LOCUS       DAOTFA010000001        34657 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate AMR_MDS_3115
            NODE_821_length_34657_cov_7.840732, whole genome shotgun sequence.
ACCESSION   DAOTFA010000001 DAOTFA010000000
VERSION     DAOTFA010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868139
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (wastewater metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 34657)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34657)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:36:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 536
            CDSs (total)                      :: 521
            Genes (coding)                    :: 520
            CDSs (with protein)               :: 520
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34657
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_821_length_34657_cov_7.840732"
                     /isolate="AMR_MDS_3115"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..701
                     /locus_tag="P5014_00005"
     CDS             <1..701
                     /locus_tag="P5014_00005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56530.1"
                     /translation="ISPLLAIIISLALISFSIIYSIIYLDDYFVAMLFIYIAMQFAYS
                     FYLRNVIILDSLIVASGFIIRVFSGGFVSRTSISSWLILTTIGISMLLAFGKRRSEKT
                     ILAKYSVANDDIKTRETLRRYPDTLLDSMISMSASYTILTYSLFTFMVSPGEVGDRIS
                     SFLPTVLKSPKLMMLTIPLVIYGIARYLYVIYEKEEGESPERVLLSDKPLLLTVLIWG
                     LVASVIIFYLPILS"
     gene            complement(698..1054)
                     /locus_tag="P5014_00010"
     CDS             complement(698..1054)
                     /locus_tag="P5014_00010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56531.1"
                     /translation="MRTVFKIGFNTVLLAMFLGMLVLPAGFMGLMSFEENSNVLSAQD
                     SIYEESSIKSIDNPEVPLDVKEMILKMEREYYQEQQDKFVGDINRTDEIEKLNEQTTE
                     NKEETVEENSNVSEKN"
     gene            complement(1054..1623)
                     /locus_tag="P5014_00015"
     CDS             complement(1054..1623)
                     /locus_tag="P5014_00015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56532.1"
                     /translation="MTLSKILARLIDKAIIPAVLLIASRLISIVLVSKYLGVAFKITD
                     RGLIVQNSYDYIKVNSYSVLFMTTMLVIGLFYVLIKSLFFHDSHIKPTITAKLFSIKA
                     ESLIQNSYEIYTQGAVWVSYSYMLLLVSGIMAMSNLMYKWVFYIITGVTLLTTLLFVF
                     DVEEEMKIKKDKDVEYDTDKSLIELPGDL"
     gene            1689..3590
                     /locus_tag="P5014_00020"
     CDS             1689..3590
                     /locus_tag="P5014_00020"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRY56533.1"
                     /translation="MRTIQSIEDILKKPYFLLFSILLFFTPLVFSQNTNELFEFPKMF
                     FVFVIGLFIILFFATDTIIKSKKIISPGVLVVLFLLLTSISTFFSTHKYTSLVGYYSR
                     FNDSLFSYFIYFFLYLICINRITKEDYEKLIKLVLISIIPVSLYGIAQSISGVTRVYS
                     TFGQPNWLSQYLGMLLPMTAYYFFTEKGERFKLWGLVYIFGFYCLWVTFSVSGLLGFF
                     AGVFYLIVKYFRKIFENKVYKTKLIFLISITALIALLNIGLYKEKFYDFYLDVQKQFV
                     LIKKAYASENINRLSDPGFIRLELWKSTFDLITSSPKTFLFGTGPETFPYVFQNYRNG
                     KLNYSSEWDFVFNKPHNYYLEIWSESGILTLGIFIALIYTYVKKLPNHMASAIVVFSV
                     TNIFGWPVVPTSLLFWLFLSGLEINDVKLKPFETETANKLKLNKVRLLYKTLLITLIV
                     GVYLYLNFYIFKFYNADIYYKKGQEYIKKGEEKKALYFADKAVIANPLEPNYYRGRAK
                     VNTVLLVSSQDKQKIKQEIFEDLTKSFELNKNNLVTIRNSIPIYYFLAVGDIYKAISN
                     NNYDERYIGYVKEFYNETKNNYPNDAGVILGVAKYEKKLGLEESYQNSVERIKFLRPD
                     LLDWNELFR"
     gene            complement(3579..4766)
                     /locus_tag="P5014_00025"
     CDS             complement(3579..4766)
                     /locus_tag="P5014_00025"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HRY56534.1"
                     /translation="MAVSTISNDEKPLSTALLYFLGIVGVALVVFFGGELIRNLTGIG
                     NRSALEVRVASGEAQVLINNEVVGNTPFESKDIKPGTNVIAIRSDTRQYQTEIKFLPS
                     KKDTIYTVGIERDLGISDLFSSGRELWFDKDGSDNTLRIVSEPSGADVYVDGSKIGTT
                     PFSSAAITSGDYNLKVALAGYESNETNINIQRGYTLNVSVKLFPFPVDPIVEMFKDSP
                     NLYNISSDNPDITSDTVSWAKGVVYWNTTRGVNIDNVGINKDKLFDYFIDYKGGLFDA
                     NGNPITKQEDFAKLKDLKRGAYLGLKSSGGGISQEAKSALESLSSVGVETVKKEIAKV
                     KPTPLGWLRVRETPSLNGAEITKVNTGESYDVLDKETGWIKIKVSETVEGWVSDAYIE
                     LSE"
     gene            4786..6654
                     /locus_tag="P5014_00030"
     CDS             4786..6654
                     /locus_tag="P5014_00030"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF002436.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HRY56535.1"
                     /translation="MVVKYQHRDFLLKGLKMTTNELKEKFIKFFQERGHSLIPSASLI
                     PENDPTVLFTTAGMHPLVPYLLGEKHPLGNRLVNVQKCLRTDDIDEVGDDTHVTFFEM
                     LGNWSLGDYWKKESISWSFEFLTKELRIPVEKLAVTCFAGDDNAPRDEESAEIWKSVG
                     IPENRIFFLGKIDNWWGPVGSSGPCGPDTEIFYWTGGGEPNNLDSAENCNDWVEIWNN
                     VFMQYEKTSEGKYIPLKQKNVDTGMGVERTIAVLNGKKSVYETDAFLEIYEKVSELSS
                     SDDEISKRIICDHVRASTFLLGDQRCITPSNVDQGYVLRKLIRRSIRKAKKIGINEPF
                     LVSLSKIIIDQYSNDYSELTENQNFIEKYLGLEEEKFNKILNDGQQESFREIEKISDV
                     NEIVNVAGIEVLRAAKISFDLYQSQGYPMEMFVEDMKEKKNIEGSLSEKICEDVGRLI
                     STHQNVSRKGAEKKFKGGLSDNSDDVVRYHTATHLLNKALREVLGDHVKQIGSNITPE
                     RLRFDFPNNSKLTQEEIERVQNIVNDVVDKELPVNFLVCSHEDAVSCGATHLEGEKYP
                     DQVKVYFIGDSLESSFSKEFCGGPHVKNTKDIGHIEIYKQDKIGEGKMRIYARFKS"
     gene            6748..7050
                     /locus_tag="P5014_00035"
     CDS             6748..7050
                     /locus_tag="P5014_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015619077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HRY56536.1"
                     /translation="MTSIEKIKPLSDYLLIEPLEKETVLPSGIVVPDTAKDKPSEGKV
                     LAVGPGKKDEKGNVTPVQVTVGSVIMYKKWGGTEIKAEGKDLLLVKEEDVIAIIEG"
     gene            7073..8701
                     /gene="groL"
                     /locus_tag="P5014_00040"
     CDS             7073..8701
                     /gene="groL"
                     /locus_tag="P5014_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561045.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HRY56537.1"
                     /translation="MSAKDIIYGENAREKLKKGVEKLAKAVVTTLGPKGGNVALDKSW
                     GTPQVVNDGVTIAKEIDLEDKFENMGAQIVKEAASKTNDLAGDGTTTAVVLARAVVNE
                     GLKNISAGTNAMVLRGGLEKATKALVEEIAKFAKKISSKEEKIQVATISAQNKEVGEL
                     IAEAMEKVGEAGVITVDESKGSNIELEYKEGMQFDKGYLSPYFVTNPEKMESVLENPY
                     ILLTDAKISNMQDMLPLLESLMKVSKDLVIISDDVDGEALATLVVNKLRGVLNVLAIK
                     APGFGDNRKALLEDISILTGATLISEEMGKKISGATVEDLGRADRVISDKDSTTVVGG
                     KGNEAEIKDRVKMIQTQIENETSTYEKEKLQERLAKLSGGVAVVKVGAATEAELKELK
                     LRVEDAVNATKAAVEEGIVPGGGIAFLRARKVLKDLHFEGDEQVGVSILEKALEQPIR
                     MIVKNTGGDDGKVMAEIERRAKEENNENIGYDVLKMDYVDMVESGIIDPAKVTRSALQ
                     NAVSAATMIITTECLVTELPKKDEKAPMQMPEDY"
     gene            complement(8773..9783)
                     /locus_tag="P5014_00045"
     CDS             complement(8773..9783)
                     /locus_tag="P5014_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56538.1"
                     /translation="MKKNSINPCKISVIKTLSYSSFFGFPLSIDEIIKNLISQNNFSD
                     KLIKKNLEELVNAKIVRKTKGRYIISGVKNIDREKRLKNTSEIIEKNKSYLKILSRIP
                     WIKMIALTGSVANQNSDKKADIDLLFITESNRLWICRGLVFIVLKIVGQLPSDKSKRD
                     ICPNIFIEEGNMGWPKKKRNLYVAQNIISMKPYLWRDEIYLNFINANRWVGKYYPNFI
                     IDFSKDVKKKKLRKNFIMNFIESLAMKVQILHMKKDITTETVNKKLIHFNKNDSSKRI
                     LSGYKKIYKDTLKKLEKDLEKRDKIKNPQIEVGSKDITRKRKDTKKITKQIKQTESKG
                     EK"
     gene            complement(9790..10188)
                     /locus_tag="P5014_00050"
     CDS             complement(9790..10188)
                     /locus_tag="P5014_00050"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF024848.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HRY56539.1"
                     /translation="MKLFIHTDGGARGNPGPGAVGVVVLDEKKKVIKEIGKFIGKSTN
                     NEAEYKAVLEGLKAAKDLKGSEIVFYIDSLLVVSQLNGKFKVKEPRMKTFFNEAKMIE
                     KGFESVKYNHVPREKNYIADKIVNEVLDTI"
     gene            complement(10193..10798)
                     /locus_tag="P5014_00055"
     CDS             complement(10193..10798)
                     /locus_tag="P5014_00055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56540.1"
                     /translation="MEFREIFKKIRKNRQVLALCALIGLITGILIHFLPSNYTASGSF
                     YITRKEDNSESFFTYEGYYSQQTASTYTNSVVALAESEDVKKQVLNKLDLPEDGYNIR
                     KISKSMSVKKTGPQIINIAFKDKDLNKSKTIWETIADTLIDTSKEINRDGDGKLAVVK
                     ISEEPFVKQFYKPLFITIFAGIFVGFSLGIFIISLKEYFKD"
     assembly_gap    10989..11088
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <11089..13856
                     /locus_tag="P5014_00060"
     CDS             <11089..13856
                     /locus_tag="P5014_00060"
                     /inference="COORDINATES: protein motif:HMM:NF024594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4012 domain-containing protein"
                     /protein_id="HRY56541.1"
                     /translation="AKKSSAKFLLASTIDIYQGRLSQVDLGKYFGSSGGEENRFSLLE
                     AKRFAEAVVWEYFKRHNLDARIVRLPEVYGPRMDLSSSGFLGGYIKNVLDGSSIDIYG
                     EGEEKEFYLYISDVISGLVKAQFSEGTSGDIYSLVPENPVSSLETAYLVRSLADSKLS
                     IQFKKGLSEIREPLSIPESFNVKSIGWKPKVQLKEGVMKTLEFFDYSPNTNAFKPAKY
                     LEQKLKKVESVPESIVSLQGVKISNLPKNDESERFFNNTFKETAENQIEPNNALPEKK
                     IKNKFKFSLFNKSSKKSNIKDFSNKVRTDLGYFNAILGIVISALLVFIGVPSLSTYIN
                     IKKGYSSLNKVKQNIMSLDSANLSSDTLSAYNGFKNGKSSLKRIRWVFKIIGKDENYI
                     TYDKLLSSLMFFSKAGNLGSDALKPVESMLETLKPGSTLTLDATLFDRAKIGIVGARD
                     YIRMAEADLVGIDNSLIPEKYKNYLDEYRGVLSQSQEFADSASVVMSGLPQLLGMEGE
                     KKYIIWFQNSNEIRPTGGFIGSYATVSIDSGKIKNILIDDIYNPDGQIDVRNIKTTPP
                     DPIKNYLDEEVLYLRNSNWDPDFTKSAKVFDDIYFKITGESVDGYIAVDLKFVESLLR
                     VTGPVFLASYNEDINSENLDERAQYYSGFDYKEGSTDKKSFLTILGGKLLEKIFSLKK
                     EDFPKLVTELGRSLEERHLQISLNNNQINSLLSENKWDGSLVDTEGDYLYVVNANLGG
                     TKSNYYVENKMTYEINSMTRDGLLRANLYLDYKNNAENDAWPGGPYTDYVRVLVKDGS
                     KLTGAKIINNTGEETDIFKDVVVSKVGVYSSFETSFKLLPKESIRLVISYDLPQKASI
                     TKEGSSYSLYWQKQPGTGKDNYSFIFNPPFGSVTQNLSNNLQVDGTSIKSSGILIKDM
                     EYYISFK"
     gene            13900..15612
                     /locus_tag="P5014_00065"
     CDS             13900..15612
                     /locus_tag="P5014_00065"
                     /inference="COORDINATES: protein motif:HMM:NF024078.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PrgI family protein"
                     /protein_id="HRY56542.1"
                     /translation="MASAYMPQSNRQHPVPQNIMDVEFKLIGELTMRQFSYLLVFGLA
                     SYLSLKGIPGLFRVPTALFFGLLALGLAFVPVGERGLDEWIVNFFKSINSPTQRKWLK
                     EPQLPTAFLYDNLAIVQHEMITLAPTSSRRKLEEYLKFKSYESDEDPLDIPEKSYVMK
                     LKQIYQDTYSPELTGGVGVLVDEPEVSVGPEIPNIPGVPVIPEDAEPKYNLENEEPNK
                     ETDKLTSEYKAFSSTEDFGKEVIKEAKVSEEFKTEQTPPVPPILQKSKFVDTGVNRQR
                     MRRGSVSEKTSMRNISQPPSELDMYSYSSVTPDMHSGRKFTNLVPSEGEIVLPIRGER
                     VLSTSVEQSIDSDIQEKARKLEELLQKISQEEGVVIKKTPVQKPATSPVDRVAEEMAE
                     KLKKQNEELNVEIKRLKDQIQMGKSSSVETSVQENLLKKLESQKGDISSSYSELRKQV
                     QDLQMKLDEKVKVSTGEEFLNKVKKQLPVLTDTPNVLTGVVRDAEGKNLGDTLLIVKN
                     SRGDTIRALKTNSLGQFVVSTPLQNGTYTVEVSPVNKTNLTFAIIPIEVKGEVIPTLD
                     VIGK"
     gene            15615..16322
                     /locus_tag="P5014_00070"
     CDS             15615..16322
                     /locus_tag="P5014_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56543.1"
                     /translation="MSAQEIISTTQDHLDIFDIKDNFAVLKNGTVCAVIQTTAVNFDL
                     LSEIEQDAIIAAFSMLLNSITFPLQVVIRSRKLDISKYVEKVRKVESKIIDPLLKHQA
                     ESYRKFVQDVIKVNEVLDKKFYVVVSTTNVVVTQKGGGAFDWMLKLSGMQNKRVGVVN
                     VEKILQDGRTQLSPRIDHVIREFGRIGIKCKQLTTQELVELYFDIYNPSNIHGQRIRT
                     NLDDYKTAIVNPAIIEE"
     gene            16326..18872
                     /locus_tag="P5014_00075"
     CDS             16326..18872
                     /locus_tag="P5014_00075"
                     /inference="COORDINATES: protein motif:HMM:NF040179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRY56544.1"
                     /translation="MALNIFDLFKKSTKKTVSNTGDTAEVEKTPPEILAEGMMNVRDI
                     IAPSAIEVDFNDIRIGDMYYRTFFVSGYPRFVGANWLSPVINFDHTLLISMFYYPVKS
                     KVILDDLRKKITELEATTMTNREKGKISDPVVEAALEDATALQEQLVKGVEKYFQFSF
                     YITIPAYEKEELDNVSSKLESLLASLLLISKTSALQMEDAFRSCIPTGIDRLMVTRNM
                     DTTSLATTFPFTSSDLTMEEGIMYGINKHNGSLVIFDRFSMENANSVVFAKSGAGKSY
                     FVKLEALRHLVFGAHVMIIDPEEEYYNLCEVVGGNYIGFSANSKHKINPFDLSGIATE
                     GENELGQKLISLVTLLKLMMGQLNSTEEAILDRALIETYRMKGITTDPDTQSSKEPPV
                     MEDLYKVLLGGSEPESKTMADRLEKFIKGSMAGIFDSQSNINLESSMTVFSTKNLEDA
                     LRPIAFYMILDFVWTTIRRDLRKRILIVEEAWYLMQNPDSARFIYGIAKRARKYYLGL
                     TTISQDVDDFLTSEYGKAVVTNSSIQMLFKQHPAAIDKVVETFYLSEGEKRFLLSAGV
                     GEGLFFAGSNHVAIKVMASESEHKLITTNPEEVLKLKEERRLEEKKGQLDLARKEELR
                     MEYKPYKAPEPMDKYTTFDELGETKQEFKPIKNYENENINSEVNKTVPIETKPVLEPK
                     SDLTIKSRIVDKVVPEPQKTVQKPMNETNLQKDQKPIVIDLNKKNQTFDAGITNNPAP
                     TFKSAPAPENVSVSMPTPAPTLTPSPVPTPTQVINQNNQQSQPPAQKPKNILDNKVIE
                     LRKKMLQDEDSFLDNAPSANEIMDSVEEMKRNKPMINNQPPIQNNPTIQQ"
     gene            complement(18876..19982)
                     /gene="dnaN"
                     /locus_tag="P5014_00080"
     CDS             complement(18876..19982)
                     /gene="dnaN"
                     /locus_tag="P5014_00080"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HRY56545.1"
                     /translation="MKFSFLQENLSKGLQTIMHAIPAKSSLPILSNVLIETDKGRIKL
                     SATNLETAITTYVGASIEEEGSITVPARLLKDFISNLSPGTINATVKDEILVVNSKST
                     KSKFNGSSAKDFPELPSFPKNAKGLSIDPGLLNSVVGVVAFASGVDTSRPIFTGIYMD
                     YSKEKLTITSTDGFRLSEKNIKIKGEISDFKVVIPAKTLLEVAKIFSTSSEPIEFTLN
                     QDENLGLFRAEDTLIATRVLDGDYPDYKKIIPTEHVVKANFSTLEFTEAVRLTDIFAK
                     EGNSTIKVRFDPEGKIKITSLSEETGQHESNLNAEVEGELVEIAFNSKYLLDFLGNVK
                     SENIEFKSNGNLSPCTFVPDGIKDFIHIIMPMQI"
     gene            complement(19992..20435)
                     /locus_tag="P5014_00085"
     CDS             complement(19992..20435)
                     /locus_tag="P5014_00085"
                     /inference="COORDINATES: protein motif:HMM:NF013583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HRY56546.1"
                     /translation="MNKIFQYIGIKPELSVEEIEENNFNVTVSGDDLNFLIGFRGQSL
                     DGLQNILRLMIYRETQIQPLITLDINDYKSRKTEKIQDMAKTFIDKVRFFQKDVEMPR
                     MSPWERRQIHVLVSEYDDIESESTGEGEDRRVVLKPKKLNKGKKE"
     gene            complement(20500..21276)
                     /locus_tag="P5014_00090"
     CDS             complement(20500..21276)
                     /locus_tag="P5014_00090"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HRY56547.1"
                     /translation="MWNTILINPIFNLLIVLYRIFGNLGVSIILFTIIAKAALIPATM
                     PSIKMAKKQRDIQPELEKIKQKFKYDKKKQAELQMDLFKKHGINPGAGCITTLITFVL
                     MIAIYRSLTTLTTASDLVALNSRIYIDSFKFAHDEALATKFLHLNLTKPDPYLILTIL
                     TVVAQFVATKMMSPFTKVSNKAVKQTPGQADDMMQAMQKQNQYIMPLMFFIFGITLPS
                     GVMLYIFISTVFQIGQTYFFSGWGGLKPFINKLKSGKKSS"
     gene            complement(21300..21515)
                     /gene="yidD"
                     /locus_tag="P5014_00095"
     CDS             complement(21300..21515)
                     /gene="yidD"
                     /locus_tag="P5014_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017752000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HRY56548.1"
                     /translation="MKKLALNLIKFYKKHLSLDNKGFKVCRYTPTCSEYTYQAIEKYG
                     VLKGSLMGFGRILRCNPFSKGGHDPVK"
     gene            complement(21460..21807)
                     /gene="rnpA"
                     /locus_tag="P5014_00100"
     CDS             complement(21460..21807)
                     /gene="rnpA"
                     /locus_tag="P5014_00100"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HRY56549.1"
                     /translation="MLKKENRLSSNFEFNVTRKYGKYYKGKYFHIYFLKPKNYEGPTK
                     AGVVVSNKFDKRAVVRNRIKRIFRDIIRKNFESVSDKGLWVVIHPKTACTNVEYEEIS
                     SEFNKILQEASFS"
     gene            complement(21828..22001)
                     /gene="rpmH"
                     /locus_tag="P5014_00105"
     CDS             complement(21828..22001)
                     /gene="rpmH"
                     /locus_tag="P5014_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018944472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HRY56550.1"
                     /translation="MPKTKRTWQPKKRKRIKKHGFMERMSTPGGKNVIKRRMAKGRKK
                     LVVSKRSKVDHKR"
     gene            22061..22402
                     /gene="metG"
                     /locus_tag="P5014_00110"
     CDS             22061..22402
                     /gene="metG"
                     /locus_tag="P5014_00110"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase subunit beta"
                     /protein_id="HRY56551.1"
                     /translation="MNEQKPLIKFDDFSKVDMRIGYVESAEEIEGSDKLIKMQVDFGD
                     MGKRQILAGIKSNYTPADLVGKKLPFVVNIEPRKIMGNESQGMILAADFEDKPVIFHL
                     EKDVSNGTLIR"
     gene            22408..23673
                     /locus_tag="P5014_00115"
     CDS             22408..23673
                     /locus_tag="P5014_00115"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HRY56552.1"
                     /translation="MVLRLRKSKKYYTKTFGCYANEVDSDYIAGLLEDLGFSELKKKG
                     RNETEEIEYALKNADILVINSCSVRQKSEDKVYGIGKMIKNNPKARAKVVLTGCMVGS
                     VTGDRKRFSEKQLKMKTKDFDGYFPPSDIKSFLTVLRELNLISKDLFFDKKGLKRRQR
                     DKNHAYVNVSYGCDNFCSYCVVPYSRGCEVSRSKKEILEEIKCLVEKGVCDFTFCGQN
                     VNSWGLSMEEKFKVRSAGRKDTDNKKQSKLPFAQLLREIHNIKEINNIDFISSNPFDF
                     TQDLVDVLKLPKISNYVHIAVQSGNNKILKAMNRRHTSEEFVNLVERIRKVRPGIEIG
                     TDIIVGFPGETREQFMDTAKLIEKVKFSVAFISMYSSRKGTFAEINLKDDVPLKEKKW
                     RHMYLTKEWRKNKPKVKSNYSHSRWVSSE"
     gene            23680..24615
                     /gene="miaA"
                     /locus_tag="P5014_00120"
     CDS             23680..24615
                     /gene="miaA"
                     /locus_tag="P5014_00120"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: protein motif:HMM:TIGR00174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HRY56553.1"
                     /translation="MSKNSKIFIILGPTSSGKTSIALDLCKIFDGEIISADSRQVCKF
                     MDIGTGKVPIKSDINLKKFKDRWELNDVKVWGYDLVSPDDFFSGYDFADYSLKKAKEI
                     LEKGKIVFLVGGTGFYVDLFTGKMKPSKVLPDLNLRDSLEKLSLSDLQNKLKALNEKA
                     FQNIDQKNKVRLIRAIEKELSIDVRNEQLPYLDDAKFTYIGLTGDRDYLYKRVDNWVD
                     EIWDGGLIDEVKSLVEMGYGDSSKIKGIVYKEVFDYIEGNLKKSEAIQKTKFNLHSYI
                     RRQQTWFKKNNLIEWFDITKDDFKEIIYNRIKENL"
     gene            24616..25605
                     /locus_tag="P5014_00125"
     CDS             24616..25605
                     /locus_tag="P5014_00125"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HRY56554.1"
                     /translation="MDKQIVISIKTIILFLLTILGLFVIYRLGPIIGILLLSTLLVIS
                     MEQPVRYFMRGFLFNKRISRGLAVLISYSLFVLIILGVLTFVVPPLITELQKMIVSIP
                     YMLQNVKIPQEWQSSVPNMIPDTSKISTGVINATFSIFSNFATFLSVLVISIYMSLDW
                     ENIKKGFISLFPKNNEDIVQETIEDIEKNVGAWIKGQLLLMLVIGVASFIGLSILGVR
                     YSVGLGLLSGIFEAIPNVGPVLSAIVAGLIGFTDSPIKGIGVVSLFVIIQQLENNLLV
                     PKIMGKVSGFRPLVILLALLVGANFFGIIGAVCAVPVTMIASTILKKFFGTNN"
     gene            26041..26391
                     /locus_tag="P5014_00130"
     CDS             26041..26391
                     /locus_tag="P5014_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56555.1"
                     /translation="MGNLLFKRKKRNQENVFRPTEELILEKRLQEALALKGYKLGKEH
                     QDSFQIITYSIVKPGFLSFLSIGSIIIREQLQEVELWVYRLGNYWVDDLMDILSAFEK
                     EVGHSIQKEVVTSF"
     gene            26468..27550
                     /gene="mltG"
                     /locus_tag="P5014_00135"
     CDS             26468..27550
                     /gene="mltG"
                     /locus_tag="P5014_00135"
                     /inference="COORDINATES: protein motif:HMM:NF014656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HRY56556.1"
                     /translation="MSEELDPEKYHVLTPKKKKLVVFFLLFLFLFFIPLMSLIYYKSA
                     VHRPSQTAKEANFEIGSGDDVFEIAQMLYEKEAINSEFLFIFYVFLNRSDTNIQAGTY
                     KIPAGTSLVTLVDMLKNGRNDISLTFLEGWRLEQFAIYASRKLKNFNYQDFMEIAKPY
                     EGYLFPDTYFFNLDADEEEVFNKLRDTFDKKTTNILTGENLDKVGLSKQEAVIFASMV
                     EREVSNPEDAPIVAGILIKRWKEGMKLDVDATTQYSVAYKRLCGGDGGDIICTPTLED
                     MYDIDWWPHNLTREELDTKNPYNTRAVVGLPPTPISSFSLSSLSAVINYEKTPYYYYL
                     TDKDGIMRYSVSLSEHEQNISNYLSN"
     gene            complement(27557..27958)
                     /locus_tag="P5014_00140"
     CDS             complement(27557..27958)
                     /locus_tag="P5014_00140"
                     /inference="COORDINATES: protein motif:HMM:NF014618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF151 domain-containing protein"
                     /protein_id="HRY56557.1"
                     /translation="MYKPVKFITEVPYTDFSTLYLFCKSLKTLLPIEIIFNNKTTTPS
                     IYNSIKRILYGCGIDIAHIKIYHCYCNNFYAYLTLKKEGRDFDINIGIKDALEISKEA
                     AIPIFVKDKILENYGIKITKDLIIKSLKDSD"
     gene            27993..28427
                     /locus_tag="P5014_00145"
     CDS             27993..28427
                     /locus_tag="P5014_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56558.1"
                     /translation="MSNLLKNTYDKMEAIKGKRLVYMLLAIFVSFTTVGISIGYLMSP
                     KLNEDETPVKTYSGNLPKSEKTEASGKITYVNPEMYPIDEISYSLTDGSGKDIYLLSA
                     KDNKLEISEGLYVKVIGTLSTLKDGKTPVLLVEEVIINNVSN"
     gene            28414..29100
                     /gene="ftsE"
                     /locus_tag="P5014_00150"
     CDS             28414..29100
                     /gene="ftsE"
                     /locus_tag="P5014_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011879364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HRY56559.1"
                     /translation="MSQIEFKNVTKEYIPGNKVLDDVSFSIEKGEFIFIIGPSGAGKS
                     TIIKLLTREEKPEEGQVVFNNQDISNIKPEEMPEFRRKIGVVYQDYKLLNSKTVYDNV
                     AVSLEVVDADEQEIKSVVPNVLSLVGLLDHSSYYPQELSGGEKQRVAIARALAHEPDV
                     LIADEATGMIDPDVSDEIVELLEKINSLGTTVIMATHDQRIVDKLTKRVIKIQKGKLV
                     SDKKGGKYRA"
     gene            29144..29980
                     /locus_tag="P5014_00155"
     CDS             29144..29980
                     /locus_tag="P5014_00155"
                     /inference="COORDINATES: protein motif:HMM:NF037183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease-like cell division protein FtsX"
                     /protein_id="HRY56560.1"
                     /translation="MTVSNILVMGVTFLLLGIFIEVVVLSQTMLKYLEDQAQITVFFK
                     DEFGEDKIIPFKDSLLSDDRISEVTYISKEEALKIFKDINQDQPVLLESISASVLPAS
                     LEIKTKKITDLEGLTNELQSKEGVEEVRFYKDVIQKFKSVSNAVYIVGFILVIMFFLI
                     SYSVIISSLRTAIDSRGQELEVMRLVGAKDSYMTKPFMYQGVFFSAVSSFLSSIVIFI
                     LGFVESKLGVFSRGLSFGFLPKVFMNPVIFSLILAFILILSGFVLGYFGSKAAVKKYL
                     KY"
     gene            29988..31238
                     /locus_tag="P5014_00160"
     CDS             29988..31238
                     /locus_tag="P5014_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56561.1"
                     /translation="MKINKHFFLVIAVIFAFLLPFTIPSFAQEETSNEDEEEIQDIQE
                     KIEKYEKKISDLRGKANTLQNEIDYMDSQIGLTQLKIQNSIANIVKTEDRIKELSEGI
                     ENLGIRIDKLVKSIEYQEMILGSRMRERYKDRGNSMLMLLFGSNTLNSLVKKTEYLRA
                     LEENDNKLIDQMAETRTSFEQQKDIFQNKKTEQEELKNKLQIEKANLDSYQRSLDSQK
                     EAKEKLLADTQNDEAKYQELLAEAEKELASFAGFVQSAGGGIIGSNEFGSGKEGWYLS
                     QRDARWANQKIGNSNYTVFEVGCLITSVAMYNRYYGNNISPGDVAKDDSRFFSNTAMM
                     LIPWKAPPGKSYKAITASSISDELEKRPVIVGVYAGPYGTHFVVLSKKSGSDYIMYDP
                     WYGPDLKFSDYYQKSSIYSAVVFK"
     gene            31312..31989
                     /locus_tag="P5014_00165"
     CDS             31312..31989
                     /locus_tag="P5014_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56562.1"
                     /translation="MIFIALIVFYRILCIAENNYFDFSLNRYLIDENKETVVNFKYKL
                     EGYDEKLFLVKPFIENGIVEVFNAEKNLWVSSFSLVSDLPSLQKEMLIRIKGFGIEKA
                     NLYFEILNLSNGQVYLTPKKYVWSKKVYEKYLEDLNKKIKGDLVLKNEGEGVQEETLD
                     SVFLKDSSRESIYKKLYEEITEDCFYLLVFVEFLIFFYLGFRKSKNSVKRIDIESRTY
                     GVNGKIP"
     gene            31989..32360
                     /locus_tag="P5014_00170"
     CDS             31989..32360
                     /locus_tag="P5014_00170"
                     /inference="COORDINATES: protein motif:HMM:NF037970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HRY56563.1"
                     /translation="MKKIINWLPALVVMTIIFILSSQPGEVVNSTVANTESVQKIGHV
                     IMSMILCVSYFKATKNIPLSILLSLLYAIFDEYHQSFTADRSSSISDVFIDVLGASVS
                     GIFLWKVLPNLPKKLKELLLN"
     gene            32306..32716
                     /gene="tsaE"
                     /locus_tag="P5014_00175"
     CDS             32306..32716
                     /gene="tsaE"
                     /locus_tag="P5014_00175"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HRY56564.1"
                     /translation="MESITKSTEETKRIAIELAGKVHKGSVLALYGNLGSGKTTFTRY
                     FVEALGLNNRVQSPTFVVARKYGYVNHVDLYRLTSENEAKDIGIEEMLTDSDSLTLIE
                     WPEIIENILPKNSVKIYFEYIDENTRKITIENLH"
     gene            32700..33044
                     /locus_tag="P5014_00180"
     CDS             32700..33044
                     /locus_tag="P5014_00180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56565.1"
                     /translation="MKIYIDSSQRYMKKVSLLECVGDTEKKLDLVEGSIDVVGAIRDL
                     LKRNKLELKDISEFEPMLGPGSFTGLKVGVTVSNMFNWLLGRKDLKNLDLPEYGGEPN
                     ISEPKRKEPIGR"
     gene            complement(33061..33366)
                     /locus_tag="P5014_00185"
     CDS             complement(33061..33366)
                     /locus_tag="P5014_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56566.1"
                     /translation="MTERYSNQRSFEDIKKFLGEIKTRVDGIEENETLQKINLEESIK
                     LLVEEIIELLYNEGEIEANNFYALPWKIRKGIKNSLRNAFGIDFFEDENSNTLFKKQ"
     gene            complement(33369..33677)
                     /locus_tag="P5014_00190"
     CDS             complement(33369..33677)
                     /locus_tag="P5014_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56567.1"
                     /translation="MARENNSASTAEVRRAKKLEQEVSSIIERTKQREGKLGYYNLYN
                     EQMRSLTEVLLLLWSLGLIKDYTYPTLSWKSKYRVNIFMKLILSFLEADEENNTKEKR
                     "
     gene            33760..>34657
                     /locus_tag="P5014_00195"
     CDS             33760..>34657
                     /locus_tag="P5014_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015023165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRY56568.1"
                     /translation="MKILVTGAAGFIGSHLSERLVDLGHEVVGIDNFNPYYSVELKRL
                     NASDLEKKGVKIHEFDISEDVIDPIFDGVEAVFHFAAQPGISASTPFSTFMKNNLVAT
                     ENMYQATKDLPNFKIFVNISTSSVYGKNATDDENTPPKPTSHYGVTKLAAEQLLLAYQ
                     RDVGFPACSTRLFSIYGERERPDKLYPKVIDSILNDRPFPFYEGSEKHLRSFTYVGDI
                     VDGFVAILNNYEKCLGEIFNLGFDGAITVGEGLKIVEDYLGKKAIYDIRPPRLGDQLE
                     TRATIKKAREVLGYNPTTTPEEG"
BASE COUNT        11434 a         5301 c         6416 g        11406 t
ORIGIN      
        1 aaatatctcc tttattagcg ataattattt ctttagcttt aatttctttc tcaataatat
       61 attccatcat ttacctggat gattatttcg tagctatgct ttttatctat atagccatgc
      121 aatttgctta ctctttttat cttagaaatg taatcatcct tgactcttta attgtagctt
      181 ccggattcat aattagggtg ttttcgggag gttttgtttc cagaacctca atttcatctt
      241 ggttaatact tacaaccata ggcatatcta tgttgttagc ctttggaaaa aggagatctg
      301 aaaaaaccat tttagcaaaa tattctgtgg caaacgatga tataaaaacc agagaaacct
      361 tgagacgtta tcctgatacc ttgcttgata gtatgatatc aatgtccgca tcttatacta
      421 tacttacata ttcacttttt acttttatgg tttctccggg tgaggtaggg gatagaatat
      481 ccagttttct acccacagtg ttaaagagcc cgaaattgat gatgcttaca attcctttgg
      541 ttatttacgg aatagcaagg tatctttatg taatttatga aaaagaggag ggagaatctc
      601 ctgagagagt tttattatcg gacaagccct tactacttac agtattaatc tggggtttgg
      661 tagcatcagt gataatattt tacttgccga ttcttagtta gtttttttct gatacattag
      721 aattttcttc tactgtttct tctttattct ctgtagtttg ttcatttaat ttttcaattt
      781 catctgtcct attaatatcc ccaacaaatt tatcttgttg ttcttgataa tattctcttt
      841 ccatttttaa tatcatttct ttaacatcta aaggaacttc agggttatca attgatttta
      901 ttgaggattc ttcataaatt gaatcttgtg cggaaagaac atttgaattt tcctcaaagc
      961 tcattaatcc cataaaccca gctggaagta caagcattcc taaaaacatt gctaataaaa
     1021 ccgtattaaa accaatttta aatactgttc tcattaaaga tctcccggta attcaattaa
     1081 acttttatct gtatcatatt caacatcttt atcctttttt atcttcattt cctcttctac
     1141 gtcaaataca aacaataatg ttgttaatag agtaacgcca gtaattatgt agaacaccca
     1201 tttatacata agattggaca tggccattat ccctgaaact aaaagaagca tatatgagta
     1261 ggacacccag accgctccct gagtatatat ttcatatgaa ttttgaatta acgattccgc
     1321 tttaattgag aatagcttgg ctgttatggt tggttttatg tgagaatcgt ggaagaacaa
     1381 tgattttatt aatacataaa ataatcctat aaccaacata gtggtcataa ataataccga
     1441 ataggagttt acctttatgt aatcatagga attttggaca ataagtccgc gatcggttat
     1501 tttaaaagct actcctaaat atttggacac aagtactatt gaaatcagtc tggatgctat
     1561 aagcaacact gctggaatta tggctttatc tattaatctt gctagtattt tggacagggt
     1621 cattttaatt taccctaaac atgatttttc aagcaaacat tgatcagggc tgatattata
     1681 ttactactat gagaacaata cagtcaattg aggatattct aaaaaagcca tattttttgc
     1741 ttttttcaat tttattgttt tttacaccct tagtttttag tcagaatacc aacgagctgt
     1801 ttgagtttcc aaaaatgttc tttgtttttg ttatagggct tttcataatt ctcttttttg
     1861 cgacagacac aataataaaa tccaaaaaaa ttatcagtcc cggtgttttg gttgttttat
     1921 ttttgttatt aacttccatc tcaacatttt tttctactca caaatacaca tctttggttg
     1981 ggtattactc aagatttaac gattctttat tttcatattt tatatacttt tttctttatc
     2041 ttatatgtat aaacagaatt accaaagaag attacgaaaa gttaataaaa ttagttttga
     2101 tatcaataat tccagttagt ctctacggaa ttgcccaaag catcagtggt gtaaccagag
     2161 tctactccac cttcggtcaa cctaattggt tatcgcagta tttaggaatg ctactcccaa
     2221 tgactgctta ctattttttt actgaaaagg gtgaaagatt taaattgtgg ggattggtgt
     2281 atatttttgg cttctattgt ttatgggtaa ccttttcagt ttcaggttta ttaggtttct
     2341 ttgctggtgt attctatctg attgtaaaat attttagaaa aatttttgaa aataaagtat
     2401 ataaaacgaa gttgattttt ctcatttcga taacagcctt gatagcgttg cttaatatcg
     2461 ggttatataa agaaaaattt tatgattttt atttagatgt tcagaagcaa tttgttttaa
     2521 taaaaaaggc ctatgcttca gaaaatatta ataggttatc agacccgggg tttattagat
     2581 tagagttatg gaaaagtaca ttcgatttaa taacttcttc acccaagaca tttctatttg
     2641 gtacgggacc ggaaacattt ccttatgtat ttcaaaatta tcgtaacggg aaactaaatt
     2701 attcttcgga atgggatttt gtttttaata aaccccacaa ttattattta gagatttggt
     2761 ctgaatccgg aattttaacc ttgggaattt ttattgcctt aatttacaca tatgttaaaa
     2821 aattacctaa tcacatggct tcggcaattg tagttttttc tgtaacaaat atttttgggt
     2881 ggccggttgt tcctacaagt ttactgtttt ggttatttct tagcggatta gaaattaatg
     2941 atgttaaatt aaaacctttt gaaactgaga cagctaacaa attaaaactc aataaagtac
     3001 ggttacttta taagacttta ttaataacac tgatcgttgg tgtttattta tatttaaatt
     3061 tttatatttt caaattttat aatgccgata tttattataa aaaaggacag gaatatatta
     3121 aaaaaggtga agagaaaaaa gctttatatt ttgcagacaa ggcagtgata gctaacccac
     3181 ttgagccaaa ttattatcgt gggagagcga aagtgaatac agttttacta gtctcatcac
     3241 aggataaaca aaaaataaaa caggaaatat ttgaagatct tacaaaatca tttgaactaa
     3301 ataaaaataa tttagtcacc attagaaatt ccattccaat atattatttt ctggctgtgg
     3361 gagatattta taaagctatc agcaataata attacgacga aagatatata ggttatgtta
     3421 aagagtttta taatgaaaca aaaaataact atccaaatga tgcgggggtg atattaggtg
     3481 tagcaaagta tgaaaaaaaa ctaggtttgg aggagtcata ccaaaatagt gtagaaagaa
     3541 taaaatttct tagaccagac ttattggatt ggaacgagct attcagataa ttcgatataa
     3601 gcgtcggaga cccaaccttc aactgtttcg gaaactttta tcttaatcca acctgtttct
     3661 ttatccagaa catcataact ttctccggta ttcacctttg ttatttcagc tccgttgagg
     3721 ctcggtgttt ctcttactct taaccaacct aatggggtag gtttaacctt ggcaatttcc
     3781 ttttttacag tttcaactcc aactgacgat aaactttcca aagcgctctt cgcctcttgc
     3841 gaaattcccc cgcccgagct ttttaaccct aaatacgcac ctctttttag atccttaagt
     3901 tttgcaaaat cctcttgttt tgtaatcggg ttgccgtttg catcaaataa tccgccttta
     3961 taatcaataa aataatcaaa taacttatct ttattaattc ctacattgtc tatgtttaca
     4021 ccccttgtag tattccagta aactacccct tttgcccacg aaacggtatc agaagtaatg
     4081 tccggattat cagaggatat gttgtaaaga tttggagagt ctttaaacat ttctactatt
     4141 gggtcgacag ggaaggggaa aagtttaacc gaaacgttta aggtataacc tctctgtata
     4201 tttatgtttg tttcatttga ttcatacccg gcaagcgcga cttttaagtt atagtcaccg
     4261 cttgttatag ctgcggaaga aaaaggagta gttcctatct ttgacccatc aacatagaca
     4321 tcagctccgg aaggttcaga aacaattcta agggtattat cagaaccgtc tttatcaaac
     4381 caaagttctc ttcctgatga gaataaatct gatattccca aatctctttc tattcccaca
     4441 gtatatatgg tgtctttttt ggaaggtaaa aattttattt cagtctgata ttgtcttgtg
     4501 tcgcttctaa ttgctattac atttgttccg ggctttatgt ccttagattc aaaaggagtg
     4561 tttcctacaa cctcgttatt tataagtact tgggcctcac cgcttgctac tcttacttct
     4621 aacgcagatc tatttcctat tccggtaaga tttctaatta attcaccacc aaaaaatact
     4681 actaaagcca cacctacaat tcctaaaaaa tacagtaggg cggttgaaag tggtttctcg
     4741 tcattgctta ttgtagatac tgccatatgt acctaaatta tcacgatggt ggtaaaatat
     4801 caacatcgag atttcctttt aaaaggctta aaaatgacta caaatgaact aaaagaaaaa
     4861 tttataaagt tcttccaaga gagggggcat tcattaattc cctccgcttc tcttattcct
     4921 gaaaacgatc cgacggttct ttttaccaca gccggtatgc acccgttagt tccatatctt
     4981 ttaggagaga aacacccttt aggaaataga cttgttaacg ttcaaaaatg tttaagaacc
     5041 gacgatattg atgaggttgg ggatgatact cacgttacct tttttgaaat gcttggtaat
     5101 tggtctttag gtgattattg gaagaaagaa tctatctcat ggagtttcga attcttaaca
     5161 aaggaactta gaatacctgt tgaaaagctc gcggtgacct gttttgcagg agatgataat
     5221 gcgccaaggg acgaagagtc tgcagaaatt tggaaatctg ttggtatacc agaaaataga
     5281 atattctttc ttgggaaaat agataattgg tgggggccgg taggtagttc ggggccttgt
     5341 ggtcctgata cagaaatttt ttattggact gggggaggag aacctaataa tttagattca
     5401 gctgagaact gcaatgactg ggtggagatt tggaacaatg ttttcatgca atatgaaaaa
     5461 acatccgaag gaaaatatat cccgctaaaa caaaaaaatg ttgataccgg aatgggtgtg
     5521 gaaagaacca ttgcagttct aaatggtaaa aaaagtgttt acgaaacaga tgcttttttg
     5581 gaaatttatg aaaaagtcag tgaactatca tcttctgatg atgagatttc aaaaagaata
     5641 atttgtgacc atgttagagc ttctacattt ttattaggag atcagcgatg tattacacct
     5701 tcaaatgttg atcagggata tgttttaaga aagcttataa gaagatcgat aagaaaagca
     5761 aaaaagatag gaattaacga accttttctt gtatctttgt caaaaatcat aattgatcaa
     5821 tattccaatg attattccga gcttacagaa aatcagaatt ttatagaaaa atacttaggt
     5881 ctcgaggaag aaaagtttaa taaaatattg aacgatggtc agcaagaatc attcagagaa
     5941 attgaaaaaa tatcggatgt aaatgagatt gttaatgtag caggtattga agttttaaga
     6001 gctgcaaaaa tatcatttga tctgtatcaa tcccaaggtt atccaatgga gatgtttgtt
