatca aatatcaaca
     8641 ccctccattt tccgtcccat cttctgggtt tttctaactc aaaatttctc gcctcaaatt
     8701 cgcgcatttt atcctctccc cttttggtca atcggagaaa accatctttg taaattaaaa
     8761 gccccgcctc aatgagtctt tttctagaga cattaattga ttgtttcttt cctctggtaa
     8821 ctttgtcata aagtcctgac tttttactca ctgattgtat tactctcaaa acgtttggtg
     8881 ccaaaagcgc gacagaaatc atccccgcgg catacacagc tgttagaatg ccattttgta
     8941 tttttgtcat tctaatctgc tttttaactt cagtttccat tttttccata atcgaaaatt
     9001 ttaacacaaa aacccacata cacaaataat ttcgcggtcg cgaaattatt tgtgtataag
     9061 atagaatctg tattttaata ttgcatattg cgttagttac ttgaatctaa gctattggat
     9121 cataacggct gtattgactc tgaccttaaa atatgttaaa caggaggctg aaaatcgaac
     9181 cttaacaatc aaattaagga ggagaaagtg gcacttcagt atgtgagaat cgttaagttt
     9241 tctgagtatc cgacaaggta tcgggcctat ctttccagta atccaaaggt gtgggccgag
     9301 ggaagcactc ccgatgacgc ccttggccgc ctggtcaaat tgaacccgaa catctttcac
     9361 attcgtattt cagaataaaa ggagtgaact cacaatgacg accgccctgc caattctcct
     9421 ccttattaaa ttagcatttc aggtttccgc ctccgccgcg tgctttaaat tagcccacga
     9481 catttacaaa gacggaaaca agggcttctg gcatttctgg tttgccggaa gcgggttcgc
     9541 gctgttctgt ctttttgtgg tcgacgccca agagctactt gtgcgactcg gattgcaata
     9601 aattacaaaa gcaaacagtc atcgacctcc gcgagctcgg attctagctc accccccggc
     9661 gaaagtcggg gttttattta tctattatgt aaaaatatca aggtcggacc ttgatattcc
     9721 cttatttatg gccgttttgt cttattttgc ttgacttttt acccaaaagg tgtataattg
     9781 taggacaagg ttagttcact gaaaacttaa taaaaacaag ccaatttggc tcaaaacacc
     9841 tgaaagaagg tagaaaatgc gtaatttcac gatactcctt ctcttggtta tcatttccat
     9901 gttattcatg ggttgcggtg aggacgattt tctgaagtac aagttgccca agggtcaaaa
     9961 acttgtcttg gttttttacc cagacgattt ggcagaaaac tctctcacca tcttttactt
    10021 ttgccacgta agaggtggtc agctgataac tttcaacacc ccaggcaaca tcatcgtgta
    10081 catactgacc aggaacgcag aaggaggaga aaagccagca acaacacaat atagtacagg
    10141 tattgtagga actattacaa tcaccgaaca gtaagaaggc tcaccagtac agccgcaagg
    10201 ctcagtcgcc tctcctgaaa ggagaaaaca tgttctatct cacaaacgac cagtccctcc
    10261 cgaacgcgaa cgtcacgacc ctcacctcgg ccacgccgaa gaagaaggcg cccgtcgtcg
    10321 acgtgctcgg cgccgcggtg aagaacgagc ggcccatcac cgacgggacg gagggatact
    10381 acctcggcga ggtcctcgcg gtcctcttcg actacgcccc cctctcccct cgcgggaacg
    10441 acggcgtgca gaatctcatc acgttcctct gccggcacaa atgtcccgac ctgcgcctcg
    10501 gcgacctcga gacgatcaac cgcctcaagt tccacgccaa gacccgtctc gagatccagt
    10561 tccccgcgct cacgaagctc aaggacgagt tcgagaagat gaaggaggac tcgcgcaagc
    10621 tgaagagccc cgacaacttc atgaagtcgt gggtcaagaa gcagaccaag atctacggca
    10681 cgacatactt cgtccgcacg gtcatcaagt agagacgaaa gaaggcttca cgcggaagta
    10741 aaacgcactc gccccgcaag gattcaatcc ttcgcggggc tttttgttta caaaaaatta
    10801 aaaaaccgga ttcccgtatt cacgggaatg acacaaactg acacaagcgg tataattaac
    10861 aacgtatatt aaaaaaatta gcagcgtaaa ttatattctt atgaaaaaga atttaggtta
    10921 ttttttatca acacttcttc tcgcactcat actctcccct ttggccagag cgcaagagcc
    10981 ggtcgccgct ccgacaggcc tacctgcgca gtcagatcca tccgatgctg ataaggcaca
    11041 aatagaagga gaagcaaact cgcgagcaga gtcggaaacc gccgcggcca gagcgcaatt
    11101 tgaaaatgat ttcggtgcac ctcctgaagc gttcgcaaaa cacggaccaa aagaacttcc
    11161 taaatatgag gagccgaaag gcatacctga gagcgtcaga caatatgtca cggatgccga
    11221 gatagtccct atttactgcg ctacggtcaa gtggaagacg ggcgagtttt tcacatcaat
    11281 gaacgcccta aagaacaaca tgcttcctgc aatgcaaaaa gtaaaaagcg acctaggcat
    11341 ttctatggat gcgccggaca ttgatggaat aatttccgaa ggacagagcc gagtcgatgc
    11401 tatctgcagt gctaaaacaa tagatgacgc ggacaagctt gcacgagatt tccagacatg
    11461 ggggctagat ccgaacaggg ttccgataga atcgtttatg caggagttcg ctaaaaaaat
    11521 gcaggagaag ggcgatgagc tacgaaagca gatagaggcg gctatccagc ctactatcaa
    11581 cgaagaaacc ccaaagatcc aagctgaaat cgaagccgag gccaagtcta tggcgggctc
    11641 tttcaaattt acgaaacagc caagtgccgc cgacatcgcg aatatgcgcg cacagataga
    11701 agaaaggctc aaacccatta tcgaacaaaa aaaagagcag gtgaagcaaa aagttatggc
    11761 caaaatcgag gagataaaga acggggaggc cggcaagttc caggaatacg gaaaactttt
    11821 tgaagggacg gaacaaaaga tagagacaga aagaaaggcc ggtctcagcg cttatgaaaa
    11881 atataaagag gaagcattca gactcaggaa agaggttgtc ttcaatattt tggacaaaaa
    11941 tctcgccgag gggatgaaac agcttgatgc cgccgaaaaa gatatcgagg aagccggaaa
    12001 gagcgaccct tctataagaa gcgtggcaga aataaaagcg gacattgagg cggacaagaa
    12061 ggtgctctcc tcaaaattag atgccgccct ggaagccgga gatgacgggg cttttgaatc
    12121 agctctcacg gactttagaa caaaatggga acaagtaaga aaagagggtg aatctctcgc
    12181 aaaacaatcc gcaagcaaag cctgcaatat ggccctcccc cagtttgaaa atgccaaatc
    12241 gcagatggat ggaaatatga gcaagattac aagcctcgag gccaagtgtg ccggaagcac
    12301 ctccgaggaa tgtttaacca tcaatgagtt ttcgggtaga ttcgccacgc tcaaatcaaa
    12361 gataaacgac attaaatcgg caatg
//
LOCUS       DAOTEX010000020        12218 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3118
            NODE_3649_length_12218_cov_4.035024, whole genome shotgun sequence.
ACCESSION   DAOTEX010000020 DAOTEX010000000
VERSION     DAOTEX010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868142
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 12218)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12218)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 883
            CDSs (total)                      :: 833
            Genes (coding)                    :: 829
            CDSs (with protein)               :: 829
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12218
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3649_length_12218_cov_4.035024"
                     /isolate="AMR_MDS_3118"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1037
                     /locus_tag="P5056_01895"
     CDS             <1..1037
                     /locus_tag="P5056_01895"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HRY62499.1"
                     /translation="GIFIFTSASLGILTDSVGQFQKTIIKQLSIFFIGWIALAFCAGI
                     NYKKWDKPAKYIYLASILLTLSVFIPGIGMDAKGARSWIDLGFTTFQPTEFMKLGFVL
                     LYASYLAGIKDKIKTFKYGVLPSIIILGVPVGILLLQNDLGSTLSPIAAGIAMFLVAG
                     GQKRYVAGGVIAAIIIVAITAFVSPHAMARIKTYIDPSQNLQGSGYHLNQSLIAVGSG
                     QIFGRGLGQSIQKFTFLPEATGDSIFPVAAEEFGFVGSTFIVLMFLAFGLRGYKIAGA
                     VPDVFGRLLVIGFVTLIFSQSFINIGTMLGVVPMTGLPLIFLSLGGTALLFSLFEMGI
                     VLNISRYQSR"
     gene            1275..2411
                     /locus_tag="P5056_01900"
     CDS             1275..2411
                     /locus_tag="P5056_01900"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HRY62500.1"
                     /translation="MKILFSGGGTGGHFYPIIAIAQGINQIVEREKLVGVNIYFMSDS
                     PYDERLLFENGITFKKASAGKLRKYFSLRNYFVDPFKTLWGIITATWKLFWIYPDVVF
                     GKGGYASFPALWAARILRIPVFIHESDSIPGRVNLIAGKWGITKRVACSYPEAMTYFP
                     EGKTALTGNPIRKELLEPTTKGSHEYFGLEKGVPIIFIVGGSQGAQNINDVIVDSLPL
                     LVEKYQIVHHTGKDNFRDVAGRAAFLLEKSEHKDRYKPYAFLDETALKMIAGAASLVI
                     SRAGSTIFEIAAWGLPSIIIPIPEEISRDQRKNAFSYARVSDAIVLEERNLSSSILTS
                     EIDRVLNDPQLQETMKVGAAKFAKKDAAIKIAEELIKLGLEHEK"
     gene            2412..3725
                     /gene="gltX"
                     /locus_tag="P5056_01905"
     CDS             2412..3725
                     /gene="gltX"
                     /locus_tag="P5056_01905"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HRY62501.1"
                     /translation="METPKTKAITRFPPSPTGNLHVGGLRTALFNYLYAKRMGGEMVF
                     RIEDTDKERSKKEYEQNILDSLNWVGIKVGEPWRQSERTEVYKGHLKKMIDSGLAYIS
                     KETPKEEGERAEVIRFKNPNKKIAFEDLILGTIEVDTTDLGDFVIAKDLETPLYHLAV
                     VVDDFDMGVTHIIRGQEHIANTPRQILIQEAIGAPRPFYGHIPLILAPDKTKLSKRHG
                     AVSVTQYKDEGYLPEVLVNFLALIGWNPGNDREFFTLDELCKEFSLEKVQKSSGVFDV
                     KKLNWMNKEYIKKLPKERLTELVLERVPKDKKDFPSFSQVFEKILPIIVDRIERFDEV
                     TKMFEAGEFDYFFADPVYEKSLLKNTSHLPKLLDILGGITDDNWNAAGIKSAVWDFAT
                     EVGRGEVLWPMRVSLSGREKSPDPFTLAEVLGKEETVRRIKMSLE"
     gene            3905..4450
                     /locus_tag="P5056_01910"
     CDS             3905..4450
                     /locus_tag="P5056_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62502.1"
                     /translation="MSNPVKYRPWGKNSLGLGLSFGDVFFKEERPLPDNFDPKKLELV
                     SLCSLGEKESLKERIGSLKEFYIFPSISDLEGEIPAHEWQNLVYLKPEITEVIRILTD
                     GGRIRTEVVLLGEVHKIRCRKSERSKKLVNIGLAAIQFREGGIEPPWPFQWGYRLAHI
                     SNPIVASGDAKNAFVLVYPVE"
     gene            4480..4701
                     /locus_tag="P5056_01915"
     CDS             4480..4701
                     /locus_tag="P5056_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62503.1"
                     /translation="MQNEELWEILCQMFASLYLMALAICGLKESWFLVWTLLFLLFAA
                     IVVRSSSSEVDDEAVVVVDDSDDEEEEKR"
     gene            4698..5045
                     /locus_tag="P5056_01920"
     CDS             4698..5045
                     /locus_tag="P5056_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62504.1"
                     /translation="MSTLLMVGKSMAAFDATMWVPFGLCMLLLIYLLPRTGIIASLFL
                     FLIRPAWFVIFLIMLFEPPFTVTFMVDMRVNGFLTAVGQYLFIKAILWTPFAFALYFD
                     YKNRSRLFRWFKK"
     gene            5182..6531
                     /locus_tag="P5056_01925"
     CDS             5182..6531
                     /locus_tag="P5056_01925"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HRY62505.1"
                     /translation="MFLLNKKRDVWFFSILLLCAGFSFMAVRALAENASPSESRSEID
                     TLKNEIETRNNEIKKLEEEIALLDKSIEKTQGQAKSLKNELSVINNTISQLNKQVKVT
                     ETKIQSTELNIQKLSIEIKDKEKNITEDKTSVGEMIQTMQQIDLTSPIEVVLSGDNFS
                     DAWVEVDYLERVQKSVNERINDLEIKKKSLEENKTELAAEKASLNSLRSRLLDQKNIA
                     NQGKKNKNSLLAETQNQEANYKKLLASTKAKKDNLERELFNYEAQLKVAIDPNSYPKA
                     GTSVLAWPVNPPYITQKFGKTSDSGRLYASGTHNGVDFRAKTGTPLVATAPGTVVGIG
                     DTDIACKGVSYGKWVLIRHTNGLATLYGHLSLIKAYAGEELQTGDIIGYSGNTGYSEG
                     PHLHFGLFVANAVRITGPTEYKSKVCGTYLRMPIAPTNAYLDPLAYLPITGSNIKTD"
     gene            6636..7778
                     /gene="alr"
                     /locus_tag="P5056_01930"
     CDS             6636..7778
                     /gene="alr"
                     /locus_tag="P5056_01930"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020260267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HRY62506.1"
                     /translation="MKNLGKTWVEVSKKNILHNIGEIRGLLGGAKFMAVVKSNAYGHG
                     LLEISKICAESKKVDWLGVDSVEEALAIRKAKIKLPILVLGYIPFEELKKAIENNISF
                     VAYNDELLSHLAKMKTAKKIKIHLKIETGISRQGLAGEELISFAKKALKNKNIFIEGA
                     YTHFANVDDTTDFTYAKKQLDAYEKNIAELEKIVHIPVKHTASSAATINYPETHFDMV
                     RTGISLYGLWSSKETKLSSKERKIKIDLRPALSWKTLLVQVKKVESGTPIGYGLTEKV
                     RRDSTIGILPIGYWDGYDRGLSNIGEVLVRGERAKVLGRICMNMTIVDLTDVKGARLF
                     DEVVLLGKQGNEEITADEIAGKLGTINYEVVTRINPMISRIIDGGK"
     gene            7865..8272
                     /locus_tag="P5056_01935"
     CDS             7865..8272
                     /locus_tag="P5056_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62507.1"
                     /translation="MNRLSLGKVELSRVVSRVKLQYSISDNFENEQDESDALKISDLI
                     EEDKKASEVCVMLSFFAGFVVFLSVVSLGTLVTAQFYLLLGVAMGILTGFAFYYLYSF
                     FSGKSKLDIFLGADNRKKKIADKVKILRAELGL"
     gene            8452..8958
                     /locus_tag="P5056_01940"
     CDS             8452..8958
                     /locus_tag="P5056_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62508.1"
                     /translation="MRKVINLLFLAVLCFFAYNTWQTNELNSHFTLLYTSEFSTQKDI
                     RITDGDEERTIAFAGKTYEASVYMIKPRFRGVSKSQCLRFVMSKNAYLVGFYTQYVIS
                     QDIADMVTRNGEIREFLMFKGSDHEPICIKRCPPREDEKFSRTFEVEIWPREPWTYGT
                     AILYIKEK"
     gene            8972..9442
                     /locus_tag="P5056_01945"
     CDS             8972..9442
                     /locus_tag="P5056_01945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62509.1"
                     /translation="MNLSDPHNGARLSLWITIIIFICFAVGGMGACMMDSSDKYLLIK
                     KEDYDKFAESYKTLERAYDAIIKEANVLREERDALLKRVEELEAELAKEKSKTTGEVL
                     DHKAHICFRCYQNLDTSLTKDEMAVEENLLLERARIMKFTRWRLNILPKIFEVK"
     gene            9643..10194
                     /locus_tag="P5056_01950"
     CDS             9643..10194
                     /locus_tag="P5056_01950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62510.1"
                     /translation="MRFLAWALILFSCALSAGAIWFLALIWIQTTPFFIGLFATPIHE
                     MCHLDAIGAVEWYTGKNAIILKVSLFNADFNSLTDFLRSIMEMDESARYMGYVKWFDE
                     DEFSWEERFFVSLMGGVGSTIFILCLWFASWKCFMRGVGTNIFDYTIIFVFCHAPFIL
                     ATMESLDYAFLEASRMGAPIIFL"
     gene            10302..10511
                     /locus_tag="P5056_01955"
     CDS             10302..10511
                     /locus_tag="P5056_01955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62511.1"
                     /translation="MIKQTQNGGFIKLILLFVVGIIVLSYFGFNLREILNSDAVKNNF
                     LAVWDFLSGLWTNYCVPLFSKFFTS"
     gene            11131..11346
                     /locus_tag="P5056_01960"
     CDS             11131..11346
                     /locus_tag="P5056_01960"
                     /inference="COORDINATES: protein motif:HMM:NF015947.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HRY62512.1"
                     /translation="MWTKKVFKVGDSIAVTLPVEICRTFNIKRGDYLVVTIGKDHEIQ
                     LIKFDPNKRPDLLDFEEREVPIINFTN"
     gene            11359..12108
                     /locus_tag="P5056_01965"
     CDS             11359..12108
                     /locus_tag="P5056_01965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HRY62513.1"
                     /translation="MNDTTKNISLDLIDDPKLAMRSDVDSDDIEELCSSMREIGLVEP
                     IVVRPNGERYEVIAGHRRTRAARILGWPVILAVIKQADDNDTLAMRLAENLSRHNVDP
                     VDEACFIGEIMKTYSFTANDVAEKLKRRLEWVEERLEVFQMPQYMQQHLKLKKYPLGA
                     ALWISRIENEATKNYYANWAAINGVSVAGAKQWHDNLKATNFNVDLTSVEIKDEGGQV
                     QQVVKMIKCAACHEDCELSKADSVWVHPTCH"
BASE COUNT         3579 a         2290 c         2942 g         3407 t
ORIGIN      
        1 tgggaatttt tatttttaca tccgcatctt tgggtattct cacagatagc gtcggtcaat
       61 tccaaaaaac tataatcaaa cagctttcca tcttttttat cggctggatc gctctcgctt
      121 tttgcgccgg tataaattac aaaaagtggg ataagccggc gaaatatatt tatctcgctt
      181 ctatactttt aacattgtcg gtttttattc ccggcattgg aatggacgca aaaggcgcga
      241 ggagctggat cgatcttggc tttacaactt tccagccgac agaatttatg aagctcggtt
      301 ttgttctcct ttatgcatca tatttggcgg gcatcaaaga taaaataaaa acatttaagt
      361 atggggttct gccgtcaatt attattttgg gagtgcctgt cggcatcttg cttttgcaga
      421 acgacctcgg gtctacactt agccctatcg cggccggcat cgcaatgttc cttgtcgcgg
      481 gtggacagaa gagatatgtc gcgggcggtg ttattgccgc catcataatt gtcgcaatca
      541 cggcatttgt atctccacac gctatggcaa gaataaaaac atacatcgat ccttcccaaa
      601 atcttcaagg ttccgggtat cacctcaatc agtcgctcat tgctgtcggc tccggacaga
      661 tattcggcag agggcttggc cagagtattc aaaaattcac attcctcccg gaggcgacgg
      721 gggactctat tttccctgta gcggccgaag aattcggttt cgtcggaagc accttcatcg
      781 tgctgatgtt tttggccttc ggtcttcgcg gatacaagat tgcgggggcg gtgccggacg
      841 tcttcgggag attgcttgtt atcggttttg ttacccttat cttttcacaa tctttcataa
      901 atataggcac gatgctcggg gtggtgccga tgaccggact gccgctcatc ttcttgagcc
      961 ttggaggaac ggcacttctt ttttcgttat ttgaaatggg tatagttttg aatatatcca
     1021 gatatcaaag tagataaaac acccacactc gctgtcattc ccgccccccg atcgcggtcg
     1081 agggtaaact ccggtgggaa tttagttttt aagcggagac acacgctgga ttcccgcctg
     1141 cgcgggaatg acaaacaggg agatacttcg gaagtgcctg gattaaggaa gattaatccc
     1201 gtataatgga tatgtgatgt ggtttatgta aaaactctta attctttatt cttaattcat
     1261 acttcttttt aaaaatgaaa atacttttta gcggaggggg gactggtggt catttttacc
     1321 ccataatcgc gattgctcaa ggcataaatc agatagtaga gagagaaaag ctcgtcggag
     1381 tgaacattta ttttatgtct gattcgcctt atgacgagag acttcttttt gagaacggca
     1441 taacattcaa gaaggcttcc gccggcaagc tcaggaaata tttttcttta agaaattatt
     1501 ttgtagatcc gttcaaaact ctttggggca taattacggc tacatggaaa ttgttttgga
     1561 tatatccgga cgtcgtgttt ggtaagggcg gatacgctag cttccctgca ttatgggcgg
     1621 cgaggatatt gcgcataccg gtattcatac atgaatccga ttctattccg gggagggtca
     1681 atctcatcgc cggaaagtgg ggaatcacaa aaagagtcgc atgctcttat cctgaagcaa
     1741 tgacatattt cccggaggga aagaccgcgc tcacgggcaa tccgattaga aaggagcttc
     1801 tcgagccgac gacaaaaggt tctcacgaat attttggttt agaaaaagga gttccgatta
     1861 tttttatagt cggaggttca cagggagccc aaaacataaa cgacgttatc gttgattcat
     1921 tacccctact tgttgagaaa tatcagatcg tccatcacac aggaaaagat aattttaggg
     1981 acgtcgccgg ccgcgcagcc tttcttttgg aaaaaagtga gcacaaagac agatacaagc
     2041 cttacgcgtt tttggatgag acagctctga agatgattgc gggtgccgcc tctctcgtga
     2101 tatcgcgcgc gggatcaact atctttgaaa ttgcggcatg gggacttcca agcatcataa
     2161 ttcctatccc cgaagagata agtagggacc agaggaagaa cgcattttct tacgccagag
     2221 tcagcgacgc catcgtcctt gaggaaagaa acctcagctc ctcaattctc acctcggaaa
     2281 tagatagagt gcttaatgat cctcagctgc aggaaacaat gaaagtgggt gccgcaaaat
     2341 ttgcaaaaaa agacgcggcg attaaaatag ccgaagagct aattaagctc ggacttgaac
     2401 acgagaaata aatggaaact ccaaaaacaa aggcgataac gcgctttccg ccgtcaccaa
     2461 caggtaattt gcatgtgggc ggtctccgca cagcgctttt taattatctt tacgccaaaa
     2521 gaatgggcgg cgagatggtt tttagaattg aagacacaga caaggagaga tcaaaaaaag
     2581 aatacgaaca aaatattttg gactcactaa attgggtcgg aataaaagtc ggcgagccat
     2641 ggaggcagtc ggaaagaaca gaggtctaca agggacacct gaagaagatg atcgattccg
     2701 gactcgcata tatttcaaag gaaactccga aagaagaggg ggaaagagcc gaagttatac
     2761 gtttcaaaaa tccgaataaa aagattgctt ttgaagattt gatattgggc acgatagagg
     2821 tggacacgac cgatcttggc gacttcgtaa tagcaaaaga cctagaaaca ccgctctatc
     2881 atttggctgt tgtggtggat gattttgaca tgggcgtaac gcacataatt cgcggtcagg
     2941 agcacattgc gaacacacct agacaaatcc tcattcagga ggcaatcggc gctcctaggc
     3001 cattttacgg ccatataccg ctaattttag ccccagataa gaccaaattg tccaaaaggc
     3061 acggtgctgt gtctgtaacg cagtataagg acgaaggata cttgcccgag gtgcttgtga
     3121 acttccttgc ccttatcgga tggaacccgg ggaacgacag agaatttttc actttagatg
     3181 agctttgtaa ggagttctct ttggagaaag ttcagaaatc gagcggtgtc tttgacgtga
     3241 aaaagttgaa ctggatgaac aaggaatata taaagaaact tccgaaagag agactcacag
     3301 aacttgtcct ggaaagagtt ccgaaagaca aaaaagattt tccttcgttc tctcaagttt
     3361 ttgaaaaaat acttccgata atcgtagaca ggattgagag atttgatgag gttacaaaaa
     3421 tgttcgaggc gggtgagttt gattatttct tcgccgatcc ggtctatgaa aaatcgcttc
     3481 taaagaacac gtcgcatcta ccaaaacttc ttgatatttt gggtgggatt acggatgaca
     3541 actggaacgc ggcagggata aaatctgctg tttgggactt cgcaacagag gtgggcaggg
     3601 gagaggtttt gtggccaatg agagtatcgc tctccggccg cgagaagtct ccggacccat
     3661 tcacactcgc ggaggtgctg ggtaaagagg agaccgttcg aagaataaaa atgtccctag
     3721 aataatattc tcttgtcatt gctagtgaag cgcctgcctg cgcaggcagg caatccagga
     3781 aaaatgtcat tcccgtgaaa acgggaatcc atttttttct ttgacaaatc gtcaaaaagg
     3841 tgtagtttta ttgaagatat ttagaacttt aaaaacaaaa agactacaga aagggtgatg
     3901 ctgaatgtct aatcctgtta agtacaggcc ttggggtaaa aattcactgg gactcggttt
     3961 aagtttcgga gacgtctttt ttaaagaaga gaggcctctg cccgataact ttgacccgaa
     4021 gaaactggaa ctcgtatccc tttgtagtct tggagaaaaa gagagcttaa aggaaagaat
     4081 cgggtctctt aaggagttct acatttttcc gtcaatttcg gatcttgagg gagaaatccc
     4141 ggctcatgag tggcagaatt tagtctattt gaagccggag atcaccgaag ttattagaat
     4201 cctcacggat ggtggccgta ttcgtaccga agtagttttg ttgggagaag tgcataagat
     4261 aagatgcagg aaatcagaaa gatccaagaa actagttaat atcgggctgg ctgctatcca
     4321 attccgagag ggaggcatcg agcctccctg gccatttcag tgggggtacc gcctcgcgca
     4381 tatctccaac ccaatcgttg ctagcgggga cgccaagaac gcttttgttc tggtttatcc
     4441 tgtcgaataa taaacgccaa cagaaaggga tgatgtctga tgcagaatga agaactttgg
     4501 gagattttgt gccagatgtt cgctagccta tatctcatgg cgcttgcgat ttgcggctta
     4561 aaggagagtt ggtttctcgt ttggactctc ttgtttttgc ttttcgcggc tattgtagtc
     4621 cgcagttctt cctcagaagt ggacgacgag gctgttgttg tcgtcgatga ttcggacgat
     4681 gaagaggagg aaaagagatg agcacattgc ttatggtggg aaagtccatg gccgcttttg
     4741 acgccaccat gtgggttccg ttcggtcttt gcatgttgct ccttatctat ctgttgccac
     4801 gtacggggat tatcgcgtct ctctttctct tcctcattcg tccggcatgg tttgtgatat
     4861 ttctcatcat gctttttgag cctcctttta ccgttacctt tatggttgat atgagagtga
     4921 atggattttt gaccgcggtg ggtcagtacc tttttatcaa agccattctt tggaccccgt
     4981 tcgctttcgc gttgtatttc gattacaaaa atcgaagcag acttttccgc tggtttaaga
     5041 agtaaacaaa agcccctcta tggaggggct ttttctcctc caaaattttt gtccgttatt
     5101 ggtttgtttg aagataaaat tacaaaaggt gaaaaattta ggcggataaa tcttcgcaaa
     5161 tgtgataaaa ttaaatgaca gatgtttttg ctgaacaaaa agcgagatgt ttggtttttt
     5221 tctatactgc ttttgtgcgc gggtttttct tttatggccg tgagagcgct tgcggaaaat
     5281 gcatcgccgt ctgaaagtcg ttccgaaata gatacgttaa agaacgagat tgagacaaga
     5341 aataatgaaa taaaaaagtt ggaggaagag attgcgctat tagataagtc tatagagaaa
     5401 acacaggggc aggctaaatc gttaaaaaat gaattgagcg taataaataa tacaatatct
     5461 caattaaata agcaggtgaa ggttacggag acaaagatac agtcgactga attaaatatt
     5521 caaaagcttt ctatcgaaat aaaagataaa gaaaaaaaca tcacagaaga caaaacttcc
     5581 gtcggggaaa tgatacagac aatgcaacag atagacctaa cttcgccgat agaggtggtt
     5641 ttgtctggcg ataattttag tgatgcgtgg gtggaggtgg attacctaga gcgcgttcaa
     5701 aaaagtgtga atgaaaggat aaacgatctc gaaataaaga agaaaagtct tgaagagaac
     5761 aagaccgagc tggccgcaga aaaagcgtca ttaaatagtt tacgttcgcg ccttttggat
     5821 caaaaaaata tcgccaatca gggtaagaaa aataaaaaca gcttgcttgc cgaaacccaa
     5881 aatcaggagg cgaactataa aaagttgctg gctagcacaa aggcaaagaa agataattta
     5941 gaaagagagc tttttaatta tgaagcacag cttaaggtgg ccattgatcc aaatagctat
     6001 ccgaaggcag ggacaagcgt tttggcttgg ccggtaaatc ctccatatat cactcagaaa
     6061 tttggcaaga cgagtgattc aggtagattg tatgcttcag ggacgcataa cggagtcgac
     6121 tttagggcaa agacagggac gcctcttgta gctacggcgc ccgggaccgt cgtcggaata
     6181 ggagatacag atatcgcgtg caagggcgtt tcttatggca aatgggttct tataagacac
     6241 acgaacggct tggctactct ctacgggcac cttagcttga tcaaggctta cgccggggag
     6301 gagctacaaa caggagatat tattggttat agtggtaata cgggctactc ggaggggcca
     6361 catttgcact ttggactctt tgtggcgaac gcggtaagaa taactggtcc gaccgagtat
     6421 aagagtaagg tctgtgggac gtatctaagg atgccgattg cgccgacaaa tgcttatctt
     6481 gatccgctcg cgtacttgcc gattacgggt agtaatataa aaaccgatta aggttgtttg
     6541 tcattcccgc gaaggcggga atctatattt actgctctat actggattcc cgccttcgcg
     6601 ggaatgacag aggggggtaa gattgcaata aaatcatgaa aaatttggga aaaacttggg
     6661 tagaggtttc taaaaaaaat attttacaca atattggcga aatacgcgga cttttgggtg
     6721 gtgcgaaatt catggcggtc gttaaatcaa atgcctatgg acatggtctt ttggaaatat
     6781 caaaaatttg cgcggaatca aaaaaagttg attggcttgg cgtggattcg gtagaagaag
     6841 cgctcgcgat aagaaaggca aaaataaaat tgccgatact cgtgctcggg tatattccct
     6901 ttgaggagct aaaaaaagcc atagaaaata atatttcttt tgttgcgtat aatgacgagc
     6961 ttttaagtca tctcgcgaag atgaagaccg caaagaaaat taaaatacac ttaaagattg
     7021 aaaccggcat ttctcgccaa gggcttgcgg gcgaagaact tatttctttt gctaaaaaag
     7081 cccttaagaa taaaaatatt tttatagaag gtgcatacac acattttgcg aatgttgatg
     7141 acactacaga ttttacttat gcgaaaaaac agcttgatgc ttatgagaaa aatatcgccg
     7201 agctcgaaaa gatcgttcac atcccagtca agcataccgc ttcgtccgcg gccactataa
     7261 attatccgga gacgcatttt gatatggtca ggacaggcat ttctctttat gggctctggt
     7321 cttcaaagga gacaaaactc tcatcgaagg agcgaaaaat taagatagat ttgcgaccgg
     7381 cgctttcatg gaagaccctt ttggtgcagg tgaagaaggt tgagtcggga acgccaatcg
     7441 gatatggtct cacagaaaaa gtcagacgag attctactat aggcattttg ccgatcggtt
     7501 attgggatgg atatgataga gggctctcaa atatcggcga agtgcttgtg agaggggagc
     7561 gtgccaaagt cctcgggagg atatgcatga atatgactat agttgaccta acggatgtta
     7621 agggggcgag gctttttgat gaagttgttt tgctcggaaa gcaggggaat gaagaaataa
     7681 ctgcagatga aattgccggt aaacttggaa caataaatta tgaagttgtg acacgaataa
     7741 atccgatgat tagtaggata atagacggtg gaaagtagat gatggtcata aaatatcttt
     7801 acattacaac ttaaagaaag tagtatttag acagaaaaag aacattaaaa aagggggatg
     7861 aggtatgaat cgactttcct tgggcaaagt tgaactgagc cgggttgtct ctcgcgtaaa
     7921 gttgcagtac tcaattagtg ataattttga aaatgaacag gacgagtcag atgcgcttaa
     7981 aatttctgat ttgatcgaag aggacaagaa ggcttctgaa gtgtgtgtca tgttgtcatt
     8041 ttttgccgga tttgttgttt ttctgtccgt tgtctctctc ggcacgctag tcacggcaca
     8101 attttatctt ttgcttggcg tggcaatggg aatcttaacg ggatttgcat tttattacct
     8161 ttactcgttc ttttctggta aaagcaagct tgatatcttc cttggtgcag acaatcgcaa
     8221 gaaaaagatc gccgataagg ttaaaatttt aagagccgag ctcggtctct agcgaccata
     8281 gcctccccaa aatcggggag gttttttatt ttatattatc aataagaaaa ggtacccagt
     8341 agtagaattt ggcaacgcac atagtgcggc ttgacgcctt tgccaaattt cactacgggg
     8401 tgtagtattt ggcttgaaaa aaagaagttc cttgaaaggg gagttgctac gatgagaaag
     8461 gtcattaacc ttctgttttt agccgtcttg tgcttttttg cttacaatac ttggcaaaca
     8521 aatgagttga atagccactt tactcttctc tacacgtcgg aattttcaac gcaaaaggac
     8581 attcgtatca ctgatggcga cgaagagaga accattgcat tcgcgggcaa gacttacgaa
     8641 gcgagcgtgt acatgatcaa gccgagattc cgtggcgtca gcaagagtca gtgccttaga
     8701 ttcgttatga gcaaaaatgc ttatcttgtt ggattttata ctcagtatgt tatcagccaa
     8761 gatatcgccg atatggtcac aaggaacggc gagattaggg agttccttat gtttaagggt
     8821 agcgatcacg agcctatctg tatcaagagg tgcccacctc gggaggatga aaagttctct
     8881 cggactttcg aggttgaaat ttggcctcgc gaaccttgga catacggcac tgctattctg
     8941 tacatcaaag agaaataagg aggcgagcga aatgaatttg tctgatcctc acaacggtgc
     9001 caggttgagc ttatggatta caattataat tttcatatgt tttgccgtgg gcggtatggg
     9061 cgcttgcatg atggatagct cggataagta tttactcatc aaaaaagaag attacgacaa
     9121 atttgccgag agttacaaaa cactggagag ggcctatgac gccatcatca aggaggccaa
     9181 cgtgcttcgc gaggaacgcg atgcgctcct gaagcgtgtc gaagagctcg aggcggagct
     9241 tgctaaggaa aagagcaaga cgactggcga ggtcctcgat cacaaggccc acatttgttt
     9301 ccgctgttat cagaatctcg acacgagcct gaccaaggat gagatggcgg tcgaagagaa
     9361 tcttctcttg gagcgagcgc gaatcatgaa atttacgcgc tggcgactta atattctccc
     9421 caaaattttt gaagtgaagt aaaatttggc cacgtagaga tacgtggctt ttttacccgc
     9481 ttcacccgcc aaaatttatg aaaataaatt taggcgggca agaaattttc tggattgaac
     9541 aatttagaag ggtgtcttta caaaaatttt atatatgata ttttcaatta aatagttcgt
     9601 tggaaaataa cacataaata aagaggagaa ggcggtgtgc ttatgcgctt tttggcctgg
     9661 gctttgatct tgttcagctg cgcattgagt gccggggcca tttggttttt ggcgcttatt
     9721 tggatacaga caaccccgtt ttttatcgga ctgtttgcta ctccaatcca cgaaatgtgt
     9781 cacctagatg ccatcggcgc ggtcgagtgg tataccggca agaatgccat aattctcaag
     9841 gtgtctttgt ttaacgcaga ctttaacagc cttaccgatt ttctccgctc tattatggag
     9901 atggacgagt cggcgagata catggggtat gttaaatggt ttgatgaaga cgaattctct
     9961 tgggaagaaa gattttttgt aagccttatg ggcggtgtgg gcagtaccat atttattctg
    10021 tgtttgtggt ttgcgtcatg gaagtgcttt atgaggggag tgggaacaaa tatttttgac
    10081 tacacaatca tatttgtctt ctgccatgct ccttttatac tagcgacaat ggagtctctc
    10141 gactacgctt ttctagaagc tagccggatg ggagctccga taattttctt gtaggatttt
    10201 gggtgtcttt taacaaggac gccctttctt tttaattgat tggcaaaata ttgtgcgacg
    10261 cgatgtaggg ttctcttttg ggaatgtgct ataatattta catgatcaaa caaacacaaa
    10321 acggtggttt tataaaatta atactacttt ttgtcgtcgg aattattgta ttaagttatt
    10381 ttggttttaa tttgagagaa atcttaaatt ccgatgcagt aaaaaataat tttttagcag
    10441 tttgggattt tctaagcgga ttatggacta attattgtgt accgcttttt tctaaattct
    10501 ttacttccta aaattgcccc agggaagaga agtgggtggt accccgtagt gaaatttggc
    10561 aatggccaaa ggccgcgcca cgaagcgcgt tgccaaattc tactacgggg tggtaggtag
    10621 atacctggag ttagcttaaa aaccctaaaa caggtaaatt aggtcaaaag gacaaatttc
    10681 gtgatacata tgtatcacga aatttttata gagacccgcc aaagggtgag gggcggacga
    10741 ttaactactc atcaaaaata tttgcgcacg tcctagatga ttttagaggg tcgttttagt
    10801 ctgaaaatta taaaactatt aaggggaggg gctatttcat aaatagccat atttttgtat
    10861 atattgtgtc gcaaaatata tcatacattt tgcgatgacg tcgcaagcgg cgttgcaaaa
    10921 tgtgcattgt gcgacagttt aaagattctt ccgtatgtcg tcaattacta ttctatctga
    10981 aataaaaaat tcatcaagca taatttttta tttcactgtg gataactttt gaaaataggg
    11041 gaagtagccc ctagctagat tatacgaatt attataatat attagttagt gtgtgaagct
    11101 taagctaatt aaaagttaac ttatgcttct atgtggacta aaaaagtttt caaagtaggc
    11161 gatagtatcg ctgtaacttt gcctgtggaa atttgtcgaa cgtttaatat taaacgtggc
    11221 gactatttgg tcgtgactat tggaaaagat cacgagatcc aacttataaa atttgatccg
    11281 aacaagcgtc cggatctttt ggacttcgag gaaagagaag tgcctataat aaattttact
    11341 aactaatttt tttatacaat gaacgataca acaaaaaata tttctctcga tttaatagac
    11401 gatccaaaac tagcgatgag atcagacgtc gactctgacg acatcgaaga actatgttct
    11461 tcgatgagag agatcggact cgtcgagccg atcgtcgtca gacctaacgg cgagagatat
    11521 gaagtgatcg ccggacacag aagaacacgc gccgcgcgta ttcttggctg gccggtgata
    11581 cttgctgtca tcaagcaagc agatgacaat gatactctcg ccatgcgact cgctgaaaat
    11641 ttatcgcgac acaatgttga tcccgtcgat gaagcatgct tcatcggaga gattatgaag
    11701 acatacagct tcactgcaaa tgatgtcgca gaaaaattaa agcgtcgttt agaatgggtt
    11761 gaagaacgac tagaggtgtt tcaaatgccg caatatatgc aacagcattt aaaactaaaa
    11821 aaatatcctc taggcgcagc gctttggatc tcaagaattg agaatgaagc tactaaaaat
    11881 tattatgcca actgggcggc gatcaatgga gtatcagtcg ccggcgcaaa acagtggcat
    11941 gataatttaa aagccaccaa ctttaatgtt gatcttacta gtgtcgaaat taaagatgag
    12001 ggcggtcaag ttcagcaggt agttaaaatg atcaagtgtg cagcctgtca tgaagactgc
    12061 gaactctcta aggcagattc cgtttgggtt cacccaacct gtcactagat actatatagg
    12121 ggtatagttt tatccttata gagattattt taaaattaag ataagccgcc gccttttttg
    12181 ggcggcgtcc tattgtgtgg ggggaattta tttattta
//
LOCUS       DAOTEX010000021        11801 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3118
            NODE_3808_length_11801_cov_4.061042, whole genome shotgun sequence.
ACCESSION   DAOTEX010000021 DAOTEX010000000
VERSION     DAOTEX010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868142
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 11801)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11801)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 883
            CDSs (total)                      :: 833
            Genes (coding)                    :: 829
            CDSs (with protein)               :: 829
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11801
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3808_length_11801_cov_4.061042"
                     /isolate="AMR_MDS_3118"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="P5056_01970"
     CDS             <1..351
                     /locus_tag="P5056_01970"
                     /inference="COORDINATES: protein motif:HMM:NF015113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HRY62514.1"
                     /translation="GTVVEVLSDEMGIVWPESIAPYKVHLISLAGKKGGEKVKEKAEE
                     IYAYLTKRGVEILYDDRDSSAGEKFSDSDLIGIPKRIIVSQRGIEEGSFEMKDRKTGE
                     VTKVTEKELSEMFK"
     gene            348..1400
                     /locus_tag="P5056_01975"
     CDS             348..1400
                     /locus_tag="P5056_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HRY62515.1"
                     /translation="MIKKFYKYFSSDIGIDLGTANTLVFLRDHGIVIDEPSVVAINQK
                     TNRVVAIGKEAKMMVGRTPHHITAVRPLVDGVISDFEITEEMIGYFLRKTQEISNKKS
                     LRPRVLIGVPSGITNVERRAVRDAAMNSGAREVHLIEEPMSAAIGVKLPVHEPMGSMV
                     IDIGGGTTDIAVISLNGIVQSKNLRVAGDKFNQDIISYIRDEFKILLGEKTAEDIKIA
                     IGSACEQDSPMEYIVKGRDLVTGLPREVVVTDHDVREAMSNSLSLIVDSVKEVVERTP
                     PEIVADVMNKGVVLAGGGSMIRGLAHVLQKEAKIPVHMAEDPLRAVVRGTGTVLENFD
                     LYEEVLIDNEDEIPPQ"
     gene            1432..2214
                     /gene="mreC"
                     /locus_tag="P5056_01980"
     CDS             1432..2214
                     /gene="mreC"
                     /locus_tag="P5056_01980"
                     /inference="COORDINATES: protein motif:HMM:NF016011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HRY62516.1"
                     /translation="MAAIILSVGCLYMWYSPITSALYSAGVFITAPFWSMQGYLSDSL
                     ASFMINFDSKKALIEKNDYLTKQLNSFKSTALENKLIRDENIELKKILGREEPSASGE
                     KPAASKTKKLLAYVLSGPVTPPYDSLIIDVGSDMGVAQGDLVLNDDNYVMGEVVEATS
                     GTSKVRLLSAYGQKTGVLLGDNKTMIEMVGQGSGNFFIAVPKEFLVKESDSAIFPGPD
                     FKMVANVESIEGKDGEMFKNVYLSAPFNIKEIRMVAVVKKII"
     gene            2497..4185
                     /locus_tag="P5056_01985"
     CDS             2497..4185
                     /locus_tag="P5056_01985"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRY62517.1"
                     /translation="MRINNRFNKDIEPDEIFLDSTNLSSLNTQQFEGWIEKPISKRVI
                     TLTFIFFAAVVCIFSWKVWSLQIKNGESLAKRSERNSLKKVSFPPERGIIYDRNGKMV
                     AWNDKDARSYYAEPGLYSILGYLGYPTKKDLETVENPKERLGREGVEKIFNEILRGQD
                     GVKMVEVDALGEVQSESVYNPGVKGGDIYLSLDAEMSSVWYKAIRDLAADKGFQGGAG
                     IMMDIKTGEVIAMTSFPEYDSNAIVRKDEAKINEYLKDKRMPFLNRVTLGLYTPGSVM
                     KPIFALGALNENIIDPKKEIYSAGFISIPNPYFPDKPTIFKDWKAHGWVDMQRAIAVS
                     SDVYFYEIGGGYEGQKGLGISNLEKYARMFGIGTTTGFDIGPEENGNIPSPAWKAENF
                     DGEKWSIGNTYHSSIGQYGFQVTPIQMVRAIAAIANDGHLVVPTILKASNSDWGRVSS
                     VIDIDKNKFDIVKEGMRRSVTEGTSMGISFPKPRIASKTGTAELGALKQYVNAWNVGF
                     FPYESPKYAFAVVMERGPVKNTVGGVYIMNQLFNWMIIHRPEYVSPTTSEIINP"
     gene            4296..4763
                     /gene="greA"
                     /locus_tag="P5056_01990"
     CDS             4296..4763
                     /gene="greA"
                     /locus_tag="P5056_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003361709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HRY62518.1"
                     /translation="MTTNIQYLSQEKINELKAELNDLKTKKRKEITESLEFAKSLGDL
                     SENAEYQEAREMQANVEQRISDLEAILKDAIIVSTNHKDIVGMGSTVIVKVEPGKETK
                     EFQVVGSEEANVLVGKISNLSPLGTALVGKKVGSTVTVNTPKGAVQYKILEVK"
     gene            5040..6503
                     /gene="lysS"
                     /locus_tag="P5056_01995"
     CDS             5040..6503
                     /gene="lysS"
                     /locus_tag="P5056_01995"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HRY62519.1"
                     /translation="MASIEEIREDRIKKLNILKEKGIDAYPARSHRDFYISEAIERFD
                     ELAKKDSIFMVGRVMALRGQGALIFFNFYDGTATFQGLLKKGDIDDSKLDLFNITIDI
                     GDFIEVFGNLFVTKRGEKTIQVKDWRILSKSLRPLPGEYYGFKDEEERLRKRYLDILV
                     NEDIKDRVLKRTKFWASIRNFLVNKGFAEVETPVLENTAGGAEARPFITHHNALDIDV
                     YLRISAGELWQKRLMVAGLPKVFEIGRIFRNEGMDAEHLQDYTQLEFYQAYSDYNEGM
                     EMIKEMYRYVAKETFGTSKFNIKGFEIDLDDDWKTYDYVETIKKMIGVDIWTAELSDM
                     ENKLRELKVEYDAKGFNKTRAIDNLWKFCRKQIGGPGFLINVPVFMEPLAKRKEGDDR
                     VVERFQVILAGSEVGKGFSELNDPIDQEERFMEQATLREGGDEEAQMHDHDYVEAMEY
                     GMPPTFGFGVSERLFAFFVDKPARECQIFPLMRPKEE"
     gene            complement(6722..7054)
                     /locus_tag="P5056_02000"
     CDS             complement(6722..7054)
                     /locus_tag="P5056_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62520.1"
                     /translation="MIIDGTTKEGRNYPIRPKHINTKRHFFSAFGRAEPESSAALIVR
                     LCQTRGGWLPFTQEEIDGFIGKDFYFHDLCGFDHRNPGNMVILGDDDKYRVTHEFIVR
                     CFQSSPAF"
     gene            complement(7102..7272)
                     /locus_tag="P5056_02005"
     CDS             complement(7102..7272)
                     /locus_tag="P5056_02005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62521.1"
                     /translation="MPTQTQMGRYLLNKIFYVAEEIALFTLMVIETCHGNYDRAGLAL
                     VSIIMVGYMCDR"
     gene            complement(7414..7578)
                     /locus_tag="P5056_02010"
     CDS             complement(7414..7578)
                     /locus_tag="P5056_02010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62522.1"
                     /translation="MKPYGFLHMLLALVLMIAALSNSLPPGVGRLLVFVWGFSSITIG
                     AHRRLSGMTD"
     gene            complement(7633..7926)
                     /locus_tag="P5056_02015"
     CDS             complement(7633..7926)
                     /locus_tag="P5056_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62523.1"
                     /translation="MTFYTIYATLKKELKSSQFLKFQSGAKRLIVANGGSNMNKTLCF
                     FFIINCLLAAGLIWVQSVLSTTTYYPGDGIMGLAGLFLGYFIARAEFKYMNTH"
     gene            8200..8634
                     /gene="mraZ"
                     /locus_tag="P5056_02020"
     CDS             8200..8634
                     /gene="mraZ"
                     /locus_tag="P5056_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HRY62524.1"
                     /translation="MLIGEHKHTLDPKKRVSLPAKFRKEMGKEVVLGIGLDKCLSVYT
                     VAEWEKYSARLSEMSMLQSDKRSFNRLILGSASLVEVDSLGRILISDVLKEYAGLKEK
                     VAIIGVRSNLEIWDEDAWNEYKGKVAKNADVLAEKLGELGAF"
     gene            8638..9540
                     /gene="rsmH"
                     /locus_tag="P5056_02025"
     CDS             8638..9540
                     /gene="rsmH"
                     /locus_tag="P5056_02025"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008412289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HRY62525.1"
                     /translation="MAHIPVLLKEVIEGLNLKPGMVVLDATLGGAGHSKEMCKLIGKE
                     GLLVGIDEDKAAIGRAEVALKDLCRFNLFLSNFRELSTVLQSAGVPQIDAALFDLGLS
                     SFQLEESKRGFTFLKDEPLMMTFEEAPSEDKLTAGEIVNEWAEESLADIIYGYGGERY
                     ARRIAKGIVEAREKKPIETTFELVEIIRNSVPVIYQKRRIHFATKTFQALRITVNDEI
                     GAVKKALEDVWGKVKVGGRVAVISFHEIEDRAVKTFFKGRKEFGDAKLITKKPIVPGE
                     DEVLENPRSRSSKLRIAEKINAII"
     gene            9602..9868
                     /locus_tag="P5056_02030"
     CDS             9602..9868
                     /locus_tag="P5056_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62526.1"
                     /translation="MMLLSPMYGYLVLSSVVNISQREKLSDEISAITAKVSVLEANYI
                     TMRSAINIDVAGKLGMKEEFNKINFASADKGDVKGGLSYVNNEI"
     gene            9849..11579
                     /locus_tag="P5056_02035"
     CDS             9849..11579
                     /locus_tag="P5056_02035"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HRY62527.1"
                     /translation="MSIMKYDHSPRISFLVYASVLFAAILIARLFEIQIVRGSDYSEM
                     AARQYSRPMPDIFSRGTIYFTEKNGNLVSAATLQTNFVLAVNPEAIKDAGATFKSLSP
                     FVDFSEEEFLKRASKEDDTYEVVGKIKGEDEFEKINELKLAGVFTFREKSRFYPAGRL
                     ASHVLGIVSRSKEEGERYIGRYGAEKQYEDLLHRDSESVYVNFFAEIFSNVKKTILGD
                     GNNLSGDVALSIEPTVEGVLEKELVNIQEAWKGKSSAGIIMEPKTGKIVAMAALPDFD
                     PNYFGKEKSVSVFRNPLVEDVFEMGSTIKPLTVASGLDAGVIAADSTYYDTGSITLNS
                     KTISNFDGRARGTTNMQDALGNSLNLGMINIMQKLGKERFRNYMLGFGIGEKTGVDLP
                     NEVSGLVKNLNSKYEVDYATASFGQGIAMSPIEAATALSSLGNGGILVKPSVVNKVKY
                     RIGLDSSTEEEESSKRILKEKTSEEISRMLVKVVDENLLGGTYKLKNYSVAAKTGTAL
                     LVNGSGGYYNDRFIHTFFGYFPAYNPKYLVFLYTVDPQGVEYASHSLTEPFFNIAKFL
                     IGYYEIPPDR"
BASE COUNT         3510 a         2057 c         3109 g         3125 t
ORIGIN      
        1 ggcaccgtgg ttgaagttct ttccgacgaa atgggaattg tttggccgga atctatcgct
       61 ccatataagg tgcatcttat ttccctcgcg ggcaagaaag gaggggagaa ggtcaaggaa
      121 aaagccgagg aaatatacgc ttatttgacc aagagggggg tggagatact ttacgatgac
      181 cgagattcta gtgctggtga gaaatttagt gattctgatt tgattggaat accaaaaagg
      241 attattgtaa gccagagggg tatagaggag ggcagttttg agatgaagga tagaaagacg
      301 ggagaggtga ccaaggtcac agagaaggag ttgtcggaga tgtttaaatg atcaagaaat
      361 tttacaaata tttttcaagc gatatcggga ttgatttggg tactgctaac accttagttt
      421 ttttgcgcga tcacggcatc gtaatagatg agccgtctgt cgtcgcgatt aatcaaaaaa
      481 caaatagagt tgtcgctatc ggtaaggaag cgaagatgat ggtcggtaga acaccgcatc
      541 acatcaccgc agtcagaccg cttgttgatg gagttatatc tgattttgag atcacagaag
      601 agatgatcgg atattttctg aggaagactc aggaaatctc aaacaaaaaa tcacttagac
      661 cgagggtcct catcggagtg ccttccggca tcacaaacgt ggagagacga gctgttaggg
      721 acgcggctat gaattccggc gcgagagagg tccatctgat tgaggaaccg atgtctgcgg
      781 ctatcggtgt aaaattgccc gtccatgagc cgatgggaag tatggttata gatataggag
      841 gggggaccac ggatatcgcc gtcatttctt tgaacggcat agttcagtct aaaaatttaa
      901 gggttgccgg agataaattt aatcaggata ttatttctta cataagagac gagttcaaga
      961 tattgcttgg agaaaaaacg gcggaagaca taaaaatagc cataggttcc gcctgcgagc
     1021 aggactcccc gatggaatat attgtaaaag gaagagattt ggttacggga cttcctagag
     1081 aggtggttgt cacagatcac gatgtcagag aggcaatgtc caactccttg tcgttgattg
     1141 tagattctgt taaggaggta gtagagcgaa cccctccgga aatagtcgcc gatgtgatga
     1201 ataagggagt tgtattggcg ggaggaggat caatgataag aggccttgcc cacgttttgc
     1261 aaaaggaagc aaaaattccc gttcatatgg ccgaggatcc tttgcgggcc gttgttaggg
     1321 gcacgggcac agtcctcgaa aactttgatt tatatgaaga agttttaata gacaacgaag
     1381 atgagatacc tccccaataa aggaaggggt gaaaataaaa tcttttctta cttggcggcg
     1441 atcatactgt ccgtcgggtg tttgtatatg tggtattccc cgataacttc cgctttatac
     1501 agcgcgggcg tcttcataac cgcgccattt tggagtatgc aggggtatct tagcgacagc
     1561 cttgcttctt ttatgattaa ttttgacagt aaaaaagctt tgatagaaaa gaatgattat
     1621 ctcacaaaac aattaaacag ttttaaatct accgcgctag aaaataagct gatacgagat
     1681 gaaaatattg agctcaagaa aattcttggg agagaagaac cttcggcttc gggcgagaag
     1741 ccggctgctt caaagaccaa aaagctatta gcttatgtgc tgtcgggccc ggtcactcct
     1801 ccatacgata gccttataat tgatgtcggt agcgatatgg gtgtggcgca gggggacctc
     1861 gttttaaacg acgataatta tgttatgggg gaggtcgtcg aagccacctc ggggacatcc
     1921 aaggttcgtc ttttgtccgc atatggccaa aagaccggcg ttctcttggg cgacaataaa
     1981 acgatgatag agatggtcgg ccaaggtagc gggaattttt ttattgcggt gccaaaagag
     2041 tttttggtaa aagagtctga ttcggctatt ttccccggtc ctgattttaa gatggttgca
     2101 aacgttgaaa gcatagaagg taaagacggt gagatgttta agaatgtcta tctgagtgct
     2161 ccgtttaaca taaaagaaat tcgtatggtg gccgttgtca agaaaataat ttagtttaga
     2221 tgcttgccag aatatttacg gatatattta ttttttttgc cgcgatactt attccggggt
     2281 ggactttttt tgtattagga gcaatcgggg cttatttttt ttataatttt tacgaattta
     2341 ttataatttc gctgattgta gacgcggttt acggctttcc tataccgggc tggtatggat
     2401 ttcgttttgt tttcaccact ctggctattt gtgtgtttgt aataataaaa atttttaaag
     2461 taagagtcag ggcgttctag tttttgaaaa attcgcatga gaatcaataa tcgttttaat
     2521 aaagacatcg agcccgacga gatttttctc gattccacta atctgtcttc tctcaacacg
     2581 caacagtttg aagggtggat agagaaacct atatcaaaaa gagtaatcac gctcactttt
     2641 attttttttg cggcggttgt ctgtatattt tcgtggaagg tttggtccct tcaaattaaa
     2701 aacggggaaa gtttggccaa aaggagcgag agaaatagcc ttaaaaaagt gtccttccct
     2761 ccggagaggg gaattattta tgacagaaac ggaaagatgg tggcgtggaa cgacaaagat
     2821 gccagatctt attatgccga accgggactg tatagtattt tgggatattt aggttatccg
     2881 accaagaaag accttgagac agtagagaac ccgaaagaaa gattgggcag agagggggta
     2941 gagaagatat ttaacgaaat acttaggggt caggatggag taaagatggt tgaggtagat
     3001 gcgttggggg aggtgcaatc tgaaagtgtt tataaccccg gtgttaaagg gggcgacata
     3061 tatttgtcct tagacgcgga aatgtccagt gtgtggtata aggctattcg tgatttggcc
     3121 gccgataagg gattccaggg tggggcgggc ataatgatgg atataaagac aggtgaggtg
     3181 atagcgatga cgagtttccc cgagtatgac tctaacgcaa tcgtgaggaa ggacgaggca
     3241 aaaataaacg agtatctgaa agacaaaaga atgccgttct taaatagggt aaccttgggg
     3301 ctttatactc cgggatctgt tatgaagccg atctttgcct tgggggcgct taatgaaaat
     3361 attattgacc ctaagaaaga gatatacagc gccggattta tttctattcc taacccttat
     3421 tttcccgaca agccgacgat atttaaggat tggaaagcgc acgggtgggt ggatatgcag
     3481 agggccatcg ccgtgtcgtc ggacgtgtat ttttatgaga tagggggtgg ttatgaaggg
     3541 caaaaaggtc tcgggatttc aaatttggaa aaatatgcgc gtatgtttgg gattggcaca
     3601 actaccggtt ttgatatcgg gcccgaagaa aacgggaaca ttccgtctcc cgcatggaaa
     3661 gcggaaaatt ttgacggaga gaagtggagt ataggaaata cttaccacag ctcaatcggc
     3721 caatacggtt ttcaggtcac tccgatacaa atggtgcgcg cgatagcggc catcgcaaat
     3781 gacggacact tggtcgtgcc gactattcta aaggctagca actctgactg gggcagggtg
     3841 tcttctgtca tcgatattga taaaaataaa ttcgatattg ttaaagaggg gatgagaagg
     3901 tctgtcacag aaggcacctc tatgggtata agttttccaa agccgagaat tgcgtcaaag
     3961 accggaacgg ccgagcttgg cgcgcttaaa caatacgtta atgcgtggaa tgtcgggttt
     4021 ttcccatatg agtcaccaaa gtatgccttt gctgttgtaa tggagagggg gccggtcaaa
     4081 aataccgtcg gaggtgttta tataatgaat cagcttttta attggatgat aattcatagg
     4141 cctgaatatg tatcccccac cacttctgaa ataattaacc cgtagtaggc tgtataaaaa
     4201 agagctattt ttgaaattta tctatataat taaacatatt atcagaagtg gtgggggatt
     4261 gacttagagc cccaaaacat ataatcttag acattatgac aacaaatatc caatatttaa
     4321 gccaggaaaa gatcaatgaa ttaaaggcgg aattgaacga tcttaagaca aaaaagagga
     4381 aagagataac cgaaagtttg gagttcgcaa agtctttagg agatctttcg gagaacgctg
     4441 aatatcagga ggcaagagaa atgcaggcca acgtagagca gagaatttct gatctcgagg
     4501 ctattcttaa ggacgctatt attgtttcta ctaatcacaa ggacattgta gggatgggtt
     4561 ctactgtcat cgtaaaggtt gagccgggca aggaaacaaa agaatttcag gttgtcggtt
     4621 cagaagaggc caacgtttta gtcggtaaaa tatcaaacct ttctcctctt ggaacggcgt
     4681 tggtggggaa gaaggtagga agcactgtca cggtgaatac gccgaaaggg gcggtgcaat
     4741 ataagatact cgaggtaaaa taaaattact cacgcgcttg gcacataact tcagagaaag
     4801 ttaaaaacac acccgcccaa agcgggcgtg ttttttttgg tttaatttgc taatatggta
     4861 tctattaaga attcgtaatt gataatttgt aatttataat ttaccctatt aagtaaaatt
     4921 ttttttaaaa atttaaaatt tgttagggct agggagtata cattatcgag ggggttgaat
     4981 aattagtaac aataaacata agtaaaaaca attaaacaat tttttactta atagggtaaa
     5041 tggcttccat agaagaaata cgagaagatc gaataaaaaa attaaatatt ttaaaagaaa
     5101 aggggataga tgcttatccg gccaggtcgc atcgtgattt ttatatctca gaagcgatag
     5161 agaggtttga tgagctggcg aagaaagaca gcatttttat ggtgggccgg gttatggctt
     5221 tgcgcggtca gggtgcgctc attttcttta atttttacga cggcacggcg acattccagg
     5281 gtcttttgaa gaagggggat attgatgact ccaagctcga tctttttaat atcacgattg
     5341 atatcggtga ttttattgaa gtgttcggaa atctttttgt gacaaaaaga ggggagaaaa
     5401 cgatacaagt aaaagattgg aggatattgt ctaaaagctt gcgaccgctt ccgggagaat
     5461 actatgggtt caaggatgag gaggagaggc ttcgaaaaag atatcttgat attttggtca
     5521 acgaagacat taaggatcgc gtattgaaga ggaccaagtt ttgggcttct attaggaatt
     5581 tcttagtgaa caaggggttt gccgaagttg agactcctgt ccttgagaat actgccggag
     5641 gggcagaggc aagacctttt ataacccatc acaacgccct tgatatagat gtttatctcc
     5701 gaatttccgc gggagagctt tggcaaaaaa gacttatggt cgccgggctt ccgaaggttt
     5761 ttgaaatagg aagaattttt agaaatgagg ggatggatgc tgaacacctg caagattata
     5821 cccagctcga attttatcag gcatattccg actacaacga ggggatggag atgataaaag
     5881 agatgtatcg atacgttgcg aaagaaactt tcggcacatc aaaatttaat ataaaaggat
     5941 ttgagatcga tctcgacgat gattggaaaa cttatgatta tgtagagacg atcaagaaaa
     6001 tgataggcgt tgatatctgg accgcggagc tttctgatat ggaaaataaa ttgcgcgaat
     6061 tgaaggttga atatgacgcg aaaggattta acaaaacaag ggccatcgat aacttgtgga
     6121 aattctgcag aaagcagata ggcggtcccg gatttttgat caatgttccg gtatttatgg
     6181 agcctctcgc aaaaagaaaa gaaggcgacg atagggtcgt ggaaagattc caggtgattc
     6241 ttgccggctc agaagtcgga aaaggtttta gtgaattaaa tgatccgata gaccaggaag
     6301 agagatttat ggagcaggca acattgcgcg aaggagggga cgaggaagct cagatgcacg
     6361 accacgatta tgtcgaggcg atggaatatg gcatgccgcc gacattcgga ttcggagtca
     6421 gcgaaagact ctttgctttc ttcgttgata agccggcaag agagtgtcag atattcccgc
     6481 tcatgagacc aaaagaagaa taaagaatta agaattatta atcgtgcatt ctctatgccg
     6541 tcattgcgag ggcgaggcga cttagccgaa gtccgtggca atccaaaatt acgaattaga
     6601 aattaggaat tacaaccatt cattctattg gttgtcattg cgagcgaagc gcggcaatcc
     6661 agaattaaaa ttgtgaatta aaaaatcgcc gtcccgatgg gacggcgatt gctgcgttgc
     6721 gctagaaggc gggggaggac tggaagcagc gcacgatgaa ctcatgggtt acgcgatatt
     6781 tgtcgtcgtc gcccaggatg accatgttgc cggggttcct gtggtcgaac ccgcagagat
     6841 cgtggaaata gaagtcttta ccgatgaatc cgtcaatttc ttcctgggtg aatggtaacc
     6901 agccacccct cgtctggcag aggcgcacga tgagtgcggc tgaactctcg ggttccgcac
     6961 gaccaaacgc agagaagaaa tgccgtttcg tattgatgtg cttcggtcgg atgggatagt
     7021 ttcgtccctc tttcgtggtg ccgtcgatga tcattgaatg actcctttct atgattatcc
     7081 gggttcactc cttcgtgtaa tttatctatc gcacatatag ccgaccatga ttatagaaac
     7141 cagtgcgagt ccggccctgt cgtagtttcc gtgacaggtc tcgatgacca tgagcgtgaa
     7201 gagggcaatc tcttcggcga cgtaaaagat tttgttgagc agataccgac ccatttgcgt
     7261 ttgcgtgggc attgagttgc tcacccccct ctctttattt aatgttcaga tgtctataat
     7321 tcaatctaat aataaataca gcaactgtca atttttgtgg tattaaaaaa tcgccgtccc
     7381 gatagggcgg cgattgctgc gaagagcggc ggtttagtcc gtcatgccac tgagcctgcg
     7441 gtgtgctccg atggtgatgg agctgaaccc ccagacgaaa accaggagcc gtcccactcc
     7501 cggaggaaga ctgttggaca gggcggcgat catcagaacc agggcgagga gcatgtggag
     7561 aaatccgtac ggtttcatgt ggaccttcct tccttggtgt atcgccgtct tgatggggcg
     7621 gcggttggcg cgctagtggg tgttcatata cttgaactcg gcgcgggcga tgaagtagcc
     7681 gaggaagagg cccgcgagtc ccatgatgcc gtctcccggg tagtacgtgg tggttgacag
     7741 cacggattgc acccagatga gaccggcggc cagaaggcag ttgatgatga agaagaaaca
     7801 aagcgtcttg ttcatgtttg accctccatt ggccacgatg aggcgttttg cgcccgattg
     7861 aaatttaagg aactgtgaac tttttaactc ttttttaagt gtagcatata tcgtgtaaaa
     7921 agtcaaggtc ttggtaaaat gggattattt taggccccag taccagctct cgttttgcgt
     7981 ggctttgcct gcaccgttag aagtctttag gcgagcggaa tttatccaca caggggagcc
     8041 gtaggcgggt tcgcggcttg aaaaataagg agccttgaca gtttgctgga cttctaatgg
     8101 tgtggataac tgtgtttgcg tgtgttatgg aagtggtata cactatacat gtagtggtat
     8161 ggagtggtat aaacccggaa atactaccta aaaaataaaa tgcttatagg agaacacaaa
     8221 cacacgctgg atccgaaaaa aagagtttct ttgcctgcta aattcagaaa agaaatgggt
     8281 aaagaagttg ttcttggaat aggtcttgat aagtgtctct ctgtatacac agttgccgag
     8341 tgggagaagt attccgccag actttctgaa atgtctatgc ttcagtcaga taaaagaagt
     8401 ttcaacagac ttatcttagg tagcgcgtcg cttgtagaag ttgactctct tggaagaatt
     8461 ttgatttccg atgttttgaa ggaatacgcc ggcttgaaag aaaaagttgc gattattggt
     8521 gtccgttcaa atcttgagat ttgggacgag gatgcttgga acgaatacaa aggaaaagta
     8581 gccaagaatg ccgatgtgtt ggcggagaag ttgggtgagt taggcgcctt ctagctcatg
     8641 gcgcacatac ccgttctttt aaaagaggtt atagaaggat tgaatttgaa gccgggaatg
     8701 gttgtactag acgccacgct tggtggggcc ggacacagca aagagatgtg taaacttatc
     8761 ggcaaggaag gcttgcttgt cgggatagac gaggacaagg ccgctatagg aagagcggag
     8821 gtagcgctaa aagatctttg caggtttaac ttgttccttt ccaacttcag agagctttct
     8881 actgtattgc agagtgcagg ggttccgcag atagatgccg ctttgtttga cctaggactt
     8941 tcatcatttc agcttgaaga gtcaaaaaga ggttttactt ttttgaaaga cgaaccgctt
     9001 atgatgactt ttgaagaggc gccgagcgaa gataaactta ccgccggaga aattgtgaat
     9061 gaatgggccg aagagagtct cgcggacatt atttacggat atggtggaga aagatacgca
     9121 agaaggattg cgaaggggat agtagaggcc agggagaaga agccgataga aacgacattt
     9181 gaactggtag agataataag aaattcggtc ccggtaattt atcagaaaag aaggatacat
     9241 tttgcgacaa agacattcca ggcattacgt atcacggtta atgacgaaat aggagcagtg
     9301 aagaaggcac tagaggatgt gtggggcaag gtgaaggtag gcggcagggt ggcggtcatc
     9361 tctttccacg aaatagaaga cagggcggta aagacatttt ttaagggaag gaaggagttt
     9421 ggagacgcga agcttataac gaagaaaccg atagtcccgg gagaagatga ggtcttggaa
     9481 aacccaagat cgaggagctc gaagctccga atagcggaaa aaataaatgc gatcatctag
     9541 cctaaaaata aataaaaata attttgaaat aggaaaattc cgcggagttt tgttttgcgc
     9601 catgatgcta ctttccccga tgtacggcta tcttgttttg agctccgttg taaatatttc
     9661 ccagagagaa aaattgagtg atgagatctc cgctataaca gccaaggtct cggtacttga
     9721 agcaaattat attacaatga gaagtgccat aaacatcgat gtcgcgggca agcttgggat
     9781 gaaggaagag ttcaacaaaa taaatttcgc aagcgccgac aagggcgatg ttaagggagg
     9841 cctttcttat gtcaataatg aaatatgatc actctcctag aataagtttt ttggtttatg
     9901 cctcagttct gtttgccgcg atacttatag cgcggctttt tgaaattcaa atagtaaggg
     9961 gcagtgatta cagcgaaatg gccgctagac agtattcgag gccaatgccc gatattttct
    10021 caaggggcac aatatatttc acggaaaaga acggcaatct ggtttcggcc gcgaccctgc
    10081 agacaaattt cgtcctggct gttaacccgg aggctataaa agatgcgggg gccactttta
    10141 aaagcttatc tccgttcgtt gatttcagcg aggaagagtt tctgaagcga gcttctaaag
    10201 aggatgatac ttatgaggtt gtgggaaaaa taaaagggga ggatgagttt gaaaagatca
    10261 atgaacttaa attggcgggg gtattcacat ttagagaaaa atcccgtttt tatccggccg
    10321 ggagactggc ttcacacgtg ctggggattg tctccagaag caaggaggaa ggtgagaggt
    10381 atatagggcg atatggcgcg gaaaagcagt atgaggacct tttgcatagg gatagcgagt
    10441 cagtatatgt aaatttcttc gcggaaattt tttcaaatgt taagaaaacg atattaggcg
    10501 acggtaataa tctttccggc gatgtggctt taagtatcga gccgacggtg gagggcgttt
    10561 tggaaaaaga gctcgtgaat atacaagaag catggaaagg aaaatcctcc gcaggaataa
    10621 taatggagcc gaagacggga aaaattgttg cgatggcagc cctgccggat tttgatccta
    10681 actattttgg aaaggaaaaa agcgtttctg tttttagaaa tccactagtg gaagatgtgt
    10741 ttgaaatggg ttctaccata aagccgctca ccgtagcttc cggattggat gccggagtta
    10801 tagcggcaga ttctacatat tatgataccg gctcgataac attaaacagc aagacgataa
    10861 gtaactttga cggcagggcg agaggcacta cgaatatgca agatgctttg ggcaattcgt
    10921 taaacctggg aatgataaat attatgcaga agctcggcaa ggagcgtttt cgaaattata
    10981 tgctcggatt tggcatagga gagaagacgg gagtagattt gccaaacgaa gtatcgggat
    11041 tggtgaaaaa cttaaatagc aaatatgagg ttgactacgc tactgcgtct tttggccaag
    11101 ggattgcgat gagccccata gaggcggcta ccgccctctc ttctttaggc aacgggggaa
    11161 tattggtgaa gccatccgtt gtgaataagg ttaaatacag gatcggcttg gattcctcaa
    11221 ccgaggaaga agagagtagt aaaagaatat taaaagaaaa gacctctgag gagataagca
    11281 gaatgcttgt taaggttgtt gatgaaaatt tgctcggtgg aacctataaa ttaaaaaatt
    11341 attctgttgc cgcaaagaca gggacagcgt tacttgttaa cggaagcggt ggttattata
    11401 atgacagatt cattcatact ttttttgggt atttcccggc atataatcct aaatatctgg
    11461 tatttcttta caccgttgac ccgcaaggag tagaatacgc ttcacacagt ttgaccgagc
    11521 cattttttaa catagccaaa ttccttatcg gttattatga aattcctcca gatagatagt
    11581 gattcgtgtc atccccgcgt aggcggggat ccagagtcaa ctatggattc ccgttttcac
    11641 gggaatgaca aataagtaga gaaaagttgg aatttatgaa atcaattttt aaaaacataa
    11701 tagtttcaat aataacccta gaggctaagt tcgttcttaa aaaatataag ccgagaatag
    11761 tcgcagtgac aggtagcgtg ggcaaaacat ctacaaagga c
//
LOCUS       DAOTEX010000022        11210 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3118
            NODE_4098_length_11210_cov_3.845361, whole genome shotgun sequence.
ACCESSION   DAOTEX010000022 DAOTEX010000000
VERSION     DAOTEX010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868142
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 11210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 883
            CDSs (total)                      :: 833
            Genes (coding)                    :: 829
            CDSs (with protein)               :: 829
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11210
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4098_length_11210_cov_3.845361"
                     /isolate="AMR_MDS_3118"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(311..1717)
                     /gene="secD"
                     /locus_tag="P5056_02040"
     CDS             complement(311..1717)
                     /gene="secD"
                     /locus_tag="P5056_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HRY62528.1"
                     /translation="MLKTRLSATLLLLVGILLGYAVYSGEVNKDSYFAKYPFKLGLDL
                     RGGVHLIYKADISKLAESTDVSSAMSALKDVIERRVNIFGVSEPVIQVEEKSALGGGK
                     EYRLIVELPGITDVAEAIKMIGATPLLDFKTERPAAERDAILAEVKKFEAAQAKVKET
                     GQFDLTGINMELAVKDPYYVDSELNGRHLKSAELQFQQNSLFPTVGIEFNAEGEALFA
                     KLTKENVGKTIAIYLDGQPISAPVVKEEIKSGKAEISGNFTREEAKTLVGRLNSGALP
                     VPIELLSTQNISAPLGNKLLNDDLRAGIWGTLAVAVFLILWYRLPGLIAVVSLAIYII
                     FTLTVFKLFGVTMTAAGIAGVIMSIGMATDANILIFERTKEELKKGRTVSDAVKEGFD
                     RAWTSIRDSNISSMITAAILFWLGTSLIRGFALTFGLGVLISMFTAITVTRTFLYSFR
                     IKDSKLTRFLFSNGSHIY"
     gene            complement(1805..2713)
                     /locus_tag="P5056_02045"
     CDS             complement(1805..2713)
                     /locus_tag="P5056_02045"
                     /inference="COORDINATES: protein motif:HMM:NF014881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 5,10-methylenetetrahydrofolate
                     dehydrogenase/5,10-methenyltetrahydrofolate
                     cyclohydrolase"
                     /protein_id="HRY62529.1"
                     /translation="MKEGGKCGSIGYLKQSMIISGNKISEDMRIALKERVSSLCADNK
                     CGEISLGIVLVGDNAVSKKYIKKKKAFGESLGVKVNLFVFEPTIEENKLEEEVMKISS
                     THSGVVVQLPLPKNLNIQKILDAVPRQKDVDVLATTSYELFSRGEWFLPPVVRAIKEI
                     LNRSGVSDFEGMSALVLGQGRLVGKPITAWLKHEGANVMTAKKDTQDIATLSRGADLI
                     ITGVGVPGVLKLEMLPGVDDANGGKGVIIVDAGTSEASGKLMGDADPACAEKSYIFTP
                     VPGGVGPVTVAALFSNLVDLWSRDNL"
     gene            complement(2726..3982)
                     /locus_tag="P5056_02050"
     CDS             complement(2726..3982)
                     /locus_tag="P5056_02050"
                     /inference="COORDINATES: protein motif:HMM:NF024241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRY62530.1"
                     /translation="MAEITGDRDLLLNGMQEKEEKKILGFNKNIFTMGVVSFLNDVSS
                     EMVFSYIPIFLTTVLGASVTFVGLIEGLADATASILKIFAGRLSDKIRKRKALIVFGY
                     SLSALAKPILAVAMAPWHVLVVRFVDRVGKGTREAPRDALISSSVSNGDVGRAFGFNR
                     GADRLGATVGLVVAFLILPLINNNYRVLFLLSFVASAVAVLVLATFVKEIPPGAKRDG
                     EVDIKFEFKHLGVPFIVFLIASTIFALGTVSEALIILRASGIGVPLHLLPIIYLVFNL
                     TYSILSVPAGSLSDKIGHRNTYMIGMLIFSVAYVLFAKVTSSFAIWFVFAFYGIYAAF
                     TDGVGRAIVADLVQEDRLRATAYGIYNALTGLALLPGSLIFGYIWDKFGPEMAFYYGA
                     LLGVLSFIIFAFLRYDDSHVAKRLTK"
     gene            complement(4142..5212)
                     /locus_tag="P5056_02055"
     CDS             complement(4142..5212)
                     /locus_tag="P5056_02055"
                     /inference="COORDINATES: protein motif:HMM:NF013530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRY62531.1"
                     /translation="MVKPKKNIKISSNADIYTIPSRILVFGAAGYVGRMVCDLFCSSP
                     DVREIIAVDAMPMPELLKRKKKITWIEADLSKDGWQELLKDSVPDVAIHTAWDLNENP
                     DSIKNFEASEKIFKYCFTFFPLKKIIYLSSILPYGAEETNDEEKRFSEGEIFRESEYS
                     YVVLRKETEALLAKLYSISNRTKSVFVLRPGMISGPMGVSLRLQREPVLRVLDVLPYV
                     FDTGSNYGLQYVHEDDLLDLLAVLVFNKVPFKGYEVFNVVAEDVVDIKDIASYTGKSI
                     FKLPAFFFSLFLALFGSRFSEGASKSGIWRYLSYPVFADGSKLAKKLKFELLYSAKEA
                     LLGERGRYDYLIQKEDKKEDNL"
     gene            complement(5326..6642)
                     /locus_tag="P5056_02060"
     CDS             complement(5326..6642)
                     /locus_tag="P5056_02060"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HRY62532.1"
                     /translation="MTLVGKKDAIFLLLGDILSLILALWVALFIRYQQVPDLTSFETN
                     FFAFLPVFGFWMLSFFIFGFYDKQTTALKKSLPTAIFNVSVINGFVALTFFYLFAFPE
                     VTPKTNLLLCLIFSTAFVIFIRTNIFDLVRSSKIGNVILVGDSDEIREIGEEILKNKN
                     YGMNPIVLNNLDENIFQFARDNKSGSIIVNLRCGGNNKNSAIIYRLLSSDFNIIDAER
                     VYESLFDKISLSCVNDEWFLENNPDRPRFLYDFIKRSFDLVTSFILAIFSLIFYPFVY
                     LAIKLDDGGPVFLYQERVGKNGRLVNIIKFRTMSVSDEGKWVVKNDPRITRVGGFLRK
                     SRIDELPQLWNVLRGDVSLVGPRPELPKLVDLYEKEIPYYSMRHLVKPGLSGWAQIHH
                     EKPPHSIEETREKLAYDLYYIKNRSLALELEIALKTIKTLLSRSGV"
     gene            6791..7933
                     /locus_tag="P5056_02065"
     CDS             6791..7933
                     /locus_tag="P5056_02065"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRY62533.1"
                     /translation="MAKKIKIMYAVTKSVFGGAGKYVLELATSLPKDKYEVVVLFGGA
                     GILIDKLKKEGVRTISVPSLEKAIDLENDINSFNEILEIFRKEKPDIVHLNSSKIGGM
                     GALAARISGVPKIIFTAHGWPFNENRNVFSKVAIWLASWITVLLSTKTIVIDEHDLRQ
                     GKRLPFCRHKYSLIHNGIKAFEYFEKDAARDYLVQKIGTDPFEGRISIGTIAELTENK
                     GINYGIEAISMLAPALKKKISYTVIGEGDSREKLEKLIEEKGLKGKIFLPGFMDNAKQ
                     YLAAFDIFMLPSLKEGLPYVLLEAGYVGLPIITTKVGGIPDIVGDEKTDFLVRSKRAD
                     ALARAMTELIVNKKVRTALRKKIKDNVVARFNFELMREKTLKVYGA"
     gene            complement(8091..9233)
                     /gene="rodA"
                     /locus_tag="P5056_02070"
     CDS             complement(8091..9233)
                     /gene="rodA"
                     /locus_tag="P5056_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HRY62534.1"
                     /translation="MFNSDKKGFFQSFFDVDWILVLALIPILGAGLLTMNSFVGDNYF
                     AERQVVWIAISFIVFLVFSQIDWRFLKDSKLLVSLFIIACSVLLVLFLGNRIKGARSW
                     IEIASISIQPADFTKILLILILSKYFSKRHMEIASIKHLVISGTYMFIPFVLIFFQPD
                     FGSAMIIFLIWFGMIVVSGVSRKHLLWIFSLGAIAFAILWAFVFLPHQKQRIVTFLDP
                     MSDIRGAGYNAFQSQIAIGSGQVFGKGVGFGTQSRLNFLPEFETDFVFAAFAEEWGFI
                     GVMALFFLFCVIFWRILLVGFVASTNFETLYSLGFAIFIMSQFIINVGMNIGLLPVTG
                     LTIPFMSYGGSHLLTEFGGLGILMGMKKYSRATHRDNMKNEFLGPA"
     gene            complement(9233..10018)
                     /locus_tag="P5056_02075"
     CDS             complement(9233..10018)
                     /locus_tag="P5056_02075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62535.1"
                     /translation="MSWSLKRQLLIVFLLLSIFGGIAGAYYYFFVKAPISCSDGKQNQ
                     GELGIDCGGPCSAICENEILPLTIEWTRPFRLIDGKYDVASLVVNRNGAFGIPVFGYK
                     FSVFDERNVHVTDRGGSTFINPNEKFLIFSSNLDSGQRTVARAQLEFNENKKEQGWVR
                     VGDISQKPRLSVENEKMVEGDTPRLYADIVNSSPYDIKNIEVSAVVYDENDNAMAVSN
                     TMLESINKDSRGQVTFTWPAPFGAKWKRVDVLPRIDYVSTNNR"
     gene            complement(10105..10446)
                     /locus_tag="P5056_02080"
     CDS             complement(10105..10446)
                     /locus_tag="P5056_02080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62536.1"
                     /translation="MIFRLRGEISYEAGSCADTKIREFDRKFQRDSVDEAIEHAKELV
                     REFFELYKNRIDLIGEIILFGYGYGETQKIYRFRLMGEIEPEPAKPAIHIPAKKAKPG
                     KPARIIGKKIK"
     gene            complement(10559..10667)
                     /gene="rrf"
                     /locus_tag="P5056_02085"
     rRNA            complement(10559..10667)
                     /gene="rrf"
                     /locus_tag="P5056_02085"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(11008..>11210)
                     /locus_tag="P5056_02090"
     rRNA            complement(11008..>11210)
                     /locus_tag="P5056_02090"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         3499 a         2614 c         2067 g         3030 t
ORIGIN      
        1 gaaagtaatt ttatccggtt gagtaaaatt agctccgcta atttaaattt tatttgaaaa
       61 ataaaatttt actcaactgg attacttaac agggtgaatt atttgaattt aatgaaatat
      121 atgagcgcta ctattaccaa aagtgcaaat gctgatgata tttccattta ccttatttag
      181 taaaaattgg ctgataatga acttttcgaa tgatagcgtt ttttatattt tccattacta
      241 tttctatttt tagccaccta accaatttta aatttttatt ttttaaaaat aaaaatttta
      301 ctgaataggg ttaataaata tgtgaaccgt tactgaacaa gaatctcgtc agttttgagt
      361 ccttaatcct gaatgagtaa aggaatgtcc tcgtaaccgt aatggctgta aacatagaga
      421 taagcacgcc gagaccaaag gtaagtgcaa atcccctgat aaggctcgta ccaagccaga
      481 aaagaattgc ggctgttatc atgcttgaga tgtttgaatc tctgatagat gtccaagctc
      541 tgtcgaatcc ttccttaacc gcgtcagaaa cagttcttcc ttttttaagt tcttcttttg
      601 ttctttcaaa aataagaatg ttcgcgtctg tcgccatacc gatagacatg attactccgg
      661 cgattccggc ggccgtcatc gtcacaccaa acaatttaaa tactgtaagg gtaaaaataa
      721 tgtaaatagc gagtgagaca acggcgataa gtccgggcaa tctgtaccaa agtatcaaga
      781 atactgctac cgccaatgtt ccccaaatac cagccctcaa atcatcatta agaagcttat
      841 tgcccagagg ggcgcttata ttctgtgttg acaataattc aatcggaaca ggaagggccc
      901 ctgagtttaa tctacccaca agggtcttag cttcttccct tgtaaagttt ccggaaattt
      961 cagccttccc cgactttatt tcctccttga ctaccggagc agatataggc tgtccatcca
     1021 aataaatagc gattgtctta ccaacatttt ccttagtcaa ttttgcgaat aacgcctctc
     1081 cttctgcatt aaactcgatt ccaaccgtcg ggaaaaggga attctgctgg aactgcaatt
     1141 ccgcgctctt taagtgtctg ccgtttaatt ctgaatcaac atagtatgga tctttaacgg
     1201 caagctccat gtttatacct gtaaggtcaa actgacccgt ctctttaacc ttggcctgtg
     1261 ccgcctcgaa ctttttaact tcagccaaaa tcgcgtctct ctctgctgca ggtctctcgg
     1321 tcttgaaatc aagaagaggg gttgctccaa tcatcttaat cgcttcggcg acatctgtta
     1381 tgcccggaag ctctacaata agccgatatt ccttaccacc gcccagagca cttttttcct
     1441 caacctggat taccggctca gaaactccaa aaatatttac ccttctctcg ataacatctt
     1501 tgagtgcgga cattgcgcta ctgacatcag tagactctgc taattttgat atatccgctt
     1561 tataaattaa atgaacccct cccctaaggt ctaagcccaa cttaaatgga tacttagcaa
     1621 aataagagtc tttgttaacc tctcccgaat aaactgcata tcctaaaagg atacccacaa
     1681 gcaacaataa tgttgccgat aatctagttt ttaacatgag gaatatatta cacgcgaaga
     1741 cactattaat caacccggcg aacaatcggg ataacattaa aatataaggt caaatatgag
     1801 attgttaaag gttgtcgcgg gaccaaaggt ctacaagatt gctaaaaagc gcggccacag
     1861 tgactggccc tacccccccc ggaacaggcg taaaaatata gcttttttcg gcgcaggcgg
     1921 ggtcggcatc gcccatgagc ttgcctgagg cctcagaagt gccagcatca acaattatca
     1981 cccctttgcc cccatttgca tcgtctacac cgggaagcat ctccagcttc aagacgcccg
     2041 gaacgccaac acccgttatt atcaaatctg cacccctaga caatgtggca atatcctgcg
     2101 tgtctttctt cgcggtcatt acattcgccc cctcgtgttt aagccatgcg gtgatgggtt
     2161 taccgacaag tcttccttgg cccagaacga gcgcggacat tccttcaaaa tctgatacac
     2221 cggaacgatt cagaatttct tttatggcgc gtaccaccgg aggcaagaac cactcccctc
     2281 ttgagaataa ttcgtatgac gttgtggcaa gaacatctac gtctttctgc cttggcacag
     2341 catccaatat tttttgaata tttaaatttt tagggagggg cagctgcaca accacaccgc
     2401 tgtgtgtaga agatattttc atcacctctt cttcaagctt gttctcttct attgtcggtt
     2461 caaaaacaaa aagattaact tttacgccca aactttcccc aaacgctttt ttctttttaa
     2521 tgtatttttt tgaaacagca ttatcgccca ccaacacaat cccgagcgat atttctccgc
     2581 atttattatc agcgcacaaa gaagaaacac gctccttaag cgcaattctc atatcttcag
     2641 aaattttgtt cccgcttatt atcatagatt gtttcaaata accaatactg ccacatttac
     2701 cgccctcttt caaccccctt agtcattatt tggtcaggcg cttcgcaaca tgagaatcgt
     2761 cataacgcaa aaaggcaaaa ataatgaatg agagaacccc caaaagggca ccataataaa
     2821 atgccatttc cggaccaaat ttatcccaaa tataaccaaa aatgaggctc ccaggcagaa
     2881 gtgcgaggcc ggtaagggca ttataaatgc cgtaagccgt ggccctaagc ctgtcttctt
     2941 ggaccagatc ggccacgatt gctcgcccaa ctccgtcagt aaaggcggca taaattccat
     3001 agaaggcaaa aacaaaccag atagcgaaag atgaagtaac tttggcgaaa agaacatagg
     3061 cgacagagaa aataagcatt cctatcatat aagtatttcg atggcctatt ttgtctgaca
     3121 gagaaccggc cggcaccgac agaatagagt atgtgaggtt gaacaccaga tagattatcg
     3181 gcaaaagatg caatgggacc ccgataccac tagcgcgcag aattatgagc gcttcagaaa
     3241 ctgtgccgag agcaaaaata gtagatgcga taagaaaaac aataaaagga actcccaggt
     3301 gtttgaactc aaattttata tcaacctctc cgtccctttt cgccccgggt ggaatttctt
     3361 taacgaaggt agcaagcaca agcaccgcca cggccgaagc gacgaatgag agcaaaaaca
     3421 aaactctata attattgttt atgagcggaa gtattaaaaa ggcgactaca agcccaactg
     3481 tagcgcctag cctatccgct cctctgttaa atccgaacgc cctgccgaca tcaccatttg
     3541 agacagacga gcttatgagc gcgtctcttg gggcttctct tgtccccttg cccaccctgt
     3601 ccacgaacct gacgacaaga acatgccatg gggccatagc cacggccagt atcggcttcg
     3661 caagagcaga gaggctatat ccaaaaacaa tgagggcttt cctttttctt atcttgtcgg
     3721 acaaacggcc ggcaaagatc tttaggatgc tcgcggtcgc gtcagcaagc ccctctatga
     3781 gtcccacgaa cgtcacggac gcccccaaaa cggtggtcaa aaatatcggg atgtatgaaa
     3841 aaaccatttc cgaagaaaca tcgttcaaaa aactcaccac ccccattgtg aagatatttt
     3901 tgttgaaacc gagaattttc ttttcttctt tttcttgcat cccgtttaga agcaggtcgc
     3961 ggtctcccgt gatttctgcc atttataagt ttgtaaaatc aacttcccgc caaaaccatg
     4021 tcgcgtcatt cttaaactgc gaccgcggct tctaacggga gcataaggct aattataaca
     4081 agcacaaacc aatcggcata atcttgccca taaaataaaa acacccccat tttggggtgt
     4141 cttataaatt gtcttctttt ttatcctctt tctgtatcaa atagtcatat ctacccctct
     4201 ctcccaagag cgcctctttg gctgaatata aaagttcaaa ttttaatttc ttggccagct
     4261 tgcttccatc ggcaaagacc ggataggata gatatctcca aataccactt tttgaagccc
     4321 cctcactgaa acgcgaaccg aacaaagcca ggaataagct aaagaaaaaa gccggtaatt
     4381 taaagatgga ttttccggtg taagacgcga tatctttaat atccacaaca tcctcagcaa
     4441 caacattaaa cacttcataa cccttgaacg gaaccttgtt gaatacaaga accgccaaaa
     4501 gatctagcag gtcatcctca tgaacatact gcagtccata attgctgcct gtatcaaaca
     4561 cgtaaggcaa aacatcaagc actcttaata ctggctctct ttgaagtctt agcgacacgc
     4621 ccatcggacc gcttatcatt cccggcctaa gcacaaaaac gctttttgtc ctattagata
     4681 tactataaag ttttgccaga agcgcctctg tctcttttct caaaacaaca tacgaatatt
     4741 cgctctctct aaatatctcc ccctccgaaa atcttttctc ttcgtcgttc gtttcttcgg
     4801 caccataagg aagaatggag ctcaagtaaa ttattttttt aagcgggaaa aaagtaaaac
     4861 aatacttaaa tattttctca gaggcttcaa aatttttaat gctatcggga ttttcgttca
     4921 ggtcccaagc agtatgtata gcaacatcag gaaccgagtc cttgagaagt tcttgccagc
     4981 catcctttga tagatcggcc tcgatccacg ttattttctt ctttcttttt aaaagctccg
     5041 gcatcggcat ggcgtcaacg gctatgattt ctctaacatc cggggaagaa caaaacaaat
     5101 cgcataccat cctgcccaca tatcctgcgg cgccaaatac caatatgcgt gatggtattg
     5161 tataaatatc ggcgttagaa gagattttta tatttttttt gggtttgacc atatgcttta
     5221 acgtaaaact aaaaactaaa aactaaaagt aacattttaa aactaaaaaa tattttaaat
     5281 tttaagatgt tatttttcac ttttcatttt acgcttttcg tttctctaca ctccgcttcg
     5341 agaaagcaga gtcttaattg tcttaagcgc aatctctagc tcaagcgcca acgatctgtt
     5401 ctttatataa tacagatcat acgctaattt ctcgcgagtc tcctctatgg agtgcggagg
     5461 cttctcgtga tggatttgcg cccaaccgga aaggcccggt ttcacaaggt gcctcatact
     5521 gtaataaggg atctcttttt cgtataaatc tacaagctta ggcaattcgg ggcgaggacc
     5581 gacaagagaa acatctcccc ttaaaacgtt ccataattga gggagctcat ctattctact
     5641 cttcctaagg aatcctccaa ctcttgtaat tctcgggtca ttcttcacta cccacttgcc
     5701 ctcgtcactt acggacattg ttctaaactt tatgatattc acgagcctgc cgtttttccc
     5761 tactctttcc tgataaagga aaaccggccc gccatcatca agcttgattg ctaaataaac
     5821 aaaagggtaa aagatgagtg aaaatatggc taatataaag gatgtaacga gatcaaagct
     5881 cctttttata aaatcatata aaaatctcgg tcgatcggga ttattttcta aaaaccattc
     5941 atcattcaca caagaaagtg agatcttatc aaaaagactc tcataaaccc tctctgcgtc
     6001 tattatgttg aagtctgacg acaaaagtcg ataaattata gcgctatttt tgttgttgcc
     6061 cccgcatctc aaattaacaa ttatgcttcc ggacttatta tctcttgcaa actgaaaaat
     6121 attttcatcc aaattattta acacaatcgg gttcattccg taatttttat ttttcagtat
     6181 ctcctcccct atctctcgta tttcatccga gtcgccgacc aggataacgt ttccaatctt
     6241 cgaggaacga acgaggtcaa aaatatttgt cctgatgaag ataacaaaag cggtagaaaa
     6301 tattaaacac agcaaaagat tcgtctttgg ggtcacttcc ggaaaagcga agagatagaa
     6361 gaaagttaga gcgacaaatc cgtttattac acttacatta aaaattgcgg tcggtaaact
     6421 ttttttaagc gccgtagttt gtttgtcata aaagccaaag atgaagaaag ataacatcca
     6481 aaaaccgaac accggcaaaa aagcaaagaa atttgtctca aatgaagtaa gatcggggac
     6541 ctgctgatat cttataaaca acgccaccca caaggccaaa ataagcgata aaatatcgcc
     6601 caaaagcaag aatatagcgt cttttttacc tactaatgtc atttaccccg ttaaatcaaa
     6661 aaaataatac caataaaact tattcacagc accataacct attatacccc atattttggc
     6721 tctcttctta tttatctggg gttgggacca ttttaaccta aaaagtgtta taattaaagc
     6781 aaatacgtat atggccaaaa agattaaaat catgtatgcc gtcaccaagt ccgtgtttgg
     6841 cggagccggt aagtatgtgc tcgagctggc cacatcgctt ccaaaagaca aatatgaggt
     6901 tgtagtcctg tttggtggag ccggaatatt gatagacaag ctgaaaaaag agggggtcag
     6961 aacaatctct gtcccgtcgc tcgaaaaggc gatagattta gagaatgata taaattcatt
     7021 caacgagata ttggagatat tcagaaaaga aaagccggac attgtccatt taaatagctc
     7081 aaagatcgga ggtatgggag ccctagccgc cagaatatca ggagtgccga agattatctt
     7141 caccgctcac ggctggccct tcaatgaaaa cagaaacgtc ttctcaaagg ttgcaatttg
     7201 gcttgcaagc tggataacgg tgcttttatc aacaaaaaca attgtgatag acgagcacga
     7261 cttgaggcag ggaaagcgcc tacccttctg taggcacaaa tactctctaa ttcacaatgg
     7321 tattaaggcg ttcgaatact tcgaaaagga cgccgccaga gattatctgg tgcaaaaaat
     7381 aggcacagac ccatttgaag ggcgaatatc tataggcacg atagcggaat tgaccgaaaa
     7441 caagggtata aattacggaa ttgaggcaat ttctatgctt gcgcccgctc tcaagaaaaa
     7501 aatatcttat acagtaatag gagaaggaga cagcagagaa aagcttgaaa aactcatcga
     7561 agaaaaaggc ctgaagggta aaatcttcct ccccggcttt atggataatg ctaaacaata
     7621 tctggcggcc ttcgacatct ttatgctccc ctctttaaaa gaaggactgc cttatgttct
     7681 gcttgaagcc ggatatgttg ggctaccaat cataacaact aaagtcggcg gaataccgga
     7741 catcgtcgga gacgaaaaaa cagacttctt ggtgagatca aaaagagctg atgctttggc
     7801 gagggctatg actgagctta ttgttaacaa gaaagtcagg acggctctac gcaaaaaaat
     7861 aaaagacaac gtagtcgcaa ggttcaactt cgaactaatg cgagaaaaaa ctctgaaggt
     7921 atacggggct taatttaggc tggtcccaaa aactcattct tccccgatag gaaagttcaa
     7981 acaacttata atctgaactt agacataacg catggaattc gcctgcggct ttttattacg
     8041 gccacttaaa taacaacctc cgaacttcct acggggcagg catattatct ctacgctggg
     8101 ccgaggaatt cattcttcat attgtcccta tgagttgcgc gggagtattt tttcataccc
     8161 ataagtattc ctagcccccc aaactctgtg agcaagtgcg aacctccata gctcataaat
     8221 ggtatagtga gacccgtgac gggcaaaagg ccgatattca tgcccacatt tataataaat
     8281 tggctcatta taaatatagc aaagccaagg ctatataaag tctcgaaatt ggtagaagca
     8341 acaaatccca ccaataaaat cctccaaaag atgacacaga acaagaagaa gagggccatg
     8401 acgcctataa aaccccactc ttcggcaaag gcggcgaaca cgaaatcagt ctcaaattca
     8461 ggcaaaaaat tcaaacgtga ctgagtgccg aaaccgaccc ccttcccgaa aacctggccc
     8521 gagccgatag caatttgcga ctggaaagcg ttgtaacctg cccctcttat atctgacatc
     8581 gggtccaaaa atgtgacgat cctctgcttc tgatgcggga gaaaaacaaa agcccacagg
     8641 attgcgaacg ctattgcgcc tagcgaaaat atccacaata aatgttttct agataccccg
     8701 gagacgacta tcattccaaa ccatatcaaa aatatgatca tggccgaacc gaaatcgggc
     8761 tggaaaaaga taagaacaaa aggaataaac atatatgtcc ccgatataac aagatgcttt
     8821 atggaggcaa tttccatgtg tcttttagaa aaatattttg aaagaatgag tatgagaagt
     8881 atctttgtaa aatcagccgg ctgtatggag atggacgcta tttctatcca gcttctggct
     8941 cccttaatcc tatttcccaa aaacagaacc aagagtactg agcatgcaat aataaacaaa
     9001 gagactagca atttgctgtc ttttaaaaac ctccagtcta tttggctaaa aacaaggaaa
     9061 acaataaaag aaattgctat ccatacaacc tgcctctccg caaaataatt gtcgccgaca
     9121 aaagagttca tggtcaaaag gcccgcgcca agtatgggaa taagggccaa gaccaatatc
     9181 caatctacat caaaaaagga ttggaaaaaa ccttttttat ctgaattaaa cattatctgt
     9241 tgtttgtgga aacgtaatct attctaggca aaacatcaac tcttttccac ttcgctccaa
     9301 aaggcgccgg ccaagtgaaa gtgacctgcc ctctcgaatc tttattaata gattcaagca
     9361 ttgtattgct taccgccatc gcattgtcgt tttcatcata aacgacagcg gaaacctcga
     9421 tattctttat gtcataaggc gaagagttca cgatatcagc ataaagtctc ggggtatcac
     9481 cttcgaccat cttctcattc tcaacagaaa gcctcggctt ttgtgaaatg tcccctaccc
     9541 tgacccaacc ctgttctttt ttattttcgt taaactcaag ctgcgccctg gcgacggttc
     9601 gctggccaga atcaagattt gaagaaaata tcaaaaattt ttcattcggg tttataaacg
     9661 tagagccccc cctatcggta acgtggacgt tcctttcgtc aaatactgaa aatttataac
     9721 caaaaacagg aataccaaaa gccccattcc tattaaccac aagcgaagcc acatcatact
     9781 ttccgtcgat aagtctaaag ggtctcgtcc actctatagt taaaggaagt atctcgttct
     9841 cgcaaatagc agaacaaggc cccccacaat cgattccgag ttcgccttgg ttttgtttgc
     9901 cgtcagagca acttatagga gccttaacaa aaaaataata atacgctcct gcgattccac
     9961 caaaaataga tagcaaaaga aaaactatca ataactgcct cttaagactc catgacatac
    10021 aattaaaatt ataaattctt aattaactta atactatcaa aaaataatcc gtataaaaag
    10081 aaaagccccg tttggggctt tacgctattt aattttctta ccgataattc gagcaggctt
    10141 accgggcttg gcctttttag cgggaatatg aattgcaggc ttagcaggct cgggttcaat
    10201 ttctcccatc aatctaaacc tataaatctt ttgggtctcg ccataaccgt aaccaaagag
    10261 gattatctcc ccgatgaggt caatgcgatt cttatataat tcaaagaatt cccggaccaa
    10321 ctccttggca tgctcaatag cctcatcaac agagtcgcgc tggaattttc tatcaaattc
    10381 cctgattttt gtgtcggcgc aactaccagc ttcgtaactg atttctcccc tgagcctaaa
    10441 aatcattcca atctccccct ttttaatgtt ccttctaata tatttaacac taaggacaat
    10501 aaaaggttgc aaaacaaaaa cacccccagt tgggagtgtt tttatattca gtgctttgtt
    10561 ccggcggctg gctactttcc cctccataag agtatcatcg ccactaagag gcttaacttc
    10621 tgagttcgga atgggatcag gtgtgaccct ctcgtcaagc caccagaaca aaacattgaa
    10681 tcgattttaa agactgctta gggcagggat tcccaagagg gaatccctta aaaatggtca
    10741 ttgggagtgg agagtttaat ccccagaacg gctctttcgg agagcgaccg agttgagctt
    10801 cagcccactc actagtcgct ttcacacaaa agccgttcct ccccgtaacc aaagtggcgc
    10861 cccatagaac ggcctatcgg catactccca gtccttggga acgtccacag gggcaagaaa
    10921 gcttaaagaa caatacccta agcaatcttc aaaatgagat ctttatataa acaatgtgag
    10981 caaaaatata cttcgtggct ttcaaatttc ctaataagaa tcgactaatt agtactcctc
    11041 ggctgaacat attactatgc ttacacctag agcctatccc catcttgcga tgagccgaca
    11101 tacgtcggac atggtcttct accatgagtc taaaacgatt tctaatctta aagtcggttt
    11161 ccctcttaga tgctttcaga ggttatccaa tccagactta gctacggagc
//
LOCUS       DAOTEX010000023        11173 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3118
            NODE_4119_length_11173_cov_4.089045, whole genome shotgun sequence.
ACCESSION   DAOTEX010000023 DAOTEX010000000
VERSION     DAOTEX010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868142
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 11173)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11173)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 883
            CDSs (total)                      :: 833
            Genes (coding)                    :: 829
            CDSs (with protein)               :: 829
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11173
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4119_length_11173_cov_4.089045"
                     /isolate="AMR_MDS_3118"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..180
                     /locus_tag="P5056_02095"
     CDS             <1..180
                     /locus_tag="P5056_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62537.1"
                     /translation="ERHEIGFDWPDTKDIFAVGDVTCKYLPGGGDIASATGANPSRQA
                     ATRINVRYFLRLNIP"
     gene            complement(270..890)
                     /locus_tag="P5056_02100"
     CDS             complement(270..890)
                     /locus_tag="P5056_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62538.1"
                     /translation="MTKSDERMNYFDNVPFLNLDYFFHAVYRVIYFVPDLVFDTILFW
                     TGITDSPFLKFIFGFLTVAALGVIFYSLYKIWNLHQAEKAAFEAIFIVEEPKKQEKKM
                     INEEWADIMGHMQSPNSSSWVLAVIESDKIMDMLLRERGFAGKDLGEMLKSDKGKNLR
                     TIQDAWEGHKIRNKIAHEAGHVLEKREAMTAIAHYESVFKELGFLD"
     assembly_gap    1074..1083
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1084..2446)
                     /gene="gatA"
                     /locus_tag="P5056_02105"
     CDS             complement(<1084..2446)
                     /gene="gatA"
                     /locus_tag="P5056_02105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HRY62539.1"
                     /translation="MNYELGKLTIKKAVEGIKAGDFSAVELTGEYLKNIENKEAEIGA
                     YLEVFDDALENAKKVDEKIKNGDKSGTLLGVPMAFKDNILIEGKTCSAGSKMLENYKA
                     TYDATVISRLKEAGVVALGRANMDEFAMGSSTENSAFKITKNPHDLSRVPGGSSGGSA
                     ASVAGEMALGSLGSDTGGSVRQPAAFCGIVGLKPTYGAVSRNGVVAMGSSLDQIGPLG
                     KRVEDVEAIFDVIHGRDEMDSTTRIYPDNGGKNFAKRIGVPRNFLQDGLSKEIEENFD
                     KTLADLKSLGYEIVDIDLPNIGYSLPVYYVIMPAEVSTNLARFDGVRYGLHKDGANLL
                     EDYVKSRGAGFGPEVRRRIILGTYVLSSGYYDAYYKKAEEVRGLIKNDFARIFNGANA
                     VSAMITPTTPSPAFKIGEKTEDPLKMYLEDIFTVPANIVGVPAMSVPSGFAKLEGKDI
                     PLGV"
     gene            complement(2452..2754)
                     /gene="gatC"
                     /locus_tag="P5056_02110"
     CDS             complement(2452..2754)
                     /gene="gatC"
                     /locus_tag="P5056_02110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HRY62540.1"
                     /translation="MIDAGTIKRLAELSRIELSPAESEKIQKDVGSILDYVSELKNAP
                     VGGIVNDKEHINIMREDSDPHESGGYTDKILAEAPKQNGGYFVVKQIMEEKKRITN"
     gene            complement(2868..4880)
                     /gene="ligA"
                     /locus_tag="P5056_02115"
     CDS             complement(2868..4880)
                     /gene="ligA"
                     /locus_tag="P5056_02115"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HRY62541.1"
                     /translation="MEDLRKVEERVKKLREVINYHRELYHVYDKQEISEEALDSLKYE
                     LATLEQKYPQFITPDSPTQRVAGKPLDKFEKVKHKVPQWSFNDAFSEEDIRDFDTRVK
                     RFLEKEGVSLREVAYDCELKIDGFKIVLEYEKGLLKTAATRGDGVIGENVTANVRTIE
                     SIPLRLKEDVDIIAEGEIWMGKKGLERINKEQIKKGEEPFANPRNLAAGTIRQLDPKI
                     VAERKLENFAYDISEASFPVPDNQIDELKRLQSLGFKVNKNFRLCKNIDEVISYWKEW
                     QKKAEKEDYWIDGVVLKVNDRHYQELLGYTGKAPRFGIAFKFRAEQATTILEDIKVQV
                     GRTGVLTPVAHLKPVQIAGSTVSRATLHNEDEIKRLDARIGDTVILQKAGDVIPNILS
                     VIKDLRTGKEKIFKMPKDCPVCGTPVIRMEGEVAYKCPNKKCFAKERRKIYYFAGKSG
                     FNIDGLGPKIIDLLADNELISSPVDIFELKEEDVEALPRMGEKSAENLIEAINKAKKV
                     ALPKFITSLGIDGVGEETAYDLAKHFGDIDKLRKATLEDLRGVYGIGEVTAESVFNWL
                     SDASNKKLLDELLERVRITKEAEVATSERGKLSGKTFVFTGTMPTLGRDEGKQMVRDN
                     GGDVSESVSPKTDYLVAGESAGSKYDKAVKLGVKVISETEFLKLLK"
     gene            complement(5045..5629)
                     /locus_tag="P5056_02120"
     CDS             complement(5045..5629)
                     /locus_tag="P5056_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly PilX N-terminal domain-containing
                     protein"
                     /protein_id="HRY62542.1"
                     /translation="MINGFLKEKIRMINHGKELFARGRSSRRESIKINSRINKKRGFI
                     LLFSLLISSIMLTAGLAISRIIIRQIYLASVQRDSQMALFAADTGLECARYWKENGPN
                     TPPECNEQVLKDYDQAGVPLLGAGSVGSSDVKFFFNLGSPSIQKTCVQVEVKNSASPQ
                     KIKAKGYNVECDISTGKPIGGRVVERELSYIYSQ"
     gene            complement(5631..6320)
                     /locus_tag="P5056_02125"
     CDS             complement(5631..6320)
                     /locus_tag="P5056_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62543.1"
                     /translation="MLNILRKKQNIPGTTLRSKRTARLVSGFTIIEALVAISILTIAV
                     TGPLSLASKGISYSSYAKDEITAFYLANEAIDVIRNIRDTNINQGKGWLRIPVKQGVV
                     ENETDITDRCGSSDGCYFDVWQSPPDLRSAFISGKELTTLKTCNTSGLVRFGYNFDWP
                     TKFSLNKDCSSDNDIDQTYFSRTITITPVDSDAEGPTEVRATATVSWVTRSGMERSVT
                     ITENIFRLNTI"
     gene            complement(6453..7106)
                     /locus_tag="P5056_02130"
     CDS             complement(6453..7106)
                     /locus_tag="P5056_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HRY62544.1"
                     /translation="MQHRKNNKKTGFTLIELMVSISIFSVVMLIVIGALLMLNDANKK
                     AQAMRAIVDNLNFATEDMTRNIRTGRDFKCGLSVANGRVNTAARDCQRSDVETGISTL
                     GFTGISTDALTQTCYVYSFRQKTDTLPGSISYGSKDIDSGRDCVEYFSSIQLSELVSP
                     EVEVAGLQFYVFNETSSGPDQQPIVILALSGDINVEQYKFKTPFNIQTTVSQRGKAF"
     gene            complement(7133..8041)
                     /locus_tag="P5056_02135"
     CDS             complement(7133..8041)
                     /locus_tag="P5056_02135"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRY62545.1"
                     /translation="MNYELRIKNTKNTQCSGFQNFPTIKNKRRVCLVSGFSLFEMIVV
                     LAVVAFVASVTFSSFPKMNSKLSLDLLTQDVALTVRQAQIYGTTVFGVGDVTSGGAGA
                     NYQAYGVSFPSPEIRGDDGNYRYTLFADVPSLNEPVTADVNSPKNRYDEYLGGANQIC
                     MNDSEACLVSVCGNPTALPFKNECLQRFIVSGRDEVSSLCLNYMNSGNKSATKDQRVS
                     ACESPTLSQTCDNTQETITKPDPGSSVDIVFRRPKLQASITAKNSACGSGGYFAPNNI
                     GIVLKSDSGDARVVVVWRNGQISIDR"
     gene            complement(8054..8857)
                     /locus_tag="P5056_02140"
     CDS             complement(8054..8857)
                     /locus_tag="P5056_02140"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HRY62546.1"
                     /translation="MDTIFYIATFILGTIIGSFLNVVIFRYNTGKTVGGRSMCLTCSK
                     TLKWNHLIPVFSYLFLLGKCAYCKAKISIQYPIIEVLTGLAFLLILQRVYLFPIAVIY
                     FAVFSILLVIAVYDYRHKIIPDGLVFAFIGLSFLRIVAEYFLGGGAFTYDFVSGLLIA
                     LFFATLWWVSDGRWMGFGDAKLALGIGWLLPFCQNLSAIMLSFFVGSIVGLFLMFVAK
                     INSRRKGIPFYFDKEIPFAPFLIFATFVSFVFNFDVLGLIMGGVIVCFG"
     gene            complement(9051..9509)
                     /locus_tag="P5056_02145"
     CDS             complement(9051..9509)
                     /locus_tag="P5056_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HRY62547.1"
                     /translation="MKKKGFTLIELLVVIAIIGILSSVVVVSLNSARAKARDAKRVAD
                     LEAVKAALSLYFDTNQAYPATLAGIGTEFLASQPVPPTANEVYLYVPSADGLSYHLGA
                     TLEQAGHTSLSADRDCSSGEVTEASPACNGFAAPATGGFAGVDPIYDVTP"
     gene            complement(9567..10067)
                     /locus_tag="P5056_02150"
     CDS             complement(9567..10067)
                     /locus_tag="P5056_02150"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HRY62548.1"
                     /translation="MSNNRKKGFTLIELLVVIAIIGMLAATVLVSLGTARAKGRDAKR
                     IADLKNIELAIQLFSDSNKGAVPATISALTPTYLAKEPKDPNTDASYAYTPCNPVAGN
                     ASKFMKYQLGADLENLAPVHEGDSDTGATGGNPALVSCTSAFTGEDESSCMGSDTNLH
                     CYDLTN"
     gene            complement(10425..>11173)
                     /locus_tag="P5056_02155"
     CDS             complement(10425..>11173)
                     /locus_tag="P5056_02155"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HRY62549.1"
                     /translation="LERSYELTSKVKNALIYPAFVIASFIIVMILMLVYVIPKLSEII
                     METGGDIPIYTKIIIGLSNFLLNYGIFIIAIIIVAGFLLARYFKTEDGEAKLSELQMN
                     TPYIGNLYKMFFLSRLSDNMDTLISSGVAMVRSVEITADVVGSNLYRDILLQVANQVK
                     AGNSLSKSLGQYVEIPQILVQMVKIGEETGNLGFVLKTMSRFYKREVDTAVDTLVGLI
                     EPFMIVALGLAVGILLVSVLGPIYNITTGI"
BASE COUNT         3129 a         2687 c         2011 g         3336 t
ORIGIN      
        1 gagcgccatg agataggatt tgactggccc gacacaaaag acatattcgc cgtaggggat
       61 gttacctgca aatatttgcc cggaggcgga gatatcgcgt ctgcgacagg agcaaaccct
      121 agcagacagg cggcaacaag aattaatgta agatattttt taagattaaa cattccttaa
      181 taaaaaatta ataaactatc acgaaaataa ttatagcatt gccctcacaa taaaaaagac
      241 cggtcttttc ggtcctttaa agctgaaaat taatccaaaa atcccaattc tttgaaaaca
      301 gactcataat gagctatcgc tgtcatagct tcacgctttt caagcacgtg tccggcttcg
      361 tgagcaatct tattccttat tttatgccct tcccaagcat cctgaatagt cctcaaattc
      421 ttccccttat ctgatttgag catttccccg agatctttcc ctgcgaaccc cctctcgcga
      481 agcaacatat ccataatttt gtcggattct ataacagcca aaacccagct cgaagaattc
      541 gggctttgca tgtggcccat tatatctgcc cactcctcgt ttatcatttt cttctcttgc
      601 ttctttggtt cttccacgat gaatatcgct tcgaatgccg ctttttccgc ctggtgcaaa
      661 ttccatatct tatacaaaga ataaaatatc actccaagcg ccgcaacggt caaaaaacca
      721 aagataaatt tcaaaaatgg actatctgta ataccggtcc aaaataaaat agtatcaaaa
      781 accagatcgg gcacaaaata aataacccga taaaccgcgt ggaaaaaata atctaagttt
      841 aaaaatggaa cgttatcgaa atagttcatc ctttcgtcac tttttgtcat cctcgacttg
      901 atcggggatc catttattta aaaacaccgg attcccgcct acgcgggaat gacacaggga
      961 gtgcagttaa tattatacta ctttctacca cctacttacc actatctact ctctattttc
     1021 tatatccttt ccgatgctga aaagtatatc ctctctaaaa tgcggagcgg tgannnnnnn
     1081 nnngaacccc aagcgggatg tcttttcctt ctaatttggc aaaacctgat ggcacagaca
     1141 ttgcgggaac acctacaata ttcgcaggga cggtaaaaat atcctccaaa tacatcttta
     1201 atgggtcttc ggtcttctcc cctatcttaa acgcgggcga tggagtggtc ggcgtgatca
     1261 tagcagacac agcatttgcg ccgttaaata ttctggcaaa atcgtttttg ataaggcctc
     1321 gcacctcctc ggcttttttg taatatgcat cataatatcc tgaagaaaga acgtaagttc
     1381 caaggatgat cctgcgtcta acctcgggac caaatccggc ccctcgtgat ttcacataat
     1441 cctccaggag attcgcgccg tctttgtgca gtccatatct gaccccatca aatctggcga
     1501 ggttcgtaga gacctcggcg ggcatgatta cgtaataaac cggcaaagaa taccctatgt
     1561 ttggcagatc aatatctaca atttcgtatc ccaaagattt caggtcagca agcgtcttgt
     1621 caaaattttc ttctatctct tttgatagtc catcctgcaa aaaatttcta ggcacgccga
     1681 ttcttttcgc gaagttcttg cctccgttgt caggatatat gcgcgtagtg gaatccatct
     1741 catccctgcc gtggattaca tcaaaaattg cctccacgtc ctccaccctc tttccaagcg
     1801 gacctatctg atcaagagaa ctacccatag cgaccacacc gttcctggag accgctccat
     1861 aggtcggttt taggcccaca ataccgcaaa aagccgccgg ctgtctgaca gaccctcccg
     1921 tatctgaacc aagcgatcca agagccattt ctccggccac ggaggcggca gaaccgcccg
     1981 atgaacctcc cgggacccta cttagatcgt gcggattttt ggttatttta aatgcggaat
     2041 tctcggtaga gctacccatc gcaaattcat ccatattggc cctgcccaat gcaacgacac
     2101 cagcttcttt gagcctggaa ataactgtcg catcgtatgt tgccttataa ttctcaagca
     2161 tctttgaacc tgcagaacaa gtctttccct caattaaaat gttatcttta aaagccattg
     2221 gcacgccaag aagcgtacca ctcttatctc cgtttttaat cttttcgtct accttttttg
     2281 cattttccag agcatcatca aaaacctcca aataggcgcc tatttccgcc tctttatttt
     2341 cgatattttt taaatattcg ccggtaagtt caactgccga aaaatctccg gcctttatgc
     2401 cctcaacagc cttcttgatt gttagtttcc ctaattcgta attcataatt cctaattcgt
     2461 aattcttttc ttctcctcca ttatctgctt aaccacaaaa taaccgccat tttgtttagg
     2521 agcctcagcc aatatcttat ccgtatatcc gcccgactca tgagggtcgc tgtcttccct
     2581 cataatattt atatgttcct tatcgttcac aatacctccc accggcgcat tcttaagctc
     2641 tgatacatag tccaaaatag atccaacatc tttttgaatc ttttcacttt ccgcgggcga
     2701 taactctatt ctggaaagtt ccgccaacct ttttatagtt cctgcgtcaa tcatttaccc
     2761 cgtgaaatta aaattaaaca cacctctaat actatacaac aagattaatc tattctaccc
     2821 caataatcaa ttttccttct atttcactgg ggtttttcta tttaacacta ttttaatagt
     2881 tttaaaaatt ccgtttcaga gataacctta acccctagtt taactgcctt atcatactta
     2941 cttccggcgc tttctccggc cacaagataa tctgtctttg gggaaaccga ttctgacaca
     3001 tctccgccgt tatcgcgcac catttgcttg ccctcatcgc gaccaagcgt aggcattgtg
     3061 ccggtaaata cgaaggtttt acctgaaagc ttccctctct cgctcgtagc gacctcggct
     3121 tcttttgtga tccttactct ttctagtaat tcgtctaaca atttcttatt tgaggcgtca
     3181 gacagccaat taaacaccga ctccgcagtc acttcaccta ttccataaac ccctcgtaga
     3241 tcttccaacg tagcctttcg caatttatct atatctccaa aatgcttcgc cagatcataa
     3301 gcagtttctt ctcccacgcc atctataccc aaagacgtaa taaacttcgg taacgccact
     3361 tttttggctt tatttatggc ttctataagg ttttctgcag acttttcccc cattctaggt
     3421 agagcttcca cgtcctcctc ttttaattca aaaatatcta cgggtgaaga gattaattca
     3481 ttgtctgcaa gcagatcgat tatcttcgga ccgagcccgt cgatattaaa gcccgacttg
     3541 ccggcgaagt aatatatctt tcttctctcc ttcgcaaaac attttttatt aggacactta
     3601 tatgctacct ccccctccat tcttataacc ggagttccgc agacaggaca atctttcggc
     3661 attttgaata ttttttcctt acccgttctc aagtctttta tcaccgacaa aatattcggt
     3721 atgacgtccc cggctttttg cagaataaca gtgtctccaa tgcgcgcatc cagccttttt
     3781 atttcatctt cattatggag cgtggcgcgt gacaccgtgc taccggctat ttgcaccggt
     3841 ttcaagtgcg cgacgggagt aagaaccccc gttctaccca cctggacctt gatatcttca
     3901 agaattgttg ttgcctgttc cgccctaaat ttaaaagcga taccaaaacg aggagctttc
     3961 cccgtatagc caagaagctc ctgataatgc ctgtcgttca ccttcaaaac aactccgtct
     4021 atccaataat cttctttttc ggcttttttc tgccactctt tccagtaaga aataacctcg
     4081 tctatgtttt tacaaagtct gaaattctta tttaccttaa atccgagaga ttgtaggcgc
     4141 ttcaattcgt ctatctgatt atccggtaca ggaaaacttg cctctgatat atcataggca
     4201 aaattttcta attttctttc cgccactatc tttggatcaa gctggcgtat ggtgccggcg
     4261 gcaagatttc ttggatttgc gaacggctct tctcccttct ttatttgttc tttgtttatt
     4321 ctctcaagcc ccttctttcc catccaaatc tccccctctg caataatatc aacatcttct
     4381 tttaggcgaa gtggtataga ttctattgtt cgcacatttg ccgttacatt ttcaccaatt
     4441 acgccgtctc ctctcgtagc ggcggtcttc aaaagccctt tttcatattc aagcacgatc
     4501 ttgaagccat ctatctttag ctcgcaatca taagccactt cgcgcaaaga aaccccttct
     4561 ttttcaagaa atcttttaac tcttgtatca aaatcgcgga tatcttcctc agaaaacgca
     4621 tcgttaaaag accattgcgg aactttatgc tttaccttct caaatttgtc taaaggctta
     4681 ccggccacac gctgtgtcgg cgaatcaggc gttataaatt gaggatattt ttgctcgagt
     4741 gttgcaagtt cgtattttag agaatctagg gcttcttccg agatctcctg cttgtcataa
     4801 acatgataaa gttcacggtg ataatttatc acctctcgta gcttttttac tctttcttcc
     4861 acctttcgca gatcttccat attgttctga ttatagtaga aaaagacaca atttcccagc
     4921 acaaaattga agacaaaaac cccggtgaaa taagtgcttg aatgggatta ttgcaaaaga
     4981 taaagtttat cttattaaaa accaccgcag tgggtgggat ttaatttcac ggggtaaata
     5041 aaatttattg tgaatatata taagacagct ctctttctac taccctgcct ccaataggtt
     5101 tacctgtaga aatatcgcac tctacattgt atcctttggc ttttatcttt tgaggggacg
     5161 ctgaattttt cacttcaacc tggacacagg tcttttggat acttggagaa cccaaattaa
     5221 agaagaattt cacatcgctg ctgccgacag acccggcgcc aagcaaagga actcccgcct
     5281 gatcataatc tttcagcacc tgttcgttac attccggagg cgtattgggg ccattttcct
     5341 tccaatatct tgcacactcg agccccgtat cagcggcaaa aagggccatc tgggaatctc
     5401 tctgcacaga tgccagataa atctgcctta ttataattcg cgatattgcg agcccggcag
     5461 tcagcataat acttgagata agaagcgaaa aaagcaggat aaagccgcgc tttttgttta
     5521 ttctggaatt tatttttata ctctcgcgcc tactgcttcg tcccctggca aaaagctcct
     5581 tgccgtgatt tatcatcctt attttctctt ttaaaaatcc gtttatcatt ttagatagta
     5641 tttaatctaa aaatattttc agttattgtg acacttcttt ccattccgct tctggtcacc
     5701 caagagacag tcgccgtcgc tctaacttct gttggtcctt ccgcatccga atccacggga
     5761 gttattgtaa tagtccttga aaaatatgtt tgatctatat cgttatctga agagcagtct
     5821 ttattaagac taaatttagt aggccaatca aaattataac caaatctgac aagccccgat
     5881 gtattgcaag tcttaagggt tgttagctct ttcccggata taaatgcgga ccgtaggtcc
     5941 ggaggactct gccaaacatc aaaataacag ccgtctgagc ttccgcaccg gtctgtaatg
     6001 tctgtctcat tctctacgac accctgtttg accggaattc taagccaacc cttcccctgg
     6061 ttgatgtttg tatctctaat atttctgatg acgtctatcg cctcattggc cagataaaaa
     6121 gcagtaatct catcctttgc atagctagaa taactaatcc cttttgaagc caaagaaaga
     6181 ggaccggtca cagcaatcgt aagaatagaa atagcgacta gcgcctctat aatagtaaac
     6241 cccgacacca aacgagccgt ccttttgctt cgcaaggttg ttccgggaat attttgtttt
     6301 tttcttaata tattcagcat ttatttttta tatattttat tttagaagca acggctcgtt
     6361 tttgttattt tatttaccct gttaagtaaa aatttaattg taattaaatt ttaaattttt
     6421 ataaaaattt tactaaacag ggtaaatcat ttttaaaacg cttttcctct ctgcgaaacg
     6481 gtggtttgaa tattaaaagg cgtcttgaat ttatattgct ctacatttat atccccgctc
     6541 agcgccaaga ttacaatcgg ctgctgatcc ggtcccgatg aggtttcatt gaaaacataa
     6601 aattgcaacc ccgccacttc aacttccgga gaaacgagct cgctcaactg aatacttgaa
     6661 aaatactcta cgcaatccct accggagtct atgtcctttg aaccatagct aattgaaccg
     6721 gggagagtgt cggttttttg cctaaaagaa taaacgtagc aggtctgagt aagagcatcg
     6781 gtgcttatgc ctgtaaatcc gagggtgctt atgccggtct cgacatcgct acgctggcaa
     6841 tctctagcgg cagtgttcac tctaccgttt gcgacggata gtccgcattt aaaatctcta
     6901 ccggtcctga tatttctcgt catatcttct gtggcaaagt ttagattatc tacaatggct
     6961 ctcatggcct gcgccttctt gttcgcatca tttagcataa gcagagctcc gataacgatg
     7021 agcataacaa cgctaaaaat tgagatagaa accatcagct cgatgagtgt gaaccctgtt
     7081 tttttgttgt tttttctgtg ttgcattttt cgcttttcgc ttagcacttt ccttatcggt
     7141 ctattgatat ttgcccgttt ctccaaacca cgacaaccct tgcatcaccg gaatccgatt
     7201 tcaaaactat accaatatta ttgggggcaa agtatcctcc gctaccacaa gcggaatttt
     7261 ttgcggttat agatgcctgt aatttcggcc ttcggaatac aatatctaca gatgaacccg
     7321 ggtccggctt cgttatggtt tcttgcgtat tatcgcaggt ttgagagaga gtcggcgact
     7381 cacaggcaga taccctttgg tctttagtag cagacttgtt gccgctattc atgtaattca
     7441 aacaaagcga gctgacctcg tcccttcctg aaacgataaa tctttgcaaa cactcatttt
     7501 tgaaaggcaa ggcagtcgga ttgccacaaa cagacacaag acaggcctct gaatcattca
     7561 tgcaaatttg attagctcct ccaagatatt catcatatct atttttagga gaattgacgt
     7621 ctgcggtaac cggctcattt agactcggga catccgcaaa tagcgtatat ctataatttc
     7681 catcatctcc ccttatttcc gggcttggga aacttactcc ataagcctga tagttggcac
     7741 ccgcgccgcc gctagtcaca tcccccaccc cgaaaaccgt tgttccatat atctgagcct
     7801 gtctcactgt cagagcgacg tcctgtgtaa gcaagtccag tgagagcttg ctgttcatct
     7861 tcgggaaact agaaaaagta acggaggcca caaaagcaac aaccgccaaa accactatca
     7921 tttcaaataa cgagaacccg gacaccaaac aaacccttct cttgtttttt attgtcggaa
     7981 aattttgaaa cccagaacac tgagtatttt ttgtattctt aattcttaat tcgtaattca
     8041 taattctttt gaactatccg aaacaaacaa ttactccacc cattattaaa cctaggacat
     8101 caaaattaaa aacgaaagaa acgaatgttg cgaatataag gaatggcgca aacggaatct
     8161 ctttatcaaa ataaaaagga attcccttcc ttctggaatt tattttcgcg acaaacatga
     8221 ggaaaagacc cacgatggaa ccgacgaaaa aggaaagcat tatggcgctc aaattttggc
     8281 agaacggaag aagccaacca atgccgagag cgagcttggc gtctccaaat cccatccacc
     8341 taccgtcaga cacccaccag agcgttgcaa agaaaagggc gataagaaga ccggatacaa
     8401 agtcataggt aaatgctccc cctccgagga aatattcggc gacaattcta aggaaagata
     8461 atccgataaa agcaaaaaca aggccatccg gaattatttt gtgcctataa tcatagaccg
     8521 caataacaag gagtatagaa aatacggcga aataaatgac cgcaataggg aaaagataaa
     8581 ctctttgcaa gataagcaaa aaagctaaac cggtgagaac ctctattatc ggatattgga
     8641 tagatatctt cgccttgcag tatgcgcact taccaagaag gaacaggtaa ctaaatacag
     8701 ggataagatg attccatttc aaagtcttgc tacaagtgag acacatagac ctacccccca
     8761 ccgttttgcc tgtgttatac ctaaaaatta ctacattcaa aaaacttccg ataattgtgc
     8821 ctagtataaa agtagcgata taaaaaatcg tatccattct taaattatat cacaggcaaa
     8881 ttaaaaaagg tctgtggata tacaaaaatc ccaccgtttt gaaggtggga tttttaaact
     8941 cattaagacc tatgtggtgc aagccctgta acaagctcta ctatcggggt ctgccctgta
     9001 gtgagctttg ctctactatc ggggcctgcc ctgtagtgag ctttgctcta ctacggggtt
     9061 acgtcgtaga tagggtctac tccggcaaat cctcccgttg caggagcggc aaaaccgtta
     9121 catgcaggtg aggcttctgt tacttcaccg gaagaacagt ctctatctgc agagagagat
     9181 gtgtgaccgg cctgctcaag tgtggctccc aagtggtagc tcaagccatc tgctgatgga
     9241 acgtatagat aaacctcatt tgcagtcgga ggaacaggct gtgaagccaa aaattctgtt
     9301 ccgatgcccg ccaaagtggc aggatatgcc tggtttgtat caaaatacaa gctgagggcg
     9361 gcctttacag cctccaagtc agctactctc tttgcgtccc tagccttagc tctggcgctg
     9421 ttcaacgaaa ctacaactac tgatgaaaga ataccgatga tcgcgatgac caccaagagt
     9481 tcaatcagtg taaatccttt tttcttcatt tttttaattt attattaatt aattttcgac
     9541 taataatttt taacaaaagc ttctagctaa tttgtcagat cgtagcaatg cagattggta
     9601 tctgaaccca tacaactact ctcatcctca ccagtaaagg cggatgtgca gcttacaaga
     9661 gcaggattac ctcctgttgc cccagtatct gaatccccct catgaacagg ggccaagttc
     9721 tctaaatcgg cacccaactg atatttcata aatttagaag cattcccagc caccggatta
     9781 catggggtat atgcgtatga agcatctgta tttggatcct tcggttcttt tgcaagatag
     9841 gttggtgtta gcgctgaaat cgttgcagga acagccccct tgtttgaatc agagaaaagc
     9901 tgaatagcta attcaatgtt cttaaggtcg gcaattctct tcgcatctct acccttagct
     9961 ctggctgtgc cgagagatac caatactgta gcggcgagca taccgatgat cgcaataacg
    10021 accaacaact caatcaatgt aaaacctttt tttctattat tcgacatttt tatttatttt
    10081 attttttaac gtattttaca aggaggcgat atcttgttca tcgaccgtgt ttgaagaaac
    10141 aaggaatcac attcctatgc tcttaatttt actaccatgc aagtgaaaag caaaatagcc
    10201 gatgtggata aataaaatac aaaatatgtc aaaaagggac ggtttttcgt cattcccgcg
    10261 aaggcgggaa tccagttttt taatagtcct tttgctcaca tggattcccg ccttcgcggg
    10321 aatgacaaac agttttgagg taaaattaag gaacccgtct tcctttttgg aagatgggtt
    10381 ccgattattg acctaattcg taattcttaa ttcttaattc ctgtctatat acctgttgta
    10441 atattatata taggccctag cacagacacg agcaatatac ctaccgcgag accaagtgcc
    10501 actatcataa acggctcgat gaggcctacg agggtatcta cagcagtatc tacctctctt
    10561 ttataaaatc gcgacattgt tttcaaaacg aaaccgaggt ttccggtctc ctcccctatc
    10621 ttcaccatct gcacgaggat ctggggaatc tctacatatt gcccgagaga ttttgagagg
    10681 gaatttccgg cttttacctg atttgcaacc tgaagaagga tgtctctgta aagatttgaa
    10741 ccgacaacgt ctgccgtgat ctctaccgaa cgcaccatcg ccactccgga agaaataagc
    10801 gtatccatat tgtctgaaag cctggaaagg aagaacatct tatacaagtt tcctatatat
    10861 ggagtattca tttgcaactc agacagcttg gcctcaccat cttcagtctt gaaatatctg
    10921 gccaacaaaa atccggcaac gataataatg gcaataatga aaataccgta atttaataag
    10981 aaattgctca atccgatgat tatcttcgta tatattggta tatctccgcc tgtctccata
    11041 atgatttctg aaagtttcgg gataacatat acaagcatca aaatcataac gattatgaat
    11101 gatgcgatga cgaaagccgg ataaatgagt gcgttcttga cctttgaggt gagctcgtat
    11161 gacctctcaa ggt
//
LOCUS       DAOTEX010000024        10667 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3118
            NODE_4372_length_10667_cov_3.884470, whole genome shotgun sequence.