     6061 gaagatatga aagaaaaaaa gaacattgaa ggaagtttgt ccgagaaaat ttgtgaagac
     6121 gttgggagac taatttcaac ccaccaaaat gtttccagaa agggtgctga aaaaaagttt
     6181 aagggaggtc tttccgacaa cagtgacgat gttgtcagat atcacactgc tacccacctt
     6241 ttgaataaag cattgagaga ggttttggga gatcacgtta aacaaattgg aagtaacatc
     6301 actcccgaaa gattaaggtt tgactttcca aacaattcaa aattaacaca agaagaaatc
     6361 gaaagagttc aaaatattgt gaatgatgta gtagataaag aacttcctgt aaattttctt
     6421 gtttgctcgc acgaagatgc cgtaagttgc ggagcaactc atcttgaagg tgaaaaatat
     6481 cccgatcaag ttaaggttta ttttatcggt gatagtttag aatctagttt tagcaaagag
     6541 ttttgtggag gtccccatgt taaaaacaca aaggatatcg gccacataga aatttataaa
     6601 caagataaaa taggcgaggg aaaaatgaga atttacgcaa ggtttaagag ttaattgcag
     6661 gggttgaatt aacccactcg aaagtgttat atatttgttt gatttactaa cttacgtaat
     6721 tcatatttaa aacaggaggt attttttatg accagtatag aaaaaataaa gcctttatca
     6781 gattatttac ttatagagcc tctcgaaaaa gagacagttc tcccatccgg aattgttgtt
     6841 cctgataccg ctaaagacaa accttccgaa ggtaaggttt tggccgtagg tccgggaaaa
     6901 aaagacgaaa aagggaatgt tacgccggta caagttacag ttggttctgt catcatgtat
     6961 aaaaaatggg gcggtactga aataaaagcc gagggaaaag atcttctatt agtaaaagaa
     7021 gaggatgtta tagcaataat tgaaggttaa ttaatttaaa ggagaaatta ttatgtcagc
     7081 aaaagatatt atttacggag agaatgctag agaaaaatta aaaaaaggtg ttgaaaaact
     7141 tgcaaaagct gtagtgacaa ccttgggtcc gaaaggtggt aatgtagccc tagataaaag
     7201 ctgggggaca cctcaagttg taaatgacgg agtgacaatt gccaaagaaa ttgatctaga
     7261 agataaattt gaaaatatgg gggcacagat tgtaaaggaa gctgcatcta aaactaatga
     7321 tttagcagga gatggaacca caacagccgt agtgcttgcc agagccgttg ttaatgaagg
     7381 tttgaaaaat atttcagcag gtactaatgc tatggtatta agaggtggtt tggaaaaagc
     7441 taccaaggct ttagtagaag aaattgctaa atttgccaaa aagatttcca gcaaagaaga
     7501 aaaaatccaa gttgctacta tttcagccca aaacaaagaa gttggagaat tgattgccga
     7561 agcaatggaa aaagtcggtg aagcaggagt tattacggta gatgaatcaa aaggttccaa
     7621 catcgaattg gaatacaaag aaggaatgca gttcgataaa ggatatcttt caccctattt
     7681 tgttactaat cctgaaaaaa tggaatctgt tttggaaaat ccttacattt tattaactga
     7741 tgccaaaatt tccaatatgc aggatatgct gcctttgtta gaaagtttga tgaaggtgtc
     7801 taaagatttg gtaatcatat ctgatgatgt tgatggggaa gctttagcta cattggtggt
     7861 caataaatta agaggtgtat taaatgtttt ggctattaaa gcacccggtt ttggagataa
     7921 cagaaaagct ttacttgagg atatttcaat tcttaccgga gctactttga taagcgaaga
     7981 aatgggcaaa aagattagtg gagctactgt agaagatttg ggaagagctg accgagttat
     8041 atcagacaag gatagcacca ctgtagttgg aggtaagggc aacgaagcag aaataaaaga
     8101 tagagtaaag atgattcaaa cccaaattga aaatgaaaca tcaacttacg aaaaagaaaa
     8161 acttcaggaa agattagcta aacttagtgg cggggtggca gtagttaagg tcggagccgc
     8221 tacagaagct gagttaaaag aactaaagct aagggttgag gatgcagtta atgctaccaa
     8281 agcagctgtg gaagagggta tagtgcccgg aggcggaata gcctttttaa gagcaagaaa
     8341 agtcttaaaa gatttgcatt ttgaaggtga cgagcaagtg ggagtaagta ttcttgaaaa
     8401 agcattggag caaccaataa gaatgattgt taaaaatact ggaggagatg atggaaaagt
     8461 catggctgaa attgaaagaa gggcaaaaga agaaaacaat gaaaacattg gttacgacgt
     8521 gttaaaaatg gattatgtcg atatggttga atcaggaatt attgatcccg ctaaggtcac
     8581 cagaagtgcc ttgcagaatg cagtatctgc ggcaactatg ataataacaa cagaatgcct
     8641 ggttacagag ttacctaaaa aagatgaaaa agctccgatg caaatgcccg aagattacta
     8701 aaaattttgt aattaccaaa aaggcaaaaa aaggggaggg ggcgaagccc cctccccttt
     8761 tttcccattt atttattttt ccccctttga ttctgtttgt ttaatttgtt ttgttatttt
     8821 tttggtatct ttcctttttc tggtaatatc tttacttcca acttcaattt gcggattttt
     8881 gattttatct cttttttcca aatccttctc taattttttt aatgtgtctt tgtatatttt
     8941 tttatatcca gacaaaatcc tcttacttga atcattttta ttaaaatgta ttaacttctt
     9001 gtttacagtt tcagtggtaa tatctttttt catgtgcagt atttgcactt tcattgccaa
     9061 agattcgatg aaattcataa taaaattttt ccttaacttt ttctttttaa catcttttga
     9121 aaaatcgatt ataaaatttg ggtaatattt acctacccac ctatttgcat tgatgaaatt
     9181 taaatatatc tcgtctctcc ataaataagg tttcatcgat attatgtttt gagctacata
     9241 aagatttctt ttctttttag gccagcccat attaccttct tctatgaaaa tgttggggca
     9301 gatatctctt ttactcttgt ctgaaggaag ctgcccaact attttaagaa ctataaaaac
     9361 caaccctcta catatccata atctattact ttcggtaata aacaacaaat ctatgtctgc
     9421 ctttttatca ctgttttggt tagcaacaga tcctgtcaga gcaatcattt ttatccatgg
     9481 gattctagac aatattttca aataagattt atttttttca ataatttcag aagtattttt
     9541 taaccttttt tctcgatcta tatttttaac tccggaaatt atgtatcttc cttttgtttt
     9601 tcttacaatc tttgcattta caagttcttc taagtttttt ttaattaatt tatctgaaaa
     9661 attattttgc gaaattaaat ttttgataat ctcatcgata cttaaaggaa atccgaaaaa
     9721 actactgtac gagagagttt taattacgga aatcttacaa ggattaatgc tgtttttctt
     9781 cattttaatt taaattgtat caagtacttc gttcactatt ttgtcggcta tataattctt
     9841 ctctctaggt acgtggttat atttaactga ttcaaaacct ttctcaatca tttttgcttc
     9901 gttaaagaaa gttttcattc gtggttcttt tactttgaat tttccattaa gctggctgac
     9961 cacgagcagg ctatctatat aaaaaacaat ctcacttcct tttaaatctt ttgccgcttt
    10021 caacccttct aaaacagctt tatattcagc ctcgttattt gttgatttac caataaattt
    10081 cccaatctct ttaataactt tctttttttc atccaacact actactccta ccgctcctgg
    10141 cccgggatta cctctcgctc ccccgtctgt gtgaataaat aatttcatat aattagtcct
    10201 taaaatattc tttgaggctg attatgaaaa tacccaaaga aaaaccaaca aaaattcccg
    10261 caaaaattgt aataaagaga ggtttgtaaa attgtttaac gaaaggttct tcagagattt
    10321 ttacgacagc aagtttccca tctccatcac gatttatttc cttagaagta tcaattagtg
    10381 tgtctgctat agtttcccaa attgttttag acttgtttaa atccttgtct ttgaaagcaa
    10441 tattgattat ttgaggaccg gtttttttta cagacatcga tttactaatt tttcttatat
    10501 tgtaaccgtc ctctggtaaa tctaatttat ttaaaacttg ctttttaaca tcttcgcttt
    10561 ctgctagggc cactactgag ttagtatacg ttgacgctgt ctgttgagag tagtaaccct
    10621 catatgtaaa aaatgactca gaattatctt cttttctcgt tatataaaaa gaacctgaag
    10681 cagtgtaatt agagggtagg aaatgaataa gaatacctgt tattaacccg attaaagcac
    10741 ataaagctag tacttgtcgg tttttcctta tttttttaaa tatttctcta aattccatat
    10801 gtctaattat accagcaaaa aggttaataa aataagcgat aaaccgcaaa aaagtgatga
    10861 agttaagtat taaattatgt atactttagt aaatttatta aaatttaaaa cattactgcc
    10921 cagatgccaa aacaacaaac tataactaaa tcttctctca ttcccacggt gttgatatcc
    10981 ggaggtgcnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnta gcaaaaaaat
    11101 cttctgctaa atttttactt gcttccacga tagatattta tcaaggaagg ttgtcccaag
    11161 tcgatcttgg aaaatatttc ggttcttccg gcggtgagga aaataggttt agtttgctcg
    11221 aggcaaaaag atttgctgaa gccgtagttt gggagtattt caaaagacat aatttagatg
    11281 cccgtattgt aagactaccg gaagtttacg gtccaagaat ggacctttct tcgagtggtt
    11341 ttttaggtgg ttatattaaa aatgtattag atggctcctc aattgatatt tacggtgaag
    11401 gcgaggaaaa agaattttat ttgtatatct ctgatgtgat ttcagggtta gttaaggctc
    11461 agtttagcga gggtacttca ggagatattt actcacttgt acctgaaaac cccgtttcat
    11521 ctttggaaac cgcctatcta gtaagaagcc tggcggattc taagctttca atacaattta
    11581 aaaagggttt gtctgaaata agagagccgt tgagtatacc cgagagtttt aatgttaaat
    11641 ctattggatg gaagcctaag gttcaactaa aagagggtgt aatgaaaaca ctggagtttt
    11701 ttgactattc tccgaataca aatgctttta agccggcaaa atatttagag caaaaactta
    11761 aaaaagttga gtcagtgcct gaaagcatag tttctttaca gggagttaaa atatcaaacc
    11821 ttcctaaaaa tgatgagtcc gaaagatttt ttaataatac ttttaaagag actgccgaaa
    11881 atcaaattga accaaacaat gctttgcctg agaaaaaaat aaaaaataaa tttaaatttt
    11941 ctttatttaa taagtcttca aaaaaatcaa atataaagga ttttagtaat aaggttagaa
    12001 ccgacctagg ttattttaac gctatattgg gtattgtaat ctcggcgctt ttggttttta
    12061 tcggagttcc ctctttgagc acttatatta atatcaaaaa aggttactcc tctttaaata
    12121 aagtaaaaca aaatattatg tctctggact ctgctaattt aagctcagat accctctctg
    12181 catataacgg ttttaagaac ggaaaatcct cactaaagag aattagatgg gtatttaaga
    12241 ttatcggtaa ggatgaaaat tatataactt atgacaagct tttaagctcg ttaatgtttt
    12301 tttcaaaagc aggtaatctc ggttcagatg ctttgaaacc tgtggaatct atgttggaga
    12361 cgttgaagcc gggaagtaca ttgactttgg acgctactct gtttgatagg gcaaagattg
    12421 ggattgttgg agctagagat tatattagga tggctgaagc tgatttagtt ggtattgata
    12481 actctctaat tcctgaaaaa tacaaaaatt atcttgatga gtatcgagga gtactcagcc
    12541 agtctcaaga atttgcagat tccgcatctg ttgttatgag cgggcttcca caacttttag
    12601 gtatggaagg tgaaaaaaaa tacattattt ggtttcagaa ttccaacgaa ataagaccaa
    12661 ccggtggatt tatcggttct tacgctacag tgagtattga ttcgggaaag attaaaaata
    12721 tacttattga tgatatatat aaccccgatg gtcagattga tgttagaaat attaaaacta
    12781 cccctccaga cccgattaaa aattatcttg atgaggaagt actatatcta agaaattcca
    12841 attgggatcc cgactttacc aaatcagcca aggtttttga tgatatttac tttaaaataa
    12901 caggtgaaag tgtagatggt tacatagctg ttgatttgaa gtttgtcgag agtcttctta
    12961 gagtaacagg gccggtattt ttagcctcgt ataacgaaga tattaattca gaaaatcttg
    13021 acgaaagagc tcaatattat agtgggtttg attataaaga gggttcgaca gataaaaaat
    13081 cctttttgac aattcttgga gggaagcttc ttgaaaaaat attttctttg aaaaaagagg
    13141 attttcctaa acttgtaaca gagctgggga ggtcgcttga agagagacac cttcagattt
    13201 ctcttaataa taatcaaata aattctctgt tgtctgaaaa taagtgggat ggtagtttag
    13261 tcgataccga aggggattat ctatatgtgg ttaatgcgaa cctcgggggt accaaatcta
    13321 attattacgt cgaaaataaa atgacctacg aaattaattc tatgaccaga gatggtttat
    13381 taagagcaaa tttgtatttg gattataaaa ataatgctga aaacgatgct tggcccggcg
    13441 gtccttatac tgattatgtt agagttttag taaaagacgg ttctaagctg actggggcta
    13501 aaataattaa taatacaggt gaggaaacgg acatatttaa ggatgtagta gtttcgaagg
    13561 ttggtgttta ctcttctttt gaaactagtt tcaagttgct tccaaaagaa tcaattaggt
    13621 tggttattag ttacgatctt ccccagaaag cgtcaataac aaaagaaggc agctcatata
    13681 gtttgtattg gcaaaaacag cctggaaccg gtaaagacaa ttactcgttt atttttaacc
    13741 ctccttttgg ttcagtcact caaaacttat caaataattt acaagttgat ggtacatcaa
    13801 taaaatcctc gggaattctg ataaaagata tggagtacta cataagtttt aaataaattt
    13861 ttattgctta atgttttgtt gagcaatata attaccttaa tggcttcagc ttacatgccc
    13921 caatccaata gacaacatcc agttcctcag aatattatgg atgttgagtt taaacttata
    13981 ggtgagttga cgatgcgtca gttttcttat ctattagtat ttggtttagc ttcttaccta
    14041 agtctgaaag gaataccggg cctatttaga gtacccacag ccttgttttt tggtttactt
    14101 gctttgggtt tagcttttgt tcctgttggg gaaaggggtt tggacgagtg gatagtaaat
    14161 ttttttaaat caattaattc tcctactcaa agaaaatggt tgaaagagcc tcaattaccc
    14221 acggcgtttt tgtatgataa cttggctatt gttcagcacg agatgattac acttgctcct
    14281 acttccagtc gtagaaaact tgaagagtat ttaaaattta aatcttatga gagtgacgag
    14341 gatcccctcg atattcctga aaaatcctat gttatgaaac taaaacaaat ttatcaggat
    14401 acttacagcc ccgaacttac tggcggtgtt ggagtacttg tggacgagcc tgaagtatcg
    14461 gtaggtccgg aaatcccaaa tatcccgggt gttcctgtta ttccggagga tgcagagccc
    14521 aaatataatt tagaaaatga agaacctaat aaagaaacag ataagttaac ttcggaatat
    14581 aaagcatttt ctagtactga agattttggt aaggaagtta taaaagaagc taaggtaagc
    14641 gaagagttta aaaccgagca aacaccgccg gttcccccga ttttacaaaa atcaaaattt
    14701 gttgataccg gtgttaacag acaaaggatg agaaggggga gtgtttccga aaaaactagt
    14761 atgcgaaata tttctcaacc accaagcgaa ttggatatgt attcatacag ttcagttaca
    14821 cctgacatgc attcaggaag aaagtttact aatttggtac cttcagaagg tgagatagta
    14881 ttgccaatca ggggtgagag agtgttgagt acttccgtag agcagtcaat cgatagcgat
    14941 attcaggaaa aagccagaaa actcgaggag cttttacaaa aaattagtca ggaggagggc
    15001 gtggtaatta aaaaaacccc tgtccaaaaa cctgccacat ctccagtaga tagggtagca
    15061 gaggagatgg ctgagaaact taaaaaacaa aatgaagaac taaatgttga aattaaaaga
    15121 ttgaaagatc aaatacaaat gggcaagtct tcttcggtag aaactagtgt acaagaaaac
    15181 cttcttaaaa aactcgaatc tcaaaaaggc gatatatcat ccagttattc cgaattaaga
    15241 aagcaagttc aggacttgca aatgaaattg gatgaaaagg ttaaggtttc tacaggtgag
    15301 gagtttttaa ataaggttaa aaagcagctt ccggttttga cagacacacc caatgttctg
    15361 acaggtgttg taagggatgc agaaggaaaa aatttaggtg ataccctttt gatagtaaag
    15421 aatagcaggg gagatactat tagagcattg aaaactaatt ctctgggtca atttgttgta
    15481 tctacgcccc ttcagaatgg aacctatacc gtggaagtaa gtccggttaa caaaactaat
    15541 ttaacatttg ctataattcc tattgaagta aaaggagaag taatacctac attagatgtg
    15601 ataggaaaat aaatatgtct gcccaagaga ttatatctac aactcaagat catttagaca
    15661 tctttgatat taaggataat ttcgctgtat taaaaaacgg aacagtttgt gcagtcatac
    15721 aaactactgc agtaaatttt gacctgcttt cagaaattga acaggacgcg ataattgcgg
    15781 ctttttcaat gctattaaat tccattacat ttccattaca agtagtcatc agatcaagaa
    15841 aattggatat atcaaaatac gtagaaaaag ttagaaaagt agaatccaaa ataatagacc
    15901 ccctcctaaa acatcaggct gagtcataca gaaaatttgt tcaagatgta attaaggtta
    15961 atgaagtttt agataagaaa ttttatgttg tagtttcgac aactaacgta gttgtaactc
    16021 aaaaaggagg cggtgctttt gattggatgt taaagctatc tgggatgcaa aataaaagag
    16081 ttggtgttgt taatgtagaa aaaatattgc aggacggacg tacccaactt agccctagaa
    16141 ttgaccatgt aattagagaa tttggaagaa taggaattaa atgcaaacaa ctaaccacac
    16201 aagaattagt ggaactttat tttgatatct ataacccaag taatattcac ggtcagagaa
    16261 taagaaccaa tttagatgat tataagactg ccattgtaaa cccggcaatc atagaggaat
    16321 agaatatggc gttaaatatt tttgatttat ttaaaaaaag tacaaaaaaa actgtaagta
    16381 acacgggcga cacggctgag gtggagaaaa ctccacctga aatattggct gaaggaatga
    16441 tgaatgttag agatataatc gctccttcgg ctattgaagt tgacttcaat gatataagaa
    16501 tcggcgatat gtattacaga actttttttg tttccggata cccgagattt gtcggtgcaa
    16561 actggctctc cccagttata aattttgatc acactctttt gatttcaatg ttttattatc
    16621 cggtaaaatc aaaagtaatt ttagatgatt taagaaaaaa aataactgaa ttggaagcaa
    16681 caacaatgac taacagggag aagggaaaaa tttctgaccc tgtggtagag gcggcgcttg
    16741 aggatgcaac tgccttacaa gaacagttag tcaagggagt tgaaaagtat tttcaatttt
    16801 ctttttacat tactattccg gcatacgaga aagaagagtt agataatgtt agttctaagc
    16861 tcgaatctct tcttgcatct ttattgttga tatcaaaaac ttctgccttg caaatggagg
    16921 atgcatttag atcttgtata cctactggga tagacagatt gatggttaca agaaatatgg
    16981 atacgacaag tttagcaact acgtttcctt ttacgtcatc tgatttaact atggaagaag
    17041 ggataatgta tggaataaat aaacataatg gatcactagt aatatttgac cgtttctcga
    17101 tggaaaacgc taattcagtt gtatttgcta aatccggtgc tggaaaatct tacttcgtga
    17161 aattggaggc tttaagacac ttagtatttg gtgcccacgt gatgataatt gatccggaag
    17221 aagagtacta taatctttgc gaagttgttg gcggaaatta tattggtttt tcggctaatt
    17281 ctaaacataa aataaaccca tttgatcttt ccggaattgc tactgaaggg gagaacgaat
    17341 tgggtcagaa acttatttcc ttggtaactt tactaaaatt aatgatgggt cagctaaatt
    17401 caaccgagga ggcgattctc gaccgagctt taattgagac ttacagaatg aagggtatca
    17461 ccacagatcc tgatacacaa agtagtaaag aaccacctgt aatggaggat ttatacaagg
    17521 ttttattggg tggtagtgag cctgaatcta aaactatggc tgatagatta gagaaattca
    17581 ttaaaggttc gatggcaggg atttttgatt cgcagtcaaa tataaatctg gaaagtagta
    17641 tgactgtgtt ttcaactaag aaccttgagg atgcattaag accaattgct ttttatatga
    17701 tattggattt cgtctggact actattagaa gggatttaag gaaaagaata ttgatagtag
    17761 aggaggcttg gtatttaatg caaaatcccg atagtgcaag atttatatac ggtatagcta
    17821 aaagagccag aaaatattat ttaggtttga ctactatatc tcaggatgta gatgactttt
    17881 tgacatctga atatgggaaa gctgtagtta caaactcatc tattcagatg ttatttaaac
    17941 agcatcccgc agccatagat aaagttgttg aaacattcta tctttctgaa ggagagaaaa
    18001 gatttttact atcagcgggt gttggggagg gtctgttttt cgctggatcc aatcacgtag
    18061 ctataaaggt tatggcttcg gaatcagaac ataagcttat aactacaaat cccgaggaag
    18121 ttttaaaatt aaaagaagaa agacgacttg aagagaaaaa aggacagttg gatttggcaa
    18181 ggaaagagga acttagaatg gagtataaac cttataaagc tcctgaaccg atggacaagt
    18241 acacaacatt tgatgaatta ggggaaacta aacaggaatt taaacctatc aaaaattatg
    18301 agaatgaaaa tataaattct gaagttaata aaacggtacc aattgaaact aaacctgtac
    18361 tggagccaaa aagtgattta acaataaaat ccagaattgt tgacaaagtt gttccggagc
    18421 ctcagaaaac tgttcagaaa cctatgaatg agactaatct tcaaaaagat cagaagccta
    18481 ttgttataga tttgaataaa aaaaatcaga cttttgatgc tggaataact aacaatcccg
    18541 cacctacttt taaatctgca cctgctcctg aaaatgtctc tgtttctatg cctactccag
    18601 cgcctacact tacaccttca cctgttccta cacctacgca ggttattaat caaaataatc
    18661 aacagtctca accacctgca caaaaaccta aaaatatact cgacaataaa gtcattgagt
    18721 taaggaaaaa gatgttacag gacgaggata gtttcttaga taacgctcca tctgcaaatg
    18781 agattatgga ctctgtagag gagatgaaaa gaaacaaacc aatgattaat aaccagccgc
    18841 cgattcagaa taatccaaca atccaacaat aaaaactaga tttgcatcgg cataataata
    18901 tggatgaagt ctttaatccc gtccggtaca aatgtacagg gtgataagtt gccgttagat
    18961 ttaaattcaa tattttctga tttcacattt cccaagaaat ctaaaagata cttcgagttg
    19021 aatgcaattt ctacaagctc cccttcaact tcagcattta gattgctttc gtgctggcca
    19081 gtctcttctg aaagtgatgt gattttaatt tttccttcag gatcaaatct aactttgata
    19141 gtactgttcc cctcctttgc aaagatgtca gttaacctaa ccgcctctgt aaattccaaa
    19201 gttgaaaaat ttgctttgac tacgtgttcg gtcggtatta tctttttata atcaggatag
    19261 tctccatcaa gcactctagt ggcaattaaa gtatcttccg ctctaaacaa ccctaagttt
    19321 tcatcttggt ttaatgtaaa ctcaattggt tccgaagaag ttgaaaatat tttagctacc
    19381 tcaaggagtg ttttggccgg tattacaacc ttaaagtcgc taatctcacc tttaatttta
    19441 atattttttt ccgataatct aaaaccatcc gttgatgtta ttgttagctt ctctttcgag
    19501 tagtccatat aaatccctgt aaatattggt ctgctggtat ctactccgga ggcaaaagct
    19561 acaaccccaa ccacactgtt taataatccg ggatctatag ataaaccttt agcattttta
    19621 ggaaaagacg gtaattcagg aaaatctttt gcactgcttc cgttaaattt ggattttgta
    19681 gattttgaat ttacaacgag tatctcatct ttgacagttg cgttaatggt accgggcgac
    19741 agattagata taaaatcttt taataatctt gccggaactg taatagaacc ttcctcttca
    19801 atggatgcgc ctacatacgt agttattgcg gtttctaaat ttgttgccga cagttttatt
    19861 cttcccttat cggtttctat taaaacattt gataaaatag gcaaagaact tttagccggt
    19921 atggcgtgca ttatggtctg tagaccttta gaaagatttt cctgcaggaa agaaaatttc
    19981 attttttata tttattcctt tttaccttta tttaattttt ttggttttaa tacaactctt
    20041 ctatcctctc cttcgcccgt gctttccgat tctatatcat cgtattcaga aactagaaca
    20101 tgaatttgtc ttctctccca gggactcatt cttggcatct ccacatcttt ttgaaaaaac
    20161 ctgactttgt caatgaaggt tttggccatg tcctgaattt tttcggtttt tctacttttg
    20221 taatcattga tatctaaagt aattaaaggt tgtatttgag tttctctgta tatcattaac
    20281 cttaaaatat tttgtaatcc atctaaagat tgtcctctaa agccaattaa aaaatttaaa
    20341 tcatcccctg aaacagtaac attaaaatta ttttcctcaa tttcttcgac ggaaagttca
    20401 ggcttaatgc caatatactg aaaaatttta ttcatttttt tcttaattag ttcttttgta
    20461 tccatattaa ttagtaatta aggtaagtta gcagaataac tacgaactct ttttaccaga
    20521 tttcaactta ttaataaagg gttttaaacc accccagcct gagaagaaat aagtttgtcc
    20581 aatctgaaac actgttgata tgaagatata gagcataact cccgacggca aagtgatacc
    20641 gaatatgaaa aacattaagg gcatgatgta ttggttttgc ttctgcatcg cttgcatcat
    20701 atcgtctgcc tgtccgggtg tttgttttac agctttattt gaaacttttg tgaaaggaga
    20761 catcattttt gtggcaacaa attgagccac tacagtaagg attgtcagta tcaaataagg
    20821 atcaggtttt gttaaattta aatgtaaaaa ttttgtagca agtgcttcat cgtgtgcaaa
    20881 tttaaaagaa tctatataaa ttctactgtt tagagccact agatcgctcg cagttgtcaa
    20941 agtcgtcaaa gacctgtaaa tagcaatcat tagaacaaat gtaattaagg ttgttataca
    21001 gcccgcaccg gggttaattc cgtgtttctt gaaaagatcc atctgaagct cggcttgttt
    21061 tttcttatca tatttgaatt tttgttttat tttctctaat tcaggttgaa tgtccctttg
    21121 cttttttgcc atctttatag aaggcattgt agctgggatt agggcagcct tagcaattat
    21181 tgtgaacaag attattgaaa caccgaggtt gccgaatatc ctgtacaaaa ctatgagtag
    21241 attaaatatc ggatttatta atattgtatt ccacataatt atttaaatat ctttctaatt
    21301 tacttcacag gatcgtgccc acccttagaa aaaggattgc atcttagtat tctaccaaat
    21361 cccatcaagc ttccttttaa aactccgtat ttttcaatcg cttgataagt atattcggag
    21421 caggttgggg tgtacctgca cactttaaac cctttgttat caagagaaag atgcttcttg
    21481 taaaatttta ttaaattcag agctaatttc ttcatattcg acattagtac aggcagtttt
    21541 gggatgaata actacccata aacctttatc actaactgat tcaaaatttt ttctaattat
    21601 atccctaaat attcttttta ttctatttct tacaacagct cttttatcaa atttattgga
    21661 aaccaccacc ccggcttttg ttggtccctc gtaatttttt ggcttaagaa aataaatatg
    21721 aaaatattta cctttgtaat acttaccgta ttttctggta acgttaaatt caaaattaga
    21781 agatagtcta ttttcctttt taagcattag aaggtttaaa gagcggttta tcttttatgg
    21841 tctactttac ttctcttcga gacaacaagt ttttttcttc ctttagccat tcttctttta
    21901 ataacatttt taccgcccgg tgtggacatt ctttccataa atccgtgctt ttttattctt
    21961 tttctttttt taggctgcca tgttcttttt gttttcggca taattattac tccttaatta
    22021 caggtggagt ttataagata attgcggtat agtcaacaat atgaacgaac aaaaaccgtt
    22081 aataaaattt gatgattttt caaaagtgga catgagaatt ggttatgtag agagtgccga
    22141 ggaaattgaa ggttccgata agctaataaa aatgcaggta gattttgggg atatgggtaa
    22201 aagacaaata ctggcgggaa ttaaatctaa ttacactcct gcagatttgg tcggaaaaaa
    22261 acttccgttt gtggttaata ttgaacccag gaaaattatg ggaaatgagt ctcaaggaat
    22321 gattttagct gcagattttg aagataagcc tgtaatattc catttagaaa aagatgtaag
    22381 caacggtact ctaattagat aattaaaatg gttctccgac ttcgaaaatc aaagaaatat
    22441 tatacaaaaa catttggctg ttatgctaat gaagttgact ccgattatat tgcgggatta
    22501 cttgaggatt tgggtttttc tgaactaaaa aagaaaggac gtaatgagac cgaagagatt
    22561 gaatacgctt tgaaaaatgc cgatatcttg gttataaact cttgttccgt cagacaaaaa
    22621 agtgaggata aagtttacgg aatcgggaaa atgataaaaa ataatcctaa agcccgggct
    22681 aaagtggtgt taaccggatg tatggtcgga agtgtcaccg gagatagaaa aaggttttcc
    22741 gaaaaacaat taaaaatgaa aacaaaggat tttgatggtt atttccctcc atcagatatt
    22801 aaaagttttt taactgtact tagggaatta aatttaatat caaaagattt attttttgat
    22861 aaaaaaggtt tgaaaagacg acagagagac aagaaccatg cctacgtaaa tgtttcctat
    22921 ggatgtgata atttttgttc ttattgcgtt gtaccttact cacgaggttg tgaggtttcc
    22981 agaagtaaaa aagaaatact tgaagaaata aagtgccttg tagaaaaagg tgtttgtgat
    23041 tttacttttt gcggtcagaa cgttaattct tgggggttga gtatggagga gaagtttaaa
    23101 gtcaggtccg cgggacgcaa agatacagat aataaaaaac aaagtaagct accttttgct
    23161 caattattga gagaaattca taatatcaaa gaaataaata atattgattt tatttcatcc
    23221 aatccttttg atttcacaca agatttagtt gatgttttaa aacttcctaa gatttccaat
    23281 tatgttcaca tagctgttca atcagggaac aataagattt taaaggcgat gaatagaaga
    23341 catacttctg aagagtttgt aaatttagtt gaacggatta ggaaggtaag acccggtatt
    23401 gaaataggta cggatattat tgtcggtttt cctggagaaa ccagagagca atttatggat
    23461 actgctaaat tgattgaaaa agtaaagttt tctgtcgctt ttatttcgat gtattcttca
    23521 agaaaaggta cctttgcaga gataaactta aaagatgatg ttcctttaaa ggaaaaaaag
    23581 tggagacaca tgtatctaac aaaagaatgg cgaaaaaaca aaccaaaagt aaaaagtaat
    23641 tattcacact ctcgatgggt cagttctgaa taaaaaagta tgagcaaaaa ttcaaaaatt
    23701 tttattatct taggtcctac atcttcgggt aaaacatcta tagcacttga tttatgcaaa
    23761 atatttgacg gggaaattat ctccgcagat tccagacagg tgtgtaagtt tatggatatt
    23821 ggtacgggaa aagtacctat aaaatcagat ataaatctta aaaagtttaa ggatcgttgg
    23881 gagcttaatg atgtaaaggt ttggggatac gaccttgtga gtccagatga ttttttttca
    23941 ggatatgatt ttgctgatta ctctttaaaa aaggctaagg aaatattaga aaaaggaaaa
    24001 atagtatttt tagtaggcgg tacgggtttt tacgttgacc tttttaccgg aaagatgaaa
    24061 ccttcgaaag ttttgcctga tttaaatctt agagattcct tagaaaaatt aagtttgtcc
    24121 gatttacaaa acaagttgaa agcgcttaat gaaaaagctt tccaaaacat tgatcaaaaa
    24181 aataaagtta ggttaattag ggcaatcgaa aaagaattat ctatagatgt tagaaacgaa
    24241 caacttccct atcttgatga tgcaaaattt acctatatag gtttaacagg agatagagat
    24301 tatttatata aaagagttga taattgggta gatgaaattt gggatggtgg tttgatagat
    24361 gaggtaaaat cactagtgga aatgggttac ggagacagct ctaagattaa aggtatagtt
    24421 tacaaagagg tttttgatta catagaggga aatttaaaaa aatcggaagc tatacagaaa
    24481 accaaattca acctacattc ctatataaga aggcaacaga cttggtttaa gaaaaataac
    24541 ttaatagagt ggtttgatat tactaaggac gattttaaag aaataatata caatagaata
    24601 aaggagaact tataaatgga taaacaaata gtaatatcaa ttaaaactat aattcttttt
    24661 ctgctgacaa ttttaggtct ttttgttatt tacagattgg ggccaatcat aggaatactt
    24721 ttattatcaa cattgctggt tatatctatg gaacaacctg tgaggtattt catgagaggt
    24781 tttctattca ataaaagaat atccagagga cttgcggtct taatttctta ttctctgttt
    24841 gttttaataa tattaggagt tctcacattt gttgtccctc ctttaattac agagcttcaa
    24901 aaaatgattg ttagcatccc ttatatgctt caaaatgtta aaattccgca ggagtggcag
    24961 tcatccgttc cgaatatgat tcccgatacg tctaaaatat ctactggagt aataaatgca
    25021 acattttcaa ttttttcaaa ttttgccact ttcttgagtg ttcttgtcat atcaatttac
    25081 atgtcattgg attgggaaaa tatcaaaaag ggttttattt ctctttttcc taagaataat
    25141 gaggatatag tacaagagac tatagaagat atcgaaaaaa atgtcggggc ttggataaaa
    25201 ggtcaattac ttttaatgct ggtaattgga gtagcaagtt ttattgggct ttcaattttg
    25261 ggagttaggt attctgttgg tttgggttta ttgtcaggta tttttgaagc tattccgaac
    25321 gttggtcctg ttctatctgc tatagtggct ggtttaatag gttttacaga cagccccata
    25381 aaaggtattg gagtagtatc tctttttgtg ataattcaac agttggaaaa caacttactt
    25441 gttccaaaga ttatgggtaa ggtttccgga tttagacccc ttgttatttt gcttgcttta
    25501 cttgtagggg caaatttttt tggaataatt ggggctgtgt gcgctgttcc ggtaacaatg
    25561 atagcctcaa ctatcttgaa gaaatttttc gggacaaaca attaatataa ataggcacgg
    25621 gaagcctata agccagattc tgtattatcc gatcatctat ctacgcccca tctttgtacc
    25681 ttgccgagcc gactcaaagc ggtacgttcg tgggtttctg cagggtggga ttgcccgttt
    25741 catatctttt ccgtgcttaa gcttacggaa aaagctcgtc tctgttgctc tcggaagatg
    25801 ttaattcacc atccgtttcc attaattttc attaatgaaa caccagcgat tacgcgtcgg
    25861 aggttaccct ccgtcccctt gctctgtgca gtctggactt tcctcttcta atccgggttc
    25921 acacctttca taaaagcgat cagtcagctt cccgtgccgt tattataaca ttttctgtct
    25981 ttttttgttt tattaaaatt gtgatagaat gctagaaaaa tcttagaaag gatagaataa
    26041 atgggaaacc ttctttttaa aagaaaaaaa cgtaatcaag aaaatgtttt tagacctacc
    26101 gaggaactta ttctcgaaaa acgtctacaa gaagcattag cgttaaaggg gtataaactg
    26161 ggtaaagagc atcaggatag ttttcaaatt ataacctatt caattgtcaa acctggtttt
    26221 cttagcttcc tgtctatcgg atccataatt attcgcgagc agcttcaaga agtggagctt
    26281 tgggtttaca gattgggtaa ttattgggtt gatgatctga tggatatctt atccgctttc
    26341 gaaaaagaag ttggacatag tattcaaaag gaggtagtta caagttttta attatttctc
    26401 atctaacctt aatagcgtcc ggttttagat ttttctccgg acgctttttg tatgctaaaa
    26461 tacttgcatg tctgaagaat tagatccgga aaaatatcac gttttaacac ctaaaaagaa
    26521 aaaattagta gtgttttttc tattattttt attccttttc tttatacctc tgatgtctct
    26581 catttattat aagagcgctg ttcacagacc ttctcaaact gcaaaagaag ctaattttga
    26641 aataggatcg ggagatgatg tttttgaaat agctcagatg ctttatgaaa aagaggctat
    26701 aaattcagaa tttcttttca ttttttatgt cttcttgaat aggtccgata caaatattca
    26761 agctggtact tacaaaattc cagctggtac ttcattggta actttagttg acatgttaaa
    26821 aaatgggaga aatgatatat ctttaacatt tttagagggt tggagattgg aacaattcgc
    26881 aatttatgct tcacgaaaac tcaaaaactt taactaccag gattttatgg agattgctaa
    26941 accgtatgaa ggttatcttt ttcctgatac ttattttttt aatttagatg cagatgagga
    27001 ggaagttttt aataagttaa gagatacttt tgataagaag actacgaata tcctcacagg
    27061 tgaaaatttg gataaggtcg gtttatcaaa acaggaagca gtaatttttg cgtctatggt
    27121 tgaaagagag gtctctaacc ctgaagatgc tcctattgtc gctggtattt taataaaaag
    27181 atggaaagaa ggtatgaaat tagatgtaga cgctacaact caatatagtg ttgcgtataa
    27241 aagattgtgt ggtggtgatg ggggcgacat aatatgcact ccaactttag aagatatgta
    27301 cgatattgat tggtggcctc acaaccttac ccgcgaggaa ttagacacga aaaatcctta
    27361 taacacccga gctgtggttg gtctcccacc gactcccatc tcaagtttta gtttatcgtc
    27421 actatctgcg gttataaatt atgagaaaac tccttattac tactatttaa ccgataaaga
    27481 cggaataatg agatacagcg ttagtctaag tgaacacgag caaaatattt caaactatct
    27541 ttcaaattga ttctaattaa tccgaatcct taagactttt aattattaaa tctttggtaa
    27601 tttttattcc ataattttcc aatattttat ctttaacaaa gattggaata gctgcctctt
    27661 ttgaaatttc tagtgcgtct tttattccaa tattaatgtc aaagtctcta ccttcttttt
    27721 tcaaggtaag ataagcgtaa aaattattac agtagcagtg ataaattttt atatgagcaa
    27781 tatcaattcc gcacccatat aaaattcttt taatgctgtt atagattgat ggggtcgtgg
    27841 tcttgttatt aaatataatt tctatcggca gtagagtttt taaggattta caaaataaat
    27901 acagtgtaga aaaatctgtg taaggaacct cagttataaa ttttactggc ttatacatat
    27961 ttttataaat gtaatatata attgttataa atatgtctaa tctgctcaaa aatacttatg
    28021 acaaaatgga agctattaag ggcaaaaggc tcgtttatat gcttttagcc atttttgtct
    28081 cttttaccac agtaggtatt tcaattggat atcttatgtc accgaaatta aatgaagatg
    28141 aaacacctgt gaagacctat tcgggaaatt tgccaaaatc cgagaagaca gaagcttccg
    28201 ggaaaattac ttatgtgaat cctgaaatgt atccaataga tgaaatatcg tattctctta
    28261 ctgatggttc tggtaaagat atttatttat tatcagcaaa ggataataaa ttagaaatat
    28321 ctgaagggtt gtatgtaaag gtaattggta ctttaagtac attaaaggat gggaaaactc
    28381 ctgttctttt ggttgaggag gtaataataa acaatgtctc aaattgaatt taaaaatgtt
    28441 actaaagaat atattcctgg taataaagtt ctggatgatg tgtcattttc aattgaaaaa
    28501 ggagaattta tttttataat cgggccttcg ggggcgggta aatcaacaat tattaaatta
    28561 ctaactagag aagaaaaacc agaagaaggt caggttgttt tcaataatca ggatatttca
    28621 aatattaaac ctgaagaaat gcctgaattt agaagaaaaa taggtgtagt ttatcaggat
    28681 tataaacttc ttaactccaa gactgtatat gataatgtgg ccgtatcgtt ggaagtggtt
    28741 gatgctgacg agcaggaaat taaaagtgta gtcccaaatg ttttaagtct ggtaggtctt
    28801 ttagaccatt cttcatacta cccccaagag ttatcgggag gtgagaaaca aagagttgct
    28861 atcgcccgag ctttagctca cgagcccgat gttttaattg ctgatgaagc taccggaatg
    28921 attgatcccg atgtttcaga tgaaatagtg gagttattag aaaaaattaa ctctttgggt
    28981 acaactgtta ttatggcaac acacgatcaa agaattgttg ataaattaac taaaagagtt
    29041 attaaaattc agaagggtaa actagtatct gataaaaagg gaggtaaata tcgtgcctaa
    29101 agtttggaac atattaaaaa actatataca gaaagaaaga ttaatgacgg tttcaaacat
    29161 tttagttatg ggagtgacct tcctactcct tgggattttt attgaagtgg tagtgttgtc
    29221 ccaaacaatg ttgaaatatt tagaagacca ggctcaaatt accgtatttt ttaaagatga
    29281 gttcggagaa gataagataa ttccttttaa agatagttta ctttcagacg atagaatttc
    29341 tgaagtaact tatatttcca aagaggaagc cttgaagatt tttaaagata taaatcaaga
    29401 tcaaccggtt ttattggaat ctatttctgc gagtgtgttg cctgccagtt tggagattaa
    29461 gacaaagaaa attactgatc tcgaagggct tacaaacgag cttcaatcaa aagagggtgt
    29521 agaggaggtc aggttctata aagatgttat tcaaaaattt aaatcggtta gtaacgctgt
    29581 gtatatagtt ggttttattt tagtgattat gttcttccta atttcatact cggtgattat
    29641 ttcttcttta aggacagcaa tagattcgcg tggccaggaa cttgaagtaa tgagattagt
    29701 aggagccaag gattcttaca tgacgaaacc ctttatgtac caaggagtgt tttttagcgc
    29761 agtgtcatcc tttttatcat cgattgtaat cttcatttta gggtttgttg aaagtaagtt
    29821 aggtgtattt tccaggggtt tatcctttgg ttttttacct aaagtattca tgaatcccgt
    29881 aatattttct ttgattttag cctttattct catactttcc ggatttgttt tgggatactt
    29941 cggaagcaag gctgcggtca agaaatattt aaaatattaa aagacctatg aaaatcaata
    30001 aacatttttt cttagtaata gctgtaattt ttgctttttt gctacctttt actattcctt
    30061 cttttgccca agaagaaact tctaacgagg atgaggaaga gattcaagat attcaggaaa
    30121 aaatagaaaa atatgagaag aagattagtg atttacgagg aaaagccaac actttacaaa
    30181 atgaaattga ttatatggac agtcaaattg gtcttactca gttaaaaata caaaattcta
    30241 ttgccaacat tgtaaaaaca gaagatagga ttaaagagct ttcagagggt atagaaaacc
    30301 ttggtataag aatagacaag cttgttaaat caattgaata tcaggaaatg attttaggct
    30361 cgaggatgag agaaaggtac aaggatagag gaaatagtat gctcatgctt ttatttggaa
    30421 gcaatacttt aaactctctg gttaaaaaaa cagagtactt aagagcttta gaggaaaatg
    30481 ataataaatt aattgatcag atggcggaaa cgagaacaag ctttgaacag cagaaggata
    30541 tttttcaaaa caaaaaaaca gagcaggaag agttaaaaaa caaacttcag attgaaaaag
    30601 ctaacttgga tagttaccaa agaagtctgg atagtcaaaa agaagcaaaa gagaagttat
    30661 tggccgatac tcaaaacgac gaagcaaaat atcaggaatt gttggctgaa gcagagaagg
    30721 agcttgcttc ttttgcagga ttcgttcaga gcgctggagg aggaatcatc ggaagtaacg
    30781 aatttggttc gggaaaggaa ggttggtatt tgtcccaaag ggatgcaaga tgggctaatc
    30841 aaaaaatagg aaattcaaac tatacagttt ttgaggtggg gtgtttgatt acaagcgtcg
    30901 ctatgtataa cagatattat ggaaataaca tttctccggg tgacgtagca aaagatgata
    30961 gcaggttttt ttcaaatact gcaatgatgc ttataccttg gaaagcacct cccggaaaga
    31021 gttacaaagc tattaccgca tcttccataa gtgatgagtt agagaaacgg cctgttattg
    31081 taggtgttta cgcaggtcct tatggtacac attttgttgt actttctaaa aaatcaggta
    31141 gcgattatat tatgtatgat ccttggtacg ggcctgatct gaagttttcg gattattatc
    31201 aaaaatcatc tatctacagt gctgttgttt ttaaatagaa tatcctacta aaaatagaaa
    31261 tttgcatctt ttgatcttta ttttttatcc tagaatcaat ttgtgaggaa gatgatattt
    31321 attgctctaa tagtattcta cagaatattg tgtattgctg aaaataacta ctttgacttt
    31381 tcattaaata gatatcttat agatgaaaat aaagaaacgg tggtcaattt taagtataag
    31441 ctggaaggtt acgatgaaaa attatttttg gttaaacctt ttatagaaaa tggaattgtt
    31501 gaagtattta atgcggaaaa aaatctatgg gtttcatcct tttcattagt ttcagatctt
    31561 ccttccctgc aaaaagaaat gttaataaga ataaaaggtt ttgggataga gaaggccaat
    31621 ctgtattttg aaatattaaa tttatctaat ggtcaggtat atctgactcc taaaaagtat
    31681 gtttggtcca aaaaagtgta tgaaaaatat ctggaggatt taaataaaaa aattaagggg
    31741 gatttagtat taaaaaacga aggtgagggc gtccaagaag aaactttaga tagtgtattt
    31801 ttaaaagatt ctagtagaga aagtatttat aaaaaattat atgaagaaat cacggaagat
    31861 tgtttttacc ttttagtatt tgttgaattt ttaattttct tttatttagg tttcaggaag
    31921 agtaaaaaca gtgttaaaag aattgatatt gagtcccgaa cttatggtgt aaatggtaaa
    31981 ataccatagt gaaaaaaatt attaactggc tcccggcttt agtggtgatg actataattt
    32041 ttatactttc ttcccagcct ggggaggtgg ttaattccac tgtggcaaat acagagtctg
    32101 tacaaaaaat tggtcatgta ataatgtcta tgattctctg tgtttcctat tttaaagcca
    32161 caaaaaacat acccctttct atattgcttt cgcttctgta tgcaattttt gatgagtatc
    32221 atcagtcatt taccgccgat agatcttcca gtattagtga tgtatttatt gatgttctgg
    32281 gggcatcagt ttcgggaata tttttatgga aagtattacc aaatctaccg aagaaactaa
    32341 aagaattgct attgaattag ctggtaaagt acataaggga tctgttttag ctttgtatgg
    32401 aaatcttgga agtggtaaaa caacttttac cagatacttt gttgaagcat tgggtttaaa
    32461 taatagggtt caaagcccaa cttttgtggt agctcgaaaa tacggttatg taaatcacgt
    32521 agatctttac agactaacta gtgaaaacga agccaaggat attgggattg aagagatgtt
    32581 aacagattcg gattctttaa ctttgataga atggccagaa ataattgaaa atatattgcc
    32641 taaaaattcg gtaaaaattt attttgagta tatagatgaa aatacaagaa aaatcacaat
    32701 tgaaaattta cattgattcg tctcaaagat acatgaaaaa agttagtctt ttggagtgtg
    32761 ttggagatac agaaaagaag ttagatttag ttgaaggtag tatagatgtt gttggggcaa
    32821 taagagattt gttaaaaagg aataagttgg aattaaaaga tatttcggag tttgaaccaa
    32881 tgctggggcc cgggtctttt acaggtttaa aggttggagt gacggtttca aatatgttca
    32941 attggttgtt aggtcgtaaa gatttaaaaa atcttgatct tcctgaatat ggtggggaac
    33001 ctaatatcag cgaaccaaaa agaaaagaac ccatcgggcg ttaatgggtt ctttgtttat
    33061 ttactgcttt ttgaacagag tgtttgaatt ctcatcttca aaaaaatcaa ttccaaaagc
    33121 gtttctcagt gaatttttaa tccccttcct gattttccat ggtaatgcgt agaaattgtt
    33181 agcctcaatt tccccttcgt tataaagaag ctcgatgatt tcttcaacta acaatttgat
    33241 gctttcttca agatttatct tttgaagagt ttcattctct tcaatcccat caactcgagt
    33301 ttttatctca ccaagaaatt ttttaatatc ctcaaagctt ctctgattag aataacgttc
    33361 tgtcattttt acctcttttc tttggtgtta ttttcttcgt cagcttctaa aaaactcagt
    33421 atgagtttca taaaaatatt aacccgatat tttgatttcc aagacaatgt gggatatgta
    33481 taatctttaa ttaaccctag gctccagagt aataacagca cctctgttag agatctcatt
    33541 tgttcattat aaagattata gtaacctaat ttgccctccc tctgtttagt tctttcaatt
    33601 atcgaagata cttcttgctc tagtttttta gctcgtctaa cttctgcagt actcgctgag
    33661 ttattttcgc gtgccatcat ttttctcttc ctttctctag ttttttaaga ttctatcacc
    33721 aagttaaata aaaagccaag agatgtataa tatataaata tgaaaattct tgtcaccggt
    33781 gctgccggat ttataggttc ccatctctct gaaaggttag ttgatttagg acacgaagtt
    33841 gtcggaattg ataattttaa tccctactat tccgtggaac ttaaaagact taacgcttca
    33901 gatcttgaaa aaaagggtgt gaaaattcat gaatttgata tttcagaaga tgtgatagac
    33961 ccaatctttg atggtgtaga agcagttttt cattttgccg cccagcctgg tatttcagct
    34021 tcaacgcctt tttcaacatt tatgaaaaac aaccttgtag ctactgaaaa tatgtatcaa
    34081 gctacaaaag atcttccgaa ttttaaaata tttgtaaaca tatcaacctc ttctgtttac
    34141 ggcaaaaatg ccactgatga tgaaaacaca ccccccaagc cgacttctca ttacggggtt
    34201 acaaaacttg ctgcagagca acttttattg gcataccaaa gagatgttgg atttccagct
    34261 tgttcaacaa ggcttttttc aatttacggc gaaagagaaa gacctgataa gttgtatccc
    34321 aaggttattg actcaattct taatgataga ccttttccgt tttatgaagg tagcgagaag
    34381 catctaagaa gtttcactta cgttggtgac attgttgatg gttttgtagc aattttaaat
    34441 aattatgaaa aatgtttggg tgagattttt aatttaggtt ttgacggtgc cattacagtt
    34501 ggggagggtt tgaaaattgt tgaagattat cttgggaaaa aggctatata tgatataaga
    34561 ccacccaggc ttggggatca gcttgagact agggcaacaa ttaaaaaagc gagggaagtt
    34621 ttagggtata atccgactac cacacccgaa gaggggt
//
LOCUS       DAOTFA010000002        21887 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate AMR_MDS_3115
            NODE_1576_length_21887_cov_7.734472, whole genome shotgun sequence.
ACCESSION   DAOTFA010000002 DAOTFA010000000
VERSION     DAOTFA010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868139
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (wastewater metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 21887)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21887)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:36:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 536
            CDSs (total)                      :: 521
            Genes (coding)                    :: 520
            CDSs (with protein)               :: 520
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21887
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1576_length_21887_cov_7.734472"
                     /isolate="AMR_MDS_3115"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..371
                     /locus_tag="P5014_00200"
     CDS             <1..371
                     /locus_tag="P5014_00200"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HRY56569.1"
                     /translation="SKYGVVTYKTNVPYKYEIESIVEKPAMEKAPSRMAQFGRFVLSY
                     DVVIEAEKRQLGKGNELWLTDIINNIAQRGDKVIAQPIDGEWLTTGDPLRFIQTTLKF
                     AMKRDDLKKDLTEFIKKEII"
     gene            408..1139
                     /locus_tag="P5014_00205"
     CDS             408..1139
                     /locus_tag="P5014_00205"
                     /inference="COORDINATES: protein motif:HMM:NF020700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRY56570.1"
                     /translation="MTAASSGIQQKRISEKELRDRLRKRMLGIWSIVIIGILSLFLFA
                     PQVGSLFGYFSKYRNDPGYVPTPKPIPPVFIDAPKAINKESVTLTGRALPGSFIKIFV
                     NGPEKGSTTAGNDGIFTFSDINLNVGTNTIFAKSYDDKGNESESSEFLIINYDKDAPK
                     ITIEKPKDGDTVKNLDKRIEIEGKIDEKATITINEKTAIQKSDLSFSYFMSASEGDVE
                     IKIEVVDIAGNKSTKELKVKYVKGQ"
     gene            complement(1117..3210)
                     /locus_tag="P5014_00210"
     CDS             complement(1117..3210)
                     /locus_tag="P5014_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56571.1"
                     /translation="MKPIIIEILNRLLLLFILVVPNIYLVLILARVKKSLKSLLIPAF
                     CMSLLILIFLNKLIFTSQTLSAQDFNNIQIPFFHFFKESIVNYFEPPFWNSRFGGGFD
                     AFSNPISAYFAPLNFIFLIFKNVYEGAGVFIYFQLLLTSLFSLLLFKELKFNNYLSFF
                     GAIVFTFNGFVTMRLSPGVGIEYLYSYKWIPLIMFFTLIYLRDRKLHDLALLSISLAF
                     TLEGNLNIVISIWAFWLLFLLFMSKNFIRDIRHYILIPLLSMLLFSIKILPFLDLMRN
                     STGRISGVVTGWRLNKIDMIDFLSYFPPIRQLFGSALFTPGIIAIAFFFLSVVYIIFS
                     IFSKRKIEKIFVFLICSIIFGFIINTYNPLSNILFSLPFFNRITIVPAFMVFIFTPVY
                     ILSVYGLEMFKNYVLSRRFLKNFKNLDKFGIVCISLLVFAEILTGPSTFGNNTYSFNF
                     AKMDKTEVNKVPPYNTIKNLRPGVFAFIDSSDTFLYPHVISNIDLYTLNNFKYFYSPS
                     SERNLIKEGVFKDIVNRSDYIISTKPLNEKEVSLISEVGTADIFSNFKSYSILDKKYD
                     YLDLQAKNDWNELMYIYSVETSDLQKPYFKKLDNHPTKLSISKVDDYKVDNEIKTSVR
                     YSRWWRDLNVNKVILSEDEFGFMVISEVGERDKVDLFYLNPYIYAGLFLSLLSSIVVF
                     YLWRGSAETIVPSRT"
     gene            complement(3234..4454)
                     /locus_tag="P5014_00215"
     CDS             complement(3234..4454)
                     /locus_tag="P5014_00215"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRY56572.1"
                     /translation="MLVASFYITLFLLSLNQLSVLSKSGGVSVYLFDISVILFSLFGT
                     CYFLILKKSFKIPRYAYLYVFFILPAGISLLLSSIEFSTDKIMTGFFYLLRYFSYLLS
                     GVVVYNMVDKRLLSKKRIEDAFILSGIFIAIAGFIQLILLPDFTILDPSLGWDPHKNR
                     LASTFFDPNFVGAYFVICLAVLLERIFPDKCTKESSKTGFYTYFCLIVILIGLILTFS
                     RSAWGMMGIVVLAYGVFRSKVLLLVAFFTAFMAYFAIPRVQTRISGTTDPADSASLRI
                     VSWGNTVRIIKDNIWTGVGFNTYRYAQENYGFLTPDQDEIHSGAGSDSSLLLVFATTG
                     ILGFLIYFVAFLFPIIDSLKKRYLGWLVILGILISLLIESQFINSLFYPQIMFILFPI
                     LFIFDNRLQYLSVL"
     gene            complement(4441..5730)
                     /locus_tag="P5014_00220"
     CDS             complement(4441..5730)
                     /locus_tag="P5014_00220"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase"
                     /protein_id="HRY56573.1"
                     /translation="MKLNLKSNTATKIASNTVYQIFGKLISMSITMLAVVIITRSYGR
                     EGYGAFSLMQSWPALFFVIVDFGINAIAARDITEDWSKAAKYLGNILIIRFLFSLFLI
                     LIISFSISFFPYTSDLRNGIRLGLFLLITQSLFSTTNIFFQVKLRYDYSTIAYVGGYI
                     LILVLVLISSYFKLPVTWANFSYVIGGLLTFILSMSYLAKMGIKPDFSLDKKIWKYLI
                     LSALPIGIMFLFSQMSFKEDALMLSFLKLPKSYGLNNTESVAVYALPYKVFEVMLVVP
                     TFFMNSVYPVLVGHMVEGEQKLKKTFARVMYFLTGSGLIIGLLGVVFSSLSMKILGGE
                     EFVQSVGVLNILSGGLFIFYLTSPISWLIVTLGYQKYLPWIYFVSFSFNIIMNFIFIP
                     KYSFYGASWITVLSEFIVLILLIIFARKSWRLKYARS"
     gene            complement(5727..6953)
                     /locus_tag="P5014_00225"
     CDS             complement(5727..6953)
                     /locus_tag="P5014_00225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56574.1"
                     /translation="MDISKTKRKFWSFFLLSLILFGVLSYLFYKNIVTSKQKKLEKDI
                     ENIEMDFVFKSEDWWEQEEECTAIQNIEYYYYSGDDPLWKENNKFGLYIYAEEKDFFE
                     IAQNLINSKGGEWGYVLIPYNVKDYDEGKWGRVFDQLISKKLIPVIQLWNFDKDHYQK
                     NTKRAAEFLNSFIWPIKYRYISVYNEPNDDKFWVGDADPEEYAEVLDFTIKTFKETNP
                     DFYMLNGALNISSPTDGQNIDAFEFMRKMNEKVPGIFDKLDGWASHPYPQPNFSGNPY
                     DKGRWSIRAYEDELKYLKEDLGVKKDLPVFITETGWAHAEGDNYNPSFPKVETVSEYY
                     KIAFEEVWLKDDRVRAVMPFTIRYDPPFDHFSWINKDKVPYLQYEVLKKIEKVKGKPP
                     ALMTDNVDIYNCPGSK"
     gene            7017..8258
                     /locus_tag="P5014_00230"
     CDS             7017..8258
                     /locus_tag="P5014_00230"
                     /inference="COORDINATES: protein motif:HMM:NF012907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase family 18 protein"
                     /protein_id="HRY56575.1"
                     /translation="MKTKKILIISIFVISALLFTASMSVKKTGTPKNIRSSKVVSPFS
                     SNINVLSSFSKISEKPRKLIYGYLPYWNITKIEYLQLDKLTDIAYFGLYLNEYGDFMT
                     TTIGEDGTTIIEPAYNNWKNSDELNLLITHCKENNVRFALTIIAHDDASNDEFLNCRE
                     CWDNLVKNLRTELDRKGIRDVNLNFEHAEGTKEGIDKKFSEFTKYLNGELDKIYGNSY
                     VVVSAFGDSATQNRVSSDLENLSRAADGIFIMAYDYHQPTSEEIGPVSPIEGPGIDIK
                     ETINDFLTKVPPNKIILGVPYYGYNWLVEDESQYSKRKDGSDENGYSQSQSYEDILGT
                     ILELNPVLKWNEEGKSPYFTYVSPETGQLRTTYYENAKSLKYKYDIVNEYNLGGVGMW
                     ALGYDGGYTELWNLLYDYFIK"
     gene            complement(8251..8703)
                     /locus_tag="P5014_00235"
     CDS             complement(8251..8703)
                     /locus_tag="P5014_00235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56576.1"
                     /translation="MIYIKYFIFCLLVAILLALFEIQTEGKHGWAKKLPTWKKSFKFL
                     DALPGFRGPITGYHIYLFTMILFLVHIPFLFMKWDLRTEVIILSGYTLIAMLEDFLWF
                     VFNPHFGLEKFKEKHISWHEDWIGPMPVQYIYVFVIWVTLFVLGFFII"
     gene            complement(8846..8922)
                     /locus_tag="P5014_00240"
     tRNA            complement(8846..8922)
                     /locus_tag="P5014_00240"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8886..8888),aa:Val,seq:cac)
     gene            8993..9535
                     /gene="lepB"
                     /locus_tag="P5014_00245"
     CDS             8993..9535
                     /gene="lepB"
                     /locus_tag="P5014_00245"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HRY56577.1"
                     /translation="MFDKNVKKVIVEYFQIIFIFLIVFAIIYFFVGQLFEVVGDSMIP
                     NFRDKEQLIAEKISININNTERGDVIIFKNINENNRLLIKRIIGLPGENIKIQDGKVY
                     INGVELSENYLEEGTVTEGKRILEEGPEYKIPNDSYVVMGDNREQSNDSREWGYVKKE
                     NIVGRVVLVYYPLQNFRFVK"
     gene            complement(9557..9829)
                     /locus_tag="P5014_00250"
     CDS             complement(9557..9829)
                     /locus_tag="P5014_00250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006590091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HRY56578.1"
                     /translation="MAHTKAGGSKAVQGGNVSGKRLGVKVYGGSAVKTGQIILRQRGR
                     EIVPGDNVSMGKDFTIYSKTDGVVDFSWKTKSKKKVSVLEKKETKK"
     gene            complement(9831..10325)
                     /gene="rplU"
                     /locus_tag="P5014_00255"
     CDS             complement(9831..10325)
                     /gene="rplU"
                     /locus_tag="P5014_00255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HRY56579.1"
                     /translation="MEKYAVIKLGSKQFLVQEGDVVELERQEKPLRVDVLFYTDGQKT
                     IIGEPEIKDISVRAEVVEEKLGKKVRVARFKKKSRYERVRGHRQPMSIVKIEKIGDGK
                     SAKEEKEVKAEKVSEKPEIKEKKTVAKAVETKKPVTSEKPKKTTPAKTPTKKPVDKKL
                     KGTK"
     gene            complement(10376..11779)
                     /locus_tag="P5014_00260"
     CDS             complement(10376..11779)
                     /locus_tag="P5014_00260"
                     /inference="COORDINATES: protein motif:HMM:NF024791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HRY56580.1"
                     /translation="MQDLVVSINKNILKISTIDKEAQLKTAMLNVPKELVDDTRIIDP
                     KGFSNLLEKSISQVSVLSKNKLGLNFVMEPQDIFLRYVTVSKNGQETGEQIISEIKAK
                     DPDIKLDTLYFSYKKMAPFVYQFVGIRKEVMDNYLETSNALNIGLKSIIPWVLAFPKY
                     ENVNDPAIFVSKVDNDQVIALSELNGIFFAGTYKKEKTSEELTSLVKELSFYKRSSPI
                     KCIFTFNCDSFTMPNYEIKKIESPKFNGNFNLPEGFEINNIVNFLLDSDPTILGGQIN
                     VLNMLPLPVVERKGSALLVTGSVIGALLLLVGAYFGITSLGNKKAGNEQLAQNNTSQE
                     TQVLSETTSQEQPVQEEQSQQGTENENPEKDPNVEVKRGNLKIRVENGAGVGGLAART
                     KEYLEGLGYTVLTIDTADSKVQPTVLQFKSTVQKEFENLVKEDIKTKFPEIEVKDNIS
                     EDSDYDLLITVGTSSEL"
     gene            complement(11798..14656)
                     /gene="ileS"
                     /locus_tag="P5014_00265"
     CDS             complement(11798..14656)
                     /gene="ileS"
                     /locus_tag="P5014_00265"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HRY56581.1"
                     /translation="MSFKLPDKKQIPEMEDEILSYWKKNNIFKKSVEKNPEDNLYVFY
                     DGPPFISGLPHYGHLLVSIAKDVIPRFWTMKGKRVERVWGWDAHGLTVENRVQEELGI
                     ENRRDIESYGLEKFTQACYEYTSRISAEWEWYVDKIGRWVDFKNAYRTTDQKYMESVM
                     WAFKQIYEKGLIYEGTRTSLFCPTCGTPVSDFEIAMDNSYREENDLSITVRFRVITEG
                     DFKDYYLLAWTTTPWTLPSNRALVVDDKETYVSVQFENNTYILAKKRLDFVFSDKNYE
                     IKEEFLGKEIIGLEYEPLFTFYASKEGEFKVYSYEGMVTMEEGTGIVHSAPGFGEVDT
                     EMGKHFNLTLMMTLDDEGKMLPGNQDSNPYEGMFYLKANAIILNDLVEKGVIFKNEKA
                     VHRVPYHDRCNTLLVQKAQNSWFIDIQKIKDELIENNEDINWVPEYVKHGIFEKTIEQ
                     APDWCISRNRFWATPMPVWESKDGDRLVFSSIKEIEEASGQKVKDLHRPYIDEIVINK
                     NGKEYRRRPEVLDSWMEAGSMSFAQLHYPFENREKFEKNYPGDYIVEYKGQVRAWFQR
                     MHIISTLIFNSRCFNNVIVTGVMAGTDGRKMSKTYKNYPDPRGIMEKYGGDALRLYLM
                     GSSLMSGENTNFDEIELKNKSRNILNPLWNSAVYFLMYAQSNNWSSENKVISSNVLDK
                     WIIIRLNQTISDIDKFLKLYNVPPAVKSLEGFIDDLSRWYVRRSRDRISSGDNEALST
                     LYTVLLEFSKASAPLIPFISENIYRTLQEENLDEKSVHLCDYPVFNNDLTDESQEILI
                     NMKKTRDIVSQVLSVRVEKSIPVRQVLGSSAILKENDVPEEYKKLILEETNIKNIEVV
                     DSLDEKPMWETDLTNSIKLNIEITEDLLKEGKAREFIRNVQDIRKEMGLQISDEIVLT
                     YQDSSDMSEIISLYGDEIKNKLLAKEIIPGEEIKVEKL"
     gene            complement(14828..15379)
                     /locus_tag="P5014_00270"
     CDS             complement(14828..15379)
                     /locus_tag="P5014_00270"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HRY56582.1"
                     /translation="MYKIYTKEGDLGFTSLSNMFKKISKTEFIFDVLGTFDELEVSLG
                     FLHSARLDDVRKVAKEVQKDLFSLGSVISSNERISKESVEKWENRIKELEEVIDYFNN
                     KNDPLESFILPGGCRESSFLHLSRVTCRKLERMVFQYLKKDKNKLFIGKYLNRLSDLL
                     FVMARYSNKKLGFKDIIWNNKES"
     gene            complement(15465..18044)
                     /locus_tag="P5014_00275"
     CDS             complement(15465..18044)
                     /locus_tag="P5014_00275"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR02504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HRY56583.1"
                     /translation="MNSYLYFNDESDLQKNEKIDFSENGRTILEKRYLRRNKLGEIEE
                     TPEDMFQRVANAIAEPDEPYRNPEKTSIEFYNLISTKKFFPNSPTFTGAGTPIGQLAA
                     CFVLPVEDDLGKSRDGIFQTLRNAALIQQSGGGNGFSFSRLRAKGSRISTSNGLSTGP
                     VGFMEAYNGAFGVIAQGGVRKGANMGILRVDHPDIREFITAKTTEGKLSNFNISVALT
                     DKFLEAVEKDETYEIFDPHTKEIVETPKAREIFDLIVENAYKNGEPGVLFIDTANKDN
                     PVPSLYELEATNPCGEQWLGPYENCCLGSINLSEHITSDNKIDWEELRKTVILSTRFL
                     DNVVSANKYVPAVPQLKEAAHQNRRIGLGIMGLADLFYVLGVRYGSEEAQDLAGQVME
                     FIRFHAMKASIELAKERGPFPAIKDSIFNPSNLVWTPPTPLKSFSFGWERPAIDWSEI
                     TNGIRLYGLRNAAVTTVAPTGTISTVAGVEGYGCEPVFALAYLRNVYQAAGDLEKMTL
                     TYVSPSFQKALDDIDMSDVKRREIIDEIIRVGSCQSIKDLPDDLRHTFVVSGDITPEE
                     HIYMQASLQRFVDNSISKTCNLPPTAVPEDVEKVYMMGWKLGCKGLTVYVSGSRQEEV
                     LVTQEKKESTTEGGKSDGEKPVEEGKIDANKDVSKEITNNEEKLIVEKPSDVKLEREY
                     RLNGSTYCVKTPQGKAFITINKDSNGRPFEVFINVGKAGTDVSALSEGLGRLVSGWLR
                     VPGASENAVTEIISQLGGIGGSRSVGFGKNKVSSIPDAVAKVLSEELGYSLLKNQSNG
                     NGDNFISTKINTEYLNIRSDASEVSLNPVTGNIDVCPDCGNYSFIKEEGCAKCYLCGY
                     SVC"
     gene            complement(18264..19409)
                     /gene="ftsZ"
                     /locus_tag="P5014_00280"
     CDS             complement(18264..19409)
                     /gene="ftsZ"
                     /locus_tag="P5014_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010898709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HRY56584.1"
                     /translation="MMQVKPEVENFAKIKVLGIGGAGGNVINSMIESGQITGVEFIAI
                     NTDAQDLSVNKAVVKVPIGQEITNGLGSGANPEIGRKAAEESVDLIKEHLDGADMIFI
                     TAGMGGGTGTGASPVVARIAKEMGALTIGVVTKPFPFEGALRMKNAEIGISELRDEVD
                     ALITIPNQRLLEVADENMSIIEAFKLSDSVLNQGVQGISDLIVMPGLINVDFADVKTI
                     MKDAGTALMGIGIGTGENRAELAAKAAISSPLLEQSVEDAKGILFNVIGGSDLTMKEV
                     DKAANIIRNIANDEANIIFGTTIDPRISGQVKITVIATGFDVKQEDLGYGFVERNLPK
                     PAPAEEQYIKKEEPKSIPEKPKDMFKPEDFEGEFESKYDIPAFLRGK"
     gene            complement(19450..20703)
                     /gene="ftsA"
                     /locus_tag="P5014_00285"
     CDS             complement(19450..20703)
                     /gene="ftsA"
                     /locus_tag="P5014_00285"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HRY56585.1"
                     /translation="MSREKIITGIDIGSTKITTVVASVSDNKVSVIGVSGNIPSKGIN
                     KGNVVDIDSSVEAISQSIEKAERMAGVSISNAFVTLNGSHIENLNSHGVVAVSPNETS
                     EITKEDVSRVIEAAQAVSLPSTREIVHVIPRDFVVNSQEGIKDPVGMSGVRLEVETNV
                     IHGSATAMKNIAKCVNKVAIDVDELVYAGLASAEAVLTDTEKELGTLLVDIGGGTTSV
                     VAFLDGSPIHSAVLPIGGKLITSDLAIGLRSRLEDAEKIKIRLSNEALISKTDIHDDG
                     EDFDVSEFDLEVERIPRIMLYKIVNARLEEIFRLIALEVTRSGLSGKLPAGVVITGGA
                     AMTAGTEKSAKVNLKMPVRIGVPKGVTGLIDEIKGPDSSSAVGAVLYGAKLFRGSNLL
                     SFDNKNGSIRKGASKILSKLKSLLP"
     gene            complement(20715..21608)
                     /locus_tag="P5014_00290"
     CDS             complement(20715..21608)
                     /locus_tag="P5014_00290"
                     /inference="COORDINATES: protein motif:HMM:NF020067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HRY56586.1"
                     /translation="MKLFKSQKKSKNIIPIVNKTSLRNSSQTRSYSNHSRESQILLNN
                     INDRKRKVINSRVVLRKKHGIFLKILFLLIVVGVVIYLFFKFDVPGYFKISKVTITGT
                     ERFVSTEDVRNIAERNAFGKYIFSIEEDSLSKIISKSFLGAKNVFVKKDYPNSLNIYI
                     EERIPVAFVFNSEGEFYLIDSEGFVLGMVTESLQDLPRINYEGSVVVGTFLDKDIIPI
                     SIEILKFADREDLSISSMSFTTNYAKLYLNTGPEIFMGYSKDIEKSLRTIKALLSNQN
                     NNEEVIKKIDLRYDKVIVLYD"
     gene            complement(21577..>21887)
                     /locus_tag="P5014_00295"
     CDS             complement(21577..>21887)
                     /locus_tag="P5014_00295"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRY56587.1"
                     /translation="VSRSGAHTMSEIIALQKPSLLIPIPWVSHNEQYENAKMVKDLGL
                     ACILNEKDLNPESLIENIKFCLSDINSMKVKDESALDFLKNDPTELITNEIIQISKKI
                     "
BASE COUNT         7222 a         4224 c         3243 g         7198 t
ORIGIN      
        1 tctcaaaata cggagttgta acttataaaa ccaatgttcc ttataaatac gaaatagaaa
       61 gtattgttga aaaaccagct atggaaaaag caccatcacg aatggcacaa tttggaagat
      121 ttgtgctttc atacgatgtt gtaatagaag cagaaaaaag acagcttggg aaaggaaacg
      181 aactgtggct tacagatatt ataaacaata ttgctcaaag aggagacaaa gttattgccc
      241 aaccaatcga cggggagtgg ttaactaccg gggatccttt aagattcatt cagactacac
      301 ttaaatttgc gatgaaaaga gatgatctta aaaaagactt aacagaattt attaaaaagg
      361 aaattattta aaaaatcaca atcacattat tgtgatatta tatttttatg acagcagcaa
      421 gttcagggat tcagcagaaa agaatatcag aaaaagaact aagagacaga cttagaaaaa
      481 ggatgttggg aatatggagt atcgttatta taggaatctt aagtcttttt ttatttgcac
      541 cacaggtagg ttctttattt ggatactttt caaaatatag aaatgatcca gggtatgtcc
      601 caactcctaa accaataccc ccggtattca tagatgcacc taaagctata aataaagagt
      661 cagtaactct taccggcaga gcattgccgg gatctttcat aaaaatattt gtcaacggtc
      721 ctgaaaaagg ttccacaacg gccgggaatg acggaatatt taccttcagc gatataaact
      781 taaatgttgg aacaaacaca atctttgcta aatcttacga tgataaagga aatgaaagcg
      841 aaagttcaga gtttttaatt attaactatg ataaggacgc tccaaaaata acaatagaaa
      901 aaccaaagga cggggataca gttaaaaact tagacaagag aatagagata gaaggaaaaa
      961 ttgacgaaaa agctacaatt acaataaacg aaaaaactgc aattcaaaag tcagacctat
     1021 ccttcagtta ctttatgtcc gcatccgaag gagatgtaga aattaaaatc gaagtagtcg
     1081 acattgcagg aaataaatcg actaaggaat taaaggttaa gtacgtgaag ggacaatagt
     1141 ttctgcgcta cccctccata gataaaacac cacaatagag ctcaacaaag ataagaaaag
     1201 cccggcataa atgtaagggt ttagatagaa cagatcaact ttatctctct cccctacctc
     1261 actaatcacc ataaatccaa attcatcttc gcttagtatt actttattta cgttaagatc
     1321 ccgccaccat ctggaatatc gaacagacgt tttaatttca ttatcaactt tgtaatcatc
     1381 aacctttgaa atacttaatt ttgtcgggtg attatccaat tttttaaaat atggtttttg
     1441 aagatcactt gtttcaacac tgtatatata cataagttca ttccaatcat tctttgcctg
     1501 taaatctaaa taatcatatt ttttatctaa aatagaataa gatttaaagt tgctaaaaat
     1561 atcagctgtc cctacttctg agataagaga cacttctttc tcatttaagg gttttgtact
     1621 gataatataa tctgacctat taacgatatc tttaaacaca ccctctttta ttaaatttct
     1681 ttctgaagat gggctgtaaa aatatttaaa attatttaga gtatacaggt ctatatttga
     1741 gattacgtgc ggatagagaa atgtatctga actatctata aacgcaaata caccgggcct
     1801 tagattttta atagtattat atggaggtac cttatttact tcggttttat ccatttttgc
     1861 aaaattaaag ctataagtat tgttaccaaa agttgatggt cctgttaata tttcagcaaa
     1921 aaccagtagc gaaatgcata caattccaaa tttatctaaa ttcttaaaat ttttaagaaa
     1981 tcttctgctc aacacgtaat tcttaaacat ctctaaccca taaaccgaca gaatatacac
     2041 tggggtaaaa ataaaaacca taaatgcagg cactattgtg attctattaa aaaaaggaag
     2101 tgaaaataag atatttgata aaggattata cgtatttatt ataaatccga aaattattga
     2161 acatatcaaa aatacaaata ttttttctat ttttcttttt gaaaatattg aaaagataat
     2221 gtacacgaca cttaagaaga aaaaagctat agcaataata cccggggtaa ataaagcgga
     2281 accgaatagc tgtctgatgg gaggaaaata agacaaaaaa tctatcatat caatcttatt
     2341 caatctccat ccagttacaa ctccggatat cctaccggta ctgtttctca ttagatctaa
     2401 gaagggtaat atttttatag aaaataggag catcgataat aaaggaatta aaatgtagtg
     2461 tcgtatatct cttataaaat ttttagacat aaataacaaa aacaataacc aaaaagccca
     2521 tatcgagata acaatattta aattcccttc aagagtaaag gccagagaaa tagacaacaa
     2581 cgccaaatcg tgtaacttac ggtctcttaa gtagattaaa gtgaagaaca ttatcaatgg
     2641 aatccattta tatgaatata aatactcaat tcctactcct ggtgataacc tcatagtaac
     2701 aaaaccatta aatgtaaaaa ctatcgctcc aaaaaatgag aggtaattat taaatttcaa
     2761 ttctttaaat aataataatg aaaaaagcga agttaaaagt aactgaaaat atataaacac
     2821 acctgcacct tcataaacat ttttaaaaat taaaaatata aagttgagtg gagcaaaata
     2881 agctgatatg ggatttgaaa aagcatcaaa accacctccg aatctactgt tccaaaaagg
     2941 aggttcaaaa taattaacta tagattcctt aaaaaagtga aagaagggaa tctggatgtt
     3001 attaaaatct tgagctgaca gagtctgact tgtaaatata agtttattta aaaatattaa
     3061 tatcaagaga gacatacaaa atgcaggaat aagcaacgac tttaaacttt tcttaactct
     3121 ggcaagtatt aaaacaagat aaatattcgg tacaacaagt ataaataaaa gtaataaccg
     3181 gttaagtatt tctataataa ttggtttcat attatattat taagcttgat aaattataaa
     3241 acacttaggt actgtaacct attatcaaag ataaaaagta taggaaatag gataaacatg
     3301 atttgaggat agaataaact atttataaat tgagattcaa ttaaaagact tattaagatg
     3361 ccgagtataa cgagccatcc taaatatctt tttttcaatg aatcaataat aggaaataaa
     3421 aacgcaacaa aataaatcaa aaaaccaaga atcccagtcg ttgcaaaaac taaaaggagg
     3481 ctggaatcag accccgcccc cgagtgtatc tcatcttgat ctggggttaa aaacccatag
     3541 ttctcttgtg cgtacctata agtgttaaaa ccgacacctg tccaaatgtt atcctttatt
     3601 attctaactg tgttgcccca ggatacgatt ctaagggaag cagagtcggc ggggtccgta
     3661 gtacctgaaa tccttgtttg aaccctagga atggcaaaat aagccataaa ggcagtaaaa
     3721 aaagctacta ataacaatac tttagatcta aaaaccccgt atgctaagac aacaattccc
     3781 atcatacccc aagcacttct tgaaaatgtg agtatcaaac caattaaaat aacaatcaag
     3841 caaaaatagg tataaaaacc agttttagaa ctttcctttg tacatttatc gggaaatatc
     3901 ctttctaaca aaacagctaa gcaaataaca aagtaggctc ctacaaaatt tgggtcaaaa
     3961 aaggttgagg ctaagcgatt tttatgggga tcccaaccaa ggctcgggtc taaaatagta
     4021 aaatcaggta aaagtatcag ttgaataaac cctgcaatgg caataaatat tccggataaa
     4081 ataaaggcat cttcaatcct ttttttgctt aataatcttt tgtccaccat gttgtataca
     4141 actacacccg acagcagata agaaaaatat cgtaataaat agaaaaatcc ggtcattatt
     4201 ttatctgtag aaaactctat tgaagatagt aaaagggaaa tacctgcggg taatataaaa
     4261 aaaacataca aataagcgta tctcggtatt ttaaaggact ttttcaatat tagaaaataa
     4321 caagttccga ataatgaaaa taaaataaca ctaatgtcaa aaagatatac actcactccc
     4381 ccgctttttg ataacaccga taactgattc aaacttaaca agaaaagggt tatataaaag
     4441 ctagctacga gcatatttca acctccaaga ttttcgggca aaaatgatca aaagtataag
     4501 aactataaac tctgaaagta ccgttatcca agaagccccg tagaaagaat attttggaat
     4561 gaatataaag ttcattatta tattaaatga aaagctaaca aaataaatcc atggaagata
     4621 tttttggtat cctaaggtaa caatcaacca cgatattgga gatgtaaggt aaaaaataaa
     4681 caaccctcct gaaagtatat ttaaaacacc aacagattga acaaattcct cgccgcctag
     4741 gattttcata gacaaggatg aaaaaacaac acctagcaaa cctattatca accccgatcc
     4801 tgtaagaaaa tacataaccc gtgcaaatgt ttttttcagt ttctgttccc cctcaaccat
     4861 atggccaact aaaaccgggt aaacagaatt cataaaaaat gtaggcacta ccagcataac
     4921 ctcaaaaacc ttgtaaggta gggcgtaaac agccacactt tcggtattat tcagaccgta
     4981 agatttaggt aatttcaaaa aagaaagcat cagagcatct tctttaaagc tcatttggct
     5041 gaacaaaaac attatcccaa tgggaagtgc cgataaaatg agatatttcc atattttttt
     5101 atcaagagaa aaatccggct ttatgcccat tttagctaaa taactcatac ttaaaataaa
     5161 ggttagcagc ccgcctatta cgtaagaaaa gttcgcccac gtaacaggaa gtttgaagta
     5221 tgaagaaatt aaaactaaga caaggatgag aatataacct ccgacataag ctatggttga
     5281 gtaatcatat cttaatttca cctgaaaaaa aatatttgtc gtagagaata aagattgagt
     5341 gataagtaaa aagagtccaa gtctaatacc gtttcttaga tcagatgtat acggaaaaaa
     5401 actgatacta aaggagatta ttaatattaa aaacagggaa aataagaaac gaattattaa
     5461 aatatttcct aaatattttg ccgctttaga ccaatcttca gtaatatctc ttgctgcaat
     5521 ggcgttaata ccaaaatcta caatcacaaa aaatagagcc ggccaagatt gcatcaaact
     5581 aaatgctccg tacccttctc taccgtaaga ccttgtaata attaccacag caagcattgt
     5641 gatagacatg ctgattaatt ttccaaatat ctgatataca gtattgctgg caattttagt
     5701 cgctgtgttt gattttaaat taagtttcat ttgctaccag ggcagttata aatatctaca
     5761 ttatccgtca ttaaagccgg tggttttcct ttcacttttt cgattttttt caaaacctcg
     5821 tattgaagat aaggaacttt gtctttatta atccaagaga aatggtcgaa tggagggtcg
     5881 tatctgatcg taaacggcat gactgccctc actctatcat ctttcaacca tacctcctca
     5941 aaagctattt tataatattc cgacacagtc tcaactttcg gaaaggaagg gttataatta
     6001 tcaccttcgg catgtgccca ccccgtttcg gttataaaaa ccggtagatc ttttttaact
     6061 cctaaatctt cttttaaata tttcaactca tcctcgtaag ctcggatgct ccacctcccc
     6121 ttatcgtaag gattgcctga aaaatttggt tgaggataag gatgtgaagc ccaaccatct
     6181 aatttatcaa atattcctgg tactttttca ttcatttttc tcataaactc aaaagcgtca
     6241 atattttgac cgtcggttgg agaactaatg tttaaagctc cgtttaacat ataaaaatca
     6301 gggttagttt ccttaaaggt tttaatagta aaatctaaaa cctcggcgta ttcctccgga
     6361 tcagcatcac ctacccaaaa cttatcatcg ttaggttcat tatatacact gatgtagcga
     6421 tactttatag gccagataaa ggaatttaaa aactctgcgg ctctcttggt atttttttga
     6481 taatgatctt tatcaaaatt ccataactga attacaggaa ttaatttctt actaattaat
     6541 tgatcaaaaa ccctccccca cttaccttca tcgtaatctt ttacattgta gggtattaaa
     6601 acgtaacccc actcccctcc ctttgagttg attaaatttt gcgcaatctc aaaaaaatct
     6661 ttttcttcag catagatata aagcccgaac ttattatttt ctttccatag cggatcatct
     6721 ccagaataat agtaatattc gatattttgt atagcagtac actcttcttc ctgttcccac
     6781 cagtcttcag acttaaaaac aaaatccatt tcaatattct caatgtcttt ttctaacttc
     6841 ttttgctttg atgtaacaat gtttttataa aaaaggtaag acaatacacc aaatagtatt
     6901 agcgacaaca agaaaaaaga ccaaaatttt cgcttagtct tagatatatc catatttatg
     6961 caattttaac attttattaa agaccttagt gtaattatcg tgtaaaatta acccgaatga
     7021 aaacaaaaaa aattcttatt atttcgatat ttgtcatttc agccttgtta tttacggcct
     7081 cgatgtctgt aaaaaaaaca ggtacaccaa aaaatatcag aagcagtaaa gttgtctctc
     7141 ctttttcaag taacataaac gtactttcaa gcttttcaaa aatatcagag aagccaagga
     7201 aattaattta cgggtacctg ccttactgga atattaccaa aatagagtat cttcagctag
     7261 ataaactaac tgatatagct tattttggtt tatatttaaa tgaatacgga gatttcatga
     7321 ctacaactat cggggaagat ggaacaacca taatcgaacc cgcctacaat aattggaaaa
     7381 acagtgatga gcttaattta ctaataactc actgtaagga aaataatgtc aggtttgcac
     7441 ttactataat agctcacgac gatgcctcta atgacgagtt tcttaactgc agggagtgtt
     7501 gggataattt agtaaaaaat ttaagaacag agttagatag aaaaggtata agggatgtta
     7561 atttgaattt tgaacacgct gaaggaacaa aggaaggtat tgataaaaag ttttctgagt
     7621 ttaccaaata tttaaacggt gaacttgata aaatttatgg aaattcttat gttgttgttt
     7681 cggctttcgg agattccgca acacaaaaca gagtcagctc tgatttggaa aacttgagcc
     7741 gggcagcaga tggaattttt ataatggctt atgattacca ccaacctact tcagaagaaa
     7801 taggtccggt atcacctatt gaaggaccgg gaattgatat aaaagaaaca attaatgact
     7861 ttttgactaa agtacctcct aataaaataa ttcttggagt accttattat ggatataact
     7921 ggctggtaga agatgaatct caatactcaa aaagaaaaga cgggtcagat gaaaacgggt
     7981 attcacaatc tcaatcatat gaagacatac ttggaaccat acttgagcta aatcctgttt
     8041 taaaatggaa tgaggaaggt aaatcaccat attttacata tgtaagtccc gaaacagggc
     8101 aacttagaac cacatactat gaaaatgcaa aatcattgaa atacaaatac gatattgtta
     8161 atgaatacaa ccttggaggt gtcggaatgt gggcactcgg atatgacggg ggttacaccg
     8221 agctctggaa tttgctctac gattacttta ttaaataata aaaaaaccaa gcacaaacaa
     8281 ggttacccaa attacaaaaa cgtaaatata ctgcacaggc attggcccaa tccaatcctc
     8341 gtgccaagag atatgttttt ctttgaactt ttctaatccg aaatgtgggt taaaaacaaa
     8401 ccacaaaaaa tcttccagca tcgctattaa agtgtaacca gacaaaatta ttacctcggt
     8461 tcttaagtcc cacttcataa ataaaaacgg tatatgaacc aagaatagaa tcattgtgaa
     8521 aagatatata tgataacctg taataggacc acggaagccg gggagcgcgt ctaaaaactt
     8581 gaagcttttt ttccatgttg gtaatttttt tgcccacccg tgctttcctt cggtttgtat
     8641 ttcaaacaag gcaagcagta tcgctaccaa taaacaaaat ataaaatatt ttatataaat
     8701 cataataata ttatattcct taatttcttg gtgggcccgt caggattcgg tcacctgttc
     8761 gcatctgctg atgcgccagt cgaccccgcc tcaccgccgt cgcggttgcg gcattcgaat
     8821 cctttcttta gctcaaatcg ttaattggtg ggcccgtcag gattcgaacc tgagaccttt
     8881 gcaatgtgaa tgcaacgctc taaccgactg agctacgagc ccacgatatt atctatctaa
     8941 cattttacta caccttgtac tttttaccca tctggaatac gattagataa ttatgtttga
     9001 taaaaacgtt aaaaaagtaa tagtggaata ttttcagatt atttttatat tcctaatagt
     9061 ctttgcgatt atctactttt ttgttgggca gctttttgag gttgtcggag attctatgat
     9121 tccaaacttt cgagataaag aacaactgat agctgaaaaa atctctataa atattaataa
     9181 tacagagcga ggggacgtta taatttttaa aaatataaat gaaaacaaca ggcttttaat
     9241 aaaaagaatt attggattac ctggagagaa tataaaaatt caggatggaa aagtgtacat
     9301 taacggggta gagttatcag aaaattatct agaagaaggt actgttacag aaggtaaaag
     9361 aatactagag gaagggcctg aatataaaat acctaatgat tcgtacgttg ttatgggtga
     9421 caacagagaa caaagcaatg actcgagaga gtggggatac gtaaaaaagg aaaatattgt
     9481 tggaagagtt gttttagttt attaccctct tcaaaacttc aggtttgtaa aataaataaa
     9541 ataaaaaagg gtagatttat tttttggttt cttttttctc taacacactg actttcttct
     9601 tgctttttgt tttccatgaa aaatccacaa caccatctgt tttagaatat atagtaaaat
     9661 ctttacccat actcacatta tctcctggaa ctatttctct gcctctttgt cttaatatga
     9721 tctgtccggt tttaacagca gaacctccgt aaaccttaac accaagtctt ttaccgctga
     9781 cgttaccacc ttgaactgct ttggaaccac ctgcttttgt atgtgccatt ttattttgta
     9841 cctttcaact ttttatctac aggttttttt gttggagttt tagctggagt agtttttttt
     9901 ggtttctcac ttgtaacagg tttctttgtt tcaacagctt ttgcaactgt tttcttttct
     9961 ttaatttcag gtttttcgga tactttttct gcttttactt ccttttcttc tttggcagac
    10021 ttaccgtcac caattttttc aatttttact atactcatag gttgtctgtg acctctaact
    10081 ctttcgtaac gagacttctt tttaaatctt gctactctta ctttcttacc gagcttctct
    10141 tcaactacct ctgctcttac agaaatatcc tttatttcag gctcgcctat aatagttttt
    10201 tggccatcgg tataaaacaa aacatcaacc ctaagaggtt tttcttgtct ttccaactca
    10261 acaacatcgc cttcttggac gagaaattgt ttagaaccta attttattac tgcatatttt
    10321 tccatagtaa acataaggat attacactaa tattagagtt tgagcaatac cgtaactaaa
    10381 gctcggaact cgttccaaca gttattagga gatcataatc actgtcttca gaaatattat
    10441 ccttaacctc aatttcagga aacttagttt ttatatcttc tttgactaaa ttctcaaact
    10501 ctttttggac tgtggactta aactgcaata ccgtaggctg gacctttgaa tcggctgtat
    10561 ctatcgttaa aacggtgtaa cccaagcctt cgagatattc ctttgttctg gcagctaaac
    10621 ccccgacccc cgctccgttt tcaactctta tctttaaatt acctcttttt acttcaacat
    10681 taggatcttt ttcaggattt tcattttcgg taccttgctg ggattgctct tcctgtacag
    10741 gttgttcttg agaggtggtt tcgctcaaga cctgagtctc ctgggatgtg ttattttgcg
    10801 ccaactgttc gttccccgct tttttatttc ccaaagaagt aattccaaaa tatgctccta
    10861 ccaaaagaag caacgctccg attacagaac cggtaaccag aagtgcggag ccttttcttt
    10921 caacgacagg gagtggcagc atgtttaata catttatctg cccacccaat atcgtggggt
    10981 ctgaatcaag caaaaagttt acgatgttat taatttcaaa accttcagga agattgaaat
    11041 ttccattaaa tttcggtgat tcaatcttct taatctcata gttaggcata gtaaaagaat
    11101 cacaattaaa agtgaagatg cattttatcg ggctggatct tttataaaaa gataattcct
    11161 ttaccaggga tgttaattct tcggaggttt tttctttttt gtaagtacct gcaaagaaaa
    11221 tgccatttaa ttcagaaagt gctataactt gatcgttgtc aaccttagaa acaaatattg
    11281 caggatcatt aacattttca tacttgggaa aagccagtac ccagggaata atactcttta
    11341 atcctatatt caaagcgttt gatgtctcca aataattatc cattacttct ttcctaatac
    11401 ctacgaattg ataaacaaac ggagccattt ttttatatga aaaataaagt gtatccaact
    11461 ttatatcagg atcctttgcc tttatctcgg aaattatctg ctcaccagtt tcctgtccgt
    11521 tttttgaaac agtgacgtat cttaaaaaaa tatcttgggg ttccataaca aagttcaaac
    11581 ccaatttatt ctttgatagt actgaaactt gagaaattga tttttcaagt aaatttgaaa
    11641 aaccttttgg atcaattatt cttgtgtcat cgactaattc cttaggaaca tttaacatcg
    11701 ctgtttttaa ttgagcttct ttgtcaattg ttgagatttt tagtatattt ttattgatag
    11761 atacaactaa gtcctgcata tatttaatat aacataatta taacttttct accttgattt
    11821 cttcaccggg aattatttct tttgccaata gtttattttt aatttcgtct ccgtaaagag
    11881 agattatctc actcatatcc gaactgtctt ggtaagttaa aacaatttca tcggaaatct
    11941 gcaaccccat ttcctttcta atatcttgga cgtttcttat gaactctctt gcctttcctt
    12001 cttttagtaa atcctctgtt atttcaatat taagtttaat tgagttcgta agatcggttt
    12061 cccacatcgg tttctcatca agagaatcta ctacttcaat attttttata tttgtttctt
    12121 ctaatatcaa cttcttatat tcttccggga catcattttc ttttaaaatt gctgaagaac
    12181 ctagcacttg tctcacagga atagattttt caactcttac agacaatacc tgggatacga
    12241 tatctctagt ttttttcata tttatcagta tttcctgaga ttcgtccgtt aaatcattat
    12301 taaaaacggg atagtcacaa agatggactg atttttcatc taaattttct tcttgaagtg
    12361 ttctgtatat attttccgat ataaaaggaa tcaaaggagc cgaagctttt gaaaattcta
    12421 aaagaactgt atataaagtt gataaagctt cgttatcccc tgaagaaatc ctatctctag
    12481 atctgcgaac gtaccatctt gataaatcat caataaaacc ttctaaagat ttcaccgccg
    12541 gaggaacatt gtacagtttt aaaaatttat ctatatcgga tattgtttga tttagtctta
    12601 tgataatcca tttatcaagt acgttactag atattacttt attctcagag ctccagttat
    12661 tactttgagc atacattaag aaataaactg ctgaattcca aagtggatta agaatatttc
    12721 ttgatttgtt ttttaactca atttcatcga agttagtgtt ttcacctgac atgagggagg
    12781 aacccattaa gtaaagtctt aaagcatctc ctccgtattt ttccattatt cccctcggat
    12841 ccgggtaatt tttatatgtc ttactcattt ttctaccgtc tgtaccagcc attactcccg
    12901 tcactataac attattaaaa catctggaat taaaaataag tgttgaaata atatgcatcc
    12961 tctggaacca cgctcttacc tgccccttat actcgacgat ataatctccg gggtaatttt
    13021 tctcaaattt ttctctattt tcaaaaggat agtgaagctg tgcaaaagac attgaccccg
    13081 cctccatcca agaatccaaa acttcaggtc ttcttctgta ttctttaccg tttttattta
    13141 taactatttc gtcaatatag gggcggtgta aatctttaac tttttgtcct gaagcttcct
    13201 caatttcttt tattgaagaa aaaactaacc tatccccatc ttttgattcc catactggca
    13261 tcggtgttgc ccaaaacctg tttctggaaa tacaccaatc aggtgcctgc tcgatagttt
    13321 tttcaaaaat accatgcttc acgtattcgg gtacccaatt aatatcttca ttattttcta
    13381 ttaactcatc ttttattttt tgaatatcaa taaaccaaga gttttgggct ttttgaacaa
    13441 gaagagtatt acatctatcg tgatagggaa ctctgtgtac tgctttttcg tttttaaata
    13501 tcacaccttt ttcaactaaa tcattcaata ttattgcatt agcttttagg taaaacatcc
    13561 cttcataagg atttgaatct tgatttcctg gtaacatctt accttcgtca tcaagagtca
    13621 tcatcaaagt taagttgaaa tgtttaccca tttccgtatc tacctcgcca aaaccgggag
    13681 cgctgtgaac tatccccgta ccctcctcca ttgttaccat gccttcatag gaataaacct
    13741 taaactcccc ttcttttgag gcgtaaaaag taaacagggg ctcgtactcg agccctataa
    13801 tttcttttcc taaaaactct tcttttattt cataattttt atctgaaaaa acaaaatcta
    13861 atcttttttt ggctaaaata taagtattgt tttcaaattg tacggaaacg tatgtttcct
    13921 tgtcgtccac cactaaagct cggtttgaag ggagcgtcca cggagtagtc gtccaagcca
    13981 aaagataata atctttaaaa tctccttccg ttataactct aaatctcact gttatggaaa
    14041 gatcattttc ttctctataa gaattatcca ttgcaatctc aaaatctgaa acaggtgtac
    14101 cgcaagtagg gcaaaataat gaagttctgg taccttcata aatcaaaccc ttttcataaa
    14161 tttgtttaaa agcccacata acgctttcca tatacttttg gtcagttgtt ctataggcat
    14221 ttttgaaatc aacccatctt ccgattttat ctacatacca ctcccattct gctgagattc
    14281 ttgaagtgta ttcgtaacaa gcttgagtaa atttctctaa tccataactt tcaatatccc
    14341 ttctattttc aattcccagc tcctcctgta ccctattttc aacagttaaa ccatgagcat
    14401 cccaacccca aaccctctca actctttttc ctttcatagt ccaaaatcta ggaataacat
    14461 cttttgctat tgaaacaagt aaatgtccgt aatgaggaag acctgaaata aaaggaggac
    14521 catcataaaa aacatacaaa ttatcttcag gatttttctc tacagatttc ttaaaaatat
    14581 tgtttttttt ccaataagat aatatctcat cttccatttc tggtatttgc tttttatcag
    14641 gtaatttaaa cgacataata aaatatatta cattaaatta gaccattaac caattattcg
    14701 gttattaaaa actcactacc agtgtgttca ttaaaataaa catctgtctt tttggcgtta
    14761 ataaataata ttgccattaa agcaaacaac gacataaata tgataaacag gaagaaataa
    14821 tactttttca cgactcttta ttgttccaga ttatatcctt aaagccaagc tttttatttg
    14881 agtatctcgc cattacgaat aagaggtcag acagcctgtt taaatattta ccaataaaca
    14941 atttattctt atcttttttt aaatattgaa aaaccattct ttcaagtttt ctgcaagtta
    15001 cgcgtgataa atgtaaaaag ctgctctccc tacatcctcc tggaagaata aaactctcaa
    15061 gagggtcatt tttattatta aaataatcaa ttacctcttc taactcttta attctgtttt
    15121 cccacttttc aacagactct tttgatattc tttcatttga tgaaataact gatcctaatg
    15181 aaaataagtc tttctgaact tcttttgcaa ctttcctcac atcatctaat ctggctgagt
    15241 gaagaaagcc caaggaaact tctaactcat cgaaagttcc caaaacatca aaaataaatt
    15301 cggttttaga aattttttta aacatatttg ataaggaggt aaatcctaaa tccccttctt
    15361 ttgtatatat tttatacata tgaaatagga cagaatggca tcaatatacc ctaaaatagg
    15421 ctgcagtcaa tcagaatatt ctaattaacc aagtatatta agaactaaca aacagagtat
    15481 ccacaaaggt agcatttagc acaaccctct tctttgatga aggaataatt tccgcaatca
    15541 gggcatacat cgatatttcc ggttacagga tttaaactta cctcgctggc atcactacgt
    15601 atgtttaagt attctgtgtt tatttttgta cttataaaat tatccccgtt tccatttgac
    15661 tgatttttta acaaagaata cccaagttct tcagacaata cttttgccac agcatcagga
    15721 atggaactaa ctttattttt tccaaaacct accgatctac taccgccgat acctcctaat
    15781 tgagaaataa tctcagttac agcattttct gatgccccgg gaactctaag ccaaccacta
    15841 actaatctac ctaaaccttc agataatgca gaaacatcag tacctgcctt tccaacatta
    15901 ataaatacct caaaaggcct gccgttagaa tccttattta ttgtaataaa agcttttcct
    15961 tgaggtgttt ttacacagta agttgaacca tttaatctat actcacgttc taatttaaca
    16021 tcagagggtt tttctacgat taatttttct tcattatttg taatttcttt tgatacatct
    16081 ttattggcat caatttttcc ttcttccacc ggtttttcac catcggattt accaccttcg
    16141 gttgttgatt cttttttttc ttgtgtaact aatacctctt cttgtctact gcccgaaaca
    16201 tatactgtta aacctttaca acccaatttc caacccatca tatatacttt ttcgacatcc
    16261 tcgggaactg ctgttggggg taaattacat gtcttagaaa ttgagttatc tacaaatctc
    16321 tgcaaagaag cttgcatata aatatgttct tctggcgtaa tatcacctga taccacaaat
    16381 gtatgtcgta gatcatcggg taaatctttt atactttgac acgaacctac tcgaattatc
    16441 tcatcaataa tctctcttct cttaacatca ctcatgtcta tatcgtctaa cgctttttga
    16501 aatgacgggc tgacataggt cagagtcatt ttctccaagt cacctgctgc ttggtagaca
    16561 tttctaagat aagcaagtgc aaaaactggc tcacacccat aaccctctac tccagcaaca
    16621 gtagaaatgg tacctgttgg ggctaccgtt gtaacagcag catttcttaa accatacagt
    16681 cttattccgt tcgttatttc actccaatcg atggcgggcc tttcccagcc aaacgaaaaa
    16741 ctctttaatg gtgtgggagg agtccaaact aaattagatg gattgaagat actgtctttg
    16801 atagctggga aaggccctct ctcttttgcc aactctatag acgctttcat agcgtgaaag
    16861 cgaataaatt ccatcacctg ccctgctaaa tcctgtgcct cctcgctacc gtaccttact
    16921 cccaaaacat aaaacaaatc agctaaaccc ataattccta aaccaattct tctattttga
    16981 tgggctgcct cttttaattg aggtactgcc ggaacatatt tatttgcact tactacgtta
    17041 tctaaaaacc ttgtggaaag tattacagtt tttcttaatt cttcccaatc tattttattg
    17101 tcacttgtaa tatgctcgga taaatttatt gatcccaagc aacagttttc ataagggcca
    17161 agccactgct ccccgcatgg atttgtagct tccaattcat aaaggctggg aacagggtta
    17221 tctttattag ccgtatcaat aaatagaact ccgggttctc cgtttttata tgcattttca
    17281 actattagat caaatatctc tctggctttc ggagtttcca caatctcttt tgtatgagga
    17341 tcaaaaattt catatgtttc atctttttca acagcttcta aaaatttatc tgttaaagca
    17401 acggatatat taaaatttga tagttttcct tcagtagttt tagctgtaat aaactctctt
    17461 atatccggat gatctactct caatattccc atatttgcgc cttttcttac cccgccttga
    17521 gctattacac caaaagcacc gttatatgcc tccataaatc ccacaggccc agtggacaaa
    17581 ccgttgctcg ttgaaattct cgaccccttg gctcttaatc ttgaaaatga aaagccattt
    17641 ccgccaccgg attgctgaat cagtgctgcg tttcttaatg tctgaaaaat tccatctctg
    17701 ctttttccaa ggtcgtcttc caccggcaat acaaaacaag cagccaattg tccgatggga
    17761 gttcccgctc cagtaaatgt aggagagtta ggaaaaaatt ttttggtgga aattagatta
    17821 taaaactcaa tggaggtttt ttcaggattt ctataaggct catccggttc ggcgatcgcg
    17881 tttgccactc tttgaaacat atcctcgggg gtttcttcga tctcacctaa tttatttctc
    17941 cttaaatatc ttttttctaa aatagttctt ccgttttctg aaaagtctat tttttcattt
    18001 ttctgtaaat ccgactcatc attaaaataa agatacgaat tcatttttat tattatttag
    18061 tgagttaaaa acctctgctg ccctaaatac acaaaggcca acaactcaca agaaatttta
    18121 tacttttatc ttcaaaagga atttttactg cctatcgtta atataaccaa aattttatta
    18181 aaaatccaag cagtgaatat tagacaattt gataatattt agtgtaatta agactggaaa
    18241 cgtagttttt ataagtagga tttctattta cctcttaaaa atgccggtat gtcgtattta
    18301 ctttcaaact caccttcaaa atcttcgggc ttgaacatat cttttggctt ttccggaatt
    18361 gatttcggct cctctttttt aatatattgt tcttccgcag gagcaggctt aggcaaattt
    18421 ctttccacaa atccgtaccc caaatcttcc tgttttacat caaaaccagt tgcaataact
    18481 gtaattttaa cctgaccgga aattcttgga tcaatagttg ttccaaatat aatattcgct
    18541 tcgtcattgg caatatttct aataatatta gcggccttat caacctcttt cattgttaag
    18601 tcagaaccac cgataacatt gaataaaata ccttttgcgt cttcgactga ttgttctaat
    18661 aaaggtgatg aaattgcagc ttttgcagca agttccgctc tattttcccc ggtacctata
    18721 cctatgccca taagtgctgt accggcatct ttcataatag ttttaacatc cgcaaaatcc
    18781 acatttatta atcccggcat aactattaaa tctgaaatac cctgaactcc ctgattcaaa
    18841 acgctatcgg aaagtttgaa cgcttcaata atagacatat tttcatctgc aacttccaat
    18901 aacctttgat tgggaatagt aattaaagca tcaacctcat ccctaagttc agatatacct
    18961 atttctgcat ttttcattct caaagcaccc tcaaaaggaa atggttttgt aactacacca
    19021 attgtaaggg cacccatctc tttagcaatt cttgctacaa ccggcgaggc accggtacct
    19081 gtaccgcccc ccatacctgc tgtaataaat atcatgtcgg ctccgtcaag atgctctttt
    19141 attaaatcaa cagattcctc agctgctttc ctgccaattt ccgggtttgc tccagaaccc
    19201 aaaccatttg ttatttcctg accaataggt actttaacca ccgccttgtt aactgataaa
    19261 tcttgggcat cggtattaat tgcaataaat tcaacaccgg taatctggcc ggattcaatc
    19321 attgaattta ttacgtttcc tccagccccg ccaataccta aaacttttat ttttgcgaaa
    19381 ttttcaactt caggctttac ttgcattatt tattttttgg gcagattaat taattttaaa
    19441 aaaatacttc tagggcagaa gcgatttaag ttttgatagt atcttagaag cgcctttcct
    19501 tatactaccg tttttattat caaatgacaa tagattactg cctctaaaga gtttagcacc
    19561 gtacaacaca gctccgacag cggaagaaga atcgggtcct tttatctcat ctattaaacc
    19621 ggttactccc ttaggaacac ctattctaac aggcatcttt aaatttactt ttgcactctt
    19681 ttctgtacct gcggtcatag cggctccgcc ggttatcacc actcccgctg ggagtttacc
    19741 acttaacccc gatcttgtta cttctaaagc aatgagtctg aatatttctt ctaatctcgc
    19801 gtttacaatc ttataaagca ttattcttgg aattctttcg acctctaaat caaattctga
    19861 cacgtcaaaa tcctcgccat catcatgaat atctgttttg gaaattaaag cctcgttgct
    19921 aagtcttatt tttatttttt cagcgtcttc aagtcttgac ctcaaaccta ttgccaaatc
    19981 actagtgatt aatttcccac ctatcggcag aaccgccgaa tgaatgggac ttccgtccaa
    20041 aaatgcgacc accgacgttg taccaccacc aatgtccact aataaagtac ctaactcttt
    20101 ttcagtatct gttaatacgg cttctgcgga tgctaaacca gcatagacaa gttcgtcaac
    20161 atcaatcgca accttattta cacacttagc aatatttttc attgcagttg cagaaccgtg
    20221 tattacattt gtctcaacct cgagcctaac gcctgacatt ccgacaggat cctttatacc
    20281 ttcctgtgag ttaactacga aatctcttgg gataacatga actatttctc ttgtggatgg
    20341 caaagatact gcctgggcag cttcaattac tctagaaaca tcttctttag ttatttctga
    20401 agtttcattt ggggaaactg caacaacacc gtgtgaattt agattttcaa tgtgacttcc
    20461 attcaaagtt acaaaagcat ttgaaatcga tacccccgcc attctttcag ctttttctat
    20521 gctttgtgat atggcctcaa ccgagctgtc gatgtcaaca acattacctt tgtttatacc
    20581 ttttgacggt atgttcccgg aaacacctat tactgaaacc ttgttatctg acacagatgc
    20641 aacaacagtt gtaattttgg tagatccgat atcaattcct gtaattattt tctccctaga
    20701 catagagttt taatttaatc atataatact attactttat cataccgtaa atcaattttt
    20761 tttataacct cctcattatt attttgattg ctcaacaaag ctttaattgt tctcaatgat
    20821 ttttcaatat ctttactgta acccataaaa atttccggtc cggtgttaag atataattta
    20881 gcatagtttg tagtaaaact catactactg atacttagat cttccctatc tgcaaatttt
    20941 aaaatctcaa tacttatagg aatgatatct ttatccaaaa atgtccctac caccaccgat
    21001 ccttcgtaat ttattcttgg taagtcctgt agactctcgg taaccattcc taatacgaaa
    21061 ccttcagaat caataagata gaactcccct tcactattaa atacaaaagc cacgggaata
    21121 cgttcttcta tataaatatt taaagaattt gggtaatctt ttttaacaaa aacatttttt
    21181 gcacctaaaa aacttttaga tattattttg gacaaactgt cttcttcaat tgaaaatata
    21241 tatttaccaa aagcatttct ttcagcgata tttcgcacat cttccgtgct tacaaatctc
    21301 tctgtaccgg ttattgtaac ttttgaaatc ttaaaataac cgggtacatc aaatttaaaa
    21361 aataaataaa taactactcc gactactata agtaaaaata aaatctttag aaaaatacca
    21421 tgtttttttc tcaaaactac tctggagttt atcacttttc tttttcgatc attaatatta
    21481 ttaagcaata tctgactctc tctggaatga tttgagtagg aacgagtttg agaagaattt
    21541 ctcaaactag ttttgttaac tataggaatt atattcttag attttttttg agatttgaat
    21601 aatttcatta gttattaatt ccgtagggtc gttttttaag aaatccaacg ctgactcatc
    21661 cttgactttc atcgaattaa tatcggataa acaaaacttt atattctcaa taagactttc
    21721 aggattaaga tctttttcat ttaatataca agctaaacct aaatccttta ccattttcgc
    21781 attttcatat tgctcgttgt gagataccca aggaataggt attaacaagg aaggtttttg
    21841 taatgctata atctccgaca tagtgtgagc acctgatctt gaaacta
//
LOCUS       DAOTFA010000003        14686 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate AMR_MDS_3115
            NODE_2824_length_14686_cov_8.095551, whole genome shotgun sequence.