ACCESSION   DAOTEX010000024 DAOTEX010000000
VERSION     DAOTEX010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868142
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 10667)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10667)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 883
            CDSs (total)                      :: 833
            Genes (coding)                    :: 829
            CDSs (with protein)               :: 829
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10667
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4372_length_10667_cov_3.884470"
                     /isolate="AMR_MDS_3118"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..742
                     /locus_tag="P5056_02160"
     CDS             <1..742
                     /locus_tag="P5056_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011283803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HRY62550.1"
                     /translation="EDEIRDLLKKQGFDDKCPVIRGSGLKALEATSLDDPWAQKVLEL
                     AKALDEYIPDPVRELDKPFLMAVEDVFSIEGRGTVVTGRIERGRIKLGEEIEIVGLKD
                     TQKTAVTGIEMFNKSLNEGLAGDNAGLLLRGLKKEDVTRGQVLCKPGSVNPHTDFECE
                     VYILKKEEGGRHTPFFNGYKPQFYVRTTDVTGDVTLPAGTEMVMPGDTVKLTVKLVAP
                     VALEDKGKFAIREGGKTVGAGVVTKIIK"
     gene            971..1270
                     /gene="rpsJ"
                     /locus_tag="P5056_02165"
     CDS             971..1270
                     /gene="rpsJ"
                     /locus_tag="P5056_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HRY62551.1"
                     /translation="MVRIRVAAYENKVLDQSVKQIIDTAMRQDAKVRGPVPLPTEIKK
                     YTVNRSSFIDKNSREQFEMRIHRRLIDILNPSSKIVEALTNLNLPSGVSVDVKMI"
     gene            1292..1921
                     /gene="rplC"
                     /locus_tag="P5056_02170"
     CDS             1292..1921
                     /gene="rplC"
                     /locus_tag="P5056_02170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HRY62552.1"
                     /translation="MYILGKKQNMTQVFDENGTCSPVTALSVGPMTVVQVKDSSTKDG
                     YNAIQFGYDEISLDKLDKAKRGHQAKAVGAKASGFRTLKELRLKGEEKSDLKVGDVVD
                     TSALNEGDKVTVSAISKGKGFQSAIKRHGFKGQPRSHGQKHCERAPGSIGGGLRNKVP
                     KGMRMAGRMGSDRVTVRGLTVVKIDKENNLVLIKGAVPGRKGTLVEIYK"
     gene            1927..2586
                     /gene="rplD"
                     /locus_tag="P5056_02175"
     CDS             1927..2586
                     /gene="rplD"
                     /locus_tag="P5056_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HRY62553.1"
                     /translation="MEAKIYNNKGVASGSVKLPEKIFGVKWNSDLVSQTLNSMRSSQR
                     AGTADAKGRGDVRGGGKKPWKQKGTGRARHGSTRSPIWVGGGVAHGPKAEKNYDRKVN
                     KKARNKALFTLLSAKFKDGELLFIDNIETKDIKTKNAALTLQNLSKIEGYNQLNYKKG
                     NRALIVTPTKSDNTIKSFRNLNTVAVEETRNMDPLTVAKYKYIIVSNPEESVKILSAR
                     S"
     gene            2593..2880
                     /gene="rplW"
                     /locus_tag="P5056_02180"
     CDS             2593..2880
                     /gene="rplW"
                     /locus_tag="P5056_02180"
                     /inference="COORDINATES: protein motif:HMM:NF012498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HRY62554.1"
                     /translation="MTTRTVLKLKKPHVTEKATMLSVNVNPAYTFEVEADINKTELKK
                     IIKEVFKVEATKINITNLPAKSVFVRGKWGTKSGMKKAVVYLKKGDKIDFA"
     gene            2884..3723
                     /gene="rplB"
                     /locus_tag="P5056_02185"
     CDS             2884..3723
                     /gene="rplB"
                     /locus_tag="P5056_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HRY62555.1"
                     /translation="MKTYRPTTPGRRQLQTIEFGKFLTASEPHKALTKGFKRNVGRNS
                     AGRITVSHKGSGHKKVYRMVDFAYSKKNIPAKIETVEYDPFRSSFISLVVFADGDRKY
                     MIVPQSMKVGDTFIISEKADIKPGNRMMLKNIPVGTFIYNIECKPEGGARLVRSAGNY
                     AEVMSNASGYINVKMPSSEIRKLDERCYACVGTVSNEEHNLVSLGKAGRTRWLGIRPT
                     VRGTAKNPCDHPYGGGEGRQGRGTKRPKTRTGKVTGGRKTRKSKKYSNIFIVTRRGKK
                     GKK"
     gene            3840..4433
                     /locus_tag="P5056_02190"
     CDS             3840..4433
                     /locus_tag="P5056_02190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62556.1"
                     /translation="MNKKFLLALALLSLAMPVAAAPFDQNRVGGADTSSAAGEDAKNV
                     KATATPEAVNQGRGEERRSEVANAVQEMLRVADRDGGIGERVREIARDQQDESVKAED
                     TLKLLKNRGQFRKFVFGSDYKKISDIEELLARHNARLAELESMLPQVKTDADRAILSD
                     KIGIMKNLGAEMQQEVDGVKDVGFSLFGWLNKMIYKK"
     gene            4443..4592
                     /locus_tag="P5056_02195"
     CDS             4443..4592
                     /locus_tag="P5056_02195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62557.1"
                     /translation="MKGKCDKCAEKPPMYLDKGKGVPGTEPCKWCERMVAPRASNKPM
                     RSKKK"
     gene            4687..5139
                     /locus_tag="P5056_02200"
     CDS             4687..5139
                     /locus_tag="P5056_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62558.1"
                     /translation="MILEANMKNKFLFLLLICAVIVPGLVTAKSENSIQKIKKPKISV
                     VNNIKKIKAVRPRNIVFNGVVKAVNGNEFTVEVAKKANSKLANVVNVQTKTQKVNLKS
                     AKVKKVNKNSVIKVGDRVKIYGIMKDANNVSEASVNVVKSPIKLKKKN"
     gene            5155..>5775
                     /locus_tag="P5056_02205"
     CDS             5155..>5775
                     /locus_tag="P5056_02205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62559.1"
                     /translation="MKNKFLKINFSKFKNFISACISILCVASFVAPNIVFAADTYFDG
                     GDGSPETPYQISTCAQLQDIGTTTEGFDPALNTLNYVLTANIDCTGTEVEGSNFIPIA
                     SFSGDFNGGGYTISNITSTYNVTNSGAGLFSSILYPANIHDLTIASSVFVPTSSGVYP
                     VYSGAVAAQMFGGTIDHVNVLSSVTLSGGENPEVSRDGTGATGGLVG"
     assembly_gap    5776..5785
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5786..9365
                     /locus_tag="P5056_02210"
     CDS             <5786..9365
                     /locus_tag="P5056_02210"
                     /inference="COORDINATES: protein motif:HMM:NF016906.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5011 domain-containing protein"
                     /protein_id="HRY62560.1"
                     /translation="ITSDAYDGSQPSIISNSTSTASFDFHNTINANGGLASYVGGLAG
                     DIRTYGNEVSISNSYANFNLEKQYGPDNYGSDVYVMSNAGGLVGRFLGANDSGDYSGS
                     LTISNSSASTTITNAYNSGGLIGAAFLYDNLSAEGGGAEFTIEDSSSSGSITADAETD
                     GKNLGGVLGYQEVAAGSVNTITNITRSHSSVSIFDSPVSDDLEVYLSCAGGLVGEVYG
                     LGSFNITESYSTGNIGSSEGSHQLGGLVGELYSQSSGGEGVNITRSYSTGDVYGYLDL
                     GGILGGTISDGASFDLSITNSYSTGDIGNGESSTNVGGIVGNGDYLKLVIDSTYASGD
                     IGLDIYASAGGIVGSINSDSSVSNSFYSPAIVLAAYYGETSSAIVGYNGGATLTGNYY
                     TNVPDLEDDGYLAGGVDSFKGNGIITNNLFDPVWDTSVTPIWVLTAGAYPTLNAIAAD
                     NPVAAPSSVYVNENYNPSYAGGLVWEVNAFNKIQRAMNAVASSGTVYVASGNYNLSNA
                     QETLVANKPLSIIGQGIEVAEESGTVINSTRCSSDLSVEASDVTVQGVYFKQNDGDSN
                     CAQNAVLYVGPDSEDNPVVNTNILQNDIYGGASGVSVGGGATTTISNNLIHDNGRGIY
                     FELGEIGGASVSGNNIHDNTAAGIEIAADLTTNGGGVTVSSNTIGSNAGGGIIINSGI
                     TGISITGNTITDNLGGARTGIHTYSATGNSAHYNKIYSSQEDFGVTNEAEALFDASSN
                     YWGTSTEPYTGSSVGPYEADNNPSGSLFSKIQGNVKFRPFSSVSTFSATSTVTVSPDS
                     LADLFTNGGSISIASGAEDSVSSTSAATVSEEIEIEVATGVGTSSVVLPAGVEISMTD
                     TSPLNASLLEAGNIDVADISGLTEGLVAEGAFQWGIPNLGLTFSDPITLNIFVGTDLN
                     GKALTIYRSLSLTDDWVTDGIVSPGTCTVSEGICTFQTTKASYFVPTGDGTLPVITLL
                     GNASVSLTVGDSYIDAGATASDDTDGDLTSSIVTVNSVNTAVAGTYTVTYDVTDASGN
                     AATRATRTVTVSAPAAPSGGGVASGGSGTAAIGYVRTYPETVVAPKTIAEMTPAELQA
                     EIVRLTILLKSLQSKLGIAPVSYSFMKDLIWGSRSEDVMSLQKALNSDPDTVIAKLGM
                     GSAGNETTFFGLATKNAVIKFQKKHGINPTGFVGPFTRAKLNSLFK"
     gene            complement(9409..9636)
                     /locus_tag="P5056_02215"
     CDS             complement(9409..9636)
                     /locus_tag="P5056_02215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62561.1"
                     /translation="MGFRENLEKKGEEQSELKRRLEEINRKNQEATPSIENKVKPEEE
                     TAAERDLRKTIIENAEEVRKVLKEAYNKEQK"
     gene            10184..>10667
                     /locus_tag="P5056_02220"
     CDS             10184..>10667
                     /locus_tag="P5056_02220"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRY62562.1"
                     /translation="MENETRYKKLYEDSSDALMTIEPPDWKFTTGNRAALKMFRAHDL
                     REFVAADPWKLSPEKQPDGRFSNEKAKEMINTALKDGFSLFDWVHRRIDGENFPANVL
                     LTKMNMGGKSFVQATIRDVSEERRAAELIKERDLAEKMNKFMVGREVAMAELKKENEE
                     L"
BASE COUNT         3292 a         1923 c         2639 g         2803 t
ORIGIN      
        1 tgaggacgaa atcagagacc tcttgaagaa gcagggattt gatgacaagt gtcctgttat
       61 aagaggatca ggtctcaagg ctcttgaggc tacctctctc gatgatccat gggcacagaa
      121 agttcttgag cttgctaagg ctcttgatga atacattcct gatccggtaa gagagctcga
      181 taagccgttc cttatggcag ttgaggatgt tttctcaatt gaaggaagag gaacagttgt
      241 tacaggaaga atcgaaagag gaaggatcaa gctcggcgaa gaaatagaaa tcgtcggttt
      301 gaaggataca cagaaaacag ctgttactgg tatcgaaatg ttcaacaagt ctcttaacga
      361 gggtcttgcc ggagataacg caggtttgct ccttcgaggt cttaagaaag aggatgttac
      421 aagaggtcag gttctctgca agcccggatc agtaaatcca cacacagact ttgagtgcga
      481 agtttacatc ttgaagaaag aggaaggagg acgacacaca ccatttttta acggatacaa
      541 gccacagttc tatgttagaa caacagacgt tacaggagat gtaacattgc ctgccggaac
      601 agaaatggtt atgccaggtg atacggtaaa acttacagtt aagttggtag ccccggttgc
      661 tttggaagat aagggtaagt ttgctatcag agagggaggc aagacagtag gagccggtgt
      721 tgtaacaaag ataataaaat agccccggta gtagagcata gctcactaca gggcatgctg
      781 aagattagta attcaattca ttaagatgga agagaaggca actccaaaaa caaaaaccgc
      841 aaaggtcgcg aagcctagag tgaaaaaggc agttactgcc cctaaggttt caaaaccaaa
      901 ggataccgca aagaaagcca ctgctccaaa gagcgcatct tcggatgcag tcaagacgaa
      961 ggacggacag ttggtcagaa taagagttgc cgcttacgag aacaaggttt tagatcagag
     1021 cgtcaagcag attattgata ccgccatgag acaggacgcc aaggttagag gccccgttcc
     1081 gctacctacg gagataaaga aatataccgt aaacagaagc tctttcattg ataagaattc
     1141 cagagagcag tttgaaatga gaatccacag aagattgatt gatattctta atccttcttc
     1201 gaagattgtt gaggcgctta ccaatttgaa tctaccgtca ggcgtaagcg ttgacgtaaa
     1261 gatgatataa cgatagttaa tcaatacgac catgtacata ctcggaaaaa aacagaatat
     1321 gacgcaagtc tttgatgaaa acggaacatg ctccccggta acggcgcttt ctgttggacc
     1381 gatgactgtc gtacaggtaa aggattcttc cacaaaggac ggatacaacg ccatacaatt
     1441 tggttacgac gagatttctc tcgacaaatt agataaggca aaaagaggcc atcaggctaa
     1501 ggctgtcggt gcgaaggctt ccggatttag aactcttaag gagctccgat tgaaggggga
     1561 ggaaaaatca gacctgaagg ttggagatgt tgttgacacc tcagctttaa acgagggcga
     1621 caaggttacc gtatctgcga tttcaaaagg aaagggattc cagagcgcta taaagagaca
     1681 cggtttcaaa ggacaaccta gatctcacgg tcagaaacat tgcgaaagag cccccggttc
     1741 tatcggagga ggtcttagaa acaaggttcc aaaaggaatg agaatggccg gaagaatggg
     1801 aagcgacagg gtgactgtta gaggtttgac cgtagtgaag atcgacaaag aaaataatct
     1861 agtacttatt aagggagctg tccctggaag gaagggcacc cttgttgaga tatataaata
     1921 aataccatgg aagcaaaaat ctataacaac aaaggagtcg ccagcggttc tgtaaaattg
     1981 ccagaaaaaa tttttggcgt taagtggaac agtgacctgg taagccagac attgaattct
     2041 atgcgttcat cccagcgcgc gggaacagct gatgctaagg gtaggggaga cgtcagagga
     2101 ggaggtaaaa aaccttggaa acaaaaagga acaggtcggg ccagacatgg ttcaaccaga
     2161 tctcctattt gggtaggagg aggtgtcgcc cacggaccta aggccgagaa gaactatgat
     2221 agaaaggtaa ataagaaagc cagaaacaag gcactattta cgctcttgtc cgcaaagttt
     2281 aaggacggag aacttctttt catcgataat atagagacaa aagatatcaa gacaaaaaat
     2341 gccgctctta ctcttcagaa tctttcaaaa atagagggat ataaccaatt gaattataaa
     2401 aagggaaatc gcgccctcat cgtaacaccg acaaagagcg ataatactat aaagagtttc
     2461 agaaacttga atacagttgc ggtagaggag acaagaaata tggatcctct taccgtggct
     2521 aaatataaat atattattgt ttctaatccg gaggagagcg taaagatctt gtccgctaga
     2581 tcctaaacta acatgacaac aagaacagtt ttaaaattaa agaaaccgca cgttaccgaa
     2641 aaggcgacaa tgttgtctgt gaacgttaac ccggcctaca catttgaggt agaggcggac
     2701 atcaacaaga ccgaactcaa gaaaataatc aaggaggtct ttaaagttga ggcaacaaag
     2761 ataaacatca caaatttacc cgctaaaagc gtttttgtaa gaggaaagtg gggaacaaaa
     2821 tcgggaatga agaaggcggt cgtttattta aagaaaggag ataagataga ttttgcataa
     2881 accatgaaaa cctacagacc aacaacccca ggaagaagac agctacaaac gatcgagttt
     2941 ggaaagtttt tgactgcctc agagccacat aaggccctta caaaaggatt taaaagaaac
     3001 gtgggaagaa acagcgcggg aaggatcact gttagccata aaggaagcgg acacaagaaa
     3061 gtttacagaa tggttgattt tgcttacagc aagaagaaca ttcctgccaa gatagagaca
     3121 gtggaatatg atccattcag gtcatctttt atatctttgg ttgtatttgc tgacggtgac
     3181 agaaaatata tgatagttcc tcagagcatg aaggttgggg atactttcat aattagcgag
     3241 aaggcggaca tcaaaccggg aaacagaatg atgctcaaga acatcccagt tggtactttt
     3301 atatacaata tcgagtgtaa gcctgagggt ggggccagac ttgtaagatc agccggaaat
     3361 tatgccgagg ttatgtctaa cgcttccgga tacataaatg taaagatgcc ttcaagtgag
     3421 atcagaaaac ttgacgagag atgctatgct tgtgttggaa ccgtttcgaa tgaagagcac
     3481 aatcttgtaa gccttggaaa ggccggaaga acaaggtggc ttggaatcag accaacggtc
     3541 agaggtacgg ccaagaatcc ttgtgatcac ccatatggag gaggagaggg acgacaggga
     3601 agaggaacaa agaggccaaa gacgagaacc ggaaaggtca cgggaggcag aaagacaaga
     3661 aaatcaaaga agtattcaaa catatttatt gtcacaagaa gaggaaagaa aggaaagaaa
     3721 taaataacaa aataaaaaac gacccatctt gggtcgtttt ttaataccat attttatttt
     3781 ttaggaaagg agtataatta tactgtcgat taaaaaaggg ttaacaattt ataagtaaga
     3841 tgaacaaaaa gtttttgttg gcactcgccc ttctttcttt ggcgatgccg gtagcggccg
     3901 ccccttttga tcaaaataga gtaggcggcg cagatacatc ttcagccgcc ggagaagatg
     3961 ctaaaaatgt taaggctacg gcgactcccg aagctgtaaa tcaaggtcgt ggggaagaaa
     4021 ggagaagcga ggtcgccaat gctgttcaag agatgttgcg tgtagcagat agagacggcg
     4081 gcataggaga gagggtgaga gagatagcaa gagatcagca agatgagtct gttaaggcgg
     4141 aggatacgct taagttgtta aaaaataggg gacaattcag aaagtttgtt tttggttctg
     4201 attataagaa aatttccgat atagaagaac ttttggctag gcataatgcg aggttggctg
     4261 agctagaatc aatgcttcct caggttaaaa ccgatgcaga tagagcaata ttgtcggaca
     4321 aaataggtat aatgaagaac cttggcgcgg agatgcagca agaggttgac ggcgtaaaag
     4381 acgtaggttt cagtcttttt ggatggctaa acaaaatgat ttataagaaa taatccttat
     4441 atatgaaagg aaaatgtgat aaatgcgcag agaagccccc aatgtatttg gataagggta
     4501 agggcgttcc gggcacagag ccctgtaaat ggtgtgagag aatggttgcg ccaagggctt
     4561 ctaacaagcc gatgagaagc aagaagaaat agcggataaa ttgtaaaaaa ctacccccat
     4621 ggggtagttt ttgtttttga atgttagttg tataatttat aggtataaat ctcgttttaa
     4681 taatttatga ttttagaagc aaacatgaaa aataaatttt tatttttgtt gctcatctgt
     4741 gcagtcattg tgcccggatt ggtcactgca aagagtgaga atagcattca aaagatcaaa
     4801 aagccaaaga tctccgtggt taataatata aagaagataa aggctgttag gccaaggaat
     4861 attgttttta acggggtagt gaaggcggta aacgggaatg agtttactgt tgaggtcgcc
     4921 aaaaaagcaa attcaaaatt ggccaatgtt gtaaacgtgc agacaaaaac acaaaaggtt
     4981 aatctgaaat cagccaaggt taaaaaagta aacaaaaata gcgtcataaa agttggcgat
     5041 agggtgaaaa tatatggaat catgaaggat gcgaacaacg tcagcgaggc tagcgtaaac
     5101 gtcgtgaaga gcccaattaa attaaaaaag aaaaattaat ttttataaca aaaaatgaaa
     5161 aacaagtttc tgaaaattaa tttttcaaag tttaaaaact ttatctccgc atgtatatcg
     5221 attctttgtg tcgcgtcatt tgtcgcgcct aacattgttt ttgcggcaga tacttatttt
     5281 gatggcggtg atggctctcc ggaaacgcca tatcaaatat cgacttgcgc acaattacaa
     5341 gatatcggca cgacaacaga aggttttgat ccggccttga atactttaaa ttatgtttta
     5401 acggccaata ttgattgtac cggcacagaa gtagaaggta gtaattttat tccgatcgcc
     5461 tcctttagcg gcgattttaa cgggggtgga tatacaataa gcaacattac atcaacctat
     5521 aatgtgacca acagcggcgc aggtcttttt agtagcattc tttatccggc taatattcat
     5581 gacctaacga ttgccagctc tgtttttgtc ccgacaagca gcggtgttta tccggtatat
     5641 tccggagctg ttgccgcgca aatgtttgga ggaacaattg accacgtcaa tgttctgtcc
     5701 agcgtaacct taagtggagg tgagaatcct gaggtatcaa gagacggaac gggggccacc
     5761 ggtggattgg tggggnnnnn nnnnnaatta cttccgatgc ttatgacggt tcacagccga
     5821 gtattattag taatagcaca tctacagcct cttttgattt tcataacacc ataaatgcta
     5881 atggtggttt agccagttat gttggtggtt tggcgggaga catacgaact tacggtaatg
     5941 aagtaagtat atctaatagt tatgctaact ttaatttgga gaagcagtat ggtcccgata
     6001 actatggaag tgatgtctat gttatgtcta atgccggagg tttggtcggg cgcttcttgg
     6061 gcgccaatga ctccggtgat tattccggta gcttaactat ttctaactca tcagctagca
     6121 caaccattac caatgcatat aatagcgggg gacttattgg ggccgctttt ctttacgaca
     6181 atctaagtgc agaaggaggg ggcgcggaat ttaccataga ggacagcagc tcttcgggta
     6241 gtataacagc agacgctgaa actgacggga aaaacctagg aggagtgctc ggttatcagg
     6301 aggtagcagc gggatctgtt aatacgatta caaacataac aagatctcac tcatctgttt
     6361 caatttttga ctctcccgtt tccgatgacc tcgaggtcta tctgagttgc gcgggtggtt
     6421 tagtcggcga agtttatgga ctcggaagct ttaatattac tgaaagttat tctacgggca
     6481 atattggtag ctcagaagga tcccatcaac taggcggtct tgtcggagag ttatattcac
     6541 agagctccgg aggagaaggg gtgaacatta ccagaagtta ttccacaggt gatgtttatg
     6601 ggtatttgga ccttggggga attttagggg gcacaatctc agatggcgct agttttgact
     6661 tatcaattac gaattcctat tcaaccggtg atatcggtaa tggagagtcc tccacgaacg
     6721 tcggtggaat agtggggaat ggcgattact taaaactggt tattgatagc acatacgctt
     6781 caggcgatat cggccttgat atttatgcta gtgccggagg aatcgtaggc tcaatcaata
     6841 gtgattctag cgtaagcaac agtttttact cgccggcaat agttctagcg gcatattatg
     6901 gtgaaactag ttccgcgata gttggctata atggaggagc aacactcact ggaaattatt
     6961 atactaatgt gcccgatctt gaggatgacg gatatttggc gggcggagtc gattccttta
     7021 agggaaacgg tattataacg aataatctat ttgaccccgt atgggataca agcgttactc
     7081 ctatctgggt gcttactgcg ggagcatacc cgactcttaa cgcaattgcc gccgataatc
     7141 cggtagccgc accttcaagc gtttatgtta atgaaaacta taatccgtcc tatgccggcg
     7201 gactcgtctg ggaggtgaac gcttttaaca agatccagcg ggcaatgaat gcggtggcat
     7261 ctagcggaac agtttatgtg gcctctggaa attataactt gagcaatgcg caggaaacgc
     7321 ttgtcgcgaa taagccgctc agtattatcg gtcagggaat agaggtcgcc gaagaatcgg
     7381 gaactgtgat aaatagcacg aggtgttcta gtgatctctc tgttgaggcg agcgatgtta
     7441 cggtccaggg ggtttatttt aaacagaatg atggcgatag taattgtgcc caaaatgctg
     7501 ttctttatgt cggacccgac tcggaagata accctgtggt taatactaat attcttcaga
     7561 acgatattta tggaggagcg tctggcgtat ccgtgggggg aggtgcaaca acgacaataa
     7621 gcaataacct tatacatgac aacggcagag gaatttattt tgaacttggt gagatcggtg
     7681 gcgcttccgt ttccggaaat aatattcatg acaacacggc ggcaggtata gaaattgctg
     7741 cggacctaac cacgaacggg ggtggggtta ccgtttcctc caatacgata ggctctaatg
     7801 ccggcggcgg aattataata aacagcggta taacaggaat ctctataaca ggaaacacta
     7861 taacagataa tcttggtggc gcacgaacag ggattcatac atattctgcc acaggcaact
     7921 ctgctcatta caacaaaatt tatagtagcc aagaagactt tggagtcaca aacgaggccg
     7981 aggcattgtt cgatgcatcg agcaactatt ggggcacgtc tacggaacca tacacaggat
     8041 catctgtcgg tccttatgag gcggacaata atcctagcgg aagcttgttt agcaaaatac
     8101 aaggaaatgt aaaatttagg ccattctcta gtgtatctac atttagtgca acaagcacgg
     8161 taactgtaag tccggacagt ttggcggatt tgtttacgaa tggaggcagt atttctattg
     8221 cttccggagc cgaggatagc gtatcttcta catccgccgc gaccgtttcc gaagaaatag
     8281 agatagaagt ggctaccggt gtcggaacca gttcggtagt tctcccggca ggagttgaaa
     8341 tatctatgac ggacaccagt cctttaaacg cttctctttt ggaggcgggc aacatagatg
     8401 tagcggatat ttccggctta actgaggggc ttgtggctga gggagctttt cagtggggta
     8461 ttccaaattt ggggttaact tttagtgacc ctataacgct caatatcttt gtcggtacgg
     8521 atttgaatgg aaaggccctt actatttatc gatctttgag tcttactgat gattgggtaa
     8581 ctgatggtat tgtatctcct ggtacttgca ccgtaagcga aggtatctgc acattccaaa
     8641 cgaccaaggc atcatatttc gttccaaccg gcgatggaac actccctgta ataactttgt
     8701 tgggtaatgc ttctgtgtct ttgactgttg gggatagcta tatcgatgca ggcgcgacag
     8761 cttcagacga tacagatggt gacttaactt cttctattgt cacggtaaat tctgtaaata
     8821 cggctgtggc gggaacatat accgttacat atgatgttac agatgcttcc ggtaacgccg
     8881 ccacaagggc aacaagaact gtaacagtaa gcgcgcctgc ggctccaagc ggcggcggag
     8941 ttgcttcggg aggttccgga acggcggcga ttggttatgt gagaacctat ccagagacag
     9001 tagtcgctcc aaagaccatc gcagagatga ccccggcaga gcttcaggct gagattgtga
     9061 gattgacaat attattgaag agcttgcagt ctaaactcgg catagctcca gtttcatata
     9121 gctttatgaa ggacttgatt tggggttcta ggagcgaaga cgtaatgagc ttgcagaagg
     9181 ctttgaatag tgacccagat actgttatcg ctaagttggg catgggatcg gccggcaatg
     9241 aaacaacctt cttcggtctt gccacgaaga atgctgttat taaattccag aagaagcacg
     9301 gcatcaatcc gacaggtttt gtcggtccgt ttacaagagc caagttgaac tcgctattta
     9361 aatagttgaa tttaaaaacc gcccggatcc gggcggtttt tgtttgtgtt atttttgttc
     9421 tttgttataa gcctctttga gaaccttacg aacctcttca gcattctcaa ttattgtttt
     9481 ccttaaatct ctttcagccg cagtttcttc ctccggtttt actttatttt ctatagaggg
     9541 ggtcgcttcc tgattttttc tgtttatttc ttccaatctt ctttttagtt cactttgttc
     9601 ttctcctttc ttttctaaat tttctctgaa gcccataatt tattaatagc tatctgataa
     9661 gtatctatat tttgggtttt ttgtttgttt ttgtaaagga gaaggtgaaa tatatacagt
     9721 ttcaccttgt tcgtgtgtta taatttattt cgtataacaa attcatcatc ggtcccggaa
     9781 aatataatat tgaatcatgt tatagattct aaaataataa cttaatttta taaaaatgga
     9841 gacagagaaa ataaatttag agagtagggg ctcgtatttt cttttgataa tgtgtttaac
     9901 cgtcatcgct ctgttggtca gtttattatc tttgcaagcc ggttattata ttatttttca
     9961 aaatttattt tattttcccc ttatcttagc ttgcgtttat tatttttgga gaggttttgt
    10021 gttttctgta tttttggcgg caagttattt cgctttaata ttcaccttta caagtgatct
    10081 aactatttta gagggggcta taacaagggt cattatcttt atattagtcg catggttagt
    10141 tacttattta gcggtcgaga gagatagctc ggtgttaagg ttattggaaa acgagacgcg
    10201 ttacaagaaa ctttatgaag attcgtctga cgcgcttatg acgatagagc cgcctgactg
    10261 gaaattcact acagggaata gggccgcgtt aaaaatgttt cgggcgcacg atttgcgaga
    10321 atttgtcgct gcggatccgt ggaaattgtc tccggagaag caaccggacg ggcgcttttc
    10381 taacgagaag gcaaaagaaa tgataaatac cgcactgaag gacggtttta gcctgtttga
    10441 ttgggtgcat agacgaatcg acggggaaaa ctttccggct aacgttcttt tgacaaaaat
    10501 gaatatggga ggaaaatcat ttgtccaggc gaccattaga gatgttagcg aagagaggag
    10561 ggcggcggaa cttataaaag agagagattt ggccgaaaag atgaacaagt ttatggtggg
    10621 cagagaggtg gcgatggcgg aacttaagaa ggagaatgaa gagttaa
//
LOCUS       DAOTEX010000025        10507 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3118
            NODE_4448_length_10507_cov_4.354095, whole genome shotgun sequence.
ACCESSION   DAOTEX010000025 DAOTEX010000000
VERSION     DAOTEX010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868142
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 10507)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10507)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 883
            CDSs (total)                      :: 833
            Genes (coding)                    :: 829
            CDSs (with protein)               :: 829
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10507
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4448_length_10507_cov_4.354095"
                     /isolate="AMR_MDS_3118"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(257..1759)
                     /gene="infB"
                     /locus_tag="P5056_02225"
     CDS             complement(257..1759)
                     /gene="infB"
                     /locus_tag="P5056_02225"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HRY62563.1"
                     /translation="MTNKPQNKTTERPPIITVMGHIDHGKSTLLDYIRKANTVEKEAG
                     GITQHTSAYEVVHEMQSGESKRITFIDTPGHAAFSKMRGRGAKVADIIILVVAADDGV
                     NVQTIEAIKTIQDSKTPYVVAINKIDKPNSDVERTKLDLASNGVFLEGYGGDVPNVPI
                     SAKKGDGVSELLDVLLLMAEMAELKKNDGENGEGFVFESNLDSKKGGVATLIIKNGTL
                     RVGDFLIIDGNVTPVRSLENFAGKKIESAIASSPVKIIGFSKLPSVGSDFLSLDNKKE
                     AEKIAETQIPVCAKRAEENYDNAKLVLPVIIKADFQGTIEAIEKELRKLETDEVKIKI
                     ISCGVGNITENDMSLAGGKIKPLIIGFNVKADRDVIELGEKLGAKPMIFDIIYKINDT
                     IEEEIKTRMPKEEVEKTIGGARILRVFSKQKDRQVVGGTVLSGSIVVGKKVKIIRQKN
                     EIGRGEITELQENKVKTKEVAEGKQFGVTIDSKITIAEGDIIEAFDLVTI"
     gene            complement(1867..2331)
                     /locus_tag="P5056_02230"
     CDS             complement(1867..2331)
                     /locus_tag="P5056_02230"
                     /inference="COORDINATES: protein motif:HMM:NF025935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4446 family protein"
                     /protein_id="HRY62564.1"
                     /translation="MNINSPYITYALMATVAILLILAIRLEIRLRRMLRGNRVKDFEG
                     TVVSALKDIEKLAEAKLEIEKEIQNIKDKVRRNIQTAGTVRFNPFEDAGGNQSFASAF
                     LDDHGDGVVISSLYSRDKVSVFAKPIKKMKSDYELSEEEKEAMRIAWEKNKE"
     gene            2382..3578
                     /locus_tag="P5056_02235"
     CDS             2382..3578
                     /locus_tag="P5056_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012547864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HRY62565.1"
                     /translation="MAPVMKKPVKLKKRIFLDHASTTPVLSEVLSTMKKFWSSEFGNP
                     GSIHSFGLAANKALEDARKSIAMVLGARSSEIVFTSGGTESDNIAILGVAKLFKKNAN
                     GTKLHIITTEIEHHAVIHPMKFLENSGFEVTYLPVSEKGIVSVGDIEKAIKENTVLVS
                     IIYANNEVGTIQPIREIGNMIKKFRGDRPYPLFHTDACQAPGYLNLQVSNLGVDLMTI
                     SGSKIYGPKGVGLLYVKTGTPIEPIIFGGGQEAGLRSGTQPVPLIVGLARAFKMSEDK
                     KKKESERLTKIRDYFISTVVKKIEGAKLNGDAKLRLPNNANIYFPGVSGEQLVIELDV
                     RGIAASTGSACSSREEAPSHVLRAMYNDKKRADSSVRFTFGRDTVKKDIDDTIKILGS
                     IINRIR"
     gene            3627..4499
                     /locus_tag="P5056_02240"
     CDS             3627..4499
                     /locus_tag="P5056_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HRY62566.1"
                     /translation="MEELNKTQIILLAIFITFVTSVATGVVVVTLLDQAPQGITQTVN
                     RVVERTVEKVVSPGQVERTEVKQIVREDDTVVSSVQTAKQALVKVVRQGATSDVGLSN
                     PELQTASFYSTFALSEDVSRVGFVVSGDGLVISSNELLGGLGSVYFALSGDTKYNLKF
                     IKSDDKAGIALFSIENPPKNLVSLNALSIDQVSLGQTVMTLGFDSGMNTLAMGYISGL
                     KTGNASTTPVIKTSIRPTENWNGRPLIDINGFLIGMYGGDNEIIPYITIKNLIDSSSP
                     KPAAASPVSTKGAQ"
     gene            4587..7256
                     /locus_tag="P5056_02245"
     CDS             4587..7256
                     /locus_tag="P5056_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014936265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRY62567.1"
                     /translation="MIPFNNLTSKAREAIRKAHELVIERGQNQVNPIHLLVSLLIQED
                     SLILSILDKLEIDTNLLMDQLLENVEGTESPDVLSPSYQIYLTADLVRVLDGSAKVAA
                     SLKDEFISTEHLFLSMLEEQSPARDILNRFKINKEEILKVLQDIRKGKAGDAVGKKKP
                     RALEKFTRNLTKLAKEDKLDPVIGRDEEIRRIMQILTRRTKNNPVLVGEAGTGKTAVV
                     EGLAIRIAKGDVPETLKDKEIVSLDLGTLIAGTKFRGEFEERIKAILKDIEKASGSVI
                     LFIDEIHTIVGAGAAEGSLDVSNMLKPALARGELRAIGATTLNEYQKHIEKDPALARR
                     FQPVHVGEPSIEDGIAILRGLKDRYAIHHGIKITDAAIVAAVNLSSRYIADRFLPDKA
                     IDLVDEASSSLRLQLEDKPIVIQDTDKKIMRLEVEKEALKKEDSSKDSNAKARLKKID
                     KEIADLREKISEVDMRWKNEKKLIAETRAIKKELDSLRLEAEAAEMRSDLSLAAEIRY
                     GKIPTKERSLANLESKLDKLQPRRLLREEVTEEDIANVVSKWTGIPVFRMLEEEADKL
                     QRMEEELKKRVVGQDEAIRKISEAIRRSRAGVSDPDRPIGSFMFLGPSGVGKTELART
                     LSQFLFNDEKAIIRIDMSEFMEKHSVSKLIGSPPGYVGYDEAGKLTEMVKHRPYSIVL
                     FDEIEKAHPEVFNILLQVLDNGRLTDSKGRTVNFKNTVIIMTSNVGSEFVNKMETIGF
                     GNGKNEKTEYNMMKEKIMDRLKDNFRPEFLNRLDEVLIFDTLSMDVIKTIVGVQLDAV
                     KKRLTDKGVNLEVSPEVFNILATEGYNPQYGARPLKRLIQTKILNKVAEYLISKSLGP
                     KSVVVVSVKNGDIMVGIKEALSERVAGGKKVRK"
     gene            7295..7377
                     /locus_tag="P5056_02250"
     tRNA            7295..7377
                     /locus_tag="P5056_02250"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7329..7331,aa:Tyr,seq:gta)
     gene            7513..8001
                     /locus_tag="P5056_02255"
     CDS             7513..8001
                     /locus_tag="P5056_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62568.1"
                     /translation="MRKIGVLFSYNKDVIDVLSAMAEIDSERLGERQSLADVLRNVIR
                     AYIESRSGEKFILDSIIERNRVKGCQCYRCTGVIRINPLSRKSAVSFNLSVDEFEILR
                     AMAELDGQKDGPESISDQIHGAIRNYIKSRSGEMPMIDGIVRRNKEESEERLRKLLKP
                     RS"
     gene            8132..8374
                     /locus_tag="P5056_02260"
     CDS             8132..8374
                     /locus_tag="P5056_02260"
                     /inference="COORDINATES: protein motif:HMM:NF020037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRY62569.1"
                     /translation="MVESSEDAIIGKDLDGIIISWNRGAEKMYGYLADEIKGQSVKKL
                     LPGEDASEIENILKRLGGGEQFALMIQREKERMARL"
     gene            8407..8610
                     /locus_tag="P5056_02265"
     CDS             8407..8610
                     /locus_tag="P5056_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62570.1"
                     /translation="MDGEIIGASTVARDITVNKKLEAELAARAEDLEKINKFMLGREL
                     KMAELKNRIAELEAKIAGLEKSR"
     gene            8804..9565
                     /locus_tag="P5056_02270"
     CDS             8804..9565
                     /locus_tag="P5056_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62571.1"
                     /translation="MHEQLVSFIEEYLKKGSTKEEIKSVLMRVGGWTEQEVAESFAVV
                     DSKRAPAPQSLPTPPMPSKPPVQTPPSVTPPRPAMPSFQPPRPSVTGFQTSSASAQSL
                     SPRPTMPPYQSPIAQKPPIVSPAIAYTTPTIAEPMKKPPVTQVYAMPAKAQSSSGALL
                     PVLISSVISAALGVGATVAYFTYIGTVPVTIPMQIETKNVVENTTPVVPSDIQEVATS
                     TLPVLPQEELPASSTVPAPANPVSPVQPEAPFVND"
     gene            9636..10265
                     /locus_tag="P5056_02275"
     CDS             9636..10265
                     /locus_tag="P5056_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRY62572.1"
                     /translation="MEPHNGTFNFWGAGKANGAPFYLFFDTETTGLPKDWKAPVTKLD
                     NWPRLIQIAWVLYDEEGNTLEERSYIVKPDGFEIPKVASDIHGITTEMALREGRPLVD
                     VLKEFSQVADKAKRLVAHNMSYDIMIIHAELLRNGLPCGIEKKVKICTKESSTNFCAI
                     DGPYGYKWPKLNELYMKLFNEELKDAHDALVDVKATAKCFWEMKKRGVI"
     gene            10278..>10507
                     /locus_tag="P5056_02280"
     CDS             10278..>10507
                     /locus_tag="P5056_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62573.1"
                     /translation="MKPISKTDYIIYRECHKNAWYKRHRPDVYFASELSAFEKNIIET
                     GNEVDVLARGLFPDGVTVEGRGEDAVAQTAGY"
BASE COUNT         2992 a         2121 c         2432 g         2962 t
ORIGIN      
        1 attcatagag aaaatctcaa acggcaaatc aatgatcact gtcacagcga tcattgattt
       61 gccgtttgag tttttctcta tgaattccgc agcgagcttt tggaccatag aagccacctt
      121 ctcgtttctt atttcttgca tttacccggt gaaattaact ctattaaatt gcaatactct
      181 ggttttctat actaatatat tttattccaa aaatcagcta tttccgattt cgcctggggg
      241 tggggatata ttgcctttaa attgttacta agtcaaaggc ctctattatg tcgccttccg
      301 cgatagttat ttttgagtct atggtgacac caaactgctt tccttcggcg acctcctttg
      361 ttttaacctt gttttcctga agctctgtta tttctcctct gcctatctcg tttttctgtc
      421 taattatttt taccttcttg ccgaccacaa tcgaaccgga aagaacggtt ccgccgacaa
      481 cttgcctatc tttttgtttg ctgaatactc ggagtatcct tgccccccca atcgtttttt
      541 cgacttcttc ttttggcatc ctggttttta tctcttcttc gattgtatcg ttgatcttat
      601 agatgatatc aaagatcatg ggcttggcac caagtttctc cccaagctct attacatcgc
      661 ggtcagcctt tacgttgaag ccgattatta gcggttttat cttccctccg gcaaggctca
      721 tgtcgttctc ggtaatgttt ccgactccgc acgaaataat ttttattttt acctcatctg
      781 tttccagttt cctcaattct ttttcaatgg cctcgattgt gccttgaaaa tcagccttaa
      841 tgataacagg aagaactaat tttgcgttat cataattttc ctcagctctt ttggcgcaga
      901 caggtatctg cgtttctgct attttttcag cctctttctt attgtccagt gacaaaaagt
      961 cagagccgac agaaggaagc ttgctgaacc cgattatttt aacaggggac gaggctatgg
     1021 cgctttctat ttttttgccg gcaaaatttt ctaagcttct taccggagtt acattgccgt
     1081 caatgataag aaaatctccc actcgaagtg ttccgttttt tatgataagc gtagcgacgc
     1141 cacccttctt tgaatcaaga ttggattcaa aaacaaaacc ttctccgttt tcgccgtcgt
     1201 tcttttttaa ctcagccatt tctgccataa gtagtaaaac atctaggagt tcagaaaccc
     1261 cgtctccctt ttttgccgat atcggcacgt ttggaacatc tcctccatat ccctctaaga
     1321 atacgccatt tgatgcaagg tccaacttcg ttctttctac gtctgagttc ggcttgtcaa
     1381 ttttgtttat cgctacaacg tatggagttt ttgaatcctg aatagttttt atggcctcga
     1441 ttgtttgcac gtttacgccg tcatctgccg cgacaacgag gattattatg tcggcaactt
     1501 tcgcacccct tcctctcatt ttagaaaaag ccgcgtgtcc gggggtgtcg atgaatgtta
     1561 ttctttttga ttctccgctt tgcatttcgt gcacaacttc atacgcgctc gtgtgttgtg
     1621 ttattccgcc cgcttctttt tctacggtgt ttgcctttct tatataatca agaagagttg
     1681 atttgccgtg atcaatatgc cccatcactg taatgatcgg cggtctttcc gttgttttgt
     1741 tttggggctt atttgtcatt tttttacccg cttaagtttc ttaataatta tttaactggc
     1801 ttttatattt gtgtatatct gtttttattg tgccgatggc gccctgtgtg aagaaatttt
     1861 ggcgggttat tctttatttt tctcccaggc tattctcata gcctcttttt cttcttcgga
     1921 gagctcataa tctgacttca tttttttaat cggtttagca aaaacgctga ccttgtctct
     1981 ggaataaaga cttgagatta caactccgtc tccgtgatca tcaaggaagg ccgaagcgaa
     2041 actctggttg ccgcctgcgt cctcaaacgg attaaatctt accgtgcctg ccgtttggat
     2101 atttcgtcta actttatctt tgatattttg tatctctttc tctatttcaa gttttgcctc
     2161 ggctagtttt tctatgtctt ttagtgcgga aactacggtt ccttcaaagt ccttgaccct
     2221 gttgcctctt agcatccttc ttagcctaat ttcaagcctt atggccaaaa ttaataatat
     2281 ggcgacagtc gccattaacg cgtaagttat atatggtgag ttaatgttca tagtaggtaa
     2341 tattacctct ttttttgcta ttttgcaaaa aattcagtat tatggcgcct gtaatgaaaa
     2401 agccggtcaa attaaagaaa agaatatttt tggatcatgc ctctacgact ccggttctta
     2461 gtgaggtttt gtcgacgatg aaaaagtttt ggtcttccga attcggcaat ccgggctcga
     2521 tacactcttt cggtttagcg gcgaataagg cgctagaaga tgccagaaaa agcatagcta
     2581 tggttttggg ggcaaggtct tctgaaattg tttttacgag cggagggacc gaatcggata
     2641 atattgcaat tcttggcgtc gccaaattat ttaagaaaaa tgcgaatggg acaaagcttc
     2701 atatcatcac aacagaaata gagcatcacg cggttattca cccgatgaaa tttttggaaa
     2761 attcaggttt tgaggttaca tatttgccgg tatcagaaaa aggaattgtt tctgtgggcg
     2821 acatagagaa ggcaattaaa gaaaacaccg ttcttgtttc aataatatac gcaaataatg
     2881 aggtcggaac tatacagccg ataagggaga tcggaaatat gattaaaaag tttagaggcg
     2941 atagacctta tcctttgttc catacagatg cttgtcaggc gccggggtat ttaaatttgc
     3001 aggtttccaa cttgggtgta gatcttatga caatctcggg gtctaaaatt tacggtccca
     3061 agggcgttgg tcttttatat gtaaagaccg gcactccgat tgagccgata atcttcggcg
     3121 gaggacagga ggcgggattg cgttccggca cacagcccgt tccgcttatc gtcggtctgg
     3181 ctagagcgtt caaaatgtca gaggacaaaa agaagaaaga atcagagagg ctaacaaaaa
     3241 tcagagacta tttcatctca acagtagtta aaaaaataga aggggccaaa ttaaacgggg
     3301 atgctaaatt aagactacca aacaacgcca atatctattt ccccggcgtt tcgggtgagc
     3361 aattggtcat agaattagac gtcaggggga ttgcggcatc gactggaagt gcttgtagta
     3421 gcagagaaga ggccccgtcg cacgttctaa gggccatgta taatgataaa aagagggcgg
     3481 atagctcggt aaggttcact tttggaagag atactgtcaa gaaagatatt gacgatacga
     3541 taaaaatttt gggttctata ataaatagga tcagataatc gcccaaatct tccattattt
     3601 atttctgtgt tgtagaataa aattatatgg aggaactcaa caagacacaa atcattcttc
     3661 tagcgatatt tataactttt gtcacttcgg tggcgacagg cgtcgttgtt gttactcttc
     3721 ttgatcaggc cccccagggc atcacacaga cggtcaacag ggttgttgaa agaacagtag
     3781 aaaaagtcgt gtctccgggc caggtagaaa ggactgaggt taaacaaatt gttcgcgagg
     3841 atgacacggt agtctcttcg gtgcagacgg ccaagcaggc ccttgtgaaa gttgtgagac
     3901 aaggagcaac gagcgatgtc gggttgagca atcctgaatt acagacagcc tctttttata
     3961 gcacgtttgc gttaagcgag gatgtaagca gggtggggtt tgtagtctcc ggtgacggac
     4021 ttgtaatctc aagtaacgaa ttactcggcg gtctaggctc ggtttatttt gctctttcgg
     4081 gggacacaaa atataatttg aaatttatta aaagtgacga caaggccggc atcgctcttt
     4141 tctcgataga aaatcctccg aaaaatcttg tctcactaaa tgcgctttct atagatcagg
     4201 tgtcgttggg ccaaacagtt atgacgctcg gcttcgattc ggggatgaat acgctagcca
     4261 tgggttatat ttccggctta aagaccggca acgcttccac gacccccgtt ataaagacaa
     4321 gcattaggcc gaccgagaac tggaacgggc gaccgctaat agatataaac ggatttttga
     4381 ttggtatgta tggcggggat aatgagataa ttccatatat aactattaaa aacctcatag
     4441 attcgtcatc cccaaaaccg gctgccgcaa gccctgtttc gacaaaagga gcccaataat
     4501 ctatcttttc taaaattgac cccttaatca ttgacaatgg ccttttgggc tattagaata
     4561 acccaaatca acttaattta aataatatga ttccatttaa taatttaacc tcaaaggcgc
     4621 gagaagccat aagaaaggct catgaactcg taatagagag gggccaaaat caagttaatc
     4681 cgatacatct tttggtgtct cttttgatac aggaggacag tcttatcttg tctattttgg
     4741 acaagcttga gatagacaca aacttgctga tggatcagct tttggagaat gtagagggca
     4801 cggaaagtcc ggacgttctt tctccttctt accaaatata tttgactgct gatttggtga
     4861 gggttctaga cggatcggca aaggtggccg ctagcctgaa ggacgaattt atttccaccg
     4921 agcacttgtt tttgtctatg cttgaagagc agagccccgc aagagatatt cttaatagat
     4981 ttaaaataaa caaagaagaa attctcaagg ttcttcagga tataagaaaa ggaaaggcgg
     5041 gggacgccgt cggcaagaaa aaaccgcgag ctcttgagaa gttcaccaga aatcttacga
     5101 agcttgcgaa ggaagataaa cttgatcctg tgattggaag agatgaggag attagaagga
     5161 taatgcagat actcacaaga agaacaaaaa ataatcccgt tcttgtcgga gaggccggca
     5221 caggaaagac ggcggttgtg gagggccttg cgataagaat cgcgaaaggt gacgttcccg
     5281 aaactcttaa ggacaaagag atcgtttccc ttgaccttgg tactcttatc gccggaacaa
     5341 agtttagggg agagtttgag gaaaggataa aggctatttt gaaagacata gaaaaggctt
     5401 ccggttccgt tattctcttt atcgacgaga tacatacaat cgtcggcgca ggtgcggccg
     5461 aaggttctct cgatgtttca aacatgttaa agccggcgct cgcaagaggc gaactcagag
     5521 ctatcggagc gacaactctc aatgaatatc aaaaacatat tgagaaagat ccggctctcg
     5581 caagaagatt ccagcctgtg cacgtcggag agccgagcat tgaagacggc atcgctatac
     5641 ttcgaggact caaggacaga tatgctattc atcatggaat aaaaattacc gatgccgcta
     5701 ttgtggccgc ggtaaatttg agtagcagat atatcgccga cagattttta ccggataagg
     5761 ccatcgatct tgtggatgag gcctcctcgt cactccgact acagctcgag gacaagccga
     5821 tcgttatcca ggatactgat aaaaaaataa tgaggcttga agttgaaaaa gaggcgctca
     5881 aaaaagaaga tagttcgaaa gacagtaatg ctaaggcgag attaaagaag atagataaag
     5941 aaattgccga tctcagagaa aagatctccg aggttgatat gcgctggaag aacgaaaaga
     6001 aattaattgc cgagacaagg gccataaaga aggaattgga ttccttaagg cttgaggctg
     6061 aggccgcaga gatgagatca gatttgtctc ttgctgcgga aataagatat ggaaaaatac
     6121 cgacgaaaga aaggtcgctg gctaatctcg aatcaaaatt ggacaaactt cagccgagaa
     6181 ggcttttgcg agaagaagtt acagaagagg atatcgcaaa cgttgtttca aagtggacag
     6241 gtattcctgt atttagaatg cttgaagagg aggcggataa acttcagaga atggaagaag
     6301 agcttaaaaa gagggttgtc ggtcaggatg aggccataag aaagatctcg gaagctatta
     6361 ggcgctcaag ggcgggtgtt tcagatccgg acagaccaat cggttcattc atgttcctcg
     6421 gtccatcggg cgtaggaaag acagaattgg ctaggacgct gtcgcagttt ttgttcaatg
     6481 acgaaaaggc gataatcaga attgatatgt ccgaatttat ggagaaacac tctgtctcca
     6541 agcttatcgg ttcgcctccg ggatatgtcg gctatgacga agccggcaag ctgacagaga
     6601 tggttaaaca cagaccatat tccattgttc tttttgacga gatcgaaaaa gctcatccgg
     6661 aagtattcaa catacttctt caggttcttg ataacggaag acttaccgat tcaaagggga
     6721 gaactgttaa cttcaaaaac actgtcatca ttatgacctc aaacgtcggc tccgaatttg
     6781 taaacaaaat ggagacgatc ggatttggaa acggcaagaa tgagaagacc gaatacaata
     6841 tgatgaagga aaagataatg gatagattga aagataattt cagaccggaa ttcttgaaca
     6901 gactagacga ggttctcatt ttcgatactt tgagtatgga tgtgataaag acaatcgtcg
     6961 gtgttcagct agacgctgtt aagaagagac taacagacaa gggagtaaac ttagaagttt
     7021 ctcctgaggt gtttaatatt cttgctactg aaggttacaa ccctcagtat ggagcaaggc
     7081 ccctgaagag acttatccag acaaaaatat tgaacaaagt cgccgagtat ttgatatcaa
     7141 agagcctcgg tccgaagtcg gtggtggttg tcagcgtcaa aaatggtgat ataatggtcg
     7201 ggattaaaga agcgctatcc gaaagagtgg ctggcggcaa gaaagttaga aagtagttga
     7261 aaattagata gcggcggcgg aaacgtcgcc atttgcgcag gtggtagagt ggtcaattac
     7321 aacagactgt aaatctgtcg gcttcggcct acgaaggttc aaatccttcc ctgcgcacaa
     7381 aacaaccacc gaaatgtaaa aacattttgg tggttttgtt ttacggtgag taaggatttg
     7441 aaccgtgttt gtaaccattt ttatttttta ttcgttatat cgtttagaca agcacaaaaa
     7501 ggggggttca aaatgagaaa gattggggtt ctctttagtt acaacaaaga cgtgattgat
     7561 gttcttagcg caatggctga gattgatagc gaacgccttg gagaacggca atccctggcc
     7621 gacgtattgc gtaatgttat acgcgcctac attgagtctc gttccgggga gaagtttatt
     7681 ttggatagta tcatcgagag gaatagagtc aagggatgtc agtgctatcg gtgtacgggt
     7741 gtaattagaa tcaatccgtt gtcgcgaaag tccgctgtga gttttaacct ttcggtcgat
     7801 gaatttgaga ttcttcgagc gatggcagag cttgatggtc aaaaggacgg tcccgagtcc
     7861 atctctgatc agatccatgg cgctattcgg aactatatca agtcccgttc cggagagatg
     7921 ccgatgatag acggcatcgt ccggaggaac aaagaagaaa gtgaggagcg tcttcgtaag
     7981 cttttgaagc cgcgttccta gttgctgcgc cgccggacac aaccggcggc tttttatgct
     8041 aaaatgaatt agtcttaaga ataataaatt tatgggtcca atttctaaag aacaagaagc
     8101 gttatataaa tcatcttaat atcttgcggc aatagtagag tcgtctgaag acgctataat
     8161 cgggaaagat ctggacggga taattattag ctggaatcgc ggagccgaga agatgtatgg
     8221 atatcttgca gatgaaatta aaggtcaatc agtcaaaaaa ttattgcccg gagaagacgc
     8281 ttctgaaata gaaaatattt taaaaaggtt aggggggggg gaacagttcg cccttatgat
     8341 acaaagagaa aaagaaagga tggcacgatt atagatgttt ctattacggt atctccgatt
     8401 aaggatttgg atggcgaaat aatcggtgct tctacggtgg cgcgcgacat cacggtcaat
     8461 aaaaaattag aggcggaact cgccgcaagg gccgaggacc tagaaaagat taataaattt
     8521 atgttaggga gagagttgaa gatggctgaa cttaagaatc ggatagcgga gcttgaggcc
     8581 aagattgccg gtttggaaaa gagccgctaa ttaaactttt gccgttatct aacttagttt
     8641 attatctctt ttgcaaaacc cttttccgca caaaaatcgt gatacataaa ttttacgaat
     8701 tattctctat aaaaatataa tatttttcag cgacaacagg ctctgttcat aactttttca
     8761 catacgtgtg gtataatttt attgttaatt taaaaataat tttatgcacg aacaacttgt
     8821 tagtttcata gaggagtatc tgaaaaaagg ctccacgaaa gaagagatca agtctgtgct
     8881 gatgagagtc ggcggatgga cagagcaaga agtcgccgag tcttttgctg tagttgattc
     8941 aaaaagggcg cccgctccgc aatctttgcc tacaccccca atgccttcaa agcctcctgt
     9001 gcaaacacca ccttctgtta cgccgccgag gccagccatg ccttcattcc agccaccaag
     9061 gccgtctgta acaggtttcc agacctcatc tgcatcggcg cagtctttgt caccaaggcc
     9121 cacaatgcct ccttaccagt cacccatagc gcaaaagccg ccgatagttt ctcctgcgat
     9181 tgcctatact actcctacca tcgcagagcc gatgaagaag cctcctgtga cccaggttta
     9241 tgcgatgccg gccaaggctc aatcttcatc cggtgccctc ttgcccgtcc ttatttcctc
     9301 ggttatctcc gcggcgcttg gagtgggcgc gacagttgca tattttactt atattggcac
     9361 cgtgcccgta actataccaa tgcagataga gacaaagaac gttgtggaaa atacaacccc
     9421 tgttgttcca tccgacattc aagaggtggc aacctctact ttgccggtat tgcctcagga
     9481 ggagcttccc gcgtcttcta ctgttcccgc tccggcaaat cctgtttctc cggtacagcc
     9541 cgaagcacca ttcgtgaacg attaaactta ataagattac gaaacaccca atccatcctc
     9601 gggtgttttt atttgtgcta gaataaaaat cacttatgga accacacaac ggaacattca
     9661 atttttgggg ggcaggaaaa gcaaatggtg ctccttttta tctctttttt gataccgaaa
     9721 cgacaggcct gccgaaagac tggaaagctc ccgtgacaaa acttgataat tggccgcgcc
     9781 tcatccagat tgcttgggtg ctttatgacg aggaggggaa cactcttgaa gaaagatcat
     9841 atatcgtgaa gccggatggt tttgaaatac caaaagtcgc ctcagatatt cacggcatca
     9901 ctacagagat ggctttgcgg gaaggccggc cgcttgttga tgttttgaaa gaatttagcc
     9961 aagtggcaga caaggcaaaa cgtctcgtcg cgcacaatat gagctacgat attatgatta
    10021 ttcacgccga gcttttgcga aacggtcttc cgtgcggcat agaaaagaag gtgaagattt
    10081 gcacaaaaga atcgtctaca aatttttgcg ccatagacgg gccatatgga tacaagtggc
    10141 cgaagctgaa cgagctttat atgaaattgt tcaatgagga gctaaaggac gctcacgatg
    10201 cgctagtgga tgtgaaggcg accgccaaat gcttctggga gatgaaaaaa cgcggggtca
    10261 tttaagtaaa aattattatg aaaccaatat ctaaaaccga ttacataatt taccgcgagt
    10321 gtcacaagaa tgcctggtat aagaggcata ggcctgatgt ttattttgca tcagagcttt
    10381 cagcatttga aaaaaatata attgagacgg ggaacgaagt ggacgtgctt gcgaggggtt
    10441 tgttcccgga cggtgtaacg gtagaggggc gaggggagga tgctgtggcg cagaccgctg
    10501 gatatat
//
LOCUS       DAOTEX010000026        10207 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3118
            NODE_4621_length_10207_cov_4.170508, whole genome shotgun sequence.
ACCESSION   DAOTEX010000026 DAOTEX010000000
VERSION     DAOTEX010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868142
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 10207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 883
            CDSs (total)                      :: 833
            Genes (coding)                    :: 829
            CDSs (with protein)               :: 829
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10207
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4621_length_10207_cov_4.170508"
                     /isolate="AMR_MDS_3118"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="P5056_02285"
     CDS             <1..436
                     /locus_tag="P5056_02285"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HRY62574.1"
                     /translation="IMNQSDIVASASTITTTLTYLLSAIAGISLVVGGIGIMNMMLTT
                     VTERTREIGLRKAIGAKRGDITFQFLMEAVMLTFFGGAVGVVCGWVAAFVISYLGLID
                     ASVTWTSVLLAFSVSAGIGIIFGYYPAYRAASLNPIEALRYE"
     gene            714..1391
                     /locus_tag="P5056_02290"
     CDS             714..1391
                     /locus_tag="P5056_02290"
                     /inference="COORDINATES: protein motif:HMM:NF024956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4131 domain-containing protein"
                     /protein_id="HRY62575.1"
                     /translation="MSRELVFFGFILGVFVRSFVDLGYSFAFLFAFCGALLFFAEYFY
                     KNYLNKEVRLKEVAFIFIFFALGIGWYGLRAGDTPRVSSLRPGAQSLEVFVGEEPEEK
                     NGKLNAVVTYNEEEKILIKSSLLPSYKYGDTLRIFGDLKKPENFNDSFDYISYLGKDD
                     IYFLIDDARVKVLETGGWVNAKENTFRCARYFHTEPSRDTSRASFVSCRRSYLGGKRF
                     AWKRMAR"
     gene            1351..2190
                     /locus_tag="P5056_02295"
     CDS             1351..2190
                     /locus_tag="P5056_02295"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HRY62576.1"
                     /translation="MGAKDSLGKEWLDKFKIAGLSHIIVLSGYNLTIIADSVMKVVSR
                     FSTGILPSVFGAISVVLFTIMTGAGASTVRAAIMALIILLAKTTGRVYEASSALIVAG
                     VLMIIENPRVLVFDMSFQLSFLATLGLLYVSPLVKERLKFVTEKLGLREMLSATIGAQ
                     IATSPLIIYKMGTFSLVALPANILVVLLIPITMFFGFLAAIVGFINYYIAIPFAAVAW
                     LLLSYILSVVDIFSSLPFATINITALSVLSISVLIFACVFVYKRRDIFSSEIDQWKIE
                     EIN"
     gene            2219..3076
                     /locus_tag="P5056_02300"
     CDS             2219..3076
                     /locus_tag="P5056_02300"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRY62577.1"
                     /translation="MFKKEKFKIIILSLIVLACAFVWYAVYREDRHGVLTVSFLDVGQ
                     GDAIFIDAPNGNQVLIDAGPDKKVLRSLSEVMPFYDHEIDLIALSHPHTDHIAGFLDI
                     LPRYLVSGFIESGVSAKTPEYISLKKLTGENNIKEIEALRGMKIDLGGGATMDVLLPG
                     QNSSKDNPHEGMMVLRLVYGDTSFLLTGDLENATEDMLVYLEGENIKSDVLKVGHHGS
                     KNATHPMLLGNVKPEYAVISVGVKNMYGHPATETLERIEKFEIPVLRTDEEGTITIKS
                     DGEKIIVLP"
     gene            complement(3146..3346)
                     /locus_tag="P5056_02305"
     CDS             complement(3146..3346)
                     /locus_tag="P5056_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HRY62578.1"
                     /translation="MAKTCTICKRGSIMAGGYSNRVRATKFNPTGIVRKQVNLQWATL
                     KDGSRMKVCTRCIKGKKHLEVK"
     gene            3545..4156
                     /locus_tag="P5056_02310"
     CDS             3545..4156
                     /locus_tag="P5056_02310"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HRY62579.1"
                     /translation="MESISPIFGIAILIMSVVAHEVSHGYVAYILGDPTAKLSGRLTM
                     NPLKHIDPIGSVIVPIITSIMGFTFGWAKPVPYNPYNLRGGDKAAAAVAMAGPVMNLF
                     IAFVFGAVIKTRLLDGIMPIEIYGVLLYIVLINFVLAIFNLTPIPPFDGSKVFFSFLP
                     YSQKKIEDFLENNQLIILFIFIFFVWGSIVEPIINILIKYLLS"
     gene            4245..4661
                     /gene="rpsL"
                     /locus_tag="P5056_02315"
     CDS             4245..4661
                     /gene="rpsL"
                     /locus_tag="P5056_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014262276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HRY62580.1"
                     /translation="MSTINQLVKRPRKKVSVRTRTVALAKGFNVKKNRPVVYPSPFKR
                     GVCTVVKTTTPKKPNSALRKIARVRLTNGMEVTAYIPGIGHNLQEHSVVVIRGGKVKD
                     LPGVRYHIVRGMLDCAGVDTRRQGRSKYGMKKPKAK"
     gene            4669..5139
                     /gene="rpsG"
                     /locus_tag="P5056_02320"
     CDS             4669..5139
                     /gene="rpsG"
                     /locus_tag="P5056_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011943782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HRY62581.1"
                     /translation="MRRKIKARKPIQPDTVYKSVDLAKFINYIMIGGKKNTARTVVYD
                     ALKEVGEKAKTDKPIEVFESALNNVGPVTEVRSRRIGGATYQVPREVKPNRRMALAMR
                     WIILAAEGKKGMPMASRLADEIILASKNEGEAFKKKENTHKMAEANRAFAHFAW"
     gene            5269..7413
                     /gene="fusA"
                     /locus_tag="P5056_02325"
     CDS             5269..7413
                     /gene="fusA"
                     /locus_tag="P5056_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011393940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HRY62582.1"
                     /translation="MERLSPIEKVRNFGIIAHIDAGKTTTSERVLYYTGKKHKIGEVH
                     EGETTTDWMEQERERGITITSAAITAFWSPSYMPEGDTSKRCQFNIIDTPGHVDFTAE
                     VERSLKVLDGGVVVFDGVAGVEPQSETVWRQADKYKVPRICFANKMDRIGASFDHCYK
                     TILERLNKNCVRMQLPIGTEENHEGVIDLLRMKAYYFEGDMGNKIVEKDIPENLLADA
                     KAARADTLERIVEHDDKLMNEYLEGKEINLDELKKVIRREVIANKLFPLFCGSALKNK
                     GVQLVLDAVIDFLPAPVDIPPVKGFDPATEAELERKASDAEPLAAMAFKLQSDPFVGQ
                     LTYVRVYSGVLSSGSYVYNSTKGTKERVGRILRMHANDREEIQQVFAGEIAAVVGLKD
                     AKTSDTLCDEDKPIILNKIVFPNPVVELKIEPKTKADQEKMSFALKRLSDEDPTFKVK
                     TDEETLETIIAGMGELHLEILVDRMKREFGVNANTGKPQVAYRETVTTEAEAENKYIK
                     QSGGKGQYGHCVLTVRPLKPLEEGAKIPKNVKRDDHFEFINSIKGGVIPQEFIPAVEK
                     GVREALGKGIVAGFPIVDVSVELTFGSSHDVDSSEMAFKLAGSMAFQDACKRARPVIL
                     EPIMKVEVTTPEKFMGDVTGNLSGRRGQIEGMEDRYGVKAIKAKVPLSEMFGYVTTLR
                     SMTEGRASFVMEFDRYEIVPKNVEAAIVAGKK"
     gene            7578..8141
                     /locus_tag="P5056_02330"
     CDS             7578..8141
                     /locus_tag="P5056_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62583.1"
                     /translation="MIIGGSIHPDEFSKDPITQRHLRNMIEGERQREEDDFGPDSRTL
                     IRLTSWLNYDRDLAIVYALEKSAGTRIYFFGKCRFIVRRNMLPVLRRCEVKFEELYYN
                     GEVVCPGRKCPPPHFESGSGFLGIDPWCREYVEPWEVFVDHVIGSNAYLELMEQAIEG
                     SGVEKEQVLNSLWSRVKRFFRNYFSRE"
     gene            complement(8185..8877)
                     /locus_tag="P5056_02335"
     CDS             complement(8185..8877)
                     /locus_tag="P5056_02335"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HRY62584.1"
                     /translation="MRLHRFYIKTDLSGDKITIVDKETINQIKNVFRLRKGDQFMIFN
                     DKKEEVVATIKNISSDELSAEVSKKIKNDSEPQNKVNLYLSILKKENFELVVQKATEV
                     GVSKIIPVISARTVKMNLNIERLQKIAKEAAEQCGRQNIPEISEPIKIREAFEEAAKN
                     GVTLFFDRYGEDISTWKPGFQVNIFIGAEGGWDEWEVELAKEKGFHIASLGKLTLRGE
                     TAAIIATYLAVK"
     gene            9015..9887
                     /locus_tag="P5056_02340"
     CDS             9015..9887
                     /locus_tag="P5056_02340"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HRY62585.1"
                     /translation="MNNEKQIKRVSFFFYKDNEKAVAWEKKIRSWFEDKYPEVKIVPL
                     PKDLNNCGTSPELVIALGGDGTIIEMARKANGCGVLLMGLNLGNIGFMASVRRSEDFL
                     GGLKKVMDGKYRIDSRMKMAAEVIRGGKVISELSAVNEIVVQNPLGMVELAVDIEKYP
                     LQYIRGSGVMVATASGSTAYNLSAHGPIVMPELSCFIITELFDHNIPTPSVIIKEDKE
                     ATIKVENFRENKKMTLTDSGESADVVIVADGADIFSLKKGDTIKIRKSDDSVNFVEVE
                     PNYFFKSLKEKFAF"
     gene            10014..>10207
                     /locus_tag="P5056_02345"
     CDS             10014..>10207
                     /locus_tag="P5056_02345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62586.1"
                     /translation="MGHCVHDFSAHTPATEEELRKNERETDEIQSTAKVASLWMVVLP
                     NDCKSAEEVIYQKGFTLRPP"
BASE COUNT         2913 a         1926 c         2451 g         2917 t
ORIGIN      
        1 catcatgaat caatctgata ttgtggcaag cgcctccacg ataaccacga cactcactta
       61 tttattgtcg gctatcgcgg gaatctccct tgtcgtcgga ggtatcggca ttatgaacat
      121 gatgcttaca acggttaccg agaggactag agagataggg ctcagaaaag ccatcggtgc
      181 caaaagagga gatataacgt ttcaattttt gatggaagcg gtgatgctaa cttttttcgg
      241 cggcgcggtc ggagttgtgt gcggttgggt ggccgctttt gttatctcgt atcttggatt
      301 aatagacgct tccgtaacat ggacgtcagt gttgcttgca ttttctgtgt cggcgggcat
      361 tgggattatt ttcgggtatt atcctgcgta tagagcggcg agtttgaatc cgatagaggc
      421 gctgagatac gaatgatgaa ttaacaatta cgaattacga attttgtcat tgcgagtgta
      481 gtgaagcaat ctaggtctgg tccaaatatt tctggattgc ctgcctgcgc aggcaggctt
      541 cgcggtgctc gcaatgacgc aagtttttag cttggctaag catgacttat ggcgtaaaaa
      601 tatgccattg ccttaacaaa agtgagggct ctttttattt tttaaatatt tccacagact
      661 tttagattta tacaagttgc gagtggcgat ttgcgagagt agtatttggg ccaatgtcac
      721 gcgagctagt atttttcggt tttattcttg gtgtttttgt aaggtcattc gttgacctag
      781 gctattcgtt tgccttcctt tttgcttttt gcggagcgct tttatttttt gccgaatatt
      841 tttataaaaa ttatttaaat aaagaggtgc gattaaaaga ggtcgcattt atttttattt
      901 tcttcgctct cggaatcggt tggtacggtt taagagcggg agatactcca agagtttcat
      961 cacttcgacc cggggcgcaa agcctcgaag tattcgttgg cgaagagccg gaagagaaga
     1021 acggaaaatt gaacgcggtt gtgacgtata acgaggagga aaaaattctt ataaaaagtt
     1081 ctcttttgcc gagctataaa tacggcgata ctttgcggat attcggtgat ctcaaaaagc
     1141 cggaaaattt caacgactcg tttgactata tttcttatct cgggaaggat gatatttatt
     1201 ttttaataga tgacgcacgg gttaaggtcc ttgagacagg ggggtgggtc aacgctaaag
     1261 aaaacacttt tcgctgtgcg cgatactttc acacagagcc ttcacgagac acttcccgag
     1321 cctcattcgt ctcttgccgc aggtcttatc ttgggggcaa aagattcgct tggaaaagaa
     1381 tggctcgata aatttaagat agccggtctg tcgcacatca ttgttctttc cggctacaac
     1441 ctgacgatta tcgctgattc ggtgatgaaa gttgtttccc gattttcgac gggcattttg
     1501 ccgtcggtct ttggcgcgat atcggtagtg ctttttacaa ttatgacggg cgcgggagct
     1561 tcgactgttc gcgccgcgat aatggcactt ataatacttc ttgcaaaaac gaccggtcga
     1621 gtttatgaag cgtcttcggc gctcattgtc gcgggcgtat tgatgataat agagaatccc
     1681 agagtgcttg tatttgatat gtcgtttcaa ctgtcttttc ttgccacact tggtcttttg
     1741 tatgtttcac ctttagttaa agaaaggcta aaatttgtta cagaaaaatt ggggcttcgt
     1801 gaaatgctct ctgcgacaat tggcgcgcag atagcgacct caccgctcat tatttataaa
     1861 atgggaacat tctcgctcgt tgcactaccc gcgaatatcc tcgtagtgtt actgataccg
     1921 ataacaatgt tttttggttt cttggcggct attgttggtt tcataaatta ttatattgca
     1981 atcccttttg ccgcggttgc atggctactt ctttcatata ttttgtcagt ggtcgatatt
     2041 ttttcttcgc ttccgttcgc gactattaat attactgcgc tttctgtatt atcgatctca
     2101 gtattaatat ttgcgtgcgt tttcgtgtat aaaagaaggg atatattttc atctgaaata
     2161 gaccaatgga aaatagaaga gataaattag ttataaagtt gaaagttata aagttttaat
     2221 gttcaaaaaa gaaaagttta aaataataat actttcactc attgtgctcg catgcgcttt
     2281 tgtttggtat gcagtttata gagaagatag gcacggtgta ctcaccgttt catttcttga
     2341 tgttggacag ggtgatgcga tattcataga tgcaccgaat ggtaatcagg ttcttattga
     2401 tgccgggccg gacaaaaaag ttcttcgaag tttgagcgag gtcatgcctt tttatgatca
     2461 cgaaatagac cttatcgctc tctcgcaccc tcacaccgac catatcgccg gtttcctgga
     2521 tattttgccg cgttatttgg tttccgggtt tatcgagtct ggagtttcag cgaagactcc
     2581 ggaatatatt tcattaaaaa aattaaccgg cgagaataat ataaaagaga tcgaagctct
     2641 ccgcggcatg aaaatagacc ttggcggagg tgcgacgatg gatgttttgc tccccggtca
     2701 aaattcatcc aaggataatc ctcacgaggg gatgatggtg ttgcgattgg tttatggaga
     2761 tacttctttt ttgctgacag gggatctgga aaatgccaca gaggacatgc ttgtttactt
     2821 agaaggggag aacataaaaa gcgatgtgtt gaaagtggga caccacgggt caaaaaacgc
     2881 gacacacccg atgcttctcg ggaatgttaa gccggaatac gcggttatct ccgtcggtgt
     2941 gaaaaatatg tatggtcacc ccgctacgga gacgcttgaa agaattgaga aatttgagat
     3001 acctgtttta cggacggatg aggaggggac gataacgata aagagtgatg gagagaaaat
     3061 tattgttctg ccgtaatgag atgatattta attaaaataa aaaccgcccg gttttgctcc
     3121 gggtggtttc aaatgtaact attttttact ttacttcgag gtgtttcttt cctttgatac
     3181 atctggtgca gaccttcatt ctgcttccgt cttttagggt agcccactga agatttacct
     3241 gctttctgac gatacctgta gggttgaatt tggttgctct aactctattg gagtatcctc
     3301 cagccattat gcttcctctt ttgcaaattg tgcatgtctt agccatttac cctgttaagt
     3361 aaaaatttgt ttaattgttt ttacctattt attaccaata ttaattaatc gactgtgaac
     3421 cctgttgact gcgctgcaaa caaattttaa tttttaccta aaaatttact aaacagggtg
     3481 aatttgtttt tgaattaagt ttttttatgc tagcataaga ttaattaatt agcaagatac
     3541 gaaaatggaa tcaattagcc ctatatttgg aatagcaatt cttattatgt ctgttgtggc
     3601 gcatgaggtt tctcatggct atgtggcata tatattgggg gaccctaccg ctaaactttc
     3661 cggcaggctc acgatgaatc cattgaaaca tatagacccc atcggatctg ttattgtgcc
     3721 tatcataaca tcaattatgg ggtttacttt tggttgggct aagcctgttc catacaaccc
     3781 ctataatttg aggggaggag acaaagccgc agcagcagtg gctatggccg gacccgtgat
     3841 gaatttattt atagctttcg tgtttggcgc agtgataaaa acgagacttc ttgacggtat
     3901 tatgcccata gaaatatatg gggtcctcct gtatatagtt cttataaatt ttgtgctcgc
     3961 catcttcaac ctaacaccca ttcctccgtt tgacgggtca aaagtgtttt tctctttttt
     4021 gccttatagc cagaagaaga tagaagattt tcttgagaac aatcagctga tcatcctctt
     4081 tatttttatt ttctttgttt ggggatctat agtagagccg attataaata tactcattaa
     4141 gtacctatta tcttaagttt tgttgcaggc attttgaggc gagaccctgt tccccctagg
     4201 ccccccttga tttattttac agacctgcta tattatcgcc tacgatgtca acaataaacc
     4261 aattagtaaa aagacctagg aaaaaggtca gcgtgagaac aagaacagtg gctttggcca
     4321 agggttttaa tgttaaaaag aacagacccg ttgtttaccc gtctccattc aaaagaggag
     4381 tttgcacggt tgtaaaaaca actacaccaa agaaacctaa ctcagctctc agaaagattg
     4441 cgagagtaag gctcacaaac ggtatggagg ttacggcata tattccggga atcggacaca
     4501 atttgcagga gcactccgtt gtcgttatca gaggaggaaa ggttaaggat cttccgggag
     4561 tacgatatca catcgtaaga ggaatgcttg attgcgccgg tgtcgacaca agaagacagg
     4621 gcagaagcaa gtatggaatg aagaagccaa aggctaaata actaaacaat gagaagaaaa
     4681 ataaaagcaa gaaaacctat acagccggat acagtctaca agtcagtaga tctagctaag
     4741 tttataaatt acataatgat tggcggaaag aagaataccg cgaggacggt cgtttatgat
     4801 gctcttaagg aagtagggga gaaagcaaaa acagacaagc ctatagaggt ttttgaaagc
     4861 gccctgaaca acgtcggtcc tgtcacagag gtaagatcca gaagaatcgg aggtgctaca
     4921 tatcaggttc ctagagaagt taagccaaac agaagaatgg ctctagccat gagatggata
     4981 attttggccg ctgagggcaa aaaaggtatg cctatggctt caagacttgc ggatgagata
     5041 atactggcca gcaagaacga aggagaagct ttcaaaaaga aagaaaatac acacaagatg
     5101 gccgaggcca accgagcgtt cgcacacttt gcttggtagc tcaaaaattt tcacagacag
     5161 ctgtgaaaat tttaaattat caattaccaa ttacgaatta atttccggta attgaaaccg
     5221 aagttttttg tatttaaatt tataatttat aatttgtaat taaacattat ggaacgtctc
     5281 tcgcctatag aaaaagtaag aaatttcgga atcatcgcgc acatagatgc cggaaaaaca
     5341 acaacttccg aaagagtttt gtattatacc ggaaagaaac acaagatagg tgaagttcac
     5401 gagggagaga ctactacaga ctggatggaa caggaaagag aaagaggtat tactatcacc
     5461 tcggccgcta ttacggcttt ctggagccct tcatacatgc cggaggggga tacatcaaag
     5521 aggtgccaat ttaacattat tgatactccg ggccacgtag acttcacggc agaggttgaa
     5581 agatccctaa aggttctcga cggaggagtt gtcgtatttg acggagtcgc aggagtagag
     5641 cctcagtcgg agacagtttg gaggcaggct gataaatata aagttccaag aatctgtttt
     5701 gctaataaaa tggacagaat cggcgcatct ttcgaccact gctataaaac aattttggaa
     5761 agattgaaca agaactgcgt tagaatgcag ctccctattg gaacagagga aaatcacgaa
     5821 ggagttatcg accttctcag aatgaaggca tattacttcg agggagatat gggaaacaag
     5881 attgttgaga aggacatccc tgaaaatttg cttgctgatg caaaggccgc aagagcggat
     5941 actcttgaga gaattgtcga gcatgatgac aagctgatga atgaatatct tgagggtaag
     6001 gaaattaatc ttgatgaatt aaagaaagtt attagaagag aggttatcgc caacaagctt
     6061 ttccctcttt tctgcggatc cgctcttaag aacaagggag ttcaattagt tttggatgct
     6121 gttatagact tcttgcctgc tccggttgat attcctcctg taaaaggatt cgatcccgcc
     6181 acagaggccg agcttgaaag aaaggcttcc gatgccgaac ctttggccgc tatggcattt
     6241 aaacttcagt cagatccatt cgtgggacaa cttacatacg ttagagttta ctcaggagtc
     6301 ctttcgtccg gtagctatgt ctacaattcc acaaagggaa caaaagaaag agtaggaaga
     6361 atccttagaa tgcatgctaa cgatagagag gaaatacagc aggtcttcgc aggagaaatt
     6421 gcggcggttg taggattaaa ggacgctaaa acatccgata ctctttgtga tgaagacaag
     6481 ccgattattt tgaataagat agtattccca aatcctgttg tggaattaaa gattgagccg
     6541 aagacaaagg ccgaccagga gaagatgtcg tttgccttga agaggctttc tgacgaagat
     6601 ccgacattta aggtaaagac agatgaagaa acattggaaa caattatcgc aggaatggga
     6661 gagctccacc tcgagatctt ggtagacaga atgaagagag agtttggagt aaacgcaaat
     6721 acaggcaagc cgcaggttgc atatagagag accgtcacaa cagaagctga agctgaaaac
     6781 aaatatatca agcagtccgg aggtaaggga caatacggtc actgcgtgct caccgtcaga
     6841 cctcttaagc cgcttgaaga gggtgcaaaa attccaaaga acgttaaaag agatgaccat
     6901 tttgagttca tcaactcgat taaaggaggt gttatccctc aggaattcat tccggctgtc
     6961 gaaaaaggtg ttagggaagc tctcggaaaa ggtattgttg caggattccc tattgttgac
     7021 gtgtcggtag agcttacatt cggttcttca cacgatgttg actcttcaga aatggcgttc
     7081 aaactcgcag gttctatggc cttccaggat gcttgcaaga gagctagacc ggttatactt
     7141 gagccgataa tgaaagttga ggttacgact ccggagaagt tcatgggtga tgtcaccgga
     7201 aacctaagcg gaagaagagg tcagatcgaa ggaatggagg acagatacgg agttaaggct
     7261 atcaaggcca aggttccatt gtctgaaatg ttcggttacg ttacaacctt gagatctatg
     7321 acagaaggac gagccagctt cgtaatggaa tttgatagat acgagatcgt tccaaagaac
     7381 gttgaggctg ccatcgttgc aggaaaaaag tagttataaa atcgctaaat cgtcattgcg
     7441 agcgtagcga agcaatccag aagttaaata tattagaaac cacttggaca tgtcatgccc
     7501 aagtggtttt gtgtttgcgt ggcccgtgtg ctagattggt ttcggaataa atcgaacatt
     7561 gaaaggggag atggcgtgtg atcatcggag gaagtattca tccggacgag ttctcgaagg
     7621 atccaattac ccaaagacat cttcgaaaca tgatcgaggg cgagcgtcag cgtgaagagg
     7681 atgatttcgg tccggatagt cggacgctca ttcgattaac ttcatggctc aattacgatc
     7741 gtgacttggc gattgtctat gcgcttgaaa aaagcgcagg tactcgtata tattttttcg
     7801 gaaaatgtcg tttcatcgta agaagaaata tgctgccggt actgcgtcga tgcgaagtta
     7861 agttcgagga gctctattac aacggagaag tcgtttgtcc cggaaggaaa tgtcctccgc
     7921 cccactttga gagcggtagc ggctttctcg gaatagatcc ttggtgccgg gaatatgtcg
     7981 aaccatggga agtcttcgtc gaccatgtta tcggcagtaa tgcgtacctc gaactcatgg
     8041 aacaggctat cgaaggaagc ggagtagaaa aagagcaagt tttgaacagc ctttggtcaa
     8101 gagtaaagag gttcttcagg aactacttta gccgcgaatg agttaggacg tcgattcagc
     8161 cgagcattgt gctcggcttt ttaattattt taccgccaaa tatgtcgcga ttatcgccgc
     8221 cgtttcgcca cggagggtca gtttaccgag ggaggcgata tggaatcctt tttcttttgc
     8281 cagttcaact tcccattcgt cccatccgcc ttcggcgcct ataaatatat tcacttggaa
     8341 accgggtttc caagtggaga tgtcttcgcc gtatcggtcg aagaatagcg taacgccgtt
     8401 tttggcggcc tcctcaaaag cctctcgtat tttaatcggc tcgcttattt ccggaatatt
     8461 ttgtcttccg cattgctcgg ccgcttcttt ggcgatcttc tgaagcctct caatattcaa
     8521 attcattttg accgtgcggg cggatatgac aggaattatt tttgatactc cgacctcagt
     8581 cgctttttga acaacgagct cgaagttttc ttttttaagg atagaaaggt agagatttac
     8641 tttgttttgt ggctcgctat cattttttat tttctttgac acctcggccg agagctcatc
     8701 gcttgagata ttttttatgg tcgccacgac ctcctctttt ttatcattaa agatcataaa
     8761 ttggtctcct tttcttagtc ggaagacgtt tttaatctga tttatcgttt ctttatcgac
     8821 gatggttatt ttgtcgccgg aaaggtctgt ttttatataa aatctgtgaa gtctcatttg
     8881 tgtattttat attaaaaaaa actagattgc cacgcctcag ctaagacccg aagtagtaga
     8941 gcagagctca ctactgggca ggcgcttcgg ctcgcaatga cggaaagaag gtttcatgct
     9001 agattatcag cgtggtgaat aacgaaaaac aaattaaaag agtttccttt ttcttctata
     9061 aagataacga aaaggcggtc gcctgggaga aaaagataag gagctggttt gaggacaaat
     9121 atcctgaagt taaaatcgtt cctctgccaa aagatttgaa caattgcggc acgtcgccgg
     9181 aacttgttat cgcacttggc ggggatggga caataatcga gatggcgcga aaggcgaatg
     9241 gttgcggggt gcttcttatg gggcttaacc tcgggaatat cgggtttatg gcatctgtga
     9301 ggaggtcaga agatttcttg gggggactca agaaggttat ggacggtaaa taccgcatag
     9361 actccaggat gaagatggcc gcggaagtta ttcgcggcgg caaggtcatt tcagagttat
     9421 ctgcagtaaa tgaaattgtt gttcaaaatc ctcttggcat ggtggagcta gcggtggata
     9481 tcgaaaaata tccgcttcaa tatataagag gtagcggggt tatggttgcg acagccagcg
     9541 gctcgaccgc ctataatctt tccgctcacg gtccgatagt tatgcccgag ttaagctgct
     9601 ttattattac agaacttttt gaccacaaca ttcctacacc gagcgtgata ataaaagaag
     9661 ataaagaagc gaccatcaag gttgaaaatt tcagagagaa taaaaaaatg acgcttactg
     9721 atagcgggga gtctgctgat gtcgtcatcg tagccgatgg ggccgacatt ttctcgctta
     9781 agaaggggga cacaataaag ataagaaagt cggatgattc ggtaaacttt gtcgaagtcg
     9841 aaccaaacta ttttttcaaa agtcttaaag aaaaattcgc tttttagcag accctggaca
     9901 tatgtagaaa aatacgtagt atctctattt aagctacatt tgctctttgt aacggggaaa
     9961 ataacaataa cgtccttatg ggtctttgac caagaaaggg gagttggtga cttatgggcc
    10021 actgtgttca tgatttcagc gcgcatacgc cagctacgga ggaagagtta aggaaaaacg
    10081 agcgagagac agatgagatc cagagcacgg ccaaggtggc tagtttgtgg atggttgttc
    10141 tgcccaatga ctgcaaaagt gccgaagagg ttatttatca gaagggattc acgctgaggc
    10201 ctccgat
//
LOCUS       DAOTEX010000027        10171 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3118
            NODE_4644_length_10171_cov_4.277778, whole genome shotgun sequence.
ACCESSION   DAOTEX010000027 DAOTEX010000000
VERSION     DAOTEX010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868142
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 10171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 883
            CDSs (total)                      :: 833
            Genes (coding)                    :: 829
            CDSs (with protein)               :: 829
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10171
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4644_length_10171_cov_4.277778"
                     /isolate="AMR_MDS_3118"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(116..391)
                     /locus_tag="P5056_02350"
     CDS             complement(116..391)
                     /locus_tag="P5056_02350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62587.1"
                     /translation="MGKKKKPLFTVRPIEKELLDLLKKNGGEMKLDDIKLIKPHEKPI
                     VDMGDRGFMDIDEYNRYFILNFIEWFSEIFSYDEKTDTVRLLKRDKK"
     gene            complement(545..2359)
                     /locus_tag="P5056_02355"
     CDS             complement(545..2359)
                     /locus_tag="P5056_02355"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY62588.1"
                     /translation="MKKIFSKIRESYELLVLNAWKMDKVMWSDAKVYLVALFFFRIFT
                     STEPFLASGALALLLNELAVVASGHIVLNSFYLALGLLVASIVIPALMSSLDSFCEVM
                     LYWIAERKINLLIIKKSSEIDISLHEDPKFKDLFNRIAESGAWSSAEFVFRQFLMLDS
                     VGMVIIASAVLFWANWKIFLLVFFATIPELVNGIYYSTRVWGISDTKGEVRRRYWDIR
                     DLFLSQSSLVELNIFQSAGFFYQMMKKMFDDFKVAEKKNEIRNAIFQFFAVGLSQVAL
                     AIAIVSYAMQTASGIIGIGTLTFVLYSVQSLKRELSRCFWLFGRQYKDGLFVRDLLTF
                     WSIKTVIKKPEKGIVLDKKVAPEVVFENVSFTYPGTDKLVLKNFSFVIKPGEKIALVG
                     LNGAGKTTFVKLLCRFYDPTEGRILINGHDLREIDLDSWYHMLGAVFQDYSRYHLTVE
                     EAIAVGRTGDKPNIEKVKDAAKASEADIFIEKWDKAYSQTLGRAFTEGVEPSVGQWQK
                     LALARVFYRDPKVMILDEPTSSIDALSEANIFEKIGDVSDNRSVILISHRFSTVRKAH
                     KIVVIKDGEKAEDGTHEELMAKKGVYAELYQLQAKSYE"
     gene            complement(2455..3312)
                     /locus_tag="P5056_02360"
     CDS             complement(2455..3312)
                     /locus_tag="P5056_02360"
                     /inference="COORDINATES: protein motif:HMM:NF014510.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase"
                     /protein_id="HRY62589.1"
                     /translation="MLTIFLEILDILTTAFIIYTALVLLKKTRSIYILRGFFGLGLFY
                     IAAVFLNLKLTTSLFQAFISFFIIILVILFQKEIRRFFENFTIDFFVSAFAEEASVSD
                     NVIEIICNVVAELSKTRRGALIVLPGKQSLERVLEGGTPLNGKISEPLLLSIFDPKTP
                     GHDGAVTIEGDIVKRFMVHLPLADMNKKYPGIGTRHRAGLGLAERTDAMIIIVSEERG
                     SITIARNNDLHIVANAEKLKEEIHKFKGDGKAVKKHNKFLSFLLSDTRDKLFILAIST
                     FLWYLIVHK"
     gene            complement(3432..4667)
                     /locus_tag="P5056_02365"
     CDS             complement(3432..4667)
                     /locus_tag="P5056_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRY62590.1"
                     /translation="MMKKKKIISKTKSKKIPVSKKKVVKKVVTKAKLVKGKKAKPGKV
                     IVKKIKAKKPAEPWEKKADRLVEAGKKRRFVTFNEILKEFPNIEDDVLFLDELYDRLN
                     KLGIDVLDSGGLLNMGEDVDMYENKKKAGIRGEPALDSVQIYLREIGQHKLISAAEEK
                     ELARAIEKGDKEAMNSLARANLRLVVSIAKKFIGRSSDLTLLDLIQEGNLGLFKAVEK
                     FDWTKGYKFSTYATWWIRQAITRALADQSRTIRIPVHMVETIAKYKQVLRRLSQDLGR
                     DPLPSEIATEMGIEVEKVHVIEKIDQGTVSLETPVGNDDDNSSLGDFIADDKIISPDN
                     QTARRILSDQIKEILDDLTPKERKILEMRHGLVDGVMRTLEEVGKEFGVTRERIRQIE
                     AKAHEKIRDHEKANKLLGY"
     gene            complement(4677..6404)
                     /gene="dnaG"
                     /locus_tag="P5056_02370"
     CDS             complement(4677..6404)
                     /gene="dnaG"
                     /locus_tag="P5056_02370"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HRY62591.1"
                     /translation="MSSAVEQIKARLSIVDVVGSYIKLEKAGANYKARCPFHNEKTPS
                     FYVSPGRESYHCFGCDKGGDIISFVEDLEGVDFMAALKMLAEKAGVRLDNEDKYEKSE
                     KGRTAALLNSVIEFYRKELVKNADALNYLKGRGLKGETVTKFKIGFSPDDWRATGNYL
                     KKQGFTEDEIEKAGLSIKSPSGSYDRFRGRIMFPVYNISGSPIGFSGRIFDAKKTGGK
                     QEPAKYINTPQTVVYDKSRALFGIDKAKVEIRKKNECILVEGQFDLIMAHQAGTENSV
                     AVSGTALTEEHLTMINRLAEKLIMAFDGDSAGLKASARAIGLALSLGMEVRLVSIPGA
                     KDPADLIKERPDEWLKIVSGSKHIVDFFLDSLLNQKLNKDAFPKEVEKQVLPYVASIK
                     SKIEQAKFVNKISELLKISEDAVWEEVKKAAKLAETNSDTVAKKENFVKTTDENTKEF
                     NLKKMVWSFIEWQKDSGALDIKEREQRFRTLIKDSEYEKINSFLSPKRNQLILEVEIP
                     FAGATNLEKEIDEALRSLEEEIIKGEVSSEWMKIKEAEKTGDADKVSEHMSNFQKLSK
                     KLDDFKKRG"
     gene            complement(6501..10127)
                     /gene="rpoC"
                     /locus_tag="P5056_02375"
     CDS             complement(6501..10127)
                     /gene="rpoC"
                     /locus_tag="P5056_02375"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HRY62592.1"
                     /translation="MNEEQKIADFNSIMLRLASPEKIKEWSHGEVTRAETINYRTQRS
                     ERGGLFDERIFGPEKDYECYCGKYRRIRYKDIVCEKCGVEVTRSIVRRDRMGHIELAA
                     PVAHIWFLRGIPSRIGLLLDLSIADLEKVIYFAGYIITDVNEAEKDRVLKELDKEFKQ
                     KMKTVNSDEAKDKIKESLLEVKREINSLRPGLVLDEPTHIKYTYKYATIFESDIGAEA
                     VYKMFQKMDLAELIKKIEEKIETTSSADKAKLHKRLNLAMHMHKAGVRPEWMFLTVIP
                     IIPPALRPMVALDGGRHATSDVNDLYRRVINRNNRLKKLIEINAPDVILRNEKRILQE
                     AVDALIDNSIRHGSSAAGAMSSAQRRPLKSLADILKGKQGRFRQNLLGKRVDYSGRSV
                     IVVGPELHLDQCGLPKHMALELFRPFVISKILEKELAYNIRGAGRLIEDAVPEVWAIL
                     EEVIKNKYVLLNRAPTLHRLGIQAFMPVLIEGNAIRLHPLVCSAFNADFDGDQMAVHV
                     PLSDEAQKEAKELMCSAKNLLRPGTGDPIVTPKLDIVLGCYWMTKSIDGEKGEGKMFP
                     SPNAAISAYDYGQVGFRAKIKIMGGESEKYREFDGKIFETSVGKILFNSVLPSDFRYI
                     NHEVTGKEMPALIEELIEVYGTDAVPSILDKIKSFGYKYMTMSGITWGMNDVKVPAEK
                     KDIVKKAKTAVIEIGKNFDEGLISSEERYKMIISTWRSAWEEIQKLVLGTLDINGGVR
                     DMVISKARGSIAALSQMCGMKGLIINTSGKTLEFPVIPSYKEGLSPIEYFLTTHGSRK
                     GLTDTALNTSKAGYLTRRLVDVSQDVVVSVEDCGDKEGRTLYAKNILETSLGNAVRSR
                     VIAKDILDKDGKVVFKKGHLVTKKDAKIIEESGATEATVFSPLTCKAVQGICQKCYGI
                     DLGRNHLVSLGEAVGIVAAQAIGEPGTQLTMRTFHSGGVSEASGDITMGLPRIEEIFE
                     KRAPKNAAVVAMVDGVVSEIKNDGKEKVIIVMGQGGKKSEGSVEHPVPFTRNIIVRVG
                     NEVKKGDILTDGSANINELFTFGGKAVAEDYILNEVSKIYSMQAAAISRKHIEVILRQ
                     MFSRKKIVEAGDTDFVEGEVVENQELLRANDKTTGAKAKASDMVLGISEVSLTTSSWL
                     SAASFQNTSRILIDASTQGNTDELRGLKENVIIGRLIPAGTGFRKKDNYEEAEWAKKD
                     EEDK"
BASE COUNT         2619 a         2529 c         1848 g         3175 t
ORIGIN      
        1 tacacaaata atttcgcgac cgcgaaatta tttgtgtagg gtaaaaataa acaatctaaa
       61 aaaatattaa aatataaaaa agcgcctggg attaccaggc gcgagaacga caaaactact
      121 ttttatcacg cttcaagaga cgaaccgtat cggtcttttc atcgtaagaa aagatttcgg
      181 aaaaccactc aataaaattt agaataaagt atcgattgta ttcatcgata tccataaatc
      241 ctcggtctcc catatcgacg attggctttt cgtgcggctt aatgagcttg atgtcgtcca
      301 gcttcatctc tccgccgttc ttttttaaaa ggtcgagtaa ttctttttca atgggtctca
      361 ccgtaaatag cggctttttc tttttcccca taacgcatcc cctttctcaa tgaaccaatt
      421 tcgtatccca agacagccta acaaaatgaa ccataaaagc aaggtatgct ctggattccc
      481 gccttcgcgg gaatgacata attagtataa ttgtaactac tctctaccat ccaccctcca
      541 ctttctactc atatgatttt gcctgaagct gatacagctc cgcataaacc cccttctttg
      601 ccatcagctc ttcgtgagtt ccatcctccg ctttttcacc gtcttttatg actacaatct
      661 tgtgagcctt tctgacagtc gagaatctgt gagaaataag tataacgctt ctgttgtcgg
      721 aaacatctcc aatcttttca aatatattgg cttcagacag agcgtctatt gatgatgtcg
      781 gttcatcaag tatcataact tttggatcgc gatagaagac ccttgcgagc gccaatttct
      841 gccattgacc gacagacggc tcaactcctt ctgtgaacgc acgtccgagt gtctggctat
      901 atgccttgtc ccatttttca ataaagatat ccgcctcgct cgccttcgca gcatctttca
      961 ccttttcaat atttggcttg tcgcctgtcc taccgacggc tattgcctct tcgactgtca
     1021 gatgatacct ggagtagtcc tgaaatactg cgccaagcat gtgataccag ctatcaaggt
     1081 ctatttcacg caaatcatga ccgtttatga gtatcctacc ttcggtcgga tcataaaatc
     1141 tacaaagcaa tttcacgaat gtggtcttcc ctgcgccatt aagtcctaca agcgcaattt
     1201 tctctcccgg ctttattaca aatgaaaaat ttttcagcac cagtttatct gttcccggat
     1261 aagtaaaaga tacgttctca aaaacaactt caggggcaac ttttttatcc aaaactattc
     1321 ctttttccgg ctttttgata acggttttaa ttgaccaaaa agtaagtaaa tctctcacga
     1381 acaggccgtc tttgtactga cggccaaaca accagaaaca ccggctcagt tctctcttta
     1441 ggctttgcac ggaatataga acaaaggtta gtgtcccgat accaataatt ccgctggctg
     1501 tctgcatggc atatgatacg attgcgatag ccaaggcgac ctgcgataaa ccgactgcga
     1561 aaaattgaaa aattgcattt ctaatttcat ttttcttctc tgcaacctta aaatcatcaa
     1621 acatcttttt catcatttgg tagaaaaatc ccgcgctctg gaaaatattc aattccacta
     1681 aagaagactg gctaagaaaa agatcgcgaa tatcccaata tcttcttctt acttcaccct
     1741 ttgtgtcaga aataccccac accctagtac tgtaatatat tccattaaca agttctggaa
     1801 tggtagcaaa gaaaacgagt aaaaatatct tccagtttgc ccaaaacaac acggctgacg
     1861 caataattac catacccacc gagtccaaca ttaaaaattg tctgaagacg aattccgccg
     1921 aagaccatgc gcccgactca gcgattctat taaaaagatc cttaaactta ggatcttcgt
     1981 gaagggaaat atctatttcc gagctctttt taataataag taaatttatt tttctctctg
     2041 caatccaata caacataacc tcgcaaaatg aatctaggct cgacattaag gccggtatca
     2101 caatagacgc caccaacaaa ccaagggcta aataaaagga attcaaaacg atgtgcccgc
     2161 tggcaaccac ggctaattca tttaaaagca acgccagagc gcctgatgcc aaaaatggct
     2221 cagttgatgt aaaaattcta aagaaaaaaa gggcaaccag ataaaccttg gcatcggacc
     2281 acattacctt atccatcttc cacgcattca agacaagcaa ttcgtaactt tcgcgtattt
     2341 tcgaaaatat ttttttcatt gggatattat aacacaaaca cggattcccg cctgcctgcg
     2401 caggcaggcc ttcgcgggaa tgacaaagtt atgcgccgac tatgtggccc caaactattt
     2461 gtgaacgatt aaataccaca agaaagtcga gattgccaaa ataaataatt tgtctcttgt
     2521 gtcgctcagc aagaagctca aaaacttgtt atgtttttta acagcctttc cgtcgccctt
     2581 aaatttgtga atttcttctt tcaacttttc agcgtttgcc acaatatgaa gatcattatt
     2641 tctggcgata gttattgacc ctctttcctc cgacacgatt attatcattg cgtctgttct
     2701 ctcggcaagc cctaatccgg ccctgtgtct cgtgcctatg ccgggatatt ttttgttcat
     2761 atcggcaagc ggtaggtgca ccataaatct tttcacaatg tcaccttcta tcgtcaccgc
     2821 accatcgtgg cccggtgttt ttgggtcaaa aatagaaaga agtaggggct cggatatctt
     2881 tccgttaagc ggcgtccctc cttcgagtac cctctccaag ctttgcttgc ccggaagaac
     2941 tatgagcgcg cctctccttg tcttggaaag ctcagcaacc acattgcaga taatttcgat
     3001 aacattgtcg ctgacagagg cctcttcagc aaaagcgcta acgaagaaat caatagtgaa
     3061 attttcgaag aatcgtctta tctctttctg gaaaaggatt acaagaataa tgatgaagaa
     3121 ggaaataaac gcctggaaaa gagatgttgt cagcttaaga ttcaagaaca cggcggcaat
     3181 gtaaaaaaga cccaggccga agaaccctct caatatatag atagagcggg tttttttaag
     3241 caaaaccagc gccgtgtaaa taataaaagc ggtggttaat atgtctaaaa tttctaaaaa
     3301 tattgtcaac ataaattaaa aagtttttag tttatagtat ttagtgactg ccaactacta
     3361 tctacaaact acaacctaga ctctggatcc ccgaccaaat cggggatgac aggtattggg
     3421 agcgaatctc cttaataacc taacaactta ttggcctttt cgtgatctct tattttctcg
     3481 tgagccttgg cctcgatctg cctgattctt tctctcgtaa ccccgaactc ctttcctact
     3541 tcttcaagag ttctcataac cccatctaca agtccatgcc tcatctcaag tatctttctc
     3601 tcttttggag taaggtcgtc caatatttct tttatttgat ccgaaagaat tcttctcgct
     3661 gtctgattat ccggtgagat tattttgtcg tctgcgatga agtctcccaa tgatgaattg
     3721 tcatcgtcgt ttccgacagg ggtctcgagc gaaactgttc cctgatcaat cttttcgata
     3781 acatgcacct tttcaacctc gatacccatc tctgtagcaa tctctgaagg aagagggtct
     3841 ctgcccaagt cctgagaaag tcttctcaaa acctgcttat actttgcgat cgtttcaacc
     3901 atgtgcacag gaatacgaat ggttctcgac tgatctgcga gggctctcgt aatcgcctgt
     3961 cttatccacc atgtggcgta tgtagagaat ttatatccct ttgtccaatc aaacttttca
     4021 accgctttaa agagtccgag gttaccttcc tgaataaggt cgagaagcgt gagatccgag
     4081 cttctaccga taaatttctt tgcaatagaa acaacgagtc ttaagtttgc tctggcgagg
     4141 gagttcatcg cctccttgtc gcctttttcg atagctctgg ccaactcttt ctcttccgcc
     4201 gcagatatca atttgtgctg accgatctct ctcaaatata tttggactga atcaagagcc
     4261 ggctcacctc ttattccggc cttcttcttg ttctcataca tatccacgtc ttctcccata
     4321 ttgagaagcc ccccgctatc caagacgtct atgccgagct tgtttagcct gtcataaagt
     4381 tcgtccaaaa agagaacatc atcttctata ttaggaaact ctttcaatat ttcgttgaaa
     4441 gtaacaaatc tcctcttttt gcccgcctcc acgagtctgt cggctttttt ctcccatggc
     4501 tctgccggtt tcttcgcttt tatcttcttt acaattacct ttcccggttt cgccttcttg
     4561 cccttaacaa gctttgcctt agtgacaact tttttcacca ctttcttttt tgaaacaggg
     4621 atttttttgg atttagtttt tgaaattatt tttttctttt tcatgatttt tgaaaattat
     4681 ccccgctttt taaagtcatc caacttcttt gaaagcttct gaaaattact catatgctcc
     4741 gacaccttgt ccgcgtcccc tgtcttttca gcctccttta tcttcatcca ctcggagctt
     4801 acttccccct ttataatctc ctcctccaga gaacgtaacg cctcgtctat ttctttttcc
     4861 aaattagtgg cgcccgcaaa aggtatttcg acttcaagta taagctgatt cctctttggt
     4921 gacaagaaac tatttatttt ttcatactca ctatctttta taagcgtcct gaatcgttgt
     4981 tctctctctt tgatatccag ggcgccggag tccttctgcc actcgatgaa agaccaaacc
     5041 atttttttga gattaaactc tttcgtgttt tcgtccgttg tttttacaaa attctctttt
     5101 ttggctaccg tgtccgagtt cgtctctgct aatttagccg ctttcttcac ctcttcccac
     5161 acagcatctt cacttatttt caaaagttcg gatattttgt ttacaaattt ggcctgctct
     5221 atcttactct ttatactagc cacatacggc aaaacttgtt tttctacttc tttgggaaaa
     5281 gcgtctttgt ttaatttctg attgagaagc gaatccaaga aaaaatctac gatatgcttt
     5341 gatccggaaa ctattttaag ccactcgtca ggcctctctt ttataaggtc agccggatct
     5401 tttgcgccgg gaatagatac caatcgcacc tccataccca gcgaaagagc gagaccaatc
     5461 gccctggccg aggccttcag ccccgccgaa tctccgtcaa aggccataat aagcttctct
     5521 gcgagcctat ttatcatggt tagatgctct tctgtaagtg ccgtgcccga tacggcgacc
     5581 gaattttctg tccccgcctg atgcgccatt atgagatcga attgtccctc gacgagaata
     5641 cactcgttct tttttcttat ctctacttta gccttgtcta ttccaaagag cgctcgggac
     5701 ttgtcgtata caaccgtctg aggagtattt atatatttcg ccggctcttg tttcccgccc
     5761 gttttttttg cgtcaaatat tctcccggaa aaacctatcg gcgaaccgga aatattataa
     5821 acagggaaca ttattcttcc ccggaatctg tcataagaac cgctgggcga ttttatagac
     5881 aatcccgcct tttcaatttc gtcttctgtg aatccttgct tcttcaaata attaccggta
     5941 gctctccagt cgtccggaga aaaaccgatc ttaaatttag tgacagtctc cccttttagg
     6001 ccccttcctt ttaagtaatt aagcgcgtcg gcattcttta cgagctcttt gcggtaaaat
     6061 tctatgactg aattaaggag cgcggctgtt ctgccctttt ccgacttttc atatttatcc
     6121 tcattgtcta gccttacacc cgctttctcc gcaagcatct tcagggcggc cataaaatca
     6181 accccctcaa gatcctcgac aaaactgatt atgtctcctc ccttatcgca accgaagcag
     6241 tgatagcttt ctctaccagg agacacataa aacgaaggtg tcttttcatt atggaaagga
     6301 cacctcgctt tataattcgc gccggctttc tctagcttga tgtaagaacc taccacatca
     6361 acgatgctga gtcttgcttt aatttgttct acagcggagg acataaaaag ttatcaagtt
     6421 aaaatgttat aaagttataa tgtttttgtt ttttgttgtt tttcttaacc ttataacttt
     6481 caactttaaa actttacaac ttacttgtcc tcttcgtcct ttttggccca ttcagcctct
     6541 tcgtaattat ctttctttct gaatccggta cctgcaggaa tcaatctacc tatgataaca
     6601 ttttctttca aacctcgtag ctcgtccgta tttccctgcg tagacgcatc gataagaatt
     6661 cttgatgtat tctggaagga agcggcggac aaccagcttg atgtcgtcaa agatacttct
     6721 gagattccca ataccatatc cgaggccttg gcctttgctc cggtcgtctt gtcgttggct
     6781 ctcaaaagct cctgattttc cacaacctct ccctcgacga agtctgtatc tccggcctca
     6841 acgatcttct ttcttgagaa catctgtcgc aagatgactt cgatgtgctt tctcgagata
     6901 gcggcggctt gcatagagta gatctttgaa acttcattca agatatagtc ttcagcaaca
     6961 gccttacctc cgaatgtgaa caattcgtta atgttggcgg aaccgtcagt caaaatatct
     7021 cctttcttta cctcattgcc gactctaacg ataatatttc ttgtgaatgg aacaggatgt
     7081 tctacggaac cctccgactt ctttcctccc tgacccatta ctatgattac cttctctttg
     7141 ccgtcgttct taatctcgga aacaactccg tcaaccatcg cgacaacagc ggcgttcttt
     7201 ggcgctctct tctcgaagat ctcttcgatt ctagggagac ccatagtgat atctcctgat
     7261 gcttcggaga ctcctcctga gtggaacgtt ctcattgtga gctgtgttcc cggctctcca
     7321 atagcctgag cggctacgat acctacggcc tctccgaggc taaccaaatg gttccttccg
     7381 aggtctatac cgtaacattt ctggcagata ccctgaactg ccttacatgt aagtggcgag
     7441 aatactgtcg cctctgtggc gccggactct tctataatct ttgcgtcttt ctttgtgaca
     7501 aggtggccct tcttaaagac aaccttaccg tctttgtcca atatatcctt ggcgataacc
     7561 ctgcttctga cggcgttacc gagcgatgtc tctaatatat tctttgcgta aagagttcgt
     7621 ccttctttat ctccgcaatc ctcgacagaa acaactacat cctgtgacac atctacaagt
     7681 cttctggtca aataaccggc ctttgatgtg ttcaaagctg tatctgtgag accttttcta
     7741 gaaccgtgtg tcgtgaggaa gtattcgatt ggagacaatc cctctttgta agaaggaata
     7801 acagggaact caagcgtctt accggaagtg ttgatgataa gacctttcat accgcacatc
     7861 tgactcaaag cggcgataga acctctggcc tttgaaataa ccatgtctct gactcctcca
     7921 ttaatatcaa gagttccaag aacaagcttc tgtatctctt cccacgcgga tctccaagta
     7981 gagatgatca tcttgtatct ctcttcagaa gagatgagac cctcgtcaaa gtttttgcct
     8041 atttcaatga cggcggtctt ggctttcttt acaatatcct tcttctccgc agggacctta
     8101 acatcgttca taccccaggt aataccggac attgtcatat acttgtaacc gaagctcttt
     8161 atcttatcca aaatactagg aacggcgtct gtgccgtaaa cctcaatgag ctcttcaata
     8221 agagcaggca tctcctttcc tgtaacctcg tggttgatgt atctgaagtc tgaaggcaaa
     8281 acgctgttaa agagtatctt tcctacggag gtctcgaata tctttccgtc aaactctcta
     8341 tacttctcgc tttctcctcc cattattttg atcttcgctc ggaatccaac ctgcccgtaa
     8401 tcgtaagcgg aaatcgcggc gtttggtgaa gggaacattt ttccttctcc cttttctccg
     8461 tcgatagact ttgtcatcca ataacaaccg agaacgatat ccaatttagg tgttactata
     8521 ggatctcctg taccaggtcg gagcaagttc tttgccgagc acataagctc cttggcttcc
     8581 ttctgcgcct catcggaaag cggaacgtga acagccatct ggtctccatc gaagtcggcg
     8641 ttgaaagcgg aacatacgag agggtgaagt ctgatagcat ttccttctat aaggacaggc
     8701 ataaaggctt gaatacccaa tctgtgcaat gtaggagcac ggttcaagag aacgtatttg
     8761 ttctttataa cttcttccaa aatagcccaa acctcaggga cggcatcttc aataagtctg
     8821 cctgcacctc ttatgttgta agcgagctcc ttctccaaaa tcttggaaat aacgaatggt
     8881 ctgaaaagtt cgagcgccat gtgcttagga agaccgcact gatccaagtg aagctcaggt
     8941 ccgacaacga taacgcttct tcctgaatag tcgactcttt taccgagcaa gttctgcctg
     9001 aatcttccct gctttccttt caaaatatca gcgagtgact tgagaggtct tctctgggcg
     9061 gagctcatcg ctccggcggc ggaacttccg tgtctaatcg agttatcaat aagagcatca
     9121 acagcctcct gaagaattct tttttcattt ctcaaaataa cgtcaggagc gtttatctcg
     9181 atgagcttct ttaatctgtt attcctgttt ataactcttc tgtaaagatc gttaacatcg
     9241 gatgtcgcgt gtcttccacc atcgagggca accattggtc taagcgcggg cggaataata
     9301 ggaatgaccg taaggaacat ccactccggt ctaacgccgg ccttgtgcat gtgcatggcg
     9361 aggttaagcc tcttgtgaag cttcgccttg tctgctgagc ttgttgtctc gatcttctct
     9421 tcaatctttt ttataagttc ggcgaggtcc attttctgga acattttata cacggcctcg
     9481 gctccgatat ctgactcgaa gattgtcgca tacttatatg tatacttgat gtgagtaggc
     9541 tcatccaaaa cgagtcccgg tctcaaagaa tttatttctc tctttacctc aagtaaggat
     9601 tctttgattt tatctttggc ctcgtcggaa tttacagtct tcatcttttg cttgaattcc
     9661 ttatcaagct ccttcaagac tctgtccttt tcggcctcat taacatccgt gattatgtat
     9721 ccggcaaagt aaatcacctt ttcaagatcg gctatagaaa ggtcgagcaa gagtccgatc
     9781 cttgatggga tacctctaag gaaccaaatg tgagctacag gagcggcgag ttcaatgtgg
     9841 cccattctgt ctcttcttac gatagatcgg gtcacctcga ctccacattt ttcacaaaca
     9901 atatccttgt atcttattct tcgatactta ccgcaataac attcgtagtc tttttcagga
     9961 ccgaaaattc tttcatcaaa caaaccccct ctttcacttc tctgggttct atagttgatt
    10021 gtctcagctc ttgtaacctc tccatgagac cactccttga tcttctccgg ggaagcaagt
    10081 cggagcatta ttgagttgaa atctgcgatt ttttgttctt cgttcatggt gttggttctt
    10141 agttattttt aagctccaca tcaagtgcga g
//
LOCUS       DAOTEX010000028         9815 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3118
            NODE_4889_length_9815_cov_3.990779, whole genome shotgun sequence.
ACCESSION   DAOTEX010000028 DAOTEX010000000
VERSION     DAOTEX010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868142
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 9815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 883
            CDSs (total)                      :: 833
            Genes (coding)                    :: 829
            CDSs (with protein)               :: 829
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9815
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4889_length_9815_cov_3.990779"
                     /isolate="AMR_MDS_3118"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..406)
                     /locus_tag="P5056_02380"
     CDS             complement(<1..406)
                     /locus_tag="P5056_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62593.1"
                     /translation="MGCVNLSQTSKTAECIRDLVTLIYTAPRSDEYKKEEPKQKKGKK
                     EPVTKEEEEALMARPDVFVRMVGEDGTRKLLVGEFFSNADICGRLKLNVKFVSREGVC
                     TQFSHLPVENREIVLTTVRDIVVAVSEAQKRMA"
     gene            complement(552..1025)
                     /locus_tag="P5056_02385"
     CDS             complement(552..1025)
                     /locus_tag="P5056_02385"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HRY62594.1"
                     /translation="MGEHHETKIREVLINGLENAKQSIRLAVAYFNDLEIANIIKKKA
                     EEGIATEVIIYDHFVNSPAIIELNSNNIKTFFYRVYETQPIYPKFCLIDGDILVTGNH
                     GWAYAANRTEHPEPLIVNNENILIEEYINKFYSLKKNCGASKLVHFRIKPESPKF"
     gene            complement(1127..1456)
                     /locus_tag="P5056_02390"
     CDS             complement(1127..1456)
                     /locus_tag="P5056_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62595.1"
                     /translation="MSIISKFSVGDTVTSMFPNDPRGQVTIKIKEIRTDKSLLVEVVE
                     SSEFWNREFFGPPPLLRGMKIGASFVMEYRKDADGNEWWIPKNEENICCFVLELFEGK
                     KVFDFYK"
     gene            complement(1745..1815)
                     /locus_tag="P5056_02395"
     tRNA            complement(1745..1815)
                     /locus_tag="P5056_02395"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1781..1783),aa:Gln,seq:ctg)
     gene            complement(1989..2918)
                     /locus_tag="P5056_02400"
     CDS             complement(1989..2918)
                     /locus_tag="P5056_02400"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HRY62596.1"
                     /translation="MTWVNIKRVSKAGFVSFLRNGMTSLSAVLVMFISLFIITSLVLS
                     SALSSSIINMLKDKVDINVYFKSNATEEDILALKEKIELLPEVREATYVSSEESLANF
                     KERHKDNSLILSSLDQLKDNPLGAVLSIKAKEPSQYEGIAKFLEEDGVSISADGSGII
                     DKINFYQNKLVIDRLTRLISATEKVGFSISLVFALIAIIVTLNTMQLAIYTAREEISV
                     MRLVGAGNWYIRGPFIVSGVMAGLIASVLVLVIFYPITAWVGGKTADFLAGINLLNYY
                     TANFLQIAAITIGSGILLGAVSALLSVRRYLHI"
     gene            complement(3090..3770)
                     /locus_tag="P5056_02405"
     CDS             complement(3090..3770)
                     /locus_tag="P5056_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRY62597.1"
                     /translation="MIYLENLTKKYSDKTLALDDINLEISRGEFVTIVGHSGAGKTTM
                     LKMITAEEAPSLGKVFFESTNVHDLRKGDVPKYRRKIGVVFQDNRLIPNKTAYENIAF
                     AMEAAGRSDHEIVADVPEILKLVGLDNKLWNFPHELSGGERQRVSIARAVINQPELII
                     ADEPTGNLDPINTFDVINLFKKINEMGTTVLLTTHNKGVVDLIGNRVVTVEKGKIIRD
                     DKDGKYVL"
     gene            complement(3893..4804)
                     /locus_tag="P5056_02410"
     CDS             complement(3893..4804)
                     /locus_tag="P5056_02410"
                     /inference="COORDINATES: protein motif:HMM:NF012684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HRY62598.1"
                     /translation="MSEVEIEQKIKELEAEMQKYDFWENKLRAQEVLKELSRLKDEAL
                     GARKYDRGDAIITIFSGAGGDDAEDFSRILFEMYSKYINKKGWSMYILHQNENDHGGY
                     RNITFEVSGKDSYGTLKNESGVHRLVRVSPFSAKKLRHTSFSMVEVIPKFKKGEAIDI
                     PETEIEIEFSRAGGPGGQNVNKRETAVRVVHMPTGISAHSEAERSQLANKDKAIDILR
                     GKIFKYLEEERIKKEEGLYVSKITSAEWGSQIRSYVEHPYKMVKDHRTGAETSDIDAV
                     LDGNLDEFIEAEKDFDSKKKHELGIKN"
     gene            complement(4959..5031)
                     /locus_tag="P5056_02415"
     tRNA            complement(4959..5031)
                     /locus_tag="P5056_02415"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4996..4998),aa:Thr,seq:cgt)
     gene            complement(5207..5279)
                     /locus_tag="P5056_02420"
     tRNA            complement(5207..5279)
                     /locus_tag="P5056_02420"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5244..5246),aa:Thr,seq:ggt)
     gene            5475..6161
                     /locus_tag="P5056_02425"
     CDS             5475..6161
                     /locus_tag="P5056_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62599.1"
                     /translation="MSKFEQRIRAKKLRTEGMSLGDIAKNLGVSKGTVGLWCRDIILT
                     KEQESLLKQKVIRLGLTGRMKGAEMNKKSKESRIELAKKDSEDRLSKLSEKDLFVCGL
                     SLYWAKGSKSPSSRFCFTNSDPAMIKIFLNFLYKIYNVQKKAIMPKIAINETHRDREK
                     IVLKYWSNLLGIPLGQFRNTSFNKTKIKKIYDNHDKYFGTMSIRVEKSNQLWYKILTS
                     IDIIKNRMPA"
     gene            6152..6223
                     /locus_tag="P5056_02430"
     tRNA            6152..6223
                     /locus_tag="P5056_02430"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6184..6186,aa:Met,seq:cat)
     gene            6270..6343
                     /locus_tag="P5056_02435"
     tRNA            6270..6343
                     /locus_tag="P5056_02435"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6304..6306,aa:Asp,seq:gtc)
     gene            complement(6761..6898)
                     /locus_tag="P5056_02440"
     CDS             complement(6761..6898)
                     /locus_tag="P5056_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62600.1"
                     /translation="MKYFDKDFWKFAGQFFLVILFVLITLSLLSGYLSITGGEANIMG
                     N"
     gene            complement(7055..7642)
                     /locus_tag="P5056_02445"
     CDS             complement(7055..7642)
                     /locus_tag="P5056_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62601.1"
                     /translation="MEILGKLFGSENRVKIMRLFLIGPEITFIKEDVSKRCRVSKESA
                     LKELNLLKKIKFLEDKKIKQEGNKKSVVGLKLNQDFVYIDSIKELLLGSDIAKGDDLA
                     KKMRGIGKVKLVVTSGIFMDNENSPADLLVVGDSIKRKTLSNIIKTIESEIGREIRYS
                     VLDTEDFKYRMTVYDRFVREIMDLPHNKIIDKLYE"
     gene            complement(7946..8326)
                     /gene="rplL"
                     /locus_tag="P5056_02450"
     CDS             complement(7946..8326)
                     /gene="rplL"
                     /locus_tag="P5056_02450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HRY62602.1"
                     /translation="MEVPAKFKDIVEKIETMSVLDLSELVHVLEEKFGVKAQAAVMAA
                     APAAAAGEAAAEKSSFNVELTAGGDNKIGVIKVVKEVLGLGLKEAKDLVDKAPAMLKE
                     GMDKAAADALKKQIEDAGGKVTLK"
     gene            complement(8351..8872)
                     /gene="rplJ"
                     /locus_tag="P5056_02455"
     CDS             complement(8351..8872)
                     /gene="rplJ"
                     /locus_tag="P5056_02455"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HRY62603.1"
                     /translation="MAITLQKKKEIIESLNDKLKDAGSAVIVGFKGLKIADITPLRKT
                     LRDGGVSFSVAKKTLFKRAFNDVKIEGNLPPMDGQVALAIGKDPIAPAKSIAEAQKKY
                     KDAVYILGGVLEGKFMSAAEMIALSKIPGREVLLSQLLNVMQSPIQGFVGTLNAVTRD
                     FVCTLDQVAKSKN"
     gene            complement(9381..>9815)
                     /locus_tag="P5056_02460"
     CDS             complement(9381..>9815)
                     /locus_tag="P5056_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62604.1"
                     /translation="ERARPPGSGARRHGSGPRSRRLRHPLDGPQPDALGTAVGGDLVL
                     LLLEAVANVRVDRGQPVVEGLEVGEEDGVEVADLLFQEELALLDPCFKYKDEFLATHG
                     LSPWIGMYVVELFTDKKGRLTQVIANYRNYRPYAISLLENKK"
BASE COUNT         2847 a         2275 c         1795 g         2898 t
ORIGIN      
        1 ccgccattct cttctgcgcc tccgagacgg ccacgacgat gtcgcgaacg gtcgtgagaa
       61 cgatctctcg attctcgacg ggaaggtggc tgaactgcgt gcaaactcct tctcgggaga
      121 cgaacttaac gttcagcttg agacgaccgc agatgtcggc attcgagaag aattctccga
      181 caaggagctt cctggtgccg tcttcaccca ccatccgaac gaacacgtcg gggcgcgcca
      241 tcaaggcttc ctcttcctcc ttggtcacgg gctccttctt gcccttcttc tgtttgggct
      301 cctctttctt gtactcatcg cttctggggg cggtgtagat gagggtgacc aaatccctaa
      361 tgcactcggc ggttttggaa gtttggctga ggtttacgca acccatccga tttccccttt
      421 cacagacgta tgtatttttg ttaaggtccc acaggaacta catatatatc tatcataaac
      481 gagaattttt gtcaactcat ttacgggcaa gcaaaaaccg ccccacttgg gtggccggcg
      541 gttttcaaaa attaaaattt ggggctttcc ggctttatgc ggaaatgaac cagtttactg
      601 gcgccacaat tcttctttaa ggagtaaaat ttatttatat attcctctat taggatattt
      661 tcgttgttca cgatgagagg ctcagggtgt tctgttctat ttgcggcgta agcccagccg
      721 tgattacccg taaccaaaat atcaccgtct atcagacaga atttaggata gatcggctgt
      781 gtctcataaa ctctataaaa gaatgttttt atattattgg aatttagctc tattatcgcc
      841 ggactgttta caaaatggtc gtaaattata acttcagtag ctatcccctc ttccgccttc
      901 tttttaataa tattggctat ttcaaggtca ttaaaataag cgacagcaag gcgaatagac
      961 tgcttggcgt tttccaaacc gttaattaaa acctctctaa tttttgtttc atgatgttct
     1021 cccatatgtt tcgtaagttt taaatactaa ggaacaatac tagatttata gtaaattggc
     1081 aaggggtaaa aagataaaag aaaacctccc cgtagggagg ccagatctat ttataaaaat
     1141 cgaaaacttt ctttccttca aataactcaa ggacgaaaca acatatattt tcttcatttt
     1201 tgggaatcca ccactcattg ccatcagcat ctttgcggta ttccataacg aaagacgctc
     1261 caatcttcat cccgcgcaag aggggtggcg gcccaaagaa ttctctattc cagaattcgc
     1321 ttgattcgac cacttctact aaaagcgatt tgtcggtacg aatttcttta atcttgatag
     1381 taacctgacc acgagggtca ttcggaaaca tgctggtaac agtgtctcca accgagaact
     1441 tagaaattat ggacattttg ctcccccctt caagttcaaa gaactgatct ttattaatac
     1501 caaaatatca tagcaaatta tagcagtcaa agttaatagc gggacttgtt tcggccacga
     1561 gttactaagt ctgctccata ttgcttcgca atattgcgcg gactaagtac tctcggcccc
     1621 gcctcggcga acgtgcgcca cttcgctacg tcggcgacgg tgcccgccct gcggccttct
     1681 catccaatcc cgacagaaaa cataaaaacc tcgatgctaa cgcatcgaga gttttatgtt
     1741 tttactgcgg gacttggatt cgaaccaaga tagcatgctc cagaggcatg agtcctacca
     1801 ttagacgatc ccgcaatgta tttttatttc aacacacttt catgctccag aggcatgagt
     1861 cctacttcac cccgccagtc ccgccatcgc gggataaagc ggggcaagtt agacgatccc
     1921 gcaatattta attaaaacaa ccgtgctatg aatataacac agttgcgtat tttttcaata
     1981 taaaaaagct aaatgtgtaa ataacgcctt acggaaagaa gggcggaaac agcacctaaa
     2041 agtatccctg agcctatggt aatagccgct atctgtagga aattggccgt ataataattc
     2101 aaaagattta ttccggctaa gaaatcagcg gtcttccccc ctacccacgc cgtaatcgga
     2161 taaaagataa ctaataccag aacggaggcg ataaggccgg ccataacgcc cgatacgata
     2221 aacggtcctc taatatacca gttacccgcc ccaacgagcc tcatgaccga aatctcctct
     2281 ctggcggtat aaatagccaa ctgcatagtg tttaacgtaa caataatagc gattagtgca
     2341 aatacaagtg agatagaaaa cccgactttt tccgtggcgg agataagcct tgttagtcta
     2401 tcgataacca gcttattctg ataaaaattt attttgtcga ttattccaga tccatccgct
     2461 gaaattgaga cgccatcctc ctccaggaac ttagcaatcc cttcatattg agaaggctct
     2521 ttcgctttta tgctcaaaac cgcacctaaa ggattatctt ttagttgatc caatgaggag
     2581 agaataagtg agttgtcctt gtgcctttct ttaaaattag ccagcgattc ttcactactt
     2641 acataggtcg cctctctcac ttccggcaag agctctattt tttccttaag ggccagaata
     2701 tcttcctcgg tagcgttgct cttaaaatat acattaatat ctaccttatc tttcagcata
     2761 tttataattg aagaagaaag agcactgctt aagacaagag aagttattat aaaaagagag
     2821 ataaacatga ccaaaacagc cgacagggac gtcatgccat ttctcaaaaa acttacaaat
     2881 ccggccttag atactctctt tatatttacc caagtcatat aaataaatta ccaattatca
     2941 attacgaatt actaattatt gttttaaata taaatatttt aaatcttaga cgtagtttat
     3001 actacccatc cactgctcat taaaaaataa tttttagacc tttggttgtg tttcaaattc
     3061 gtaattagta attattaatc tttaattttt taaagcacat acttaccgtc cttgtcgtcc
     3121 cttataatct tccccttttc tacggtcaca accctatttc cgataagatc caccacgccc
     3181 ttattgtggg tcgtcagaag aaccgtcgtg cccatctcgt ttatcttctt aaataaattt
     3241 attacgtcaa aggtattaat aggatccaaa ttccccgtag gttcgtcggc tataataagc
     3301 tcgggctgat ttataaccgc tctggcgata gagaccctct gcctctcccc gcctgaaagc
     3361 tcgtgaggaa aattccaaag cttattgtcc aaaccaacca atttcaaaat ttccggcaca
     3421 tccgcaacga tctcatgatc gcttctcccg gcagcttcca tggcaaaagc gatattttcg
     3481 tatgcggttt tgtttggaat aagcctattg tcttggaaaa caacccctat ctttcttcta
     3541 tatttaggga cgtctccttt tcttaagtcg tgaacgtttg tggattcaaa aaaaactttt
     3601 ccaaggcttg gggcctcttc tgcggtaatc atcttcagca tagtagtctt gcccgcgccg
     3661 gagtggccga cgattgtaac aaattcccct ctacttattt ccaaattgat atcgtccagg
     3721 gcaagagttt tgtccgaata ttttttagtt aagttttcta gatatatcat aaaaatgaat
     3781 caagaataaa gaattatgaa taaagggcac aaattacaaa aatacgtatt cttattatac
     3841 tcgaaaaaac aataaacaaa aaacggtgta taaggcaatt gtcgagagac tcctaattct
     3901 tgattcctaa ttcgtgcttc tttttagagt caaaatcttt ttcagcttct ataaattcgt
     3961 ctaaatttcc gtctaaaacg gcgtctatat cgctagtctc ggcgccggtc ctgtggtcct
     4021 taaccatttt atacggatgc tctacatatg accgtatctg acttccccat tccgcggaag
     4081 ttatcttgct gacataaagc ccctcctctt ttttaatgcg ttcttcctct aaatacttaa
     4141 atatttttcc gcgcaaaata tcgatcgctt tatccttgtt cgcgagctga gaacgttcgg
     4201 cctccgaatg cgccgagata ccggtcggca tgtggaccac ccttacggcg gtttctcttt
     4261 tgttcacatt ttggccaccc ggcccaccgg ccctagaaaa ctctatctcg atttcagtct
     4321 cggggatatc tatcgcctcc ccctttttaa actttggtat tacttcaacc attgaaaatg
     4381 aggtgtgtct aagcttcttg gcggaaaatg gcgacacgcg cacaagtcgg tgcacaccgg
     4441 attcattttt tagtgtccca taagaatctt tgcccgatac ctcaaaagtg atatttctat
     4501 agccaccatg atcgttctcg ttttggtgca atatatacat actccacccc ttcttgttta
     4561 tgtattttga atacatttca aacaagatcc ttgaaaaatc ctccgcgtca tctccaccgg
     4621 ctccggaaaa tatcgtaatt atcgcatctc ccctatcgta tttccttgca ccaagagcct
     4681 cgtctttgag acgggaaagt tctttcaaaa cttcttgcgc gcgcagttta ttctcccaga
     4741 aatcatattt ctgcatctcg gcttcaagct cttttatttt ctgttctatt tctacttctg
     4801 acatttaccc tgttaagtaa aaatttgttt agttattttt acctaatatt atttaaaatt
     4861 cttaatattt tcgactatca tattacccca ataaatctca cattactaaa caaattttaa
     4921 atctttttaa aaagatttta cttaacaggg tgaatctgag ccggagaccg ggcttgaacc
     4981 ggtgacctaa ggtttacgat accttcgctc taccaactga gctactccgg caataatggt
     5041 gagccattgt ccgggtattt cactcctggg aaacccacgg ttttcccaat ctttcctcca
     5101 cggggaaaaa tacttttccc cgaccccctt cgagttcaat ccctaaccaa gactcactat
     5161 ttgatacatc tcaaaaacca caatatctat ctaaaaatac ttcgtgagcc attgtccggg
     5221 attgaaccgg agacctcgtc attaccaatg acgtgctcta ccaactgagc tacaatggca
     5281 cgctatgctt tattttatca ttgaacacca aacgaccatc atataattaa ataccacttc
     5341 tcgccccgtg gcgaagccgc cttggggagc tacaatggct taaatttaaa tgtatctagt
     5401 catattagta taacgtattc attttttcaa ttatattgta aacatactga cgatataata
     5461 taatatatat aattatgtct aaatttgaac aaagaatacg agctaaaaag ctaagaactg
     5521 agggaatgag tcttggtgac atcgcaaaaa atcttggggt atcaaaaggt accgttggct
     5581 tatggtgtag ggatattatc ctcacaaaag agcaagagag tctcctcaaa caaaaagtta
     5641 taaggctagg actgacagga agaatgaagg gggcagaaat gaataaaaag agcaaagaga
     5701 gccgcataga actggcaaag aaagatagtg aggatagact ttcgaaacta tcagaaaaag
     5761 acttatttgt ctgcgggttg tccctctatt gggcaaaagg tagcaaaagc ccatcttcaa
     5821 gattttgttt cacaaattct gaccccgcaa tgattaaaat atttttaaat tttttatata
     5881 aaatttataa tgtgcagaaa aaggcaatta tgcctaaaat agctataaat gagactcacc
     5941 gcgacagaga aaaaatagta ttaaaatact ggtcaaatct tttaggtatc ccattgggac
     6001 aattcagaaa tacgagtttt aataaaacaa aaattaagaa gatatatgat aatcacgaca
     6061 aatactttgg gacgatgtcg ataagagttg aaaaaagtaa ccaactatgg tacaaaatac
     6121 tgacaagtat tgatataata aagaatcgaa tgccggcgta gctcagtggt agagcacatc
     6181 actcataaag atgtggtccc aggttcgatc cctggcgccg gcacattgga ctaaagagtt
     6241 caatattgtt atatttgtag agcaaaagtg cggtcgtagc tcaactggtt agagcactcg
     6301 cctgtcacgc gagaggttgc cggttcgatc ccggtcgacc gcgtacaaaa actccccatg
     6361 ggagtttttt gctttctagc ggtcgaccgg gatcgaacgg gaaaggggtc gggaaaactc
     6421 tagttttccc gtgaaggaag gaatggggaa accgtgggtt tcccagtgcg tgagattgcc
     6481 cggtcgaccg cgcaggaaaa atcacccaaa tagggtgatt ttttgttcaa aaagaaaaac
     6541 gccttatggg cgttcttctt aactttagcg ggggcacaga gtcgcactgg cttctcaagg
     6601 ttatgagcct tgcgaggtac tcttcctcca ccccgcttta tgtccaaata ctatactcct
     6661 tttgtaaggt tagtgtcaat gctcccccag aaggaccctg ttaaataatt tgccaaagca
     6721 aattagcgct aagcgctatt taacagggcc acggggtaaa tcaattaccc atgatattgg
     6781 cctcccctcc cgtaatagac aaatatccgc taagtaggga aagagtaata agcacaaaca
     6841 agattaccag aaaaaattgt ccggcaaact tccaaaaatc tttatcaaaa tatttcatcc
     6901 tgtagtagaa ttttatatca ataatttacg taacaacttc gcgaaaaata atcactccct
     6961 ataatcaata ctgagatatg aaaaacaaaa tttcactact ggatttcata taagtaaatt
     7021 atagcacacg aagcaccttg tggggcagac gaaattactc gtataatttg tctataattt
     7081 tgttgtgagg cagatccatt atctctcgca caaatctgtc atatacagtc atcctgtatt
     7141 taaaatcttc agtatccaat acgctatatc ttatctctct gccgatttca gattctatcg
     7201 ttttaattat atttgataga gtttttctct tgatactatc cccgacgaca agaaggtctg
     7261 cggggctgtt ttcgttatcc ataaatattc cggaagtcac gacgagcttc accttgccta
     7321 tccccctcat ctttttcgcc agatcgtccc cttttgcgat atcagaaccg agaagcaact
     7381 cttttatgct atctatataa acaaaatcct gatttaattt taaaccgaca acactctttt
     7441 tatttccttc ctgtttaatc tttttatctt ctaaaaattt tatttttttg agtaagttca
     7501 attcttttag agcgctctcc tttgacacac gacatctctt agaaacatct tcttttataa
     7561 aagttatttc cgggcctatt aaaaaaagcc tcattatttt cacacgattt tcacttccaa
     7621 ataattttcc tagtatttcc atcccgttta gaagcagatc gcggtctatc gcaatgactg
     7681 cttttaatta aattaatcaa aatatctgtc tcccaaaatt accgtgctgt ctattcataa
     7741 actgcgagcg cggcttctaa cgggatccat gcaactattc tataaaaata taaaacaaat
     7801 tcaaaataca aaagtcaaaa tgcaaagttt caggtcataa gtcaaaaaat ggtcaaaatt
     7861 ctcgtcctta attcgtaatt cctaattcca caacttactc aaaataaaat cgcccctttt
     7921 gcggggcgat tttattttga gtaagttatt taagtgtaac ctttcctccg gcatcctcta
     7981 tctgcttctt aagagcgtca gcggctgcct tgtccatacc ctccttaagc attgcaggag
     8041 ccttgtctac aagatcctta gcctctttga gaccaagacc caagacctcc ttaacaacct
     8101 tgataacgcc aattttgtta tcacctccgg ctgtcaactc aacgttaaaa gaagacttct
     8161 cagcggcggc ctcaccggcg gctgctgctg gagcggctgc cataacagcg gcctgagcct
     8221 taactccaaa cttctcctcg agaacgtgaa cgagttcgga aagatccaaa acgctcattg
     8281 tctctatctt ctcaacaata tctttgaatt ttgctggtac ttccattgta atttttatta
     8341 atagatttta ttaattctta gattttgcca cctgatcaag agtgcaaaca aaatctctcg
     8401 taaccgcatt caacgttccg acaaatccct gaattggaga ctgcattacg ttcaaaagct
     8461 gtgacaaaag cacttctctt ccaggaatct ttgataacgc tatcatttcc gccgcagaca
     8521 taaacttgcc ctcaagaaca ccacccaata tatagacggc atctttatat ttcttctggg
     8581 cttcagctat agacttagca ggagcgatag gatcctttcc gatagcaaga gcgacctggc
     8641 catccatagg aggaaggttt ccttcgatct tcacatcatt aaaggctctc ttaaataaag
     8701 tctttttggc cacgctaaag cttacgccac catctctcaa agtttttctg agaggagtga
     8761 tgtcggcgat cttgagaccc ttaaaaccaa cgataacggc tgagccggca tctttgagtt
     8821 tgtcgtttaa agactctatt atttcttttt tcttctgtaa tgttattgcc atgatattca
     8881 attacgaatg taaaattaca aattacgaat taaagaaact ttcctttatt cgtaattgat
     8941 aattcgtaat tattaatttt ttattaaaaa tattttttta ataaaaaaat acggctaaaa
     9001 ccgcatgtcg accctcggtg ggaaatagtt ttatgtccag gaacctatct caaaaataac
     9061 cgcttaattt aacaaacaaa gccgccacgc ttgtctgccg aaatgcctaa atttctgcta
     9121 caaatttaaa atattttcgt catagtagct agctaccaat cctcaatatt ttaaattttc
     9181 gctacgaaat ttatgccttt cgcctagcca ttattttaga gataggttcc ggcaacccac
     9241 tttcttcggt tagcaaggta aatataggat aaaacacaaa aatatgcaag tttatcccgt
     9301 tgttcttgaa tcttgtatat atttagcaaa taaaatcccc ccagccgaag ccgggggggt
     9361 agaattcaac caaacacagg ctacttcttg ttctcgagca aggaaatcgc gtaaggacgg
     9421 tagttgcggt aattcgcgat gacttgcgtc aggcgaccct tcttgtcggt gaagagctcg
     9481 acgacataca tgccaatcca gggggaaagg ccgtgtgtgg caaggaactc gtccttatac
     9541 ttgaagcacg gatcgagaag cgcgagctcc tcctggaaaa gcaggtcagc cacctcgaca
     9601 ccgtcttctt ccccgacttc caggccttcc acgaccggct gacctcgatc aacccgcaca
     9661 ttcgcgaccg cttcgaggag caggaggacg agatcgccgc caaccgccgt gccgagagcg
     9721 tccggctgcg ggccgtcgag cgggtgccgg agccggcgtg accgcggtcc gctcccgtgc
     9781 cgacgcgccc cgctcccggg cggccgcgcc cgctc
//
LOCUS       DAOTEX010000029         9626 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3118
            NODE_5010_length_9626_cov_3.898757, whole genome shotgun sequence.
ACCESSION   DAOTEX010000029 DAOTEX010000000
VERSION     DAOTEX010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868142
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 9626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 883
            CDSs (total)                      :: 833
            Genes (coding)                    :: 829
            CDSs (with protein)               :: 829
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9626
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5010_length_9626_cov_3.898757"
                     /isolate="AMR_MDS_3118"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            221..406
                     /locus_tag="P5056_02465"
     CDS             221..406
                     /locus_tag="P5056_02465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007326809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HRY62605.1"
                     /translation="MAKKSVIARAAKTPKFSTRKVSRCFRCGRKRGFMRDFGLCRICF
                     REYANDGKIPGIRKSSW"
     gene            400..837
                     /gene="rpsH"
                     /locus_tag="P5056_02470"
     CDS             400..837
                     /gene="rpsH"
                     /locus_tag="P5056_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020001432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HRY62606.1"
                     /translation="MVGDPIADMITRIKNAAVSGKDSVSVPFSKIKLEIANLLKAEGY
                     LSAVESSGNDKIASARQIKMDLVRVASTNGAVGIKKARISGTERVSKPSRRIYVTAQA
                     IKDGARHATSGKGILVLSTTAGIITGKAAVEKGLGGEALFKIW"
     gene            844..1392
                     /gene="rplF"
                     /locus_tag="P5056_02475"
     CDS             844..1392
                     /gene="rplF"
                     /locus_tag="P5056_02475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HRY62607.1"
                     /translation="MSRIGKQKIAIPAGVTATIALEKGLQVVKIKGPLGELVRDFKPE
                     IKLEIANGEISSTPTAESIFLRSLWGTYMAHIKNMIAGVTKGFKKVLIVEGIGFKVQL
                     AGEKLTFSLGFSHTVEVKLPKGIKATVEKNVITLTSSDKELLGQFAEKIVSLKKPEPY
                     KGKGIKYEGQVIKIKQGKKTVS"
     gene            1406..1756
                     /gene="rplR"
                     /locus_tag="P5056_02480"
     CDS             1406..1756
                     /gene="rplR"
                     /locus_tag="P5056_02480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HRY62608.1"
                     /translation="MKSGNTKETKRARRQKRIRSKVFGTAEKPRLSVYKSNKFTYAQI
                     IDDVSRKTLVSASDMDIKKGGKLARAGEVGKLVAEKAKEKKIKKVVFDRGGFIYTGRV
                     RAVAENARANGLEF"
     gene            1765..2340
                     /locus_tag="P5056_02485"
     CDS             1765..2340
                     /locus_tag="P5056_02485"
                     /inference="COORDINATES: protein motif:HMM:NF012553.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HRY62609.1"
                     /translation="MENNSNPKPQAVTGGAPNGAPARTPFRGGRPGFGGKKFSGKKPD
                     GRPGFVKPDVDYKILNIRRVARITAGGKRFTFAVALVVGDKKGSVGVGTGKAGDTSSA
                     IEKAIKNGKKNMVKVKTTKKMSIPHETAAKFSSSRVVLRPAPGKGLVAGSSARIVLTL
                     AGISDVSSKFHSRTKNPLNNAEATVKALKLL"
     gene            2363..2797
                     /gene="rplO"
                     /locus_tag="P5056_02490"
     CDS             2363..2797
                     /gene="rplO"
                     /locus_tag="P5056_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HRY62610.1"
                     /translation="MQIHQIKRAHPNKAKKIVGRGGKRGTTSGKGTKGQLARSGRKLR
                     PELRDLIKKIPKLRGYRFASVNNPRPATVKLEDVVKVFKDGDVVNLSSLLSNNLISTK
                     KGRVPAVKILGAGDVSKKLNIVGCEISKKAKELIEKAGGSVK"
     gene            2861..4135
                     /gene="secY"
                     /locus_tag="P5056_02495"
     CDS             2861..4135
                     /gene="secY"
                     /locus_tag="P5056_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HRY62611.1"
                     /translation="MSSIWNKIKLALADHDLRARMLFVLFALMIFRLGATIPVPGVDR
                     LALEKFFAGSQVFGLMDIFSGGGLSNLSIFMLGVGPYITASIIMQLMTMIFPKMKEMY
                     QEAGSAGRIKFAQYSRLLSVPLAFFQGLSFLTILGNQGVLLVGSSLFDKLVMALVITA
                     GSTLLMWIGELITEYGIGNGLSLIIFSGIVARIPTTAGELAYKFELSQLPTYIGFLIV
                     AIVIVAGVVVISEAERPIPVTYAKRVRGNKVYGGTSTYLPLRVNQAGVIPIIFALSFL
                     LFPQMFVKFLSGLNIPTLTTISDFLSALLNNQLIYGSAYFLLVVLFTYFYTAVTFDPD
                     SISSNLQKSGAFIPGIRPGVSTTDYLNSVLVRLTLVGSLFLGFIAILPLIMRAVTGDS
                     SLAIGGTALLIVVSVVLELVRQIDAQISMREY"
     gene            4242..4652
                     /locus_tag="P5056_02500"
     CDS             4242..4652
                     /locus_tag="P5056_02500"
                     /inference="COORDINATES: protein motif:HMM:NF012624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HRY62612.1"
                     /translation="MRKNQTFIFIGRSGCGKGTQADLLEDYLKKNNTENSGIFHIITG
                     NFLRALAKENTYTGELIRHVLEHGLLVEEFLPVAMWGKFFADNFKGNEHVITDGMPRK
                     LQEAILLDGAFKFYKREKVVVVFIDLSYDSAVKR"
     gene            4656..4856
                     /locus_tag="P5056_02505"
     CDS             4656..4856
                     /locus_tag="P5056_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62613.1"
                     /translation="MRKRDDDTEEAIRERLKFYDAEVAPVIDYYRNNPDYIFLQIDGE
                     PNIPTIFKDIMSRLEKIWENQK"
     gene            4838..5620
                     /gene="map"
                     /locus_tag="P5056_02510"
     CDS             4838..5620
                     /gene="map"
                     /locus_tag="P5056_02510"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HRY62614.1"
                     /translation="MGESKITIKKPEDIKILREGGKRLSYILGEIAKAVRPGVKQTEL
                     NDIAERLIIEGGDKPAFKGYRPYGAKTAFPAALCVSLNDEVVHGIPANQALSEGDLVS
                     LDIGLEHKGLFTDMAITVPVGMIDSAAKKLIDVTKKSLEIGIKEVKDGAKIGNIGYAI
                     EKFVKQYKFGIVRELAGHGVGYAIHEPPYVPNYGKKNSGLTLKEGMVIAIEPMVNEGG
                     DAIYEAEDEWTVKTEDASRSAHFEKTILVTKNGAEVLTPSPL"
     gene            5769..6248
                     /gene="ndk"
                     /locus_tag="P5056_02515"
     CDS             5769..6248
                     /gene="ndk"
                     /locus_tag="P5056_02515"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008770040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HRY62615.1"
                     /translation="MAKNPEEKTLIIMKPDCVVRGLMGEIIARFERKGLRIIGLKMIE
                     LADVLVEEHYSHHKDKPFFHSLKKFMQSAPVVVMALSGYRAVEAVRLIVGPTMGYKAD
                     AGSIRGDFAMSNQNVVHASDSVEAGEAEVKRFFKKDELFDYNRADYTFVYGPEDRGE"
     gene            7103..8473
                     /locus_tag="P5056_02520"
     CDS             7103..8473
                     /locus_tag="P5056_02520"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRY62616.1"
                     /translation="MNDKLKFNFYGGAGTVTGANFLIEVQGLKILLDCGVMQGETIED
                     RTNRSPFPYNPEEINYLFVSHAHLDHVGLIPRLVREGFRGKIFSTPPTKDITEALLID
                     SLGVLRKEAKAEDKPALYTEEEVENAMKLWETIPYRQGIKLKDDLTITFRDSGHIIGS
                     AMFEIFHAGKKILYTGDLGNSPSPLLNDTEMVTDVDYLILESVYGDRNHASHKESKEA
                     LEDIIEDTMNSGGTLMLPAFSIEKTQEILFEIKEMMIQGRIPLVRVYLDSPLAIKVTD
                     IYRKYVDYLNPEAQKIFSKTKNGEIFSFPQLVQTLATEESVSIKDYHHPKIIIAGSGM
                     STGGRILHHEKNYLGDPRSTLLLTGYQSTRTLGRILQEGARTVKIMGDTVNVNARVVT
                     LSGYSAHKGSDDLLNFVNATSDKLKKVFVVLGEPKSSLFLVQRIRDNLAIDAVAPAYR
                     EFHLLS"
     gene            8634..9338
                     /locus_tag="P5056_02525"
     CDS             8634..9338
                     /locus_tag="P5056_02525"
                     /inference="COORDINATES: protein motif:HMM:NF037785.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62617.1"
                     /translation="MDETKALADNPFGVDKHANLKPKIAVSGSAATEHLANTNAFEAC
                     KELGREIVRQGAICITGATTGVPIWSARGAKEAGGISVGLSPASGPREHVSLYNLPLE
                     FMDLIIYTGFGYVGRDMLMTRTSDAIIIGPGRIGTIHEFTVAFEDQKPIGILEGEWDT
                     DETLKLIIERSHRQTHNPKVVFDSNPKALVEKLIELIKKDKEFEETRVYRTSEYWASG
                     GGQAAQPKDPNIKRVM"
BASE COUNT         2884 a         1755 c         2341 g         2646 t
ORIGIN      
        1 tgttattgtt aatgttctat catgtcgggt gtttccaagc tattgaacca taacgcgtta
       61 tttgacttta gcgcgtttta agtgcctcaa tatatttaat gagccgaaat ttcactatgg
      121 ggtttgacat taaatatgct tcttggtagt atagacccca gtttcaaaag ggtgtttttt
      181 aatagttttt aagcttgttt gagtaattta ttagtcattt atggcaaaga aatcagtaat
      241 agcaagagcc gcgaaaactc ctaagttcag tactaggaaa gtctcgcgat gctttagatg
      301 cggtagaaaa agaggtttta tgagagactt cggtctttgt cgtatttgtt ttagggaata
      361 tgccaatgac ggcaaaattc cgggaataag aaaatcatca tggtaggaga cccaatagca
      421 gatatgataa caagaatcaa gaatgctgcc gtatccggca aggattctgt ttctgttcca
      481 ttttcaaaaa ttaaactaga aatcgccaac cttcttaaag ctgaaggtta tttgagtgca
      541 gttgagagtt ccggtaatga caagattgcc agtgcaagac aaatcaagat ggatctcgtg
      601 cgtgtcgctt ctaccaacgg tgctgttggt attaagaaag cgaggataag cggcactgag
      661 agggtttcca aaccatctag aaggatttat gttacagcgc aggctattaa ggatggcgcc
      721 aggcacgcta cgtccgggaa gggcattttg gttctttcta ctacggcggg gataatcaca
      781 ggaaaggctg ccgtcgagaa aggtcttgga ggtgaagcac tttttaagat ttggtaaaca
      841 aacatgtcta gaataggaaa acaaaaaata gctataccgg ccggagtcac agcgacaatc
      901 gcccttgaaa agggtttaca ggtcgtgaaa ataaagggtc cgcttggtga gctcgtaaga
      961 gattttaagc ctgaaataaa gttggagata gcaaacggag agatttcttc cacgcctaca
     1021 gcagagtcaa ttttcctcag atcgctctgg ggaacatata tggctcatat aaagaacatg
     1081 atcgcgggag ttacaaaagg gtttaagaaa gttttgatag ttgaaggtat cggttttaag
     1141 gtccagctgg ccggagagaa acttacattt agcttgggct tttctcatac tgttgaagta
     1201 aaattaccaa agggcatcaa ggccacggtt gaaaagaacg ttatcacact aacaagctcg
     1261 gacaaagaat tgctcggaca atttgcagaa aagatcgttt ctctaaagaa gcctgagcca
     1321 tataagggta agggtatcaa atatgaggga caggttatca agattaagca aggtaagaaa
     1381 accgtctcct aattaaaata aaacgatgaa atctggaaat acaaaagaaa caaagagagc
     1441 aagaagacaa aaaaggatca gatccaaagt ttttggaaca gctgaaaagc caagactctc
     1501 tgtgtataaa tcaaacaaat tcacttatgc tcagataata gacgatgtct caagaaagac
     1561 attggtttcc gcttctgaca tggatataaa gaagggcgga aagctcgcaa gagccggtga
     1621 ggtaggaaaa ctcgttgccg aaaaagcgaa agaaaagaag attaagaaag tcgttttcga
     1681 caggggaggt ttcatttaca caggaagagt aagagccgta gccgagaacg ctcgagctaa
     1741 tggattggag ttttaaaaaa tactatggaa aataacagca acccaaaacc acaggcagta
     1801 acaggaggtg caccaaacgg agcaccggct agaacacctt tcagaggagg aaggcctgga
     1861 ttcggaggca aaaagttctc aggcaaaaag ccggatggca gaccgggatt tgttaagccg
     1921 gatgttgact acaaaatatt aaatattaga agagtggctc gtatcaccgc cggaggcaag
     1981 agatttacct ttgctgtcgc tttggttgtc ggagacaaga aggggtctgt gggagtaggc
     2041 acaggaaagg ccggagatac atcatccgct atcgagaagg caatcaaaaa cggaaagaag
     2101 aacatggtaa aggtaaagac aacaaagaaa atgtctatac ctcacgaaac agccgcaaag
     2161 ttttcaagct caagagtcgt tttgaggccg gctccaggaa agggattggt cgcaggaagc
     2221 tccgctagaa tcgttttaac attggccggc atctctgacg tatccagcaa gttccactca
     2281 agaacaaaga atcctttgaa caacgccgaa gccacggtaa aggctcttaa attgttatag
     2341 tctaaattct taattcaatt atatgcagat acatcaaata aaaagagctc acccaaataa
     2401 ggcgaaaaag atcgtcggaa gagggggaaa gagagggaca acttctggaa agggcaccaa
     2461 aggacagctg gccagatccg gtagaaagtt gagacctgaa ctcagagatc ttataaagaa
     2521 gatacctaaa ttgagaggct acagattcgc ctcggtaaat aatcctcgac ctgcaacagt
     2581 aaaactggag gacgttgtta aggtattcaa ggacggcgat gtcgtaaatc tttcatcctt
     2641 actatcaaac aatcttattt ctacaaaaaa ggggagagtt cccgccgtaa aaatattggg
     2701 cgcaggcgat gtttcaaaaa agttaaacat tgtcgggtgc gaaatttcaa agaaggcgaa
     2761 agaacttata gaaaaggccg gtggctctgt aaaataaccc acgtcgtgtt ttgttttttg
     2821 tcccttaatt cttgattctt tattcataat tcttatttat atgtcttcta tctggaacaa
     2881 aataaaatta gctctcgcag atcatgatct tagagcaaga atgctatttg tgctgtttgc
     2941 tcttatgata ttcagattag gagcgactat ccctgttccc ggtgtagaca gactggctct
     3001 tgaaaaattc tttgccggaa gccaggtttt tggattgatg gatatctttt ccggaggagg
     3061 tttatcaaat ctctctattt ttatgctggg tgttggccca tacattaccg cttcgatcat
     3121 aatgcagctt atgaccatga tcttcccgaa aatgaaggag atgtaccagg aggcgggttc
     3181 tgcggggcga ataaagttcg ctcagtactc aagacttctt tctgtccctc tagcattttt
     3241 ccagggcctc tctttcctca ctatattggg taatcagggg gttcttttgg ttggatccag
     3301 cctgtttgat aagttggtta tggctttggt tataacggcc ggttccactc ttcttatgtg
     3361 gattggtgag cttatcaccg agtacgggat tgggaatggt ttgtctctca taattttttc
     3421 gggtatcgtg gccagaattc caaccacagc cggtgaactc gcttataagt ttgagttgtc
     3481 acagctccct acatatatag gtttcttgat tgtcgcaata gtaattgttg caggtgttgt
     3541 tgtgattagc gaggcggaaa gaccaatccc tgttacatat gcaaagagag taagaggcaa
     3601 taaggtttat ggggggacat ccacctatct tcctttgagg gttaatcagg ccggagttat
     3661 tcctattatt tttgctcttt ctttcctctt gttcccgcag atgttcgtga agtttttatc
     3721 gggattaaac attccgacac ttacaactat ttctgatttc ttgtctgctc ttttgaataa
     3781 ccagcttatt tacggttcgg catacttttt gcttgttgtt ctttttacct acttctatac
     3841 agcagttact tttgatcctg attctatatc ttcaaaccta cagaagagcg gagcatttat
     3901 tcctgggatt aggccgggcg tatctactac agattatttg aattccgtat tagtaaggct
     3961 tactcttgtc ggttcgttat tcttggggtt catagctatt ctgccgctta ttatgagggc
     4021 ggtcacaggt gattcgtcac tcgccatagg aggcacggcg cttctgatcg tggtatctgt
     4081 tgtgcttgaa ctcgtaagac agattgatgc tcagatctca atgagagaat actaaaaagg
     4141 aattgggaat tacgaattag gaattaagaa ttatggaaaa ccgccgggaa gcctcgctcc
     4201 tcggcggttt cgtttttaat attttgtgct agaataaccg catgaggaaa aaccaaacct
     4261 ttatttttat aggcagatca ggttgcggaa aggggacgca ggcggactta ctggaggatt
     4321 atttaaagaa aaataatact gaaaatagcg ggatttttca tataataacc ggtaattttt
     4381 tgcgagcgct cgcgaaagaa aatacttata cgggtgagct catccgtcac gttttggaac
     4441 atgggctctt ggtggaagaa tttttgccgg tcgcaatgtg gggtaaattt tttgctgata
     4501 attttaaagg aaacgagcac gttattacag atggtatgcc taggaagttg caggaagcga
     4561 tacttctgga cggcgcattt aaattttaca agagagaaaa agttgtcgtc gtatttatag
     4621 atctttctta cgacagcgcc gtaaaaagat agcttttgag aaagagagat gacgatacag
     4681 aggaggcgat acgcgagaga ctgaagtttt atgatgcgga ggttgctccg gttatagatt
     4741 attatagaaa taatcctgac tatattttct tgcagataga tggggagcct aatataccta
     4801 cgatatttaa ggacataatg agcagattgg aaaaaatatg ggagaatcaa aaataacaat
     4861 taaaaagccg gaagatataa agatcttgag agaaggcggg aaacgcctct cttacatatt
     4921 gggtgagatt gcgaaagcgg tgcgccccgg tgttaagcag accgagctca acgacatcgc
     4981 agagagacta ataatagaag gcggagataa gccggctttc aaagggtata ggccctatgg
     5041 ggccaaaaca gcctttcctg cggccctttg tgtgtctttg aatgacgagg tagttcacgg
     5101 gattcccgca aatcaggccc tttctgaggg ggatttggtg agtttggata ttggtttgga
     5161 gcataagggc ctcttcacag atatggccat aactgtaccg gtgggaatga tagacagcgc
     5221 cgccaagaaa cttatagacg ttacaaaaaa atccctagag attggaatca aagaagtgaa
     5281 agacggcgcc aagataggga atatcgggta tgcaattgag aaatttgtta aacaatataa
     5341 atttggaata gtaagggagc ttgccggtca tggcgtcggg tatgccatac acgagcctcc
     5401 atacgttccg aattatggaa agaaaaattc cggcctaact ctgaaagagg ggatggttat
     5461 tgcgatagag ccgatggtga atgagggcgg cgacgctatt tatgaagccg aggatgaatg
     5521 gacggtaaag accgaggatg cctcaagaag cgctcatttt gaaaaaacaa tattagtaac
     5581 aaagaatggg gcggaggttc ttacgccgtc gccgctttag ttgacagcgc gtcgttgcga
     5641 gcgaagcgaa gcctgcttgc gcaggcaggc aacccgggga atatcttata ctctagattg
     5701 cttcgcttcg ctcgcaatga caaaaagaaa ttagtaatta gtaattttta attaataatt
     5761 gaatatccat ggcaaagaat ccagaagaga agacgcttat aataatgaag cccgattgtg
     5821 ttgtcagagg cttaatgggg gagataatcg caagatttga gaggaaggga cttaggatta
     5881 taggtcttaa aatgatagag ctggccgatg ttttggtgga agagcattat tctcatcaca
     5941 aagataagcc tttctttcat tcattaaaaa agtttatgca atctgctccg gtcgttgtaa
     6001 tggcgttgtc gggatatagg gccgttgagg cagtcagatt gattgtcggc ccaacaatgg
     6061 gctacaaggc agacgccggt agcataagag gcgattttgc aatgagcaat cagaacgtcg
     6121 tgcatgcctc agatagcgtg gaagcgggag aggcggaagt aaaaagattt tttaagaagg
     6181 acgaattgtt cgattataat agggctgact atacctttgt atacggacca gaagacaggg
     6241 gagaataatt aaaatttctc ttttggtgca taactttgtc gaagactccg cttgctcctc
     6301 gcagtatatc tacatacagc ttcgtcgcaa tgctcgtctt ctcctcgttc tgcatccaaa
     6361 atataaattt ttagaattgt gaattaggaa ttaagaatta cctgtctgcg cacgcagggc
     6421 aggcaggcga attaaaacca tttgggcgtt ccacgtccaa atggttttgt tttagcgtca
     6481 ttgcgagcgg agcgtggcaa tccagtatat tggccaatct taataagagt gatatccggt
     6541 tcctgccctg tagtaagctc cgctttacta tcggggcccg ccccgtagga agtttctctg
     6601 ataagtttaa gttaaggcag atggagccgt aggcgaattc cgagaggcgt tgtcaaaaaa
     6661 tccacgttag taattcgaac tttcctatcg gggcctgccc tgtagtgagc tttgctctgc
     6721 tacggggtta tccacttttt atgtttttgc agaatttttt tcttgatata attaaatagg
     6781 cagcttgata tgccaactag ctatttatat aagcacggga gccgtttctt ttgttacacc
     6841 gtggtgcaac atcgatggca cccacctgga ttcttctagg attggcatat caaccgccgt
     6901 gaaaagacct gtctccgatg ggtcttttct ttttaccctg ttgggtaaaa ttttatttat
     6961 aaaatttaaa atttctttga gaaaaagaaa tttttaccca acagggtgaa cccaatttcg
     7021 agagattatt ttctatccac accttttgct gtgtggtctt ttgtttttag gatataatat
     7081 ataggtgttt tcatattttt agatgaacga taaattaaaa tttaattttt atggtggggc
     7141 cggcacggtt acgggtgcta attttcttat agaggtgcag ggtttaaaga tacttcttga
     7201 ttgcggcgtt atgcaggggg agacgattga agatagaacg aacagaagtc cttttccgta
     7261 taatcctgag gagataaatt atctctttgt ctctcacgcg cacttagacc atgtcggtct
     7321 tattccgagg ttggtgaggg aggggttccg cggaaaaata ttttcgactc cgccgacaaa
     7381 agatattaca gaggcattgc tcatagacag cttgggggtt ttgcgaaaag aggccaaggc
     7441 ggaagataag ccggctttgt ataccgaaga agaggttgaa aacgccatga agctttggga
     7501 aacgattcct tacagacagg ggataaaatt aaaagacgac ctaaccatta cttttagaga
     7561 ttctgggcac ataatcggtt ccgcgatgtt cgagatattt catgccggaa aaaaaatact
     7621 ttatacggga gatctgggca atagcccatc ccctcttctc aacgataccg agatggttac
     7681 agatgttgac tacttaattt tggaaagtgt ttacggagac aggaaccatg cgtctcacaa
     7741 agagagtaag gaggcgttgg aagatataat agaagatacg atgaattccg gaggaacgct
     7801 tatgttgccc gcattttcta tcgagaaaac acaggaaata ttgtttgaaa ttaaagaaat
     7861 gatgattcag ggcaggatac cattggtaag agtttatctg gattcgccgc tcgctattaa
     7921 ggttacggat atttatagaa agtatgtcga ttatttaaat cctgaggctc aaaaaatttt
     7981 ttctaaaact aaaaatggag agattttttc ctttccgcaa ttagttcaaa cgcttgcaac
     8041 ggaggagtct gtgtctatta aagattatca tcacccgaaa atcatcatag cggggtccgg
     8101 tatgtcgaca ggaggaagaa tactgcacca cgaaaagaat tatctgggag accctaggag
     8161 cactcttctt ttgaccggtt atcagtccac cagaactctc ggcagaatcc ttcaggaggg
     8221 ggctaggact gtgaaaataa tgggagacac ggttaatgta aatgccagag ttgttacctt
     8281 gtcgggctat tctgcgcaca agggttcgga cgacctatta aatttcgtta atgcgacttc
     8341 tgataagctt aaaaaagttt tcgttgtcct aggggaacca aaatcatctc ttttcttggt
     8401 ccaaaggata agagacaatt tggccataga tgccgtagcc cctgcataca gagagttcca
     8461 tttattaagt taattagagg tgcgcgtatt tggtgcataa ctgcgtcaaa aaattcgttt
     8521 actcctcaaa gtatatttat acatattgtc gtaagcctca attttttcct tgttctgcct
     8581 ccaaatacgc gcacctcata attgataaat aattaataat aaataaataa tttatggacg
     8641 aaacaaaagc attggcggat aaccctttcg gtgtagataa gcacgccaac ctaaaaccca
     8701 agatagctgt ttccggttcg gccgcgacag aacatttggc caacacaaat gcttttgagg
     8761 cctgtaagga gctcgggaga gagatagtga gacagggcgc catatgtata acgggtgcaa
     8821 ctaccggtgt gccgatatgg tctgcgagag gtgccaagga ggccggcggt atttctgtcg
     8881 gtctttcacc tgcatccggt ccaagagagc atgtttctct atacaatctg cctcttgaat
     8941 ttatggacct tatcatctac accggattcg gatatgtcgg gcgagatatg ctcatgacta
     9001 ggacatcgga tgctattatt atcggtcccg gcagaatcgg aacgatccat gagtttacgg
     9061 tggcgttcga agaccagaag cctatcggta ttctggaggg ggagtgggat acagacgaaa
     9121 cgctcaagtt aataatagaa agaagccaca gacagactca caatccaaaa gttgtttttg
     9181 acagcaatcc caaggcgctc gtagagaagc tcatagaact tatcaaaaaa gataaagaat
     9241 ttgaggaaac acgtgtgtat aggactagcg aatattgggc gtcaggtgga ggccaggcag
     9301 cacaaccgaa agatccgaat attaaaagag ttatgtaatc taaaattagt tataaagttg
     9361 aaagttataa tgttataaag tctaaaaagt tcgctgttca agcgaacttt tctggttgac
     9421 tgcaaactgt atcggtggta tattttggcg agataaattt gttcttcata tattaattca
     9481 ccgcagtgca tttccataaa aggggatgcc cagacctcga cgaacgtcga gagaaagaag
     9541 ggtgtgtcat gggagaccgt ggtgcgtctg agttcggtaa agacctgggg ttgctgcatg
     9601 aggccgttgt gactgggcga aaggtt
//
LOCUS       DAOTEX010000030         9603 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3118
            NODE_5026_length_9603_cov_4.216799, whole genome shotgun sequence.