ACCESSION   DAOTFA010000003 DAOTFA010000000
VERSION     DAOTFA010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868139
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (wastewater metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 14686)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14686)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:36:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 536
            CDSs (total)                      :: 521
            Genes (coding)                    :: 520
            CDSs (with protein)               :: 520
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14686
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2824_length_14686_cov_8.095551"
                     /isolate="AMR_MDS_3115"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..699
                     /locus_tag="P5014_00300"
     CDS             <1..699
                     /locus_tag="P5014_00300"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HRY56588.1"
                     /translation="LSFDTTLHLGTLFALIVVFWKDILSIIKSLFLDIKKHKKSFNHY
                     SSKSWLAFKIFIASIPVGLAGLFFGDFLENKFRGIWFVIIFLLVGTLIMYIAEKRFKK
                     RLIVKDEISVGKSFRVGIFQALALLPGISRSGSTISGGMFFGLSRKEAARFSFLLSIP
                     AVLAAGVSQIISSLDYLNMAEIAPMMVGFFSSFLVGIIAINFMMKFVKNNKLYPFIFY
                     RLGLAVFLIILFYI"
     gene            725..3277
                     /gene="secA"
                     /locus_tag="P5014_00305"
     CDS             725..3277
                     /gene="secA"
                     /locus_tag="P5014_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013782216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HRY56589.1"
                     /translation="MFGSLFDTNEKQLNKIWPVVEEVNSLEEGVKKLSKEEIQGKTKK
                     WKEELRKFDEKEIDNYLNKILPEAYALVRESATRTVNMRHYDVQILAGIVLHQGKIAE
                     QKTGEGKTLTATLPIYLNSLGGKGVHLVTPNDYLSRHGAGWMGALYDYLGVSVGVIMQ
                     ERAFKYDSSFQGTEFEDSYSVHLREVTRQEAYQCDIVYGTNHEFGFDYLRDNMVRSLS
                     DMVQTNPRGDWGVHNFAIVDEVDSILIDMARTPLIISTADAKPSERYHQANGIVKSLI
                     KDTDYEVEEKYKNVTLTDLGIRRVERLLGIENLYEQDFEMVHLMEQALSANTLYEKDK
                     DYVVKNGAVVIVDQFTGRLLPSNRFSHGLHQAIEAKEGVTIQQESKTLAEISYQNYFR
                     MYGKLAGMTGTAATESEEFFKIYKLEVVIIPTNKKCIRKDNNDVVYKTEAGKFKAVAD
                     EIEKRYKKGQPVLVGTTSVDKSELLHHFLQRRNIPHEILNAKNHEREALIIAQAGREG
                     SVTISTNMAGRGVDIILGGDPVDLDMQKKVVEAGGLCVIGTERHESRRIDNQLRGRSG
                     RQGDPGESKFFVSLQDDLMRIFGGESVSRIMDRFGLDENIPLEAGIVSKSIENAQKRV
                     EGFNFDRRKQLVEMDDIMNTHREVIYKLRRRILELGGGVEDYKDWLVEKFSEFSSLSR
                     EFWDEREKEFGKETWFQIVSGFGCPVIDFMWMDHLVDMDQLREGIGLRGYAQRDPMVE
                     YKREGHERFEILVSKVYSMVGERLSSISKDAVPEKSSHSDVSRKLTYHKSELETGVSE
                     EGMEMSERKVVDDSGREMKVEKIESGREKVGRNDPCPCGSGKKFKNCHGKNI"
     gene            complement(3556..5223)
                     /locus_tag="P5014_00310"
     CDS             complement(3556..5223)
                     /locus_tag="P5014_00310"
                     /inference="COORDINATES: protein motif:HMM:NF024816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY56590.1"
                     /translation="MKKDKEIKSVDVVNNSDLTFYNFKTFKKFIKENKLFIVVITIFT
                     LLIYANIITGDFVNLDDYSKAFTSPEFHDFGLALKSFDAFTIVMSVVVQTFGINSSVF
                     HIVSIMFHMLNGILVFILLNILFGKKISIIGTLLFIAHPANTEAISWHAGVWYAGRAS
                     IIIPTLIFFALFKKTDNKIYLLISTLIYVFSLIFYRGGGWILVTPFLLVLMDQFIIEK
                     KIKFSNIKQYLPYLLTSLAFAIALLPGYIKERVQGLETLYYVDTATATPLINRIPYTI
                     YMEYTTLFYPKTLSIYHEGKYITPAEYTFMVFLTFAVIFAIFYFMKKDRRISGLLLMI
                     IFGVLPSFSPVIVAWIAAERYLYIPSIFSSAIIGIGLIYFENKINEKNKKQGKVKKGV
                     EKNNFVLYASIIIISLYSLRTVLRNNDLRNSKNLWIASKKTAPYSYRVYNNMGDVLAN
                     EGDLEGALENFKRSYALKPDYADAVHNIGHIYMVQGNIPQAIRYLEQSLQMNPRLYPS
                     AYKLGIIYMEEKDFENAKKYFEACLQYEPTSADCNAGLQTANQMLAQ"
     gene            5300..5845
                     /gene="lepB"
                     /locus_tag="P5014_00315"
     CDS             5300..5845
                     /gene="lepB"
                     /locus_tag="P5014_00315"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HRY56591.1"
                     /translation="MKIKSFIGEIIETVLLSAVVILMIYHFIASVEVVWGPSMEPNFH
                     SGERILVDRVTTKFSELKRGDVVVFYPPNDDSKHYIKRVVGIPGDVFKIINCNVVISR
                     DGKHYVLSENYLSEGVCTEAGSFIKEGRSIKLTDNQYVLLGDNRPQSLDSRNIGVIDK
                     SRLVGRVVFRFWPISKIGFIK"
     gene            6033..9368
                     /locus_tag="P5014_00320"
     CDS             6033..9368
                     /locus_tag="P5014_00320"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF001616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0032549 - ribonucleoside binding [Evidence
                     IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HRY56592.1"
                     /translation="MRETFLKRKIALPLPNLVGVQLESFDWLKRVGLKEVLDELGTIE
                     DNSGRGWVLTLSDPRVDKENITIEEAKRTGRTYDAPWYVQASIEDPISKKKKVNEIYM
                     GDIPLMTDQGTFVINGVERIIISQLIRSEGVLFGGENSPVTGQFLAAAKVLPKSGVWL
                     EFETSRTGVITLRIDKKRKITATTLLRVFGLESDDDIRQAFVGVETNPEINYTEATLA
                     KDPAEGFDDACLEIYKKMRPGEPLVIDNAKALVHAMFFNKRRYSLGSIGRFKLNQTLG
                     LNFPNDPKHKLIQLEDLIKILSRIIELNNGIGDPSDVDFLGNRRVKSMGELLQWQMRV
                     GFLRMEKNIKERMSLAPRDRLPEPSTLIAPRAIAAAVHSFFATGQLSQLHDQQNPLTA
                     LDHLRRLSVLGPGGLSKDRASFSVRDVHNSYFGRICPARTPEGPNVGLINYMSMYARV
                     NQYGFLETPYIKLEKDSKGNVKITDEVVYLAAYDEEHTNITDQSVEIDEKGYILDEHV
                     PIRKGGEFFLGDRATAEYIEVVPAQVLGVVAGLVPFVQNDEVTRITMATQQMSQAVPL
                     VKPQRPIIGTGIEAEVARNTDALIIAEDSGVVDYADAEKVIVKYSEKNRKTEYKTKKF
                     IQTNSGTSYNQVVKVLTGQKIKKGDILIEGPAVDEGEIAIGTNMKVAYMIYEGLEYED
                     GIIISDRIVKEDVLTSIHIQDYVVSVQETKLGPEEITRDIPNVSEDTLRNLDEEGIVT
                     IGSKVKSGDVLVGKVAPKGEVDLTSEERLLRAIFGEKAKDVRDTSLRMPHGEHGVVIG
                     VKTVTKEDNESLPAGTIKKITVYVAQQKKIEIGDKLAGRHGNKGVISAIVPAIDMPLL
                     PDGSPVDIIFSSEAVLKRMNVGQILETSLGAVGKKLGKYYEIPSLQRIPEERLLEEFK
                     KAGYPASGKMKLIDGRTGEYYHNDIVVGDAYILKLVHMSEEKMHARSTGPYSLINQQP
                     LGGKAQFGGQRFGEMEVWALEAYGAAHTLQEMLTIKSDDMMGRVQAFSALIQGNEIPG
                     STVPETFKLLVRKLNGLGLGLEAYSAEGTSEEETDTPVAAPEEEKVDGKGSEILDQEV
                     SVVDEEGLESEGLEEVKEESEDL"
     gene            9381..13049
                     /gene="rpoC"
                     /locus_tag="P5014_00325"
     CDS             9381..13049
                     /gene="rpoC"
                     /locus_tag="P5014_00325"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HRY56593.1"
                     /translation="MRDINKLKDFDALRIYLASTENILEWSYGEVTKPETINYRTFKS
                     ERDGLFDERIFGPSKNYECYCGKFKGIRYKGVICDKCGVEVTHNRVRRERMGHIKLAS
                     PVAHVWFFKGIPSNMALLLDLTPRNVESVVYFASFIVTEIDEKSKTKAVSQIGKDLEM
                     TKASLQKEIEDQVKALNKEIDDLKKTNDEMKAKEEELKIRQQIQKITSVYEKKVEEQE
                     KKFKLIQKKIESVEINSVISENEYITLISYLDKFCKLEIGADALKVILDRMDLNVLSE
                     QLREQLTKAKGQRAQKIAKRLVVVERFRRSGVTPGRMIMEVIPVIPPDLRPMVQLDGG
                     RFATSDLNDLYRRLINRNNRLKRLLDLGAPEIIVRNEKRMLQESVDALFDASKRKKKA
                     RTNTRGKELRSLADMIKGKQGIFRLNLLGKRVDYSGRGVIINGPDLKLNECGIPKEMA
                     LELFKPMVLREILARSYAPNVKSAKFYLDTRVPEVWDILEEVVDGHPVFLNRAPTLWR
                     LGIQAFYPKLVEGNAIKLHLCVCKGYNADFDGDQMAVHVPLTDKAIDEAKNILISTNN
                     LLNPSSGSPISIPSNIMLFGVYYITSIDEKLPVLNKVFFGETELMYAMEVKKEVDLRQ
                     LTKVRVGNEIKETTIGRIIFNKCLPEGFDYINEPMDKKRVTNLLTKVLKEEPNEVAVK
                     LIDDIKNIGLKYGTICGQSVSLSDIDIPAERVKMIDEGKKLVMGVDGYFARGLITKSE
                     VRKQKEDLWNKIIADLDNVVWDNLKEDNPVKILVKSGSTKASRDQVKQLAGMKGLIND
                     PTGKIVEIPIFGNFKLGLSALEYFVGSRGARKGLVDKGLKTADAGYLTRRLVDVSQDV
                     IVREEDCGTTEGREMIVGKGTLLQTFGDRVVGRVLAKDVKVGNKIIVKAGTFLTDKDV
                     AEIEKAGAEKIIIRSPMHCETRRGICATCYGSDLMTGKMVRVGTAVGVAAAQSIGEPG
                     TQLTMKTFHSGGIVGKDITQGLPRVEELVEARAPKFLSVMSEISGTARIEKNGDERKI
                     VVIPSDPGDQVVEYNIDPIEEILIKDGDTVGRGQKLTSGNLNLSDLYRTVGVDETKRY
                     VIEEVQGVYASQGVTINDKHLEVIVKQMFNHVKIEDAGDTEFMVGELTTRARFDEETE
                     RVVASGGTPATGKLVMLGITKAALHTDSWMSAASFIHTSTVLTEAACSGKVDYLMGLK
                     ENVIIGRLIPTGERARVN"
     gene            13052..13474
                     /gene="rpsL"
                     /locus_tag="P5014_00330"
     CDS             13052..13474
                     /gene="rpsL"
                     /locus_tag="P5014_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016338469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HRY56594.1"
                     /translation="MPTINQLLRKSRKKKVKKASTPALKEVFNTKRKTISKRNSPLKR
                     GVVLLVRTQTPKKPNSALRKVARVRLSNKREVTAYIPGEGHNLQEHSVVVVKGGKVQD
                     LPGIKYEIVRGTADLAGVEKRKTSRSIYGTRKVKEKKA"
     gene            13471..13938
                     /gene="rpsG"
                     /locus_tag="P5014_00335"
     CDS             13471..13938
                     /gene="rpsG"
                     /locus_tag="P5014_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012463017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HRY56595.1"
                     /translation="MRGKRAKKRALMPDSIYKSKVVARTINTVMERGKKSVAKSIVYG
                     SIQKLNQDEKEGLRIFEQAVKNVMPQQEVRSKRVGGATYQVPMPLKHDRAEALAIRWI
                     VDAAKSKKGKPMVEKLYEILNDSYQNIGDAIKKRDDVHRMADANRAFAHFARF"
     gene            13941..>14686
                     /locus_tag="P5014_00340"
     CDS             13941..>14686
                     /locus_tag="P5014_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003559494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRY56596.1"
                     /translation="MSDTADKKYPLEKIRNIGIIAHIDAGKTTTTERILYYTGKSHKI
                     GDIDEGNTQMDWMAQEKERGITIQSAATTCFWKDHRINIIDTPGHVDFTAEVERSLRV
                     LDGGVIVLDGSQGVEPQSETVFRQAQKYDVPLLFFVNKLDKVGGDFFMSVNSVAEKLT
                     KKGVAVQIPIGIENGFEAVIDLIEMKAYKFEGSGGEKVEEFDIPQDYLEDAKKYRSIL
                     VERVAESDDSLIEKYLNGGELTIEEIKKAI"
BASE COUNT         5108 a         2107 c         3139 g         4332 t
ORIGIN      
        1 ttgtcctttg ataccacgct tcacctcgga acattattcg ctcttattgt tgttttttgg
       61 aaagatattt tatcaataat taagtcactt tttttggata ttaagaagca taaaaaatct
      121 ttcaatcact actcctcgaa atcttggttg gctttcaaaa tttttatcgc atcgatacct
      181 gtagggttag cagggctctt cttcggagat tttttagaaa ataaatttag aggaatttgg
      241 ttcgtaatta tatttttgtt agtgggaact cttataatgt acattgctga aaaaagattt
      301 aaaaaaagat tgattgttaa ggatgaaata tccgtcggta aaagttttag agtaggaatt
      361 tttcaagctt tagctctttt acctggtata tcgaggtcgg gttctaccat ttccggaggt
      421 atgttctttg gactaagtcg gaaagaggct gccaggtttt cttttttgct atcaattccc
      481 gcggtcttgg ccgcaggagt ttctcaaatt atttcgtctc tggattattt aaatatggcg
      541 gaaattgccc ccatgatggt tggttttttt tcaagttttt tagtcggtat aatagcaatc
      601 aattttatga tgaagtttgt taaaaacaac aagctttacc cttttatatt ttacaggtta
      661 ggtttagcag tatttttaat aatcttattc tacatctaag caaatctgct aaaatcatct
      721 tgttatgttc ggttcattat ttgacacaaa tgaaaaacaa ttgaataaaa tatggcctgt
      781 tgttgaagaa gttaattctc ttgaggaagg tgttaagaag ttaagtaagg aagaaattca
      841 gggaaaaaca aaaaaatgga aagaagaatt acgtaagttt gatgagaaag aaattgataa
      901 ctacttaaat aaaatacttc ccgaagctta cgctttagtc agagagtccg ctaccagaac
      961 tgtaaatatg agacactatg atgttcaaat cttagctggg atagttttac atcaaggaaa
     1021 aatcgctgag caaaaaacag gtgagggtaa aactcttacc gccacacttc ctatatatct
     1081 aaattccctt ggcgggaaag gtgttcattt ggttacacct aacgattatt tatcaagaca
     1141 cggtgccggt tggatgggag ctctttatga ttatttagga gtttctgtag gggtaataat
     1201 gcaagaaagg gcttttaaat atgatagttc ttttcaagga accgaatttg aagattctta
     1261 ttctgtccat ttaagagaag taaccagaca agaagcttat caatgcgaca tcgtgtacgg
     1321 aactaatcat gagtttggat ttgattattt aagagacaac atggttaggt ctctttctga
     1381 tatggttcaa accaatccga gaggagattg gggtgttcac aattttgcaa ttgttgatga
     1441 agttgactct attttaatag atatggcaag aactcctttg ataatttcca ctgctgatgc
     1501 aaaaccgagt gaaagatatc accaagcaaa tggaattgtt aaatctctta ttaaagatac
     1561 tgactacgaa gttgaggaga aatataaaaa tgtaacttta accgatttgg gtatcagaag
     1621 agtagaacga cttttaggaa tagagaattt atatgagcag gattttgaaa tggttcattt
     1681 gatggagcag gctctttctg caaataccct ttatgaaaaa gataaagact atgttgtcaa
     1741 aaacggagct gtggttattg tagatcaatt tacaggtaga ctacttccga gcaacaggtt
     1801 ttctcacgga cttcatcaag ctatcgaagc taaggagggc gtgaccattc agcaagaatc
     1861 aaaaactctt gcagaaattt cgtatcaaaa ttattttaga atgtacggaa agcttgcagg
     1921 tatgaccggt acggcagcta ccgaatctga agagtttttt aagatttata aattagaggt
     1981 cgttattatc cccactaata aaaagtgtat tagaaaagat aacaacgatg ttgtttacaa
     2041 aaccgaagca ggaaagttta aagctgtagc agatgaaatt gaaaaaagat ataaaaaagg
     2101 acagcctgtt ttagttggaa ctacgtcagt tgataaaagt gaactccttc atcacttttt
     2161 gcagagaaga aatattcctc acgaaatttt aaacgcaaaa aatcatgaaa gagaggcttt
     2221 gataatcgct caagccggac gagaggggtc agtaactatc tctactaata tggcaggccg
     2281 tggtgtggat attattttag gaggggaccc ggttgatttg gatatgcaaa aaaaggtggt
     2341 cgaggccggt ggtctttgtg ttataggtac agaaagacat gaatccagaa gaattgataa
     2401 tcagttaaga ggaagatccg gacgacaggg cgatcccggt gagtcgaagt tctttgtttc
     2461 cttacaggac gacctgatga gaattttcgg tggcgaatct gtttcaagaa ttatggatag
     2521 gtttggtttg gacgagaata tccctttgga agcaggcatt gtttccaagt ctatagaaaa
     2581 cgcacaaaag agagtggaag gttttaactt tgacagaaga aaacagttag ttgagatgga
     2641 cgatattatg aatactcaca gggaggttat ttataaatta aggagaagaa tactagagct
     2701 cgggggcggg gttgaagatt acaaagattg gttggttgaa aagttttcag aattttcttc
     2761 tttgagtagg gagttttggg atgagcgtga aaaggaattt ggaaaagaaa catggtttca
     2821 aattgttagt ggatttggat gtcctgttat agattttatg tggatggacc atttggttga
     2881 tatggatcag ttaagggaag gtattggatt gagaggttac gcgcaaagag atcctatggt
     2941 tgaatataaa agagaaggtc atgaaagatt tgagatctta gtttctaagg tttattcgat
     3001 ggtaggagaa agattatctt caatttcaaa agatgctgtt cctgaaaaaa gttctcattc
     3061 ggatgtttct agaaaactta catatcataa aagtgagttg gagacaggtg tttctgaaga
     3121 gggtatggaa atgagcgaga gaaaagtagt cgatgattcg gggcgagaaa tgaaggtaga
     3181 aaaaattgaa tctggtagag agaaggtggg gaggaatgat ccatgtcctt gtggaagtgg
     3241 gaagaagttc aaaaattgtc acggaaaaaa tatctaacct ccaagttttt tagtgcgcca
     3301 caatttttcc gtcacttcgt gatgtagaac tgccatggta gaaacgtcta gaattattta
     3361 ctaacttttg cactgcagtt cttgcgccgc tttgcgtcca ataattttca cgagagtagc
     3421 atttttcctc tattattttg taacgctcta aaattcttgc atcactatgt gatgaaaaaa
     3481 attgtcacgg aaaaaacatt tgattctagg ttttgtactg cgccacaatt tttgtgtcgc
     3541 tttgggttca aaaaattatt gagctagcat ttggtttgcg gtttgaagac ccgcgttaca
     3601 gtcagcgctt gtaggttcat attgtaaaca agcttcaaaa tactttttag cattttcaaa
     3661 atctttttcc tccatgtaga taattcctag tttataagct gatgggtata accttggatt
     3721 catttgtaat gattgttcca gatatcttat ggcttgaggt atgttacctt gtaccatata
     3781 tatgtgaccg atattatgta ccgcgtctgc gtaatcaggt tttagcgcat atgatcgttt
     3841 aaagttttcc aaagctccct caagatctcc ttcatttgca agaacatcgc ccatgttgtt
     3901 ataaactctg taactgtagg gagcagtttt cttggaagca atccacaagt ttttactatt
     3961 tctcaaatca ttatttctaa gaaccgttct taaagagtac aaagaaatta ttatgattga
     4021 tgcgtataga acaaaattat ttttctctac accttttttt acctttcctt gttttttatt
     4081 tttctcgtta attttatttt cgaagtatat taatccgatg ccaattattg ctgatgaaaa
     4141 tattgaaggt atataaaggt atctctcggc agctatccaa gcaactataa ccggactaaa
     4201 agaagggagc actccgaaaa ttatcatcaa aagtagtcct gaaattcttc tgtctttttt
     4261 cataaagtaa aatatggcaa agataacggc aaaggttaaa aacaccataa atgtatattc
     4321 tgcgggtgtt atgtatttac cttcatggta aatacttaat gtttttgggt aaaacaatgt
     4381 tgtgtattcc atatatattg tataggggat tctattgatt agaggtgtgg ctgttgcagt
     4441 atcaacgtaa tacagagttt ctaaaccctg aaccctttct ttaatatatc ctggtagtag
     4501 agcaatcgcg aaagctaaag aggttaataa gtatggtaga tactgtttaa tatttgaaaa
     4561 tttaatcttt ttttcaatta taaactgatc cattaaaact aataagaatg gtgtcactaa
     4621 aatccaaccc ccacccctat aaaaaataag tgagaaaaca tagataagag ttgatattag
     4681 caaatagatt ttattatcgg tttttttaaa aagtgcaaaa aatattaatg ttgggataat
     4741 tatcgaggct cttcccgcat accaaacacc cgcatgccaa ctaatggctt cggtgtttgc
     4801 aggatgagct ataaaaagta aagttccaat tattgatatt ttcttgccaa aaagtatgtt
     4861 taaaagaata aaaactaaaa taccatttaa catatgaaac attatcgaaa cgatatgaaa
     4921 aacagacgaa ttaataccaa atgtttggac gacaacagac attacaatag taaaagcatc
     4981 aaaagatttt agtgctagtc caaaatcatg aaactcaggt gatgtgaaag cctttgagta
     5041 atcatctaaa ttaacaaaat ctccggtgat aatgttggca tagattaata atgtaaatat
     5101 tgttattacg acaatgaata atttattttc tttaataaac tttttgaaag tcttaaaatt
     5161 ataaaaagtt aagtctgaat tatttactac atcgactgat tttatttctt tgtctttttt
     5221 catcggaatt cattttacta tacaatttaa aattcttcac ttatcattta atgatatttg
     5281 gattattatt tgttggatta tgaaaattaa aagttttatt ggcgagataa ttgagacagt
     5341 tttgctttca gccgtggtaa tactaatgat ttaccatttt atcgcatctg ttgaagttgt
     5401 ttggggacca agtatggagc cgaattttca ctcaggagaa agaatattag tggatagagt
     5461 aaccaccaaa ttttcggaat taaaaagagg agatgtggta gttttttacc cgcctaatga
     5521 tgattcaaaa cattatataa aaagagttgt gggaattccc ggagatgttt ttaaaataat
     5581 aaactgtaat gtggtaattt cccgagatgg gaaacattat gttttatccg aaaattattt
     5641 gtcagaaggt gtgtgtactg aggctggttc ttttataaaa gagggcaggt caataaaact
     5701 aacagacaat caatatgtcc ttttaggtga taacaggccc caatctcttg attcgagaaa
     5761 catcggtgtt atagataaaa gcaggttggt aggtagagtt gtatttagat tttggccaat
     5821 atcaaaaatt gggtttataa aataggtatt gacacatttt tgttattatg gtaactttcg
     5881 cactgtccca aacacgtccg caaaaaataa taggtacaaa gatacccagt agggatgggt
     5941 ttctttttat ctttatttca gtttggtact ttcaaaaaat ttatatttca ctagttaatt
     6001 ctttatttga aaaggcttat aattaaaatt taatgcgaga aactttttta aaaagaaaaa
     6061 tagctcttcc tttaccaaat ttagtgggag tacaacttga gtcattcgat tggcttaaaa
     6121 gagttggttt gaaagaagta ttggatgagc tcggtactat agaagataac tccggaagag
     6181 gatgggtttt aaccctatct gatccaagag ttgataaaga aaatattacg atagaggaag
     6241 ctaaaagaac cggaagaact tacgatgctc cttggtatgt acaagcttcc attgaagatc
     6301 caatttccaa aaagaaaaaa gtaaatgaga tttatatggg agatattccc ctgatgacag
     6361 atcaaggtac ctttgttatt aacggtgttg aaagaattat tattagtcaa ttaataagat
     6421 ctgagggtgt tttattcggc ggggagaact ctcccgttac gggacaattt cttgctgcag
     6481 ctaaagtact tcctaaaagc ggtgtctggc tagaattcga aacttccagg accggtgtta
     6541 ttaccttaag aatagataaa aaaagaaaaa ttaccgccac aacattattg agagttttcg
     6601 gtttggaatc agatgacgat ataaggcaag cttttgtagg tgtggaaaca aatcccgaaa
     6661 ttaattacac tgaagctact ttagccaaag atcctgcaga agggtttgat gatgcttgtt
     6721 tagaaattta caagaaaatg agacccggtg agcctttagt tatcgataat gcaaaggcat
     6781 tggttcatgc tatgttcttt aataaacgaa ggtacagttt ggggtccata ggtaggttta
     6841 aactaaacca aaccttgggt ttaaactttc ctaatgatcc taagcacaaa cttattcaac
     6901 tcgaagacct tataaaaata ctctcaagaa taattgagct taataatggt attggtgatc
     6961 ccagcgacgt tgattttttg ggcaacagaa gagtaaaaag tatgggagag cttttacaat
     7021 ggcagatgag agttggtttc ttgaggatgg aaaagaatat taaagaaaga atgagtcttg
     7081 ccccgaggga caggttacct gaaccttcca cattaatagc tccgagagca atcgctgctg
     7141 cagtacattc attcttcgct accggacagc tttctcagtt acacgatcaa caaaatcctt
     7201 taactgcact tgatcattta agaagacttt ctgtcttggg accgggtggt ttatcaaaag
     7261 atagagcaag tttttcagtt agggatgttc acaattcata tttcggaaga atttgcccag
     7321 caagaactcc tgaaggacct aatgttggac tcataaatta catgtctatg tatgcaagag
     7381 ttaatcaata cggattttta gaaacacctt atataaagct cgaaaaagat tcaaaaggaa
     7441 atgtaaaaat tactgatgag gttgtttatt tagcagctta cgatgaagag catacaaata
     7501 ttacagacca atcagttgaa atagatgaaa aaggttatat tttagacgaa cacgtaccaa
     7561 taagaaaggg aggagagttt ttcttaggag acagagcaac ggctgaatac attgaagttg
     7621 ttccggcaca agttttaggt gtagtcgcag gactggttcc ttttgtacag aacgacgagg
     7681 ttacacgtat aaccatggct acacagcaaa tgtcccaggc agttcctttg gtaaaacctc
     7741 aaagacctat aattggtacg ggtattgaag ccgaggttgc cagaaatacc gacgccttaa
     7801 ttattgctga ggatagcggt gtggtcgatt atgccgatgc agaaaaggta atagttaaat
     7861 actccgagaa aaacagaaaa actgaataca aaacaaagaa atttattcaa acaaatagtg
     7921 gaacaagcta taaccaagtt gttaaagtat tgacaggcca aaagataaaa aaaggagaca
     7981 tccttattga aggtcctgca gtagatgaag gtgaaattgc catcggaacc aatatgaagg
     8041 tcgcctatat gatatatgaa ggtttagagt atgaagatgg aattatcatt tcagatcgta
     8101 tcgtaaaaga agatgtctta acttcaattc atatacagga ttatgtagtt tcagttcaag
     8161 aaacaaagtt aggtcctgaa gaaatcacca gagatattcc taatgtttca gaagatactc
     8221 taagaaattt agatgaagag ggtattgtta ctattggatc caaagttaaa tctggagatg
     8281 ttctggttgg taaagtagca cctaaaggag aagttgattt aacttcagaa gaaagactat
     8341 taagagcaat ttttggtgaa aaagctaaag atgttagaga tacttcttta agaatgcctc
     8401 atggggagca cggagttgta ataggtgtta agactgtaac aaaagaagat aatgaatcgc
     8461 ttcccgcagg aacgattaaa aaaattactg tatatgtagc acaacaaaag aaaattgaaa
     8521 taggtgataa acttgcaggc agacacggta ataaaggagt tatttcagct attgtacctg
     8581 caatagacat gcctttgctt cctgatggtt ctccggtaga tattattttc tcatcagaag
     8641 cagttttaaa aagaatgaat gtcggtcaga ttttggaaac ttctcttggg gcggtaggca
     8701 aaaagctggg aaaatattac gaaattccat ccttacaaag aattccggaa gaaagattac
     8761 ttgaagaatt taagaaagcc ggttatccgg caagcggaaa aatgaaactt attgacggta
     8821 ggacaggtga gtattatcac aatgacattg ttgtcggaga tgcttatatt cttaaactag
     8881 tccacatgtc tgaagaaaag atgcacgcaa ggtcaacagg cccttattca ttgattaacc
     8941 aacaaccttt gggtggtaaa gcacagttcg gaggtcagag attcggagaa atggaagtct
     9001 gggcattgga agcatacgga gctgcacata ctttacagga aatgttgaca atcaaatccg
     9061 acgatatgat gggtagagtg caggcattta gtgcattaat acaaggaaac gaaattcccg
     9121 gatctacagt ccctgagacc tttaaactat tagttagaaa attgaatggt ttgggattag
     9181 gtttagaggc ttattcagca gaaggtactt ctgaagaaga aactgataca ccggttgcag
     9241 cacctgaaga ggaaaaagta gatgggaaag gttctgaaat tcttgatcaa gaggttagtg
     9301 ttgtagatga agaaggtctt gaaagtgaag gtttagaaga agtaaaagag gaatcggaag
     9361 atttataaaa ggataaaaat atgagagata ttaataaatt aaaagatttt gatgcactta
     9421 gaatttacct tgcatcaact gaaaatattt tagaatggtc ctatggggag gttaccaaac
     9481 ctgagactat taactataga acttttaagt ccgaaagaga cggtcttttt gacgaaagaa
     9541 tttttggacc ttcaaaaaac tacgaatgct attgcggaaa atttaaagga ataagataca
     9601 aaggtgtaat ctgtgataaa tgcggagtcg aagttaccca taacagagtt agaagagaaa
     9661 gaatgggtca cattaagtta gcttctccgg tagcgcatgt ctggtttttt aagggtattc
     9721 cttcgaatat ggcgcttttg ctcgatctaa ctcctagaaa tgttgaatct gtagtgtatt
     9781 ttgcttcatt tatcgtaacg gaaatcgatg agaaaagtaa aacaaaagca gtttctcaga
     9841 ttggaaaaga tttagaaatg accaaagcat ctcttcaaaa ggagattgag gatcaagtca
     9901 aagcactgaa caaagagatc gacgatttga aaaagacgaa cgatgagatg aaagctaagg
     9961 aagaagaact aaaaatcaga caacagattc aaaaaataac ctcggtttac gagaagaaag
    10021 tagaggagca ggagaagaaa tttaaattaa ttcaaaaaaa gattgaaagt gtagaaataa
    10081 actcagtaat ttccgaaaac gagtacatca cactcatctc ctatttagat aaattttgta
    10141 aacttgaaat tggtgcagat gctcttaaag taattttaga cagaatggat cttaatgttt
    10201 tgtctgaaca actaagagaa cagcttacta aagcaaaggg tcaaagagct caaaaaattg
    10261 ctaaaagatt agttgtagtt gaaagattta gaagatctgg tgttactccg ggaagaatga
    10321 ttatggaagt aattcctgtc ataccgccgg acttaagacc tatggtccag cttgatggtg
    10381 gaagatttgc tacatcagac ctgaacgatt tgtatagaag acttataaac agaaataaca
    10441 gattaaagag acttttagat ttaggtgctc ctgagattat cgtaagaaat gaaaaaagaa
    10501 tgttgcagga atctgtggat gctctatttg atgcttcgaa aaggaagaaa aaagccagaa
    10561 caaacacccg aggtaaggag ttaagatcac ttgccgatat gataaagggt aaacaaggta
    10621 ttttcagatt gaatttactt ggaaaaagag tcgactattc aggtagaggg gttattatca
    10681 atggacctga tttgaaatta aatgaatgcg gaattccaaa agagatggcc ttagagttgt
    10741 ttaaaccaat ggttttgaga gaaatattag caagaagtta cgcacctaat gttaagagtg
    10801 caaagttcta cttagacacc cgagtacccg aagtttggga tattttagaa gaagttgtag
    10861 atggtcaccc ggtattttta aacagagctc ctacactctg gagattaggt attcaggcat
    10921 tttatccaaa attagtagaa ggtaacgcaa taaaattaca cctttgtgtt tgtaagggtt
    10981 acaatgcaga ctttgacgga gaccaaatgg ctgttcacgt tcctttgact gataaagcta
    11041 tcgacgaagc aaagaacatc ttaatttcaa ctaataatct tttaaatcct tcaagtggat
    11101 caccaatatc aattccttct aacatcatgt tatttggtgt ttactacata acatccattg
    11161 atgagaagct tccagtgtta aataaggtgt ttttcggaga gaccgagtta atgtatgcaa
    11221 tggaagttaa aaaagaagtt gatttaagac agttaacaaa agtaagagtc ggtaatgaga
    11281 ttaaagaaac aactattgga agaattattt ttaacaaatg tttacctgag ggttttgact
    11341 acataaacga accgatggac aagaagagag tgacaaattt gctgactaaa gttttgaagg
    11401 aagagcctaa tgaagtcgca gttaaactaa tagatgacat taaaaatatc ggattaaaat
    11461 acggtactat ctgcggtcag tcagtttcat tgtctgatat tgatatcccc gccgaaagag
    11521 tgaagatgat tgacgaaggt aagaaattag ttatgggtgt agacggttat tttgcaagag
    11581 gtcttataac aaaatctgaa gtaagaaaac aaaaggaaga tctatggaat aaaataattg
    11641 ctgatcttga taatgttgtt tgggataact tgaaagaaga caaccctgtt aagattttag
    11701 ttaaatcggg atctactaaa gcttcgagag accaggttaa gcaattggcc ggtatgaaag
    11761 gattaattaa cgatcctaca ggtaaaattg tcgaaattcc aatatttgga aactttaaac
    11821 ttggtttgtc agctttagag tattttgtag gttcaagagg tgctagaaaa ggattagttg
    11881 ataagggttt aaaaacagct gacgcaggtt atttaacaag aagattagtg gatgttagcc
    11941 aggacgtcat agtaagagaa gaagactgtg gaaccactga aggtagagaa atgatcgttg
    12001 gaaagggaac acttcttcaa acattcggag atagagttgt aggaagagtg ttagctaaag
    12061 atgttaaggt aggaaataaa ataatagtta aagccggtac tttcttaaca gataaagatg
    12121 tagcggaaat tgagaaagca ggagctgaga aaataataat cagatcacct atgcactgtg
    12181 aaaccagaag aggaatttgc gccacttgtt acggttctga tttgatgaca ggaaaaatgg
    12241 ttagagtagg aaccgctgtt ggagttgcag ctgctcaatc aatcggagag cctggaacac
    12301 agcttaccat gaagacattc cactccggcg gtattgtcgg aaaagatatt actcaaggtt
    12361 taccaagagt tgaggagtta gtcgaggcaa gagctcctaa atttttatca gttatgagtg
    12421 agatttcggg tacagcaaga attgaaaaaa atggtgacga aagaaaaata gtggtaatac
    12481 cttcggatcc tggagatcag gttgttgaat ataacattga ccctattgaa gaaattttaa
    12541 taaaagatgg agatacagtt ggaaggggtc aaaaattaac tagtggtaac ttgaacttaa
    12601 gtgaccttta tagaacagtc ggagttgatg agacaaaaag atatgtcatc gaagaagttc
    12661 aaggagttta cgcttctcaa ggagttacta tcaatgacaa acacctagaa gttattgtta
    12721 aacaaatgtt taaccatgta aaaattgaag acgcgggtga cacagaattt atggttggtg
    12781 agcttaccac aagagcaaga tttgatgagg agacagaaag agttgttgcc agcggaggta
    12841 ccccggctac aggtaaatta gtgatgttag gtattactaa agctgcttta catacagata
    12901 gctggatgtc agcagcgtct tttatacaca caagcactgt attgaccgaa gccgcttgct
    12961 ctggtaaggt tgactacttg atgggcttga aagaaaatgt catcattgga aggttaattc
    13021 ctaccggaga gagagcaagg gtaaattaaa aatgccaact ataaaccaat tattaagaaa
    13081 aagtagaaag aaaaaagtaa agaaggcaag tacgccggct ttgaaagaag tttttaatac
    13141 taaaagaaaa actatttcaa agagaaacag tcctttaaaa agaggagttg ttttattggt
    13201 tagaacacag acacctaaga aaccaaattc tgctttgaga aaagtggcaa gagtcaggct
    13261 ttcaaataaa agagaagtaa cggcctatat tccgggagaa ggtcacaatc ttcaggaaca
    13321 ctctgttgta gttgttaagg gtgggaaggt tcaggatctt cccggtataa agtatgagat
    13381 tgttagaggg actgctgacc ttgcaggagt tgaaaaaaga aaaacatcaa gatcaattta
    13441 tggaacaagg aaagttaagg agaaaaaagc atgagaggta aaagagccaa aaaaagggcg
    13501 ttaatgcccg attcaattta taaatctaaa gttgttgcca gaacaataaa tactgttatg
    13561 gaaagaggta aaaagagtgt agcaaaaagt atcgtttacg ggtctattca aaaattaaat
    13621 caggatgaaa aagaaggctt aagaatattt gaacaggccg ttaaaaatgt tatgccacaa
    13681 caggaagtta gatcaaagag agttggtgga gcaacctatc aggttcctat gccattaaag
    13741 catgatagag cagaagcttt ggcaataaga tggatagtcg atgccgcaaa atcaaaaaaa
    13801 ggaaaaccaa tggtagaaaa attatatgaa attttgaacg attcctatca aaacattggt
    13861 gatgctatta aaaagaggga tgatgttcat agaatggcgg atgcaaacag agccttcgca
    13921 cattttgcaa ggttttaaat atgtctgaca ctgctgataa aaaatatcct ttagagaaaa
    13981 ttagaaatat tggaattatt gcccatattg atgccggaaa aaccaccact accgaaagaa
    14041 tcctttatta tactggaaaa tctcataaga ttggtgatat tgatgagggt aatactcaaa
    14101 tggactggat ggcccaggaa aaagaaagag gaattactat tcaatcagct gcaacaactt
    14161 gtttctggaa agatcataga ataaatatca ttgacacccc aggccacgtt gattttaccg
    14221 cagaagttga aagatcactt agagttctgg atggtggagt tattgtttta gatggttctc
    14281 agggagttga gccacaatct gaaactgttt ttagacaagc tcaaaaatat gatgtcccct
    14341 tgttattttt tgtaaataaa ttagataaag ttggtggtga cttttttatg tccgtaaact
    14401 ccgttgcaga aaaattaacc aaaaaaggag ttgctgttca aattccaatc ggaatagaaa
    14461 acggttttga agctgttata gatttaattg agatgaaagc ctacaaattc gaaggatccg
    14521 gaggtgagaa ggtagaagag tttgatattc cccaagatta cttagaagat gctaaaaaat
    14581 acagatctat tttagttgaa agagttgctg aatcagatga ttccttaatt gaaaaatatt
    14641 tgaacggcgg ggagctcact attgaagaaa ttaaaaaagc tattag
//
LOCUS       DAOTFA010000004        13808 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate AMR_MDS_3115
            NODE_3069_length_13808_cov_6.117429, whole genome shotgun sequence.