ACCESSION   DAOTEX010000030 DAOTEX010000000
VERSION     DAOTEX010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868142
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 9603)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9603)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 883
            CDSs (total)                      :: 833
            Genes (coding)                    :: 829
            CDSs (with protein)               :: 829
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9603
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5026_length_9603_cov_4.216799"
                     /isolate="AMR_MDS_3118"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1006)
                     /locus_tag="P5056_02530"
     CDS             complement(<1..1006)
                     /locus_tag="P5056_02530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006875696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62618.1"
                     /translation="MKDENLSGQGKIRIKGARTHNLKDIDLELPRGKMIAFTGLSGSG
                     KSSLAFDTIFAEGQRRYVESLSSYARQFLKQFQKPDVDEITGLSPAISIDQKSHSANP
                     RSTVATITEIYDYLRVLYARIGIPHCLVCNEPIQKLSTDEIIQFIMEKIESSDRKEEL
                     SRVLIFAPVVRGRKGEYYQMLYDFLGKGFFKARVDGTLYHLREQIVLDKNKKHDIDIM
                     VDEIAVSDFGVNNQNVRDRLAESVPLALSNAEDTIRVIFSYDGGKLLLRDLGKKWEEF
                     AMSAKYTCPNDGFSFIEIEPRLFSFNSPYGACPACHGIGTESLWSDEICKECNGQRLR
                     K"
     gene            1388..1687
                     /locus_tag="P5056_02535"
     CDS             1388..1687
                     /locus_tag="P5056_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62619.1"
                     /translation="MKYPYLASREEGGPYIPIPEDVIKKIYGEVHFDVRMKKNPEDKI
                     PAGVDFYAPRDSDNIDGTTKKVYRAGRHVFWITEEIARQLLPNDILFALSPKMKS"
     gene            1835..2404
                     /locus_tag="P5056_02540"
     CDS             1835..2404
                     /locus_tag="P5056_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62620.1"
                     /translation="MLMLLCGIALYFIGFVILYVGRDASFKRVVEKTTWMPAGYVYQN
                     YEELDKVFYFGEEKVRLKNLPKSKQVPLLPKKLTENMVGKNVLLYNVFPLATCFTSFG
                     TCMMLTPPDNAVVANIFFVTISIVSTACCHDFFIQSVGKGAKNIIGRLINSALEQNVP
                     ELKKESLEYQIALLDKEIEDSGTKTKKIK"
     gene            2584..3129
                     /locus_tag="P5056_02545"
     CDS             2584..3129
                     /locus_tag="P5056_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62621.1"
                     /translation="MPKRYCRECKKETEHGVWARGFRCVYYEKHKRVNFKPETILKGV
                     ILTELGNVNILKDLATLGSIIYDIAYKYHGKKADTQDIINAQIALKELGVEIVETDAC
                     GDDFDWPKENQERLRLSIDKMIDESEQMSPDSLTLTSVDKNGKKESVTIYKVSIMLKV
                     LKLNKRGIAYLKRLRRLGFRF"
     gene            3140..3337
                     /locus_tag="P5056_02550"
     CDS             3140..3337
                     /locus_tag="P5056_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62622.1"
                     /translation="MQRYCRECKKETEHKRRYLGDLYKCTDPKHKDDPTRCPDCGNLL
                     DSESKPSDCTDCYYRKNKDRF"
     gene            3351..3605
                     /locus_tag="P5056_02555"
     CDS             3351..3605
                     /locus_tag="P5056_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62623.1"
                     /translation="MSEKENQVINKIFEVEGNLFFCNDFSDILKDLCFLNDVSVVVEK
                     SGILFVSLKVRLRGRKSSIDGVLREMNNFTWQRKGSVHFA"
     gene            3769..4347
                     /locus_tag="P5056_02560"
     CDS             3769..4347
                     /locus_tag="P5056_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62624.1"
                     /translation="MKFDREIIGLIIGVSVFVLLIIASFSTWSTQTLTVKSVSWERTI
                     NVEYLQTYVEECWGYPPSGGRELSNWLALKSYRVSHTKTDSKGRSHTTHETKWHLKRR
                     CKYEIDRWRHVRTERSHNTDFNPVWPNVVLGNRERSATTSQTYSVKYVGEDQRFYDQT
                     YDESEWRTHKPGYAYRTKLNIFGVILGMEQLQ"
     gene            complement(4399..6426)
                     /gene="uvrB"
                     /locus_tag="P5056_02565"
     CDS             complement(4399..6426)
                     /gene="uvrB"
                     /locus_tag="P5056_02565"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015695207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HRY62625.1"
                     /translation="MFNKKFKIKSGFKPEGDQPEAIKKLVDGIKKGLQHQILLGVTGS
                     GKTFTAANVIEKLQKPVLVIVHNKTLAAQLAEEYRQFFPENAVHYFVSYYDYYQPEAY
                     MPITDTYIEKDAKINEEIDRLRHASTQALLSRRDVIIVASVSCIYGLGNPKEYQEENI
                     RLEKGQDIDRLALLKKLISIHFERTTADLTPGTFRAIGNTIEIMPSSEKNIWSLTLSG
                     GAKQKIEKIHLIDAISRKMMGEEDSVFIFPAKHFLVAQSERDRAIKDIKAELDARLKV
                     LKKEKKILEAERLKRRTNYDLAMIKEVGYCSGIENYSRHLSGRKAGEAPDTLLSYFPK
                     DKGGKPDFLTIIDESHVTVPQIGGMYAGDQSRKKTLVDFGFRLPSAMDNRPLKFNEFS
                     ERVGQIIYTTATPADYEREVSGQIVEQIIRPTGLIDPEIDVRPIMESEGYKGQVFDLI
                     KEAEDTIKRGDRVLVTTLTKKMAEEFSDYLKGKGIKAEYLHSDIKTIERIKILTDFRK
                     GKFDCVVGVNLLREGLDLPEVSLIGILDADKEGFLRSETALIQTIGRAARNVNGKVIL
                     YADKITGSMKRAIDETNRRRGIQLEYNKKHGITPKTILKNISDITDSMETTHRKAVGK
                     LLTIDKELFAKNPKALIKEKERQMSEAVKILDFETAAILRDEIKALKESDL"
     gene            complement(6624..7289)
                     /locus_tag="P5056_02570"
     CDS             complement(6624..7289)
                     /locus_tag="P5056_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62626.1"
                     /translation="MIYALALCAFFAVLYAVFYKFVKLSMWESFVVSVITMTALSIFL
                     MILCAAAHASTAYGYSVAGNEQSVTLAPKAPDEFWFSRGYIVNVPDDKTFEVNVEAAR
                     LDWCDDMITEERFPFTRSDSGQKEMFIMGREGITSYDLAKNEILNRGFRAATLQEALA
                     FIKAYPQRPGTEMLILGQGWTGKGGIWYTRYRVSGSGSSLDAFSLWPAQPWFDVCFLV
                     VKY"
     gene            complement(7306..7479)
                     /locus_tag="P5056_02575"
     CDS             complement(7306..7479)
                     /locus_tag="P5056_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62627.1"
                     /translation="MLWYLAIAVISGLLYWFASLCEDNIFYDKIVEIGMVSFLALLLA
                     YALGNSCIVGFTP"
     gene            complement(7744..8889)
                     /locus_tag="P5056_02580"
     CDS             complement(7744..8889)
                     /locus_tag="P5056_02580"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HRY62628.1"
                     /translation="MTTTEKVVLSKSVLMDKVMKDSPLPPQVGQLVEGKVIGHDKLKL
                     YVDVKPFGTALIYGREYLNARDVIKNIHDGDMVTAKVVEPETEDGYIGLSLKEARQAA
                     TWSDAEEAIKNKTVFELLVKDANKGGLIIDWQNVSGFLPASQLKEEHYPRVTDGNKDR
                     IMEELRKLVGKKIAVTIIGATPKEEKLIFSEKIVGEKDRTELVEKYKVGDVLDGEVTG
                     AVDFGIFVKVESGLEGLVHISELDWSLVENPRKLYNVGDKVRAKVIEIKDGKVSLSIK
                     ALKENPWKAAESKYKKGMKVSGIPIKYNKHGVLVSIEEGVAGLVHISNFGTEEKMKQA
                     LELGKSYEFVMNLFEPKDQKMTLVPADIAEKKAAEALAATPVAAVAA"
     gene            9132..>9603
                     /locus_tag="P5056_02585"
     CDS             9132..>9603
                     /locus_tag="P5056_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62629.1"
                     /translation="MHQDKKINSIALYLLTIGVSFLLGMGVGYDSRPQVELATGINNK
                     ETPAALAQTDFAPFWTTWNLINEKYVNGKLGAEDNGTTTKKKMPTDQEKVYGAIQGLV
                     SSLGDPYSVFMTPEQAESFQTEISGTFEGVGMELGIKKGVITVVAPLEGTPAKKA"
BASE COUNT         2637 a         2346 c         2000 g         2620 t
ORIGIN      
        1 cttttctgag cctctgtccg ttacattcct tacatatctc atcgctccaa agagactccg
       61 tgccgatacc atgacaggcg ggacatgctc catatggaga gttgaatgag aaaagtctcg
      121 gctctatttc tatgaaagaa aatccgtcgt tcggacaagt gtattttgcg gacatcgcaa
      181 actcttccca cttcttgccc aaatctctta gaagaagttt gccaccgtcg tatgaaaaaa
      241 taactcttat ggtgtcctct gcgttagaaa gcgccagggg cacagactct gcaagcctgt
      301 ctcttacgtt ctgattgttt acaccaaaat cgctgacggc tatttcgtca accataatgt
      361 ctatatcgtg cttcttattt ttatctaaaa caatttgttc gcggaggtga taaagagtcc
      421 catcaaccct cgccttgaaa aatcccttac caagaaaatc gtagagcatc tggtagtatt
      481 ctccttttct tcctctcaca acaggggcaa atatgagcac gcgagaaagc tcctcctttc
      541 tgtcggaaga ttctattttc tccattatga attggatgat ctcgtcggtg gaaagttttt
      601 gtatcggctc attgcaaaca agacaatgag gaataccgat cctcgcataa agaacacgga
      661 gataatcgta tatctctgta atagtcgcga cggtcgaacg agggttcgcg gagtgtgact
      721 tctggtcaat agagatggcg ggagaaagcc ctgtgatctc atcaacgtca ggcttctgga
      781 attgtttcaa aaactgcctt gcgtaagacg aaagtgactc aacatagcgc ctctgacctt
      841 cggcaaaaat agtatcaaaa gcaagggatg atttacccga accggaaaga ccggtaaagg
      901 ctatcatttt gccgcgcgga agctccagat ctatatcttt taaattgtgt gtcctggcgc
      961 cctttattcg aatttttcct tgcccgctta aattttcatc tttcattgta ttgtctaatt
     1021 aaaattgtgt ttctccgacc ctgccctatg ttcaaattct gaatattttt taaaaaaatt
     1081 ttggcgggta aatcatttat tcgccctcct aaatttttat tgtccattgt tttgttgtat
     1141 tttaaccctt atttcgacca tattgctttg ttatattacc catcaaatta tgtataaaaa
     1201 ttttggcggg taaatttttc atggcgtttt ttggttcttt agtaaagcga tgcattatac
     1261 aattgatttc aaatatatgc aatgtagaaa gagaatgaag ggtggtgtat gatacttcac
     1321 cataatgacc ttcggtaatc tttatccgaa gtattagcac attaaaaata aaaaggaggt
     1381 gctttttgtg aaatatcctt atcttgcatc cagagaagaa ggcggaccct atatccccat
     1441 ccccgaagac gtgatcaaga agatctacgg cgaggttcat ttcgatgtcc gaatgaaaaa
     1501 gaaccccgag gacaagattc ccgcaggagt ggacttctat gcgccgcgtg acagcgacaa
     1561 catcgatggc acgaccaaga aggtctacag agccggacgt catgtctttt ggattacgga
     1621 agaaatcgcc agacagcttc ttccgaacga catcctcttc gcgctcagcc cgaagatgaa
     1681 atcttaaaca caactcccct tgcgtacccc caccacgtgt gggggtttta tttaccctat
     1741 tgagtaaaat atgtaatata atccatgaac acagtcattt gaaaacaaaa aactaaacgt
     1801 tctactctgc tcgaaagagc caaggaggaa aaccatgctc atgcttctgt gcggaatcgc
     1861 tctctacttc atcggatttg tcattctcta cgtgggccgc gacgcctcct tcaagagagt
     1921 tgtggaaaag acaacttgga tgcccgcggg atatgtatat caaaattacg aagagctgga
     1981 caaagttttt tatttcggcg aagaaaaagt tcgactcaaa aatcttccca agtcgaaaca
     2041 ggtcccactc cttcccaaaa aactcacgga aaacatggtc ggcaaaaacg tgctcctcta
     2101 caatgtcttc cctctcgcca cctgcttcac ctccttcggc acatgcatga tgcttactcc
     2161 gcctgacaat gcggtcgtcg caaacatatt tttcgtcacg atatctatcg tgtcgacagc
     2221 ttgctgccat gatttcttta tccaatcagt gggtaagggt gcaaaaaata tcatcggcag
     2281 actcatcaac tcggcgctcg agcagaacgt gccggagcta aagaaagaat ccctcgaata
     2341 tcagattgcc ctgctcgaca aggagatcga ggactccggc accaagacca aaaagatcaa
     2401 gtagagtccg accccaccac ctggtggggt tttctttttt ccctttctgt gcttatatga
     2461 acacggaaaa aggaccttga aaaaatatac ccgcttcacc cgcctaaatt ttagaccaaa
     2521 attttagtag gcaagaaatt ttctggattt gtgaaaaatt tagaagggca agggaggaac
     2581 aatatgccaa aaagatactg ccgagaatgc aaaaaagaaa ccgagcatgg tgtttgggca
     2641 cgaggattcc ggtgtgttta ttacgagaaa cacaaaagag tcaacttcaa acccgagacg
     2701 atcctcaagg gagtcatcct gactgagctt ggcaacgtga acatcttaaa agatctcgcg
     2761 acccttggct cgattattta cgacatcgcg tataagtatc acggcaaaaa agcagacacg
     2821 caagatatca tcaacgcaca gattgcgctc aaagaactcg gtgtcgaaat cgtcgaaacc
     2881 gatgcttgcg gcgacgattt cgattggccc aaagagaatc aagaaagact gcgtctctct
     2941 atcgataaga tgatagacga gagcgaacaa atgagcccgg acagtctcac tctcacatcc
     3001 gttgataaaa acggcaaaaa agaatcggtg accatttaca aggtttcgat aatgctcaag
     3061 gttctcaaat tgaataagcg cggtatcgcc tatctaaagc gactccgcag gctcggattt
     3121 agattctaag gaggcggcca tgcagagata ctgccgagaa tgcaaaaaag aaaccgagca
     3181 caagcgccga tatctcggag acctctacaa atgcaccgac ccaaagcaca aggacgaccc
     3241 gacgaggtgc ccagactgcg gcaacctgct cgactcggaa tcaaaaccga gcgactgcac
     3301 tgattgctac tatcggaaga ataaagacag gttttaagga gacaaaacat atgagcgaaa
     3361 aagaaaacca agtcatcaac aagattttcg aggttgaagg caatttattc ttttgcaacg
     3421 acttctcgga catactcaag gatctttgct tcctaaacga cgtctcggtc gtggtagaaa
     3481 aatcggggat cctcttcgtc agcctcaagg tgagactgcg gggcagaaaa agcagcatcg
     3541 atggagtatt aagagagatg aacaacttca catggcagag aaaaggtagc gttcattttg
     3601 cttgaacacg ccaaaccacc tggaaacagg tggtttttta ttgttttctg aaacttgact
     3661 ttttagtata tagggtgtat aatataaaga gagttaaggt acaccaaaat taaataatgt
     3721 ggattcccca ccatcgtccg cggcataacc gcaaaggagg gccccgaaat gaaatttgac
     3781 cgggaaatta tcggcctcat catcggtgtc agcgtcttcg tcctcctcat catcgcctcc
     3841 ttctccacgt ggagcaccca gaccctcacg gtcaagtccg tctcttggga gcggaccatc
     3901 aacgtggagt atctccagac ctacgtcgag gaatgttggg ggtatccccc ttccggaggc
     3961 agggagctca gcaattggct ggccctcaaa agttatcgcg taagccacac caagaccgac
     4021 tccaaggggc gtagccacac cacccacgaa accaagtggc acctgaagag acggtgcaag
     4081 tatgagatcg atcgttggag acacgttcgc accgagcgca gtcacaacac cgacttcaac
     4141 ccggtgtggc ccaacgtcgt cctcggcaac cgtgagcgca gtgccaccac ttcccagacc
     4201 tactctgtga agtatgtcgg ggaagaccag aggttctatg accagaccta cgacgaatct
     4261 gaatggcgca cccacaagcc gggttatgcc taccgcacga agctgaacat cttcggcgtc
     4321 atcctcggca tggaacagct ccagtaagag gagcccacaa aaacaaaccc ccatcgaacc
     4381 gatgggggtt ttataatttt ataaatcgct ttcctttaac gcttttatct catcgcgaag
     4441 tattgcggcg gtctcgaaat caagtatctt caccgcttcg ctcatctgtc tttctttctc
     4501 tttaatgagc gccttaggat tttttgcaaa aagctccttg tctatcgtga gcaatttacc
     4561 gacagccttt ctgtgcgttg tttccatcga atcggtaatg tcgctgatat tctttaaaat
     4621 agttttcggt gtgatgccgt gctttttgtt gtattcgagc tgtattcccc ttcttctatt
     4681 cgtctcatct atcgcgcgct tcatagagcc ggttatcttg tcggcataca aaataacctt
     4741 tccgttcaca tttcttgcgg ctcttcctat tgtttgaata agagcggttt cagatctcaa
     4801 aaatccttcc ttgtcggcat caagaattcc aatgagtgat acttccggca agtcgagccc
     4861 ctctcggagc aagttgactc caacgacgca atcgaacttt ccttttctaa aatcggtcaa
     4921 tatttttatt ctctcaatgg tcttaatatc gctatgtaaa tattctgcct taatcccctt
     4981 ccctttcaga taatcactga actcttcggc catctttttt gtaagcgttg ttacgagaac
     5041 gcggtcgccc ctttttattg tatcttccgc ttcttttatg aggtcaaaaa cctggccctt
     5101 gtaaccctcc gattccatta tcggtcgcac atctatttca ggatcgataa gtcccgtagg
     5161 tctgattatt tgttctacaa tctgcccaga cacctctctc tcataatcag caggtgtcgc
     5221 ggttgtatat attatttgtc cgactctctc tgaaaattca ttgaacttga gaggcctgtt
     5281 gtccattgcg gaaggaagac gaaaaccgaa atccaccagc gtcttctttc tggactggtc
     5341 acccgcatac attccgccga tctgcggcac agtcacatga gactcgtcta taatcgtcag
     5401 gaaatccggc ttgccgccct tgtccttcgg gaaatatgaa aggagcgtat cgggggcctc
     5461 gccggctttt cgccccgaaa gatgtctgga gtagttttct ataccactgc agtagccgac
     5521 ctcttttatc atcgcaagat cgtaatttgt tcgtctcttc aatctctcgg cctcgaggat
     5581 ctttttctct tttttcaaaa ctttcaaacg ggcgtccaat tcggccttaa tgtcttttat
     5641 cgccctatcc ctctcgcttt gcgctaccaa aaaatgcttt gccggaaaaa taaagacgga
     5701 atcttcctcc cccatcattt ttctggaaat tgcatcaata agatgtattt tttctatctt
     5761 ctgctttgct cctccggaaa gtgttaagct ccaaatattt ttttcagaag aaggcattat
     5821 ctctatcgtg ttgccgattg ctctaaatgt gcccggcgta aggtcggccg ttgtcctttc
     5881 aaaatgtata gaaataagtt tcttcagaag cgcgagcctg tctatatctt gccctttttc
     5941 tagtcggata ttttcttcct gatactcttt cggatttccc aaaccataaa tacaagatac
     6001 ggaggcgacg ataataacgt cacgcctaga gagaagcgcc tgtgtagaag cgtgcctcaa
     6061 tctatcaatt tcctcgttta tcttcgcatc cttctctata tatgtatctg ttatcggcat
     6121 atacgcctcc ggctgataat agtcgtaata agagacgaaa taatgaacgg cgttctcagg
     6181 gaaaaattgt cgatactcct cggccagctg agcggcaaga gtcttattgt gcacgataac
     6241 caaaacaggc ttttgtaatt tttctataac gttcgcggcc gtgaatgttt tgcccgaacc
     6301 ggtaactcca agaaggatct ggtgttgcag accctttttg attccatcca ccagcttttt
     6361 tatagcctca ggctggtcac cttccggctt aaaaccgctt tttattttga atttcttatt
     6421 aaacacagac tatattttta caactttatt tcaaaatagg caagcccact ccgccgtcat
     6481 tgcgagcgca gcgaagcaat ccatgtctag ccaagatatt tctggattgc ttcgctgcgc
     6541 tcgcaatgac ccagtgcgct acctttctac cgggccaggc gcaaaaaggc ccggggttaa
     6601 ccgggccgct cttgaaactt tatttaatac ttcacgacaa ggaagcagac atcaaaccat
     6661 ggctgcgcgg gccacagaga gaaagcgtcc agcgaactgc cgctccccga gacacgatat
     6721 cgtgtgtacc agatgccacc cttgccggtc cacccctgcc ccaagatgag catttcggtg
     6781 ccgggcctct gaggataggc cttgatgaac gcgagcgcct cttgaagggt cgccgcacga
     6841 aagccgcgat tcaagatctc gttcttcgca aggtcgtatg acgtaatgcc ctcacggccc
     6901 atgatgaaca tttctttctg ccccgaatcg gacctggtga aagggaatcg ctcctcggta
     6961 atcatatcat cgcaccaatc gaggcgcgcc gcttccacgt tcacttcgaa agttttgtcg
     7021 tcggggacat tcacgatgta gccgcggctg aaccagaact catcaggcgc cttgggagca
     7081 agagtcacgc tctgttcgtt ccctgcgacc gagtagccat acgcggtcga agcgtgagcg
     7141 gcggcacaga gaatcatcag gaagatagat agcgccgtca tcgtgatgac ggaaaccaca
     7201 aaactttccc acatgcttaa tttaacgaac ttatagaaga cggcgtacag aacggcgaag
     7261 aaagcacaga gcgccagagc atatatcata ttgcacttcc cttccttacg gcgtaaatcc
     7321 gactatgcac gaattaccga gagcatatgc gagaagaagc gcgagaaagg aaaccattcc
     7381 tatctcgaca atcttgtcat aaaaaatatt atcttcgcac aaagacgcaa accagtagag
     7441 gagccccgag atcacggcaa ttgcgagata ccaaagcatt attatttaca cctctttcta
     7501 atcagcgttt ttgacacacc caaatactgc gcactataaa ccctttcaaa ccgattagtt
     7561 gtgtccactt cctttcaaag agcattattt tatcaaccca taaaatttct tacccatcaa
     7621 aattttagac taaaatttta ttagacgaag cgggtaaatt ttctccgtta aacctatcaa
     7681 ataaaagagt attttgcaat taaaaagacc ccttgtaaaa ggggtctttt tatcaaaaat
     7741 ttattaagcg gcaacggcgg ctacaggtgt agcggcgagc gcttcggcgg ccttcttttc
     7801 ggctatgtcg gcagggacaa gggtcatctt ctgatcctta ggctcaaaca agttcataac
     7861 aaactcgtag ctcttgccga gctcaagcgc ctgcttcatt ttctcttctg ttccaaaatt
     7921 agaaatgtga acaagaccgg caacaccctc ttcgatactt acaagcacac cgtgcttatt
     7981 gtatttgata ggtataccgc ttactttcat tcctttcttg tatttggatt cggcggcctt
     8041 ccatgggttc tccttgagag ccttgataga aagcgaaacc tttccgtcct ttatttcaat
     8101 aaccttggcc cttactttat cgccgacgtt gtatagtttt cttgggttct caacgagtga
     8161 ccaatcaagc tcggaaatgt gaacaagtcc ctcgagaccg ctctcaactt taacgaagat
     8221 accgaaatca accgctcccg taacctctcc gtcaagaaca tcgccaacct tatatttctc
     8281 gacgagctct gttctgtctt tctcgccgac tattttctca gagaagatga gcttctcttc
     8341 ttttggtgta gcgccgatga ttgtaacagc aatcttcttt ccgacaagct tcctaagctc
     8401 ttccattatt ctgtccttgt ttccgtctgt aactctagga tagtgctcct ccttaagctg
     8461 tgaagcggga aggaatcctg aaacgttctg ccagtcaata ataagaccac ccttgttggc
     8521 atccttaacc aagagctcga atactgtttt gttcttgata gcttcttctg cgtcgctcca
     8581 tgtggcggcc tgtctagcct ccttaagaga aagaccgata tagccgtctt ctgtctcagg
     8641 ttcaacgacc ttggcggtaa ccatatcgcc atcgtgaata ttttttatta cgtctctcgc
     8701 gttaagatat tctcttccgt aaataagagc cgtgccgaat ggcttaacgt ctacatataa
     8761 tttaagttta tcgtgaccga taaccttacc ctctacaagc tgtcctacct gtggtggcaa
     8821 tggtgaatct ttcatcacct tatccatgag cacagacttg gacaagacta ctttttctgt
     8881 tgttgtcatt aatttcccaa ttttgctcct ttaaatgggt ttggaggttc ctgtttcgct
     8941 caatttctct cgctaaaaca gtgttactta taatagggcc tagtatatcg aaataaattt
     9001 ttttgtcaaa aaattgaaat taagaattat gaattaagaa attaagaatt tctacaaaat
     9061 aaaaaatggg gataagctcg aaaattttcc gaaaattgaa attaaaataa aactttggca
     9121 taattaatca aatgcatcag gataaaaaaa taaattctat agccctttac ctcctcacaa
     9181 taggggtttc ttttcttttg ggtatggggg tcggatacga ttcacgtccc caagtagagc
     9241 tggcaaccgg aatcaataat aaagaaacac cggccgcgct tgcacaaaca gacttcgctc
     9301 ccttctggac cacatggaat ctcatcaacg aaaaatatgt aaacggcaaa ctgggtgcgg
     9361 aagataacgg cacgacaacg aagaaaaaaa tgccgactga ccaggagaaa gtatacggcg
     9421 ccatccaggg gctagtttca tctcttggcg atccatattc cgtatttatg actcccgagc
     9481 aagcggaaag tttccagaca gagattagcg ggacgtttga aggtgtggga atggagctcg
     9541 gaataaaaaa aggggtgatt actgtcgtag cgccgcttga aggaaccccc gccaaaaaag
     9601 ccg
//
LOCUS       DAOTEX010000031         9285 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3118
            NODE_5264_length_9285_cov_3.815926, whole genome shotgun sequence.
ACCESSION   DAOTEX010000031 DAOTEX010000000
VERSION     DAOTEX010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868142
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 9285)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9285)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 883
            CDSs (total)                      :: 833
            Genes (coding)                    :: 829
            CDSs (with protein)               :: 829
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9285
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5264_length_9285_cov_3.815926"
                     /isolate="AMR_MDS_3118"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            247..1257
                     /locus_tag="P5056_02590"
     CDS             247..1257
                     /locus_tag="P5056_02590"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRY62630.1"
                     /translation="MKIIFFEVNSAETEILSKLVSDIPGVSAEFFEEKLDEQNLGKAK
                     DAEIVSVFVNSELKREIINALPGLKFISTRSTGFDHVDLAAAKEKGVKVSNVPAYGAH
                     TVAEFTFALILSLSRNTYPAYERIKEDDDFDIKGLRGFDLFGKTLGVIGMGRIGKNVI
                     RIARGFGMNVLAYDIKPDVKFAEETCTKCVSLEDVLAGSDIVTIHTFLSPETRHLINK
                     NNIYKMKKGAYLINTARGEIVETEALLEAVASGHLAGAGLDVLEAERQLKDEVHIIKD
                     REAKVRDFKLLYEDHVLIDLPQVLVTPHIAFYTKEAEEEIMKVSVDNIKAFVTGAPQN
                     LV"
     gene            1520..3508
                     /locus_tag="P5056_02595"
     CDS             1520..3508
                     /locus_tag="P5056_02595"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019897329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009678 - pyrophosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HRY62631.1"
                     /translation="MSIFGIAVGASIISLLYGAFLVRSVMSKSAGDDKMKAIQQAIEE
                     GAKAYLARQNKTVAFVGAVVAILLAIWVGKMAAIGFVVGAIASAAAGYIGMVVSVKAN
                     ARVAEEAKKGIKEAFSLGFKGGAVTGFFVVGLALLSLTLFYRYAGDVNALVGLGFGAS
                     LISVFARLGGGIFTKGADVGADLVGKVEAGIPEDDPRNPAVIADNVGDNVGDDAGMAA
                     DLFETYVVSALSVMILGNLFSGGNQAITEFPLLIGAFSIIASIIGSLFVRLTGGIMAA
                     LYKGLAATIVIQLAFFWYLSGNNAFVVFPETIKIFWPLVIGILVTIGMVVITEYYTSK
                     SFSPVQRIAKASTTGHGTNIITGLAVSMQATAMPIILIVVSVYYAYFFAGLYGIALAT
                     MSMLSLAGIIVAIDAFGPITDNAGGIAEMAGLPESVRNVTDPLDAVGNTTKAVTKGYA
                     IASAGLAALVLFASYTQEIALASGAQMAFDLSNVNVIIGLFIGGMMPYLFGSIAMEAV
                     GRSAGSVVEEVRRQFREIKGIMEGTAKPDYAQCVDIVTKAALREMIIPALIPVVVPIL
                     VGYLFGPEALGGLLVGVIVTGIFVGLSMTSGGAAWDNAKKFIEEGNHGGKKSFAHQAA
                     VTGDTVGDPYKDTAGPAINPMIKVANIVALLIAKFLVK"
     gene            complement(3613..4149)
                     /locus_tag="P5056_02600"
     CDS             complement(3613..4149)
                     /locus_tag="P5056_02600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62632.1"
                     /translation="MESKELTPEKVMTRLTGKKKYFNQSRDALNTSQLRAYNRHNRFD
                     EYQRIFVYLKIPKSCSLIKEESPECNCGSCRKQARERFESRYITNASESIYWEEIRGL
                     IHKEICPKFDLHLGSNHDADDILTFNLPNGSATIAYVNSRSFHVDGFGGSHFARSREL
                     ALDLLKLYLKVYGKYCTW"
     gene            4258..4491
                     /locus_tag="P5056_02605"
     CDS             4258..4491
                     /locus_tag="P5056_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62633.1"
                     /translation="MIKEFFLKQALNQALKNAPVPEEQKQMLMNMVMKNPGLFQKIAE
                     EAQVLMKEKGMDQMAAVMEVSKKYESELKGLAA"
     gene            4576..5361
                     /locus_tag="P5056_02610"
     CDS             4576..5361
                     /locus_tag="P5056_02610"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HRY62634.1"
                     /translation="MKAAKNKSPRKPVIAVAQIRYFDTYEPHNVAKIKSFIALAAKAK
                     ADIVCFPESCVHKRDVLRIDHRLLQEICLACKENNIWAIVTDTFEENGKSYNMALLIN
                     RKGKIKGKYRKINLYGDVGKEGRKTFVFKTDFAKIGIAICWDVAFPEIFSRMKKKGAE
                     IIFVPTYWCYEYSAHRKDHKSREKKLLKSLVLTRAFENICFVALASPVSNKPGLVPYS
                     AISCPHFIMKDISGKEGLLVKRINLGEIKKFEELYPNKKVNKK"
     gene            complement(5363..5977)
                     /locus_tag="P5056_02615"
     CDS             complement(5363..5977)
                     /locus_tag="P5056_02615"
                     /inference="COORDINATES: protein motif:HMM:NF020032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HRY62635.1"
                     /translation="MYYTEDKWLQYVRCVKNDIPAPKTILLPTDLVSARQEIVNFNQF
                     PVVLKRIEGCRGDFVDKADTPDEAVEVMKKFWGKGEDRFPIIAQEFVTSNSYRVLTIG
                     GKIIQTALKKSPGWKATGCEAVSFTPFEVDKELEDIIKKLLAVTELELCGYDFARHLD
                     KWVFIEANANPSFDFYREGDEMDQLAEKVLEHLKKLAKAARKSE"
     gene            complement(5941..6237)
                     /locus_tag="P5056_02620"
     CDS             complement(5941..6237)
                     /locus_tag="P5056_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62636.1"
                     /translation="MKKITIGFITNSRLLQDDEAKMFKRVADKMDIDLIFFNSADKMD
                     IKEIEDKVKQCDLVYNDDGEHITNELAKTIEALGGKVVEPSKTCITQKTNGFSM"
     gene            6387..7466
                     /gene="ychF"
                     /locus_tag="P5056_02625"
     CDS             6387..7466
                     /gene="ychF"
                     /locus_tag="P5056_02625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003214682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HRY62637.1"
                     /translation="MMLSIGIVGLPNVGKSTLFNALTRKCVAAENYPFCTIDPSVGVV
                     AVPDERLEKLTKFSNSKKTVPTAIEFVDIAGLVKGAAQGEGLGNKFLSNIREVDAIAE
                     VVRIFSDDKIIHVAGKPEPMRDIEVINLELILADMETVSKRLGNLQREVKRGDKEAVA
                     ENDALSKIQKHLELGKLAQTLPLTDDEKKFVRSMHLLTMKPIVYVLNDKTGGTRVDLA
                     PLMKFFEETGSKWVVVDAAIEHELSALEGDEKDEYRKALELKDDGINDLIKRGYEILN
                     LITFLTTGEDETRAWTTKRGSTAPEAGTAIHTDFKDKFIRADVINWQDLLGAGSYAAA
                     REKGLIRTEGKEYIVKDGDVIEFRI"
     gene            7477..8799
                     /gene="murA"
                     /locus_tag="P5056_02630"
     CDS             7477..8799
                     /gene="murA"
                     /locus_tag="P5056_02630"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HRY62638.1"
                     /translation="MAEIFKVKGLAGKKTLNGVIEVSGAKNAVLQALAFSVLFKDELT
                     IKNVPAIEDVKRMTTLLADLGADVVPKKSGSYSIKFSKGIRSEISEEISKKLRASIVL
                     TGPILARTGEVSFPHPGGCVIGARPIDLFVDGFTKLGAKVDFKDGKYHMKAKDGKLHG
                     AEIFMKNQSVTGTQTLMLAAVLAKGKTIIKNAALEPETKHLADFLNKCGAKIKGAGTS
                     TIEVTGTGLLSAKGKSYITPPDRIEAGSFLILGALAGNNLEIKNCDPSWVEALTENLI
                     SAGVKMQIGKSSIKFSGSGKIPNSDFKGLNIKTHEYPGFPTDVQAPMVVFLTQATGES
                     FVFETIFEGRLNYAEDLRRMGADIKVWDPHRATVKGPTPLRGREVESPDIRAGLAFLL
                     AAILAEGDSVIHNVYYIDRGYGNIEERLRRIGVDIKRECLEVCAPCVN"
     gene            8876..>9285
                     /locus_tag="P5056_02635"
     CDS             8876..>9285
                     /locus_tag="P5056_02635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010251440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HRY62639.1"
                     /translation="MGLFIRKLGIDLGTANTLVYVPGRGIVLNEPSVVAVSQRDNRVI
                     AVGSEAKEMVGKTPDDIIAYRPMKDGVIADYRITEAMLKYYINKSLRKMDIFKPEVMV
                     SVPAGVTSTERRAVIEATIRAGAKMAYVVKEPILA"
BASE COUNT         2549 a         1892 c         2221 g         2623 t
ORIGIN      
        1 atgctggaaa aacgggacat gatggccttc ttggcaaaat cttgtccgac cgcgagcgtg
       61 ttgagacagc ttaagaaacg agactcgctc ctggagagat tcttgaggtc aaggggaatt
      121 ggaacaattt ctttgttttt catacacgat cctcctttgt aaatgttcta ttctttctat
      181 ttttatacta tagtttttgg gaatttaagc aactggatat ataattaggt ggtaaaatta
      241 aaacatatga aaattatatt ttttgaagta aacagcgccg aaacagaaat attgtctaaa
      301 ttggtctcgg acattcccgg tgtttcagcg gaattctttg aagagaagtt ggatgaacaa
      361 aacttgggca aggccaagga tgccgaaatt gtttctgtgt tcgttaattc ggagctgaaa
      421 cgggaaatta tcaatgcttt accgggatta aaatttattt ccacccgctc gacaggtttt
      481 gatcacgtag atttggccgc cgccaaggag aagggggtca aagtttctaa cgttcccgct
      541 tatggcgcgc ataccgttgc tgaattcaca ttcgctctca tcttgtctct ttccagaaat
      601 acatatcctg catacgagag aataaaggag gatgacgatt tcgatattaa ggggctccgc
      661 ggttttgatc ttttcggaaa gacactcggt gtcatcggta tggggcgcat tggtaaaaac
      721 gtaataagga ttgcgcgagg attcggtatg aatgttttgg cttatgacat aaagcccgac
      781 gtgaagtttg cggaagaaac ctgcacaaag tgtgtttcct tggaagatgt gcttgccggt
      841 tctgacatcg ttactattca cactttcttg agtccggaaa caagacatct aataaataag
      901 aacaatattt ataaaatgaa gaagggcgct tatctcataa ataccgcaag gggagagata
      961 gtcgaaacgg aggcactcct tgaggccgtg gcgagcggcc acttggccgg agcggggctt
     1021 gatgtgcttg aggcggagag acagcttaag gatgaggttc atataataaa agatagagag
     1081 gccaaggtta gagatttcaa actgctttac gaggatcatg tgcttataga cctcccgcag
     1141 gtgcttgtta ccccgcacat cgctttttac acaaaagagg cagaggaaga aataatgaaa
     1201 gtgtctgtgg ataatataaa agcgtttgtc accggcgcgc ctcaaaatct cgtttaagat
     1261 attctgaaca aaagctccct aatgcaaggg ggctttttct ttgtctcgtc attgcgagcg
     1321 aagcgaagca acccacgtat caacgctatt aaatctagat tgcttcgcct tgctcgcaat
     1381 gacggtggta ttttaaattt tgaattgtaa ttttgcattt tgacttttac ctttttaatt
     1441 tcagctacaa tatctttaca agatgtcctt tttccaatac aatgtatcgc gtatttataa
     1501 gcattagatt aattaaatta tgtctatttt cggtattgca gttggtgctt ccataatctc
     1561 acttctttat ggagcttttt tggttcgctc tgttatgagc aaatcagcag gagacgataa
     1621 aatgaaggca attcagcagg ctattgagga gggtgctaag gcctacctcg caaggcagaa
     1681 taaaacagtc gcctttgttg gtgctgtcgt cgctattctt ctcgctatct gggtcggcaa
     1741 aatggccgct atcggattcg ttgtcggagc catcgcttca gccgccgccg gttatattgg
     1801 aatggttgtt tctgtaaagg caaacgctcg tgttgctgaa gaggcgaaga aggggatcaa
     1861 ggaagctttc tcgctcggtt ttaagggagg agctgttaca ggatttttcg tagttggtct
     1921 cgctcttctt tcactcacac ttttctacag atacgcaggt gacgtgaacg cccttgtcgg
     1981 tctcggcttc ggtgcttctc ttatctctgt ttttgcaaga cttggaggag gtatatttac
     2041 aaaaggtgcc gacgtgggtg ctgatctcgt tggtaaagta gaggccggaa tccctgagga
     2101 tgatcctagg aaccctgccg ttatcgccga taacgtagga gacaacgtcg gtgatgatgc
     2161 cggtatggcc gctgaccttt tcgaaacata cgtcgtgtcc gctctgtctg ttatgatcct
     2221 cggaaatttg ttctcgggag gaaatcaagc tatcacagag ttcccgcttc ttatcggagc
     2281 tttttcaatt atcgcttcta tcataggttc actctttgta agactcacgg gcggaattat
     2341 ggctgctctc tataaagggc ttgccgcgac aatcgtcatc cagctggctt tcttctggta
     2401 tctttcgggc aataatgctt ttgttgtctt cccggaaacg atcaaaattt tctggccgct
     2461 tgtcataggt attctggtga ctatcggtat ggttgtcatc acagaatact atacatcaaa
     2521 gagcttcagc cctgttcaga gaatcgctaa ggcttctaca accggtcacg gaacgaacat
     2581 aatcacaggt ctcgctgttt ctatgcaggc cacagctatg ccgataattc ttattgttgt
     2641 ttccgtatac tacgcctact tcttcgccgg tctttatggt atcgctcttg ccacaatgag
     2701 catgctctca cttgccggaa taattgtcgc aatcgatgcg ttcggtccta ttacagataa
     2761 cgcgggaggt atcgccgaaa tggccggtct tcctgagtcg gtaagaaatg ttacagaccc
     2821 tctcgatgcc gtcggaaaca caacaaaggc ggttacaaag ggatacgcta tcgcttctgc
     2881 cggtctcgcc gctcttgttc tttttgcttc atatacacag gagattgctc ttgccagcgg
     2941 tgcacagatg gcttttgatt taagcaacgt gaacgttatc atcggtctct ttataggagg
     3001 tatgatgcct tatcttttcg gttctatcgc catggaggcg gtcggaagat cagccggttc
     3061 cgtcgttgag gaggttagaa gacagttcag agagatcaag ggaatcatgg agggaacagc
     3121 caagcctgac tacgcgcagt gcgtagatat cgtcacaaag gccgctctca gagaaatgat
     3181 catccctgct cttatacctg tagtcgttcc tattctcgtc ggatacttgt ttggccctga
     3241 ggccctcgga ggacttctcg tcggtgttat cgttacagga atcttcgtag gactttcaat
     3301 gacatcaggt ggagccgctt gggataatgc caagaaattc attgaagagg gcaatcacgg
     3361 aggaaagaag tcgtttgctc atcaggccgc agtcacagga gatactgtcg gtgatcctta
     3421 taaggataca gccggtcccg ctattaaccc gatgatcaaa gtcgcgaaca ttgtcgcgct
     3481 tttgatagcg aaattcctag ttaaataacg cattcactgt cattcccgcc cccgatcgcg
     3541 gtcgagggta aactccggcg ggaatccaga aataaaaaga cccgccctcg ttatccgagc
     3601 ggcgggtttt tgttaccagg tgcaatattt gccatagacc ttgaggtaaa gcttcagtag
     3661 gtctagcgca agctctctgc ttcttgcgaa gtgtgaacct ccaaaaccgt ccacatggaa
     3721 actgcgactg ttaacatagg cgatggtcgc agatccgttg ggaagattga aggtcaatat
     3781 atcgtccgca tcgtggttcg aaccgagatg gagatcgaat ttagggcata tctctttatg
     3841 aattaatccc cgtatttctt cccaataaat cgattctgag gcgttcgtta tgtatctcga
     3901 ttcaaatctt tctctggcct gctttcggca ggagccacaa ttacattccg gactttcttc
     3961 tttgatgagg ctgcaacttt ttggaatttt taaatacacg aaaatgcgtt gatactcgtc
     4021 gaatctattg tgacgattat atgcccgtaa ttgagatgta tttaatgcgt ctctgctttg
     4081 gttgaaatat tttttcttac cggtaagtct tgtcatgacc ttttcgggtg ttaattcttt
     4141 cgattccatt cgaatccctc cttaagattt tgaaatgtgc tgttcgggtt cagattatat
     4201 ttaatttaat aattagtcaa tattaaattt tatctataga agcgctaata tttaacgatg
     4261 atcaaagaat tttttttaaa acaagcgttg aatcaggctc ttaagaatgc gcccgttccg
     4321 gaggaacaaa agcagatgct tatgaatatg gttatgaaaa atccgggcct ttttcagaag
     4381 atcgccgagg aggctcaggt gcttatgaaa gagaagggga tggatcaaat ggctgccgtg
     4441 atggaggtct ctaagaagta tgaaagtgag ctaaaggggc tcgcggctta ggccaaattt
     4501 agacaaattc tgacatttta tgtcttattt ttggtgccta gacacctcct tttttgagtt
     4561 gtattatagg agcttatgaa agccgcaaaa aacaaatctc ccagaaaacc ggtcattgcc
     4621 gtggctcaaa tacgttattt tgatacgtat gagccccaca atgtggctaa aataaagagt
     4681 tttatagccc ttgccgcaaa ggctaaggcg gatatcgttt gtttccctga atcttgtgtt
     4741 cacaagaggg atgttttaag aatagaccac aggcttcttc aagagatctg tttggcctgc
     4801 aaggagaaca atatttgggc cattgtcacc gatacttttg aggagaatgg aaagagctac
     4861 aatatggctc ttctcataaa ccgaaagggt aaaataaagg gtaaatatag gaaaataaat
     4921 ctctatggag atgtcggtaa agagggccga aagactttcg tttttaaaac agatttcgca
     4981 aaaattggta ttgctatttg ctgggatgtt gccttccctg aaatattttc acgaatgaag
     5041 aagaagggag ccgagataat ctttgttcct acttattggt gttatgagta ttccgctcat
     5101 cgcaaagacc ataagagcag agagaaaaag ttgctaaagt ctcttgtcct gacaagagct
     5161 tttgagaata tttgtttcgt agcgcttgca agtcctgtaa gcaataagcc ggggctagtc
     5221 ccatattcgg ctatatcttg tcctcacttc attatgaaag atataagcgg caaagaaggc
     5281 ctgctcgtaa agagaataaa tttgggtgag ataaaaaagt tcgaagagct ttatccgaac
     5341 aaaaaggtaa ataagaaata aattactccg attttctagc cgctttcgcg agcttcttta
     5401 gatgttcgag gactttttcc gcgagctgat ccatttcatc tccttcgcga tagaagtcga
     5461 atgacggatt ggcgttagcc tcaatgaaga cccatttatc taagtgtctg gcaaagtcat
     5521 atccgcagag ctccagttcg gtcacggcta ggagcttttt gattatatct tcaagctcct
     5581 tatctacttc aaaaggagtg aagctgacag cctcgcaacc ggtagccttc catccggggc
     5641 ttttctttag agcggtttga atgatttttc ctccgattgt aagaactcta taggaatttg
     5701 aagtcacaaa ttcttgggca atgataggga agcggtcttc tcccttcccc cagaattttt
     5761 tcattacctc aaccgcctcg tcgggagtat cagccttgtc tacgaaatct ccccggcatc
     5821 cttctatcct tttcaaaact acggggaatt ggttgaagtt gacgatctcc tgtctcgcgc
     5881 ttacaagatc ggtcggcaat aaaatggttt ttggggccgg tatatcgttt ttcacgcacc
     5941 ttacatactg aagccatttg tcttctgtgt aatacatgtt ttggacggct caacaacttt
     6001 gccgccaagg gcctcaatgg tctttgcgag ttcgtttgtg atatgttcgc cgtcatcatt
     6061 ataaacaagg tcgcactgct taaccttgtc ttcgatctcc tttatgtcca tcttgtcggc
     6121 ggaattaaag aagatgagat cgatgtccat tttgtctgca acgcgcttga acatttttgc
     6181 ttcatcgtcc tgaagcaatc gtgagtttgt tataaaacca atggtaattt ttttcataaa
     6241 atatttctga tatcttcatc atacacgaaa cattttttct tcgccaatat tttttcatta
     6301 aaaatcaaaa ttttgcacat gttttgataa aaaaatattt tataattggc ttgagtttta
     6361 tctaaaaaca aggcatagtt atttgcatca tgttatccat cggtatcgta gggcttccaa
     6421 atgtaggtaa atcgacgctt tttaacgcgc ttaccagaaa gtgcgtggcg gcggagaatt
     6481 atcctttctg cacaatagac ccgtccgtgg gtgtcgttgc tgtgccggat gaaaggcttg
     6541 agaaacttac aaaattttct aattcaaaaa agacggtccc aaccgcaatc gaattcgtgg
     6601 acattgccgg acttgtaaaa ggggccgcac aaggagaggg gcttggaaat aaattccttt
     6661 ctaatattcg cgaagtggat gcgatagccg aagtggtgcg aattttttct gatgacaaga
     6721 ttattcacgt tgcgggcaag ccggagccga tgagggacat tgaggtaata aatcttgagc
     6781 ttattctcgc cgatatggag accgtgtcaa agaggctcgg aaatcttcag agagaggtaa
     6841 aaaggggaga caaagaagct gtcgccgaga atgacgcgtt gtcaaaaatc cagaagcatc
     6901 ttgaactggg caagctagct caaacattgc cgttaactga cgacgaaaag aaatttgtcc
     6961 gatcgatgca cctcctcaca atgaagccga tcgtatatgt attgaacgac aagacaggtg
     7021 ggactcgagt cgacctcgcg ccactcatga aattctttga agagaccggt tctaagtggg
     7081 tcgtcgttga tgccgctatc gagcacgaac tttcggcgct tgaaggcgac gagaaagatg
     7141 aatatagaaa agcgcttgaa cttaaagatg acggaataaa tgatctcata aaaagaggat
     7201 acgaaatttt gaatcttatc acctttctta cgacaggaga ggatgagacg agggcatgga
     7261 ctacaaagag aggttcgacc gcccctgaag ccggaactgc aatacataca gactttaaag
     7321 ataagttcat ccgcgctgac gtaataaatt ggcaggatct tttaggtgcc ggctcgtatg
     7381 ccgccgctcg cgaaaaggga cttataagga cagaaggaaa agaatatatc gtcaaggatg
     7441 gcgacgttat cgagttcagg atataatgcg cttatcatgg ctgaaatatt taaagtaaaa
     7501 ggtttggcgg gtaagaaaac gcttaacggt gttatagagg tctcgggtgc gaaaaatgcg
     7561 gtgcttcagg cgctcgcatt ttctgttttg tttaaagatg agctcacgat aaaaaatgtt
     7621 ccggcgatag aagatgtaaa aagaatgacg acacttcttg ccgatctcgg cgcagatgtt
     7681 gtgcctaaaa aatccggctc atattcaata aaattcagca aaggtattcg ttcggaaatt
     7741 tcagaagaaa tatcaaaaaa attacgcgca tccatagtgc ttaccggccc gatacttgcg
     7801 aggaccggcg aagtttcttt ccctcatcca ggaggctgtg ttatcggtgc aaggccgata
     7861 gacctcttcg ttgacggatt taccaagctt ggcgcgaagg tagatttcaa ggacggcaaa
     7921 tatcacatga aggcaaagga cggcaagctt cacggcgcgg aaatatttat gaaaaatcag
     7981 agcgttactg gcacacagac gcttatgctc gccgccgttc tcgctaaagg aaaaacgatc
     8041 attaaaaatg ccgctctcga accggagacc aagcacctcg ccgattttct aaataaatgc
     8101 ggtgccaaaa taaaaggagc ggggacatct actattgaag tgacgggaac tggactactt
     8161 tctgcaaagg gcaagtcata tatcacgccg cccgacagga tagaggccgg aagtttcctt
     8221 attcttggcg ctcttgccgg taacaatctt gaaataaaga attgcgaccc gtcttgggtg
     8281 gaagcgttaa ccgagaactt gatctctgcg ggagtgaaaa tgcagatcgg gaagagcagt
     8341 ataaaatttt caggcagcgg aaagatacca aatagcgact tcaaaggctt gaatattaaa
     8401 actcacgaat atcccggatt ccccacggac gtccaggcgc cgatggtcgt atttctgacc
     8461 caagcaacag gagaaagttt tgtttttgaa actatttttg aaggacgcct caattacgca
     8521 gaggacctga ggagaatggg agcggatatc aaggtttggg atccgcacag ggcgactgtc
     8581 aaagggccta cgccgcttcg aggaagagaa gtagaaagcc ccgatattcg cgcaggcctc
     8641 gcgttcctct tggccgccat actggctgaa ggagattcgg tgatacacaa tgtctattat
     8701 atagacaggg gatatggaaa catcgaagaa agattacgaa ggattggtgt agatataaag
     8761 agagagtgtc tagaagtgtg cgcaccttgt gttaattagc attctgcatt agattgtatt
     8821 tgtaagtaaa ttatttttta actattttcc agcttctatt ttctactttc taaatatggg
     8881 actattcata agaaaactcg gcatagatct aggaacggct aatacgctcg tgtatgttcc
     8941 cggacgcgga attgttttga acgagccgtc tgttgtggcc gtatctcagc gcgacaatcg
     9001 agtcattgcc gtcgggtctg aggcaaagga aatggtgggc aaaacgccgg atgatattat
     9061 cgcttacaga ccgatgaaag atggagttat cgccgattat agaattacgg aggcgatgct
     9121 caaatactac atcaacaagt cgctcaggaa gatggatatt tttaagccgg aagtaatggt
     9181 gtctgtgccg gccggagtga cctctacaga aagaagagct gttattgagg cgaccataag
     9241 agcaggggca aaaatggctt atgtcgtgaa ggagccgatc cttgc
//
LOCUS       DAOTEX010000032         9071 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3118
            NODE_5439_length_9071_cov_4.200643, whole genome shotgun sequence.
ACCESSION   DAOTEX010000032 DAOTEX010000000
VERSION     DAOTEX010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868142
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 9071)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9071)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 883
            CDSs (total)                      :: 833
            Genes (coding)                    :: 829
            CDSs (with protein)               :: 829
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9071
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5439_length_9071_cov_4.200643"
                     /isolate="AMR_MDS_3118"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1910)
                     /gene="secA"
                     /locus_tag="P5056_02640"
     CDS             complement(<1..1910)
                     /gene="secA"
                     /locus_tag="P5056_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012648158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HRY62640.1"
                     /translation="MYILDKIFGSRNDREIKKMAPIVEKVASFGEGLAKLADEELSSK
                     TKEFRERLSGGETLNDLLPEAFAVVREVSTRVLKQRHYDVQIMGGVVLHEGKIAEMRT
                     GEGKTLVATLPAYLNALAGHVHVVTVNDYLARRDSVWMGQIYSFLGLTTSVINHEASY
                     IYDPKHVEADKERDEVGSFKIVYEFLRPCTRREAYLADIVYGTNHEFGFDYLRDNIAY
                     EERNVVQSFGDFSEKKAYNFAIIDEIDSILIDESRTPLIISAPAGESGELYAAFAKIA
                     ATMNEVEDYVVDEKQKAVTLTDAGITKAEKALGVSNIYADGGIKYVHHLESAVRAKAL
                     FTLDKDYVVKNGEVIIVDEFTGRLQPGRRWSEGLHQAIEAKEGARVQKESKTMATITI
                     QNYFRMYQKLSGMTGTALTSAEEFLSVYNLDTIAIPPNRNSKRTDQDDLVFQTEQGKF
                     KAITRKIKELNEKGQPVLVGTISIEKNELLSKFLEREGIKHSVLNAKNHEKEGEIVAG
                     AGKKGSVVIATNMAGRGVDIKLGGPNASEDEAVEIKALGGLFVLGTERHDARRIDNQL
                     RGRSGRQGDEGETQFFVSLEDALIRIFASDMVKNMMGRLGIPEDEAIQNRLVTKSIEG
                     AQTKIEGINFDT"
     gene            complement(2368..4563)
                     /locus_tag="P5056_02645"
     CDS             complement(2368..4563)
                     /locus_tag="P5056_02645"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRY62641.1"
                     /translation="MNNITIEKLLKNIIIGGLFLIPFLPFIVSGSMFFPFITGKNFAF
                     RIIIEIVFALWLALVIFDKSYLPKKSNLLISVVSFVGIIALADFLGANTFKSFWSNYE
                     RMEGLVSLLHLLTYFVMLITVFNEKIWKYFFNTIFASSIGLFIYGALQLAGKIAINQG
                     GVRLDATFGNTSYFAVHMLFVAFLSGLYFFRGKIGNNFSSWFYGSISLISVFMLMETA
                     TRGTAIGLFMGIVAAAAIVARLGKDTEKKPASFIIGGVVAILLAFLLVKNIPAVKNHP
                     ILGRFAELSVSSLTSQPRYMVWNMAYQGFKERPILGWGQENFNVVFNKYYDPQMYTQE
                     PWFDRAHNVFFDWLIAGGLLGLLSYLSIFLFSILAVWKSDKAKNFTLLDRAVLTGLLG
                     GYFIHNFFVFDNLLSYIMFFAVVAYIYHETSGQPEPEKKQKATPLLEDEFGVYVATSL
                     IIVSFCACMYFVNIKPIMASRSLIQALSSKTVDEAVNNFKKVFSYNTFGTTEGREQLT
                     QRSMSLARSQGVDMMSKQTFFTLATEEMAKQIEKFPGDARYRVFQGALYSSYGLNDNA
                     LKELEEARKLSPKKQMIMFEMVSLLLREGRNKEALDLAKEAFQLEESYQDARIIYAVA
                     LIYNKDDKAADELLSVISDGGVIVDDRLINAYAAVKQFDKVIVIWQKKIEAEPKNAQY
                     HVYLSAAYLADGQREKSVEELKNAAELDPSFADQANAYIRDIRAGKTPQ"
     gene            4965..6215
                     /locus_tag="P5056_02650"
     CDS             4965..6215
                     /locus_tag="P5056_02650"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HRY62642.1"
                     /translation="MPKKTEIIICEWLKWGEKYLKTDLTYFAKGGFWLGSGFGVSMIS
                     TFILSLAFANLLPQESYGIYKYIFSLVGIAGSFYLTGLSTIVSRSVSRGFEGTLFAAV
                     KTSLKWSFIPTIISLSISIFYFYNGNYVLGGAMLIPTLTNPFSNSYSMYSAFLVGKKE
                     FKTTTYYSTISNYFINASLIISLFFTQNPAYIIAVYFIMQMLLVVFFYLLTIKKYSPS
                     KDVEKGAIVYGKHLSVMSVLGTISSNIDKILVFHYLGAAPLAIYSIASAPVDQINALF
                     KQTTHLFMPKISSQNKEEVKKNLPGKSMMLTVIMIVPTIVYILLIPYFYKIFFPKYVS
                     SIIYSQFLALTLLTYPKKPLNIAIVAHGDKKINYFLSVFNPMVLLILKIILLPIWGLT
                     GAIAAEVISPFIYMFVNLYILRRI"
     gene            complement(6221..7972)
                     /locus_tag="P5056_02655"
     CDS             complement(6221..7972)
                     /locus_tag="P5056_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62643.1"
                     /translation="MSMKKSVLVIFGGDFPLKKKEWWSQFNVVVAPKQFEDLIRKYDS
                     QFVDVGELLDSGNIREASELLTQLSLVDTSDGRKISKVVTHEGYELWWIYYGDLMQKF
                     CIPYTQYKRLLEYLKDFSQITLSAPPYAALFKYFFDSYGNKYSILNESQKNILPIGVL
                     IQGIISLPFLLWIKIRGPKLMVWTSDQFDKNGDYDFRMKIIYEELRKKKINFVEFVRS
                     MEPTSIVLKHAIKRKRPVIYSGAIIVIAQYLSSFFNGKEKKEILALSPVMGSDPNQVF
                     LFKVSTGYLSQTLGTIWSIKTLKLFLKFIGIKAAIINDSTSRNFHEGLACKLLNIKTV
                     GIQHGASLKYTLVSDFTPGFNGNKYLSVDKFGLWSEWWREYYIKNSKAYKPEQLYVSG
                     HMRPLEKSAVFSKQNTRALSGGRIKVLFVSEQMSDPKEVLPYLEALVGDNDICLFVKF
                     RFYGDGFEKWLKNDRPDILEKIDKSRILRGSMHEAISKCDVVVGSHSTGVLEASLQLK
                     LPIFFITKKWGDYFELSSFNKNYSFIADNPHNLLNMIKKSDNIPLDVLKDLRCRFFGD
                     PYRNGSKWVVEEVEKLL"
     gene            complement(7985..8842)
                     /locus_tag="P5056_02660"
     CDS             complement(7985..8842)
                     /locus_tag="P5056_02660"
                     /inference="COORDINATES: protein motif:HMM:NF015087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HRY62644.1"
                     /translation="MIKVGNKVLIVAEITTNHLGDINRLKKMVRLAKDAGADLVKIQK
                     RNIETFYSREMLDKEHNSPFGKTWKDFRQGIELSLEDIKEIEAECKKIGIELFVSILD
                     IQSFELLQQFDKKLLKLPSTISSHKDFHAKVAKLYKGEIVISTGYTSLDYEDYIQETF
                     KDNEKIYLLQCTSAYPPPPEDCGISIVRHYHDLSKKNPKIIPGYSSHDIGSLGSMLAV
                     AAGARMIEKHVKIGDTPWMHFQEVALDLENGEFAQFVKDIRQAEIMCGDEIKSVKPSE
                     WHKYSHIPK"
     gene            complement(8835..>9071)
                     /locus_tag="P5056_02665"
     CDS             complement(8835..>9071)
                     /locus_tag="P5056_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013930308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase"
                     /protein_id="HRY62645.1"
                     /translation="DGYEYRIMGCCVYNMYNVIKGSFAAFENPKGAYSWDIIAGLNLA
                     AEHDLVVVVEGKKYNGEFLDPAKKYRFKIKQKND"
BASE COUNT         2742 a         2076 c         1462 g         2791 t
ORIGIN      
        1 ctggtatcaa aatttattcc ctcaatcttt gtctgagccc cctctatgga ttttgttacg
       61 agcctgtttt gaatcgcctc atcctcggga attccgagac gacccatcat gttcttgacc
      121 atatcagaag cgaaaatcct aattagcgcg tcttcgagcg atacaaagaa ctgtgtttct
      181 ccttcatcgc cctgccttcc gcttctacct cgaagctgat tatctattct tcttgcgtca
      241 tgtctctctg tgcccaaaac aaataaccca cccaatgcct ttatctcaac ggcctcatcc
      301 tcgcttgcgt taggccctcc aagcttaata tcaactccac ggcccgccat gtttgtggca
      361 ataacaacac tacccttctt tcctgcaccg gccactatct ctccttcttt ctcatggttc
      421 ttggcgttaa ggacgctgtg ctttatcccc tccctctcta aaaatttgga gagtaattcg
      481 tttttttcaa tagaaatagt tcctactagg accggctggc ccttctcgtt cagctctttt
      541 atcttccttg tgatcgcctt aaacttgccc tgttcggtct gaaaaacgag atcgtcctga
      601 tcggtcctct tgctatttct gttcggcggt attgcaatcg tgtctaaatt ataaacactc
      661 aagaattctt cagcggaagt aagggccgtt ccggtcatac cggacagctt ctgatacatt
      721 ctaaaataat tctgtatcgt gatcgtggcc atggtctttg actctttctg gacacgagca
      781 ccctccttgg cttctatggc ttgatgaagc ccctctgacc atcgccttcc cggctgtagc
      841 ctccccgtga attcatcgac gattattact tcgccgttct ttaccacata atctttgtct
      901 aaagtaaaca gcgccttagc ccttacggct gactctaagt ggtgcacata ctttattccc
      961 ccgtcagcat atatattaga aacacccagg gctttttcag ccttggttat acccgcgtca
     1021 gttaatgtaa ccgccttttg tttctcatct acaacgtagt cctcaacctc attcattgtc
     1081 gccgcgatct tcgcaaaagc ggcataaagt tcacccgatt ctcccgcggg agcggagatg
     1141 ataagaggtg tcctagattc atcaatcaaa atagaatcaa tctcgtctat gatggcaaaa
     1201 ttgtatgctt ttttctctga gaagtcgccg aagctctgaa caacattcct ctcttcataa
     1261 gcgatattat ctctcaaata atcgaagcca aattcgtgat ttgttccata tacgatatcc
     1321 gccagataag cctctcttct cgtgcaaggt cgcaaaaatt cataaacaat cttaaacgac
     1381 ccaacctcat ctctctcttt atccgcctca acatgtttag ggtcatatat ataagaggcc
     1441 tcatgattta ttacggaagt cgtaagaccc aagaaagaat atatctgtcc catccacact
     1501 gaatctcttc gcgcgaggta atcgttaaca gttacaacgt gaacatgtcc ggcgagagcg
     1561 ttaagatagg cgggaagcgt cgcaacgaga gtttttccct ctcccgtcct catctcggcg
     1621 atcttgcctt cgtgcaaaac aactcctccc attatctgaa cgtcgtaatg tctctgttta
     1681 aggacccttg ttgacacctc cctcacaaca gcaaatgcct ccggcaataa gtcgtttaaa
     1741 gtttcgccgc ccgaaagcct ctctctaaac tcttttgttt ttgaggaaag ttcttcgtcg
     1801 gcaagtttag ctagcccttc tccgaaagac gccacttttt ctacgatagg agccattttc
     1861 tttatctccc gatcatttcg gcttccgaat atcttgtcta aaatatacat attcttgcgt
     1921 gttgaatgat taaaagctat ttaagtatat tagcttattt gtaccaaaaa tcatatagac
     1981 aaaacaaaaa acgagtcatg ctcgtttttt acccgcctaa atttcccaac aaataatttt
     2041 cttcttttat tactctaata cgtaaatatc tttcacagag gaccttgtct atatttctgt
     2101 gggaaaattt tggtgggcaa gtatttttta tctacttaaa gttggcggat acaaactcca
     2161 caagtacaaa gaatgtgagc gttccgccaa ccttaactaa acaaaagatc catttaaaat
     2221 tttaacaatt tcttcttaaa aacgcaaaac cccctttcgg gggttagcgc tttgtacttg
     2281 ttttgcgaag tgaagttcaa ataaaatgtt tattattgaa cgacgctcac aatcaaatat
     2341 tatcagaaaa gaatccgtga ggcaagtcta ttgaggcgtt ttcccagccc ttatatccct
     2401 tatatatgcg ttggcctgat ctgcgaaaga aggatccagc tcggccgcat tttttaattc
     2461 ttcgacagac ttctctcttt gcccgtcggc gagatatgcg gctgatagat atacgtggta
     2521 ttgcgcattc tttggctcag cctctatttt cttttgccaa ataacaatga ccttatcaaa
     2581 ctgttttacg gccgcatatg cgttgataag acggtcatcc acgattactc ccccgtcaga
     2641 aattacggac aacaactcat ctgcggcttt atcgtcctta ttgtaaataa gcgccacagc
     2701 ataaattatt cttgcatctt ggtagctctc ctcaagttga aaagcctctt tggccaaatc
     2761 aagtgcctcc ttgttcctgc cttcccttaa aagaagtgag accatctcaa acattatcat
     2821 ttgtttcttc ggagaaagtt ttctggcttc ttctaactcc ttaagggcgt tatcgttaag
     2881 accgtatgac gagtaaagcg ctccttggaa tactctatat ctggcgtctc ccgggaattt
     2941 ctctatttgc ttagccattt cctccgtagc tagagtgaag aatgtttgct ttgacatcat
     3001 atcaaccccc tgagaccttg ctagcgacat tgatctctgc gttagctgtt cccttccttc
     3061 cgtagtgcca aaagtattat acgaaaacac tttcttaaaa ttattaaccg cctcatctac
     3121 cgtcttagaa gaaagggctt gtatcagact tctggaagcc ataatcggtt ttatgtttac
     3181 aaaatacata caggcgcaaa aagaaacaat aatcaatgac gtggccacat aaaccccaaa
     3241 ctcatcctcg agaagaggcg tcgccttttg ctttttttcc ggctccggct ggcccgaagt
     3301 ttcgtgatag atataagcca caaccgcaaa gaacataatg taactcagca agttatcaaa
     3361 cacgaagaaa ttgtgaatga aatatccccc gagaagaccc gtcaaaacag ctcgatccaa
     3421 aagcgtgaaa ttttttgcct tatcgctctt ccacacagcc aaaatagaga acaaaaaaat
     3481 agaaagatac gatagcagcc ccaataaacc tcccgcaatc aaccaatcaa agaaaacatt
     3541 gtgagctcta tcaaaccaag gctcctgagt atacatctgc gggtcgtagt atttattaaa
     3601 tacaacgtta aagttctcct gcccccaacc taatataggc ctctccttga atccctggta
     3661 agccatattc cagaccatgt atcgcggctg actcgttaag ctcgaaacgg acaattcggc
     3721 gaatctacct aatatcggat ggttctttac cgcaggaata ttttttacga gcaagaaagc
     3781 cagcaggatt gccacaaccc ctccgattat aaatgaagcc ggcttcttct ccgtgtcctt
     3841 tcccaatcta gcaacgatgg ccgccgccgc gacgatgccc ataaatagcc ctatcgcagt
     3901 gcccctagtc gcagtctcca taagcataaa gacggagatt agtgaaatgg aaccataaaa
     3961 ccatgaagaa aaattgttgc cgatctttcc cctaaagaaa taaagcccgg aaagaaaggc
     4021 gacaaaaagc atatgaaccg cgaaataaga agtattacca aaagtcgcgt ctaatctaac
     4081 gcctccttgg ttaatggcga tcttgccggc gagctgtagc gcaccataga taaacaggcc
     4141 tatcgaagag gcaaagatgg tgttaaaaaa atacttccaa atcttttcat tgaaaacggt
     4201 aattaacatc acaaaatatg tgagaaggtg aagcagagaa acaagaccct ccattctctc
     4261 atagtttgac caaaaactct taaaagtatt ggcacccaaa aaatcagcca gcgcgattat
     4321 tccgacgaaa gaaacaacgg aaatcaaaag attgcttttc ttcggcaaat aacttttgtc
     4381 aaaaataaca agcgccagcc aaagagcaaa aactatttca ataattatac ggaatgcgaa
     4441 attcttaccc gtaatgaacg gaaagaacat tgaccctgat actataaacg gcaaaaacgg
     4501 aattaaaaat aatccgccga ttataatatt tttcagcaac ttctctattg ttatattatt
     4561 catacagcac aatataacac aaacagccca taatgggtat ttgcaaaaac ctgtgggtgt
     4621 tttgcattgt tcatcgatat ctattaaaac ccccgacctt cttaatgtgt cctctgaatc
     4681 atccacggtc cgtatagtac aaatatcaat aacactttca tcattatcgc ccacaaagag
     4741 aacatctccg aaataaagat attcatctta tccaaccaaa ccgaaaagca acatcgcctt
     4801 tccttagacc atgaacagca gtagactaaa aaaactttcc ctggcacaaa aactccttta
     4861 cgataatctt ctttagtgtt gtggctgtaa ccaaattaaa caataaatag tttcaatgac
     4921 cgtaactcta tcaaaagaca gtaaaatagt ctaaaattat aaaaatgcct aaaaaaactg
     4981 aaataataat atgcgaatgg ctcaaatggg gcgaaaaata tctaaaaaca gacctaacat
     5041 attttgctaa agggggtttt tggcttggtt caggtttcgg agtgtctatg atatccacgt
     5101 tcatactttc tcttgccttc gcaaacctac tcccacagga atcgtatggt atttacaaat
     5161 atatattttc actagttgga attgccggtt cattctatct taccggtctt tctactatag
     5221 tctcacgatc agtatcaaga ggctttgagg ggacactctt tgccgcagtc aaaacaagct
     5281 taaagtggag ctttataccg acaattattt cactttctat atctatcttc tatttctaca
     5341 atggcaatta tgtgctaggt ggagcaatgt taatacccac actcacaaac ccattttcaa
     5401 atagctacag tatgtatagt gcttttcttg tcggtaaaaa agagttcaaa acaactacct
     5461 actacagcac tatctcaaat tatttcataa acgcctctct aataatctca ctttttttta
     5521 ctcaaaatcc cgcttatatt attgctgttt attttataat gcaaatgtta ttggttgtct
     5581 ttttctactt gctcacaatc aaaaaatatt cccccagtaa agacgttgag aagggcgcaa
     5641 tagtatacgg caaacacttg agcgtgatga gcgtcctagg aacgataagc agcaatatag
     5701 acaagatatt agtttttcat tatctgggag cagccccgct tgcgatatat tctatagcat
     5761 cagctcccgt ggatcagatt aatgccctat tcaaacagac aacccactta ttcatgccga
     5821 agatttcttc ccaaaacaaa gaggaagtta aaaagaattt accgggaaag tctatgatgc
     5881 taacagtaat aatgattgtt ccaacaatcg tttatatatt gttaataccg tatttctata
     5941 aaatattttt tccaaaatat gtaagctcta ttatttattc tcaattttta gcattaacgc
     6001 tcctgactta ccccaaaaaa ccgctaaata ttgccatcgt cgcgcacgga gacaaaaaga
     6061 taaattactt tctttctgtt tttaatccaa tggtactctt aattcttaaa ataatattac
     6121 ttcctatttg gggtctaacc ggcgccatcg cagcagaagt aataagccca tttatatata
     6181 tgttcgtaaa tctatatata ctgagaagaa tttaaaaaaa ctacaaaagt ttttccacct
     6241 cttcaaccac ccatttactg ccatttctgt atggatcacc aaaaaaacga catcttaagt
     6301 cttttaatac atccaaaggt atattgtctg attttttaat catatttaac aaattatggg
     6361 ggttatctgc aataaaagaa taatttttat taaagcttga cagctcaaaa taatcccccc
     6421 attttttagt tataaaaaaa ataggcaact tcaactgcaa tgaggcttcc aaaacgcccg
     6481 tactgtgaga gccaaccact acgtcacatt ttgatatggc ctcgtgcata cttcccctga
     6541 gtattctact tttatcaatc ttttccaaga tatctggtct atcgttctta agccattttt
     6601 caaaaccatc gccgtaaaaa cgaaatttta caaacaaaca aatatcatta tctccgacaa
     6661 gagcttccag gtatggcaaa acctctttag gatcagacat ttgttcggaa acaaaaagta
     6721 ctttaatgcg ccctcccgat agagccctag tattttgttt tgaaaagacg gcgctttttt
     6781 ctaagggtct catgtgaccc gaaacataaa gttgttctgg tttataagcc ttgctgtttt
     6841 taatataata ttccctccac cactccgacc aaaggccaaa tttatctacc gaaagatatt
     6901 tatttccatt aaatccaggt gtaaaatcgg aaacaagggt gtacttcaaa gaggcaccat
     6961 gctgaatccc gacagttttt atatttaaca atttgcacgc taggccttca tgaaaattac
     7021 ggctagtaga gtcattgatt atcgcggcct taatcccgat aaatttcaag aataatttca
     7081 atgttttaat agaccagatt gtacccaatg tttgagaaag atatccggtt gagactttaa
     7141 acaaaaaaac ttgattagga tcgctaccca taacgggaga caatgctaat atttcttttt
     7201 tttctttacc attaaaaaaa gacgagagat attgagcgat gacaataatt gctcccgaat
     7261 aaattaccgg tctttttctt ttaatagcgt gttttaaaac gatacttgtc ggttccatgc
     7321 tccttacaaa ttcaacaaaa ttaatttttt tctttctgag ctcttcgtaa attatcttca
     7381 ttctgaaatc gtagtcgcca tttttatcaa attgatcgct tgtccaaacc attaatttcg
     7441 gacctcgtat cttaatccac aaaagaaacg gtagagaaat aataccttgt atcaaaactc
     7501 cgattggcaa aatatttttt tgagattcat ttaaaataga atatttatta ccgtaagaat
     7561 caaaaaaata tttaaaaagt gccgcgtaag gtggcgcaga aagtgttatt tgagaaaagt
     7621 cttttagata ttcaagcaat cttttatatt gagtatacgg tatgcaaaat ttttgcatta
     7681 aatccccata ataaatccac caaagctcgt aaccttcatg ggtaactact tttgaaattt
     7741 tacgaccgtc agaagtgtct acaagagaaa gctgtgttag tagctctgac gcttctctta
     7801 tgttccccga gtctaatagc tccccaacgt caacaaattg ggaatcatat ttcctgatca
     7861 gatcttcaaa ttgttttggc gccacaacaa cattaaattg cgaccaccac tccttctttt
     7921 ttaggggaaa atcaccacca aaaattacta gaactgattt tttcatggac ataacaccat
     7981 attactattt aggaatatga gaatatttat gccactcact tggtttaacg ctttttatct
     8041 catctccaca cataatctcc gcctgtctta tatctttgac aaactgagcg aattcaccat
     8101 tttctaaatc taaggccacc tcctggaaat gcatccacgg agtatctccg atcttaacat
     8161 gtttttcaat cattctagcc ccagccgcca cggcaagcat acttccgaga gacccaatat
     8221 catggctgga ataacctggg attatcttgg gatttttttt gcttaaatca tggtagtgtc
     8281 taacaataga tatgccacag tcttcgggcg gtggtggata tgccgatgta cattgtaata
     8341 aatatatctt ttcattatct ttaaaagttt cctgaatata atcttcataa tccagagatg
     8401 tgtatcctgt tgagataaca atctcaccct tatacaactt tgctacttta gcgtgaaaat
     8461 ctttatggct agagatggtt gacggtaatt taagtagctt cttatcaaat tgctgcaaca
     8521 actcaaatga ttggatatcc aatattgaca caaacaattc aatacctatt tttttacatt
     8581 ctgcctctat ctcttttata tcctcaagag ataattctat tccttgtcta aaatctttcc
     8641 atgtcttacc aaatggagaa ttatgctctt tgtctagcat ttccctagaa taaaaggttt
     8701 ctatgttcct tttctgtatc tttacaagat ctgccccggc atcttttgct agccttacca
     8761 ttttcttaag acggttaata tcaccaagat gattggttgt tatttcagcc acaataagca
     8821 ctttatttcc aactttaatc atttttttgt tttatcttaa aacgatactt tttggcagga
     8881 tctagaaatt caccattgta ttttttaccc tctacaacca caactaaatc gtgttcagcg
     8941 gccaagttta aaccggctat gatatcccat gaataggccc ccttcggatt ttcaaaagcc
     9001 gcgaatgaac cttttataac attatacata ttataaacgc aacatcccat tattctatat
     9061 tcatagccgt c
//
LOCUS       DAOTEX010000033         8816 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3118
            NODE_5636_length_8816_cov_4.687364, whole genome shotgun sequence.
ACCESSION   DAOTEX010000033 DAOTEX010000000
VERSION     DAOTEX010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868142
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 8816)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8816)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 883
            CDSs (total)                      :: 833
            Genes (coding)                    :: 829
            CDSs (with protein)               :: 829
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8816
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5636_length_8816_cov_4.687364"
                     /isolate="AMR_MDS_3118"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="P5056_02670"
     CDS             <1..303
                     /locus_tag="P5056_02670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62646.1"
                     /translation="ATIPHIAILKAIKRLGGELCHFSDVLHLNDCYKDSVDDVHLYLP
                     ICQHEENQEQCPVLEVKKRWCISMEVSGSTMSLDTDWDSYKNIAFRLPAEHPLLNK"
     gene            486..1493
                     /gene="trpS"
                     /locus_tag="P5056_02675"
     CDS             486..1493
                     /gene="trpS"
                     /locus_tag="P5056_02675"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011678752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HRY62647.1"
                     /translation="MEKHTGKQIALTGDRPTGRLHLGHYVGSLKNRVKMQDEFKQFVM
                     VADVQALTDNAENPDKVRNNVIEVALDNLAVGVDPAKTTFFIQSMIPEIAELTVFFLN
                     LVTVARLERNPTIKNEMRQKGFEGNVPAGFLAYPVSQAADILTFKATHVPVGDDQLPV
                     LEQANEISHKFNSLYGETFSKIESIISHAPRLMGIDGGAKMSKSLGNAIFLSDDTETI
                     KKKVMQMYTDPKHIHVNDPGSVEGNVVFDYLTIFDPKQDEVNEFKAEYRKGGLGDTVI
                     KNRLADVLENIISPIREKREYLATKPDYVMNVLLEGTKEARKVAAETMAEVKKVMKIN
                     Y"
     gene            1607..3004
                     /gene="aspS"
                     /locus_tag="P5056_02680"
     CDS             1607..3004
                     /gene="aspS"
                     /locus_tag="P5056_02680"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HRY62648.1"
                     /translation="MERIYIKDLKEHIGQEVKISGWVDVRRDMGKLIFLDFRDESLSK
                     VQMVVLPNNKEAGAVASNVRSEWVLEIVGNVNKRPEKMIKTDVPNGDIEIEVKSIKVL
                     NEATTPPFDISTTGSEVNEESRLKYRYLDLRRERLQKNIRMRHNLISFVREYLGQKGF
                     FEVETPILTKSTPEGARDYVVPSRLYNGKFYALPQSPQQYKQLLMSAGFGKYFQIARC
                     FRDEDTRGDRQPEFTQMDLEMSFVEQEDVMSLIENLIIETVKKVSPEKKIQQVPFPRI
                     KYADAMSKYGSDRPDIREDKNDSNLLAFCWVVDFPFFEKDKQGGWTFTHNPFSMPKPE
                     HEEMLMKGENIGDILTTQYDIVLNGFEIGGGSIRNHHSKGLMKVFEIMGYAADRVREN
                     FGHMLEALDSGTPPHGGIAWGLDRLVMILRNENSIRDVIAFPKTGEGRDLMMDSPSII
                     EKGQLDELGIEIRMK"
     gene            3162..3962
                     /locus_tag="P5056_02685"
     CDS             3162..3962
                     /locus_tag="P5056_02685"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HRY62649.1"
                     /translation="MLDESSQQAVTAEYKVKVGEFEGPLDLLLSLIEKRKLCINDISI
                     AEVTDGYIEYVKGFEEFPMAGSADFLIVASTLLLIKSLSLLPGITLTPEEKDSIEDLE
                     ARLKTYKDIKEKSAFIKECFGKNPIFFGGLKRKADEVIFMPTDEIVLVNMLNSAKALI
                     NNLPKKEKLSEVKIKKVISLEEAIDNLHKKIQQGLRMKFSDFAKTGPNNSPEEAKANK
                     IHVVVSFLAMLELIKRGTLRYNQEVHFADIHLESNDVTTPHYGGSIEN"
     gene            3972..4574
                     /gene="scpB"
                     /locus_tag="P5056_02690"
     CDS             3972..4574
                     /gene="scpB"
                     /locus_tag="P5056_02690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HRY62650.1"
                     /translation="MKLDALIEAILYFRGEPVSKKELAKILEVSKDTLKEALDSLHGK
                     MEGRGIALLETEDDVSLGTAPGASELITAMTKEEMSRDLGKAGLETLTIVAYKGPISR
                     SAIDNIRGVNSSFILRNLLIRGLVERVVDPLDQRAFLYKPTVDLLRFLGISKIEDLPE
                     YDNVVEKLEEPVTEEEAANKEVEDDLAQESPDGGLEDEII"
     gene            4561..5466
                     /locus_tag="P5056_02695"
     CDS             4561..5466
                     /locus_tag="P5056_02695"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62651.1"
                     /translation="MKLFKEKKNMVALGCVLGFIATSAIVPLFYMNISFGSAKTAIAQ
                     KEDLSAFEKLPLKAKSVAIYDTTTGKFIYTKNGNAQLPLASITKVMTSVSALRIAERM
                     DTNRPVKFSGNWWNLADLMKYALVSSSNSGMTSIAEAMSRVEDQNDGSVKNIDFVGEM
                     NSIAKELNLKTTYFLNETGLDVNTTLGGAYGSAKDMARMFAYATDKYPHIFGATKYPE
                     IKIFSKEGANIGSRNTNVDVEKMMALIASKTGTTDLAGGNLVIAFDAGISHPIIISVL
                     GSTPEDRFKDAEELSLATVDYLSSK"
     gene            5708..6673
                     /locus_tag="P5056_02700"
     CDS             5708..6673
                     /locus_tag="P5056_02700"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62652.1"
                     /translation="MWKKKARNEALGGGETPIFNQLHKEKETGTPRMGGIIIWASTLL
                     TILILSLLSDLFPSVATQKLNFLSQNQTWLPLFALVAASLVGLADDLLQVWGGGGYVA
                     GGLALSKRVVFVLIMGFIGAYWFYFKLGMTAIAVPFAGPIELGILFIPFFMLVMLAIF
                     SGGVIDGIDGLAGGIMAVMFSAYGVIAFFQNQIDLATFCAAIVGGTLAFLWFNVPPAR
                     FYMSETGMLGLTVTLSIIAFLTDSVAVLPIIAFPLIATSASVVIQYLSKKYRGKKVFL
                     VAPLHHHFEAIGWPAAKVTMRYWILSIIFAVVGMIVSILGITLTI"
     gene            complement(6861..7181)
                     /locus_tag="P5056_02705"
     CDS             complement(6861..7181)
                     /locus_tag="P5056_02705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62653.1"
                     /translation="MRKREVNILDNQNHDYSKDPAPEGSVKIGPGTYVAIKSSSIHDP
                     SRRTKANEVPEPPTESNYDLRPIARFTFWVVGAIFLLFGKTLGAIAMAFGFAGFAEIC
                     FKKK"
     gene            complement(7315..7527)
                     /locus_tag="P5056_02710"
     CDS             complement(7315..7527)
                     /locus_tag="P5056_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62654.1"
                     /translation="MSKLLNGSARSKNILKFNPDGKVAKLEEIAGRLNREAKEKFPGI
                     DEKDLIVSSTGGKLTLHLKGERGRVL"
     gene            complement(7552..7980)
                     /locus_tag="P5056_02715"
     CDS             complement(7552..7980)
                     /locus_tag="P5056_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62655.1"
                     /translation="MSIRKEDDFTKLLRMSRGIGLKVTAKKLRDSLKNNTKGLKGVIK
                     GKKYARTFFVANIICVDDNRLLTDEEIATLFLDYLYDESSAATALFFNPFLDRIWNIL
                     RKKVDMIPDDKLEDLYFYCKRVASRKTINDIDRKMAKRGL"
     gene            complement(7996..8409)
                     /locus_tag="P5056_02720"
     CDS             complement(7996..8409)
                     /locus_tag="P5056_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62656.1"
                     /translation="MNRRARIYLYTSLVTYLIVIISLIIYLVDNTNGLSLSWIMRGLL
                     CTMTPCAIAIIGLSCAVLQINPKNFDPWLLCLYNLMVPLLALVGSMGFAFEEAQSNRS
                     GADTFVCAILVASFVYGLIEAFIVATIVDEITDHD"
BASE COUNT         2570 a         1741 c         2104 g         2401 t
ORIGIN      
        1 gccacgattc cccatatcgc aattttgaaa gccattaaac gactgggcgg agagctctgc
       61 catttctccg atgttctgca cctcaatgac tgttataaag attcggtgga cgatgtccat
      121 ctgtatttgc caatttgcca acacgaagaa aatcaagagc agtgcccggt gttagaggta
      181 aagaagcgct ggtgtatttc catggaagta tcgggctcga caatgtcgct cgatactgat
      241 tgggattctt ataagaacat tgcttttcgt cttcccgccg aacatccact ccttaacaaa
      301 tagtttctag ttgcagttcc gcggcctata aaaaggccgc tttttattta tgttaaactg
      361 tcgttgcggg cgtagcgaag taatccagga ttagattgcc acgcctcgat taagacatct
      421 cggctcgcaa tgacgaacgt gtgaccatag aaaaaaagac gagtatctgg tatgattgta
      481 aggttatgga aaaacacaca ggaaaacaaa tagcgcttac aggagaccgc ccaacgggca
      541 ggcttcattt ggggcactat gtaggttcgc tcaaaaacag agtgaaaatg caagatgagt
      601 tcaagcaatt cgttatggtt gccgatgttc aggcacttac agacaatgct gaaaatcccg
      661 ataaggtcag gaacaatgtt attgaagttg cattagataa cttggccgtc ggagtagacc
      721 ctgcgaagac gacatttttc atacaatcaa tgattccgga gatagctgag ctcacggtct
      781 ttttcctaaa ccttgtgacg gtcgcgagac ttgaaagaaa tcctactatt aagaacgaaa
      841 tgaggcagaa aggttttgag gggaacgttc ctgcgggatt cctcgcgtat cctgtcagcc
      901 aagccgcgga cattctcact ttcaaggcta ctcatgtgcc tgtcggagac gatcagcttc
      961 cggtgcttga gcaagcaaat gaaatttcac acaagtttaa ttccctttat ggggagactt
     1021 tttcaaaaat agaatcaata atttctcacg cgcctagact tatgggaata gacggcgggg
     1081 cgaagatgag caagtcgcta ggtaacgcca tttttctttc tgatgatact gagacgataa
     1141 aaaagaaagt tatgcagatg tataccgatc cgaagcatat acacgtgaac gatccgggaa
     1201 gtgtagaggg caatgttgtg ttcgattatc ttacaatttt tgatccgaaa caggatgagg
     1261 taaatgagtt caaggccgag tacagaaagg ggggtcttgg tgatacggta ataaagaata
     1321 ggttggcgga tgttctcgag aatataattt ccccaattag agaaaagaga gaatatctcg
     1381 ccacaaagcc ggattatgtt atgaatgttt tgctagaagg aacaaaagag gccagaaagg
     1441 ttgccgcaga aacaatggct gaggtgaaga aggtgatgaa aataaattac tagtaattta
     1501 ttttcatcta gttaaaagtt ataaagtttt aaaaattaaa aaaatagacc tctataacta
     1561 aattaaaaat ttaaacttta taacccgata accttataac taatttatgg aacgaattta
     1621 tattaaagac ttaaaagaac acataggtca ggaagttaaa atatccggtt gggtggatgt
     1681 gaggagagat atgggtaagc tcatatttct agatttcaga gatgaatcgc tttcaaaggt
     1741 tcagatggtc gtgcttccaa acaacaagga ggccggcgcc gtggcaagca atgtcagatc
     1801 agagtgggtt cttgagatcg ttgggaatgt gaacaagagg ccggaaaaga tgatcaaaac
     1861 agatgttccg aacggggata ttgaaattga ggttaaatcc atcaaagttc taaacgaggc
     1921 gacgactccg ccctttgata tttcaactac gggtagtgag gtcaacgaag aatcgaggct
     1981 taaatacaga tacctcgacc ttcgcaggga gaggcttcag aaaaatatca ggatgaggca
     2041 caatcttatt tcttttgtca gagaatattt ggggcagaag ggatttttcg aagtagaaac
     2101 accaatactt acaaaatcaa caccggaagg cgccagagat tatgttgtgc catcgaggct
     2161 ttataacggg aagttttatg cacttcctca gtctcctcag caatacaagc aacttcttat
     2221 gtctgccggg ttcggtaagt attttcagat agctagatgc tttagagatg aagatacgag
     2281 aggagacaga cagccggagt tcacacagat ggaccttgaa atgtcgtttg ttgaacagga
     2341 ggatgtgatg agccttatcg agaatttgat tatcgagacg gttaagaaag tttcgcctga
     2401 aaagaaaata cagcaggttc ctttcccaag gataaaatat gccgacgcaa tgtctaagta
     2461 tggttccgac cgaccggata ttagggagga caaaaacgac tcaaacttgc tcgctttttg
     2521 ctgggttgta gattttccat tctttgagaa agataaacag ggaggttgga cattcacaca
     2581 caatccgttc tctatgccaa agccggagca cgaagaaatg ctcatgaaag gtgaaaatat
     2641 tggcgacata ctcacgactc agtatgacat cgtgttgaat ggttttgaga tcggcggagg
     2701 aagcatcaga aatcaccaca gcaaggggct catgaaagtt tttgaaataa tgggatatgc
     2761 ggcagacaga gttcgcgaga actttggcca catgttagaa gccttagact caggaactcc
     2821 gccacacgga ggtatcgctt ggggtttgga caggcttgtg atgatcttac gaaatgaaaa
     2881 cagcattaga gatgtcatcg ctttcccaaa gactggggag gggcgcgacc ttatgatgga
     2941 ttcgccgtct attatagaga aggggcaatt agacgaattg gggatagaga taaggatgaa
     3001 gtagcggata ttacatattt tcttgtcgcg cgaaatattt tcacgcgtca tttcgacttt
     3061 atggagaaat cacttgaaac ccaataacac gcagaagcga ttcctcggca ggctcggaat
     3121 gacatgcggg attgttactt aaatatgcta aaattagaag tatgttggat gaaagctctc
     3181 agcaggcggt aactgcagaa tacaaagtta aggtaggtga atttgagggg ccactggacc
     3241 ttttgctttc tcttatagaa aaaagaaaac tttgtatcaa tgacatatcg attgccgagg
     3301 tcacagacgg ctacattgaa tacgtaaaag gttttgagga gttcccgatg gcggggagcg
     3361 ccgattttct tattgttgcg tcaacgcttt tgcttataaa atcactctcg cttttgccgg
     3421 gtataactct cactccggaa gagaaagaca gcatagagga ccttgaggcc agactgaaga
     3481 catacaaaga cataaaagaa aaaagcgctt tcataaaaga atgttttggc aagaacccga
     3541 tatttttcgg cgggctaaaa agaaaagccg acgaggtgat atttatgccg acggacgaga
     3601 ttgtcctcgt taatatgttg aattccgcga aggcgctcat aaataatctc ccgaagaaag
     3661 aaaaattatc ggaagtaaaa ataaaaaagg tcataagcct ggaggaggcg atagataatc
     3721 tgcacaagaa gatacagcag gggcttcgta tgaaatttag cgacttcgca aaaacgggcc
     3781 cgaacaattc tcccgaagag gccaaggcga acaagataca cgtcgtcgta agctttctcg
     3841 caatgctcga gcttataaaa agaggcacac ttagatataa tcaagaggtg cactttgcgg
     3901 acatacatct tgagtcaaac gatgttacca cgccgcatta tgggggcagt atagaaaatt
     3961 aatagaacaa aatgaaacta gacgcactta ttgaagccat actatatttt cgcggggagc
     4021 ctgtgtcaaa aaaagaactg gcaaaaatat tggaagtttc caaagacacc ctgaaggagg
     4081 cgctagattc tttgcatgga aaaatggaag gtaggggaat cgctcttctc gaaacagagg
     4141 atgacgtgtc tctcggcact gcgccgggag cctctgagct tataaccgcc atgaccaaag
     4201 aagagatgtc gcgagacctc ggaaaagcgg ggcttgagac gcttacaata gtggcttata
     4261 aaggcccgat aagcagaagc gccatagata atataagagg ggtaaactcc agctttatat
     4321 taaggaatct ccttatcaga ggacttgtcg aaagagttgt cgacccgctt gatcagcgcg
     4381 cttttttata caaaccgacg gttgacttgc ttcgattttt gggaatttca aaaattgaag
     4441 atttgcctga atatgataat gttgtagaga agctagagga gcccgtaacg gaagaggaag
     4501 ccgcgaacaa agaggtagaa gatgatctcg cgcaagaatc tccggatggc ggattggaag
     4561 atgaaattat ttaaagaaaa aaagaatatg gtcgcattgg ggtgcgttct gggctttatc
     4621 gccacgtctg ctatcgtgcc tcttttttac atgaacattt cttttggtag cgcaaaaacg
     4681 gcgattgcgc aaaaagaaga cttgagcgca tttgaaaaat tgcctcttaa ggcgaagtcg
     4741 gtggctatat acgacactac taccggaaag tttatctata cgaagaacgg taacgcgcaa
     4801 ttgccgctag cttccataac aaaagttatg acaagcgtat cggcgctcag gattgcggag
     4861 aggatggata ctaataggcc cgtaaagttt tccggcaact ggtggaatct ggccgatctt
     4921 atgaaatatg ctcttgtatc ttcttcgaat agcgggatga cgagtattgc cgaggcaatg
     4981 tcgcgggtgg aggaccagaa cgacggctcg gtgaaaaaca tagacttcgt gggcgagatg
     5041 aatagtatcg ccaaagagct aaatttgaaa acgacatatt ttttgaacga aaccggcctt
     5101 gatgtgaata cgacactcgg gggcgcttat ggttccgcaa aagatatggc cagaatgttt
     5161 gcatacgcta ccgataaata tcctcacatt tttggcgcga caaaatatcc tgagataaaa
     5221 atattttcta aagagggcgc aaatatcggc tcaaggaata caaacgttga tgtagaaaaa
     5281 atgatggcgc ttatcgcctc taagacgggc acgactgatc tcgccggagg caatctcgta
     5341 atcgcttttg acgcgggcat ttctcatccg ataattattt cggttctcgg gtctactccg
     5401 gaagacagat ttaaggacgc ggaagaattg tctctcgcga cggtggatta tttgtctagc
     5461 aagtagagag taaagagtgg aaggtagaac gagaacgaca cattgtgata tattaaaaga
     5521 agtatttttt agtaaataat taagtggatg tgcagaatag aaaaacttta actattttct
     5581 attttctact ttctaccccc taacaatgat aaatagcgtt gtaaaagttt ttctttcttt
     5641 ttttcttact ttctccgtag gtatttttat aacgccgttt gtcacaaatt atttatataa
     5701 gaataaaatg tggaagaaga aggcgcgaaa cgaggcactg ggtgggggag agacgccgat
     5761 atttaatcag cttcacaaag agaaagaaac cggcactcct agaatgggcg gaataattat
     5821 ttgggccagc acacttttga ccatcttgat attgtctctt ctttccgatt tgttcccgtc
     5881 tgtcgccacg cagaaattaa atttcttgag ccaaaaccag acatggctcc cgctttttgc
     5941 tcttgttgcc gcttctcttg tggggcttgc ggacgacctt ttgcaggttt ggggcggggg
     6001 aggttatgtg gcgggaggat tagcgctttc aaagagagtt gtttttgttc ttatcatggg
     6061 atttatcgga gcttattggt tctattttaa acttggaatg acagctattg cagtgccgtt
     6121 tgcggggccg atagaattag gtatactctt tattcctttc ttcatgcttg tcatgctcgc
     6181 aatattctcg ggaggcgtca tcgacggcat agatggattg gccggaggga taatggctgt
     6241 aatgttttcc gcttatggag tcatcgcttt tttccaaaat cagattgacc tcgctacatt
     6301 ttgcgcggcg atagtagggg gcacactggc gttcctctgg ttcaatgttc cgccggcaag
     6361 attttatatg tcagagacgg ggatgcttgg ccttaccgtc accctctcaa taatcgcatt
     6421 ccttacagac tcggtagcag ttctccctat aatcgctttt cctctcatcg ctacatctgc
     6481 ttctgtcgtg atccaatatc tttcaaaaaa atacagaggc aaaaaagttt ttcttgttgc
     6541 tcctcttcac caccattttg aagcgatcgg atggccagcc gcaaaagtca caatgcgcta
     6601 ctggatacta agcataatat ttgcggttgt agggatgatt gtttcgatac tcggaataac
     6661 gctcactatc taaatttaaa gacacgcttt aaaatcgccc caaagttgtc attcccgccc
     6721 ccaatcaggc cgagggtaaa ctccggtggg aatctaattt ttatctaata tgctctggat
     6781 tcccgccttc gcgggaatga caagcgcgag tttctattat attaaaggaa aaaccgctcc
     6841 gggtggagcg gtttgatatt ttacttcttt ttgaaacaga tttcagcgaa acctgcgaag
     6901 ccgaacgcca tagcaatggc gcccagcgtc tttccgaaga gcaggaagat tgccccgacc
     6961 acccagaatg tgaatctggc aatcggtcgc aagtcgtagt ttgattcggt aggaggctcg
     7021 ggcacttcgt tcgccttggt ccgccgactc ggatcgtgga tagaagaact cttgatggcc
     7081 acgtacgtgc cgggaccgat tttcaccgat ccctcggggg cggggtcctt gctgtagtcg
     7141 tgattttggt tatccaagat attcacctcc ctttttctca tgtcgtttcc gacaagccta
     7201 gagattccac ggttctaaaa ggaatgtcat gatgcgaaac cacaggcaga aggccttttc
     7261 aatcattttg ttctggacgc gtaggatttg taagatggta ttcactctgt ctccttacaa
     7321 cacgcggccg cgttcgccct tgagatgaag ggtgagtttt ccgcccgtcg aggagacaat
     7381 gaggtctttc tcgtcgatgc ccgggaattt ttctttggct tccctgttca gtctgccggc
     7441 gatttcttcc aacttggcga ccttgccatc gggattgaac ttgaggatat ttttactgcg
     7501 agcgcttccg tttagaagtt tactcaaagg agcacctcct tgttgttttt gttagagacc
     7561 acgcttggcc atttttctat cgatatcatt tatcgttttt ctgctagcaa ctctcttaca
     7621 atagaaataa aggtcttcga gtttatcatc aggaatcatg tctacttttt ttcggaggat
     7681 gttccaaatt cgatcgagaa aagggttgaa gaaaagggcg gtcgccgcac ttgattcatc
     7741 atacagataa tccaagaaca gtgtcgctat ctcttcgtca gtaagaagcc ggttatcatc
     7801 aacgcagatt atgttcgcga caaaaaaggt tctcgcgtac tttttaccct tgatcacgcc
     7861 cttgagtccc ttggtattat ttttcaaaga atctctcaat tttttggcgg ttaccttaag
     7921 gccgattcct cgagacattc tgagaagctt cgtgaagtca tcttcttttc ttattgacat
     7981 gctagccccc cttttttaat cgtggtcggt tatttcgtcc actatcgttg cgacaataaa
     8041 ggcctcgatt agtccgtata caaatgaggc cacaagtatc gcgcagacaa aagtatctgc
     8101 gccgcttcgg tttgattgag cctcctcgaa ggcgaagccc atcgagccca caagggccag
     8161 tagaggcacc atgagattat aaagacacag gagccagggg tcgaagtttt ttgggttgat
     8221 ttgcagtacc gcgcaagaga gaccgatgat ggctatggcg cacggcgtca ttgtgcacaa
     8281 aagaccgcgc ataatccacg agagacttag tccgttggtg ttgtccacca ggtagattat
     8341 aagagagatg atgacgatga ggtaagtcac caagcttgta taaagataaa ttctcgctct
     8401 tctgttcatg cgcaatcact ccttattcct gttaatttta aacgatgtca atttactgtt
     8461 tccgggtcta tttttaacaa aaatttgctt gtctgtcaat gtttttgtgt aatgacggta
     8521 gaggcgttat aatataagta tgaatcccgt agtgaaattt tagtttgatt gccgtcgttt
     8581 catctacgca agaaggttta aaatataata ttctgttatc agatattgtt gggcaatata
     8641 gagggcaatc atattaaatc ttcccgcata cggaaaaatg tcaaaataga aatatttagt
     8701 aatgtttttt aaaaataatt gtaagggtcg aaggaagatt taataaacta aaattctact
     8761 acaggatgaa ggtcaaaagc gtagacaaaa tattcctttc cgttcttgtt ctgctc
//
LOCUS       DAOTEX010000034         8663 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3118
            NODE_5755_length_8663_cov_4.318309, whole genome shotgun sequence.
ACCESSION   DAOTEX010000034 DAOTEX010000000
VERSION     DAOTEX010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868142
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 8663)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8663)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 883
            CDSs (total)                      :: 833
            Genes (coding)                    :: 829
            CDSs (with protein)               :: 829
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8663
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5755_length_8663_cov_4.318309"
                     /isolate="AMR_MDS_3118"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1462
                     /gene="uvrA"
                     /locus_tag="P5056_02725"
     CDS             <1..1462
                     /gene="uvrA"
                     /locus_tag="P5056_02725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014463405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRY62657.1"
                     /translation="EINSRLKFMLDVGLNYLTLDRRAGTLSGGEAQRIRLASQLGSHL
                     VGALYVLDEPTIGLHSRDNEQLIKTLKRLRDLGNTIIVVEHDEDTIFASDHLVDIGPG
                     AGVHGGKVVISGDTDELITNRKSHPLGKSLTLDYLRGDKKVAVPEKRRNKEKGALKII
                     NGHANSINGLNLDVPLGRLVCFTGVSGSGKSTLMYDIIHKNLDARLERWRGSKDPINC
                     KNFLGSEYLSRIVLIDQSPIGRTPRSNPATYTGAWTHIRDMFAETTDARVRGWKPSRF
                     SFNVKGGRCETCQGNGMIGVEMHFLPTVYVPCDVCLGKRFEKETLQVKYKGKNIHEVL
                     KTTIEEAAVFFKDIPAISDRLDCLVDVGLGYLELGQSATTLSGGESQRVKIASEFYRP
                     HAERTIYLLDEPTIGLHYEDVGNLVSILQRLVNRGNSVIVIEHNMDVIKSADYIVDMG
                     PEGGENGGKVIATGTPEDVAKNPKSHTGRYLKRVLK"
     gene            1655..1912
                     /locus_tag="P5056_02730"
     CDS             1655..1912
                     /locus_tag="P5056_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62658.1"
                     /translation="MPFAVSAIGALFLLIGTYFKVISSTWVIVYFVGLLFVGIGFYIN
                     RRDDCVPVPVHVHEQEEIDFSKLLDEMQEEFRQLGIKSDKN"
     gene            1936..2079
                     /locus_tag="P5056_02735"
     CDS             1936..2079
                     /locus_tag="P5056_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62659.1"
                     /translation="MPMVSILTGNIPGKESDRPYANVRPPKVEKPKSPNPPETPPGYP
                     KVD"
     gene            2214..3278
                     /locus_tag="P5056_02740"
     CDS             2214..3278
                     /locus_tag="P5056_02740"
                     /inference="COORDINATES: protein motif:HMM:NF022447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2817 domain-containing protein"
                     /protein_id="HRY62660.1"
                     /translation="MLLSNDSARQAQENVWRAALRRGMEFARFSPDLDGLKKDETTDV
                     FYHHGTDGKMIVVISGVHGIEGIPGSLVQQSLFRGWGWPEELSVAVVHAFNSSAFYNM
                     RRTDKNNIDLNRHFSETLDHNPDYAGIRHLVEPEMLTEDSLNIFRNLPKSEMGAFLAT
                     VKGQCEFPEGLFYGGKERGPSYEMLKNICSFLHLQEYKEVVVIDLHTGLGPYKVATLL
                     SPLLSKTSEDAVRTKSWYGDGVNFINVKDNGVVSSVQGDVAPALQRLLPGVKFTGIAL
                     EMGTNTIAESFPALIAENWLYNHPELVNKVNPALVTREMEMFLKSFAPVEDKEWLKAL
                     KMAACKLWCQSIIGLGYELY"
     gene            3614..5764
                     /locus_tag="P5056_02745"
     CDS             3614..5764
                     /locus_tag="P5056_02745"
                     /inference="COORDINATES: protein motif:HMM:NF014516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRY62661.1"
                     /translation="MNLKRLELLGFKSFAKKTVLDFESDVTAIVGPNGSGKSNVAEAF
                     RWALGEQSLKSLRGKRGEDLIFNGSAGHPKLNHASVEVVFNNKERVFAIDFDEVAVSR
                     HIYRDGTNEYFINGTKVRLKDVIELLSAVSLGVSSHHIISQGEADRILNANMFERREM
                     IEDALGLKVFQYKKEESEKKLEKTRENIKEVESLRREIAPHLRFLRKQIEQVKKAEEM
                     RAELISIYKEYFKREEEYLNNFRVVLAKEKGDPDAKLKAVDSEIVRLSAVLGSANTAD
                     PAKVTRLRELDANIYELRSKKNNLEREIGKLDALIEIKSVSEEKGPENRNIRINEKGE
                     RICIYCAQVVPRVVDEVREDEIKQAREKEVSEIRAKRDEVEKMIHSLHESESRVLAEQ
                     DLIRAEIESKKDEFRDSERALYELRAERSNLLSILNSVRAKEEKFNMEEGAFKMEQTE
                     AVVLAGREAANFRDFVIPAETAGEPRVVQEERRRKIERIKIRLEDMGNSGEDVLKEYN
                     EAEERDRFLEKEIGDLTRSAEDLEALMAELSEKVNTEFKKGVERINKQFNEFFMLMFG
                     GGSAELTLVVPQKKKSVEETALGVAMPNEEAEEEGVDINVSLPRKKIKGLQMLSGGER
                     ALTSIALLFAVSQVNPPPFLILDETDAALDESNSKKYGDMLENLSKISQLIVVTHNRE
                     TMSRAGILYGVTMGADGVSRLLSIKFDEAVKIAK"
     gene            6009..6392
                     /locus_tag="P5056_02750"
     CDS             6009..6392
                     /locus_tag="P5056_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62662.1"
                     /translation="MAESNLRDELDRVFRDMETAKRIIANCEWRRRDIFEKLQKECAH
                     KGIIFYCPTRSSSYVPLLMCADCGFGGGEADSVEWVKELLSPVGATLVTEQGMLEACN
                     AIFGEDYIDPTIIHYVEGIEDTGIW"
     gene            6322..6711
                     /locus_tag="P5056_02755"
     CDS             6322..6711
                     /locus_tag="P5056_02755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62663.1"
                     /translation="MAKTILTRPSFITWKALKTPVYGKKEETIMKEDHLNKMKKEILT
                     LNKEISDRIRARDIFRAQIRNSCKHNTNYFYWVMDPPNFEGKSHLGFICCICGKQSLV
                     LAKPGDFPCKNQVPYNDFCAIIRDLTL"
     gene            6725..7225
                     /locus_tag="P5056_02760"
     CDS             6725..7225
                     /locus_tag="P5056_02760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62664.1"
                     /translation="MTENEREKLRQELLERGRQTRDKIVEWEKQHPDETYCGCCHEGR
                     LISCNRIKEIMPGLTSLSLNTLCMSCGRSALGLAAHYTSEWSQTGHSNNSTRVDCVLF
                     DECMFLLQTGHEMPVGDRNAAELVRLECELEAVNNQITHSEKMRSELLASIERIKQEN
                     PAEEKS"
     gene            7247..7534
                     /locus_tag="P5056_02765"
     CDS             7247..7534
                     /locus_tag="P5056_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62665.1"
                     /translation="MKKRKAQIQDPPVLSPFVEQENERIVRVVNKSFFFLTIVSQKSG
                     VTQDALMAVLLHLGIKRMNTDKEYEKKIVELAGKRKDGKRPGPDDTVRKLR"
     gene            7831..8409
                     /locus_tag="P5056_02770"
     CDS             7831..8409
                     /locus_tag="P5056_02770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62666.1"
                     /translation="MDIVVSVNQVLNTMDKVILANCEVPKKVARRKKVHKYEKASYCS
                     DVESKKRHIENSNRKAVLDQISDALDNRTPNPFYTMSEQVPIVTVTPAQIVLAIEPID
                     YLGNVDGDIITHNGMFVSASFGEEVSQSEIVANAPIEVTIGEDGQIASASFGIEPKET
                     PATQQIGQVLIPAGDPNDFRQKQLQDMKPLYC"
BASE COUNT         2425 a         1865 c         2273 g         2100 t
ORIGIN      
        1 agagataaac tcacgactta aatttatgtt ggacgtcggt ttgaattatt tgacgcttga
       61 taggcgcgcc ggcaccttgt cgggaggaga ggctcagaga atcaggcttg cgtctcagct
      121 cggctcacac ctcgtgggag ccctctacgt gctcgatgag cctacaatcg gtcttcactc
      181 acgcgacaac gaacagctca taaaaaccct taagcgatta agggatcttg gcaacacaat
      241 cattgtcgtg gagcatgacg aagacactat cttcgcctca gaccaccttg ttgacatcgg
      301 ccccggggcc ggcgtgcatg gcgggaaagt ggttatctcg ggcgatactg atgagctcat
      361 aacgaataga aaatcgcatc ctttgggcaa gtcgctcact ttggattatt tgagagggga
      421 caagaaggtg gccgttccgg aaaaaagaag gaacaaagaa aagggggcgc tgaaaataat
      481 taacggacac gcgaatagta taaacggact taacctcgat gttccgctcg gaaggcttgt
      541 ttgcttcacg ggtgtttccg gttcgggaaa gtctactctt atgtatgaca tcattcacaa
      601 aaatcttgat gcgagactag agagatggag aggttcaaaa gatcctataa attgtaaaaa
      661 tttcttgggt tcggaatatc tttcgagaat agttcttatc gatcagtctc cgatcggcag
      721 aactcctcgt tcaaatcccg cgacctatac aggtgcatgg actcatataa gagacatgtt
      781 cgcggagacg acagatgcca gggtccgcgg atggaagcca agcagattct ctttcaacgt
      841 aaaaggagga agatgcgaga cctgccaagg aaacggaatg atcggagtcg aaatgcactt
      901 tttgccgacg gtatatgtgc cgtgcgacgt gtgtttgggc aaaagatttg agaaggagac
      961 gctccaggta aaatacaaag gtaaaaatat tcacgaagtt ttgaagacca cgatagaaga
     1021 ggcggccgtc tttttcaaag acataccggc tatatcagat aggctcgact gcttggtgga
     1081 cgtgggtctt ggttatctgg aacttggaca gtctgcgaca acgctttccg gaggagaatc
     1141 acagagggtg aagatcgcct cggagtttta tagaccccac gcagagagaa cgatatatct
     1201 tctcgacgag cctacaatcg gtttgcatta tgaggatgtc ggaaacttgg tcagcatttt
     1261 acagcgcctt gtgaacaggg ggaactcggt tattgttatc gagcacaata tggatgtcat
     1321 caagagcgcc gactatatag tagatatggg gccggaagga ggggagaatg gcggtaaggt
     1381 tatcgcgacc ggcacgccgg aagatgttgc caagaatcca aaatctcaca cgggtagata
     1441 ccttaagagg gtgcttaaat agaagactct tgaccccgta gtgaattttg gcaatgcgct
     1501 tcgcgcgcgg gccattttaa aggcccattg ccaaaattca ctacggggtt gacacgctta
     1561 atctggggtg atataaggat aaacaacata tcgaatagtt ctttgatctt aggcgcttgc
     1621 tcgcttaata gataaaaaag agggggagtc ttgaatgcct ttcgctgtca gcgccattgg
     1681 agcgttgttc ctcttgattg gcacttattt taaggtcatt tcctctacgt gggtcatcgt
     1741 ctattttgtg ggcctcttgt tcgtcggtat cggattttat attaacagga gggacgactg
     1801 cgtccctgtg cccgtccatg ttcacgaaca ggaagagatt gatttcagta agcttcttga
     1861 tgaaatgcaa gaagagttca ggcaactcgg tataaagtcc gacaagaatt agaaagagaa
     1921 agggaggtgc ttattatgcc gatggtcagc attttgaccg ggaacattcc cggcaaggag
     1981 agcgacaggc cctacgccaa cgttcgtccg ccgaaggtgg agaagccgaa gagcccgaat
     2041 ccgccggaga ccccgcccgg ctacccgaag gtcgactagt tcgacacgcg tcccaaaagg
     2101 gcactcgcac gagtgccctt tactttatcc tatatatatt gtagatttgt tattagtttg
     2161 tgtgggcttg ttcttttaaa aaaaggttct aattaaagaa aggatgatgc agaatgttgc
     2221 tttcgaatga ttcagcaagg caggcacagg agaatgtttg gcgcgccgca cttcgtaggg
     2281 gtatggagtt cgcgagattt tctcccgatc tggatggttt gaagaaggat gaaaccacag
     2341 acgtattcta tcatcacggg acggacggca agatgattgt cgttatctcc ggtgtacacg
     2401 gcatcgaggg tatccccggt tcgctggtcc agcagagtct gttccgcggt tggggatggc
     2461 ctgaggagct gtctgtcgct gtcgttcatg cattcaattc gagtgcgttc tacaatatgc
     2521 gtcggacgga caagaataac atcgatctta atcgacattt ttccgaaacg ttagatcata
     2581 atcctgatta cgccggcatc cgccacctcg tcgagcccga aatgctcact gaggattcgc
     2641 ttaatatttt taggaatctt cctaagtcgg agatgggggc gttcctggcc acggtcaagg
     2701 gtcagtgcga attcccggag ggcttattct acggaggtaa ggagcgaggc ccttcttatg
     2761 agatgctgaa aaatatctgt tcttttttgc atcttcagga gtacaaagaa gtcgtcgtta
     2821 tcgatcttca cacggggctc ggtccctaca aggtcgcgac gctcctctct cctcttttgt
     2881 cgaaaacaag cgaggatgca gtccggacga agagctggta tggcgacggc gtcaacttca
     2941 tcaacgtgaa ggataatggc gtcgtgtcga gtgttcaggg agatgtcgcg cccgcattgc
     3001 aacgtcttct tcccggtgtg aagttcacgg gtattgctct cgaaatggga acgaacacga
     3061 ttgccgaaag ttttcccgct cttatcgcgg agaactggct ctataatcac cctgaactcg
     3121 tcaataaggt caatccagcc ctcgtgacgc gcgagatgga aatgtttctc aaatcattcg
     3181 cgcccgtcga ggataaggaa tggttgaagg ctctgaagat ggccgcttgc aaactttggt
     3241 gtcagtcaat tattggcctc ggatacgagc tctattagaa ctgttaaata agtcgcttca
     3301 aggaccccct ttggggtcct tttttaatca aacagaaact tcctgcgggg taaatagtgg
     3361 tatttggttc ttccttttgc aaaaaattgc tataatttac gtatgaacct caagcgtctt
     3421 gaattgcttg gccccagcag gaaagttcga attaggttta tctaagtttg taacattact
     3481 acaatatcat tctgattgtg ctgatattgt agtgatatga aaaagaacgg ttttttattt
     3541 atccagaaac ttcctgcggg gtaaatagtg gtatttggtt cttccttttg caaaaaattg
     3601 ctataattta cgtatgaacc tcaagcgtct tgaattgctt ggctttaagt cttttgcgaa
     3661 aaagacggtt ttggattttg aatctgatgt tacggccatt gtcgggccaa acggttccgg
     3721 aaaatcaaac gtagccgagg ctttccgctg ggcgctcgga gaacagtcgc tcaagtcgct
     3781 ccgaggaaag agaggagaag atcttatttt caacggctct gccggtcatc caaaattgaa
     3841 tcatgcgagc gttgaggttg tgtttaataa taaagaaaga gtttttgcta tcgacttcga
     3901 cgaggttgct gtctccagac atatatatag agacggaacc aacgaatatt tcataaacgg
     3961 caccaaggtt cgcttgaaag atgttattga attgctctcg gcggtgtcgc ttggtgtttc
     4021 gagccatcac ataattagtc agggcgaggc ggacaggata cttaacgcga atatgtttga
     4081 gcgcagggag atgatagaag acgctctcgg tcttaaagtt tttcaataca aaaaagaaga
     4141 atcagaaaag aaattagaaa aaacgcgtga aaatattaag gaggttgaat cgctaaggag
     4201 ggagattgct ccgcatctcc gatttttgcg taagcagata gaacaggtga agaaggccga
     4261 ggaaatgcgc gcggaactta tctctatata taaagaatat tttaaacgcg aagaagaata
     4321 cctgaataat ttccgcgtgg tattggcaaa agaaaaaggg gatcctgatg cgaagctgaa
     4381 ggccgttgat tccgagatcg taagactttc ggctgttttg ggttcagcga atactgccga
     4441 tccggccaag gtgaccagac ttcgtgaact agatgcgaat atttatgaat taagaagcaa
     4501 gaaaaataat cttgagcgtg aaataggaaa actcgatgcc ctcattgaga taaaatcagt
     4561 ttctgaagag aaggggcctg aaaatagaaa tattcgtata aacgaaaaag gcgagagaat
     4621 ctgtatttac tgcgcgcagg ttgtcccacg cgtagtggat gaggtgcgcg aggacgaaat
     4681 aaagcaggcc agagagaagg aggtatcaga gatacgcgca aaaagagacg aagtagaaaa
     4741 aatgatccac tcgcttcacg agagcgagag ccgcgtgctt gcagagcagg atctcataag
     4801 ggctgaaata gaatccaaaa aagatgaatt ccgtgattcc gagagggcgc tttatgagct
     4861 ccgcgcagag aggagtaact tgctctcgat ccttaattcc gttcgcgcca aagaagaaaa
     4921 gttcaatatg gaggagggcg ctttcaaaat ggagcagacg gaggctgtgg tgcttgccgg
     4981 tcgcgaggcc gccaacttcc gcgattttgt gattccggca gagactgccg gtgagcctcg
     5041 cgtagttcaa gaagagaggc gccgcaagat agagaggata aagattaggc tcgaagatat
     5101 gggtaattcg ggcgaggatg ttctcaagga atacaacgag gctgaggagc gcgacagatt
     5161 cctggagaaa gagatcggcg accttacaag aagcgcagaa gacctcgagg cgctcatggc
     5221 ggagctttcg gagaaggtaa atacagagtt caagaagggg gtagagagaa taaacaaaca
     5281 gttcaacgaa ttcttcatgc ttatgtttgg aggagggtcc gccgagctca cgcttgtcgt
     5341 tccccagaag aaaaagtcgg tagaagagac cgctctcgga gtggcaatgc ccaatgagga
     5401 ggcggaagag gagggggtag atataaacgt cagtttgccg agaaaaaaga tcaaggggct
     5461 ccagatgctt tccggggggg agagggccct tacttctatt gcgcttctct ttgctgtgtc
     5521 tcaggtcaat ccgcccccat tccttatcct cgatgaaact gacgccgctc ttgatgaatc
     5581 taattccaag aaatacggag atatgctcga gaatctttca aagatatctc agcttattgt
     5641 cgtcacacac aacagagaga caatgtcacg agccggaatt ttgtatggtg tgacaatggg
     5701 tgccgacggt gtatcgagat tactctcaat caaattcgac gaagcggtca aaattgcgaa
     5761 ataattaagt ttgatttacc cgcaaaaatt ttcgcagaaa atttcggcgg gtaaagcggt
     5821 aaaaatcgct aagtagtttt attttaacta ttttctacat accactttct gctttctatt
     5881 tgggctcagc cctgtactga aaatcgccta gcgatttcta gtacagggtt tacattacta
     5941 cctaaatgta gtagttttct tttaagaacc ttgaaaagtt aataaaaaca atatgaaagg
     6001 agctctgaat ggccgaaagc aatcttcgag acgaactgga tcgggtgttt agggatatgg
     6061 aaactgccaa gagaattatc gccaattgcg aatggcgcag gcgagacatt ttcgaaaagt
     6121 tgcagaaaga atgtgcccac aagggtataa ttttttattg ccccaccagg tcttcttctt
     6181 atgtcccgct tcttatgtgc gccgattgtg gttttggtgg cggagaagcg gactctgtcg
     6241 agtgggtaaa ggagctcctt agtcccgtgg gagcaacgct tgtaacagag caaggtatgc
     6301 ttgaagcgtg taacgccatt tttggcgaag actatattga cccgaccatc attcattacg
     6361 tggaaggcat tgaagacacc ggtatatggt aagaaggagg aaacgataat gaaagaagat
     6421 catcttaata aaatgaagaa agaaattctg accttgaata aagagatcag tgatcggata
     6481 agggcgcggg atatcttccg agctcaaatt cgcaattcct gtaagcacaa cacgaactac
     6541 ttctattggg tcatggatcc tccgaatttt gagggcaagt cgcacctcgg ctttatttgc
     6601 tgcatttgcg gcaagcagag ccttgtgctc gctaagcccg gagacttccc gtgcaagaat
     6661 caggttcctt ataatgactt ttgtgcgatc atccgtgacc tcacattata aggagggtta
     6721 aattatgact gaaaacgaac gcgagaaact tcgccaggaa ttgctcgaga ggggaagaca
     6781 gacgcgtgat aagatcgtcg aatgggaaaa acaacatccc gacgaaactt attgcggttg
     6841 ttgtcatgaa ggaaggctta tctcctgtaa tcggatcaaa gaaattatgc ccgggcttac
     6901 ttctcttagt cttaataccc tatgtatgtc ctgtgggcgc tctgcgttag gactggcggc
     6961 tcactatact tccgaatgga gtcaaacggg acacagcaac aacagcacaa gagttgactg
     7021 cgttttgttt gacgagtgca tgttcttgct ccagaccgga cacgaaatgc ccgtcggaga
     7081 tcgtaatgcg gcggagctgg ttcgcctcga gtgtgagctt gaggctgtga ataaccagat
     7141 tacccattcc gagaagatgc ggagcgaact gctcgcttct atcgagagaa tcaagcagga
     7201 gaatcccgcg gaggaaaaat catgatcgaa gaaagtggag cctgagatga agaaaagaaa
     7261 ggcccagatc caagacccac ctgtcctcag tccttttgtg gaacaagaaa acgagaggat
     7321 cgtccgcgtt gtaaataaga gtttcttttt tctaacgatc gtatcacaaa aatcgggtgt
     7381 gactcaagac gccttgatgg cggtattact ccatctcggt atcaaaagaa tgaacaccga
     7441 caaggagtat gaaaagaaga ttgtcgaact cgccggaaag aggaaggatg gcaagcgtcc
     7501 tggtccggat gataccgtga gaaaacttag atagaaaatt aaggagcgag agaagaaggg
     7561 gaagtgattc ccaaccggcc cgggtaaatg atacccgggc cttttttaat attttttatc
     7621 aaggtcggac cttgataata tggcttaaaa tatatataaa aaccggcacc ctgccctgta
     7681 gtaagctttg ctttactaca gaggccccgc caaggcgggg gtaaacttga caaaaataat
     7741 gaaaggtgct atacttgtaa gagaagataa gttaataagt tctttgcatt caaataggcc
     7801 gcaaggcccc gattgaccag ggaggtcaaa atggacatcg tcgtcagcgt caaccaggtc
     7861 ctcaacacga tggacaaggt catcctcgcc aactgcgagg tgccgaagaa ggtcgcccgc
     7921 cgcaagaagg tgcacaagta cgagaaggcc agctactgtt ccgacgtcga gtccaagaag
     7981 cgccacatcg agaactccaa ccgcaaggcc gtcctcgacc agatctccga cgccctcgac
     8041 aaccgcacgc ccaacccgtt ctacaccatg agcgagcagg tcccgatcgt caccgtcacg
     8101 cccgcccaga tcgtcctcgc catcgagccc atcgattacc tcgggaacgt ggacggcgac
     8161 atcatcacgc acaacgggat gttcgtctcc gcctccttcg gggaggaggt ctcgcagagc
     8221 gagatcgtcg cgaacgcccc gatcgaggtc acgatcggcg aggacggcca gatcgccagc
     8281 gccagcttcg gcatcgagcc gaaggagacc ccggccaccc agcagatcgg ccaggtcctc
     8341 atcccggccg gcgacccgaa cgacttccgg cagaagcagc tccaggacat gaagccgctc
     8401 tactgctaga aggtctcgcg gtccctatag gacgtagcaa gcccctcgcg gctagctgcg
     8461 gtacggcaaa agggtttgcg ttacacagcg cgcccggccc catgcgaaag catggggttt
     8521 tctttaccct gttgagtaaa aatttctctc tgcaaagaga aattttaaat tttataaata
     8581 aaattttact taacagggtg aacttgacac ttttgtcttt atatgtataa ttgccaaaag
     8641 ctagttcatt tcacataaac ggt
//
LOCUS       DAOTEX010000035         8262 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3118
            NODE_6116_length_8262_cov_4.421104, whole genome shotgun sequence.
ACCESSION   DAOTEX010000035 DAOTEX010000000
VERSION     DAOTEX010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868142
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 8262)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8262)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 883
            CDSs (total)                      :: 833
            Genes (coding)                    :: 829
            CDSs (with protein)               :: 829
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8262
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6116_length_8262_cov_4.421104"
                     /isolate="AMR_MDS_3118"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            134..856
                     /locus_tag="P5056_02775"
     CDS             134..856
                     /locus_tag="P5056_02775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62667.1"
                     /translation="MFGIYSHLSDRLVIEIIGPTKNNHDLVRAILKHHRDLMTLNREV
                     GDYLDREEEICETDWFFADEEFLQRRQDKMNLEVGWPIVEEDVPKMNEKARKRGLGKK
                     GRLLAERRYRANGRKGDNSETDHFSHGRFLDKMARIVSTNDLDASFSEKKQEKQEALR
                     GTNPWRIEYVPPMAEESLVLDDSDKARFFLKNGLQFAEINSPDDDVDDHDEWRPQCED
                     DAVPFMSPGDYLDQNSKFAFEF"
     gene            complement(1013..2998)
                     /gene="rnr"
                     /locus_tag="P5056_02780"
     CDS             complement(1013..2998)
                     /gene="rnr"
                     /locus_tag="P5056_02780"
                     /inference="COORDINATES: protein motif:HMM:TIGR02063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HRY62668.1"
                     /translation="MMNKISMSKNRSKNNKKERKVGPRSELRSTTGEYLCTISLTKKG
                     EGFGMSPELKKDIFIASDKLLGAFHGDTARVRITNEKYFEGQVTKIETRAKEEFVGTI
                     HKEKGALWITPKDLKAPSGIMIVGGNTKDAREDDVALVRITDWGDKNTEPKGEIVRII
                     GRAGENDTEMESISEEHGFRTSHENVVTSEAKDLNESGIREKDLAGRRDFRDVVTFTI
                     DPEDAKDFDDALSIKHLGGDKFEIGIHIADVSHYVRPGTALDKEARKRATSVYLVDRV
                     IPMLPEILSNNLCSLLPNKDRLTFSAVFTMDNKGKIIDKWFGKTVIHSNKRFSYEDVQ
                     RVIEGEKNEYDKEIHTLQKIAKSLRAARFAKGSIYLDSAEIKFKLDEKGRPLSIYRKE
                     QKDAHKLVEEFMLLANKNVAEEIFNKGDKGGKIFIYRNHDLPDSDKIEDLKEMLVNFG
                     YKPRGKGTGLTGQEINAVIDALEGKSERSLVMWIILRSMAKAVYSTENIGHFGLAFDS
                     YTHFTSPIRRYPDTMVHRLLDAHLKNEKIRDDIKEYEKICNHCSEMEKKAMNAERESI
                     KFKQVEYVMDKIGQTFTGIITSVTDWGMYVEAEEILCEGMVALRDIKEDYFTLDKKGI
                     MLVGEKTHKRYKIGDRVKIELIAANLRRRTIDFRLIP"
     gene            3197..3673
                     /locus_tag="P5056_02785"
     CDS             3197..3673
                     /locus_tag="P5056_02785"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006691710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRY62669.1"
                     /translation="MYEATMKTNMGDIVLELYKDKAPVTVENFVKLSNSGFYNGTKFH
                     RVIKDFMIQGGDPNSKLADWSTHGMGGPGYAFEDEINDIKLVAGVLAMANAGPNTNGS
                     QFFIVTAESTPWLDGKHTAFGKVIGGMDIVKKIEDVATGQNDHPLEDVVVESISIK"
     gene            3877..5037
                     /locus_tag="P5056_02790"
     CDS             3877..5037
                     /locus_tag="P5056_02790"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRY62670.1"
                     /translation="MKFLKNLALVSILLVVIFPLTSSAETEVRITGSGPILAIDEPNV
                     RVNIDLGLFVEVAGIALEKHIDRDKFNSQKLGIGVARGFLNSIKIYKLTLNGIAENAR
                     KPRTAERSLRITSGGRSIDLDLGILIEEIRLSAESGKLGRNPDKNAIVVGMIKGFLGS
                     LDRYSYFATNKEEVEHRKMLDGKPEEKGGPEPQTVACGIMNISGHKVGIIKIVIFDKV
                     TQNQYERDMKELLALNPEYLIYDLRDNPGGYLQTVGEMLAMTVGKGKVIFSTEEEPGK
                     LIHVRGTNESGEGLVPSAKTRIKKIVCLINRGTASSGEIMTAGLKDVLGARLVGERTY
                     GKGTALDPCKLKSGRGVCYIVAARWYTPSGKCVDGTGIIPDVMVRFVPQGTF"
     gene            5199..6362
                     /locus_tag="P5056_02795"
     CDS             5199..6362
                     /locus_tag="P5056_02795"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRY62671.1"
                     /translation="MQFFYGTVIALLTVAFIVLFSLTGWCGGDIPVLPVSSILKITEN
                     GNSVKVDLGILMEAMQIASSYHVDRKSLDKDKMVGGMIRGFLMYINDAQRKSLLVAAK
                     NCPYKPTDCKDQLIFRVVADDGFVLRVDAGYFLWTLKLAVREGLLKKKRNGPGLAECL
                     AMGFVRSLDRHSDFLDPEEAKDYIKSFELGNTVSVRSLGDKSNKVYIVYIVEFARNTA
                     REFKKLTKELSREKNARVIFDLRDNPGGLDIPTEEILGYLVGANKVLLSEKDYSGVEV
                     FKRTPIDSGAGIANKWKIVCLVDNGTASNAEVFSACLRDNLGAKLIGTTTYGKGVGGT
                     LYDLRSGRGSCRITYTRWYTPCGKCIQDVGLLPDIEVGDELGQMNRAIQELLK"
     gene            6471..6809
                     /locus_tag="P5056_02800"
     CDS             6471..6809
                     /locus_tag="P5056_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62672.1"
                     /translation="MKRALTILGFGICPVAFLVILELLLNLKNPSFEFVVPWMTIISW
                     VVLFCISFDIARSTSVNSLDMAIPPAVVGMIFGVFYWGIPCLETLPFLFGWFLGIVIG
                     MPMRKKEPSQ"
     gene            complement(6841..>7579)
                     /locus_tag="P5056_02805"
     CDS             complement(6841..>7579)
                     /locus_tag="P5056_02805"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF012760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HRY62673.1"
                     /translation="LTPPPAKVWAEKRGIPVLQPKSLKDFEISGDYDLFVLAAYGKII
                     PKRILELPKGGILNVHPSLLPLLRGPSPIQSAILSGAKETGVSIMLLDEEMDHGPVLD
                     SEKLSIEDKPSYSELEKKLGKIGGKKLCEIIPKWVRGEIKAEAQDHSSATYCKKIEKQ
                     DGYIKPGIITGIETDREKHAEAERKVRALNPDPSTYTILNIRDKEMRIKITEAKTEDG
                     KFVPVKVIPEGKKEMKWEDFLKGNLVK"
     assembly_gap    7580..7589
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(7729..>8262)
                     /gene="def"
                     /locus_tag="P5056_02810"
     CDS             complement(7729..>8262)
                     /gene="def"
                     /locus_tag="P5056_02810"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HRY62674.1"
                     /translation="IVQMEDPVLRQKAKEVAPEEIGTPKLNKIIEDMKKAVDTCDDGV
                     AIAAPQIGVSLRIFVVSPKAFIAPDEEITDEKLKKFKHLVFINPKIKKLSSKTTQLDE
                     GCLSVRGVFGKIRRSEKASIEAIDENGKKVSRGAGGLLAEVFQHETDHLDGVLFVDKA
                     TDLYQLTENKDQKKKNG"
BASE COUNT         1962 a         1844 c         1989 g         2457 t
ORIGIN      
        1 cataaggaat cgcccgccaa aactttttaa caaaaagatt tagtggacac aaaggtcaat
       61 ctgggtgttt aaaagactcc cagccgttac ctcttgaaca gtgaaaagtt taggagggca
      121 aggaggttgc tgtatgttcg gtatctacag tcatctgagt gatcgtttgg tcatcgagat
      181 catcggcccc accaagaaca accacgatct cgtccgggcc attctcaagc accatcgcga
      241 tctcatgacg ctcaaccgag aggtcggcga ctatctggat cgcgaggaag agatctgcga
      301 gaccgattgg tttttcgcgg acgaggaatt tctccagagg agacaggaca agatgaacct
      361 cgaggtcggc tggccgatcg tcgaggagga cgtccccaag atgaacgaga aggcgcgtaa
      421 gcgcggtctc ggcaagaagg gccgtcttct cgcggaacgt cgctatcgcg cgaatggccg
      481 gaagggagac aattccgaaa ctgaccattt ttcccacggt cgttttctcg ataaaatggc
      541 acgcatcgtg tcgacgaacg acctcgacgc ctcgttctcc gagaagaagc aggaaaagca
      601 ggaggctctt cgcggcacga atccctggcg gatcgaatat gtcccgccga tggccgagga
      661 gagccttgtg ctcgacgatt cggataaggc gcggttcttt ttgaaaaacg gtcttcagtt
      721 cgctgagatc aattcccccg atgacgacgt ggacgaccac gatgaatggc gtccgcagtg
      781 cgaagatgac gctgtcccgt tcatgtcacc cggggattat ctggaccaga acagcaagtt
      841 tgcgtttgag ttctaaaaac acgccgcctt acacgaaagt gttaaggcgg cttttctttt
      901 tttagtggtt ctggattccc gccttcgcgg gaatgacccc ggtagcagag cgaagctcgc
      961 tacggggcag gcagaacgga catgggttgc ttcgctttgc tcgcaatgac ggctaaggta
     1021 tcagccggaa atctatcgtt cttcggcgga gatttgccgc gatgagctct atttttactc
     1081 tgtcgccaat cttgtatctc ttgtgagttt tctctccgac gagcattatt ccttttttat
     1141 ccaaggtgaa ataatcttct ttgatatctc ggagagccac cattccttca cagagtattt
     1201 cctcggcttc tacatacatt ccccagtccg tgacgcttgt gatgatgccg gtaaaagttt
     1261 ggccaatttt gtccatcaca tattccacct gtttgaattt gattgactct ctttcggcgt
     1321 tcatcgcctt tttctccatt tcagaacaat ggttgcagat cttttcgtat tcttttatat
     1381 cgtctcttat tttttcattc ttcaaatgag catcgagcag tcggtgcacc atagtgtcgg
     1441 ggtagcgtct tatcggcgac gtgaagtggg tatatgaatc aaaggcaagt ccaaagtggc
     1501 cgatattttc tgtagagtaa acggccttgg ccatagacct gaggataatc cacatcacga
     1561 gagacctctc gctctttccc tcaagggcat cgataaccgc atttatctct tgacctgtta
     1621 agcctgttcc ttttccgcga ggcttatatc cgaagttcac aagcatttct ttgaggtctt
     1681 caattttgtc gctatcaggg aggtcgtggt ttctataaat aaatattttg ccgcctttat
     1741 ctcctttgtt gaatatctcc tcggctacat ttttgttcgc gagcaacata aactcttcaa
     1801 caagcttgtg ggcgtctttt tgctcttttc tgtatatcga gagaggcctt cctttctcat
     1861 ccaacttaaa ttttatttcc gcagaatcaa gatagataga gccttttgca aaccttgccg
     1921 ctcttaaact ttttgcgatc ttttggagag tgtgtatttc tttgtcatat tcattttttt
     1981 ctccctctat cactctttgg acatcttcgt atgaaaatcg tttatttgaa tgaatgacgg
     2041 ttttgccgaa ccacttgtct attatttttc ctttgttgtc catcgtgaat acggccgaga
     2101 aggtgagtcg gtctttgttc gggagcagag aacagaggtt gtttgaaagt atttcgggga
     2161 gcatcggtat aaccctgtct acaagataga ccgacgtagc tcttttgcgt gcctctttgt
     2221 cgagggctgt gcccggtctt acataatgag acacatcggc aatatggatg ccaatctcga
     2281 atttgtctcc gcccaggtgc tttatagaga gcgcgtcgtc aaagtctttt gcgtcctcgg
     2341 ggtctatggt aaaagtgacg acatctctga agtcgcgtct gccggcgaga tccttttctc
     2401 ttatgccgct ttcattcaga tcttttgctt cgctcgtcac gacattttca tgagaagttc
     2461 tgaagccatg ctcttcggag atagattcca tctcggtgtc attctctccg gcacgcccta
     2521 taatacgaac aatttctcct ttaggctcgg tatttttgtc tccccagtct gtgatcctga
     2581 caagcgcgac atcatcttct ctggcgtctt tagtgtttcc cccgactatc attatcccgg
     2641 aaggtgcctt caggtctttt ggtgttatcc aaagagcgcc tttttcttta tgtatagtgc
     2701 cgacgaactc ttctttggcg cgagtttcta tttttgttac ctggccttca aaatactttt
     2761 cgttagtgat ccttactctt gcggtatcgc catgaaatgc gccaaggagt ttgtcagatg
     2821 cgataaaaat atctttttta agctcagggg acattccgaa tccctctccc ttttttgtaa
     2881 gggaaatagt gcacaaatac tccccagtag tagagcggag ctcgctacgg ggcccgactt
     2941 ttctttcttt tttattgttt tttgatctat tcttgctcat tgaaatttta ttcatcatat
     3001 cctatcatat ttagatatat tagcattaaa gtgataaaac atggagaata aaaatactta
     3061 tattgcagtg gcggtcattc ttgtggcgct atcgtatctg gtattttggg gaggttcggc
     3121 ggggaccacg cctacgcccg cagaagagcc gaaaatagag attaacacta atgaggtaaa
     3181 aatagaagac aaaaatatgt acgaagcaac aatgaagaca aatatggggg atatcgttct
     3241 tgagctttac aaggataaag cccctgttac cgtagagaat tttgttaaac tttctaactc
     3301 aggattttat aacggcacaa agttccacag agttatcaaa gattttatga tccagggagg
     3361 cgacccgaac agcaagctgg ccgactggtc cacacatgga atgggaggac cggggtatgc
     3421 ttttgaggac gagataaatg acataaagct tgtagccggg gtgcttgcaa tggccaatgc
     3481 cggaccgaat acgaacggaa gtcagttctt cattgtaact gctgaatcta cgccatggct
     3541 tgacggcaaa catacggctt ttggtaaggt catcggagga atggatattg taaaaaagat
     3601 tgaggatgtc gctaccggcc agaatgatca tcctttagag gatgtggtcg tagagagcat
     3661 ttctataaaa taatcagtct aagtggacaa aaatagagcc ccgtgtttat ttgcacgtgg
     3721 ctctttttct taattctatc gtgatgtagc atttatatag ataagtgtcc tttgaaaaaa
     3781 gaacgcagta taaccaaaat aaagaaagga gttgtttggt tcggggtctc gcaatttatt
     3841 tgagaataat caacaagaaa aaggaggcag agagatatga aatttttgaa gaacttggct
     3901 cttgtttcta tccttctcgt tgtcattttt ccgctcactt cttcggccga aaccgaagtt
     3961 cgtattaccg ggtcgggtcc cattctcgca attgatgagc ctaacgtcag ggttaatatt
     4021 gacctcggcc tttttgtcga ggtcgcggga attgctctcg aaaaacacat tgatcgcgat
     4081 aagtttaatt cacaaaagct cggaatcggc gtcgcccgtg gtttcctgaa cagcatcaag
     4141 atctacaaac tcaccctgaa cggtatcgcc gaaaacgcgc gcaagccgag gactgccgaa
     4201 aggagtcttc gtattacgag tggtggccgc tcgatcgacc ttgatctcgg tattcttatc
     4261 gaagagatcc gactctcggc tgaatcgggc aaactcggta ggaatccgga caagaacgct
     4321 atcgtggtcg gaatgattaa gggcttttta ggaagcctcg atagatattc ctatttcgcc
     4381 acgaacaaag aagaggtgga gcaccgaaag atgcttgacg gtaagcccga ggaaaagggc
     4441 ggaccggaac cccagactgt ggcttgcgga attatgaata tttccggcca caaggtcggc
     4501 atcatcaaga tcgttatttt tgataaggtt actcagaatc agtatgaacg cgacatgaag
     4561 gaactcctcg ctctgaaccc tgaatatctt atttatgatc ttcgcgataa ccccggcggc
     4621 tatctgcaga ctgtcggaga gatgctcgcg atgaccgtcg gtaaagggaa ggttattttt
     4681 tccaccgaag aagaaccggg caagctgatc catgtcagag gaaccaatga gtccggtgaa
     4741 ggactcgtcc ctagcgccaa gacgcggatc aaaaagatcg tttgtctcat caaccgcgga
     4801 accgcctctt cgggcgagat catgaccgcc ggcctcaagg atgttctcgg tgcgaggctc
     4861 gtcggcgagc gcacttacgg caaaggaacg gctctggacc cctgcaagct caaatccggt
     4921 cgcggtgtct gctatatcgt ggccgcgcgc tggtacacgc cgagtggcaa gtgcgttgac
     4981 ggcacgggga ttatccccga tgtcatggtg cgttttgtcc ctcaaggaac tttttgaagg
     5041 gaaagatccc caaaaggacg ctgcgtttaa agaattgttc aagtagcatt ttacccccca
     5101 ccacttttgg ttggggggat tttcttttag accatttatg tgatagcgtt ttatataggg
     5161 agagtaactt gaaaatttaa ttttaaggag ggattgttgt gcagtttttc tatggtacgg
     5221 ttatcgctct tttgactgtc gcttttatcg tattatttag ccttacaggc tggtgcggag
     5281 gtgatatccc tgtgttgccc gttagctcga ttctcaagat tactgaaaat ggtaatagtg
     5341 ttaaggttga tctcggaatt ttgatggagg caatgcagat cgcctctagt tatcacgtgg
     5401 atcggaaaag tcttgataag gataagatgg tcggcggaat gattcgcgga tttctaatgt
     5461 atattaacga tgctcagagg aaatctctac tcgttgccgc aaagaattgc ccgtataaac
     5521 cgacagactg caaggatcaa ctcatatttc gagtggtcgc ggatgacgga tttgtccttc
     5581 gcgttgatgc cggatatttc ttgtggacgc ttaaacttgc tgtccgcgag ggcctcttaa
     5641 aaaagaaaag gaacggcccg ggtctggccg agtgcctcgc tatgggcttt gtgagaagcc
     5701 ttgatcgcca ttctgacttt cttgatcccg aagaggccaa agactatatc aagagttttg
     5761 agctgggaaa taccgtttct gtccggtctc tcggcgacaa gagcaacaag gtatatatcg
     5821 tttatatcgt tgagttcgcg aggaataccg caagggaatt taagaaactc actaaggagc
     5881 tttctcgaga gaagaacgcg cgcgtgatat tcgatctgcg cgacaatccg ggagggctgg
     5941 atatccccac agaagagatt ctcgggtatc ttgtcggcgc caacaaagtg cttctttcgg
     6001 agaaggatta ttccggtgtt gaagtcttta agcggacgcc tatcgatagc ggggcgggga
     6061 ttgccaataa gtggaaaatc gtctgtcttg tggacaacgg gacggcttca aacgctgaag
     6121 ttttctccgc gtgccttcgc gacaatttgg gcgccaaact tatcggaact accacttacg
     6181 gtaagggtgt cggcgggaca ctctacgacc tcaggtccgg taggggttct tgcaggatta
     6241 cttataccag atggtatacg ccttgcggta agtgcatcca ggacgtcggt cttttgccgg
     6301 atatcgaagt tggagatgaa ttgggccaaa tgaatcgtgc gattcaggag ctccttaaat
     6361 agaagaatga agttcgccgt cctctcgtag ggcggctttt ttattgctga cagaagacgt
     6421 ctgtgatagt gttaaaccaa aataaagttc cttgaaaggg gacggttctg atgaagagag
     6481 cgttaacgat tttgggtttt ggcatctgtc cggtcgcttt cttggtcatt ttggaattac
     6541 ttctcaattt aaaaaatcct tctttcgaat ttgtcgttcc gtggatgacg attataagct
     6601 gggtcgtgct tttctgtatc tcttttgata ttgctcgctc aacgagtgtt aattctcttg
     6661 atatggcaat ccctcccgct gttgtcggaa tgattttcgg agtattttat tgggggattc
     6721 catgtttgga gacactcccg tttctgttcg gatggttcct cgggattgtc ataggtatgc
     6781 caatgagaaa gaaagaaccc tcacagtagc aatagttcgc cgccctctcg cagggcggtt
     6841 tcattttacc aaattgcctt ttaagaagtc ttcccatttc atttcttttt ttccttcagg
     6901 gatgactttt acggggacga attttccatc ttccgttttt gcttcagtaa tttttattcg
     6961 catttctttg tcgcgaatat ttaatatcgt gtatgtgctt gggtcaggat ttagggcacg
     7021 cacttttctt tctgcttcgg cgtgtttttc cctatctgtc tctatgcctg taattattcc
     7081 cggcttgata tatccgtctt gcttttcaat ttttttacaa tatgtcgcgc tagagtggtc
     7141 ttgggcttcg gcttttatct cgccccgcac ccactttgga attatttcgc aaagtttctt
     7201 gccacctatc tttccgagtt ttttctcaag ctccgaatat gacggcttat cttctatact
     7261 caatttttcc gaatcgagca caggcccgtg atccatctct tcgtccagga gcattattga
     7321 aacgccagtt tcttttgctc cgcttaaaat tgccgattgt atagggcttg ggccgcgaag
     7381 taaggggagg agagacggat gcacgtttag tattccgccc tttggcaatt ccaagattct
     7441 tttcggaata atttttccgt atgcggccag gacaaaaagg tcgtagtcgc cggagatttc
     7501 aaaatctttt aagctttttg gctgaagaac agggatgcct cttttttctg cccaaacttt
     7561 cgcgggcgga ggagtgaggn nnnnnnnnng acgaggtgtc tgccttttgg cttgtccgga
     7621 gaagtgatta ctttatccgg caaaagcccg caggcctcaa gctcgtccaa aatgaggacc
     7681 gcaaaattag gcgttccgaa gaataaaaat ttcggtttat ttgagtcgct agccattctt
     7741 tttcttttgg tccttgtttt ccgttagctg atacagatcc gtggctttat ctacaaaaag
     7801 cactccgtct aggtggtctg tttcgtgttg gaaaacttcg gcaagaaggc cgcctgcgcc
     7861 gcgagatact tttttaccat tttcgtctat cgcttctatc gatgcttttt cagaccttct
     7921 tatctttccg aatactccgc gcacgctgag acacccttcg tctagctggg tcgtttttga
     7981 agaaagtttt ttgatctttg gatttataaa aacgagatgc ttaaatttct ttaatttttc
     8041 gtcggttatt tcctcatcgg gtgcgatgaa cgcttttgga gaaaccacga atatcctgag
     8101 agacactcca atttgagggg cggcaatagc gacaccatca tcgcaggtgt ccacagcctt
     8161 tttcatatct tctattattt tattcagttt tggagttcca atttcctccg gggccacttc
     8221 tttggccttc tgtctcaata ccggatcctc catttggacg at
//
LOCUS       DAOTEX010000036         8133 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3118
            NODE_6237_length_8133_cov_4.195345, whole genome shotgun sequence.
ACCESSION   DAOTEX010000036 DAOTEX010000000
VERSION     DAOTEX010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868142
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 8133)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8133)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 883
            CDSs (total)                      :: 833
            Genes (coding)                    :: 829
            CDSs (with protein)               :: 829
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8133
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6237_length_8133_cov_4.195345"
                     /isolate="AMR_MDS_3118"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..435)
                     /locus_tag="P5056_02815"
     CDS             complement(<1..435)
                     /locus_tag="P5056_02815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62675.1"
                     /translation="MKFLNVPTRNYILGAVLTILLFMSLAGAKVSIFLYIFAAAGSVP
                     VFVKAFKDALSFKISIEVFNAFVLSVAFYLAEPDSAAFITLMLTFADYLDFITETRAH
                     HAIEELLKLNPITATIEKGEALVETAIDKIVEGDIVLIKSGDR"
     gene            493..813
                     /gene="raiA"
                     /locus_tag="P5056_02820"
     CDS             493..813
                     /gene="raiA"
                     /locus_tag="P5056_02820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HRY62676.1"
                     /translation="MNINIKGTGIELTPEIRSYLDKKIASLEKFVTEEDTGATANIEI
                     GKTTNKHKSGIIFFTEINFTIRKADLRVKAFGDSLMASMDKAKDMALERLRTEKDRTA
                     SHQL"
     gene            complement(855..1391)
                     /locus_tag="P5056_02825"
     CDS             complement(855..1391)
                     /locus_tag="P5056_02825"
                     /inference="COORDINATES: protein motif:HMM:NF014849.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HRY62677.1"
                     /translation="MNEEKRKVFLALRVPDEIKYNVTEVKNKYHNFPVRWNSDKDLHI
                     TVIAPWLEGDIPALLEKLKGIEGKVGRINILFDTVAYGPNNFSPRLIWTTGDVPQKIL
                     DLRVMLKDALAITNFRNDFFTHLTLARFEPADIERMPMRRIREEVRWRGVADTLLLIE
                     SKPGTGGPDYEVLAEIKL"
     gene            1479..2177
                     /locus_tag="P5056_02830"
     CDS             1479..2177
                     /locus_tag="P5056_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012979455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HRY62678.1"
                     /translation="MNDKILETKDLSVTLEGRQVIQDISLSIERNTILAIIGPNGAGK
                     SVLLRTLLGLVPHSGEVKWQKGVKIGYVPQKVSIEKDFPLSVIEFLKLKKNADGVEDA
                     LQNTGLKGHQILHRQISDLSGGELQRVLIAWAIIGNPDILLFDEPLTGIDIGGEETIY
                     HLLGKLKKERGTTIILVSHDISVVYKYADTVLCLNKKQICLGKPNEVINKDTEKKLYS
                     ELEGFYKHDHEHHK"
     gene            2179..2949
                     /locus_tag="P5056_02835"
     CDS             2179..2949
                     /locus_tag="P5056_02835"
                     /inference="COORDINATES: protein motif:HMM:NF013143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HRY62679.1"
                     /translation="MTNELNLLSLATGIFVGGAAGYIGSFMVLRRMSLVGDALSHVAL
                     PGLAIALLFNINPFIGAFSFLFVATLSIWVMEQKTTLPIDTIFGVFFTASLAIGILIT
                     PEAELLEALFGDITKITPIEAAVAIILSIVIIAATRAISKNELLGIISNDLAISEGVN
                     ISKTNLAYLMLVALIVALGIKAVGTILTGALVIIPAASAKNIASNFRDYSFYSLLFGS
                     LSAVIGIIVVQQTGLSSGPVVILSSVAIFILSLIFKKE"
     gene            complement(2951..3130)
                     /locus_tag="P5056_02840"
     CDS             complement(2951..3130)
                     /locus_tag="P5056_02840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62680.1"
                     /translation="MFGISGWAEKPFRPAPIKEGGEIDPVAPIDKNREEGERSDGERR
                     ENLEPHKGQLVDIEA"
     gene            complement(3130..3339)
                     /locus_tag="P5056_02845"
     CDS             complement(3130..3339)
                     /locus_tag="P5056_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62681.1"
                     /translation="MTLQTAVYLLLAILVGGTLFAWYNVYLEYFAMCKTCSVPGTSLL
                     WSKCFWGAFFFTAALLTAYYIKTLI"
     gene            3449..3520
                     /locus_tag="P5056_02850"
     tRNA            3449..3520
                     /locus_tag="P5056_02850"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3481..3483,aa:Asn,seq:gtt)
     gene            3953..4549
                     /locus_tag="P5056_02855"
     CDS             3953..4549
                     /locus_tag="P5056_02855"
                     /inference="COORDINATES: protein motif:HMM:NF023806.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TasA family protein"
                     /protein_id="HRY62682.1"
                     /translation="MKKIVLSVAIIALSTTVIVGATRAYFSDTETSTGNTFAAGTLDL
                     NLDGADTNSVKFTVTNTAPGASGTSYWTVKNVGSLAGYVDLASIAKTNDDNLCTEPES
                     TDGDTTCGAGQGELGANMNITIFVDADKDGVIDGGETTLYTGTLDGLATSYDANLALA
                     AGAEQYVSLNWSIPTTVGNVIQSDSVQLDMSFQLGQTI"
     gene            4615..5148
                     /locus_tag="P5056_02860"
     CDS             4615..5148
                     /locus_tag="P5056_02860"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HRY62683.1"
                     /translation="MNKTYGKFEKFAYVVIFIITAVLLWLAMPFEKSPKLFIVLSGSM
                     EPEIHTGSVVVTIPQNKYRVDEIVTFKTGKNDKITTTHRIISEKTIDQGVYFTTKGDA
                     NNTPDMEEIPSENILGRVVFSISKLGYILDFIRRPISILIIVIIPAIFIIFDETKKII
                     EEVGKMKDGKNKIIKKK"
     gene            5117..7306
                     /locus_tag="P5056_02865"
     CDS             5117..7306
                     /locus_tag="P5056_02865"
                     /inference="COORDINATES: protein motif:HMM:NF033679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HRY62684.1"
                     /translation="MAKIKLSRKNKKKEEETFWADFLLGFVPVFILYVMIAGSVIVSK
                     TRAILQDNQSSIGSIIQAGILDFSLNQPSSFSSAPLQKGESVGVDVSLLNDGNLNFQY
                     IASTTIMGGDQDLCSALTLEASLNGATIYDNSVSEFASPLQNYVNPSDWGLSVTLPDT
                     ASDSLQNKSCNLRFVFSGWQNGLSSNQGFNNLEFIDVTINSGEWIIPEPGDLNMSPIS
                     DSYIRQDRATTNYGGGTTMLVQSRSGSRNYRAVIRFNFKLPSGVIIDSSFLKLYMTSA
                     PSASRNYEARSLLGSWTETGVNWSNQPASSASPSDTITTGTTRNLWMSWNVKNDVQSI
                     VNGAANYGWLIKDVTENSSMTRGATFASRENSTVGVRPVLEVAFSAPPASTDHLVVNE
                     VYYQVAVGKGVETTDEWVELYNPTTSDIDLLGWKICDNGGCDTLGSYTLPSRSFAVVT
                     PNTSNFDTYWTLAPGAIKITLPNTNIGGNGLGNTGDRIYIQNASSTAVDGISYGTDVS
                     QLNPPVSTGGLGSSLARIIKGYDFNVADDWITNSTPNPGTNPGSDSKEIMRFTDYGTE
                     VAASSVGLSPLITNYIPADTDMSGEEETPTEIFKQCSAGSMPVAKEEATFTEKTGGTI
                     EPVAASSTTTDPIIEPDISTTTPEIITDILPAEATSTDQTVDTNTPSEPETTEALPGT
                     SPADTNVIVELPTAETETTQDVLIAEEQAPIAEPAQEIDPVETPTEQ"
     gene            7308..>8133
                     /locus_tag="P5056_02870"
     CDS             7308..>8133
                     /locus_tag="P5056_02870"
                     /inference="COORDINATES: protein motif:HMM:NF027967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003993 - acid phosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HRY62685.1"
                     /translation="MKINIQTNIALRVILAVFMLFAVHAKAADPTVLFEQTPLFSGTN
                     FAPGETVTRWIKITNNTDEAHMSVLKAVNLASVKDLGSQIKIEINEGGSTIYSGSFSD
                     LFNLSELKLKEIGPGTQKKFNLTATFLPDSKDSYQGGSLSFNIKTGLEDVIIVSDAPP
                     VGGGGPMAPVTEKITFSEIFLEQNNGDDSLNVKWNTNLPATSRVVYGPVSMGPYSANL
                     NSQNIGYLLSTKENPLPQNTHSVELTGLIPGEEYYFRIISEAGMFNASQEYVLKLDK"
BASE COUNT         2500 a         1824 c         1676 g         2133 t
ORIGIN      
        1 cctgtcgccg ctttttataa gcacgatatc tccctcgaca atcttatcaa tcgccgtctc
       61 caccagagcc tcccctttct caatagtcgc cgtaatcgga tttaatttca aaagctcctc
      121 tatcgcatga tgagctcgag tctcggtaat aaaatcaaga taatctgcga aggtaagcat
      181 cagcgtaata aaagccgcgg agtccggctc cgccaaataa aaagcgacgg acagaacaaa
      241 agcattaaac acttctatgc ttattttgaa cgacagcgca tccttaaaag ccttcacaaa
      301 cacaggcaca gaaccggcag cggcaaatat atataaaaag attgatactt ttgcgcccgc
      361 aagcgacata aaaagcaata tagtaagaac ggcacccaaa atataattcc ttgtcggaac
      421 atttaaaaat ttcattgtta aattataaca aagtttgcta aaatttgtat gttactcgtt
      481 aattcttttt ttatgaacat aaatattaaa ggaacaggta tagaactcac tccggagata
      541 cgtagctatc tcgacaaaaa aatagcttcc ctggaaaaat tcgtcacgga agaagacacc
      601 ggcgccacag ccaacataga aatcggcaaa actaccaata agcacaaaag cgggataata
      661 ttctttactg aaataaattt tacaattaga aaagcggatc ttcgggttaa ggcttttggc
      721 gatagcctaa tggcctcaat ggataaggcc aaagacatgg cgctagaaag actgcgaaca
      781 gaaaaagaca gaacagccag ccatcaacta taacacaaaa gcccctttcg gggctttttt
      841 atttgtcgat tatattaaag cttgatctcc gccaatacct cataatccgg cccgccggtt
      901 cccggctttg actctattaa aagcaaagtg tcggcaaccc ccctccatct cacctcttct
      961 cttattcttc tcatcggcat tctctctata tccgcaggct caaacctggc cagtgtaaga
     1021 tgtgtaaaaa aatcgtttct gaaattagta atagcgagag catccttaag cattaccctc
     1081 agatccaaaa ttttttgagg aacatctccc gttgtccaga taagtcttgg ggagaaatta
     1141 ttcggcccat aagctacggt atcaaaaaga atattgattc taccaacctt gccctctata
     1201 cccttaagtt tttccaaaag cgcggggatg tctccctcaa gccatggcgc gataactgta
     1261 atatgcagat ccttatcgga gttccatctt acagggaaat tgtgatactt attttttacc
     1321 tcagtaacat tgtattttat ttcatcagga accctgagtg ccaaaaatac cttccttttt
     1381 tcttcattca tataatagat aattacaagc tgatattact ttattaccct ttataacata
     1441 ttcataaacg attgagaata tgttacgata taaaaacaat gaatgacaaa atattagaaa
     1501 caaaagactt gtcggtcacc ctagaaggaa gacaggttat acaagacatt agcctctcca
     1561 tagaacgaaa taccatatta gccatcatcg gccctaacgg cgccgggaaa agcgttttat
     1621 taagaacgct cctcggtctt gtgccgcatt cgggtgaagt taagtggcaa aaaggtgtaa
     1681 agatcggtta tgtcccccaa aaagtgtcta ttgagaaaga ttttcctctt tctgttatag
     1741 aatttcttaa gctcaaaaaa aatgccgatg gcgtcgaaga tgcccttcaa aacacagggc
     1801 taaagggaca ccagatactc catagacaaa taagcgacct gtccggagga gaactacaaa
     1861 gggtcctcat cgcatgggcc attataggga acccggacat ccttcttttt gacgagcccc
     1921 taaccggtat agatatagga ggagaagaaa ctatctatca tcttttggga aagttaaaaa
     1981 aggagcgagg cacgacgata atactcgtat cccacgatat aagcgttgta tacaaatatg
     2041 ccgatacggt gctgtgcctt aataaaaaac aaatatgttt aggcaaaccg aacgaagtaa
     2101 taaacaaaga cacggagaaa aaactttata gtgaattaga aggtttttat aagcacgacc
     2161 acgaacacca taaataaaat gacgaacgaa ctaaacttat taagtcttgc tacgggaatt
     2221 tttgtcggcg gagcggccgg ttatatcggt agttttatgg tccttagaag aatgtctctc
     2281 gtcggggatg ccttatctca cgtagcgctt cccggccttg ccatagccct tctttttaat
     2341 ataaatccat ttataggagc gttctcgttc ttatttgtcg ccactctaag catttgggtg
     2401 atggaacaaa aaacaactct tccgatagat acgatttttg gagtattttt cacggcgagc
     2461 ttggccatag gtatcttgat aacgcctgaa gccgaattac ttgaggctct gttcggcgac
     2521 ataacaaaaa ttacacctat cgaagccgct gtcgccataa ttctctcaat cgtaataatt
     2581 gccgcaactc gggcaatatc aaagaacgaa ctgctgggga taatttcaaa cgatctcgcc
     2641 atatctgagg gggtaaatat ctcaaagaca aaccttgcct accttatgct tgtcgcactc
     2701 attgtggccc tgggcattaa ggccgtcggg accatcctca cgggagcgct ggtcataatc
     2761 cccgcggcat cagcaaaaaa catcgcttca aattttagag attattcttt ttacagccta
     2821 ctattcggct cactaagcgc cgttatcggg attatcgtag tgcaacaaac cggactctct
     2881 tccggtccgg ttgttatact ttctagcgtc gctattttta ttctttctct tatatttaag
     2941 aaggagtagg ctaggcttct atatctacta gttggccctt gtggggttct agattttcgc
     3001 gcctttcacc gtcgcttctt tccccctcct ctctattttt gtctatcggc gcaaccgggt
     3061 ctatctcacc gccttctttt attggagcgg gtcgaaacgg cttctccgcc cacccggaaa
     3121 taccaaacat tagataagcg ttttgatata atatgcggtc aaaagagcgg cggtgaaaaa
     3181 gaaggctccc cagaaacact ttgaccacaa aaggcttgtg ccgggcacag agcaggtctt
     3241 gcacatagca aaatattcta aatatacatt ataccaagca aaaagcgttc ctccgacgag
     3301 gatagctaaa agcagataaa cggctgtttg tagagtcatg ttaataaaat taaacttata
     3361 attattaatt atacatctcg cctaaatatt ttgcattttc tctaggattt gataagataa
     3421 ttctcagttc ttttatagag caaaacattc cgcggtagct cagtggtaga gcgaccgcct
     3481 gttaagcggt tggtcgcagg ttcgaatcct gcccgcggag taaaacgaaa aacccccttt
     3541 tgggggcttt ttcgttttac gaagtggagc aaacaaactg ctttgtttgc gggcaggatt
     3601 cgaaaaggtt ctccgttata ttttagaaat ttttatttct aaaatatcgg aaacctatac
     3661 cgtcctcgta gaggaagaat cctaacagat aaatggcttt cgtcaggatt cgaagctgtt
     3721 ctcccttatt tcttaggatt ctttaatccg aagaaatagg aaacgggtat tggccctgta
     3781 gggaccgaat cctacctccc aataacctag acttattttt aatttttaga tatctttgta
     3841 tttagtagta agagggtggt tatttagcat taactgattt gataggtcgc aaaatcagta
     3901 ttttattaaa aattatatta taagaagttt ttatttatta aataaacaaa ccatgaaaaa
     3961 aattgttttg agcgtcgcca ttatcgcact gtcaacgaca gtaatagtgg gcgccacaag
     4021 agcatacttt agcgatacgg agacctccac aggtaacacg ttcgcggcag gaactttgga
     4081 tttgaacctc gatggtgcag atacaaattc tgttaagttc acagttacaa atacagctcc
     4141 cggagcttcg ggaacaagtt actggacagt taagaacgtc ggaagtctcg ccggatacgt
     4201 tgatctcgca agtatcgcaa aaacaaatga tgataatttg tgcacagaac ctgagtcaac
     4261 agacggagac acaacttgcg gagccggaca gggagaactg ggtgcaaata tgaatatcac
     4321 aatctttgtt gatgccgaca aggatggcgt tatcgacggt ggagaaacca ctctttatac
     4381 cggcactctt gacggtttag ctactagcta cgacgccaac ctcgctctcg cagcaggcgc
     4441 cgaacaatat gtctctctca actggagcat acctacaacg gttggtaatg ttatccagag
     4501 cgacagcgtt cagctcgaca tgtcattcca gctcggacaa acaatatagt tctgtttcag
     4561 ctttacctct ccccgcttat ttaataaacg gggagtctga agagctgaaa ttgcatgaat
     4621 aaaacttacg gtaaatttga gaaattcgct tatgtcgtaa tttttataat tacggctgtt
     4681 cttctctggc tggcaatgcc gtttgagaaa tctccaaaac tatttattgt gctttccggg
     4741 tcaatggagc cggagataca tacgggcagt gttgtcgtca cgatacctca aaacaaatac
     4801 cgagtagacg agattgttac ttttaaaacg ggcaagaacg ataaaataac cacaacgcat
     4861 agaataatca gcgaaaaaac aatcgaccag ggtgtctatt ttaccaccaa gggagacgcc
     4921 aataatactc cggacatgga ggagattccg agcgagaaca tactgggccg cgttgttttc
     4981 agcataagca agctgggata cattctggat tttataagaa ggcctataag tattcttatt
     5041 attgttataa ttccggccat ttttataatt tttgatgaaa ccaaaaagat aatcgaggag
     5101 gttggaaaaa tgaaggatgg caaaaataaa attatcaaga aaaaataaaa aaaaggaaga
     5161 ggaaactttt tgggcagact ttttgctggg atttgttccg gtgttcattc tatatgtcat
     5221 gatagccggc tctgtcatag tttcaaaaac aagagccata ctccaagaca atcagagttc
     5281 tatcgggagt atcatacaag ccggtattct tgatttttcc ctcaatcaac cctcttcttt
     5341 ctcttccgcg cctcttcaaa aaggagagtc tgtcggggta gatgtatcgc tcttaaatga
     5401 cggcaaccta aattttcaat atatagcctc aactacgatt atgggtggcg accaagactt
     5461 atgttcagcg ttgacactcg aggcctcttt gaatggcgcg accatatacg ataactccgt
     5521 ttccgagttt gccagccctc ttcaaaatta cgttaacccc tcagactggg gcttgtcggt
     5581 cactctaccg gatacagcct cggactcatt gcaaaacaag agttgcaatc tgcgctttgt
     5641 ttttagcggg tggcaaaatg gactttcttc gaatcaaggt tttaacaacc ttgaattcat
     5701 agacgtaact atcaactcgg gcgagtggat tatccctgaa ccgggagatc ttaatatgtc
     5761 tcctatcagc gattcttaca ttagacaaga tcgagccacg acaaattacg ggggcggcac
     5821 aacaatgctt gttcaatcgc gctccggctc gagaaactat cgtgcggtca ttagatttaa
     5881 ttttaagcta ccctccggag taataataga ttcttccttc ttgaaattgt atatgacatc
     5941 ggcaccaagt gcatccagaa attatgaggc gaggagtctt ctcggcagct ggacagaaac
     6001 cggtgttaac tggagcaacc agcctgcgtc cagcgcttct ccatcagata caataactac
     6061 cggcacaacc cgtaatttat ggatgtcttg gaatgtcaaa aacgatgttc agtcgattgt
     6121 gaatggtgcc gcaaattatg gatggctcat taaggatgtg acggaaaact ccagcatgac
     6181 acgtggcgcg acttttgctt caagagaaaa ctcaaccgtc ggcgtcagac ctgtcctaga
     6241 ggtggcgttc tccgccccac ccgcgtcaac agaccatctt gttgttaacg aagtttatta
     6301 tcaagttgct gtcggtaagg gtgtagaaac gacagatgaa tgggtcgaat tatacaaccc
     6361 caccacaagc gacatagatc ttctggggtg gaaaatttgc gataatggcg gttgcgacac
     6421 gctgggatca tacacgctac cttcacgaag ctttgcggtc gttaccccga atacaagtaa
     6481 ctttgataca tattggacac ttgctccggg tgcaattaag ataacattgc caaatacgaa
     6541 tatcggaggc aacggtcttg gaaacacggg tgatcgcata tatatacaaa atgcatcaag
     6601 cacggcagtg gacggaataa gctacgggac tgatgttagc caactcaatc cgccagtttc
     6661 cacgggcgga cttgggtctt ccctcgcgcg cattattaag ggatacgatt tcaatgttgc
     6721 cgatgactgg attaccaact ctactccgaa cccgggaact aatccggggt ccgacagtaa
     6781 agaaataatg agattcaccg attatggcac cgaggtggcc gcaagctcgg tcgggctcag
     6841 tccgctaata acaaactaca ttcccgcaga taccgatatg agcggagagg aagaaacccc
     6901 gacagagatt tttaaacaat gttcggcggg aagcatgccc gtcgcaaaag aagaagccac
     6961 attcacagaa aaaaccggcg ggacaatcga gcccgtggcc gcttcttcca caacaacaga
     7021 ccctattatc gagcccgaca tttccacgac aacacctgaa ataattacgg atattttgcc
     7081 ggccgaggcc acttcaacag atcagaccgt ggacacgaac acaccatctg agccggaaac
     7141 gacagaagcg ttaccgggca cttctcccgc agacacaaac gttatagtgg aattaccgac
     7201 ggcggaaacc gaaaccacac aagatgtttt aatagcggaa gaacaagcgc ccattgcaga
     7261 acccgctcaa gaaatagatc cagtcgagac gccaacagaa caataaaatg aaaattaata
     7321 ttcagacaaa tatagcgctc cgagtcatcc ttgccgtttt catgctcttt gccgtgcacg
     7381 caaaagccgc cgacccgacg gttttattcg aacaaactcc cctatttagc ggcactaatt
     7441 ttgctcccgg agagacagtt acaagatgga ttaaaataac aaacaatacc gacgaagcgc
     7501 atatgtctgt actgaaagcg gtaaatctgg ccagcgttaa agatcttggc tcccaaataa
     7561 aaatagagat aaacgaaggc ggatcaacaa tttactcggg aagtttttcc gatcttttca
     7621 atctctctga actgaaactt aaagaaatcg ggccgggaac acaaaagaaa ttcaatctta
     7681 cagcaacatt tttgcccgat tcaaaagatt catatcaagg aggttctctt agttttaata
     7741 taaaaacagg actggaagac gtaataatag tgtcggatgc tccacctgtc ggaggaggcg
     7801 gacctatggc gcccgttacg gaaaagatta cattttccga aatattttta gagcaaaata
     7861 acggagacga ttcactcaac gtaaaatgga acacaaatct tcccgcgacc agccgggttg
     7921 tatatggacc cgtctctatg ggcccctatt cagcaaatct caactctcaa aatatcggat
     7981 atctcttgtc tacaaaagaa aatcccctcc cacaaaatac acactctgta gagcttactg
     8041 gcctaatccc cggagaagaa tattatttta gaattatatc cgaggccggg atgtttaacg
     8101 caagccaaga atatgtatta aaattagaca aga
//
LOCUS       DAOTEX010000037         7765 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3118
            NODE_6645_length_7765_cov_4.946693, whole genome shotgun sequence.
ACCESSION   DAOTEX010000037 DAOTEX010000000
VERSION     DAOTEX010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868142
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 7765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 883
            CDSs (total)                      :: 833
            Genes (coding)                    :: 829
            CDSs (with protein)               :: 829
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7765
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6645_length_7765_cov_4.946693"
                     /isolate="AMR_MDS_3118"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(35..208)
                     /locus_tag="P5056_02875"
     CDS             complement(35..208)
                     /locus_tag="P5056_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62686.1"
                     /translation="MKKQAIEKGTKVEVLGKGEGVVKHVSFKDAEPFPIEVYAVELST
                     GETKHFTADELKA"
     gene            complement(219..545)
                     /locus_tag="P5056_02880"
     CDS             complement(219..545)
                     /locus_tag="P5056_02880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62687.1"
                     /translation="MRITIGTEAKQILAANPKRKRWELQMIPQSIEAGNTGYIYIGKG
                     YPPSTNSDSMAKDAILSDGASVGDNVDALLEKCPYTGAVWAVATIAAQVCTLFEENIT
                     ENGNSK"
     gene            complement(732..1595)
                     /locus_tag="P5056_02885"
     CDS             complement(732..1595)
                     /locus_tag="P5056_02885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62688.1"
                     /translation="MNKVSKIYDGTDPGATPGRVFTASIGQETIVQEGKFLDYLMFGL
                     KGAVSTAAVAIEDFAGVLSEYTLRVGADTRILLNLRQMVALMAFYYGELPFIWENTDN
                     TGNDFIGGVKLPLNLAVDANKPINHAATKTAVANVGTETLGISGYFSDAGNGKKPIHA
                     VAISKTTAAATGYDLADVTLPPIGKLKGVILQHANTFADGNIDISVQRLRLLVNGQIS
                     SQFNSLTDSEGIPGIFNGVEDPITDMLKVFSFFRLPGDGIDLKANEVKFQLDVEDASD
                     AVAIIPVIEIE"
     gene            complement(1616..2020)
                     /locus_tag="P5056_02890"
     CDS             complement(1616..2020)
                     /locus_tag="P5056_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62689.1"
                     /translation="MEVRSNATKWATRSAAAGSDYASGVQTPRRSWAAATAASEGNYE
                     AGVNAAIARKAYGKGISNAGDATWQKGVAEKGRTRYQQGVSVSSDNYSRGFQPFADTL
                     RSVTLAPRGPKGQNFGRVQQVGEALRAKKLQA"
     gene            complement(2630..3184)
                     /locus_tag="P5056_02895"
     CDS             complement(2630..3184)
                     /locus_tag="P5056_02895"
                     /inference="COORDINATES: protein motif:HMM:NF024605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRY62690.1"
                     /translation="MKVIGITGTNASGKDTAADYLKDKYGFKNYSLSDEIRFEATQRG
                     LPHERENLRLIGNELREKFAPNELAVRATRRITASGDNKVVVTSIRNPAEAEYLKNNF
                     PGFKLIYVDAPVEMRHERSRARGRVGDGVNLDDFRAAEAKELEGGKTGQQLLKCQDMA
                     DFEIINDGTLEKLFEKIEKVLSQI"
     gene            complement(3254..3577)
                     /locus_tag="P5056_02900"
     CDS             complement(3254..3577)
                     /locus_tag="P5056_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62691.1"
                     /translation="MKSANEYIREFNAFAKDNKDWALRMIFLGFLAEMMEAPCIADKN
                     LSAKEKEKRQKDFLSFLDGQDAKWKAISIATKGAVVKNGFKALLLKHFPHIFNNWRIR
                     KLLST"
     gene            complement(3660..4343)
                     /locus_tag="P5056_02905"
     CDS             complement(3660..4343)
                     /locus_tag="P5056_02905"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HRY62692.1"
                     /translation="MKAVILAAGRGIRLRPHTDQIPKPMLKVGGDPILFHTLSILPDE
                     VSEVFIIVGWLQDEIKNYFGKEFKGKKITYLSQNEPKGTFHALSLAKDFLDEPFLMIS
                     GDDIYSKKDISEVSKKESAVLVHKTQNPERFGICKRGHDGLLCEIIEKPKEFVGDLAN
                     IGVYKLKPTIFKEDIVWGGNGEQVLAPMIGNLAKKEKIWLTEASFWHPIGDISDLNKA
                     QTLDLTEVS"
     gene            complement(4340..4603)
                     /locus_tag="P5056_02910"
     CDS             complement(4340..4603)
                     /locus_tag="P5056_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HRY62693.1"
                     /translation="MFYYTYLLRSKKDGKWYTGVTNNLRKRFYQHNTGKNTSTKGRGP
                     FELIYYEASLNEEDAGSREVYLKSGVGKRYLKNRLKRFLSLTG"
     gene            complement(4659..5294)
                     /gene="trmD"
                     /locus_tag="P5056_02915"
     CDS             complement(4659..5294)
                     /gene="trmD"
                     /locus_tag="P5056_02915"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009019 - tRNA
                     (guanine-N1-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HRY62694.1"
                     /translation="MKTFSIITLFPEMIDSYINESILKRAQEKKLIKFKVLNPRDFTT
                     DKHHKADDRPYGGGPGMVMYAEPILKAVAKAKGSKPAKILIMSPRGKQFTNELAKKLV
                     TKNENIILISGRYEGIDSRVKKILKAEEISVGPFVVTGGELPALIIIDAMTRQIEGVL
                     GKSESVEESRVSSGEMYTRPEILKYKGKTYKVPKVYLSGHHKNIEERRKSH"
     gene            complement(5332..5793)
                     /locus_tag="P5056_02920"
     CDS             complement(5332..5793)
                     /locus_tag="P5056_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008584198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase family protein"
                     /protein_id="HRY62695.1"
                     /translation="MLGPRKDAISWDECFMRIARTIAERSKDPSTQAGAIIVTKENVP
                     VGLGYNGLPRGLKNEEFPWDREGDFLDVKYTYLCHAEENAIFNSNNTTKGCKIYATLF
                     PCNECAKSIVQNGITEVIYESDKYHDEKIWIASRKILDAAGVTYRQYSSKE"
     gene            complement(5923..6522)
                     /locus_tag="P5056_02925"
     CDS             complement(5923..6522)
                     /locus_tag="P5056_02925"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HRY62696.1"
                     /translation="MDKKFEWYIGIDEVGRGPLAGPITICAAAFRDRKPRALSGIKDS
                     KKLSGKQKGEWKKEIEKEKSSGRAVFCISSSDHLTIDDMGMTQATALAINEALDKLDL
                     DPEKCLVLLDGGLKAPEKFIYQETIIKGDEKERVISAASIIAKIDRDSLMDKYSGKYP
                     EYGFHLHKGYGTRAHYEALEKHGPCEIHRKSFLSSLRKQ"
     gene            6643..7158
                     /locus_tag="P5056_02930"
     CDS             6643..7158
                     /locus_tag="P5056_02930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62697.1"
                     /translation="MKTPTTEQWERAVDDILSGGMTNADFQKLLDMGVFRELGLAKVD
                     KIDMKRLHLALNPPEKKPRETIIYVGTLENVMEQGMERHGYGLRQDGYDTDGWDGVVL
                     FDDGDLLAIFDHAGKELHRVKVRMIHSPEGPLTAKGIPWRLWHSYFYPKDKVPFRAEL
                     TKFVKPKGKKK"
     gene            7340..>7765
                     /locus_tag="P5056_02935"
     CDS             7340..>7765
                     /locus_tag="P5056_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HRY62698.1"
                     /translation="MKKFVHLHTHTHYSLLDGLSKPKEIIKFAKQDEMPAIAITDHGN
                     LHGAIEFYQKAKKEGVKPIIGVEAYIANNTRFDKRPNVDNKRYHLTLLAKNKDGYQNI
                     VKMVTLSNLEGYYYKPRMDRELLRQYGKNVICLSGCLGGE"
BASE COUNT         1925 a         1771 c         1652 g         2417 t
ORIGIN      
        1 aagcttttcc ttgtttatct tcataagcag gggattaagc tttcaattcg tcggctgtga
       61 agtgtttcgt ctcgcctgtg gataactcca ccgcgtagac ttcgatagga aacggctcgg
      121 cgtccttgaa gcttacatgc ttcacgactc cttcgccctt gcccaaaact tcgactttag
      181 ttcctttttc gatagcttgt tttttcatgg ttttactttt atttcgagtt cccattttcg
      241 gttatgtttt cttcgaacag agtgcagacc tgagctgcga tcgtagcgac cgcccaaact
      301 gcgccggtat acggacactt ttcgagcaaa gcgtcgacgt tgtcgccgac agaagcgccg
      361 tcggacaata tggcgtcttt agccattgag tccgagttcg ttgatggggg ataacctttg
      421 ccgatgtaga tgtatccggt gttgccggct tctatcgact gaggtatcat ctgcaattcc
      481 cagcgctttc gcttcgggtt cgcggcgagt atctgttttg cctccgttcc aattgtaatt
      541 ctcatgttag acctttaaat taaatctccg cgataaatta tcgttacgca ctttctttaa
      601 gaagcacccg acacagtcgg ggtacgaact gaccgttttg ccgagcttct cgcccgaggg
      661 cgataacttt tcaaaaggtt gtccgcaccc cctgcagtgc gtgaacattt tggtgttctt
      721 ttcgggtttc attattcgat ctcgatgacc ggaatgatag cgacagcgtc ggaagcgtcc
      781 tcaacatcga gttggaactt gacttcgtta gctttcaaat cgattccgtc tccgggaagt
      841 ctgaagaatg agaagacctt gagcatgtct gtgataggat cttctactcc gttgaatatg
      901 cccggtatgc cctcagaatc ggtcaatgag ttgaattgag agctgatctg tccgtttacc
      961 aaaagcctta atctctgaac ggaaatgtca atatttccgt ctgcgaaagt attggcgtgc
     1021 tgtaagatga ctccctttag cttaccgata ggaggaagcg taacgtccgc gaggtcgtaa
     1081 cctgtagcgg cggcggttgt cttagaaata gcgacagcgt ggatcggctt cttgccattt
     1141 cctgcgtctg agaagtagcc ggatattccc aaggtttccg ttccgacgtt tgcaacggct
     1201 gtctttgtgg ctgcgtggtt gataggcttg tttgcgtcaa cagcgaggtt caacggaagt
     1261 tttacgcctc cgatgaagtc gttaccggtg ttgtcggtgt tctcccatat gaaaggaagc
     1321 tcgccatagt agaaagccat aagagcgacc atttgcctca agttgaggag tattcttgtg
     1381 tcagcgccta ctctcaaggt gtactcggag agaacgccgg caaaatcttc gatcgcaacg
     1441 gcggcggtgg aaacggcgcc cttaagaccg aacatcaaat aatctaggaa cttgccctct
     1501 tggactatag tttcctgtcc gattgaagcg gtgaaaactc gtcccggagt agcccccgga
     1561 tctgttccgt cgtaaatttt agatactttg ttcatgaaga taggggatta aactattaag
     1621 cttgaagttt ctttgctcta agagcttcgc cgacctgctg aactctgccg aagttctgac
     1681 cctttggacc gcgaggcgcc aaagtgacgg atctaagagt atcagcgaaa ggctgaaatc
     1741 ctcttgaata attatcagaa gaaacagaaa caccttgctg atatcttgtt cttccttttt
     1801 ctgcgacacc tttctgccat gtcgcgtcac ctgcgtttga aatacctttt ccgtaagctt
     1861 ttctagcgat agcggcgttt acaccggctt cgtagttgcc ctcggaggca gccgtagcgg
     1921 cagcccatga tctcctagga gtctgaactc ctgaagcgta atctgagcca gcggcagccg
     1981 atcttgttgc ccactttgtg gcgtttgatc taacttccat ttttttatta ttaaaaatta
     2041 tttattaaat ttttacttca atctatccac tggccagtga gatatttgag gctgtaattt
     2101 tcctcaaaga ggaatggcta agcgcaccct agtgacgaac taaaattttt tctagttagc
     2161 cactcctctt aaaagaaaat ttttatttat caacatactt atccacagtt tctacaaaaa
     2221 tttatatata tattaaatat ctatacacca atttcgtagc gaagcggaga aattggtggg
     2281 agaaaagccg gggggatatg caaggggggt tttccgaaaa agcccacgaa cgctctaaaa
     2341 gcgcctacga aaatatagtt catcgatcca accagtcggc gaaaatgtga gttccaattt
     2401 tctctgaacg tattcgtgtc ttccgagaaa agaggggatc ctataaagtc cgacaaagtt
     2461 gctgatatag ttgtgcgcaa catttgtact gctgacgacg tagggtagtg tgtgaagctt
     2521 tgataatgcg tcgcacaata aacattgtgc gactcactat ctattttaaa caccattctc
     2581 ccctttataa aaaacacccg ccgtgtgccg gcgggcgaaa attattttct tatatttgtg
     2641 acaaaacttt ttctatcttc tcaaaaagtt tttctaaagt tccgtcgtta atgatctcga
     2701 agtctgccat gtcttggcac ttaagtaact gctgccctgt tttgcctccc tcgagctctt
     2761 ttgcctcggc cgccctgaaa tcgtccaaat tgaccccatc acccacccta cccctcgcac
     2821 gagacctttc atgcctcatt tctactggag catccacgta gatcaattta aatccgggaa
     2881 aattattttt tagatactct gcttcggccg gatttctgat acttgtgaca acgaccttat
     2941 tgtcgccgct cgcagtgatt cttcttgtcg cccttacagc aagctcattt ggtgcaaatt
     3001 tttcccttag ctcattgcct ataagcctaa ggttttccct ctcgtgaggc agtcccctct
     3061 gcgtcgcctc aaaacgtatc tcatcggata gagaataatt cttgaagcca tatttatctt
     3121 ttagataatc cgccgcagtg tctttgccgg atgcattcgt tccggtgata ccaataactt
     3181 tcatttttag attttaacag ttttcatata aataaaaaag cagaccacga gagggtctgc
     3241 ttttgagtaa tgcttacgtc gaaagaagct ttctgattct ccagttatta aaaatgtgtg
     3301 gaaaatgctt taaaagaagc gccttaaagc cgttctttac gactgcgccc ttggttgcga
     3361 tagagattgc cttccatttt gcatcttgtc cgtcgaggaa agaaagaaaa tctttttgtc
     3421 ttttctcttt ttccttggca gagagattct tgtcggcgat gcagggggcc tccatcatct
     3481 cagccaaaaa tccgaggaag atcattcgaa gcgcccagtc tttgttgtct tttgcaaatg
     3541 cattaaactc gcgaatgtac tcatttgcgc ttttcatata ttacctcctg ttttggtctg
     3601 cgatcgtgtc gcgacgtatt ttgtgaaaag gacaaaaaat ctataaacaa tatatcttcc
     3661 taggacacct ctgtcaagtc gagggtttgg gctttattta gatcacttat atcccctatc
     3721 ggatgccaga aagaggcctc tgtgagccat attttttctt ttttagccaa gttgccgatc
     3781 atcggtgcca gaacctgctc tccatttcct ccccaaacga tatcctcctt aaagatggtg
     3841 ggttttaact tatatactcc gatattggca aggtcgccga caaattcttt cggcttttct
     3901 attatttcac agagaagtcc gtcgtgtccc cttttacaga ttccaaacct ctccggattc
     3961 tgagttttgt gcacaaggac cgctgattct ttttttgaaa cttcgcttat atcttttttt
     4021 gaatagatat cgtcgccgga tatcatcaag aacggctcgt ctagaaaatc tttagcgagg
     4081 cttaacgcgt ggaatgtccc tttcggttcg ttctgcgaca gatatgttat tttcttgccc
     4141 ttaaattctt ttccaaaata atttttgatc tcatcctgaa gccagccgac aatgataaag
     4201 acctccgaaa cctcgtcggg taatatagat aatgtgtgaa aaaggatagg atccccaccc
     4261 acctttagca tgggcttggg tatctggtct gtgtgcggac ggagccttat tccccttccg
     4321 gcagctaaaa taacagcttt catcccgtta gagataggaa gcgcttcaat ctattcttta
     4381 gataacgctt gcccacccca gattttaaat aaacttccct cgacccagcg tcttcttcat
     4441 ttaaagatgc ttcgtaatat atcaattcaa atggaccccg gcctttcgtg gaagtattct
     4501 ttcctgtgtt gtgctgataa aagcgcttcc gtaaattatt tgtgacccca gtataccact
     4561 taccgtcctt cttgcttctt aataaatatg tataataaaa cattttaatc tctaacggga
     4621 tttcatctta ctgttactat aacagatttt tagaatattt agtgactctt gcgcctctct
     4681 tcaatatttt tgtgatggcc cgacaagtat acctttggca ctttatatgt tttgccttta
     4741 tattttagaa tttccggtct ggtatacatt tctccgcttg acacacgaga ctcttcaact
     4801 gattctgatt taccgagcac gccttctatc tgtcttgtca tcgcgtctat tattataagt
     4861 gccggcagct cccctcctgt cacgacgaat ggcccaacgg agatctcctc cgctttaagt
     4921 atttttttaa cacgagaatc tatcccttca tatcttcctg atatgagtat tatattttcg
     4981 ttctttgtga ccagcttctt ggccaattcg tttgtgaatt gtttaccacg tggagacatt
     5041 atcaggattt ttgccggttt gctccctttg gctttggcaa ccgctttaag tatcggctcg
     5101 gcatacatta ccattcccgg tcctccgcca taaggtctgt cgtcggcttt gtggtgcttg
     5161 tcggtcgtaa agtcacgcgg atttaaaacc ttgaatttta ttagtttttt ttcttgcgcg
     5221 cgcttcaaaa tagattcatt tatatatgaa tctatcattt ccggaaaaag cgtaataatg
     5281 ctgaacgttt tcattcagcg cattatatct aaataaatag ataacgtcaa actactcttt
     5341 tgaagaatat tgtctgtatg tcactcccgc ggcgtctaaa atttttcttg aggcaatcca
     5401 aattttttcg tcgtgatatt tatcgctctc gtatattacc tctgttattc cgttctggac
     5461 aatagacttt gcacattcgt tgcaaggaaa gagcgttgca tatattttgc atcccttggt
     5521 tgtattgttt gaattaaaga tggcattttc ctcggcgtgg cacaaataag tgtatttcac
     5581 atccagaaaa tcaccctctc tatcccatgg aaattcctca ttctttagcc cgcgaggaag
     5641 gccgttatag ccgagaccca caggcacatt ctcttttgtg acaataatgg ctcccgcttg
     5701 cgtgctcggg tcttttgagc gttctgctat ggttcttgca atcctcataa aacactcatc
     5761 ccatgatatt gcatcttttc ttggtcctag cataaaaaat tacaaattaa taattactaa
     5821 ttacgaattg attataacaa atatctttaa tggtgcgcta tttcattgag gtggatagaa
     5881 ggtagaaagt agagagtagg acgtgtagga aaatagaaaa agttattgtt ttcttagtga
     5941 gcttaaaaaa ctctttcggt gtatctcgca ggggccgtgt ttttccaggg cttcataatg
     6001 ggcccgtgtg ccatagccct tgtgaaggtg gaacccatac tcaggatatt ttcccgaata
     6061 tttatccatt aaagaatccc tgtctatttt tgcgattatt gaggcggccg atattacgcg
     6121 ctccttctcg tcccctttta ttattgtctc ttgatagata aatttttccg gcgcttttag
     6181 cccgccgtct aggagcacaa ggcacttttc cgggtcgaga tctagcttgt cgagcgcctc
     6241 atttatggcg agggccgtcg cttgtgtcat gcccatgtcg tcgatggtca gatgatccga
     6301 tgaagatatg caaaacaccg ctcttccgga gctcttttct ttttctatct cttttttcca
     6361 ctcccctttc tgcttgcccg aaagtttttt tgagtctttg atgccggata gcgcgcgcgg
     6421 cttcctgtcg cgaaatgctg cggcgcatat agttatcggc cccgcaagcg gccctctccc
     6481 gacctcgtct atgcctatat accattcaaa ttttttatcc attttgtgta tacccaatta
     6541 tcttattttt agtcagaaag ttaaatagac agttgcccgt ttgagtggta gtatggcgac
     6601 gggttgggac tttgaaaaaa taaaatataa ggaggttctc ttatgaaaac gcccacaact
     6661 gaacaatggg agcgcgctgt tgatgatatt ttgagcggcg gtatgacgaa tgccgatttt
     6721 cagaaacttt tggacatggg agtattccgc gagcttggac ttgccaaggt cgacaagatt
     6781 gatatgaaga ggcttcatct ggcccttaat ccgcctgaga agaagcccag agagactatc
     6841 atttacgttg gcacgcttga gaatgtgatg gagcaaggta tggaacgcca cggatacggt
     6901 ttgaggcagg atggctacga tactgatggt tgggacgggg tagttctctt cgacgatggc
     6961 gacttattgg ccatttttga tcacgccggc aaggagttgc acagggttaa ggtcaggatg
     7021 atccattctc cggaaggtcc tcttacggcg aaaggtatcc cctggcgttt gtggcacagt
     7081 tacttctatc ctaaagacaa agttccgttt cgcgccgagc tgacgaagtt cgtcaagccc
     7141 aagggaaaga aaaagtagca tatgcagtta gcgcttcttg aaaaagaggc gcttttttgt
     7201 ttgggtcatt gcgagcggag cgaagcaatc cagatttaat tggaccaaca cctagattgc
     7261 ttcgccctgc tcgcaatgac gattaatttt gatatactaa gttaatagtt cgtaattctt
     7321 aatttttaat tcgtaattca tgaagaagtt tgttcaccta catacacaca cccactactc
     7381 ccttctcgat ggtctttcga agccgaagga gataatcaaa ttcgccaaac aagacgagat
     7441 gcctgcaatt gcaattactg accacggaaa tttacatgga gctatcgaat tttatcaaaa
     7501 agcaaaaaaa gaaggggtaa agccgattat tggggttgag gcgtatattg caaacaacac
     7561 tcgttttgat aagcgcccga acgtggacaa caagagatac caccttacat tactcgcaaa
     7621 aaataaagac ggataccaaa acattgttaa aatggtgacc ctctcaaacc tcgagggtta
     7681 ttattacaaa cccagaatgg acagggagct tcttcgtcaa tatgggaaga acgtgatctg
     7741 cttgtcgggg tgcctcggcg gagaa
//
LOCUS       DAOTEX010000038         7626 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3118
            NODE_6806_length_7626_cov_4.249637, whole genome shotgun sequence.
ACCESSION   DAOTEX010000038 DAOTEX010000000
VERSION     DAOTEX010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868142
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 7626)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7626)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 883
            CDSs (total)                      :: 833
            Genes (coding)                    :: 829
            CDSs (with protein)               :: 829
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7626
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6806_length_7626_cov_4.249637"
                     /isolate="AMR_MDS_3118"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(111..359)
                     /locus_tag="P5056_02940"
     CDS             complement(111..359)
                     /locus_tag="P5056_02940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62699.1"
                     /translation="MKEDNDTSQPIFILGVILITFFVGHGLYNPACPILSVLGVTFLS
                     LIMSVIWVSLLVLCSIWCMEWEKELVLEAQRIAENEED"
     gene            complement(646..1017)
                     /locus_tag="P5056_02945"
     CDS             complement(646..1017)
                     /locus_tag="P5056_02945"
                     /inference="COORDINATES: protein motif:HMM:NF013214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1 family protein"
                     /protein_id="HRY62700.1"
                     /translation="MLNIYSAGLIAKAFGMTALITFLMGFLGIAYPDAFKNMGGLLFF
                     ALGGLIIVRIMQIFIPALAALTIVDYIGAGIFSLYIAYDMYRASKVPRTLDNAVDISV
                     SLYLDIINLFVTLLQIYANED"
     gene            complement(1033..1314)
                     /locus_tag="P5056_02950"
     CDS             complement(1033..1314)
                     /locus_tag="P5056_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62701.1"
                     /translation="MKSFSCSNGVFERYGANSLSERAFYLVLTTCVLWGLIATATAAS
                     YAASIGYYPSGAGILFVGFIIPFLGIYLSVTSDNPLVSFVGYNMITIPF"
     gene            complement(1337..1576)
                     /locus_tag="P5056_02955"
     CDS             complement(1337..1576)
                     /locus_tag="P5056_02955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62702.1"
                     /translation="MAPELIPATQLPTIKLVVTKSANSKKNSEEISEEEQRKKDLERY
                     GDVPVNELEWAVEAELEEMKKPKKPAGPKTQIFYE"
     gene            complement(1849..1989)
                     /locus_tag="P5056_02960"
     CDS             complement(1849..1989)
                     /locus_tag="P5056_02960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62703.1"
                     /translation="MNLLDAFLDELSDSQFLIAAIAIALAYEMRNYIPIIAVSAYAYV
                     VS"
     gene            complement(2016..2690)
                     /locus_tag="P5056_02965"
     CDS             complement(2016..2690)
                     /locus_tag="P5056_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62704.1"
                     /translation="MDKKLFAAIVAIVCLAGLAVIVIAVLAFCPARANANETKDIAYG
                     AIASFVVHEAAHGFAAGNDEMTLHGPITNPKWEYKGRSGTYKTVYDKKTGTWVREYLD
                     IDRYKKNLEGVSKAGMIGNDAVSHWTMAKNLRGKKRFYNMRKGFVAWNILNNAYYLTA
                     SKDRGDLDPANFRSEKHRERFKKIITVDTALLTYEFFAKRSIFPKGTSLSFKRRTFMF
                     SKSWGF"
     gene            2779..2961
                     /locus_tag="P5056_02970"
     CDS             2779..2961
                     /locus_tag="P5056_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62705.1"
                     /translation="MRGLPRLLLRSGPDGLFPPERFGAEVVVVRGNLVEDGEVVEDVV
                     GVSLHQGTLLLELGPF"
     gene            complement(3126..3581)
                     /locus_tag="P5056_02975"
     CDS             complement(3126..3581)
                     /locus_tag="P5056_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62706.1"
                     /translation="MEINPVINRLKVLSVVIGSFFLFLFFSINNWSTYDLGLVIEQTA
                     FIIFFASKIIRRNYKKNIDDTLLAFVAIVAFAGMLFGLVTELPLLECVALAVCPVLSG
                     LLFGVATPYPESDDREAYEHFISYAVLQVCFVLMFGSFSHYSGGWVFWY"
     gene            complement(3814..4182)
                     /locus_tag="P5056_02980"
     CDS             complement(3814..4182)
                     /locus_tag="P5056_02980"
                     /inference="COORDINATES: protein motif:HMM:NF021567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2293 domain-containing protein"
                     /protein_id="HRY62707.1"
                     /translation="MSKKIPKRYRERARQLFNEIRRQFPSCQDPWPIIAFALNNKNSV
                     VFDGTVPVAEQAELIVKGRVRHVDTNYEKLLSQGIEREEARRAICREQNKKLCELRAA
                     KEDDGLYMRDPKTTLDSFRS"
     gene            complement(4335..4487)
                     /locus_tag="P5056_02985"
     CDS             complement(4335..4487)
                     /locus_tag="P5056_02985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62708.1"
                     /translation="MEQNENAPLSKVEVCSLVILFLTVGLGTTYGVYLFAQACNEAMI
                     RYSFTF"
     gene            complement(4515..4970)
                     /locus_tag="P5056_02990"
     CDS             complement(4515..4970)
                     /locus_tag="P5056_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62709.1"
                     /translation="MGNNDIVAAITVIIVILGICAGNVSVVIDGVTTLFCTLGLIFSA
                     VAIEVLEDNSKHYKYPLSPETVSGALGSCFLLALLFLASGKIMVVTFLRLSIVAHLTT
                     GIVALGMRCVYDMTNERLTLSSRKGSLFVMTLAIFGWAYTVGSVIKAML"
     gene            complement(5108..5449)
                     /locus_tag="P5056_02995"
     CDS             complement(5108..5449)
                     /locus_tag="P5056_02995"
                     /inference="COORDINATES: protein motif:HMM:NF021567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2293 domain-containing protein"
                     /protein_id="HRY62710.1"
                     /translation="MKEQGKAQELLSEASRQFPSCPNAKEIVYAAVNNKRSAAHDETL
                     SNYEKIDRIFKAWIRHDFTDYEERIARGTNRTQARTDTWPKVASIIRQWQQRKQEGSS
                     NESKGSLCSHS"
     gene            5783..6490
                     /gene="tmk"
                     /locus_tag="P5056_03000"
     CDS             5783..6490
                     /gene="tmk"
                     /locus_tag="P5056_03000"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HRY62711.1"
                     /translation="MTTKNNKKRGKFIAIEGPDGAGKSRAIEFVKKELMELGFDFVFT
                     REPGGTITADAIRAIVKDPAYADMSVPCELGLFSSARADHLEKVIIPALEAGRNVFTD
                     RFSGSTYVYQVEVAGEGKYEEFFAKTNKLFVGDYEPDMWILLDVTSEEAQKRMQKRRQ
                     MELEEAKLNGGALEKDKNDAKELDFHKKIRKGYHKFFKKFVKGKKGKLIDTTKLTEEE
                     VGQAVLKTILNFLHPVE"
     gene            complement(6746..>7626)
                     /locus_tag="P5056_03005"
     CDS             complement(6746..>7626)
                     /locus_tag="P5056_03005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62712.1"
                     /translation="LFAMRPPAVAPAPAQSGQDLDESSNSTTAEVTAEDFQDEATVEN
                     TVQTTGEVKADPNAEIRKLVLEKLKAANTSEEFHELWVATQNISDLNNVVEKTWDEFF
                     LDKAKQTNDPKELRGLILDAPLCGETHRYASRKISELKMKKLMEENGEPSSTTTEVTA
                     APPEKEATNETSVQDTEEAALPGDEIILDKERESFNTAETILLGLARINESNIYILTA
                     SSILDSRRFDIIIKAQESKKMKDLVERYNEIFNQYLDSEEFSTLGELRKAVKHRAKMR
                     EDMVVVVNEILKILNK"
BASE COUNT         1863 a         1727 c         1966 g         2070 t
ORIGIN      
        1 gcgccaatac agtttttaac atcaaagtgt ttccaagcta ttgattagaa aacggggtca
       61 atatacgccc caaaataagg aaaaaccgcc tattttaagg cggtttattt ctaatcttct
      121 tcattttctg cgattctttg tgcttccaaa accagctctt tttcccattc catgcaccaa
      181 atagagcaaa gcaccaagag gctgacccaa ataaccgaca ttattaggct taagaatgtg
      241 actcccaaga cagataatat cgggcacgcg ggattgtaga ggccatgtcc cacaaaaaag
      301 gtaattagaa tgacccctaa aatgaatatc ggctgagatg tgtcgttgtc ctctttcatc
      361 ttgctccccc aattttttat gaagtatcag gaacccgtta ggtaccataa cgaaagcacg
      421 gagtagaaga cacagatcat aaaagcgaaa ataacgtcgc cccaacaggc gtaaattatt
      481 atcggtaatc ccaagacgag aaacaggaca tatttcttat aaaaattcat tgcccctcct
      541 tttccggtca gataaaattt tcaatgtcct taattattta tacgataata tacctttttg
      601 agaaaaataa aacccccgtc attgctgatg ggggatcgta ggaaattaat cttcgttcgc
      661 gtagatttga agcagggtta cgaacaggtt gatgatatcg aggtaaagag acaccgaaat
      721 gtctaccgcg ttatcaagcg tcctcggaac cttgctcgcc ctgtacatgt cgtatgcgat
      781 gtagagagag aagatgcctg ccccgatgta gtccacgatg gtcagtgcgg cgagtgcggg
      841 aatgaagatt tgcatgatcc gaacgatgat gaggccaccg agggcaaaga agaggagacc
      901 tcccatgttc ttgaaagcat cgggataggc gattcccaga aagcccatta aaaaggtaat
      961 gagagctgtc atgccgaacg ccttcgcgat gagtcccgcg ctgtaaatgt tgagcaacgg
     1021 cccgatcaga aatcagaacg gaatcgtgat catgttgtag ccgacgaacg agacaagggg
     1081 attgtcgctt gtcaccgaga gatagattcc caagaaggga atgatgaacc ctacgaaaag
     1141 gatgccggcg ccgctcggat aatagccgat ggatgccgcg tagctggcgg cggtagccgt
     1201 cgcgatgaga ccccacagga cgcaggtcgt gagcactaaa tagaaggcac gctcgctcaa
     1261 gctattcgca ccataacgct caaatacccc gttactgcag gagaaacttt tcattcacat
     1321 acccccttca ggtggtttat tcgtagaaga tttgcgtttt gggacccgcg ggcttcttgg
     1381 gctttttcat ttcttccaat tccgcctcaa cagcccactc aagttcgttc acgggcacgt
     1441 caccatagcg ctccaagtct tttttgcgct gttcttcttc ggagatttct tcgctattct
     1501 ttttagaatt tgcggatttg gtaacaacta gtttgatagt gggtaattgg gtggccggaa
     1561 ttaattctgg tgccattttt tgagggcgtc tacagcaaac acttttcgaa attacacggt
     1621 caatgttttg gtcaactcga cccatttcgt gtcttaaagc cgccacgaag agagtaaaaa
     1681 tgaaactaag caaaatggta ctgaatatac ccataagacc tcctttggag tcgttgatta
     1741 atttccttat tagattgtca aaggggctat ctcttggcag ataatacatt attttataca
     1801 aaagtaaacc cccatcattg ctgatggggg gggtggcttt cgccctagtt aagacacgac
     1861 gtaagcatac gcgctgactg cgatgatggg gatgtagttc cgcatctcgt aggcgagcgc
     1921 gatggcgatg gcggcgatga ggaactggct gtcggaaagt tcgtcgagga aggcatcgag
     1981 aaggttcatc gttttcctgc tttcttttgc cggcgttaga aaccccagct cttcgagaac
     2041 atgaacgttc tgcgcttgaa actgagactc gtgcccttcg ggaagatgga gcgtttcgcg
     2101 aagaattcgt atgtgagaag cgctgtgtcc acggtgataa tctttttgaa ccgctcgcga
     2161 tgcttctcgc tccggaagtt tgccggatcg agatcaccgc ggtctttcga ggcggtgagg
     2221 tagtaggcgt tgttcaggat attccacgcg acgaacccct tgcgcatgtt gtagaacctc
     2281 tttttcccgc ggaggttttt cgccatggtc cagtgggaga cggcgtcgtt cccgatcatt
     2341 ccggccttcg agacaccttc caggtttttc ttatacctgt cgatgtcgag gtattcccgg
     2401 acccacgtgc ccgttttctt gtcgtagacg gtcttgtagg tccccgaacg ccccttgtat
     2461 tcccatttgg gattcgttat ggggccgtgc agggtcatct cgtcgttgcc cgccgcgaat
     2521 ccgtgtgcgg cttcgtggac gacgaacgag gcgatggcgc cgtaggcgat gtccttcgtt
     2581 tcgttcgcgt tggcgcgggc ggggcagaag gcgaggacgg cgatgacgat gacggcgagc
     2641 cccgcgaggc agacgatggc gacgatggcg gcgaaaagtt tcttgtccat ttttcctcct
     2701 tggaaatttc ggttggttat cgtgatgttt tagggactaa cggaagatgt agcgaccgcc
     2761 gtacggcgcc cgtcgcgtgt gcgtggactt ccgaggcttc tgcttcggtc ggggccggat
     2821 ggccttttcc ctccagagcg ctttggtgcg gaagtggtcg tcgtccgagg aaatcttgtt
     2881 gaggatggag aggtagtgga agatgttgtc ggtgtaagtc tgcatcaagg aaccctcctt
     2941 ttggaattag ggccttttta ggcgttttta agttgtaaaa ggacgctatt taagtttctt
     3001 taattataca ccttttaggt aaaatgtcaa gcaaaataag gattcatcct gttgattaaa
     3061 aaacgccccc gaatcgtttc caattcgggg gccggcgttt tatctgccgc gatttttcta
     3121 ctttatcaat accagaagac ccatcctccc gaatagtggg aaaacgaccc aaacattaag
     3181 acaaaacata cctggagcac ggcataagag atgaagtgct cataggcctc cctatcgtcc
     3241 gattcggggt agggtgtggc tactccgaag agaagtccgg acagcacagg gcagacagcg
     3301 agagcaacgc attcaagcag gggcaattct gtaacgagtc cgaagagcat tccggcgaaa
     3361 gccactattg cgacgaatgc gagcagggtg tcgtcaatgt ttttcttgta attccttctt
     3421 atgattttgc tcgcgaagaa gatgatgaag gcggtctgct cgataacgag tccgaggtcg
     3481 tatgtgctcc agttgtttat tgaaaaaaat aaaaagagaa aaaaggatcc gataacaacc
     3541 gacagcactt tcagtcgatt aataacgggg ttaatctcca tctttcaatc cctccctggc
     3601 ccgccaaaac ttgtcacggt tctgacgata cggctagata tttgtgatat ctagattaac
     3661 ttttagtgcc tctttatttt taagttcaaa agtttaggag ggcttgcttc cggatcaaat
     3721 ttaaaagttc agtatcttcc atatttttaa cacaatgagg tgcgtttaac aatcccgtta
     3781 agtaaaaagc ccccgatctt tcgggggcca cacttaactt cgaaaactat cgagtgttgt
     3841 tttggggtct ctcatatata gaccatcgtc ttctttggcg gctcgtagtt cacaaagctt
     3901 tttgttttgt tctctgcaaa tagctcttcg ggcctcttct ctttcgatgc cctgactcag
     3961 cagtttctca tagtttgtgt ctacgtgtct aacgcggcct ttcacaatca gttccgcctg
     4021 ttctgcgacc ggaactgttc catcgaaaac cacggaattt ttgttgttaa gcgcaaacgc
     4081 gataatgggc caagggtctt ggcatgaggg aaattgtcgc ctgatctcat taaaaagttg
     4141 tctcgcgcgc tctcgatatc ttttcgggat ctttttggac aaacagcatt cctcccttgt
     4201 cgctaaataa acagcggagt cgttttttaa ggaacaaaat ttacagatga gaatagcata
     4261 tctaaatttt tatgtaaaat tttttatata ataaaacccc ctccgcttga ggaagggggt
     4321 tgctgtctgc gaacttagaa ggtgaaggag tatcggatca tcgcttcgtt acaggcttgg
     4381 gcgaataagt agacgccgta ggtggtgccg agcccgaccg tcagaaaaag gatgaccaag
     4441 gagcagactt ccactttgct gaggggggcg ttttcgtttt gttccatgag agtgcctcct
     4501 ttttttctag ttgcttacag catcgctttg atcacggagc cgaccgtgta ggcccacccg
     4561 aagatcgcga gggtcatgac gaagagcgat cccttgcgcg acgaaagggt gagtctctcg
     4621 ttcgtcatgt cgtacacgca gcgcatgcct agcgccacga tgccggtggt gaggtgagcg
     4681 acgatggaga ggcgcaggaa ggtgacgacc atgatcttgc cggacgcgag gaagaggagc
     4741 gcgaggagga agcaggagcc gagagcgccc gacaccgttt cggggctcag gggatacttg
     4801 tagtgcttgc tgttgtcttc gagaacctcg atggcgacgg cagagaaaat gaggcctaag
     4861 gtgcagaaga gggtggtcac tccgtcgatg accacggaga cgttgccggc gcagatgcca
     4921 agaatgacga tgatgacggt gatggcggcc acgatgtcat tgtttcccat tttgctgaga
     4981 cctccttagg tcttcgaggg acaccttgtg aaagggtctc gagttctttt agaagagctg
     5041 aaggccgccg aggacgatga acgagacgag catgccgatt gcggcgaagt cgtcggtcag
     5101 gttcgcgtta cgaatgcgag cagagtgacc ctttcgattc attggaactt ccttcctgtt
     5161 tccgctgttg ccactggcgg atgatggagg cgactttcgg ccacgtgtcg gtgcgagcct
     5221 gggttctgtt tgtgccccgg gcgatgcgtt cttcgtagtc cgtgaagtcg tggcgtatcc
     5281 acgccttgaa gatgcggtcg atcttttcgt agttcgacag ggtttcgtcg tgggcggccg
     5341 aacgcttgtt gttgaccgcg gcgtagacga tttccttggc gtttggacac gaagggaact
     5401 gcctcgatgc ttcgctgaga agttcctgtg cttttccctg ttccttcatg ggtacgttga
     5461 gttgcctcaa taccaccact ccttttagcg gtttctactg gtaaggacta tcgtgatgat
     5521 ggcatccatg atgacgccga caacgattag gcaggcgccg atcagagact ccatccgcta
     5581 ctcctttccc tgattttcaa tgtcctgacg ttttttaact ttctctgtat attatatacc
     5641 cattttgcct atttgtcaag caatgctaaa atatggctca ggatatagct acttaggaac
     5701 ccgtctacta agtcggggga gtgtttccct aataaatggc gataatgtaa aatggctata
     5761 aagaattttt aatattatag gtatgactac aaaaaataac aaaaagcggg gcaaattcat
     5821 tgctatagag ggtcctgacg gggccggcaa gagtcgagcc atagaatttg tgaaaaaaga
     5881 gcttatggag cttggttttg actttgtttt taccagagaa ccgggcggca ccataacagc
     5941 cgatgcgata agggccattg tgaaagatcc cgcgtatgcc gacatgtccg tcccgtgcga
     6001 gcttggcctt ttctcgtccg cgcgcgccga tcatttggag aaagttataa ttccggcgct
     6061 tgaagcgggc agaaatgttt tcaccgaccg tttctccggc tcaacttatg tttatcaggt
     6121 ggaggtagcg ggggagggta agtatgaaga atttttcgca aagacgaaca agctttttgt
     6181 cggcgattac gagcctgata tgtggatact tttggatgtg acttccgaag aggcgcaaaa
     6241 gagaatgcaa aagaggcgac agatggagct cgaggaggcg aagttaaacg gcggcgcgct
     6301 agagaaagat aaaaacgatg cgaaagagtt ggattttcac aaaaagataa gaaaaggtta
     6361 tcacaaattc ttcaaaaaat tcgtaaaagg caaaaagggc aagctgatag acacgacaaa
     6421 actcaccgaa gaagaagtcg gtcaggctgt attgaaaacg atattaaatt ttcttcaccc
     6481 tgttgagtaa aattttattt ataaaattta aaatttctct ttataaaaga gaaattttta
     6541 ctcaacaggg taaataaccc cccatttttc tttgtcatcc ccgcgcaggc ggggatccag
     6601 ttttatttta ttttagattg ccatggattc ccgccggagt ttaccctcga ctgcaatcgg
     6661 gggcgggaat gacaatcgag agatggttga aggcaagata aagaataaaa aaggcccgca
     6721 cattgtgcgg gccgtttggc tacatctatt tgttgagaat cttgaggatc tcgttgacca
     6781 cgacgaccat atcttctctc attttggctc ggtgctttac tgcctttctc aattccccga
     6841 gcgtgctgaa ttcttcacta tccagatatt ggttgaagat ttcgttgtat ctttctacga
     6901 ggtctttcat cttcttgctt tcttgggctt taataataat gtcgaagcga cgcgaatcaa
     6961 gtattgagct tgccgtcaaa atataaatgt tgctttcatt gattctggcc agacccaaaa
     7021 gaatcgtctc ggcggtattg aaactctctc tttctttgtc gaggataatc tcgtcgccgg
     7081 ggagggccgc ttcctcggta tcttgcaccg aagtctcgtt agtggcttct ttttcaggcg
     7141 gcgcggccgt cacttcggtc gtggtagagg atggttcccc gttctcttcc atcagtttct
     7201 tcatttttaa ttcactaatc tttcgggaag catatctatg agtttctccg cagagtgggg
     7261 cgtccaagat aagaccgcgc aactccttag ggtcgtttgt ctgtttggcc ttatcgagga
     7321 agaactcatc ccatgttttt tcaacgacat tattaagatc agatatgttt tgggtggcta
     7381 cccatagttc gtgaaattct tcagacgtat tcgctgcttt gagcttctca aggactagct
     7441 tgcggatctc agcgttcggg tcagccttca cttctccggt cgtctgcacg gtattctcta
     7501 ccgtggcctc atcctggaag tcctcggccg tcacttcggc ggtcgtagaa ttgctcgatt
     7561 cgtcgagatc ttgcccgctc tgtgcgggtg ctggggccac ggcaggggga cgcatggcga
     7621 agaggg
//
LOCUS       DAOTEX010000039         7604 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3118
            NODE_6843_length_7604_cov_4.246920, whole genome shotgun sequence.
ACCESSION   DAOTEX010000039 DAOTEX010000000
VERSION     DAOTEX010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868142
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 7604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 883
            CDSs (total)                      :: 833
            Genes (coding)                    :: 829
            CDSs (with protein)               :: 829
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7604
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6843_length_7604_cov_4.246920"
                     /isolate="AMR_MDS_3118"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(430..1362)
                     /locus_tag="P5056_03010"
     CDS             complement(430..1362)
                     /locus_tag="P5056_03010"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRY62713.1"
                     /translation="MDNIYDTIIVGFGPAGFAAALYAGRFAMNTLVVGKEFGGYAAKA
                     GTIHNYPGVKNADGYELSKTMKEQAEEFGAKFYDGEVLSIEKKDELFLVSTKDKKSFT
                     AKTIILATGSERRKMGLPRESELENRGIHYCVTCDGPIYVGKDIAVVGGGNAGVSAAL
                     LASEYAKKVYLIVKGDSLTAEPISTEKIKIMRDKIEILYKTEIKEFLGADSLSGVVLN
                     KDFNGSNKLKIDGLFVEIGSLPVNKLAKDIGVFLDDHGYARTDFSMKTNIAGAYVAGD
                     LCNAFGSFKQNITAAASGAVAANSAYYYLRSLNK"
     gene            complement(1363..1596)
                     /locus_tag="P5056_03015"
     CDS             complement(1363..1596)
                     /locus_tag="P5056_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002835306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HRY62714.1"
                     /translation="MKSVVIYSTNSCMYCRMAKDFFRENKVEYTEKDVYLDEKARNEM
                     VKMTGQSGVPVIKIGDEVIIGFDKATIKELLGL"
     gene            complement(1637..3313)
                     /locus_tag="P5056_03020"
     CDS             complement(1637..3313)
                     /locus_tag="P5056_03020"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRY62715.1"
                     /translation="MNISEAYLVSTPFPIIISTLSVVFNLLLGLVVFLNNRKSATNVI
                     FAFLNIFLAFFLASNLLSTQPSDYSEKLMWVRLVMFTAIFLNAFVYLFIRAFPHDELK
                     ISKKGGIFVAIVTIITALMTLTPFLITGISVPKTNVPVPIFGSPLSVFGKTINVGIAW
                     FSVFTFCFTLASVWALARKIISEKDPISRIQIKYLLAGVGIMFSAFFLFNLVFVAVFS
                     NTFFINLTPLAVSVFIMLTSYAILKHHLFSVKIIATELLVFSLWTFILIRTILSMTVE
                     DQMMNAGLLFLMVVTGIFLIRSVIKEVDQREKLAESAVALKESHEREMEKARTEAKLR
                     DEFVFVAAHELRTPITAIRGFLELVGDVEVNFPKDVQDDLGAIRMASDHLNELVNNLL
                     EIARSDAGAMKIETSPMLIAPVISEILKELGSLALEKEIKIIFNDSDNCNKILADDKK
                     TKEVFVNLIGNAIKYNKNNGTVNIDIDCIDEKNVVIKVKDTGFGIPKDQQGKIFGKFF
                     RASNKDTQGILGTGLGLFITKMLVEKMGGTIMFSSEEGVGTTFAVSFPRA"
     gene            complement(3323..4504)
                     /locus_tag="P5056_03025"
     CDS             complement(3323..4504)
                     /locus_tag="P5056_03025"
                     /inference="COORDINATES: protein motif:HMM:NF013094.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HRY62716.1"
                     /translation="MSLDKILSGKSREGVVSEPIIFDLKKEKDSKELSKLLKNKKIDR
                     VSDDYVEQLREFFAVSNPSLVYHPDFQKHFDEFLDGIKKKTSLSKHGKWAYFPWASCL
                     THILDDKSYQLVRTARNRNLINADEQDRFYNATIGIAGLSVGNSVALGIVMQGGAKHI
                     KIADHDSLALSNMNRIRAGAQDLGSLKVGMAARQIYEMNPYAKVEIFPDGITKKNIDK
                     FFNGLDIVVDEIDNIAVKYLIREQAKKRKIAVVMAADNGDNAVVDVERYDEKPQPKFF
                     HGRIGDVNYEMLTKLDKFGIGKTITKHVGPENVTERMRQSLLEMGKTIVSWPQLGGAA
                     MINGAAVAYCVRKILNGQTIISNRSLISMDEMLIPGYNSPSEIAKREEMIREFKKIFG
                     L"
     gene            complement(4591..6102)
                     /locus_tag="P5056_03030"
     CDS             complement(4591..6102)
                     /locus_tag="P5056_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62717.1"
                     /translation="MNIFSRDASFKKIKEYQKENNSSAWKKIGDKKTLDLFRAASVRV
                     PAYKDFLKKNKIDPGKIRSFKDFQLVPPMTKADYLRKYPLEKLCFDGTLKNKSTVFTS
                     TSGSTGAPFYFPRDLVVDTQSSIFHETFWRNTFAGGKEKSTLVIDSFGMGVWIGGIIT
                     YQAFRQIALSGAPISIITPGINKKEIFEAFKNIAGKFEQVILCGYPPFIKDLIDEGHL
                     HGIDWKKYNIKFLFAAEAFSEKFRDYLMKKVGMKDPLLNISNIYGSADIGTMAAESPL
                     SILIRRIASNKNKVFESIFPGVNKTPTLAQFNPLFVNFEAPEGVILLSGYNILPLIRY
                     SIGDHGGVSTFDDMSQTLKHSGINLKQEISAAKIGRAVSEMPFVYVYERIDLSTKLFG
                     AIIHPEHIKESLYHESLENYLTGKFTLSTVNDKHNNQWLEIHLEMKPNVSHSEELGNR
                     VKDIIIGSLLEKNAEYKNNYKMMPHKVTPKLFFWEYEDPKYFSRSGKQKWVQK"
     gene            complement(6104..6508)
                     /locus_tag="P5056_03035"
     CDS             complement(6104..6508)
                     /locus_tag="P5056_03035"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY62718.1"
                     /translation="MENEQELNKGKVLLVDDDPLIIRMYEYRLRHDGYKVFLAFDGEK
                     AIIEAKKEAPNIILLDLMMPKMNGVEALKFLKKEHDTKNIPVIILTNLGDDPSYINLT
                     KELGATEYLVKAETSLKQLTKKVDEIIYKHKK"
     gene            complement(6515..>7604)
                     /locus_tag="P5056_03040"
     CDS             complement(6515..>7604)
                     /locus_tag="P5056_03040"
                     /inference="COORDINATES: protein motif:HMM:NF026177.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cache domain-containing protein"
                     /protein_id="HRY62719.1"
                     /translation="KTLLPFISAFALLASSALVSFFYTSGHFDVFWILGHIIEIVGYV
                     ALAIWVWQYLKLRVREMLLLIFFSASLLVSIVVTLAFSMILIRQIELAMMNNLSANIK
                     LADYLIIHLKEESLSKARIFSSTVELRNALSKNDFGKIERLANNFAEQENISLLTIAD
                     KTGNVILRANQTTEKGDNMIVGKSGQLAAEGYSVATVDSFAGEKFAVKAISPVISPNG
                     AVMGYVIAGFILDNPFVDSLKKITGLDVSIYDKDKVAASTITGLDDRTRISGTNLSNS
                     FIETTVLKGGKAVTLNNDIVSKPFVSSYIPINDLDGGVVGMVSFAKSQREIFEIANTT
                     NILTLISVIIIMAILAAPIFKFSRRLLE"
BASE COUNT         2182 a         1780 c         1301 g         2341 t
ORIGIN      
        1 ttactacagg gcataaggtt aaaaacccca gaaggaaagt tcgtattacc aaagttgatt
       61 atttaacaac atttctcgga ttccgccttc ggctttctat gctggtatat gacttgtatc
      121 tagaaacttc ctacggggca ggcccccacc acaataagac taatgtgttt tgaatagatt
      181 aggataagtt tctcgccccg atagtaaagc ggagcttact acagggcata aggttaaaaa
      241 ccccagaagg aaagttcgta ttaccaaagt ggattattta ccaacagttc tcggattccg
      301 cctgcctgcc gtgcctgcgc acgcagggca ggcaggcctt cggctttcta tattgagata
      361 tgacttatgt ccagaaactt cctacggggt aaaaaacacc ccgtggttat ggggtgtttt
      421 aaggattctt tatttattaa gagaacgcaa ataataatat gccgaattcg cggcgacggc
      481 accggaagcg gcagctgtta tgttttgctt aaaactcccg aaggcgttgc acagatcccc
      541 cgccacataa gctcccgcga tatttgtttt catcgaaaag tcggtccgag cataaccatg
      601 gtcgtcaaga aaaacaccta tatcttttgc taatttgttt acaggcaaag accctatctc
      661 aacaaaaagc ccgtctattt ttagtttatt ggacccgtta aaatctttgt tcaaaacaac
      721 tccggaaaga ctatccgcac ccaaaaattc ttttatctct gttttgtata atatctctat
      781 cttgtctcgc attattttta ttttctcggt acttatcggc tcagcggtta gactgtcgcc
      841 cttcacaatc agataaacct tcttggcata ttccgaagcg agaagcgccg cgcttacgcc
      901 cgcatttcct ccgccgacga ctgcaatgtc cttccctaca tatatcggcc cgtcacaagt
      961 tacacaataa tgtattcccc tattctctaa ttccgactct ctcggcaaac ccatctttct
     1021 tctctctgag cccgtggcta aaataattgt tttcgccgta aaactttttt tatcttttgt
     1081 gcttaccaaa aacagctcgt ccttcttttc tatacttaga acttctccat cataaaattt
     1141 cgccccaaat tcctccgcct gctccttcat tgtttttgac aactcatacc catcggcatt
     1201 tttaacgccc gggtaattgt gtatcgtgcc ggccttggcg gcgtatcccc caaactcttt
     1261 tccgacaacc aatgtattca tggcaaatct gcccgcatac aaagcggcgg caaaccccgc
     1321 ggggccaaac ccgacgataa ttgtatcgta aatattgtcc atctagagtc ccaaaagctc
     1381 ctttatggta gctttatcaa aaccgattat cacttcatcc ccgatcttta taactggcac
     1441 gccggactga ccggtcatct tcaccatttc atttctggcc ttctcatcca aatatacatc
     1501 cttttctgta tattcaacct tattttctct aaaaaaatct tttgccattc tgcaatacat
     1561 gcaagaattc gtgctgtata ttaccactga tttcatatgc gttgttttat taatttataa
     1621 actattaaat gtcattttaa gccctcggga aagaaactgc aaatgtggtg ccgaccccct
     1681 cttccgaact gaacattatc gtgccgccca tcttctcgac caacatttta gtgataaata
     1741 gtcccaaacc ggtacccaaa ataccctgcg tatctttatt tgatgctcta aagaattttc
     1801 caaatatctt cccctgctga tccttcggaa tcccaaaccc ggtatccttt accttgatta
     1861 cgacattctt ttcgtctatg cagtcaatat ctatattaac tgttccgtta tttttattat
     1921 acttaatcgc atttcctatc aaattgacaa aaacttcttt ggtctttttg tcatccgcca
     1981 atatcttgtt gcaattatcg ctatcgttaa aaattatctt aatctctttc tctagcgcca
     2041 acgacccgag ctcctttaat atttctgata taaccggagc aataagcatc ggactagttt
     2101 ctatcttcat ggcaccggcg tcagagcgcg ctatttccag caaattattt accaattcat
     2161 tcaagtggtc gctggccatc ctaatggcgc cgagatcgtc ctgaacatct tttggaaaat
     2221 tcacctcaac atcccctacc aattcaagga aacctcttat ggcggttatt ggcgttctca
     2281 gttcgtgcgc ggccacgaac acaaattcat ctcgtagctt ggcttcggtc ctggccttct
     2341 ccatttctct ttcatgtgac tctttaagag caaccgcact ctcggccagt ttctcccgct
     2401 gatcaacttc ctttataacg ctccttatga ggaaaatacc tgtgacgacc atcaagaaca
     2461 ataaccccgc attcatcatc tggtcctcta ccgtcataga caagattgtt cttattagga
     2521 tgaacgtcca gagcgaaaac accaacaatt cagttgcgat aatctttacc gagaataagt
     2581 ggtgtttcaa aatcgcataa gaggtgagca ttataaacac ggacactgcg agcggggtca
     2641 gattaataaa gaaggtattt gaaaatacgg cgacaaatac aaggttgaaa aggaaaaatg
     2701 ccgaaaacat aatccctact ccggccaaaa gatatttaat ctgaatcctg gaaatcgggt
     2761 ccttttctga aattattttt ctggcgagag cccacactga cgccaaagta aaacaaaaag
     2821 taaaaacgga gaaccaggcg atgccgacgt taattgtttt cccaaaaaca gataagggcg
     2881 aaccgaagat cggaacggga acattcgttt tcgggacgct tatccccgta atcaaaaacg
     2941 gagtaagggt catgagggcg gtaattattg taacaatcgc cacgaatatt ccgcccttct
     3001 tagatatttt tagttcatca tgaggaaacg cccggataaa caaatataca aaagcattga
     3061 gaaatatagc ggtaaacatt accaacctaa cccacatcaa tttttctgaa taatcagagg
     3121 gctgcgtgct caaaagattt gatgcaagga aaaacgccaa aaaaatattc aggaaggcaa
     3181 aaataacatt cgtggcgctc tttctgttgt tgagaaaaac tacaagtccc agcaataaat
     3241 taaacaccac agacagagtg ctgatgatta tcgggaaagg tgtcgaaact aaatatgcct
     3301 cagatatatt catacggaaa tattatagac cgaatatttt tttaaactct cttatcatct
     3361 cttctctttt tgcaatttct gaaggtgaat tgtatcccgg gataagcatc tcatccatag
     3421 agatgagcga cctattactt ataatagtct gaccatttaa tatttttcta acacaataag
     3481 cgacagccgc tccgtttatc atggcggcac cgcccaactg cggccaagaa acaatggtct
     3541 tacccatttc caagagcgat tgcctcattc tttctgtaac attttccgga ccgacgtgtt
     3601 ttgtaattgt cttgcctata ccgaatttgt ccaatttggt aagcatttca taatttacgt
     3661 cccctattct tccgtggaaa aatttaggct gaggtttctc gtcatatctc tctacatcca
     3721 cgacggcgtt atctccgtta tccgcggcca taactaccgc gattttcctc ttcttggcct
     3781 gttcccttat caaatattta accgcaatat tatctatttc gtctaccaca atatctagac
     3841 cgttaaaaaa tttgtctata ttcttttttg taataccgtc cgggaaaatc tctacctttg
     3901 cataaggatt catctcatat atttgtcttg ccgccatccc caccttcaaa ctccccagat
     3961 cctgcgctcc cgcacggatt ctgttcatat ttgataaagc caaggaatcg tgatctgcaa
     4021 tctttatgtg ctttgctcca ccctgcatca caatacccag agcaacgcta ttcccgacac
     4081 tcaatccggc aattccgata gtggcattat aaaacctatc ttgctcgtcg gcgtttatca
     4141 aatttctatt cctggcggtt ctcaccaatt ggtatgactt gtcatccaag atgtgcgtta
     4201 aacatgaagc ccatggaaaa tatgcccact tgccatgttt agacaatgac gtcttttttt
     4261 taataccatc caaaaattcg tcaaagtgct tttgaaaatc agggtggtat accaaagaag
     4321 gattcgagac ggcaaagaat tccctcaact gctcaacata atcgtctgaa accctgtcta
     4381 tttttttatt ttttaaaagt ttcgacagct ccttactgtc cttctctttt tttaaatcaa
     4441 aaataatcgg ttccgaaacc accccctccc tgcttttccc ggataaaatt ttatctaatg
     4501 acataaaata tctaaattaa tatcaatgct tccattataa caaaaaaggt ccgacttcaa
     4561 aaaatcggac cccttatctg tgacatcaat ctatttttga acccattttt gtttaccgct
     4621 tctactgaaa tatttagggt cctcatactc ccagaaaaat aacttggggg ttaccttgtg
     4681 cggcatcatc ttgtaattgt ttttatattc ggcgttcttt tcaagcaaac tcccgattat
     4741 tatatcttta accctattcc ccagctcttc cgagtggcta acgtttggct tcatttccaa
     4801 gtgtatttcg agccactgat tattgtgctt atcatttacc gtggaaagag taaacttacc
     4861 tgttaaataa ttctccaaag attcatgata tagcgactcc ttgatatgct ccggatgtat
     4921 aatggcacca aaaagttttg tgctaaggtc gattctttca tatacataca caaatggcat
     4981 ttctgaaaca gcgcggccta tctttgcggc ggatatttct tgcttaagat ttatacctga
     5041 gtgtttcaga gtttgactca tatcgtcaaa agtggaaact ccaccatggt cgcctatcga
     5101 atatctgatt aacggcagaa tattataacc cgacaaaagg ataactcctt ccggcgcttc
     5161 aaaattaaca aagagggggt tgaattgcgc gagggtcggc gtcttgttta ctcccgggaa
     5221 aatagactca aaaaccttat ttttattaga ggcaatcctc cttataagta tgctgagagg
     5281 gctctcggcc gccatcgtcc cgatatccgc gcttccgtaa atgttcgaaa tattaagaag
     5341 aggatctttc atccctactt ttttcatcaa ataatctctg aatttttcgc taaatgcctc
     5401 ggcggcaaat aaaaatttaa tattatattt tttccaatct attccgtgca ggtgcccctc
     5461 gtctatcaag tccttaataa aaggagggta tccgcaaaga ataacttgtt caaacttacc
     5521 ggcgatattc ttaaaagctt caaaaatctc ttttttattt atacccggag ttattatgga
     5581 tatcggcgcc cccgaaagcg caatctgcct aaacgcctga tatgttatta tccccccgat
     5641 ccaaactccc ataccgaaag aatctataac aagtgtgctt ttttccttcc ctcccgcaaa
     5701 agtatttctc caaaaagtct cgtgaaaaat agaggattga gtatctacga ccaggtctct
     5761 cgggaaataa aaaggtgcgc cggtagagcc ggaggtggaa gtaaaaacgg ttgatttgtt
     5821 ttttagtgtt ccgtcaaaac aaagtttttc taatgggtat tttctgagat agtcggcttt
     5881 tgtcatcggt gggacaagct gaaaatcctt aaaagacctt atcttgccgg gatctatctt
     5941 gtttttcttt aaaaagtcct tatacgcggg gacccttacc gatgcggctc gaaaaagatc
     6001 taatgttttc ttgtcgccga tctttttcca cgccgaagaa ttattttctt tttgatattc
     6061 ttttatcttt ttaaaagaag catcgcgaga aaagatattc atactatttt ttatgtttat
     6121 aaataatttc atcaaccttt ttggtcaact gttttaggct cgtctccgcc ttaacaagat
     6181 actcagtagc acccagttct tttgtaagat ttatatagga aggatcatcg ccaagatttg
     6241 tcaaaatgat aaccggaata tttttggtat catgctcctt tttcaaaaac ttcaaagctt
     6301 caacgccatt catttttggc atcataagat ccaaaagaat tatgtttggc gcctcttttt
     6361 tagcctcaat tatagctttt tctccgtcaa aagccagaaa aactttatat ccgtcgtgtc
     6421 tcaagcgata ttcatacatt cttatgataa gggggtcgtc atccaccaac aaaaccttac
     6481 ccttatttaa ttcttgttcg ttttccattt tttgttattc caacaacctc cttgaaaact
     6541 taaatatagg agcggctaaa atagccatta ttataataac cgaaatcagc gtaaggatgt
     6601 tcgtcgtatt ggcgatttca aatatttctc tctgggattt ggcaaaagac accataccga
     6661 caactccccc gtctaaatcg tttataggaa tatagctaga cacaaacggc ttggaaacaa
     6721 tatcattatt cagcgttacc gcttttcctc ctttcaagac tgtagtttca ataaaactat
     6781 tactcaaatt tgttccggat attctggtcc tatcgtctag acccgttatt gtagaagcgg
     6841 cgactttgtc tttgtcataa atagaaacat ccaaccctgt tatctttttt agactgtcta
     6901 caaatggatt atctaatata aaaccggcga ttacatatcc cattacggcg ccgttcggag
     6961 aaataaccgg agaaattgcc ttaacggcga atttctctcc ggcgaaagaa tcgacagtag
     7021 caaccgaata tccctctgcg gccagctgtc ccgatttacc cacaatcata ttatccccct
     7081 tctctgttgt ctgatttgcc ctaagtataa cgttgcccgt cttgtcggcg attgttaaga
     7141 ggctaatatt ttcttgctcg gcaaaattat ttgccagcct ttcaatcttc ccaaaatcat
     7201 ttttgcttaa agcatttcta agttctaccg tggatgaaaa aattctggcc ttagacaacg
     7261 actcttcttt caaatggatg attaaatagt cggccaattt tatatttgcg gaaaggttgt
     7321 tcatcatggc cagctctatt tgcctgataa gtatcataga aaaagccaga gtaacaacaa
     7381 tagaaacaag gagcgaagcg gagaaaaata ttaaaagcaa catttctctt actctgagct
     7441 taagatactg ccagacccaa atcgccagag caacatatcc tacaatctca ataatatgac
     7501 cgaggatcca gaaaacatca aaatgacccg aggtatagaa aaatgatact aaggcagacg
     7561 aagccaatag cgcaaaagcg gatataaatg ggagaagagt cttt
//
LOCUS       DAOTEX010000040         7516 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3118
            NODE_6955_length_7516_cov_4.329312, whole genome shotgun sequence.
ACCESSION   DAOTEX010000040 DAOTEX010000000
VERSION     DAOTEX010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868142
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 7516)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7516)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 883
            CDSs (total)                      :: 833
            Genes (coding)                    :: 829
            CDSs (with protein)               :: 829
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7516
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6955_length_7516_cov_4.329312"
                     /isolate="AMR_MDS_3118"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>395
                     /locus_tag="P5056_03045"
     CDS             <1..>395
                     /locus_tag="P5056_03045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62720.1"
                     /translation="TEVFGPIHSNGGVRFDGLAHNIVSSALPDYNDPDHYGGDEFAVH
                     THVSPLDPEPPADVPSRTDVFEVGRQFPVPAVDFGKFTNDLSQIKTDAKASGKYFGSS
                     GKSGYHFVLKTDNTFDAYQVTALVPVPNN"
     assembly_gap    396..405
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <406..980
                     /locus_tag="P5056_03050"
     CDS             <406..980
                     /locus_tag="P5056_03050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62721.1"
                     /translation="GQFDWGTWSIKTETFLDNYPFPANGLIFAEDDVWVNGQINGARL
                     TIASGRFPENHGRETHITVNDNLLYTNYDGTDSIGLVAQGNINVGMISANTLRIDAAL
                     IAKNDRIGRYYYRPPSDNKERCAPYHTRDTITLYGMIGTNKRYGFAYTDGTGYQTRNI
                     IYDANLLFSPPPNLPLTNDQYQIVSWKEVK"
     gene            1238..1828
                     /locus_tag="P5056_03055"
     CDS             1238..1828
                     /locus_tag="P5056_03055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62722.1"
                     /translation="MKYGNLTLGQIEALVNKLGGMEEVRKILSGQGAPAPAPQAPSVF
                     LPALSVPTVPELIEPFVTKDHFNSVEGIWTGSDFNKEFRGMSVAPRKGYELTVRTLSM
                     PANDGEILKELGDPDGLKAEIDLYTLHQLIVMQKSGRRGLLTTDYANIFYLRNNEGQL
                     RAVSVSRDDGEWSVSVYSVSSPVRWSGGRRVFSRNS"
     gene            1967..2722
                     /gene="tpiA"
                     /locus_tag="P5056_03060"
     CDS             1967..2722
                     /gene="tpiA"
                     /locus_tag="P5056_03060"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HRY62723.1"
                     /translation="MKKIIIGNWKMNPSSVKEAVSLVSNIDKKISSNGNVVLCPPFIY
                     IGAVRGALSKKSSIKLGAQDCFYEEGGSFTGEIAPSMLKSMDVKYAIIGHSERRARGE
                     SNDIVNKKIRALLKKGIKVILCVGEEERDETGLYLKRIEEQVKEGLRGVSKNNFKNII
                     VAYEPVWAIGSDASKNCDPKDIKEISIFIKKILVDLSSREIGLSVQIIYGGSVDVKNA
                     PEIVSNGGVDGLLAGRASLDAKKFSDIINLLFS"
     gene            2728..3846
                     /gene="pgk"
                     /locus_tag="P5056_03065"
     CDS             2728..3846
                     /gene="pgk"
                     /locus_tag="P5056_03065"
                     /inference="COORDINATES: protein motif:HMM:NF012389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HRY62724.1"
                     /translation="MTLKSVDKITRLKGKRVLLRIDLNVPIENGMVEDDYRIKRSLKT
                     IEFLRKEGAKVIIISHRESLDDNSLSIFSKYFDIPFIDMHAALFSDKSLSFMKNGDAV
                     LLENLRMDPGEKKNSPIFAKKLASLADVFVNEAFSVSHRAHASIVGVPKLLPTYFGFL
                     FLDEVKNLSTAFSPEHPFVFILGGAKFETKLPLIRKFLGIADKVFVGGALANTFLREK
                     GFEIGKSKAEGSMFIPKDVLNNEKVVIPKDFVVKGEYCRCIKKADEIETGDVISDIGP
                     SFIDEVDKAVLSAKFVLWNGPLGMAESGFGEGTRSIAKTIAISDARSILGGGDTVAAI
                     KELGTQDNFTFVSTGGGAMLEFLASGSLPGIDIVAKQN"
     gene            3859..4590
                     /locus_tag="P5056_03070"
     CDS             3859..4590
                     /locus_tag="P5056_03070"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HRY62725.1"
                     /translation="MKKGKKIPIIFENEDVLVIDKPAGISVHQDGRKEEYTIAEWVLE
                     NYPEIKEVGEPLILEDGRKILRPGIVHRLDKDTSGVMVVAKNERAFNLLKGQFQDRKI
                     NKSYRAIAHGKFKDDNGIIDKPIGRSGSDFRAKNTGRYARGEMREALTYYKVLKRFDD
                     YTLLELRPKTGRTHQIRVHLKSISRPIVCDGIYGKSLRCPEWMGRLALHAYSLEFTLP
                     GGEKRTFISPMPKDFEAALDKIRGL"
     gene            4606..5013
                     /gene="rplS"
                     /locus_tag="P5056_03075"
     CDS             4606..5013
                     /gene="rplS"
                     /locus_tag="P5056_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005661552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HRY62726.1"
                     /translation="MKLGNIKISPVNLEERKKFDGKPGDTIRVSVRIKEGDKVRTQDF
                     EGLIIARKHGSEAGATITVRKVASGVGVERIFPLFSPSIEKIQIKKRGTVRRSKLYYI
                     HEKASKEIRKKMRSEVVKEEASINEKSDEKKSE"
     gene            5094..5777
                     /locus_tag="P5056_03080"
     CDS             5094..5777
                     /locus_tag="P5056_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62727.1"
                     /translation="MYDKTMGERGPLPKEKIKIRWSRNFAYAIGLISSDGSLSKNGRH
                     ISFVSKDKDQVLNFIKALHLKNIKIGKNYSGQYKSLSYRVQFGSVFFYNFLLSLGLTP
                     NKSKSIGKLNIPDKFFFDFLRGVFDGDGSTYSYWDKRWRSSFMFYTEFASASEDHIFW
                     LRNKIYEKLAISGHVTRDGKGSTLQLKYAKAESVVLLKKMFGPGRYLFLKRKRLKINK
                     MLCIVGEKV"
     gene            5779..5862
                     /locus_tag="P5056_03085"
     tRNA            5779..5862
                     /locus_tag="P5056_03085"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5813..5815,aa:Leu,seq:gag)
     gene            6224..6769
                     /locus_tag="P5056_03090"
     CDS             6224..6769
                     /locus_tag="P5056_03090"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HRY62728.1"
                     /translation="MLYTRKGDDGKTKLFDCDTRLLKSDEVFWALGNLDELNSFVGLL
                     KAKFNDSNIADVSYLEFLNNIQNDLFVIQAFVGGANKKLSIDRINVLEGKIKEIEKEI
                     PPITSFIVPGATEMSALSDVIRCVARRVERSAVDLFEKSPENVDENALIYLNRLSSFF
                     FAFARLLSHRAGVKEGAPKYN"
     gene            7326..7397
                     /locus_tag="P5056_03095"
     tRNA            7326..7397
                     /locus_tag="P5056_03095"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7359..7361,aa:His,seq:gtg)
BASE COUNT         2258 a         1390 c         1877 g         1981 t
ORIGIN      
        1 actgaagttt tcggccccat tcattctaac ggaggagtaa gatttgacgg tctcgcccac
       61 aatatcgttt ccagcgcctt accggattat aacgaccccg atcattacgg cggagatgaa
      121 tttgcggttc atacacacgt ttcgccctta gaccccgagc cgccggcgga tgttccctcc
      181 agaacagatg tttttgaggt cggtcggcag ttcccggtcc ctgctgtaga ttttggaaaa
      241 ttcacaaatg atctgtccca aataaaaaca gatgcaaaag cgtccggtaa atattttgga
      301 agttcgggca aatcaggata tcattttgtt ctgaaaacag acaacacttt cgatgcttat
      361 caggtgaccg ctctcgtgcc cgtgccgaat aattgnnnnn nnnnnaaggg cagtttgatt
      421 ggggcacatg gagcataaaa acggaaacat ttttggataa ttatcctttc ccggcgaatg
      481 gtttaatttt cgcagaggat gatgtttggg ttaacgggca aataaacggt gcgagattga
      541 cgattgccag cggccgcttc ccggaaaatc acggtcggga gacgcacata accgtgaatg
      601 ataatttgct ttatacaaat tatgacggca ccgatagtat aggccttgtt gcccaaggaa
      661 atataaatgt ggggatgatt agcgcaaata cgctcagaat agacgccgct ttaatagcaa
      721 aaaatgacag aatcggcaga tattattatc gtccgccgtc agacaataaa gagcgatgtg
      781 ccccttatca cacaagggat actattaccc tttatggaat gataggcaca aataaaagat
      841 acgggttcgc atatactgac ggaacaggct atcagacaag aaatataata tatgacgcaa
      901 acttgctttt ctctcctcct ccaaatttac cgttgaccaa tgatcaatat cagatagttt
      961 cctggaaaga ggttaaataa agaaattaca agccacttag tagacgggtt cctaagtggc
     1021 tttgttttgg gccgtataat ggccgtctga aaccttgaca aataagccta atgggtgtat
     1081 aatcataaaa gaattaaaaa gtaagtttta cgttctttta aataaagttc aagaaagaaa
     1141 ggaggtcggg acttaaacaa aaatgttgcg cgagcaaccc ctaaaacctc gggcagaggg
     1201 aagtccttcg acaagcagtc gaagagaagg agatgccatg aagtacggca atttgaccct
     1261 gggccagatc gaggcactcg tgaataagtt gggcggcatg gaagaggtcc gcaagatcct
     1321 ctcgggtcag ggcgcgcctg ctcccgcccc ccaggccccc tccgtcttcc tccctgcgct
     1381 gagtgtgccc accgtccccg aactcatcga gcccttcgtc acgaaggacc acttcaactc
     1441 ggtcgagggg atctggaccg gctccgactt caacaaggag ttccggggta tgtcggtcgc
     1501 gccgcggaag ggctacgaac tcacggtccg caccctctcg atgccggcga acgacggcga
     1561 gatcctcaag gagctcggcg accccgatgg tctgaaggcc gagatcgacc tctacaccct
     1621 ccaccaactc atcgtgatgc agaagagcgg caggcgcggt ctcctcacga cggactacgc
     1681 gaacattttc taccttcgca acaacgaagg tcagctccgc gcggttagcg tgagccggga
     1741 cgacggcgag tggagcgtga gcgtctactc cgtctcgagc ccggtcaggt ggagcggtgg
     1801 ccgccgggtc ttctctcgca attcctgatg accctctgaa acagtgtcac ttgagttttg
     1861 actctttgac actgtgacgc tctgaccctt tggcaacttg ccggagggtc ttttcttttg
     1921 ttttaaattt tctgtttact ttgataaatg ataaaattaa cacataatga aaaaaattat
     1981 tatcggaaat tggaaaatga atccgtcctc ggtaaaagag gcggtttctt tggtttcaaa
     2041 tattgataaa aaaatatctt ctaacggaaa tgttgttttg tgcccgccat tcatatatat
     2101 aggtgccgta aggggtgcac tttccaaaaa atcttctata aaattgggtg cgcaggattg
     2161 tttttacgag gaggggggct catttaccgg tgagattgca ccctctatgc ttaaatcgat
     2221 ggacgtaaaa tatgcgataa tcggacattc ggagagaagg gcaagggggg agtctaacga
     2281 tattgtaaac aagaaaataa gggcgcttct caaaaaagga attaaggtga ttttgtgcgt
     2341 cggcgaagaa gaaagagatg aaactggttt gtatttgaag cgcatagagg aacaagtaaa
     2401 ggaagggctt cggggcgtaa gtaaaaataa tttcaaaaat attattgtcg catatgagcc
     2461 ggtttgggcc atcggtagtg acgcttcaaa aaattgtgat cctaaagata taaaagaaat
     2521 ttctatcttt attaaaaaaa ttttagtaga tctttcttcc cgggaaatag ggttaagcgt
     2581 tcaaattatt tatggcggtt ctgtagatgt gaaaaatgcg cctgaaatag tttcgaacgg
     2641 cggagttgac gggcttttgg cgggtagggc gagcttggac gcaaaaaagt tttcagatat
     2701 tattaatttg ctattttctt aatctaaatg accctaaaaa gcgtagataa aattacccgg
     2761 ttaaagggga agagggtctt gcttaggatc gatcttaacg ttccgataga gaacggtatg
     2821 gttgaggacg actatagaat aaaaagatct ctcaaaacga tagagttttt gaggaaggag
     2881 ggtgcgaagg ttattataat aagccacaga gagtcgcttg atgataactc tctatccata
     2941 ttttccaaat attttgatat tccgttcata gacatgcacg cggctctttt ttcggacaaa
     3001 tccctgtcgt ttatgaaaaa tggagatgct gttttgttgg aaaatttgag aatggacccg
     3061 ggggagaaga aaaatagccc gatttttgcg aaaaagctgg cgtcgttggc tgatgttttt
     3121 gttaatgagg ccttttcagt atcacacagg gcgcatgcct ccatcgtcgg tgttcccaag
     3181 ttgcttccta cttatttcgg atttctattt ctggatgagg tcaaaaatct ctctaccgct
     3241 ttctcaccgg aacatccttt tgtgttcata ttaggcggag caaaatttga gaccaagttg
     3301 cctctaatta gaaaattttt aggaattgcc gacaaggttt ttgtcggggg cgcgcttgcc
     3361 aatacttttt taagggagaa gggttttgaa ataggaaagt caaaagcgga agggtcaatg
     3421 tttattccaa aagatgtatt aaataatgaa aaagtagtca tcccaaaaga ttttgttgtg
     3481 aaaggcgagt attgcagatg cattaagaag gccgatgaga ttgaaacggg agacgtgata
     3541 tctgatatcg gtccgagctt tattgatgag gtggacaagg ccgttctttc cgccaaattt
     3601 gttttatgga atggacccct cggcatggcg gagagcggtt ttggtgaagg gaccagaagc
     3661 atcgccaaaa caatagccat atccgatgca aggtctattt tggggggagg agataccgta
     3721 gcggctataa aagaactggg cacacaagac aatttcactt ttgtctcgac cggcggaggg
     3781 gcgatgctcg aatttcttgc gagcggctct ttgcccggga ttgacatagt ggccaaacaa
     3841 aactagagta ttccggtaat gaaaaaaggc aaaaaaatac cgattatatt tgaaaatgaa
     3901 gatgtcttgg ttatagataa accggccggt atttcggtgc accaggacgg cagaaaagag
     3961 gaatatacaa ttgccgagtg ggttcttgag aattatcctg aaataaaaga ggtgggggag
     4021 ccgctcattt tagaagatgg cagaaaaata ttaagacccg gcattgtcca tcgtttagat
     4081 aaggacacga gcggtgtaat ggtcgtcgca aagaacgaaa gagcttttaa tctgctcaag
     4141 gggcagttcc aggacagaaa aataaataaa tcatatcgcg ccatagctca cgggaaattc
     4201 aaagatgata acgggataat agacaagcct atcggaagat ccggttccga tttcagagcc
     4261 aaaaataccg gcagatacgc gagaggagag atgagggagg ctcttacata ttataaggtg
     4321 ctaaagaggt ttgatgatta cactcttttg gagctgaggc cgaagaccgg caggacacat
     4381 cagataagag ttcatctgaa gtcgataagt agaccaatag tttgcgatgg tatatatggt
     4441 aaaagtctaa ggtgccctga gtggatgggg aggctggcgc ttcatgcgta ttcccttgaa
     4501 tttactttgc caggaggaga aaaaaggacc tttatttcgc ctatgcctaa ggactttgag
     4561 gcggcacttg ataaaataag agggttgtga tagattctta ctactatgaa attgggaaac
     4621 attaagattt cacctgttaa tcttgaagaa agaaagaagt tcgacgggaa accgggcgat
     4681 actatcaggg tttctgttag aatcaaggaa ggcgacaagg taagaactca ggattttgag
     4741 ggcttaatta tcgccagaaa acacggttct gaagccgggg ctacaattac agtaagaaaa
     4801 gtcgccagcg gagtcggagt agaaagaatt ttcccattgt tttctccaag catcgagaag
     4861 atacagataa agaagagggg aacagtcaga agatcaaagc tctactacat tcacgaaaaa
     4921 gcttcaaaag agattagaaa gaaaatgaga agcgaggttg taaaggaaga agcttccata
     4981 aacgaaaagt cagacgagaa aaaatcagag taattttaaa aaagacggcc cgagccgtct
     5041 tttttcctat tttaattggt agaggctatc cccttgagaa aaatatccct cagatgtatg
     5101 ataagacgat gggggaaaga ggacctttac caaaagagaa aataaaaatt agatggtcta
     5161 gaaattttgc atacgcaatc gggcttattt ctagtgacgg gagcctgtcg aaaaatggta
     5221 ggcatatttc ttttgtttca aaagataaag accaggttct caattttatt aaagccctcc
     5281 accttaaaaa tataaagatt ggcaaaaatt attccggtca atataagagc ctttcttata
     5341 gagttcagtt tggtagtgta tttttttata atttcttatt gtcgcttggg cttacaccta
     5401 ataaatcgaa aagtatagga aaattaaata taccggataa attttttttt gattttttga
     5461 ggggtgtttt cgacggagat ggaagcacat attcatactg ggataaaaga tggaggtcta
     5521 gttttatgtt ctacaccgaa tttgcatctg ctagcgaaga tcatattttt tggctaagaa
     5581 ataaaatcta tgaaaaatta gccattagcg gccacgttac aagagatggg aaaggttcaa
     5641 ctctgcaact taagtatgct aaggcggagt cggttgtctt gttgaagaag atgttcggtc
     5701 ccggaaggta tttatttttg aagaggaaga ggttgaaaat aaacaagatg ttgtgtatag
     5761 taggggagaa agtttaacgc gccggtgttg aaattggtag acatttatcc ttgaggtgga
     5821 taagccagca atggcgtgga ggttcgagtc ctctccggcg cacataaaat ccccgttttt
     5881 ggggatttta ctttacgccg gaagcaaagc gcctgcgcgc tttgcgtgag gacttgaaaa
     5941 gcttctccca tatttatgag gagctctgct ccgaataaat gggaaagctg tactgaatct
     6001 gtaagattcg agtcctctcc ggcgcacaaa ataaacacac aaatgcgaag catttgtgtg
     6061 ttttgatatt ttgtagcgcc ggagaggact cgaaggccgg aagcctgcct gcgcaggcag
     6121 gcgggaccca ttctgcaaga atgggagctg aggcggggtc gcggccgagc gaagcgagag
     6181 ctgtgaccga gtcctcttct ccctcttctg ctataatctc tctatgctct acacacgaaa
     6241 aggggatgat ggaaaaacaa aattattcga ttgtgacact cgtcttctaa aaagcgacga
     6301 agttttttgg gcgctcggca atttagatga attaaactct tttgtcggtt tgcttaaggc
     6361 gaagtttaac gactcaaaca ttgccgacgt ttcatatttg gaatttctta ataatatcca
     6421 aaacgattta tttgttattc aggcttttgt cggcggcgca aacaagaaac ttagtataga
     6481 tagaataaat gttcttgagg gcaagataaa agagatcgaa aaagagatcc cgcctataac
     6541 gagctttatt gtcccggggg ctacagaaat gagcgctttg tctgacgtga taagatgcgt
     6601 agcaaggaga gtggagcggt cagctgtcga cctgtttgaa aaaagtcctg aaaacgttga
     6661 cgaaaacgct ttaatatact taaatagact atcgagtttc ttttttgctt ttgcgcggct
     6721 acttagccac agggcaggtg taaaagaggg ggctcctaag tataattaag gtattcaaga
     6781 cgttctgtgg attttccaca gaccatattg tttaccacgc atcttttcgg gtatataata
     6841 caagagtgcc taatgtaaaa tgcaaaagat gcgataaaag tttctatgca aaaccgtcgt
     6901 ggttaagcag aggaaatgga aaatattgtt cgaataagtg tagttttgaa tcaagaaaga
     6961 gcggcaagac ggttctttgc gatatctgtg gtaagggggt ttacaaaacc gcccgggaat
     7021 taaagaggtc taaaagtaaa aaattcttct gtagcaagtc gtgtcagaca tgctggagaa
     7081 actctttctt tgttggagat aagcatgcca actggaagga aggggaatct tcttatcgag
     7141 gagtattaaa gaggaataat gtccctgaaa tatgcttgtt gtgtaagaca aaagatgcta
     7201 gggttcttga ggctcatcac ctggatcgaa atcgaaagaa tagtagttta aataaccttg
     7261 tttggttgtg tcgaaattgt cattttttga tacatcatta taaggaagag gagaagaaaa
     7321 ttatggtgtc tgtagtgtaa cggttcgcac tcgactctgt gaaagtcgca gaatgggttc
     7381 aaatcccatc agacacccaa aaataagtag tcccgactga aaagtcggga cgcttatatt
     7441 tttggagtgt atgacggggt ttgaaggccg gaagcgggcc cctttctgca agaaagggag
     7501 ctggggcggg gtcgag
//
LOCUS       DAOTEX010000041         7418 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3118
            NODE_7080_length_7418_cov_3.799674, whole genome shotgun sequence.
ACCESSION   DAOTEX010000041 DAOTEX010000000
VERSION     DAOTEX010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868142
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 7418)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7418)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 883
            CDSs (total)                      :: 833
            Genes (coding)                    :: 829
            CDSs (with protein)               :: 829
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7418
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7080_length_7418_cov_3.799674"
                     /isolate="AMR_MDS_3118"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1906
                     /locus_tag="P5056_03100"
     CDS             <1..1906
                     /locus_tag="P5056_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62729.1"
                     /translation="LGDSSISETAGGYLYNYTGGDQTVSFGFSTCACDVSASTWDCPK
                     TSFSSATNYATGCQERVGTTYKAYWKGAPVSGGVKVSAVTTFDKTIAIKGGTPGDKKI
                     LASDATGNASWKTAAELGLGGGASVSGTAGVVPVFNVDGNGLADSYINQTASGEYIYS
                     KGSTVPIAFSGSCPCDKDSNVADCPVSFVSPTIIGTSASSYSCTDRIASASWTVYGPS
                     SAGGSLNFFDKTTADKFAALGVVPNTTIRGLTNVIGGFKYVDNNQGANKILVSDVNGL
                     AHWKNISDLAVCTDTTSAVTPTSQTFTTPGSNTFVVPQGVTELTATLWGAGGGGGTDN
                     TGSAVDGSGGGGGGFIGNIKFKVKTGDAFNITVGAGGAGGGRASNGGLPGAAGGDSII
                     TGSANGTSISIMANGGKGGGSWDGGIGEDRGGAGGGWGYNPSSINLNTAIVTESGQKG
                     EGAGANYGGKGGDAGGKSYGGGAGGAGYSGCNGCSGGQRPGSAPGGAGAGGTWDGIGL
                     GAAGAPGKVVISYAAGGTTSTKCCIPGINCESSSNDVPNPAWKTLSGNVSYTKTGGIV
                     FIRINGANSTGNYGTLPAGYRPGVAIRQNVPTNTVYYDYVTVGTDGAVNVTVAASGNM
                     YQVFSYPAGQ"
     gene            2015..2446
                     /locus_tag="P5056_03105"
     CDS             2015..2446
                     /locus_tag="P5056_03105"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF012304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HRY62730.1"
                     /translation="MPHQKIIAYTDGGARGNPGIAGSGAYITYADGHVIKEAHRSLGV
                     KTNNFAEYEAVILALEEIKKFFGKEKLVELDIEVRMDSELVQRQLTGQYRVKDKGLQQ
                     QFIKLNNLKSEFPHVNFVHVRREDNKDADRLSNVAMNLQEK"
     gene            2479..3027
                     /locus_tag="P5056_03110"
     CDS             2479..3027
                     /locus_tag="P5056_03110"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HRY62731.1"
                     /translation="MTDNELISKYLEGDETALKSLIERYLKPIYSFVYRLTGNGQDAE
                     DISQEVFVKVWKNLNKFDESKSFKTWLFTIAKNTAYDSLRKKKEIVFSDLEDEDGEGG
                     VEEEISDSEILPEMALIKSEDVAGLEAAIKKLPFNYRAVIFLKETEELTFDEIGRILN
                     KPLNTVKSHYRRAILRLRKILI"
     gene            3174..3572
                     /locus_tag="P5056_03115"
     CDS             3174..3572
                     /locus_tag="P5056_03115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62732.1"
                     /translation="MSQKHIIFRHIDPPKDLLEEIFNKIDKNSRQRAGAKLFASMLGA
                     TSSLGIFVFVFRYAAEVFLESGAYSYLSLLFSDPSFVLSYSNGLALSIIESAPVLEIS
                     ILFFAVYAFLGSFKYAVENMGNLSLKNRLA"
     gene            3581..4084
                     /locus_tag="P5056_03120"
     CDS             3581..4084
                     /locus_tag="P5056_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62733.1"
                     /translation="MKTYITKMKQFVETDNFKRSIKVVGILAVITLIFQAGVFMGFRK
                     ASFYGEFGNNFRNNFEGDRGEFRGMMGRDIPNAHGVVGNVIDVALPSVVIAGDDGVEK
                     IIFVDEKTIIRKFREDISADKLIVGDNVVVVGNPGRDGIIDSRLIRIMPAPLTPVAAS
                     STNLDIK"
     gene            4086..5840
                     /locus_tag="P5056_03125"
     CDS             4086..5840
                     /locus_tag="P5056_03125"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HRY62734.1"
                     /translation="MSNIFPKVKKFAAQNKITSAIIFTVILISGYYTYNYFFGTTTET
                     RYIMGAVEKGTIISSISGSGQVSAYNQVDIKPKASGDIVWVSMKVGQSVYAGQALASI
                     DSTDAKKAVADAELDLAETKLNFDKAVAQAPIDYEKKLETLQKYKDDLDKTYEDSFNN
                     ISNAFLELPTVMTGIDNILYGYDFNPITKDWNVTAFKNLYDGSDRDLIVSLADVAEKD
                     YKTARAAYDKNFTNFKNLTRYSARTEIEKSLAETLETTKAIAQAAKSENNLLDTVIDI
                     AETKDKTLNSNINTFKTNLKGYLSTINTRLSSLLDQNSSIVSSKQSILDMEREISVLK
                     VNNPTGVNPIDLQISQNTINKKEVALQDLKNDLADYTIRAPFAGIIAKVNSKIGDTIS
                     SGTAVASLITKQKIAEISLNEVDVAKVELGQKATLTFDAVEDFSVTGEVAEIEAVGTV
                     SQGVVSYNIKITFDTEDDRIKPGMSTSASIVTEVKQDVLYAPNGAIKNKAGKNYVEMF
                     TDKKFVVNATSTQGITSDVDPVEQSVEVGISNDTNTEIISGLKEGDLVITRTTTGGTK
                     TTTTSSMGLPGLGGGPGR"
     gene            5881..6588
                     /locus_tag="P5056_03130"
     CDS             5881..6588
                     /locus_tag="P5056_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005834475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY62735.1"
                     /translation="MIRIENITKTYNSGDISFQALRGVSFEIEDGEFVAIMGPSGSGK
                     STLMYILGALDTPTLGKYFLDNKDVSKLNDDELAEIRKKKIGFVFQSFNLLPRTTVLR
                     NVALPLIYAGVPAEKRREIAGKALVSAGLDEAHFYHLSNQLSGGQIQRVAIARALVND
                     PTLILADEPTGNLDTKTGEIVLGTFQKLNEEEGRTIVLITHEPDVAEHAHRIIHIRDG
                     LIVSDSKEHKRRIINRK"
     gene            6619..>7418
                     /locus_tag="P5056_03135"
     CDS             6619..>7418
                     /locus_tag="P5056_03135"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRY62736.1"
                     /translation="MKTFDLLSETFSALFSNKVRSGLTVLGIVIGIGSVITMVSIGQG
                     AQNSIEESINSMGSNLLSIMSGTQRGPGVSVSAGRGSAKSLKLSDAEAIKEQISQIKA
                     VASEVSSRYQVTAKGTNTNTTVTGTVPDYTIVKNVEVDLGSFLAEQNIKSASKVAVLG
                     PSARDDLFGEDADPVGKTVRIKNIDFKVIGVTKEKGGSGPNNQDDVIYIPITVAQRYF
                     SGDEYVSTINVAAESSDAMTAVEEDITNLLITRHKIKDPTVLDFSIMN"
BASE COUNT         2219 a         1357 c         1856 g         1986 t
ORIGIN      
        1 gttgggggat tcatctattt cggagacggc cggcgggtat ctctataatt ataccggtgg
       61 agaccagaca gtatcgttcg gtttttcaac atgtgcttgt gatgttagcg catcaacatg
      121 ggattgtcct aaaacctctt tttctagcgc aacaaattat gctacaggat gtcaggagag
      181 agtggggacc acttacaagg cttattggaa aggcgcacct gtatccggcg gagtaaaggt
      241 gtcggcagta actacattcg acaaaacaat tgctattaaa ggaggcaccc cgggtgacaa
      301 aaagattctc gcttctgacg ctacaggaaa tgcttcgtgg aagactgcgg ctgagcttgg
      361 tcttggagga ggtgcctctg tgtccggaac ggcgggtgtt gtgcctgtgt ttaatgtaga
      421 cggtaacggg ttggctgatt cttatatcaa tcagacagct tccggtgaat atatttattc
      481 taagggaagc acagttccga ttgcttttag cggcagttgc ccttgcgaca aagacagcaa
      541 tgtagcggat tgtcccgtca gcttcgtgtc gccaacaata ataggcactt ccgcaagttc
      601 atacagctgt actgatagga tagcgagtgc ttcgtggacg gtatatggac catcaagcgc
      661 cgggggtagc ttaaatttct ttgataaaac gacagctgac aaatttgcgg ctttgggagt
      721 agtgccaaac accacaattc gcggtcttac gaatgtgatt gggggattta agtatgtaga
      781 taataatcaa ggcgcaaaca aaattctcgt ttctgacgtc aatggtcttg ctcactggaa
      841 aaatatttcc gacttggctg tttgtacaga tacaacttcg gccgttaccc caacttcgca
      901 gacatttaca accccgggat ctaatacttt tgtcgttcca caaggagtta cagagttaac
      961 cgctacactt tggggcgcgg gtggtggagg aggaacagac aatacagggt ctgctgtcga
     1021 tggatcgggt ggaggcggtg gtggatttat cggtaacata aaatttaaag ttaaaactgg
     1081 agatgccttt aacataaccg tgggtgccgg cggggcgggt ggcggtcgtg cttcaaacgg
     1141 tggtttaccc ggcgcggcag gaggcgattc tataataacc ggcagcgcca atggtacttc
     1201 tatttctatt atggcaaatg gcggtaaggg tggtgggtct tgggatggtg gtataggaga
     1261 ggatagagga ggagctggag gtggatgggg gtataaccct tccagcatta atttaaacac
     1321 agctattgtt acagaaagtg gtcaaaaagg tgagggtgcc ggtgccaatt acggcgggaa
     1381 aggaggagac gccggtggta aatcttatgg aggaggggcc ggaggcgccg gatatagcgg
     1441 ctgtaatggc tgtagcggag gacaaagacc gggaagcgct cccggaggag ctggtgcagg
     1501 aggaacttgg gacggtattg gtttaggagc tgctggggct cccggtaagg tcgtaatttc
     1561 ctatgctgcg ggtggcacta cttcgacgaa gtgttgtatt cccggtataa attgtgaatc
     1621 atcttctaac gatgttccaa atccggcgtg gaaaactctt tccggaaatg tatcatacac
     1681 aaagaccggt ggaattgtgt ttataagaat aaatggagca aatagcaccg gaaattatgg
     1741 aacacttcct gccggataca ggccgggtgt ggcaattagg cagaatgtgc cgaccaacac
     1801 tgtctactat gattatgtca ctgttggaac agacggagct gtaaatgtta cagtggcggc
     1861 tagcggtaat atgtatcaag tcttctcata tcctgccggg caataattat aaaagtctta
     1921 aaaccacccc taacggggtg gtttttaatt gccccacact ttgtgagatt cgctttgcct
     1981 tggcggggtt ttgttctggt gctaatattt tcctatgcca catcaaaaaa taatcgcata
     2041 tacagacggg ggagcgaggg ggaatcccgg gattgccggt tccggagcat atatcactta
     2101 tgccgacggc cacgtcataa aagaggcgca tagatcgctc ggggtaaaaa caaataattt
     2161 cgccgaatat gaggcggtca tattggcgct tgaagaaata aagaaatttt ttggaaaaga
     2221 aaaattggtt gagcttgata tagaagtgag aatggatagc gaactcgtgc agagacaact
     2281 taccggtcaa tacagagtga aggacaaggg gctccaacag cagttcataa aacttaataa
     2341 tctcaagtcg gaatttcctc atgtaaattt tgttcatgtg agaagggagg acaataaaga
     2401 cgccgacaga ctgtcgaacg tcgctatgaa tctgcaagaa aaataaccat taacgccttt
     2461 ttctggtaga ctattctcgt gacagataac gagcttatat ctaaatattt agagggagat
     2521 gaaaccgcgc ttaaatcatt aatcgagcgt tatttgaagc ctatatatag ttttgtttac
     2581 cgtttaacgg gcaacggcca agatgccgaa gatatttcgc aggaagtgtt tgtgaaggtt
     2641 tggaaaaact taaataaatt tgacgagtca aaaagtttta agacgtggct ttttactatc
     2701 gcaaaaaata cggcctatga ttccttgagg aagaaaaaag aaattgtttt ttccgatctt
     2761 gaggatgaag acggcgaggg tggggttgaa gaggagatat ctgattcgga gattttgccg
     2821 gaaatggcgc ttattaaaag cgaggacgtg gccggattag aggcggcaat aaagaaactc
     2881 ccgtttaatt atagggcggt tatattttta aaagagacag aggagctgac cttcgacgag
     2941 attgggcgaa ttttgaacaa gccgttaaat acagtcaaaa gccactacag aagggctatt
     3001 ctaaggctta ggaagatatt aatttaaaat tatcatcaag ctctcaaact tgtcattcct
     3061 gcgcaggcag gcggaaatct agaatactac ttgaaagtat ggattcccgc ctgcacggga
     3121 atgacagaac aacgaactgc accaaaattg tttaatggtt cgtattaaat aatatgagcc
     3181 aaaaacacat tattttcaga catatagacc cgccaaaaga tcttttggag gaaattttta
     3241 ataagataga taaaaatagc agacagcgcg cgggcgcaaa actttttgca tctatgctcg
     3301 gtgcaacttc gtctctgggt atttttgttt tcgtctttag atatgccgcg gaagtgtttt
     3361 tggaatccgg agcttattct tatttgtcgc ttttattttc agacccgtct tttgtcctgt
     3421 cgtattcaaa cggactagcg ctttctatta ttgaatccgc cccggttctt gaaatatcta
     3481 ttttattttt tgccgtttac gctttcttgg gttccttcaa gtatgcggtt gaaaatatgg
     3541 gtaatttatc attaaaaaat cgtctagctt aattaaaaat atgaaaacat acataacaaa
     3601 aatgaagcaa ttcgtggaaa ccgataattt taagagatca attaaggtgg tcggcatcct
     3661 ggccgtaatc actttaatat tccaagccgg agtatttatg ggcttcagga aggcttcttt
     3721 ttatggtgaa tttggcaaca atttcagaaa taattttgaa ggagacagag gtgaatttag
     3781 gggaatgatg gggagagata ttcccaatgc ccatggggtc gtcggtaatg tgatagatgt
     3841 ggcgttgccg tcggttgtca tcgccggtga tgacggagtt gagaaaataa tttttgtcga
     3901 cgaaaagacc attattagaa aattcagaga agatataagc gcggataaac ttattgtcgg
     3961 tgataatgtt gttgtcgtcg gtaatccggg ccgagacgga ataattgatt cgagacttat
     4021 tagaataatg cctgccccct taacacccgt ggccgcctct agcactaatc ttgatataaa
     4081 ataatatgtc taatattttc ccaaaagtaa aaaagtttgc ggcgcaaaac aaaataacta
     4141 gcgcgataat ttttacggtt attcttatct ccggatatta tacttataat tattttttcg
     4201 gaacgaccac ggaaacgaga tatataatgg gcgcggtgga aaaggggaca ataatttcct
     4261 caatttccgg tagcggtcag gtctctgctt ataatcaggt agatatcaaa cccaaggcgt
     4321 cgggtgatat tgtctgggtg agtatgaaag ttggtcaatc tgtgtatgcc ggacaggcgc
     4381 tcgcttctat agacagcacc gatgctaaga aggccgtggc tgacgcggaa ctcgatctcg
     4441 ccgaaacaaa gctaaatttt gataaggctg tagcacaggc tcctatcgat tacgaaaaaa
     4501 aattagagac gctacaaaaa tataaagatg atttggacaa aacttatgaa gattcgttca
     4561 ataatatatc caatgcattt ttggaattgc ctacggtaat gaccggaatc gataatattt
     4621 tatacggata cgatttcaat cctattacaa aagattggaa tgttactgct tttaaaaatc
     4681 tttacgacgg ctccgacagg gatcttatcg tgtcgcttgc ggatgtcgcc gaaaaagact
     4741 acaagacagc tagggccgct tatgacaaaa attttactaa tttcaaaaat ttgacgagat
     4801 attccgccag gactgaaatt gagaagtcgc ttgcagaaac cttagaaaca acaaaagcta
     4861 tcgctcaggc cgcaaaaagt gagaataacc ttttggacac ggttatagat atcgcagaga
     4921 caaaggacaa aacattaaat tcaaatatca atacctttaa aactaatctt aagggttatt
     4981 tgagcacgat aaacacaaga ctgagttcac ttttggatca aaacagttca attgtgagct
     5041 caaagcaatc aatcttggat atggagagag aaatttccgt attaaaggtt aataatccta
     5101 cgggggttaa tccgatagat ctacaaatat ctcagaacac tataaataag aaagaagtcg
     5161 ctttgcagga tcttaaaaac gacctggctg attatacaat tagggcaccc tttgcgggta
     5221 ttatagccaa ggtaaattcc aagataggag atacaatctc ttccggcacc gctgttgcga
     5281 gtcttatcac aaaacaaaag attgcggaga tttctttgaa tgaggtagat gttgcaaagg
     5341 tggaattggg tcaaaaagcg actctgacgt tcgatgctgt agaagatttt agtgtaacag
     5401 gagaagtcgc tgagattgag gcggttggga cagtttccca gggcgttgtg agttacaata
     5461 ttaaaataac ttttgatacg gaggacgata gaataaagcc ggggatgagc acaagtgctt
     5521 ccatcgttac tgaggttaag caggacgttc tttatgctcc taacggcgcg ataaagaata
     5581 aggccggtaa aaattatgta gaaatgttta ccgacaaaaa atttgttgtt aacgcaacca
     5641 gcactcaagg cataacttcg gacgtggatc cggtagaaca atccgtagag gttggtattt
     5701 caaacgacac taatactgag ataataagcg gcctaaaaga gggggatttg gttatcacaa
     5761 gaacaaccac aggcgggaca aaaacaacta caactagctc aatgggactt cccggtttag
     5821 gaggaggtcc gggcagatag tcgtttgaaa agaattatca aattaaaaat ttactgaagg
     5881 atgataagga ttgaaaatat aacaaaaaca tataattccg gcgacataag ttttcaggcg
     5941 cttcgcggag tttcgttcga aatagaggac ggagaatttg ttgcgataat gggtccgtcc
     6001 ggatccggta aatcgacact gatgtatatt ttaggagcgc tcgatactcc gaccttgggt
     6061 aaatattttt tggacaataa agatgtttct aagttgaacg atgacgagct ggcggagata
     6121 aggaaaaaga agattgggtt tgtttttcaa tcgtttaatc ttttgccaag gacaacagtt
     6181 ctaagaaacg tggctcttcc ccttatttat gccggagtgc ctgcggaaaa gcggcgagag
     6241 attgccggta aggctcttgt ttccgccggt ttggatgagg cgcattttta ccacctgtca
     6301 aatcaacttt caggagggca gatacagcgt gtcgcgattg cgagggcgct tgtaaatgac
     6361 cctacgctta ttctcgccga cgaacctacc ggaaatctgg atacaaaaac aggggagatc
     6421 gtgctaggca ctttccagaa attaaatgag gaagaaggca ggacaatcgt tcttatcaca
     6481 cacgaaccgg atgtggccga gcatgcacac aggataattc atataagaga cggcttaatt
     6541 gtttctgatt caaaagaaca caagaggagg attatcaatc gtaaataatt tctggtaact
     6601 gtatagttaa attttaaaat gaaaaccttt gatcttttga gtgagacgtt ttcggcgctt
     6661 ttttccaaca aagtgcgttc gggactgact gttctcggta tcgtcatcgg tatcggttct
     6721 gttattacaa tggtttccat aggtcaaggt gcccaaaatt caatcgagga gagtataaat
     6781 tcgatggggt cgaatttgtt gtcgattatg tccggaaccc agagggggcc gggggttagt
     6841 gtgagcgccg gtaggggttc ggctaagtct cttaagctca gcgacgccga ggctatcaag
     6901 gagcagattt ctcagattaa ggcggttgcc tcagaggtat ccagcagata tcaagttacc
     6961 gctaagggaa ctaataccaa tacgaccgtc acgggaacgg ttccggatta tactattgtt
     7021 aagaatgtcg aggttgacct cggttctttt ctcgcggagc agaatataaa gagcgcttca
     7081 aaagtagcgg tattggggcc ttccgccaga gacgatcttt ttggggagga tgctgacccg
     7141 gtaggaaaga ctgttcgtat aaaaaatatc gattttaaag ttattggtgt tacgaaagag
     7201 aagggcgggt cgggtccaaa caatcaagac gatgttatct atatcccgat aactgtcgcg
     7261 cagagatatt tttccggcga tgaatatgtg agcactataa atgttgccgc tgaaagttct
     7321 gatgcaatga cggcagtcga agaagatatt acaaacttgc tcataacaag acacaaaata
     7381 aaagatccga ctgttctcga cttcagcatc atgaatca
//
LOCUS       DAOTEX010000042         7400 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3118
            NODE_7111_length_7400_cov_4.085364, whole genome shotgun sequence.