ACCESSION   DAOTFA010000004 DAOTFA010000000
VERSION     DAOTFA010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868139
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (wastewater metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 13808)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13808)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:36:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 536
            CDSs (total)                      :: 521
            Genes (coding)                    :: 520
            CDSs (with protein)               :: 520
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13808
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3069_length_13808_cov_6.117429"
                     /isolate="AMR_MDS_3115"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..993)
                     /locus_tag="P5014_00345"
     CDS             complement(<1..993)
                     /locus_tag="P5014_00345"
                     /inference="COORDINATES: protein motif:HMM:NF024724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRY56597.1"
                     /translation="MLTENFETQYKKNLKTSDRKILAALKIAQEKHKNLYRKSGEPYI
                     EHCIAVYNILGEWGVNDTNLLIAALLHDTLEDTNLKIEEISKYFGEEVAFLVDSVTKI
                     RLGVKEGQDFKTLKKIVGTSYVDPKVSVLKLADRYHNLSTLQYLPNSNRKKKAKESLE
                     IYAKLAESLGMWVVKTKIEDLSFQYLNFDNFSKVKTSIDCDKRLSSTEIEKNLKTIRN
                     ALEKTKIKANVETKIGGYYSTYQKLKSNAVKGLASSEDYKKINDVISYRVAVKSIEQC
                     YEAIYAIHNVYGGKVDFNRFDEFIGANKRINGYEALQTTIETDFGSIEIAIAKKD"
     gene            complement(1177..1262)
                     /locus_tag="P5014_00350"
     tRNA            complement(1177..1262)
                     /locus_tag="P5014_00350"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1226..1228),aa:Tyr,seq:gta)
     gene            complement(1406..1488)
                     /locus_tag="P5014_00355"
     tRNA            complement(1406..1488)
                     /locus_tag="P5014_00355"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1452..1454),aa:Tyr,seq:gta)
     gene            complement(1553..2767)
                     /locus_tag="P5014_00360"
     CDS             complement(1553..2767)
                     /locus_tag="P5014_00360"
                     /inference="COORDINATES: protein motif:HMM:NF016032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - cellular modified amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-cysteine ligase family protein"
                     /protein_id="HRY56598.1"
                     /translation="MLSAFNKMSKEQISKVPKGFPTPTITMVGAEQELFIYREVEGKS
                     ELITDEEKNEMLAKLGDDFGPELGASCIEVHPAPVDLTKVGFSGWLAQLRDLEKQLWV
                     VVEKHGCKVGRYGTIPWADTQNIQRTKTPKYTKVPTFHNLNKTRKIEETINGVNVLDA
                     AIVGILNAFQFSVQCNGLEDAVDKLNRLYMISPLSVALSGNARFLGGVDTGWEDVRFE
                     AWKRSHDTRSEQDIAEGKSLRVGLPNNYFNSIEDYMKEVSSYPFILEDEAHALQIGIG
                     LFWRDARIKFIKDKAIVEFRPVSTQKTVEEEIEIAAFTIGRLIYSQSLREELIQMPHV
                     SLNKKEAEIHGLRGSYIYNVGLAIKTSEIMLAEIERAQAGLKISGIPDSSSFSFLKYK
                     DKIAKIKAETGR"
     gene            complement(2838..3770)
                     /locus_tag="P5014_00365"
     CDS             complement(2838..3770)
                     /locus_tag="P5014_00365"
                     /inference="COORDINATES: protein motif:HMM:NF016817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HRY56599.1"
                     /translation="MKKTITKIKIGQAGAVSIEIPVLKFTGSKRGKKALIVCGIHGDE
                     YTGLLVINNVLEKIKDIQGELWIIPSANALAQALNIRINPLDLYDLNRKFPGDKTKEP
                     TDRIAAEIFEIAKDMDAVLDFHTFEDPTVVTNIFMNCGSNETKKKTLEFIKLFDPDIV
                     WELNIKSRDELKFVTALGPKLSFEGIPNIAVELPQLHNVSDEQIERAVDGTIKILNNL
                     KITNVSHAKESRELLFVENKEFNSNVSGIFVTHKKLMDKIKKGEVVGGVIDITSFKKT
                     QIKSHESGVLMVLKDRQFVTTGGRLFTVGKLKRK"
     misc_binding    complement(3834..4074)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(4202..5209)
                     /locus_tag="P5014_00370"
     CDS             complement(4202..5209)
                     /locus_tag="P5014_00370"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRY56600.1"
                     /translation="MNSKNTFGKEPNYGRLLGFIVLLIVTWGIVPAFGKLANMPGDVT
                     TFYVNVTALIALALIITIMRYKDQNGVARTYWTKLKNYSFKDYGLMVAIGVVWPLIYS
                     LFYFQAIYEGEPVGMTILNYTWPLMVIALSILKYGKKAYYVTKSGAKFIIPVILTVAA
                     VVFYQMDAIVSFTTRILVMGLIAALTQALFNFIDHGYDNWIALFVIEATTVVFIVPIM
                     IFTTHYTMVPSLNSLLLMSIIGVAGNAIGFWVFMEGRRECGRLKERGVMTSEPIWLAA
                     QGVVPISVLVTMIITNLVSKNEQPFAITTNQIISLVVLAAGLVLFFIFKNIEDHKIHT
                     Q"
     gene            complement(5249..6487)
                     /gene="tyrS"
                     /locus_tag="P5014_00375"
     CDS             complement(5249..6487)
                     /gene="tyrS"
                     /locus_tag="P5014_00375"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HRY56601.1"
                     /translation="MRNIYELLKERGYIATVEEQGIKKFQVTDEEAVSNLLTQGTTVY
                     EGFDPSADSLHLGHFMSIMALHHMQEAGNRIIFILGGGTGRVGDPSGKNKARSLLTKE
                     MVIKNATAIKNQVQSMGLLDLDNPKKALMIDNSDWLAKALFLDDYMMNVARYFSVNEL
                     VKMETFAKRLSEGAPLSLLEFLYATMQSWDYLELYEKYDCRLQVGGSDQWGNILQGVS
                     LIKDHKGNKANAHALTFPLLTTANGEKMGKTVSGPLWLDPKKTSPFDFYQYIEKIPDD
                     MVRKLFGLYTFIPMEEIDKIMAGNPRDAQKKLAFEVTKVVHGEEEAKNASKQSTMVFT
                     GSSENLGSIPEFKLSNSMRLDEILVTSGSLVSNSEVARRVKGGAVKIDDQKATDPKQQ
                     INKPVLIKYGKTNFLKVVSS"
     gene            complement(6765..7892)
                     /locus_tag="P5014_00380"
     CDS             complement(6765..7892)
                     /locus_tag="P5014_00380"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRY56602.1"
                     /translation="MDKEVTELLKKLIGIPSYLGDGVDESIFAEFIYKFLIENTDLKV
                     EKQTVEGKRFNIIAYNNKKPSIIFFSHMDTVPPKISTPEPFKAKKSGNKIYGLGSVDM
                     KAGLSVCLFLAKKFKSSKNIAYIFTVDEEYAFKGALKLLEKYQFKPDIIINPEPTSLE
                     ISNGCRGVTEFSFNVHGKSCHAGTKYLGINAIEKAITMFEMLQKDISKYDNEGGNNSL
                     NLAHLNGGILKNDGSISYSGNVVPDYAFCVSEIRLSDKRISRKWIEDKIETNAKELGI
                     KVSEIKFKFLMKPAYTPKSKLEGFEEIVKQSGIKPIYKDIDSTGYFEVQLLQESWGSD
                     ILVFGPGPSDQAHKENEYVDIDSVIKTNEVFEAYIKTLSIE"
     gene            7985..8683
                     /locus_tag="P5014_00385"
     CDS             7985..8683
                     /locus_tag="P5014_00385"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRY56603.1"
                     /translation="MKKCELLVIIQQTQVKPVLRFIDKIEYMMINYPLKEGKIIQPFG
                     RDASDDPIYKEFYKIFDYKHCGIDFDTEIGTNVYAAFSGIVVRAENHEGMGNVIGIRN
                     GNLVGLYAHLSEIGVILGQLVSTGDLIGKSGATGKACPKPHLHFELRDLTKSSLKEMV
                     FEPIFEKEIDRYKDTFTYTVNNKNTQKTLRSLSKLYFGTQNYWELIKIANGFDFDESI
                     VLEEASEIIIPNYN"
     gene            complement(8942..9024)
                     /locus_tag="P5014_00390"
     tRNA            complement(8942..9024)
                     /locus_tag="P5014_00390"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8988..8990),aa:Leu,seq:tag)
     gene            complement(9630..10196)
                     /locus_tag="P5014_00395"
     CDS             complement(9630..10196)
                     /locus_tag="P5014_00395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56604.1"
                     /translation="MSKKILKKQLKILPFVAIFVWMAILVTVENNRPLDASMSFIGKE
                     IDTGREEKIESLEKFFEAQKSPLKEDADTFVRVAEKYGLDYRLLPAISCMESSCGKRM
                     LPNSYNPFGWGIYGKNAIYFKSFEEAIETVGKGISEKYVAKGLDTPEKIAPVYTPPNS
                     INWRNGVNFFISKIENPKLIDRSILFAS"
     gene            complement(10278..11117)
                     /locus_tag="P5014_00400"
     CDS             complement(10278..11117)
                     /locus_tag="P5014_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56605.1"
                     /translation="MNRVNSNNCFYKNGKKILKKKISNFYSKEEINSLSSALINENIF
                     SIAGGFEDTKNVKRSVLFKLKSKSKNVFKKIGLKVDKTNIKAGVTGVWASFSIVAIFA
                     FVTSIMTIPQKAVPQDTTKYSIYSSKPLTIKTTTASIYSRDSRAQKINAVYKIYNCPL
                     EGMGDVMVYEADKNNIPWWLVAAISFQESGCGKKTPKTNGVESYNAWGWGVYGDYVFS
                     FDNWVRGIETVSKYLSDKFYSRGVTDTCEIMKIYTPPSKGSWCEGVNHFGDTIQNYET
                     PLN"
     gene            complement(11221..11568)
                     /locus_tag="P5014_00405"
     CDS             complement(11221..11568)
                     /locus_tag="P5014_00405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56606.1"
                     /translation="MFNKNENKEQYTVTLCSYKDDNNVLWTPKAIIRKKEGEKWEDYP
                     MQWGDETFFSRRKADDYALIKAKQWLSGNSGIKEKRIFTKEEWASHKLAVFLGLLFLV
                     PIILAIILGLIYS"
     gene            complement(11723..11929)
                     /locus_tag="P5014_00410"
     CDS             complement(11723..11929)
                     /locus_tag="P5014_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56607.1"
                     /translation="MDRLTGQRIRLTSFLLPPLSKEIFIKYGRYKQLKHWANWGFYGT
                     HSIQGAIELIERKVKEEEEKERKS"
     gene            12060..12305
                     /locus_tag="P5014_00415"
     CDS             12060..12305
                     /locus_tag="P5014_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56608.1"
                     /translation="MSNHISPEIVDLIMRIKPVKWKISKAEAINMNEDFLWFDDAPAL
                     EDIENLKKHHKLDSLIRVNLDENPDILKEFCKGSKAS"
     gene            complement(12292..13059)
                     /locus_tag="P5014_00420"
     CDS             complement(12292..13059)
                     /locus_tag="P5014_00420"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HRY56609.1"
                     /translation="MISSEQLKTFAKEYKINENIVAREFVQVTFLKELYEQRFSKEIF
                     FKGGTAIRLMYGGKRFSEDLDFTVTSNESEFLKKINVFFKQLSNKYPFTFKERETVAG
                     KTFLLTAGIPNLTSNIFVKLDFSMRENVINPVQEILKTEYPIIVTAFINCLSKNEIFA
                     EKIRAVMKREKQRDLYDLWVLYELGATPDLKLITDKLSYYGEKLDKKTLLDNLKKFKK
                     QEFIMDLKPFVTIDEREKLGDLFDYLNKYLEEQFKKL"
     gene            complement(13056..13646)
                     /locus_tag="P5014_00425"
     CDS             complement(13056..13646)
                     /locus_tag="P5014_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56610.1"
                     /translation="MSSLDNLNKLYKNNISLLTTLDIENLLQIKSSRTLEDLIKRLIQ
                     ENILTSVEKGKYYVTSKQPSNFEIANFLYSPSYISFETALNYYGLLSQFPLEITSATL
                     GKKTEKEINNQLYTYSKIDKKLFTGYYKESNYLIATKEKALFDEIYLISKSIKSESIL
                     QNLDLSNFEIDKFEEFLNLLPKKSLKAFQNIMEKYL"
BASE COUNT         3976 a         2587 c         2296 g         4949 t
ORIGIN      
        1 atctttcttt gctatagcaa tttctatcga accgaagtca gtttcaatgg ttgtttgtaa
       61 agcttcatat ccgttaattc ttttatttgc gcctatgaat tcatcaaatc tattgaaatc
      121 tactttaccg ccatatacat tatgtatcgc atatatggct tcataacact gctcaattga
      181 ttttactgca accctgtaac ttatgacgtc attaattttt ttataatctt cacttgaagc
      241 taaaccttta acagcgttag actttaattt ttggtaggta ctgtaataac ctccaatttt
      301 agtttcgaca ttagctttta ttttagtttt ttctagagcg tttcttattg ttttgaggtt
      361 tttttctatc tcagtgctag atagtctttt atcacaatca atagaagttt ttacttttga
      421 aaaattatca aaattgagat attgaaaaga aaggtcttca atttttgtct tgaccaccca
      481 cataccaaga gattctgcaa gttttgcgta tatttctaaa ctttctttag cttttttctt
      541 tcggtttgag tttggtaagt attgaagtgt actaaggttg tgatatctat cagctagttt
      601 taatactgaa acctttgggt caacgtaaga ggtgcctact atctttttta aagttttaaa
      661 atcctgtccc tcttttaccc ctaacctgat ttttgttacc gagtctacta gaaatgcaac
      721 ctcttctcca aagtattttg aaatttcttc aatctttaaa tttgtgtcct ctagtgtatc
      781 gtgtagcaga gctgcaatta ataagtttgt gtcgtttaca ccccattccc ctaatatgtt
      841 ataaactgct atgcagtgtt cgatgtacgg ttctccagat tttctgtata ggtttttgtg
      901 tttttcctga gcgattttca aagctgccaa aattttcctg tcagaggttt tcagattttt
      961 tttatattgt gtttcaaaat tttccgttag cattatcggt aataatatag ctaaataggt
     1021 tttattacaa cggagggggt ttttaaaatt ctgcagaata taaattatcc aattccctcc
     1081 attttattgt ttagattttc agatttaaag tattggagga ttagtaaatt attaaatttt
     1141 gaggtttgat gatttcctgt ctaaataaca gatagctggt gggaagagtg ggattcgaac
     1201 ccacgaaggc ataagccagc agttttacag actgctccat ttgtccactt tggtatcttc
     1261 ccaagaataa ctctaatgca ataacattat aacaaaaaaa ataccgaagc gagctgtttt
     1321 agaaatgagt caagaaaaat aaaaagataa ctttttatgt ctcttgtcac ttcataaaat
     1381 aagcttgaac ttcggtatat tgtaatgggg tgagtgagat tcgaacccac gaaggcataa
     1441 gccagcagtt ttacagactg ctccatttat ccactttggt atcaccccgg tcactagctt
     1501 gtagaacact atagcatatt ttgagtgttt gtccactcta gcctgtgtcc ttctaacgac
     1561 ctgtttctgc cttgattttt gcaattttat ctttgtactt taagaaactg aagctgctgc
     1621 tgtcaggaat tcctgaaatt ttcaacccag cttgtgctcg ttctatctcg gccaacatta
     1681 tctcgctggt ttttatagct aaacctacgt tgtagatgta gcttccacga agaccgtgaa
     1741 tctcagcctc ttttttgttc agacttacat gaggcatttg gattaattct tcacgtagac
     1801 tttgtgaata aattaatctt ccaattgtaa aggctgcaat ctcaatttct tcctcaactg
     1861 ttttttgagt tgagactgga cgaaattcta caatcgcttt gtctttaata aattttattc
     1921 gggcatcacg ccagaacaaa cctattccaa tttgcaaagc atgtgcttcg tcttcaagaa
     1981 taaacggata agaagacacc tccttcatgt aatcctcgat gctgttaaag tagttgttcg
     2041 gtaaacctac tcttagagat tttccttcgg caatgtcttg ttccgacctg gtgtcgtgcg
     2101 accgtttcca tgcctcaaac cttacatctt cccatccagt atcaaccccg ccaagaaatc
     2161 ttgcatttcc ggaaagtgct acagaaagtg ggctgatcat gtagagtctg tttagcttat
     2221 cgacagcgtc ttcaagacca ttacactgaa ctgagaactg gaaggcgtta aggataccta
     2281 caattgcggc atctaatacg ttaacaccat tgattgtttc ttcaatcttg cgcgtcttgt
     2341 tcaagttgtg aaaggtaggt acctttgtgt acttaggtgt tttagtcctt tgtatgtttt
     2401 gcgtatctgc ccaaggaatt gttccgtatc ttccaacttt gcagccgtgc ttttctacaa
     2461 caacccagag ttgtttttct aagtctctta gctgtgcgag ccatcccgag aatccgacct
     2521 tggttagatc aacaggcgct gggtgaacct caatgcatga agcaccgagt tccggtccaa
     2581 aatcgtcacc aagtttagca agcatttcgt ttttttcttc atctgtaata agctcacttt
     2641 taccttcaac ctcacggtag ataaaaagtt cttgctctgc tcctaccatg gtaatggtag
     2701 gtgttgggaa gccttttggc actttgctaa tttgctcttt tgacattttg ttaaatgcac
     2761 taagcatgtc catttcctta ctcctctcgt ttcacgtact aataattatt tatttttcaa
     2821 ggttccacaa acctgactta ctttctcttt aacttaccga ctgtaaataa ccttccgccg
     2881 gtagtaacaa actgcctatc cttcaaaacc ataagtaccc cactttcatg agatttaatt
     2941 tgcgtctttt tgaaagacgt aatatcaatc acccctccta ctacctctcc cttctttatt
     3001 ttatccatta gctttttgtg agtaacaaaa attcctgaaa cattggaatt aaattcttta
     3061 ttctctacaa ataacaactc cctgctttct ttagcatgcg aaacattagt tatttttaag
     3121 ttatttaaaa tctttattgt tccgtcaaca gctctttcta tctgctcatc acttacattg
     3181 tgtagctgag gaagctctac agcaatattc gggatacctt caaaagaaag tttaggaccc
     3241 aacgccgtga cgaatttaag ctcgtccctg gattttatgt ttaattccca aacaatatcc
     3301 ggatcaaaca acttgataaa ttctaatgtt tttttcttgg tttcattgga tccgcagttc
     3361 ataaagatat ttgtaacaac agtcgggtct tcaaaagtgt gaaaatctaa aactgcatcc
     3421 atgtctttgg caatctcaaa aatttcggct gcaattctat ctgttggttc ttttgtctta
     3481 tcccccggaa attttctgtt aaggtcgtac aagtccaaag gatttattct aatatttaaa
     3541 gcttgggcta aagcgttcgc agaaggaatt atccataact ccccctgaat atctttaatc
     3601 ttttccaaaa cattgtttat aacaagaagt cccgtgtact catcaccgtg aatcccgcac
     3661 acaataagcg ctttttttcc acgcttgctg cctgtaaatt ttaatacagg aatttctata
     3721 ctcactgccc ctgcttgtcc aatttttatt ttagttatag tttttttcat aattatttta
     3781 actttcttgc aaaaaaaagc ccttaggttt ttaccagatt tatctgataa acacctaagg
     3841 acgttaatac gtggtaccac cttagttttc tcaatcgtta ccgattggga ctttacaact
     3901 gctccgcgga tgcgacacag tctttcgctt taacgggcga atctcgttcc tgatttagtc
     3961 aagacagctc cggaaatgta tttcgacttt tcgttatgcg ctaggtttgc accaaccacc
     4021 tagtctctgt tgtcactaac ttatgtctac taaagtttcc atcaaagctt tatgtagagg
     4081 agtatagcta aattaacaca atatgtcaaa ctatttatta ctagttttac aatctaggaa
     4141 tcaataaaaa accggaagaa atttttactt tcttccggaa tggaaaactt atcaatcagt
     4201 tttattgtgt atgtatcttg tggtcttcga tgtttttgaa gataaagaat agtaccagcc
     4261 ctgccgcaag aaccaccagt gaaattatct gattggtagt gatagcaaaa ggctgttcgt
     4321 ttttacttac tagatttgtt atgatcatag taacaagcac agagatgggt acaacacctt
     4381 gcgctgcaag ccaaattggc tcggaggtca taacccctct ctctttaagc ctgccacact
     4441 cacgtcttcc ttccatgaaa acccagaacc ctatggcatt gcccgcgaca ccgatgatag
     4501 acatcagtag cagactgttt aaggacggga ccatagtata gtgggtagta aatatcatga
     4561 taggaacgat gaatactact gtagttgcct caatcacgaa tagagcaatc cagttatcgt
     4621 atccgtgatc aatgaagttg aacagcgcct gtgtgagagc tgcaattaaa cccataacca
     4681 gaatacgcgt ggtaaaggaa acgatagcat ccatctgata gaagactaca gcggccacag
     4741 tcagaatcac cgggatgatg aacttcgcac cggattttgt tacatagtaa gctttttttc
     4801 cgtacttgag aattgaaagc gcaataacca ttaaaggcca cgtgtagttt agaattgtca
     4861 tgcctacagg ctcaccttcg tagattgcct ggaagtagaa aagtgagtag attaaaggcc
     4921 acaccacgcc gattgcgacc attaagccgt aatccttgaa ggagtagttc ttaagttttg
     4981 tccaataagt cctcgccaca ccgttttgat ctttatatct cattatggtg atgatcagag
     5041 caagggcgat tagagctgtg acattaacgt aaaaggtagt aacgtctccg ggcatgttcg
     5101 cgagctttcc aaaagctgga actatacccc aggtgacaat caaaagtacg atgaagccta
     5161 gcaatcttcc ataattaggc tccttaccga aagtattttt tgagttcatg atagcctcct
     5221 aagctattgt attcagtttg ggatgatttt aactgctaac cactttaagg aagttagttt
     5281 ttccgtattt gatcaatacc ggtttgttga tttgctgttt gggatctgtg gctttctggt
     5341 cgtctatttt tactgcacct cctttgactc gtcgtgcgac ttcactgttg gacaccaaac
     5401 tgccagaagt aaccagaatc tcgtcaagac gcatgctgtt tgaaagcttg aattctggga
     5461 ttgaacctag gttttcactg cttccagtga acaccatcgt cgactgtttg cttgcatttt
     5521 tggcttcttc ttcaccgtga actacttttg taacttcaaa tgcgagtttt ttttgggcat
     5581 ctcttgggtt tccagccata atcttgtcta tctcttccat tggaatgaaa gtgtacaaac
     5641 cgaatagctt tcttaccatg tcgtcgggga ttttctcaat gtattgatag aaatcgaacg
     5701 ggctggtttt ttttggatca agccataaag gtccacttac tgttttaccc atcttttcac
     5761 cgtttgcagt ggttagaaga gggaatgtaa gggcgtgcgc attggctttg ttacccttgt
     5821 ggtcttttat aagactcaca ccttgcagaa tattacccca ttgatcactt ccgcctacct
     5881 gaagtctgca gtcatatttc tcataaagtt cgaggtagtc ccaggactgc attgtggcat
     5941 atagaaattc caataacgat aggggtgccc cttccgaaag acgtttggca aatgtttcca
     6001 tcttaaccaa ttcgtttaca gaaaagtacc tggcgacgtt catcatatag tcgtccagga
     6061 agagtgcttt ggcgagccag tcggagttat caatcatcag agctttcttt ggattatcca
     6121 aatccagaag tcccatactc tgaacctgat ttttgatcgc agtagcgttc tttattacca
     6181 tttccttcgt tagaagggat ctggccttgt ttttgccact gggatcgccg actctgcctg
     6241 taccgccacc aagaatgaaa atgatcctgt tcccagcttc ctgcatgtga tgcaaggcca
     6301 taatactcat aaaatggcct aggtgaaggc tgtctgccga agggtcaaaa ccctcataaa
     6361 cggttgtacc ctgggttaac aggttactga cagcttcttc gtcggttact tggaattttt
     6421 tgataccctg ctcttcaaca gttgcgatgt aaccacgttc ttttagtaat tcataaatat
     6481 ttctcatatt aacctctctt atatttgtac catattttat tatcaaagat ccagtccttt
     6541 gactttggtg gattgtacga taatataagc ctgtagtcaa atccctccta gttataccaa
     6601 acttattgaa atgctgacat agctttagat gtagagttat ttataacaac ctagaagttt
     6661 gaatgagcta ttccttcata tatatttggt ttatatagat gaaggagctt ccttgctaaa
     6721 gatctttctt tcttaggtcg gatgggtttt gttataccta aaaattattc aatagataaa
     6781 gtttttatgt aagcttcaaa tacctcattt gttttaatta cactatcaat atcaacgtat
     6841 tcattttctt tatgtgcttg atcagatggt cctggaccaa agactaaaat atcagatccc
     6901 cagctttctt gtaatagctg aacttcaaaa tatcctgtgg agtctatatc cttgtaaata
     6961 ggttttattc cgctttgttt aacaatttct tcgaagcctt ctaatttact tttaggcgtg
     7021 taagcaggtt tcattagaaa cttgaattta atttcactaa ctttgattcc gagctcttta
     7081 gcgttagttt ctattttatc ctcaatccat tttcttgata ttctcttatc ggaaagtctt
     7141 atttcactca cacaaaaggc atagtcagga acgacgtttc cactataaga gatacttccg
     7201 tcatttttta agataccccc atttagatga gccaaattta aagagttatt tccaccttca
     7261 ttatcatatt tacttatgtc cttttggagc atttcaaaca ttgttattgc tttctctata
     7321 gcatttattc ctaaatactt tgtacctgcg tgacacgact ttccatgaac attgaaagag
     7381 aattcagtta ctcctctaca accgtttgat atctccaatg aggtgggttc cggattaata
     7441 atgatatctg gtttaaattg atatttttcc aataacttta atgccccttt aaacgcatac
     7501 tcttcgtcaa ctgtaaatat ataagcaatg ttttttgatg acttaaattt ttttgctaaa
     7561 aatagacaaa ccgataatcc tgctttcata tcaacagagc ctaacccata tatcttgttg
     7621 ccagattttt ttgctttgaa tggttcaggt gtactaattt tgggaggcac tgtatccatg
     7681 tgtgaaaaaa atattatgct tggtttttta ttattgtatg caattatatt aaatcgttta
     7741 ccttcgacag tttgtttttc aacttttaga tccgtatttt ctataaggaa tttgtatata
     7801 aattctgcga aaatagattc atctacaccg tcaccaagat acgacggtat tccaattaat
     7861 tttttaagta actctgtgac ctctttatcc atgttataga ttatacagac aaaattcgat
     7921 acgcaggtaa tgtttatcac atttattgtt atagcaacaa atacaaggtt ttggtgggcc
     7981 atttgtgaag aagtgcgaac tgctagttat tattcaacaa actcaagtta aaccagtttt
     8041 aagatttatt gataaaatag aatatatgat gatcaactat cctttaaagg agggcaagat
     8101 aattcaacca tttggtagag atgcctctga tgacccaata tataaggaat tttacaaaat
     8161 ctttgattat aaacattgtg gtatagattt cgatactgaa ataggtacta atgtttatgc
     8221 agctttctcc ggcattgtcg ttagagcaga aaaccacgaa ggtatgggaa atgttatagg
     8281 gattagaaac ggaaaccttg tagggttata tgcacatcta tctgaaatag gtgtaatact
     8341 tggtcaatta gtatctacag gtgatctgat aggaaaatcc ggagcgactg gaaaagcatg
     8401 ccccaagcca caccttcatt ttgagttaag agacttaaca aaatcttcat taaaagaaat
     8461 ggtatttgaa cctatttttg aaaaagaaat agatcgatat aaagacactt ttacatacac
     8521 tgtaaataat aaaaatacac aaaagacatt aaggtcatta tccaaacttt attttggtac
     8581 ccagaattat tgggaattaa taaaaatagc caacggattt gattttgatg agagtattgt
     8641 attagaagag gctagtgaaa taatcattcc aaattataat taaatatgat tgaatttcgt
     8701 aaaaagttcg agtccttctt gggttagtca taagtgtggt tcttataaca acaaaaaaac
     8761 gtggtgggcg agagaggact cggtcacgcg tcttaacatc ttgtacctcg ctatcgctca
     8821 aacaatatgt aaagcccgcg accccgtctc gctcacttct ttcgctccga cattctcgtc
     8881 ctctctccta ataaaagttc ccgctaacct ctcagaaatt caaggctaac gggaactttc
     8941 atggtgggcg agagaggact cgaacctcca ccctttcgga cacggtccta agccgtgcgc
     9001 gtatgccaat tccgccactc gcccatgatt tattttctat taaattttta aatttacttt
     9061 tatcttctgc acgtcttacg ccctctaatg gtctaaagcc gtgcgcgtat gccaattccg
     9121 ccactcgccc atgatttatt ttctattaaa tttttaaatt tactttcatc tttcgcacgt
     9181 cttacgccct ctaatggtct aaagccgtgc gcgtatgcca attctttact actgtaaata
     9241 tgtttgctta cgcaaccata aagccactcg cccatgattt attttctatt aaatttttaa
     9301 atttactttc atcttccgca cgtcttacgc tcttaaggga tctaaagccg tgcgcgtatg
     9361 ccaattctct actactgtaa atatgtttgt ttacgcaacc ataaagccac tcgcccatgg
     9421 gtatttagta acttctttta cgaacaaaac aattatagca gaaacaaaaa gaagcccgcc
     9481 ttcggcgggc ttcccactat tatttcacaa ttattccaca gttattgaaa taatatttac
     9541 ttatagatta atcttgagtc atatctagaa atttttgatt tcaattccct ttatattatt
     9601 ggatttttac cccagttgcg ctgagttttt taagaagcaa ataaaatact tctatctatc
     9661 aacttaggat tctcaatttt actaataaaa aagttaacac cgtttctcca gttaattgaa
     9721 tttggaggtg tgtacacagg agcaattttt tcaggagtat ctaatccttt agctacatat
     9781 ttttcagata ttccctttcc cactgtttca atagcttctt caaatgattt aaaataaata
     9841 gcatttttac catagatacc ccatccaaaa gggttgtacg aattaggcaa catcctttta
     9901 ccacatgaag attccataca tgatattgct ggtaaaagtc tgtaatccaa accatatttt
     9961 tccgctactc taacaaacgt atcggcgtcc tcttttaaag gtgatttttg agcttcgaag
    10021 aacttttcta aagattcaat cttctcttct ctacccgtat ctatttcctt tccgataaag
    10081 ctcatagaag catctaaggg cctgttattc tctacagtta caagaatagc catccacaca
    10141 aagatagcga caaaaggtaa tatttttagt tgttttttca gtattttttt actcatacaa
    10201 ggggtgattt tagcacattt ttcaattatg tgcaaatgct ataggatata tttcataagg
    10261 ttagtagtta taaaaagtta attcaaaggg gtttcgtagt tttgaattgt atccccgaaa
    10321 tggtttactc cttcacacca cgatcctttc gatggcgggg tgtatatttt cataatttca
    10381 caagtgtccg tcacacctct tgaataaaat ttatcagata aatattttga aacagtttca
    10441 attcctctca cccaattatc aaatgaaaag acgtaatccc cgtagactcc ccagccccag
    10501 gcgttgtaag attcaacacc attggtttta ggtgtctttt ttccgcaacc tgattcttgg
    10561 aatgatattg ctgctactaa ccaccaagga atgttgtttt tatcagcttc gtaaaccatt
    10621 acatctccca taccttctaa tggacagtta tatattttgt aaacagcatt tattttttga
    10681 gctcttgaat cccttgaata tattgaagcc gtagttgttt ttattgttag aggttttgat
    10741 gagtatattg aatattttgt tgtgtcctgt ggtactgctt tttgaggaat agtcattata
    10801 gatgtaacaa aggcaaatat ggctacaatt gaaaaagatg cccatactcc ggtaactcca
    10861 gctttaatat ttgttttatc tacttttaag cctatctttt tgaaaacatt cttggattta
    10921 ctctttagtt taaaaaggac acttctcttt acatttttag tatcttcaaa tcctcctgca
    10981 attgaaaata tattctcatt tattaaagca gaactaagag agtttatctc ttctttacta
    11041 taaaaattgg agattttctt tttgaggatt ttttttccgt ttttataaaa acagttattt
    11101 gaatttactc ggttcatatt gattgagata cattatcaca agatactgaa aatttacaag
    11161 atgacaattc tactgatctt tgtttaataa ttgaaaacat attttgaaga ttatattaat
    11221 ttatgaatat attagaccaa ggataattgc aagaattata ggaactaaaa aaagtaatcc
    11281 taagaatacc gctaacttat gtgatgccca ctcttcctta gtgaatattc ttttttcttt
    11341 tatacctgaa ttacctgata accattgttt agcttttatt aaggcataat catcggcttt
    11401 tcttcttgaa aagaaagttt catcacccca ctgcatcgga taatcttccc atttctcgcc
    11461 ttcttttttt ctgataatag ccttgggagt ccacaacacg ttattatcat ctttataaga
    11521 gcagagtgtt acggtgtatt gctctttatt ttcattttta ttaaacattt attattttgt
    11581 gaatttgcaa taaatacgaa gtaatgtatt agttttatct taacataatt aacgttttgt
    11641 atttcaaaaa ttgggataga ttattgaagc tgggttttta agaaaatcta cttaaaaaat
    11701 taaggagcgc ttgtttaata aattaacttt tacgctcctt ttcttcctct tcttttacct
    11761 ttctttctat aagttctatt gcaccttgaa tgctgtgagt accgtagaaa ccccagttag
    11821 cccaatgctt gagttgtttg tatctaccgt atttaatgaa aatttccttg gatagcggtg
    11881 gtaataaaaa actggtaagt ctgattctct gtcctgtaag tctatccatt ttctcctcct
    11941 tttgtatttt ttattgagta tacaggattt ttttgtttgc gggtttgtta agtaaaaaat
    12001 tacgatgttt attggtttac taccaaatgt gacggagatg ctacatcagc agattttata
    12061 tgagtaatca tatttctcct gaaattgttg atttaataat gaggataaag ccagtaaagt
    12121 ggaaaatttc aaaagcagaa gctataaaca tgaatgaaga ttttctttgg tttgatgatg
    12181 ctcctgcttt ggaagatatt gaaaatttga aaaagcacca caagctggat tctttgataa
    12241 gggtaaattt agatgagaat ccggatattt taaaggaatt ttgtaaaggc tctaaagctt
    12301 cttaaactgc tcttctagat atttatttaa gtaatcaaac aagtctccaa gtttttctct
    12361 ttcgtctatt gttacaaagg gttttaaatc cattataaat tcttgctttt taaacttttt
    12421 aagattatcc aaaagagtct tcttatctaa tttttctccg tagtacgata atttgtcagt
    12481 gattaatttt aaatcaggag ttgctcctag ttcatataat acccaaaggt catacaaatc
    12541 tctttgtttt tctcttttca ttacagctct aatcttttct gcaaatattt cgtttttgct
    12601 taaacaatta ataaatgctg taacaataat gggatattca gtttttaaaa tttcttgaac
    12661 aggattaata acgttttctc tcattgaaaa atctaatttt acaaatatgt tggaggttaa
    12721 attaggaatc cctgcggtta aaaggaaagt ttttcctgct acagtttctc tttctttaaa
    12781 tgtgaaagga tacttgttgg aaagttgttt gaagaataca tttatctttt ttaaaaattc
    12841 agattcgttt gaagttactg taaaatctaa atcttctgag aatctcttac caccatacat
    12901 aagtctaata gcagtcccac ctttaaaaaa tatttctttt gaaaatcttt gctcgtataa
    12961 ctcttttagg aatgtaactt gaacaaattc tctagctacg atgttttcgt tgattttata
    13021 ttcttttgca aatgttttta gttgttcaga tgaaatcata agtatttttc cataatattt
    13081 tgaaatgctt taagtgattt tttaggaaga aggtttaaaa attcctcaaa tttgtctatt
    13141 tcaaagtttg aaagatctaa gttttgtaaa atgctctctg atttaataga tttacttatt
    13201 aaatatattt catcgaatag tgccttttct tttgttgcta ttaagtagtt tgattctttg
    13261 tagtatccgg taaaaagttt cttatctatt ttagaatatg tatacagctg attatttatt
    13321 tctttttcag ttttttttcc aagtgtggct gaagttattt ctaagggaaa ttgacttaat
    13381 aaaccataat aatttaaagc ggtttcaaat gatatgtatg aaggtgagta caagaaattt
    13441 gctatttcaa agttagaagg ttgttttgat gttacataat attttccttt ttctactgat
    13501 gttaggatgt tttcttgaat tagtcttttt attaaatctt ctagtgttct agaggattta
    13561 atttgcaaaa gattttcaat atctaacgtg gttagtaagg aaatgttgtt tttatatagt
    13621 ttatttaaat tatctaacga tgacatatac cacgtattat acgtggtttg tggtatcgtg
    13681 tcaattagta taagttgcat ttataaagta cattttacct gaaaaaatga agcgaaaatg
    13741 aaaaaaatca ggcgtttgtg gaaacttaag cacactgttt agggtgtggg tcggaatccg
    13801 gcctgatt
//
LOCUS       DAOTFA010000005        13795 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate AMR_MDS_3115
            NODE_3074_length_13795_cov_8.986317, whole genome shotgun sequence.