ACCESSION   DAOTEX010000042 DAOTEX010000000
VERSION     DAOTEX010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868142
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 7400)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7400)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 883
            CDSs (total)                      :: 833
            Genes (coding)                    :: 829
            CDSs (with protein)               :: 829
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7400
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7111_length_7400_cov_4.085364"
                     /isolate="AMR_MDS_3118"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..635)
                     /locus_tag="P5056_03140"
     CDS             complement(<1..635)
                     /locus_tag="P5056_03140"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HRY62737.1"
                     /translation="MDKEMQCGNCPMCKMGMGGMHKYAGCVKKLFALAVFLMFLSFAY
                     SAFIYAGAYADSIQPGSYRSFSVSGEGKVTAVPDIASFTFSVITEGGKDLGALQKENT
                     EKMNKAIEFIKGKDVESKDIKTSNYSLNPRYQYFDCARPLTSSVQPCPPPEIVGYTIN
                     QTVSVKIRDFSKIGEIMSGVVAVGANNVSQLSFGIDDQTMVEAGARSEAIM"
     gene            complement(745..1860)
                     /locus_tag="P5056_03145"
     CDS             complement(745..1860)
                     /locus_tag="P5056_03145"
                     /inference="COORDINATES: protein motif:HMM:NF039195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PsbP-related protein"
                     /protein_id="HRY62738.1"
                     /translation="MKNKGFVNILLVTALIVALTAAGFFIFDRSLQKEIEPNNELVVP
                     DDVSTSADKLSNTISNLLTYTNTKYGYSLSYPNDWIIEPEQRIVDQYNEIVSLVNIHN
                     PDKSLGVNISISRKEPGIKYVASSQSKLSINGSTETAYLFPKGYECGMADPAAKDCSF
                     FIVAIQHNGFWYDLRAGGHAEAVAEPYTSIFSSFKFTGTGIKADEPDVVSSDSWRRYS
                     NKRYNYSFKYPAGAIIETAPANKIPEACLSIKYKDAFIYISNPKDSTEAPCGPTGVGL
                     GYTPDQRDIEIGGILYVAKGFEYSLQQLKVDLAKSGQSLSSNDKDETNQWFDISSVNG
                     NMRVVYGVNSGTGVKLTDYELTDRSELISSVLGSLTF"
     gene            complement(2130..5567)
                     /locus_tag="P5056_03150"
     CDS             complement(2130..5567)
                     /locus_tag="P5056_03150"
                     /inference="COORDINATES: protein motif:HMM:NF013625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HRY62739.1"
                     /translation="MSNSLKKYLSICLVVFSFVFIGGVQVASAADNNMLAQLASLKAI
                     LQELLTKVQGGQLAAVSAYGSFPVTGPAAGTAYTHGNNITVTWDPSGVSGYVYLKLYR
                     QTDGREMSSSWVPNSGSYTFSAGPTMNGMSFVGQFKAGIFNNASGAFTEASGQYFTVS
                     APSAAPALKVSSPNGGETVNVGNYFFVNWNKDALPNASTVTLQIVNSAGGAIFSNSSV
                     PNSGSYWMRLAVSGQFKAKVSSGSVSDESDGFFTSVDTSSAAAPTCTINTDKDTYNLG
                     ESVVISYTSQGATYATYKSNYPKEDLVTPGDKLETARKDMYPAKVVGNPVIKLAVYNQ
                     AGVEGTCSKTISVVLPLKVSSPNGGEIYQKYTNFSVVWNKEAIEPLPNGNTVNLKFYN
                     SISSSEQSVPNTGTFTSQLPAGQWKVKVSSLDGSVSDESDGFFTVAESAPVAAGSYTV
                     SQNNSLIYSLRAVRAGAEVALAGFKFTNTGNEPVNIRRIAMQASPSGAYKILNGQQIS
                     IWDGSIRVGTLNFVNIDKAVATLDTPINIPSGTHEIIIKGTLIESDVSGRRLAINYDG
                     TNNGLSGNYGSGVNSGVTILPNSATTNTQGVIIYKNIPTFEIVSNGGLFMNNVDTYKV
                     KVTNPAATDIALSQLTFSVMATGGTPSGFTLYADGIPVSAPVNPSNNTVKIAFDTNSP
                     TKIIPAGSSRTYTLRCASLSDVANISETLSIALKADFSFSGAGTAVGLKPANNIVWSP
                     RTSTTLSANDSSYENVSDWFNGFGLPGFPAGGGQDFPMQTWTRGGSEEPTPVAACARA
                     GEYVGESPASLPKKCCPGLVPDQLNPSLALGYKCIKSTPNITDVSAKAANKFEMAAGD
                     SAYISGTNLGGNVEVYIDGIKSEVRQASDTLIIAIVPLSLRDGFSYGVWVQNEKGKSD
                     VVQVKVVRGGISCKAEGEFWNPALSPIGTACCAGLTAETQPLLPGTSGRPYVKCVKSP
                     QALSCKSSSGKSMTLDEARSIAKTGDCAKLKLTGIDDCRTNNNGIWWLGAEMDSCFRA
                     CIINVDSKSSEAWAGCFGGLSADSNENVVSDFSVPCTQLTYDLRFGARDSGTGGQVTK
                     LQEYLSSAGYLDVEPTGYFGALTTQAVKIFQGKNGIPSMTGGVGQLTRNKIKEVSCK"
     gene            complement(5698..>7400)
                     /locus_tag="P5056_03155"
     CDS             complement(5698..>7400)
                     /locus_tag="P5056_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62740.1"
                     /translation="NSTLYANRVSLISNAVSWSTNFKTDVARKLNLKEAVKISLLTGD
                     GSSVVKTWTQANTGISSIPFAIEMKEGKYKIKVESIQRPGVTGESYVFNITHNYFSAV
                     QAKAGIVRGVGKISLTYPKGGEIIKHGSVFKTTATSLVPYEKFTTLKLIKSDGTEVYS
                     SIRPGVGSFIFTSMPKIINGYELNGQYKIQIIPFSNPALAVESKLFTITDATTVLPPT
                     TSVPTTPATSVVTPVVNINLVYPTNNSTITYGTSFYVKWNMANFGNKMVSLSVTDSTG
                     YITYVTSPKMNNGSLYFSANQFNGVPAVGKYKIRITAAENAYTAKDKFSVNRTLYRSN
                     LFGESLVNFVAPAPVAPVTTSPVSAPTRAALPTTPSIVVTVPPRVTPGYLSDGQLTVN
                     WTAANVTGSVRIELLNSAGVLVTTLSISRPITPATFIVDIPAAIATGQYKVKITGAGA
                     SGISAPFTITKPVFGTTYTPSTGVAKITVTSPTGGTFSRTKIIQVKWTQAGLTGNVKV
                     ELLMAGKAPVYIGTMAAVNGYINYTIPAKTLVGNYVIKVSSLTNTAVSATSPAFRIWY
                     "
BASE COUNT         2018 a         1728 c         1514 g         2140 t
ORIGIN      
        1 ttcattatgg cctctgatct ggctcccgcc tctaccattg tctgatcatc aataccgaag
       61 ctgagctgag aaacattatt ggcgccgaca gcaacaactc ccgacattat ttctccgatc
      121 tttgagaaat ccctgatttt tacagaaaca gtctgattga ttgtataacc tactatttca
      181 ggaggagggc aaggttggac actgctcgtt aatggtcttg cacaatcaaa atactggtat
      241 ctagggttca agctgtaatt gcttgttttt atgtctttag actcgacatc ttttcctttt
      301 ataaactcta tcgccttatt catcttctcg gtattctctt tttgaagagc tccgaggtct
      361 ttaccgcctt ctgttattac gctaaaagta aatgaggcga tatccggcac agctgtcacc
      421 ttcccctccc ccgaaacgct aaagcttctg tatgaacccg gctgtataga atcagcataa
      481 gcaccggcgt aaataaacgc gctgtatgca aaagaaagga acattaaaaa taccgctaac
      541 gcaaataact ttttcacaca gcctgcatac ttatgcatac cgcccatccc cattttgcac
      601 attgggcagt tgccgcactg catttcttta tccatatcca tttttattta attgataatt
      661 attttaataa tattataaat taattatagg ctaaaataat cccaaatcaa aaccgcccca
      721 tgccggggcg gttttatatt tattctaaaa cgtcagagat cctaaaacag aactaattag
      781 ttcacttcta tccgtaagct cataatcagt caatttaact ccggtgcccg aattaacgcc
      841 gtatacgacg cgcatattcc cgtttacaga gctaatgtca aaccactgat tagtttcatc
      901 tttatcatta gacgaaagac tctgaccgct tttagctagg tctaccttca attgttgcaa
      961 actatactca aagcctttgg ccacatatag aatcccgcca atttctatgt ctctctgatc
     1021 aggagtataa cccaatccga cgccggttgg tccacaaggc gcctcggtcg agtcttttgg
     1081 attgctaata tatataaaag catccttgta ttttatagac aaacaagctt ctgggatctt
     1141 gttcgcagga gccgtttcta ttattgcgcc tgcaggatat ttgaatgaat aattatacct
     1201 tttattggaa taccgtctcc aactatccga gctaactaca tctggttcat ccgcttttat
     1261 ccctgtcccg gtaaacttaa aagaagagaa aatgctcgta tatggctctg caacggcctc
     1321 tgcgtggcca cccgctcgaa gatcatacca gaaaccgtta tgttggattg caacaataaa
     1381 gaaagaacaa tctttggcgg ccggatctgc cataccgcat tcataacctt ttggaaacaa
     1441 ataagcggtt tcagtagaac catttattga aagtttggat tgcgacgagg ctacatattt
     1501 tatccctggc tctttccgac ttatcgagat atttacgccg agggacttat ctggattgtg
     1561 aatattaaca agtgatacta tctcattata ttgatccaca attctttgtt ctggttcaat
     1621 aatccagtca tttggatatg ataaagaata tccatatttt gtatttgtgt atgttaataa
     1681 atttgaaatt gtattactca atttatctgc tgaggtgcta acatcgtcag ggacgacaag
     1741 ctcattattt ggctcaattt ccttctgtaa acttctgtca aaaataaaaa aaccggcggc
     1801 cgttagcgca acaattaatg ctgtaaccag cagaatattc acaaaaccct tatttttcat
     1861 aaaaaaaata attacttatt attttataat tctatatcaa aataggcacg aatgacctcc
     1921 tccccgggtt ccgctccgct acccccgggg tttccttttt cggcatgagg agccctcatg
     1981 aacacacatg agtgtgtgga gtggactaca gtcttacatt cagtccccgc cctccaaagt
     2041 acataaacgg ggtagttgtg ggaaagctcc gggcggggtt ttggcaatta aatccaaacc
     2101 gccccatgcc ggggcggttt tttcaataat tatttacaag aaacttcttt gatcttattt
     2161 cttgttaatt gcccaactcc gccggtcata cttggaattc cgttcttgcc ttggaatatc
     2221 tttaccgcct gagttgttag agcaccgaag tagccagtcg gttcaacgtc caaatatcct
     2281 gcggagctta gatactcttg taatttagta acttgcccgc ctgtgccact atccctcgct
     2341 ccgaaacgca aatcgtaggt cagttgtgtg cagggtactg aaaagtcaga aactacgttt
     2401 tcgtttgaat ctgctgaaag acctccaaaa catcccgccc aagcttcgga ggatttgcta
     2461 tccacattaa taatacaggc cctaaaacaa ctatccattt ctgcacctag ccaccagata
     2521 ccattattgt ttgtcctaca gtcatctatg cccgtaagtt ttaatttggc acaatcccct
     2581 gttttagcga tgcttctggc ctcatctagt gtcatagact taccgctaga agatttacag
     2641 gataatgctt gcggcgactt cacacactta acataaggcc ttccagacgt gccaggtaac
     2701 aaaggttgtg tctcggcggt aagccccgcg cagcaagcag taccgatagg gcttagagct
     2761 gggttccaaa actcaccttc tgccttacaa gagattcctc ccctaaccac ctttacctgc
     2821 acaacatcac ttttaccttt ctcgttctga acccaaacgc cataagagaa accatctctc
     2881 aaagatagcg gcacgattgc aattattaga gtatctgatg cctgtcgaac ctctgatttt
     2941 atgccgtcaa tataaacctc gacgttgcca cctaaattgg ttcctgaaat atatgccgaa
     3001 tctcctgcgg ccatctcaaa tttattagca gctttcgcag atacgtccgt aatatttggt
     3061 gttgatttta tacatttata gccgagggca agggacggat taagttggtc tggaactaaa
     3121 cctggacaac attttttagg tagtgaagct ggagattcgc ccacatactc accagcccta
     3181 gcacaagcag ccaccggcgt cggctcttcg ctacctcccc ttgtccatgt ctgcattggg
     3241 aaatcttgac cgcctcctgc cgggaatcct ggtaaaccaa aaccattaaa ccaatcgcta
     3301 acattttcat aagaagaatc attggcagag agggttgtgc tcgtccttgg tgaccatact
     3361 atgttgttcg caggtttcaa accaacagcg gttcccgcgc cgctgaaaga aaagtcagcc
     3421 ttaagcgcga ttgatagtgt ctcggaaata ttggccacat ctgataaaga ggcacaccta
     3481 agtgtatatg tcctcgaaga tccggctgga ataatcttcg tcggagaatt ggtgtcgaag
     3541 gctatcttta ctgtattgtt tgaaggattt acaggcgcag atacaggaat gccatccgcg
     3601 tacaaagtga agccagacgg agtacctcct gttgccataa ctgagaatgt taactggctg
     3661 agcgcgatat ctgtagcggc agggtttgtg accttgacct tataagtatc gacgttattc
     3721 ataaacaaac ctccgttgct tacgatttca aaagtaggga tgtttttgta aataatcaca
     3781 ccttgtgtgt tagttgttgc ggaattaggt aaaattgtta cccccgaatt aaccccgcta
     3841 ccatagttac cactcaaacc attgtttgtg ccatcataat ttatagcaag gcggcgacca
     3901 gatacgtcgc tttctatgag cgtgcccttt attataatct cgtgggtacc agatgggata
     3961 ttgatagggg tatctaaggt agctacagcc ttatctatgt tcacaaagtt gagagttcca
     4021 actcttattg aaccgtccca aatactgatt tgctgtccat ttaatatttt atatgcccca
     4081 gatggagaag cctgcatggc aattcgtcta atattgaccg gctcattccc tgtgtttgta
     4141 aatttgaacc ctgccaatgc gacttctgcc cccgccctga cagccctaag actatatatc
     4201 agcgaattat tctgagaaac cgtgtatgag ccagccgcca ctggcgcgga ttcggctacc
     4261 gtaaagaacc catcgctctc atccgaaaca gagccgtcta aagaagaaac cttgactttc
     4321 cactgacccg cgggaagctg agacgtaaaa gtaccggtat ttggcacaga ctgctccgaa
     4381 gaagaaatgg aattataaaa ctttagatta acagtattgc cattgggaag tggttctatg
     4441 gcttccttgt tccaaacaac cgagaaattg gtatatttct gataaatctc tcccccgttt
     4501 ggagaggaga ctttgagggg caacacaaca ctaattgttt tactacacgt accctcaact
     4561 ccagcctgat tataaaccgc cagctttatt acgggattac caacaacctt tgcgggatac
     4621 atatcctttc tagcagtttc taatttgtct cctggagtaa caagatcctc ttttggataa
     4681 ttagatttgt atgtcgcgta agtggctccc tggcttgtgt aggagatcac aacactttcg
     4741 cccaaattat atgtatcctt atccgtgttg atcgtacaag tcggcgccgc agcagaagaa
     4801 gtatcaacgg aagtaaagaa cccgtcgctc tcatccgaaa cagaaccact ggaaaccttg
     4861 gccttgaatt gacccgatac ggccaatctc atccaatatg aaccgctgtt tggcacagaa
     4921 ctatttgaga agatagcgcc gccggctgag ttgacgattt gcaaggtaac cgtagacgca
     4981 ttcggaaggg cgtctttgtt ccagttaacg aaaaaatagt tgccgacgtt tactgtctct
     5041 cccccgtttg gagaggagac tttgagggca ggagcggctg aaggagcgct aaccgtaaaa
     5101 tattgaccgc tagcctcggt aaaggccccc gatgcattat tgaagatgcc cgccttgaac
     5161 tgaccaacaa aagacattcc gttcattgtg ggccccgcag aaaaggtata ggaacctgag
     5221 tttggcaccc agctagagct catttcccgt ccatcggtct gtctataaag cttaagatac
     5281 acataaccgg agacccctga cggatcccat gtgacggtta tattgttgcc atgagtatat
     5341 gcggtgccgg cggcaggacc tgttacgggg aaagaaccat aagcagaaac agcggcaagt
     5401 tgccctcctt gcacttttgt taaaagctct tgaagtatcg ccttcaaaga tgccagctgc
     5461 gcaagcatat tattatcggc agccgaagcg acctgcacgc cccctatgaa cacaaaagaa
     5521 aaaacaacca aacaaatact taaatatttt tttaatgaat tactcatatt attttttaat
     5581 actcaataaa aactttttaa taaaaattac aactcatata ttatagcacg cccctctcct
     5641 ccttgtgata cacaatatga aaaaccgccc ctttatgggg cggttttaaa gtgattatta
     5701 ataccagatt ctaaaggctg ggcttgttgc ggaaaccgcg gtatttgtca gcgaagacac
     5761 cttgattaca taattaccca caagagtttt tgcgggaata gtgtaattta tatacccatt
     5821 tacagcagcc attgtgccta tatagacggg ggccttaccc gccattagaa gctctacctt
     5881 tacatttccg gtcaaacctg cttgcgtcca ttttacctgg ataatcttgg ttctgctaaa
     5941 cgttcccccc gtcggggaag ttaccgtgat tttggccacc ccggtagacg gcgtgtatgt
     6001 agtcccgaag acaggcttcg tgatagtaaa tggggcgcta attccagacg ctcctgcacc
     6061 tgtaatcttg accttatact gaccggttgc gattgccgcc ggaatatcaa ctataaatgt
     6121 cgcaggtgta ataggcctac ttatacttag tgttgtgact aaaacgccgg cggaatttaa
     6181 taattcgatt cttacgctcc ccgtaacgtt agcggcggtc cagttaaccg taagttgccc
     6241 gtcggaaagg tatcccggtg ttacgcgagg cggaacagta accacaattg atggggttgt
     6301 cggtagagcg gctcgtgttg gtgcggatac gggcgaagtc gtgacaggag ccactggcgc
     6361 aggagcaaca aaattgacca agctctcccc aaataagttc gaacgataaa gggtcctatt
     6421 aacgctgaac ttgtcttttg ccgtatatgc attttcggcg gcagttattc gtattttgta
     6481 ttttcctact gcaggcactc cattaaactg gtttgcgctg aaatagagag aaccgttatt
     6541 catcttagga gatgtgacat acgttatata ccccgtgcta tctgtgaccg acaacgaaac
     6601 cattttgttg ccaaaattcg ccatattcca cttaacatag aatgaagtgc cgtaggttat
     6661 ggttgaatta tttgtcggat aaacaagatt aatatttaca accggggtca cgacagaggt
     6721 ggccggcgtt gtcggtactg aagttgtcgg cggaagcacc gtagtggcat ccgtgattgt
     6781 aaataacttg ctttcaacag ctaaggcagg gttgctgaaa ggaattattt gaattttgta
     6841 ttgtccattc aattcatagc cgttaattat cttaggcata gacgtaaata tgaacgagcc
     6901 gactcccggt ctaattgagg aataaacctc cgtgccgtct gatttaatca atttcaatgt
     6961 ggtaaatttc tcgtatggaa ccaaagaggt ggcggttgtt ttgaagacac ttccgtgttt
     7021 aataatttcc ccgccttttg gatatgtgag agatatttta ccgacgcccc tgacaattcc
     7081 ggccttagcc tgaactgccg agaagtaatt gtgagttata ttaaatacat aactttcgcc
     7141 cgtaactccc ggtctctgga ttgattctac ttttattttg tatttgccct ctttcatttc
     7201 tatcgcgaat gggatgcttg agatacccgt atttgcctgg gtccatgtct ttactacaga
     7261 tgagccgtca ccggttaaaa ggcttatttt aaccgcctcc ttaagattta actttctagc
     7321 aacatcagtt ttgaaattag tgctccatga tactgcgttt gaaattaagc tgacccgatt
     7381 tgcataaagt gtggagttgt
//
LOCUS       DAOTEX010000043         7333 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3118
            NODE_7204_length_7333_cov_3.924842, whole genome shotgun sequence.
ACCESSION   DAOTEX010000043 DAOTEX010000000
VERSION     DAOTEX010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868142
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 7333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 883
            CDSs (total)                      :: 833
            Genes (coding)                    :: 829
            CDSs (with protein)               :: 829
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7333
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7204_length_7333_cov_3.924842"
                     /isolate="AMR_MDS_3118"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="P5056_03160"
     rRNA            complement(<1..579)
                     /locus_tag="P5056_03160"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            complement(1139..1828)
                     /locus_tag="P5056_03165"
     CDS             complement(1139..1828)
                     /locus_tag="P5056_03165"
                     /inference="COORDINATES: protein motif:HMM:NF017373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62741.1"
                     /translation="MDTGIIQTSGAYLGTAFQNVSNQIVMLLPKVLLAVVILLAGWVV
                     AVAIAEVVSRIVKSLKVDSALKGAGVGDLVEKAGYNLNSGAFLGGLIKVFIIVVFFLT
                     SLEIVGLSQVTVLLGNLVMQILPNLLVVVVLIFAAAIIAEAAKKFVVASARASGVPSA
                     GFLGQMTKAAIWLFAILLSLIQFQVGATTLNTIFLGVVVAISLAFGLAFGLGGKDVAA
                     RLLEKWVSKFE"
     gene            complement(2184..2717)
                     /gene="rplE"
                     /locus_tag="P5056_03170"
     CDS             complement(2184..2717)
                     /gene="rplE"
                     /locus_tag="P5056_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HRY62742.1"
                     /translation="MDSIKQNYIKNIGAIKEKLGIKNIMAVPKITKVVVSVGTGSFRD
                     KKKNAIVVDKIAKITGQKSVSKGAKKSVASFKIRQGDLVGHMVTLRGDRMYGFLNKLI
                     NVAIPRMRDFRGLDSKSVDDMGNFTMAVKENTVFPETSDEDLKDVFGMGITVVTTAKN
                     KKDATEFLKIIGFPFKK"
     gene            complement(2722..3027)
                     /gene="rplX"
                     /locus_tag="P5056_03175"
     CDS             complement(2722..3027)
                     /gene="rplX"
                     /locus_tag="P5056_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006310544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HRY62743.1"
                     /translation="MIVKKNDNVIISSGKDKGKKGKILKSIPAKGAVIVEGVNLLKKR
                     QRAKKAGQKGQTVSVAMPIKASAVMLFCSSCAKGVRAGFNKIADKKVRVCKKCNKEI"
     gene            complement(3037..3408)
                     /gene="rplN"
                     /locus_tag="P5056_03180"
     CDS             complement(3037..3408)
                     /gene="rplN"
                     /locus_tag="P5056_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013985279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HRY62744.1"
                     /translation="MIQPRSIVKIADNTGAKIGRIFKVLGGSKKKYARIGELVVLSVQ
                     TAEPRKPVKKKDVLTAVVVRQTAPMRRKDGSYIRFDENAVVIVDKVKKEPIGARVFGP
                     IPREIAELGYQKIASLAPEII"
     gene            complement(3453..3698)
                     /gene="rpsQ"
                     /locus_tag="P5056_03185"
     CDS             complement(3453..3698)
                     /gene="rpsQ"
                     /locus_tag="P5056_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009206576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HRY62745.1"
                     /translation="MEKKAKRLKGVVVSDKMDKTVVVKVDRYVEHPKYEKRFTISKKF
                     KAHDEKEEYNEGDLVIIEECRPISKDKTFKVVEKVQK"
     gene            complement(3700..3885)
                     /gene="rpmC"
                     /locus_tag="P5056_03190"
     CDS             complement(3700..3885)
                     /gene="rpmC"
                     /locus_tag="P5056_03190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HRY62746.1"
                     /translation="MKKVDFKNKTTEELNKLLTEKRNALKDFNFKSSGGAAKNVKTGS
                     VTKKEIARILTALNSKK"
     gene            complement(4029..4445)
                     /gene="rplP"
                     /locus_tag="P5056_03195"
     CDS             complement(4029..4445)
                     /gene="rplP"
                     /locus_tag="P5056_03195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010938605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HRY62747.1"
                     /translation="MLTPKKVKHRKWQRDRRNFSSPLPESRGTTLAFGSYGLKAVDTA
                     RVTSNQIESARKVMSRYVKKFGKVWIRIFPDRPITKKPAEVRMGKGKGDPVGYCFEVR
                     PGRVIFEIDGVDAATAKESLRGAADKLPVKTVIVTR"
     gene            complement(4452..5132)
                     /gene="rpsC"
                     /locus_tag="P5056_03200"
     CDS             complement(4452..5132)
                     /gene="rpsC"
                     /locus_tag="P5056_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HRY62748.1"
                     /translation="MSHTVHPYSHRLVVLRDWKSRWFGTRGKYQEYLKADIILREFLQ
                     KKLRGAQVDSVEMERGENYYKIIIKTAKPGIVIGRNGEAAAKLREEVIRKLTKNNIPL
                     PKDVKIEVEEVRAPEAKSAIVAEMIAEGLEKRLPFMRVIRQALEKITANRDVLGAKIT
                     ISGRLGGAEMSRVETVKKGRLPLQTLRADIDFAREKAHLPYGDIGIKVWIFKGEVFAD
                     KRRNTTHK"
     gene            complement(5264..5596)
                     /gene="rplV"
                     /locus_tag="P5056_03205"
                     /pseudo
     CDS             complement(5264..5596)
                     /gene="rplV"
                     /locus_tag="P5056_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005007675.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
     gene            complement(5605..5946)
                     /gene="rpsS"
                     /locus_tag="P5056_03210"
     CDS             complement(5605..5946)
                     /gene="rpsS"
                     /locus_tag="P5056_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012469416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HRY62749.1"
                     /translation="MARSLKKGPFTEPKLLKKIEGKRPDSTPMIKTWSRKSQISPEMI
                     GFLFGVHNGKNFIEVRPTEEMVGHRLGEFAPTRKFTKHGGKMQKELEMKKKEAEITAA
                     KAAKVEAPAKK"
     gene            6244..6759
                     /gene="infC"
                     /locus_tag="P5056_03215"
     CDS             6244..6759
                     /gene="infC"
                     /locus_tag="P5056_03215"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HRY62750.1"
                     /translation="MDKRVRINNQIRVPEVRVIDDDGTNLGVMRVEEAIRMAESKNLD
                     LVEISPNAVPPIAKITDYGKFQYLENKKQKAAKAKTQVTEVKNIQVSIGISDRDLELK
                     AKNASEFLKEGNKVRIVLTLRGREKYLNKDFLKERVNRLFKLLTEEYKIIEDLKPAQR
                     GQAMTIERLKK"
     assembly_gap    6792..6801
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            6875..7063
                     /locus_tag="P5056_03220"
     CDS             6875..7063
                     /locus_tag="P5056_03220"
                     /inference="COORDINATES: protein motif:HMM:NF013771.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HRY62751.1"
                     /translation="MKTNNSYLKRLKVTKNGKVISRKAGKCHFNAKESRSKQLGASKM
                     VSFTMKKADISRYLPHNQ"
     gene            7093..>7333
                     /gene="rplT"
                     /locus_tag="P5056_03225"
     CDS             7093..>7333
                     /gene="rplT"
                     /locus_tag="P5056_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HRY62752.1"
                     /translation="MPRVKKAVHALKRRRKVLKQAKGFRFGRGAKQKQAQEAILHAGK
                     YAFAHRRKKKRNMRKLFQEKIGAASKENGTSYSKLM"
BASE COUNT         2082 a         1671 c         1290 g         2280 t
ORIGIN      
        1 ttccctcgcg gtactagttc actatcgatc tctgagagta tttagcctta gcggttagtt
       61 ccgccagatt cccccgagct actcatgtct cgaggtactc aagaataatg attaaaaagt
      121 ttctttattt tcaactacag gattgttacc ttctatgatt ttgctttcca gcaaattcgt
      181 ctaacaaaga aatttataac ttctgttaac accattacct tacaacccct atgtcttcct
      241 ttaacagaaa actaggtttg ggctattccc ttttcgctcg ccactactaa gagaattcca
      301 gaacgtagaa tatagaatgt aaccgaagct acatcccata attccacgct ctttatttgg
      361 tttcctttcc tttgggtact gaaatgtttt acttccccaa gttcacttct tacaacaagt
      421 gtaagatctt ccgatatgac tcggaagggg tttccccatt cggacatctc cggatcaaag
      481 gttgctaggc acctccccga agcttatcgc agccatgcta cgtccttcgt cgtctctaca
      541 gagtcaaggc atccaccata cgctcttaaa tttcctatta ggaaatttaa aaaccacgtt
      601 atatacgatt acccacattc cactgatcta ctagaagagt ggaagtttgc ccattacata
      661 agaaaagacc tcaaaatgta atggactaaa ttttcaaata acgttctttc tgtcaaaacc
      721 gattctctgt cctccagaat cttctaaatc ttagaaaact ttggagggcc tagaatttta
      781 aagaaaaaga ccgcgtcttg tgcggtcaaa gaaagcgcag gaaaaaatca ggggctactt
      841 tcttatccgc aactttaaat taataatcat gcttaaggta tgtaaaatct atacgaaacg
      901 aatctaaatg tcaacatcgt ttctgtgtgt ataacaaacg acaactactt catagcatat
      961 ttgccagata cttgtcaacc ccccaccact tttggcgatg gactgaaata gaaacggaag
     1021 ccctcgatac ttcaaaaaaa ccttgttatc caacaaaaat aagccaaaag tggtgggggg
     1081 taaattaact aaaaacccac cgccagggtg ggtttttaaa acttctataa aaaagaaatt
     1141 actcaaactt gcttacccac ttctccaaga gtctagcggc aacgtccttg ccacccaaac
     1201 caaaggcgag tccgaaagcc aaagaaatgg cgacaacaac tccgaggaag attgtgttaa
     1261 gagttgtagc acctacctgg aactggataa gtgagagcaa aatggcaaaa agccaaatgg
     1321 cggcctttgt catctgaccc aagaaccctg cggaaggaac acctgaagct cttgctgaag
     1381 ccacaacgaa tttctttgcg gcttctgcaa taatggcggc ggcaaaaata aggacaacaa
     1441 caaccaaaag atttgggagt atctgcatta ccaagttccc aagaagaact gtaacctggc
     1501 taagaccgac gatctcaaga gatgtaagga agaagacaac aatgataaat acttttatca
     1561 aacctcccaa aaaggcacct gaattcaaat tgtatccggc cttctcaacg agatccccta
     1621 cccccgcacc cttcaaagct gaatcaactt tgagagactt aacaattctt gaaacaactt
     1681 ctgcgattgc tactgctact acccaacccg ccaaaagaat taccactgcg agcaaaactt
     1741 ttggtaacaa cataactatc tggttgctaa cattctggaa agctgttcca agataagcgc
     1801 cagacgtctg aataatacct gtatccattt tatttttttt aataattaat aattttataa
     1861 tttaaaaaaa caaacccgat tttcgaccct gaaaactagg gcttgctgtg ctaatcacct
     1921 atataaatat aacattttgg aagaccgctt tccaacgatg ctgtggataa ccctgtagga
     1981 aagtccgtat tcccagagtg gattatttac caacatctct cggattccgc ctgcctgcgc
     2041 aggcaggcct tcggctttct atgctggtat atgacttatg cctagaaact tcctacaggg
     2101 caggccccga tagtaaagca tagcttacta cattagcggg ttttaaaaac accccgctta
     2161 agcggggtgt tttttaaaat ttattatttt ttgaaaggaa atcctattat tttcaaaaat
     2221 tcagtagcgt cctttttgtt cttagctgta gtaactactg ttatgcccat tccgaataca
     2281 tccttcaaat cttcgtcgga tgtctcaggg aaaactgtgt tctccttaac ggccatcgtg
     2341 aagttaccca tatcatctac ggacttagaa tccaatcctc tgaagtctct cattcgggga
     2401 atagcgacgt tgatgagctt gttcaaaaat ccatacattc tgtcccctct caaagtaacc
     2461 atgtgtccga cgaggtctcc ctgtctgatc ttaaatgaag ctacggactt cttggccccc
     2521 tttgatactg atttctgtcc tgttattttt gcaatcttgt ccacaacgat agcgttcttt
     2581 ttcttgtccc taaaagaacc tgttccaaca ctgacaacca ccttagttat ctttggaacg
     2641 gccataatat ttttaatacc gagcttttcc ttgattgccc cgatattctt tatgtaattt
     2701 tgttttatag aatccatctt tttatatctc tttgttgcac ttcttgcaga ctctgacctt
     2761 tttgtcggca atcttattaa aaccggccct tactcctttg gcacagcttg aacagaaaag
     2821 catgactgct gatgccttta taggcatagc aacggaaacc gtctgacctt tctgacccgc
     2881 cttctttgct cgctgtctct ttttcaaaag attgactcct tcaacaataa cagcaccctt
     2941 agcaggtata gactttaata tctttccttt ctttcctttg tcctttccgg aagaaattat
     3001 tacgttgtcg ttctttttaa ctatcattgt attgtattaa attatttcag gtgccaaaga
     3061 agcgatcttc tggtatccca attctgcgat ctctctagga ataggaccga agactctagc
     3121 gccaataggc tctttcttaa ccttatcaac tataacgaca gcattttcat caaatctgat
     3181 gtagcttccg tcctttctcc tcatcggagc tgtctgtctt actacaacag cggtcaaaac
     3241 gtctttcttc ttaacaggct ttcttggctc cgctgtctgg accgacaaaa ccacgagctc
     3301 gcctattctc gcatacttct ttttggaacc accgagcacc ttgaagatcc ttccgatctt
     3361 tgctccggtg ttatcagcta tctttactat ggatcgtggt tgaatcataa atcaattatt
     3421 aattagaaat tacgaattac gaatttaaaa aattatttct gtaccttttc tacaacctta
     3481 aatgttttgt ctttggagat cggtctgcac tcttcgatta tgactaaatc tccctcgtta
     3541 tactcctctt tttcgtcatg cgccttgaat ttttttgaga ttgtaaatct tttttcgtat
     3601 tttgggtgct ctacgtatct gtctaccttt acaacaacgg ttttatccat cttgtcggag
     3661 accacaacac ccttcaatct ctttgctttc ttttccattt tatttctttg agtttagggc
     3721 tgttaatatt ctcgcgattt ccttctttgt tacactgccg gtctttacgt ttttagcagc
     3781 acctccggag ctcttgaaat taaaatcctt tagcgcgttt ctcttctcgg ttaaaagctt
     3841 gtttagctct tcggtcgttt tgtttttaaa atctactttt ttcatttacc ctgttaagta
     3901 aaaatttgct tttactttta taattaactt tttaacaatt atctttcaat aactaaactt
     3961 ttctaatttc tttcacaata tttagcaaat tttaaatttt tataaaaatt ttactaaaca
     4021 gggtgaaatt atcgggttac gatgactgtc ttaaccggta atttgtccgc agctcctctc
     4081 aagctttcct tggctgtagc ggcgtctact ccatctattt caaaaattac tcttcccggt
     4141 cttacctcga aacagtatcc tacaggatct cccttaccct tacccattcg gacttcggca
     4201 ggcttttttg ttattggtct atcagggaat attcttatcc aaaccttacc aaatttcttt
     4261 acatatctgc tcataacttt tctggcagat tctatctggt ttgaagttac tctggcggtg
     4321 tcaacagcct ttagaccgta tgatccaaaa gcgagggttg ttcctcttga ttccgggaga
     4381 ggagaactga agtttctcct atctctctgc cacttcctgt gttttacttt ctttggggta
     4441 agcattgtat tttatttatg agtggtattt cttctcttat cggcaaaaac ctcccctttg
     4501 aatatccaga cctttattcc gatatcccca taaggaagat gcgccttttc tctagcaaaa
     4561 tcgatatctg ctctgagcgt ctgcaaagga agtctgccct tctttactgt ttcaactcgg
     4621 ctcatttctg ctcctcccaa tctacctgaa attgttatct ttgctccaag aacatctcta
     4681 ttggctgtta tcttttcaag agcctgtctg attactctca taaatggaag tctcttctca
     4741 agaccctcgg caatcatttc ggccacgata gcagacttag cttccggagc tctgacctcc
     4801 tcaacttcaa tctttacatc cttaggaagc ggtatgttgt tttttgtaag ttttctgatg
     4861 acttcttctc tcaatttggc cgcggcctct ccgtttcttc cgataacgat acccggcttt
     4921 gcggtcttta taatgatctt gtagtaattc tcacccctct ccatctctac agaatcaacc
     4981 tgtgcgcccc ttagtttttt ctgaaggaat tctctgagta tgatatcagc tttcaaatac
     5041 tcctgatatt ttcctcttgt accaaaccag cgagatttcc aatctcttaa aactacgagt
     5101 ctgtgtgaat atggatggac ggtgtgtgac ataataatta cgaattaaaa attaagaatt
     5161 acaaattata aaattatttt tttgttgttt ttttaaagac tttcttcttg gctacaggct
     5221 ttgtggccgc cttcttaaca gcaggcttct cggcctttac cggctctttt gattcgagct
     5281 ccagcaaaac atggctggtc ctcttcttaa taaggaatga tctaccgaaa gctcttggca
     5341 ttcccctctt catcacaaat ccgccgtcta ctcttacgct ctttacaaaa agactatctg
     5401 caggaagttt gaaattatgt gttgcgtttg caatagcaga ttttatgagc ttaaccatag
     5461 gttcggccgc tctttttccg gagaacgaaa caagagcaat agcctcatcg gcttttttgc
     5521 ctcttattaa atcagcaagg agtcgcacct ttctaggagc ctgtctgtat ttatttaatt
     5581 cagccttgat tatcatgtta ttatttattt cttagctggg gcctcaactt tagcggcctt
     5641 agcggcggtt atctccgcct ccttcttctt catctcaagt tccttctgca tctttcctcc
     5701 gtgcttggtg aactttcttg taggtgcaaa ttctcccaac ctgtgtccaa ccatttcttc
     5761 tgtcggtcta acctcaatga aattttttcc gttgtgaaca ccaaaaagaa agccgatcat
     5821 ctccgggctt atctggctct ttcttgacca tgtcttaatc ataggagttg agtccggtct
     5881 tttgccttct attttcttta gcaactttgg ttccgtgaaa ggacccttct ttagcgatct
     5941 ggccatgttt tttgtgaatt cttaatgtgt tctaacaaaa gttttacaat ggcaataatc
     6001 ctagcagata ggcccccgct ctgtcaagca aggcgcttga gccctctccc tatcgaccgt
     6061 catcgcgagc gaagcgaagc gatccagaca tcaaccacca tcacgagatt gcttcgcttc
     6121 gctcgcaatg acaaccgtcg cccccactct atatccgata ttttaagccc ctttacaata
     6181 ccatcctttt ataatagtat gttgctatta tagaaacgtg tttaatcctt aattttaact
     6241 atcttggaca aaagagtcag aataaacaat caaatcagag ttcctgaagt cagagtaata
     6301 gacgatgatg gaacaaacct tggggtaatg cgagttgaag aagccataag gatggccgaa
     6361 tcaaaaaatc tggaccttgt cgaaatatcc cctaacgccg tcccgcctat tgcaaaaata
     6421 acggattatg gtaaatttca atacctagag aataagaaac agaaggcggc caaggccaaa
     6481 actcaggtca cggaggtcaa gaatatacag gtaagtatcg gaataagcga cagagatctg
     6541 gagttaaaag caaaaaacgc atcggaattt ttgaaagaag gaaacaaggt aagaatagtt
     6601 ttgacactaa ggggcagaga gaagtatttg aacaaagact tcttaaaaga aagagtcaat
     6661 cgacttttca aactcctaac ggaagaatac aagattatcg aggacttgaa gccggcgcag
     6721 agaggccaag cgatgacaat agagaggttg aagaaataac cccagataaa taagaatgtg
     6781 gccgagtgac tnnnnnnnnn naaataaatt aaaattaaat aattaggtcg cgaggttaat
     6841 ataagtttga tttcacgggg taaattataa aaaaatgaaa acaaataact catatctgaa
     6901 aagattgaaa gtaacaaaga atggtaaggt aatttccaga aaagccggaa agtgccattt
     6961 taatgcaaaa gaaagcaggt cgaaacagct tggcgcaagc aagatggttt cttttacgat
     7021 gaaaaaggcc gacatctcga gatatttgcc acacaatcag taattcaaat tgttaattcg
     7081 taatttaatc acatgcctag agtaaagaag gcggtacacg ccctaaaaag aagacgaaag
     7141 gttctgaagc aggctaaagg tttcagattc ggaagaggag caaagcagaa gcaggcacag
     7201 gaagcaatat tacacgctgg taaatacgct tttgcccaca gaaggaagaa aaagcgaaac
     7261 atgagaaagc tcttccagga aaagatcgga gccgcctcaa aagagaacgg aacctcctac
     7321 agcaaactta tgg
//
LOCUS       DAOTEX010000044         7078 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3118
            NODE_7581_length_7078_cov_4.085576, whole genome shotgun sequence.
ACCESSION   DAOTEX010000044 DAOTEX010000000
VERSION     DAOTEX010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868142
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 7078)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7078)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 883
            CDSs (total)                      :: 833
            Genes (coding)                    :: 829
            CDSs (with protein)               :: 829
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7078
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7581_length_7078_cov_4.085576"
                     /isolate="AMR_MDS_3118"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="P5056_03230"
     CDS             <1..469
                     /locus_tag="P5056_03230"
                     /inference="COORDINATES: protein motif:HMM:NF012863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HRY62753.1"
                     /translation="KFTILCIGDSHTFGKGTTNGNNYPKQLEKLLNSKTNNKFNVINR
                     AVPGQNSTQVLQNIRTQINVLHPDLIIILIGGGNLTNQYGFGSQINDFLYKFKVYKMF
                     EIILNNINKTAINTDAEKKIWEKEVYDTYKGSILERQRKEIKNSEEYKNALNI"
     gene            522..854
                     /gene="rnpA"
                     /locus_tag="P5056_03235"
     CDS             522..854
                     /gene="rnpA"
                     /locus_tag="P5056_03235"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HRY62754.1"
                     /translation="MEKKVTRSDFDMILKKGKNIYSPLLSFKYIFLDNSVSSRFSFVI
                     SGKVAKRAVDRNLFKRRGRHIVRKVKDDIQKGFIGAFFAKKGVINVSFIEMEKEVNSL
                     LKRAGVLL"
     gene            918..1640
                     /locus_tag="P5056_03240"
     CDS             918..1640
                     /locus_tag="P5056_03240"
                     /inference="COORDINATES: protein motif:HMM:NF014186.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HRY62755.1"
                     /translation="MFSFIFNIFYYPLYNAIIFLSALSPSNDFGLAVVALTILVKFLI
                     LPLSHVSIQTQRKLKEIEPEINRIKERHKDKQEEQAVKIMNLYKEHGVNPFASILLLF
                     IQLPMFIALYYIFRNTIDLASPVIFSFNILPTTINTMFLGVIDLSVSNIYLAILVGLT
                     QYIQTSLSLPEMPKEQAKNPTFADDFAKSMNWQVKYFLPLFIIFVAAKLNAAVSVFWI
                     TSNIFSIVHEFLVKRKASKIKA"
     gene            1702..1986
                     /locus_tag="P5056_03245"
     CDS             1702..1986
                     /locus_tag="P5056_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020252817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HRY62756.1"
                     /translation="MIKYYIYILASKPFGTLYVGVTNNIERRVYEHKRKDFSGFTKKY
                     GVNKLVYYEITDDINFAIKREKQLKKWNRDWKIKLIEDNNPKWEDLAEQV"
     gene            complement(2062..3381)
                     /locus_tag="P5056_03250"
     CDS             complement(2062..3381)
                     /locus_tag="P5056_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62757.1"
                     /translation="MSNKNYRILIIVISLVIVLAAVLTVFFLSRQAEVPVPAAVIPTT
                     TTTTQNNGVSFPTSGEVTSPATKNEPVSQNVIPEINPSNQAVTQVETFVGEIKNTADI
                     ATGGMTFIEKARNGIATTFIRYIEKGTGHIQEIAFDENIPTKVTNTTMTGIFSSVWNK
                     DGSAVIIKRLDSVNNNIQTIYGTIEDGTGDMTIPEGAVGSLRGTILPANTIDVAVSPL
                     NNKLFYLIKNGDSVIGSVSDFGGTNPFDKKINIWSSPISEWQVSWPKQDQIMLLTKPA
                     YNVSGLLYSSAVPKNNKFEKVLGDITGLTALTSVSGKKIIYSETTGSGFMTYILDKTT
                     GGTKVFPVKTLPEKCVWSRTNENVVYCGVPKVIVGSKYPDAWYKGNISFNDDIYRIDL
                     DVNSGRKIESKGQPDDLDATELKLSPNEDYLSFINKKDSKIWTLRLK"
     gene            complement(3385..3993)
                     /locus_tag="P5056_03255"
     CDS             complement(3385..3993)
                     /locus_tag="P5056_03255"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilin"
                     /protein_id="HRY62758.1"
                     /translation="MKPRTIQKIIFLLSLLILPSLTIAASYTLLEPIGGDQQTMIKVS
                     DPMTYLRSLYWWVVGLTIALSVLMLIVGGFQYTLSWADEKQKGEGRSRIENAITGLII
                     ALRAFLLLNTINPDLVSFEMSDAGSCKANNTPATTQSVPPTTGAPTGGGAVGGTGSPN
                     PAENVTPGTTNPEANIYNTQTTPGANIEAGTEVGPGGQPLSQ"
     gene            complement(3997..5001)
                     /locus_tag="P5056_03260"
     CDS             complement(3997..5001)
                     /locus_tag="P5056_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62759.1"
                     /translation="MLKNINKIILITALFLPSFGLAATLYPSYGGNVMFELNPKNPGA
                     NSSVTIKATSYSIDLTGSKISWYVGDKLEMEGNGKDSFSFKTGAVGKPITIKMVAEND
                     AKRVGRAISITPADIDAVWEANTYVPPSYRGKALAASKSSVKIVAIPQFKTENGTNIP
                     SSEIKYEWKKGNAVLLSGFGKNVLNTDVGSPLAGENIILLASDKSGTIVAEKKIEIVP
                     EDPEVVFYKENPAEGTNYNMALKGTIGLEGKEVSVRAEPFFFSYPSYSKGNNLLFSWK
                     VDGKGVNPNEGTPKIITLRNETGQDITSSISLSIKNAQKLFQETTSGFSLSSSAGFKF
                     "
     gene            complement(5011..5382)
                     /locus_tag="P5056_03265"
     CDS             complement(5011..5382)
                     /locus_tag="P5056_03265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62760.1"
                     /translation="MEDTQKKMTRTDWFILFQVAIIFDALGWVLALGIDEVLFGVPGV
                     LLNLIAGIVFLTFALLWRDKGIGNLSYFKSFGVGFLIEEIPILGTILPTWTAFIYKAR
                     SNYEQKNNPTSAMATVGKVTS"
     gene            complement(5419..>7078)
                     /locus_tag="P5056_03270"
     CDS             complement(5419..>7078)
                     /locus_tag="P5056_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HRY62761.1"
                     /translation="EKVARTFYVISYPRFLAENWFSPIINLDKIFDISIIVHPIDTSM
                     VLRNLQKKLAEVQSQIHANETKGLVRDPVLDTAYQDIEALRDQLSQARERVFDVGLYL
                     TIYAGSEEELNKVEAEIKTIMESRLVYVKPALFQQEEGFDSVMPMASDKLAVNTKLNS
                     SPLSSIFPFVSFDLTSNKGILYGVNRHNSSLIIFDRFSLENYNSVIFAKSGSGKSYFT
                     KLEILRSLMFDVDVIIIDPEREYEYLAEAVGGRFFNISLSSEHHINPFDLPFVREDES
                     PADVLRSHIINLVGLFRIMMGGMTPEEDAVMDNAIRETYALKDITADSDFSSAIPPLL
                     SDLELVLAGMEGGASLAQRLLKYTQGTWAGFLNRPTNVDINKKFVVFSVRDMEDELKP
                     VAMHIVMNNIWGSVRKELKKRMLIIDEAWWMMKSPDTASFMLSLAKRGRKYFLGLTTI
                     TQDVKDFLESPYGIPIITNSSMQILFKQSTASIDIVQKTFNLTDEEKYLLLESNVGEG
                     IFFAGLKHVAIKVIASYTEDQIITSDPSQLLAIKRSKEELKASE"
BASE COUNT         1982 a         1372 c         1391 g         2333 t
ORIGIN      
        1 taaatttaca attctttgca taggggattc tcatactttt ggaaaaggaa ctacaaatgg
       61 taataattac ccaaagcaac ttgaaaaact tttaaacagt aaaacaaaca ataagtttaa
      121 cgtaataaac agggctgttc ccggtcaaaa ttcaacgcag gtacttcaaa atatccgaac
      181 tcaaataaat gttttacatc ccgatttgat tataatttta ataggagggg gaaatcttac
      241 aaatcaatac ggctttggtt cacaaataaa tgatttttta tataaattta aggtttataa
      301 aatgtttgaa ataatattaa ataatattaa caaaactgca attaatactg atgccgagaa
      361 aaaaatatgg gagaaggaag tatacgatac atataaaggg agtattttgg aaaggcaaag
      421 gaaagagatt aaaaatagcg aagaatataa aaatgctctt aatatatagg tgtattgtgg
      481 catcagaaaa tttaggcgga taaattatat tcttttaata tatggagaaa aaggtaacta
      541 gaagcgattt tgatatgatt cttaaaaagg gaaagaatat ttactccccc cttctgtctt
      601 ttaagtatat ttttttggac aatagcgttt ccagcagatt ttcttttgta atatccggca
      661 aagtggccaa gagggccgtg gacaggaatt tgttcaaaag aagagggcga catatagtaa
      721 gaaaagtaaa agatgatatt cagaaaggtt ttatcggagc ttttttcgcc aagaagggcg
      781 taattaatgt ttcttttatt gaaatggaaa aagaggtaaa ttctttgtta aaaagagccg
      841 gggtgttact ataaaattag tctaaattag ttgaaagtgg gcttaattct ttattcataa
      901 ttcataattc tcgttttatg ttttcattca tttttaatat tttttattat cctctctaca
      961 acgccattat atttttgagc gccctttccc cttctaacga tttcggtttg gcggtagtgg
     1021 ctcttactat tctggttaag tttttaatac ttccactatc tcatgtttct attcagacac
     1081 agagaaaact taaagaaata gagccggaga tcaataggat aaaagaacgt cacaaagata
     1141 aacaagaaga gcaggcagtt aagataatga acctctataa agagcacggc gtaaatcctt
     1201 ttgccagtat tctgcttctt tttatacagc ttccgatgtt tattgccctt tattatattt
     1261 ttagaaatac aatagacctc gcttctcccg ttattttttc ttttaatatc ttgcccacca
     1321 ccataaacac tatgtttttg ggagttatag atctttctgt gtccaatatt tatttggcta
     1381 ttcttgtcgg actgacacag tatatccaaa caagtttatc tctccccgaa atgccgaaag
     1441 aacaggccaa aaaccctact tttgccgatg attttgcaaa aagtatgaat tggcaggtta
     1501 aatatttctt gcctctattt attatttttg ttgcggccaa gcttaacgcc gccgtctccg
     1561 ttttttggat aacaagcaat atcttcagca ttgttcacga gtttctggta aaaagaaagg
     1621 cctccaagat aaaagcttaa taatagtgga actattacct ctgttgtcat ccccgacctg
     1681 atcggggatc catctatttt aatgattaaa tattatattt atatactcgc aagtaaacca
     1741 ttcggtactt tgtatgttgg agtgactaat aatattgaga gaagagtata tgagcacaaa
     1801 agaaaggatt tttccgggtt cactaagaag tatggtgtaa acaaattagt atattatgaa
     1861 attaccgatg atattaattt tgcaataaaa agagaaaaac agttaaaaaa atggaataga
     1921 gattggaaaa ttaaattgat agaggataat aatcctaagt gggaagattt ggctgagcag
     1981 gtataactat ggatccccga tcaagtcggg gatgacattg ggagtggatc ttacgcgagc
     2041 cccattttta atttaaagtc gctatttcaa tcttagcgtc cagatcttgc tgtctttctt
     2101 gttgatgaat gataaatagt cttcattagg gcttaatttg agttctgttg cgtccaaatc
     2161 atccggctgg ccctttgact ctatttttct tccggagtta acgtcgaggt ctatacgata
     2221 aatatcgtca ttgaaagaaa tatttccctt ataccatgca tcaggatatt ttgatccgac
     2281 aataaccttt ggaacgccgc aataaacaac attttcattc gttctactcc agacgcattt
     2341 ctcaggaagt gtttttacag ggaatacctt tgttcctcct gtagttttgt cgagaatata
     2401 tgtcataaaa ccggaacctg tcgtttctga gtagattatt tttttaccgc taacgcttgt
     2461 aagagctgtt aatccagtaa tatctcctaa cactttttca aatttattgt ttttaggcac
     2521 cgccgatgag taaagcagac cggatacatt ataggcgggc tttgttagaa gcattatttg
     2581 gtcctgtttt ggccaagaaa cttgccactc tgatatagga gagctccaaa tgtttatttt
     2641 tttatcaaat ggattggttc cgccgaagtc tgaaacagat ccgataacgg agtcgccgtt
     2701 tttgatcaaa taaaacagtt tattgtttaa aggagaaaca gctacgtcta tagtatttgc
     2761 cggaagaatt gttcctctta aagaacctac agctccttcg ggaatagtca tgtcccctgt
     2821 tccgtcttct atcgttccgt atatagtttg aatattatta tttacactat ctagtctttt
     2881 tataatgaca gcagagccgt ctttattcca gacagagcta aatataccgg tcattgtggt
     2941 attggtgacc tttgtcggaa tattttcatc aaaagcgatc tcttgtatgt gtcctgtccc
     3001 cttttctata tatcttataa atgtcgtcgc aatgccgttt ctcgcttttt caataaaagt
     3061 catccctcct gttgcaatat ccgccgtgtt ttttatttct ccgacaaaag tttcaacttg
     3121 tgtgacggct tgatttgaag gatttatttc cggaattacg ttttgagaga ctggttcatt
     3181 ttttgtggcc ggagaagtga cctctcccga tgtcgggaaa gaaaccccat tgttttgggt
     3241 tgtcgtcgta gtggtaggga tcactgccgc agggacagga acttcagcct gtctagaaag
     3301 gaaaaagacg gttaaaacgg cagccaaaac gatgaccagt gatattacta ttattaggat
     3361 acgataattt ttgttgctca tattttattg acttagtggt tgtccgcccg gaccgacttc
     3421 agttcctgcc tctatgttgg cacctggtgt ggtttgagta ttataaatgt tcgcttccgg
     3481 attagttgtt cctggagtga cgttttcagc cggatttggc gagcctgttc ctcctacagc
     3541 acctcctcct gtgggcgcgc ctgttgttgg tgggacgctt tgggtcgtcg ccggagtatt
     3601 atttgcttta catgatccgg cgtcactcat ttcaaagctt acaagatcag ggtttatcgt
     3661 attaagtaaa aggaaagccc ttaacgcaat tattagacca gttatagcgt tttctatcct
     3721 gctccttccc tctccttttt gtttttcgtc cgcccagcta agggtgtatt ggaatccgcc
     3781 gacgataagc ataaggacag agagcgctat tgtcagtcct actacccacc agtagaggct
     3841 tcttaggtag gtcatggggt cgctcacttt tatcattgtt tgttgatcgc ccccaatcgg
     3901 ctccagaaga gtgtaacttg cggcaatggt aagagacgga agaattaaaa gggaaagcag
     3961 aaatattatt ttttgtattg ttcttggttt catattttaa aatttaaaac ctgcggaaga
     4021 acttaagcta aatccacttg tcgtttcttg gaataatttt tgcgcatttt ttatagatag
     4081 tgaaatagat gaggtgatgt cttgtcccgt ttcgttccta agagtgatta tttttggtgt
     4141 gccttcgttt ggattgaccc cttttccgtc aaccttccag ctaaacaaaa gattgtttcc
     4201 ttttgaatat gacggataag agaagaagaa aggttctgct cttacggaga cctcttttcc
     4261 ctctaatccg atagttcctt taagcgccat attataattt gttccttcgg ccggattttc
     4321 cttgtaaaaa actacttccg gatcttcggg aacgatctct attttctttt cggctacgat
     4381 cgtgccgctt ttgtctgagg ccaataaaat tatattctcc cctgcgagtg ggctgccgac
     4441 gtctgtattc aatacatttt taccaaaacc ggataaaagc acggcatttc cttttttcca
     4501 ctcatatttt atttcgcttg aaggaatatt tgtcccgttc tccgttttaa attgagggat
     4561 tgcgactatt ttaactgagc tttttgaagc cgctagcgcc ttccctctat aagaaggagg
     4621 gacatatgta tttgcttccc ataccgcatc aatgtccgcc ggggttattg atatggccct
     4681 tcctactctc ttggcgtcat tctccgcaac catctttata gttatcggtt tcccgaccgc
     4741 ccctgtctta aatgaaaaag agtcttttcc atttccctcc atttccaatt tgtctcctac
     4801 ataccatgag atttttgaac ctgttaaatc aatagagtag cttgtggctt ttattgtaac
     4861 agaagaattt gcccccgggt tcttagggtt tagctcaaac attacatttc ctccatagct
     4921 aggatataga gtcgcggcta gcccgaaaga aggtaaaaat agcgcggtga ttaatattat
     4981 tttgtttata ttttttaaca tcttttgttt ttatgatgtt accttgccaa ccgtggccat
     5041 tgcggaggtt ggattatttt tctgctcgta gttgcttcta gccttgtaaa taaaagctgt
     5101 ccatgtaggc aatatcgtgc cgagaatagg tatttcttct attaaaaaac cgacaccgaa
     5161 tgatttaaaa taacttaagt tgcctattcc tttgtctctc cataataatg caaaggtcag
     5221 aaatactatt ccggcgatta aattaagcaa tactcccgga acaccaaata aaacctcatc
     5281 tattccaagc gccagcaccc atcctagggc gtcaaaaata atggcaactt gaaaaagaat
     5341 aaaccaatct gttctcgtca tttttttctg tgtatcttcc atttttgaaa aattccaccc
     5401 tccccccaat accctaaact attcgctagc ttttaactcc tcttttgatc ttttaatggc
     5461 cagaagctgt gatgggtctg aagttatgat ttggtcttcc gtatatgaag cgatgacctt
     5521 gatagcaacg tgcttgagtc cggcaaagaa tattccctcc cctacgtttg attctaggag
     5581 cagatatttt tcctcatctg ttaagttaaa agtcttttga actatatcaa tagatgccgt
     5641 ggactgctta aagagtattt gcatagatga gtttgtgata attggaattc cataaggcga
     5701 ttccaagaag tctttcacgt cttgtgtaat agtggttagt cccaagaaat attttctccc
     5761 tctttttgca aggctcagca taaatgaagc ggtatcggga gacttcatca tccaccacgc
     5821 ctcatcgata ataagcattc tctttttgag ctccttcctg acagaccccc agatattgtt
     5881 cataacgatg tgcattgcga caggtttgag ctcgtcttcc atatctctga cggagaaaac
     5941 aacgaatttt ttgtttatat caacgtttgt gggtctgtta aggaagccgg cccatgttcc
     6001 ctgtgtgtat tttagaagtc tttgggcaag agacgcccct ccttccattc ccgcaaggac
     6061 aagttcaagg tcggaaagaa gcggcggtat ggctgaagaa aagtcggagt ctgcggtaat
     6121 gtctttaagg gcgtaggttt ctctgatggc gttgtccatt acagcatcct cttccggggt
     6181 cattcctccc atcattattc tgaatagtcc gacaaggttg atgatgtgtg atctcaaaac
     6241 gtccgctggc gattcatctt ctctgacgaa aggcaggtcg aacggattta tgtggtgttc
     6301 agaagaaagg gagatattaa agaacctacc ccctactgcc tccgctagat attcatattc
     6361 tctttcaggg tcaatgatta ttacatctac atcaaacata agagatctaa gtatctcgag
     6421 ttttgtgaag tatgattttc cggaaccgga cttggcgaag atgaccgagt tgtagttttc
     6481 tagagagaat ctatcaaaaa ttatcaaact ggagttgtgt ctgtttacgc cgtaaagaat
     6541 tcctttgttt gatgtaaggt caaacgatac gaacgggaaa atgctggata gaggagatga
     6601 atttaacttt gtgttgacgg ccagtttgtc tgaagccata ggcataacgc tgtcgaaccc
     6661 ttcttcctgt tggaaaagcg ccggtttaac atataccagt cttgattcca ttattgtttt
     6721 tatttccgcc tccaccttat tgagctcttc ttcagatccg gcatagatcg tgaggtataa
     6781 tccgacgtca aaaactcttt ccctggcttg tgacaactga tctctgagag cttcaatgtc
     6841 ctgataggcg gtatctagca cagggtctct gaccaatcct tttgtttcgt tggcatgtat
     6901 ctggctttgc acctccgcaa gctttttctg gagattccta agcaccatag aggtgtctat
     6961 cggatgcaca atgatagaaa tatcaaaaat tttgtccaag tttattatcg gagagaacca
     7021 attttccgcc aggaatcgcg gataggagat aacatagaac gttctggcca ccttctcc
//
LOCUS       DAOTEX010000045         6561 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3118
            NODE_8387_length_6561_cov_5.349524, whole genome shotgun sequence.
ACCESSION   DAOTEX010000045 DAOTEX010000000
VERSION     DAOTEX010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868142
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 6561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 883
            CDSs (total)                      :: 833
            Genes (coding)                    :: 829
            CDSs (with protein)               :: 829
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6561
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8387_length_6561_cov_5.349524"
                     /isolate="AMR_MDS_3118"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(27..221)
                     /locus_tag="P5056_03275"
     CDS             complement(27..221)
                     /locus_tag="P5056_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62762.1"
                     /translation="MSFEILIAHKRELEARLTRLTKELEKKDAQAAEDLLKIAVLQEE
                     NAKLQAEVAKYKATSVPFHE"
     gene            220..684
                     /locus_tag="P5056_03280"
     CDS             220..684
                     /locus_tag="P5056_03280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62763.1"
                     /translation="MEHHLSLLGPAFRLGVLVIILSFGATYVCPDMLFDDLVPGVSFA
                     LGHGAEVALFVEFHTEKVPTFLEELVFFLQRVHCRLGDEDFDDDLTSISLFEQFEPAF
                     LGDTFAGEGILEAFAFEKTLFLAMGDFEELHIATFSFNVQIILSFSSIAYFS"
     gene            complement(826..1302)
                     /locus_tag="P5056_03285"
     CDS             complement(826..1302)
                     /locus_tag="P5056_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62764.1"
                     /translation="MTTPCKPLILVIALLIGLLFINDYFCKKSPAQVQAELQLRQIAS
                     KAHTAEDYRKIWAVYKDSPLAETAFFLAADWLIARINCATSGGEIDEILNYHCTHEGV
                     LLVELKYQEKARSLYSPWLRAKMKTKIEQIEMHAKERMHQFAVKELEKELKVDEAK"
     gene            1460..4957
                     /locus_tag="P5056_03290"
     CDS             1460..4957
                     /locus_tag="P5056_03290"
                     /inference="COORDINATES: protein motif:HMM:NF012361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRY62765.1"
                     /translation="MDTSENNKKKPETAPEKEERILKFWTESEIFQKSLKKDAPEGEY
                     VFYDGPPFATGLPHYGHILGGTMKDAVPKYKTMRGYRVPRNWGWDCHGLPIENLIEKE
                     LGLQTKKDIETLGVGKFNEAARASVFRYKREWQVTVPRTGRFVDMENDYKTMDPSFTE
                     SVWWVFKNLYDKGLVYFGHKTMHICPRCETTLANFEVSQGYKDITDISLTAKFELVDE
                     PGTFVLAWTTTPWTLPGNVALAVGPEIDYVKIEKDGAKLILAKDRLSAVGEGYKILEE
                     FKGAKLVGKSYKPLFDYYAKKADLANKENGWKICPASFVTTQDGVGVVHIAPAFGEDD
                     NKLGRQYNLPFVQHVGMDGRFRPEVTDFADISVKPKDDHQATDVLIIKNLAARGLLFS
                     KEKIIHSYPHCWRCDTPLLNYAASSWFVDIQKIKSKIVENVKKSNWVPQNIRDKRFIN
                     LIEDAPDWNVSRSRYWGAPLPIWKCDKCDEKKVVGSVDELKKGMNDSDNTYFAMRHGE
                     SESVANRTISSSKYHHENPLTSLGREQVKVSAEKLKSENIDLIISSPYRRTTETAKIV
                     AVELGIPETEIVFDDRIVELQVGDLDGGLVEDYQKYFANFEERFYKNPPNGENHVDTK
                     NRMMSAMFDIDKKYSNRRILVISHGDPLITLMGGSKGLTPKEIAGRHDEFHFKPAEFR
                     KLDFAPFPHNENYELDLHKHFIDDISFKCSCGGEMKRVPEVFDCWFESGAMPYGQSHY
                     PFENKEEFERNFPAQFIAEGIDQTRGWFYSLMVLSTALFDKPAFLNVIVNGTVLAEDG
                     QKMSKRLKNYPEVHQIMDKYGADSLRYYLLSSPAVRAEDMRFSERGVDEVYKKIILRL
                     YNVYSFLEMYDRSEKGKAKSEKQEDSKNVLDKWIVARLSEVTKEITDAMERYELDRAT
                     RPIDGFVDDLSTWYLRRSRDRFKGDDIADKEAALATLRYVIKTLAKLMAPFMSFISEE
                     LYQKVKDATDPESVHLDSWPEIENDELRIKNYEKVLSDMGAVRKIVSLGLELRAKANI
                     KVRQPLQELRIRNKELGEEYLPLIEDELNVKNVIVGVVQDEEVALDTKATPELKEEGS
                     LRELVRNLQDMRKKMGLNPQDKVLLRISTDAKGKEFVKKFETELKKSAGLSGLNIEDG
                     VIGSEIALEDLKFMVSLVK"
     gene            4961..5518
                     /locus_tag="P5056_03295"
     CDS             4961..5518
                     /locus_tag="P5056_03295"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRY62766.1"
                     /translation="MEELSQQEKPKIEIALARPEDAWGTKNVFYQTWLTTYPNKEVGV
                     TVEDVEDRYKDRFSEKELARRKDRLANPPSNEIFLVAKEGDEVVGACVLTKRGNFNQL
                     QAIYVLPEYQGRGIGKMLWSEALNFFGTEKDIVVEVATYNTNAIEFYKKLGFQDTGKR
                     WKDEYTRMKSGAIIPEMEMILKARK"
     gene            5673..6263
                     /gene="recO"
                     /locus_tag="P5056_03300"
     CDS             5673..6263
                     /gene="recO"
                     /locus_tag="P5056_03300"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HRY62767.1"
                     /translation="MSYHIYKTEGFVVGSVPVGEANRLISVYTEELGLVRGMAQGVRN
                     LRSKLKHSLQDLFYTKVSFVRGRDMWRIVNAERLTLVGDELLKSDKKIFLAKLALLIR
                     RFVKGEGRDEALFNNLRSGLAFLKDNELTEAEMKKFELIFVLRFFDQLGYGPYESHFG
                     EILTFSLWSRDALALADGKESQILAVINRAMQESHL"
BASE COUNT         1868 a         1259 c         1670 g         1764 t
ORIGIN      
        1 ttgggccttg tcttgcgaca gggccgttac tcgtgaaagg ggaccgacgt ggccttgtat
       61 ttcgcgacct cggcctggag ctttgcgttc tcctcttgga gcacggcgat cttgaggagg
      121 tcctcggcgg cctgcgcgtc cttcttctcg agctccttgg tgaggcgcgt gagtctcgct
      181 tcgagttcgc gtttgtgcgc gatgaggatt tcaaacgaca tggagcacca tctttccttg
      241 ttgggcccgg cctttcggct tggcgtgctg gtgattattt tatctttcgg tgcgacttat
      301 gtgtgccctg atatgctttt tgatgatctc gttcctggcg tctcgttcgc gctcggccat
      361 ggtgcggagg tcgcgctctt cgtcgagttt cacacggaaa aggtccccac gtttttggag
      421 gaacttgttt tcttccttca aagagttcat tgccgactcg gggacgaaga cttcgacgac
      481 gatctcacct cgatctctct gttcgagcag ttcgagccgg catttctcgg tgacaccttc
      541 gcgggtgagg gcattctcga ggctttcgcc ttcgagaaaa cgctttttct cgccatgggt
      601 gattttgaag agcttcatat cgccaccttt tctttcaatg tgcagattat attgtctttt
      661 tcaagtatag catattttag ttaaaaagtc aagagaaaac aaaaaattca ccctgttaag
      721 taaaaatttc tctttaatat aaagagaaat tttaaatttt ataaataaaa ttttactaaa
      781 cagggtaaaa aagccgccta ccctaatagg gtggcggcgg acggcttatt tggcctcgtc
      841 gactttgagc tccttttcga gctcttttac ggcgaattgg tgcattcttt cttttgcgtg
      901 catttcgatc tgttctatct ttgtcttcat ttttgctctc agccaaggag aatataaaga
      961 gcgagctttc tcttggtact ttaattctac aaggaggacc ccctcatgtg tgcaatgata
     1021 gtttaagatt tcatcgatct ctccgccact tgtggcgcag ttgattctgg cgatgagcca
     1081 gtcggcggcg aggaagaacg cggtctctgc gagcggggag tctttgtaga cagcccagat
     1141 cttcctgtag tcctccgccg tgtgcgcttt agaagcaatc tggcggagtt gtaattctgc
     1201 ttgaacttgt gcgggtgatt ttttgcagaa gtaatcgttg ataaacagaa ggccaatcag
     1261 taaggcaatt acaaggatca agggcttgca gggtgtcgtc atttcatttc ctccctttag
     1321 atttagaaag tgaatgtgtt ttttgtaccc aacttgtcaa tgtcctagtt tcgaacatca
     1381 tactatagca atttgtcgtc ttgtcaaatc gtgctttttg ccctttaaga agtctctttt
     1441 ctgtgatagc atatttttta tggacacatc tgaaaataat aagaaaaaac ccgagacggc
     1501 ccctgaaaaa gaggagcgaa ttttgaaatt ttggacagaa agtgaaattt tccagaagtc
     1561 gttaaagaaa gatgcgcccg agggggaata tgtcttctat gacggaccgc ctttcgctac
     1621 aggattgcca cactacggac atatcttggg cggcacgatg aaagatgcag ttccgaaata
     1681 caagacgatg cgcggatacc gcgttcctag gaattgggga tgggattgcc acggtcttcc
     1741 tatagaaaat ctgatcgaaa aagagcttgg gctacagaca aaaaaagata ttgagacgct
     1801 tggtgtagga aagtttaacg aagccgcgag ggcgagtgtt ttcaggtata agagagagtg
     1861 gcaggtgaca gtgccaagga cggggcgttt cgtggatatg gagaatgact acaagacgat
     1921 ggacccttcg ttcacggaat ccgtctggtg ggttttcaaa aatctctatg acaaagggct
     1981 tgtatatttc ggacataaga cgatgcatat ttgtccgcga tgcgagacaa cactcgcgaa
     2041 cttcgaggta agccaaggat acaaagacat tacagacata tcactcactg cgaaatttga
     2101 actcgtcgac gaaccgggaa cttttgttct tgcgtggacg actactcctt ggacgcttcc
     2161 gggaaatgtc gcgctcgctg tcggtccgga aattgattat gtgaaaatag aaaaagacgg
     2221 ggctaaatta attttggcca aagacaggct ttccgccgta ggagagggat ataagatact
     2281 ggaagagttt aagggggcca agcttgtcgg caagagctat aagcctcttt ttgattatta
     2341 tgccaagaaa gcagaccttg cgaacaagga gaacggctgg aaaatatgtc ccgcgagctt
     2401 tgttacaaca caagacggtg tcggcgtagt gcacattgct ccggctttcg gtgaagacga
     2461 caacaagttg ggtaggcagt ataatttgcc gtttgttcag cacgtcggta tggacgggcg
     2521 atttaggcct gaagttacgg attttgccga tatttctgtt aaaccaaagg acgaccatca
     2581 ggctacggat gtccttataa tcaaaaacct cgccgcgcgc ggactacttt tctcaaaaga
     2641 aaagataatt cactcgtatc ctcattgctg gagatgcgac actccgcttc ttaactatgc
     2701 ggcctcttcc tggtttgtag atattcagaa aataaaatcc aagattgtcg agaatgtgaa
     2761 gaagtctaat tgggtgcctc agaatatccg cgacaagcga ttcataaacc tcatagaaga
     2821 tgctcccgac tggaacgttt cccgctcgag gtattggggc gcgccgcttc ctatttggaa
     2881 gtgcgacaaa tgcgacgaaa agaaagtcgt cggctcggta gatgaattga agaaggggat
     2941 gaatgattcc gacaatactt attttgcaat gcgacacggc gagtcggagt ctgttgcgaa
     3001 cagaacgata agctcgtcta aatatcatca tgagaatccg ctcacaagcc tcggcaggga
     3061 gcaggtgaag gtatcggcgg aaaaattaaa atctgaaaac atagatctta taatctcttc
     3121 accatacaga aggacgacag aaacagcgaa gatagttgcg gtcgagcttg gcataccgga
     3181 gacagagatc gtttttgacg acagaattgt cgagcttcag gttggagatc ttgacggagg
     3241 tctcgtagaa gactaccaga aatattttgc taattttgag gagagatttt acaagaaccc
     3301 tcctaacgga gagaatcacg tagacactaa gaacaggatg atgtctgcga tgtttgatat
     3361 tgataagaaa tattcgaaca gaagaatact cgtaatcagt cacggtgatc cgcttataac
     3421 gcttatggga ggctcaaagg ggctcacccc aaaagagatt gccggaagac atgacgaatt
     3481 tcacttcaag cccgcagagt tcaggaagct tgattttgcg cctttccctc ataatgaaaa
     3541 ttacgagctg gatctgcaca agcattttat agatgatata agcttcaaat gttcttgcgg
     3601 gggcgagatg aagagagtgc ctgaggtttt tgactgctgg ttcgagtccg gcgcgatgcc
     3661 atacggacaa tctcactatc cgttcgagaa caaggaagag ttcgagagaa attttccggc
     3721 gcaattcatt gctgaaggta ttgatcagac gagaggctgg ttctattccc tcatggttct
     3781 ttcgaccgcg ctttttgata agccggcttt tttgaatgtc attgtgaatg gtactgttct
     3841 ggcggaagat ggccagaaaa tgtcgaagag acttaagaat tatcccgagg ttcatcaaat
     3901 aatggataaa tatggtgctg actcattaag atattattta ctctcttctc ctgctgttag
     3961 agctgaagat atgcgtttct ccgaaagagg agtagacgag gtgtacaaga agataatttt
     4021 gcgcctatac aatgtttatt ctttcttgga gatgtatgat aggagcgaaa aaggaaaagc
     4081 gaaaagtgaa aaacaagaag actcgaaaaa tgtcctcgac aagtggattg ttgcaagact
     4141 gagtgaagta acgaaagaaa taaccgatgc gatggagaga tatgagctcg atagggccac
     4201 aaggccgata gacggattcg tagatgacct atctacatgg tatttgaggc gttcacgaga
     4261 caggtttaag ggggatgata tcgctgacaa ggaggccgcc ctcgcaacgc ttcgatatgt
     4321 aataaagacg ctcgcgaaac ttatggcacc gtttatgtca ttcatctctg aggagcttta
     4381 tcagaaagtg aaagatgcga ccgatccgga gagcgttcat ttggattctt ggccggaaat
     4441 agagaatgac gaattaagaa ttaagaatta cgaaaaggtc ttgtctgaca tgggtgccgt
     4501 gagaaagata gtgtccctgg ggttggagct gagggcgaag gcgaatatta aggtgcgtca
     4561 gccgcttcaa gaattaagaa ttaggaataa agaattagga gaagaatatt tgccactcat
     4621 cgaagatgag ctgaacgtta aaaacgtcat tgtgggcgtt gttcaagatg aagaagttgc
     4681 tttggatact aaggccactc ctgaacttaa agaggagggg tctttgcgcg agcttgtaag
     4741 aaatcttcag gatatgagga agaaaatggg cttaaatccg caggacaaag tgcttcttag
     4801 aatatctacg gacgcaaaag gcaaagaatt cgtgaaaaaa tttgaaactg aactcaaaaa
     4861 gtccgcaggt ctttccggtc taaacataga ggatggtgtt atcggctctg aaatagcact
     4921 ggaagattta aaattcatgg tttctctcgt gaaatagttt atggaagaat tatcacaaca
     4981 ggagaagcct aaaatagaaa tcgcgcttgc gagacctgag gacgcatggg gaacaaagaa
     5041 cgttttttat caaacatggc tgacgacata cccgaataaa gaggtgggtg ttaccgtaga
     5101 ggacgtcgaa gaccgctata aagatagatt ttcagaaaaa gaacttgcca gacgaaagga
     5161 tcgattggcg aacccgcctt caaacgaaat atttttagtg gcaaaagagg gcgatgaggt
     5221 tgtcggtgct tgtgtgttga ccaagagagg taattttaat cagctccagg caatttatgt
     5281 tttgccagaa tatcagggca gaggtattgg taaaatgttg tggtctgagg ctttgaattt
     5341 ttttggaact gagaaagata ttgttgtaga agttgcgaca tacaacacaa atgcaattga
     5401 gttttataaa aagcttggtt tccaagatac gggtaagagg tggaaggacg aatatactag
     5461 gatgaaaagt ggagcgatca ttcctgaaat ggaaatgata ctaaaagcga ggaaatagta
     5521 ctggaagatt taaaattcac tgtcttgctc acgaaatagt agttcaccgt cattgcgagc
     5581 gcagcgaagc aatccaggaa ctttagattg ccacgagctt cagctaggac gcctcgctct
     5641 cgcaatgaca ataatgagaa tacactatta aaatgtctta tcacatatac aaaaccgaag
     5701 ggtttgttgt tggctctgtg ccggtcggag aggcgaacag gcttatttcc gtttataccg
     5761 aagagcttgg actcgtccgc ggtatggcgc agggtgtgcg caacttgcgg tcaaaactga
     5821 aacactctct tcaggatctt ttctatacaa aagtttcttt tgtgcgcgga cgagacatgt
     5881 ggcgtattgt gaatgcggaa aggctcacgc ttgtcggtga cgaattgctt aagagcgaca
     5941 aaaagatctt cttggcgaag ctggcgctac tcatcaggcg gtttgttaag ggagagggga
     6001 gggacgaggc gctttttaat aacttgcgct cgggtctcgc ttttcttaag gataatgagc
     6061 tcactgaagc tgaaatgaaa aagtttgagc ttatttttgt tttaagattt tttgatcaat
     6121 tgggctacgg tccatacgaa agccactttg gcgagatact cacattctct ctctggtctc
     6181 gcgacgcact tgctttagca gatggcaaag aaagccaaat cctcgcggtc attaatagag
     6241 caatgcaaga gagccatctt taatagatat tagaaggtag atagtagaaa gtagttagaa
     6301 caaagaacgc gtttttatta tgcgcaaata acttagcggt cgctaagtta tttgatacac
     6361 ccgatggggg ggcaaccccc ctacacaaat aatttcgcgg tcgcgaaatt atttgtgtag
     6421 gggctttttt aattaacagg gtgaacacaa gctattgagc cataacggaa tagttgacaa
     6481 aattgtaaaa atatgtatat aatgtgtcga atttgtatat tgaaaattca ataaaaggag
     6541 ggttagaaaa atgcctaaaa t
//
LOCUS       DAOTEX010000046         6499 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3118
            NODE_8507_length_6499_cov_4.470050, whole genome shotgun sequence.
ACCESSION   DAOTEX010000046 DAOTEX010000000
VERSION     DAOTEX010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868142
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 6499)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6499)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 883
            CDSs (total)                      :: 833
            Genes (coding)                    :: 829
            CDSs (with protein)               :: 829
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6499
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8507_length_6499_cov_4.470050"
                     /isolate="AMR_MDS_3118"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2135
                     /locus_tag="P5056_03305"
     CDS             <1..2135
                     /locus_tag="P5056_03305"
                     /inference="COORDINATES: protein motif:HMM:NF024487.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AAA domain-containing protein"
                     /protein_id="HRY62768.1"
                     /translation="IAEGRPQVFNLKEIIEKYPKTTKKVGINAEQFTTIVSNAVIDVP
                     ELEFDKQIRYLNLKQKVSKDFSNIHELNYAARMKQIEDLVTTQVTYLLDGRLIDFYEH
                     NRSDAQTLRNIIKNKQRFPRDEFMKLKEAFPCILAGIRDYAEYIPLESGLFDLVVIDE
                     ASQVSIAQAFPALLRAKKVLILGDKKQFSNIKAAQARGDTNREYLNGLNVSFRDNVSS
                     DAVKLVRLDKFNIKTSILEFFEFISNYNIQLLKHFRGYKEIISYSNKYFYQESLQVMK
                     IRGKSIDDVVKFSNVESTAADELYPNTNKKEVEFIISELKRLKDEDKALSVGIITPHT
                     NQQKLLVEMIGKMPEWDYFQNKLQLKIMTFDTCQGEERDIIYYSMVASPHSDKLWGVF
                     IKDLSNVDIEEDGQIKAQRLNVGFSRAKECAHFVLSQPTDSFTGSVGEALRHYQYVLE
                     EARKEHGVSEVDEKSKMEPEVLNWFYQTKFWKQHKDTIEFMPQFEIGKYLKQLDRTYT
                     HPAYKVDFLLIYTDERHKEHKIIIEYDGFREHFKDIDEINEFNYENYYTDEDVYRQKV
                     LESYGYKFLRINKFNIGENPIASLDDKIGCLLKNPENTNSLLANIHGTIEGLQNGEMR
                     ECPKCKNVKSVKDFKDTSLITGYGRFCNECKNHRTYYSSPSTPKPAPVLSDQTCPRCG
                     NRMILRNGRRGKFYGCSRFPYCKGTRNA"
     gene            2142..3728
                     /locus_tag="P5056_03310"
     CDS             2142..3728
                     /locus_tag="P5056_03310"
                     /inference="COORDINATES: protein motif:HMM:NF022525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2779 domain-containing protein"
                     /protein_id="HRY62769.1"
                     /translation="MSLLTKTDFILYRECPNNVWVKRHKPDEYAKFEVSEFEQSLAVM
                     GNEVEELARGMFPTGYLVERRSEGARELTKKLIDERTPVIFQAVFATEKYFAAADVLQ
                     WNELAQKYDIYEIKMSSTEEDDEDEEEGKPKRVNKKRELQYEYDLAFQTNVIEGCGIQ
                     LNKKYLVRLNRQYVRIGELNFTPGQLFIVEDKTEAIGRLSPTAAIEMSQAQTYLSATK
                     MPAAPCPCYYKGRSSHCTAFSYINPHVPTYSVHDLNRIGNSKRYLKELLDEGILEIGD
                     VPIDDRLKSKPPKAGAKPSKPRKLNQILVHKSQEPLVNLESLKAELDSLTFPLYFLDY
                     ETYPTAIPPYSGYHPYQQIVFQYSLHVLKNKDSEPAHFECLILDGEPSERIAKSLCEH
                     IGDTGTVISWYKTFENSRNKELADFLPSHKDFFLNVVSRTYDLMDIVENQHYVHPGFQ
                     GRSSIKKVLPVLAPHLSYKVLGVQSGTDAIEAYRKIIKGEIKEDAIKEKESEMLEYCK
                     LDTYAMYVLWRFFSNVIKET"
     gene            3731..4981
                     /gene="rmuC"
                     /locus_tag="P5056_03315"
     CDS             3731..4981
                     /gene="rmuC"
                     /locus_tag="P5056_03315"
                     /inference="COORDINATES: protein motif:HMM:NF014680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HRY62770.1"
                     /translation="MTYLIFILAISGAGVVGFYIASFIKGKELALKNKELVVKDQEIA
                     IEKRALEERSKDIEQLEARFKAVASDVMRVHSKDFLEEFEKARKVHNTDFETKEKGIE
                     RIVEGLSKSMETVDKKMVEFEKQRVEQAGALGESIKNVLDTGAKMQEAALSLKTVLSS
                     ASAVRGRWGEAVLKNLLEQSGLTEGIDFQMQETIAGDEIALRPDVIVNLPGGLQLAID
                     SKAGLEEFFKAVEEKDVQKKDEHIKKFAQNLRGHIKDLSSKEYQKHLDVRIPYVIMFI
                     PGEAAVRAVFEQDLELYREAQEKKVMLASPATIMPLVLLIAHAWKQQKTVENASKLAK
                     EITDLGGRLKTFLGHVVGIGSSLSNATKKFNDAASSWDTRVFPKIEQINTLGGNMQVE
                     GEMAQVEIEPRAPGKLLPPSKEEK"
     gene            4990..5313
                     /locus_tag="P5056_03320"
     CDS             4990..5313
                     /locus_tag="P5056_03320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62771.1"
                     /translation="MLSQKAIDDFKKLYKEHYGEELNDFVASEAANRLIRMMDLVYRP
                     IPKAWEEKYNEIKKEQEAIPENKKTDFVKHLVSDAKVSEFREKIGDKKFWELVTEANR
                     KNKKD"
     gene            complement(5310..5978)
                     /locus_tag="P5056_03325"
     CDS             complement(5310..5978)
                     /locus_tag="P5056_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62772.1"
                     /translation="MTDHNIPIQTSNTKKFSFYENFDGHYHKINEIRDLIPQFKAFYY
                     AEKSKEPGRSAIKIINDFNAQIAPLTFFPWEKQYRLWRKKWDTELLALEGYQEQQKEV
                     RQLIKVRNEQNALVVPADETLEDGAKTLAGELLNDAMNILKKDQDEEELYEDDIIVKR
                     RNYVLNVYNYITRSAHSKEALRLKSNADKRETMGFLMDLMSLATAGKMTDEDLALLKN
                     SSKS"
     gene            6299..>6499
                     /locus_tag="P5056_03330"
     CDS             6299..>6499
                     /locus_tag="P5056_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62773.1"
                     /translation="MLTKLLNKKYFLFRFIILFLVCGFGFANNAGAEVAFVQSKAVDS
                     WNLSSFSVTFDSSVTAGNIIAVS"
BASE COUNT         2100 a         1173 c         1423 g         1803 t
ORIGIN      
        1 ctattgccga aggtcgtccc caggttttta atcttaaaga aattatcgag aaatatccga
       61 agacaacaaa gaaggtcgga ataaacgccg aacaatttac caccatagta agcaacgccg
      121 tcatagatgt tcccgaatta gagttcgaca aacagattag atatttgaac ttaaagcaaa
      181 aagtaagcaa agacttttct aatatccacg agctgaatta tgcggcgcga atgaagcaga
      241 ttgaagattt agtaacaacc caagtaacat accttcttga cggacgactc atagatttct
      301 atgagcataa ccgaagcgat gcccaaacgt tgcgcaatat cattaagaat aaacaaaggt
      361 ttccgcgaga tgagtttatg aagcttaaag aagctttccc gtgtattttg gctggcatca
      421 gagactatgc agagtacatc ccgttggagt ctggattatt tgatttggtg gtaattgatg
      481 aagcgtcgca ggtaagcatc gcccaggcgt tcccagcgct tttacgcgcc aagaaagtat
      541 tgattcttgg agacaagaag cagtttagta acatcaaagc tgcgcaagcg cgaggcgaca
      601 ccaaccgcga atatcttaat ggattgaatg ttagctttcg agataacgtg tcatctgatg
      661 cggtaaagtt agtgaggctt gataagttta atatcaaaac ttctattttg gagttctttg
      721 agtttatcag caattacaac atccaactgt tgaaacattt taggggttat aaagaaataa
      781 tttcttactc taataagtat ttttaccaag agagtttgca agtaatgaag attcgtggaa
      841 aatctattga tgatgtggtt aagttttcta atgtcgaatc aacagctgcc gacgaattgt
      901 atcccaacac caacaaaaaa gaggttgagt ttattatttc agaactcaaa cgcttgaagg
      961 acgaggataa agcattaagc gttggtatca ttacaccaca tacaaatcaa cagaaattat
     1021 tggttgaaat gataggtaaa atgccagagt gggattattt ccaaaacaaa ctacaactaa
     1081 aaataatgac cttcgatacc tgccaaggtg aagagcgcga catcatctat tattcaatgg
     1141 tcgcttctcc acatagcgac aagctatggg gtgtttttat taaagattta agtaatgttg
     1201 atattgaaga ggacggacag ataaaagcgc agcgcttaaa tgttggtttt agccgtgcga
     1261 aagaatgtgc ccactttgtt ttgagccaac ccactgatag ctttactggt tctgtcggag
     1321 aagctttgag acattatcaa tatgttttag aagaagcgag aaaagaacat ggtgtatccg
     1381 aagtcgatga gaaatccaag atggagccag aggtattgaa ttggttttat cagacaaagt
     1441 tctggaaaca acacaaagat actattgagt ttatgcccca gtttgagata ggaaaatatc
     1501 ttaaacaatt agaccgcaca tacacgcacc cagcatataa agttgatttc ttgcttatct
     1561 ataccgatga gcgccacaag gagcataaaa ttatcattga atacgatggt ttccgagaac
     1621 actttaagga tatagacgaa atcaatgagt tcaactacga aaattattac accgatgagg
     1681 acgtttatcg ccagaaagta ttggaaagct acggctataa gtttttgcga atcaacaagt
     1741 ttaatatcgg ggaaaacccg atagcaagtc ttgatgataa aattggatgc ctacttaaaa
     1801 acccagagaa taccaattcg ttgcttgcaa atatacacgg cactattgag gggctgcaaa
     1861 atggggagat gcgggagtgt cctaaatgca agaacgtaaa atctgttaag gattttaagg
     1921 acacctcttt gataactggc tatggtagat tctgtaatga gtgcaagaat cataggactt
     1981 attattcttc gccatctacg ccgaagccag cgcctgtgtt atcagaccag acttgccccc
     2041 gctgtggaaa tagaatgata ttaaggaacg gacgcagagg taagttttat ggctgttcaa
     2101 gattccctta ctgtaaggga acaagaaatg cttaaacttt tatgtcttta cttacaaaaa
     2161 cagactttat tttatatcga gagtgcccaa ataatgtttg ggtaaagaga cataaacccg
     2221 acgaatatgc caagtttgaa gtttcggagt ttgaacaatc tctcgcagtg atgggtaatg
     2281 aagtggaaga attagcacga ggaatgtttc caacgggata ccttgttgag cgtcgcagcg
     2341 agggagcacg ggaactaaca aaaaagctta ttgacgagcg tacgccagtc atttttcagg
     2401 cggtattcgc aactgaaaaa tattttgccg ccgcggacgt tcttcaatgg aatgaattag
     2461 cacagaaata tgacatatac gaaataaaga tgtcttcaac agaggaagat gacgaagatg
     2521 aggaggaagg aaagcctaaa agagtcaaca aaaaaagaga acttcaatat gaatatgacc
     2581 tcgcttttca aaccaacgtc attgagggtt gcggcataca gctaaacaaa aaatatttag
     2641 tccgtctaaa cagacaatac gtgaggatag gcgaacttaa tttcacacca ggacagcttt
     2701 ttattgtcga ggacaagacg gaagctatag gtcgtttgag tcccactgcc gcgatagaaa
     2761 tgtctcaagc tcaaacttat ctgtccgcta caaaaatgcc agcagcgccg tgcccttgtt
     2821 actacaaagg acgcagttcc cactgtacgg cattttctta cattaaccca cacgtaccta
     2881 catacagtgt tcacgacttg aatagaattg gaaatagcaa aaggtacctc aaagagcttc
     2941 ttgatgaagg tattttagaa atcggtgatg tgccgataga tgacagactt aagtcaaaac
     3001 cgccgaaagc aggtgcaaaa ccaagtaagc cacggaagct gaatcaaatc ttggttcaca
     3061 agtctcaaga accgctagtg aatcttgagt ctctaaaggc tgaacttgat tcgcttactt
     3121 ttccacttta ctttctcgac tatgagacat atcccacagc aatcccgcct tacagcgggt
     3181 atcatccata tcagcagatt gttttccagt attcgctgca cgtcttaaaa aataaagatt
     3241 cggaaccagc tcattttgag tgtttgatat tagatggaga accatctgaa cgtatagcga
     3301 agagtttgtg cgaacatatt ggcgatactg ggacagttat atcttggtat aaaacttttg
     3361 aaaactcacg aaataaagag ttagcagatt tcttgccaag tcataaggac ttcttcctca
     3421 atgtagtttc ccgtacgtat gacttaatgg atattgttga aaaccaacat tatgtacacc
     3481 caggatttca gggtaggtca tcaatcaaga aagtgctccc agtgttagcg ccacacctgt
     3541 cttataaggt acttggcgtc caaagtggca cagacgccat agaggcttat aggaaaatta
     3601 tcaaaggaga aataaaagaa gatgctatta aagaaaagga gagcgagatg cttgaatact
     3661 gcaaacttga tacttatgcg atgtatgtac tatggagatt ctttagtaat gtaataaagg
     3721 aaacgtaatt atgacatatc taatttttat acttgctatt agtggtgctg gagtggttgg
     3781 tttttatatt gcttcattca taaagggtaa agagcttgct ttgaagaata aagaattagt
     3841 agtgaaagac caagaaattg ctattgaaaa acgtgcgctc gaagagagaa gcaaggacat
     3901 agaacagctt gaggctcgtt ttaaggctgt tgccagtgat gttatgagag tccactctaa
     3961 agattttttg gaagagttcg aaaaagcccg aaaggttcac aatactgatt ttgaaaccaa
     4021 agagaaaggc attgagcgca ttgtcgaggg tttgagtaaa tcaatggaga ctgttgataa
     4081 aaagatggtt gagtttgaaa agcagagagt agagcaggcg ggtgcgttgg gagaaagtat
     4141 aaaaaatgtt ttagacacgg gagcaaaaat gcaggaagca gccctttctc ttaaaacagt
     4201 tttatcatct gcgagcgcag ttcgcgggcg ctggggcgaa gctgttttga aaaatctact
     4261 tgagcagtca gggcttacag aaggtattga ttttcaaatg caggaaacaa tcgcgggaga
     4321 tgagattgca ctacgacccg atgtaatagt caacctccct gggggtctac agctggcgat
     4381 tgatagtaag gctgggcttg aagagttttt caaagctgtg gaggagaaag atgttcagaa
     4441 aaaggatgaa cacataaaaa agtttgccca aaatctccga ggacatatta aagacctttc
     4501 gagcaaggaa tatcagaaac accttgatgt gaggattcct tatgtgatta tgtttattcc
     4561 aggagaagca gcagtgcgtg ccgtatttga acaagacctt gaactttata gagaagctca
     4621 agaaaaaaag gttatgcttg caagtcctgc aacaataatg ccgctagtct tattgattgc
     4681 tcacgcctgg aagcaacaga agacggtcga gaacgcttca aaattagcga aggaaattac
     4741 tgatttggga ggaagattaa aaactttctt gggacacgtc gtaggtatcg gttcatcttt
     4801 atcaaatgct acaaaaaagt ttaatgacgc agcaagctct tgggatacta gagtgtttcc
     4861 caaaattgaa caaataaaca ctcttggggg aaatatgcag gttgaaggag aaatggcgca
     4921 agtagaaatc gaaccacgcg cccctggaaa actattacca ccctcaaaag aggaaaagta
     4981 aataataaaa tgctatctca aaaagccatt gatgatttca agaaattata caaagaacat
     5041 tacggcgaag agctgaatga ttttgtcgct tccgaagccg ctaatcgttt aattcgtatg
     5101 atggatttgg tatatagacc gataccgaaa gcgtgggaag aaaaatataa tgaaattaaa
     5161 aaagagcagg aagccatacc tgaaaataaa aaaacagatt ttgttaagca ccttgtaagc
     5221 gatgcgaaag tgagtgagtt cagagaaaaa attggagata aaaagttctg ggagttagta
     5281 acggaagcca atagaaaaaa taaaaaggat taactcttgc tgctgttttt taataaagct
     5341 aaatcttcat cagtcatctt gcctgctgtg gcaagactca tcaagtccat caagaatccc
     5401 attgtttctc ttttgtcggc gttagatttg aggcgcaaag cctctttgct atgtgctgac
     5461 ctggtgatgt aattgtagac gtttagcaca tagttacgtc gctttacgat tatgtcgtct
     5521 tcatagagct cctcttcatc ttggtctttc ttgagaatgt tcatagcgtc attgagcagt
     5581 tctccagcta atgtctttgc tccatcctcc aaggtttcgt ctgctggcac aactaaagca
     5641 ttttgttcgt ttcttacttt tatgagctgg cgtacttcct tctgctgttc ctggtagccc
     5701 tccaaagcca ataattcagt gtcccacttc ttgcgccata ggcgatattg tttctcccaa
     5761 gggaagaacg taagaggggc tatttgggca ttaaaatcgt ttataatctt gatggcgctc
     5821 cttcctggct ctttgctctt ctcggcgtaa tagaacgcct taaactgcgg gatgaggtct
     5881 cttatctcgt ttattttgtg gtaatgtccg tcaaagtttt cataaaaaga aaacttcttg
     5941 gtattactgg tctgtattgg tatgttatgg tctgtcatag acatattcgt tattttacgg
     6001 taatataatt atactaaatt agggttgtaa attggctgca aaactccctg ccagaatcgg
     6061 agttcttccc atatcctgtt ccaattcaat cctttcaagc gcacccgaat caaacagaaa
     6121 ttcactttta ctgattttgc ggttaacaag gggggggtgc tataataaaa acatagggga
     6181 aaccactttc actctcgctc tggcttttgc gatcggttat gcccgcgggg agttattgga
     6241 aaatatttac aaaattatca ttaaaaaacc agcagcagtt taattttcgg gcaaatttat
     6301 gctcaccaaa cttctaaaca aaaaatattt tttatttaga tttattattt tatttttagt
     6361 gtgcggtttt ggatttgcaa ataatgcggg ggcggaggtt gcttttgtcc aaagcaaagc
     6421 cgtcgatagt tggaatttat cctctttttc tgtcactttt gacagcagtg ttactgccgg
     6481 aaatatcatc gccgtaagt
//
LOCUS       DAOTEX010000047         6339 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3118
            NODE_8788_length_6339_cov_4.201941, whole genome shotgun sequence.
ACCESSION   DAOTEX010000047 DAOTEX010000000
VERSION     DAOTEX010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868142
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 6339)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6339)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 883
            CDSs (total)                      :: 833
            Genes (coding)                    :: 829
            CDSs (with protein)               :: 829
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6339
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8788_length_6339_cov_4.201941"
                     /isolate="AMR_MDS_3118"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..244)
                     /locus_tag="P5056_03335"
     CDS             complement(<1..244)
                     /locus_tag="P5056_03335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62774.1"
                     /translation="MNYLPSKKFAQKVLAISLVALGWFYVFGIKNNNSNQNSTEDSGG
                     IMTFFRTSNSSSPDMKEMGSWGDLSQEEVAAMNGKLA"
     gene            complement(484..1863)
                     /locus_tag="P5056_03340"
     CDS             complement(484..1863)
                     /locus_tag="P5056_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012174702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
                     /protein_id="HRY62775.1"
                     /translation="MAKFKKNILCIGAGYVGGPTMAVIAAKCPDYKVTVVDINKDRIK
                     AWKTDNLPIYEPGLLEIVKKARGKNLFFSTDVEKEIKENEIIFVSVNTPTKTFGEGAG
                     KAADLQYWEKTARNILKNSESDKIIIEKSTLPVRTAEAMERILNSGEKKIHFEVLSSP
                     EFLAEGTAIKDLLNPDRVLVGSRETPGGIKARNEIVEIYANWIPKEKILTTNVWSSEL
                     SKLVANAFLAQRISSINAISALCEKTEADVDEISRAIGHDSRIGAKFLRPSIGFGGSC
                     FKKDILNLSYIAESYGLHEVAEYWQNVIKLNDYQAARFVKNMLKSMFNTVAGKKIALF
                     GFAFKANTGDTRETPALAVTKMLLEERAEIIITDPKALHEAKRDLADIKKGVTLEEDV
                     YKAAKGSHAIAIMTEWDSYKTLDYEKIYKSMEKPAFIFDGRNILNHKKLYDIGFNVYP
                     IGKTPLSHL"
     gene            complement(1982..2761)
                     /gene="rsmA"
                     /locus_tag="P5056_03345"
     CDS             complement(1982..2761)
                     /gene="rsmA"
                     /locus_tag="P5056_03345"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HRY62776.1"
                     /translation="MKPKKSLGQNFLTSTAALAKIIETADLRSGDAVLEIGPGKGALT
                     EELLKTGAKVLAVEKDNELVFFLNEKFKKEVASKQLTLINGDILEINFEEKSFIKKDY
                     KIIANIPYYITGQIIRKFLTGKQKPTKMVLLVQKEVAERIVAKNGKESILSLSVKAYG
                     EPKYIIKVSRGSFFPVPKVNSAVLCIENIKEAFKNGEEKRFFEILHAGFGQKRKMILG
                     NISDIFGGKESAKKALNKCGIDPKKRSEDIDLSGWKCLSNI"
     gene            complement(2860..4827)
                     /locus_tag="P5056_03350"
     CDS             complement(2860..4827)
                     /locus_tag="P5056_03350"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRY62777.1"
                     /translation="MDNLNERQKEAAQHKDGPLLIIAGAGAGKTKTIAHRIANLIKGG
                     VEPKQILAVTFTNKAAKEMADRVRFLLPAKEEAGGSPVLCTFHSLGVRILKENAEALG
                     RSKFFSILDSDESFAFLKRAARETGFEPKDINMKGIANTVSTNKNKMIDAAEYQKEAA
                     PSFFKDAVLKIWKRYEELLKENEAYDFDDLILKTVLLLQNKKEILNYYQTLWKYIHID
                     EYQDTNTSQYEFSRLLAEKHGNICVVGDSDQSIYGWRCADFRNILNFEHNFPGAKTVL
                     LEQNYRSTGNILDLANRVIRKNKLRKEKNLFTENKTGDKITLFEAIDENDEANFVVNK
                     SRDLINKKNVRPSDIAVLYRANFQSRALEEHFIRAGIPYQLLGTRFYERKEIKDIIAF
                     IKFALNPKDNISLARIINVPPRGIGKVTLEKIISGKENEIPAGTREKINEFRRKMGGI
                     REFLLNNKLSESIKHIVKATGLDMSLKEEGKEEGMERLENIRELANITAKYNSLENEE
                     AIQKFLEDTSLMSDQDAIMDKQNREGVRLMTVHSAKGLEFEYVFVTGLEQDLFPHRGW
                     GDMEDSDIEEERRLFYVAITRAKEKLFISYAASRMIFGSRQMKMPSEFLEDIDDTIAD
                     VEKNENNFYGGSRGGGGWQKPVDDIVWDCLV"
     gene            complement(4841..5107)
                     /locus_tag="P5056_03355"
     CDS             complement(4841..5107)
                     /locus_tag="P5056_03355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62778.1"
                     /translation="MDETIEKTAEERAAEENQLRKSGDLEYLATILPRIITAKEKLEK
                     LNMYIEKYADTYMEWQNMYDKKDLEDCTKKMEDIIYRLSSVNKL"
     gene            5435..5968
                     /locus_tag="P5056_03360"
     CDS             5435..5968
                     /locus_tag="P5056_03360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62779.1"
                     /translation="MKIETIEAPKKRGRKKKEELPKTEVEAPAVPIPEEIQSVEEIVP
                     PVEAAKKPKKPRTLRDKIIENSEIAREALKEAYGKNKENNKELNDLRDTEQRLMKDLE
                     EYQLEFRELFESDSVLTNWYNRLFLKVDVEQPRDVVEFMFSKTIPKYDFLKGEILRYQ
                     RELEAVREQIKEIEKSE"
     gene            6003..>6339
                     /locus_tag="P5056_03365"
     CDS             6003..>6339
                     /locus_tag="P5056_03365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62780.1"
                     /translation="MEGFQNKIEAEIAELTRQIEAKRDILESKKGIVEEKDIVRQVVG
                     EKISSSVPAYTPKPASSLKDTGTSYLDAADEQTANIVNGLLEKVFSEGMESALKQADL
                     NDPYIIDAFH"
BASE COUNT         1660 a         1300 c         1209 g         2170 t
ORIGIN      
        1 ccgctaattt cccgttcata gcggccacct cttcttgtga aagatctccc cagctcccca
       61 tttccttcat gtcaggagaa gagctgttgc ttgtcctgaa aaaggtcata attccaccgc
      121 tatcttccgt gctattttgg ttggaattat tgttttttat gccaaaaaca taaaaccacc
      181 ccaaggccac gagggatatc gccaagactt tttgggcaaa ttttttgctt ggtagataat
      241 tcattctatt agaaaaatcc ccaaaatata ttatatcact ttatctgttg ataaagtgtg
      301 gatatatgtg gataagtttc taaagaatta ggaattacga attaagaatt aaaaatgacg
      361 ccaatctgta ttttttggat taaataaaac cctggaaatg atatatgcgt attataaaat
      421 ccgtttaaac tcaccgagtt taaacggatt cttaactcat aattcttgct tctttattct
      481 tgtttagagg tggcttaagg gggttttgcc tatcggatag acgttgaatc cgatatcata
      541 aagttttttg tggtttagaa tgtttcggcc gtcaaaaatg aacgccggtt tctccatgga
      601 tttatatatt ttttcataat ccagggtctt gtatgagtcc cattcggtca ttatcgctat
      661 tgcgtgagac ccttttgcgg ctttatatac atcttcctcg agtgtaaccc ccttttttat
      721 atcagctagg tctctttttg cctcatgtaa cgccttggga tctgtaatta tgatttcagc
      781 cctctcttcc aagagcattt ttgtgacggc gagtgcgggc gtttctcttg tatctccggt
      841 atttgcctta aaagcgaagc caaaaagggc tattttctta cccgcgaccg tattaaacat
      901 gctctttagc atatttttta caaatcttgc ggcctgataa tcgttcagtt ttataacatt
      961 ttgccaatat tccgcgacct catgtaaacc atagctttcg gcgatataac taaggtttag
     1021 aatgtctttt ttgaaacagc tacctccgaa tccgatagat ggtctaagga atttggcgcc
     1081 gattctgcta tcgtggccta tcgctctgct tatttcgtcg acatccgctt ctgttttctc
     1141 acaaagggcg cttatggcgt ttatagaaga gatcctttgc gccaaaaaag cgtttgcaac
     1201 aagcttagaa agctcgcttg accagacatt cgttgtgagt atcttttctt ttggtatcca
     1261 atttgcgtaa atctcaacta tttcattgcg tgcctttatt cccccgggtg tttctcttga
     1321 cccgaccaaa actctatccg gattaagcag gtcttttatc gccgttcctt ccgccaagaa
     1381 ttccggactg gataaaactt caaaatgaat tttcttttct cctgagttaa gaattctttc
     1441 catcgcctcc gccgtcctta caggaagagt ggatttttct atgatgatct tgtcgctttc
     1501 agagtttttc aaaatatttc tggctgtctt ctcccaatat tgtaggtcgg ctgcttttcc
     1561 cgctccctcc ccgaaagttt ttgtcggggt gtttacggac acaaaaatta tttcattctc
     1621 ttttatttct ttttctacgt cggtcgagaa aaataaattt ttaccgcgtg ccttttttac
     1681 aatctcaaga agccccggtt cgtagatagg aaggttgtct gttttccatg ccttgatcct
     1741 gtctttattt atatctacca cggtgacttt gtaatcagga cattttgccg caataaccgc
     1801 cattgtcggt cctcctacat atcccgcccc gatacagaga atattttttt tgaatttagc
     1861 cattttttgt cattcccgcg gaggcgggaa tctattttaa tttttttatg gatccccgat
     1921 tgggtcgggg atgacactgt ctactgtttt gtataatctt ttttataata ataaagtttc
     1981 attaaatatt tgaaagacac ttccacccgg acaagtcaat gtcttctgaa cgtttttttg
     2041 ggtcaatccc gcacttattt agcgcttttt tcgcggactc ttttccgcca aaaatatcag
     2101 aaatatttcc caatatcatt tttctttttt gtccgaatcc cgcatgtaaa atttcaaaaa
     2161 atcttttttc ctcgccgttt ttaaatgctt cttttatgtt ctctatacac aagactgccg
     2221 aattgacttt tggaacagga aagaatgaac cgcggcttac tttaatgata tatttcggtt
     2281 ccccataagc ttttacgctt agagaaagta tgctttcttt gccatttttt gccacgattc
     2341 tttcggcgac ttctttctga accaagagga ccatttttgt aggtttttgt tttcccgtca
     2401 ggaattttcg gatgatctgc cctgttatgt agtaaggaat atttgcgata attttgtaat
     2461 cttttttaat aaaagatttt tcttcgaaat taatttccaa aatgtcgcca tttatgagag
     2521 tcagctgttt tgaagctact tcttttttaa atttttcatt caaaaaaaag acaagctcat
     2581 tgtctttttc tacagctaaa actttcgctc ccgttttaag aagctcttcg gtgagagcac
     2641 ccttcccggg tccaatctca agcacggcat caccggaacg aagatccgct gtttcgatta
     2701 tttttgccaa ggccgcggtc gatgtcagaa aattttgccc cagtgatttt ttcggtttca
     2761 tttgattcgt tgatagttat tagtttatag tttttagaat gatttaaggt gataatgtgg
     2821 tctattttgg gctactaact aataactata aactacaaac tagactaaac aatcccagac
     2881 tatatcatcc accggctttt gccaaccgcc ccctcctctc gaaccaccat aaaaattatt
     2941 ttcatttttc tccacgtctg cgatggtgtc gtcgatatct tcgagaaact cggacggcat
     3001 tttcatttgt ctcgaaccga atatcattct ggaggcggca tatgatataa aaagtttctc
     3061 tttcgccctg gtaatggcca cgtagaacaa acgtcgttcc tcttcaatat cagagtcttc
     3121 catatctccc catcctctgt gcgggaagag gtcctgctct agtccggtga caaaaacata
     3181 ttcaaattca aggccttttg ccgaatggac cgtcatcagt ctgacaccct cccgattttg
     3241 tttatccata attgcatcct gatcggacat taaagaagtg tcttctaaga atttttgtat
     3301 ggcctcttca ttctctagag aattatattt ggcggtgatg ttcgcgagtt ctcggatgtt
     3361 ctccagcctc tccattcctt cctcttttcc ctcttctttg agagacatat ctaaccctgt
     3421 cgccttcacg atatgtttta tggactctga aagtttgttg tttagcaaaa attctcttat
     3481 cccccccatt tttcttctga actcgtttat tttttctctg gtgcctgcgg gaatctcatt
     3541 ttcttttcct gaaataattt tttccagagt gacttttcct attcctctcg gaggaacatt
     3601 tattattctg gcgagagaaa tattgtcttt cggatttagc gcaaatttaa taaaagcgat
     3661 gatatctttt atttcttttc tttcgtaaaa tctggtgccg agcaactgat aagggatgcc
     3721 cgcgcggata aaatgctctt caagggcgcg tgattgaaaa ttcgcgcgat acaaaacggc
     3781 gatatcggat ggtcgcacgt tttttttgtt gatgagatcg cgagatttgt tgaccacgaa
     3841 gttggcttca tcgttctcgt caatcgcctc aaagagagtt attttgtctc cggtcttgtt
     3901 ttccgtgaat aggttttttt ctttgcgtaa cttgtttttg cgaataactc tgttggcgag
     3961 gtctaaaata ttgccggttg agcggtagtt ttgttccaaa agaacggtct ttgctccggg
     4021 gaaattgtgt tcaaaattaa ggatatttct gaaatcagcg catctccagc cgtaaattga
     4081 ctggtcgcta tctcccacaa cacaaatgtt tccgtgtttc tccgcgagta accgtgaaaa
     4141 ttcgtattgc gaggtgttcg tgtcttgata ctcgtctata tggatatact tccagagagt
     4201 ctgataataa tttaatatct cttttttgtt ttgaaggagg agaacagttt ttagaataag
     4261 atcgtcaaag tcgtatgctt cattttcttt tagaagctcc tcatatctct tccaaatttt
     4321 caaaacagca tctttgaaaa aacttggagc ggcctctttt tgatattcag ccgcatcgat
     4381 cattttattt ttatttgtag aaacggtatt cgctatcccc ttcatattta tgtctttcgg
     4441 ttcgaagccg gtctctctgg ccgctctttt caaaaatgcg aatgattcat cgctgtctag
     4501 aatggagaaa aatttactac gcccgagcgc ttcggcattt tctttcaaaa ttcttactcc
     4561 caaagaatgg aatgtgcaga gcacaggaga acctccggcc tcttccttcg cgggcaagag
     4621 aaaacgaaca cggtcggcca tctcttttgc cgccttgttt gtgaacgtaa ccgcgagtat
     4681 ttgctttggc tccactcccc cctttatgag gtttgctatt ctgtgggcga tagtttttgt
     4741 cttccccgct cccgctcccg ctattatgag aagcggcccg tctttgtgct gggccgcctc
     4801 tttttgtctt tcgttaagat tatccattcc ggatacgttt ttatagtttg tttactgacg
     4861 acaatctata aattatatct tccatctttt ttgtgcaatc ttcaaggtct tttttatcat
     4921 acatattctg ccactccatg taagtatcgg catatttttc aatatacatg ttcagttttt
     4981 ccagtttttc tttcgccgtg attattctgg gcaaaattgt ggccaagtat tccagatcac
     5041 cgctttttct taattgattt tcctcggcgg ccctttcttc ggctgttttt tctattgttt
     5101 cgtccatccc ggttagagat aggaagcatc gcggagatta ccacttctgc ttctctatcg
     5161 attaattata ttaatttatt aaaatgcctt atatttctgc gcttccttta tttattcgaa
     5221 ccttaagtct cgatatttaa tacttacccc tatttttacg ataaaaccaa taaacaaacc
     5281 ttttttatct ctaacaggat aaatattttt ttaattattg tggtaatttc aatttgaata
     5341 atagcaggaa aagtcagact tgcgaaattg tcgtcaaaaa ccttggaaac ttgtaatata
     5401 aagtgctaat attaggaggt aataatctaa ttttatgaaa atagagacaa tcgaagcccc
     5461 aaaaaaaaga gggagaaaga aaaaagaaga gttgccgaag acagaggtag aggctccggc
     5521 tgtccccatt cctgaagaaa ttcagtctgt agaggagatt gttcctcctg ttgaagccgc
     5581 caagaagccc aaaaagccga gaaccttgag agataaaatt atagagaatt ctgagatagc
     5641 gagagaggct cttaaggagg cttatggtaa gaacaaagaa aataataaag agttaaatga
     5701 cctaagggat acagaacaga gattaatgaa agatttggaa gaataccagc tggagttcag
     5761 ggagttgttt gagagcgact cggtgctgac taactggtat aacaggctat ttttaaaagt
     5821 agatgttgaa cagccgcgag atgttgtcga gtttatgttt agcaagacaa tacctaaata
     5881 cgactttctt aagggggaaa ttttaagata tcagagagag ttggaggctg tcagagagca
     5941 gatcaaggaa attgaaaaat ctgaatagcg attttttatt aaaattgtaa caaatcggca
     6001 agatggaggg atttcaaaat aaaatagagg ctgaaatagc ggaattaacc aggcagatag
     6061 aggccaaaag ggatattttg gagtccaaga aaggcattgt tgaagaaaag gatatcgtaa
     6121 gacaggttgt cggagaaaaa attagttcat cggtgcctgc atacacacca aagcccgcta
     6181 gttcattaaa agacactggt acaagttatc tagacgccgc agacgagcag accgcaaata
     6241 ttgttaacgg tcttttggaa aaagtttttt ctgaaggaat ggaatcggcg ttgaaacaag
     6301 ccgacttgaa cgatccgtat attattgacg catttcatg
//
LOCUS       DAOTEX010000048         6333 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3118
            NODE_8799_length_6333_cov_3.625836, whole genome shotgun sequence.
ACCESSION   DAOTEX010000048 DAOTEX010000000
VERSION     DAOTEX010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868142
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 6333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 883
            CDSs (total)                      :: 833
            Genes (coding)                    :: 829
            CDSs (with protein)               :: 829
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6333
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8799_length_6333_cov_3.625836"
                     /isolate="AMR_MDS_3118"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..316)
                     /locus_tag="P5056_03370"
     CDS             complement(<1..316)
                     /locus_tag="P5056_03370"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HRY62781.1"
                     /translation="MIWATLNEMDEEVKIKPKNQHLRDMGKSDWALAADVYAKGKDVV
                     VKINLAGVDPEKIDISITADSVKVSGTAEEEEIVDNEDYFMKEIRRGSFERTIKLPVR
                     VEM"
     gene            complement(444..1148)
                     /locus_tag="P5056_03375"
     CDS             complement(444..1148)
                     /locus_tag="P5056_03375"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HRY62782.1"
                     /translation="MDNKKDLGLPIAIIISGILISASIYATGGIKIISGTAGEANLPD
                     AVPKEIVVKPADKNDHIVGKLGSDITIITYTDLECPFCQRFHATMGQIMDNYREGGKV
                     AWVYRQMPLDMLHSKSRKEAEAAECAGIVGGNVKFWEYTNKIFSYTKSNDSLDLALLP
                     QIATETGLDLKAFNTCLDSGKGKAAVINSESGAADAGAQGTPYSVILTKDGKKVPIDG
                     AMPFENIKATIDGLLR"
     gene            1648..2844
                     /gene="nusA"
                     /locus_tag="P5056_03380"
     CDS             1648..2844
                     /gene="nusA"
                     /locus_tag="P5056_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005839786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HRY62783.1"
                     /translation="MIDLKALNSALSQLEEEKGIPRDKVIEAIEMALAAAYKKDYGKK
                     NQIIRAKFDLDSGATEFVQVKTVVDESMLKPKPEVDEEGNIISPIEEEPEEEGEIKKV
                     YFNEDHHMMLADAKKIKKGVKPGEEMIFPLETKDDYGRIAAQTAKQVIIQRIREAEKS
                     SVISDFGAKEGQVVTGKVQRVEGGNVFVDLGRATGILPKDEQIPGERYRQGERIRAYL
                     YSVEDSPRGVNLKLSRSHPLFIAKLFEIEAPEVANGVVEIKSIAREAGSRSKIAVHSN
                     EANVDPVGSCVGQRGTRVSTVINELGGEKLDIVPWSENAETFITNALSPAKISSIKID
                     EEEKTAEIKVGDDQFSLAIGKGGQNVRLAAKLTGWKIDIKSSAGAKASDTDEDVEMSD
                     EDDEGI"
     gene            2934..5087
                     /locus_tag="P5056_03385"
     CDS             2934..5087
                     /locus_tag="P5056_03385"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HRY62784.1"
                     /translation="MIKNLSNNKIAISIVFSTAIILLSALISPSGSEGASAQSASNPC
                     VEESGDHRDSKPRTGGSGSSYIDKEDLRSRLTEKLKTSKLNGYVPSDGAKFGITTGSP
                     TEWANYLTELAGAESSYNNGSVGDIGRFSGNSNGLFQLSPDDALGYGLNGGKPFTLEQ
                     LRDPDINMSAAISINEAKVIKYNSIKNGAGQYWGPIQKGWLPYVPGGAGCSQNDSTAQ
                     SNAPCPPKPQTAQAQPTATQGTGEASPIQNPIFTSYWPVKGGGTQSMEGGWVTSRKGL
                     NGQNTPSTLEMVRTGESNFVTIAADPSHYGETYVIPKISYVNNQGEQHTLYNVKAYVH
                     DTGGAFRVTDADNAATRDRKLRKFDIAYDSSSGDAEAARLDGKQPSKKDVQFIPADGT
                     AVNTTSDSGAEDDPCWCKTNPQEKADCENQTQAASSITPNYGGAPLWQTSPNCFKHMG
                     NPGGPMVYYLTFTCNSGIKIDGRHTRYVADCGVNNSVGCSDWLSRLSGTAGCLWSMCK
                     GNNAIWDPGTKRCGCDDGRSLRTASVPRATVPSGSDQSLEQENPIGDTPQSIPPGEKT
                     NLSAVEEQKDSITKDNTSVLIDKSDKTMIVYKDGQEIARTNINVGVNDTAGTLAGGIN
                     GDKITPTGTFTIINTKQDSNGVYSSEGGAYMGTAFSGLSAVDQNGNIRGIGIHGGNLD
                     SGGTLHVTNGCIRVTNSDADVFSGLPVGTKVTIQN"
     gene            complement(5148..6026)
                     /locus_tag="P5056_03390"
     CDS             complement(5148..6026)
                     /locus_tag="P5056_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62785.1"
                     /translation="MKKTIYLVLILALAVPTVSFAQLLNPEKINSDIKIDTAGNISAQ
                     NVKIIQFAGNTIYGRLFWEESSIRITVKTDKASITKKYGEKMAVSEMKLDDRLNVDGS
                     LEIGTDNLTVIAKTIKNVSDQNQAGKFSGIITGPAAPYGFLLKTDSGETITLAANSSL
                     VVDKGNRRLGYNDIKVGDRVLEVEGVLNRANNILDAKRVKIYIDLSVFNGRNYQGILK
                     SLSGTELPTTATVTVGGRDYTVKLGAKTSVLNNKKYATKLSRFVIGDTVRFYGAIPEG
                     DDMYYVIGEVIRNLEL"
BASE COUNT         1894 a         1453 c         1365 g         1621 t
ORIGIN      
        1 ccatctctac tctgacgggc agtttaattg tcctctcgaa agaacctctt ctaatctcct
       61 tcataaaata gtcctcattg tctacaattt cctcctcttc ggccgttccg gaaactttaa
      121 cagaatcggc tgttatggag atatctattt tttcaggatc aactcccgcc aaatttattt
      181 ttacaacgac atccttgccc ttagcataca catccgcggc caaagcccaa tcagacttac
      241 ccatatcccg taaatgctga tttttaggct ttatcttcac ctcttcatcc atttcattta
      301 gtgttgccca gatcatattt ttaagaatta agaagaaaga attatgaata aagaatacaa
      361 catttcttct aaatatattg taacaaaaac acccgcaagg tgcgagtgtt ttctaatatt
      421 atcaactaat atctaaaaac tatttatctt aagagtccgt ctattgttgc cttgatattt
      481 tcaaacggca tagctccgtc tatcggcacc tttttgccgt ctttagtcaa aataacgctg
      541 taaggcgtgc cctgtgctcc ggcatctgcc gctccgctct cggaattaat aacagcggcc
      601 tttcctttgc cgctatcgag gcaggtgttg aacgccttta ggtcgaggcc tgtttctgtg
      661 gctatttgag gaagcaaggc tagatccaag ctgtcgtttg attttgtata agagaagatt
      721 ttgtttgtat attcccagaa tttaacattt cctccgacaa ttcccgcgca ctcggccgcc
      781 tccgcttctt ttcttgattt tgagtgaagc atatcaagag gcatctgtct gtagacccaa
      841 gcaacttttc cgccctctct gtaattgtcc attatctgac ccattgtcgc gtggaatctc
      901 tgacagaaag gacactccaa gtctgtatag gtaattattg tgatatcgga ccctagcttt
      961 ccgacgatgt gatcattttt gtccgccggt ttcaccacta tctccttggg aaccgcatca
     1021 ggcagattag cctcacctgc tgtgccgctt attattttta ttccgcccgt cgcgtatata
     1081 gaagcggaaa ttaaaatacc tgatataatt atggcaatcg gcaaacccaa atcttttttg
     1141 ttatccattt ttctgaaaat tatttagtaa ttcaccctcc taaatttttc acctttttta
     1201 attttacctc cgaataaatc agtaactaga taagatataa ctacgttgac tacttattta
     1261 atattccgaa aattttggcg ggtaaaatca atatagcaat gatatttaat tcatgcaaac
     1321 acagaaaagc ataccgccaa aacatcaaaa agaggctgat ttgatggtcc tgcctgccga
     1381 taggcgctgg tagtaatcct taccttacta tcggggcttg ccctgtagtg agctctgctc
     1441 tactacaggg ttgaccccgt agtgaatttt ggcaacggac catttaaaat ggtccacgcg
     1501 ccacagcgca ttgccaaaat ctactacggg gttgacaaga tgtctatagg gtatataatt
     1561 atggacagat tgttgttccg acccgtcgcc cgctttctag cgggcattat ttttaaataa
     1621 aattattatc atattaatta taaataaatg atagatctaa aagcattaaa ttcagccctt
     1681 tcccagctag aggaagaaaa aggcattccg cgagacaagg taattgaagc cattgagatg
     1741 gcccttgccg ccgcatacaa aaaagactac ggaaagaaaa atcagataat aagagcgaaa
     1801 ttcgaccttg attcaggcgc gacagagttc gtgcaggtaa aaaccgttgt tgacgaatca
     1861 atgttgaagc caaaaccaga agtggacgag gagggaaata taatctcccc tatcgaagag
     1921 gaaccggaag aagagggtga aatcaagaag gtatacttca acgaggatca tcacatgatg
     1981 ctcgctgatg ccaaaaaaat taaaaaaggg gtaaagccgg gcgaagaaat gatcttccct
     2041 ctcgaaacaa aagatgatta tggaagaatt gccgcgcaga cagctaaaca ggttatcatc
     2101 cagagaatca gagaggccga gaagtcttct gtaatctcag acttcggagc aaaagaagga
     2161 caggttgtta cagggaaggt tcagagagtt gagggcggaa acgtgttcgt agacctcggg
     2221 agagccacag ggatacttcc aaaggacgaa cagattcccg gcgaaagata ccgacagggc
     2281 gagaggatta gggcttatct atattctgta gaagactccc caaggggcgt aaaccttaaa
     2341 ctgagcagat ctcaccctct gtttatcgct aaactttttg aaatagaggc tcccgaggtc
     2401 gcaaacggtg tcgtagagat aaagagcatc gccagagaag ccggatcaag atcaaagatt
     2461 gccgttcact ctaacgaggc caacgttgat cctgtcggtt cttgcgtagg acaaagaggc
     2521 acaagagttt ctactgtaat aaacgagctc ggaggcgaaa aattggatat cgttccttgg
     2581 tcagaaaatg ccgaaacatt tatcacaaac gccctatccc ccgcgaagat ttctagtatc
     2641 aaaatagatg aagaagaaaa aacagcggag attaaggtgg gcgacgacca attttcactt
     2701 gcgataggca aggggggaca aaatgtaaga cttgcggcca aacttacagg ctggaaaata
     2761 gacataaaat cttcagccgg cgcaaaagca agcgatacgg acgaagacgt agagatgtcc
     2821 gacgaagacg acgagggaat ataattaaat aaacaaaaag cccgcaagcg aacagcctgc
     2881 gggctttttc attggcccat aaataatggt ataatttagt tgtaaaaata tttatgatta
     2941 aaaatctatc aaacaacaaa atcgccattt caatagtttt ttccacggcc ataattttgc
     3001 tgtccgccct catctcgcct agcggctccg aaggcgcctc ggcacaatcc gctagcaacc
     3061 cttgtgtaga agaaagtggt gaccaccgcg actccaaacc taggacaggc ggaagtggtt
     3121 ctagttacat cgacaaagag gatcttcgat ctcggctaac agaaaagctt aaaacatcta
     3181 aattaaacgg gtatgttcca tctgacgggg caaaattcgg cattaccaca ggatcaccaa
     3241 cagaatgggc taattatctc actgaattag ctggggctga atcgagctac aataatggtt
     3301 ctgtaggaga cattggaaga tttagcggga attcaaatgg actttttcag ctttctcccg
     3361 atgatgctct tggctatggg ttaaatgggg gaaaaccatt cacactagag caattaaggg
     3421 atcccgatat aaacatgagt gcggctatta gtatcaacga agctaaagta ataaaataca
     3481 actcaatcaa aaacggggct ggccaatact ggggtccgat tcaaaaaggc tggcttccat
     3541 atgttcctgg cggtgccgga tgttctcaaa acgacagcac tgcacaaagc aacgccccct
     3601 gccccccaaa accgcaaact gcgcaggcgc aaccgacggc cacacaagga acaggagaag
     3661 cctctccgat tcaaaatccg atatttacaa gctactggcc ggtaaaaggc ggaggaaccc
     3721 aatcaatgga aggaggatgg gtcacctcca gaaagggcct taacggccaa aacacgccaa
     3781 gcacgctaga aatggttaga accggagaat cgaacttcgt aacaatcgcc gcagacccat
     3841 ctcactatgg ggaaacatat gtaataccga aaatttctta tgtaaataat cagggagaac
     3901 agcacacgct atacaacgta aaggcctacg ttcacgatac aggaggagcc tttagggtca
     3961 ccgacgcaga caatgccgcc acgagagatc gaaaattaag aaaattcgat atcgcctacg
     4021 actcttcatc cggagacgca gaggcggcga ggctcgacgg caaacagcca tccaaaaaag
     4081 atgttcaatt cataccggcc gacggaaccg ctgttaatac aacgtccgac tccggcgcag
     4141 aggatgatcc gtgctggtgc aaaaccaatc cgcaagaaaa agcggattgt gaaaatcaaa
     4201 ctcaagcggc ttcttcgatt accccaaact acggaggcgc tcccctctgg cagactagcc
     4261 caaactgctt caagcacatg ggaaatccgg gcgggccaat ggtctattat ctgaccttta
     4321 cctgcaattc cgggataaaa atagacggtc gccatactag atatgttgca gactgcggcg
     4381 taaacaatag cgtcggatgt agtgactggc tttcgcgcct ctccggcacg gcagggtgtc
     4441 tgtggtctat gtgtaaaggt aataatgcga tatgggaccc ggggacgaaa cgatgcgggt
     4501 gcgatgacgg cagaagccta aggacagctt ctgtcccaag ggccacggtg ccgtctggtt
     4561 cagaccagag ccttgagcaa gaaaacccga ttggcgatac gcctcagtct attccaccag
     4621 gagagaaaac aaatttgtct gccgtcgagg aacaaaaaga cagcataacg aaagacaata
     4681 cctctgttct tatagataaa agtgacaaaa ctatgattgt atacaaagac ggacaagaaa
     4741 tagcgagaac aaatataaat gttggagtta acgataccgc gggaactcta gccggtggta
     4801 taaacggcga caaaataact ccaaccggca cgttcacgat aatcaacaca aaacaagact
     4861 caaacggagt atacagctcg gagggtggtg cttatatggg aacggctttc tccgggttgt
     4921 ctgccgtcga ccaaaatgga aatataagag gtattggtat tcacggagga aatctggact
     4981 ccggaggaac attgcacgtt acaaacggct gtatcagagt aacgaatagc gatgcggacg
     5041 tattctccgg attacctgtg gggacgaaag taactattca aaattaaatc attaacttaa
     5101 aaacgggctc gcgataagcc cgttttaatt tatacagata ttttatttta caattccaga
     5161 tttcttatca cctctcctat tacataatac atatcatctc cttccggtat cgctccataa
     5221 aatctaacgg tatcgccgat tacaaatcgg gaaagttttg tcgcatactt tttattatta
     5281 agaacagatg tctttgcgcc aagttttacc gtgtaatctc taccccccac tgtcaccgtc
     5341 gcggtggtcg gcaattccgt cccggaaaga gattttaata taccctgata gttcctaccg
     5401 ttaaagacac ttaggtctat ataaatcttc accctcttcg catccaaaat attattagcg
     5461 cggttcaaca caccctctac ctccaaaacc ctatcgccga ccttaatatc gttatatcct
     5521 agtctcctgt tgcccttatc cacaacaagg gaggagttgg ccgcaagagt aatggtctca
     5581 ccgctatctg tcttaagaag gaatccgtat ggtgcggcag gaccagtgat tattccggag
     5641 aattttcccg cttgattctg gtcggaaaca tttttaatcg ttttcgcaat caccgtgaga
     5701 ttgtctgtcc cgatttccag cgagccatcc acattcagtc tgtcgtctag tttcatttcc
     5761 gacacggcca ttttctcgcc atattttttt gtaatagacg ccttatctgt tttgacggta
     5821 attctgatag aagattcttc ccaaaacaga cggccatata tcgtattgcc ggcgaactgt
     5881 attattttca cattttgcgc gcttatatta ccggctgtgt ctatttttat gtcggaattt
     5941 attttttccg gatttaaaag ctgagcgaat gataccgtcg gcacagctag agctaatatt
     6001 aaaacaagat atattgtctt tttcattttt atttattttt acatttttct gcttacgctt
     6061 cctaatatta tacacgactt aagctatcaa caaaaaagac cccttgcaag aggccgggaa
     6121 ctgatttgat aatattccaa aagaaaagcc ccacgcattg catggggcta aatagcggcc
     6181 taggccacta gctacttgtt gtccgtcttc tgttcgggcg cgccggggtt cttgagcaga
     6241 gcctctttac gctccctctc ggccccacga agctgctggt cgacacgatg gatctcgcga
     6301 gccacaatcg gagagatggg aggcatgata ttg
//
LOCUS       DAOTEX010000049         6128 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3118
            NODE_9201_length_6128_cov_4.869751, whole genome shotgun sequence.
ACCESSION   DAOTEX010000049 DAOTEX010000000
VERSION     DAOTEX010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868142
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 6128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 883
            CDSs (total)                      :: 833
            Genes (coding)                    :: 829
            CDSs (with protein)               :: 829
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6128
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9201_length_6128_cov_4.869751"
                     /isolate="AMR_MDS_3118"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1462)
                     /locus_tag="P5056_03395"
     CDS             complement(<1..1462)
                     /locus_tag="P5056_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013252903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRY62786.1"
                     /translation="MVEKKLKKYDHNKIEKAWQKKWATAKLFKTPACRQAGRLPEKPK
                     CYALDMFPYPSGDGLHVGHPKGYIATDVYSRFKKMSGFNILHPMGWDAFGLPAENYAI
                     KNKIHPEVAVKKNIKRFKDQLQKIGFNYDWEREISTTDPAYYKWTQWIFLKLLENGLA
                     FESSEPINWCPSCQTGLANEDLDGDVCERCSSKVEKKPMRQWVLKITKYADRMLQDLD
                     KLPWPESIKELQRNWIGRSEGSEIVFAVKNSDKKIPVFTTRADTIDGVTYLVLAPEHP
                     LLQNGQLRITNYELVKKYIEEAKNRSEIERLAEKKEKTGIKLEGISVINPYNGDEVPL
                     WVADYVLGHYGTGAVMAVPQHDERDREFATKFGLPIIDRSLVPVDDVVKKVGGRKTVK
                     YKIKDWVFSRQRYWGEPIPVVHCESCGVVPVPEKDLPVTLPKVKSYAPTGTGESPLAD
                     ISKWVNTKCPKCKGPAKRETNTMPQWAGSCWYYLRYI"
     gene            complement(1634..2809)
                     /gene="glyA"
                     /locus_tag="P5056_03400"
     CDS             complement(1634..2809)
                     /gene="glyA"
                     /locus_tag="P5056_03400"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014295677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HRY62787.1"
                     /translation="MVDKEIQKLIKGEEARQKKTITLIASENFVSKDVLLALGSVLTN
                     KYSEGYPGKRYYGGQTFTDKIEVLCQKRALKAFKLKDSEWAVNVQPLSGSPANLAVYM
                     ALVPFGEKIMGMSLDHGGHLTHGHKVSATGKIWKQVPYGVNKDTEVIDYEEIKNIAIT
                     EKPKLIVAGYTAYPRAVDFKKFREIADACGAILMVDMSHFAGLVAGGAYESPFLYADV
                     VTTTTHKTLRGPRGAMIFSKRDGREFPKLIDKAVFPGLQGGPHMNQVAATAVALKEAA
                     SPEFKKYAKQVKLNAKVLAEELAKRGWKIISGGTDSHLILMDTWMGGKGIPGAEASAR
                     LEKKGIIVNKNTIPGETRSPFDPSGIRLGTAAVTTRGAKEKNMVKIAEEIDEILRSK"
     gene            complement(2926..4215)
                     /locus_tag="P5056_03405"
     CDS             complement(2926..4215)
                     /locus_tag="P5056_03405"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HRY62788.1"
                     /translation="MKVFLYDEFNPQDIAMMQALYSRSPESVTKHVEKVRQTGSGKFM
                     ERFYVGYGHASIADCGSTTLFIEGVSMLAAKAIEDWPLFSGQESSSRYIDYSKQTLGD
                     PIGTLASKKILDSWMAFYIASLPKVEEHLMAKYPRKDGEDEGVYKKAIKARAFDIMRG
                     FLPAGVHTQLSWHTNLRQAGDKLFLLKHHPLKEVRDLAEKIHAELFAKYPHSFGHKFS
                     DEQENYRKYLQSKYNYFFNADVPEFKMKTNIKTSELNKYKDIFAKRPEKTGLPNFLAE
                     LGNVTYDFLLDFGSFRDIQRHRNGVCRMPLLTTKLGFNEWYLAELPEAVRKEANGLIK
                     EQKKAIVALKTDKETAQYYTAMGFNVSCRVTYGLPAAVYVMELRSGRPVHPTLRKVAH
                     KMHAALKKAFPLMKTHPDLALTDWDVKRGLQDIRERK"
     gene            complement(4380..5039)
                     /locus_tag="P5056_03410"
     CDS             complement(4380..5039)
                     /locus_tag="P5056_03410"
                     /inference="COORDINATES: protein motif:HMM:NF013030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62789.1"
                     /translation="MEIVPAIIGKNIKEVEDKVTKVLGSVKWIQIDVMDGIFSPTKSW
                     PYTEGAISDLSKIDAIRNDDQKIEVHLMVKDPEKVIPQFVEYGADRIVFHYEATKNSS
                     EIIKFLENEEVEVAVSLMMSTPISAIDELIGEIDSVQFMSIDKIGSYGQPFDKKVFNR
                     IKDTKEKYPGLSISVDGGVSLQNAKELLDAGAEDLVIGSAIFDKSNPEEEVFKFKNIM
                     V"
     gene            complement(5155..5607)
                     /locus_tag="P5056_03415"
     CDS             complement(5155..5607)
                     /locus_tag="P5056_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HRY62790.1"
                     /translation="MAKIFISSDHAGFNLKEVLVSYLKGIGHDVLDKGPFTLDLNDDY
                     PDFISLVAAEVSKDSSSRGIILGGSGQGEAMVANRFKGVRASVFYGGTLDMIKVTREH
                     NDSNILSLGARFMTEDEAKAAVKLWLDTPFTNEERHVRRIHKIDEYPA"
     gene            complement(5631..6038)
                     /locus_tag="P5056_03420"
     CDS             complement(5631..6038)
                     /locus_tag="P5056_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62791.1"
                     /translation="MVGLTDKEISDLFPEELHKIDSLMAEQFIEPAEGVRGEGLGDME
                     RKLRVQEQIDNARKILGKERVLGPEEWRSSNAAWTKTPVAIDWTKVPEIPFSDEELET
                     AKKNGKKLALSAEGWDFRKHYTFFKALKEALLG"
BASE COUNT         1487 a         1557 c         1136 g         1948 t
ORIGIN      
        1 caatgtatct taaataatac cagcaagaac cggcccactg aggcatagtg ttcgtttctc
       61 tttttgcggg gcccttacac ttcggacatt ttgtatttac ccattttgaa atatctgcaa
      121 gtggtgattc gccggtgcct gtcggcgcat agcttttaac ttttggaagc gttacgggaa
      181 gatctttttc cggcacaggc acaaccccac atgattcaca atgaaccaca ggtatcggct
      241 ctccccagta tctctgcctt gagaaaaccc agtctttgat cttatattta acagtcttcc
      301 ttcccccaac ttttttgacg acgtcgtcta caggaacaag cgatctgtcg attataggaa
      361 gaccgaactt tgtggcgaac tctctatctc tctcgtcatg ctgtgggacg gccataacgg
      421 cgccggtgcc atagtggccc aatacataat cagccaccca aagaggcacc tcatctccat
      481 tgtaaggatt tataacgctt ataccttcta attttattcc tgttttttcc tttttctccg
      541 ccaacctttc aatctctgac ctattcttcg cctcctctat gtattttttt actaattcgt
      601 aattcgtaat tcgtaattgt ccattctgta ataatgggtg ctccggtgcg agcacaagat
      661 atgtcactcc atctatagta tctgcgcgtg ttgtaaatac tggtattttc ttgtcagaat
      721 tttttacggc gaaaacgatt tcagagccct ctgatctgcc tatccaattt ctctgtaatt
      781 cttttattga ttccggccaa ggaagcttgt ccaagtcctg caacattctg tcagcatact
      841 tagtaatttt tagcacccac tgcctcatcg gctttttctc aacctttgaa ctgcatctct
      901 cgcacacatc tccgtcgagg tcctcattcg cgaggcctgt ctgacatgat gggcaccaat
      961 ttatcggctc agaagactca aaagcaagac cattctcgag aagcttcaaa aatatccatt
     1021 gcgtccattt atagtaggcg gggtcggtgg tgcttatctc tctttcccaa tcataattaa
     1081 atcctatttt ctgaagctgg tccttaaatc tctttatgtt tttcttaacg gcgacttcgg
     1141 ggtgaatttt attcttaatc gcataatttt cggcgggcaa accaaaagcg tcccaaccca
     1201 tcgggtgaag gatattaaaa ccgctcatct tcttgaatct cgaatatacg tcagtggcga
     1261 tatacccctt aggatgaccc acatgaagtc cgtcgcctga tggatatgga aacatatcga
     1321 gcgcatagca ctttggcttt tcgggaagtc tccctgcctg ccggcaggca ggagttttaa
     1381 aaagcttagc tgtggcccat ttcttctgcc aagctttctc aattttatta tgatcgtatt
     1441 tttttaattt tttctcaacc attgttttat tattgtcttt gcgagggcga agcccgtggc
     1501 aatccagggt taactttata acattatagc tttcaatttt ataactattc tggattgcca
     1561 cacctcgatt aagacccgaa gtagtagagc agagctcact actgggcagg catctcggct
     1621 cgcaatgaca gttttattta ctccttagaa tctcatcaat ctcctcggca atttttacca
     1681 tatttttctc ttttgcgcca cgggtggtca cggcggccgt tccgagccta atgccggacg
     1741 gatcaaacgg cgaccttgtc tcgccgggta ttgtgttctt atttacaatg attcccttct
     1801 tctcaagcct tgcgctggcc tcggctccgg ggatcccttt gcctcccatc cacgtatcca
     1861 taaggataag atgactgtct gtgcccccgg aaattatctt ccatcctctt tttgccaact
     1921 cctcggccaa aaccttggcg ttcaacttaa cttgctttgc gtatttctta aattctggcg
     1981 aagcagcctc cttaagcgcc actgccgtag ccgcgacctg attcatatgt ggcccgccct
     2041 gaagtccggg gaaaactgct ttgtctataa gcttagggaa ttctcttcca tctcttttag
     2101 agaatatcat cgcgcctctc gggccgcgga gagtcttgtg ggtagtcgtt gttacaacat
     2161 ccgcgtaaag gaacgggctt tcgtaagcgc ctccggccac aagtcccgca aagtgggaca
     2221 tatccaccat aagtattgcg ccgcaagcat ctgcaatctc tctgaatttt ttgaaatcaa
     2281 cggcgcgagg gtatgcagta tagccggcaa caataagttt tggcttttct gtaatagcga
     2341 tattttttat ttcttcataa tcaataacct cagtatcttt atttactccg taaggcacct
     2401 gcttccagat ctttcctgtt gcggaaactt tgtgcccgtg tgtgagatga cctccatgat
     2461 cgagcgacat tcccattatt ttctctccaa acggcacaag cgccatatat acagcaaggt
     2521 tcgcaggcga accggaaagt ggctgaacat tcaccgccca ctcactatct ttcaacttaa
     2581 aagctttgag agcgcgcttc tgacaaagca cctctatctt gtcggtgaaa gtctgtcctc
     2641 cataatatct cttgcctggg tatccttcgg aatatttgtt tgtaagcaca gaaccaagag
     2701 ccagaagcac atcctttgaa acaaaatttt cggaagcgat aagtgttatc gtcttcttct
     2761 gtctcgcctc ctcccctttt ataagttttt gaatttcctt gtccaccatt ttaataaatt
     2821 taatttggta atgtagttgt cattgcgagc gcagcgcggc aatccagatt taaccttatc
     2881 cttatttatc ctagattgct tcgtcgtgcc tcctcgcgat gacgtctatt tcctctcgcg
     2941 aatatcttgt aaccctcttt taacgtccca atcagttagc gccagatcag ggtgtgtttt
     3001 cattaaagga aacgctttct tgagcgcagc gtgcattttg tgagcgactt ttctgagtgt
     3061 cggatgaaca ggcctgccgg aacgaagctc cataacataa actgcggcgg gaagtccgta
     3121 tgtcactcta caactgacat tgaatcccat cgcggtgtaa tactgcgccg tctctttgtc
     3181 ggtctttagc gccacgatcg cctttttctg ttccttaata agtccgttcg cctcttttct
     3241 tacagcctca ggcaattctg cgagatacca ctcgttgaag ccgagctttg ttgtaagcaa
     3301 cggcattctg cacactccat tcctgtgtct ctgaatatct ctgaacgaac caaaatctag
     3361 caaaaagtcg tatgtcacat ttccgagttc ggctaggaaa ttcggcaatc ccgtcttctc
     3421 gggcctcttc gcaaaaatat ctttatattt gtttaattcg gaagttttta tattcgtctt
     3481 catcttaaat tccggaacat ctgcgttgaa gaagtagttg tatttgctct gcaaatattt
     3541 tctgtaattc tcttgctcgt cggaaaactt gtgtccgaag ctatgaggat atttagcaaa
     3601 aagttcggcg tgtatctttt cggcaagatc tcgcacctcc ttaagcggat gatgcttgag
     3661 caaaaagagc ttgtcccccg cttgtctcaa atttgtatgc catgaaagct gagtatgaac
     3721 tccggccggc aagaatcctc tcattatatc gaacgccctc gccttgatag ccttcttata
     3781 aaccccctca tcttcgccat cctttcttgg atattttgcc attaggtgtt cttcaacttt
     3841 cggcaaagag gctatgtaaa aagccatcca actatccaag atcttttttg aggctagggt
     3901 tccgatcgga tctccaagtg tctgcttgga gtaatcaata tatcttgagc tagattcttg
     3961 tcccgagaaa agcggccagt cctctatcgc tttcgccgca agcatactca ccccctctat
     4021 aaacagcgta gtgcttccac aatcagcgat gctggcgtgt ccatagccga cataaaatcg
     4081 ctccatgaat ttaccggaac cggtctgcct cactttctcg acatgcttcg tcacactttc
     4141 cgggctcctt gaatagagcg cctgcatcat cgctatgtct tgaggattaa attcgtcgta
     4201 taaaaaaact ttcatctttc ttttaactaa aaaaataata aaaacacttt gtcattgcga
     4261 gccgaagcgt cttagctgag gcgtggcaat ccatgtaact atatcgagat aagtctggat
     4321 tgcttcgcta cgctcgcaat gacgacgatt tttgacttta tgggcttaat aactatctct
     4381 tacaccataa tatttttaaa tttaaaaacc tcctcttcgg ggttgctctt atcaaatatt
     4441 gcagaaccta tgacgaggtc ctctgcaccc gcatccaaca attcttttgc gttttgaaga
     4501 cttactcctc cgtccacaga aatagataat ccgggatatt tttcttttgt atcttttatc
     4561 ctattgaaaa cttttttatc aaacggctgc ccgtaactgc cgatcttgtc gatgctcata
     4621 aactgaacgc tgtctatttc gccgataagc tcatctatag cagatatcgg cgtgctcatc
     4681 atgagtgata ctgccacctc tacctcttcg ttttctaaaa acttaattat ctcagaacta
     4741 ttttttgtag cttcataatg aaaaacaatc ctatctgcgc catattcaac aaactgcgga
     4801 ataacttttt cgggatcctt aaccataaga tgcacctcta tcttttggtc gtcgttcctt
     4861 atcgcgtcta tcttggatag gtcgctaatt gcgccttctg tataaggcca gctttttgta
     4921 ggtgaaaaaa taccgtccat tacatctatc tggatccatt tcacagaacc aaggactttt
     4981 gtgaccttgt cctcgacctc ttttatattt ttaccgatta ttgcaggtac aatttccatg
     5041 tgtttagttt atagttttta gttgatagtt aatagtaata gttttttcta acaactaatt
     5101 actaccaact acaaactctt caaaaaatcg ccgtaaacgc gaccaacaaa cgaactacgc
     5161 cggatactca tctatcttat gtatacggcg gacgtgtctt tcttcattgg tgaaaggcgt
     5221 atcaagccaa agttttaccg ccgcttttgc ctcatcttct gtcataaatc ttgcgcccag
     5281 cgaaaggata tttgaatcat tgtgctctct ggtgactttt atcatatcga gtgttccacc
     5341 ataaaaaaca gatgccctga cccccttgaa cctatttgcg accatcgctt caccctgacc
     5401 ggaaccacca agaattatcc ctcttgaaga tgaatcttta gaaacctccg cagcaactaa
     5461 ggaaataaaa tcaggataat cgtcattaag gtcgagtgtg aaaggcccct tatcgagcac
     5521 gtcatgacca atccctttta gatagctcac aagaacctcc tttagattaa atcccgcatg
     5581 atcggatgaa ataaaaatct tggccatatt ttaaaaagtt aacagcagcc ttatcctaat
     5641 aatgcctcct tcagtgcctt aaaaaatgtg tagtgttttc ggaagtccca cccctctgcg
     5701 ctgagcgcta gcttcttccc atttttcttc gcagtttcaa gttcttcatc ggaaaaaggt
     5761 atttctggaa cttttgtcca atcaatcgca actggtgttt ttgtccaggc ggcattagac
     5821 gacctccact cctccggccc caaaactcgc tctttcccaa gtatcttcct cgcattatca
     5881 atctgttcct ggaccctgag ttttctctcc atatcgccaa gcccctcacc acgcacgccc
     5941 tcagctggct ctatgaattg ttcggccata agactatcaa ttttatgcag ctcttcgggg
     6001 aagagatcgc taatttcttt atcagtaagc ccaaccatct tgcgggcgat ttctctggcc
     6061 tcttcttttt tcttctcctc ttcttctcct ttcttttcta tatctttgtc gacaacctac
     6121 tctatttc
//
LOCUS       DAOTEX010000050         5590 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3118
            NODE_10387_length_5590_cov_4.656730, whole genome shotgun sequence.