ACCESSION   DAOTFA010000005 DAOTFA010000000
VERSION     DAOTFA010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868139
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (wastewater metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 13795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:36:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 536
            CDSs (total)                      :: 521
            Genes (coding)                    :: 520
            CDSs (with protein)               :: 520
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13795
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3074_length_13795_cov_8.986317"
                     /isolate="AMR_MDS_3115"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..194)
                     /locus_tag="P5014_00430"
     CDS             complement(<1..194)
                     /locus_tag="P5014_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56611.1"
                     /translation="MVYIVHGEDISKSRILIQNQQKKIGAQSRIEIEISEITPENLYE
                     KGCSNDLFGNPPFIVLDITNA"
     gene            complement(246..1391)
                     /locus_tag="P5014_00435"
     CDS             complement(246..1391)
                     /locus_tag="P5014_00435"
                     /inference="COORDINATES: protein motif:HMM:NF015755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HRY56612.1"
                     /translation="MSKQNKMAARRKGRGLSKVFVFFIALAIISGIFLVFKDTVKKAF
                     SPVSIVGGSTKIDIKETDGRTNILILGSDKRNFGNESGRNTLTDTILIASIGNTDNDV
                     VLISLPRDLWISNYNMENGYKYSSKINEVYSNAGIQELRNQLEVVLGIPMHYHVIVTF
                     DIFENIINILGGIDINVETSFTDYAYPIEGKEGDLCGKTQEETDKIFKEAEEKELDKE
                     AVALNTFPCRYETLKFEQGLQKMDGKTALKYARSRHGNNGESSDFARSKRQQNVIMAV
                     KNKGLSLETLVNFSKLKELYDQYANNVETDIKLGDLQSFFQLSQKLDFDKVIQIVIDD
                     SADGNEGGLLYHPNDSTLYGGAWVLIPQAGDYSQIHAYVQKYLFGNK"
     gene            complement(1461..2159)
                     /locus_tag="P5014_00440"
     CDS             complement(1461..2159)
                     /locus_tag="P5014_00440"
                     /inference="COORDINATES: protein motif:HMM:NF012349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HRY56613.1"
                     /translation="MKYVIANWKLNMDSKDLSNWVGPFSKKYQNIKKDWKKRRIIEER
                     WPKILLSPSLVYIPAVTEISQKMGVESCAQDVSPFERGAFTGENGAFQIKVFSKYAII
                     GHSERKEPVEVVARKRDLCLMEKITPIVCFVNPSDLLRLYKDGCVMAWEDPQNISING
                     VYRSEDPAKISAIAKEIRKIVPPEAKLIYGGSVNERNVAAISRISELDGVLVGNASLD
                     PEIFATIVRYFVQD"
     gene            complement(2156..3004)
                     /locus_tag="P5014_00445"
     CDS             complement(2156..3004)
                     /locus_tag="P5014_00445"
                     /inference="COORDINATES: protein motif:HMM:NF014750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HRY56614.1"
                     /translation="MIDISVKKVLSIDLEKQEADVKSFIDLNKYFGGTGLGLKFLEIY
                     KDKNPLIFSVGPLNGFFPYTSKTSIVLEKDSAVEDIYIGGSLSTRIKFAGIDSVVLFG
                     VSKEETILDIQNSVVNFKNKDTEPQSLGLPGKRSGIYFEGDKLLCDQYFTTPEYYLET
                     ILKEKNIKGITITGTEIFSPKNFDDYKKLYLEILDRKLDLRVDPADYPSCSGCPVGCG
                     KSKVGEIGGNVLLNSLVACQFADKIYTDIGIVFSCLNTLGYEYTHEDIENLPGLIEET
                     IRNISK"
     gene            complement(2997..3257)
                     /locus_tag="P5014_00450"
     CDS             complement(2997..3257)
                     /locus_tag="P5014_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56615.1"
                     /translation="MTKIIKPTYINRCNGCELCIFEAQRQLKKVGLEDSLIRVFRKKN
                     PETQALGFELEIDPRINILDIEKIELICPKKVFEVEEDTKND"
     gene            complement(3271..4242)
                     /locus_tag="P5014_00455"
     CDS             complement(3271..4242)
                     /locus_tag="P5014_00455"
                     /inference="COORDINATES: protein motif:HMM:NF012389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HRY56616.1"
                     /translation="MKLLIDAPINKGTRVFVRCDLDVPVENGVVKEAFRLDSSLPTLK
                     YIIEKGGIPVIAGHMGKPDGKVDLNLSTQQLVPYFNENLGEGAYEILENLRFNPGEEE
                     NSEEFSKELASKAEIFVNESFATCHRKHASIVGITAFLPSFAGFNLQKEVEALTKVME
                     NPEKPLALIIGGIKLESKMPVIEKFFDKADFIMLSSILSANWSKEITHNMLLADNKDV
                     ESKDINENTRQKFIWAIEKSRTILWAGPLGMYEDDQFIQGTKEIAEKIAEVTQKDGVY
                     SVVGGGDTVAAINKLGLMDKFSFVSTGGGAMLQFLAEGTLPGIEVLK"
     gene            complement(4284..>4490)
                     /locus_tag="P5014_00460"
     CDS             complement(4284..>4490)
                     /locus_tag="P5014_00460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008579150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HRY56617.1"
                     /translation="VKEEKYKGVVRATFEPLVSSDIIGDPYSAIVDLSMTKVIDGNLI
                     KVFAWYDNEWGYSCRLVEMALMML"
     assembly_gap    4491..4590
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4591..5228)
                     /locus_tag="P5014_00465"
     CDS             complement(<4591..5228)
                     /locus_tag="P5014_00465"
                     /inference="COORDINATES: protein motif:HMM:NF012274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HRY56618.1"
                     /translation="MENKTARIAINGFGRIGRSSFKIAFDKGLDIVAINDLTNPLVLA
                     HLLKHDTIYGVYDKEIWVEIDGKRINLEDNTGEKSFFDTKGTENYIVVEGKKSLVTAE
                     KEPEKLPWKDLDVDVVLECTGRFVKDDTSLAHIKAGAKNVIISAPVKGGTIQTFMKGV
                     NDSQYLGQNAISNASCTTNCISPVLAILDSNFKILKAGMSTIHAITNNQNVA"
     gene            complement(5243..5509)
                     /gene="trxA"
                     /locus_tag="P5014_00470"
     CDS             complement(5243..5509)
                     /gene="trxA"
                     /locus_tag="P5014_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009384495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HRY56619.1"
                     /translation="MIKLLDFYADWCGPCKMMAPVFEEMKPEYAGKVEFQKIDVESDS
                     STAANFGVMSIPTFVLVQDDKEIDRKVGAMPKEMLKSWIDSHLK"
     gene            5588..6565
                     /locus_tag="P5014_00475"
     CDS             5588..6565
                     /locus_tag="P5014_00475"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HRY56620.1"
                     /translation="MQDLPDKKLKLLPVSLIIALVFGVGVFAFRKLFTREIVIKEDLS
                     EKKYYTSLLRSENLGSEWEPGEMLEVNLEDQPEISAESGLVVDINSGKIVFEKNSKEK
                     MKIASLVKIMTAVVALEHKNLDERIVVSKKSAGVGENTMGISEGEVYTLEELLYGLML
                     NSGNDSAYAISEGVAGNAEDFVDWMNIKAKELGLNDTEFFCPSGLSDETYSTAVDLVK
                     LSRYALQNEDFRRIVGTVDFELPYSERHKYLYLENQTNLLTTYPGVGGIKTGYTEEAG
                     YCLVTYAKNEGVELIGVVLNSVNRKFDMLNLLNFGFGVYGIEVESGYEL"
     gene            6602..7201
                     /locus_tag="P5014_00480"
     CDS             6602..7201
                     /locus_tag="P5014_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56621.1"
                     /translation="MENNGKNNNILPLVVIFLVIAGLIGGAYWFGKSSIMQNSQTGQM
                     TDVKESTEPENKSLQEPEKSTTEPSENVELEGETGAGETEDKPTEEGPAVEEDSDESV
                     IESLKILFAQKYEKRIEDITVTISKREGNYLTGGVKFTAGGEGGYVLAAKVNGAWKIV
                     FDGNGSWTCSAVDVVDFPSTLAPECWDEDTMAVVDRTAN"
     gene            complement(7279..7539)
                     /locus_tag="P5014_00485"
     CDS             complement(7279..7539)
                     /locus_tag="P5014_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56622.1"
                     /translation="MKNLICFFTGFIFGKLEVAAYNCFPKTGRRATHFGKYHFHHSIQ
                     GVLLIIVGKVLGGNTGKYVSYFGLGILTQHTADEGLVFITKD"
     gene            7667..9622
                     /locus_tag="P5014_00490"
     CDS             7667..9622
                     /locus_tag="P5014_00490"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HRY56623.1"
                     /translation="MPTNSELFTGLNPQQIDAVKHLDGPALVVAGPGSGKTRVLTHRV
                     ANLIQNHEVSETNILCVTFTNKAADEIKQRVGAILSDSSGISKLPWSGTFHSICSKIL
                     RKDGYNIGIPPSFVIYDTEDSASVIKIILKDFGIDAKRFHPRGVLETISSAKSELITP
                     SDYESYAQGYYQRTVSKIYPEYQKRLQNSNALDFDDLLYFTVKLLDTDKKTLKKYQEQ
                     FKYILVDEYQDTNKAQYVLTRQLSDLHQNLFVVGDMSQAIYSFRGADFRNILNFQKDY
                     PEASIYNLEQNYRSTQVILDAAKNMIKKNSTHIPLDLWTDKEGGDKIVVFSGSTGNEE
                     TAFVIDEISNQMKLGKTFVDMAVLYRTNAQSRAIEEALIKNSIPYRVFGGVRFYSRKE
                     IKDIVSYLRVIQNPKDSVSWSRIINVPTRGMGQKSADKLKNADWDLDEIEKVSKIPIK
                     RWISKKDDMSTLELMEEVLKDTGYIKWLDDGTEEALSRIENIKELKTVASQFVNLSEF
                     LENVSLIEDTGKASEKNNVDCVTLLTAHASKGLEFPIVFIIGMEEGLFPHSNSLGEQE
                     ELEEERRLCYVAITRAKEKVYMTNARSRMYFGNIQANLPSRFLSEIPQELLDFRGLGI
                     KNYFPRSNKNDDFLDDLEFNRGNFKWD"
     gene            9645..10412
                     /gene="rsmA"
                     /locus_tag="P5014_00495"
     CDS             9645..10412
                     /gene="rsmA"
                     /locus_tag="P5014_00495"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HRY56624.1"
                     /translation="MFKPHKELGQNFLTDKSVAKDMVEALDIQNGDEIVEIGPGHGVV
                     TEFLVEKIKYLNVKIHAVELDVRLYQELKNFYSGNEKIEIIKENILDWLPTFSTDREI
                     KIIGSVPYYITSPIIHKIIKMPAVPQVVVLLVQKEVAEKIKSKAPDSSYMSSFVQTFF
                     DVKYLGKVPSKKFNPEPKVDGGIIKMVKKEDANLDPVFIRKYEGFLHKAYSNPRKMLN
                     KVFRKEELENGNINPTLRAQNLDAEEWLSFYKKINEI"
     gene            10402..11136
                     /locus_tag="P5014_00500"
     CDS             10402..11136
                     /locus_tag="P5014_00500"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRY56625.1"
                     /translation="MKFSICIPTLNEEKYIGGILNCLEKQTFKDFEVTVVDGNSQDRT
                     KEEVLKFSSKYPLQFLKSPQRGVSFQRNYAEKKSSGDYVIFFDADVMIESDFLEKISN
                     HLEKGDIDVLTTWNKPISDKPIDEFIYLFVNIFMLELIKKKNPGAVGVFICVKKSSFE
                     KIGGFREKVNFGEDFDLAKRLHDSGFTYALLKKPKVHVSVRRFDKEGRLNMIIKNLRA
                     ATYYLTNNNNYIDKIQGKFKHEFGKF"
     gene            11357..12292
                     /locus_tag="P5014_00505"
     CDS             11357..12292
                     /locus_tag="P5014_00505"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HRY56626.1"
                     /translation="MKRHILQSKLWEKFKNDYGTPAVRVGKILYTKHKIPFTNNYYGY
                     CPRVNPFDIDFEELKKSLNENNCVAVHFDVPNVTKDDPEAEKAEQILKEKCVLSQRDE
                     FAKGNFLMSLKKSEEELLANMHKKHRYNIGLAQRKGVTVRETTADEDFQIFYDLYFET
                     TQRQKFFGRSKTYLNGVWKTFKSANSAHLLIAEYEGKPLSSWMLLVQDGVLYYPYGGS
                     TEKDKDVQSNCLLGWEAIKFGKKLGCDLFDMWGAAEDMSNKSDPYYGFSLFKEKFGAT
                     HVSYIKSYDYVINEPLYKMFLVANNLRWKFLSILK"
     gene            12265..13191
                     /locus_tag="P5014_00510"
     CDS             12265..13191
                     /locus_tag="P5014_00510"
                     /inference="COORDINATES: protein motif:HMM:NF014445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HRY56627.1"
                     /translation="MEVFEYTKMKPELDIRQSDKWAKYLGRMGWESHRTSSGINIYFH
                     RTFLGTIIKIQKPLSVSKKDIKEIEDFCVKLKPLFIKIEPFIGQEIAVLEECGYVKSE
                     VALTPPSTIYIDLTKSENELWESISHSGKYSIKRAQREGTVTRFYQNPIKDKMGAYFE
                     MVQVTGKRKHFYVQPMVDFEEKVKIFGKECHLVLSYDTEGKLLSGKFYLCHRDMVLYS
                     TGGTSEKGLKTKAGYELLWKSILYFKGLGYKVLDLEGRDDPRFKDATKNWGGFSHFKE
                     KFGGENIEFPHPYIKYFNPVLKFFSKFMKLPL"
     gene            complement(13210..>13795)
                     /locus_tag="P5014_00515"
     CDS             complement(13210..>13795)
                     /locus_tag="P5014_00515"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HRY56628.1"
                     /translation="EFHSKIMSGNKPSLEDFYKIFEKNWNPLGYENEKDRKNSFEEGK
                     NILKDYYEKNKGLDAKHLGLEKKFVLSIDGTKLKGTIDRIDKLPDGTVEIIDYKTGKE
                     KSQKDVDDNVQMTIYTMGATEALKINPDVLSLYFLNTGNKISTKRTQKQVDAQREIIK
                     DVIKNINEENFEPNPGRDCMYCDFKEICPFAKKE"
BASE COUNT         4280 a         2340 c         2516 g         4559 t
ORIGIN      
        1 gcgttagtta tatcaagcac aataaaaggc ggatttccaa aaagatcgtt ggaacaacct
       61 ttctcatata aattttcagg agttatttct gatatttcaa tttcaattct ggattgcgcc
      121 ccaatctttt tctgttggtt ctgtattaat attcttgatt ttgaaatatc ttctccgtga
      181 actatgtata ccatgggttt attttaacta aaaaaatcgg atttacctat acgatttaat
      241 ggagattatt tgtttccgaa tagatatttt tgtacgtagg catgtatttg tgaataatcc
      301 ccagcttgag gtatgagaac ccaagcccct ccgtatagag tagagtcgtt aggatgataa
      361 agtagtccac cctcgtttcc atcagcgctg tcgtcaatga ctatctgaat cactttatca
      421 aaatctaatt tttgtgaaag ttgaaaaaag ctttgaaggt cacctagttt gatatctgtt
      481 tcaacattgt tggcatactg gtcatagagc tcctttagtt ttgaaaaatt aactaaagtt
      541 tccagcgaca accccttatt tttaactgcc ataataacat tttgctgtct cttggatctt
      601 gcaaagtcgg aactttcccc gttgttgccg tgtctagatc gtgcgtattt aagagcagtt
      661 ttcccatcca ttttttgtaa accttgttca aatttaagag tttcatatct gcaaggaaat
      721 gtatttaatg ccacggcttc tttatcaagt tctttttctt cggcttcttt aaatatttta
      781 tctgtttctt cttgagtttt cccacacaaa tccccttcct ttccttctat tgggtacgca
      841 taatccgtga aagatgtttc aacattgata tctattcctc ctaagatgtt tattatattt
      901 tcaaaaatat caaacgtgac tattacatgg tagtgcatag gtattcccag aactacctcc
      961 agctgattcc ttagttcttg aattcctgcg tttgagtaaa cctcgttaat tttggaggag
     1021 tatttgtatc cgttttccat gttgtagttg ctgatccaaa gatctcgagg cagagatatt
     1081 aaaacaacat cgttatcggt atttccgata gaagctatca gtattgtatc cgtaagtgtg
     1141 tttcttccgc tttcatttcc gaaatttctt ttatctgatc ctagaattaa tatgtttgtt
     1201 ctaccgtcag tttcctttat atctattttg gtggaacccc caactatgga aacaggggaa
     1261 aaggcttttt tcacggtatc tttgaagaca aggaaaattc ctgaaatgat tgccagtgcg
     1321 ataaaaaata caaatacttt agacaaaccc ctaccttttc ttcttgcagc cattttattc
     1381 tgttttgaca tatctgtgta tttcattttg atttagaatt cttaagaagt tattctacca
     1441 ccatttttca taagagatct ctaatcttgg acgaagtatc ttactatcgt agcaaaaatt
     1501 tccgggtcca aacttgcgtt accaactaaa accccatcta actcactaat tcttgatatt
     1561 gctgcgacgt ttcgttcatt taccgaaccc ccgtatatta attttgcctc tggaggcact
     1621 atttttctta tttctttagc gatagcggaa atttttgcag ggtcctccga tctatatacc
     1681 ccgtttatcg aaatattttg aggatcttcc caagccatta cacacccgtc tttataaagt
     1741 cttaataagt cagaaggatt aacaaaacat actatcgggg taattttttc catcaaacac
     1801 aagtctcttt ttcttgcaac aacctcaacg ggttcttttc tttcgctgtg cccaataata
     1861 gcgtatttac taaatacttt tatttgaaaa gcaccgtttt ctccggtgaa agcacctctt
     1921 tcaaaagggg atacatcttg agcgcacgat tcaaccccca tcttctgaga tatttcagtg
     1981 actgcaggaa tgtacaccag tgaaggggaa agaagtattt tgggccatct ttcttcgatt
     2041 attcttcttt ttttccagtc ttttttaata ttttgatatt ttttactgaa aggtccgacc
     2101 caatttgata ggtctttaga atccatgttt agtttccaat ttgctattac gtatttcatt
     2161 tagatatgtt tctaattgtc tcctcaatca accctgggag gttttcaatg tcttcgtggg
     2221 tgtattcata acccaaggta tttaaacatg agaaaacaat cccaatgtct gtatatattt
     2281 tatctgcaaa ttggcaagct acaagactgt tcaacaaaac gttccctcct atttctccga
     2341 cttttgattt accacatccc acaggacatc ctgaacaaga cgggtaatca gccgggtcaa
     2401 ctcttaaatc aagttttcta tcaagaattt ctagatatag tttcttataa tcatcaaaat
     2461 tttttgggct aaagatttcg gtccctgtta ttgtaattcc tttaatattt ttttctttta
     2521 aaattgtttc taggtaatat tccggagtag tgaagtattg atcgcacaat agtttatccc
     2581 cttcaaaata aatcccggat cttttacccg gaagccctaa tgactggggt tctgtatctt
     2641 tgtttttaaa attaactacc gagttctgta tatcaagaat tgtttcttct ttagaaacac
     2701 caaatagaac cactgaatct attccagcaa actttattct ggtagaaagg ctgcccccga
     2761 tgtaaatatc ttcaacagcg ctatcttttt ctaacactat ggacgttttt gaagtataag
     2821 gaaaaaaacc attcaaagga cctaccgaga aaattaaggg gtttttgtct ttatatatct
     2881 ccagaaactt taatcccaac ccggttcctc cgaagtattt attaaggtct ataaagcttt
     2941 taacatccgc ttcctgtttt tccaagtcta ttgaaagcac ttttttaact gatatgtcaa
     3001 tcatttttcg tatcttcctc aacctcaaaa actttcttag ggcatattaa ctcaatcttt
     3061 tcaatatcta agatattaat cctcggatct atttctaatt caaaccctaa agcttgagtt
     3121 tcgggatttt ttttcctgaa cactctgatt aaagaatcct cgagaccaac ctttttcagc
     3181 tgtctctgag cttcaaaaat acacaattca cacccgttgc acctatttat atatgttggt
     3241 tttattattt ttgtcattta ctcttagttc ctattttaat acttcaattc caggtaacgt
     3301 tccttcagcc aaaaactgaa gcattgcacc accgccggta gatacaaaac taaatttgtc
     3361 cattaatccc agtttgttta ttgctgcaac agtatccccg cccccgacga cagagtagac
     3421 cccgtccttt tgagtgacct ccgcaatttt ttcagcaatt tccttggttc cttggatgaa
     3481 ctgatcgtct tcatacatcc ctaaaggtcc ggcccacaag attgttcgtg atttttcgat
     3541 tgcccagatg aatttttgtc tggtattttc attaatatct ttagattcaa cgtctttatt
     3601 gtcagctaat aacatattat gagtaatttc tttactccag ttggcagata atattgagct
     3661 aagcataatg aagtcggctt tatcaaaaaa tttctcaatc acaggcattt tactttcaag
     3721 ttttattccc ccgataataa gagcaagtgg tttttcgggg ttttccatta cttttgttaa
     3781 agcttccact tccttttgta aattaaatcc tgcaaaagaa ggcaagaacg ctgttatacc
     3841 aacaatagaa gcatgttttc tatgacaggt cgcaaagctc tcattgacaa aaatttctgc
     3901 ttttgaggct aattcttttg aaaattcttc gctattttct tcttctcctg gattgaatct
     3961 taaattctca agaatttcat aagctccttc tcctaaattt tcattaaaat agggaactaa
     4021 ttgctgggta cttaaattta gatctacttt tccatctggt tttcccatat ggcctgcaat
     4081 tactggtata ccgccttttt caatgatata ttttagtgta ggtagggaag agtctagtct
     4141 aaaagcttct tttacaaccc cattttctac tgggacgtcc agatcacatc tcacaaatac
     4201 tctagtacct ttgtttatag gggcatcgat tagtaatttc atatgattat tttagcatta
     4261 aataaagact gtgacgggtt gggttacagc atcattaaag ccatctcaac gagtctgcaa
     4321 ctgtatcccc attcattgtc ataccaagca aaaaccttta ttagatttcc atctatgact
     4381 ttagtcatag atagatccac aatagcggaa taaggatctc caataatgtc tgacgatact
     4441 agcggttcaa acgttgctct tacaaccccc ttgtattttt cttcttttac nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn tctgcaacat tttgattatt tgtgatggcg
     4621 tgaatagtgc tcattcccgc tttcaatatt ttaaaattgg aatcaagaat tgctaagaca
     4681 ggggagatgc agtttgtggt gcaggaagca tttgagatag cgttttgtcc caagtattgt
     4741 gaatcattga ctcctttcat aaaggtttga atagttccgc ctttaaccgg agccgatatg
     4801 attacatttt tagcaccagc tttgatgtga gctagcgaag tatcgtcttt aacgaacctt
     4861 ccggtacatt caagaacaac atcaacatct aaatctttcc aaggaagttt ttcaggttct
     4921 ttttctgctg tgactaggga tttttttcct tcaaccacga tatagttttc ggttcctttg
     4981 gtatcaaaaa aacttttttc accagtgttg tcttctaaat taattctttt tccatctatc
     5041 tcaacccaaa tttctttatc ataaacaccg taaatagtgt cgtgttttaa taaatgggct
     5101 aagaccagtg ggtttgtaag gtcgtttatt gcaacaatat ccagaccttt atcaaaagca
     5161 attttaaagg aggatcttcc tatccttcca aaaccgttta tagctattcg ggcagtttta
     5221 ttttccatat actttaataa tactacttta aatgcgaatc aatccagctt tttagcattt
     5281 ctttaggcat tgcgcctact tttctgtcta tttctttatc atcttgtaca aggacaaatg
     5341 taggtatact cattactcca aaatttgcag cggtactgct gtctgactct acgtcaattt
     5401 tttgaaattc aaccttgcct gcgtattcag gtttcatctc ttcaaataca ggtgccatca
     5461 ttttgcacgg accgcaccaa tctgcataaa aatctaataa tttaatcatt tttattctcc
     5521 taagattaaa attttattaa gtcaaaaatc ttacgctata tgataacata acttttgttt
     5581 attaattatg caagacttgc ctgataaaaa gttaaaactt cttccggttt ctttaattat
     5641 tgcactggtg tttggtgttg gagtttttgc ttttagaaag ctttttacaa gagagatcgt
     5701 tataaaagag gatttgtctg aaaaaaagta ttacacaagc ttgttgagga gtgaaaactt
     5761 ggggagcgag tgggagccgg gggagatgtt ggaggttaac ctggaagatc agcctgaaat
     5821 ttctgcggag agcggtttgg tagttgatat taattcggga aagattgttt ttgaaaagaa
     5881 ttccaaggag aaaatgaaaa ttgccagttt agtgaaaatt atgactgcgg tggtggcttt
     5941 agagcataaa aatttagatg aaaggattgt tgtttcgaaa aaatcagcgg gggtaggtga
     6001 aaatacgatg ggaatttctg aaggtgaggt ttatacgttg gaggagcttt tatatggatt
     6061 aatgttgaat tccggaaatg attctgctta cgcaatttca gaaggtgttg caggtaatgc
     6121 agaggatttt gttgactgga tgaatataaa agctaaggag ttaggtttaa atgatacaga
     6181 gtttttttgt cctagcggac ttagcgatga aacttacagt accgcggttg atttggttaa
     6241 gctttcaaga tatgcattgc aaaatgaaga ttttagaagg attgttggaa ctgttgattt
     6301 tgaattgcct tattcggaga ggcataaata tttgtattta gaaaatcaga cgaatttgtt
     6361 aacaacttat cccggtgtgg gtggtattaa aactggatac actgaagagg cggggtactg
     6421 cctggttact tatgcaaaaa atgaaggagt tgagttgatt ggggtggttt taaactccgt
     6481 aaacagaaaa tttgatatgt taaatctgct aaacttcggg tttggggttt acgggataga
     6541 ggttgagagt gggtatgagc tgtgaggtac tgctgatttc tgaaaatgat ataatacggt
     6601 catggagaac aatggaaaaa acaacaatat actgcctttg gtagtaattt ttttagttat
     6661 tgcaggtttg attggaggag cttattggtt tggtaagagt agtattatgc aaaattccca
     6721 aacaggtcaa atgaccgatg taaaagaatc aactgaacca gaaaacaaat ccttacagga
     6781 acctgaaaaa tcgactacag agccttctga aaatgttgaa ttagagggag agacaggggc
     6841 gggagaaaca gaagacaagc ctacagaaga aggacccgcg gttgaagagg attccgacga
     6901 atcagtaatc gaatctctaa aaattctttt tgcgcaaaaa tatgaaaaaa gaatcgaaga
     6961 cattacagtt accatctcca aaagagaagg aaactatctt accgggggtg ttaaattcac
     7021 tgctggtgga gaaggtggtt acgttttagc agcaaaagtt aacggagctt ggaaaattgt
     7081 ttttgatgga aacggaagtt ggacttgcag tgccgtagat gttgttgatt ttccttctac
     7141 acttgcaccc gaatgctggg atgaagacac catggccgtt gttgatagaa cagctaatta
     7201 gtttatccta gttttttatt agatattctt gattttatag ttgcaaatgc tcgcagtgtt
     7261 tgtattaata aaaaaggcct agtcttttgt tataaaaact aagccttcat ctgcagtatg
     7321 ttgggtaagt atccccaaac caaagtaaga aacatactta cctgtatttc cacccaaaac
     7381 tttccctaca attatcaaga gtacgccctg tatcgagtgg tggaagtgat atttcccaaa
     7441 atgggtggct ctccttccag tttttggaaa acagttataa gcagcaactt ctagctttcc
     7501 aaaaataaaa ccagtgaaga aacaaattaa atttttcaca ctacctcctt tttctaatat
     7561 gtttatcgtc tatcttacta gaaatgtttc tattatcaag tattatcaat ttttcccact
     7621 attttttaca cttaatttca tacttgccat ctgttatcat aactccgtgc caacaaactc
     7681 tgaattattt acgggattaa atccccaaca aattgatgct gttaagcatt tagacggccc
     7741 cgcactcgtt gtggctggac caggaagtgg taaaacccgt gttttaactc accgagttgc
     7801 aaatttaatt caaaaccacg aggtttctga aacaaacatt ctctgcgtta cttttacaaa
     7861 caaagcagcc gatgaaatta aacaaagagt aggggcaatt ctttctgatt catcaggaat
     7921 atcaaaatta ccatggagcg gaacatttca ttcaatttgc tcaaagattt taagaaaaga
     7981 tggatataac attggtatac cgccatcatt tgttatttat gacaccgaag attcagcctc
     8041 cgttattaaa attatcttaa aagattttgg aattgatgca aaaagatttc atccaagagg
     8101 tgttttggaa accatctcaa gtgcaaaatc agaattaatc accccatctg attacgagag
     8161 ttacgctcaa ggttactacc agcgtactgt atcaaaaatt tacccggaat atcaaaaaag
     8221 gttacaaaat agcaacgctc tggacttcga tgatctgcta tattttactg taaagttgct
     8281 cgatacagat aaaaaaacat taaaaaaata tcaagagcag ttcaaataca tattggtcga
     8341 cgaatatcag gacacaaaca aagcccaata tgttttaacc cgtcaattat cagatttgca
     8401 tcaaaatctc tttgtggtcg gggatatgtc tcaggccatt tatagctttc gtggtgctga
     8461 ttttcgtaat attttaaatt ttcaaaaaga ctatcccgag gcctctatct acaatttaga
     8521 gcaaaattac cggtccacgc aggttatctt agatgccgca aaaaacatga ttaaaaaaaa
     8581 ttctactcat attcctttgg atctttggac ggacaaagag ggtggtgaca agatagtagt
     8641 cttttcggga agcaccggaa acgaggaaac tgcctttgta attgatgaaa tttcaaacca
     8701 gatgaagtta ggaaagacat ttgtggatat ggctgtttta taccgtacta acgcccaatc
     8761 ccgagctatt gaggaggctt taatcaaaaa cagtattccc tatagagtgt ttggtggggt
     8821 gcgtttctat tccagaaaag aaataaaaga catcgtctca tatttaagag tgattcagaa
     8881 tccaaaagat tcggtctcgt ggtcaagaat tatcaacgtt ccaactcgtg gcatgggcca
     8941 aaagtcagct gataaattaa aaaatgcaga ttgggacctt gatgaaatcg aaaaagtttc
     9001 aaaaattcca ataaaaagat ggatttcaaa aaaagatgac atgtctacat tagaattaat
     9061 ggaagaggtt ctaaaagata ccggttatat taaatggctt gatgatggca cagaagaagc
     9121 tttaagtaga attgaaaaca ttaaagagtt aaaaacagtc gcctcacagt ttgtaaattt
     9181 aagtgagttt ttagaaaatg tttctttgat tgaagatacg ggaaaagcca gtgaaaaaaa
     9241 caatgttgat tgtgtgactt tattaacagc gcatgcttca aaaggtttag agtttccaat
     9301 tgtttttatt atcggaatgg aagaaggttt atttccccac tcaaattctt tgggtgagca
     9361 agaagagctt gaagaggaga gaaggctttg ctacgttgct ataactagag caaaagagaa
     9421 ggtgtatatg acaaacgctc gttctaggat gtattttgga aatattcagg ctaatcttcc
     9481 tagtaggttt ttatcggaaa tcccacagga gttattggat tttagaggat taggaataaa
     9541 aaattatttt cccaggagca acaaaaacga cgattttctt gatgacctcg aattcaatag
     9601 aggtaatttc aaatgggact agttttttaa tgtaaaatta atgtatgttt aaacctcaca
     9661 aagagcttgg gcagaatttt cttacggata aaagtgtggc taaagatatg gtggaagctc
     9721 ttgatattca aaatggcgac gaaatagttg aaattggtcc cggccacggt gttgttacag
     9781 aatttttagt cgaaaaaata aagtatttga atgtaaaaat ccatgcagta gagttggatg
     9841 taagacttta ccaggaactt aaaaattttt attcagggaa tgaaaagata gagataataa
     9901 aagaaaatat tttggattgg cttccaacat tctcaacgga cagagaaata aaaataatag
     9961 ggtctgttcc ttactacata acatctccaa taattcataa aataataaaa atgcctgcgg
    10021 ttcctcaagt ggtagttttg ttagtacaaa aagaagtagc tgaaaagata aaaagtaagg
    10081 caccggattc ttcttacatg tcctcgtttg ttcaaacttt ttttgatgtt aagtatttag
    10141 gaaaagttcc cagtaaaaaa tttaatcctg aacccaaggt tgatggcgga attattaaaa
    10201 tggtcaagaa agaagatgca aatctcgacc ccgtttttat taggaaatac gagggttttt
    10261 tacacaaggc ttactccaat ccaagaaaaa tgctaaacaa agtattcagg aaagaggagc
    10321 ttgagaatgg taatattaat ccaacactcc gtgcgcaaaa tttagatgct gaagagtggt
    10381 tgagttttta taaaaaaata aatgaaattt agtatttgca ttccaacttt aaacgaggaa
    10441 aagtacatag gcgggatatt aaattgtcta gaaaaacaga catttaagga ttttgaagtt
    10501 actgttgtag acggaaattc acaggacaga acaaaagaag aggttttgaa gttttctagt
    10561 aagtatccac ttcaattttt gaaatcacct caaaggggag tgtcgtttca gagaaattac
    10621 gcagaaaaaa agtcttccgg agattatgta atattttttg atgctgatgt aatgatagag
    10681 agcgattttt tggaaaaaat aagcaaccat ttagaaaaag gtgatattga tgttttaact
    10741 acttggaata agccaatatc cgataaacca atagatgaat ttatctactt atttgtaaat
    10801 atttttatgc tggagcttat taaaaagaaa aatcccggag ctgttggcgt ttttatatgt
    10861 gttaaaaaaa gctcgtttga aaaaatcgga ggttttagag aaaaagttaa ttttggagag
    10921 gactttgatc ttgcaaagag gcttcatgat agtggtttta catacgcttt attgaaaaaa
    10981 ccaaaagtac acgtatccgt gagacgtttt gataaagagg gaagattgaa catgataata
    11041 aaaaatctta gagcggctac atattatcta acgaacaaca acaactacat cgacaagatt
    11101 cagggtaagt ttaagcacga gttcggtaaa ttttaattgg aaatttttta aataacctta
    11161 aagtctgttt tcctaaattc tctaaaatgt agattagatc gaagcccgcc aaatttttaa
    11221 atttttgaac gggctttttt ttcggcccga ttttcatcaa agcctgtact ctttgatccc
    11281 gcccttatag gacgatatta aattgtattc ataaaatatc ctatattggc cttggagtca
    11341 atgatatact gggtttatga aaagacacat tcttcaatcc aagctttggg agaagtttaa
    11401 aaacgactat ggaactccgg cagttcgggt tggaaaaatt ctttatacaa aacataaaat
    11461 tccttttact aacaattatt atggatattg ccccagagtt aacccttttg atattgattt
    11521 tgaagaatta aaaaaatcgt taaatgaaaa taattgtgta gctgttcatt ttgatgtccc
    11581 caatgttact aaagacgacc ccgaagctga aaaagcagag caaatactta aagagaaatg
    11641 tgttttatct cagagagatg aatttgccaa aggaaatttt ttaatgagtc ttaaaaaaag
    11701 cgaagaggaa cttttagcca atatgcataa aaaacaccga tacaacatag gattggctca
    11761 aagaaaaggc gttacggtga gagaaactac tgctgacgaa gattttcaaa ttttttatga
    11821 tctttatttt gagactaccc aaagacagaa attttttggt aggtccaaaa catatttgaa
    11881 cggtgtttgg aagacattta aaagtgcaaa ttccgcacat ttgctgattg ccgaatatga
    11941 agggaagccc ttgtcctctt ggatgttgtt ggttcaagat ggagtgcttt actatcctta
    12001 cggtggttca actgaaaaag ataaagatgt gcaatcaaac tgtcttttgg ggtgggaagc
    12061 tattaaattt ggaaaaaagc tggggtgtga tttgtttgat atgtggggtg ctgccgaaga
    12121 tatgagtaat aaaagtgacc cttactacgg attttcttta ttcaaagaga agtttggagc
    12181 aacccatgtt agttatatta aatcttatga ttatgttata aacgaaccct tatacaaaat
    12241 gtttttggtg gcaaataatt tgagatggaa gtttttgagt atactaaaat gaagcccgaa
    12301 ttggatattc gacagagtga taaatgggca aaatatcttg gaagaatggg gtgggaaagt
    12361 catcgtacat cgagtggtat taatatttat tttcatagaa cttttcttgg aacaattatt
    12421 aaaattcaaa aacctctgtc ggttagtaaa aaagatatta aagaaattga ggatttttgt
    12481 gtaaaattaa agcctctttt tattaaaata gagcctttta taggtcagga gattgcggtt
    12541 ttggaggagt gcggttatgt aaaaagtgag gtggcattaa cacccccctc aactatttat
    12601 attgatctta caaaatcaga aaacgagctt tgggaaagca tttctcacag tggaaaatat
    12661 tcaataaaaa gagcccaaag agaaggtact gtgacgagat tctatcaaaa tcctattaaa
    12721 gataagatgg gtgcctattt tgagatggtc caagttacag gaaaaagaaa acatttctat
    12781 gttcaaccaa tggttgattt cgaagaaaaa gtgaagatat ttggaaaaga atgccacctc
    12841 gttttgtcct atgataccga aggaaaacta ctaagcggaa aattttattt atgccatagg
    12901 gatatggttt tatactcgac cggtggcacc tctgaaaagg gattaaaaac caaggccggg
    12961 tacgagctcc tgtggaaatc tattttatat tttaaaggtc ttggatataa ggttttggat
    13021 cttgagggta gggatgaccc acgttttaaa gatgctacca aaaattgggg aggtttcagt
    13081 cattttaaag aaaaattcgg tggggaaaat attgagtttc ctcacccata cattaaatac
    13141 tttaatcctg tattgaagtt tttttccaag tttatgaaat taccgctgta gagggttgag
    13201 tcctctccct tactccttct ttgcgaaggg gcatatctct ttaaagtcgc agtacataca
    13261 atcgcgtccg ggattaggtt caaaattttc ctcatttatg tttttaatca cgtctttgat
    13321 gatttccctt tgagcgtcaa cctgtttctg agttcttttt gtggaaattt tattgcccgt
    13381 atttaaaaaa tagagactca agacatccgg atttattttc aaagcctctg ttgcccccat
    13441 agtgtaaata gtcatctgta cgttgtcatc aacatctttt tgggattttt ccttccctgt
    13501 tttgtagtca attatttcta cggtgccatc aggaagttta tctatcctgt ctattgttcc
    13561 tttcagctta gttccatcaa tactaagtac aaattttttc tctaagccca agtgtttagc
    13621 atctaaaccc ttgttttttt cataataatc cttaagaatg tttttacctt cttcaaaaga
    13681 attttttcta tctttttcat tttcataacc caacggattc cagttttttt caaatatttt
    13741 ataaaaatct tcgagcgaag gtttgtttcc agacattatt tttgagtgaa attca
//
LOCUS       DAOTFA010000006        13620 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate AMR_MDS_3115
            NODE_3128_length_13620_cov_7.084924, whole genome shotgun sequence.