ACCESSION   DAOTEX010000050 DAOTEX010000000
VERSION     DAOTEX010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868142
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5590)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5590)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 883
            CDSs (total)                      :: 833
            Genes (coding)                    :: 829
            CDSs (with protein)               :: 829
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5590
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10387_length_5590_cov_4.656730"
                     /isolate="AMR_MDS_3118"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /locus_tag="P5056_03425"
     CDS             complement(<1..340)
                     /locus_tag="P5056_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013537158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HRY62792.1"
                     /translation="MKLLIVESPAKAKTISKYLDNEYTVRASVGHIRDLPKSNKEAID
                     IEGGFIPKYEISKGKEKVVSELKELSKKSSEILLATDPDREGEAIAWHVAEALKLKNP
                     KRVTFHEITER"
     gene            complement(363..632)
                     /locus_tag="P5056_03430"
     CDS             complement(363..632)
                     /locus_tag="P5056_03430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009184717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HRY62793.1"
                     /translation="MFYIYLLQSRIDEHLYIGYTTDLKKRVDEHNRGLNLSTKRYGKW
                     NLIYYEACLNERDAKRREKYLKTTQGRRMLKRRLKDYFYEGRGKI"
     gene            920..1153
                     /locus_tag="P5056_03435"
     CDS             920..1153
                     /locus_tag="P5056_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62794.1"
                     /translation="MNGVSKPPPSSPDNSKPKYSPTFKRMTMPRQITAVIVPENTRHQ
                     IVGNIGKSAIVNIAKNKRAKRPNPISTTLLLLP"
     gene            complement(1280..1549)
                     /locus_tag="P5056_03440"
     CDS             complement(1280..1549)
                     /locus_tag="P5056_03440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62795.1"
                     /translation="MLISAKKPFSKNFKKEVEIMLLWILYSLLVLAGIFVFPELTLAV
                     VLWNSGYAILGVVALFAIYVPGFKEKGVRIIIGLWYDFVNLFRKE"
     gene            1692..2384
                     /locus_tag="P5056_03445"
     CDS             1692..2384
                     /locus_tag="P5056_03445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62796.1"
                     /translation="MKKFLILVAIFALVFSFSLPAFSEAKYKDLTLDQIEKLVDRLGG
                     MDGVKNVMSDSVAVILVLDADDNKNGTDVHMIVVQKEVPPKRKLLPSTAIGPTEVKLT
                     KDLLTSALWARGYKSSPRINGVFVKYFPPCGTIIKCWIVSYHDNPTFAEVIADMNEGG
                     YRPASLLEGAAFMIENPKWVSDGTGMPILLLGEMYKGSYPALCRQDGANTVDLFLDSA
                     ETKKVFYLAIEK"
     gene            2459..3373
                     /gene="murB"
                     /locus_tag="P5056_03450"
     CDS             2459..3373
                     /gene="murB"
                     /locus_tag="P5056_03450"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:TIGR00179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HRY62797.1"
                     /translation="MINVQKNVSIKPHLNFEIGGSVSHFAEVATPEEIIYAIKHAKSL
                     KLPHIILGGGNNVVFCDKKKKCFLIKIAGGDVTIQGEHAYAGAGVDLFEFILKMIKAG
                     LGGLETLSGIPGTIGGAIYGNAGAYGHSISECVESVTIFDGKKIRKVSNKDCKFEYRE
                     SIFKKKKWIILGADFKFQKADPLALQKISDDITKVRWEKFGKKPRCPGSYFKNVLVKD
                     ISKKSLKLIDPAKIRDGKIPAGYLLEEVGAKGMHEGGVYVSEFHGNIIMNDGTGSYKD
                     LMKLVHKLRMLVKKKFGIELEEEVRYFV"
     gene            3783..4058
                     /locus_tag="P5056_03455"
     CDS             3783..4058
                     /locus_tag="P5056_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62798.1"
                     /translation="MVIILELVACCLCIVGAHYGARRNAQIGNYFRELVRKTQSAHVD
                     DIKNGRDSSWRWKALNKFPYIWFLMLLCFWRPLDSFVTDKSFAEIQK"
     gene            complement(4071..4340)
                     /locus_tag="P5056_03460"
     CDS             complement(4071..4340)
                     /locus_tag="P5056_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62799.1"
                     /translation="MCPIWIRKSPELTGLHEASTFLILLGANAEAGELRAATAIAFTL
                     ALMFVLALFRRRIVGLMLENGDFEVIQEQAHEDSHEGVKQNPHNH"
     gene            4405..>5590
                     /gene="gatB"
                     /locus_tag="P5056_03465"
     CDS             4405..>5590
                     /gene="gatB"
                     /locus_tag="P5056_03465"
                     /inference="COORDINATES: protein motif:HMM:NF004012.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HRY62800.1"
                     /translation="MSAIKYYPTIGLEIHAELRTKTKMFCNSKNDPDEKRPNVNICPV
                     CMAHPGTLPVINKEAVKHVLRVGHAIGGNIADFTEFDRKNYFYPDIPKGYQISQYKYP
                     LVSGGFINGILVTRIHLEEDTAKSYHRENDSNSYIDFNRAGVPLMELVTEPVIHSAKE
                     AGDFARELQTLLRYLGASEANMEKGEMRVEANISVSADPDKFGTKVEVKNLNSFKAVE
                     RAIDFELARHIKLLDEGKGDEIIQETRGWDEFKQVTFSQRKKESSHDYRYFPDPDLPK
                     LWISKIKEFGELKNSMPELPREKRERFLNEYGLRNEAVEIFINERALADYFESAVSGK
                     KDDKAFCALVSNYIVGDLLSLMKKAGVSDTDELNEVNKDSIRVKSEDLAALVSMIVAG
                     ELS"
BASE COUNT         1617 a         1039 c         1340 g         1594 t
ORIGIN      
        1 cacgctccgt gatctcgtgg aatgtgacac gcttggggtt tttaagcttg agtgcctcgg
       61 cgacgtgcca ggcgatagct tctccttctc ggtcggggtc cgttgcgagc agtatctcgc
      121 tagatttctt tgagagttct ttgagttctg aaacaacctt ctcctttcct tttgaaattt
      181 cgtatttagg gataaagccg ccctcgatgt cgatggcttc tttgttactc ttcggcaggt
      241 cgcgtatgtg cccgacgctc gctctgaccg tgtattcatt atccaaatat ttggaaatgg
      301 tcttcgcttt tgccggtgac tctacaatta aaagtttcat cccgtagtag aattttagtt
      361 aattaaatct ttccccgacc ttcataaaag tagtctttta atcttctttt taacattcgt
      421 ctgccctgcg tggtcttgag atacttttct cttcttttgg catctctctc gttaagacat
      481 gcttcgtagt atatcagatt ccatttgccg tagcgcttgg tggaaagatt taatccacga
      541 ttatgttcgt ccactctttt cttcaggtcc gttgtgtatc ctatatataa atgttcgtct
      601 atgcggcttt gcagtaaata aatatagaac ataatcaaac taaaatttca ctacgggatt
      661 cattctattt aagttgtttt gagtatagta gtcttattat ataggaaatg tcaacccagt
      721 agtagaattt ggcaacggcc aaagccgcgc agagcgcatt gccaaatttc actactgggt
      781 caaataaagc gcatcctgtg gaaaagtatt tatagtattg ttttgggggc caaaaaccaa
      841 tagaaaaagc acccgggtgg gtgctttgct tcattgtttg gggtagctga gatgtttttg
      901 ttttgcgctg gtttaaggaa tgaatggggt atcaaaaccg cccccatctt cgccagataa
      961 ttcaaaacca aaatattccc cgaccttcaa gagaatgaca atgcccagac aaatcactgc
     1021 cgtaatcgtg cccgaaaata ccagacacca aattgttgga aatattggca agagcgcaat
     1081 tgtgaatatt gcaaagaata aaagagcaaa acgacccaac ccaatctcca caacgctcct
     1141 ccttttgccg tagagcccta gggcgttttt cgttttaaga accaaccggc attatttata
     1201 cattaattta attttttggt caaattagga aaaattgtcg tttagagaaa aagcacccgg
     1261 gtgggtgctt acatataggt tattctttgc gaaacaagtt gacgaagtca taccacaggc
     1321 caatgatgat tctgacgccc ttttctttaa atccgggcac atagatcgcg aatagtgcaa
     1381 cgacaccgag gatagcgtat ccgctattcc agagaacgac tgccagcgtc agttccggga
     1441 acacgaagat gccggccaga actaagagcg agtagaggat ccataggagc atgatttcaa
     1501 cctccttttt gaaatttttg gagaacggtt tcttcgctga tattagcact attttctttt
     1561 atatcaaggc tggtttacat ttacataaaa ataagtagta tgagcggaga aaacaaatat
     1621 atgaaacagg gttcttttac aacaacaatt caaatttaat tccacgaaag tggaggaagg
     1681 gaaggtcttg tatgaagaag ttcttgattc tcgttgcaat ctttgcgctc gtattcagtt
     1741 tttctttgcc tgcgttttct gaggcaaagt acaaagactt aactctcgat cagattgaga
     1801 agctcgttga taggcttggt ggcatggatg gtgttaagaa tgtgatgtcc gatagcgtcg
     1861 cggtaatctt ggtcctggac gctgacgata ataaaaacgg cactgacgtg cacatgatcg
     1921 ttgtgcagaa agaagtaccg ccgaaacgga aacttctgcc ttccactgcc attgggccca
     1981 ccgaagtgaa gctgacgaaa gatcttttga caagtgcgtt gtgggcaaga ggctataagt
     2041 cgagcccccg gattaatggt gttttcgtta aatattttcc tccgtgcggt acgataatca
     2101 aatgctggat tgtctcttat catgataacc caacatttgc tgaagttatc gcagatatga
     2161 atgagggtgg gtatcgcccc gcgtcgcttc ttgagggagc agccttcatg attgaaaacc
     2221 cgaagtgggt tagcgacggt accggcatgc ccattctatt actcggcgaa atgtacaagg
     2281 gttcttatcc ggcgctctgt cggcaggacg gcgccaacac agttgacctt ttcttagaca
     2341 gcgcggaaac gaagaaagta ttttatttag caatcgaaaa ataaatttga accgcagtct
     2401 gtgtcgtccg ctccttcggg ggcggatttt ctttttcaaa atatgataaa atctgcctat
     2461 gataaatgtc caaaaaaatg tttcgataaa gccgcatctt aattttgaga taggtggtag
     2521 tgtctctcat tttgcggaag tggctactcc agaagagata atttacgcga taaagcacgc
     2581 gaagagtttg aagctccctc atattatatt aggcggaggg aacaacgttg ttttttgcga
     2641 caaaaagaaa aagtgttttt tgataaagat agcgggcggc gacgtcacga tacaaggtga
     2701 acatgcatac gcgggcgcag gtgtagatct gtttgaattt attttaaaaa tgataaaggc
     2761 gggactaggc gggcttgaga ctttgtcggg cattcccggg acgataggtg gcgcgattta
     2821 cggtaatgca ggtgcatacg ggcactctat ttctgagtgc gtggaatcgg ttaccatatt
     2881 tgatggtaag aaaataagaa aggtgtcgaa taaagattgt aagtttgaat atcgcgagag
     2941 catttttaag aaaaagaaat ggataatact tggtgcagat tttaaattcc aaaaggctga
     3001 tcctctggcg ctccaaaaaa tatccgacga tattacaaaa gtgaggtggg agaaatttgg
     3061 taagaagcct aggtgcccgg gaagctattt caagaacgtt cttgtaaaag acatttcaaa
     3121 aaagtcgcta aaacttattg accctgcaaa aataagagac gggaagatcc cggccggata
     3181 tttgctcgaa gaagtcggcg cgaaaggaat gcatgaaggc ggggtatatg tatcagaatt
     3241 tcacggaaat ataataatga atgacggcac cgggagttat aaagacctca tgaagttggt
     3301 ccataaatta cgaatgcttg tgaaaaagaa gtttggaata gaactcgagg aggaggtgag
     3361 atacttcgta tagccccagt caaataggag gtatttatga ctatttgtta caaggtgaaa
     3421 tttgagatgg ccgatattcg ttggcggtca agagttaatt tgacggggta aaaggataga
     3481 taatagaaga tagaaagtag aaagtagtta aaaaagagaa tattttatat acacaaataa
     3541 agtagcgacc gctactttat ttgtgtatgg agtggtgcgg tgttttcaga ccacttaaca
     3601 atcacattat tgtttttggg tgtgtatcaa ataacttagc ggtcgctaag ttatttgata
     3661 caggtgtttc cgggttattg aatcatgacg ctgttttgac atgtgaataa atttttggta
     3721 gaagtagttt ggttctggct cgttgaaaat aaaaaataat caatccgaaa ggggagactg
     3781 cggtggttat catactagaa ctcgtcgcat gctgtttgtg cattgtcggt gctcattatg
     3841 gagcgcgaag aaatgcgcaa attggcaatt acttcagaga attggttcga aagactcagt
     3901 cggcacatgt tgatgatatc aagaatggcc gcgacagttc ttggagatgg aaggcactca
     3961 ataagtttcc gtatatctgg tttttgatgc ttctttgttt ttggcggccc ctcgatagct
     4021 ttgtgaccga caaatcgttc gctgagatcc agaaataatc tgaaaggagg tcaatggtta
     4081 tgtggatttt gctttacgcc ttcatggcta tcctcgtgtg cttgttcctg tattacctcg
     4141 aaatctccat tttccagcat gagcccgaca atcctccgcc tgaagagtgc gagaacgaac
     4201 atgagtgcga gtgtgaaggc gattgccgtt gcggcacgga gttcaccggc ctctgcattt
     4261 gccccaagaa ggattagaaa agttgaagct tcatgaagcc ccgttaactc ggggcttttt
     4321 cttatccaaa tcggacacaa ctagttgttt gtgacaagtt tttgtatgaa ataaatttat
     4381 agtatactta cttagacaaa taaaatgtcc gcaataaaat attacccaac tattggtctt
     4441 gagatacacg ccgagcttcg cacaaagacg aagatgtttt gtaattcaaa gaatgatccg
     4501 gatgaaaaaa gaccgaatgt aaatatttgc cctgtatgta tggcgcatcc gggaacattg
     4561 ccggtcataa acaaagaggc ggttaagcac gttcttcgag tcgggcacgc aataggcggc
     4621 aatatcgccg atttcacaga gtttgataga aaaaattatt tttatcccga tattccgaag
     4681 ggttatcaaa tatcacaata taaatatccg cttgtctcgg gcggttttat aaatggaatt
     4741 ttggttacaa gaatacatct ggaagaagat accgctaagt cttaccacag agagaacgat
     4801 tctaactcat atatagattt caatcgtgcc ggagttccgc tcatggagct tgtgactgag
     4861 ccggtaatac acagcgccaa agaggcgggt gattttgctc gcgagcttca gacacttcta
     4921 agatatttgg gtgcatcgga ggcgaatatg gaaaaggggg agatgcgagt tgaggcgaac
     4981 atttctgttt cggctgatcc cgataagttt gggacaaaag ttgaggttaa gaatttaaat
     5041 tcgttcaagg ccgttgaaag agcgattgat tttgaacttg caagacatat taaactccta
     5101 gatgagggta agggcgatga gataattcaa gagacccgcg gatgggacga gtttaagcag
     5161 gtcacgttct cacagagaaa gaaagagagt tcacacgatt atagatattt tcccgatccg
     5221 gacttgccga aactttggat aagcaagatt aaggaatttg gagaactcaa gaacagtatg
     5281 cccgagcttc caagggagaa gagagaaaga tttttgaacg aatacggcct taggaatgag
     5341 gcggtggaga tatttataaa cgaaagagcc ctggccgact attttgaaag tgcagtttcg
     5401 ggcaagaaag acgacaaggc attctgtgcg cttgtttcta attatattgt aggagatctc
     5461 ctgtcgctta tgaaaaaggc gggcgtttcc gatactgacg aattgaacga ggttaataaa
     5521 gattcgattc gcgtaaagtc ggaagatctc gccgcgcttg tttctatgat tgtggcggga
     5581 gagttatctt
//
LOCUS       DAOTEX010000051         5327 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3118
            NODE_11147_length_5327_cov_4.168437, whole genome shotgun sequence.
ACCESSION   DAOTEX010000051 DAOTEX010000000
VERSION     DAOTEX010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868142
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5327)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5327)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 883
            CDSs (total)                      :: 833
            Genes (coding)                    :: 829
            CDSs (with protein)               :: 829
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5327
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11147_length_5327_cov_4.168437"
                     /isolate="AMR_MDS_3118"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..795)
                     /locus_tag="P5056_03470"
     CDS             complement(<1..795)
                     /locus_tag="P5056_03470"
                     /inference="COORDINATES: protein motif:HMM:NF012796.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase"
                     /protein_id="HRY62801.1"
                     /translation="MRQSKLFTKTRREAPTDETAKNAQLLIRAGYLHKEMAGVYSYLP
                     LGLMVIKKIEGIIREEMSAIGGTEMKTSVLQNKEVWEKSNRWDDNVVDNWFKTKLKNG
                     GEVGLSFTNEEAYSNIIKQHISSYKDLPVYPYDFKEIFRNEARSKSGIMRGREFYWKA
                     LYSFSKDEAEHNDFYERAKSAYKNIFERVGIGDKTYLTFASGGTFSKFSHEFQTITDA
                     GEDTIYIDEEKKIAVNKEVYEDEVIKGLGLDKNKLVEKKTVEVGNIF"
     gene            complement(1133..2248)
                     /gene="rseP"
                     /locus_tag="P5056_03475"
     CDS             complement(1133..2248)
                     /gene="rseP"
                     /locus_tag="P5056_03475"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HRY62802.1"
                     /translation="MSLILFFIVLAILILSHELGHFAVAKYFGVRVDEFGFGFPPRLF
                     VKKRGETEYSLNLIPFGGFVKIFGEDETDEYLKTPEKDRSRSLLNQPKKVQAAVILAG
                     VFCNFLLAWFLISTTITLGAKVSATSLPERLKASEVGLVVMNVVPGSPADKAGLKSGD
                     EVVYVASIERTLQEGSLAVKNVQDLIASSKDKDIVIGFSRDRAPAQSIIVTPVSGIVE
                     DRGAIGVSLDMVGTLKLPFYRSIYEGFIITIRLVAEMVVGLVDFVYGFFVGSSSVGQV
                     SGPVGMVGIVGEAGRRGIVELLNMTALISINLAVINLVPFPALDGGRLLFLVVESLRG
                     KPINKKATATMNAIGFLLLLSLMFAVTYSDISKIFFK"
     assembly_gap    2574..2583
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2584..2978)
                     /locus_tag="P5056_03480"
     CDS             complement(<2584..2978)
                     /locus_tag="P5056_03480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-recycling factor"
                     /protein_id="HRY62803.1"
                     /translation="MAYNFSAFKEKTASTANWLVQEFSGLRTGRATPALLDNVAVDSY
                     GVKVPITHIASISTEDARSLKIVPWDKSSIKDIERAVAAANLGVSTSPDANGVRVIFP
                     ELTEDRRKALAKIIKEKLEDARVTIRKER"
     gene            complement(3058..3789)
                     /locus_tag="P5056_03485"
     CDS             complement(3058..3789)
                     /locus_tag="P5056_03485"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62804.1"
                     /translation="MNELEKILEDIRIGGQHAFLITAETKNVLPLLRDGIKEVFKKKI
                     ENDPDYYEFVTEKLNVEGARRIRMASQRRSMGGGNRFFIIGADSLLEEAQNALLKTLE
                     EPIAGHFFFIITPNEHLLLPTVRSRMQKLNFSPEAVIGDSLSEKFIESSVQDRLLMIP
                     EIMKEDAEPSTQRINVKNFIVNLKKKVFDEYKGRDFYDKKNFEQVMSEITKAEKYSGD
                     VASAPRLLLEFLSFICPRKQVGDLE"
     gene            4017..4679
                     /locus_tag="P5056_03490"
     CDS             4017..4679
                     /locus_tag="P5056_03490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62805.1"
                     /translation="MTIFYVVAASLVGLLITFCKFRGVTIGNIIARSEAVLEEYADDN
                     ENKIHEHALIVRNPNDKSRDARAIIVSESDLAELLLKKIFIGLSLLFPAFTQLFYFCG
                     GTSLGVTGSVFMSLIMSTMVSIFSVAILNKFSRFHPLCFREESLSYIAFGPKFSKGVS
                     TILLDAAEYELKKYDEEEAEEKAIAEMPENTAEEKKAKAIAEVKLKIKKIPFPKNRTI
                     FG"
     gene            complement(4766..>5327)
                     /locus_tag="P5056_03495"
     CDS             complement(4766..>5327)
                     /locus_tag="P5056_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HRY62806.1"
                     /translation="MVVDIGGGTTDCAVISLGGIVYANSVKCAGNKVDQAIADYIKKT
                     FNLAIGDKTAEEIKIKVGSAIPVDEEQKMMIKGRDFLSGLPRTADITTNEIAKAIANE
                     MKQVVKAIKDVLQETPPELSADIIDQGIIMTGGTSMLANLPELIYRKTGVKAILADDA
                     LYCVAKGTGIALNHLDAYKQSIMSKR"
BASE COUNT         1528 a         1263 c          944 g         1582 t
ORIGIN      
        1 gaaaatattt ccaacctcca cagtcttctt ctcaacaagt ttatttttat ctaatcccaa
       61 tccctttata acctcatctt cataaacttc tttgttgact gcgatcttct tttcttcatc
      121 tatatatata gtgtcttcgc cggcatcggt tatcgtctga aactcgtgag agaattttga
      181 aaacgttccg cccgacgcga atgtcaggta tgtcttgtct cctatgccca ctcgttcaaa
      241 tatgtttttg taagcggact tggctctctc gtaaaaatca ttatgctccg cttcatcctt
      301 tgaaaatgaa tacaaagcct tccagtaaaa ttctctgccc ctcataatcc ccgacttact
      361 tctcgcttcg tttctaaaaa tttctttgaa gtcgtaaggg taaacaggaa gatctttgta
      421 agagcttata tgctgtttga taatatttga ataagcctct tcgttagtga aagacaatcc
      481 aacctctccg ccattcttta attttgtttt gaaccagtta tcgactacat tatcatccca
      541 tctattagac ttctcccaaa cctccttgtt ctgaagcacg gaagttttca tttcggtccc
      601 accaatagcg ctcatctctt ctcttataat accctcgatc tttttgataa ccataaggcc
      661 gagcggaaga tatgaataaa ctccggccat ttctttgtgc agataacctg ctcttatcaa
      721 aagttgtgcg ttctttgcgg tctcgtcagt aggtgcctcc cttctcgtct ttgtaaataa
      781 tttagattgt ctcataagcg caatattagc ataaaaaaac cgcccctgtg aaggagcggg
      841 aatatcaatt tattaaccgg cgaaggcgat tggtcccacg ataaaaaata aactcaaata
      901 aaaaattaat aaggagctgg tcaagaaaag caggatcacc atagagaggc tcatttctac
      961 cggactgttc gcatcattaa taaaagcgac tatgtaggta aaagcaaacc cgagaaacga
     1021 gctcacaacc aagaggaata agttcatgac aagccacctc ctttttctga gatattaaag
     1081 atcgtatcta aggaagatac tacacccaaa aaagtcgatt gtaaaactat ttttatttaa
     1141 agaaaatctt ggaaatatcg ctgtaagtaa ccgcaaacat aagggaaaga aggagtaaga
     1201 agccgattgc attcatcgtg gcggtcgctt ttttgtttat cggcttcccg cgcaaagatt
     1261 caacaacgag aaaaaggagt ctccctccat caagcgcagg aaacggcaca agatttatca
     1321 cggcaagatt tattgatatg agcgcggtca tatttaacaa ttccacaatc ccgcgcctgc
     1381 ccgcctctcc cactatccca accatgccca caggaccgct aacttgccct accgaagaac
     1441 tgccgacaaa aaatccatac acgaagtcaa cgaggccaac aaccatctcg gctaccaaac
     1501 gtattgtgat tataaaccct tcataaattg atctgtaaaa gggaagtttt agggtgccca
     1561 ccatgtcgag cgacacaccg attgcgcccc tatcttccac aatgccggat accggcgtca
     1621 cgatgatgga ttgcgcagga gccctatccc gcgaaaaacc aatcacgata tctttgtctt
     1681 tgctcgatgc tatcaagtcc tgaacatttt ttaccgcgag agatccttcc tgaagagttc
     1741 tctcaattga tgcgacataa acaacctcgt ctccggactt caatccggcc ttatctgcgg
     1801 gggaacccgg cacgacgttc attactacaa gcccgacttc gctagccttt aatctttcgg
     1861 gtaaagacgt ggcggaaact tttgctccca gagttattgt cgtcgatatc aaaaaccaag
     1921 ccaacaaaaa gttacaaaaa acaccggcca gaataaccgc ggcctgaact ttctttggct
     1981 gatttaataa agatcgagac ctgtctttct ctggcgtctt aaggtattca tctgtctcat
     2041 cctctccaaa tattttcaca aacccgccga acgggatcaa atttaaacta tattctgttt
     2101 cccctctttt ctttacaaaa aggcgcggag gaaaaccgaa gccgaactca tcaaccctta
     2161 cgccaaaata ttttgcaacg gcaaaatgcc caagctcatg agacaggatt aaaatcgcca
     2221 aaactataaa aaataatatt aagctcatta agataagaat taggaattat gaataaagaa
     2281 ataagaaata ttttatataa acttttagtt gaacggctta gcaaataaat tttaaaacaa
     2341 aagcggaatt ataaataaga gaaagatgat gaaaaaattt tatttttctt cctaattcgt
     2401 aattcttgat tcctgcttct tttttaagac attatttcct tttccttctt ctcggaaagg
     2461 tcctcaagat ttttgtttgc ggcatcaaca agcttttgca attcatcctt caaggtaaat
     2521 ttatcatcat cggtcaaaac tccatctttt actttcgtct gaatatccgt ccannnnnnn
     2581 nnntctctct cttttcgaat agtcactctg gcgtcttcca acttttcttt aattatctta
     2641 gccagggcct ttcttctatc ctcagtaagc tcggggaaaa taacgcgaac gccattcgcg
     2701 tcaggagatg tagaaacacc gaggttggcg gcggccaccg ctctctctat atccttaata
     2761 ctgctcttgt cccaaggaac gattttcaga cttctagcat cttctgtgct gatgctcgcg
     2821 atatgcgtta tcgggacctt aaccccataa gaatcaaccg ccacattatc taagagcgca
     2881 ggagtagctc ttcctgtcct taaaccggaa aattcttgca caagccaatt ggctgtggag
     2941 gctgtttttt ctttaaacgc ggaaaaattg tatgccataa ataattaaat tatcaattac
     3001 taattacgaa ttaacaatta aaatatatca caaatctgga actgatgtaa ctacccgcta
     3061 ctctaaatca cccacctgct tcctcggaca aataaaagac aaaaattcca agagaagtct
     3121 gggtgccgat gcgacgtctc ctgaatattt ttcggctttc gttatttcgg acattacttg
     3181 ttcaaaattt ttcttgtcat aaaaatcacg ccccttatat tcatcaaaaa ctttcttctt
     3241 caaattaacg ataaaattct tcacgttaat tctctgggtt gagggttcgg catcttcttt
     3301 cattatttcc ggaatcatca aaagtctgtc ctggaccgaa gactcgataa atttctcgct
     3361 caaagaatct ccaataacgg cctccggaga aaaattcagt ttttgcatcc tagaccttac
     3421 cgtgggcaaa agcaggtgct cgttgggggt aattataaag aaaaagtgtc ccgcaatcgg
     3481 ttcttcaagg gttttcaaaa gagcgttctg tgcctcttcc aaaagactat cggcccctat
     3541 aataaaaaac ctgttccctc cgcccatact tcttctctgg cttgccatcc ttatccttct
     3601 ggccccctcg acgttcaatt tttctgtaac aaattcgtaa taatcgggat cgttctctat
     3661 tttcttctta aaaacctcct tgataccgtc tcgtagcaga ggcaaaacat tctttgtttc
     3721 tgccgttatc aaaaaagcat gctgaccgcc gattcttata tcttcaagaa ttttctcaag
     3781 ctcattcatc tcataaatat aacatttacc cgcaaaaatt tcctattatt gcgtaattaa
     3841 aacataatac tatatgttcc attatttata cccacatcta cctgaatcta ttgatggtaa
     3901 ggaaattccg gcgggtctaa tctcaagaat ttgactgatg aataaaaata tgtagtataa
     3961 ggccagaaat cgttcgttgt caacaaaaac gttctactta aacaaggagg aatcgcatga
     4021 caattttcta cgtcgtagcc gcttctttag ttggtctctt gattactttt tgtaaattta
     4081 gaggggttac gattggaaac atcattgctc gttctgaggc ggtgcttgaa gaatatgccg
     4141 acgacaacga aaacaagatt cacgaacacg ctttaattgt tcgcaatcca aacgacaaga
     4201 gcagggatgc acgcgcaatt attgtatcgg aatcagacct ggcagaactc ctactcaaaa
     4261 aaattttcat cgggttaagc ctgctcttcc ccgctttcac acagctcttt tatttctgcg
     4321 gagggacaag cctgggcgtc acgggatctg tttttatgtc gctaattatg tcgacaatgg
     4381 tttcgatctt ttcagtcgca attctaaata aattttcccg ttttcacccg ctctgctttc
     4441 gcgaagaaag tttgtcttat atcgcattcg gacccaaatt ctctaagggc gtctcaacga
     4501 tcctcctcga tgcggccgaa tatgagctca agaaatatga tgaagaagaa gccgaagaaa
     4561 aagccattgc agaaatgccg gaaaataccg cagaagagaa aaaggcaaaa gctatcgcag
     4621 aggtcaagct caaaattaag aagatcccct ttcccaaaaa tcgaacgatc tttgggtaga
     4681 acgttcaaac cccgccattg gcggggtttt taatttgtct ctaactggat tgcttcgctt
     4741 cgctcgcaat gacaccaatt atacattatc ttttcgacat tatgctctgt ttgtatgcat
     4801 ctaggtggtt gagcgcgata cctgtcccct ttgcaacaca ataaagtgcg tcgtcggcaa
     4861 ggattgcttt tacaccggtc tttctataga taagctccgg gagatttgcg agcattgatg
     4921 tgccgcctgt cataattatt ccctggtcga tgatgtcggc ggaaagctcc ggtggagtct
     4981 cctgcaagac atcttttatc gctttcacga cctgcttcat ctcgttcgct atagcctttg
     5041 cgatctcatt agtcgtgata tctgctgttc ttggaagccc gctcaagaaa tctcgtcctt
     5101 taatcatcat tttctgctcc tcgtctacag gtatagccga acctactttt atttttattt
     5161 cctccgccgt cttgtcgcct atcgccaagt taaaagtctt cttgatatag tctgcaatcg
     5221 cctggtctac tttattcccg gcgcacttaa cggaatttgc atagacaatg cctccgagag
     5281 aaataacggc gcagtctgtc gtgcctccgc cgatgtctac aaccata
//
LOCUS       DAOTEX010000052         5201 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3118
            NODE_11523_length_5201_cov_3.448892, whole genome shotgun sequence.
ACCESSION   DAOTEX010000052 DAOTEX010000000
VERSION     DAOTEX010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868142
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5201)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5201)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 883
            CDSs (total)                      :: 833
            Genes (coding)                    :: 829
            CDSs (with protein)               :: 829
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5201
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11523_length_5201_cov_3.448892"
                     /isolate="AMR_MDS_3118"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            216..1406
                     /gene="ftsZ"
                     /locus_tag="P5056_03500"
     CDS             216..1406
                     /gene="ftsZ"
                     /locus_tag="P5056_03500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HRY62807.1"
                     /translation="MPKINPEIESFARIKVIGVGGSGGNAINHMINSKIDGVEFVTMN
                     TDGQDLQKSLAPKKVHIGKNLTHGRGTGMNPEIGKRAAEETLEEVQATTKGADMVFIA
                     GGMGGGTGTGAAPVIAKTAKDQGALTVAVVTKPFFFEGTQRMRLANEGLERLRNEVDA
                     LIVIPNDRILSVIDKTTTCTSAFAMCDEILRQAVEGISDLITTTGIINVDFADVKSIM
                     QNAGSALMGIGTGTGENRAETAAKAAINSPLLDISISGARGVLFAIAGGEDMTMFEIQ
                     EAAKIVTESIDHDAKVIFGAIRDSKLKKDEIKVTVIATGFPDERRGAPSKERISEYKS
                     TPITEKETEKPEPIKYEEETKHSPKKEEKIVFKEEEKEEVEEVESEDDKWSSVPAFLR
                     RSKK"
     gene            complement(1441..2175)
                     /locus_tag="P5056_03505"
     CDS             complement(1441..2175)
                     /locus_tag="P5056_03505"
                     /inference="COORDINATES: protein motif:HMM:NF016817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2817 domain-containing protein"
                     /protein_id="HRY62808.1"
                     /translation="MIYKTFLEKLRAVCLEKGLKLQLIGFAGNDKDFPIYKIVVPGTA
                     KNKKTIFFSAGIHGNEPAGPWAVIEFLKKFSPKKYRGPRMVIVPVMNPFGFENNKRRN
                     AVGIDINRHFRDSRLRGDNKLIYDNIKDENVFFFHSMHEDPDVKKFYAYGYEEKPEKI
                     YRDVVKVGGGFFKINKSKKIYGDVSENGLVLNDKGGSLENRLVHDRIPFILCTETPGK
                     EPFEKRTRANAKIMKKVLEFCIRNSD"
     gene            complement(2267..2605)
                     /locus_tag="P5056_03510"
     CDS             complement(2267..2605)
                     /locus_tag="P5056_03510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62809.1"
                     /translation="MSDKYYKISHYVTLATWVVLSSVILTIFFQNFFVVNLAPISLQN
                     TFAILFVIAVALFSATKLIIFLWNKVSDSRKSVVWINIFFSLFEAISFPLILFVGLHI
                     FAIISGVRVS"
     gene            complement(2659..2745)
                     /locus_tag="P5056_03515"
     tRNA            complement(2659..2745)
                     /locus_tag="P5056_03515"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2709..2711),aa:Ser,seq:cga)
     gene            2813..2884
                     /locus_tag="P5056_03520"
     tRNA            2813..2884
                     /locus_tag="P5056_03520"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2846..2848,aa:Glu,seq:ttc)
     gene            complement(4532..4798)
                     /locus_tag="P5056_03525"
     CDS             complement(4532..4798)
                     /locus_tag="P5056_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62810.1"
                     /translation="MATIDRQFNHTKLEIGEKQNGFLADKKVLLSCNSSTNQHRLLQK
                     ETLEALEELGGILKNIRKRMISEGYEIIDGTLHKVGSINLYGKS"
BASE COUNT         1541 a         1083 c         1032 g         1545 t
ORIGIN      
        1 caaaaataaa aaaggggcta gcctcgtggt ttagagaatt cttgccttaa ttttgcgttc
       61 tgctagtata aaacttgccc cgtcaccttc aagctctcgc ttgatgacaa agcttcagcc
      121 gcgggctgaa ttttattaca ccgatttaga ttatttttta taaaggtgtt tggggcatta
      181 tcctataaaa taaattaata acttagacta atttaatgcc taaaataaat cccgagatcg
      241 agtcgtttgc aagaattaaa gtcatcggag tcggaggatc cggaggaaac gcgatcaacc
      301 atatgatcaa ctcaaagata gacggggttg aattcgtgac aatgaatacg gatgggcaag
      361 atctgcaaaa atcgcttgct ccaaagaaag ttcacatagg caaaaatctt acccacggaa
      421 gaggaaccgg aatgaatccc gagataggaa agagagccgc ggaagaaacc cttgaagaag
      481 tccaggccac gacaaaagga gctgatatgg ttttcatcgc gggaggaatg ggcggaggca
      541 cgggcacagg agccgcaccg gtaatcgcca agacagctaa agatcaggga gcattaacag
      601 tggctgtagt cacaaagccg ttcttctttg agggaaccca gaggatgaga ctcgcaaatg
      661 agggactgga aagactcaga aatgaagttg acgcccttat cgtaatacca aacgacagaa
      721 tccttagcgt catagacaaa accaccacct gcacttccgc atttgctatg tgcgatgaga
      781 tcttgaggca agccgtagaa ggaatctccg accttataac caccacaggt ataatcaacg
      841 tagactttgc ggatgtaaaa tcaataatgc agaacgccgg ctcagccctt atgggtatcg
      901 gcacaggcac aggagaaaat agagccgaaa cagccgccaa ggccgctata aactccccgc
      961 ttctcgacat ctcaatttcc ggggcaagag gtgtcctctt tgccatcgca ggaggagaag
     1021 atatgacaat gtttgaaatt caggaggcgg ccaaaatagt gaccgagtcg attgaccacg
     1081 acgcaaaggt tatcttcgga gcaatccgcg acagcaagct caaaaaggat gagattaaag
     1141 ttactgtcat cgccacaggc ttccccgacg aaagaagagg cgcaccttca aaagaaagaa
     1201 tatccgaata caaatcaacc cctataacag aaaaagaaac cgaaaaacct gagccgatta
     1261 aatatgagga agagacaaaa cactctccta aaaaagaaga gaaaatagtc ttcaaggaag
     1321 aagaaaaaga ggaggtggaa gaagtcgagt cagaagacga taagtggtca tctgtgccgg
     1381 cattcctaag gaggtctaag aaataattta aaaaaatccc gccataagcg ggatttttta
     1441 ttaatcagag ttccggatac aaaattcgag aaccttcttc attatttttg cattcgccct
     1501 ggtccttttt tcaaaaggct ccttgcccgg ggtttcggtg cacaaaataa aaggaattct
     1561 atcgtgaaca agtctatttt ctaacgagcc gcccttgtcg tttaaaacga ggccgttctc
     1621 ggagacatct ccgtatattt ttttgctttt atttattttg aagaaaccgc cgccgacctt
     1681 aactacatcc cgatatattt tttcgggctt ttcctcgtag ccgtaagcgt aaaatttctt
     1741 tacatcagga tcttcgtgca ttgaatggaa gaaaaacacg ttttcatctt ttatattatc
     1801 gtatataagc ttattatcac ccctcaagcg agagtcgcga aaatgtctat ttatgtctat
     1861 tccgacagca ttacgtcttt tgttattctc aaaaccaaaa ggattcatga ccgggacaat
     1921 gaccattctc gggccacgat attttttggg tgaaaacttt tttaagaatt ctataacagc
     1981 ccatggcccg gcaggctcat ttccgtgtat tcccgccgag aagaaaatgg tcttcttatt
     2041 ttttgccgta ccgggaacaa caatcttata aatcggaaaa tctttgtcgt ttccggcaaa
     2101 accgataagc tgaagcttaa gccccttttc cagacagacg gcccttaatt tttcgagaaa
     2161 agtcttatat atcattgttt tttctatatt aacacaaaaa atgagaatag taaagaaacg
     2221 cgcgccaaac gctatctccc ccgaagatat aggcaggata ttaaatttaa gagaccctta
     2281 cgcctgaaat gattgcaaaa atgtgcaaac cgacaaaaag aataagcgga aaagatatcg
     2341 cctcgaataa actaaaaaat atatttatcc aaaccacgct ttttcgagaa tcggatacct
     2401 tgttccataa aaatataatt aatttcgtgg cagaaaatag agcaacggcg ataacaaata
     2461 aaatggcaaa tgtattttgc aaagaaatcg gagcgaggtt taccacgaag aaattctgaa
     2521 aaaatatcgt taatataaca gacgacaaga caacccaggt cgcgagagtc acataatgag
     2581 aaattttata atatttatct gacataattg tgtaacttaa ttatatcaga atcccgccct
     2641 tagcgcggaa tttttatacg gaggcgggag gatttgaacc tccgaggccg attaaggcct
     2701 actgcgtttc gaatgcagcg gtttcagcca ctcacccacg cctccatgcg gcatatatta
     2761 gcaggccttt gattttttta caaaaatttt atagtatatt gaaataagtt aaggccctat
     2821 cgtctaacgg ttaggacatc gccttttcaa ggcggtaatc agagttcgat tctctgtagg
     2881 gtcacaaata gcaaggaaac ggttttcaaa ggcttcaaat ccttttttga ccttgaattg
     2941 caatatatct tgagtaatag aaagttctga aaagattaca cgaacaatct gctctttttc
     3001 gtgtggttta gcgaattgat aatatacagc aacattttta atgagttctg aaagtttttg
     3061 aacatctttc attgtctcgt gcattgcaat atcagaggtt tgctcttcgg tctgtagtaa
     3121 agagagttca ctatcaagtt ttgtttcttc agtaacgtaa ctttctggtg tgtatactcc
     3181 agccttcaat aaaggtattt tgtttgaacg taggtatgca agatcttcac gtatcttttt
     3241 cttttttcgc tctaattgat cgagcgtttt gtgtcgattt tcttcacata aggcaatctc
     3301 cgtgcccaca cgagcatcca tctgctcaat ttcttcatcg gtaaagtgaa gacccgcaat
     3361 aagaccacta attttcttgt caataaaact aaagttaaaa ttcttcagtg tatttgggca
     3421 accttttacg caccgtgagt tgtaatattg tatacctttc ttcgtgtatg gcgtatatac
     3481 acgaacacaa agcgcacaac gacacacccc gcgcaacggc tggtccaatt tctctgtata
     3541 gtgaacactt actttctttt ttttgagtat agactgaaca ttatcgaaaa gatcatcgct
     3601 tacaatggct ttgtggctga tactttgtat atatttgcct tcagaattga gtgttctccc
     3661 agtataaaaa cgattagtga gtattctgct aatgtggttc tcattcactg gacgcgaggt
     3721 cttttcgatt tccttaacct catcttcttc agcaagcatc tcttcttttg tccttctgcg
     3781 cctcattggc atagtcgtaa aaccttgttc atttgcaaat cgagcaatat cagacaagct
     3841 ccagtctccc gtagcataat actcatacat ttgtttgatg attggcgcac ggaattggtc
     3901 aatgggtttg tcttccatct ttcccatatt aagataaccg attggcgcac gatatgtgca
     3961 gatacctctc tctcttgagt ttttgattgt gagtgtaact ttctcgctcg taactcgcga
     4021 gtgatgctct gcaaacatcc catcaatatc catatgcaga gcgccagaac tagacttctc
     4081 gtattttgca tatacaaagc gaaaatctac accattgcgc attaactttc gaaccaaggt
     4141 gtcgtcgccc ttatttctac tgatacggtc ccagcaaaga catacaactc ctttgaaata
     4201 tctttggctg acgaactgga gcatcttctg gaactttggt ctatcgatat gatattgagc
     4261 caaaccatca tctgtaataa ttatgtcgtc gttttcggta aatcctgagt gcttttcgga
     4321 aataatacca tcagcagaga aacccttgag ggtgatttgt gctatcggaa gtttttctcg
     4381 atgagcgtat ttagcattct ctgttttttg atatgaaatg gagtttttct gattgtccgc
     4441 ttcgtccgtg ctttttctgt tgtatatcaa ataacaatca cggtatttac cgcaaagtat
     4501 ttcatttatt aagtcttttt ccgttttaac cctaactttt tccatataaa tttattgaac
     4561 ctactttgtg aagtgtgcca tctattattt cgtatccttc tgaaatcatt ctttttcgga
     4621 tattttttag tatcccaccg agctcttcaa gggcctctag ggtttctttt tgtaaaaggc
     4681 gatgctgatt tgttgaagag ttacaactca ataatacttt tttatcagct aaaaatccat
     4741 tttgtttttc accaatttct aattttgtgt gattaaattg tcgatcgatc gttgccatat
     4801 ttcgttttta tttcacacac atctccgcct tccctaatga tagttgggcc acgggtctcg
     4861 gtatgtgcag acaaacggaa gatttgggga tgaactttca ggggcaaggc tgggacagtg
     4921 tttggctgta aaattaagaa aatccaccgt tcgtcctcct ttttctttat gttccgccct
     4981 catccttatc ttcagtagac aagatataca gattaggggg gcggtctacc gcccgcgttt
     5041 atctatatac cttgccgttt ccgttagatt gtttttattc cgaggcggtg gaaaattccc
     5101 gtcctcgtgc tgtctgtttg ttaggtcaaa ataaaaaact gacctgtatt aagtcagttt
     5161 atataaggtt attaaagtcg tataagacca aaagtggacc c
//
LOCUS       DAOTEX010000053         5003 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3118
            NODE_12131_length_5003_cov_4.229588, whole genome shotgun sequence.
ACCESSION   DAOTEX010000053 DAOTEX010000000
VERSION     DAOTEX010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868142
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 883
            CDSs (total)                      :: 833
            Genes (coding)                    :: 829
            CDSs (with protein)               :: 829
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5003
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12131_length_5003_cov_4.229588"
                     /isolate="AMR_MDS_3118"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /locus_tag="P5056_03530"
     CDS             complement(<1..576)
                     /locus_tag="P5056_03530"
                     /inference="COORDINATES: protein motif:HMM:NF013213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HRY62811.1"
                     /translation="MNSSPKFFDIHAHLHFSAYKDDRDAVLARTLPDTWVINIGSSKN
                     TSEDAVNLAHKYPEGVYAAIGLHPIHTDESFVDEHESERDLLETEFDYDFYKKLALDP
                     KVVAIGECGLNYELEITNDELWEKKRERQKEVFRRQIELAIEVNKPLMLHIRNAYEDA
                     LEILNSYFLIHNSRLRGNVHFFAGSLEVARKF"
     gene            complement(696..2120)
                     /gene="metG"
                     /locus_tag="P5056_03535"
     CDS             complement(696..2120)
                     /gene="metG"
                     /locus_tag="P5056_03535"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HRY62812.1"
                     /translation="MGKKTYITTTLPYVNADPHIGFALEIIQADIYARFLRERGGEVF
                     FNFGTDEHGQKIYEKAKENGEDTQAYVDRYAAKFDALKKALNLTYNNFIRTTDPHHIM
                     AAQEFWKRCNANGDIYKKNYKVKYCVGCETEKTDSDLVNGCCPDHSNRQLEIREEENY
                     FFRYSKYGQKLLDLYNNNPEFVVPSWRLNEVRKFVEGGLEDFSISRLRTKMPWGIDVP
                     GDPDHVMYVWFDALVNYVSAVGWPDRMEEFDSWWPVVQFAGKDNLRHQSATWQAMLMS
                     AGIAPSKKIFIHGWVLGGGGQKMSKSLGNVINPFEVVEKYGGDALRYFLSREMTPFED
                     GDFTEARFKEAFNAGLANGLGNTSSRVLKMAESYLPGPVTVGEAEIPALYSKYIEGLE
                     LNKAMDLIWSKLSEMDSIIQETQPFKLVKTDKDAAAKIVTDLVKRLFSISKMLAPFMP
                     ETSAKIEEAIKANNLAAPLFMRLE"
     gene            complement(2126..3199)
                     /locus_tag="P5056_03540"
     CDS             complement(2126..3199)
                     /locus_tag="P5056_03540"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HRY62813.1"
                     /translation="MKTEQDNTLKIEISSGVIFKTIALLLVLWLAFYLRDIVIAMLTS
                     IVVASAIEPLINWLTNRKIPRALAVVLVYAGLIGFVFLSISVFFPLIFDDFFKLFTIL
                     PAYIKSFDVSSYVSEAPGKILSALKNMIADSFSVEKIMPDIMGVISGTSSNIVDTAKA
                     IFDEVVMFVLVAVFSFYFAVQENGIEGFLRLVSPLKYENYILDLWKRSRRKIGLWMQG
                     QILLGVLIGVFVFLGLSIIGIKNALALAVFASVIEIIPVFGPILAAFPAVVLAFIQSP
                     SLGFFVISFYVIIQQFENHLIYPLVVRKIIGIPPLISILFLLVGWELVGFLGIVLSIP
                     AAVIIIEVIDDLEKKKRFVASVK"
     gene            complement(3212..4480)
                     /locus_tag="P5056_03545"
     CDS             complement(3212..4480)
                     /locus_tag="P5056_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRY62814.1"
                     /translation="MIYKKTTLKNGLRVITIPEEDSLAVAVFVLVEAGSKYEKKEING
                     LSHFLEHMCFKGTKTRPTALDISSELDSLGAQFNAFTSQEFTGYYAKVAANHFDKALD
                     IVSDLYLNPVIDSVELEKEKGVIVEEINMYEDLPQHRVQDLILEALYGDQPAGWNIAG
                     DKETVRKFDRETFLKYRGEHYVSSATTVVVAGNFNEEKAIKDIEEKFKGMHSGEKHGK
                     LAVIEAQKAPLVLTKKKESDQTHLVLAVRTFSATDKRNHTIDVLSQVLGGGMSSRLFQ
                     KVREEMGAAYYVRCSNNTHTDHGFLEASAGADNKRAKEVISAIVAEFARMKNELPTEK
                     ELNRAKESLIGNMFLSLETSDAIAGHYGLQEVIKREIEEPEEYAKKIRAVTGEEVMTV
                     AKEIFRQEHLNLALIGPAENKDDFVSLLKL"
     gene            complement(4576..>5003)
                     /locus_tag="P5056_03550"
     CDS             complement(4576..>5003)
                     /locus_tag="P5056_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010165002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HRY62815.1"
                     /translation="FLPHVIEPSLGVDRTVLAILTSAYTEDNMGEGTRAFLKFKPKVA
                     PIKAAVFPLMKNKPELVEKARDIWKNLKAVVPQIMFDDNGNVGKRYRRQDEIGTPFCI
                     TVDFDTLENDTVTVRDRDTGAQERVAVKELNNYILSKIS"
BASE COUNT         1389 a         1230 c          913 g         1471 t
ORIGIN      
        1 gaattttctg gcaacctcta agctaccggc gaagaagtga acgttgcccc taagacgaga
       61 attatgaatt aagaaatatg aattaagaat ttccaaagca tcttcgtatg catttctaat
      121 gtgtagcatg agcggcttat ttacctcaat tgccaactct atttgtcttc taaagacctc
      181 tttctgtctt tctcttttct tctcccataa ttcatcattc gtaatttcta attcgtaatt
      241 taacccacat tcccctatcg cgacaacttt tggatcgagt gcgagctttt tatagaaatc
      301 ataatcaaat tctgtctcca ataagtctct ttcagactca tgttcgtcga caaaagactc
      361 atctgtgtgt ataggatgaa gccctatcgc cgcgtaaaca ccttcaggat atttgtgtgc
      421 caagttaacg gcgtcctctg acgtattttt gcttgagccg atatttataa cccacgtatc
      481 aggtaaggtg cgcgccaaaa cagcatccct gtcgtcttta tacgcagaaa aatgaagatg
      541 ggcgtgaata tcaaaaaatt tcggagaaga attcataagg tctataaagt taattttcta
      601 gtgtcattcc gagcctgtcg aggaatccct tccgcaaatg cttttgaaaa tataagcgat
      661 ttctcgacaa gctcgaaatg acaacaacca agagtttatt ccaatctcat gaaaaggggt
      721 gcggctaaat tgttcgcctt tatcgcctct tctattttgg cggaagtctc aggcatgaaa
      781 ggcgcgagca tttttgaaat agagaaaagt ctctttacga gatcggtgac aatcttcgcg
      841 gcggcgtctt tatctgtctt aacaagcttg aaaggctgag tttcttgaat tatggaatcc
      901 atctcggaaa gcttgctcca aataaggtcc atcgccttgt ttaattcgag cccctcaatg
      961 tatttagaat atagagcggg tatttctgct tctcccacag ttaccggccc gggaagataa
     1021 gattctgcca tttttagcac cctgcttgat gtatttccaa gaccattcgc aagcccggcg
     1081 ttaaaagctt ccttaaacct cgcctctgta aagtccccgt cttcaaacgg tgtcatctct
     1141 cgcgacagga agtatcgaag cgcatctcct ccatattttt ccaccacctc aaacggattt
     1201 attacgtttc ccaagctctt tgacatcttt tgtcctcctc cgccgagcac ccagccgtga
     1261 ataaatatct tttttgatgg cgcgattccc gcagacataa gcatcgcctg ccaagtagct
     1321 gactggtgtc tcaaattatc ctttccggcg aactgaacta ccggccacca ggaatcaaat
     1381 tcttccattc tgtcaggcca cccaacggca gaaacatagt tcacaagagc atcgaaccaa
     1441 acatacatta cgtgatcagg atctcccggc acatctattc cccagggcat ctttgtcctt
     1501 agtcttgaga tactaaaatc ttccagccct ccttccacga attttctgac ctcatttagt
     1561 ctccatgacg gcacaacaaa ctcaggattg ttattataaa gatccaacaa cttctggccg
     1621 tattttgaat atctgaaaaa gtaattttcc tcctctctta tttcaagctg tctatttgag
     1681 tggtcggggc aacatccgtt cacaaggtca gaatctgtct tctcagtctc acacccgacg
     1741 caatatttaa ctttgtagtt cttcttataa atatccccgt tcgcattgca cctcttccaa
     1801 aattcctgcg cggccattat atgatgcgga tctgtggtcc tgatgaaatt gttgtatgta
     1861 agattgaggg ccttcttcag cgcatcgaat tttgcggcat acctatcgac gtaagcctgc
     1921 gtgtcttcgc cgttctcctt ggctttctcg tatattttct gaccgtgttc atcggttcca
     1981 aaattaaaaa agacctcgcc tcccctctca cgcaaaaaac gcgcgtatat atccgcctga
     2041 attatctcca gcgcaaaacc aatatgcgga tctgcgttaa cgtatggtaa cgttgttgtt
     2101 atataagtct tttttcccat aatttttact ttactgaagc tacgaatctt tttttctttt
     2161 ctagatcgtc tatcacctcg ataataataa ccgccgcagg tatagaaagc acaataccca
     2221 aaaatcctac gagctcccaa ccgacaagca agaagagaat agaaataagc ggaggaatac
     2281 cgataatttt tctgacaaca agcggatata tcaaatgatt ttcaaattgt tggatgatta
     2341 catagaaact tataacaaag aatcccaaag aagggctctg tataaaggcg agcaccaccg
     2401 cagggaacgc ggccaaaatc ggtccgaaga ccggaataat ctctatcacg ctcgcaaaaa
     2461 cggccaatgc cagggcgttc ttaataccga taatagaaag tcccagaaat acaaataccc
     2521 cgataaggac acctaacaaa atttgtccct gcatccaaag accgatcttt ctcctggatc
     2581 tcttccaaag atcaagaata taattctcat attttaaagg tgaaaccaat ctcaaaaatc
     2641 cctcaatacc attctcctga acagcaaaat agaaagaaaa cacggcgaca agaacaaaca
     2701 ttaccacttc atcgaagata gcctttgctg tatcaacaat attgcttgaa gtaccggaaa
     2761 tgacccccat aatatccggc ataatttttt ctaccgaaaa actatccgca atcatattct
     2821 tcaacgcaga taaaatcttg cccggcgcct ctgacacata gctggagaca tcaaaagatt
     2881 ttatatatgc cgggagaatt gtgaaaagtt tgaaaaagtc atcaaatata agcgggaaaa
     2941 atacagaaat ggacaaaaat acgaatccga tgaggcccgc atatactagc accacagcca
     3001 aggcacgggg tatcttccta ttggttagcc aattaatgag tggctcaata gcggaagcca
     3061 ccacgataga agtgagcatc gcgatgacaa tgtcgcgaag gtaaaaagca agccacaata
     3121 ccaaaagtag cgctatggtc ttgaaaataa ctccgcttga gatctctatt ttaagagtat
     3181 tgtcttgttc tgttttcacg cttttattgt attacaattt caatagactt acaaaatcat
     3241 ccttattttc cgcaggcccg ataagagcca aatttaagtg ttcttgtctg aatatttctt
     3301 tcgcaacagt catcacttct tctccagtga ccgcgcgtat ctttttggca tactcttccg
     3361 gctcttctat ttccctttta ataacctcct ggaggccgta atggcccgcg atagcgtcgg
     3421 aagtttcaag gctcaaaaac atattgccta tgaggctctc ctttgcacgg ttaagctctt
     3481 tttctgtcgg aagctcattc ttcatcctgg caaactcggc aacgatagcg gagataacct
     3541 ccttagccct cttgttgtcg gcaccggcag aggcctccaa aaagccatga tcagtatgtg
     3601 tattatttga gcaccttaca taataagcgg cgcccatttc ttctctcact ttttggaaaa
     3661 gtcttgagct cattcccccg cccaatactt gggaaagaac atctatggtg tgatttcttt
     3721 tatctgtagc ggaaaaagtt ctaacagcga gcacgaggtg cgtctgatcg gattctttct
     3781 tcttcgttag cacgagagga gccttctgag cttctattac agcaagcttt ccatgcttct
     3841 ccccggagtg catgccctta aatttctcct ctatatcttt tatcgctttt tcttcattaa
     3901 aatttccggc gacgacaacc gtggttgcgc tagacacata atgctcgccc ctatacttca
     3961 aaaaagtttc tctatcaaat tttcttacag tctctttgtc ccccgcaatg ttccacccgg
     4021 caggctggtc gccataaagc gcctccaaaa taaggtcttg caccctgtgc tgaggcaagt
     4081 cttcatacat atttatctct tccacgataa cccctttttc tttttctaat tccacagagt
     4141 caataaccgg attcaaatat aaatccgaaa cgatatctag tgccttatca aaatgattcg
     4201 cggccacttt agcgtaatag cccgtaaatt cttgactcgt aaacgcgttg aattgagcgc
     4261 cgagagaatc gagctctgaa cttatatcaa gcgctgtcgg acgagtcttt gtgcccttga
     4321 aacacatatg ttctaggaag tgcgagaggc catttatttc ttttttctca tactttgagc
     4381 ccgcctctac gagcacgaag acagcgacag ccaagctatc ctcctcggga atcgttatca
     4441 ctctgaggcc gttctttaga gtagtttttt tatatatcat agtatgttaa tttctgtcat
     4501 cgcgaacgaa gtgaggcgat ccagaattcc accgacagca tggattgccg cgcttcgctc
     4561 gcaatgacaa taaatttaag agatcttact caaaatataa ttattcagct ccttgaccgc
     4621 taccctttcc tgcgcgccgg tatctctgtc tcttactgtg accgtgtcat tctcaagagt
     4681 gtcaaaatct actgttatac agaaaggagt tccaatctca tcctgtctcc tgtatctttt
     4741 tccaacgttt ccattatcat cgaacattat ttgagggaca acagccttca agtttttcca
     4801 aatatctctg gctttttcta caagctccgg cttatttttc ataagaggga aaacagcggc
     4861 ctttatcgga gcaactttcg gtttaaattt caaaaacgct cttgttcctt cgcccatatt
     4921 atcctccgta tatgcgctgg tgagaattgc gagcaccgtc ctatctacac caagtgacgg
     4981 ctctatcacg tgcggcaaga att
//
LOCUS       DAOTEX010000054         5002 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3118
            NODE_12139_length_5002_cov_4.613907, whole genome shotgun sequence.
ACCESSION   DAOTEX010000054 DAOTEX010000000
VERSION     DAOTEX010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868142
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 5002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 883
            CDSs (total)                      :: 833
            Genes (coding)                    :: 829
            CDSs (with protein)               :: 829
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5002
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12139_length_5002_cov_4.613907"
                     /isolate="AMR_MDS_3118"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..472
                     /locus_tag="P5056_03555"
     CDS             <1..472
                     /locus_tag="P5056_03555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62816.1"
                     /translation="AGELNSFRGATNSDFETYAKGLVVSLKPYARPDLPNEGDITLSA
                     LESGDADGLKNVLLMAQIHYAIANELKTLKVPEEIKFRHLFLINNIETLSYADGLMVN
                     AFDDPKMGIEAVNLYQSATKGLMNSLADINDFFKTKGIVFSQDEKLKIYINTLQ"
     gene            496..3093
                     /locus_tag="P5056_03560"
     CDS             496..3093
                     /locus_tag="P5056_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62817.1"
                     /translation="MNKEKVFKILIKKSRLNTFSVLLSSSLFISSALIFPIYASASTL
                     PMGNSAGIDQAKQFLETIRTQIKFNLPTQDAAAAQIQSLLEQNLVSALGNIDGLGQKL
                     ESASSIINLANSVNKGNFSTIIPQILSQASNLQGLQGITNIGSITNQIQSLSSKIPGL
                     SSSLGTINKDLANLSNVNSVVSNLSTKLNNVTSVAGNMQSALQTGASRTALGSYVSSG
                     LDANGLTADEAARYASYEEYWNGLESAWNETDSALAGAASLGDINSISSNFSNANAAG
                     MDSGQQTVCKPNSANNKAGGNGNGVPTIEQEGPLLDYTGQLVNYTAKIKAINEETCKL
                     VEQLYKKEFELDKKATEDAQKAVAEAKKEFEKQFAEGRKPANDLSTKKSSATTPKELE
                     GEEKEAVRNNQINNLKNTSISKKYQDVLSEELKKQNEIEKDDNAAYAARVAPTFDTDK
                     FFEDTEAIKKDDYFSKLSEALYPKNTLIGQYFIQMAEKQRAEEEAVERARTEYIAGQG
                     IIGVKQCIKEITTEDGKTSCLDWQVTIPAKTYGDLASAYFTDNYKQTSGVKEDSDKAN
                     IPAAQQDVQVIATAGGAAGGGGTGGNTTSSNTSGGTSEANTTNPTNNQNFNLEDLLNQ
                     IGEGSSEENQGETSSVSIPLPTISFSAEKASPVNGVSVTRISWSADLALSCRAANNWI
                     WYSDKATTTRDILVKQDADRSLDGNVPIYHPAAFSFSATVTGNDRLINIPQAYTLSIV
                     KEGTGFKQTVRYEPNITAELLENDVFNIKINNDLANDPTISILFTDGMTKSELVSQII
                     SVMNNTDQATDAGKIYKGIAKEKSADGNALILTKSVSNVPATADYEIACKNITDKETT
                     KKITIEFFK"
     gene            3097..>5002
                     /locus_tag="P5056_03565"
     CDS             3097..>5002
                     /locus_tag="P5056_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62818.1"
                     /translation="MNFWRKLIIAYALLVVVFANINISYAQTNADTSVTTNTQQTGGD
                     SSQSSEEPAAGAKTACDTHWTYFNIDCLTWLIAEILGMVLYLFSLLLSLAVWAFDASI
                     NLSINDLHIYMGSDSSVVAAWKILRDVINMIFLLVMMYIAVGTVLQLEGVNWKKQIGA
                     IIMAAVMINFSLAVTRIIIDTTNVFALYFYEKAQGPADSKTGERQGIANLIFERLAVP
                     DALKEDTGSLENSPSLGAIIVNFLGSILVLLSASYVFFLAAFLFVFRSVALIFSAIFS
                     PLPWIGMAIPKMGGKLSSSWWDSLINNAVFAPVYTFCLYFAIQFILCAPLDKIQGGDK
                     GGILPLGMFFVITISLLFGCVMVAQKMGAQGMSVAQSGAKWVMGASLGTAGAATAWTL
                     KKTGVNDLAKGAEGRVTGALKPITTFMNKADLGKAITTKTGMGYKAVKESITSPLSSV
                     TKAVKMGTGIEIMPSDADWKAQKKAEKEYLAEQELAAKKDRLIEISKMTEEQIAETAT
                     KEGKEEDDKEREIGKEIQELLENFRGDKIMDIGLKNLKAQNGRIASWINEAQIKAVEN
                     HADTKPEDMQAIQNAIYENKANVRGIRAVEVDVVSRNWSEWDKEKKEEAITNMGSRHV
                     ADLSDTILGSD"
BASE COUNT         1714 a         1033 c         1128 g         1127 t
ORIGIN      
        1 cgccggcgag cttaactctt ttcgcggcgc cacaaattcc gattttgaga catacgcgaa
       61 aggactggtt gtctcgctta agccatacgc cagaccggac ttgccaaacg agggggacat
      121 aaccctctct gcgctagaaa gcggcgatgc ggacggatta aagaatgtcc tgcttatggc
      181 ccaaattcat tatgcaatcg caaacgaact caaaacacta aaagttccgg aagaaataaa
      241 gtttagacat ctttttctga taaacaatat agaaacatta tcttatgccg acggtctgat
      301 ggtaaatgca tttgacgatc cgaagatggg aatagaagcg gttaatttat atcaatcagc
      361 gacaaagggt ttaatgaatt cgcttgcgga tataaacgat tttttcaaaa caaaaggaat
      421 tgtgtttagc caagacgaaa agctaaaaat ttatataaac actctgcaat aggaaggaag
      481 taagaattaa gaataatgaa taaagaaaaa gtttttaaaa tattaataaa aaaatcaaga
      541 ctaaacactt ttagcgtctt attgtcgtca tccttgttta tttcaagtgc tttgattttc
      601 cctatctacg cctccgcttc aacattgccg atgggaaatt ccgccggaat agaccaggcg
      661 aaacaattct tagagacgat cagaactcaa ataaaattta atttaccgac gcaggatgcc
      721 gcggccgcac aaatccagtc cctcctagaa caaaatctcg tcagcgccct gggcaatatc
      781 gacgggttgg gacaaaaact agaaagcgca tctagcataa taaacctggc aaacagcgta
      841 aacaaaggta atttctctac aatcataccc cagattttgt ctcaagcgag caatcttcaa
      901 ggactacaag gtataacaaa cataggatca ataaccaacc aaatccaaag cctttcttca
      961 aaaatcccgg ggcttagctc atctttgggc acgataaaca aagacctcgc gaatctcagt
     1021 aatgtaaata gcgtcgtaag caatctgagc acaaaactta acaatgtgac gtccgtagcg
     1081 ggaaatatgc aaagcgctct gcaaacggga gcgtcgagaa ccgccctcgg ctcatatgtt
     1141 tcaagcggac ttgatgcgaa cggcctaacg gcagatgagg cggcgagata cgcgtcatac
     1201 gaagaatatt ggaacggact ggaaagcgca tggaatgaaa cagactccgc tctagcaggc
     1261 gccgcttcgc tcggagatat caatagcatc tcttctaatt tttctaatgc aaacgccgcc
     1321 ggtatggata gcggacagca aacagtctgc aaaccaaact ccgcgaacaa caaggccgga
     1381 ggcaacggca atggagttcc gacgatagaa caagaaggac cactcttaga ctatactggc
     1441 cagcttgtta attacaccgc gaaaataaaa gcgataaacg aagagacctg taaactcgtt
     1501 gagcaattat ataaaaaaga atttgaactc gataaaaaag cgaccgaaga cgcccaaaaa
     1561 gccgttgctg aagcaaaaaa agaatttgaa aaacaatttg cggaaggcag aaagcctgcg
     1621 aacgacctga gcacaaagaa atcatcagct acaacaccga aagaactcga aggggaggag
     1681 aaggaagctg taagaaacaa tcaaataaac aatctcaaaa acacgagcat tagcaaaaaa
     1741 tatcaggacg ttctcagcga agagcttaaa aaacaaaacg aaatagaaaa agacgataat
     1801 gccgcttatg ccgccagggt tgctccgacg tttgatacag ataaattctt tgaagacaca
     1861 gaggcgataa aaaaagacga ttatttcagc aaattatccg aagctttata tccaaaaaat
     1921 actcttattg gacagtattt tatacagatg gccgagaagc aacgagctga agaagaagct
     1981 gtggaaaggg cacgaactga atatatagca ggacagggta tcattggagt taaacagtgt
     2041 attaaagaga tcaccacaga agacggcaag acgagctgtc tcgattggca agttaccatt
     2101 cccgccaaaa catatggaga tcttgctagc gcatacttta ccgacaacta caaacaaact
     2161 tccggcgtaa aagaagattc cgacaaggca aatattccgg cggcacagca agatgtgcaa
     2221 gtaatagcca cagcgggtgg agctgcagga ggagggggga ccggaggaaa tacaacatct
     2281 tctaatacaa gcggaggcac ctccgaagca aacaccacaa atcctacgaa taatcagaat
     2341 ttcaacctgg aggatttatt aaatcaaatt ggagaaggaa gtagtgagga aaatcaggga
     2401 gaaacatctt ctgtaagcat tcctcttcct acaataagtt tcagcgcgga aaaggcatca
     2461 ccggttaatg gtgtaagtgt gacgaggata tcgtggtctg ccgacctcgc gctttcatgt
     2521 agagcggcga acaactggat atggtattca gataaagcta caactacacg cgacatatta
     2581 gtaaaacaag atgcggacag atcgctagac ggaaatgtgc cgatatacca tccggccgcg
     2641 ttttctttct cggcgacagt tacagggaac gatcgtttaa taaacattcc tcaggcatac
     2701 acactctcta tcgttaaaga ggggacaggt ttcaaacaaa ccgtcagata tgagccgaac
     2761 atcacggcag agctacttga gaacgatgta tttaatataa aaatcaacaa cgacctggca
     2821 aatgacccga caatctccat attattcacc gatggaatga caaaatcaga attggtctct
     2881 caaataatat ctgtaatgaa taatactgac caagcgacag atgccggcaa gatatacaaa
     2941 gggatagcaa aagaaaagtc tgccgacgga aatgctctta ttcttacaaa atcggtttca
     3001 aacgtccccg ctaccgccga ctacgaaata gcctgcaaaa atatcacaga caaagagaca
     3061 acaaagaaaa taacaataga gttttttaaa taaaaaatga atttttggag aaaattaata
     3121 atagcctacg ccttattggt agttgttttt gccaatataa atatttctta cgcacaaaca
     3181 aacgcggata cttctgttac cacaaataca caacaaacgg gaggggatag ttcacagtca
     3241 tcagaggaac cggccgcggg tgccaagaca gcatgtgata ctcactggac atactttaat
     3301 atagattgtc tcacctggct catagcagag atccttggaa tggtccttta tcttttttct
     3361 ctacttttaa gccttgcggt atgggcgttt gatgcgagca tcaatctttc cataaacgat
     3421 cttcatatat atatgggttc tgattcttcc gtcgtggcgg catggaaaat attacgagac
     3481 gtaataaata tgatattcct cctagtgatg atgtatatcg ccgtgggcac cgtccttcag
     3541 ctagaagggg tcaattggaa aaaacaaatt ggcgcgataa ttatggcggc ggtaatgatc
     3601 aacttcagct tggccgtaac gaggatcata atagacacaa cgaacgtttt cgcattgtat
     3661 ttttatgaaa aggcgcaagg gccggcagac agcaaaacag gcgaaaggca gggtattgct
     3721 aaccttattt ttgaaagatt agcggttcct gatgctctta aagaagacac cggctctcta
     3781 gaaaacagtc catctctcgg agcgataatc gtcaacttcc taggaagcat tttggtgctt
     3841 ttgtcggcct catatgtatt cttcttggcc gcattcttgt tcgtattccg gtcggtcgcg
     3901 ctcatattct cggcgatatt ttctcctctt ccatggatag gaatggccat cccgaagatg
     3961 ggcggtaaat tgagcagcag ctggtgggat tctctcataa acaacgcggt attcgccccc
     4021 gtatatactt tctgtcttta cttcgctata caattcatac tctgtgctcc gctggacaaa
     4081 atacaaggtg gagacaaggg cggaatatta ccattaggaa tgttttttgt aatcaccata
     4141 agcttactct tcggttgtgt gatggtggcc cagaaaatgg gtgcgcaggg aatgtctgtg
     4201 gcacaatcag gcgctaaatg ggtaatggga gcctcattag gaacagcggg ggccgcaaca
     4261 gcgtggacgt taaaaaagac cggggtgaac gatctggcaa agggagctga gggcagagta
     4321 accggcgcgc tcaaaccaat aacgacgttt atgaacaaag cagatctcgg aaaagcaatt
     4381 accacaaaaa ctggcatggg atataaggca gtaaaagaat caataacctc acctctttct
     4441 tccgtgacaa aggccgttaa aatgggaact ggcatagaaa ttatgccaag cgacgcagat
     4501 tggaaggcac agaaaaaggc cgaaaaagaa tacctggcag aacaagaact tgccgccaag
     4561 aaagatagac tcattgaaat atctaagatg acagaggaac aaatcgccga aaccgcaacc
     4621 aaggaaggaa aggaggagga tgacaaggag agggaaattg gtaaggaaat ccaagaactt
     4681 cttgaaaact tcagaggaga caagattatg gatatcggct taaagaattt gaaggcccag
     4741 aacggcagaa tagcgtcatg gataaatgag gctcagataa aggcagtaga aaatcatgcc
     4801 gatactaaac cggaagatat gcaggctatt cagaatgcta tatatgaaaa taaggccaac
     4861 gtcagaggta ttagagctgt agaggtagac gtcgtttcaa ggaactggtc agaatgggat
     4921 aaagagaaaa aggaagaggc tattacgaat atgggttcaa gacatgttgc cgatctttcc
     4981 gacacaatac ttggttcaga tg
//
LOCUS       DAOTEX010000055         4914 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3118
            NODE_12433_length_4914_cov_4.682445, whole genome shotgun sequence.
ACCESSION   DAOTEX010000055 DAOTEX010000000
VERSION     DAOTEX010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868142
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4914)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4914)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 883
            CDSs (total)                      :: 833
            Genes (coding)                    :: 829
            CDSs (with protein)               :: 829
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4914
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12433_length_4914_cov_4.682445"
                     /isolate="AMR_MDS_3118"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            297..2615
                     /locus_tag="P5056_03570"
     CDS             297..2615
                     /locus_tag="P5056_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62819.1"
                     /translation="MSKKFTFAVLAMFIACLGFAVNTNTAAAADILSVTAANVDQNGN
                     ALGGQVALGAGWQSTPHTYENITDGTTFFAKGSFGGLSNAGVYVIVKKDTTIKVDVET
                     NKVVSTVSTPGISKVEFVFNVLPITVATVDQNGNPLSGTVMVSNLLAWSASPQTIQNV
                     PSGVKTSFYAKYNALSGSMAGTIIGADTSYAINISTGVVTKTATPGVNKVEFVFEVLS
                     SITAANVDQNGNALGGQVALGAGWQSTPHTYENITDGTTFFAKGSFGGLSNAGVYVIV
                     KKDTTIKVDVETNKVVSTVSTPGISKVEFVFNVLPITVATVDQNGNPLSGTVMVSNLL
                     AWSASPQTIQNVASGAKLTAYAKYDVISVAGTAYTAAKDTTTKIDAETREITSVSTPG
                     INKVEFVFKSATPADTIPPVTTITISSAAGQNGWYIAPVEVSLTASDEGGSGLKETRI
                     CVDQNDLCVPVAGNSAEINAEGKNYFRYFSLDSAENSEVIKSETVNIDNTAPVSSVLA
                     EDTDNDTLLNTSSVSLSATDSGSGVLAIKYSFDNGEIFTTSSNPATVEIPAGLHVLTL
                     FSVDNAGNTEAVQNKTYNSPDACPSKEGKIENNGCPMAIVANVTVITHDQSKGGICGT
                     LPNGKPRPNCKLPLTNSEVKVFDMSDSGFINAFGAGRLNHKAAEIFASQNGLAGSCTT
                     NESGICKVGVGNTGKYFTVAKMVDGSNEVYAGKESDLFSDSAETEIIFSKFIDKSGDV
                     EYKHEGLALVPIVQGKWPFMLAFLGSLFSKIL"
     gene            2743..2937
                     /locus_tag="P5056_03575"
     CDS             2743..2937
                     /locus_tag="P5056_03575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62820.1"
                     /translation="MNSKLVWTIIVLLIVYGGYLMMTKTTTPVVETPAATEEVVATTT
                     TEAAADVAPETQEAAVPAAL"
     gene            complement(3048..3650)
                     /gene="pth"
                     /locus_tag="P5056_03580"
     CDS             complement(3048..3650)
                     /gene="pth"
                     /locus_tag="P5056_03580"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HRY62821.1"
                     /translation="MFIIVGLGNPGEEYENTRHNMGRVVLENMKKIFSKDSDFSDWET
                     DKKLRALTCEGKIGKQKVLFVEPDNYMNNSGGSVKPLITNKKKAEELIVIHDDLDLPM
                     GKYKITFNRGSGGHKGVESIIKNIKTEGFVRYRIGISPVTPSGKIKKPSGEEAVTKMI
                     MGKFREPEMAEIKKMSKRLAEATEMMIVEGRDKAMSLYNN"
     gene            3822..4433
                     /gene="lepB"
                     /locus_tag="P5056_03585"
     CDS             3822..4433
                     /gene="lepB"
                     /locus_tag="P5056_03585"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HRY62822.1"
                     /translation="MTLKNNDKNEAMSPIPCEEKPESWWDFIKEIIRFTILSLVVVLP
                     IRFFVAQPFLVDGASMVPTFEDGQYLIVDEISYYFNSPQRYDVIVFRYPLLPSKYFIK
                     RIIGLPGETVIVRDNTITIKNSENSEGITLEEVYVKNPDFQSRNKEWKLSDSEYFVMG
                     DNRAGSSDSREWGPLDKKFITGHVFVRLLPLSKAAIYPEKVTE"
     gene            4469..>4914
                     /locus_tag="P5056_03590"
     CDS             4469..>4914
                     /locus_tag="P5056_03590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HRY62823.1"
                     /translation="MEKKKASAKKVPPQAPKGMRDLIGDDFYNYLGFFEKSAEIAEYY
                     GFKPIETPILEETDLFTSGVGEGTDIVEKEMFTLKTKGGDSLTLRPEGTAPVVRAYME
                     HGMQSMPQPLMFYYYGQMFRHEKPQRGRLRELRQFGLEILGTDKSIA"
BASE COUNT         1489 a         1067 c         1047 g         1311 t
ORIGIN      
        1 cctctaaaaa tggcacaaaa tactatttcc cctggtgcga cggcgtaaag aaaataagcc
       61 ctaaaaacat agtagaattc tcttccgcag aggaggctga gtctgcgggc tttacaaaag
      121 ccgcaaattg ctccgggctt tagacaacaa ttctgacggc ctgtttatcc actacttttt
      181 ctccccaaaa acacttataa tatataagtt gcactttaaa aattaaaaag tgaagcaatt
      241 tttggtggcg tattattaaa gtgtattatc aaaaggtgtt tttattaaaa aataatatga
      301 gtaaaaaatt tacttttgca gtgctggcta tgtttatcgc ctgcctcggt tttgctgtga
      361 atacaaacac agcggcggca gccgacatcc tttccgtcac cgcagctaac gtagaccaaa
      421 atggtaatgc gttaggaggg caggtcgcgc taggtgcagg ttggcagagc actccgcaca
      481 catacgaaaa cataacagat ggaactactt tttttgccaa gggatctttc ggggggctaa
      541 gcaatgcggg tgtttatgta atcgttaaaa aagacactac gataaaagtt gacgtggaaa
      601 caaataaagt agtttccaca gtatcaacac cgggaataag caaggttgag tttgtgttta
      661 atgtacttcc tatcacagtg gcaaccgttg atcaaaatgg taatcctcta tcgggaacgg
      721 ttatggtttc aaatcttctg gcatggtcgg cttctcctca aaccattcaa aatgttccgt
      781 caggagtaaa aacatcattc tacgccaaat ataatgccct ttccggttcc atggcgggaa
      841 cgataatcgg agcagatacc tcttatgcta ttaacataag cacgggcgtg gtcacaaaaa
      901 cagcgacacc gggcgtaaac aaagttgagt ttgtatttga ggtcctttct tccatcaccg
      961 cagctaacgt agaccaaaat ggtaatgcgt taggagggca ggtcgcgcta ggtgcaggtt
     1021 ggcagagcac tccgcacaca tacgaaaaca taacagatgg aactactttt tttgccaagg
     1081 gatctttcgg ggggctaagc aatgcgggtg tttatgtaat cgttaaaaaa gacactacga
     1141 taaaagttga cgtggaaaca aataaagtag tttccacagt atcaacaccg ggaataagca
     1201 aggttgagtt tgtgtttaat gtacttccta tcacagtggc aaccgttgat caaaatggta
     1261 atcctctatc gggaacggtt atggtttcaa atcttctggc atggtcggct tctcctcaaa
     1321 ccattcaaaa tgttgcatcg ggagcaaaat tgaccgcgta tgcgaaatac gatgtgataa
     1381 gtgtcgccgg cacagcttat actgcggcca aggacacaac aacgaagatc gatgccgaaa
     1441 ctagagaaat aacttctgtg tcaacaccgg gaataaataa ggttgaattt gtctttaaat
     1501 cagcgacacc cgcagacaca attcctccgg ttaccacaat cactatatct agcgcggcgg
     1561 gacagaatgg ctggtatatc gcgccggttg aggtttcgtt aaccgccagc gatgagggag
     1621 gttccggatt aaaagaaacc aggatttgcg ttgatcagaa tgacctttgc gtaccggtcg
     1681 ccggaaattc ggcagagata aatgcggagg ggaaaaatta ttttagatat ttttctttgg
     1741 atagtgccga aaattctgaa gtaatcaagt cagagacggt taatatagac aacaccgccc
     1801 ctgtttcttc tgttcttgcg gaagatacag ataacgacac tcttctaaac acgagcagtg
     1861 tatcactatc agccacagac tcgggaagcg gagttttggc gattaaatat tcttttgaca
     1921 acggagagat tttcacaact agctcaaacc cggcaacggt ggaaataccc gcgggtctac
     1981 atgtattgac attgttctct gtagacaatg ccggcaacac agaggccgtc caaaacaaga
     2041 cctacaactc ccctgacgca tgcccgtcaa aagagggtaa aattgaaaat aacggttgtc
     2101 cgatggccat tgtcgccaat gtgacagtta tcactcacga ccaaagcaaa ggtggtatct
     2161 gcggcaccct tccaaatggc aaaccgagac caaattgcaa acttccgctt acaaattcag
     2221 aagtgaaggt cttcgatatg tccgactccg ggtttataaa cgccttcggc gcgggaagac
     2281 tgaatcataa agccgctgag atattcgctt cgcagaatgg gctcgcggga tcttgcacaa
     2341 caaatgaatc cggcatctgc aaagtcggcg ttggaaatac gggaaaatat tttaccgtag
     2401 caaaaatggt tgacggctca aatgaggtgt atgcggggaa agagtctgac ttgttttccg
     2461 actccgcaga aacagaaata atattttcaa aattcataga taaaagcgga gatgttgaat
     2521 ataagcacga gggtcttgct ctagtgccta tagtccaagg caaatggccc tttatgcttg
     2581 ccttcctcgg atcccttttc tcaaaaatat tataaacacc gagcacacaa aacagccctc
     2641 attattgagg gctgttttct tgattgcatt tttaaccaaa agtgctatta tcttaagtct
     2701 aagttattaa aggtcgggaa gatttttaaa acaaaaaaac aaatgaattc taaattagtt
     2761 tggacaatta tcgtcctttt gatcgtctac ggtggttacc ttatgatgac aaaaacaaca
     2821 actccggttg ttgaaactcc tgccgctaca gaggaagttg ttgctacaac aactactgaa
     2881 gcagccgcgg acgttgcccc tgaaacacag gaagcagccg ttccagcagc tctttaatta
     2941 aactgcaaaa caaaaaacac ccagcacaaa atgggtgttt tttgttttcc ttaattggta
     3001 atttgcctgc ctgccgtgcc tgcgcacgca gggcaggcag gtaatttcta attgttgtac
     3061 aggctcattg ccttatcgcg cccttcgacg atcatcattt ccgtagcctc ggcaagcctt
     3121 tttgacattt tctttatctc agccatctcc ggttccctga atttacccat aatcattttt
     3181 gtcaccgcct cctcaccgct aggtttctta atcttgccgg aaggtgtaac cgggctaatt
     3241 cctatcctgt atctgacaaa cccctcggtc ttaatatttt ttattattga ttccacccct
     3301 ttgtgcccgc ctgagccacg attgaatgtg atcttatact tacccatcgg gagatccaag
     3361 tcgtcgtgta ttacgataag ttcttcagct ttctttttgt ttgttataag cggcttaacg
     3421 ctccctccgg aattattcat ataattatcc ggctcgacaa atagcacctt ctgcttgccg
     3481 atcttcccct cgcaagtaag ggcgcggagc ttcttgtctg tctcccaatc tgaaaaatca
     3541 gaatctttgg agaatatttt cttcatattt tcaagcacca cacggcccat attgtgcctt
     3601 gtgttctcat attcttctcc aggattcccc agaccaacga taataaacat aaaaaaaatg
     3661 aattaagaat tatgaataaa gaattaagga tattctacaa tttatcaaat aaaaataata
     3721 gacctcgaac aaaacatatg ggcccacaat aaagaaatcg agatttttat ctctaattcc
     3781 taattcttga ttctttattc tttttgtagt ataattgcaa aatgacattg aaaaataacg
     3841 ataaaaacga ggctatgtcc cctattccgt gtgaagaaaa accggaatca tggtgggatt
     3901 ttataaaaga aataataagg tttactatac tctcgctggt cgtcgtatta ccgatacgat
     3961 tctttgtcgc gcaacctttt cttgtagacg gtgcgtctat ggtgcccaca tttgaagatg
     4021 gtcagtattt gatcgtcgac gagatctctt attacttcaa cagcccgcag agatatgatg
     4081 taatcgtttt cagatatcct cttctaccct caaaatattt tataaaaaga atcatcggcc
     4141 ttccgggaga aaccgtaatt gttagagaca atactataac aataaaaaat tccgagaact
     4201 ccgagggaat cactctggag gaggtttatg ttaaaaatcc ggatttccag agcagaaata
     4261 aagaatggaa attatccgac agcgaatatt tcgtaatggg agacaaccgc gccggcagtt
     4321 cggactccag ggaatggggg ccgctagata agaaatttat caccgggcat gtatttgtga
     4381 gacttctccc tctcagcaag gccgccatat atccggaaaa agtaaccgaa taaaataaca
     4441 attattaata attagagggt aatataaaat ggaaaagaaa aaagcatcag caaagaaagt
     4501 tccaccgcaa gcacccaagg gcatgagaga cctcatcggc gacgattttt acaactatct
     4561 tggttttttt gagaaatcag cagagatagc ggaatattat ggtttcaaac cgatagagac
     4621 tcctatctta gaagaaaccg atctcttcac ttcgggagtt ggagagggca cagatatcgt
     4681 agagaaagaa atgttcaccc tcaagacaaa gggcggcgat tctcttaccc tccggccgga
     4741 aggcacagcc cccgtcgtgc gtgcatatat ggaacacggg atgcagagca tgccacagcc
     4801 tcttatgttc tactattatg gacagatgtt caggcacgag aaacctcagc gaggcaggct
     4861 gcgagagctt agacaattcg gccttgagat actcggcacg gacaaaagca tcgc
//
LOCUS       DAOTEX010000056         4911 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3118
            NODE_12443_length_4911_cov_4.017504, whole genome shotgun sequence.
ACCESSION   DAOTEX010000056 DAOTEX010000000
VERSION     DAOTEX010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868142
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4911)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4911)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 883
            CDSs (total)                      :: 833
            Genes (coding)                    :: 829
            CDSs (with protein)               :: 829
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4911
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12443_length_4911_cov_4.017504"
                     /isolate="AMR_MDS_3118"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /gene="mltG"
                     /locus_tag="P5056_03595"
     CDS             <1..479
                     /gene="mltG"
                     /locus_tag="P5056_03595"
                     /inference="COORDINATES: protein motif:HMM:NF014656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HRY62824.1"
                     /translation="DVAKRLRVKFEEKYSEFSKEAAKQNIKWTTVVRLASIIQREAAG
                     KEDMPLISGILWNRLDKGVKLEVDASIQYIRGDVGKGFWAPISVSDKKIDSPYNTYMY
                     KGLPPHPICNPGAEAIKAVLYPEKTACIFYLHDPLGKIHCAKDYSEHLKNIKEFLK"
     gene            520..1902
                     /gene="asnS"
                     /locus_tag="P5056_03600"
     CDS             520..1902
                     /gene="asnS"
                     /locus_tag="P5056_03600"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012095705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HRY62825.1"
                     /translation="MTNKDIFREGEKLIGSKISVNGWVRTARSSGTFGFIELNDGTFF
                     KNLQVVFDDKLSNFKEMEKVRIGSALRIEGEVVKSPAKGQAFELKAEKIDMVADSDES
                     FPLQKKRHTFEYLRTIAHLRPRSNTFSAVFRVRSLAAFAIHKFFNEKGFVYVHTPIIT
                     GSDAEGAGEMFHVTTMDLKNPTKDDKGNIDYSKDFFGKETNLTVSGQLEGETFALAFN
                     KIYTFGPTFRAENSNTKRHAAEFWMIEPEIAFAGLKEDAELAEEMMKYIIKYVLDNAP
                     EEMEFFNKFIDTTLMDRLQNVLNSSFQKVTYTEAVKLLEESGAKFEYPVGWGKELQTE
                     HERYLTETIFKKPVIVTDYPKDFKAFYMRQNDDGKTVAAMDILVPGIGEIIGGSQREE
                     RLEKLLTRIKEMGLKEEDYSWYLDLRKFGGAPHAGFGLGFERMIMYLTGMENIRDVIP
                     FPRTPGNADF"
     gene            1905..2138
                     /locus_tag="P5056_03605"
     CDS             1905..2138
                     /locus_tag="P5056_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62826.1"
                     /translation="MKPRDNFTQEQADLLQEFKEKADKLGLEYFAVVGDPNDGTGASV
                     YSAKTPESAARNAREAHIEWEKRHSIDPEHSRA"
     gene            2237..2830
                     /locus_tag="P5056_03610"
     CDS             2237..2830
                     /locus_tag="P5056_03610"
                     /inference="COORDINATES: protein motif:HMM:NF021356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62827.1"
                     /translation="MVVEEILNILNGVAKDTSSIAEAMMCSRPESHKKFKLLNKYNQT
                     YWDDREKVDAKTPRKQNFYTTLNYLKNGGFIKKEKTKNGILWSITSAGVEKLNKIHKR
                     SLNSKSNIDYAKKIGDNLIIIAFDIPEKLSRNRYWLRGALKYFDFEMIQKSVWVGRCK
                     LPEQFIRDLKNREILNCVDIFEVGNKGTLHRLVNKAK"
     gene            complement(2956..3951)
                     /locus_tag="P5056_03615"
     CDS             complement(2956..3951)
                     /locus_tag="P5056_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62828.1"
                     /translation="MPSIQSHRGPNQRIFLFPNLNITPSDARDLLDYWGEGFGHSKVL
                     RPFDLDDGDDYLFGLKDAAIFCIFVPIGARFNEIMSAANGFVANNNYRLATRRELLEF
                     GKNLCFWEVDSLWGGLRNGSALAILYNPSYDYLYKSCSVFSEGYWARAGGLYYFLAID
                     KVSEDRERKLRIDGLSKDLCVVAAFSAGGCGTTRRQEGRITHEAYRLMDSDSYRCRDE
                     RTDTKKKVEVVIATAQEWTGFRDSKVKDVHAAGRKRGYDLCQPADAIRIHANYKGIKN
                     VVLAMWPLKVTGPFGFVFRILAGNGKPWLDVEECWANLDYPAGDDDLYAFRRLKT"
     gene            4431..>4911
                     /locus_tag="P5056_03620"
     CDS             4431..>4911
                     /locus_tag="P5056_03620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019976375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRY62829.1"
                     /translation="MTQKEALDILKMGHNAFITGAAGSGKTHLLNEYIKYLKDHDVEI
                     GITAATGIAASHIGGQTIHAWSGLGIKDSLSLYDIENLEEKPYLWKRFDKVKVLIIDE
                     ISMLHHFRLDLVEQILRSFKRNNLPFGGLQVIFCGDFFQLPPVSRMGEREALFAYHSD
                     "
BASE COUNT         1552 a         1014 c         1095 g         1250 t
ORIGIN      
        1 cggatgtcgc caagcgcttg cgtgtaaaat ttgaagagaa atattctgaa ttttccaaag
       61 aggccgcaaa acaaaatata aaatggacca cggttgtaag acttgcctct ataatccagc
      121 gagaagctgc cggcaaagaa gacatgccgc ttatctcggg tattttatgg aataggctag
      181 ataaaggagt gaagcttgag gttgatgcat ctattcaata tataagaggc gatgtcggca
      241 aaggattctg ggctccaata agcgtttctg acaagaaaat agattctcct tacaatacat
      301 atatgtataa agggcttcct cctcacccta tatgtaatcc cggtgcagag gccatcaagg
      361 cagttctcta tcccgaaaag accgcctgta tattctatct acacgacccg ctcgggaaga
      421 tacactgcgc aaaagattat agtgaacact taaaaaatat taaggagttt ctaaaataat
      481 tagggttgaa taaggcccaa aaaagtatat atttgtatga tgacaaataa agatatcttt
      541 agagaaggag agaagctaat cggctctaaa ataagcgtga acggctgggt aagaacagct
      601 aggtcatcag gcacattcgg atttatcgag ctgaacgacg gaaccttttt caaaaacctg
      661 caggttgttt ttgacgataa actttcaaat tttaaagaga tggaaaaggt aaggattggc
      721 tccgcccttc gtattgaggg agaggtggtc aaatcaccgg caaaaggaca ggcttttgaa
      781 ttaaaggccg agaagataga catggtggct gattctgatg aaagcttccc gctccaaaag
      841 aaaaggcaca cttttgaata cctcagaaca atcgcgcact tgagaccaag atcaaatact
      901 ttctctgctg ttttcagagt tagatcactc gcggcttttg ctattcataa attcttcaac
      961 gaaaaaggat ttgtatatgt tcacacgcca ataatcacgg gcagcgatgc ggagggggcg
     1021 ggagaaatgt tccatgtcac tacgatggac ctcaaaaatc cgacaaaaga cgacaagggc
     1081 aacatcgatt actcaaaaga ctttttcgga aaagaaacaa atctcaccgt aagcggacaa
     1141 ttagaagggg agacgttcgc gcttgcgttc aataaaattt acacattcgg accgactttc
     1201 agagctgaga actcaaatac aaagagacat gccgccgagt tttggatgat cgagccggaa
     1261 atagcttttg ccggtctaaa agaagacgct gagctcgctg aagagatgat gaaatatatc
     1321 atcaagtatg ttcttgataa tgctccggaa gaaatggagt ttttcaataa atttatagat
     1381 acgaccttaa tggataggct tcagaacgtc ctcaattcat cattccaaaa agtcacatac
     1441 acagaggccg taaaattact cgaggaatcg ggcgcaaagt tcgaataccc tgtaggctgg
     1501 ggcaaggagc ttcaaacaga gcacgaaaga tatttgaccg agacaatatt caagaagccg
     1561 gttatcgtta cagattatcc aaaagatttc aaagcttttt acatgaggca gaatgatgac
     1621 ggaaaaacgg tcgcggcgat ggatattctc gtccccggaa ttggtgaaat aatcggtggc
     1681 tcacagagag aagagaggct tgagaaactc cttacaagga ttaaggaaat gggccttaaa
     1741 gaagaggatt attcatggta tctggatcta cgaaagtttg gcggcgctcc tcacgcggga
     1801 tttggcctcg ggtttgagag aatgataatg tatttaaccg ggatggagaa catcagggat
     1861 gttataccgt tcccaagaac tccgggcaat gcagattttt aaaaatgaaa cctcgagata
     1921 attttacgca ggagcaggcc gatctgcttc aagaattcaa agaaaaagct gacaaacttg
     1981 gtctggaata ttttgccgtg gtcggggacc ccaatgacgg cacaggggcg tctgtgtatt
     2041 ccgcaaaaac gcctgaaagc gccgcaagaa acgctcgtga ggcgcatatt gaatgggaga
     2101 agaggcatag catcgaccct gaacacagcc gagcataacc gcaggcaacc attactacaa
     2161 tatcaccccg attgtgccag tattgtagta ataataaatc attcaaattt aattctataa
     2221 gataaacaag ataatcatgg tcgtcgagga aatacttaat atattaaacg gagtagcaaa
     2281 agacacctct tctattgcgg aagcaatgat gtgttcgagg ccggagtctc ataaaaaatt
     2341 taaactgctg aacaaataca atcaaactta ttgggatgat agagagaagg tggatgccaa
     2401 aactccgaga aagcagaatt tttataccac ccttaattat ctgaaaaatg gtggttttat
     2461 aaaaaaagag aaaacaaaga acggaatttt atggtcaata acttctgccg gcgtagaaaa
     2521 attaaataag attcacaaaa ggtctttaaa ttcaaaatcg aatatcgatt atgctaaaaa
     2581 aataggggac aatcttataa ttattgcctt cgatatcccg gagaaacttt ccagaaatcg
     2641 ctattggctt cgcggggctc taaaatattt tgattttgaa atgatccaaa aaagtgtgtg
     2701 ggtcggaaga tgtaaattac cagagcaatt tatacgggat ctaaaaaata gagaaatact
     2761 aaactgcgta gacatttttg aggtgggcaa caaggggact ctgcacagac tagtaaataa
     2821 ggctaaataa gtcaggcttc ctattactac aatgctggca caatcggggt gaggttgtag
     2881 tgagaaaaag acaaaagaaa aggcgctcta gttaagagcg ccaaaatatt ttagacccga
     2941 tgatttattt ggtcattaag ttttcagccg gcggaacgcg taaaggtcgt cgtctccggc
     3001 cggataatcc aaattggccc aacactcttc gacatctaac catggtttac cattcccggc
     3061 aagaatacga aaaacaaaac cgaatggtcc cgtcactttg aggggccaca tcgcaaggac
     3121 cacattcttg atgcccttat aattcgcatg aatacgaatg gcatcggcgg gttgacacaa
     3181 gtcataacct ctcttgcgcc cggcagcgtg gacatctttg accttagaat ccctaaagcc
     3241 agtccactcc tgtgccgtcg cgatgactac ctcaaccttc tttttggtat ccgttctttc
     3301 atcacgacag cgatagctgt cagagtccat caatctgtag gcctcatgag tgatcctgcc
     3361 ctcttgtctt cgcgttgtac cacaaccacc ggcactgaag gctgcaacga cacagagatc
     3421 tttagacaga ccgtcgattc ttaattttct ttctctgtct tcggacacct tgtcgatcgc
     3481 gaggaaataa taaaggcctc ccgctcttgc ccaatagccc tctgaaaaaa cagagcaact
     3541 cttataaaga taatcgtacg aaggattgta caaaatagcg agtgcggagc catttctaag
     3601 accgccccaa agcgagtcaa cctcccaaaa acaaagattc tttccgaact ccaacaactc
     3661 tcttctcgtt gcaagacggt aattgttatt cgcgacaaat ccattcgctg cgctcataat
     3721 ttcattaaat cttgcgccta tggggacaaa tatacaaaag attgcggcgt ccttcaaacc
     3781 aaagagatag tcgtctccgt catcgagatc gaatggccgc aatactttgc tgtggccaaa
     3841 tccctcaccc caatagtcga ggagatctct tgcgtcgctg ggcgtaatat taagattcgg
     3901 gaaaaggaag atgcgctgat tgggtccacg atgagattgt attgaaggca tacgagagcg
     3961 catgttcata tttgtccctc tttctggcca tttaaggccg tccacggttg ttctcgtttg
     4021 ttacccccaa gccccaacct ttcttgtatt ttcaatgttc ttttgaatcc acgggcacat
     4081 taccacatca ttttttttct tgtcaaatgg caaatggact acaacacgaa cattttttta
     4141 ttgtcaagtc aagggcacaa ataggcgcta gtaatctcaa catattccac cgttaccgct
     4201 aggtttttta ttaaatattt tctgatatag taaaagaatc gtcctgataa ataatggcgt
     4261 aacgagtcgt acaaaaaacg tagcgaggct cgaatacgtt ttcgaaggcg tatcacgata
     4321 cgccgagaaa tcgtaagaag agccgtagta gtgttttgga cgacgcagta gccaagcgac
     4381 ttttcgtcca atgtaataat agtgcttatt tatcagaacg attctttgct atgacacaaa
     4441 aggaggcgct cgacattctt aagatgggac ataatgcttt tataaccggc gcggccggga
     4501 gcggaaagac gcaccttctg aacgaatata taaaatatct aaaagaccac gacgtggaga
     4561 ttggcatcac tgccgccacg ggtatcgcgg cctcacacat tggagggcag actattcacg
     4621 cgtggtcggg tttgggtata aaagattcgc tgtcgctata tgacatagag aatctcgaag
     4681 aaaagccgta cctctggaaa agatttgata aggtaaaagt tcttattatt gatgagattt
     4741 ccatgttgca ccattttcgc ctagatctcg tggagcagat attgaggtca tttaaaagaa
     4801 acaatctgcc gtttggaggg ttgcaggtaa ttttctgcgg agacttcttc cagcttcccc
     4861 ctgtttctag aatgggagaa agagaggcgc tttttgctta tcactcggac a
//
LOCUS       DAOTEX010000057         4793 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3118
            NODE_12890_length_4793_cov_4.090756, whole genome shotgun sequence.
ACCESSION   DAOTEX010000057 DAOTEX010000000
VERSION     DAOTEX010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868142
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4793)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4793)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 883
            CDSs (total)                      :: 833
            Genes (coding)                    :: 829
            CDSs (with protein)               :: 829
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4793
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12890_length_4793_cov_4.090756"
                     /isolate="AMR_MDS_3118"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..572
                     /locus_tag="P5056_03625"
     CDS             <1..572
                     /locus_tag="P5056_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62830.1"
                     /translation="KALSYSQLTPMLTKAIQELAASTTELSTKLDTLIGNGTISSNGL
                     WTADAGGHIKLVTASVVDFQNQDLINIKNVLSSSGSWSIKEDEGTFVGTELCLDEICI
                     SKDQLNVILQNANIISSPTAGGQIETTTISTTSADEQSTTTSEFISSEPAPDAQTPIV
                     EPELVTPPEETTAAEPSPIEPTPTEPIAE"
     gene            634..1617
                     /gene="obgE"
                     /locus_tag="P5056_03630"
     CDS             634..1617
                     /gene="obgE"
                     /locus_tag="P5056_03630"
                     /inference="COORDINATES: protein motif:HMM:NF008956.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HRY62831.1"
                     /translation="MLVDEVRIMTKGGDGGRGALIFGEDQFSKRPAGADGGNGGSVYI
                     IASRDVADLSRFRYEKSFEAEKGGDAFRSMQGKDGADIFLKVPRGTIVYNITSGESTE
                     LLEEGMKVRAARGGTRGRGNAAFSTARKSEPGKRELGHPGYEAEVRLELQLIADIGLV
                     GLPNVGKSSLLNTITGAKSKVGNYNFTTLEPFLGALPGGNVMADIPGIIEGASEGKGL
                     GVKFLRHIKRTKVIAHCISAESENPAEDYKVIRGELEKYSDELAKKPEFLIITKIDAL
                     NSAELKKKEALIKKLNKNYALVSIIDDKTVKNLVDALNKFVKKSIKSVHSV"
     gene            1731..2075
                     /gene="metG"
                     /locus_tag="P5056_03635"
     CDS             1731..2075
                     /gene="metG"
                     /locus_tag="P5056_03635"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HRY62832.1"
                     /translation="MTTEKKPNISYDDFSKLDLRVGTIVAADDLENSNKLIKFMIDDG
                     SEKRQIIAGIKKQYKVEDLVGKQVVFVANLEPREIAGLVSNGMILAAHTLDNLPVVLF
                     PERSVPDGSEIC"
     gene            2085..2480
                     /locus_tag="P5056_03640"
     CDS             2085..2480
                     /locus_tag="P5056_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62833.1"
                     /translation="MKKLLKFFDKLEDHVREYLSGVPVIYSLIGATAIVVFWDGISRV
                     MDSIEIFDGFWGGMLAIFISVVVLLLTGLLVSFFVGDVIILSGIKHEKKSTDKTREEL
                     LEEEKELKDAIKKISHIEHDMEEIKQKIK"
     gene            complement(2491..2949)
                     /locus_tag="P5056_03645"
     CDS             complement(2491..2949)
                     /locus_tag="P5056_03645"
                     /inference="COORDINATES: protein motif:HMM:NF020977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HRY62834.1"
                     /translation="MPLHQDIKDGIKPAMIAKDAVKLGVLRGLIADFINELVTKKRRP
                     DELLSDEEAMAVIKRAAKRRMDSIEQFKGGGRNDLAESEEKELTVLKTFIPAGMSREE
                     IEKIALTKKEELQISEPAKAGLLMSHMMKELKGKADGNDVKFVVDKILNK"
     gene            3050..>4793
                     /locus_tag="P5056_03650"
     CDS             3050..>4793
                     /locus_tag="P5056_03650"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HRY62835.1"
                     /translation="MKDQSFNELYRKLNPRQKEAVDTIEGPVMVVAGPGTGKTQILGL
                     RIANILRKTDASPDSILALTFTDSGVHSMRKRLVSIMGSSAYRVNIFTFHGFCNNLIK
                     DHPEYFDRIISSRNISPLDQIKMMETIVDKAKLEVLRPFGDRFYYVESVLKSISDLKR
                     EAVSPEDFSKLLEKEEALYESASDLYHTKGAHKGKMRGVYIDIFNNLKKNKELLVLYK
                     GYEKALRKSALYDYDDMIMEVVAALKKNDELLLKLQENFQYILVDEHQDTNNAQNSVL
                     ELLAGFYESPNLFIVGDEKQAIFRFQGASLNNFLYFKELYKEAKIIKLEENYRSTQTI
                     LDSAHSLITKSVGPSELRSRLIARAGGKSNNIVLHSFSSDDSEILGVVKNIKDKINSG
                     VKPGDIAVIYRDNKDAFPLGRALERTQIPFVIESDQNILEDEDMKKLVTLMEAVNDFG
                     EEEKFLRCLHLDFLNIDEFFIYKIINYARKEKVSVLDMLSKDSLPREFPERDVSSLKD
                     IYNKISAWSKVSKNEGLLDFFETLVRDSGFLNYLLCGVDSVAKMEKLSVVFDNIKKIV
                     EDHREMRLHGFMDFL"
BASE COUNT         1477 a          841 c         1113 g         1362 t
ORIGIN      
        1 ttaaagctct ctcatactct cagctcacac ctatgctcac aaaagctatc caagaacttg
       61 ccgcttcaac gacagagctc tccacaaaac ttgatactct catcggaaac ggcaccattt
      121 cttcaaacgg cctctggact gcagatgcgg ggggacacat taaacttgtc accgcttcag
      181 ttgtagactt ccagaaccag gatctcatta acatcaagaa tgttctctct tcttcgggga
      241 gttggagtat aaaagaagat gagggaactt ttgttggtac ggaactttgt ttggatgaaa
      301 tatgtataag caaagatcag cttaatgtta tccttcaaaa cgccaacatt atatcttctc
      361 caaccgcggg agggcagata gagaccacaa ctatatccac cacttcagct gacgagcaaa
      421 gcacaacaac ttctgaattt atatcatcag aacctgcgcc ggacgctcaa acaccaatag
      481 tagaaccaga actggtaact cctcccgaag agacaacagc cgctgaacct tctccaatag
      541 agccaacccc tacggagcca atagctgaat aaatataacc ccgccacttc tggcggggtt
      601 ttaatttgag gctttttttg ctattctaaa tctatgttag ttgatgaagt tagaataatg
      661 acaaaaggcg gagacggggg gcgaggcgcc ctcattttcg gcgaggatca attttccaag
      721 aggccggccg gggccgacgg aggaaacggc gggtctgtgt atataatagc ctcgcgcgat
      781 gtagcggact tgtccagatt caggtatgaa aaaagttttg aggctgaaaa gggcggtgat
      841 gctttcagaa gtatgcaagg caaagatggc gccgatattt tcttgaaagt gccgagggga
      901 acaattgtct acaatataac aagcggagag tccacggagc ttttggagga ggggatgaag
      961 gttagggcgg caagaggcgg aacgagaggt agaggaaacg ccgctttttc taccgcaaga
     1021 aagtcagaac ctgggaaaag agaactcggg catccggggt atgaagcaga agtccgtctg
     1081 gagcttcagc ttatagccga tatcggtctc gtgggtctcc cgaatgtggg aaaatctagc
     1141 ctgctaaata caattacagg cgccaaaagc aaagttggga attataattt tacaacgctc
     1201 gaaccatttt tgggtgcctt gccgggagga aacgtgatgg ctgatattcc cgggattata
     1261 gagggggcgt cggaaggaaa ggggcttgga gtgaagtttc ttagacatat aaaaaggact
     1321 aaggttattg cacattgtat ttctgcggaa tcggagaatc ccgctgaaga ttataaggta
     1381 ataagagggg agctggaaaa atacagcgac gagcttgcaa agaagccgga gttcttgatt
     1441 attacaaaaa tagatgcgct gaactccgct gaattaaaga aaaaagaagc attaataaag
     1501 aaactgaata agaattatgc tttagtctct attattgatg ataagaccgt gaagaatttg
     1561 gttgatgctt tgaataagtt tgttaagaaa tcaattaaat cagttcactc tgtttagtga
     1621 tccaatcgag taaaatgacg caggtagtgt gtattgttat gtagcgataa ttattagtat
     1681 atacatagat agacgttaca aatctttact taacagggta aacaataaaa atgaccacag
     1741 aaaaaaaacc aaatataagt tatgatgatt tttctaaact agatcttagg gtcggaacta
     1801 ttgtggcggc tgacgatttg gaaaattcca ataagctaat taagtttatg attgatgacg
     1861 gctccgaaaa aagacaaata attgcgggta taaaaaagca gtataaagta gaggatttgg
     1921 tcgggaagca ggttgtcttt gtggcgaatc ttgagccacg ggagatcgcc ggtttggtgt
     1981 cgaacgggat gattttggcc gctcatactt tggataattt accggtagtt ttattccctg
     2041 agcgatcagt tcccgacggt agtgaaattt gttaatttgt aaatatgaaa aaactgctta
     2101 aattttttga taaattagaa gatcacgtca gagagtatct aagcggcgta ccggttattt
     2161 atagtttgat aggagccacc gcaatcgttg tattttggga cgggatatct agggtaatgg
     2221 attcaatcga gatatttgat ggtttttggg gagggatgtt ggctattttt atcagcgtcg
     2281 tcgtcttgct tttgacagga ttactagtgt cgttttttgt tggagacgtt atcattcttt
     2341 ccggaataaa gcatgaaaaa aaatctaccg ataagacaag ggaggagctt ttagaagaag
     2401 agaaagagct caaagatgct atcaagaaaa taagtcacat tgaacatgac atggaggaga
     2461 taaagcagaa aataaaataa ttttacggct ttatttgttt agtattttgt cgacaacaaa
     2521 ttttacatca tttccgtcag ccttgccctt tagctctttc atcatatggc tcattagaag
     2581 acccgctttg gccggttcac ttatttgaag ttcttctttt ttagttaatg caattttttc
     2641 tatttcttcg cgtgacatgc cggcggggat aaaggtttta agaacagtca attccttctc
     2701 ttcgctctcg gcaaggtcat ttcttcctcc gcccttgaat tgctcgattg agtccatcct
     2761 tctctttgcg gctcttttga taaccgccat cgcttcttcg tctgacaaca attcatccgg
     2821 tcgcctcttt ttggttacaa gttcgtttat gaaatcagca ataagtcccc ttaatactcc
     2881 cagttttacg gcgtcttttg ctatcatcgc cggcttgatg ccgtccttta tatcttgatg
     2941 taatggcata aaattagtta aaagttaaat gtttataaag tctataaagt taaagaagtg
     3001 taatcatttt aaatgacagc ctctttcggt tacgttagaa tagtatcaca tgaaggacca
     3061 atcgttcaac gaattatata gaaagctaaa cccaaggcaa aaagaggcgg tagacactat
     3121 cgaagggcct gtaatggtgg tagcgggtcc cggcaccgga aagacccaga tattaggttt
     3181 gcgtattgcg aatattttga gaaaaacaga cgcctcgcct gacagcattt tggctctcac
     3241 ttttaccgat tcgggggttc actcaatgag aaagaggctc gtgtctatca tgggaagttc
     3301 ggcatataga gtgaacattt ttaccttcca cggattttgt aacaatctta taaaagatca
     3361 tcctgaatat tttgacagaa taataagttc aagaaatatt tctccactcg atcaaataaa
     3421 aatgatggag acaatcgttg ataaagctaa acttgaggtt ttgcggccgt ttggagacag
     3481 gttttattat gttgaatctg ttttgaagtc gattagcgat ttgaaaaggg aagctgtttc
     3541 cccggaagat ttttccaagt tgctggaaaa agaagaagcg ctttacgaga gcgcctcgga
     3601 tctttatcac acaaaaggcg cgcataaggg caagatgcgg ggggtgtata tagatatatt
     3661 taataatctc aaaaagaaca aagaactcct ggttctttat aagggctacg aaaaagcatt
     3721 aaggaaaagt gcgctttacg attatgacga tatgataatg gaggttgtgg ccgcgcttaa
     3781 gaaaaatgat gaattattgc tcaaattaca agagaatttc caatatatat tggtagatga
     3841 gcaccaagac acaaataacg cgcagaacag cgttttagag cttctggcgg gcttttacga
     3901 gtcgcctaac ttatttattg taggtgatga aaaacaagcg atatttaggt ttcaaggggc
     3961 gtctttgaac aattttcttt attttaagga actttataag gaggccaaaa ttattaaatt
     4021 agaggaaaat tacaggtcta ctcaaacgat actagattcg gctcacagct taataacgaa
     4081 gagcgtcggt ccgagcgagt tgcgaagtcg gcttatagca agggcgggag gaaaatcaaa
     4141 taatatagtt ctgcatagtt tttcttctga tgattcggag atactcggcg ttgtaaaaaa
     4201 tataaaagat aaaataaatt ccggagtgaa gccgggggat atcgccgtca tttatagaga
     4261 caacaaggac gctttccctt tggggcgtgc gcttgagaga acgcagatac cttttgttat
     4321 agagtcggat caaaacattc ttgaggacga agatatgaag aagctggtga ccctcatgga
     4381 ggctgttaat gattttggcg aagaagaaaa atttttaaga tgtttgcatc ttgatttctt
     4441 aaatatagat gaatttttta tttataagat cataaattac gctagaaaag aaaaggtgtc
     4501 ggttttagat atgctgtcga aggattctct gccgcgcgaa ttcccggaac gagatgtctc
     4561 ttcgttaaaa gatatttata ataaaatttc cgcttggagc aaggtgtcaa aaaatgaagg
     4621 gcttttggat ttttttgaga cgctcgtaag agattccgga tttttaaatt atttgctttg
     4681 cggtgttgac tcggtcgcaa aaatggaaaa gctctctgtt gtctttgata acataaaaaa
     4741 gatagtagaa gaccacagag aaatgagact ccacggattt atggatttct tgg
//
LOCUS       DAOTEX010000058         4783 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3118
            NODE_12933_length_4783_cov_5.224619, whole genome shotgun sequence.
ACCESSION   DAOTEX010000058 DAOTEX010000000
VERSION     DAOTEX010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868142
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 883
            CDSs (total)                      :: 833
            Genes (coding)                    :: 829
            CDSs (with protein)               :: 829
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4783
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12933_length_4783_cov_5.224619"
                     /isolate="AMR_MDS_3118"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2279)
                     /locus_tag="P5056_03655"
     CDS             complement(<1..2279)
                     /locus_tag="P5056_03655"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HRY62836.1"
                     /translation="MNLRRIIILSLLFFPAAKIFAYSPDTTHKGLAEQSVIFYNRNFS
                     RQVSDPEKELMITGAASEDSPVARVLNHFYDPIRNIGINNYTNAKDWATKDGTGNVYT
                     WSAAINEYAKGNKENAFIYLGHILHLLEDMSVPDHTRNDPHMGVLNTGESSYENWAKL
                     SKDRATLKGVVDNYSNEKPLYFNNILEYFDFVANYSNKNFFGDDSIKNDVYQYQYPKI
                     YKIDGFYGYGRDEIGGDSFRLVKLINNPKNLGEKMVILSDKNDTSVLSGYFDRLGKQA
                     ILAGAGVIDLFLREAETARAEYLKRQVEIAKIETQKAIDLNAKLQNSNFAELVVYGAS
                     SFIGDKIVSPISSTIVSATDNFSSGSRVVSTTVRSAASFASFTTTSLAKQAAASVGSV
                     FASPAKTLETVTAIPAKNNIPDISLAISNTADISTEVSPINSQVANEPAESVQPPLPE
                     DYFSKLSEAYSALSLLNDLTPKQAPVTVSLPAPYSPGFGAGGDAQPPVDNVIEPEKEP
                     ISGTPTESAPSPEPASMPEEEAEASSTESTLIEEIIEAVLPILDITAPDVSLTVLSCD
                     DSLADDGCLLATTTVSVSWSSGAEDLDYFRVTRDGETSTTTDTSLIFITENKTSHKIS
                     VEAVDKTGNFSDSVSKTFKIFKNPIIINEVAWGGTRGHSEDEWIELRNLSDETISIDG
                     WKLLSKTDNSPNITLSGKISPNGYYLIERKNDEETDEAAESPVRNVPADLWVSFGTGL
                     NNGGENLVLSRASTTLDEVP"
     gene            complement(2279..2914)
                     /locus_tag="P5056_03660"
     CDS             complement(2279..2914)
                     /locus_tag="P5056_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62837.1"
                     /translation="MISEANKDGKEMCPNGNKWSWLARIIGKILAILFALFVGIAALG
                     YYWPTIQEYRYKMEAERFMNEINAEKARILALEKADTFGGKTPEETFDMFLNALKAGD
                     TELASKYYDVTVQSEVLEGLKKELSEKGNLDLSIKYFTDVRGGEKKCSDIETDDGGCV
                     FEYIYTTDKDETVSISGRSETFFSPKGSQGVKAVDFKLIAFSKIWKITQPF"
     gene            complement(3006..3626)
                     /locus_tag="P5056_03665"
     CDS             complement(3006..3626)
                     /locus_tag="P5056_03665"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004068095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HRY62838.1"
                     /translation="MKAREKQLLLENMKAELSALKRSPLYRYRVENNYLPVVGEGSVS
                     ANVMFIGEAPGRNEAKTGRPFCGSAGKILDELLDSIKMERGDVYVTNIVKDRPPKNRD
                     PLPEEIKVYGPFLDKQIELIQPKVIATLGRYSMKYILEKFGLHGELRPIGEMHGKFLE
                     ADAPYGKIKIAILYHPCVAIYNRKKLPGLKKDMKIINKTCPPKFSQ"
     gene            complement(3628..>4783)
                     /locus_tag="P5056_03670"
     CDS             complement(3628..>4783)
                     /locus_tag="P5056_03670"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HRY62839.1"
                     /translation="DGTILDSLDALSGWPAGDSATKQTMQWSGGAWITATATPKAANV
                     KLTVGDSSENTNSSATTTAESATQVNYSYSSHSGSSELSREAKNIKAYLGAGRDRVVL
                     TGTPVQFSAKFTDESGSVLYGDNYKWSFGDAGYSQGEKTAHIYDIAGEYVVVLSATFG
                     GQEYTSRANIKAVPPELKVEAIVKNNGGYVVYVTNNGANEVNLKGWQLRTRRRFFILD
                     ADIIVMPKKTIPLSDKYTNMDLSAGEEMELLYPTGKIFEVIKAGKESDVESIKRDKEC
                     SPRSELCSATGVDKGVDNPVDNVGTELWKKELLSLEEQLRGVQNSLSANISTLKRVPV
                     SVENEEKIKDDRAVVEQVATTTDNVEKIVISPQKRESTLWKFLRGIFGRK"
BASE COUNT         1216 a         1196 c          889 g         1482 t
ORIGIN      
        1 ggcacctcgt ccaatgtggt agacgcccga gacaaaacta aattctctcc tccgttgtta
       61 agccccgtgc cgaagctgac ccaaagatct gccggcacat ttctaacggg gctctctgcg
      121 gcctcgtccg tctcttcgtc attttttctc tctataagat aatatccgtt tggtgaaatt
      181 tttcccgata aagttatgtt cggagaattg tcagttttag aaagtaattt ccagccgtca
      241 atacttatag tttcgtcaga cagatttcga agctctatcc attcatcttc agaatgaccc
      301 cgcgtccctc cccaagcaac ctcatttata attatcgggt ttttaaatat tttgaaagtc
      361 tttgagacgg aatcagaaaa atttccggtt ttatctaccg cctcgacaga tatcttgtga
      421 gaagttttgt tctcggtaat aaaaattagc gaggtgtcgg ttgtggtgct cgtctccccg
      481 tccctagtca ccctaaaata gtccagatct tctgcaccgg aactccaaga cacagatact
      541 gtagtcgtgg ccaaaaggca accatcgtcc gcgagagaat catcgcaaga tagaaccgtc
      601 aaagaaacat ccggagccgt aatatcgagt atcggcaaaa cagcctctat tatttcctca
      661 attaaggtcg attcggtgct ggaggcctcg gcttcttcct caggcatgct tgcaggctcc
      721 ggcgaaggtg ccgattctgt cggtgtcccg gatattggct ctttttcagg ctctattacg
      781 ttatccacag ggggctgagc gtctcctcca gctccaaaac caggcgaata tggagcgggt
      841 agagaaactg tcacaggagc ctgttttggt gtcaaatcat taagcaaaga aagtgcggaa
      901 tatgcctcag aaagttttga aaaataatct tcagggaggg gaggttgcac gctttccgcc
      961 ggctcattag ccacttgcga attaatagga gaaacctctg tggaaatatc agctgtattg
     1021 cttatcgcca aagaaatatc cggaatatta tttttagcgg gtatcgccgt aactgtttct
     1081 aacgtttttg ccggtgaagc aaagacgcta cccacactcg ccgccgcctg cttggcaaga
     1141 gatgtcgtcg taaacgaagc gaaggacgcc gcgcttctaa ctgtagtgct aaccaccctc
     1201 gaaccggatg agaaattatc ggttgcggaa acaatggttg agctaattgg agaaacaatc
     1261 ttgtcgccga taaacgagga cgcaccgtaa acaacaagtt ccgcaaaatt tgaattttgt
     1321 aattttgcat taaggtcaat tgctttttgc gtctctattt ttgcaatttc gacctgtctc
     1381 ttgagatatt ccgcacgagc cgtttcggcc tcacgtaaaa agaggtcaat cacacctgcc
     1441 ccggcgagaa tagcctgttt cccaagacgg tcgaaatagc cggagaggac agaggtgtca
     1501 tttttatcgg agagaataac cattttttct cctaaatttt ttgggttatt gatcaatttt
     1561 accagtcgaa aagaatcgcc acctatttcg tctcgaccat atccatagaa accatcaatc
     1621 ttataaattt ttggatactg atattgataa acatcgtttt ttattgaatc atctccaaaa
     1681 aaatttttgt tagaataatt agccacaaaa tcaaaatatt ccaaaatatt attaaaatat
     1741 aacggttttt cgttgctata gttatccaca acccccttga gggtggctcg atccttacta
     1801 agttttgccc aattttcata agagctctca cccgtattca aaacccccat atgagggtcg
     1861 ttcctcgtat ggtccggcac gctcatatcc tctaagaggt gcaaaatgtg ccccaaataa
     1921 ataaaagcgt tctctttatt gccttttgca tattcattta tagccgcact ccacgtataa
     1981 acgttaccgg ttccatcctt cgtggcccaa tctttggcgt ttgtataatt atttataccg
     2041 atatttctta tcggatcata gaaatgattc aaaaccctcg cgacgggact atcttccgat
     2101 gccgctccgg ttatcatcaa ttctttttca ggatcactga cctgccttga aaaatttctg
     2161 ttataaaaaa tcaccgactg ctccgcaagc cccttgtgtg tagtatccgg gctgtatgcg
     2221 aaaattttcg ccgcaggaaa aaacagtaga gataaaatta ttattctcct taagttcatt
     2281 agaatggttg tgttattttc cagattttag aaaaagctat caatttaaaa tcaacggcct
     2341 ttaccccctg acttcctttc ggcgaaaaaa aagtctcact cctcccagag atggaaactg
     2401 tttcatcttt gtcggttgta tatatatatt caaaaacgca tccgccatca tccgtttcta
     2461 tatcactgca cttcttctcc cctcctctca catccgtgaa atattttatg ctcagatcca
     2521 ggtttccttt ttccgacaat tctttcttta gcccctccag cacctcgctc tgcactgtca
     2581 catcataata cttgctggcc agctcagtat ctccggcctt aagggcgttt aagaacatat
     2641 caaacgtctc ctccggtgtc tttcctccga atgtgtcggc cttttctagg gccaatattc
     2701 tagctttttc ggcgtttatc tcattcataa acctttcagc ctccatcttg tatctatatt
     2761 cctggatggt cggccagtag tatccgagag cggcgattcc cacgaacaag gcaaacaaaa
     2821 tagccaaaat cttgccgata attctcgcaa gccaagacca tttattcccg tttggacaca
     2881 tttcttttcc gtctttattc gcttcagaga tcatttcttt attttattta ccctcctaaa
     2941 ttttttataa ttataacatt tatttggaca ttgtgcgctg gtcgccttca attgtttttt
     3001 atattttatt gtgaaaattt tggtgggcaa gttttattga taattttcat atcttttttc
     3061 agacccggta gcttcttcct attatagata gcgacacaag ggtgatacag gattgcaatc
     3121 tttatttttc catatggcgc gtctgcttcc aaaaactttc cgtgcatttc acctatcggc
     3181 cgaagctcgc cgtgtagccc aaacttctcc aaaatatatt tcatcgaata tcttccgagg
     3241 gttgctatca ctttaggctg tattaattct atttgtttgt ccaagaacgg cccgtaaact
     3301 tttatctcct ccggaagagg gtccctgttc tttggcggcc tatctttcac gatgtttgta
     3361 acataaacat cccctctctc catttttata gagtcaagaa gctcgtccaa tatcttcccg
     3421 gcagatccgc aaaaaggtcg cccggtcttc gcctcatttc tcccgggagc ttctccgata
     3481 aacatcacgt tcgcagaaac actcccctcg cccacgaccg gcaagtaatt attttccact
     3541 ctatatctat ataagggaga tctctttagc gccgacagct cggccttcat attttccaat
     3601 aacaattgtt tctcccttgc cttcatatta ttttcgccca aatatccctc ttagaaattt
     3661 ccacagggtc gattcacgct tttgcggaga aataacgatt ttttcgacgt tatcggtggt
     3721 ggtggcaact tgttccacaa ccgccctatc gtcttttatt ttttcttcat tttcaacact
     3781 tacaggcact ctctttagtg ttgatatgtt ggcagacaat gagttttgaa cacccctaag
     3841 ctgttcctct aaagacaaga gctccttttt ccacagctct gtccccacgt tgtccacagg
     3901 gttatccaca cctttatcca ccccagtagc agagcaaagc tcgctacggg gcgagcattc
     3961 cttatctctc tttattgact caacatcaga ctcctttccg gccttaatca cttcaaaaat
     4021 tttgcctgtc ggatataaga gctccatttc ttcccccgcc gacaagtcca tattagtata
     4081 tttgtccgaa agaggtatgg tcttttttgg cataacaata atatcggcat ccaaaataaa
     4141 gaaacgcctt cttgttctca gttgccaacc ctttaaattg acctcatttg caccgttgtt
     4201 ggtaacatag acaacataac cgccgttatt tttaacaatc gcctccactt ttaattccgg
     4261 tggaacagct tttatattcg cgcggcttgt atattcctgc ccgccgaatg ttgcgctaag
     4321 aaccacaaca tactcacccg cgatatcata tatatgagcg gttttttctc cctgagaata
     4381 tccggcatct ccaaaagacc acttataatt gtctccataa agcactgatc ctgactcatc
     4441 tgtaaattta gccgaaaact ggacaggcgt gccggtcaac accaccctgt ccctccccgc
     4501 tccaagatat gcttttatat tttttgcctc tctgctgagc tcagatgatc ctgagtgcga
     4561 agaataacta taattaacct gtgtcgcgga ctctgccgtt gtggtagcgc tcgaatttgt
     4621 gttttccgaa gaatcgccga ctgttaattt cacattggcc gcctttggcg tagctgtcgc
     4681 cgttatccat gctcccccgg accactgcat tgtctgttta gtagcagagt ctcctgccgg
     4741 ccagccggaa agagcgtcta gggaatcgag tattgtacca tca
//
LOCUS       DAOTEX010000059         4627 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3118
            NODE_13526_length_4627_cov_4.096675, whole genome shotgun sequence.
ACCESSION   DAOTEX010000059 DAOTEX010000000
VERSION     DAOTEX010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868142
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 883
            CDSs (total)                      :: 833
            Genes (coding)                    :: 829
            CDSs (with protein)               :: 829
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4627
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13526_length_4627_cov_4.096675"
                     /isolate="AMR_MDS_3118"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..909
                     /locus_tag="P5056_03675"
     CDS             <1..909
                     /locus_tag="P5056_03675"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRY62840.1"
                     /translation="EKSESFDWWGAITLGISLSSLVLVLDKGNEWGWFQLESLLCYAA
                     LLISLALFIVIEHREKEPIVDLKFFSNSVFVNTILNNVVVFMGMIGGVFIIPVFAQTF
                     LGLTATQSGYLFMPMAFMMMVASPIGGLFSGKIEPRFIISVSTLIASFGIFLFTYLDP
                     RSGALDIIIPLSIMAFGMGFGMAQRTSIITSVVPESEIGMASSVLALARNIAGAFGIA
                     VFGMILNTSIENNLLSFSRGVKINGMDPNLYQNAVSLLVVKSQIAAYVSVFKISAAIL
                     LVGAGVALLMKIPKNKIQDKEVFVLD"
     gene            1052..1351
                     /locus_tag="P5056_03680"
     CDS             1052..1351
                     /locus_tag="P5056_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62841.1"
                     /translation="MFDKYQKLISENPEGYWFRRKLYGWGWTPVKWQGWAVTFGYVAI
                     LVALGLSVDSSSSNKEVVDLFILPFIILTALFVRILYKKGESPRWQWGDNNKNEE"
     gene            1341..1796
                     /locus_tag="P5056_03685"
     CDS             1341..1796
                     /locus_tag="P5056_03685"
                     /inference="COORDINATES: protein motif:HMM:NF014678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HRY62842.1"
                     /translation="MRNRKLIFLAFILVVLFLLFYYSDLGVGQCGGYERKQIVIANTT
                     ISAEIADNECKRALGLSGRMSLDGDRGMLFKYEGEGSRGIWMKDMNFPIDILWLDKDL
                     NIVGMEWSVSQNTYPKIFGEEYVAEYVLELPVGFIAEHGVATGGSILIK"
     gene            1904..2326
                     /locus_tag="P5056_03690"
     CDS             1904..2326
                     /locus_tag="P5056_03690"
                     /inference="COORDINATES: protein motif:HMM:TIGR04272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62843.1"
                     /translation="MARFDQGGGKRFGGNTGGSRFGGKSFGRPSFGRKSFGDRGDREG
                     DRRGFEMHDAICSECGKNCQIPFRPTGEKPVYCSECFGAKGDRREDRRERRSFGDRRL
                     ARNNFSGNDDLKRQIEGMNAKLDKLAEAIETLVLRWEK"
     gene            complement(2391..2747)
                     /locus_tag="P5056_03695"
     CDS             complement(2391..2747)
                     /locus_tag="P5056_03695"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HRY62844.1"
                     /translation="MLKRQNFSSSLRCALNGFWVAYKEEPNFKTEIFFAILIPYLGYH
                     FGISTVEFLIITLTIGFVLATELLNTALEEICDKFHPEHDPRIEKIKDLAAAAVLVSS
                     VSAVIVGLIILGPYTY"
     gene            2852..3112
                     /locus_tag="P5056_03700"
     CDS             2852..3112
                     /locus_tag="P5056_03700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006644531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HRY62845.1"
                     /translation="MTKKDLIIFVVVLIAGVYYWMNSDGIVAKKEATEIANPAAVYCV
                     EQGGKSEIKTNPDGSQYGICITKDKQECDEWEYYRGGCVRNQ"
     gene            3114..3416
                     /locus_tag="P5056_03705"
     CDS             3114..3416
                     /locus_tag="P5056_03705"
                     /inference="COORDINATES: protein motif:HMM:NF013869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MscL family protein"
                     /protein_id="HRY62846.1"
                     /translation="MKGFIDFIREQGVVGLAVGFMLGGAVSKVVTALITDIINPFLGI
                     ALGVVGDLKSASISIGAVNILYGDFISVLVDFVVVALVVYLGVKGLGLDRLDKKKQ"
     gene            3722..3919
                     /locus_tag="P5056_03710"
     CDS             3722..3919
                     /locus_tag="P5056_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HRY62847.1"
                     /translation="MTGKIKTLLDKGFGFIAREGEAKDLFFHSKELNGVAFDDLRVGD
                     TLSFEVVDGPKGPSAVNVTRV"
     gene            complement(4026..>4627)
                     /locus_tag="P5056_03715"
     CDS             complement(4026..>4627)
                     /locus_tag="P5056_03715"
                     /inference="COORDINATES: protein motif:HMM:NF016202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HRY62848.1"
                     /translation="GEEFSTVFSLGTTTEEEGFVKEFVIKEDKSVKELGGGLCQVATT
                     MFRLALDAGLPITERTNHRYVVGYYGAGLDATIYDPHPDLRFKNDTGKYLLLQGTVKD
                     NQLTFEFFGQKDGRQINISEPILSDEKPIPDTKYLMSTDVPYATMQCSETPRKGITAE
                     VTYNVTYKEGKINEQKFKSVYQPWRKICLIGLATTTPNK"
BASE COUNT         1366 a          762 c         1099 g         1400 t
ORIGIN      
        1 gagaaaagcg aaagttttga ttggtggggt gccataacgc ttggaatttc cttatcttct
       61 cttgttttag tactcgataa gggcaatgaa tgggggtggt ttcaactgga aagcctcttg
      121 tgttatgcgg cattgcttat ttcactagca ctatttatcg ttattgaaca tcgcgaaaaa
      181 gagccgatcg ttgatttaaa attcttttca aactctgtct ttgtaaacac cattttgaat
      241 aatgtcgtag tgtttatggg gatgataggc ggagttttta tcataccggt ttttgcgcaa
      301 acattcctcg gactcactgc gactcaatcg ggatacctct ttatgccgat ggcattcatg
      361 atgatggtag cctctccaat cggcggttta ttctcgggga aaattgagcc gagatttatc
      421 atctcagtta gtacgcttat tgcctcgttc ggtatttttc ttttcacata tcttgacccg
      481 cgatcgggag ctcttgatat aattattccg ctttcgataa tggcattcgg tatgggtttt
      541 ggtatggcac agaggacaag tattattacc tcggttgttc cggaaagtga aatagggatg
      601 gcttcgtctg tcctggctct cgccagaaat atcgccggtg cttttggtat tgcggttttt
      661 ggtatgatac tcaacacgtc tatcgagaac aatcttctta gtttttcaag aggggttaaa
      721 ataaacggca tggatccaaa tctttatcag aatgcggtat ctcttttggt tgttaaatcg
      781 caaatagccg cttacgtgtc ggtatttaaa atatcagccg cgatacttct tgttggtgcc
      841 ggagtcgcgc ttttgatgaa aattcccaaa aacaaaatac aagacaagga agtttttgtc
      901 ttagattaaa aaaagattac acttggcccc gaccacggaa gagaacggtt ttttctaact
      961 acgtagaaca ataaaccggc tattttattt gttttttgtt ataataacat tgtggagata
     1021 gataaatatt taggagagta gaaataaaaa tatgtttgat aaatatcaaa aacttataag
     1081 tgaaaatcct gaaggttatt ggtttaggcg aaagctatac ggctggggtt ggactccggt
     1141 caagtggcag ggatgggcgg taacttttgg atatgttgcc attttggtgg ccctcggatt
     1201 atccgtggac agtagctctt ccaataagga ggttgttgat ctctttatcc tcccgtttat
     1261 tattttaact gcgttattcg tgcgaatatt atataaaaag ggggagtcgc ctcgttggca
     1321 atggggagat aataataaaa atgaggaata gaaaattaat cttcctcgct tttattttag
     1381 tggtgttatt tttacttttt tattatagcg atttgggcgt ggggcagtgc ggaggatacg
     1441 agagaaagca aatcgtaatc gcgaatacca caataagcgc agagattgcc gataatgagt
     1501 gcaaacgagc gctcggcctt tcgggcagga tgtctttaga tggcgacagg ggcatgctct
     1561 ttaagtatga gggggagggt tctcgcggaa tatggatgaa agatatgaat ttcccaatcg
     1621 atatattgtg gttagacaaa gatctaaata ttgtcggaat ggagtggtct gtgtcgcaga
     1681 acacctatcc aaaaatattc ggggaagaat atgtggccga atatgtgctg gagttgccgg
     1741 ttggcttcat cgcagagcac ggagtggcta ctggtggtag tattttaata aaataatcga
     1801 ctcattaaag gtgtcggacg gggtccatgg agagggcttg caattatcat taaaataatg
     1861 cagaatattg cgattgttcg tatttaataa gtaatttaaa caaatggcaa gatttgatca
     1921 gggaggcgga aagagattcg gcggcaacac gggtggctcc agatttggag ggaaaagctt
     1981 cggaagaccg agctttggga gaaagagctt tggtgatagg ggcgacagag aaggagacag
     2041 aagaggattt gaaatgcacg atgcgatttg ctccgagtgc ggaaagaatt gccaaattcc
     2101 ttttagacca acaggagaga agcccgtata ttgcagtgaa tgttttggcg caaaaggcga
     2161 cagaagggaa gacaggcgag agagaagaag ctttggagac agaagactag ctagaaacaa
     2221 tttctccggc aacgatgacc taaagagaca aatagaggga atgaatgcca aactagataa
     2281 gttggcagaa gcaattgaga ccctcgttct tcgctgggaa aaatagacag aaggtagagg
     2341 atagaaaata gttacataag attttaaaaa tcacccgttt gggtgatttt ttagtatgtg
     2401 tatggaccaa gaataataag tccaactatg accgcggata ctgaagaaac gagaacagct
     2461 gcggccgcca ggtcttttat tttttctata cgaggatcgt gttccgggtg gaatttatcg
     2521 cagatctctt cgagggcggt gttcaataat tcggtggcga gcacaaatcc tatggttagc
     2581 gtaatgatca aaaactctac ggtcgatatc ccaaaatgat aaccaagata ggggattaga
     2641 atcgcaaaga atatttctgt cttaaaatta ggttcttctt tataagcgac ccaaaatccg
     2701 ttgagggcgc atcttaaact gcttgaaaaa ttttgtcttt ttaacataaa ataattatat
     2761 aattttgttg tgtatattaa aaattggctt gagaattatt tttataatat aattaaacat
     2821 tatttagcag tttagtaagt aaagaaacaa gatgacaaag aaagatctaa taatatttgt
     2881 agtagtttta atcgccggtg tttattattg gatgaatagc gatggcatag tggcaaagaa
     2941 agaagccacg gagattgcaa accctgccgc agtatactgt gtagaacagg gagggaaatc
     3001 agaaattaag acaaacccgg atggaagcca gtatggcatc tgtattacaa aagataagca
     3061 ggagtgcgat gagtgggaat attacagggg cggatgcgtt agaaatcaat aatatgaaag
     3121 gatttataga ttttataaga gaacaggggg tagtcggatt agctgtaggt tttatgctgg
     3181 gtggtgccgt atcaaaagtc gtcactgcgc tcataacgga cataataaat ccatttctgg
     3241 gtattgcgct cggtgtagta ggggatctga agtccgcatc aattagcatc ggggctgtaa
     3301 atattctata tggtgatttt attagtgtgc ttgtcgattt tgttgttgtc gctcttgtgg
     3361 tatatctcgg agtaaagggg ttggggttgg ataggcttga taagaaaaag caatagttaa
     3421 aaatcggtcg aggcggctta gtttatggga cttaaggatt aaatataatt ttatggcaaa
     3481 aggaaatagt tcacacaaaa aagacgtaaa gaagcctaaa aaggctaaaa aataaggagg
     3541 ttatttttta agcgaataag tttttccggg tttccgttta acccttattg tcagccgtat
     3601 aaaaagatac ggatgttaat acttgacaaa attatatatt tagtatatac ttataatagt
     3661 gtaattaaca caggggaaca aagtcgaagt tttctaagct agcaaaatca taaatatcaa
     3721 aatgacaggt aaaataaaaa cacttttgga taagggattt ggattcatcg ccagagaagg
     3781 cgaagcaaag gatcttttct tccactcaaa agaattgaac ggtgtcgctt ttgacgactt
     3841 gagagttgga gatactcttt ctttcgaggt cgtagatggt cccaagggtc cgtcagccgt
     3901 aaacgtaaca agagtttaat ctttttacaa atagaaaatc cgcttcagaa atgaggcgga
     3961 tttttttatt taccctgttt agtaaaaatt taatcacgat taaattttaa atttttttta
     4021 aaattttact tattcggggt tgtggttgcg aggcctatca ggcagatctt tctccacggc
     4081 tggtatacgc tcttaaattt ttgttcatta attttgcctt ccttataagt cacattgtat
     4141 gtgacttccg cggtaattcc tttccttggg gtttcactgc actgcattgt cgcgtaagga
     4201 acgtctgtgc tcatcaaata ttttgtatcc ggtatcggtt tttcatcgga caaaataggc
     4261 tcactaatat ttatctgcct gccgtctttt tggccgaaga attcgaatgt aagctgattg
     4321 tcttttacgg ttccttgaag caatagatac ttacctgtgt cgttcttaaa acgtaggtcc
     4381 ggatgagggt cgtaaatggt cgcgtcaagc ccggctccat aatatccgac tacatatctg
     4441 tgattggttc gttctgttat cggcaaacca gcgtcgagcg ccagtctaaa catcgttgta
     4501 gccacctggc acaatccgcc cccaagctct ttcacgcttt tgtcttcttt tatgacgaat
     4561 tctttaacaa atccctcttc ttcggtggtc gtgcccaaag aaaagactgt cgaaaattct
     4621 tctcccg
//
LOCUS       DAOTEX010000060         4356 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3118
            NODE_14766_length_4356_cov_5.239014, whole genome shotgun sequence.
ACCESSION   DAOTEX010000060 DAOTEX010000000
VERSION     DAOTEX010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868142
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 883
            CDSs (total)                      :: 833
            Genes (coding)                    :: 829
            CDSs (with protein)               :: 829
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4356
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14766_length_4356_cov_5.239014"
                     /isolate="AMR_MDS_3118"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1253
                     /locus_tag="P5056_03720"
     CDS             <1..1253
                     /locus_tag="P5056_03720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62849.1"
                     /translation="MNKIKADGEDLKVYSNALDAEKSKVIADSAQKVCAKMLPQLEAA
                     KTEISRNDIVILQNNLDKCSGKTTEECKMINQLAGKFNEFNGAAKKFSSEIDTARNFC
                     SNPSEASFEKIAESLQGIKEDGEDLRILGKELQADQAEKASEKALCRAIVPLFATGKS
                     ELAAGLAKVKATQDSCSGSKDEKCASIRALAPKFSEVSTKGNELLTGMTSVETTCKTP
                     SDKAPSADFIKKIEGIRNSKDAVLKLVEALRAEQDKAATSGKGIRVEAEEEVSTNLLP
                     KTESWHSRKELNPSWRPPFFGAGVWYLSRGGESLSYNVNVPTAGDYSLWVRDYVDNFQ
                     PKGVRRVTYTINGKSLGTYPEVALAGSKIDAGKGAFGWHKVFVTKLNAGPNMMKVTKE
                     STTSGAAILDAFYFTTGSDVPPEK"
     gene            1264..1899
                     /locus_tag="P5056_03725"
     CDS             1264..1899
                     /locus_tag="P5056_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62850.1"
                     /translation="MNQELRIMNKKMTLNKKNNQGIAPLIIILIVALLAGGGGYTYLK
                     SKNISLPSLGLGSSTAKNLDEMTIDSGNMDFSLSTIGALDVAPLSLEIPNLSAPAIEI
                     PKTSVDIGTFNTSNINIGMPDLSSVMPAVSIPSGIPANIPIPSAPTSIPTSIPTDIPP
                     SAPTGASGAPAGMPTGAGAPSVDCSAFASVPSCSIITGPGGEMCKQCFPNK"
     gene            complement(2083..2508)
                     /locus_tag="P5056_03730"
     CDS             complement(2083..2508)
                     /locus_tag="P5056_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62851.1"
                     /translation="MSEFDKAKVSDQDLLAMMNKLLPSDEYKELKEEVCWCFVRYLRR
                     GDSYARIFSELDAKRILNRVVRKHEDFKFSGSAEDLCVLLEVTMHIFFAGKNVQNYLW
                     KRAEIIHRLSVEKGVSFQDIVNDESLAEGINFLIGKSEK"
     gene            complement(2518..3108)
                     /locus_tag="P5056_03735"
     CDS             complement(2518..3108)
                     /locus_tag="P5056_03735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62852.1"
                     /translation="MMPKEKIKKAVFRWSVLFALVHFALLIYFYGMNVPKDLVSFPMD
                     GKDAVPLFEFTRWFDPLAWPIFGAFFLLVFLPINEWTETEGDKYNRPIVNLCFSDILK
                     SGVFSFALSLIAGFILFGLLLSVIKGIAFGLLNGVLAGILISGYCTLLTGAILLVIWA
                     GTRALAVFERLRIDISFVERCLRPFEKLLNFLNAEE"
     gene            complement(3130..3303)
                     /locus_tag="P5056_03740"
     CDS             complement(3130..3303)
                     /locus_tag="P5056_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62853.1"
                     /translation="MTLVEILLHFFAFGFWGDPFYHFGIFLRVLANFSMFILFSDWMN
                     LSSLENEDAKKKE"
     gene            3637..4077
                     /locus_tag="P5056_03745"
     CDS             3637..4077
                     /locus_tag="P5056_03745"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HRY62854.1"
                     /translation="MINTIITNTFVGVLLFASSTSLTPTDLSKMIESSSAKQEVEIKC
                     DTKEKECIIALIEKYSKEFDYSPKMAKAIAYCESEYKETAKGDKGKAYGIYQFHKPTF
                     EMFARQKGEKLDYYDTEDNIKLAIWALANDKEHHWSCYKKVATR"
BASE COUNT         1442 a         1011 c          943 g          960 t
ORIGIN      
        1 tcatgaataa gatcaaagcc gacggtgaag atctcaaggt atacagcaac gcgctcgatg
       61 ccgagaaatc aaaagttatc gccgactctg ctcaaaaagt atgtgcgaaa atgcttcctc
      121 agctagaagc cgccaaaaca gaaataagca gaaacgacat cgtcatcttg caaaacaatt
      181 tagacaaatg ctcgggcaag actacagagg agtgcaaaat gataaatcag ctcgcgggca
      241 agtttaacga atttaacgga gccgccaaaa agttctcatc agaaatagat acggccagaa
      301 atttctgcag taacccgagc gaggcaagct ttgagaaaat agcggaatct ttacaaggaa
      361 tcaaagaaga tggagaagac ctccgaatat taggaaaaga attacaagcc gaccaagctg
      421 aaaaagcgag cgagaaggct ctttgcaggg cgatcgttcc cctctttgcg acaggcaaga
      481 gcgaattggc cgcagggcta gcaaaggtca aggcgaccca agacagctgt tccggtagca
      541 aggacgagaa gtgcgcatct ataagagccc ttgcgccaaa gttctctgaa gtgtccacaa
      601 aaggaaacga attgctcaca ggaatgacga gcgtcgagac cacctgcaaa acaccgagcg
      661 acaaggcgcc atcggccgac ttcataaaga aaatagaagg cataagaaat agcaaagacg
      721 ctgtcttaaa actcgttgaa gccctaaggg ccgagcagga caaggcggct actagcggca
      781 agggaataag agttgaagcg gaagaagaag tctccacaaa cttactgcca aagacagaaa
      841 gctggcactc aagaaaagaa ttaaacccaa gctggcgacc accattcttc ggcgcgggtg
      901 tttggtattt gtctagagga ggagaatcgc tgtcatacaa cgtaaatgtt ccgaccgcag
      961 gagattacag cttgtgggtt agagattatg tggacaattt ccagccgaaa ggggtaagaa
     1021 gagtcacata tacgataaac ggcaaatcac tggggacata tcctgaagta gcgctggccg
     1081 gaagcaaaat agatgcgggg aagggcgcat tcggatggca caaggtattt gtcacaaaat
     1141 taaatgccgg accgaatatg atgaaagtta caaaagaatc aacgacgtca ggtgcggcga
     1201 tacttgatgc gttttacttc acaaccggca gtgacgttcc tccggaaaag taaaataaga
     1261 attatgaatc aagaattaag aataatgaat aaaaaaatga cactaaataa aaaaaataat
     1321 caaggaatcg cgccgctcat aataattctc atcgtcgccc ttcttgcagg tggaggcgga
     1381 tatacctatc taaaatcaaa aaatatcagc ctgccctctc tcggactcgg cagtagcaca
     1441 gcaaagaact tggatgagat gacgatagac tccggcaata tggactttag cctatcaact
     1501 atcggcgcgc tcgacgtggc tcccctttct ctagaaatac caaacttgag cgctcccgct
     1561 atcgagatac caaagacaag cgtggacatc gggacattca acacaagcaa cataaacatc
     1621 ggcatgccgg atctttcgag cgtaatgccg gccgtttcga tcccctccgg tattcctgcc
     1681 aatattccca taccgtcagc accgacaagc attccaacaa gtattccgac agatatccca
     1741 ccatcagcac cgacgggcgc aagtggagcc cctgcgggca tgcctacggg agccggagcg
     1801 cctagcgtag actgctctgc attcgcttct gtcccaagct gttcaataat aacaggcccg
     1861 ggaggggaaa tgtgcaaaca atgcttcccg aataaataat ccctcgacac caaaccgtgt
     1921 cattcccgcc cccgatcgcg gtcgagggta aactccggcg ggaatccaga aaattgtgga
     1981 ttaccacggc tcggctgatg ggcctcgcaa tgacaatact gaacgaacaa agaaaaaccg
     2041 tcccgataaa atcgaggacg gtttgatatt gtccgcaaac tactattttt cgcttttgcc
     2101 gataaggaaa ttgatccctt cggccagact ttcgtcatta acgatgtcct ggaaggaaac
     2161 gcccttttcg actgaaagtc gatggatgat ctcggcacgc ttccagagat aattttgaac
     2221 gtttttacct gcgaagaaga tgtgcatggt aacttccaaa aggacacaaa gatcttcagc
     2281 gcttccacta aatttaaaat cttcatgctt ccgaacaaca cgattaagga tcctcttggc
     2341 gtccaattcg ctaaagatgc gggcatagga atctccgcgc ctcaggtagc gaacaaaaca
     2401 ccaacaaact tcttctttta actctttata ttcatcactg ggaagaagct tattcatcat
     2461 cgccagaagg tcctggtccg agaccttggc cttatcgaat tcgctcatca tgcctcccta
     2521 ttcttcggca tttaagaaat ttagaagttt ttcaaatggc cggagacagc gttcaacgaa
     2581 cgaaatatca attctcagcc tttcaaagac cgccaatgcc ctagtgcctg cccaaatgac
     2641 caggaggatg gcgcccgtta aaagagtaca atagccgcta atgaggatgc cggctagcac
     2701 accattcaac aggccaaagg cgattccctt gataacactc aaaagtaggc cgaacaggat
     2761 aaaacccgct atcaaactaa gggcaaatga aaataccccc gacttgagaa tatcactgaa
     2821 acaaagattg acaatggggc gattatattt atcaccttcc gtttccgtcc actcatttat
     2881 cggcagaaac acgagcaaga agaaggcgcc gaagatcggc catgcgagag ggtcaaacca
     2941 cctcgtgaac tcgaagagcg gaacggcatc cttgccgtcc atagggaaag acacgagatc
     3001 tttcgggacg ttcataccgt agaaataaat gagcagggca aagtgaacga gcgcaaagag
     3061 caccgaccag cgaaatactg cctttttgat cttttctttc ggcatcataa cccctcctct
     3121 ttcttttcct tattctttct tcttcgcatc ttcattttct aatgacgaca aattcatcca
     3181 atcagagaat aaaataaaca tgctgaaatt ggccaggacc ctcagaaaaa ttccgaaatg
     3241 ataaaaaggg tcaccccaaa atccaaatgc aaaaaagtgg agtaatattt caactagtgt
     3301 caaaaaaagg ctattttaac aatcaccggg aaaaaattcc caagtcgttt tgtcgtcata
     3361 aagaccccct tcttttgagc cgcaaagcgg ccaattcaca ttaattttca acgaccaagc
     3421 cctacacaaa actaccacat aaatagagtt gagtaaatac acccagatta tcgtgcccca
     3481 gctaaaagcc gacggcccgc tgataattaa aaggccttat ttttagccat gatttgacct
     3541 atttttatgc tcaaaaccat tgacatttaa gacgtaatat gctatactta aaaaataaga
     3601 tgattcgcac gaaagtacga atttttcttt aaatatatga taaacacaat aattacaaat
     3661 acttttgtcg gagttctgct ctttgcctct tctacctctt taaccccgac agatctatca
     3721 aaaatgatag aatcatcctc tgctaaacag gaggttgaga taaagtgcga tacaaaagaa
     3781 aaggaatgta taatcgctct tatcgagaaa tattcaaaag aatttgatta ttctccgaaa
     3841 atggcaaaag ctatcgccta ctgcgagtct gaatataaag agactgcaaa aggtgacaaa
     3901 ggaaaggctt acggaatata tcaattccac aaaccgacct ttgaaatgtt tgcaagacag
     3961 aaaggtgaaa agttagatta ttatgatacc gaagacaata tcaaactagc catctgggca
     4021 ctcgccaacg acaaagaaca tcactggtcc tgctacaaga aagtggctac aagataaccc
     4081 cggtagtaga gcaaagctca ctacagggca ggtctaaaaa aatcctcgcg caatgcgggg
     4141 attttttgtt aattaaacgc aagacagtaa aaactggatt cccgtcttca cgggaatgac
     4201 aaaggcgagt agttgttgtc atctcgagct tgtcgagaga tcgcttatag tccaaatacc
     4261 tagaggcgaa gcgattcctc gacgggctcg gaatgacaaa agtggagggg accaaatata
     4321 gaagccttag agtaaatttt ttagagacaa aacatg
//
LOCUS       DAOTEX010000061         4334 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3118
            NODE_14877_length_4334_cov_4.385137, whole genome shotgun sequence.