ACCESSION   DAOTFA010000006 DAOTFA010000000
VERSION     DAOTFA010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868139
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (wastewater metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 13620)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13620)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:36:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 536
            CDSs (total)                      :: 521
            Genes (coding)                    :: 520
            CDSs (with protein)               :: 520
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13620
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3128_length_13620_cov_7.084924"
                     /isolate="AMR_MDS_3115"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..6863)
                     /locus_tag="P5014_00520"
     CDS             complement(<1..6863)
                     /locus_tag="P5014_00520"
                     /inference="COORDINATES: protein motif:HMM:NF017930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HRY56629.1"
                     /translation="MENKVNFYTLGRDIAIKKKFPSKRLFSKFNFDRQLDYKSLIENI
                     DSLYSYLVIEFEKNPEIVPLSSSWILDNYYLINQQSSYFSETFKPKILNKLPLSEYSK
                     IYFQIHYIARKYLEASIYEFSEETLIPFLKGYQKIYPLSSLELWVLPTIIKFEIIKSI
                     LDKGLEIKEKIENSKLAASIVSDISSDIDKGKNPAKRIKKIIKDVSVSPHVLLLVLED
                     IRELDYRVEEALKLVEGKIHEEDLNLEEFTRASVLRDLDLKQGLNNAVTSIRNFSYIN
                     WSKFFTKVSIVENTLSIDPSGIYPLMDDISRDRYRHIIEDMSSNSNFSESEIARKLVE
                     QSSEANIDVQRHVGYYLIDDGYYSFARKIGYKPPLKGALKILVKKYPDLSYFGLNVSL
                     VILLSLFSFSFFFKNTQNLLLTIILTFLQIVPSSNLCIFFTNKFFTWLMPPALPPKLE
                     FENHIPEEYKTLVVVPSIFSGKGKVDILVENLEKRFLTNKFNNLYFALLSDFKDSPYE
                     ETDQDSKDLTYLRKKIKQLNMKYKSSGFDVFYLFHRKRLFNPREGCWMGWERKRGKLM
                     QLVEWLSEKNKSENKSFSVVDGDVSRLYGVRYVITLDEDTSIPADVSLKLIGAMAHPL
                     NFPILDEDKKIIKRGYGFLQPRIIIKPETEHNSPFTRIFVTDSGFDSYSTAISDIYQD
                     VFNRSNFMGKGIFDVRAVKETLLGKFPENALLSHDLIESCFAKSGFLSDTSLVEDFPT
                     SYKSYVNRDNRWVRGDWQLLPWLCPMIPLANGTKAKNPLDMMSLWMIIDNLRRSLVVP
                     IFIILLYINWFFSSSEAINIYILVLFFGHLLFEFFSIKRYWVHKLPISEKIRLTLSFM
                     LQKIILTSSFFIMASVTAINKILSIFRTLKRLITHRNLLEWMTHSEAEHVKSENNILG
                     YFLTSKAVYLNLVILFSLFVLGKLTVLNIFTLFIWILSPIFVFVISKPYKQIQISDRL
                     KDKDKMLSYSKMIWSFFEQHVNEESNHLPPDNIQLTPSKKITYRTSPTNIGFYILSLI
                     SAYDLKIIDLETYVSRLWNTLNIVYRLKKYRGHLFNWYDLKKLEPLNPYISTADSGNF
                     VAALMLAEEACSSMSSALKKSNLEISKKFLQLSKLSRRIYQKTDFTFLYDKNMSVFSI
                     GYNVSARQRDSSYYDILASEARIASYVAIALDQVEVKHWFNLSRPVRIRKGKFYLMSW
                     GGTMFEYLLPALLLNEKKNTFLSQTTEQIFYIQKKYAEENGIPWGISESNFDAFDFDS
                     NYQYKMMGVPEIALKRYDSRDLVVSPYSSFLSLMIAPEESERNLHRLEDDGVLGPYGF
                     YEAVDFNRSTNEPKVGENVKIYTAHHQGMSLVAINNALNNRIMVGRLHSNDYIKSCDI
                     LLDEKIPQVSNIVEANQYGFDRKLSTVRRYEKERVRYSNTPNTLFPIAQLYGNGKYSV
                     MISNSGAGFSKFRDTFINRYNEDLTLDDSGNHIFIKDVENSYFWSATYQPTLVKPDNY
                     EVRFYTEYAEFSRLDKKTFTNLNIFVPPEKNFEIRELTLTNQSDNSKEYEITTYSEIM
                     LDDLKAGLSHPVFNKLFIETAYEDGVLIAKRKKRYIRDIEKYAFHFGFSEDVNLKTDS
                     YETSRQNFIGRIKHIQNPAAMVSELTNSIGTVLDPIFSIRYKISLKPGESKKIHFIYG
                     YANNIHEIKAARELLFTGEQMNELRYLNTHYIEAKRTHLGIDVRQEILFQKLFSRLVY
                     PDYRLKETQSIDHTAGRSLLYRFSISGENRILAVKVNSKNIELVRNLVLLHEYLRMLR
                     FNFDLVFIVDEKISYEETLEQDVYEVVENSLSHPYKDSGVYVLNVINMTLEERYTILS
                     LAKVVLDSSLGSLDDQINLASKYSEPESVGIKDVVYEDYVESIVPAVDFKEGLEFYNG
                     IGGFSKDGENYVMDISAENPTPLPWSNVIANERFGTMVTESGLGYTWNINSQMNKISP
                     WSNDFVSDIQGEIIYLKDADSEKVISATPLPNKSGNFRVTHSQGYTEYKGESFDVEHT
                     LKVLVDINDPVKILKLTLKNNSKYTKTMEATYYVEFTLGEDRYTNQENLKIEFDPVRQ
                     SILAQNPYSNNFSNQTAFMATNRPVVAATSDRKKFFGMGNSKKVPLWIYKNVRGITGF
                     KSVSDKCGVITTEVEIPKNSKVSLLFILGQDDNVDNVNRLIDKYTNLEAVEQSVKDIQ
                     EKWNQVKSKIKINTPDTKFNYLINNWMLYQVLSCRVDGKTAFYQAAGAFGFRDQLQDV
                     MSLVYCNPSKVRDHLTLCCAHQFKEGDVQHWWHYPSGAGV"
     gene            6975..7361
                     /locus_tag="P5014_00525"
     CDS             6975..7361
                     /locus_tag="P5014_00525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56630.1"
                     /translation="MKKIFNFLIIVGIAYGIYYFADQTRIFKRLNCLGLGGTPVNVRE
                     VGANIISNDIVCAEPTTDANKECFDNTDCNEDCILERYSDEYPDVTTFLDKHGYDKRG
                     YCQPYEDMDCYVSRDRGTIVIHKCSK"
     gene            7361..7768
                     /locus_tag="P5014_00530"
     CDS             7361..7768
                     /locus_tag="P5014_00530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56631.1"
                     /translation="MNQKIANYKKFIEANIIEAKNEEDLDLLLKLNSSAISNFQAERL
                     IHLIVTMSFALFTLVFIYFYLFFDVTIFGIVCLITIVVLIFYILHYYKLENGVQSLLE
                     YDQKILNRKKELDQQKLPTNQVVTQTPQFRTGL"
     gene            7808..8299
                     /locus_tag="P5014_00535"
     CDS             7808..8299
                     /locus_tag="P5014_00535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56632.1"
                     /translation="MNHFYLSTFQPSDKGNVPIVLTKLIPDIKIDINSDNFIFYTTGF
                     NNIKVLENDILRNTFVVIKKFDKLTGNYFKPIFQWSGRHNFEDILLAIKSLGYKSFRL
                     ILNDDFRIVSGHRRAVKALERQISSQTGLRIDRVNPSTEIWILHTKDGYGFVMLKISN
                     VKK"
     gene            8359..9066
                     /locus_tag="P5014_00540"
     CDS             8359..9066
                     /locus_tag="P5014_00540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014679388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HRY56633.1"
                     /translation="MKNILNKLKTELTSKTNQSGIKTSQRFFKDPINTYGMKSKDIVI
                     ISQKYFQLIKNLSKEEMCKLCEVLFKSDMYEEFMIAANWIQKRDKEFISKDIDLYENW
                     INKYVNNWAKCDVFCNHSVGSLVERYPKLIKYPKKWAKSKNLWMRRASAVSLIIPAKN
                     GLFLNDIFEICDILLEDREDMVQKGYGWLLKVTSQINLKMVFDYVMKNKGKMPRTSLR
                     YAIEKMPKDLKEKAMKK"
     gene            9100..9798
                     /locus_tag="P5014_00545"
     CDS             9100..9798
                     /locus_tag="P5014_00545"
                     /inference="COORDINATES: protein motif:HMM:NF012449.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0030955 - potassium ion binding [Evidence
                     IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HRY56634.1"
                     /translation="MRTNVIATIGPSSIDKDILGQLISEGVDIIRINTSYGDIEQYKK
                     VLQNLKEVDPNSRVKKMIDIKSEDILNFCDENKMDFIAVSFAESKEQIENIKNKHPDC
                     VLISKIESVKGVTNYHDILEVSDGVMIARGDLSSAVTLEKVPPLQKQFTKEAILKNKY
                     VIAATEMLLSMTDKPYPTNAEVSDVANAVFDGVNAVMLSEETAVGKYPVESVKIMEKI
                     ITEAENYTREQNLI"
     gene            complement(9804..12326)
                     /locus_tag="P5014_00550"
     CDS             complement(9804..12326)
                     /locus_tag="P5014_00550"
                     /inference="COORDINATES: protein motif:HMM:NF012905.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HRY56635.1"
                     /translation="MKFSEYTTKTNQTLCEEFNTDIEKGLSFQKANIRKGRYGPNTLR
                     ANESTWFDILLRQFKSAFIYLLIAASVISFLLNNRFDSLMILLFVVINTGLGFYQEFK
                     SQKALSYLKNLVDRQVRVIRSDQVRYISSTDLVPGDVVLLEVGDIVPADIRLVMEDNF
                     YVDESSLTGESVPVKKISTPLKEKETQIYKASNIVFMGTTVSEGNSKGIVIFTGKNTE
                     IGKITKLTVETSDTSGFEKHINKFSKFILILTSVTLVVVFLAHLFIKGQDIAIPELIL
                     FSIALAVGVIPEALPLVTTFSLTQGALKLAKKKVVAKRLSAIEDLGSIEILCSDKTGT
                     LTENSLSVSEIFSEDKDKTLLYSALAGDVFGKKKTANNSFDLALFKKIGRFELTKLKN
                     VKRLNENPFNPVRRRNSVLVEIESKRYIIVRGAQESILPYVSNISNEDEKNILEWILD
                     QGKKGRRVITVARKEFSKDDYDVEDEESNLELVGLISFSDPVKISSRSAVNKAQELGV
                     RIKIITGDSAEVAGAVAYEVGIAKSSSEVISGFDLEKMNYEEKISAVEKGNVFARVSP
                     EQKFQIIEILQKKYTVGYLGDGINDAPALKLANVAIVVNGASDVAREASDLILLQQDL
                     KVIIDGIEEGRKTFVNTSKYIKATLSSNFGNFYAVAIASLLIEYLPMLPVQILLVNLL
                     SDFPMISIATDNVEKEEIKDPCVYNNTEIVIMATFLGIVSTVFDLLLFAIFKSSKPEV
                     LRTAWFIESILTELILIFSIRTKKFILKSRSKPSRIIIFLTSLAAVSTFVVTFLPFGK
                     NTFGFTPLKYSNLLTIIVIAAGYFITSEFVKLSIYRNKVKNV"
     gene            complement(12402..13442)
                     /gene="trpS"
                     /locus_tag="P5014_00555"
     CDS             complement(12402..13442)
                     /gene="trpS"
                     /locus_tag="P5014_00555"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HRY56636.1"
                     /translation="MISEPKNTLSNLMTGIRPTGPLHLGHYVGALKNWVELQDKYECF
                     FMIADIQALTTHADNPKLIEESVREVVLDFLSIGLNPRKENVHFILQSAMPELTELTV
                     YLSMVTPFTWMQSNPTIQTESKYLGKNISTGFMYYPVSQAADILFSSTDPKKSKEPIL
                     VPVGEDQVPHLRDTNNIVSAFNRTYSPTFVECDALVGKIGRLVGTDGQAKMSKSLNNA
                     IFLKDDENTVKKMIKTMYTDPKRIHPNDPGTVEGNPVFIYHDVFNINKEEVEDLKNRY
                     KKGTVGDVEVKEKLEKSIQEFLEPIRESRKKHEKEDIKGILDEGTVFARNLAQKTLER
                     TKEAMHLNYSSL"
     gene            complement(13539..13612)
                     /locus_tag="P5014_00560"
     tRNA            complement(13539..13612)
                     /locus_tag="P5014_00560"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13578..13580),aa:Trp,seq:cca)
BASE COUNT         4553 a         2317 c         2085 g         4665 t
ORIGIN      
        1 acccccgccc ccgaaggata gtgccaccaa tgttggacat ccccttcttt aaattgatgc
       61 gcacaacaca atgttaggtg atctctaact ttactgggat tacaataaac caatgacatc
      121 acatcctgaa gctggtctct aaatccgaaa gcacctgctg cttgataaaa ggctgttttt
      181 ccgtcaactc tgcaagataa tacttgatat aacatccaat tatttatcaa gtaattaaat
      241 tttgtatctg gtgtattaat tttgatctta gactttactt gattccattt ttcctgaatg
      301 tctttaacac tttgttcaac tgcctctaga ttagtatatt tatctattaa tctattaacg
      361 ttgtctacgt tatcatcctg acctaatata aataacaggc ttactttaga atttttagga
      421 atttctactt ccgtagtaat aacaccacat ttatcagaaa ctgatttaaa tcctgtaatt
      481 cctctcacat ttttataaat ccataaagga acctttttag aattacccat tccaaagaat
      541 ttttttctat cagaagttgc tgccacaaca ggtctgtttg tagccataaa agctgtttga
      601 ttcgaaaaat tattgctata aggattttgg gctaggattg actgtctaac agggtcaaat
      661 tcaattttta aattctcttg attagtatac ctgtcttctc ccaatgtaaa ttccacataa
      721 tatgtggctt ccattgtttt tgtgtattta ctattgtttt ttagtgttaa tttaagaatc
      781 ttaaccgggt cattaatatc aactaaaact tttaaggtgt gttctacgtc aaaactttca
      841 cctttgtatt cggtataacc ttgggaatga gttaccctaa aattaccact tttgttgggg
      901 agcggggtag cgctaattac cttttctgaa tcagcatctt ttagatatat tatttcgccc
      961 tgaatatcac ttacaaaatc attagaccac ggggatatct tattcatctg actattaata
     1021 ttccacgtgt aacctaaacc cgattctgtt accatagtac cgaatctttc gttagcaatt
     1081 acattagacc acgggagtgg ggtagggttc tctgctgaaa tatccataac atagttctca
     1141 ccatcttttg aaaatccgcc tatgccgtta tagaattcta aaccttcttt aaaatctact
     1201 gcgggtacta tactttcaac atagtcctcg taaacaacat ccttaatacc tacggattca
     1261 ggttcagaat atttagacgc aaggtttatc tggtcatcaa gtgatcctaa agaagaatct
     1321 aatacaacct tagccaatga cagtatcgtg tacctttctt ctaaagtcat attaataaca
     1381 ttgagaacat atacacctga atctttgtat gggtgagata acgaattttc tacaacctca
     1441 taaacatcct gctccaatgt ttcctcgtag ctaatcttct cgtctactat aaaaaccagg
     1501 tcaaagttaa atctcaacat tctcaaatat tcatgtaata gaactaagtt tctaactagc
     1561 tcaatatttt tactatttac cttaacggca agtatcctat tttcacctga tattgaaaat
     1621 ctatataaaa gacttctacc ggcagtatga tcaatacttt gagtctcttt taatctgtaa
     1681 tccggataaa ccaatcttga aaatagtttt tgaaatagta tttcctgacg tacatctatt
     1741 cctaaatgag ttctttttgc ttctatataa tgagtgttaa gataacggag ctcgttcatc
     1801 tgctcaccgg taaagagaag ttctcttgcg gcttttatct cgtggatatt gtttgcgtaa
     1861 ccgtatataa agtgtatttt tttggattca ccaggtttta atgaaatctt gtatcttata
     1921 ctaaaaatgg ggtccagaac tgttccaata ctatttgtta attcggagac catagctgca
     1981 gggttttgaa tgtgtttaat cctccctata aaattttgcc tgcttgtttc ataagaatct
     2041 gtctttaaat ttacatcttc agaaaaacca aaatgaaaag catatttttc aatatctcta
     2101 atgtatcgtt tctttctttt agcaattagt acaccgtctt cgtaagcagt ttcaataaaa
     2161 agtttattaa aaacaggatg tgatagacct gcttttaaat catcaagcat tatctcgctg
     2221 tatgtagtaa tttcatactc tttactgtta tccgattgat tagtaagtgt aagctctctt
     2281 atctcaaagt ttttttcagg tggtacaaat atatttaaat tagtgaaagt cttcttatct
     2341 aacctactaa attcagcata ttctgtgtag aacctaacct cataattatc cggttttact
     2401 aaggttggtt ggtatgtagc agaccaaaaa taactatttt ctacatcctt tataaaaata
     2461 tggttccccg aatcatctaa agtaagatct tcattatatc tattaataaa cgtatctcgg
     2521 aatttgctga acccagctcc ggaatttgaa atcattacgg aatattttcc attaccgtat
     2581 agttgggcta ttgggaataa ggtatttgga gtatttgagt acctcactct ttctttttca
     2641 taccttctaa cagtacttaa ttttctatca aatccgtatt gattagcttc aactatattt
     2701 gatacttgag gtattttttc atctaataaa atgtcacaag actttatata atcgttgcta
     2761 tgcagtctgc ccaccattat tctattatta agtgcgttat tgattgctac caaactcata
     2821 ccttggtggt gagcagtata aatttttaca ttttctccaa ctttcggttc gtttgtagat
     2881 ctgttaaaat caacagcttc gtaaaaaccg taagggccta aaacaccatc atcttccaat
     2941 ctatgcaggt ttctctctga ttcttcggga gcaatcatta atgataggaa tgatgaataa
     3001 ggagatacta ccaaatctct tgagtcatat cttttaagtg ctatctcagg aactcccatc
     3061 atcttatatt gatagttaga atcaaagtcg aatgcatcaa aattagattc tgagattccc
     3121 cacggaatac cgttttcttc cgcatatttt ttttgaatat aaaatatttg ctcggtagtt
     3181 tgactcaaga aagtattttt cttttcattt aacaagagcg ccggtagaag atattcaaac
     3241 atagtcccac cccatgacat aaggtaaaac tttcctttcc tgattcttac cggacgtgat
     3301 agattgaacc aatgcttaac ctcaacttga tctaaagcta tagccacata actagctatt
     3361 cttgcctccg acgctaagat atcgtagtaa ctagaatctc tctgtcttgc agagacgtta
     3421 taaccaatag aaaacaccga catgttttta tcataaagaa atgtaaaatc agttttttga
     3481 taaattcttc ttgaaagttt tgatagttgg agaaacttct tgctaatttc aaggttagac
     3541 tttttaagtg cactagacat tgacgaacaa gcttcctcag ctaacatcag agcagcaaca
     3601 aaattaccac tatctgctgt ggatatatat gggttaagtg gttccaattt tttcaagtca
     3661 taccagttaa acaaatgtcc tctatatttt tttaatctat aaacaatatt aagagtgttc
     3721 cataatcttg aaacgtaagt ttctaaatct attattttca agtcatatgc agagatcagg
     3781 gatagtatgt aaaaaccaat atttgtcggg gacgttctat aagtaatttt ttttgaaggt
     3841 gttagttgta tattgtcagg cgggagatga ttactttctt catttacatg ctgttcaaaa
     3901 aatgaccaaa tcatttttga ataactaagc attttatcct tatctttgag cctgtctgaa
     3961 atttgtattt gtttatacgg tttactaatt acaaaaacaa atataggaga caagatccaa
     4021 ataaataagg taaaaatatt taaaacagtt agttttccca aaacaaataa agaaaacaga
     4081 ataactagat ttaaatacac cgcctttgaa gtcaggaagt atccaaggat attattttca
     4141 cttttaacat gctctgcctc actgtgtgtc atccattcaa gtagatttct atgggtaatg
     4201 agccttttta aagttctaaa aattgagagt attttattta tagcagtaac agatgccata
     4261 ataaaaaatg aggaagtaag tataattttc tgcaacataa aagagagagt taaacgtatt
     4321 ttttctgaaa taggaagttt atgtacccaa tatctcttta tagaaaaaaa ttcaaacaat
     4381 agatgtccaa aaaataagac aaggatatag atattaattg cttcagaaga tgaaaaaaac
     4441 cagtttatat aaagtaaaat aataaatatc ggtactacca aacttcttct aagattatct
     4501 ataatcatcc acaaagacat catatccaag ggattttttg ctttcgtacc gttagcaaga
     4561 ggtatcatag ggcataacca cggtaataat tgccagtcac ctcttaccca tctgttatct
     4621 ctattaacat agcttttata tgaagtagga aaatcttcta ctaagctggt atccgacaaa
     4681 aatccggatt ttgcaaagca actttctatc aagtcgtggc ttaaaagagc attttccggg
     4741 aattttccta acaaagtctc ttttacagcc cttacatcaa atatgccctt acccataaaa
     4801 tttgatctat taaaaacatc ctgataaata tcagatatag ctgtggagta tgagtcaaaa
     4861 ccgctatctg ttacaaatat tcttgtaaaa ggagagttat gttctgtttc aggttttatg
     4921 ataatcctag gctgtaaaaa cccataacct ctcttaatta tttttttatc ttcatctaaa
     4981 atcggaaaat ttaatgggtg cgccatcgca cctatcaatt ttaacgaaac atcagcaggt
     5041 attgaggtat cttcgtccaa tgtaataacg taccttacgc catacaatct tgagacatcg
     5101 ccgtctacaa cggagaatga tttattttcc gacttgtttt tttcagataa ccattctacc
     5161 agctgcatta attttcctct tttcctctcc caacccatcc agcaaccttc tcttggattg
     5221 aaaagtcttt ttctatggaa aagataaaat acatcaaaac cggaagactt gtatttcata
     5281 tttaactgtt ttatcttttt tcttagataa gttaggtctt tactatcttg atcggtttct
     5341 tcataagggg aatctttaaa atcacttaaa agtgcaaaat aaagattatt aaacttgttc
     5401 gttaaaaatc ttttttctaa attttctact aatatatcta ctttcccttt cccggaaaaa
     5461 atagacggaa caacaaccaa tgtcttatat tcttcgggta tgtggttttc aaattctagt
     5521 tttggaggta gcgccggcgg cattaaccat gtaaaaaatt tatttgtaaa gaaaatacaa
     5581 agatttgatg aaggtacaat ctgtagaaac gttaaaataa tcgtaagtaa aaggttttgt
     5641 gtatttttaa aaaagaacga aaatgagaaa agtgaaagta aaataaccaa acttacattc
     5701 aacccaaaat acgataaatc cggatacttt tttactagta ttttaagtgc acctttcaga
     5761 ggaggtttat aacctatctt tctagcaaaa gaataataac catcatctat taaataatat
     5821 ccaacatgtc tttgaacatc tatatttgct tcggaagatt gttcaactag ttttctggca
     5881 atttctgact cactgaaatt actgtttgag ctcatatcct caataatatg tcgatatcta
     5941 tcgcggctaa tatcatccat taacgggtat ataccggaag gatcaattga taaagtgttt
     6001 tctacaatac ttacctttgt aaaaaacttg ctccaattta tgtatgaaaa attacggatc
     6061 gaggttactg cattatttaa accttgtttc aaatctaaat cccttaaaac acttgctcgt
     6121 gtaaactctt cgaggttcaa gtcctcttcg tgaatttttc cttcaacaag cttcaaggcc
     6181 tcttctacac gataatctag ttctcttatg tcttctaaga ctaataatag tacatggggg
     6241 ctaactgaaa catcttttat aattttttta attcttttgg ctggattttt acctttatct
     6301 atgtctgaag atatgtctga aacaatactt gcagctaatt tactattttc tattttttct
     6361 tttatttcta accctttatc taaaattgat tttataattt caaatttaat aattgttggt
     6421 aaaacccaaa gttccaaact tgatagaggg tatatttttt gataaccctt aagaaaaggt
     6481 attaatgttt cttcagaaaa ttcatatatt gatgcctcca aatactttct agcgatgtaa
     6541 tgaatttgaa aatatatttt actgtactct gataagggta acttatttaa tatttttggt
     6601 ttaaatgttt ctgaaaaata ggaagattgc tgattaatta agtaataatt atctagtatc
     6661 catgaagatg acaagggaac aatttcagga tttttctcaa actcaatcac tagatatgaa
     6721 tataatgaat ctatattttc tattaaactc ttgtaatcaa gctgtctatc aaaattaaat
     6781 tttgaaaata aacgtttcga aggaaacttt tttttgatag caatatccct acctagggta
     6841 taaaaattta ctttattctc catatcggtt ctccataaat attctacatt agattgaata
     6901 ggaaattaag tattttaaaa gtaaaaattc gattaactat ctaaatatta tttaatatat
     6961 tgtattatta acatatgaaa aaaatattta attttttaat catcgttgga atagcatacg
     7021 gtatctatta tttcgccgat caaacaagaa tttttaaaag attgaattgt ttaggtcttg
     7081 gaggaactcc tgtaaatgta agggaagttg gggctaacat tataagtaac gatatagtat
     7141 gcgcggaacc tactaccgac gccaataaag aatgttttga taatactgac tgtaatgaag
     7201 attgcattct agagagatac tcagacgagt atcctgatgt tactactttt ctagataaac
     7261 acgggtacga taaacgtggt tattgtcaac cttatgagga tatggattgt tacgtgtcga
     7321 gagacagggg tactatcgtt atccataagt gttcaaaata atgaatcaaa agattgcaaa
     7381 ttataaaaaa tttatagaag ctaatataat agaagcaaaa aatgaggagg acttagatct
     7441 tttactaaag ttaaacagct ccgcaatatc aaattttcaa gctgaacgat taatccattt
     7501 gatagtaact atgtcctttg ctttgtttac attagtattt atatattttt atctattttt
     7561 tgatgttact atttttggaa tagtgtgttt aattactata gttgttttga tattctatat
     7621 actgcattat tacaaactgg aaaacggtgt tcaaagtctt ttggagtatg atcaaaaaat
     7681 tttaaatcga aaaaaagaac tggatcagca aaaattaccc acgaaccaag ttgttactca
     7741 aacaccgcag tttagaacgg ggttataaaa ataaagtaat tgacgctaga tagtatataa
     7801 ttcctccatg aatcacttct atttatctac ttttcaacct tctgacaaag gcaacgttcc
     7861 gattgtttta actaaactaa ttccggatat aaaaatagat ataaactctg ataattttat
     7921 tttttatact actggtttta ataatataaa agttcttgag aatgatattt tgagaaacac
     7981 gtttgttgtt ataaaaaagt ttgataaatt aaccggaaat tattttaaac caatatttca
     8041 gtggtctgga cgtcataatt ttgaagacat tttacttgca attaaatctc ttgggtacaa
     8101 atcatttaga ttaatactaa atgatgattt tagaattgtt tccggacatc gaagagccgt
     8161 aaaagcactt gagagacaaa tatcaagcca aacgggatta agaattgata gagtaaaccc
     8221 atcaaccgaa atttggattt tacacacaaa agatgggtat ggttttgtta tgttaaaaat
     8281 ttcaaatgtc aaaaaatagt atgtgcgagg tgtttactta ttgtaaatat ttcccacttc
     8341 catctcaata tttataatat gaagaatatt ttaaacaaac ttaaaacaga gttaacctct
     8401 aaaactaatc agagtgggat aaaaacttct caaagatttt ttaaggaccc tatcaatact
     8461 tatgggatga aatctaaaga tatagtgata atatctcaaa aatatttcca attaataaag
     8521 aatctgtcaa aagaggaaat gtgtaaattg tgtgaggttc tgtttaaatc tgatatgtat
     8581 gaagagttta tgattgcagc aaattggata caaaaaaggg ataaagagtt tatatctaaa
     8641 gacatagatt tatatgaaaa ttggattaat aaatatgtta ataactgggc aaagtgcgat
     8701 gtattctgca atcactccgt cggaagtttg gtagaaagat acccaaaatt gattaaatac
     8761 cctaaaaagt gggcaaaatc gaaaaatctt tggatgagga gagcgtccgc ggtatcatta
     8821 attattccag ctaaaaatgg gctattttta aacgatatct ttgaaatctg cgatatttta
     8881 ttggaggata gggaggatat ggttcaaaaa ggttacggat ggcttttaaa agttacctca
     8941 caaataaact taaaaatggt ttttgactac gtaatgaaga acaaaggtaa aatgcctcgt
     9001 acttctctaa gatatgctat agaaaaaatg ccaaaggatc ttaaagaaaa agcaatgaaa
     9061 aaatagtaat aagataggta tttctgatat catttacaaa tgagaacaaa cgttatcgct
     9121 acaatcggac cctcttccat tgataaagat attcttggac aacttatatc tgaaggtgtg
     9181 gatataatta gaataaatac ttcatacgga gatattgagc aatacaaaaa ggttctccaa
     9241 aatcttaaag aggtagaccc caactccaga gtaaaaaaga tgattgatat taaaagtgaa
     9301 gatattctca atttttgtga tgaaaataaa atggatttca tagctgtttc ttttgctgaa
     9361 tcaaaggaac aaattgaaaa tataaaaaat aagcaccccg actgtgtact aatctcaaag
     9421 attgaatcag ttaaaggtgt tacgaattat cacgacatat tggaagtatc ggacggtgtc
     9481 atgattgctc gaggagattt aagcagtgcc gtaaccttgg agaaagtacc accactacaa
     9541 aaacaattta caaaggaagc gatcttaaaa aataaatatg ttattgccgc aactgaaatg
     9601 cttttatcaa tgactgataa accttatcca accaacgcag aggtgtctga cgtggccaat
     9661 gcagtttttg atggggttaa tgcagtaatg ttatccgaag agacagctgt aggtaaatac
     9721 cctgtagaat cagtaaagat tatggaaaaa ataataaccg aagcagaaaa ctacacaagg
     9781 gagcagaact taatttaagt caatcaaacg tttttaactt tatttctata aattgacagc
     9841 ttaacgaact cgcttgtaat aaaatatcca gcagcaatca ctatgattgt taataaatta
     9901 ctatatttaa gaggagtaaa tccaaatgtg ttctttccaa aaggtaagaa ggttactaca
     9961 aacgtactca cagctgccaa agaagttaaa aatataataa ttctggaagg ttttgaacga
    10021 ctttttaaaa taaatttctt tgtcctaatg gaaaagatta atatcagctc tgttaatata
    10081 ctttctataa accacgctgt ccttagtacc tcgggcttgc tcgacttaaa gatagcgaac
    10141 aataaaaggt cgaagacggt gctcacaatt cccagaaaag tcgccataat tactatttca
    10201 gtattgttat agacacatgg atcttttatt tcctccttct caacattatc tgtagcaata
    10261 gatatcattg gaaaatcgga aagtaagtta acaagtaaaa tttgaacagg aagcattgga
    10321 aggtattcaa tcaaaagtga ggctatagca acagcgtaaa aattaccaaa attagatgat
    10381 aatgtagctt taatatattt agatgtgttt acaaaggttt tccttccttc ttcaattccg
    10441 tcaataataa cttttaaatc ctgttgtaac aaaattaagt cagaggcttc tctggcaaca
    10501 tctgaagcac cgttaactac aatagccaca ttagcaagtt ttaatgcggg tgcgtcgtta
    10561 atgccatctc ccaagtaacc gactgtatac ttcttttgaa gtatctcaat aatttgaaat
    10621 ttctgttcag gagaaaccct agcgaaaaca tttcctttct caacagcaga tattttttct
    10681 tcgtaattca ttttttcaag atcaaaacca gaaattacct cgctagatga tttggcaata
    10741 ccaacctcgt aagcaactgc ccctgcaact tcagcgctgt caccagtaat tatttttatt
    10801 ctcacaccta actcctgtgc tttattaaca gcacttctag aacttatttt tacgggatct
    10861 gaaaaggaaa ttaaaccaac taattctaaa tttgattcct cgtcttcaac atcgtaatca
    10921 tctttactaa attctttcct ggcgacagta atcacccttc gtcctttttt accttgatct
    10981 aaaatccatt ctaaaatatt cttttcatct tcatttgaaa tatttgaaac atatggaagt
    11041 atactttctt gggcacctcg aacaataata tatctttttg actcaatttc tactaaaaca
    11101 ctatttcttc ttcgaacagg gttaaatgga ttttcattta acctttttac atttttcaat
    11161 tttgttaact caaaacgtcc tattttttta aataatgcca gatcaaatga attgtttgca
    11221 gttttctttt tcccaaaaac atctcccgct aatgcagagt agagaagtgt tttatcctta
    11281 tcttccgaaa atatttccga aactgaaaga ctattttcag ttaaagtacc tgtcttatca
    11341 ctacataaaa tttctatgct acccagatct tctatagctg ataatctttt agctactact
    11401 ttctttttag ctaattttaa agcaccttga gttaatgaaa aagtggtaac taaaggaagt
    11461 gcttcgggga tcactcctac agctagtgct atagaaaaaa gtatgagttc aggtatagca
    11521 atatcttgac ctttaataaa aaggtgggcc aaaaaaacta caacaagtgt tacagatgtc
    11581 agtattaaaa tgaacttact aaatttatta atgtgttttt cgaaaccact tgtatcagat
    11641 gtctcaaccg tcaatttagt tattttccca atctctgtgt ttttgcccgt gaatatgact
    11701 atgccttttg agttaccttc tgagactgtc gttcccataa atacaatatt gctagcttta
    11761 taaatttggg tttccttttc tttaagtgga gttgaaattt tcttaacggg aactgactca
    11821 cctgttaaag aagactcgtc gacatagaaa ttgtcttcca taactaaacg aatatccgcg
    11881 ggtactatgt caccaacctc caagagcacc acatctcccg gtactaaatc agtagatgaa
    11941 atataccgta cttgatctga tcttataact cttacctgtc tatcaactaa attttttaaa
    12001 tatgaaagtg ctttctgaga tttaaattct tgataaaacc ctaaacctgt atttataacc
    12061 acaaaaagaa gaatcattag tgaatcgaaa cgattattaa gtaaaaaaga aattacagac
    12121 gctgctatca acaggtaaat gaaagctgat ttaaattgcc ttaataaaat atcaaaccac
    12181 gtagattcgt tagctcgaag agtgttagga ccgtatctac cttttctaat attagctttt
    12241 tgaaatgaga ggcctttttc aatatcagta ttaaattctt cacataatgt ttgattggtt
    12301 ttagttgtgt attcagaaaa tttcataggc agttaaataa attttacact tttaaattaa
    12361 actaatacac acagttattt tttgaagggg ataggtggta tttatagact cgagtagtta
    12421 agatgcattg cttctttagt tctttccaaa gtcttttgtg ccaaattacg agcaaacacg
    12481 gtaccttcat ctaaaattcc tttgatatct tctttttcgt gtttttttct actttctctg
    12541 ataggctcta aaaactcctg aatagatttt tccaactttt ctttaacctc gacgtcacca
    12601 accgttcctt ttttgtatcg attttttaaa tcttcaacct cttctttatt tatattaaaa
    12661 acgtcgtgat aaataaaaac cggattacct tcaactgtac ccggatcatt aggatgaatt
    12721 ctttttgggt ctgtatacat agttttaatc atttttttaa cagtattttc gtcatctttt
    12781 aagaagatag cgttatttag ggacttggac atttttgctt gaccatcagt tcccacaagc
    12841 ctcccaattt taccaacaag tgcatcacac tcaacaaaag ttggagagta agttctatta
    12901 aatgctgaaa caatgttatt ggtatctcta aggtggggaa cctggtcctc tcctacggga
    12961 actaaaatag gttcttttga ttttttagga tcagtggatg aaaaaagtat atccgcagcc
    13021 tgactaactg ggtagtacat aaaacctgta gaaatatttt ttcctagata ttttgattct
    13081 gtttgaattg tggggttact ctgcatccat gtaaaaggtg taaccataga taaatacacc
    13141 gtaagctcgg tgagttcggg cattgcactt tgcaaaataa aatgaacatt ttctttacga
    13201 ggattaaggc cgattgataa aaaatctaaa actacttccc gtacggattc ctcaattaat
    13261 ttaggattat cggcatgagt agttaaagcc tgaatatcag ctatcatgaa aaaacattcg
    13321 tatttatcct gaagttcaac ccagtttttt aaggcaccta catagtggcc aaggtgtagc
    13381 ggacctgtag gtctgatccc tgtcatcaga ttacttaatg tattctttgg ttctgaaatc
    13441 atatataaat tttacactat taaaaccatt ttattaatat taagaggtat aaaagatagt
    13501 ttaagcaaat agggaagtcg gttattataa agataagctg gcaccggcgg gaggaatcga
    13561 acctccacca gcggttttgg agaccgctac tctaccacta agctacgccg gtaaattaat
//
LOCUS       DAOTFA010000007        12529 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate AMR_MDS_3115
            NODE_3526_length_12529_cov_6.979798, whole genome shotgun sequence.
ACCESSION   DAOTFA010000007 DAOTFA010000000
VERSION     DAOTFA010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868139
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (wastewater metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 12529)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12529)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:36:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 536
            CDSs (total)                      :: 521
            Genes (coding)                    :: 520
            CDSs (with protein)               :: 520
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12529
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3526_length_12529_cov_6.979798"
                     /isolate="AMR_MDS_3115"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..402
                     /locus_tag="P5014_00565"
     CDS             <1..402
                     /locus_tag="P5014_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006903015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRY56637.1"
                     /translation="TPEEVAKVMGIEPEKAHEIIKISQNTTSLETPVGKEEDTRLKEF
                     IEDEATLSPFEAASRELLKDHIGDVLHTLNAREKRVLELRFGLKDGRTRTLEEVGKEF
                     GVTRERIRQIEAKALRKLRHPSRSKKLRDYL"
     gene            451..2433
                     /locus_tag="P5014_00570"
     CDS             451..2433
                     /locus_tag="P5014_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56638.1"
                     /translation="MQGIIYLDKNRKIVGRDQENSESNNLNESEKEEHSSDSGVYKAN
                     ELSKEDENQMVWKPGFLQPKEKLEEEADKLSEYKNFTKPPVQTKPSFVEEVSIEDNKI
                     QEDINNMPSNFSEPFKKYLVDFKHSVKEENKRSSNPIYVDEIASKVAKVYETVRRIID
                     WKEEHLIRRAAIERVLKRRFVSKVYGISIIPDVKPVEAAEPFILELIRSGYFPNGKID
                     KDKISDIQNCLEKYLYILNNAVVINDEEAKTKKEQKAGVKKKINFYNWVIEIAACEIE
                     EILDAPLREYALMNLMTDSLLQRIKIIPENEISEEDKYIQTYISVHRTLYNLDKPITI
                     FHLLKYKYPEFFTSSEYLPEFTSKIFDVWNEIEDYLNYKKGSEFFRVAEKYDAAYLLI
                     GDSMNSMEKEVEIIEKKVSDTGVFIQAVENKYNERLATLKKRLSRLAIFSTLSIFVAG
                     LASLLLFEIPIASWVRGYFSPWSIVADLAIPTALMFILVWMIKPPSGNNLETVKEEVL
                     KVVYKVDELDIYEVRLKKKIKKFLNFIFAIIYLTGGMVSLYGIYWVFKIADVPWTSLY
                     INTGNVAMVVFSAMVIRQRSRELTIVEKENIIEFILDFFSIPLAKIGGWISAKWKEYN
                     IVSVFFIALVDFPFSSFVAMIESWRNFIKEKRSELN"
     gene            complement(2472..2945)
                     /locus_tag="P5014_00575"
     CDS             complement(2472..2945)
                     /locus_tag="P5014_00575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020264044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HRY56639.1"
                     /translation="MKNKKILTYIGFILVCQMAGIIGSLFTAPAVKGWYTTINKSPLN
                     PPSWVFAPVWTTLFIMMGVAVSIIWLSGKNETRSKALKVFFLQLFLNTLWSIIFFGLK
                     NPPLAFIEIVVLWFAILYTIILFRKIDKKASYLLIPYILWVSFASVLNLSIAILN"
     gene            3000..3956
                     /locus_tag="P5014_00580"
     CDS             3000..3956
                     /locus_tag="P5014_00580"
                     /inference="COORDINATES: protein motif:HMM:NF013832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56640.1"
                     /translation="MSAYLIKFFFGIIILLLSTQLFVKLAVKLSEVFRLSPLVIGMLV
                     VSFGTTLPELSVSITSSSSGSVGLAAGTILGSTIVNFILVLPAAILLGKDIRIGTTKT
                     QKNAIVLIIATILFVLLQQLPVPPLVSGLILLLGLLVSNFLEYRWGALGRLQEDKTVI
                     DLFKKRKITPDLIFGLFFSVICVTLGGLLVVRSMEDLAVISGYSTTVLGLTLTGIATS
                     LPDIFTAVMSQKNDEDKLTIGHITGGSLYNLLFIGGITNVLGADMNLGMIETLFLFGT
                     VFLFLLIIRFYKDKNIPKWVGLVLLLIFFLYIYTTSFLGKNF"
     assembly_gap    4102..4201
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4202..6253
                     /locus_tag="P5014_00585"
     CDS             <4202..6253
                     /locus_tag="P5014_00585"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HRY56641.1"
                     /translation="GADDVFDSALKEAGVIRCNSLEDFFDLSKAFSWANLPSGSRVAI
                     ISNAGGPAVISADSVVENGLEMADLGEDTRKKLLEVLPRSASIMNPVDVLGDALAQRY
                     ADASEIVLRNENCDALLVLLTPQIMTQIKKTAEMTGAVSEKYRKPVLCSFIGGSLVSE
                     GEKILNNLHIPSFRFPERAIYAIGKMREFKKRQEENATTQMDIYQVLNFDVMPEKVNK
                     LVKEAIDKKQPALNNIESDIVISAIGINTPPTLGVETLEEAKKFAIVQGYPLVLKLSS
                     PGLLHKKNVGGVVLDIKNEDELEDAWEILERKKQSLPSEVLSTTNFQVQKEVPSGTEV
                     IVGVKSDPTFGHFMLFGAGGSLAELISDKNIALLPMDLNKAKKLVEGSKIFKSLKGKD
                     GKTDYALEKLYELMVKVSKVVEGEPGIQEIEINPVIININDVWAVDTKVILRENKQMP
                     QGPKFKTAKVLKAENLTPKMHFFEFEAEEEFKVIPGQYISVKVSDSRVNCYSVAGFKN
                     PNRFNLLVDSTPGGPGSKFFESLKVGDKMTFLGPFGNFILKQDEDVDNMLFFATGCGT
                     APLKFMLESALKEGKKDRKIKLYLGVNNYCDVFFKDYFSDLQKEYENFSYEIAINSPD
                     PKWDGPVGFITELVKKDFPDASKCSAYLCGNKFMIEDVSKVLIERGCPEERVYTEKYG
                     K"
     gene            6243..>7552
                     /locus_tag="P5014_00590"
     CDS             6243..>7552
                     /locus_tag="P5014_00590"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HRY56642.1"
                     /translation="MVNKDDVVSVKIGGPAGTGVKSVGLMLAKIAARSGYQICDNTEY
                     PSLIRGGHNVMQVNFSEREVTAPRVKSDLLIAFDQLTIDLHFSEIESGGGIIFDSDSK
                     MDISKVSKDISLYGIPLKKLAFEAGEKNIFLNIVALSSAVGLLGGNIETFKKLIEEEY
                     GDKGEKIIEADKKAADLGYNYVLENFKDDLHEYMTVIDSFSSPAPYMVISGNEAAALG
                     AISAGMQFAAIYPMSPINNILHVLAANQEKFGYIYKQPEDEISAINMAIGASYAGVRS
                     MTATSGGGFCLMTEAYGLAGITETPLVIIEGMRGGPATGLPTWSGQGDLQMILHSHQD
                     EFSRIVLAPGDVEETYRLTAKAFDLADKYQTTAVVLIDKNICENDRTVMFPDVSNIKI
                     DRGKLIKEKVEDYKRYEFSDDGVSTRSIPGVGNFFIANSDEHDE"
     assembly_gap    7553..7652
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <7653..8140
                     /locus_tag="P5014_00595"
     CDS             <7653..8140
                     /locus_tag="P5014_00595"
                     /inference="COORDINATES: protein motif:HMM:NF028456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HRY56643.1"
                     /translation="IPGVGNFFIANSDEHDEYGYSSEEVDMRNKMMNKRMQKLTSCSK
                     NDMPAPEVYGPENADITILSWGSNKGSILEAIKSFSNVNYVHVTWMNPFPAEFLSKFL
                     SKAKHIVDIECNYTGQLADIVKEKTGIDIKDRYLRSDGRVIYPEEIIEKLNSVKGVKK
                     I"
     gene            8137..8991
                     /locus_tag="P5014_00600"
     CDS             8137..8991
                     /locus_tag="P5014_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011025335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRY56644.1"
                     /translation="MTLEELQTPCTPNWCPGCGNFNIWAAFKNAAVKEGWDNTNTALV
                     AGIGCHGHILNFVKLTSFEGLHGRAIPVASGLKLANNRLNVFAHVGDGDCLGEGGNHF
                     IHAARRNHNITVLLHDNALYALTAGQTSPATGKGVKTKSTPAGNPDEPIYPLALAIAS
                     GATFVARVYSGDMPKLTELIIEANKHKGFAVIDILQPCVTFNKICTHAMFLQNMYYLG
                     KDYDPKNKIKALEKAYEFGEKRIPVGIFYKEEKPSLEDGYSQISAIPLVDQEPKLDSL
                     TDLFKKYT"
     gene            9038..9283
                     /locus_tag="P5014_00605"
     CDS             9038..9283
                     /locus_tag="P5014_00605"
                     /inference="COORDINATES: protein motif:HMM:NF018595.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HRY56645.1"
                     /translation="MEEKEVQIGRYKIKVLRDVCISAASCVAFSPAVFELDGESKAVI
                     KDGATDEETNILLAAQSCPTGAIIITDAETGERVWPK"
     gene            complement(9357..10160)
                     /locus_tag="P5014_00610"
     CDS             complement(9357..10160)
                     /locus_tag="P5014_00610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56646.1"
                     /translation="MKKFLMVLITIMVAVSFSFGNFVEVRAQAPYPEIKVTYFQATPQ
                     ALQPEVRNSGTLYLELNLNGFDLEDLDGGRISIQNRDGTVHKISNIWSCQDQLCTWSA
                     YIPIEAVDIWVDVLGSIEWEGWGPWEIGYNDGLRKKEDVTQVTFSINDPLYNDWEGLY
                     NPTWNWAVVGNPEWAANQWFVNNRVDITIFTNCFGHGEYHNGLFQWEDVPPFPSKFGN
                     GVYREWLKEPIAPVQWEVKGTTGDWWTQSGETLGPVCWNSLIFLPLINK"
     gene            10370..10771
                     /locus_tag="P5014_00615"
     CDS             10370..10771
                     /locus_tag="P5014_00615"
                     /inference="COORDINATES: protein motif:HMM:NF013877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56647.1"
                     /translation="MKVYIFGNEDLDFDNKAILVAKKFKKTIENCDFIYVKPNQDLPF
                     VDREEIVIMDTIEGIEEITLITDGEISNTGFEKRTTVHDFDLGFQLKYLKKIGKLGKV
                     TIIGLPVAKDVDYLRIQSILRKLVAQDMQGS"
     gene            complement(10711..12012)
                     /locus_tag="P5014_00620"
     CDS             complement(10711..12012)
                     /locus_tag="P5014_00620"
                     /inference="COORDINATES: protein motif:HMM:NF012592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe hydrogenase subunit alpha"
                     /protein_id="HRY56648.1"
                     /translation="MHSGNITIENITKIEGSAGLDVTIENGKVTDVKFKIQDSRRFYT
                     DAVKKKPLISAPSFLSRICGTCSVAHLFATIKAIENSQGIEVTEQTKTLRRLAYDALI
                     IRDHALHLYFFVLPDLLGLDSILDIPDDHDDLGHTLLHDSFNIKQLGTDISEAVIGAA
                     IHAPIPTIGGFLKLPDESKFPELIERLEKIRPQVLRGIKAFYDYGASLEQDVDYVALR
                     NENKFDFLEGTLISLDGRKIPPEKFLEHFDVVVVPYSQSEGYVFSDNHGNYVLGALAR
                     INLNKDQLNLKTIKDTKEFLSVFPSKNIYHNNLAQAIEILHCVDDAIDILKNLKIVDE
                     KPIKKPLKAGVGIGVVEAPRGTLYHKAVIDEKGIVVDYDVIVPTSQNQIAIENDLKKY
                     FNENLDKDEEKLKFDAESIIRAYDPCMSCATNFLKIDWIRK"
     gene            complement(12015..12509)
                     /locus_tag="P5014_00625"
     CDS             complement(12015..12509)
                     /locus_tag="P5014_00625"
                     /inference="COORDINATES: protein motif:HMM:NF013242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56649.1"
                     /translation="MNSDQNKKLAIGWFCFTCSEDSTILFTELLNKYFDEWKKIIDFK
                     HIRPLKTKNELKDLDVAFVEGAISSKMQEGEVNKIRENAKYVVAVGQCACTGMPSASR
                     NAFEEGDIDDRIRWYLEHFDYNKKVQKLEDVVKVDDKIEGCPMNVTKFLSVMDKYLKL
                     FNII"
BASE COUNT         4051 a         1933 c         2390 g         3955 t
ORIGIN      
        1 actccagagg aagtggcaaa agttatgggt atcgaacctg aaaaagctca cgagatcata
       61 aaaatatctc aaaacactac atctttggaa actcctgtag gtaaggaaga ggacacaaga
      121 cttaaggagt ttattgaaga tgaagctact ttatcccctt ttgaagcagc tagcagagag
      181 cttctaaaag atcatatcgg ggatgttttg catacattaa atgcacgtga aaaaagagtc
      241 ttggagctta gatttggttt aaaagatgga agaacaagaa ctcttgaaga agtcggtaag
      301 gagtttgggg tcacacgaga aagaattaga cagatagaag ccaaggctct aaggaaatta
      361 agacatcctt cgagaagcaa gaagttaaga gattatcttt gataataacc gagaaaaaat
      421 cctataaaag atgtataata acctataatt atgcaaggta ttatctactt agacaaaaat
      481 agaaaaattg ttggtcggga ccaggaaaat tccgaatcta ataatttaaa tgaaagcgaa
      541 aaagaagaac attcgtctga ttcgggtgtg tataaagcta acgagttgtc taaagaggat
      601 gaaaatcaga tggtttggaa acccggtttt cttcagccta aagagaagtt ggaggaagaa
      661 gcagataaac tatctgaata taaaaatttc actaaacctc ctgtgcagac caagccctct
      721 tttgtggagg aagtgtctat tgaagataat aaaattcagg aagatatcaa caacatgccc
      781 tccaattttt cagagccttt taaaaaatac cttgtagatt ttaaacatag tgttaaagag
      841 gagaataaaa gatcatcaaa tcctatatat gtagatgaga ttgcatcaaa ggtagcgaaa
      901 gtttatgaaa cagtaagaag aataattgat tggaaagaag aacatcttat tagaagagcg
      961 gcaattgaaa gagtccttaa aagaagattt gtttctaagg tttatggaat aagcattatt
     1021 ccggatgtaa aacccgtgga agccgcagaa ccttttattt tggaattaat aagaagcggg
     1081 tatttcccta atggtaaaat tgataaagat aagattagtg atattcaaaa ttgcttggaa
     1141 aaatatcttt atatattgaa taacgctgta gtgataaatg atgaggaagc taaaaccaaa
     1201 aaagagcaaa aagcaggggt taagaaaaaa ataaatttct ataattgggt tattgaaatc
     1261 gctgcttgtg aaatagagga gatcttggac gcaccgctga gagaatatgc cttaatgaat
     1321 ttaatgacgg attctttgtt gcagaggata aaaataattc ctgaaaatga aatttctgaa
     1381 gaagataaat acattcaaac ttatatatct gttcacagga ctttgtataa tttagacaag
     1441 ccgattacaa tttttcatct attaaaatac aagtatccag aatttttcac ctcatcagaa
     1501 tatttacctg aattcacttc aaaaatattt gatgtttgga atgaaattga ggattaccta
     1561 aattataaaa aaggttcaga atttttcaga gtcgcagaaa aatacgatgc tgcttattta
     1621 ctaataggtg actcgatgaa ttcaatggaa aaggaggtag aaataattga gaaaaaggtt
     1681 tcggacacag gtgtttttat tcaggcagtt gaaaataaat acaacgaaag attggctact
     1741 ttgaaaaaaa gactatccag gcttgcaata ttttctacgc tttcaatttt tgttgccggt
     1801 ttagcgagct tgcttctttt tgaaattcca attgcaagtt gggttagggg ttactttagt
     1861 ccgtggtcaa tagtagctga cctcgctatc cctactgcat taatgttcat ccttgtctgg
     1921 atgattaagc ctccttcagg aaataacctg gaaacggtaa aagaagaggt tttaaaagtt
     1981 gtgtataaag tagatgagtt agatatttat gaagtcaggc ttaagaaaaa aataaagaaa
     2041 tttcttaatt ttatatttgc aataatttac ctaacaggag gtatggtatc tttgtatggt
     2101 atatattggg tttttaagat cgcagatgtt ccttggactt ctctttatat aaacactgga
     2161 aacgtagcta tggtagtttt ttcggctatg gtgattagac aaagatccag agaattaaca
     2221 atagtagaga aagaaaatat tattgaattt attttggatt tcttttcaat acctctcgca
     2281 aaaataggtg ggtggatatc tgcaaagtgg aaagaatata acattgtgag tgtgtttttt
     2341 attgccttag ttgattttcc tttttccagt tttgtcgcaa tgatagagag ttggagaaat
     2401 tttataaaag agaaaaggtc agaattaaac tgacctcagt tctatttact caaaaataca
     2461 tttcaatttt cttaatttag tatagctata gatagattaa gaacagaggc aaatgatacc
     2521 cacaagatat aaggaatcag gaggtacgaa gcttttttat ctattttcct aaataaaatt
     2581 attgtataga gtatcgcaaa ccaaagtacg actatctcga taaaagctag gggtgggttt
     2641 tttaaaccaa aaaatattat tgaccacaaa gtgttcaaga aaagttgtag aaaaaatact
     2701 ttgagtgctt tagaccttgt ttcgtttttt ccggaaagcc atataataga aaccgcaact
     2761 cccatcataa taaaaagggt tgtccatacg ggagcgaaaa cccaactagg tggatttaaa
     2821 ggtgatttat ttatggttgt gtaccagccc tttactgcag gcgcggtaaa taaagatcca
     2881 ataattccgg ccatttggca aactaatata aatcctatat acgtgagtat ttttttattt
     2941 ttcatatcta aattgtataa tttttggcta ttttaaaagg atgccatata attgactaca
     3001 tgtctgccta tttaataaaa tttttcttcg gaataattat tttactttta tctacacagt
     3061 tatttgtaaa acttgcagtt aaactttctg aagtttttag gttatctcca ttagtaatag
     3121 gtatgttagt ggtgtctttt ggcactacat tacccgaact ttcggtttcg attacatctt
     3181 caagctcggg aagtgttggt ttggccgcag gaaccattct tggttcaacg attgtaaatt
     3241 ttatattagt attacccgcg gcaattttat taggaaaaga tataagaatt ggtactacaa
     3301 aaacccaaaa gaatgcaatt gtattaatta tcgcaacgat tttgtttgtt ttattacagc
     3361 aacttcctgt cccaccactt gtttcgggat taatcttgtt gttggggttg ttagtttcaa
     3421 actttctcga gtataggtgg ggagcgttag gaagacttca ggaagataaa accgtgattg
     3481 atttatttaa aaaaagaaaa ataacccccg atctgatttt tggtttattt ttttcggtaa
     3541 tttgcgttac tttaggtgga cttttggtag ttagatccat ggaagatttg gcggttatat
     3601 caggatattc caccactgtt ttaggtttaa ctttaacagg aattgccact tccctgcctg
     3661 atatttttac cgctgtgatg agtcaaaaaa atgatgagga taagttgacc atagggcata
     3721 tcactggcgg tagtttatat aatcttttat ttattggcgg gattactaat gttttaggag
     3781 ctgatatgaa tttgggtatg attgagacac tttttttatt cggaacagta tttttatttc
     3841 ttttaataat aagattttat aaggataaaa atatacctaa gtgggtaggt ctggtgttac
     3901 tgttaatttt ctttttgtac atatacacca cttctttttt aggtaaaaat ttttaattta
     3961 aattttacct cgctctcgtt ttatgtttct gataccccga atttattaat caggcattga
     4021 cgcagtaatc aaaaattaat agactaagtt aatgcccaaa aacctaaatg ttctattttc
     4081 accaaaatct atagctattg tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4141 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4201 nggggctgat gatgtttttg attcagcttt aaaagaagct ggggttataa gatgcaattc
     4261 cttagaggat tttttcgatt tatcaaaagc attttcatgg gccaacttac cttcgggatc
     4321 tagagtggca attatttcta atgccggagg ccccgcggtt ataagtgctg acagtgttgt
     4381 agaaaatggc ttagagatgg cggaccttgg cgaagatacc agaaaaaaat tattggaagt
     4441 acttccaaga tctgcgagca ttatgaatcc ggtagatgtc ttaggtgatg ctctagccca
     4501 aagatatgct gatgcgtctg agatagttct tagaaatgaa aactgcgatg ctttattagt
     4561 tctgcttact cctcaaataa tgacccaaat aaaaaagacc gcggagatga ctggtgctgt
     4621 ttccgagaaa tatcgcaagc cggttttgtg ctcatttata ggaggatctt tagttagcga
     4681 gggtgagaaa attttaaata atttgcatat tccgtcattt aggtttcccg aaagagctat
     4741 atacgctata ggtaaaatgc gagagtttaa gaaaagacag gaagaaaatg caacaacaca
     4801 gatggatatt tatcaagttt taaattttga tgttatgcct gaaaaggtaa ataagttggt
     4861 aaaagaagct attgataaaa aacaaccggc acttaacaat attgaatcgg atattgtaat
     4921 ctcagcaatc ggtataaaca cacccccaac tttgggtgtt gagaccttag aagaagccaa
     4981 aaaatttgca atagttcaag gttatccctt ggttttaaaa ctatcttctc ctggattact
     5041 gcataagaaa aatgtaggtg gggttgtttt ggatattaag aatgaagatg aacttgaaga
     5101 tgcttgggaa attttagaaa gaaaaaaaca aagtctgcct tccgaagtgc tatctacaac
     5161 taactttcaa gtacaaaaag aagttccatc ggggaccgag gtaattgtgg gagtaaaaag
     5221 tgacccaaca tttgggcact ttatgctttt tggagctggt ggttcattgg cagaattaat
     5281 ttctgataaa aatattgcgc tccttcctat ggatcttaat aaggcaaaaa aacttgtaga
     5341 aggttcaaag atatttaaat cattaaaagg taaggatgga aaaacagatt acgccttaga
     5401 aaaactttat gaacttatgg ttaaggtgag taaagttgtt gagggtgagc cgggaatcca
     5461 agagattgaa attaacccgg ttattataaa tattaatgat gtgtgggcag tggacacaaa
     5521 ggtaatttta agagaaaata aacaaatgcc ccaaggtccg aaatttaaaa ccgcaaaagt
     5581 attaaaagct gaaaacctca ctccaaaaat gcattttttt gaatttgaag cagaagaaga
     5641 atttaaagtg ataccgggac aatatataag tgtaaaggtt tcagattcca gagttaactg
     5701 ctactctgtt gctggtttta aaaatcctaa taggtttaat ttacttgttg attcaacccc
     5761 aggtggacca ggatctaagt tttttgaaag tttaaaggtt ggggacaaga tgaccttttt
     5821 aggacctttt ggaaatttca tattaaaaca agatgaagat gttgataaca tgcttttctt
     5881 tgctacaggc tgtggtacag ctcccttaaa atttatgtta gaaagtgctt taaaggaagg
     5941 taaaaaagat agaaaaataa aattgtatct tggggttaat aattattgtg atgttttctt
     6001 taaagattat ttcagtgatt tacaaaaaga atacgaaaac ttttcttatg aaattgcgat
     6061 aaatagccca gatccaaaat gggacgggcc cgttggtttt ataactgaat tggtaaaaaa
     6121 ggatttccca gatgcttcta aatgttccgc ttatttgtgt gggaataaat ttatgattga
     6181 ggatgtttca aaagttttaa tagaaagagg ttgcccggag gaaagggtat atacggaaaa
     6241 atatggtaaa taaagatgat gtggtatcag taaaaatagg aggtcccgcc ggaacaggtg
     6301 ttaaatctgt aggtttgatg ttggcaaaaa tagcggctag atcaggttat cagatttgtg
     6361 ataatacgga atacccgtct ttaattagag gtggtcacaa tgttatgcag gtaaattttt
     6421 cagaaagaga agttacggca ccaagagtta agtcagatct tttaatagcc tttgatcaac
     6481 tgacaataga cctgcatttt tcagaaattg aaagcggggg aggaataatt tttgattcag
     6541 attccaaaat ggatatctca aaagtttcaa aagacatttc actttacggt attcctttaa
     6601 aaaaactagc atttgaagcc ggtgaaaaaa atatattttt aaatattgtg gctttatctt
     6661 ccgcagtcgg tcttttaggt ggaaatattg aaacatttaa aaaattaatc gaagaagaat
     6721 acggtgataa aggagaaaag attattgagg ctgacaaaaa agccgctgat ttaggataca
     6781 actatgtttt ggaaaatttt aaagatgacc ttcacgaata tatgaccgtg attgattctt
     6841 ttagctctcc cgcaccttat atggttataa gtgggaatga ggcggccgct ttaggtgcta
     6901 tctccgcagg tatgcagttt gccgcaatat atccaatgtc tcccatcaac aatatcctcc
     6961 atgtcttggc cgccaatcag gaaaaattcg gttacatcta caaacaaccg gaagatgaaa
     7021 tttccgcaat taatatggca ataggtgctt cctacgcagg agttcgttct atgacagcaa
     7081 cttcgggggg tggtttttgc ttaatgacag aggcatatgg tcttgcagga attactgaaa
     7141 cacctttggt aataattgag ggaatgagag gcggtcccgc aacaggcctt cctacttgga
     7201 gcggacaggg agatcttcaa atgatacttc actctcatca ggatgaattt tcaagaatag
     7261 ttttagctcc cggagatgtt gaagagactt accgtcttac ggcaaaggcc tttgatttgg
     7321 ctgataagta tcaaactaca gcagtagttt taatcgacaa gaatatttgt gaaaatgata
     7381 ggactgtaat gtttcctgat gtttcaaata taaaaattga taggggtaaa cttataaaag
     7441 aaaaagttga agattataaa aggtatgaat tttcagatga cggtgtttcc accagaagta
     7501 ttccgggagt tgggaatttc tttatagcca attctgatga acacgacgaa tannnnnnnn
     7561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngtattccg ggagttggga atttctttat
     7681 agccaattct gatgaacacg acgaatacgg ctattcttca gaagaagtag atatgagaaa
     7741 taaaatgatg aataaaagaa tgcagaagct tacttcgtgt tctaaaaatg acatgcccgc
     7801 ccctgaagtt tatggccctg aaaacgctga tataacaata ctctcgtggg ggagtaataa
     7861 aggaagtatt ttagaagcta taaaaagttt ttcaaatgta aattatgttc acgttacttg
     7921 gatgaaccct ttccctgcag aatttttatc taagttttta tccaaagcta aacatattgt
     7981 tgatattgaa tgtaattaca ctggtcagtt agcagatatt gtaaaagaaa aaacagggat
     8041 tgatattaaa gatagatatt taaggtctga cggaagagtg atttatccgg aggaaataat
     8101 agagaaatta aattctgtga agggagtgaa aaagatatga cattagaaga attgcaaacc
     8161 ccttgtactc ctaattggtg tcccggttgt ggaaacttta atatttgggc agcttttaaa
     8221 aacgcagctg taaaagaggg ttgggataat accaacacgg ctttggttgc aggaataggt
     8281 tgtcacggac acattttaaa ttttgtaaaa cttacatctt ttgaaggttt acatggtagg
     8341 gcaattccgg tggcttcggg attgaaattg gcaaataaca gattaaatgt ttttgctcat
     8401 gttggtgatg gagattgttt aggtgagggc ggtaatcact ttattcatgc cgcgagaaga
     8461 aatcataata ttacggtact attgcatgac aacgctttat acgcattaac tgccggtcaa
     8521 acgtctcccg caacgggaaa aggtgtgaaa actaaatcta ctcctgcagg aaaccccgac
     8581 gaaccaatct accccctagc tttagcaata gcctcggggg ccacgtttgt cgctcgggtt
     8641 tattcaggag atatgcctaa acttacagag ttaattattg aggccaataa gcataaaggt
     8701 tttgcggtaa tagacattct tcagccatgt gtcactttta ataaaatttg cacacacgca
     8761 atgtttcttc aaaatatgta ctatcttgga aaagactatg acccaaaaaa caaaataaaa
     8821 gctctagaaa aagcttacga atttggggag aagagaattc ccgttggtat tttttataag
     8881 gaggagaagc cgtctttaga agatggttat tctcagattt ctgcaattcc tctggttgac
     8941 caagaaccaa aattagacag ccttacggat ttatttaaaa aatatacttg atagggtagt
     9001 atttaattag ttagaaaatt taaaaaggac tttaaaaatg gaagaaaagg aagttcaaat
     9061 tggaagatac aaaataaaag ttttaagaga tgtttgcatt agcgccgctt cttgtgtagc
     9121 tttttcccca gctgtttttg aattagacgg agagtcgaaa gctgttataa aagacggagc
     9181 aactgacgaa gagactaaca ttttacttgc agctcaatct tgccccacag gagcaattat
     9241 tattaccgac gctgaaactg gcgagagagt ttggcctaag taagaagttt ttaaaaaatt
     9301 aaggacaccc ttgttcatat atttatgaac tttaggtgtc cctttttgaa tttattttat
     9361 ttatttatca atggtagaaa aattaggctg ttccagcaaa ctggacctaa tgtttctcca
     9421 ctttgtgtcc accaatctcc agtagttcct tttacttccc actgaactgg tgctattggt
     9481 tcttttaacc attctcgata caccccattt ccaaacttgc ttggaaaggg tggtacgtct
     9541 tcccattgaa atagaccgtt atgatattca ccgtgtccaa agcagtttgt aaatattgta
     9601 atatcaactc tgttatttac aaaccattgg tttgcagccc attcgggatt cccaacaact
     9661 gcccaattcc acgttgggtt gtacaaacct tcccaatcat tgtacaaagg atcgttgatt
     9721 gagaaagtaa cttgggtgac atcttctttt ttacgtaacc catcgttata accaatctcc
     9781 caaggtcccc acccttccca ttcgattgac cctaaaacat ctacccagat gtcaactgcc
     9841 tctattggga tgtaagcact ccaagtacac aactgatctt ggcaagacca gatgttactt
     9901 attttgtgaa cagtcccgtc tctgttttga atagatattc tacctccgtc taaatcttcc
     9961 aaatcgaatc cgtttagatt aagttctaaa tagagcgtac cactgtttcg cacctcaggt
    10021 tgaagcgctt gtggggtcgc ttgaaaatag gtgaccttga tttctggata aggtgcttga
    10081 gctcttactt ccacaaaatt tccaaatgaa aaggatacag cgaccataat cgtgattaaa
    10141 accatcaaaa attttttcat ttttctcctt atttatggga ttgtattcat cgcttgaaat
    10201 tttagcggtg attttagtgc tttgttggat tatagcctaa aaaacctcgt cagtcaaata
    10261 ctacaggtct atgtctacgt cctatcttag atttacattg ggtttgcgaa catctaaatt
    10321 gctcgcgatc tgtattgcta aaagtcttta tcaaatgtaa aaagaaagta tgaaggttta
    10381 tatattcgga aacgaggatt tagattttga taataaagct attcttgttg ccaaaaaatt
    10441 taaaaaaacc atagaaaact gtgattttat ttatgttaag ccaaaccaag atcttccatt
    10501 tgtggataga gaagagattg taataatgga tacaatcgag ggaatagaag aaataactct
    10561 gataactgat ggtgagatat caaataccgg atttgagaaa agaaccaccg tacacgattt
    10621 tgatttaggt ttccaactta aatacttaaa aaagataggg aagttgggaa aagtaacaat
    10681 aataggttta ccggtggcaa aggatgtaga ttatttacgg atccaatcaa tcttaaggaa
    10741 gttagttgca caggacatgc aggggtcgta agctctgatt atactttccg catcaaattt
    10801 taatttttcc tcgtctttat ctaagttttc attaaaatat tttttaaggt cattctctat
    10861 ggctatttga ttttgagaag tagggacaat tacatcgtaa tcaacgacta tccccttttc
    10921 gtctatcacc gctttatgat atagagtgcc tctgggagct tctactacac caattccaac
    10981 cccagcctta agtggttttt taattggctt ttcgtcgaca atttttaaat tctttaaaat
    11041 gtctatcgca tcatcgacac agtgaagtat ttcaatagcc tgagctaaat tgttgtgata
    11101 aatatttttt gatggaaaga cagatagaaa ttcttttgta tcttttatag tttttagatt
    11161 aagttggtct ttatttaaat taattcttgc taaggctcct aaaacataat ttccatgatt
    11221 atctgaaaac acgtatcctt cggattgtga ataaggaact accaccacat caaaatgttc
    11281 caagaatttt tcaggtggta tttttcttcc gtctaaacta atcagagtac cctctagaaa
    11341 atcaaattta ttctcatttc ttaaagccac gtagtctaca tcttgctcaa gagaagcacc
    11401 gtaatcgtaa aaagctttaa ttccccttaa tacttgaggc cttatttttt ccaatctctc
    11461 tataagctct ggaaattttg attcgtccgg tagttttaaa aaccccccga tagttggtat
    11521 aggggcgtgt atagcggcac cgataactgc ctctgatata tcagttccga gttgttttat
    11581 attaaaagaa tcgtgaagaa gtgtgtgacc taaatcatcg tgatcgtcgg ggatgtctaa
    11641 aatagaatcc aatcctaata aatctggtag aacgaaaaaa tataaatgaa gggcatggtc
    11701 cctaattatt agagcgtcat aagctaatct tcttaaagtt tttgtttgtt ctgtaacttc
    11761 aattccctgc gaattttcta ttgctttgat ggtagcaaaa agatgagcaa cagagcacgt
    11821 tccgcatatt cttgataaaa atgacggagc agagatcagt ggttttttct ttacagcgtc
    11881 agtataaaat cgtctagagt cctggatctt aaatttcaca tcagttactt ttccgttttc
    11941 aattgtgacg tcaaggcctg cagagccttc gattttagtg atattttcga ttgtgatatt
    12001 tccgctatgc atttttatat tatattaaac aactttaaat atttatccat tacagataga
    12061 aattttgtaa cattcatcgg acatccctct attttatcat ctacttttac aacatcttca
    12121 agtttttgta cttttttgtt gtaatcgaaa tgctctaaat accatctaat tctgtcgtca
    12181 atgtctccct cttcaaaagc atttctgctg gctgaaggca ttcctgtaca agcacactgt
    12241 cctacagcta caacatattt tgcattttct cttattttat ttacttctcc ctcttgcatc
    12301 ttggatgaaa ttgctccttc aacaaatgct acatccagat ctttcaattc gtttttagtt
    12361 tttaatggtc taatgtgttt gaaatcaata atttttttcc actcgtcaaa atatttattt
    12421 aaaagttctg tgaataaaat tgtgctgtct tcactacatg taaaacaaaa ccacccgatt
    12481 gctaattttt tattttgatc agaattcata ttttctttat tctaatatt
//
LOCUS       DAOTFA010000008        11273 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate AMR_MDS_3115
            NODE_4054_length_11273_cov_7.070868, whole genome shotgun sequence.