ACCESSION   DAOTEX010000061 DAOTEX010000000
VERSION     DAOTEX010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868142
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4334)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4334)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 883
            CDSs (total)                      :: 833
            Genes (coding)                    :: 829
            CDSs (with protein)               :: 829
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4334
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14877_length_4334_cov_4.385137"
                     /isolate="AMR_MDS_3118"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..806
                     /locus_tag="P5056_03750"
     CDS             <1..806
                     /locus_tag="P5056_03750"
                     /inference="COORDINATES: protein motif:HMM:NF024108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TraM recognition domain-containing protein"
                     /protein_id="HRY62855.1"
                     /translation="DEFRERKLARIKNPSVIDFWEKEAAKTTGDWSLANMVGYIASKF
                     DNFISNHYMRPIIGQTESAFNFREVMDEGKILLVNLSKGKIGDINSSLLGMLITGKIL
                     QAALSRQELIEKGQKDFKDFYLYIDEFQNYTTDSIATILSEARKYKLNLILAHQFIQQ
                     LDEKIRDSVFGNVGSMLAFRVGAPDTEMLLKQFSPEFNEKDLISIDNLNCFGKILIGG
                     QPSRPFNMKISLPETGNSVVREKLKELSRLTYGKDATEVENEIVARLRG"
     gene            complement(875..2014)
                     /locus_tag="P5056_03755"
     CDS             complement(875..2014)
                     /locus_tag="P5056_03755"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRY62856.1"
                     /translation="MFLKEVVIKYLKIDKNKMFNPNQIPSPTIRNPEPISLKEYADQS
                     SKDLRSKNLENINIDVFETWGFDTNTLWPEKMPPEIKPTETLENGKNPGLGIRQLHKE
                     GITGKGVNVAIIDQILKIRHKEYSDSLVHYEEVDDLSEEPQTMHGPGVSSVFVGKECG
                     VAPEAKLHYFAKLSGEDYADKKYYTKAIKKVLEYNANLPEQEKIRVLSISKGYMNKSP
                     ELKTDEDIEWETILKTAERSGIMVIWVSMNEYGDNFPSSGTGSNIFDKDDPDGYDEWL
                     SIKHRGLKKSDQAKENEILIPCDHRTYASPIGEGEYAHSGEGGASWKVPYLAGIFALA
                     LQIDPELKRAEILEKIKSTATKNKRGLKIINPRGLIDLIKKEKTS"
     gene            2263..3318
                     /locus_tag="P5056_03760"
     CDS             2263..3318
                     /locus_tag="P5056_03760"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HRY62857.1"
                     /translation="MAEKPLFYKLLSVKPISLFLALSFFLGSAAPAHAGVLSDILGLF
                     GSASAEVKETISDNNSQTASLLKPMTAVLVSAEGETPIVEDSALMSDSGPMGTIADVE
                     YLTPADNKIEVYVVKKGDSLSSIAKMFGVSSKTIMWANDIANEKSLKLGQTLVILPIS
                     GVKHTIKKGDTVQSIAKTYGGDVEEILKYNYIDAKDKLVVGDTVIIPDGDIKTVVSKS
                     SSGNSSRVAKNLPSYAGYYIKPAVNVRRTQGLHGNNGIDFAPYSKTSGTEPIYAAASG
                     NVIIARTGGWNGGYGNMIVISHSNGTQTLYGHCYNIFVNQGEYVEQGQIIGFIGNTGK
                     STGPHLHFEVRGARNPF"
     gene            3510..>4334
                     /locus_tag="P5056_03765"
     CDS             3510..>4334
                     /locus_tag="P5056_03765"
                     /inference="COORDINATES: protein motif:HMM:NF021831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ser-Thr-rich GPI-anchored membrane family
                     protein"
                     /protein_id="HRY62858.1"
                     /translation="MKKIFLVIVAFFLMFAFSPIARAQGNAEDLSLLQRILEQAKVLQ
                     QKLIEREAALSIYQMKVISPNGGETIRAGQYTDIKWDATNAGVSKIDIYLTTSADEGN
                     RKDPGFTIAKDVPLETPFYHWLVPNNLAGQGYKIFIRNNGKSYRLGDYSDAVFNISAP
                     EAGVSSVKLNSPKGGEMYIAGRTYSIHWNMPKGSDRYVSIKLIKGDQDSSDINYVSAG
                     LEEYIPASQGSTEWLVPDFVTSGDYKMKIIGLSNLSCNVTESSNIYTSGVFKIVKDT"
BASE COUNT         1178 a          906 c          938 g         1312 t
ORIGIN      
        1 atgacgaatt tagagagaga aaactagctc gaattaaaaa tccttcagtt atagatttct
       61 gggagaaaga agccgcgaaa acgaccggag actggtcact cgcaaatatg gtcgggtata
      121 tcgcctcaaa gttcgacaac tttatttcca atcactacat gaggccgata atcggtcaga
      181 ccgagtcggc gtttaacttc agagaagtga tggacgaggg aaagatcctt ctcgtaaatc
      241 tttcaaaagg aaagattggc gacataaact caagtctcct cggtatgctt ataacaggaa
      301 agatccttca ggccgcgcta tccaggcagg aactcatcga gaaaggccaa aaagacttca
      361 aagattttta tctatatata gatgagttcc aaaattacac gactgactct atcgcgacga
      421 tactttcgga ggcaaggaag tataagctga acctcattct cgcccaccag ttcatccaac
      481 agctcgacga gaagataagg gattctgtat ttggaaacgt cggctccatg ctcgccttcc
      541 gagtgggtgc acctgacacc gagatgcttc tgaagcagtt ctccccggag tttaacgaaa
      601 aagacctcat aagcatcgac aacctaaatt gcttcggcaa gatccttatc ggcggccagc
      661 cttcaaggcc gttcaatatg aagatatctc tgccggaaac gggcaacagc gtcgttagag
      721 agaagctaaa ggagctttct cgcctcactt acggcaaaga tgccacggaa gttgaaaacg
      781 agatcgtggc acgactacga gggtagaaag tggagagtag aaggtggtca aataaaatta
      841 gaaaaggccc cctaagaggg cctttttagt tggtttagga tgttttttct tttttaatca
      901 gatctatcag cccgcgagga tttattattt taagccctct tttgtttttt gtggcggttg
      961 atttaatttt ttccaaaatc tcagctctct ttaattcagg atcaatttgt aatgctaacg
     1021 caaagatgcc ggcgagatat ggaactttcc aacttgcccc gccttcccca ctgtgagcat
     1081 attccccctc tcctattgga gatgcgtatg ttctatggtc gcacgggatt agtatctcat
     1141 tttcttttgc ctggtcggat tttttaagac ctcgatgttt gatgctaagc cattcgtcat
     1201 atccgtcagg gtcatcctta tcgaatatat tactgccggt gccagagcta ggaaagttgt
     1261 ctccatattc attcatactc acccatataa ccattattcc cgaacgttcc gctgttttta
     1321 atatcgtttc ccactcaatg tcctcatcgg ttttaagttc aggcgattta ttcatatatc
     1381 ctttcgagat agagagaacc ctaatctttt cttgttcggg gagattggcg ttgtattcta
     1441 atactttttt tatagcctta gtatagtatt ttttatctgc ataatcttcg cctgataatt
     1501 tggcaaaata atgcagcttg gcttccgggg ctacgccgca ttccttgccg acaaacacac
     1561 tcgatactcc aggaccgtgc attgtttgtg gttcttcaga taggtcgtct acttcctcat
     1621 agtggacaag actgtcggaa tattctttgt gccggatttt tagtatctga tcgataatgg
     1681 cgacatttac ccccttacca gttatcccct ctttgtgtaa ttgcctaatg cctagtcccg
     1741 gatttttccc attttccaga gtttctgttg gttttatttc cggcggcatc ttttccggcc
     1801 aaagcgtatt tgtgtcaaat ccccatgttt caaaaacatc aatgttaatg ttttctagat
     1861 ttttagaacg taaatctttg gagctctggt cggcatattc cttaagagat atcggctccg
     1921 gatttctaat ggttggtgag ggtatttggt ttggattaaa catcttgttt ttgtctattt
     1981 taagatattt tatcacgact tcttttaaaa acatattttc tctcgttttt gtagtaaacc
     2041 aatatttttt ccacttaaaa tatccttatt tctagccata taatcataat tttcgttgac
     2101 tttgcttgct aattttgcta ggattaaaga tacgagtgca aggttgcatg tttggttgag
     2161 tggctgtgac aagggccaat tcactgaaac ttctagaaaa tccttaattt taggcttatt
     2221 atagcaaaaa ctaataaagc tagccgacac cagcgtgtcc gcatggctga aaaaccgtta
     2281 ttttacaagc ttctttctgt gaagcctatt tctctatttt tggcccttag tttcttccta
     2341 ggttctgcgg cacctgctca tgcgggtgtt ttaagtgata tcttggggtt gtttggtagc
     2401 gcaagcgccg aggtcaagga aaccatctcc gacaacaatt cccagaccgc ttccctcttg
     2461 aagcccatga ccgccgtgct cgtttcggcc gaaggagaga ctcctatagt agaggattcc
     2521 gctcttatgt ctgattccgg cccgatggga actatcgccg acgtggaata ccttactccc
     2581 gccgataata agattgaagt atacgttgta aagaagggcg attccctcag ctccattgcc
     2641 aagatgtttg gcgtttcttc taagaccatc atgtgggcca acgatattgc caacgaaaaa
     2701 agcttgaaat tgggccagac gctcgttatt ctccctattt ccggcgttaa acacactatt
     2761 aagaaaggag atactgttca gagtattgcc aagacctatg gcggagatgt agaagaaatc
     2821 ttgaaatata attatattga cgctaaggat aaattggttg ttggagatac ggtcatcatt
     2881 ccggacggag atattaagac cgttgtttct aagtcctctt caggaaattc ttcaagggtg
     2941 gccaagaacc taccttctta tgccggttat tatataaaac ctgcggttaa cgtcagaaga
     3001 actcagggtc ttcacggaaa caacggtatt gactttgccc catattcaaa gacttcagga
     3061 acagagccta tttacgccgc cgcttcgggt aatgtgatta tcgccagaac gggcggatgg
     3121 aacggtggat atggaaatat gatagttatt tctcactcaa acggcacgca gacactttac
     3181 ggccattgtt acaatatttt tgtaaatcaa ggcgagtatg ttgaacaggg ccagataatc
     3241 ggatttatcg gaaacacagg taaatctaca gggccgcatc tacattttga agtacgaggc
     3301 gcccgcaatc ccttctagat aaaactttcg cgtaaatcca ctcaatattg tcattcccgc
     3361 cccccaatcg cggtcgaggg tagattccga cgggaatcca gtttttatga aatatcaaca
     3421 ttattctcgc ccttatgcac agggcgtttt ttgtttttgt ggaagcggat gttataatta
     3481 tattgaataa ttgcgttaat tttttttcta tgaaaaaaat atttttagtt attgtggcat
     3541 tctttttgat gtttgccttt tcccctatcg cgagggcgca gggcaatgcg gaagacctct
     3601 cgttactcca gaggatatta gagcaggcca aggttcttca acaaaaactt atcgagagag
     3661 aggcggcttt gtctatttat cagatgaagg ttatttctcc gaacggtgga gagaccataa
     3721 gggccggcca atatacggat attaaatggg atgccacgaa cgccggtgtt tcaaaaatag
     3781 atatttatct tacgacttcc gctgatgaag gaaacaggaa agatccgggg tttactatcg
     3841 ccaaggatgt tcctttggaa actccgtttt atcactggct agttcctaat aatctggccg
     3901 gccagggtta taaaattttc ataaggaata acggcaaaag ttatagactt ggtgattaca
     3961 gcgatgccgt ttttaacatt tcagctcctg aggctggtgt gtcgtcggta aaattgaatt
     4021 ctccaaaagg gggagaaatg tatatcgccg gaaggacata cagcattcac tggaatatgc
     4081 cgaagggaag cgatagatat gtttccatta aacttataaa aggagaccaa gattcatctg
     4141 atataaatta tgtatccgca gggttggagg agtatattcc cgcctcgcag ggatctacag
     4201 agtggctggt gcctgatttt gtcacatcgg gagactacaa gatgaagatt atcggattaa
     4261 gcaatttgtc ttgtaatgta accgaatctt caaatatata tacatcaggc gttttcaaga
     4321 tagttaaaga tacg
//
LOCUS       DAOTEX010000062         4143 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3118
            NODE_15838_length_4143_cov_5.112524, whole genome shotgun sequence.
ACCESSION   DAOTEX010000062 DAOTEX010000000
VERSION     DAOTEX010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868142
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4143)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4143)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 883
            CDSs (total)                      :: 833
            Genes (coding)                    :: 829
            CDSs (with protein)               :: 829
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4143
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15838_length_4143_cov_5.112524"
                     /isolate="AMR_MDS_3118"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..557)
                     /locus_tag="P5056_03770"
     CDS             complement(<1..557)
                     /locus_tag="P5056_03770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62859.1"
                     /translation="MKNLKYIVALVALVAIPALSFAAWSAPTAAPTSDNTQPPLNIST
                     ADQEKLGTITAKGLCVRLSATTIRCLGETPGNTPSVFDFPWIQQGNNIVNTNTGNVLV
                     NPLRINNLWGTAPTERLTVGGKVKAEGLCLGLSCIDINTGGTGDTVNNWKEAITSLIG
                     GTGAGAGTVGTLAKFTGVTTLGDSS"
     gene            complement(652..912)
                     /locus_tag="P5056_03775"
     CDS             complement(652..912)
                     /locus_tag="P5056_03775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62860.1"
                     /translation="MKAIKIIVTIVLLFAAVYASTLVYKYFSIKTQEEIERDAILGAL
                     SSPVVPINEAETRAVLEAMKQTAPTEITKQEAQNLINVLKQY"
     gene            1000..1683
                     /locus_tag="P5056_03780"
     CDS             1000..1683
                     /locus_tag="P5056_03780"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRY62861.1"
                     /translation="MLVSITMSTPIREFANEEYYHVYNRGADKRDIFLDEYDLKRFIQ
                     AMQEFNTTEPTGGIYLNSFPKNNLQLRPLGSKLVEIIAYCLNPNHFHILLKQSSDNGI
                     SLFMSKLGGYSKYFNLKNKRTGTLFQGTFKAKHISDNEYLLNLSAYINLNDTIHQLGP
                     LGSKLVERSSWAEYINPGKKELCTKDIILKQFQNPNDYRKFAESVASEFIQHKKDIKD
                     ADNLTIEVI"
     gene            complement(1712..3424)
                     /gene="recJ"
                     /locus_tag="P5056_03785"
     CDS             complement(1712..3424)
                     /gene="recJ"
                     /locus_tag="P5056_03785"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HRY62862.1"
                     /translation="MSKWRLREKINEKDTELFKYYPEIIACALFHRGIRTAEEARKYF
                     DPSYDDLPDPFLMTDMMKAAVRIVAAIKNKERIVIYADYDCDGIPAATIIHDFFKKIE
                     FNNFEVYIPHRHNEGYGMNLESVRELCARGADLFITVDCGITDFEATDEANMLGADVI
                     ITDHHEPKDILPNAFAVVDPKREGDKYLCKTLSGSGVAFKLIQAIIILDRFGISEGWE
                     KWLLDMAGLGTIADMVPLQGENRVIAQFGLSVLRKTKRPGLLNMYRKSRLEPRNITEE
                     DIGFSIAPRINAAGRMGVPSVAFTFVSSVDHEEASGAFDYLELKNNERKSLVTDVMKE
                     AAEIVKNLQHKNILFVGKEGWNPGILGLVAGRLSDNLGKTVFVWGGEGECFKGSCRSD
                     GSVSLVRLMGELSSEIFADFGGHHLAGGFSLNKNFINTASEMLEEAYSKVADEKKEDE
                     IMIDEILRPDDVTWKLYDSLEKMAPFGIGNPRPQFLFENVRICGTKKFGKKNDHFQIE
                     ILTDNGGRIVAYDFFIDENSFSDVVLRPGALVSLVATIEKNTFGREPTLRLKIIDIKP
                     AGHN"
     gene            3656..4081
                     /locus_tag="P5056_03790"
     CDS             3656..4081
                     /locus_tag="P5056_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013451074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HRY62863.1"
                     /translation="MDKFKNKDCIFCKIVAGEIPAEKIYEDDDLFAFLDITPINAGHT
                     LLIPKKHYENIFEMDDEILKKISIKLRDLGTSVKGAMKAGGLNIGMNNSPVAGQVVPH
                     AHFHLIPRYLKDGHKHWEGRKYKEGEITKVAGEIRGKVR"
BASE COUNT         1230 a          955 c          772 g         1186 t
ORIGIN      
        1 atagatgaat cccccaacgt cgtcactcca gtaaacttcg caagtgtgcc gacagttccg
       61 gcacctgcac ctgtgccacc gatcagactt gttatcgcct ctttccagtt gttcacagta
      121 tctccggttc ctcctgtatt tatatcaata cagctcagac caagacagag tccttcagct
      181 tttaccttcc cgccgacagt gagcctctct gtcggagcgg tgccccagag attatttatt
      241 cttaaaggat taacaagaac attccctgtg ttcgtattta ctatattgtt tccttgctga
      301 atccaaggga aatcaaatac ggacggcgtg tttcccgggg tttctccgag acatcttatc
      361 gttgtggcgc tcaatctgac acaaagtccc tttgcggtaa ttgtgccgag cttctcctga
      421 tctgcagtac taatgtttag cggtggctgt gtattgtccg aggtaggagc tgctgtaggc
      481 gcgctccatg cggcgaaaga aagtgccggg atggcgacaa gagcgactaa ggcgacaatg
      541 tattttagat tcttcataaa ttatttttta tttaccctat ttagtaaaaa attgattgtg
      601 attaaatttt taatttttta aaaaaatttt atttaacagg gttaattatt gttaatattg
      661 ttttaaaaca tttattaaat tttgagcctc ttgcttcgta atttctgtcg gtgcggtctg
      721 cttcatcgcc tcaagcacgg cacgtgtttc cgcttcgttt atcgggacaa ccggggaaga
      781 caaagctcct aaaatagcgt ctctttctat ttcttcttga gtcttgatgg aaaaatattt
      841 ataaaccaaa gtgcttgcat aaacagccgc aaaaagtaga acgatagtta ctattatttt
      901 tatagctttc atatataaga ttatatcacg acaagaaatc agataacacc cccctatatc
      961 cccacacctc ccccctactt agacccgagg ggtctaagta tgttagtatc aataactatg
     1021 tctacaccaa tcagagagtt tgcgaacgaa gaatattatc atgtgtataa caggggcgcg
     1081 gataaaagag atatttttct tgatgaatat gatctcaaaa gatttattca ggccatgcaa
     1141 gaatttaaca caaccgagcc taccggcgga atatatctca actcatttcc caagaacaat
     1201 cttcaactga gacccctcgg gtctaagttg gtagaaatta ttgcatactg ccttaatccc
     1261 aatcattttc atattctcct aaaacagtca tccgacaatg gcatcagcct ttttatgagt
     1321 aagctaggcg ggtattctaa atatttcaat ttaaaaaata aaagaacggg aacccttttt
     1381 cagggaacat tcaaggctaa gcatatctcc gacaatgagt atcttttaaa tctcagtgca
     1441 tatataaatt tgaatgacac aatacaccaa cttggacccc tcgggtctaa gttggtagaa
     1501 cgttcaagct gggcagagta tataaaccca ggcaaaaaag aattatgcac aaaagatata
     1561 attctaaaac aattccaaaa ccctaatgat tatcgaaaat ttgctgaaag tgtcgcgtca
     1621 gaatttatac agcacaagaa agatataaaa gatgcagaca acttaacaat tgaagtaata
     1681 taaaacttag acccctcggg tctaagtttt actaattgtg gccagcaggc ttaatatcga
     1741 taattttaag gcggagcgtc ggttctcggc caaaagtgtt cttttctatg gtcgctacaa
     1801 gactcacaag agcgcccggc cttagcacaa catccgaaaa gctgttttcg tctatgaaaa
     1861 aatcatatgc gacaatcctc cctccgttat cggtcaatat ttcaatttga aaatggtcgt
     1921 tcttcttgcc gaacttcttc gtaccgcata ttcgcacatt ttcgaaaaga aactgcggac
     1981 gaggattacc tatcccaaac ggagccattt tttccaacga atcatataat ttccatgtca
     2041 cgtcgtccgg tctcaatatc tcgtcaatca ttatctcatc ttccttcttt tcgtctgcca
     2101 ccttcgaata agcctcttcg agcatctcgc tcgcggtatt tataaaattt ttattcaaag
     2161 agaaacctcc ggcgagatga tgcccgccaa agtcggcaaa aatttccgag gacagctccc
     2221 ccatgaggcg aacgaggctt accgagccgt cagatctaca agagccctta aagcattccc
     2281 cctctcctcc ccacacaaaa accgtttttc ccaaattgtc ggacaatcgc cccgccacaa
     2341 gccccaatat gccggggttc cagccctctt ttccgacaaa tagtatattt ttgtgctgta
     2401 aatttttaac aatttccgcc gcttccttca taacgtcggt gacaagcgac tttctttcgt
     2461 tgttctttaa ttcaagataa tcaaaagccc cgctagcctc ctcgtggtcg acgctggaca
     2521 caaaagtaaa agcaactgac ggcacaccca ttctgccggc ggcattgatg cgaggcgcta
     2581 tagaaaatcc gatatcttct tccgtgatat ttcttggctc aagcctactt tttcgataca
     2641 tattaagaag acccggcctt ttggtcttcc ttaaaacaga gagaccaaac tgcgcaatta
     2701 cccgattttc cccttgtaac ggcaccatgt cggcaattgt ccccagtccc gccatatcta
     2761 aaagccactt ttcccacccc tcgcttattc caaacctatc caaaataata atagcctgga
     2821 tgagcttaaa tgcgacaccg gaaccggaca acgttttgca aagatatttg tccccttccc
     2881 tctttggatc aacaacggca aacgcattcg gcaaaatatc tttcggttca tggtgatcgg
     2941 tgataatcac atctgcgccg agcatattgg cttcatccgt ggcctcaaaa tcggtaattc
     3001 cacaatcaac ggttataaaa agatctgccc cgcgagcgca aagttcgcgt acagattcga
     3061 ggttcatacc gtatccttca ttgtgtctgt gcggtatata tacttcaaaa ttattaaatt
     3121 ctattttttt gaaaaaatca tggatgatcg tagccgccgg tataccgtcg caatcataat
     3181 ccgcataaat aacaattcgt tctttatttt ttatcgcggc cacgatacgc accgccgcct
     3241 tcatcatatc ggtcattaaa aacgggtcgg gcaggtcgtc gtagcttgga tcaaaatatt
     3301 tccttgcttc ttccgcggtc ctaatcccgc ggtgaaatag ggcgcacgca attatctccg
     3361 gataatattt aaaaagctcg gtgtcttttt cgtttatttt ttctcttaat ctccacttcg
     3421 acataggcgg attatagcac tcatgattat ctacaaaaac ttgttacttt tatcaataaa
     3481 tagcatcgtg tatttaacga acctgccccg taggaagctg caaaaataac aaaaataacc
     3541 tccaataaaa gccgctaggc ggattccgag aaataaaatt aataataaaa aaataaccta
     3601 ttcgagcttt ccttcggggt ttacccgaac acttgtttat tatatattta atgtcatgga
     3661 taaattcaaa aacaaagact gcattttttg taaaatagtg gcaggcgaaa ttcccgccga
     3721 aaaaatatat gaagacgatg atttgttcgc ctttttggat atcactccta ttaatgccgg
     3781 ccacactctg ctcattccca aaaagcatta tgaaaatatt tttgagatgg atgatgagat
     3841 attaaaaaaa atatctatca aactacgaga cttaggcaca tcggtaaaag gagcaatgaa
     3901 ggccgggggg ctcaatatag gtatgaacaa cagcccggtg gcgggccaag tagtccccca
     3961 cgcccatttt caccttatac caagatacct aaaggacggc cacaaacact gggaaggaag
     4021 aaaatacaaa gaaggagaga ttacaaaagt ggcgggagag ataaggggga aggttagata
     4081 gtctagactt tttaataaga atattgtaga ttcttataac gagttgcgcg gttagctcag
     4141 tgg
//
LOCUS       DAOTEX010000063         4012 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3118
            NODE_16561_length_4012_cov_3.784938, whole genome shotgun sequence.
ACCESSION   DAOTEX010000063 DAOTEX010000000
VERSION     DAOTEX010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868142
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 4012)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4012)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 883
            CDSs (total)                      :: 833
            Genes (coding)                    :: 829
            CDSs (with protein)               :: 829
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4012
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16561_length_4012_cov_3.784938"
                     /isolate="AMR_MDS_3118"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(191..535)
                     /locus_tag="P5056_03795"
     CDS             complement(191..535)
                     /locus_tag="P5056_03795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62864.1"
                     /translation="MTKLEIKLKNKIMRRVYAVWLLKKIISLAFLRFIVLCGFAFEFA
                     REVSVISVFGNLPAVTDFGANFNYFAFAFSHTELSVQIYLVGIITLVFWYALHETAKI
                     ATQYNLILSNRN"
     gene            complement(537..1127)
                     /locus_tag="P5056_03800"
     CDS             complement(537..1127)
                     /locus_tag="P5056_03800"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HRY62865.1"
                     /translation="MLLLEDRDLNIDYKDHKDEEVLALSIANPNIFGVIVERYEEAFL
                     RKAKSIIGYDIDEVNDIVQETFTKIYLNAHKFRPVPGASFKSWAYRILLNVTFTWYGK
                     LKKGREAVMRLDPELDEVIPDKKASSEFERETTKDYVISVLVRMPDSFAKVLRSYFIE
                     GKSHKEIAKEEGVSVEAIKTRMCRAKKEFKKIECNI"
     gene            1632..2195
                     /locus_tag="P5056_03805"
     CDS             1632..2195
                     /locus_tag="P5056_03805"
                     /inference="COORDINATES: protein motif:HMM:NF001810.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HRY62866.1"
                     /translation="MLEYNEITLRKFIIFENEPFEVLDSHVFRKQQRKPVNATKLKSL
                     LSGRIVEHSFHASDKVEEAEIDTKLAKFLYANRGEYWFCDPKDPSKRYTLPESLLGDA
                     AKFMKQNSEIETKLFDEKVIGVKLPIKVELKVKESAPGVKGDSAKGGMKQATLETGAV
                     INVPLFVNEGDILRINTETGEYVERVN"
     gene            complement(2305..2505)
                     /gene="rpsR"
                     /locus_tag="P5056_03810"
     CDS             complement(2305..2505)
                     /gene="rpsR"
                     /locus_tag="P5056_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HRY62867.1"
                     /translation="MKQCHFCVNNVKSIDYKDTESLGKFLNPHSSILRSWRTNTCSRH
                     QRKTATAVKRARFLGLLPYVSR"
     gene            complement(2595..3044)
                     /locus_tag="P5056_03815"
     CDS             complement(2595..3044)
                     /locus_tag="P5056_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HRY62868.1"
                     /translation="MYLNKAIIIGNMTKDPEMRALPSGQKVTSFSVATNRVWKDKDGK
                     KQEGTEFHNISVFGRQAETCAQYLKKGQAVLVEGRIQTRNWDDKDGVKKNRTEIIAEK
                     VQFGPKAGGSVGGSTYPSKNSAPAESNSVGQQIDYPEEEINPDDIPF"
     gene            complement(3065..3520)
                     /locus_tag="P5056_03820"
     CDS             complement(3065..3520)
                     /locus_tag="P5056_03820"
                     /inference="COORDINATES: protein motif:HMM:NF013419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HRY62869.1"
                     /translation="MDSSDKNVYELGYLLIPIIAPEKLADEVNAIRALVESNGGLVLF
                     EEAPKERKLAYEMSHDITGGRRAKYDDAFFGWMKFNLSADAVSKLDAGLKNNQSLIRY
                     LIINLTKEGGDSNGAAEAKKPLGVGKKPKKTVSEKELDKEIDNLLDKTA"
     gene            complement(3811..4002)
                     /locus_tag="P5056_03825"
     CDS             complement(3811..4002)
                     /locus_tag="P5056_03825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020255020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HRY62870.1"
                     /translation="MRGTIKLLKNGFGFIGIEGAKDVFFHAKDLNGVAFDSLKVGQEV
                     TFEMGESPKGPKAEMVTLA"
BASE COUNT         1173 a          880 c          715 g         1244 t
ORIGIN      
        1 gccactctct actgtctaca atctaccctt caaacgaacc ccagtgaaat aagcgccaat
       61 tctctacatt tttgcaaaag tgaaaatata cccaattgaa caaggcatcg gtggtgacat
      121 taaatcgatt tcacggggtg aacatctaaa ggcccctgtt tatttattcg caggggctta
      181 tagttaacta ttaatttctg ttgctcaaaa ttaagttata ctgagtggct atcttggcgg
      241 tttcatgcaa ggcataccag aataccaaag taatgatccc cacgagatat atttggacag
      301 acaattcagt gtgagagaat gcaaatgcaa aataattgaa attcgcgcca aaatccgtta
      361 ccgcgggcaa gttgccgaag acgctgatca cggaaacctc tctggcaaac tcaaaagcaa
      421 aaccgcaaag cactataaat cttaaaaagg cgaggctgat tatctttttg agtagccata
      481 cggcatacac ccgcctcatt attttatttt taagttttat ttctagcttt gtcatattat
      541 atattgcact ctatcttctt aaactccttc ttcgccctac acattcttgt ttttatagct
      601 tccacagaca cgccctcttc tttagctatt tctttatgcg attttccttc tataaagtaa
      661 gagcgaagaa ctttggcgaa cgaatccggc attctgacca aaactgaaat aacataatct
      721 ttagttgtct ccctctcaaa ctcactgctc gccttcttgt ccgggataac ttcgtctaat
      781 tccggatcta atctcattac cgcctctcta ccctttttta actttccata ccaagtaaac
      841 gtaacattca aaagaattct gtatgcccaa gatttaaaag atgcgcccgg cacaggccta
      901 aatttgtggg cgttaagata tatctttgta aaagtctcct gaacaatgtc atttacttca
      961 tctatatcgt atccgattat actcttcgcc tttcttaaaa acgcctcttc atacctctca
     1021 acaataaccc caaagatatt tggattggca atagaaagag ccaggacctc ttcatccttg
     1081 tggtccttat aatcaatatt taagtctcta tcttccaaaa gcagcattgt tgtttattta
     1141 ctattgtttc ttacacttat tgtaacaaat tcgaccggga aaaagtgtcc cgtaccccct
     1201 aagataacca cctacaaaac aatatgtcaa atgcagaaaa acgaccttcc cataaggggg
     1261 gttgtaaaat aagcatattt cggatatgat gaaccaactg agtgattcac cctgttaagt
     1321 agtccaattg agtaaaattt tattttaaaa taaaatttta ctcaattgga taaaatttgt
     1381 ttatagcggg gtaaataacc aaaaggaagc ccgtcgaaat attagaaaaa taagattata
     1441 agtaaaaaca attaaacaaa tttttactta acggcttaga gttcaccctg ttaagtaaaa
     1501 tctttttaaa aagatttaaa atttgtttag taatgtgaga tttatcgggg taatatgata
     1561 gtcgaaaata ttaagaattt taaataatat taggtaaaaa caattaaaca aatttttact
     1621 taacagggta aatgttagaa tataacgaaa ttacattaag gaagttcata atcttcgaaa
     1681 acgagccatt cgaagtatta gattctcatg tttttagaaa acagcagaga aagcctgtta
     1741 acgccacgaa gctgaagagt cttttgagcg gtagaatagt tgaacactca ttccacgcca
     1801 gcgacaaggt agaagaagcc gaaatcgata caaagcttgc aaagttcctc tacgcaaaca
     1861 ggggcgaata ttggttctgc gatccaaaag atccttcaaa gagatacacg cttcctgaaa
     1921 gcttgctcgg agatgccgcc aaattcatga agcagaattc cgaaattgaa acaaagcttt
     1981 ttgacgaaaa agtaatcggc gttaagcttc ctatcaaggt tgagcttaaa gtaaaggaat
     2041 cagctcccgg agttaaagga gacagcgcta agggcggaat gaagcaagcc actcttgaga
     2101 ctggcgcggt tataaacgtt cccctcttcg ttaacgaagg cgacattcta agaataaaca
     2161 cggaaaccgg agaatatgta gagcgcgtta attaacacgc tgagttgaga gtcgaatgtc
     2221 cataaagttt ataaagtaaa aacctcccct tttcggggag gttttatttt atccatcttc
     2281 tgctttctgt cctctatttt cttattatct agaaacgtaa ggaagcaaac ctaggaatct
     2341 ggctctcttc accgctgttg cggtctttct ctggtgtctg gagcatgtat ttgttctcca
     2401 tgacctcaaa atagaagagt gaggattcaa gaactttccg agagactcgg tgtccttgta
     2461 gtcgatcgac ttcacgttat ttacacaaaa atgacactgt ttcattgatt agtttttagt
     2521 ttttagtgat tagtttatgg attttagttt gttgttcgta agattactac cagctattta
     2581 ctattatcta ccaactagaa aggtatatcg tccggattaa tttcctcctc cggatagtct
     2641 atctgctgac ccacagaatt tgattccgcc ggagcactgt tctttgacgg atatgtgctt
     2701 ccgcccacag aaccgcccgc ctttggacca aattgaacct tctccgcgat tatttctgtt
     2761 ctgtttttct taacgccatc cttgtcgtcc cagtttcttg tctggatcct gccctctacg
     2821 agaacggcct gacccttttt caaatattgc gcgcaagtct cggcctgtct tccgaacaca
     2881 gaaatattgt gaaattctgt cccctcctgc ttctttccat ctttatcctt ccaaactcta
     2941 tttgtggcga cactgaaaga tgtgactttc tgccctgaag ggagtgctct catttcaggg
     3001 tctttcgtca tgttgccgat tataatagct ttgtttaggt acatatttga tgaaaattaa
     3061 ttttttatgc tgttttgtct aggaggttgt cgatctcttt gtccagttcc ttttcagaaa
     3121 cggttttctt tggtttcttc cctactccca gcggcttttt agcctcggcg gctccatttg
     3181 aatctcctcc ctctttcgtg agattgatta ttaaatatct aatcaatgac tggttgtttt
     3241 tcaatcctgc gtcaagcttt gacaccgcat ctgccgaaag attaaacttc atccatccga
     3301 agaaagcgtc gtcatattta gctcttctgc cgcctgttat gtcgtgagac atctcataag
     3361 cgagttttct ctccttagga gcctcttcga acaaaacaag gcctccattt gactcaacta
     3421 aagccctgat agcgtttacc tcgtcggcaa gcttctccgg agcgatgatt ggaatcaaaa
     3481 gataccccaa ttcgtatacg tttttatcgc tactatccat tttttattta ttaactgcta
     3541 actgctttag atacttcgta tatttacacc aacggggtca tactatcaga aaaaaccgaa
     3601 tatagcaata ataacaaaaa ccgccccatt taaggggctg ggcatatttc acccccagta
     3661 ggaaagttcg aattaccaag gtggattatt taccaagatc tcccggaacc cgccttcggc
     3721 tttatgcgtt gagatatacc ttaaaattag aaacttccta cggggcaaaa taaaaaacac
     3781 cgagcaaggc ccggtgtttt ttattttgga ctaggcgagt gttaccatct ctgccttagg
     3841 tccctttggg ctctcgccca tttcaaaggt aacctcctgt ccaaccttaa gtgaatcgaa
     3901 ggcaacgccg ttcaaatcct tagcgtggaa gaaaacgtcc ttggctccct cgattccgat
     3961 gaaaccaaat ccgttcttca aaagctttat tgttcctctc atttgaaaaa ct
//
LOCUS       DAOTEX010000064         3822 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3118
            NODE_17743_length_3822_cov_3.362623, whole genome shotgun sequence.
ACCESSION   DAOTEX010000064 DAOTEX010000000
VERSION     DAOTEX010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868142
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3822)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3822)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 883
            CDSs (total)                      :: 833
            Genes (coding)                    :: 829
            CDSs (with protein)               :: 829
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3822
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17743_length_3822_cov_3.362623"
                     /isolate="AMR_MDS_3118"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..875
                     /locus_tag="P5056_03830"
     CDS             <1..875
                     /locus_tag="P5056_03830"
                     /inference="COORDINATES: protein motif:HMM:NF019869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRY62871.1"
                     /translation="KKELIGNKAEKYFMPVDVYVGGAEHATRHLIYARFWHKFLYDMG
                     VVSHDEPFMKLHSVGLIMAEDGKKMSKRWGNVINPDDVVAIYGADTLRVYEMFMGPFN
                     QAIAWKTDNMIGSRRFLERVWRLQAKVSKKEKVSEEMESNIQELIKKVSGDIDSFSFN
                     TAISAMMIFINSLDEHATIPQSAYERLVLLLSPFAPHMTEEIWHLLGHKKFIYKEEWP
                     KFDASKIKKSKINLTLQVNGKVRDNVMVSADISEEDVKKIALENDAVKRNIEGKEIKK
                     VIYVKGRLVNIVAV"
     gene            complement(1081..1521)
                     /locus_tag="P5056_03835"
     CDS             complement(1081..1521)
                     /locus_tag="P5056_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62872.1"
                     /translation="MYVRSGLEKHLDKTWAKHGALSRIRGNIGWSLVRTEVLPNSLDK
                     NLNEQMELLTRSDRIPSVGTLINAIIAYRLLNGRGIFMETFARCIDSTEELYRFNHCY
                     HVSRTRTGELYSHHFSAAGREPRLGLATRKKTIPELLSEGIPIS"
     gene            complement(1852..2505)
                     /locus_tag="P5056_03840"
     CDS             complement(1852..2505)
                     /locus_tag="P5056_03840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62873.1"
                     /translation="MANRNEECRYTKTAHYQIWAKKLMGGNFLGVVCAERILGRPLSR
                     YERGILRDMPFSVEMLKACKHTHVLIAVPYITINEMYDDTRLHKLFCKTTSNWWRSEN
                     FAKEKMRVGWYLVRKNEGPDSVGHDFRAHKIMEAEGRIGGPDEVIAPANIVIYTMALR
                     KCYLGEIPFPQGVYTRDSAICPKEQVIVCSDFFTDQIEIGLAPICTTLGCATMKKVY"
     gene            2970..>3822
                     /locus_tag="P5056_03845"
     CDS             2970..>3822
                     /locus_tag="P5056_03845"
                     /inference="COORDINATES: protein motif:HMM:NF016450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HRY62874.1"
                     /translation="MEIKKKYFSQYKKPMMHLPDLVEMQKDSFKWIVEKGLAEILKEF
                     SPIRDYTGRKFELEFTGYELGLPKMNEHEAKANRATLNSTLKVKVKLKNKILNYEKEQ
                     EIFLTDFPAMTPHGTFVINGVERVIVPQLARSFGVFFTAEDIKGKKHFGAKIIPVRGA
                     WIEIETEADGVLYVRIDRRRKFPVSSLLRMLADGKTDAEIMKLFPKEASADEVVSGEK
                     SLDELNYVKNSFAKDTAKTTDESFIEIYKRLRDSDIATAASAREFLTDLFSPERYDLS
                     LVGRFRFN"
BASE COUNT         1071 a          825 c          946 g          980 t
ORIGIN      
        1 acaagaaaga acttatcggc aacaaggctg agaaatattt tatgcctgtt gatgtatatg
       61 taggcggagc cgagcacgcc acaagacacc ttatatatgc gaggttctgg cacaaattcc
      121 tatatgatat gggcgtcgtt tctcacgatg agccatttat gaagctgcac agcgtcgggc
      181 ttattatggc tgaggatgga aagaaaatga gcaagaggtg ggggaatgtt ataaatccgg
      241 acgacgttgt tgctatatat ggggcagata ctctgcgcgt atacgaaatg tttatgggcc
      301 cgttcaatca ggcaatagca tggaagaccg acaacatgat cggttccaga agattcttgg
      361 aaagggtttg gagattgcag gcgaaagtaa gcaagaaaga aaaagtttcc gaggagatgg
      421 agtccaacat ccaagagctt ataaagaagg tttcgggaga catagattct ttcagcttca
      481 ataccgccat aagcgcaatg atgatcttta tcaattcttt agatgagcac gcgacaatcc
      541 cacaatcggc ctatgaaaga ttagtattgc ttctttctcc ttttgctccg cacatgacag
      601 aggaaatatg gcatctcttg gggcacaaaa agttcatata caaggaagaa tggccaaagt
      661 ttgatgcctc caagataaaa aagagcaaaa ttaatctgac gcttcaggtt aatggtaagg
      721 tcagggacaa cgtaatggtt tcggccgata tttctgagga ggatgtaaaa aagatcgccc
      781 ttgaaaacga tgcggttaaa agaaatattg aggggaaaga aataaaaaag gtgatatatg
      841 tgaaggggag actggtgaat atagtggctg tttagagagt agatagtaga tagtagagag
      901 tagagaatag ttaaaataaa taccccggta ggaaagttcg gattgattat tccttaatgc
      961 gagtgttatc ttctcggcag gcgcctacgg cttctcgaaa tggttaaatg gtgatttccg
     1021 caaaacttcc tacggggcaa gaaaaaggcc acctgagagg gtggctttgt tgctgcgtga
     1081 ctacgaaatc gggatgcctt cggagagaag ttcgggaatc gttttcttcc tggtagcgag
     1141 accgaggcgg ggctctctgc cggctgcgct gaagtggtga gagtaaagtt ccccggtgcg
     1201 cgttctgctt acgtggtagc agtgattgaa acgataaagt tcctcagttg aatcaatgca
     1261 ccgcgcgaac gtttccatga agatccctct gccatttaaa agtcgatagg cgatgatagc
     1321 gtttatcaga gtcccgactg aggggatacg atcgctccgg gttaaaagct ccatctgctc
     1381 attcaggttt ttgtcgaggc tgttcggcag aacttccgtc ctgacgaggc tccacccgat
     1441 attgccccga atccttgaga gcgctccatg tttggcccaa gttttgtcta aatgtttttc
     1501 aagtccagat cttacataca tgtcgttggg ggaaagccca actttgacag caagagtatg
     1561 gtcacgtcgt gcatcctcga gcatcttttc ggtgaacggc acctgtttca gggcgtcccg
     1621 gttttccgaa ctcaggcgaa acttcaagcg gtccagtgct tcttctaacc cccagaaagc
     1681 tctgggaccc atgatactac tcgccacgat ggacgataca ccccttgtta agtctagtgt
     1741 cataatccct cgcacccctt tcaaagaacg ctatgtttga gtgttttata ccatatatcg
     1801 tgtgaaagta aaagggccgc tctttcgagc ggcccacatg ctatcttctt tctagtaaac
     1861 cttcttcatc gtcgcacagc cgagggtcgt gcagataggc gcgagaccaa tctcgatctg
     1921 gtccgtgaaa aagtcggaac agacgatcac ttgttccttg ggacaaatcg ccgaatcccg
     1981 cgtgtagact ccttgaggga aggggatttc gcccaggtag cacttgcgca gggccatcgt
     2041 gtagatgacg atatttgcgg gagcgatgac ttcatcgggc ccgccaattc taccctccgc
     2101 ctccatgatc ttgtgagccc taaagtcgtg ccccacggag tcggggcctt catttttgcg
     2161 aacgagatac cagccgaccc gcatcttctc tttggcgaaa ttttccgacc gccaccagtt
     2221 gctggtcgtc ttgcagaaga gcttgtggag ccgggtgtca tcgtacattt cgttgatggt
     2281 gatgtagggc actgcgataa ggacgtgcgt gtgcttgcag gccttgagca tctcgacaga
     2341 gaacggcatg tcccgaagga taccccgctc ataccgggaa agcggacgac cgaggatgcg
     2401 ttcggcacaa acgacgccga ggaagttccc gcccatcagc ttcttggccc atatctggta
     2461 gtgcgcggtc ttggtatacc tacactcttc attcctgttc gccatagaaa ttgctccttt
     2521 cagccgatct ctcggtccag gactcctaac ggccggagtg tttagacttt tggtttacta
     2581 tttgatttaa agtgctttcc gtgttgaacc atatcacagg ccgtatattg tgtcaaattg
     2641 aacagttatt ccacaggtca atctttttga ctattgacaa aaaaccagta tttttctata
     2701 ctacctttac ctgttgcaag attgagtttt tcttgaatat accacaaatt aatagcagtt
     2761 tagcgctaag aataatcttg ccaacaaaat tcttaacaat aagcgccgct aattagcgga
     2821 aatggaccaa cttgtataaa gttgggtttt tccttgttga agttggattg tcacgcctcg
     2881 gcttatgatg cctcggctcg caatgacaaa agaggatata gaataattta ggagtttatt
     2941 aaaaagaatt atcaataata ttcagggtca tggaaataaa aaagaaatat ttctcccaat
     3001 acaagaagcc gatgatgcac ctaccggatt tggtagagat gcagaaggac tcttttaaat
     3061 ggattgtgga gaagggcctc gcggaaattc tcaaggagtt ttctcctatc agagactata
     3121 cgggcagaaa gtttgaattg gaattcaccg gctatgagct ggggcttcct aagatgaacg
     3181 agcacgaggc aaaggcaaac cgagccacgc ttaacagcac gctcaaggta aaggttaagc
     3241 ttaagaataa gattcttaat tatgagaagg agcaagaaat ctttctcact gatttccctg
     3301 cgatgacccc gcacggaact tttgttatta acggagtaga aagagttatc gttccacagc
     3361 tcgccagaag cttcggtgta ttcttcaccg cagaagatat aaagggcaaa aagcactttg
     3421 gagccaaaat aatacctgta agaggagcat ggattgagat cgagacagag gctgacggtg
     3481 ttctatacgt aagaatagat agaagaagga aattccctgt ttcctcactc cttagaatgc
     3541 tcgccgacgg taagacagac gcggagataa tgaaattgtt ccctaaagag gcctcagcag
     3601 acgaggttgt ctccggagag aagagcctcg acgaattgaa ctacgttaaa aattcttttg
     3661 cgaaagacac agctaagaca acagacgaat ctttcattga aatctacaag agacttagag
     3721 atagcgatat cgccacagcg gcttccgcca gagagttcct cacagacctc tttagcccag
     3781 agagatacga tctttcactc gtcggaagat ttaggttcaa ca
//
LOCUS       DAOTEX010000065         3757 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3118
            NODE_18222_length_3757_cov_4.993247, whole genome shotgun sequence.
ACCESSION   DAOTEX010000065 DAOTEX010000000
VERSION     DAOTEX010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868142
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3757)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3757)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 883
            CDSs (total)                      :: 833
            Genes (coding)                    :: 829
            CDSs (with protein)               :: 829
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3757
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18222_length_3757_cov_4.993247"
                     /isolate="AMR_MDS_3118"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /locus_tag="P5056_03850"
     CDS             <1..312
                     /locus_tag="P5056_03850"
                     /inference="COORDINATES: protein motif:HMM:NF014130.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-binding factor A"
                     /protein_id="HRY62875.1"
                     /translation="QKLAAEFIEKNSNGKSMITVTAIDLSPDMKEAKVLFTVLPSSAE
                     DAAEDFLKRQRSDFRDYVKKVWRMRTIPFFDFELDKGERHRQRIDELLSQAKQADELK
                     K"
     gene            complement(345..416)
                     /locus_tag="P5056_03855"
     tRNA            complement(345..416)
                     /locus_tag="P5056_03855"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(382..384),aa:Cys,seq:gca)
     gene            complement(427..510)
                     /locus_tag="P5056_03860"
     tRNA            complement(427..510)
                     /locus_tag="P5056_03860"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(474..476),aa:Leu,seq:taa)
     gene            complement(498..1004)
                     /locus_tag="P5056_03865"
     CDS             complement(498..1004)
                     /locus_tag="P5056_03865"
                     /inference="COORDINATES: protein motif:HMM:NF013964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HRY62876.1"
                     /translation="MFIQERRKRKWSLGQLSDAVKNSKSIRQVIDKLGLVPAGGNYEQ
                     LKKYIKQEGLNTGHFTGKVWNKGLRGIGKPRVPLFKILTKDSPFQSFKLKKRLFAEGI
                     KSRNCELCGWAKTAMDGRLPLELDHINGNKFDNRIENLRILCPNCHSLQPTHRGRNQK
                     RYKNKPEW"
     gene            complement(1232..1304)
                     /locus_tag="P5056_03870"
     tRNA            complement(1232..1304)
                     /locus_tag="P5056_03870"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1269..1271),aa:Lys,seq:ctt)
     gene            complement(1362..1823)
                     /locus_tag="P5056_03875"
     CDS             complement(1362..1823)
                     /locus_tag="P5056_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016482193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HRY62877.1"
                     /translation="MATVRKFEDLLCWNKARELSREVYKAFRNINDRGLKDQIERAAV
                     SVMSNIAEGFERGTKQEFLNYLFIAKGSAGEVRSQLYVALDVGYLNIETFKYLNSLAL
                     ENSRLIHSFTEKVKGGASSGTQFKKIDKQDPLEEIVKDRAPELYERFYGKK"
     gene            complement(1834..2739)
                     /locus_tag="P5056_03880"
     CDS             complement(1834..2739)
                     /locus_tag="P5056_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008442768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRY62878.1"
                     /translation="MKKKIKVVVTGGAGFIGSNLTDALVEEGFDVHVIDNLVGGKKEN
                     VNKKAKLRIADITDLNKIKPIFKGAEYVFHLAALPRVQYSIEHPEETNEANITGTLNV
                     LIAAKDAKVKKLVYSASSSAYGDQKIMPLREDMRANPKSPYGLQKYVGELYSRLFYDV
                     YGLSTVCLRYFNVYGPRQSDEGAYALVIAKFLKQKAEGKPMTITGSGKQTRDFTHVSD
                     VARANILAMKSNKVGKGEVLNIGCGSNQSVNKIAKLIGGETVHIPARLEPKDTLADNR
                     LAKKLIGWAPKVKIEEGIRRLKENE"
     gene            complement(2795..3199)
                     /locus_tag="P5056_03885"
     CDS             complement(2795..3199)
                     /locus_tag="P5056_03885"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HRY62879.1"
                     /translation="MEDKSMKSEASSAIIVRDIDRKVFLARRSLRKQYSPGEWEMVGG
                     VMEDGETAEDCVAREIREELKCELASLKFFRSYKFAEKEFHVFEVKLDSEPRPNVQDF
                     EEWGWFDENSIKEMNFALNCKERLLDYFEETH"
     gene            complement(3189..>3757)
                     /locus_tag="P5056_03890"
     CDS             complement(3189..>3757)
                     /locus_tag="P5056_03890"
                     /inference="COORDINATES: protein motif:HMM:NF019155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SEC-C metal-binding domain-containing protein"
                     /protein_id="HRY62880.1"
                     /translation="YDDVINIQIKTIYEKRRKILLGDKEYLQNFLEEISLGDEETISS
                     ANKIKEELGEDNFYSAFRILSLQVVDMIWTDHLDTMDHMRSSVRLRAYGQRDPLVEYK
                     KEGIKLFREIENTMADSIVKLLSTLRGNNIQMGATEEFKEVHESAVTIGNSEDEDHKK
                     IEEDKITVGRNDPCVCGSGKKFKKCCGR"
BASE COUNT          971 a          948 c          638 g         1200 t
ORIGIN      
        1 caaaagctcg ctgcggaatt catagagaaa aactcaaacg gcaaatcaat gatcactgtc
       61 acagcgattg atctttcacc tgatatgaaa gaagctaagg ttctgtttac cgtgcttcca
      121 agcagtgcgg aggacgccgc ggaggatttt ctcaaaagac agaggagcga ttttcgtgac
      181 tatgtgaaaa aggtttggag gatgaggaca atccccttct ttgattttga attagataag
      241 ggagaaagac acagacagcg aatagacgaa cttttaagcc aagcaaaaca agcggacgaa
      301 ttaaaaaagt aaaaaacacc cactatcggg tgttttttaa tttaaggcct gggggagaat
      361 cgaactcccg catagaggtt ttgcagacct ctgccttacc acttggctac caggccatct
      421 tctgtgtgcc cgaggtggga gtcgaaccca caccagcttg cgccgacaac attttaagtg
      481 ttgcgtgtat accaatttca ccactcgggc ttatttttgt atcttttctg attcctcccc
      541 ctgtgagtag gttgtaaact atgacaatta ggacaaagta tcctcaaatt ttctatccta
      601 ttgtcaaact tattgccatt aatgtgatct agctcgagcg gcaatctacc atccatggca
      661 gtttttgccc agccgcataa ctcacaatta cgagacttaa tcccctccgc aaacaacctc
      721 ttctttagct taaaactttg aaaaggactg tcctttgtaa gtattttaaa aagaggaact
      781 cgaggcttac ctatacccct caaacccttg ttccaaactt taccagtaaa atgtcccgta
      841 ttcaagcctt cttgtttaat atattttttt agctgttcat aatttcctcc tgcgggcaca
      901 agaccgagtt tgtctataac ctgtcttatg cttttagaat tcttaaccgc atctgataac
      961 tgtcccaaag accacttacg ttttcttcgt tcctgtataa acattattaa atcatagcat
     1021 acggaacacg aataagtaaa tataaacgta atcgtgttcc gttacaaaac aaaaaacatt
     1081 aaggcgctgg tgcgcgcaca ccggtacctc gactgaatct tttgtttcgc cttgctcaca
     1141 aaaactgctc aatcgggctt cggatcctta aacaaagcat ttaagatgct ttgttttggg
     1201 cacacaaaaa tccgctttcg cggattattt gtgcgcccag caggattcga acctgcgacc
     1261 attcgcttaa gaggcgactg ctctaccaac tgagctatgg gcgctcaatt tgtgaatata
     1321 aaataacacc tttaaggaaa attttcaagg ccgggcgttc cctacttctt cccgtaaaat
     1381 ctttcataca attccggcgc acggtctttt actatctctt ccaacgggtc ttgcttatct
     1441 attttcttaa actgtgtccc cgagctagcg ccgcccttca ctttttccgt aaaactgtga
     1501 ataagtctcg agttttccaa ggctaaagaa tttaaatatt taaatgtttc aatatttaaa
     1561 tagccaacat cgagtgcaac ataaagttgt gaacgcacct ctcctgctga accttttgcg
     1621 ataaataaat aatttaaaaa ctcctgtttg gtgcctctct caaacccctc agcaatatta
     1681 ctcatcaccg aaacggccgc acgttctatc tgatctttca gtccacgatc gttaatgttc
     1741 cgaaacgcct tatatacctc gcggctgagc tcgcgcgcct tattccaaca aagcaaatct
     1801 tcaaattttc ggaccgtcgc catatctcaa cgtttattca ttttccttca gcctccttat
     1861 tccctcctca atcttaacct ttggtgccca gccgatcagt ttctttgcta gtctgttatc
     1921 cgcaagtgta tccttgggct caagtctggc cggaatatga acagtttctc cgcctatcaa
     1981 tttcgcgatc ttgtttacag attgattgct tccacaccca atatttagca cctccccttt
     2041 tccaacctta ttgctcttca tcgcgagaat attggcacgg gcgacatcac tgacgtgggt
     2101 aaaatccctc gtctgtttcc cgctgccggt tatggtcatc ggtttacctt ccgctttttg
     2161 tttcaagaac ttggcaataa ccagagcata agccccctca tcgctctgtc ttggcccata
     2221 gacgttaaaa tatctaaggc ataccgtcga gagaccatat acatcataga acagtctgct
     2281 gtaaagctcg ccgacatatt tttgtaggcc ataagggctt ttcggattag cccgcatatc
     2341 ttctcggagc ggcattattt tctggtcacc ataagcggaa cttgatgccg aatacacgag
     2401 tttcttgacc ttggcatcct tggccgcgat caatacgttc aatgttccgg ttatgtttgc
     2461 ctcgttggtc tcctccgggt gttcgatcga atactgaact ctcggcaatg cggccaagtg
     2521 aaaaacatac tcagctccct taaaaatagg ttttatttta tttagatctg ttatgtcggc
     2581 tatgcggagt tttgcttttt tgtttacgtt ttcctttttt cctccaacca gattgtcaat
     2641 tacatggaca tcaaacccct cttctaccag ggcatctgtc aaatttgaac cgataaatcc
     2701 cgcgcccccg gtaaccacaa cctttatttt tttcttcata tctttttatt tgcaacggca
     2761 cttcctgatc ttattcctgt atccgcagga attattagtg tgtttcttca aaataatcca
     2821 ataatcgttc tttgcagttt aacgcgaagt tcatttcttt gattgaattt tcatcgaacc
     2881 atccccattc ttcaaaatcc tgtacattgg gtcttggctc tgaatccaat ttcacctcga
     2941 agacatgaaa ttctttttca gcaaatttat aactcctaaa aaatttcagc gacgctagct
     3001 cacatttcag ttcctctcgt atttcccttg ccacacaatc ttctgcggtt tctccatcct
     3061 ccataacacc accgaccatc tcccattctc cgggagaata ttgttttctc aagcttcttc
     3121 tcgccagaaa aacctttcta tctatatctc taacaataat tgcgctactt gcttctgatt
     3181 tcatagattt atcttccaca acacttctta aactttttcc cgcttccgca tacacaggga
     3241 tcatttctgc ccacagttat tttatcttcc tctatctttt tgtggtcctc gtcttcggaa
     3301 ttacctattg ttacagccga ctcatggacc tccttaaact cttccgtggc acccatttgg
     3361 atattattac ccctcagagt tgataaaagt ttcacaatgc tatcggccat cgtattctct
     3421 atctctctga acagctttat tccctctttt ttatattcca ccaacggatc gcgttgaccg
     3481 tacgccctca atctgacgct ggacctcata tggtccatgg tatccaggtg gtcagtccat
     3541 atcatgtcta caacctgaag tgaaagtatc ctgaatgcgg aataaaaatt atcctctccg
     3601 agctcttctt ttattttatt cgcagaagag atagtctctt cgtcgcccaa agagatctcc
     3661 tctagaaaat tttgtagata ttctttatct ccgagcaata tttttctcct cttttcatag
     3721 atagtcttta tctggatatt tataacatcg tcatact
//
LOCUS       DAOTEX010000066         3744 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3118
            NODE_18330_length_3744_cov_4.550556, whole genome shotgun sequence.
ACCESSION   DAOTEX010000066 DAOTEX010000000
VERSION     DAOTEX010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868142
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3744)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3744)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 883
            CDSs (total)                      :: 833
            Genes (coding)                    :: 829
            CDSs (with protein)               :: 829
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3744
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18330_length_3744_cov_4.550556"
                     /isolate="AMR_MDS_3118"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            192..569
                     /locus_tag="P5056_03895"
     CDS             192..569
                     /locus_tag="P5056_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62881.1"
                     /translation="MYLTERGRLVFTMIIATAASFLAGVNYIYDPKYPILTGLIVGAV
                     TALGLTISFSIYMIIGLAVGCGETKKNMPPRVPVSKQETNKGGCGKPECANCTSCHCK
                     PSPSGKNEDNENCIGCGYRHDHL"
     gene            671..823
                     /locus_tag="P5056_03900"
     CDS             671..823
                     /locus_tag="P5056_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62882.1"
                     /translation="MRVNDVVVNCRRRSEQRRDEIWWEQFYRDLGLAATGLLIWGFLF
                     CAMLAR"
     gene            855..1058
                     /locus_tag="P5056_03905"
     CDS             855..1058
                     /locus_tag="P5056_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62883.1"
                     /translation="MLFNTNALGTPDDNRKKKRTKKQKRGKEAQKQTPIQKIGLGIII
                     AACSLGIPVAIAFASYTAPIVLK"
     gene            complement(1282..1710)
                     /gene="rplK"
                     /locus_tag="P5056_03910"
     CDS             complement(1282..1710)
                     /gene="rplK"
                     /locus_tag="P5056_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012858558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HRY62884.1"
                     /translation="MAEKKVVKKIKLQIPAGKAVPTPPVGPALGQAGVNIGEFITKFN
                     AATAPMMGSIIPVEISVYDNRSFDFVLKTPPASDLIAKAAKIEKGSGSNVKTKAGKIT
                     RAQLREIAEKKMADLTAASVEAAMNTIAGSARSMGVEIVD"
     gene            complement(1786..2328)
                     /gene="nusG"
                     /locus_tag="P5056_03915"
     CDS             complement(1786..2328)
                     /gene="nusG"
                     /locus_tag="P5056_03915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HRY62885.1"
                     /translation="MKQELNTERNWYAVHTYAGYENAVARNLKQRIESLGMEDKIFNV
                     IVPVEKKVKIKGGKRVTIEEKIYPGYVLVDMIVTDDSWYVVRNTPRVTGFVGAGVKPV
                     PLDKTELDTLFKKMGTDTAKHQIDLIVGDLVRIVDGPFKDFEGKVSGVDTERGKVKVF
                     VSMFGRETAVELDFLQVNKV"
     gene            complement(2493..2681)
                     /gene="secE"
                     /locus_tag="P5056_03920"
     CDS             complement(2493..2681)
                     /gene="secE"
                     /locus_tag="P5056_03920"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HRY62886.1"
                     /translation="MLNSFIAYVKETRAELKHVSWPTNVQTINFTIAVVVISVATSIY
                     LAFFDSVFISLLQKVILG"
     gene            complement(2793..3632)
                     /locus_tag="P5056_03925"
     CDS             complement(2793..3632)
                     /locus_tag="P5056_03925"
                     /inference="COORDINATES: protein motif:HMM:NF014342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HRY62887.1"
                     /translation="MREEPKKILVLYHADCSDGFGAAYAAWKKFGDSADYLPILYKTS
                     PPDVRGKIVYLLDYGYPKDVTDAIARNAERFVMIDHHITNEDTVKKASEYVFENSHSA
                     AVLSWKYFHPDIKVPTLFEYIEDKDLWKFSLPFSREVSRWFAGLPADFVAWDGVVSDF
                     EDEKTRNTFIAEGSRILAQVNKVISDLVSRAKPIVFEGYKTLIIETPVFHSEVGNALA
                     QKMPPIGIVWVEKNGKYSVSLRSDGTVDVSKMAEKYGGGGHKSASGFVLDTKEELNSL
                     FGL"
BASE COUNT         1012 a          977 c          746 g         1009 t
ORIGIN      
        1 tgttattgtt aatgttctat catgtcgggt gtttccaagc tattgaacca taacgacggg
       61 gttgacaaaa taacctaata ggtatataat ataaaaagtt aagattggta tttgaaaaca
      121 taaacaaaag gaggatttac accgccttgt cccctaacga aaacacgaga ctttcccaaa
      181 ggagggaaac tatgtacctt acggagcgag gcaggcttgt cttcacgatg atcatcgcga
      241 ccgccgcgag cttcctcgcc ggcgtcaact acatctacga ccccaagtac cccatcctga
      301 ccggcctcat cgtcggtgcc gtcacggccc tcggcctcac gatctcgttc agcatctaca
      361 tgatcatcgg cctcgcagtt ggctgcgggg aaacgaagaa gaacatgccg ccccgtgtcc
      421 ccgtcagcaa gcaagagacg aacaagggag gctgcggcaa gcccgagtgt gcgaactgca
      481 cctcgtgcca ctgcaagccc tccccctcag gcaaaaacga ggataacgag aactgcatcg
      541 gctgtggcta ccgccacgac catctgtgat tggaggacaa taaggtgatc tccctcgagt
      601 ggaagctgac catcctcgcc gtcatcgtca tcgccgccct cgtcctaatc ggatagaaag
      661 gagtgcgaac atgagagtga atgacgtcgt cgtgaactgc agaaggcgta gcgaacagag
      721 gcgggacgag atctggtggg aacaattcta ccgcgacctc ggactcgccg ctaccggcct
      781 cctcatctgg ggcttcctct tctgcgccat gctcgcgcgg taactccgca gtgcggagag
      841 aaagagagac taaaatgctc ttcaacacga acgcgctcgg caccccggac gacaaccgca
      901 agaagaagcg gaccaagaag cagaagcggg gcaaggaggc ccaaaagcag accccgatcc
      961 agaagatcgg cctcggcatc atcatcgccg cctgctccct cggcatcccg gtcgcgattg
     1021 ccttcgcgag ctacaccgcc cccatcgtcc tcaagtagtc gcgttagcaa taacgcaaaa
     1081 gcccccggct aataaccggg ggtattttta ccccgtgaaa tcaaattatt aacaccacca
     1141 cgatactcca aacagaacga aaaatcgtcg ttcataataa aaaacaaatc tgcatctatt
     1201 tcactggggt tttacccaaa caaaaccact tggaaaccgg gtttccaagt ggtttttagc
     1261 tttttaatta taacctttgc cttaatctac aatctcaact cccattgatc tggcggagcc
     1321 ggcaatggtg ttcatcgcgg cctctacaga tgcggcggta agatcagcca ttttcttttc
     1381 ggcgatctct cgaagctgtg ctcttgtgat ctttccggcc tttgtcttta cgtttgaacc
     1441 ggatcctttc tcgatctttg cggccttggc gataaggtct gaagcgggag gggtcttcaa
     1501 tacgaagtca aagcttctgt tgtcgtaaac ggaaatctca acaggaataa tgcttcccat
     1561 catcggagcc gtagcggcgt tgaatttcgt tatgaattct ccgatattta caccagcctg
     1621 accaagagca ggtccaaccg gaggcgtagg aacggccttc ccggcgggaa tctgcaattt
     1681 tattttctta acaacttttt tctctgccat ataaaattaa ttatcaatta ctaattacga
     1741 attacgaatt ataaatagaa gcttttataa aacgcaatta gttttctaaa ctttatttac
     1801 ctgcaagaag tccagctcta cggctgtctc tcttccaaac atagagacga acaccttcac
     1861 ctttcccctc tctgtatcta caccgcttac cttgccttca aaatccttga acggaccgtc
     1921 gactatcctt accaaatctc cgacgataag gtctatctga tgctttgccg tatctgtgcc
     1981 catcttcttg aagagtgtgt ccaattctgt tttatccaaa ggaacaggct ttactcccgc
     2041 gccgacgaat cctgtaaccc taggggtgtt tctaacaaca taccaagagt catcggtaac
     2101 tatcatatca acgagcacat aaccgggata tattttttcc tcgattgtga cccttttccc
     2161 tccttttatc tttacctttt tctcgacagg aacaataaca ttgaatattt tgtcctccat
     2221 tcccagggac tcgatacgtt gtttcaagtt tcttgcgacg gcattttcat aaccggcgta
     2281 tgtgtgaacg gcgtaccaat ttctttctgt gttcagttct tgtttcatcc tcctaaattt
     2341 taaagcaatt gaataataac ttcttcaact ggtttcgccc ccatccagtt tttaaaaatt
     2401 tctccctctc aaaagcgtca tacttattac taaacgcttc gtaaaaaatt aacttaaatg
     2461 gacccttatc cttcgtcgac tttgagccac cactaaccca aaattacctt ctgaagcagg
     2521 gagatgaata cgctgtcaaa aaatgccaaa tatatagaag tagcaaccga aataacgaca
     2581 acggcgattg tgaaatttat agtctgaaca ttagtaggcc agcttacatg ctttaactca
     2641 gccctagtct ccttaacata tgcgataaaa gaatttaaca tgtttttctc taattaaacc
     2701 tattttaaac caccctcgcg ggtggtactt aatataatac tcagaacaga aaaaatgtca
     2761 acgagttcca taaaaacgat attttatcct tcttaaagac cgaaaaggct atttaattcc
     2821 tctttggtat ccaaaacaaa tcccgaggcc gacttgtggc cccctcctcc atatttttca
     2881 gccattttag aaacgtctac tgtgccgtca gacctcaaag ataccgaata tttaccgttc
     2941 ttctctaccc aaactatacc tatcggaggc atcttctgcg ccaaggcatt accaacttct
     3001 gaatgaaata cgggggtctc tataataaga gtcttgtacc cttcgaaaac tatcggcttt
     3061 gcgcgcgaga caaggtcgga aatcaccttg ttcacctggg ccaatattct actcccctct
     3121 gctataaaag tgtttcttgt tttttcatcc tcaaaatcag agacgacgcc gtcccacgcg
     3181 acaaaatctg cgggcagtcc ggcaaaccaa cgggaaactt ccctactgaa cggcagtgaa
     3241 aatttccaca gatctttgtc ttctatatat tcaaaaagtg tcggaacttt tatgtcagga
     3301 tgaaaatatt tccaagatag aactgcggcg gagtgcgaat tttcaaaaac atattcggaa
     3361 gccttcttca cggtatcttc attcgtaata tggtgatcta tcatcacaaa cctctcggca
     3421 tttcttgcga tagcgtctgt gacgtctttt ggatatccgt aatcgagcag ataaacgatc
     3481 ttgcccctaa cgtcaggtgg agatgtcttg taaagtatcg gcaaatagtc cgcactgtct
     3541 ccaaattttt tccaagcggc ataagcggcg ccaaaaccgt ccgagcagtc ggcatgataa
     3601 agaaccagga tttttttggg ctcctctctc aattttaatt agagctagac ttaaccttaa
     3661 tcttatttac tgcgctttcc tctcttttcg gaattgtgat cttgagtatc cccttcttgt
     3721 agtcggcatc cgccttgtcc atct
//
LOCUS       DAOTEX010000067         3664 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3118
            NODE_18910_length_3664_cov_4.652812, whole genome shotgun sequence.
ACCESSION   DAOTEX010000067 DAOTEX010000000
VERSION     DAOTEX010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868142
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3664)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3664)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 883
            CDSs (total)                      :: 833
            Genes (coding)                    :: 829
            CDSs (with protein)               :: 829
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3664
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18910_length_3664_cov_4.652812"
                     /isolate="AMR_MDS_3118"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1362)
                     /locus_tag="P5056_03930"
     CDS             complement(<1..1362)
                     /locus_tag="P5056_03930"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase N-terminal 7TM
                     domain-containing protein"
                     /protein_id="HRY62888.1"
                     /translation="MITTYSTIIWLSAALTGVFSLLIYLGSNKPSTKYFSLTAFWAFI
                     WTLGMSGYFTASTYDWILFWGRWNHFSSSILAAAFLYFCLVFPKEKLPPKWVTITILT
                     IETILVYLHMLTDLIIRDYYQTGSRVVDRGVHFGDWGILFHIFFVGFFVAGFIILIKN
                     FFRETDPAIKAQIKFLMFGVFAGVVPIAIINLVLGTLGNFQYYWLGAPLTLGWVSISG
                     YVIVKHKALTVRLLVAELAVVAMALVLFSNIFFAEIFKLGLYGRLGIFLVFSIFGYFF
                     IKSVTDAENQREWLSILSLELKDFNQNLEARVIAETKEAEKERAHIETVTDTITSGLV
                     EYGKDMTIQRINKTAERLLGIDRRQIIGQRIEPQDAGGKWSSLASIIYPDAKGCNTCD
                     VPVDKPIKRELQVTTLPILDGYFKMFRDVTREKIIAKSKSEFISVIAHNFKEPLSTVK
                     TTLE"
     gene            complement(1366..2781)
                     /locus_tag="P5056_03935"
     CDS             complement(1366..2781)
                     /locus_tag="P5056_03935"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HRY62889.1"
                     /translation="MAANKTTPNTNKNIALSTAGVLIMSVVLLFYVVVGEESFGILAR
                     SVVFAAFAMMGGIFLKNIVQNEDRKDELGALNKELEKANTILKEKVNERLAEIENSRV
                     HLETIIENLASGLVEYDNGGAVRRINRAAEEMLSTSRKDVMGIIPKDDSSARISLITY
                     PRLAKNVLEIDPELYGIEALGAEIHEITIPGETEDVLQVTSVPLVVADEKNGTLKIIR
                     DITKEKTVSRSKSGFITTAAHQLRTPLSVIKWAVNLLLEGDKGALKPEQIPELEKTNI
                     ANEKMIVLVNNLLDVARIEEGRYGFKFKEGDITPIIEMIVSRVLPKAEKKSISLSWTN
                     PFPPISKIFFDEEKIMLALSNIIGNAVAFTKEKGTVNIFAENDSDYVKITVEDSGVGI
                     PPEAQVHLFEKFFRAANAFQIRPDGSGLGLFVAHNIVKRHGGKIKIISPILGDLGTRV
                     EVYLPKDKEKIPRLEEGEFAP"
     gene            3137..3223
                     /locus_tag="P5056_03940"
     tRNA            3137..3223
                     /locus_tag="P5056_03940"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3171..3173,aa:Ser,seq:gct)
     gene            3371..>3664
                     /locus_tag="P5056_03945"
     CDS             3371..>3664
                     /locus_tag="P5056_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012504184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HRY62890.1"
                     /translation="MSTNERKTEDLVREVLKENKKIYFSQTGHTAIIEEQKSDNARIN
                     KLLQFASKKGGGVGKPEFVISFPNREVLIVIECKGDVRFHKSKTLNKYGDFAVD"
BASE COUNT          986 a          751 c          803 g         1124 t
ORIGIN      
        1 ctcgagggtc gtcttgacgg tagaaagagg ctctttgaag ttgtgagcga tgacagagat
       61 gaattcgctc tttgattttg cgattatttt ttctcttgtt acgtctcgga acattttgaa
      121 atatccgtct aagataggca gagttgtgac ctgcaattct cttttgatcg gcttgtctac
      181 cggcacatcg caagtattgc accccttggc gtccggataa attatgcttg caagcgagct
      241 ccacttgccg cctgcgtctt gcggttctat tctttggccg attatttgcc tcctatctat
      301 tccaagtaga cgttcggctg ttttatttat tctttggatc gtcatatcct tgccatattc
      361 caccaatcca ctcgtgatag tatctgtgac ggtctctatg tgagctcttt ccttttcagc
      421 ttcttttgtt tcggcgatga cccttgcctc caggttttga ttaaaatctt ttagttcaag
      481 agaaagtatg gatagccact ctcgctggtt ttccgcatcc gtgacgctct ttatgaagaa
      541 atatccgaat atcgagaaga cgaggaagat accgagccgt ccataaagac caagcttaaa
      601 tatctcggca aagaaaatgt ttgagaataa aacgagcgcc atcgctacga ccgcgagttc
      661 cgccaccaaa agtcggacgg tgagagcttt gtgttttaca atcacatatc cggagataga
      721 aacccagccg agggtgaggg gagcgcctaa ccaataatat tggaagttgc caagggtgcc
      781 caacactaga tttatgattg caatcggcac aactcccgca aaaactccga acatcaggaa
      841 ctttatttgt gccttaatag cgggatctgt ctctctgaaa aagtttttga taaggattat
      901 gaaacccgcg acgaaaaatc ctacgaaaaa gatatggaat agaatacccc aatcaccgaa
      961 gtgcactcct ctgtctacga ctcggcttcc tgtctggtaa taatctctga taatgagatc
     1021 ggtcagcata tgtaggtaga caagaattgt ctctatcgta agtatcgtga tggtgaccca
     1081 ttttggaggg agtttttctt ttgggaatac aaggcaaaag tataaaaagg cggccgcgag
     1141 tatggatgag gagaaatggt tccatcgtcc ccagaagagt atccagtcgt atgtgcttgc
     1201 cgtgaagtag ccgctcatcc caagtgtcca tatgaatgcc caaaaagctg ttaaagaaaa
     1261 gtattttgtg gagggcttgt tgcttccaag ataaatgaga agagaaaata cgcccgtaag
     1321 ggcggcggat aaccaaatta ttgttgaata tgtcgtgatc attttttatg gcgcaaattc
     1381 tccttcttcc agtctcggta tcttttcttt gtctttcggc agatatactt ctacgcgcgt
     1441 gccgaggtcg ccgagtatcg gagaaattat ttttatcttt ccaccgtgcc tttttactat
     1501 gttatgagct acgaataagc cgaggcccga gccgtccggc cttatctgga aggcgtttgc
     1561 cgctctgaag aatttttcaa aaagatgaac ttgcgcttcg ggcggtatcc ctactccgga
     1621 atcttcaacc gttattttca cataatcgct gtcgttttct gcaaatatat ttactgtgcc
     1681 tttttctttt gtaaaagcca cagcattgcc gattatgttt gatagagcga gcattatttt
     1741 ctcttcatca aaaaatattt ttgagatagg gggaaacgga tttgtccaag aaagagagat
     1801 agacttcttt tccgctttcg gcaaaactct ggaaacaatc atttctatta tgggcgtaat
     1861 gtctccttcc ttgaacttaa aaccgtaacg gccctcttct attctcgcaa cgtcaagaag
     1921 attgttgacg agaactatca ttttttcatt tgcgatattt gttttttcta attcagggat
     1981 ctgttccggc tttagcgctc ccttatcgcc ttcgagcaaa agatttaccg cccatttgat
     2041 gacagagagg ggggtgcgga gctggtgtgc ggccgtggta atgaacccac tctttgatct
     2101 tgagacggtt ttttcttttg taatatctcg gattatttta agtgtgccgt ttttctcatc
     2161 agcaacgaca agcggaacag aggtgacctg taagacgtcc tcggtttctc ccggtattgt
     2221 gatttcgtgt atttcagccc caagcgcttc tatgccatat agttcaggat ctatttccag
     2281 gacatttttg gcgagtctgg ggtatgtaat aagggaaatt ctcgcagacg agtcgtcttt
     2341 tgggattatt cccataacgt cttttcgtga agtagaaagc atttcctccg cggcgcgatt
     2401 tatccttctg acagcacccc cgttgtcata ttccactagt ccgctagcca aattttcaat
     2461 aattgtttca aggtgaactc gggaattttc aatttccgcc aatctttcat taactttttc
     2521 tttcaatatt gtgtttgcct tttcaagttc tttgttgagg gctccaagtt cgtcttttct
     2581 gtcctcattc tgaacaatgt tctttaggaa aatgccgccc atcatcgcaa atgccgcgaa
     2641 aacaacagac ctggccaata ttccaaaact ttcttctccg acaactacat agaacaaaag
     2701 aacgacgctc ataattaaaa ctcccgctgt agaaagcgcg atgtttttgt ttgtattggg
     2761 tgtggttttg ttcgccgcca tctatttaat attctaacat ttagaaaagg cgcaaggaat
     2821 ttttttatcc ggaatacttg tttattgagg tatttttgtc tatactaggc aaaatgttgg
     2881 agccgtgtca gagtgttgat ttgcgaagac ttggagcaaa tcaaggccgg agcgcgttag
     2941 cgcgaggcgg gctgaggatt gggttgggac ccaaccgaag gaccttttga gatattttat
     3001 gagcgtagcg aaatataaat atccaaaatg gtgtactgct cctgtaagga gcgcggaaat
     3061 tttcatcaga aaattatctg tgacctaacg tacctctttg ctaaaaagca gatgtagtaa
     3121 ataattgaat aatattggag ccgtgtcaga gtggtctaac gtacctcttt gctaaagagg
     3181 cggggtgtaa cagcccccgc tggttcgaat ccagccggct ccgctaagtg ttcaagttag
     3241 aaccacaatt gaatacagtc gacctcttgt ggataaagta aactattaga gaaaatgtcg
     3301 ttaaaaatgg acacattttc acgcttttga aaagctgttc aaaatttgaa ttttgggata
     3361 caatttctgt atgagtacaa acgaaagaaa aactgaagat ttagttcgcg aagtcttgaa
     3421 agagaataag aaaatttatt tctctcaaac tgggcatacc gcaataattg aagaacaaaa
     3481 atcagataac gcgcgaataa ataaactcct tcaatttgct tccaaaaaag gaggcggcgt
     3541 tggtaaaccc gaatttgtga ttagttttcc gaatagggag gttttaattg taattgaatg
     3601 taaaggagac gttagatttc acaagagtaa aactttaaat aagtatggcg attttgccgt
     3661 ggat
//
LOCUS       DAOTEX010000068         3641 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3118
            NODE_19098_length_3641_cov_4.739822, whole genome shotgun sequence.
ACCESSION   DAOTEX010000068 DAOTEX010000000
VERSION     DAOTEX010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868142
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 883
            CDSs (total)                      :: 833
            Genes (coding)                    :: 829
            CDSs (with protein)               :: 829
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3641
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19098_length_3641_cov_4.739822"
                     /isolate="AMR_MDS_3118"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..377)
                     /locus_tag="P5056_03950"
     CDS             complement(<1..377)
                     /locus_tag="P5056_03950"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRY62891.1"
                     /translation="MSVIFTTSWDDGDILDLKLSRLLDHYGVKGTFYVPQKYRENPLS
                     EDNIREIALRHEIGAHTLTHPDLHILRNEEKKKEICGSKEWLEKVINKEVEMFCYPFG
                     SYNKETADIVKECGFNGARTTEAG"
     gene            complement(378..1697)
                     /locus_tag="P5056_03955"
     CDS             complement(378..1697)
                     /locus_tag="P5056_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62892.1"
                     /translation="MNKSDKENIIEIDGWRVYDLPVKYTKEDYDGARAEIIKEAENTP
                     ELLALFEYGYIPAPGISDMDFWAVFPDDSEKMYIFPLPILSKKTRHIMKHQIILITEE
                     NYRKMLYLDPWTMNIWPNGHSQLYKKPGITRDLNFEKIKFSKEDYEILSALNVEEHLS
                     AIYSAIPLYAQKELPVRSVFEEIKSCIYIAREVSSITGKKIDQTFSEEFSELRSNWFN
                     LEQEQAIRRLIKIFYKGLLNGFESAFILSEWISKNSQIKKVNDVGIKNTNILSRSYLD
                     KNGKNVYLNTFRDRRLYSDTIKTPEQALRLSINSCKKIKINLGWRSKEVDSFIIFQPL
                     GMSAIILGLASESGLLSNSLKKDIFTNLDEVSVLNSEVFRKKMRMINDITEKYNKKQV
                     PQTNGKGWVAGNNRFGYSFEKEKIRRKFWTFWLKLKYWDAINENKDK"
     gene            complement(1694..2830)
                     /locus_tag="P5056_03960"
     CDS             complement(1694..2830)
                     /locus_tag="P5056_03960"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde reductase"
                     /protein_id="HRY62893.1"
                     /translation="MVTKQIEYIGKGTISKVREILDKTNSKKIFLVTGKDSYISSGAK
                     DYFESIKDDFELLQFNSFSSLPLCEDIKKGIDIYRSFDPDIIVAIGGGSVIDMAKSIN
                     ILSAQPQDADTYIIGDNKINNSGKALVAIPTTAGSGSEATSFAVVYKDGVKYSLQNEY
                     ILPKYSIIDPNLILSIPKNKLIPSALDALSQSIESYWSNQSSEESRLYSTQAIALILK
                     SVFSAIEDKDESSMANLSLAAHLSGKAINIAKTTACHAMSYGLSYNFKIPHGIAVAIF
                     LPSVYLYNSKTDSASCGDPRGLGYVKSMFSNLNRLFGCNDAEEVSKKLRGMLNSFGVD
                     GLGSLGVRENDISFLAQQVNIERLNNNPRLVTKMDVYSFYKENL"
     gene            complement(2842..>3641)
                     /locus_tag="P5056_03965"
     CDS             complement(2842..>3641)
                     /locus_tag="P5056_03965"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRY62894.1"
                     /translation="HIKQGKITEKLLDVLSIPHIVLSADMAEEKMSSDIFRLVETSKK
                     ENCPCAIIVKSGLFEPYIKLKKETSFLGSLGREEAVGMVLDSLDGSEIVVSTTGKISR
                     ELYESRKKRDGGSGKDFLTVGSMGHASQIALGISHNKKDKQVYCLDGDGAVIMHMGSL
                     ATTGWVAPHNFKHIILNNMAHESVGGQPTAAEVIDLPGIAKSCKYSFVSSVSTGEELK
                     KTLATFKTATGPALLEIKVGLGSRKDLGRPTESPVENKKKFMDFVGR"
BASE COUNT         1092 a          785 c          533 g         1231 t
ORIGIN      
        1 ccagcttctg tcgtgcgagc cccattaaat ccacactctt ttacaatatc ggcagtttct
       61 ttgttgtaag aaccaaatgg ataacaaaac atttccactt ctttatttat tactttttcc
      121 agccattctt tactcccaca tatttctttc tttttttctt cattccgcaa gatgtgcaaa
      181 tctgggtgtg taagtgtatg cgcacctatt tcatggcgca gagcaatctc gcgaatatta
      241 tcttctgaaa gcggattttc tctgtatttt tgtggaacat aaaatgtccc ctttacgcca
      301 taatggtcaa gaagtcgcga caactttaga tcaagaatat caccgtcatc ccaacttgta
      361 gtaaaaataa cactcattta cttatcttta ttctcattaa tcgcatccca atatttaagt
      421 tttaaccaaa aagtccaaaa ctttcttctt attttttctt tttcaaaaga ataaccgaag
      481 cggttatttc cggcgaccca ccccttgcca ttagtttgcg gtacttgttt tttgttatat
      541 ttttcggtga tatcatttat catcctcatt ttttttcgaa agacttcaga atttaaaact
      601 gaaacctcat caagattggt aaatatatct ttttttagac tattacttaa caatccactc
      661 tcagacgcta atcccaatat gatggccgac ataccgagtg gttgaaaaat aataaaagaa
      721 tcaacttcct ttgacctcca acctaaattt atttttattt ttttacaaga attaatagac
      781 aatctcaaag cctgctccgg agttttaatc gtatcagaat acaaacgcct atctctaaaa
      841 gtattcaaat agacattttt gccgttttta tccaaataag aacgactcag aatattcgta
      901 ttcttaatac cgacatcatt aacctttttt atctgagaat tttttgatat ccattcgctc
      961 aaaataaaag ccgactcaaa accattaagt aagcccttgt aaaatatttt tattaaacgg
     1021 cgtatagctt gctcttgttc caaattaaac caatttgatc ttaattcaga aaattcttca
     1081 gaaaaagtct gatcaatttt tttaccggtg atagaactta cctctcttgc tatatatata
     1141 caactcttaa tttcttcaaa aacagatctt acaggaagct ctttctgggc atacaaagga
     1201 atagcggaat atatcgccga caaatgttcc tcaacattaa gtgcgcttaa aatttcataa
     1261 tcttctttgc tgaattttat tttttcaaaa tttagatctc ttgtgatgcc gggtttctta
     1321 tacaattggc tgtgtccatt cggccaaata ttcattgtcc atgggtcaag ataaagcatc
     1381 ttgcgatagt tttcttcagt aataagaata atttgatgct tcattatatg tcttgtcttt
     1441 ttagacaaaa tcggcaaagg aaaaatatac atcttttctg agtcatccgg aaatactgcc
     1501 caaaaatcca tatctgatat acccggagca ggaatatatc catattcaaa caatgccaaa
     1561 agttcggggg tattttctgc ctctttaatt atctccgccc tggctccatc gtagtcctct
     1621 ttcgtatact taacaggcaa gtcataaacc ctccagccgt ctatctctat tatattttcc
     1681 ttatcactct tgttcataaa ttttctttat aaaatgaata cacgtccatc ttggtaacga
     1741 gtctggggtt attattaagt ctttcaatat taacctgctg tgccagaaaa gaaatatcat
     1801 tttcccttac cccaagactg cccagaccat ccaccccaaa actatttaac atacccctta
     1861 gtttctttga tacctcctct gcgtcattac aaccgaataa tctgtttaga ttggagaaca
     1921 tagatttaac atagcctaaa cctctcggat cgccacatga agctgagtct gtcttactat
     1981 tatataaata aacactcggc aaaaaaatag ccacagcaat accatgcgga attttgaaat
     2041 tataactcaa accataagac attgcgtgac aagcagtagt tttagctata ttgatcgctt
     2101 tcccggataa atgtgccgcc aaactcagat tagccataga agattcatct ttgtcttcta
     2161 ttgccgaaaa gacgctcttt aatattaatg ctatcgcttg cgtggaataa agacgagatt
     2221 cttcgctaga ctgatttgac cagtaagatt ctattgactg agataacgca tccagtgcac
     2281 taggaattaa tttattctta ggaatactta aaattaaatt tgggtcaatt atagaatatt
     2341 tcggtaaaat atattcattt tgcagtgaat atttaactcc atctttatat acaaccgcga
     2401 agcttgtggc ttcactaccg gaacctgctg tagttggaat ggccacaagt gcctttccgg
     2461 agttgtttat tttattgtcg ccaataatgt aagtatctgc gtcctgtggc tgcgctgata
     2521 atatattaat agactttgcc atgtctataa cagaaccacc cccaatcgcc acaataatat
     2581 caggatcgaa tgacctatat atatctatgc cttttttaat atcttcacag agaggtagcg
     2641 aggagaaact attaaattgg agtagctcaa aatcatcttt tatgctctca aaataatctt
     2701 tggctccaga agaaatataa gaatcttttc ctgtgaccaa aaaaatcttt ttagaatttg
     2761 ttttatctaa aatttctcta accttagaga tggtcccctt accgatatat tcaatttgtt
     2821 ttgtaaccat aaaaacataa attatcggcc gacaaaatcc ataaactttt tcttattctc
     2881 aacagggctt tctgttggac gccccaaatc ttttcttgat cctagtccta ccttaatctc
     2941 aagaagcgca ggaccagtag ctgtcttaaa agtagccagc gttttcttta attcctcgcc
     3001 cgtgctaacg cttgatacaa aagaatattt acaacttttg gcgatacccg gtaaatcaat
     3061 tacctctgca gcagtcggct gcccaccgac tgattcgtga gccatattat taagtattat
     3121 atgtttgaaa ttatgtggcg ctacccatcc cgttgttgca agcgatccca tgtgcatgat
     3181 aacagcgcca tctccgtcta aacagtaaac ctgtttgtct ttcttattgt gagatatccc
     3241 caaggcaatc tgtgaggcgt gacccataga gccaactgta agaaaatctt ttcccgaccc
     3301 accatctctc tttttcctgg attcatacaa ttcacgcgaa atctttccgg ttgtactaac
     3361 aactatctcg ctcccatcca ggctatcaag caccataccg acagcttctt ctcttcctag
     3421 cgatcccaaa aaagatgttt ccttttttaa tttaatatat ggttcaaata atccgctctt
     3481 tacgattatg gcacagggac aattttcctt tttggatgtt tctactaatc gaaagatatc
     3541 tgatgacatt ttctcttcag ccatatcagc cgataataca atgtgcggga tagataaaac
     3601 atccaataat ttttctgtaa tcttgccctg tttaatatgt t
//
LOCUS       DAOTEX010000069         3561 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3118
            NODE_19777_length_3561_cov_3.983742, whole genome shotgun sequence.
ACCESSION   DAOTEX010000069 DAOTEX010000000
VERSION     DAOTEX010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868142
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 883
            CDSs (total)                      :: 833
            Genes (coding)                    :: 829
            CDSs (with protein)               :: 829
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3561
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19777_length_3561_cov_3.983742"
                     /isolate="AMR_MDS_3118"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="P5056_03970"
     CDS             <1..242
                     /locus_tag="P5056_03970"
                     /inference="COORDINATES: protein motif:HMM:NF027718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF5011 domain-containing protein"
                     /protein_id="HRY62895.1"
                     /translation="ADTNLGIQKSTFSGDSLISDTDATVDTTAPATFTIKYNATDSAG
                     NKAVEVTRTVIVETISEDAETIIPPAEPIPATPTE"
     gene            282..737
                     /locus_tag="P5056_03975"
     CDS             282..737
                     /locus_tag="P5056_03975"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62896.1"
                     /translation="MVNIICYCLNPNHFHLILEQVSDNGIREFLRKIGGYSRHFNLKY
                     KRSGPLFQGRFKAKRIDTDDYLLHLSVYVNLNDRVHQLRNPSTKLIENSSWKEYIDDR
                     VKGICKKDYVLDNFKSKDEYKKYAENVLQGIIERKNDLAQLENITLEDY"
     gene            complement(775..>3561)
                     /gene="dnaE"
                     /locus_tag="P5056_03980"
     CDS             complement(775..>3561)
                     /gene="dnaE"
                     /locus_tag="P5056_03980"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HRY62897.1"
                     /translation="LGGELSQALWNKDYERARNIALEYQDIFGKENYFVEIMYHPKLE
                     RFLEIRDETVKLARELGIPLVGTQDSHYLRHDDNQAHDTLVAIQTNTDIMDEDRLSNA
                     AEDFSFISTAEANENFKDFPDAIESTTKIADMVDINLTFGNWVFPNIDIPEGRTADEE
                     LRKLTYEGISRRGLEKNDELLARIEYELKVIKDKGYSPYFLVVADLLRFSRENGILTN
                     IRGSVAGSLVTYLNFITNVNPIEFSLPFERFLNPERPSAPDIDMDYADNRRDEVIEYA
                     KRKYGADKVAQIGTFGTMMARGSVRDVARALAYPYEVGDRISKMIPMGAQGFPMTIKR
                     AMEEVPELKTAYETEKDTKIIMDLAMKIEGTVRHCSVHAAGVVIAPTTLTDWVPLQIE
                     PRAQKVITQYDMHVVEDMGLLKFDFLGIRNLAILGDAVRLVKKYHNVDVDLDNMPMDD
                     KKTFQMLARGETMGLFQLNGEGMTKYLKELKPSTMHDINAMVALYRPGPIESIPLYIE
                     RKNNPKLVQYLDPRLEPLLKKSFGVITYQDDVMLIAIKLAGYSWLEADKLRKAMGKKI
                     PEEMQAQKIKLLEGFKKGGMTEAKAQELWALIEPFASYGFNKAHAASYGRVAYQTAYL
                     KANFPAEYMNAVLTAESDDIEKMGVIMSECKRMGIPVLQPDINQSELGFAIIKGKDGN
                     GDRIRFGLNAIKNLGTEIAQFIIDERVKAGEFQTFGDFLSRVKHRNLNRKSLEALIKG
                     GAMDTFSERGMLLQNMDNALSFNKELARDNGAQASLFGLLPKNTAAPEFKLQNFPPAT
                     LKEKLDWEKELLGLYLSGHPLQNFEEKFKKKENTIAYIKTQGNGKPVVAGGIINEVKQ
                     ITTKKSEQMAFVKISDLNDSIEIVVFPKTLTQYKNLLVPDKVVAVKGTISLRNDVPGI
                     IADAVKELVPDDKK"
BASE COUNT          941 a          862 c          729 g         1029 t
ORIGIN      
        1 cggccgacac aaatctcgga atacaaaaat ctactttctc gggcgacagt ctaatttcag
       61 atactgatgc cacggtagat actaccgccc ccgcgacatt tacaataaaa tataacgcca
      121 cagacagcgc cggcaacaaa gccgttgagg taacccgaac ggtgatagta gagacgatta
      181 gtgaggacgc cgagactatc atcccacccg ccgagccgat acccgctacc ccgaccgaat
      241 aaataaacta atcttaacaa cttaggaacc cgtctactaa gttggttaat attatttgct
      301 attgtctaaa tccaaatcat tttcacctca ttctagaaca agtttcagac aacgggatac
      361 gggaattttt aagaaaaatt ggaggatact ccagacattt caatttaaaa tacaaaagga
      421 gcggcccctt gtttcagggt agatttaagg ccaagcgtat agataccgac gactacttat
      481 tacatttgag tgtatatgta aacctcaacg atagagttca ccaacttagg aacccgtcta
      541 ctaagttaat tgaaaattca agctggaaag aatatataga cgacagggta aaaggtatct
      601 gcaagaaaga ttatgtatta gataatttca agagcaagga cgaatataaa aaatatgcgg
      661 aaaatgtgct tcaaggcatt attgaaagaa aaaacgactt agcccaatta gaaaacatca
      721 ccctagagga ttattaagtt ttatgatgac ttagtagacg ggttcctaag ttgattattt
      781 tttatcgtcg gggactagct cctttaccgc atctgcgata atgcccggca cgtcgttcct
      841 taaagaaatg gtgcctttca ctgcgaccac cttatccggg acgaggagat ttttgtattg
      901 cgttaatgtt ttagggaaca cgacgatctc tatggagtca ttcaagtcgc ttatcttcac
      961 gaacgccatt tgttccgact tcttcgttgt tatctgcttt acctcattta tgattccgcc
     1021 cgccacgaca ggcttgccgt taccttgggt ttttatgtaa gcgattgtat tttctttttt
     1081 cttaaatttt tcttcaaagt tctgaagagg gtgtccggac aaatagaggc cgagcaattc
     1141 tttttcccaa tccagttttt ctttgagcgt agcgggagga aaattctgta atttaaattc
     1201 aggtgcggcg gtatttttcg gcaagagccc gaagagggag gcctgcgcgc cattgtcccg
     1261 cgccagctcc ttattgaaag acagggcgtt gtccatattt tgaagtagca ttcctcgctc
     1321 actaaaagta tccatggcgc cgccttttat aagagcttca agcgactttc tgttcaaatt
     1381 tctgtgtttg accctcgaaa ggaaatctcc gaacgtctga aactctccgg ccttaaccct
     1441 ctcatcaata ataaattgcg cgatctctgt gccgagattt ttaatcgcat ttaatccaaa
     1501 cctgatccgg tctccgtttc cgtccttgcc cttaataatc gcgaatccga gctcgctttg
     1561 atttatatcc ggctgaagca ccgggattcc catcctttta cattcggaca taatcactcc
     1621 catcttctcg atatcgtcag actccgccgt caaaacagca ttcatatatt cggcggggaa
     1681 gttcgccttc aggtaagcgg tctggtaagc gacacgaccg tagctcgcgg cgtgtgcctt
     1741 gttgaatccg taagaagcga atggctcgat gagcgcccaa agttcctgag ccttagcctc
     1801 cgtcattcct ccttttttga aaccttccag aagctttatt ttttgggcct gcatttcctc
     1861 cgggattttc tttcccatcg cttttcgaag cttgtccgct tctagccagg aataccccgc
     1921 gagcttgata gcgatgagca taacgtcgtc ttggtatgtg ataacaccga aagacttctt
     1981 caaaaggggc tccagcctag gatccaggta ttggaccaat ttagggttat ttttgcgttc
     2041 tatatataga ggaatagact cgataggccc cggacgatat aaggccacca tagcgtttat
     2101 atcatgcata gtgctcggtt taagctcttt cagatacttt gtcattcctt caccattcag
     2161 ctgaaatagg cccattgttt cacccctggc gagcatctgg aaggtctttt tgtcatccat
     2221 cggcatattg tccaaatcga catcaacatt gtgatatttc ttcacaaggc ggacggcgtc
     2281 tcccaaaatg gccaaatttc taatccccaa aaagtcgaac ttgagaagtc ccatatcttc
     2341 cacaacgtgc atatcatatt gagtgatgac cttctgtgcg cgaggttcta tctgcagggg
     2401 aacccaatca gtgagagtag taggagcgat aacaactccc gcggcgtgaa cactgcagtg
     2461 cctgaccgtg ccctcaatct tcattgcaag gtccattatg atctttgtat ctttttctgt
     2521 ttcgtaagcg gtttttaatt ctggaacttc ttccattgct cgcttaatgg tcatagggaa
     2581 accctgcgca cccataggga tcatttttga tattctgtct ccaacttcat aagggtaggc
     2641 gagcgctctc gcaacgtctc tcacagaacc tcgcgccatc atcgttccaa atgtgcctat
     2701 ctgtgcgact ttgtcggcgc cgtatttcct cttcgcatat tcaataacct catcacgccg
     2761 attgtccgca taatccatat cgatatcggg agcggaaggt ctttccggat tcaaaaatct
     2821 ttcaaaaggc aagctgaact ctatcggatt aacgttcgta atgaagttga gataagtaac
     2881 aagagagcct gcgaccgaac ctctgatgtt tgtgaggatg ccattctcac gggaaaaacg
     2941 caaaaggtcc gccaccacaa gaaaatacgg agaatagcct ttatctttaa taaccttgag
     3001 ctcgtattct attctagcca aaagctcatc atttttctct aaacctcttc gcgagatacc
     3061 ctcatatgta agtttgcgaa gctcttcgtc ggctgttcta ccctccggaa tatctatgtt
     3121 cggaaatacc caattaccaa aagtgagatt tatatctacc atatcggcaa tctttgtcgt
     3181 gctttcaatc gcgtcgggga agtctttgaa attttcgttc gcttccgcgg tgcttataaa
     3241 agaaaaatct tcagccgcgt ttgaaagacg gtcctcgtcc atgatgtcgg tattcgtctg
     3301 aatagccaca agcgtgtcgt gcgcctgatt atcgtcgtgc ctcaaatagt gcgaatcttg
     3361 tgtaccgaca agaggaatcc caagctccct ggcgagcttc acagtctcgt ctcttatctc
     3421 caaaaatcgc tccagcttcg gatgatacat tatctctaca aaataatttt ctttcccgaa
     3481 tatgtcctga tactcaagtg caatatttct ggcgcgctca tagtccttgt tccaaagcgc
     3541 ctgagataat tctccgccga g
//
LOCUS       DAOTEX010000070         3415 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3118
            NODE_21085_length_3415_cov_3.543452, whole genome shotgun sequence.
ACCESSION   DAOTEX010000070 DAOTEX010000000
VERSION     DAOTEX010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868142
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3415)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3415)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 883
            CDSs (total)                      :: 833
            Genes (coding)                    :: 829
            CDSs (with protein)               :: 829
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3415
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21085_length_3415_cov_3.543452"
                     /isolate="AMR_MDS_3118"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1731
                     /locus_tag="P5056_03985"
     CDS             <1..1731
                     /locus_tag="P5056_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62898.1"
                     /translation="KIEIVDLGNGGNWTVGQPVTIKWKETGLTSPIKAYLRKPNTRAT
                     DGVCYLGQANVNTGLLNFTVSGGADCISISGFGFTNKVISAGKYTLVLESGNTKVSSA
                     LSGVNVSSFPSITLNDIGNEGNWFFDQPISIKWSYTGLTNPSFSVFLQKSNAAPGDMC
                     RLNSVEPFKSSQLDYTPKNAYGCFGNNGSTSAFPNKSLPAGEYKLIVRTVFGGKNIEA
                     ISKTITVKKAPKIFVKQPVVGEYAAGGYLTASWMTEGFTENLKIELANSAGDAVVASL
                     LGNVLQTPSSISALIPASVTAGDYRVRISSVSNPKIFGLSNIFKIKAANTSCVTDGGS
                     MGELGLRIKLCCAGLVQVPDDYTKAPEQRNEKTYGTCMKNYPADMWTSSSAGLQTYRW
                     GDTLSFKWNIEKAGGSYNSVTVEIWEAPNGTSGALGNRVYIRSQINNGLFTMPAPSSF
                     DRKAFLGKYVIRVKPNVVRNIVFQSPVFNVVSGEEIVVTTPGTTSTTPGFPIKVLWKS
                     YPQKGNVYVNLIDSSGKNITNVMVRNNGYYVLTIPKTIKEGAYRVRVTSAEKRSLFGQ
                     SLFFFVSARG"
     gene            1876..>3415
                     /locus_tag="P5056_03990"
     CDS             1876..>3415
                     /locus_tag="P5056_03990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62899.1"
                     /translation="MLNFKKYFVALVVIFPAFFWFVPVASAASITVVSPTGSSVFNGT
                     TDQIRFSFYSEGIPSLSGSNDVRMAEEVLFEDGRVALTATQMLGSSYFNHFSIGLTGQ
                     QTLSISSSRLSQGRYRWRIYSYDYPSIEAKSVVFTVGSPAPAPTVVTPAPVAPTINSF
                     TADSLGVNPNDTTTLRWSVSNATECSINGVSVSRISGSRITDNLTSATTFTLNCLNGT
                     VPASQSVTVRITPATLAITDKGNNNTGVWTIGQPITIKWRGTGLSMLSQVKLKKNNLI
                     LADGYCTLERDAFGTDATVHLWGGASGIPDCNPFSGSGFTEGVVAPGEYTLIIENGEV
                     SATAPITVNPAPVAPTINSFTADSLSVLRGESTTLRWNVTGANRCTLMNMNYIDAVEE
                     LAVSPITTGAINFLTRYKLTCYNGSLSSYKTLSISLAQPVIDSFTADNESILAGQSTN
                     LRWNVRGALDCYITGGAMSLYSVNAVSGSKPTGALNNSTDFELWCRNGTGLPSGERKL
                     STRID"
BASE COUNT          954 a          666 c          831 g          964 t
ORIGIN      
        1 aaaattgaaa ttgtggattt ggggaatggc ggaaactgga cggtaggtca gcctgtgaca
       61 atcaaatgga aggaaacagg tttaacttct cccataaagg cttatctaag gaaaccaaac
      121 acgagggcca cagatggtgt gtgctatttg ggacaagcta acgtaaatac cggccttctc
      181 aattttactg tcagtggagg agcggattgt attagtatct ccgggttcgg atttacaaat
      241 aaggttatat ctgccggtaa atatacgctg gttcttgaga gcggaaacac aaaagtatca
      301 agcgcgctta gtggtgtaaa cgtttcatct tttccatcaa taaccctgaa cgatatcgga
      361 aatgaaggaa attggttttt tgatcagcct attagtatca aatggtcata caccggcctt
      421 accaatccgt catttagcgt atttttgcag aagagtaatg ccgctccagg ggatatgtgt
      481 cggctcaatt ctgtggagcc cttcaaatcg tcacagctag actatactcc gaagaacgct
      541 tatgggtgct ttggaaataa cggatcgaca agcgcttttc caaataaatc gttgcctgcg
      601 ggagaatata aattgatagt caggaccgtt tttggcggta aaaatatcga ggcaattagc
      661 aagacgataa cggtcaagaa agccccgaag atatttgtaa aacagccagt cgtgggcgag
      721 tatgccgcgg gaggatattt gaccgcttcg tggatgacag aaggatttac ggaaaatttg
      781 aagatagagt tggcgaatag tgccggtgat gcagtcgttg catcactttt gggtaatgtt
      841 ttgcaaacgc catctagtat cagtgccctt attccggcaa gtgttacagc cggagattat
      901 cgagtcagaa tatcttccgt ttctaatcca aaaatcttcg ggctaagtaa tattttcaaa
      961 ataaaggccg ctaatacttc ttgcgtgaca gatgggggca gtatgggaga actcggcctc
     1021 agaataaagc tttgttgcgc cggcttggtg caggttcctg atgattacac caaggctccg
     1081 gagcagagaa acgaaaaaac ttatggaacg tgtatgaaga attatccggc cgatatgtgg
     1141 acttcatctt ccgctgggct acagacatac aggtggggag atactcttag ctttaagtgg
     1201 aatattgaga aagccggcgg ctcttacaat agcgtcacgg tagaaatatg ggaagcaccg
     1261 aatggcacat ccggagctct cggtaataga gtttatatac ggtcgcaaat aaacaatgga
     1321 ttgtttacga tgccggctcc ttcttctttt gatagaaagg cgtttttggg aaaatacgtt
     1381 attagggtta agccgaatgt tgtcagaaat atagtattcc agagccctgt atttaatgtt
     1441 gtaagcggag aggagatagt ggttaccacg cccggcacaa cttccactac cccgggattc
     1501 cctatcaaag tattgtggaa gtcataccct caaaagggta acgtatacgt gaatttgatt
     1561 gattctagcg ggaagaatat taccaacgta atggtccgga ataacggtta ttatgtcctt
     1621 acaatcccga aaacgataaa ggaaggcgcc tacagagtaa gggtcacttc ggccgaaaaa
     1681 agaagtcttt ttggtcagag cttattcttc ttcgtgtcgg caaggggata aattatattt
     1741 cttaaaaaca ccccgataat tacggggtgt ttttggtttt ggcggtgcgt ttctttcgtg
     1801 cggatgggcc ggggagatgt tataattaag gagtaaaaat ttattaaaaa taaatcgcta
     1861 attttattat aaaaaatgtt aaattttaaa aaatactttg tcgctctggt tgtaattttc
     1921 cccgcatttt tctggtttgt tcctgtggcg tctgcggctt ctattacagt tgtttctccg
     1981 acaggctcaa gtgtttttaa tggaactact gaccagattc gtttttcctt ttattctgaa
     2041 ggaattccaa gtttatccgg ttcaaatgac gtaaggatgg cggaggaagt gttgtttgaa
     2101 gatggacgtg tcgccttaac cgcgacacaa atgttggggt cgtcttattt taatcatttc
     2161 tcaatcggtc taacaggaca gcagacattg tctatttcct ccagcagact gtcacaagga
     2221 agatataggt ggagaattta ttcttatgat tacccaagca tagaggccaa gagcgttgta
     2281 tttactgttg ggagtccggc accagcgcca actgttgtca cccctgcgcc agtcgcgcct
     2341 actataaata gttttacggc tgactcttta ggcgtaaacc ctaatgacac aacaacctta
     2401 agatggtctg tgtctaacgc aaccgaatgt tctatcaatg gcgtttctgt cagtcgcata
     2461 tcaggatctc gaattaccga taatttaaca tcagctacaa catttacgct caattgttta
     2521 aatggaacag tgccggctag ccagtcggtg actgtgcgca tcactcctgc aacgctggca
     2581 ataaccgaca agggaaacaa taacactggt gtttggacaa ttgggcaacc tattacaata
     2641 aaatggcggg ggacgggttt gtctatgctt tctcaggtca aattaaagaa aaacaacctt
     2701 attctggctg atggttactg tactttagag agagacgctt ttggcacgga tgcgactgtc
     2761 catctgtggg gtggtgcgtc gggtattcct gattgcaatc ctttttccgg ctccggtttt
     2821 actgaggggg ttgttgctcc gggagaatac actttgatta ttgagaatgg cgaggtgtcc
     2881 gctaccgctc cgataactgt aaaccccgcg ccagtcgcgc ccactataaa tagttttact
     2941 gccgatagtt tgtcggtttt aaggggggag tcaaccacgc ttcgctggaa tgtgacaggt
     3001 gcgaacagat gcaccttgat gaatatgaat tatattgatg cggttgagga gctcgcagtc
     3061 agccccatta ccacaggtgc cataaatttt ttaacaagat ataaattaac ttgttacaat
     3121 ggtagcttat ctagttacaa aactctttct ataagcttag ctcagccggt catagatagt
     3181 tttaccgcgg acaatgaatc aattttagcc ggacagtcca ccaatcttag gtggaatgtt
     3241 aggggagcat tagactgtta tatcacgggg ggagccatga gtctttactc agttaatgcg
     3301 gtttcgggga gtaaacctac gggcgcctta aataattcta ccgattttga attatggtgc
     3361 agaaacggaa ccggtttacc aagcggagaa agaaaactgt ctacgaggat agacg
//
LOCUS       DAOTEX010000071         3393 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3118
            NODE_21286_length_3393_cov_3.613841, whole genome shotgun sequence.
ACCESSION   DAOTEX010000071 DAOTEX010000000
VERSION     DAOTEX010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868142
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 883
            CDSs (total)                      :: 833
            Genes (coding)                    :: 829
            CDSs (with protein)               :: 829
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3393
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21286_length_3393_cov_3.613841"
                     /isolate="AMR_MDS_3118"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..271
                     /locus_tag="P5056_03995"
     CDS             <1..271
                     /locus_tag="P5056_03995"
                     /inference="COORDINATES: protein motif:HMM:NF021567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2293 domain-containing protein"
                     /protein_id="HRY62900.1"
                     /translation="VRLFPGCPPELKVKIVLDAVYRHNSCVHNMTLSWDRKAELVFEA
                     WSRHNCTSYDSCLDGGTSRSKARRDVVGMVSFVLSEWRKSKPKQA"
     gene            622..2547
                     /locus_tag="P5056_04000"
     CDS             622..2547
                     /locus_tag="P5056_04000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62901.1"
                     /translation="MEDNFDEGGIKKTEKRLYSQTPGNLERRRSGLSDVNYDEKVTWG
                     GEEGLNLRNMVQTRKKFPILSFVLTLSFAFFVGAIIFAAYLYFGNTNIISPSNVDISI
                     VGPVSVKAGEEINLQVAITNKNNTALEYVELITTFPGGSKILTNKVNDTQSRKNLGTI
                     QPNQIITETIKTALYGEENADREIKVGIEYRNQNSNAIMAKDALWKTVISSSPLSLIF
                     DLPADANSNQEITLNVKTISNSKDPLTDVVLNVAYPAGFIFKESVPKASSGTGVWFLG
                     DMTQGDEKLVKIKGVIQGQNDEERSFRAIAGQRTSRDTTKVDVAYASSEKSLIIKKPF
                     IGATLAFNGNQEDTYIITDGQTVRGDIEWENNLPVDLLHAEVIVKILGEVLDKSSVSP
                     SLGFYNSLDNTIIWNKSSGGLPDLISSGQGNRLSFSFNPLPLLSRNRTIFKNPELNFE
                     LSFKGYKSSETRAEDAVVETTLKKKIRISSNLQVSPRALYYSGPFKNTGLLPPVHDEE
                     TTYTVVWSVVNSSNDVSDVVVKTTLPPYVRWLGVVSPTTEGITYNPLGGEIVWNVGAV
                     KAGGGITVPAREVAFQLGMTPSISQVGQNPNITSDTTITGHDDFTGKEVTHSRKAVSS
                     QLTSDPLVQGESSGFVK"
     gene            2692..>3393
                     /gene="glyS"
                     /locus_tag="P5056_04005"
     CDS             2692..>3393
                     /gene="glyS"
                     /locus_tag="P5056_04005"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013777758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HRY62902.1"
                     /translation="MIMNTKNEENLLDKIVSLAKRRGFIFRGSEIYGGLGGTWDYGPL
                     GVALKNNIKNLWWKKFVDDREDMYGVDAAVLMNPKVWESSGHVATFTDPLVECAKCKR
                     RFRADHVDGDKCPECGGKIGEARQFNMMFKTNVGPVEDSSSVTYLRPETAGGIFMNFK
                     NVLDSFHPKLPFGIAQIGKAFRNEITPKDFIFRVREFEQMEIEYFIRPEGWENIFEDW
                     RKAILEYAEEIGVNMK"
BASE COUNT          967 a          675 c          851 g          900 t
ORIGIN      
        1 tgtgcgcctg tttccggggt gccccccgga gttgaaagtg aagattgtac tggacgccgt
       61 ttatcgtcac aattcgtgtg tgcataatat gactttgagt tgggatagaa aggccgagct
      121 cgtgttcgag gcatggtcac gtcacaattg taccagttac gatagttgtc ttgatggagg
      181 aacatctcgc tccaaagcgc ggcgtgatgt tgtcgggatg gtctcattcg ttcttagcga
      241 atggagaaaa tctaaaccta aacaggctta atcctaaatt gggcgcttgc ccacgaattc
      301 tgggaaaccg gagggaggaa caggtatgat ccactaccga cgactcctct tttcgcgatt
      361 tacgagaaag aaaggagcgg tgttctacac cgcaccctct attacgagat tatcccgccg
      421 aagcagatcg aggccgtcgg gtaggaccga agtctcacac gccgcctgtg cacaatgcat
      481 gggcggcttt tacttgtccg cttcacccgc ctaaatttat ttcataaatt ttggtgggca
      541 agaaattttc tcggaaaatt tcagaggatg ttgtataatt atgctaggca tttaaccgta
      601 tttttttata ttacaaaaca aatggaagat aattttgatg aaggaggaat aaagaagaca
      661 gagaagcgtc tttattctca aactcccggt aacctagaga gaagaagaag cgggctttcc
      721 gatgttaatt atgacgagaa ggttacttgg ggaggggaag aagggttaaa tctaagaaac
      781 atggtgcaaa ctagaaaaaa attccctata ttgtctttcg tcctcactct ttcttttgcc
      841 ttttttgtag gggcgataat ttttgccgct tatctttatt tcggcaacac aaacattatc
      901 tctccgtcca acgttgatat ttcgatagtc ggtccggtct ctgtaaaagc gggagaagag
      961 ataaatttgc aggttgctat aacgaacaag aataataccg cgcttgaata tgtggagctt
     1021 atcacgacat tcccgggcgg ttcgaagata ctcacaaaca aggtgaacga tacccaatct
     1081 aggaaaaatc tcggcacaat acagccaaat caaataatta ccgagaccat aaagaccgct
     1141 ttgtatggtg aggagaacgc cgatagggag ataaaagtcg gaattgaata cagaaatcaa
     1201 aattcaaatg cgattatggc gaaggacgct ctttggaaga cggttatcag ttcgtctccg
     1261 ctttctctca tattcgactt gcctgccgat gcgaattcaa atcaggagat taccttaaat
     1321 gtgaagacaa tctccaattc aaaagatccg ctgactgatg ttgttttgaa cgttgcctat
     1381 ccggccggtt ttatttttaa agaatctgtt ccaaaggcct cctccggaac gggagtatgg
     1441 tttttgggtg atatgacgca gggcgatgaa aaattggtaa agataaaagg tgttatacag
     1501 ggccaaaatg acgaagagag gtccttccgt gccattgccg gtcagcgcac gagcagggac
     1561 acaacaaaag tggacgtggc ttacgcttct tcggagaaga gcttgattat aaagaagccg
     1621 tttattgggg ccacacttgc gtttaacggt aatcaggagg atacgtatat tattacagac
     1681 gggcagaccg taaggggaga tatagaatgg gagaataatc tgccggtaga cctactccac
     1741 gccgaagtga tcgtcaagat attgggtgag gttctagaca agtcgtccgt gagcccttcg
     1801 ttaggttttt ataattccct ggataacaca atcatctgga acaagtcgag cggaggtttg
     1861 cctgatctta taagttccgg tcagggcaac agactttcct tttcgttcaa tccgcttcct
     1921 ctcttgtccc gcaataggac cattttcaag aaccctgaat tgaattttga gctctcgttc
     1981 aagggatata aatcatccga gaccagagct gaagatgcag tcgtcgagac caccctaaag
     2041 aagaagataa gaatctcttc aaacttgcaa gtttctccta gggctctcta ttattccgga
     2101 cctttcaaga acacagggct attgccgccg gtgcacgatg aggagaccac atacacagtt
     2161 gtatggtcgg tcgtaaattc ttcgaatgat gtttctgatg tggttgtgaa gacgaccttg
     2221 ccgccttatg taagatggct cggcgttgtt tctcctacaa cagagggtat tacatacaat
     2281 ccgttgggcg gcgagatagt ctggaacgtt ggcgccgtaa aggcgggagg cggtataact
     2341 gttcccgcga gagaggtggc attccagctc ggaatgacgc caagcataag ccaggttggc
     2401 caaaatccaa acattacttc agacactaca ataaccgggc atgatgactt taccggcaag
     2461 gaagttacac acagcagaaa ggccgtatct tcccagctta cgagcgatcc tcttgttcag
     2521 ggcgaatcat ccggatttgt taaatagttt ctcaatagag cgttctcctt gttgtcattc
     2581 ccgcgaaggc gggaatctaa cgagacagat aaagagatgg attcccgcct tcgcgggaat
     2641 gacaaagacg tgagatagga ttgaagtttt gttaattttt agtagtattt gatgattatg
     2701 aatacaaaaa atgaagaaaa tctgttggac aagatagtct cgctcgcaaa gaggcgcggg
     2761 tttattttta gaggctctga aatatatgga ggcttgggcg gtacttggga ctacgggccg
     2821 cttggtgtgg ctttaaaaaa caatatcaag aatctttggt ggaagaaatt tgttgatgat
     2881 agggaggata tgtatggagt cgacgccgcc gttcttatga atccaaaagt ctgggaatct
     2941 tcgggacacg tcgccacttt tactgatcct ttagtagagt gcgcaaaatg caagagaaga
     3001 tttagggcgg accacgtaga cggggacaaa tgccctgagt gcggaggcaa gataggagag
     3061 gccagacaat tcaatatgat gttcaaaacg aacgtcggcc cagtagaaga ttcttcctct
     3121 gttacatatt taagacctga aactgccggc ggtattttta tgaacttcaa gaatgttttg
     3181 gattctttcc acccaaagct tccttttggt atagcccaga tcggtaaggc gtttcgaaac
     3241 gaaataactc caaaggattt tattttccgt gtgagggaat ttgagcagat ggagatagag
     3301 tattttatta gacccgaagg atgggaaaat atttttgaag attggagaaa ggcaattctc
     3361 gagtatgccg aggagattgg tgtaaacatg aag
//
LOCUS       DAOTEX010000072         3300 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3118
            NODE_22187_length_3300_cov_3.359938, whole genome shotgun sequence.
ACCESSION   DAOTEX010000072 DAOTEX010000000
VERSION     DAOTEX010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868142
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3300)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3300)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 883
            CDSs (total)                      :: 833
            Genes (coding)                    :: 829
            CDSs (with protein)               :: 829
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3300
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22187_length_3300_cov_3.359938"
                     /isolate="AMR_MDS_3118"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            255..569
                     /locus_tag="P5056_04010"
     CDS             255..569
                     /locus_tag="P5056_04010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62903.1"
                     /translation="MIEIKTRTTLKDMTSDLSTLLFLESELKKRKSLSSKTDLISNHN
                     KFSDIKEVINFLPEEYIAPLINGVELEIKEELREARRKHKVEMHRMENKSLDELIKIN
                     SH"
     gene            645..971
                     /locus_tag="P5056_04015"
     CDS             645..971
                     /locus_tag="P5056_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62904.1"
                     /translation="MGKIYGDVVNTTIKVLKEMGDATVKAAAEQMIRLQSYMIESQSA
                     PKAAGWTEEDGDHVIKNLKALKQMLDTNVDVMIKVTQDASGMQVDGDQLAIDGYKLSD
                     VSPDTN"
     gene            1030..1194
                     /locus_tag="P5056_04020"
     CDS             1030..1194
                     /locus_tag="P5056_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62905.1"
                     /translation="MKLKILKNFRVGSVSDPKRMIKGNVIESDSITEFTPERIEKWIK
                     MGWVEKVTFP"
     gene            complement(1240..1407)
                     /locus_tag="P5056_04025"
     CDS             complement(1240..1407)
                     /locus_tag="P5056_04025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62906.1"
                     /translation="MEISEAELEAIKQRSEAIGAINQSLDNIGESVKHINQVLDDAFE
                     KFDKIKERLNR"
     gene            complement(1497..1736)
                     /locus_tag="P5056_04030"
     CDS             complement(1497..1736)
                     /locus_tag="P5056_04030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62907.1"
                     /translation="MTCFIQTWPAKRLPEGVESRILVYSGEDVVFVPDDTEQIFKING
                     NIKKECRIEEKVSGFNYELAPGNLFLGEKLEVVLE"
     gene            complement(1738..2244)
                     /locus_tag="P5056_04035"
     CDS             complement(1738..2244)
                     /locus_tag="P5056_04035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62908.1"
                     /translation="MQTPRLKVTVTSLPGEPSFTMPINATEAEIIREIKDRFPKRKGV
                     EYEWFSQPITIKDRDDIVLVLRERITALEEELTGEKKKSKTLWSGLRKLKRLLIINTA
                     KNCFMERSHPEIEIAPLNQDTREAIKRIDGELIRLSLAELEKSLEVKDELKRIWEEYE
                     MAELMKGE"
     gene            complement(2415..>3300)
                     /locus_tag="P5056_04040"
     CDS             complement(2415..>3300)
                     /locus_tag="P5056_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62909.1"
                     /translation="IGLLSHNAVAVLDNVSMIDRETSDVLCNVVTGGVIPTRELYTTN
                     DQVLIDIRSKLIVTAINSQIFESGSGDLAERTVSIEISRPEDNYVSEEYLNEKFSEYR
                     SVIFGAVLSLVKGYLRDGIPKAGDKSEFRLTTYASVGRYLTKALNLQSPFDKAYRLNQ
                     AGMSQTSLEAEPVTEFFIDFVRQYNGVDFQNSSGWGISTEALFNTYFNKWVAATNDIG
                     LYDQKLPNNSAKLLSRLKRIEPDLRKIYGIRIETTGMHRGKKWIKIYATDMSLLNNEE
                     EEEESEKKAQTSIEEGWG"
BASE COUNT          895 a          727 c          596 g         1082 t
ORIGIN      
        1 atactgagct tgtggccgct tggcttcagc attgcctgca agaggcgatc cgggcgcgag
       61 gatggcgata tgggatagat tatgatacag atcgggtatg tgcaaccgtc ctgaaaaata
      121 tcgattctgc atggaaattg attggagtag gatatggctc caccgaagcc gaggccctca
      181 tgagggcgta cttatccgcg ctctcgggtg agcgtattta gttgcgctct catgcgaggc
      241 atacctggcg gcatatgatc gagattaaga ctcggacaac cctcaaggac atgacttccg
      301 acctctctac tcttcttttt cttgaatcag aacttaaaaa gagaaaatct ctttcttcta
      361 agacagacct tattagtaac cataataaat tttcagacat aaaagaagtt ataaatttcc
      421 ttccagaaga atatatcgcc ccgctaataa atggggtaga actcgaaata aaagaagaac
      481 ttcgagaggc gcgaagaaaa cataaagtcg aaatgcatcg aatggaaaac aaaagtctag
      541 acgagttaat taaaatcaat tcgcattaat aaaatctttt aaatacttcg aaattctttc
      601 ctgttttgac tcctttaatt aaaggacttt ttcaggtgaa ttaaatggga aagatatacg
      661 gcgatgttgt gaacacaact atcaaggtgc tcaaggaaat gggcgacgcc accgttaagg
      721 ccgcagccga gcagatgatt agacttcaat cctacatgat tgagtcccag tccgctccca
      781 aggccgcagg gtggaccgag gaagacggcg atcatgttat taagaacctt aaggctctta
      841 agcagatgct tgatactaac gtagatgtca tgatcaaggt cactcaggat gcttctggta
      901 tgcaggtcga tggcgaccag ctcgctattg atggctataa gctcagtgat gtaagccccg
      961 atacgaatta aagtcgtatc gtccagcaca gtctttcttg actgtgcata tctattttta
     1021 ggtgttatta tgaagctaaa aatattaaaa aattttagag ttggatcggt ttccgacccg
     1081 aaaaggatga ttaagggcaa cgtcatagaa tcagattcta taactgaatt taccccggaa
     1141 aggatagaaa aatggataaa aatggggtgg gttgagaagg ttacgttccc ataacttctt
     1201 cttcttttat tacttgtttt aactttttct attatcgatt taacgattta atctctcttt
     1261 gattttatcg aatttctcaa aagcatcatc aagcacctga ttaatatgct tgacagactc
     1321 gcctatatta tccaagcttt gatttatcgc ccctatcgcc tctgatcgtt gcttaatcgc
     1381 ctctaattcc gcttcagata tctccattca tctcacccgg atcacaatct tagttataag
     1441 ttaaatattc actttagtat cttttgaaca attattttat ttcgtgtcct aaaatttcat
     1501 tccaaaacaa cctccaattt ctctcccaaa aacaaatttc ccggagctaa ttcgtagttg
     1561 aatcctgaaa ctttttcttc gattctacat tcctttttta tatttccgtt aattttaaaa
     1621 atttgctctg tatcatcagg gacaaaaacc acatcctctc ccgagtaaac taaaatccga
     1681 ctttcgactc cttctggtag cctttttgca ggccatgttt ggataaaaca ggtcatttta
     1741 ctcacccttc attaactctg ccatctcata ttcttcccag atccttttta attcgtcttt
     1801 tacttccaat gacttttcaa gttcagctaa acttaagcgg attagctctc catctattct
     1861 tttaatagct tctctggtat cctgattcag aggagctatc tctatttcgg gatgactcct
     1921 ctccataaaa caattctttg cggtattgat tattagaagt cttttgagtt ttcttagtcc
     1981 gctccagagg gtcttactct tctttttttc tccagtcaat tcttcttcaa gcgccgttat
     2041 cctctctctt aataccagaa ctatatcgtc ccggtctttg attgttatcg gctgagagaa
     2101 ccactcgtat tcaacccctt ttctttttgg gaacctatct tttatctctc tgattatttc
     2161 cgcctctgtt gcgttaatag gcatggtgaa agaaggttct ccgggcaatg aagttacggt
     2221 tacctttagc cttggtgtct gcatatctcc ctcctattgt tctgttttaa tctatttctc
     2281 ttttagattc tacaaactct acctgagtaa aatctccaag tccgtaccaa tatctagcaa
     2341 caaaagttgt gggttttatt gacccatgtg aaacatctga tactaaatct agctgaaacg
     2401 gtataaacat ttttttatcc ccacccctct tctatagagg tctgtgcttt tttctctgat
     2461 tcttcctctt cttcttcgtt attaagcagg ctcatatctg tggcatatat cttaatccat
     2521 ttctttcctc tatgcattcc ggtggtctct atcctaattc cgtagatctt tctcaggtcg
     2581 ggttcaattc tcttaagcct ggacaaaagc ttcgcgctgt tattaggaag tttttggtca
     2641 tatagtccaa tatcatttgt tgccgctacc catttattga agtaggtgtt aaacagcgct
     2701 tcagtagata ttccccatcc cgagctgttc tggaaatcta ctccgttata ttgccttacg
     2761 aagtcgataa agaactctgt aacaggttct gcctcaagag aagtctggct cattccagcc
     2821 tgattcagtc tataggcttt atcaaaaggc gattgaagat tcagggcctt tgtcaaatat
     2881 cttccaacag aagcatacgt tgttagccta aattccgatt tgtctcccgc ttttggaatt
     2941 ccatccctta aataaccctt aacgagggat aaaactgcac caaatataac cgagcgatac
     3001 tcgctgaatt tttcgtttaa atactcttct gaaacgtagt tgtcttctgg ccgggagatc
     3061 tctatactaa ccgttctttc tgccaagtct ccagagccgg attcaaatat ttgagagtta
     3121 atagccgtta ctataagttt tgatcgtatg tcgatcaaaa cttgatcatt ggtagtgtaa
     3181 agttcccttg tgggaattac tccacccgtg actacgttgc agagcacatc agaagtttcc
     3241 ctgtctatca ttgagacatt atccaacaca gctactgcgt tatgcgagag aagacctatt
//
LOCUS       DAOTEX010000073         3271 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3118
            NODE_22476_length_3271_cov_3.906405, whole genome shotgun sequence.
ACCESSION   DAOTEX010000073 DAOTEX010000000
VERSION     DAOTEX010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868142
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3271)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3271)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 883
            CDSs (total)                      :: 833
            Genes (coding)                    :: 829
            CDSs (with protein)               :: 829
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3271
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22476_length_3271_cov_3.906405"
                     /isolate="AMR_MDS_3118"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..977)
                     /locus_tag="P5056_04045"
     CDS             complement(<1..977)
                     /locus_tag="P5056_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62910.1"
                     /translation="MADQNNNQNLSVKRYAARIAMEGEAEKVRREAEEKAARDKEIAA
                     ELIKQKELDKAAAAERVIKDKLEEEAREKRAADEASSKIKTEVEKIKTVGAQIGTLRT
                     LKRDMDELIRNQSISLARIAMEEDARRRAGGATGQQTRNWLTLLASLILIASGLGIGG
                     YIYLNYKPSEENIPSTETAEKTIFPITIETKKELSLSGLPSEKAISEIKNAVSGSDVA
                     AGNLQRIKLTKKSDTGDAADITSQEFFTIIGSSAPGDLTRSLNKEMLLGVLNTLIGAK
                     SGVLIFNVESYQNAFAGMLNWEQGSMVKDIYETVTSNSADDFLITKKFE"
     gene            complement(1125..1694)
                     /locus_tag="P5056_04050"
     CDS             complement(1125..1694)
                     /locus_tag="P5056_04050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62911.1"
                     /translation="MDDAQNTQTQTSSNNEPTKKMEAILAMEGEEGRARREKEEQEKK
                     EAKMIADLEAEKVELNNKRTNLSKSKEELELHWIDLSDKKTALQEILNPILENEANLE
                     KNVQSKNQEEHATDDPIQRQRFEKERQALEAEREKLEKEKWGIEDKMGEIDKEMNDNK
                     SKYQELLREEYSIIDKISEIDKSIESVRK"
     gene            1829..2749
                     /locus_tag="P5056_04055"
     CDS             1829..2749
                     /locus_tag="P5056_04055"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRY62912.1"
                     /translation="MNDSISELKKQFLEYLEIEKGRSLKTVENYDRYLSRFFEFIKAK
                     SAKDITEDNVRQFRLWLNRRPGSERGLGMKKNSQNYHLIALRVFLKYLMKRKVSSLSP
                     DQIELAKTSERHLDLISQDELKRLIEAPNGGSLADLRDRAIMELLFSTGLRVSELASL
                     SRDSVDLKKDEFSIRGKGGKIRVVFLSEEAKSAIKAYLDKRGDVDDALFVRMDRDPGK
                     ISNSMAITPRSIERMIKKYAIKAGISKKVTPHVLRHTFATDLLQNGADIRSVQVMLGH
                     SNISTTQIYTHVSDKQLKEVHKNFHNKDRG"
     gene            2791..>3271
                     /locus_tag="P5056_04060"
     CDS             2791..>3271
                     /locus_tag="P5056_04060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62913.1"
                     /translation="MQIEIFYLGAGFVVGFILARLFWGSCSRCGNKDIAEKDFSQSVI
                     HELRAYITNLSWIFDKLQEKGMSSYTDDEYKALSLGKSTILNANSLINDTLSAISVGR
                     AEARFRFTLNDINKVIENIVSEYQLLAKEKGIALNFNSSAVPIPLFFFDSSQIYIAAH
                     "
BASE COUNT          835 a          673 c          632 g         1131 t
ORIGIN      
        1 tcctcaaatt tttttgttat caggaagtcg tccgcggaat ttgaggttac tgtctcatat
       61 atatctttga ccatagaccc ttgttcccag tttaacattc ccgcgaaagc attttggtaa
      121 gattcaacgt tgaatataag cacgccgctt tttgctccga tcaaagtatt caaaactccg
      181 agcaacattt ctttgttcaa cgagcgtgtc aggtctccgg gcgcggatga gcctattatt
      241 gtgaagaact cctgggatgt tatatcggcc gcatctcccg tgtctgactt tttcgttaat
      301 ttaattcttt gcaggtttcc cgccgcgaca tcgctaccgg aaacagcgtt ctttatttca
      361 gagattgctt tttccgaagg gagaccgctt aaagataatt cttttttggt ttctatcgta
      421 ataggaaaaa tagttttttc cgcagtctct gttgagggga tattttcttc tgaaggtttg
      481 taatttaaat aaatatatcc gccgattccc agtccggacg caatcaatat taagctagcc
      541 aataacgtta accagtttct agtttgttgc ccagtcgctc ctccggccct tctcctcgcg
      601 tcctcttcca tcgcaattct ggccagtgat atgctctgat ttctgataag ctcatccata
      661 tctctcttga gtgttctcaa tgttcctatc tgagctccga ctgtttttat tttttctact
      721 tcagttttta tttttgaaga agcttcatct gcggctcttt tctcccttgc ctcttcttca
      781 agcttgtctt taatgactct ttcggccgcg gccgctttat ccagttcttt ttgtttgatc
      841 agttcggccg caatctcttt atctctggcc gctttttctt cggcttctct tcgcaccttt
      901 tcagcttcgc cttccatggc gatcctggcc gcgtatcttt tgacagataa attttgattg
      961 ttgttttgat ccgccattta ccctgtttag taaaaaatga ctaataacaa attaattacc
     1021 cttctataaa ttataattat taaacccaaa cctaattata caatgaacgt catttttaaa
     1081 tttttattta ttaaaaaata aaaattttac tgaacagggt taatttattt cctgacgctt
     1141 tctatacttt tgtcgatttc gctaatcttg tctatgatag aatactcttc tctcagaagt
     1201 tcttggtatt tggacttatt gtcgttcatc tctttatcaa tttctcccat tttgtcttct
     1261 atcccccatt tttctttttc taatttctct ctttcggcct ctaacgcctg tctttctttt
     1321 tcaaacctct gtctttgtat cgggtcgtcc gtcgcgtgct cttcctgatt cttggattga
     1381 acattttttt ccagatttgc ctcgttttcc aaaataggat taagaatttc ttgaagggcc
     1441 gtcttcttgt cggaaagatc tatccagtgt agttcgagct cttcttttga tttagagaga
     1501 ttggttcgtt tgttgtttaa ttccactttt tccgcttcta ggtctgcgat cattttcgcc
     1561 tctttctttt cttgttcctc cttttctctt ctggcccttc cttcctcccc ctccatggct
     1621 aaaatagctt ccattttttt ggtgggttcg ttgtttgaag aagtttgagt ttgtgtgttt
     1681 tgtgcgtcgt ccattcgttt tattttttag tttaactata caaattttaa cacaaaaaac
     1741 tttaatcggc caaagtcaat accgcatttt tgcgacaaaa tacagttatc gcctcgttat
     1801 ggtgtggggg cgtgctataa ttaagtttat gaacgacagc atatcagaat taaaaaagca
     1861 gtttttggaa tatcttgaaa tagaaaaggg gcgcagtttg aagacggttg agaattacga
     1921 ccgttatttg agccgttttt ttgaatttat taaagccaaa tcggcaaaag acataacaga
     1981 ggataacgtt cgccagttta ggctgtggtt aaataggcgc cccggctctg aaagaggcct
     2041 cggaatgaag aaaaatagcc aaaattatca tcttattgcg cttagggtgt tcttaaaata
     2101 cttaatgaag agaaaagtgt cctctttgtc ccctgaccag atagaactgg ccaaaacaag
     2161 cgagagacac ctagatctta taagccaaga tgaattaaaa aggcttattg aagccccaaa
     2221 tggcggtagt ttggccgatt tgcgcgatag ggccataatg gagcttcttt tttccaccgg
     2281 actccgtgtt tctgagctgg cttccctttc ccgcgatagt gtcgatctaa aaaaggatga
     2341 attttctata cgcggtaagg gcggtaaaat aagggtggtt ttcttgtcag aagaagctaa
     2401 gtccgcaata aaggcctatc tggacaaaag aggagacgtg gatgatgcgc tatttgtaag
     2461 gatggatcgt gacccgggca aaataagcaa ttctatggcc ataacaccgc gatccataga
     2521 aagaatgatc aaaaagtatg caattaaggc cggtatttca aaaaaagtta caccgcacgt
     2581 tttaaggcat acgtttgcta ctgatttatt gcaaaatggg gcggatataa ggtccgttca
     2641 ggttatgttg ggccattcta atatttctac gacacaaatt tatacccacg tatcagacaa
     2701 acagctgaag gaggtccata agaattttca caacaaagac agaggataac ctatatatta
     2761 tttttcttta aataacatat aatagtgtat atgcagatag agatttttta tctaggtgca
     2821 ggttttgtgg tggggtttat cctggccaga cttttttggg gttcatgttc taggtgcggc
     2881 aataaagaca ttgccgaaaa ggatttcagt cagtcggtca ttcatgaatt acgtgcctat
     2941 atcactaatt tgagttggat attcgacaag cttcaggaaa agggcatgtc ttcttacaca
     3001 gacgatgaat ataaggcact ttctttgggc aagagcacga ttttgaacgc caacagcctt
     3061 attaacgata cgctttccgc tataagcgtg gggcgcgcag aggccagatt tagatttacg
     3121 ttgaatgaca taaataaggt tatagaaaat attgtttccg aatatcaatt attggctaaa
     3181 gagaaaggaa tagcccttaa ttttaatagt tccgcagtcc ccatccccct ctttttcttt
     3241 gatagttctc agatttacat agcggcacac g
//
LOCUS       DAOTEX010000074         3210 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3118
            NODE_23126_length_3210_cov_4.241204, whole genome shotgun sequence.
ACCESSION   DAOTEX010000074 DAOTEX010000000
VERSION     DAOTEX010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868142
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 883
            CDSs (total)                      :: 833
            Genes (coding)                    :: 829
            CDSs (with protein)               :: 829
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3210
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23126_length_3210_cov_4.241204"
                     /isolate="AMR_MDS_3118"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="P5056_04065"
     CDS             complement(<1..445)
                     /locus_tag="P5056_04065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62914.1"
                     /translation="MKLYEASWYKCPHDGESSGKHHQAGHVATSPPGKDGEMMGCIYR
                     RGKKYWIKYYRHGKQFAESSHSDKKEIAIRMLHLREAEIFQGKMPGICFDKVSFEELV
                     EDLLADYKINGKRSLDRAKLAVVHLERVFKGMKVAYIDTARITRYI"
     gene            complement(752..1009)
                     /locus_tag="P5056_04070"
     CDS             complement(752..1009)
                     /locus_tag="P5056_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62915.1"
                     /translation="MSIDTGPLTMDQIDMLRREKLALSSLADKQRAFCEEYCRSFDKL
                     QALKAGGYWTPKSAKAGGSQAALIRKSYDKKIAFISNITNG"
     gene            complement(1869..>3210)
                     /locus_tag="P5056_04075"
     CDS             complement(1869..>3210)
                     /locus_tag="P5056_04075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62916.1"
                     /translation="AVLMRDFADSLTDEEKQFCRSVIVEYAGRPLMEEKYSYQISDGV
                     EHATRSLPLLMKYLPEGKNDIKMLLFLLLLNPWPQVSTFATSAVLHDLWEISPEDAQS
                     ILLGYLTLEPKYRALAAEIRKQNYKKQIYDYSKYEVQKEFCARYEKELDKVSANTIRY
                     EDVGNLKKLDLRALNTAFELIPLETNDNVHRLLLDGIFPIFAAKIMSNERQHGHRRQN
                     DEEQLDYEVQHRFCNKFAYFVLTSKKEDIPKYLKPFVDNFSDSRETADFLQEFITVED
                     FLNQYEEFWTVWDILYQPVLDICKKGYFGYHGAEIIHNYLLALPWKETAKEWHSLLDR
                     EKYFLSRVAQEMGGYPPAFYSLCKLLNDIGSRFLEDGIFWLCNIIQRTPTLVSDKLAV
                     DTIYYLENLIRKYVLTNRPKIKRTLKVKDAFMVLLNFLIERGSVTGYLLREDIL"
BASE COUNT          848 a          714 c          648 g         1000 t
ORIGIN      
        1 cgatgtagcg tgttattctt gccgtgtcga tataagccac tttcattccc ttgaaaaccc
       61 tttctaggtg tacaacggct agctttgcac ggtctagtga acgtttccca ttaattttat
      121 agtccgccag taaatcttcc accagctcct cgaatgaaac cttgtcaaaa caaattccgg
      181 gcattttacc ttgaaaaatc tcggcctcac gaagatgcaa cattcgtatt gcgatttctt
      241 tcttgtcact gtgcgaggat tctgcaaact gttttccgtg tctgtaatat tttatccaat
      301 actttttacc tcgtctgtaa atacatccca tcatttcacc gtcctttccc ggcgggctcg
      361 ttgcgacgtg ccctgcctga tgatgttttc cagacgattc cccatcatgg gggcatttat
      421 accacgaagc ttcatacaat ttcacctttt aatgataaac tgacttattc ttttcaatga
      481 atccatctag atcagttata tcgatataaa tcttgcgcga acccggctgt ttaactactg
      541 gaattatctg tttccagatt aaatctctca tgccccactc agaccggcct agatagttac
      601 ccgactcctt aagcgtaaaa aggcgtttcc gcacgggatt gtgtatcccc tgcggcttct
      661 gtatttcaat gcctgtctgt atttgttttg ccatgtagct attttgtcac tatcgtattt
      721 agatttagat taaccactcg tatattgaca tctaaccatt tgtaatattg gatataaatg
      781 ctattttctt gtcataagac ttcctgatca gggcagcctg gcttccgccg gccttggcgg
      841 atttcggggt ccagtatccc ccggccttca gagcctgtaa cttatcgaaa gacctgcagt
      901 attcctcgca gaacgctctc tgcttgtcgg ccagggagga aagcgccagt ttctcccgcc
      961 gcagcatgtc gatctggtcc attgttaacg gcccggtatc tatagacatt tgcacctcgc
     1021 tcagggaaat aagaaaattt atttttacat tgcttgacat tttcgaataa gtcaagtgct
     1081 tgaaaaaata aatattcctc tccgtgagcg aggcggagcg actccacagt tggacgcaat
     1141 tgataggatc gctccgtttt ttgtttgatt cgacattttt gtcgaacttg ggtcgtaata
     1201 gggtaaataa cgtccctcta tataaggaaa gttttttatg tatttttttt tgaacagtaa
     1261 ggaatactca aaaaagtatg agatttgatt attattgtta ttaataaatg agaaacagat
     1321 acttacacaa caagtgttac tattacacag gtattatggt tatttttaag tccccataat
     1381 ccggaagata aaagataaaa gaatttttct gtaaaaaaaa tgttaaaaaa atcttcctta
     1441 tatagagtgt gcgctgctct gtggagtaga tccaacccgc ctgatccaaa aattccaaaa
     1501 tcctgaaaaa aattgtgcgt tttgtaagag ggtttacccc tctacttcct gaagctacga
     1561 taattgaccg ccaccgggtt gtcccaggaa cggtgaataa tccgcatcgt gtcatcgctt
     1621 gctgttcagg gctgcttcgt tgttcagcta cagagagaag ccggtttggt gctggcgata
     1681 gttttggtcg gtgtggtgcg tctaacttcc tgtgctcgat aaaacggaga tcgatatgga
     1741 aatccacact ttcaaataca agtacgcact ttgaccaacc tgcctcgaaa attgctttcg
     1801 gattgaaagg cacaattttt ttgaggcaat tatttcacgt gaatgtgata tttacaattt
     1861 cctgtactct acaaaatatc ttccctcagc aaataaccgg taacggaacc tctctctatc
     1921 agaaaattca gaagtaccat aaatgcatct ttaactttga gcgtcctttt gattttcggg
     1981 cgattggtta gcacatattt tcgaatcaaa ttctcaaggt agtaaattgt atccactgct
     2041 aatttgtctg agacgagcgt tggcgttcgc tgtatgatgt tacatagcca gaaaatgcca
     2101 tcctcaagaa acctgctgcc tatatcattg agaagcttgc agagtgagta gaaggcggga
     2161 ggatagcctc ccatttcctg tgcgactcta ctcagaaagt atttttctct gtcaagcaga
     2221 gagtgccatt ctttggcagt ttccttccac ggcaaggcca aaagataatt gtgaataatc
     2281 tctgccccgt gatagccgaa gtagcccttc ttgcagatat caaggaccgg ctgatagagg
     2341 atatcccaga ctgtccagaa ctcttcatac tggttgagaa aatcctcaac agtgatgaac
     2401 tcttgcagaa aatcggcagt ttccctggaa tcgctgaaat tatcgacaaa tggtttgaga
     2461 tatttcggga tgtcttcttt cttggaggtc aacacaaaat aggcgaactt attgcagaac
     2521 ctgtgctgaa cttcatagtc aagctgctct tcatcatttt gcctccggtg tccatgctgt
     2581 cgttcattcg acattatttt agcggcaaat attgggaaaa tgccatccaa gaggaggcga
     2641 tgtacgttat cgttagtttc cagaggtatt agctcaaaag cggtattcag tgctctaaga
     2701 tcaagcttct ttaaattgcc cacatcctca tagcggatgg tgttagcgct aactttatcc
     2761 agctctttct catatcgcgc gcaaaactct ttttgtacct catatttgga gtaatcgtag
     2821 atttgcttct tgtagttttg ctttcttatc tctgcggcca aagccctata ctttggctct
     2881 aaggtcaaat atccaagtaa tatcgattgc gcgtcttcgg ggcttatttc ccataaatca
     2941 tgcaggactg cacttgtcgc aaaggtggat acctgtggcc agggattgag aagcagcaga
     3001 aatagaagca ttttgatatc attctttccc tctggcaggt acttcataag taagggaaga
     3061 cttcgtgttg catgctccac gccatcagaa atttgatagg aatatttctc ctccataaga
     3121 ggccggccgg cgtattcaac aatgacgctt cggcaaaatt gcttttcttc atcagtcaga
     3181 gagtcagcaa aatctctcat cagaacagcg
//
LOCUS       DAOTEX010000075         3093 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3118
            NODE_24544_length_3093_cov_5.378868, whole genome shotgun sequence.
ACCESSION   DAOTEX010000075 DAOTEX010000000
VERSION     DAOTEX010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868142
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3093)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3093)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 883
            CDSs (total)                      :: 833
            Genes (coding)                    :: 829
            CDSs (with protein)               :: 829
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3093
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24544_length_3093_cov_5.378868"
                     /isolate="AMR_MDS_3118"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1426)
                     /locus_tag="P5056_04080"
     CDS             complement(<1..1426)
                     /locus_tag="P5056_04080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62917.1"
                     /translation="MGQKSFKIFIVISIIFFGFSSSAFGFQNSGDFDLFDIKNEVLAG
                     VRQDLSVFCKGALYEVSDRIETAFKSLNQQVSTNIDGENSFTVFALDNIQYDLAQTSA
                     AVGNIFERAANYFGTGIENLVKNISSAEPIVFDGGATSGTITDTEALQIEKEKFNFIR
                     RDEMGGLEGGYTKLPGTTSVAPVAEKTPTETVVRETREVVYAGGVNVTKPLVSSLGFY
                     TKEELDSILKNYDQTTRNDLLSKINNLSAVNNYYYAGNTNAIALTNKIDSLTGVSLTN
                     ATVSGISGLTDSDIPDGITVSGYLPLSGGNVTGNLGVGTTSPYAPLSVDGEVAASFLT
                     TSSSTATSTFAYGINLKAGCFAINGVCVTGDSSVGGSWSTTSADYYINSSSTIPKSYT
                     ENVFTASTTFGAQTNMQFASSSALTVTGNSYLGNISSGVWNGTEIGVAYGGTGTTTAP
                     LYGQMLLGNNIGGYSYVATSSLGLL"
     gene            complement(1635..2294)
                     /locus_tag="P5056_04085"
     CDS             complement(1635..2294)
                     /locus_tag="P5056_04085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62918.1"
                     /translation="MPKNSLIIFAIISCFVTSTVANAYVASSTNFRIETDSISYGGGM
                     STSTNFDMQDTLSNMFLGTGTSTQYRLDTGAIAMTVSTITMTDPGSLTLTGEINSLDG
                     GSAEGSVSVNVMTDNPGGYKLTLSGTTLSSGADSFASFSGPAAWSVSDGASAFGFSTD
                     LSTWNGVAASETDVVIENSNNQPSGTDTTITFRAESQKKNQTQSDGRYSSTITLTATV
                     L"
     gene            complement(2281..>3093)
                     /locus_tag="P5056_04090"
     CDS             complement(2281..>3093)
                     /locus_tag="P5056_04090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62919.1"
                     /translation="SFGFSVVKHIDVPVTKSFSIGPSKLEFQVDEGNIVKKELHIFNA
                     SGVDSLFNISFEDIFDKATVTPGVPKISKYLSVDNNNFLLKHGEEAVIDVFVKVPELN
                     TDKFVGGAVLVSRRDASSDATKISARLGALVFVDIGKMAKRSGYVTNFKYLEDGAFDI
                     VFKNTGEAKLNPYGFIEFRNILGDYSGRSVVSPWYVLPGTERTLKINWERPSVFSPVY
                     NAHLVMYPGFGGPEGTENISIRVYNFCAISAYVILSLLFIIIFIKYIRHAKK"
BASE COUNT          940 a          708 c          591 g          854 t
ORIGIN      
        1 tgagtagacc caaagacgag gtggccacat aagaatatcc gccgatatta tttccgagga
       61 gcatctggcc atacaaagga gccgttgtgg tgcccgttcc gccgtaagcg actccgattt
      121 ctgtgccgtt ccaaacaccg ctagaaatat ttcccagata tgagttgccc gtaactgtca
      181 gggcagacga agaggcaaac tgcatatttg tttgagctcc aaatgtcgtg ctggcggtaa
      241 acacgttctc tgtgtagctc ttcgggattg tcgaggatga attgatgtaa tagtccgcgg
      301 atgtcgtgga ccacgaaccg ccgaccgagc tgtcgcctgt tacacaaact ccgtttatcg
      361 caaaacatcc cgccttaaga tttataccat aagcaaaagt tgaagtagct gtcgaagatg
      421 atgtggttaa gaatgacgcg gcaacctccc cgtcaacaga aaggggcgca taaggagaag
      481 ttgttcctac acccaaatta cccgtaacat tgcctccgga cagaggaaga tatccggaaa
      541 cagtgattcc gtccggaata tccgagtcag taagcccgga aataccgctc actgttgcgt
      601 tcgtcaaaga gacgccggtc aaactatcta tcttgtttgt aagggcgatg gcgttagtgt
      661 tgccggcata ataataatta ttcacggcgc taagattgtt tattttagaa agaagatcgt
      721 ttcgtgtagt ttgatcataa ttcttcaaaa ttgaatccaa ttcttctttg gtataaaaac
      781 ccaaagatga aactaacggt ttggttacgt ttaccccgcc ggcataaaca acttctcttg
      841 tctccctaac gactgtttct gttggcgttt tctccgccac aggcgcaacg cttgtagttc
      901 cgggaagctt tgtataaccg ccctccaatc cgcccatctc gtctcttctg ataaaattaa
      961 atttttcttt ttcaatctga agcgcttcag tatctgttat ggttccacta gtcgctccac
     1021 cgtcaaacac aatcggctca gcggaagaaa tatttttgac taaattttct atgcccgttc
     1081 caaaataatt tgcggctctt tcaaaaatat tacccacagc cgccgatgtt tgagccaaat
     1141 catactgaat attatccaaa gcaaaaacag taaaactatt ttcaccatct atgttcgtgg
     1201 agacttgttg gttaagagat ttaaaggcgg tttcaatcct gtctgaaacc tcgtataagg
     1261 cgcctttaca aaaaacagaa agatcttgcc ggacaccggc aagaacttca ttttttatat
     1321 cgaagagatc aaaatcccct gaattttgaa aaccaaaagc agaagaagaa aaaccgaaaa
     1381 atattatcga gattactata aaaattttaa aggatttctg acccaaaatc atttttaaat
     1441 ataagccgac ttcattgcga taggaaaata aaacctccgc caggccttat ctataatttt
     1501 attacaaaaa taaatattta agaaacgaaa agagacaaaa aaatggggat aactttctgt
     1561 ttttgcggct caaaataaag accgcttcct gtttcaataa aaaggaagcg atcttatgat
     1621 attttttcaa aaaattacaa aacagtagcg gtcaaggtaa tggtagagct atatcttccg
     1681 tctgactggg tttggttttt cttctgagac tctgccctaa atgttatcgt tgtgtctgtt
     1741 ccagacggct gattgttgga gttttctatg accacgtcgg tttcactagc cgccacccca
     1801 ttccatgtgc taagatctgt cgaaaatcca aaagcagatg cgccgtccga cacgctccat
     1861 gccgccggcc cggaaaagga agcgaaacta tccgcacctg atgacaacgt ggttcccgac
     1921 aacgtgagct tatatcctcc ggggttatcg gtcataacat taacagacac agaaccctct
     1981 gcagaaccgc catctagaga gtttatctcc cctgttaaag ttaggctccc ggggtctgtc
     2041 atcgtgattg tagaaacggt catcgcaatc gctccggtat caagtcgata ttgtgtggat
     2101 gtccccgttc ccaaaaacat attgcttagc gtatcctgca tatcaaaatt cgtggaagta
     2161 gacatccctc ctccataaga aatggagtct gtctctattc tgaaatttgt gctacttgcg
     2221 acatatgcat tcgcgaccgt gctagtgaca aagcaggaga taatcgcgaa aattattaaa
     2281 ctattttttg gcatgtctta tatattttat aaaaataatt ataaacaata acgataaaat
     2341 aacataagcg gaaatagcgc agaaattata tactcttatc gaaatattct ctgtgccctc
     2401 cggacctcca aaaccaggat acataactaa gtgggcatta taaacaggag aaaagactga
     2461 cggcctctcc cagtttatct taagagttcg ttcggtgccg ggaagaacat accacggaga
     2521 gacaacgctg cgccccgagt aatctcccaa aatatttcta aactctataa aaccataagg
     2581 gttcagtttc gcttctcctg tatttttgaa aacaatatca aacgccccat cctctaaata
     2641 tttaaaatta gttacataac cggacctttt cgccatcttg cctatatcaa cgaacacaag
     2701 cgcgccaagt ctggccgaaa tttttgtagc atccgatgac gcatcgcgac gagataccaa
     2761 taccgcaccg cccacgaact tatccgtatt caactccgga acttttacaa atacatcaat
     2821 aaccgcctcc tctccgtgct taagtaaaaa attattatta tcaacggata gatatttact
     2881 tatcttggga acacccggcg taacggttgc cttatcaaaa atatcttcaa aactaatatt
     2941 aaaaagagaa tctactcccg aggcgttaaa aatgtgtaac tcctttttta ctatgtttcc
     3001 ttcgtctact tgaaattcta atttagaagg acctatggaa aaactttttg tcacagggac
     3061 atctatgtgc ttcacgacgc taaacccaaa aga
//
LOCUS       DAOTEX010000076         3032 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3118
            NODE_25282_length_3032_cov_3.773262, whole genome shotgun sequence.
ACCESSION   DAOTEX010000076 DAOTEX010000000
VERSION     DAOTEX010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868142
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 3032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 883
            CDSs (total)                      :: 833
            Genes (coding)                    :: 829
            CDSs (with protein)               :: 829
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3032
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25282_length_3032_cov_3.773262"
                     /isolate="AMR_MDS_3118"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1401
                     /locus_tag="P5056_04095"
     CDS             <1..1401
                     /locus_tag="P5056_04095"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HRY62920.1"
                     /translation="VPVDGKIVFGKASMNEASVSGESLPIDKVVGDEVFSSTLNESGF
                     IKIRATGIGKNSTVERIAELIKNASAHKSKQEKLADNFAEVFLPALLILGCVTYYFSQ
                     DIKMVASLFLVACADDMAVAIPLAMTASLGRAARRGVIIKGGQWLDALANMKTIVFDK
                     TGTLTYGTLAVQGIHLEEGVSEEYFWKMVGSAEKYSEHPIGRAIFKEANKKISEITDP
                     DTFERHESKGVFASFGGDTVAVGNENLFPEIGIVLDKKVRDILVRERLEHKITTALVY
                     INKKFSGVITVADVVHPEAKRSVESLRNLGVENIQIFTGDNEEITKTVSSNLGINNFN
                     AKMVPETKLLELEKMSKVGVVGMVGDGINDAPSLARADVGIAMGNGSSAVAVEAADVI
                     ILTNNLNRIPEMIRLGRKTQLIIRWNMFIWAISNIVGFALVFTGVIGPVLAAFYNFAT
                     DFFPLINSSRLFKEKI"
     gene            complement(1443..2009)
                     /locus_tag="P5056_04100"
     CDS             complement(1443..2009)
                     /locus_tag="P5056_04100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62921.1"
                     /translation="MLKRIVSREQSHTDKITDLHSNIWGDFGDIRGSVKNIYGDLRIH
                     KISGDTSKIKGDIGGITGDVSNVSGEVTSIIGDLSNLSGEVSGIRGDVSGIIGDAGNI
                     KGNVTNIRGNVSKIQGDVSRIIGDVGDLSGDVSRLSGNITNLSGYVSNIFGNTTNLRG
                     DVSNIFGNVNGIEGDATNLHGNIEEIAK"
     gene            2153..>3032
                     /locus_tag="P5056_04105"
     CDS             2153..>3032
                     /locus_tag="P5056_04105"
                     /inference="COORDINATES: protein motif:HMM:NF024923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HRY62922.1"
                     /translation="MNKKIIYAVVALVVVVLGYLYFSSGGNGSYEYVTIEKRDIVQEV
                     SVTGKVKPVSSAMLAFDRLGKVVRFNVDVGNHVSAGDVLASLDSSELAANLLQAEASV
                     RVQEAKLDELKRGARTEDLEVSYAERDSATTALVDAKKGLVEKINDAYTKSDDAIRNR
                     VDRMFTNPRSANPELVFIVADATLSDQIKAERVILEGVLNNWNSSLGSLSSGADLGKY
                     VSKAYANLEKIKSFLDKMALAVSGLTTNATLTQTTLDTYKSETATSRTNINSAISALV
                     AADEKMRAADSALRVAS"
BASE COUNT          846 a          568 c          737 g          881 t
ORIGIN      
        1 gtgcccgttg acgggaaaat tgtttttggg aaggcttcta tgaatgaagc atctgtaagc
       61 ggggagtccc taccgattga taaggttgtc ggggatgagg tttttagctc cacgctcaat
      121 gagtccgggt ttataaaaat tcgcgccacg ggaatcggta aaaactcaac agttgagaga
      181 attgcggagc taatcaagaa tgcctccgcg cataaatcaa aacaggaaaa attggcggat
      241 aattttgctg aggtatttct cccggctctt ctaatcctcg gatgtgtaac atattatttc
      301 tctcaagata taaagatggt ggcgtcactc tttttggtgg cttgcgctga cgatatggcc
      361 gtcgcgatac cccttgcgat gacggcatct ttgggtcggg ccgcaagaag gggcgtaata
      421 ataaaaggag gccagtggct tgatgcattg gccaatatga agactattgt ttttgataaa
      481 acaggcacac tgacttatgg cacgctagcc gtgcagggta ttcatctaga ggaaggtgtc
      541 tctgaagaat atttttggaa aatggtcggt tctgcggaaa aatattccga gcatccgata
      601 ggcagagcca tattcaaaga ggcaaataaa aaaatatcgg aaattacgga ccccgacacc
      661 ttcgagaggc atgaaagcaa gggtgtcttt gcgtcttttg gcggtgacac tgtggctgtc
      721 ggtaatgaga atttgtttcc ggaaatagga attgttcttg ataaaaaagt gcgggatatt
      781 ttggttagag aaaggttgga acacaaaatc actacagctt tagtgtatat aaacaaaaaa
      841 ttttcaggag ttataacggt tgcggatgtt gttcacccgg aggctaaaag aagcgtagag
      901 tcccttagaa atttgggggt ggaaaatatt caaattttta ccggggataa tgaagagatt
      961 acaaaaacag tttcttcgaa cttgggtata aataatttta atgcaaagat ggtcccggaa
     1021 acgaagctcc tagaattaga aaaaatgtct aaagtcgggg tagtggggat ggtgggcgat
     1081 ggaataaatg atgccccaag cctcgccagg gccgatgtcg ggattgctat gggaaacggc
     1141 tcttctgcgg tagcggtaga ggcggcagat gtaataattc ttacaaataa tttaaataga
     1201 attcctgaaa tgataaggct gggtagaaag actcagttaa taatacgttg gaatatgttt
     1261 atttgggcaa tctcaaatat tgtcggtttt gccctagtat ttacaggagt aatcggccct
     1321 gtccttgccg ctttttataa ctttgcgacg gattttttcc ctctcataaa ctcttcccgt
     1381 ttgtttaaag aaaagatata aaacaaaaac gaccctgatg agggtcgttt ttgatgtgta
     1441 atttattttg cgatttcttc tatgttgccg tgtaagttag tggcgtcacc ctctatcccg
     1501 ttaacattcc caaaaatgtt gctaacgtcg cctctaagat ttgtcgtatt gccgaagatg
     1561 ttgcttacat atcccgagag attggtaata tttccggaca atctgcttac atcgccggaa
     1621 agatctccca catcgccgat tattctgctg acatctccct gtatcttgct gacgttccct
     1681 cttatgtttg taacgtttcc tttgatattg ccggcgtctc cgataatacc gcttacgtct
     1741 ccacgaatgc cgctaacttc tcccgatagg tttgagaggt ctcctattat actggtaact
     1801 tctccggaca cattgctgac atcgcccgtt attcctccga tgtcgccttt tatttttgag
     1861 gtatcaccgc taattttgtg aatcctgagg tcgccgtaaa tattctttac gctaccgcgt
     1921 atatcaccaa aatctcccca aatattacta tggaggtctg taatcttgtc tgtgtgggat
     1981 tgctccctgc tcacaattct ctttagcatc gctttgtttt atgtttaagt gttaatttta
     2041 tgtttattaa tatatcacaa ttgcctcatt tatcaattcg ttggggattg tgggaatagc
     2101 ggtagttctt tgtcgggagt ataatatata ggtataaatt ttgtttaaaa atatgaataa
     2161 aaaaataatt tacgctgttg tggcgctggt tgtggtcgtc ttggggtatt tgtatttctc
     2221 gagcggagga aatggttcat atgaatacgt tactattgag aaaagagata tagtccagga
     2281 ggtcagcgtt accggtaagg taaaaccggt ctcaagcgcc atgcttgcct ttgataggct
     2341 cggcaaggtt gtcagattta atgtagatgt ggggaatcat gtatcggccg gcgatgtttt
     2401 ggcttctttg gatagcagcg agcttgccgc gaacctattg caagctgagg ctagcgtaag
     2461 ggttcaagag gcgaagttgg atgagttaaa aaggggagca agaacagaag acctcgaggt
     2521 gtcttatgcc gagagagata gcgccactac ggctttagtg gacgcaaaaa agggattggt
     2581 agaaaagata aacgatgcat atacgaaatc agatgacgca ataagaaata gggtagacag
     2641 gatgttcacc aacccaagaa gcgccaaccc cgagctggta tttattgtcg cagacgcgac
     2701 cttgtctgat caaatcaagg ccgaaagagt tattttggag ggcgttttga ataattggaa
     2761 ttcttctctc ggaagcttgt cttccggtgc tgatctcggc aaatatgttt ctaaggctta
     2821 tgctaatcta gaaaagataa aatctttttt ggataaaatg gcgcttgccg tgagcggatt
     2881 aacgacaaac gcaactctga ctcagaccac cctagataca tataaatcag aaacagcaac
     2941 atcaagaact aatataaatt ccgcaatttc agcgcttgtc gcggccgatg aaaaaatgag
     3001 ggcggcagat tcggcgttaa gggtggcctc ac
//
LOCUS       DAOTEX010000077         2985 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3118
            NODE_25862_length_2985_cov_4.193857, whole genome shotgun sequence.
ACCESSION   DAOTEX010000077 DAOTEX010000000
VERSION     DAOTEX010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868142
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 883
            CDSs (total)                      :: 833
            Genes (coding)                    :: 829
            CDSs (with protein)               :: 829
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2985
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25862_length_2985_cov_4.193857"
                     /isolate="AMR_MDS_3118"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(10..390)
                     /locus_tag="P5056_04110"
     CDS             complement(10..390)
                     /locus_tag="P5056_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62923.1"
                     /translation="MDSKITNIIIGIVAVIILVAAFMYSGVGTTSTEGLSTEQATGGS
                     EIGAFLRQISAIKTISLDRGVFENKVLANLKDQSKEIEKEVPQKKNPFAPISRQSASL
                     QQTAGASSQTAVVETAPVAAPLTD"
     gene            complement(390..929)
                     /gene="pilO"
                     /locus_tag="P5056_04115"
     CDS             complement(390..929)
                     /gene="pilO"
                     /locus_tag="P5056_04115"
                     /inference="COORDINATES: protein motif:HMM:NF016253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HRY62924.1"
                     /translation="MNYLISLTLITISFFLSLFYTSPLLSDIDALGAERNSLSASLSE
                     SKGLFEVYRQKQAVYNNFTDTEKERINKVLPDSIDNVKLVIDIDDIASKYNMKIRNID
                     LKTEKVGEGEMTSNNPYGTATLRFTVSSNYTNFRSFLKDLEDSLRLVDISSLSFTASD
                     KNTDEYNIELKTYWLKETI"
     gene            complement(935..1573)
                     /locus_tag="P5056_04120"
     CDS             complement(935..1573)
                     /locus_tag="P5056_04120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62925.1"
                     /translation="MIGDIQRGGVQASFIPKKPLISSGQRERVGFSAILNFISIIIFV
                     IALSVFGGGTFYKTVLKSSIANIKADLEKVQNNFKEEDSAMKEMIRFDAKLNNMNALL
                     DSHISLQNLFGFLEDSTMSKLRYTEFAYTNKGNGSADLKLGGEAKSYSDIALQAEQFV
                     NSKMTGNKDFSNIIFSDFNPGLTGNVVFKATANIDPEFIHYKNLKALQGGIE"
     gene            complement(1612..2733)
                     /gene="pilM"
                     /locus_tag="P5056_04125"
     CDS             complement(1612..2733)
                     /gene="pilM"
                     /locus_tag="P5056_04125"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HRY62926.1"
                     /translation="MAFSDFLKKIGIGSTPDSVVGIDIGSSSIKVVQLKKERGVAKLE
                     TYGEVALGPYAGMAVGQSTSLPPEKLVEAIKDLFKEVNINATSASLSVPLRASLLKLI
                     EVPKIEERNLDQMVSLEARKYIPVPITEVALDWMIVPSHEYSETQTSSMPTAEGEARK
                     IPKMEVLIVAIHKSIISAYGEIINKLGLKATPLEIETFSASRANLSNELNAVAILDLG
                     ASASRLLIVDYGVAKASHVISKGAQDITLSLQKSLGLSFAEAEEAKRRLGAIGKVEGG
                     DLNAVISPTLEYIFYEAQKILIAYQKKYSRPITKVILTGGGAQLKGIVVLAKKEMEVE
                     VEIGNSFKKIEVPAIMEKALAENGSGFGVAAGLALRGLQ"
BASE COUNT          800 a          686 c          604 g          895 t
ORIGIN      
        1 taaaaaatat taatccgtta acggtgcggc tacaggagcc gtctctacaa cagcggtctg
       61 gctagaggcc cctgctgttt gctgtagcga agcggattgt ctactaatag gagcgaacgg
      121 attcttcttc tgagggactt ctttttctat ctccttactc tgatctttca aattggcgag
      181 aactttattt tcaaaaactc ccctgtctaa cgagattgtt tttatagcgg aaatttgtct
      241 caagaaggcg cctatttcag atcctcccgt ggcctgctct gtagaaagtc cttccgtgct
      301 tgtagtgccc actcctgaat acataaaagc ggcgaccaaa ataattactg cgacaatccc
      361 tattattatg tttgtgattt tagaatccat tatattgttt ctttaagcca ataagttttc
      421 agctcaatat tgtattcgtc ggtattctta tcgcttgcgg tgaatgatag cgatgaaata
      481 tcaacgagtc tcaagctgtc ttcaaggtcc ttaaggaatg acctgaaatt agtgtaattt
      541 gaagaaaccg taaacctcaa agtggcggtg ccgtatggat tattgcttgt catttctccc
      601 tcgccgactt tttctgtctt aaggtcgata tttctgatct tcatgttgta tttggaggcg
      661 atatcgtcta tgtcgataac aagcttcacg ttgtctatgc tgtcaggaag cactttgttg
      721 atcctttcct tctcagtgtc ggtgaaattg ttataaacgg cctgcttctg cctataaacc
      781 tcgaagagtc ctttgctctc ggaaagagaa gcgcttagag aattcctctc tgcgccgaga
      841 gcgtcaatgt ccgataaaag agggcttgta taaaacaatg acaagaagaa tgagattgtg
      901 atcagcgtta atgaaattaa ataattcata ttttttattc tattccccct tgtagggcct
      961 taaggttctt gtaatgtata aattccggat ctatattcgc ggtggctttg aatacaacat
     1021 ttcccgtaag cccggggtta aaatcggaga agattatatt cgaaaaatcc ttattcccgg
     1081 tcatctttga atttacaaat tgttcggcct gcaaggcgat atcgctgtag ctctttgctt
     1141 cgcctcccag cttgaggtca gcggagccgt tgcccttgtt tgtgtaggcg aattccgtat
     1201 atctcagttt tgacatcgtg gaatcttcca agaatccaaa gagattctgg agagaaatat
     1261 ggctgtccaa aagggcgttc atattattca gcttagcgtc aaatctaatc atctctttca
     1321 tagcagaatc ctcctccttg aaattatttt gaaccttctc cagatcagcc tttatgtttg
     1381 caatcgaaga tttcaaaacg gttttataga aagttcctcc cccgaagaca gaaagggcga
     1441 ttacgaaaat tataattgaa ataaaattta gaattgcgga aaaaccaaca cgctctctct
     1501 gaccggaaga aatgagcggt ttctttggga tgaatgacgc ctgaacccca cccctttgta
     1561 tgtcgcctat catatttaaa tttattaata aaaattttta ataaccaaaa tttattgtaa
     1621 acctcttagg gccaacccgg ccgcaacacc aaaaccggaa ccattttccg caagagcctt
     1681 ttccataatc gcgggaacct ctattttttt gaaagaattg cctatctcta cctctacctc
     1741 catctccttt ttggctaata caacaatccc cttcagctga gctcctccgc ccgtcaaaat
     1801 aacttttgta atcggtctgg aatatttttt ctgataagca atgagaattt tctgcgcttc
     1861 ataaaaaata tattcgagcg tcgggcttat aacagcgttc aaatcgcctc cttcaacctt
     1921 accgatggcc ccaagccttc tcttcgcctc ctccgcctct gcgaaactga gccccaaaga
     1981 cttctgcaac gatagagtaa tatcttgggc acctttactg atgacatgcg aggctttagc
     2041 aaccccgtaa tcgacgataa gaagccttga agcagaagcg cccaaatcga ggatggcaac
     2101 ggcgttaagc tcgttagaaa gatttgctct ggaggcgctg aatgtctcta tttcaagagg
     2161 cgtggcctta agccccagct tatttattat ctctccatag gcggaaatga tgctcttgtg
     2221 aatagcgaca atcaaaactt ccattttagg gattttcctc gcctcaccct cggcggtagg
     2281 catcgaggaa gtctgtgtct cactatattc gtgcgaaggc acaatcatcc aatccagcgc
     2341 aacttctgtt atcggcaccg gaatatattt tctggcttcc aaggatacca tctggtccaa
     2401 gttcctctcc tctatttttg gaacctctat caatttcaaa aggctcgctc ttagagggac
     2461 agacaagctg gcggaagtgg catttatatt tacttctttg aaaaggtctt ttatggcttc
     2521 aacaagcttc tccggtggga gagacgtgga ctgcccgaca gccattcctg cgtaagggcc
     2581 gagagcgact tctccgtatg tttcaagctt ggcgacacca cgctcttttt ttagttggac
     2641 aactttaata gacgaagagc cgatatctat accgactacg ctatccggcg tagaaccaat
     2701 accaattttc tttaaaaaat ctgaaaatgc cattgttatt ttgaagacgg aaattatagg
     2761 caagagtccc ctcgcgtaaa ttgccagact tcagtagtta ttataacata gctagtcttt
     2821 aaaatttatt ttcaaaaagc cgacttatcc acaccgtagg aaggttcgga cagatttgag
     2881 acggccaaaa gaacttaagc gctcggaact cgcctgcctg ccctgcgtgc gcaggcacgg
     2941 caggcaggcc tgacggctcc cttttgggta atattacgag aaaga
//
LOCUS       DAOTEX010000078         2933 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3118
            NODE_26529_length_2933_cov_3.677554, whole genome shotgun sequence.
ACCESSION   DAOTEX010000078 DAOTEX010000000
VERSION     DAOTEX010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868142
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 883
            CDSs (total)                      :: 833
            Genes (coding)                    :: 829
            CDSs (with protein)               :: 829
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2933
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26529_length_2933_cov_3.677554"
                     /isolate="AMR_MDS_3118"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1428
                     /locus_tag="P5056_04130"
     CDS             <1..1428
                     /locus_tag="P5056_04130"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HRY62927.1"
                     /translation="TLQDNLSLALAAHPLRIEAPIPGKSLVGVEIPNKLKSIVGMGSL
                     FNTQTFQDSPHALYLALGKTVSGVPYYSNLSKAPHMLIAGTTGSGKSVTIHTIISSLL
                     YRNPPERVKFIMIDPKRVELTLYKGIQHLLTSVITDPKKAILALKWAAKEMERRYNIL
                     EEATAKDIQSYHKMVEESKKMSTGEENEEETMESMPYIVIIIDELADIMSAYPRELES
                     SIVRLAQMSRAVGIHLILSTQRPSVEIITGLIKANIPTRVALQVPSQIDSRTILDAPG
                     AEKLLGAGDMLYIGSETSKPIRIQSGYITEGEIKKLVKFLKERHQDDVPEEINISEVK
                     EGDTLASADFGSEGVDGEDEAIFNEAKKVVMSTQKASTTLLQRKLKLGYARASRIMDL
                     LEERGVIGPGDGAKPREVYMKSGDNMGGGGAVSALINEYEGAEIAEEEEKEGTDLEAT
                     MARAESTEEEAEGKEEPTEEEEEFV"
     gene            1456..1863
                     /locus_tag="P5056_04135"
     CDS             1456..1863
                     /locus_tag="P5056_04135"
                     /inference="COORDINATES: protein motif:HMM:NF020700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62928.1"
                     /translation="MRDRIRTNIKISVIGLITLAIFSYAYFETIEVLRGPIIEIKSPE
                     SGYTATNKVVEISGNVKNVSYMYLNGKPIFADTSGKFKEKVILNDGYNSVFIEAKDRI
                     GNKKSEILELIVNNNKASIGDGLARDEDALKSI"
     gene            2283..>2933
                     /gene="recA"
                     /locus_tag="P5056_04140"
     CDS             2283..>2933
                     /gene="recA"
                     /locus_tag="P5056_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HRY62929.1"
                     /translation="MAKKSAEKTEKAGKSLGANVDNAIKAIQSKFGEGAIMKLGERPK
                     VGVDAIPTGSIGIDIALGVGGLPRGRIIEIFGPESSGKTTLALHVVSEAQKKGGVCAY
                     IDAEHAMDPEYTKKLGVKIEDLLISQPDNGEQALEIAESLIRTGGIDVIVIDSVAALT
                     PKDEIEGDMGAHHVGKQARLMSQALRKMTALVAKSKTVVIFINQIRMQIGVMFGNPE"
BASE COUNT         1013 a          586 c          674 g          660 t
ORIGIN      
        1 acccttcagg acaatctctc tctcgctctg gcggcacacc ctcttcgtat agaagcccct
       61 atcccgggca agtctctcgt gggcgtagaa atcccaaata aactaaaatc aatcgtcgga
      121 atggggtcac tgtttaatac tcaaacattc caagattcac ctcacgcact atatctggcg
      181 ctcggcaaga cagtctcggg ggtcccttat tattcaaatc tgtccaaagc tccccatatg
      241 ctcatcgcgg gaactacagg ctccggaaaa tcggtcacaa ttcacacgat aatttcctct
      301 cttttataca gaaaccctcc agagagagtt aagttcatta tgattgaccc aaagagagtc
      361 gaactcactc tctataaagg cattcagcat ctcctgacga gtgttattac cgatccgaag
      421 aaggccattc tcgcgctcaa gtgggcggca aaagaaatgg aaagaagata caacattctt
      481 gaggaagcca ccgcaaaaga cattcagtct tatcacaaaa tggttgaaga gagcaaaaag
      541 atgtccacag gagaggaaaa cgaggaggaa acgatggagt cgatgccgta tattgtcatc
      601 attattgatg agttggccga tattatgtcc gcatatccga gagagcttga gtcatctatc
      661 gtaagactgg cccaaatgtc ccgagctgtc ggtatccacc taatactttc cacgcaaaga
      721 ccttcagtag agattattac gggtcttatc aaggcgaaca tccctactag agtcgccctc
      781 caggtgcctt ctcaaataga ctctagaaca atcctagacg ctcccggtgc ggaaaagctc
      841 cttggcgccg gagatatgct ctatataggc agtgagactt caaaaccgat aagaatccag
      901 tccggctaca taaccgaggg cgaaattaaa aagctcgtaa agttcctaaa agaaagacac
      961 caggacgacg tccctgaaga aattaatatc tccgaagtaa aagagggcga tacgcttgct
     1021 tcagcagatt ttggaagcga gggcgttgac ggagaagacg aggcgatatt caatgaagcc
     1081 aaaaaagttg ttatgtccac gcaaaaagcg tctacaacac tcctacaaag aaaacttaaa
     1141 ctagggtatg ccagagcatc tagaataatg gacttgctgg aagaaagagg cgttatcggt
     1201 cccggagacg gagcaaagcc tcgcgaggtc tatatgaaat cgggcgataa tatgggcggg
     1261 ggcggagctg tatcggccct gattaatgaa tacgaaggcg cggaaatagc ggaggaagag
     1321 gagaaagaag gaactgacct agaagcaacg atggcgcgag cagaaagcac tgaagaagaa
     1381 gcggaaggta aagaagagcc gacagaagaa gaggaagaat ttgtataaaa aagatttagg
     1441 gtaaataatg agttcatgcg agataggata aggaccaata taaaaatatc ggttatcggc
     1501 ttgataactt tagcgatatt ttcctatgcc tattttgaaa ctatcgaggt cttaagaggc
     1561 cccataatag aaataaagag ccccgagagc ggctacaccg caaccaacaa agtggtagaa
     1621 atatccggca acgtaaagaa cgtgagctat atgtatctaa atggaaaacc aatctttgcc
     1681 gacacttcgg gtaaatttaa ggaaaaggtt atcttaaacg acggttataa ttcggttttt
     1741 atagaagcaa aagataggat tggaaacaaa aaatcagaaa tattagaact tatcgtaaat
     1801 aataacaagg caagcatcgg agatggcctg gccagggacg aagacgctct caagagtatc
     1861 taataacaca taagttgaga caggtgaact tcccctgctt tttccggcgc atataatatt
     1921 attgatatta ataattttat gaattcaccc tgtttagtaa tccaattgag taaaatttta
     1981 ttaaaaaata aaatttaaat tagcggagct aattttactc aaccggataa aatttgtttt
     2041 ctaactaaaa cgagaactaa acgggattgc agtcgaatca ttaacaacat aaatataagt
     2101 aaaaacaatt aaacaaattt ttacttaacg acccagggtt caccctgtta agtaaaattt
     2161 tctttagaaa atttaaaatt tgtttatagc ggggtaataa ccaaaaggaa gaccgtcgaa
     2221 atattagaaa aataagatta taggtaaaaa caattaaaca aatttttact taacagggta
     2281 aaatggcaaa aaaatcagct gagaaaacgg aaaaagccgg caaaagcctt ggggcaaatg
     2341 tggataacgc tattaaggca atccaaagca agttcggaga aggagcaatt atgaaacttg
     2401 gcgaaaggcc gaaagttggc gtagatgcca tccctacggg ctcaatagga atagatatag
     2461 cgttaggggt cggagggctt cctagaggca gaataatcga aatattcggc ccggaatcgt
     2521 caggaaagac taccctcgcc cttcatgtcg tgtctgaagc gcaaaaaaag ggaggagtgt
     2581 gcgcttatat agacgccgag cacgccatgg atccggaata cacgaaaaaa ttgggcgtaa
     2641 aaatagagga ccttctcata tcccagccgg acaacggaga acaggctctt gagatcgctg
     2701 aaagccttat caggaccgga gggattgatg taatagtcat tgactctgtc gccgcactca
     2761 caccaaaaga tgagatcgaa ggagatatgg gggcacacca cgtaggaaaa caagctaggc
     2821 ttatgtcaca ggccctcaga aagatgaccg cgctagtggc aaagagtaaa accgtcgtga
     2881 tctttattaa ccagattcga atgcagatag gagttatgtt tggtaatccg gaa
//
LOCUS       DAOTEX010000079         2763 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3118
            NODE_29103_length_2763_cov_3.895495, whole genome shotgun sequence.
ACCESSION   DAOTEX010000079 DAOTEX010000000
VERSION     DAOTEX010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868142
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2763)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2763)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 883
            CDSs (total)                      :: 833
            Genes (coding)                    :: 829
            CDSs (with protein)               :: 829
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2763
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29103_length_2763_cov_3.895495"
                     /isolate="AMR_MDS_3118"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..207
                     /locus_tag="P5056_04145"
     CDS             <1..207
                     /locus_tag="P5056_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62930.1"
                     /translation="ILAIGFMALAMSYVICQRRRMVRSNLLFGMTALGVVVIFLLITY
                     GAAKNSREVINVLDMLVDDAKNGR"
     gene            353..613
                     /locus_tag="P5056_04150"
     CDS             353..613
                     /locus_tag="P5056_04150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HRY62931.1"
                     /translation="MAFHYIYVLKSLKDRRNYVGYTKNLKFRFEQHQKGLVESTKKRR
                     PFELIYSEACLDQKDATKREKYLKTIYGKRFIKSRLKSYLIG"
     gene            614..2158
                     /locus_tag="P5056_04155"
     CDS             614..2158
                     /locus_tag="P5056_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HRY62932.1"
                     /translation="MISRIHSAQVVGLKPHVIDIEVDISKGMNSFSIVGLGDKAIEES
                     KDRITAAIKNSNLAPSGKGSKKIIVSLAPADLKKEGPAFDLGIALGYLRSSEVLDFDP
                     KGKIFLGELSLDGKIRGIKGALIITEKAKRDGFEEIYLPEENAEEAALVEGIKIYPCK
                     NLLQVFKHLNKAEEERKSIAHAPKTELKNKKETTFNIDFGDVKGQESAKRGLEIAAAG
                     GHNITLFGPPGTGKTMLAKAFSGILPTLRPEEIIEVTGIHSASGTLEDDIITTPPFRS
                     PHHTSSYISLVGGGAYPKPGEITLAHRGVLFLDEFPEFEKRVLEALRQPLEDRVVSIS
                     RAKGSIKFPASFILVAAMNMCPCGNKGLKEKECICNMSAINKYQRKISGPIMDRIDMW
                     VLVPQIDHNKLGEKSDDAVEKSEDIKKRVERAREIQTERFKDSDGKIKTNSEMGVRDM
                     KKYASLGDKCRDLLVSYATKLEMSARAHHRVIKLARTIADLDGAEQISEKHILEALQY
                     RPKDVF"
     gene            complement(2412..>2763)
                     /locus_tag="P5056_04160"
     CDS             complement(2412..>2763)
                     /locus_tag="P5056_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62933.1"
                     /translation="MCQLNNIETLQESQNVEKVLNQKAETLTVEFTRLLAEVYPQHEK
                     DLFEKMTPDRVLALVIKYPEIKANTTVMELVTKIDQMRSAVYDQQILREKLKKDIRFN
                     KRNPFLIQFFIPKE"
BASE COUNT          859 a          608 c          655 g          641 t
ORIGIN      
        1 atcctcgcga tcgggttcat ggccctcgcg atgtcctacg tgatctgcca gagaaggcgc
       61 atggttcggt ccaacctcct cttcggcatg accgccctcg gtgtcgtcgt catcttcctg
      121 ctcatcactt acggtgcggc caagaactcc cgcgaagtga tcaacgttct cgacatgctc
      181 gtcgatgatg ccaaaaacgg tcgttaatgt cctacatgtt gccccaactt cggttggggc
      241 tttttcttgc ctctccgcgt ttttctaaaa cgaattcaga ttttaacaat attgagaaaa
      301 cgaaacaccg agagtagagt taaccctatt aagtaaaatt ttaaattttt taatggcatt
      361 ccattatata tatgtactaa aatcgctaaa agatagaaga aattatgtgg gatacactaa
      421 aaatttaaaa tttagatttg agcagcatca gaaaggactt gtcgagtcca ctaagaagag
      481 gagaccattt gagcttatct actcagaggc atgcttagat caaaaagacg cgacgaaaag
      541 ggaaaaatat ttaaaaacca tatatggcaa gaggtttatt aaaagtaggc tcaaatctta
      601 cttaataggg taaatgatat caagaataca tagcgctcag gttgtagggc ttaaacctca
      661 cgttatagac atcgaggtgg atatttctaa agggatgaac tcgttttcga tcgttgggct
      721 aggcgacaag gcaatagaag aatcaaaaga cagaatcacc gcggcgataa aaaactcaaa
      781 ccttgcaccg tccggaaagg ggagcaagaa gataatcgta tccctggcgc cggctgacct
      841 gaagaaagaa ggaccggctt ttgacctcgg catagcgctc ggatatttgc gctcatcgga
      901 agtgcttgat tttgacccca agggaaaaat atttcttgga gaattgtcct tggacggaaa
      961 aataagagga ataaaaggag ctcttattat tacagaaaaa gcaaaaagag acggctttga
     1021 agagatatat cttcccgaag aaaatgccga ggaagccgcg cttgtagagg gaataaaaat
     1081 atatccgtgc aaaaatctgc tacaagtttt taaacatttg aataaggcgg aggaagaacg
     1141 aaaatcaatc gcacacgctc caaaaacaga gctaaagaac aaaaaagaaa caacgttcaa
     1201 tattgatttt ggcgacgtaa aagggcagga gagcgccaaa agagggctag agatcgccgc
     1261 cgccggagga cacaatataa ccctattcgg cccacccggc acaggaaaaa caatgctcgc
     1321 aaaagcattc tccggtattt tgcccaccct aaggccggaa gaaataatag aggttacagg
     1381 aatacactcc gcttcgggca cgctagaaga tgacataatc accactccgc cttttcgctc
     1441 gccgcaccat acttcttcat acatttctct tgtgggagga ggggcatacc cgaagccggg
     1501 agagataacc ctcgctcata ggggagtatt attcctggac gagttccccg aatttgaaaa
     1561 aagagttttg gaagcattaa gacagccgct cgaagacaga gtcgttagca tttccagggc
     1621 aaaaggctct ataaagttcc cggcaagctt catattggtc gcggcgatga acatgtgccc
     1681 atgcgggaac aagggactga aagaaaaaga atgcatctgc aacatgtcgg caataaataa
     1741 atatcagaga aaaatttccg gtcctataat ggacagaatt gatatgtggg tattggttcc
     1801 tcaaatagac cacaacaaac tcggagaaaa gagcgatgac gccgtagaaa aatcagagga
     1861 tataaagaag cgagtagaaa gagccagaga aattcagacc gaacgcttca aagactcaga
     1921 cgggaaaatt aaaacgaaca gcgaaatggg cgtgcgtgat atgaaaaaat acgcctctct
     1981 aggagacaag tgccgggatc tccttgtatc ttatgcgaca aaattggaaa tgtccgccag
     2041 agcgcaccac agagttataa aacttgcgag aactatcgca gacctagacg gggcagaaca
     2101 aataagcgaa aagcatatac tcgaagcact tcaatacaga ccaaaggatg ttttctagca
     2161 aaaacacaga attctcctcc tgtcattccc gcgcaggcgg gaatccagtt tttaatttct
     2221 tcttgcttgc ctggattccc gtttccacgg gaatgacaat aaaataaatt ttataaaaaa
     2281 gcccggctac atttaagtag tcgggcataa cttcgcacga ggccttcttt attacgacta
     2341 gcgaggagcg agtgggcaag aactcttgca cacatcgtcc gagcgacgtc gtaaaaaagg
     2401 tttaatacct tttattcctt ggggatgaag aactggatga ggaacgggtt ccgcttgttg
     2461 aagcgaatgt ccttcttcag cttctcccgg aggatctgct ggtcatacac cgccgacctc
     2521 atctggtcga tcttcgtgac gagctccatc accgtggtgt tcgccttgat ctcaggatac
     2581 ttgatgacga gagccaggac ccgatcgggg gtcatcttct cgaagaggtc cttctcgtgc
     2641 tggggataga cctcggccag gagcctcgtg aactccaccg tgagcgtctc ggccttctgg
     2701 ttcaggacct tttcgacgtt ctgggactcc tgcagggtct cgatgttgtt gagctggcac
     2761 atg
//
LOCUS       DAOTEX010000080         2736 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3118
            NODE_29535_length_2736_cov_3.658709, whole genome shotgun sequence.
ACCESSION   DAOTEX010000080 DAOTEX010000000
VERSION     DAOTEX010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868142
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 883
            CDSs (total)                      :: 833
            Genes (coding)                    :: 829
            CDSs (with protein)               :: 829
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2736
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29535_length_2736_cov_3.658709"
                     /isolate="AMR_MDS_3118"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2094)
                     /locus_tag="P5056_04165"
     CDS             complement(<1..2094)
                     /locus_tag="P5056_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62934.1"
                     /translation="MSFFKNTIKIFLALLLANIFSGTAFAAIQVNKSQYYPDETVSFT
                     TSGGPWNVYDITTGYNFGGDDEYGSSPDVSDVYELSLDKVAGHEYAVIELTSRDVCTD
                     ASLSYEQCKETPEFVGEFKFRVISRPPAATTVTGGGGGPTAYRPEVSIYSPLDGKVYG
                     NNIEVIYEATDKNDAAGQSYLGLGNLPVTIYFSKTADTRQRITLGTNLPDKGVFKWNT
                     KDLPDGDLYNIIVSATDKVGETGETISGSFSLDHTSPVFTVKTDPTITRGEDVKIFVE
                     SSKELQIPPILKVTQNEFNSFDVAMTGDGFKFEGVYKVIGGYDGPAKISILGKDLAGN
                     ESDLIVSGGQFSVGILPPPKPIIISPLDRDIAPSGVVEVKGKARKDTEVILSLNGTDK
                     FSKKPDTNGEFTFLNLNLRPDFNFGVNVISIVSKDAAGNISESADLNLKYNVAPEIFI
                     EAPVKGQVLGANTQVTIKAADKNKDRLKFKYEVSNNNGSTWTDLNISPEKRSFVWDTT
                     TFEDGDYIMRVTVSDGTSGKVAITEGLIIKNLLPTFSFTDGDKTVTNKKEIVISGVVT
                     SSTKMPIRPNLLSVEYSLDGGGDWIPANILSGLNTSEIKFNISLNFSEEGTRHLLLRA
                     KDDREIYGRGIKTVIVIFEPPTIPSITSLKEGTIIDNSFDGDKLTAGVQIKIEGTSKP
                     NVFVRVVVDGVTFSGK"
     gene            complement(2091..2456)
                     /locus_tag="P5056_04170"
     CDS             complement(2091..2456)
                     /locus_tag="P5056_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62935.1"
                     /translation="MKGITTRKIKKIFRDNLPVIVILSALILLAFYYQYAWRNQKISG
                     EDVIYRVGKMINLPADEVPTIATVTNLEPLKDNVFFTNAKLGDTVLIYAKSAKAILFR
                     TSENRIIEIGSLNIGKNTK"
     gene            complement(2467..>2736)
                     /locus_tag="P5056_04175"
     CDS             complement(2467..>2736)
                     /locus_tag="P5056_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62936.1"
                     /translation="VDSAGNKALEMIRRVIVGDEAAPKEEPIIIPEETQTTAEALKPV
                     EPQPEILATRDSNPEISGPDISEPKILPPQDSAIETIVENSVVAE"
BASE COUNT          719 a          644 c          495 g          878 t
ORIGIN      
        1 tttaccggag aatgtcacac cgtctaccac aaccctaaca aaaacatttg gcttggaggt
       61 tccttcaatt ttaatctgga cacctgcggt taatttatcg ccgtcaaaag agttgtctat
      121 tatggtccct tcttttaatg aggttatgga cggaattgtc ggcggctcga agataacaat
      181 aaccgttttt atccctctac cgtatatttc acggtcgtct tttgcacgaa gcaataagtg
      241 tctggttcct tcctcggaaa aattcaaaga tatgttgaat tttatttctg aagtattaag
      301 accgcttaat atgttggccg gaatccaatc ccctccaccg tctaaactat attcaaccga
      361 caataaattc ggcctaatag gcatctttgt tgagcttgta acaacgcccg agataacaat
      421 ttccttctta tttgtaaccg tcttgtctcc gtctgtgaac gagaaagttg gtaaaagatt
      481 tttaattatc aacccttcag ttattgctac ttttcccgag gtgccgtccg aaaccgtcac
      541 tctcataata tagtccccgt cttcaaaagt tgttgtgtcc cacacaaaag atcttttttc
      601 cggagaaata ttcaaatccg tccaagtgct accattatta ttagaaacct cgtatttaaa
      661 ttttagtcta tccttatttt tgtcagcggc ttttattgta acctgtgtat ttgctcctaa
      721 aacctgcccc ttaaccgggg cctctataaa tatttcaggg gccacattgt atttcaaatt
      781 aaggtcggcg gattcactga tgtttccggc ggcgtccttg gacactatgc ttatgacgtt
      841 tacaccaaag ttaaaatccg gcctaaggtt aagatttaaa aaagtaaact cgccgtttgt
      901 atccggtttt ttactaaact tatctgtccc gttcaaagac aatattacct ctgtatcttt
      961 tctggctttt ccttttactt ccacaacacc ggaaggagct atatctctat ctaacggcga
     1021 tatgattatc ggttttggag gtggaagtat tccaacgctg aactgcccgc cgctaacaat
     1081 gaggtcgctc tcgttgccgg ccaggtcttt acctaaaata cttatcttgg caggtccgtc
     1141 gtatccgccg ataaccttat aaacgccttc aaatttaaac ccatctcctg tcattgccac
     1201 atcaaaggag ttaaattcat tttgggtcac cttcaaaata ggaggaattt gcaattcttt
     1261 cgacgactcc acaaaaatct taacgtcctc tcctcttgtt attgtgggat ctgttttgac
     1321 tgtaaatacc ggagaggtat gatccaggct gaaactaccg gaaatggttt ctcctgtttc
     1381 gcctacttta tcagtggcac taacaataat attgtataaa tcaccgtccg gcaggtcttt
     1441 tgtgttccat ttgaaaactc ccttatccgg caaattagtg cctaaggtaa tcctctgcct
     1501 cgtatccgct gtttttgaga aatatattgt taccggcaaa tttccaagac ccaaataact
     1561 ctggcccgcg gcatcatttt tatctgtggc ctcgtaaata acttcaatat tgtttccata
     1621 aaccttacca tcgagaggcg aataaatgct tacttccggc ctgtatgcag taggtcctcc
     1681 cccgccgccg gtcacagtcg tagcagccgg tggccgagaa ataactctaa atttgaactc
     1741 acccacaaat tccggagtct ctttacactg ctcatagctc aaagaggcgt cagtgcaaac
     1801 atctctgctt gttaattcta taaccgcata ttcatgaccg gctaccttat ctaaagacag
     1861 ctcataaaca tcggacacgt cgggactact gccgtattca tcatctcccc cgaagttgta
     1921 tccggttgtt atatcataaa cattccaagg tcctccgctt gttgtaaacg acacggtctc
     1981 gtccggataa tattgtgact tattaacctg aatagcggca aaagcagtcc ccgaaaatat
     2041 attcgcgagc aacagtgcta aaaatatttt tattgtattt ttaaaaaagc tcattttgtg
     2101 tttttgccga tattcaacga acctatctct ataattctat tttcacttgt tctaaataaa
     2161 atagccttgg ctgattttgc atatataagc acggtgtcgc ccagcttggc attggtaaaa
     2221 aaaacattat ctttaagcgg ctctaaattg gtaaccgtag cgatggtcgg aacttcatcg
     2281 gccggaagat ttatcatctt cccgactcta taaatgacat cttctccgga aattttttga
     2341 ttgcgccatg catattggta ataaaaagca agcaaaatta aggccgacaa aatgacaata
     2401 acgggcaaat tgtctctaaa tattttcttt atttttctgg tcgttattcc tttcattatt
     2461 caagagttat tcagctacaa cactattttc tacgattgtt tctatggcgc tatcctgagg
     2521 tggtaaaatt ttgggctccg aaatatccgg cccgctaatc tccggattgg agtctcgggt
     2581 agctaaaatc tccggttgcg gctccaccgg cttcagagcc tctgctgtgg tctgtgtttc
     2641 ttcgggaatt attattggtt cttctttagg cgccgcttca tccccgacga taaccctcct
     2701 tatcatttct agggccttat ttcccgcaga atctac
//
LOCUS       DAOTEX010000081         2604 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3118
            NODE_32025_length_2604_cov_5.012554, whole genome shotgun sequence.
ACCESSION   DAOTEX010000081 DAOTEX010000000
VERSION     DAOTEX010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868142
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2604)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2604)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 883
            CDSs (total)                      :: 833
            Genes (coding)                    :: 829
            CDSs (with protein)               :: 829
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2604
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32025_length_2604_cov_5.012554"
                     /isolate="AMR_MDS_3118"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            137..1033
                     /gene="secF"
                     /locus_tag="P5056_04180"
     CDS             137..1033
                     /gene="secF"
                     /locus_tag="P5056_04180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HRY62937.1"
                     /translation="MFIVKHKYFFVGLSLIMFLLSCFSIFHYGLNLGIDFKGGSIMEI
                     DYNDVRPDVTAVKEALSSLNIGETVVQETGDKGLLIKMRDLKEGERVSVVKALAINGA
                     KFDEKRFDSIGPVIGEEMSRKAVVAIVLVSIMIALFIAFAFRHVSQPVSSFKYGIAAL
                     IALLHDTVIPTGIMAYMGAKYGTEMDLLFVSAILAILGISVHDTIVVFDRTRENLKNK
                     VSKSFEETVGISLNQTFVRSINTSATTIFTLLALYFLGGESTKTFSLILAVGVFFGTY
                     SSVFLASPLLLLMEGWQGKRKK"
     gene            1257..1604
                     /locus_tag="P5056_04185"
     CDS             1257..1604
                     /locus_tag="P5056_04185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62938.1"
                     /translation="MGWTVYFENGETLRGFIKGALLNRPLPPGGKRKRSGGIVDTSKP
                     QRKISHISVGRLMGASSMFDRAYVDNIAEASITLKTDVRFGGVKEGKIFFVNAEDEEV
                     AFFPIIEIKEIVK"
     gene            1624..2070
                     /locus_tag="P5056_04190"
     CDS             1624..2070
                     /locus_tag="P5056_04190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62939.1"
                     /translation="MDGMIREPFASGEIVVQEHLKDVLRILEFMTWNDSGTGTFSASL
                     DKYPEYPVYLNAVDDKETYEFKKVADNLRWTSDPMLTAMELGAPMGFYRSVVGRYKPD
                     MTRLGLSLVSRVDRFQNYIVFYDSRDRKVGFIKAYFVKQFTLEMRS"
     gene            2314..>2604
                     /locus_tag="P5056_04195"
     rRNA            2314..>2604
                     /locus_tag="P5056_04195"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          719 a          465 c          645 g          775 t
ORIGIN      
        1 atttaaatta gcggagctaa ttttactcaa ccggataaaa ttactttcgg tgaatgaatc
       61 ggttgggggt taaaatagtc gatactttaa taaaataatt ttaaaaagta aaaagtaatt
      121 tttacttaat aggtaaatgt ttatcgtaaa acacaaatat ttcttcgtcg ggttgtcgct
      181 cataatgttt ctactttctt gtttttctat ctttcactac ggcttaaact tgggtataga
      241 tttcaaggga ggatcaatta tggagataga ttataatgat gttcgaccgg atgttactgc
      301 ggttaaagaa gccctctctt cgttaaatat aggagagaca gttgttcagg agaccggtga
      361 taaggggctt ctcataaaaa tgagagatct taaggaaggc gagagggtga gcgtagttaa
      421 ggcattagcc attaacggcg ccaagtttga cgaaaaaaga tttgattcca ttggtcctgt
      481 tatcggagag gagatgtcca gaaaggctgt ggtcgccatt gtccttgtct ctataatgat
      541 tgccttgttt atcgcttttg cgtttaggca tgtgtctcag ccggtaagct ccttcaaata
      601 tggtatcgct gcccttatcg ctcttcttca tgacacagtt attcctacgg gcattatggc
      661 ctatatgggg gccaaatatg ggacagagat ggacctgctt ttcgtctcgg caattctcgc
      721 catacttggt atttccgttc acgacacgat cgttgttttt gatagaacaa gagaaaactt
      781 gaaaaacaaa gtttcaaaga gctttgaaga aactgtgggg atttctttga accaaacatt
      841 cgtaagatcg atcaatactt ctgcgacaac catcttcact cttcttgcgc tgtatttctt
      901 gggaggagag tcaacaaaga cattctctct cattttggcg gttggagttt tcttcggaac
      961 ttactcttct gtattcttgg catcaccgtt acttctactt atggagggat ggcagggaaa
     1021 aagaaagaaa tagaagatag aaagtggcgg gtaaaaaata gtaatatttt taaagcggcc
     1081 ccggatgggc cgcttttggt ttctcttgct cttcggttgt cattcccgcg gaggcctgcc
     1141 tgcgtaggca ggcgggggaa tccatttgtt ggttgttttt attttcaaaa atgttagtct
     1201 tggaactggt ctattgatct ttaaaaaata ataagtaacg aaaggggaga tgagagatgg
     1261 gctggacagt ttactttgaa aatggagaga ctttaagggg tttcattaag ggagcgctct
     1321 tgaatagacc tcttcctccc ggtggaaaga ggaagcgttc cggggggatt gtcgacacaa
     1381 gtaagcctca acggaaaata agccatattt ccgtgggacg tcttatgggc gcttcttcaa
     1441 tgtttgaccg cgcttatgtg gacaacattg ctgaggcctc aattacttta aagacggatg
     1501 ttcgttttgg aggtgttaaa gaaggaaaga tattttttgt taatgcggaa gacgaagaag
     1561 tagcgttttt ccccatcatt gaaattaaag aaatagtgaa ataaccgaaa ggggagatga
     1621 gagatggatg gaatgatcag ggaacctttc gcttcagggg agatcgtggt ccaggagcac
     1681 ttgaaagatg tcttaagaat tttagagttc atgacgtgga atgattcagg aacaggaacc
     1741 ttttctgcat ccctcgataa atatcccgaa tatcccgtgt acctcaatgc ggttgacgac
     1801 aaagagacgt atgagtttaa gaaggttgca gataatctta gatggactag tgacccgatg
     1861 ctgacagcga tggaattggg cgccccgatg ggtttttaca ggtctgtcgt tggaagatac
     1921 aagcccgata tgaccaggct tggtctcagc cttgtctctc gtgtcgacag gtttcagaat
     1981 tacattgttt tctatgattc ccgcgacaga aaagtcggtt ttatcaaggc ttacttcgtg
     2041 aagcagttca ccctcgagat gagatcatag tgcagtttat acggctcaac agagccgtat
     2101 ttttatttta tccgaatttt gtgagaaagt gacccaaaat acttgacaat tgttttggct
     2161 tcgtatagat tatacagacg aaagaaacga acggcctttg aaaattattg agcgacacat
     2221 ttagttatgt gatctaattg tttgtttagg aacagataag aatctagtat tctctatttt
     2281 gttccgttct atttatagaa atttttaaat ttcttttttc agagtttgat cctagctcag
     2341 gatgaacgct ggcggcgtgg ataaggcatg caagtcaagg ggcccgcaag ggcaccggcg
     2401 aacgaggtag taacacgtgg gtacgtacct tcaagtctgg cacagctcgt cgaaagacgg
     2461 ggtaattccg gatagtccct tcggggtaaa gtcgtaagac gcttgaagaa cggcctgcgc
     2521 agtatcagct agttggtagg gtaatggcct accaaggcta tgacgcttag gggacctgag
     2581 agggtgaccc ccaccgatgg tact
//
LOCUS       DAOTEX010000082         2596 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3118
            NODE_32192_length_2596_cov_4.202676, whole genome shotgun sequence.
ACCESSION   DAOTEX010000082 DAOTEX010000000
VERSION     DAOTEX010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868142
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 883
            CDSs (total)                      :: 833
            Genes (coding)                    :: 829
            CDSs (with protein)               :: 829
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2596
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_32192_length_2596_cov_4.202676"
                     /isolate="AMR_MDS_3118"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="P5056_04200"
     CDS             <1..210
                     /locus_tag="P5056_04200"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HRY62940.1"
                     /translation="MEGESKNMIQNENFDGPTVFIMGNESKGLEKELISLCDKLIAIP
                     MDPKCESLNVAVSAGIALFLKSNKI"
     gene            324..1226
                     /locus_tag="P5056_04205"
     CDS             324..1226
                     /locus_tag="P5056_04205"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HRY62941.1"
                     /translation="MENKENFLQKILELSPSLIYVCALSEGKYKTIYLNKTIREVVGF
                     TAREKDGEEAPQLSSIINPDDIDTLLAGHKHLNTAPEGKQMEVEFRVRAQDGGWRWLK
                     SNESVFARDASGNIKEILGVAQDITEQKTIQAKLKSSEKQFQTLIEKGNDGVLIVQGD
                     GIVKYVNPILCQMSGYPLQEFVGKPFVSFLPQEYAKIVMERYRKRLSGQDVPNRYEIA
                     FVRKDGSILDVELNSSLVEYDGVPAVMAIVRDIADQKKQKAELEKHLETINKLNQLLL
                     GREVRMAELKNEIEELKRKLPNPA"
     gene            1241..1972
                     /locus_tag="P5056_04210"
     CDS             1241..1972
                     /locus_tag="P5056_04210"
                     /inference="COORDINATES: protein motif:HMM:NF013660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62942.1"
                     /translation="MILIKKKRGETPLQALCRIRKNKPGLASEKLSYIGRLDPMAEGE
                     MILLVGEKENKERKKYLNFDKEYEAVFLAGVSTDSGDILGFLNKTQNKELPKEKIKCA
                     VENLKKIKSQKYPWMSGKTISGKKMFDIYKEGDAEDIKRPSIKVKIHKISNLRISRLS
                     KKKLESDILKIIPKVAGDFRQKETIKKWNDFLAETNSKYFQTFSFKIKVSSGTFIRGF
                     CEELEKYLDTPTLLYRLKRTKIFYK"
     gene            2015..>2596
                     /locus_tag="P5056_04215"
     CDS             2015..>2596
                     /locus_tag="P5056_04215"
                     /inference="COORDINATES: protein motif:HMM:NF014656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HRY62943.1"
                     /translation="MTNRLYWFRFTLLAIFVVALVFVSTYYLGGFGAAGDSTETERFV
                     VSKGMSNLDVVKKLKTDDFIISEWAFNFAFNWEKFGWQIKPGAYKISKSMTAWQMAGV
                     FTNEPYMKWVTIPEGLRKEEVAVILKNELGWSDEKASNWIAKDTAASPDYTEGVYFPE
                     TYLIPVEESGADVAKRLRVKFEEKYSEFYTQALG"
BASE COUNT          917 a          427 c          584 g          668 t
ORIGIN      
        1 atggaagggg aatcaaaaaa tatgatacaa aacgagaatt ttgacggacc aaccgttttt
       61 attatgggca acgagtcaaa ggggctcgaa aaagaattga tttccctttg tgacaagctt
      121 atcgcgatac cgatggatcc aaaatgcgag tctctgaatg tagcggtatc cgcggggata
      181 gcgctattct taaaaagtaa taaaatatag gcatggatcc tgtggtgaaa ttttactaaa
      241 agatacgatg gttttataaa aatagttttc tataaataat attagtcgag caattaataa
      301 aatagttaaa attctactac gggatggaaa acaaagaaaa ttttttacaa aagattttgg
      361 agctaagccc gagcctaatc tatgtatgcg ccttatctga aggaaaatat aaaactattt
      421 atttaaataa aacaatcaga gaagttgtcg ggtttaccgc ccgggaaaaa gacggggagg
      481 aagcaccgca actttcttca atcataaatc cggacgacat agacacactt ttagccggcc
      541 acaaacatct taataccgcc cctgaaggta aacagatgga agtggagttc agagttcgtg
      601 cacaagacgg cgggtggagg tggcttaaaa gtaacgaatc ggtcttcgca agggatgcat
      661 cgggtaatat aaaggagatt ttgggggtcg ctcaagatat taccgagcaa aaaacgattc
      721 aagcaaaatt aaaatcttcc gaaaaacaat ttcaaacctt aatagaaaaa gggaatgacg
      781 gcgtcttaat cgtgcaggga gatgggatag ttaaatatgt aaatcctatt ctgtgtcaga
      841 tgagcggtta tcctttgcaa gagtttgttg gaaaaccttt tgtgtctttc ctcccgcagg
      901 aatatgcaaa aatagttatg gaaagataca ggaaaaggtt gagcggacaa gacgtgccca
      961 acaggtatga gatcgccttt gtaagaaaag acgggagcat tcttgatgtt gagctaaact
     1021 cttctttggt tgagtatgac ggtgttcccg cggtaatggc aatcgttaga gatatcgcag
     1081 atcaaaaaaa acaaaaagct gagctggaaa aacacctaga aacaataaat aaattaaatc
     1141 agttattgtt gggcagggag gtcagaatgg cggaattgaa aaacgagata gaagaactta
     1201 aaagaaagct tcctaatccc gcttaattta tttaataaaa atgatactta taaaaaagaa
     1261 gcggggagaa actcctcttc aggcgctttg ccgtatcaga aaaaataaac ccggattagc
     1321 ctcagaaaaa ctctcatata tagggcgcct tgatccgatg gcggaaggcg agatgatact
     1381 gcttgtcgga gaaaaagaaa ataaggagcg aaaaaaatat cttaattttg acaaagaata
     1441 tgaggccgtt tttcttgccg gagtttctac agattcaggc gatatcctgg ggtttttaaa
     1501 taaaacacaa aataaagagt tgcccaaaga aaaaataaaa tgcgctgtgg agaatttaaa
     1561 aaagataaaa agtcagaaat atccgtggat gtcgggaaag acgatttccg gcaaaaagat
     1621 gtttgatata tataaggagg gggacgccga agacattaaa aggccgtcta ttaaagtaaa
     1681 aatacacaaa atatctaact taaggatatc gcgcttgtct aagaaaaaat tagagagcga
     1741 tatcttgaaa attattccta aagtagcggg agatttccgg caaaaagaga ctattaaaaa
     1801 atggaatgat tttttggccg aaacaaattc taaatatttc cagacctttt cttttaagat
     1861 aaaagtctct agcgggacat ttataagggg tttttgtgaa gaacttgaaa aatatttaga
     1921 tactccgaca ctgctatata gactcaaaag aacaaaaata ttttataaat aattccgctt
     1981 ttaattaaac gtctccggtg ataagattaa acgaatgaca aaccgtttat attggtttag
     2041 gttcactctt ctggccatat ttgtagtggc tcttgttttt gtgtccactt attatttggg
     2101 cggatttggg gccgcggggg acagcaccga aacagagaga ttcgtcgtct ccaaaggtat
     2161 gtctaacttg gatgtagtca aaaaactaaa aaccgacgac ttcataatta gcgaatgggc
     2221 ttttaatttt gcattcaatt gggaaaaatt cggctggcag ataaaaccgg gggcatataa
     2281 aatttcaaaa tcaatgacgg cttggcaaat ggccggcgtt tttacaaacg aaccgtatat
     2341 gaaatgggtg acgataccgg aagggctccg aaaagaagag gtcgccgtta ttcttaaaaa
     2401 tgagctggga tggagcgatg aaaaagcttc taactggatt gcaaaagaca cggccgcttc
     2461 tcccgactat acagaaggag tctatttccc cgaaacatac ctcattcccg tcgaagagag
     2521 cggggcggat gtcgccaagc gcttgcgtgt aaaatttgaa gagaaatatt ctgaatttta
     2581 cacgcaagcg cttggc
//
LOCUS       DAOTEX010000083         2510 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3118
            NODE_33973_length_2510_cov_3.484318, whole genome shotgun sequence.
ACCESSION   DAOTEX010000083 DAOTEX010000000
VERSION     DAOTEX010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868142
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2510)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2510)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 883
            CDSs (total)                      :: 833
            Genes (coding)                    :: 829
            CDSs (with protein)               :: 829
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2510
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33973_length_2510_cov_3.484318"
                     /isolate="AMR_MDS_3118"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            123..749
                     /locus_tag="P5056_04220"
     CDS             123..749
                     /locus_tag="P5056_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62944.1"
                     /translation="MNYTKEQLNNIFENLPSDIQNAIYSVESADAVESIGKKYSLHMD
                     QIAALGRETGRVLLGITDPMDFVNQINEKLGVGKTVSSQLVFEINEKIFVPIKDSLRQ
                     IHSQAPMTKPAPVPAPNVVKNISTMELNVPKKTMDVLQGNIQATPRGDKTEILGFSAE
                     KKEMQAKTEGQLNIMEEKMKGGFNIPKAETEISKREPSRVVDPYREPI"
     gene            910..1329
                     /locus_tag="P5056_04225"
     CDS             910..1329
                     /locus_tag="P5056_04225"
                     /inference="COORDINATES: protein motif:HMM:NF024078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HRY62945.1"
                     /translation="MQFQVPQFIEIEDKVVGPFTFKQFIFVLGAAGGGFFIYRTVWAP
                     LSYILIALVSGFSLALAFYKINDRPFINVVESWFKFSLGSKRYLWKKEATVTQTSAGE
                     IENRITGALESKPIVPRLTAGKLKDIAWSLDIKEKIK"
     gene            1545..2195
                     /locus_tag="P5056_04230"
     CDS             1545..2195
                     /locus_tag="P5056_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62946.1"
                     /translation="MAEAKATQDFVQVKEIRDGIVVLKDGTMLMIFMCSSLNFALKSA
                     DEQQAIILQYQNFLNSLDFTFQIVVQSRRLDIRPYLASLSERMGSQPNELLKIQLKEY
                     INFIRSFTEGVSIMEKSFYAVVPYQPPIFQTSSQSGVASKVFGIFGGKKDTREQKEQE
                     LLTFEENRTQLEQRSSVVTQGLMRCGVRSVALGTEELIELFYKIFNPEESGGVPKF"
BASE COUNT          878 a          444 c          574 g          614 t
ORIGIN      
        1 cttaaaaaag cgggaagaag agagattgcc aatgtcttga acgaaaaact tgaaaagaaa
       61 gttaagagta aaacaggaaa cttggcgcaa gacctatttg aagaaggcgg aggaatataa
      121 aaatgaatta cacaaaagaa caactaaata atatttttga aaatcttcct tctgatattc
      181 agaacgctat atattccgtc gaatctgccg acgcggtaga atcaatcgga aaaaaataca
      241 gcctgcatat ggatcaaatt gcggcacttg gaagagagac gggcagggtg cttctcggga
      301 taacagatcc gatggatttt gttaatcaaa taaatgagaa attaggggta gggaaaaccg
      361 tatccagtca attagtgttt gagataaacg aaaaaatatt cgttcctata aaagattctt
      421 tgagacaaat acactcgcaa gcccccatga cgaagccggc tccggtgccg gccccgaatg
      481 tagtcaaaaa tatttccacg atggaactta atgtccctaa aaaaacgatg gacgttctgc
      541 agggcaacat acaagccaca ccaagagggg ataagacgga aattctaggt ttcagcgcag
      601 aaaaaaagga aatgcaagcc aaaacagagg gtcagctcaa tattatggag gagaaaatga
      661 agggcggatt caatattcca aaagccgaaa cagaaattag caaaagggag ccgagcagag
      721 tagtcgatcc ttacagggag ccgatataat taattagtaa ttaataatta cgaattacag
      781 atggtcgtca ttgtgaacgg agcgaagcaa tccagattta cgaggtgata tccctagatt
      841 gccgcgctcc gctcgcaatg acacaacaat aataatttgt aatttttaat tcataattcg
      901 tcattaaata tgcagtttca agtcccacaa tttattgaga ttgaagataa ggtcgtcggg
      961 ccgtttactt tcaagcagtt cattttcgtg ctcggagcgg cgggaggagg gttcttcata
     1021 tacagaacag tttgggctcc tctgtcatat atacttatcg cccttgtttc cggtttcagc
     1081 ctcgctctgg ctttctataa aattaacgac aggcctttca taaatgttgt cgagtcatgg
     1141 tttaagttta gtcttggaag caagagatat ttatggaaaa aagaggcgac agtgacacaa
     1201 actagtgcgg gagaaataga aaacaggata accggtgcgc tggaatcaaa accgatagtg
     1261 ccgagactga ccgccgggaa gttaaaagat atcgcgtgga gtttggatat taaggaaaag
     1321 ataaagtaag aagtaggaag taagaattaa gaattaagcc aacaatagca gtcattgcga
     1381 acgaagtgaa gcaatccaga gtgtagtgta taaagcgaat tgccgcgctt cgctcgcaat
     1441 gacagggatg aagagtagta ggcgaagcag tgaggtgtta aaataagaat agagttttat
     1501 aaaaatattc ttaattcata attctttatt cataattcat atttatggct gaagctaaag
     1561 caacacaaga ttttgtacaa gttaaagaaa tacgagacgg catcgtggtt cttaaagacg
     1621 gcacgatgct catgatattt atgtgctcat ctctaaattt cgccctcaaa tcagccgatg
     1681 aacagcaggc gattatttta caataccaaa acttcctaaa ttccctagat ttcactttcc
     1741 aaatagttgt gcagtcaaga cgtttggata taagacctta tttagcatca ttgtcagaga
     1801 gaatgggttc tcagccaaac gaacttttaa aaattcagct caaagaatac ataaatttca
     1861 tcagaagctt cacagaaggg gtcagtatta tggaaaaaag cttctatgcc gtcgttccat
     1921 atcagccgcc gatcttccaa acatctagcc aaagcggtgt tgcttcaaaa gtttttggta
     1981 tttttggagg caaaaaagac accagggaac aaaaggaaca ggaactcctg acatttgaag
     2041 agaacagaac acagcttgag cagagatcca gcgttgtaac acaaggatta atgagatgcg
     2101 gagtcagatc ggtagccctt ggcacagagg agctaataga acttttctat aaaatattta
     2161 atccggagga gtcgggagga gtgccgaagt tctaactttt tgaaaaagtt agaactggaa
     2221 ttaagaatta ggaattaagg aaaagagaaa gaagactgtc atccccgtgg ggcttgtcct
     2281 cgacccgatc ggggaacggg gatccagtga agattaaaag atggaatccc gccttcgcgg
     2341 gagtgacaaa agagagttaa ttgatttaaa atggcattat tcggaaaaca agaagaaaac
     2401 aaaatagaag cgatacttcc gcaggatatt tatcaaagcg gaacgcttga actgcgtgac
     2461 gttatctcac cgtccgctct tgagattgga tcaaacacga tgaagatagg
//
LOCUS       DAOTEX010000084         2509 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3118
            NODE_34005_length_2509_cov_2.651182, whole genome shotgun sequence.
ACCESSION   DAOTEX010000084 DAOTEX010000000
VERSION     DAOTEX010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868142
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2509)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2509)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 883
            CDSs (total)                      :: 833
            Genes (coding)                    :: 829
            CDSs (with protein)               :: 829
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2509
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34005_length_2509_cov_2.651182"
                     /isolate="AMR_MDS_3118"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..680)
                     /locus_tag="P5056_04235"
     CDS             complement(<1..680)
                     /locus_tag="P5056_04235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62947.1"
                     /translation="MPKDISQFGKGCLIKEPSIRDYRLETMLARATILPSSYSLKDKI
                     GKIKNQGKSYSCVGQAIAYYVQLLNFLETGKQVEMSARDVYSTIYQPEGGAYIVDGMK
                     KICNSGCVEEKDAPSYENGVAPSEAFMKNRADITSQESENGMTYLAKKYCTFEKKNVE
                     KLKIAIYEGTGAVVACDGNNTCWADGEIEVPEKVNWAHGILMVGWDDTKKVFEFVNSW
                     NGWGFDGFG"
     assembly_gap    1348..1357
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(1491..1721)
                     /locus_tag="P5056_04240"
     CDS             complement(1491..1721)
                     /locus_tag="P5056_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62948.1"
                     /translation="MEIIEAITEYWGIILTFAGIIGSFSVLKYQNSEQEKRIECLEDE
                     NKDIKTMQQSIDTRLAQIQTDLEWIKRSIGKN"
     gene            complement(1723..1965)
                     /locus_tag="P5056_04245"
     CDS             complement(1723..1965)
                     /locus_tag="P5056_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62949.1"
                     /translation="MEKRQIIIACVIAFVISLGFNIQYGWKYLTNYVYQQGYQQGGEY
                     YVGAIIKTIEKEGSINIDGKTYIEKVAKKSATSTPE"
     gene            complement(1967..2293)
                     /locus_tag="P5056_04250"
     CDS             complement(1967..2293)
                     /locus_tag="P5056_04250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62950.1"
                     /translation="MNTTQKVIAGVAIASAIAGGAVLLPKTDAVTEGMTKKSATEVVI
                     TDSKKVSKTYTLDELEGNVKILDNLSTTLQESCSKRITDILSRLDKAKELVGQAKKLG
                     VKNSEE"
     gene            complement(2306..>2509)
                     /locus_tag="P5056_04255"
     CDS             complement(2306..>2509)
                     /locus_tag="P5056_04255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62951.1"
                     /translation="DGTACLFLMVNQSVELVSACSTYSNTKDNASTINVYWDSVNSDF
                     AIQNKIANNKNVKWIVGSTAFIQ"
BASE COUNT          624 a          571 c          403 g          901 t
ORIGIN      
        1 ccaaacccat caaatcccca cccgttccaa gaattaacaa attcaaatac tttttttgta
       61 tcatcccagc ccaccattaa aatcccgtga gcccagttta ctttttctgg tacttctatc
      121 tctccgtctg cccagcaagt gttatttcca tcacaagcca ccactgcccc agtaccctca
      181 taaatagcaa tctttaactt ttcaacattt ttcttttcaa aggtgcagta ttttttcgct
      241 aaataagtca ttccattttc tgattcttgt gaggtaatgt ctgccctgtt tttcataaat
      301 gcctcgctcg gtgctactcc gttctcataa cttggggcgt ctttttcttc cacacaccca
      361 gagttgcaaa tctttttcat tccatccaca atatatgctc caccctctgg ctgataaatg
      421 gtagaataaa catctcttgc agacatttca acctgtttgc ctgtttccaa aaagttaaga
      481 agttgcacat aatatgcaat ggcttgtccc acacaagaat atgacttacc ctgatttttg
      541 atttttccaa ttttatcttt taaggaatat gaagatggca agatcgtggc tctggcgagc
      601 attgtttcaa gtctataatc tcttattgag ggttctttaa taagacaccc tttaccgaat
      661 tgtgatatat cttttggcat aatttagtgg ggagtttatt ggcactcccc cagccttttt
      721 actttgccgt aaagtatagg ataactttga gtgtcatccc tcgttcatcg gcttcctctg
      781 ccagtttgtc tatttgccgt ttggcataat agatacgctc tggacaatcg cataggaaag
      841 ttcccatacg atgaggattg aggtctacct catttccaga cgtgttgacg ggtgcttcca
      901 ttgcatctcc ccttgttaat gccacgaagg tcgggcgtta agacgaggtc tgggcaacct
      961 ctcttaaagc aatagtcgca cgccttacga ggggctacca cgaggttacg cttacgacag
     1021 atagcacgat catctcacgc ctccttttgt taaagaactc caaaatggga aagtgtttta
     1081 ttagtctttt gtgttgggtt tccccgctac accagccgac actggcaaga ctaccctcca
     1141 ttggtttcag ttgcaaagtt attgcacgtg cttgaccgag acaggacttt cgtcctccaa
     1201 tgtttttagg actttcaacc tgcgttatta tcagggtaag ttcggatttt ctacttaccc
     1261 atattatcat tgttgcgata ccctcaactt tcccatattg gaatcctcta acattatttt
     1321 aataaaccat agtgggctta cgctcgcnnn nnnnnnnttc atacatatat aagattatca
     1381 attttactat atgtatcaga actgggggcg aaataccatc atcacttgtc ccaaatgatt
     1441 tagatatttt gcctttgcgc aggcggagat attccgccct cagtcctaat ttaatttttt
     1501 cctattgacc ttttaatcca ctctaaatct gtctgtattt gtgccaatct ggtatcaatg
     1561 ctctgttgca ttgtttttat gtctttgttt tcatcttcaa gacactcaat tcttttttct
     1621 tgttctgaat tttggtattt taatactgaa aaagaaccaa ttatacccgc aaaggttaaa
     1681 attattcccc agtattctgt tatcgcttca atgatttcca ttttattctg gcgtacttgt
     1741 tgctgatttt tttgcgacct tttctatgta tgtcttcccg tctatattga tgctcccctc
     1801 tttctctatt gttttaatga ttgcacctac atagtattcc ccaccttgct gataaccttg
     1861 ctgatataca taattggtga gatatttcca accatattgg atattaaaac ccaatgaaat
     1921 tacgaacgct atgacacacg ctataataat ttgtcttttt tccattttat tcttcgctgt
     1981 tttttactcc taatttcttg gcttgaccga ccaattcttt tgctttatca agccgtgata
     2041 aaatatctgt tattctttta gaacaactct cttgcaacgt cgttgacaga ttatcaagaa
     2101 ttttaacatt cccttctaac tcatcaagtg tatatgtttt agaaactttt ttgctatcag
     2161 taattaccac ctctgtggct gactttttag tcatcccttc tgttacggca tctgtttttg
     2221 ggagcaatac ggcgccccca gcaattgcac tcgctattgc cacgcctgct ataacttttt
     2281 gtgttgtatt catacttttt taattttatt gaataaaggc tgtggatccg acaatccact
     2341 ttacgttttt attattcgca attttattct gtatagcaaa atcactattt acagagtccc
     2401 aatagacgtt gattgtgctg gcgttatctt tagtgtttga atatgtagaa caggcactca
     2461 ccaattccac agattggttt accattaaga ataaacaagc agttccgtc
//
LOCUS       DAOTEX010000085         2493 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3118
            NODE_34343_length_2493_cov_3.753076, whole genome shotgun sequence.
ACCESSION   DAOTEX010000085 DAOTEX010000000
VERSION     DAOTEX010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868142
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 883
            CDSs (total)                      :: 833
            Genes (coding)                    :: 829
            CDSs (with protein)               :: 829
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34343_length_2493_cov_3.753076"
                     /isolate="AMR_MDS_3118"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..797)
                     /locus_tag="P5056_04260"
     CDS             complement(<1..797)
                     /locus_tag="P5056_04260"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HRY62952.1"
                     /translation="MVDFKKEIEALVEITLKEGASDLHITAGRKPSIRVSGELIPLIS
                     QPILSEQDTKGLLLELLSEENKKKFLETKDIDFAYDSPFGVRFRGNAFFQKGHVGGAL
                     RVIPKKIRGVGELNLPSVLNEFAFKEQGFFLVVGPVGQGKSTTLAAMVELINRERAKH
                     ILTIEDPIEYIFESDKSIIDQREVRFDTESFQSALKSMFRQDINVAMIGEMRTAETIS
                     TAVTAAETGHLILSTLHTNNAAQTIDRIIDSFPGEQQDQIRVQLASSL"
     gene            complement(886..>2493)
                     /locus_tag="P5056_04265"
     CDS             complement(886..>2493)
                     /locus_tag="P5056_04265"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HRY62953.1"
                     /translation="GLIFFKIKMAKDSNNLIPKRDIGGRLSNAEVLKYVPVESATYYK
                     FAPIDFKDGVLEFGITDPDSIEARDAISFTANKYNLPYKIFQVSESDFRVILDAYKEM
                     AGEVHEALQEFATTQGRPGAADIVTSELMEDIAAKKTEVTKEDAPMAKIVSVIIQNAV
                     EGNASDIHIEPGLDKVKVRFRMDGVLHTSLMLPMNIADAIVARIKILTNMKLDEKRIP
                     QDGRFSANIAGRQIDFRVSTFPTYFGEKVVMRILDFEKGVKKIESLGFSAEQLQAIKE
                     ALNAPYGLILLTGPTGSGKTTTLYSMLNEVDREENNVVSLEDPVEYNIQGVAQSQVRP
                     EIDYTFASGLRSILRQDPDIIMVGEIRDAETARLAIQAALTGHLVFSTLHTNTAVAAI
                     PRLMDMGIEPYLIAPTLILVIAQRLVGSLCEESKGEIPVTPDMKEDLTKQFADLPENI
                     RKQIKIPNNVYTAKPSATCATGTRGRTPVLEVLQVDREMQNLIFKNPSEEEVRNLARS
                     KGMLTMKEDAMLKAFDGEIGFEEVDKL"
BASE COUNT          607 a          616 c          493 g          777 t
ORIGIN      
        1 agggatgaag caagctggac tctgatctga tcctgctgtt cgcccgggaa agaatcgatg
       61 attctgtcta ttgtctgagc cgcgttgttt gtatgaaggg ttgataggat gaggtgccct
      121 gtttcagccg cagtcacagc cgtgcttatt gtttcagctg ttctcatctc ccctatcatt
      181 gcgacgttta tatcctgtct gaacatagac ttgagggcgg actggaagct ctcggtatca
      241 aatctgactt ctcgctgatc tattatagat ttgtcgcttt caaaaatata ttctatcggg
      301 tcttcaatag taagaatatg ctttgctctt tctctattta taagctcaac catcgcggca
      361 agcgtcgttg atttaccttg accgacagga ccgacaacaa ggaagaaacc ctgctcctta
      421 aatgcaaatt cattcaaaac gctaggaaga tttaactctc caacgcctct tatcttcttt
      481 ggtattacac ggagcgctcc gcccacatga cctttctgga aaaaagcatt tcctcggaat
      541 ctgacgccga aaggagaatc gtatgcaaag tctatatctt ttgtttcaag gaattttttc
      601 ttattttctt cgctcaaaag ctccaagaga agaccttttg tatcttgttc gctcaatatt
      661 ggttgagata tgagagggat gagctctccg gaaactctga tggacggttt tctcccggcc
      721 gtaatatgca gatcagaagc cccctctttg agagtgatct ccaccagtgc ctcaatttct
      781 tttttaaaat caaccatata tttatgaata atgaataatg aattaagaat tatgaataaa
      841 acacaaactt ttgccttatt cctaattcct accccctaat tctttttaaa gcttgtctac
      901 ttcctcaaaa ccaatttctc cgtcaaacgc tttcaacatg gcatcctctt tcattgtaag
      961 cattcccttg cttctggcca aatttctgac ttcttcctct gaaggattct tgaaaataag
     1021 attttgcatc tctctgtcca cctggagcac ctccaaaacg ggagttcttc ctctcgtgcc
     1081 ggtagcgcat gtcgccgaag gtttcgcggt ataaacatta ttaggtatct tgatttgttt
     1141 tctaatattt tccggaaggt cagcgaattg ttttgtaaga tcctctttca tatcaggcgt
     1201 aacgggaatc tcccccttag attcttcgca caaacttcct acaagcctct gagctattac
     1261 caaaataagc gtcggtgcaa tcaaataagg ctctattccc atatccataa gtctaggtat
     1321 ggccgcaacg gcggtattcg tgtgaagggt agagaatacc aagtgtcccg taagagccgc
     1381 ttgtatggcg agtctggccg tctccgcatc tcttatttct cccaccatta tgatgtccgg
     1441 gtcttgtctc aaaatggagc gcaaaccgct ggcaaaggta taatcaattt caggtcttac
     1501 ctgagactgt gccactccct gaatgttgta ttctaccggg tcctccaagc tcacaacgtt
     1561 gttctcctct ctatctactt cgttcagcat ggaataaaga gtggtcgttt taccggaacc
     1621 tgtcggtcct gtaaggagta taagtccgta tggggcattg agcgcctctt taatggcctg
     1681 caattgctct gcggaaaatc cgagactctc tattttcttt accccctttt caaaatccaa
     1741 gattctcata accaccttct ctccgaagta tgtcggaaaa gtggacacac ggaagtctat
     1801 ctgtctgccc gcgatattgg ccgaaaacct tccgtcttga gggattctct tttcgtccaa
     1861 tttcatattc gtgagaattt ttattctggc gacaatggca tccgcaatgt tcatcggcaa
     1921 cattaagctg gtgtgaagca caccgtccat tcggaatctc accttcacct tgtctagtcc
     1981 cggctctata tggatatctg aggcgtttcc ttcaaccgca ttttgaatga taaccgagac
     2041 aatcttcgcc atcggagcgt cctcttttgt gacttcagtt ttcttggcgg cgatgtcctc
     2101 cataagctcg ctcgtaacga tgtctgccgc gccgggcctg ccctgggtcg tcgcaaactc
     2161 ttggagagct tcatgcacct cccccgccat ttccttgtaa gcatccagaa taaccctaaa
     2221 atcagattct gaaacctgga atatcttgta aggaagattg tatttattcg ccgtaaagct
     2281 gatggcatct ctcgcctcta tactgtccgg gtctgttatg ccaaattcca aaacgccgtc
     2341 cttaaaatcg attggtgcaa atttgtagta tgttgcggac tcaaccggaa catatttcaa
     2401 aacctcggcg ttgctgagcc ttcctccgat gtctctcttt ggtattaaat tgttcgagtc
     2461 cttggccatc tttatcttaa aaaatattaa tcc
//
LOCUS       DAOTEX010000086         2432 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3118
            NODE_35714_length_2432_cov_3.874632, whole genome shotgun sequence.
ACCESSION   DAOTEX010000086 DAOTEX010000000
VERSION     DAOTEX010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868142
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 883
            CDSs (total)                      :: 833
            Genes (coding)                    :: 829
            CDSs (with protein)               :: 829
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35714_length_2432_cov_3.874632"
                     /isolate="AMR_MDS_3118"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /locus_tag="P5056_04270"
     CDS             complement(<1..427)
                     /locus_tag="P5056_04270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62954.1"
                     /translation="MSSFFIFNNLNFAEQMFGALVFAMTAWLAFDAYMIRRDFLTASR
                     GIGFGLLVLSQIFAAFSFGSENYEYVGHVIRLLGLILVLWNLLLEKPVERPSINAVFV
                     LPSIATALFFFNFWDAVLFFTIAFLSYRQYKNEDKKTLLP"
     gene            complement(449..1027)
                     /locus_tag="P5056_04275"
     CDS             complement(449..1027)
                     /locus_tag="P5056_04275"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRY62955.1"
                     /translation="MTPDYEKIWRLALAVVGSLFAIFILLNLLGKVFIEEMSQNLFVE
                     QAGAIDNEVAAISEVSPYFELQNLNGKLIKITDYKGSPVVVVFWNSWNDIAVSQIKIL
                     DEMSDKYSKIFKILTINNQEKRESVANFIRRGSYNKMEVLLDVNGGIGDAYEARNMPA
                     FYFIDKNGLLMDKYYGFLDEKQIVEKMSKFSN"
     gene            complement(1032..>2432)
                     /locus_tag="P5056_04280"
     CDS             complement(1032..>2432)
                     /locus_tag="P5056_04280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62956.1"
                     /translation="VTFSGKTDSSGKFSVSGVTLRKHGQNIISAYAEDEAGNKSGTKS
                     VGLIYNNAPVLVFLNPRPNRGLNHKTSVEFSVSDPDGDAIVKTILSMKKPGETSFKSV
                     DVNSPDNKFELDVSSFPEGGGYEMKLESGDGVSASSKTIQFYVDNTRPTIFVEGVPSR
                     NFKKDFRFEAIGYAEDGLSGVEFVEYSLDEAHWFKAIITKGMLTKRASFRVRHPFTLD
                     DGVYEIKFRSVDAAGNYSSAWAEQFVVDTMPPRLGSYELSYNGISLYPDEEGFIVVSG
                     KNLKIRASFEAGTKNAHIIIGDIRKELTRGTDGLWEGEFTIDKFGSYEIRYGAKDDFG
                     NAIDNKRIGTIGVVKNGTINVADSRGNTTDEVVEGADIGVYIFSEDRQRFERWPGEYY
                     GIANPAKSESDGSYALFLPAGKYQIYVQKEGFVKLKTNEFSIGSGKFLNYDFSIKRRE
                     GVRGAIEDMLEKIMIF"
BASE COUNT          726 a          613 c          381 g          712 t
ORIGIN      
        1 atgggagaag agtctttttg tcttcgttct tatattgtct ataagataaa aaggctatcg
       61 taaagaacaa aacagcatcc caaaaattaa aaaagaaaag agcggttgct atggacggca
      121 aaacaaaaac agcattgata gacggcctct ctaccggctt ttctaaaagt aagttccaaa
      181 gtacaagtat gaggccgaga agcctaataa catgccccac atattcataa ttctcagacc
      241 cgaaactaaa agctgcaaat atttgggata aaacaagaag gccaaaacct atccccctgg
      301 aagcggttaa aaaatctcgc ctaatcatat aggcatcaaa ggcgagccac gccgtcatgg
      361 caaacaccaa cgcgccaaac atctgttcgg cgaaatttag attattaaag ataaagaatg
      421 aggacatatt ttttaattat accacaaact aattactaaa ttttgacatc ttttcaacaa
      481 tctgtttttc gtctaaaaaa ccataatact tatccattag aagcccgttt ttatctataa
      541 aatagaacgc cggcatattt cttgcctcat aagcatcacc gatgccgccg ttcacatcca
      601 gcaaaacctc catcttatta tacgaccccc ttcttataaa attagccacc gactctcttt
      661 tttcttgatt atttatagtt aaaatcttaa aaattttaga atatttgtcg gacatttcgt
      721 ctaaaatctt tatttggcta acggcgatat cgttccagct attccaaaaa accacaacta
      781 cagggctccc tttataatcg gttattttaa ttaactttcc gttcaaattc tgcagttcaa
      841 aatacgggct aacctcgctt atggccgcga cttcattatc tatcgcccct gcttgctcaa
      901 caaaaagatt ttgagacatt tcctctataa atacttttcc aagcaagttt aataaaataa
      961 aaatcgcgaa caaggaacca accaccgcaa gagccagcct ccaaattttt tcataatcag
     1021 gtgtcatatt tttagaagat cattattttt tctaacatat cctcaatcgc cccccttaca
     1081 ccctctcttc tcttaataga aaaatcataa ttcaagaact tgccgcttcc tatactaaat
     1141 tcattggtct tgagtttcac aaaaccttct ttttgaacat aaatttgata cttaccggca
     1201 ggcaagaaca aggcatagct accgtcgctt tcgctcttgg ccggatttgc tatcccataa
     1261 tactcaccgg gccatctctc aaacctttgt ctgtcctcac taaaaatata aacaccgata
     1321 tcggcaccct cgactacctc atctgtagta tttccgcggc tatcagctac attaatggtc
     1381 ccgtttttaa caacaccgat agtcccgatt ctcttattat cgatagcgtt accgaaatcg
     1441 tccttagccc catatcttat ttcatacgag ccgaatttat caatcgtgaa ctcaccctcc
     1501 cacaacccgt cagttccccg agttaattct tttcttatat caccgattat tatgtgggcg
     1561 ttttttgtgc cggcctcaaa agaagcgcgg atttttaaat tctttcccga caccacgata
     1621 aatccttcct cgtcgggata caaagaaatt ccgttataag aaagttcata gctacccagt
     1681 ctcggcggca ttgtatccac tacaaattgt tcagcccacg cggatgaata attacccgcc
     1741 gcgtctacag agcgaaactt tatttcataa acaccatcgt caagagtaaa cggatgtcta
     1801 actctgaagc ttgccctctt tgttagcatg ccttttgtga taatcgcctt gaaccagtgc
     1861 gcctcatcaa gactatattc cacgaactcc accccggaca atccgtcttc cgcatagccg
     1921 atagcttcaa atctaaaatc ttttttgaaa ttcctactag ggacgccttc aacaaatatt
     1981 gtcggccttg tgttgtctac ataaaactga attgtcttgg aggatgcgct aaccccgtcg
     2041 cccgattcca gtttcatctc atatcccccg ccctccggga acgaagacac atcaagctcg
     2101 aacttattat ccggagagtt cacatctaca gacttgaacg atgtctctcc cggttttttc
     2161 atagataaaa tagttttcac aatggcgtca ccgtcagggt cagacaccga aaactcaaca
     2221 cttgttttgt gatttaaccc cctattcggt cttgggttta aaaagaccaa aactggagca
     2281 ttgttatata ttagaccgac actctttgtg ccgctcttat tgccggcctc atcctctgcg
     2341 taggctgaaa taatgttttg accgtgcttt cttaaggtaa cccccgacac cgagaacttg
     2401 cccgaactat cagttttacc ggagaatgtc ac
//
LOCUS       DAOTEX010000087         2325 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3118
            NODE_38445_length_2325_cov_3.885022, whole genome shotgun sequence.
ACCESSION   DAOTEX010000087 DAOTEX010000000
VERSION     DAOTEX010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868142
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2325)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 883
            CDSs (total)                      :: 833
            Genes (coding)                    :: 829
            CDSs (with protein)               :: 829
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38445_length_2325_cov_3.885022"
                     /isolate="AMR_MDS_3118"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..605)
                     /locus_tag="P5056_04285"
     CDS             complement(<1..605)
                     /locus_tag="P5056_04285"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HRY62957.1"
                     /translation="MIKNPIGAHYSIKDGYLGAFKSAISDNADALQIFAKSPASAKFR
                     SVTKEEADEVKGFKNRKQIRSVVIHGSYLINLAEKKLDANSYQIKSLAEDMKNAEALG
                     GIGAIVHAGKSLGQDKTEAINNFVQNIHKLLDTAKDANAKIIIENTAGQGTEMGHDLK
                     ELGEIYKKIGDKERVKICLDTAHAFGAGYDIKNNAEKVVED"
     gene            complement(803..1144)
                     /locus_tag="P5056_04290"
     CDS             complement(803..1144)
                     /locus_tag="P5056_04290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62958.1"
                     /translation="MENNHAQRNPLPVLAPLVVILLAMLFVVPVPPDNFPVVMVPAGQ
                     RAVAMFPRGAIGVYLPAVILLTLEGGELTPYKDYYVLSRKRGTDEVPIRKTIDIDSLP
                     FEYNIFIFQEQ"
     gene            complement(1294..2289)
                     /locus_tag="P5056_04295"
     CDS             complement(1294..2289)
                     /locus_tag="P5056_04295"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HRY62959.1"
                     /translation="MQYRHFSIEEREKIQLMWWKRESIRTIANAIGRSPSSVSREMTR
                     NFPKENKVYTSRLAHERALIHRKHRGRTDRLKNAEIRAYVVSHLKERWSPEQISGRIK
                     KDIGEHISHEAIYQYIYAQVYRNGWGLLKPMHEDLRGYLRHCRKRRMKKGLRRSQRVF
                     KPHGPSIEDRPKLVKDKKRVGDFEGDMVMSVGNKPGVNTLLERKTGMFFITRVKEKTS
                     IATNSAISKRMSVIPGHLKHTITLDNGTENSDWRELERLTGLKTYFAHPYCSGERGAN
                     ENTNGLLRDYFPKKTDFSMITDEVLALVEYKLNTRPRKRLNWRTPLEAWSVALES"
BASE COUNT          554 a          517 c          497 g          757 t
ORIGIN      
        1 atatcttcaa cgactttttc agcgttattt ttaatatcat atccggcacc gaaagcgtga
       61 gccgtatcga ggcagatttt gactctttcc ttgtctccga tctttttata tatctcgcca
      121 agctctttga ggtcgtgtcc catttccgtg ccttgtcccg ccgtgttttc aatgattatc
      181 ttagcgtttg cgtcttttgc ggtatcaagt aatttgtgga tgttttgaac gaaattattt
      241 atagcctcgg tcttatcctg gccaagagat ttgcccgcat gaactatcgc cccgataccg
      301 cccagagctt cggcattttt catatcttcg gcgagcgatt ttatttgata tgaattagca
      361 tcgagcttct tttctgcgag gtttatgagg tatgatccgt gaatgacgac acttcgtatt
      421 tgctttctat ttttgaatcc cttaacttcg tccgcctctt cttttgtgac ggatctgaac
      481 ttggccgaag cgggggattt ggcgaatatc tgaagagcat ctgcgttatc tgaaatcgct
      541 gatttgaaag cacccagata gccgtctttt atcgaataat gtgccccaat cggatttttt
      601 atcatgatga aatgatagca ttttattttt tagccgtcat cgcgaggaac gaagcaatcc
      661 agaaggtagt agctataatc tcgtaaagtt ttttaaagga aataaaaagc gccgactcgg
      721 atgagtcggc gtattcgatg gtttgctaga attttcttgg ttcagaaatt tttacagtgc
      781 agagccccta gaatgcgccc agctactgtt cttggaatat aaaaatatta tattcgaatg
      841 gcagactgtc gatatcaata gtttttctta tgggaacctc atccgtccct cgtttcctgc
      901 ttagcacata atagtctttg taaggcgtta attctccccc ctctagagtt aaaaggataa
      961 cagctgggag atacactcct attgctcctc gagggaacat cgcgaccgcc ctttgtccgg
     1021 cggggaccat gacaacggga aaattgtcgg gcgggactgg gaccacaaaa agcatagcaa
     1081 gaagaatgac cactaaagga gcaagcacag ggagtgggtt ccgctgggca tggttgttct
     1141 ccatctcagt cacacctttc gtgaggttct gttggggcca ttaggccgtg gattgtttaa
     1201 tttctgaatt taattgtcaa agaatgaact atctacctta tactttaagt gtagcacaaa
     1261 taggtataaa agtcaaggtt tatgcgggtg aattcaactc tcaagtgcaa cactccaagc
     1321 ttctaatggc gtcctccaat taagcctctt tctaggcctt gtattgagtt tgtactcgac
     1381 taaggcgagc acttcatcag ttatcatact aaagtcggtc ttctttggga aatagtccct
     1441 aaggagtcca ttagtgttct cgttagcccc tctttcccct gaacagtatg ggtgggcaaa
     1501 gtatgtctta agtccggtca gtctctctag ttctctccag tcgctattct ctgttccgtt
     1561 atcaagagtg atggtgtgtt taagatgacc tgggataact gacatgcgtt ttgaaatagc
     1621 agagttggtg gctatgcttg ttttttcttt gactcgagtt atgaagaaca ttccggtctt
     1681 tctctcgagc aatgtattaa caccgggctt gtttcccaca gacatcacca tatcgccctc
     1741 aaaatcaccc acacgtttct tgtctttaac taacttcggt ctgtcttcaa ttgacggtcc
     1801 atgaggctta aatactcgct gtgagcgcct taagcccttc ttcattctac gcttcctgca
     1861 gtgtcttaag tatccccgca aatcttcatg catcggctta agaagccccc aaccatttcg
     1921 atagacttgc gcgtagatgt attgatagat agcctcgtgt gaaatatgct ctccgatatc
     1981 tttcttaatt cttcctgaga tttgttcggg ggaccacctt tctttgagat gagagacaac
     2041 atatgcgcgt atctctgcgt tcttcagacg gtcggttctt ccacgatgtt tacggtggat
     2101 tagcgctcgt tcgtgagcta aacgggaagt atataccttg ttctctttag gaaagttgcg
     2161 agtcatctct cttgagacag aggagggtga tcttccgata gcatttgcta tcgttcgaat
     2221 cgattctcgc ttccaccaca ttaactgtat tttctctctt tcttcaatag aaaaatgtct
     2281 atattgcata tcactctaga ttagcagagt gttgcactta cttgt
//
LOCUS       DAOTEX010000088         2295 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3118
            NODE_39216_length_2295_cov_4.763393, whole genome shotgun sequence.
ACCESSION   DAOTEX010000088 DAOTEX010000000
VERSION     DAOTEX010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868142
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 883
            CDSs (total)                      :: 833
            Genes (coding)                    :: 829
            CDSs (with protein)               :: 829
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_39216_length_2295_cov_4.763393"
                     /isolate="AMR_MDS_3118"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            107..181
                     /locus_tag="P5056_04300"
     tRNA            107..181
                     /locus_tag="P5056_04300"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:139..141,aa:Gly,seq:ccc)
     gene            complement(410..907)
                     /locus_tag="P5056_04305"
     CDS             complement(410..907)
                     /locus_tag="P5056_04305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62960.1"
                     /translation="MSTNNVLEKLKTFKTIEIGTYTDVKTVLKAMEEIGAGSSYWAGS
                     ILNKTELSKSKQSLDLVVPSVRELGFPNGAMRMKIYEAGRNQGLDLSPAEVGPQLLLQ
                     YPDQPIGTGLVIAMEPITDSDSGLFLFLFLVCNYDGGRYLDAFDGRPDRFYYPNDRFV
                     FVRRK"
     gene            complement(1405..>2295)
                     /locus_tag="P5056_04310"
     CDS             complement(1405..>2295)
                     /locus_tag="P5056_04310"
                     /inference="COORDINATES: protein motif:HMM:NF012307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reverse transcriptase/maturase family protein"
                     /protein_id="HRY62961.1"
                     /translation="MRNLFQLHEDLKTKKYEHSKYVEFKISDPKPRIIHKAAVRDRLL
                     HHALHRMLYPFFDTKFITDSYSCRIDKGSHEALNKFRRFSFKVSKNNTKTAWILKCDI
                     KKFFASIDHKILIRIIKDSISDKDITWILSEVINSFNLGNNKGLPLGNLTSQLFANIY
                     MDRFDKFIKHTLKVKYYIRYADDFIFISENKNELMGMYPKIKDFLWQKLRLELHGSKT
                     LLKSVASGIDFLGWVNFSDHRILRTTTKKRMFRNIVFSNYDQKTIQSYLGLISHGNTY
                     KLRKEIFNQAKPTFLEWSEL"
BASE COUNT          703 a          465 c          416 g          711 t
ORIGIN      
        1 ttcgctcgag aaccttgcaa tcgagccata gcgcttcgca ctatagcatc gatagtaaat
       61 cgtctgcatt ttgttagttt taaaagttaa tacctaaatt agtttcgcgg gcgtggctta
      121 gtggtaaagc gtctccttcc caaggagaag atcgcgggtt cgaatcccgt cgcccgctcc
      181 agatgtttga agttccgata gacccgtctc cctaccaaga agtctaaatt tccatgtgat
      241 ccgagttctc tttccttaaa tatcataata atttggtttt tttaacaaaa aaactccgtg
      301 ctttggtata taaaacccaa gaacacggag ttaagtaaaa cgagctcaaa gtacaaagat
      361 caaagaatct aaattaaaaa aaccaagtga ccagagttca aaggtcaaat tacttacgac
      421 gcacgaaaac aaagcgatcg ttagggtagt agaatctgtc aggcctacca tcgaacgcgt
      481 caaggtaacg accaccatca tagttacaga ccaagaacag gaataggaac agaccgctat
      541 cggagtctgt tataggttcc atggcaatta cgagccccgt accgataggc tggtcaggat
      601 actgcagtag cagctgcgga ccaacttcag caggagacag gtccaaacct tgatttctac
      661 ctgcctcata tatcttcatg cgcatagccc cattgggaaa tcccagttct ctgactgacg
      721 gaaccaccag atctagggac tgcttagatt tgctcaactc tgtcttattg agaatagaac
      781 cggcccaata cgaagaccct gctccaatct cctccattgc tttaagaaca gtcttcacat
      841 ccgtataagt gccgatttca atagtcttga aggttttaag cttttccaaa acattatttg
      901 tactcatgac aattaatcct ttctctacct cattttggta gttatagtaa gatgcgccta
      961 ctatactata ggtgtgaata aaattcaaat aatacaataa cctctttttt atactgtctc
     1021 atttgtctca ttcgtagcct ggagagtata ctctactcgg ggtttccaga ctcgactggt
     1081 gctccatgaa cttgaccagc accttttggt ggtgggcaag ttcagggcgt aagggatgag
     1141 aatgccggag cgaacaaagt gagcgaggcg gggtcgcgtg ctttagagac tcttgcgaat
     1201 gtaatgagtt agagtgtctt aagacaggtg gccgaatccc gtcgcccgct ccagaagttc
     1261 aaagttccga tagacccgtc gccctaccga gaagtctaaa gttccatgtg actcgaatgc
     1321 cctttcctta aatatcataa taatttcgta tgattttcgt atcccagaga agtatttaca
     1381 gacccgtcgg accctaggtg aagttcaaag ttccgaccac tctaaaaatg ttggtttggc
     1441 ttgattaaaa atttcttttc tcagtttgta agtatttccg tgagagataa gccctaagta
     1501 agattgaata gttttctgat cataattact aaatacaata tttcggaaca tcctcttttt
     1561 tgtcgtcgtt cttaatattc tatgatctga aaaattaacc caaccaagaa aatctatccc
     1621 agaagctaca ctttttaaca aagttttgct tccatgcagt tctaaacgaa gtttttgcca
     1681 tagaaaatct ttgatcttag gatacattcc cattagttcg tttttatttt ctgatataaa
     1741 aataaaatca tcggcgtaac gaatataata ttttactttc aaagtatgtt ttataaattt
     1801 atcaaatcta tccatataaa tatttgcaaa aagctgagat gtaagatttc ctagaggaag
     1861 acccttatta ttacccaaat taaaactatt aataacttct gaaagaatcc aagtaatatc
     1921 tttatcggaa atggaatcct taataattct aataagaatc ttgtgatcta tggatgcaaa
     1981 aaacttcttt atatcacact ttaagatcca tgcagttttt gtattattct ttgaaacctt
     2041 aaatgaaaat ctacgaaatt tattaagggc ttcgtgcgaa cctttatcta ttcggcatga
     2101 atatgagtcg gttataaact ttgtatcaaa aaatggataa agcattctat gtaaagcatg
     2161 gtgaagtaat ctatccctaa ctgcagcttt atgaataatc ctcggttttg gatcagaaat
     2221 tttaaattct acatattttg agtgttcata tttttttgtt tttaagtctt cgtgcagctg
     2281 aaacagattt ctcat
//
LOCUS       DAOTEX010000089         2268 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3118
            NODE_40012_length_2268_cov_4.996385, whole genome shotgun sequence.
ACCESSION   DAOTEX010000089 DAOTEX010000000
VERSION     DAOTEX010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868142
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 883
            CDSs (total)                      :: 833
            Genes (coding)                    :: 829
            CDSs (with protein)               :: 829
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40012_length_2268_cov_4.996385"
                     /isolate="AMR_MDS_3118"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..897
                     /locus_tag="P5056_04315"
     CDS             <1..897
                     /locus_tag="P5056_04315"
                     /inference="COORDINATES: protein motif:HMM:NF015113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HRY62962.1"
                     /translation="ITMLEEAGLKNLSVQINSIGDKSCRGSYKRDLISYYKKHANDIC
                     KNCKLRLKTNPLRLLDCKDPKCQPTKEKAPQAISYLCDPCKQHFKEVLEYLEILGIEY
                     VINNNLVRGLDYYTRTVFEIVSKTETQKAEKKEGEVPAEGEPKKEEIEGKEEALAICG
                     GGRYDYLAKSLGNKKDVAGVGLGIGVDRVVMSKGFESTMPRIIKKPKIYFIQLGFSAK
                     LKSLSIIEILRKAKIGVAHSLSKDSLGAQLATAEKMKIPYAIILGQKEVMDGTVIVRN
                     MDDRSQEEIKVEKLGEYLKKLK"
     gene            947..1189
                     /locus_tag="P5056_04320"
     CDS             947..1189
                     /locus_tag="P5056_04320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62963.1"
                     /translation="MILIQVTKSGSESPTGLIRRFSKRVQESGVIRRAKSLRYNQRTL
                     SEYKRKMAALKRLENRQRIEKLKKLGKLKDAPRKRF"
     gene            1167..1559
                     /gene="ybeY"
                     /locus_tag="P5056_04325"
     CDS             1167..1559
                     /gene="ybeY"
                     /locus_tag="P5056_04325"
                     /inference="COORDINATES: protein motif:HMM:NF014216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HRY62964.1"
                     /translation="MLLVRDFKTNTKISKIGYEKIADFALGKKYELSLVFIGPKKMRG
                     LNSKFRQKDRPTNILSFPLSKTEGEIFICPAIAKEEIKIVKRPLGEYIKYLFIHGITH
                     LKGFDHGSKMEKEENKIREKFLKENFSI"
     gene            1596..>2268
                     /locus_tag="P5056_04330"
     CDS             1596..>2268
                     /locus_tag="P5056_04330"
                     /inference="COORDINATES: protein motif:HMM:NF014541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62965.1"
                     /translation="MKKTITTGIDVGTSSVRITVSEHVKGEELPRILALVNKRSSGLR
                     HGYITNIDDITKTIKEAAAEAERIAKVKIEYAYAGIGGISLESSVANGSIEIEHEDKE
                     VRAEDIKKVSEASEANLRRATNKHVLQTFPIGYKIDGRKTLGKPLGMKGSTLEVKTLF
                     ITCQEQHVQSLVSAIENAGIMLEDVVASPIAASFVTVTRVQKTAGCVLVDVGAETVSI
                     AVFEDG"
BASE COUNT          829 a          438 c          514 g          487 t
ORIGIN      
        1 atcacaatgc ttgaggaggc gggattaaag aatctctctg tccaaataaa cagtatcgga
       61 gacaaatctt gtagaggctc atacaaaaga gatcttattt cttactacaa aaaacacgca
      121 aacgatattt gcaaaaactg caaactgaga ctgaaaacaa acccgctccg gctcctcgac
      181 tgcaaagatc caaaatgtca gccgacaaaa gaaaaagccc cgcaggcaat tagctaccta
      241 tgcgaccctt gcaaacagca cttcaaagaa gttttggagt atttggaaat actgggaata
      301 gagtatgtta taaacaataa ccttgtgcga ggtcttgatt attatacaag gaccgtattt
      361 gagattgtct caaaaaccga gacccaaaaa gcggaaaaga aagaaggaga agtcccggcg
      421 gaaggagagc caaagaagga agagatagag ggaaaggaag aggctctcgc aatctgtggc
      481 ggaggcagat atgattattt ggccaaatcg ctaggcaaca aaaaagacgt cgccggcgta
      541 gggctcggta ttggagtaga tagggtggtg atgtcaaagg gattcgaatc tacgatgccg
      601 agaatcatca agaagcctaa gatatacttc atccagcttg gattcagcgc caaactaaaa
      661 agcttgtcta tcatagaaat actaagaaaa gccaaaatcg gtgtggccca ttctctatca
      721 aaggatagtc tcggcgccca acttgcaaca gccgaaaaga tgaaaatacc ttatgctata
      781 attctcgggc agaaagaggt catggatgga acggtaatag tcagaaacat ggacgacaga
      841 tcccaggaag agataaaagt cgaaaaattg ggagaatatc tgaaaaaact aaaataacca
      901 ttagacagtt gcgttacctg caacaatatg ctatcttaaa gaccatatga ttttaattca
      961 agtaacaaaa agcggcagcg aaagcccgac tggcctcata agaaggttca gcaagagagt
     1021 tcaagagtcc ggagttataa gaagagccaa aagcttgaga tataaccaga gaacactttc
     1081 tgaatacaag agaaaaatgg ccgctctaaa aagattagaa aacagacaaa gaatagagaa
     1141 gctaaagaag cttggtaaat taaaggatgc tcctcgtaag agattttaaa accaatacaa
     1201 aaatatcaaa aatcggctat gaaaaaatag ccgattttgc tttgggcaag aaatatgaat
     1261 taagtctcgt attcatcggc ccgaaaaaga tgcgggggtt gaattccaaa tttagacaaa
     1321 aagacagacc gacaaatatt ttgtctttcc ccctatcaaa aacagaggga gagatattta
     1381 tctgccccgc aatcgcaaaa gaagaaatca agatagtgaa aagaccgctc ggcgaatata
     1441 taaaatattt atttatccac gggataactc atttgaaagg ttttgaccat ggtagtaaaa
     1501 tggagaagga ggagaacaaa attagagaga aatttttaaa ggaaaatttt tctatttaaa
     1561 aaactctccc ccttaccagc tattaaaaac ggagcgtgaa aaaaacaatc acgaccggca
     1621 ttgatgtcgg cacatcatct gtgaggatta ccgtttccga acatgtaaaa ggagaagagc
     1681 tcccgagaat tttagccttg gtgaataaac gctctagcgg gttaagacat ggatacatca
     1741 caaatattga cgacatcacc aaaaccataa aagaggccgc agctgaagcc gaaagaattg
     1801 caaaagtcaa aatagaatat gcttatgcgg gtatcggcgg catcagtctt gaatcatcgg
     1861 ttgcgaatgg atccatagag atcgagcacg aagacaaaga agtccgcgcg gaagatataa
     1921 aaaaagtttc tgaggcgagc gaagcaaatc tgagacgagc cacaaacaaa catgttcttc
     1981 agacgttccc tatcgggtat aaaatagacg gcagaaagac tctcggcaaa ccgcttggga
     2041 tgaagggctc tacccttgaa gttaaaactc tatttataac ctgccaagaa cagcatgttc
     2101 aatccctcgt gtccgctatc gaaaacgcgg ggataatgct tgaagatgtt gtcgcctccc
     2161 ctatcgcggc cagtttcgta acagtcacga gagtccagaa gaccgcaggg tgcgtgcttg
     2221 tggatgtggg cgcagagaca gtttctatcg ccgtattcga agacggca
//
LOCUS       DAOTEX010000090         2268 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3118
            NODE_40016_length_2268_cov_3.715770, whole genome shotgun sequence.
ACCESSION   DAOTEX010000090 DAOTEX010000000
VERSION     DAOTEX010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868142
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2268)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2268)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 883
            CDSs (total)                      :: 833
            Genes (coding)                    :: 829
            CDSs (with protein)               :: 829
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2268
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40016_length_2268_cov_3.715770"
                     /isolate="AMR_MDS_3118"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..614)
                     /locus_tag="P5056_04335"
     CDS             complement(<1..614)
                     /locus_tag="P5056_04335"
                     /inference="COORDINATES: protein motif:HMM:NF015639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HRY62966.1"
                     /translation="MKIKMDHPAVRDFCTKLLQRPVEISAMEISQSLFKCQRCGGCCT
                     KMNGISAQKDDVKKMAKELKHSYNWVMSNYVKNGLIRGTSESSPGAADGRCPFYKEGC
                     TVYKGRPIVCRLFPLINFENGKLKPHWYDDCPGTRELIQKIYDYKMKHPELPEYSREE
                     IAAVNALRIMAHLNIMEGIGQIKRYGPIKKLWPRWDLGTHHGIE"
     gene            complement(611..1462)
                     /locus_tag="P5056_04340"
     CDS             complement(611..1462)
                     /locus_tag="P5056_04340"
                     /inference="COORDINATES: protein motif:HMM:NF012524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HRY62967.1"
                     /translation="MLFLEDHSTKSIKVDSISSAYRNALKTIIYNGTNFNDERGDKMR
                     VFYNLSVKVKNPLENLEKLEEMNKKRLISYNREFLDNYANQLIYGVSSPIFKEIEYTL
                     PNKGAFKRILDYFDLNKRVRIQDVRPLPDEDLGFVYTYFERLRKRWSRLAKVTFQSFE
                     NDKIVDSEKMDQIEEVIRLLIKSPTTRRAVATTWIPSIDLRSSEVPCLNWLKFYNTGV
                     LNLSVAFRSHDIFGAYISNLYGLSRLLKYVSIRTGIEMGSLTVFSADAHYNTLFEKDV
                     FRIVEDV"
     gene            complement(1473..1682)
                     /locus_tag="P5056_04345"
     CDS             complement(1473..1682)
                     /locus_tag="P5056_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62968.1"
                     /translation="MNYASVEMFPNNFVITVFDENATLLDSFYFGNHESCESVNRRIK
                     EIEEQFHIEKWVSSQERKMIERQNL"
     gene            complement(1684..1878)
                     /locus_tag="P5056_04350"
     CDS             complement(1684..1878)
                     /locus_tag="P5056_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62969.1"
                     /translation="MFFKAGDTVLILKGEDFDGNDLTGKKAIIANPMPPSFKDATIEV
                     EGSGYQMFFPKEWLKVIGRE"
     gene            complement(1878..2090)
                     /locus_tag="P5056_04355"
     CDS             complement(1878..2090)
                     /locus_tag="P5056_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62970.1"
                     /translation="MNEKKLKAAISEAERFLSRAKELGKLLSEQEKKRIPGEIFYRDH
                     FPKECGSVRRASMDLTRALSDLRRYD"
BASE COUNT          645 a          512 c          376 g          735 t
ORIGIN      
        1 tcttcgattc cgtgatgagt acctagatcc cacctgggcc acaatttctt gatgggtccg
       61 tatcttttga tttgtccaat gccctccatt atattcaagt gggccataat tcgcagggcg
      121 tttacggctg ctatttcttc tcttgaatat tctggcaatt ccggatgttt cattttgtaa
      181 tcataaattt tttggatcag ctctcttgtt cctgggcagt catcatacca atggggcttc
      241 agcttcccat tttcaaagtt tataagcgga aacaggcgac aaacgatggg ccttcctttg
      301 taaaccgtac agccttcttt gtaaaatgga catcttccgt ccgctgcccc aggtgaagat
      361 tcggacgttc ctcttataag tccgttcttt acataattag acataaccca gttatagcta
      421 tgttttaact ccttagccat ctttttaacg tcgtctttct gggcgcttat tccgttcatc
      481 ttggtacagc aacctccaca tctctgacat ttaaaaagag attgagaaat ttccatagca
      541 gaaatttcta ctggcctctg aagaagcttt gtgcaaaaat ccctaacggc gggatgatcc
      601 atttttattt tcatacatcc tccacaattc taaaaacatc tttttcaaaa agcgtattgt
      661 aatgggcgtc agcagagaaa actgttaaag agcccatctc tattccagtt ctgattgata
      721 catatttcaa taaccttgaa aggccgtata ggttagatat gtaggcccca aaaatatcat
      781 gagatcgaaa cgccacgcta agatttaaaa ctccggtgtt ataaaattta agccagttaa
      841 gacaaggaac ctcagagctt cgcaggtcta tggacggaat ccaggtagtt gctactgccc
      901 tgcgagtcgt cggacttttt attaaaagtc tgatgacttc ttctatctgg tccatcttct
      961 cggagtctac aattttatcg ttttcgaaag attgaaatgt tacttttgca agcctagacc
     1021 acctcttccg gagacgctca aagtaagtat agacaaagcc taaatcttcg tcggggagag
     1081 gacgaacatc ctgaattcgt actctcttat tcaaatcaaa ataatctaag atccttttaa
     1141 atgcgccttt attgggcagg gtatattcta tttctttaaa aatcgggctt gagaccccat
     1201 agattagttg atttgcataa ttatcaagga actcgcggtt gtaggaaatc agtctctttt
     1261 tattcatctc ttccagcttt tctaaattct ctaaaggatt cttaacttta accgagaggt
     1321 tataaaagac tctcatcttg tctccccgct cgtcattaaa attcgttccg ttgtatataa
     1381 tagtttttag agcatttcta tatgcggaag agatcgaatc tacttttata gatttggttg
     1441 aatggtcttc taaaaagagc attgggaagt cctcataagt tttgtctttc gatcatcttt
     1501 ctttcctgag atgaaaccca tttttcgata tgaaactgtt cttctatttc ttttatacgc
     1561 cgatttacag actcgcaaga ctcgtgattt ccaaaataaa acgaatccaa aagagtagca
     1621 ttttcatcaa aaacagtaat tacaaaatta tttggaaaca tttctacaga agcataattc
     1681 acttcactcc ctcccaatca ccttcaacca ctcctttgga aaaaacattt gataaccgga
     1741 cccttctacc tcaattgttg cgtctttaaa agaaggaggc ataggattgg caattatagc
     1801 tttctttccg gtcaggtcgt ttccgtcgaa atcctctccc ttcaaaatta aaactgtatc
     1861 tccggcttta aagaacatta atcgtatctc cttaaatctg aaagagccct tgtcaggtcc
     1921 atacttgccc tccgcacaga accacattcc tttggaaaat ggtctcggta gaaaatttct
     1981 ccaggaattc gtttcttttc ttgttcggaa aggagctttc caagttcctt tgctctgctc
     2041 aagaatcttt ctgcctcaga aatagcagct tttaattttt tctcattcat atgaacctcc
     2101 tccacaaagc cactaagaat attaaaacaa atccaaaaca aaacccaaaa agagtcaaaa
     2161 tagtatcgtc gatcatgaaa accacggcac cttcatttcc caaccttttg actccccatc
     2221 cttacaggta gccagccggt atttccaaaa aatattttct atcacatt
//
LOCUS       DAOTEX010000091         2076 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3118
            NODE_46272_length_2076_cov_2.592776, whole genome shotgun sequence.
ACCESSION   DAOTEX010000091 DAOTEX010000000
VERSION     DAOTEX010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868142
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 2076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 883
            CDSs (total)                      :: 833
            Genes (coding)                    :: 829
            CDSs (with protein)               :: 829
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2076
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_46272_length_2076_cov_2.592776"
                     /isolate="AMR_MDS_3118"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(449..730)
                     /locus_tag="P5056_04360"
     CDS             complement(449..730)
                     /locus_tag="P5056_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62971.1"
                     /translation="MGIDARTATAADFDASAQKHANAFWVWLVITGIIFYFFKWWSVI
                     PAVLAIWAVVSSIGATKAASNLRNGTYRITNPNNGAPDGDARNSNKKDG"
     gene            complement(743..1207)
                     /locus_tag="P5056_04365"
     CDS             complement(743..1207)
                     /locus_tag="P5056_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY62972.1"
                     /translation="MKYASYAWLVIKNIIVLLIAFLLYSKANTSFEIIIVSLLLIIFT
                     HAISSSSVLALGVWELSIKSSKQFLHILQILKRHDREYEGLKEIREKALLENKDYFSG
                     IEKLYYEDEDTKEGMKEQEALHKELTIKSYITETFVFLIYALAVWKIIATLI"
     gene            complement(1316..>2076)
                     /locus_tag="P5056_04370"
     CDS             complement(1316..>2076)
                     /locus_tag="P5056_04370"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008134 - transcription factor binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HRY62973.1"
                     /translation="GADKQRKGLLELADDGIIFLDELGKMPKRLQAKILKAIQDKTFR
                     RVGGNDTFPIKARFIAAVQPDDLKENILPDLKYRFGHPNNITVPSLYDRIKINPRAVI
                     FSALNQVKRSMGVEKGIRINKQAVSLLCNYDYKGNFRELCTILEAGLTEALMDNRNEI
                     LPEDVTDILNTDKNNFVQNAEHELDSIKLKDVFDYAEDIKKTIIEKKISRVLNSGKNI
                     KTALSEEGLNQEKDYQNFRKKVVNITGKNLKELI"
BASE COUNT          589 a          404 c          371 g          712 t
ORIGIN      
        1 agagacccat tgccgacaat gccacgaaag ccggcaaagc cgagaaccgt cgtgtcgaag
       61 cggctgttga ttacatcatc aagaagtagg taaaggaaaa ggtagaagta aaagatagat
      121 tgattatata ggctttaagt tatgaagccc ccgtgcaaaa cgggggctat catcaagtgt
      181 ttcgtttcca atcttaacgg cggccacttt tcaatctgtt ctctccttga ttgtaagtgg
      241 tttacgctcc cgttcggtca gcctgccgtt cgggagcgtt ctttttgcac ctttcaaatc
      301 gtaattcaca acgtcaggac ttttaaaaat cgtttagttg taggtgtaac ggttgtcaag
      361 acaagaccta attggatagt gtctgtcaag ttaacaatag gtcaggacaa ttaatataca
      421 ataggaatta aaaaagcaga ttattatttt agccatcctt tttgttggaa tttcttgcat
      481 ctccgtctgg cgcgccatta tttgggttag tgattctata ggtaccattt cgaagattag
      541 aagccgcttt tgtagcacca atgcttgaga caactgccca aatagctaat acagccggga
      601 taaccgacca ccatttaaaa aagtaaaaaa tgatgccggt aatcaccagc catacccaaa
      661 aagcatttgc gtgtttttga gccgatgcat caaagtctgc tgccgttgca gttcttgcgt
      721 caattcccat atccactccc aattatatta atgttgctat aattttccaa accgccaatg
      781 cataaattaa aaaaacaaac gtctccgtaa tatatgactt tatggttagt tctttatgca
      841 gagcctcctg ttctttcatt ccttcttttg tatcttcatc ttcgtaatat aatttttcga
      901 ttccactaaa ataatcctta ttctccaaga gtgctttttc tcttatttct ttcaatcctt
      961 catattcgcg atcatgtctt ttgagaattt gtaatatatg gagaaattgt ttgcttgatt
     1021 ttatgctcaa ttcccatacg ccaagagcaa gaacagatga agaactaata gcatgggtaa
     1081 agataatgag taacagggaa acaattatta tttcaaaact ggtattggct tttgaataaa
     1141 gcagaaatgc aataagtaat actatgatat tcttgataac aagccaagcg taagaagcat
     1201 atttcataat atccctgtca ttctgtcgtt atttgtcttg atgagttttg ttttgttatt
     1261 tttatatgag attaataact tgtatgtcaa taaaatatta tttgaaagac attgattaaa
     1321 ttaattcttt taagttcttg cctgtgatgt tcacaacctt cttccgaaaa ttttggtaat
     1381 ctttttcttg attcagccct tcttcagaaa gggcggtttt aatgtttttg ccactattca
     1441 atactctaga aatctttttc tctataattg ttttttttat gtcttcggca taatcaaaaa
     1501 catctttcag cttgatggaa tcgagttcat gttcggcatt ctgaacaaaa ttatttttgt
     1561 cggtatttaa aatatcagtc acatcctcgg gcaagatttc gtttcggtta tccattaatg
     1621 cctctgtcaa tcccgcttct aaaatcgtgc ataattccct aaaattgcct ttgtaatcat
     1681 agttgcacaa taaagaaact gcttgcttat ttatgcgaat acctttttcc acgcccattg
     1741 accgtttcac ttgattgaga gcggaaaata tgacagctct cggattgatc ttgatacggt
     1801 catataaact aggaactgta atgttgttag gatgcccaaa cctgtatttc aaatctggca
     1861 agatattttc tttgagatcg tccggttgaa cggcggcaat aaaacgggct tttataggga
     1921 aggtgtcatt gccccccact cttcggaagg ttttgtcttg aatggccttt aatatctttg
     1981 cttgcaaccg tttaggcatt ttcccgagtt catcgagaaa tattatcccg tcatctgcca
     2041 attctaaaag tccttttctt tgtttgtctg ccccgg
//
LOCUS       DAOTEX010000092         1868 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3118
            NODE_55334_length_1868_cov_3.801434, whole genome shotgun sequence.
ACCESSION   DAOTEX010000092 DAOTEX010000000
VERSION     DAOTEX010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868142
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 1868)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1868)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 883
            CDSs (total)                      :: 833
            Genes (coding)                    :: 829
            CDSs (with protein)               :: 829
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1868
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55334_length_1868_cov_3.801434"
                     /isolate="AMR_MDS_3118"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            230..502
                     /gene="rpmA"
                     /locus_tag="P5056_04375"
     CDS             230..502
                     /gene="rpmA"
                     /locus_tag="P5056_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HRY62974.1"
                     /translation="MAHTKAGGTAKNLRDSQPKYLGTKLYGGETAKAGSIIIRQRGTV
                     ILPGANVKLGKDHTIFALKTGKVKFSTKRKVHFDGTTKKKVTVSVE"
     gene            complement(593..1036)
                     /gene="rplI"
                     /locus_tag="P5056_04380"
     CDS             complement(593..1036)
                     /gene="rplI"
                     /locus_tag="P5056_04380"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HRY62975.1"
                     /translation="MKIILLKDVPKIGRRYEIKEVSDGYARNFLLKNGLGEIATDAIV
                     RKMDEEKKKTEADKKNREAKVNEKIASISKEPIVIESKANEDGHLFAGIKKEEVLKLI
                     TDRGAEIKPEELEMNKPIKEIGPHEVSVKISGKTAKIKIEVIKKN"
     gene            complement(1224..>1868)
                     /locus_tag="P5056_04385"
     CDS             complement(1224..>1868)
                     /locus_tag="P5056_04385"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRY62976.1"
                     /translation="TSDGKPIKGNNSGNGLREDGVFVVRLFNFSAPSADLFRDALRKF
                     IFSGSDKLVLDLRGNPGGYLEAAVDMASWFLPSGKVIVSEEFGTNSNQAPNIHRSKGY
                     NIFDPKVLKMAILIDKGSASASEILAGALNEHGVAKLVGEKSFGKGSVQELIPVTGDT
                     FLKITVAKWMTPNGHSISDFGLTPDIIVPITKEDIAAGKDPQLERAAKLLISGK"
BASE COUNT          506 a          386 c          347 g          629 t
ORIGIN      
        1 ttgtagccct tgattttttc tccaatatca tttagtattc ccatatcgtt gtttggaacc
       61 aatataccat tatatatatt ccaggcaaat ttcaccctgt taagtagatt ttctgaaaga
      121 aaatcaaaat ttgttaaaaa caaataagca gggtgcacga tgcgaaggac attacaataa
      181 ataatcatta ggtaaaaaca attaaacaaa tttttactta acagggtaaa tggcacatac
      241 aaaagccggc ggaacagcta aaaatttaag agattcacaa ccaaagtact taggtactaa
      301 gctttatggt ggagagaccg cgaaagcagg atctatcata atcagacaga ggggaacagt
      361 tatcttgccc ggagcaaacg taaaactcgg caaagatcac acaatcttcg cactaaagac
      421 aggtaaggtt aaattcagca caaagagaaa ggttcatttt gacggaacaa ctaagaagaa
      481 ggttaccgta tctgtagagt aagttcccta aacccaacat ataaatttca aaagaaaaat
      541 cacattcgac ctgaatgtga ttttttaatt cgtaattcgt aattcctaat ttttaattct
      601 tttttatcac ttctattttt atcttcgcag ttttgccgct tatttttaca gaaacttcgt
      661 gtggtccgat ctcttttatc ggcttattca tctccagttc ctcgggtttt atctctgcgc
      721 ctctgtctgt tatcagcttc aagacctctt ctttttttat gccggcgaat aggtggccgt
      781 cttcgtttgc ttttgattct ataactatcg gttctttgga aatagaagcg atcttttcat
      841 taacttttgc ttctcggttc tttttgtcgg cttccgtctt tttcttctct tcatccattt
      901 ttcttacaat cgcatcagtc gcaatctcgc caagaccgtt ttttagcaaa aagtttctgg
      961 catatccgtc ggacacttct ttaatctcgt accttcttcc tatttttggt acgtctttga
     1021 gtagaattat tttcacttgc cctgttaagt aaaaattact ttttacttat ttaattatat
     1081 tgttaaatta tcgactattt taaattctcc cgattaattc accgaaagta attttatccg
     1141 gttgagtaaa attagctccg ctaatttaaa ttttattttt agataaaatt ttactcaatc
     1201 ggattactta acagggtgaa tttttactta ccgctaatta gtaatttggc cgccctctct
     1261 aattgagggt ccttgcccgc ggctatatct tctttagtta taggcacaat gatgtccggg
     1321 gtcaagccga agtcggagat agagtggccg tttggcgtca tccatttggc gacggtaatc
     1381 ttcaagaatg tgtctccggt gacgggtatt agctcttgaa ccgatccttt tccgaatgat
     1441 ttttctccta caagtttcgc aacgccgtgc tcgttcaatg ctccggctaa tatttcagat
     1501 gcagaggctg aacctttatc tataagtatg gccattttaa gaacctttgg atcaaagata
     1561 ttatatccct tgcttctatg aatattcggc gcctggttgc tgtttgttcc gaactcttcc
     1621 gaaacaatga cttttcctga aggtaagaac cagctcgcca tatctacggc cgcctcaaga
     1681 tatccgccgg gatttccgcg taagtctagg accaatttgt ccgaaccgga gaatatgaat
     1741 ttcctgaggg cgtcgcggaa gaggtctgcg gatggggcgg agaagttgaa aagtctgacc
     1801 acgaatacgc cgtcttcgcg gagtccgttg ccggaattat ttccttttat cggttttccg
     1861 tcggacgt
//
LOCUS       DAOTEX010000093         1867 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3118
            NODE_55385_length_1867_cov_4.050221, whole genome shotgun sequence.
ACCESSION   DAOTEX010000093 DAOTEX010000000
VERSION     DAOTEX010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868142
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 1867)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1867)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 883
            CDSs (total)                      :: 833
            Genes (coding)                    :: 829
            CDSs (with protein)               :: 829
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1867
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_55385_length_1867_cov_4.050221"
                     /isolate="AMR_MDS_3118"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..535)
                     /locus_tag="P5056_04390"
     CDS             complement(<1..535)
                     /locus_tag="P5056_04390"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HRY62977.1"
                     /translation="MGQTKKDKIYIYGRHALKEALTNAPQAVTKIFLSDRDDAELEAI
                     IKKTGTPVGELNIANIEKGASHQGVIASVAPDKLVIKYEEFFNSYKVSKTSLFVFLNG
                     LQDPQNVGAIIRSAVAFGASGVIMPEKEQAPITGAVAKTSAGMVFRVPIISAGSKLDT
                     IKDLKKRGFVIYGMEGES"
     gene            664..>1867
                     /locus_tag="P5056_04395"
     CDS             664..>1867
                     /locus_tag="P5056_04395"
                     /inference="COORDINATES: protein motif:HMM:NF023650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan binding domain-containing protein"
                     /protein_id="HRY62978.1"
                     /translation="MQLIINVSRNIATYIIIILAVFGIVGFEYKYQDKFYPGVYVDGK
                     SVGGKTYEEVLSEYKKGADVLYKDGITLVFEGQNDSREVNIPMVVSGMSSYGVVEYFS
                     LGGWENTIKEAYDYGRNGSALTKAKEQTSSLFSKKTFDFPAVFQNEAVRSFFSRELKD
                     HFIESVPAEFSYVGGNIVVSKESAGEGVNFDAVAEEINKKLSSFDPSPIILKSEKIEP
                     EFTKEQLEMFLPVAKKISRGINLYFKYNGYGWRVSGRKLVTWLKLDNEGRLSLDRIKA
                     EAFLSKNIIPVLDNKPLSSRFKMKDGILVETEPGKPGNMVDVEGTIAKVEEVIFGKKR
                     SLGLADSSLASLGALAVGNSDIVLEDGYIIVPIDLKVEQPRVTKDTLGQYEIRELVGT
                     ATTNFKGSS"
BASE COUNT          532 a          381 c          417 g          537 t
ORIGIN      
        1 ttgattcccc ttccattcca tatataacaa aacccctctt cttaagatct tttattgtgt
       61 ctaacttact tccggcgctt ataatgggca cgcgaaatac catccctgcg gaggttttgg
      121 ctactgcgcc tgttatagga gcctgttcct tctccggcat tatcactccg ctcgccccaa
      181 acgctacggc tgagcgaatt atcgccccga cattttgagg gtcttgcaga ccgtttaaga
      241 aaacaaaaag tgaggtcttg ctgaccttgt aactattgaa aaattcctca tattttataa
      301 cgagtttgtc gggggcaaca cttgcgatta ctccctgatg agaagcgccc ttttctatat
      361 ttgcgatatt taattctcct acaggagttc ctgttttctt tatgattgct tcaagctccg
      421 catcatctcg gtctgaaaga aatatttttg ttaccgcttg cggcgcgttt gtgagggcct
      481 ctttcaaagc atgtctcccg taaatatata ttttgtcttt ttttgtttgt cccatgaaat
      541 tggctaaacg tttctacaaa aatgataata atctgcttaa attaattttt atgccgttgg
      601 cgtcgggttt attttaccac aaagggcttc ttttgtttcc acaaaaaaga taaaatagaa
      661 tacatgcaac taattatcaa tgtttccagg aatatcgcga cctacataat aataattttg
      721 gcggtatttg gaatcgtcgg ttttgaatat aagtatcagg acaaatttta tccgggcgtt
      781 tatgtggacg gaaagtctgt gggcggcaaa acatacgaag aagtattaag cgaatacaag
      841 aaaggcgccg atgttcttta taaagacggc attactctag tttttgaagg gcaaaatgac
      901 tcgcgcgaag taaatattcc gatggtagtc agcggaatgt cgtcttatgg cgtcgttgaa
      961 tatttttctc tcggaggatg ggaaaatacg ataaaagagg cctatgacta tggtaggaat
     1021 ggaagtgcgc taacaaaggc caaagaacaa acatcgtcac ttttttcaaa gaagacattt
     1081 gattttccgg cggtattcca gaacgaagcc gttcgttcat ttttttctcg agagcttaag
     1141 gaccatttta tagaatcggt tccggctgaa ttttcttatg tcggcggtaa tattgtggtc
     1201 tcaaaagaaa gtgcgggaga aggagtaaac ttcgacgcgg tggctgagga gataaacaaa
     1261 aaactttcct cttttgaccc ctccccgata attttgaagt cggaaaaaat tgagccggag
     1321 tttacaaaag agcagctcga aatgttcttg cctgttgcaa aaaaaatttc acgcggaatt
     1381 aatctttatt tcaaatataa cggctacggc tggagagtga gcgggcgaaa acttgtaacc
     1441 tggctgaaac tagacaacga aggtcgtctt tctttagaca gaatcaaggc cgaagcattt
     1501 ctttccaaaa acattattcc ggttttagac aacaaaccgc ttagtagccg atttaaaatg
     1561 aaagatggca tccttgtcga aacagaaccc ggcaaaccgg gcaatatggt ggatgtagag
     1621 ggcactatcg ccaaggtgga agaggttatc ttcggcaaaa aacgctcgct tggccttgcg
     1681 gattcgtctc tcgcctcgct cggcgcactc gctgtgggca acagcgacat agtcttggaa
     1741 gatggatata taatagtccc aatcgacctt aaggttgaac agccgcgagt cacaaaagac
     1801 acgctggggc aatatgaaat cagggagctc gtgggcacgg ctacgacaaa tttcaaagga
     1861 agcagtg
//
LOCUS       DAOTEX010000094         1698 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3118
            NODE_65362_length_1698_cov_4.261108, whole genome shotgun sequence.
ACCESSION   DAOTEX010000094 DAOTEX010000000
VERSION     DAOTEX010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868142
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1698)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 883
            CDSs (total)                      :: 833
            Genes (coding)                    :: 829
            CDSs (with protein)               :: 829
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65362_length_1698_cov_4.261108"
                     /isolate="AMR_MDS_3118"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /locus_tag="P5056_04400"
     CDS             complement(<1..269)
                     /locus_tag="P5056_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRY62979.1"
                     /translation="MPEPLAHKIRPQKLNEFVGQEHLVGENKPLRIAMEGKHLFSFVL
                     WGPPGVGKTTLAKIYANALDVDFYELSAVSAGIAARSVSYFACAAA"
     gene            complement(292..1062)
                     /locus_tag="P5056_04405"
     CDS             complement(292..1062)
                     /locus_tag="P5056_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HRY62980.1"
                     /translation="MDSRLPAQAGLRGDDKRMNTNIIFKAKDIKAALKSAADPTKAKI
                     LKRFFKTGKGDYGAGDKFLGIIVPEQRKIANKFSGISLVETEALLQSKYHEERLTALL
                     ILVAKFNRGNGQERKEVFDIYKKNTDYINNWDLVDLSAPNIIGEYLKEKDRLLLYKYA
                     RSKELWERRIAIVSTFAFIKEGDCKDALKISEMLLKDRHDLIHKAVGWMLREVGKRCG
                     EKILEGFLEKHIKEMPRTMLRYSIERLSDDKKKFYLQK"
     gene            complement(1071..1427)
                     /locus_tag="P5056_04410"
     CDS             complement(1071..1427)
                     /locus_tag="P5056_04410"
                     /inference="COORDINATES: protein motif:HMM:NF025846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRY62981.1"
                     /translation="MKKVSPQFGGKTKKKKLTTVEITKNMLDEGKTLLEVSFERDLSS
                     STVLDHCEKIKLAYPEFNFERLAKIISPARQKKIKEALIKGGKEGGKYLLSPAKNKLG
                     SGYSFEDIRLVRLLLN"
BASE COUNT          401 a          388 c          331 g          578 t
ORIGIN      
        1 gccgccgcgc aggcgaaata cgacacggag cgggcggcga tgccggcgga gacggccgag
       61 agctcgtaga aatctacatc aagggcgttg gcatatatct ttgcaagggt tgtcttgccc
      121 acgccgggtg gcccccacaa tacgaacgaa aaaaggtgtt taccttccat cgctattctt
      181 agaggtttat tttcgccgac gaggtgttct tgcccgacaa attcgttcag tttctgtggt
      241 cttattttat gcgctagtgg ttctggcata attcaatatt aagccgtata tttacttttg
      301 caaataaaat tttttcttat catccgaaag tctttcgata gagtatcgga gcatcgttct
      361 cggcatttct tttatgtgtt tttcaagaaa accttccagt attttctccc cgcatctttt
      421 gccaacttct cttagcatcc agccgacggc tttgtgtatt aggtcgtggc gatcttttag
      481 aagcatctcg gatattttca atgcgtcttt gcaatcccct tcttttatga atgcaaaagt
      541 tgatactatc gcgatccttc tctcccacaa ttctttagac cgcgcatatt tatacaaaag
      601 gaggcggtct ttttctttca aatattctcc aataatattt ggagcggata gatcgacaag
      661 gtcccagtta tttatgtagt cggtgttttt cttatatata tcaaatacct cctttctctc
      721 ttgcccgttt cctctgttaa attttgcgac gagaatgagc agtgctgtga gtctctcttc
      781 gtgatatttg ctttgtagaa gcgcctctgt ttctaccaga gatatgccgc taaatttatt
      841 tgcaatcttt ctttgctccg gcacaataat cccgagaaat ttatccccgg ctccataatc
      901 cccttttcct gttttgaaaa atctttttaa gattttggcc ttcgtggggt cggcagcgct
      961 tttcagggcg gctttaatat cttttgcttt aaatatgata tttgtattca tcctcttgtc
     1021 atcaccgcgg agacctgcct gcgcaggcag gcgggaatcc atagatattt ttaattaagc
     1081 agtagtctaa ctagtctaat atcctcaaaa gagtatccgg agcccagttt attcttggcc
     1141 ggagagagca gatatttccc gccttcttta ccacccttta tgagcgcctc ttttattttt
     1201 ttctgtctgg cggggctaat tatttttgct aatctttcaa aattaaattc gggataggct
     1261 aattttatct tttcacaatg gtctagcacc gtgctggagc tgagatctct ctcaaaagaa
     1321 acttccagca atgtttttcc ttcatccaac atgttctttg ttatctcaac tgtggtcagt
     1381 ttcttctttt ttgttttgcc gccaaattga ggagaaactt ttttcaaaaa gtttccccac
     1441 aagaaagaac aaaaagatcg acaaagcacg aaaggcaaag cgtacgatat tcatcatccc
     1501 aggttacgcc tgggatgttc aatatgttgc ttttttgccc aaaatacccc aaaaaatttc
     1561 tacgggtttt tcttgccata ttttatttta gttcaacccc ggaagtcgtt gaacataccg
     1621 tcaatcctgt tccgcaggtt tcaccaattg ttgggttacg ctgcaatttg ctgggttacg
     1681 ctgcgctaac ccagccta
//
LOCUS       DAOTEX010000095         1541 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Minisyncoccota bacterium isolate AMR_MDS_3118
            NODE_77218_length_1541_cov_3.938089, whole genome shotgun sequence.
ACCESSION   DAOTEX010000095 DAOTEX010000000
VERSION     DAOTEX010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868142
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Minisyncoccota bacterium (wastewater metagenome)
  ORGANISM  Minisyncoccota bacterium
            Bacteria; Minisyncoccota.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:40:27
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 883
            CDSs (total)                      :: 833
            Genes (coding)                    :: 829
            CDSs (with protein)               :: 829
            Genes (RNA)                       :: 50
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 44
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 4
            CDSs (without protein)            :: 4
            Pseudo Genes (ambiguous residues) :: 0 of 4
            Pseudo Genes (frameshifted)       :: 0 of 4
            Pseudo Genes (incomplete)         :: 4 of 4
            Pseudo Genes (internal stop)      :: 0 of 4
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Minisyncoccota bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77218_length_1541_cov_3.938089"
                     /isolate="AMR_MDS_3118"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2053589"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1442)
                     /locus_tag="P5056_04415"
     CDS             complement(<1..1442)
                     /locus_tag="P5056_04415"
                     /inference="COORDINATES: protein motif:HMM:NF021897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HRY62982.1"
                     /translation="MDYKGSLDKLGMTTNRDYIIAEFAFNIGPMNIYVFVAIVLALVA
                     GFFLFRKKKTYNSLNLVLLSVRLPRVVNEDGKKESFLSQINLSEQLFASLASINEPFS
                     FEASVKNTEQQIFFYISVPRKYVDFAMRQIQGLFPEAKVDQVPDYTIFGPSSFAAVAE
                     LTLKESFVLPIRTYKEAEVDTFSPIISTLSKLEEVGEGASIQIVMKPVGKGVKESIVF
                     GIEKLKQGKTIKDVLKDPSLIKGLEKGFREFLFGSHKKYDDAEAVKSPKIIDEDAVKA
                     LYLKSGKQLFTANVRLVASGHTRDSAEDILLGMAGAFSQFSSPMRNEIKIFKPSARHL
                     KKALFKYTFREFDEDSGIVLNTEELASIFHLPTSSTDVPRIWWLKSKEAPPPENLPAE
                     GIMLGESIFRGDRRVVRMTDEDRRRHLYIIGQTGTGKSVTMKNLVVQDMKNGKGVCVI
                     DPHGELINDILGLVPKERIDDVIVFDPGDLT"
BASE COUNT          421 a          368 c          305 g          447 t
ORIGIN      
        1 gtgaggtctc ccggatcaaa cacaatgacg tcatctattc tctcttttgg aacaaggcca
       61 agaatgtcgt ttattagttc tccgtgcgga tctattacgc aaacgccctt gccgttcttc
      121 atatcctgaa cgacgagatt cttcatcgtc acagacttac ctgtgccggt ctgacctatt
      181 atatagaggt gccttctcct gtcttcgtcc gtcattctaa cgacccttct gtcgcctctg
      241 aaaatgcttt cgcccaacat tatgccttca gcagggagat tctccggggg aggggcctct
      301 tttgacttca gccaccagat acgcggaaca tccgtcgagg acgtaggaag atggaatatg
      361 cttgcaagct cctctgtgtt caagacaata ccggaatctt cgtcaaactc tctgaatgtg
      421 tatttgaaaa gcgccttttt tagatgtctg gcggacggct taaatatctt tatctcattt
      481 ctcatcggag aagagaattg agaaaatgct cccgccatgc cgagcaaaat atcttccgcg
      541 gaatctcttg tgtgtcctga ggcgacaagg cgaacgttcg ccgtaaagag ctgtttaccg
      601 gacttcagat aaagagcctt tacagcatct tcgtctatta tctttggaga cttcacagcc
      661 tccgcatcgt catatttctt gtgacttcca aaaagaaatt ccctaaaacc tttttccaga
      721 ccttttatga gcgaagggtc cttaagaaca tcttttatcg tctttccctg ctttaatttt
      781 tcgataccaa aaacaatcga ttctttcacc cctttaccga caggcttcat gactatctga
      841 atagaggcgc cttctccaac ctcttctagt tttgaaagcg tgcttatgat aggggagaat
      901 gtgtctacct ccgcctcttt atatgtcctt ataggcaaaa caaaactttc cttaagggtc
      961 agctctgcga ccgcggcaaa agagctcgga ccaaaaatag tgtaatcggg cacctggtct
     1021 acttttgctt ccgggaaaag tccctggatc tgacgcatgg cgaaatctac atattttctc
     1081 ggaacggaaa tgtaaaaaaa gatctgctgt tcggtgttct tgaccgatgc ctcaaaagaa
     1141 aaaggttcat taatggaagc caatgaagca aaaagctgtt ctgaaagatt tatctggctc
     1201 agaaaagatt ctttcttgcc gtcttcgttc acaaccctcg gaagtctgac tgaaagaagc
     1261 acgagattaa gggaattgta ggtttttttc tttctaaata agaaaaagcc ggccacaaga
     1321 gccaaaacta tagcgacaaa cacatatata ttcataggac ctatattaaa agcgaactcc
     1381 gctattatat aatcacgatt agttgtcatc ccgagcttgt cgagggatcc cttataatcc
     1441 aaactcgaag cgatttctcg acgggctcga aatgacaaaa acgggatata aaatattatt
     1501 taactattcc ctcggccctt agtttctcat acattttatc c
//