ACCESSION   DAOTFA010000008 DAOTFA010000000
VERSION     DAOTFA010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868139
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (wastewater metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 11273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:36:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 536
            CDSs (total)                      :: 521
            Genes (coding)                    :: 520
            CDSs (with protein)               :: 520
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11273
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4054_length_11273_cov_7.070868"
                     /isolate="AMR_MDS_3115"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /gene="rplO"
                     /locus_tag="P5014_00630"
     CDS             complement(<1..428)
                     /gene="rplO"
                     /locus_tag="P5014_00630"
                     /inference="COORDINATES: protein motif:HMM:TIGR01071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HRY56650.1"
                     /translation="MNLKNNMELFNIKKLNNDKKSKRVGRGRGSGKGGHTVGRGQKGQ
                     KARKGAKPNLGFEGGQSPLYKKLPRIGGFKRPYITKSKALGVSLDIFNKFKDGEEITP
                     ISLVEKKVLKDIPSGGVKILSKGELKKKVVFKDFTYSEKA"
     gene            complement(412..888)
                     /gene="rpsE"
                     /locus_tag="P5014_00635"
     CDS             complement(412..888)
                     /gene="rpsE"
                     /locus_tag="P5014_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010024567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HRY56651.1"
                     /translation="MKKEERNKEQYSNTAKISEFEEKIIEIKRVSKKIKGGSAIGFSV
                     LVVVGNKNGKVGYGFGKAQNVADAIRKAINSAKKKMYDVKISGKTIAHEVTAKVGASK
                     VLLKPAPEGTGVIAGGSVRIVMEMAGVKDISSKVLGSNNKLLNVRCAIKALNELKK"
     gene            complement(885..1244)
                     /gene="rplR"
                     /locus_tag="P5014_00640"
     CDS             complement(885..1244)
                     /gene="rplR"
                     /locus_tag="P5014_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004101467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HRY56652.1"
                     /translation="MYHVKSRKTNREKRRLSIRNKIYGTFETPRLSVFRSNKDIYAQI
                     IDDEKSGTIVEFSSKKLKSAKGTTKTQKSFEVGKEISKLALEKGIKSVVFDRGGYRYH
                     GRVKAVAEGAREGGLKL"
     gene            complement(1247..1807)
                     /gene="rplF"
                     /locus_tag="P5014_00645"
     CDS             complement(1247..1807)
                     /gene="rplF"
                     /locus_tag="P5014_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016426362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HRY56653.1"
                     /translation="MSRIGKAPILIPQGVTVEIAGNEVIVKGLKGNLSFKFSDDVVVK
                     KEEDKINVEMIGKSKESPALWGTTRAMISNMVKGVTEGYEKKLELVGVGYRVKSVSPR
                     EISLTVGYSHPVDFKSPEGVELKVEDNTHITVSGFDKHLVGLTAAKIRKVRKPEPYKG
                     KGIKYADEVIRRKPGKAGKVGSGGAA"
     gene            complement(1811..2209)
                     /gene="rpsH"
                     /locus_tag="P5014_00650"
     CDS             complement(1811..2209)
                     /gene="rpsH"
                     /locus_tag="P5014_00650"
                     /inference="COORDINATES: protein motif:HMM:NF001109.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HRY56654.1"
                     /translation="MSLDRLSNMVSSIKNASMGGRDSLEIPFTSECESVAKVLKDRGF
                     VEEVKTFKKENSSAKMLKITLAKDGNVIKLSEAKRVSKPGRRAYIGYKEVKPVLQGLG
                     VMILSTSRGIMDAQEARKKKLGGEVICEVY"
     gene            complement(2211..2396)
                     /locus_tag="P5014_00655"
     CDS             complement(2211..2396)
                     /locus_tag="P5014_00655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002836123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HRY56655.1"
                     /translation="MAKEALKQKHSRKQKFSSREYHRCSICGNPRGYMRRFKVCRKCF
                     REMAHKGELPGVRKASW"
     gene            complement(2398..2958)
                     /gene="rplE"
                     /locus_tag="P5014_00660"
     CDS             complement(2398..2958)
                     /gene="rplE"
                     /locus_tag="P5014_00660"
                     /inference="COORDINATES: protein motif:HMM:NF000585.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HRY56656.1"
                     /translation="MNRLKEKYNKEIAKDMLKEFKLGNVMEVPSIKKISVNAGIGPFR
                     ENREAADFFASELSDIAGQKVFERKARKSEAGFKIKQNDIVGYTVTLRGDRMWAFLDK
                     LINIAFPRIRDFRGFSPDSFDENGNYSVGIREHVIFPEVNPNTTKGIRSLQVTVVFNG
                     KNIDQNRYLLKKLSFPFSESANIESK"
     gene            complement(2955..3272)
                     /gene="rplX"
                     /locus_tag="P5014_00665"
     CDS             complement(2955..3272)
                     /gene="rplX"
                     /locus_tag="P5014_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HRY56657.1"
                     /translation="MRLKKDDKVKVISGKDKGKVSKILKVLKDKNKIVVEGVNVVKKH
                     VKPGVISKEGGIISIEKPVDVSNVMYYDDSSKKVVKIGYKLVDGKKYRVSKETGEILD
                     KKI"
     gene            complement(3274..3639)
                     /gene="rplN"
                     /locus_tag="P5014_00670"
     CDS             complement(3274..3639)
                     /gene="rplN"
                     /locus_tag="P5014_00670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004568733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HRY56658.1"
                     /translation="MLPIGSYLKPADNSGAIMLKIVGIPGSNKRSARLGDYVTVVVKG
                     ASSTGTLKDHSMAKAIVIRTKKEVRRKDGSYIRFDDNAAIVVDKDKNMIGTRVFGPVA
                     REIRDRGYSKIVSLAKEVL"
     gene            complement(3641..3889)
                     /locus_tag="P5014_00675"
     CDS             complement(3641..3889)
                     /locus_tag="P5014_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014082369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HRY56659.1"
                     /translation="MSVKTFKGKVISNKMQKTVVVAVESPKKHPKYNKSIKNTTKLKA
                     RDDIGVMVNDEVIIEECKPFSKDVAFKVLSKISKEEQK"
     gene            complement(3886..4089)
                     /gene="rpmC"
                     /locus_tag="P5014_00680"
     CDS             complement(3886..4089)
                     /gene="rpmC"
                     /locus_tag="P5014_00680"
                     /inference="COORDINATES: protein motif:HMM:NF013027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HRY56660.1"
                     /translation="MIDIKEFRKKELDDLKKELLKVKKDFHKTISDVMQKKEKNIKKA
                     SQFRKDIARIQTIINEKLKEVQK"
     gene            complement(4086..4499)
                     /gene="rplP"
                     /locus_tag="P5014_00685"
     CDS             complement(4086..4499)
                     /gene="rplP"
                     /locus_tag="P5014_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HRY56661.1"
                     /translation="MMMPRKVKFRRQQRGTNKGISVRGSSISFGDYGLKVVDRCLLSY
                     RQLESARKAITHETKRGGKLWIRAFPDKPVAKKANETRMGSGKSNTDHYAAMVKPGKV
                     IFEMAGVTEDVAKKAFYKAASKLPIKTKFVRRDDQ"
     gene            complement(4513..5148)
                     /gene="rpsC"
                     /locus_tag="P5014_00690"
     CDS             complement(4513..5148)
                     /gene="rpsC"
                     /locus_tag="P5014_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012301384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HRY56662.1"
                     /translation="MGQKINPVGYRVGISKNWSSRWYTSKRDYANLLIEDKKIRDFLN
                     EKLSTAGLKNVEIERTENEVSILIKVSKPGVVIGRGGTGVEEIEKEIKKITKGKVKIT
                     AEGVKSSEIEAQLVGDYICRQLKRRVPYRRVVNFALSSAIDKGAKGIKIKLAGVLSGS
                     NTISRSETYKLGSVPAQTLRADIDYAQIHCQMLFGTIGIKVWIYKGEIELK"
     gene            complement(5148..5498)
                     /gene="rplV"
                     /locus_tag="P5014_00695"
     CDS             complement(5148..5498)
                     /gene="rplV"
                     /locus_tag="P5014_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009524378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HRY56663.1"
                     /translation="MYDVKITAKHMGARVSPKKVAIVLDLVRGRGVFEAKKILSFDRS
                     KSAKIVLKVVKSAEANAVNNSKMNKDKLFINRIWVSGGPSLKRMSPGAKGRVDPILKR
                     YSHIYVSLDERDQK"
     gene            complement(5500..5772)
                     /gene="rpsS"
                     /locus_tag="P5014_00700"
     CDS             complement(5500..5772)
                     /gene="rpsS"
                     /locus_tag="P5014_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HRY56664.1"
                     /translation="MSRSLKKGIYTDEKLMKKVEKALSGDKKPIKTWARNSTIVPNMV
                     GLTFEVHNGKKHISFFVTESMVGHKLGEFAPTRTFRAHSSKKGIAE"
     gene            complement(5775..6608)
                     /gene="rplB"
                     /locus_tag="P5014_00705"
     CDS             complement(5775..6608)
                     /gene="rplB"
                     /locus_tag="P5014_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015941735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HRY56665.1"
                     /translation="MIKSYKPTSEGLRTRKTLVKGVDKVRPEKSLTKGRSSNSGRSRG
                     KVTSRHKQRGAKKLYRFVDFKRDKFNIPAKVISIQDDPNRGPNIALLAYADGEKRYIV
                     APEGLEVGSTIISGENAEISLGNNVPLSKIPLGTSIHNIEINPGAGAVLCRSAGNQAQ
                     IIAKEGNYVNVKLPSGEVKRIPDVCTATIGVIGNIDKRNVDLGKAGRMRYLGRRPHVR
                     GIVMGDPHRDHPHAGKYSTSGIGIVAPLSPWGWKTKGVKTRKRVRTDYTIVSRRKGKK
                     K"
     gene            complement(6614..6907)
                     /gene="rplW"
                     /locus_tag="P5014_00710"
     CDS             complement(6614..6907)
                     /gene="rplW"
                     /locus_tag="P5014_00710"
                     /inference="COORDINATES: protein motif:HMM:NF004363.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HRY56666.1"
                     /translation="MRLSKSIIKPIITEKSYDHSSFGRYVFEVSVKATKGAIRSEIKD
                     LYGVDVTEVKTAILPGKSSRIGRTRLFTTPAKWKKAVVKLKEGQSIDLFPKDK"
     gene            complement(6904..7527)
                     /gene="rplD"
                     /locus_tag="P5014_00715"
     CDS             complement(6904..7527)
                     /gene="rplD"
                     /locus_tag="P5014_00715"
                     /inference="COORDINATES: protein motif:HMM:NF012782.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HRY56667.1"
                     /translation="MKVDVINLKGEKLEQIDLRDEIFGSDYNPTVLAQYIRVYQNNQR
                     QGTSSTKTRAEVSGGGLKPRAQKGSGRSRQGSIRSPIWVGGGVAHGPKPKSYKSQLSK
                     KMKGVALSSALSTRMKEGKVIIIDGVDIKKPNTKEFSQSLKKIKVSGSLSLVWLGENE
                     NMIRSARNIPEVNLVNVKSLGAYDVMRTKNIVFLKNAVLDFQERFSK"
     gene            complement(7538..7972)
                     /gene="rplC"
                     /locus_tag="P5014_00720"
     CDS             complement(7538..7972)
                     /gene="rplC"
                     /locus_tag="P5014_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012499636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HRY56668.1"
                     /translation="MEKIIGKKLNMTQLFGDNGVVVPVTIISCESDVSVDFENKDIVV
                     VGKSKGKGFAGVMKKWGFHGGPATRGQGIKGRSPGSIGSQTPGRVLKGKKMAGRAGNQ
                     RITIKGLKLVKVMPELKQVMVSGPVPGPRNSKIEIKFVESKK"
     gene            complement(8116..8442)
                     /gene="rpsJ"
                     /locus_tag="P5014_00725"
     CDS             complement(8116..8442)
                     /gene="rpsJ"
                     /locus_tag="P5014_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013181408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HRY56669.1"
                     /translation="MAKGKTQKDTKIRIKLKSYDSRIIDLSCSKIVDTAIRTGAQIVG
                     PVPLPTKIEKFTVIRGPHIDKRSREQFELRTHKRVIDVLNPTPSTVETLSKLSLPAGV
                     GISIKM"
     gene            complement(8495..9673)
                     /gene="tuf"
                     /locus_tag="P5014_00730"
     CDS             complement(8495..9673)
                     /gene="tuf"
                     /locus_tag="P5014_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018201501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HRY56670.1"
                     /translation="MTDFVRNKEHLNIGTIGHVDHGKTTLTSAITKVLADKGMASYRE
                     FNTIDNAPEEKERGITINITHVEYETEKRHYAHIDAPGHKDYIKNMITGAAQMDGAIL
                     VVSAPDGPMPQTREHILLARQVNVPAMVVFMNKCDMVQDKEILDLVELEIRELLNKYG
                     FPGDTTPIIKGSALKALEGDPEYVKKIEELMAAVDEFIPTPSRELDKPFLMPVEDVFT
                     ISGRGTVVTGRIERGKVELNEEIEIVGIRDTKKTIVTGIEMFKKSMKEGQAGDNVGVL
                     VRGIERTDVERGQVLAKPGSVKPHTEFDAEVYVLSKEEGGRHKPFFSGYRPQFYVRTT
                     DVTGETKLPEGVEMVLPGDHVKMTVKLITPVALEEGMRFAIREGGSTVGHGVVSKVIA
                     "
     gene            complement(9877..>11273)
                     /gene="fusA"
                     /locus_tag="P5014_00735"
     CDS             complement(9877..>11273)
                     /gene="fusA"
                     /locus_tag="P5014_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013274959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HRY56671.1"
                     /translation="KYLNGGELTIEEIKKAIRKMTVKAELYPIFCGASLSNKGVQKVL
                     DGVIEYLPSPKDTPDIKGINSNTGEEVSLSSDIDGPLAALAFKIQTDPYVGRLTYIRV
                     YSGKITAGSYVYNSTTGKKERLGRILLMHANHREEIKEVLAGEIAAGVGLEATTGDTL
                     CNEEHKILLDKISFAEPVIGIVIEPKTKADRDKMSQVIKKFLEEDPTLQIKTNIETGQ
                     TLLYGMGELHLDIIVDRMRREFNLEINTGKPQVAYRETIRKVVEQEGKYIRQSGGRGQ
                     YGHVYLKVEPLERGEGLKFVDKIVGGSIPREYIPAVEKGIKEAAESGVIAGYPLVDFQ
                     VTLFDGSFHEVDSSEMAFKMAAIEAMRAAQTAADTFLLEPIMKVEVITPEEFMGDVIG
                     NLSSKRGKIESTEQKGNARIILGKVPLAEMFGYATELRGMTQGRASFVMEPSHYEEVP
                     SNIAQTLAKDSIRH"
BASE COUNT         3010 a         2482 c         1486 g         4295 t
ORIGIN      
        1 ttagcttttt cggaatatgt aaaatcttta aaaactactt tctttttcaa ctcacctttt
       61 gagagtatct taacaccacc tgatggaata tcttttaata ctttcttttc tactaaagat
      121 attggagtaa tctcttcacc gtccttaaat ttgttgaaaa tatcaagact cacacccaat
      181 gctttgcttt ttgttatata aggtctctta aaaccaccaa ttcttggaag tttcttatac
      241 aaaggtgatt gacctccttc aaaccctaaa ttaggttttg cgccttttct ggctttttgt
      301 cctttttgac ctcttccaac agtatgtcca cctttacccg aacctctacc tcttccaact
      361 ctcttggatt ttttgtcatt atttaatttt ttaatattaa aaagttccat attatttttt
      421 aagttcattt aatgctttta ttgcgcatct gacgtttaaa agcttattgt tagaacccaa
      481 cacctttgaa gaaatgtctt tgacacctgc catttccata actattctga ctgaaccacc
      541 ggctataact cccgtacctt cgggggcggg tttgagaagg actttggaag caccaacttt
      601 agccgtaacc tcgtgagcga tggtcttccc tgatattttt acatcataca tcttcttttt
      661 agctgaattt atagcctttc taattgcgtc agctacgttt tgggctttgc cgaaaccata
      721 accaacctta ccatttttat tacctactac cacaagaaca gaaaaaccaa tggcgcttcc
      781 acctttgatt ttctttgaga ctcttttgat ttcaattatt ttttcctcaa attcacttat
      841 tttcgcagta tttgaatatt gttctttatt tctttcttcc tttttcataa tttcaaacct
      901 ccctctcttg ctccttcagc aactgctttt actcttccgt gatatctata accacctcta
      961 tcaaaaacta cactttttat acccttttcc aaagcaagtt ttgatatctc tttacctact
     1021 tcaaacgatt tctgtgtttt tgtggtacct tttgcgcttt ttaatttttt agatgaaaac
     1081 tctacgatag taccagattt ttcgtcatct ataatttgag cgtagatatc tttgttagac
     1141 ctaaatacgg acaatctcgg tgtttcaaaa gtaccgtaaa tcttatttct aattgatagt
     1201 cttctttttt ctctatttgt ttttcttgat tttacgtgat acatgtttat gcagctcctc
     1261 ctgaaccaac cttacctgct tttcctggtt ttcttctaat aacctcatca gcatatttaa
     1321 ttcccttgcc tttataaggt tctggttttc ttacttttct tatctttgca gctgttaaac
     1381 caactaaatg tttatcaaag ccagagacag tgatgtgagt attatcctca actttcaact
     1441 caacaccttc tggggattta aaatccacag gatgagaata tccaactgtt agtgatatct
     1501 ctctagggga tacagacttc actctgtaac ctacaccaac taactccaat tttttctcat
     1561 aaccctcggt tacacccttt accatatttg aaatcatagc tcgggtagta ccccacaaag
     1621 ccggggattc tttactttta cctatcatct caacattaat tttatcttct tcttttttga
     1681 caactacatc atcggaaaat ttaaaactca agttaccttt taatccttta acaataactt
     1741 cgtttcccgc tatttctacg gtaactccct gcggtattaa aataggtgct tttccaattc
     1801 tgctcatttt ttaataaacc tcacaaataa cttctccgcc taattttttc tttcttgctt
     1861 cctgggcgtc cataattccc ctggatgtag ataatatcat tactcccaaa ccttgaagaa
     1921 caggctttac ttccttgtag ccaatgtaag ctcttctccc gggtttagaa accctcttag
     1981 cttctgataa ttttattacg ttaccatctt ttgctaaagt tattttcaac atttttgctg
     2041 agctgttttc tttcttaaag gtttttactt cttccacaaa acccctatcc tttaaaacct
     2101 tagcaacgga ctcacattca gatgtaaaag gaatctccag agagtctctt ccacccatcg
     2161 aggcgttttt tattgaacta accatgtttg ataatctatc taaactcatt ttaccaactt
     2221 gcctttctta ctccgggaag ctctccttta tgagccatct ccctaaaaca ttttctacaa
     2281 actttaaatc ttctcatata acctcttggg tttccgcata tagaacatct gtgatattcc
     2341 cttgacgaga atttttgttt tctactatgt ttttgtttta aagcttcttt agccatttta
     2401 tttactttct atattagccg attccgaaaa gggaaaactt agcttcttaa gtagatacct
     2461 attctgatcg atattctttc cattaaaaac tacggtcact tgtagacttc taataccttt
     2521 agttgtattc ggatttactt caggaaatat aacatgttct ctaataccta ccgagtaatt
     2581 tccattctca tcaaacgagt cgggactaaa acctctgaaa tcccttattc ttgggaaagc
     2641 gatattgatt aacttatcca aaaacgccca cattctatca cctcttaagg taacagtata
     2701 ccctacaata tcattttgtt taattttaaa acctgcttct gattttcttg cttttctctc
     2761 aaatactttt tgtcctgcta tatcagacaa ttctgaagca aagaaatctg cagcctctct
     2821 attttctctg aaaggaccta ttcctgcatt tacgctaatc ttcttaatag aaggaacttc
     2881 catcacattg cctaatttaa attctttaag catatctttg gctatttctt tgttgtattt
     2941 ttcttttaat ctattcatat ctttttatct aatatttcac cggtttcttt agatactcta
     3001 tattttttgc cgtcaactaa tttatatcca atcttcacaa cttttttcga agaatcatca
     3061 taatacatta cgttagaaac atctacaggt ttttcgatac tgataatccc accctctttt
     3121 gaaattacac ccggtttaac atgctttttt actacattaa caccttcaac tacaatttta
     3181 tttttatctt ttaaaacttt caggattttg gatacctttc ccttatcttt tcctgaaata
     3241 acttttactt tatcgtcttt ttttaatctc atattataaa acctccttag ctaaagagac
     3301 tatctttgaa taacctctat cccttatctc tcttgccacg ggaccaaaaa ctcttgtgcc
     3361 tatcatattt ttatctttat ctactacaat agccgcgtta tcatcgaatc ttatatagct
     3421 accatctttt cttctgactt cttttttagt tctgattaca atggcttttg ccatactatg
     3481 atcttttaga gtacctgtag aggaagcgcc ttttactaca acagtgacgt aatctcccaa
     3541 tctggcagat cgtttatttg atccgggtat gccaacaatt ttaagcatta tcgctcctga
     3601 attgtcagct ggttttaaat atgatccaat aggtaacatt ttatttttgc tcctctttag
     3661 aaattttact taaaacttta aacgccacat ccttactaaa tggcttgcat tcctcaatta
     3721 ttacctcatc gttaaccatt actccgatat catctctggc tttaagttta gtagtatttt
     3781 ttatactctt gttgtactta ggatgttttt taggagattc cacagctaca acaactgttt
     3841 tctgcatttt attagatata acttttcctt taaatgtttt aacgctcatt tttgtacctc
     3901 ttttaatttt tcatttatta ttgtttgaat tcttgcgata tcttttctaa attgagaagc
     3961 ttttttaata tttttttcct tcttttgcat aacatcagat attgttttat gaaagtcctt
     4021 tttaactttt aagagctcct tttttagatc atctaattct ttttttctaa attcttttat
     4081 gtctatcatt ggtcatccct tcttacaaac tttgttttta tcggcaactt tgatgctgcc
     4141 ttataaaaag cttttttagc tacatcctct gttacacctg ccatctcaaa tataactttt
     4201 ccaggcttaa ccattgcagc ataatggtca gtgttggatt taccgcttcc cattcttgtt
     4261 tcatttgcct tttttgcaac tggtttatca ggaaacgctc taatccaaag ctttccaccc
     4321 cttttggttt cgtgtgtaat agcttttctt gctgattcca gctgcctgta agataacaag
     4381 catctatcta caaccttcag accataatct ccaaaggata tagacgatcc tctgacagaa
     4441 atacccttat tagtacctct ttgctgtctt ctgaatttta cttttcttgg catcatcaac
     4501 atattatttc tcctatttaa gctctatctc tcctttgtat atccaaactt ttattcctat
     4561 ggttccaaaa agcatttgac aatgtatttg agcataatca atatcagctc ttaaagtttg
     4621 agccggaacc gaacctaatt tataagtctc tgatcttgag atggtgttac taccgcttaa
     4681 aactcctgct aattttattt ttataccttt tgcaccttta tcaattgctg aacttaatgc
     4741 aaaattaaca actctcctgt aaggaactct tctttttaac tgcctacaga tgtaatctcc
     4801 aaccaactgg gcctcgattt cagaagattt aacaccttca gcggtaatct ttaccttacc
     4861 tttggttatc ttcttaatct ccttttcaat ctcctcaaca ccggtaccac ctcttccaat
     4921 aaccacaccg ggttttgaca cttttatcaa aatactaact tcgttttcag ttctttctat
     4981 ctcaacattt ttcaaacctg ctgtacttaa cttctcattt agaaaatctc tgattttttt
     5041 atcttcaatc aataaattgg cataatccct cttagatgtg taccatcttg atgaccagtt
     5101 ttttgatatt cctactctgt atccaacagg atttattttc tgacccatta tttttgatcc
     5161 ctttcatcta aacttacata aatatgacta tatcttttta aaatagggtc aactcttcct
     5221 tttgcaccag gactcattct ctttaaagaa ggccctccgc ttacccatat tcggttaata
     5281 aatagtttat ctttgttcat tttactatta tttacggcat tagcctcggc agattttacc
     5341 acttttaaaa caatttttgc agacttactt ctgtcaaaag ataaaatctt ttttgcttca
     5401 aaaacacctc tacctctaac taaatctagt acaatagcta ccttcttagg cgatactctt
     5461 gctcccatgt gttttgctgt tatcttcaca tcgtacatat tattcagcaa ttcctttctt
     5521 tgatgagtgt gctctaaagg ttctcgtagg agcaaattca ccaagcttgt gaccaaccat
     5581 tgattcagtg acaaagaaag agatatgttt cttaccgtta tgaacttcaa aagttaaacc
     5641 aaccatatta ggaactatgg ttgaatttct agcccatgtt tttattggtt ttttatcacc
     5701 tgataaagcc ttttcgactt ttttcattaa tttttcgtct gtataaattc ctttttttag
     5761 tgatctagac atatttattt ctttttgccc tttcttctac taactatagt gtaatcagtt
     5821 ctaactcttt ttctagtctt aactcccttt gttttccatc cccaaggtga cagcggtgct
     5881 actataccaa tacctgaagt actatatttt cctgcatgag gatgatctcg atgcgggtca
     5941 cccataacaa ttcctcttac gtgaggtctt cttcctaaat atctcattct accagctttg
     6001 cctaaatcaa catttctttt atctatgtta ccaataactc ctatagtagc agtacaaaca
     6061 tcaggaattc tcttaacctc accgctggga agttttacat taacataatt gccttctttt
     6121 gcaataatct gagcctgatt tccagctgat ctgcacaaga ctgcaccagc gcccggatta
     6181 atctctatat tatgaattga agttcctaaa ggtattttgg acaaaggaac gttgttacct
     6241 aaagaaattt ctgcattttc tcctgatatt attgtagatc cgacctccaa accttcggga
     6301 gcgacgatgt atcttttttc cccgtcagca taagctaaca aagctatgtt gggtcctcta
     6361 ttaggatcat cctgaatact tataactttt gcaggaatat taaatttatc tcttttaaaa
     6421 tcaacaaatc tgtagagttt tttggctcct ctttgtttat gtctgcttgt taccttacct
     6481 ctggatctac ctgaattgga tgatcttcct tttgtaagtg acttttcagg acgtacttta
     6541 tctacacctt ttaccaaggt ttttctggtt cttaatcctt cacttgttgg tttatatgac
     6601 tttatcattt aatttattta tccttaggaa ataaatctat actttgtcct tcttttaatt
     6661 ttacaactgc ctttttccac ttagcaggcg ttgtaaataa tctggttctt cctattcttg
     6721 aggatttacc aggtaatatc gcagtcttaa cttctgtaac atcaactccg tacaaatctt
     6781 ttatctcact tcttatagca ccctttgtgg cctttacaga aacttcaaac acatatcttc
     6841 caaaagaaga gtgatcatat gatttttcag taattattgg ttttattatt gatttactta
     6901 atctcattta gaaaacctct cttgaaaatc taaaactgca tttttcaaaa acacgatatt
     6961 tttagttctc attacatcgt aagcacctaa acttttaaca tttactaaat ttacttcagg
     7021 gatatttctt gcactcctta tcatattttc gttttcacct aaccaaacta aagaaagact
     7081 acctgacact tttatctttt ttaaagattg tgaaaactct ttagtatttg gttttttaat
     7141 atctactccg tcaataatga ttacttttcc ttctttcatt cttgtagaaa gagctgaact
     7201 taaagcaaca cctttcattt ttttgcttag ttgagatttg tatgattttg gcttaggacc
     7261 atgtgctact ccaccaccta cccaaatagg acttctaata gaaccttgtc ttgatcttcc
     7321 cgaacctttt tgagctcttg gttttaaacc tccgcctgaa acttctgctc tggtttttgt
     7381 agaagaagta ccttgtctct ggttattttg atatacccta atatattgag ctaaaactgt
     7441 cggattataa tctgacccaa aaatctcatc cctgagatct atttgctcta gcttttcacc
     7501 ttttaaatta ataacatcta ctttcatatt ttaaaaatta tttcttactt tctacaaatt
     7561 taatttcaat tttagaattt ctgggacctg gaaccggccc tgaaaccatt acctgtttta
     7621 attcaggcat tacttttacc agttttaaac cttttattgt tatcctctga ttaccggctc
     7681 ttccggccat tttcttgcct tttaaaactc ttccgggtgt ttgagacccg attgaaccag
     7741 gcgaccttcc tttaatacct tgacctcttg tagcgggccc gccgtgaaaa ccccattttt
     7801 tcataactcc tgcgaaacct ttaccttttg atttaccaac cacaacaata tctttatttt
     7861 caaagtctac actgacatcc gactcacagg aaataatagt tacaggaaca actactccat
     7921 tatctccgaa tagttgagtc atatttaatt tttttccaat tattttttcc attttttgta
     7981 tcaaaattaa aaaaaattta gcgcccgaag ggcgctattc gcagttaccc tttttaaaaa
     8041 aaccgttttt tatgttcttt tatttatcat taattttaaa ctaactgacc aatatatcaa
     8101 aaacaagata ggatactaca tcttaatcga aatgccaacc cctgcaggta aacttagttt
     8161 tgacaaagtc tcaacagtgg agggagtcgg gttaagaaca tctataactc ttttatgagt
     8221 tctaagttca aattgctctc tagatctttt atcaatgtga ggacctctaa ttacagtaaa
     8281 tttttcaatt ttagtaggca aaggtacagg acctacgatt tgtgcgccgg ttctaattgc
     8341 tgtatcaaca atctttgaac agctcaagtc tatgatccta ctgtcgtaag attttagttt
     8401 tattcttatc tttgtatctt tttgtgtttt accttttgcc atcttagata aagggggccc
     8461 tgcctacggc agggccctct aaaagaaatt atctttatgc gataacttta cttactactc
     8521 cgtgacctac ggtgctacca ccttctctga tagcaaatct cattccttct tccaatgcta
     8581 cgggagtaat taacttaact gtcattttta catgatctcc tggaagtacc atctcgactc
     8641 cttcaggaag ttttgtttct cctgtaacgt cagtagttct aacataaaat tgtggtctgt
     8701 atcctgaaaa gaatggttta tgtctaccac cttcttcttt ggacaaaacg taaacctctg
     8761 catcaaattc tgtatgaggt ttgactgacc ctggtttagc aagaacctga cctctttcaa
     8821 cgtcggttct ttcaattcct cttaccaaaa ctccaacgtt atctccagcc tgaccttctt
     8881 tcattgattt tttgaacatt tcaattccgg ttacaatggt ctttttggta tctcttattc
     8941 cgactatctc tatttcctcg tttagctcta cttttcctct ttcaattctt cctgtaacaa
     9001 ctgttcctct tcccgaaatt gtgaaaacat cttctacagg catcaagaat ggtttatcta
     9061 attctcttga aggagtagga atgaattcat caacagctgc cattaattct tcaattttct
     9121 taacgtattc gggatctcct tccaaagctt ttaacgcgga tccttttatt ataggagtgg
     9181 tatctcctgg gaatccgtat ttatttaata attctctaat ttcaagctca actaagtcca
     9241 aaatttcttt atcctggacc atatcacatt tgttcataaa aactaccatt gcaggaacgt
     9301 taacctgtct ggccaacaaa atatgctctc ttgtttgagg cataggtcca tcaggagctg
     9361 aaactacaag aattgcacca tccatttgcg cagctcctgt aatcatgttc ttgatgtagt
     9421 ctttgtgacc cggagcatct atatgtgcgt agtgtctctt ttcggtctca tactctacgt
     9481 gagtgatgtt aattgttata cctctttctt tttcttcagg ggcattatca attgtgttaa
     9541 attccctgta agaagccata cctttatcgg caagtacttt tgtaatagcc gatgttaagg
     9601 ttgtcttacc atggtcaacg tgacctatgg taccgatatt taaatgttct ttatttctta
     9661 cgaaatcagt cattttaaat ttcctttcga taaataaatt actttttatt tttagatcac
     9721 attattctgt acgcttgcga atgttcttct cactataaag aaccctcgct ttcgtccgga
     9781 acaacgtcat ctaaagctca aaacaattac ataagcaagt gatagttaat caaattaaat
     9841 caaaaaaatc aatactttta gcgctgttac aaaaaatcag tgcctaatac tatcctttgc
     9901 caaggtctgc gctatgtttg acggaacctc ttcatagtgg ctgggctcca taacaaaaga
     9961 cgctctaccc tgagtcatac ctcttaattc tgttgcgtat ccaaacatct cggctaaagg
    10021 tacttttcct aaaattattc ttgcattacc tttttgttca gtgctttcaa tcttacctct
    10081 tttagaagat aagtttccga taacatcacc cataaactct tccggagtaa ttacttcaac
    10141 cttcatgata ggttctaata agaatgtgtc cgcagctgtt tgagcagctc tcatagcttc
    10201 tattgcagcc atcttgaatg ccatttccga agagtctact tcatgaaaag atccatcaaa
    10261 aagtgtaacc tgaaaatcga ccaaaggata acctgcaata actccgcttt cagctgcttc
    10321 cttgatacct ttttcaacag ccggaatata ttccctagga attgaacctc cgacaatttt
    10381 atctacaaac ttcaagccct cacccctttc caaaggttct acttttaggt atacatggcc
    10441 gtactgacct ctaccacctg attgcctaat atacttacct tcctgctcaa caacttttct
    10501 aatagtctct ctgtacgcaa cttggggttt tcctgtgttg atttcgagat taaattctct
    10561 tctcattcta tcgacaataa tatctaagtg aagctcgccc attccgtaca gtagagtctg
    10621 tccggtttca atatttgttt taatttgtaa agtaggatcc tcctcaagga atttcttaat
    10681 tacttgagac atcttatctc tatctgcttt ggttttaggt tcaataacta ttccgataac
    10741 cggctctgcg aaactaatct tatctaataa aattttatgt tcttcgttgc agagtgtgtc
    10801 acctgtggta gcttcaagac caactccggc tgcaatctca cctgccaaaa cttcttttat
    10861 ttcttctctg tgatttgcgt gcattaataa aattcttcca agtctttcct ttttacctgt
    10921 tgttgaattg taaacataag aaccggcggt tatttttcct gaatacactc taatataagt
    10981 taacctacca acgtaagggt ctgtttggat cttgaatgcc agtgctgcca aaggtccgtc
    11041 aatatctgaa ctaagggaga cttcctcacc tgtatttgaa tttatccctt ttatatcagg
    11101 ggtatcttta ggtgagggaa gatattcaat aacaccatcc agaacttttt gaactccttt
    11161 gtttgataga gaagcaccac aaaagatcgg gtacaattcc gctttaactg tcatttttct
    11221 aatagctttt ttaatttctt caatagtgag ctccccgccg ttcaaatatt ttt
//
LOCUS       DAOTFA010000009        10032 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate AMR_MDS_3115
            NODE_4741_length_10032_cov_7.857272, whole genome shotgun sequence.
ACCESSION   DAOTFA010000009 DAOTFA010000000
VERSION     DAOTFA010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868139
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (wastewater metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 10032)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10032)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:36:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 536
            CDSs (total)                      :: 521
            Genes (coding)                    :: 520
            CDSs (with protein)               :: 520
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10032
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4741_length_10032_cov_7.857272"
                     /isolate="AMR_MDS_3115"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..440)
                     /gene="recO"
                     /locus_tag="P5014_00740"
     CDS             complement(<1..440)
                     /gene="recO"
                     /locus_tag="P5014_00740"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HRY56672.1"
                     /translation="MKKYNVRAVVLKSLKYRDSDKIFTLLSKEKGKISAIGRGVRKIS
                     SRRNGNLDTLNLISANIHEDGAGYKGIEEVKTIESFKDLKKDFGKSIKAYYLIELVHK
                     SIEEGESVPEVFNLLIKCLKALDKNNYSGDLYTSYFEINFMKILG"
     gene            complement(448..2403)
                     /locus_tag="P5014_00745"
     CDS             complement(448..2403)
                     /locus_tag="P5014_00745"
                     /inference="COORDINATES: protein motif:HMM:NF024594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4012 domain-containing protein"
                     /protein_id="HRY56673.1"
                     /translation="MANSKFWSASSSGSTIPTISKTTKQSSKTKKKIGIGLLIFFLLV
                     AIVLGLGYYFVGRPALAIYSKANTLKSDISLVGEAFQNRDLVQLNEVLDKTEQDINDL
                     RKDKDEKFSWAKNLKLFKVNEYYSDFDKFSNAGIYAVDAVREMSRVITPFADAAGLKV
                     SPDQELPKAEGLMEAFQGWVSVMPQVANDMDGVIERIGKVGDELNSVNVGKYPEKIGK
                     TEIRSNVEFIKNSLSKADDYAPDIKKALQIVPRLLAVGTPTKRYMIIMQNDKEIRPTG
                     GFMTNYATFKISNGLLDSDFTSKDMYSVDLTLDQIDAYTDFPDPPAPYGSLLKVERWY
                     ARDTNYSPDFVTSMDEFLSYYNWAGRISPFEIKPVDGIIAIDTNVISELLEVTGPVTV
                     NGTTYTQDNVVLELEKVASLALAEQANRKRVLGDLMQGMLINVFESDSNLWPKLIDKG
                     VNLAIRKHISIYVFDAEAQALLDSYGVGGRIKENVDGDYSMVVSTNLGGDKTNWFTNK
                     QVEHALEKEGDKWIRTVKIKYQYTEPNGDYSAFVKRFRDWVRVYAPIGSELISVQGSE
                     AEAYNLSDQERNKIWFSGYLELGPGESKEITFKYYVPSEVIGEKTYKLDIQKQSGIDK
                     EKHTVTYGNQTKEVELIKDTFVEISKQ"
     gene            complement(2403..3044)
                     /locus_tag="P5014_00750"
     CDS             complement(2403..3044)
                     /locus_tag="P5014_00750"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014461199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HRY56674.1"
                     /translation="MIYRNYIKRLVDIIGSLVGIVLFLPLMIGVSIYIKIVSPTGPVL
                     ADIPNRVGKDKKPFKFFKFRSMYPNAHEMLKKDPELYAKYVKNNYKLHADEDPRLIKG
                     GKFLRKSSLDEFPQFFNVLKGDMSLVGYRAYYFDEVEEQTKRFPESKEYMEKVMTVKP
                     GMTGLWQVSGRSDVGFVDRVKLDAKYAENVSLMYDLKIILKTPYVVLLGKGAI"
     gene            complement(3069..4184)
                     /locus_tag="P5014_00755"
     CDS             complement(3069..4184)
                     /locus_tag="P5014_00755"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRY56675.1"
                     /translation="MKLAIVCDDLIQKGGAEKIVEAFSDIFPDAPIYTTIASEDWFKK
                     MESKNRIVKTSFLQKFPFSVKLNRFYASFLLHILAIESFDLSEFDVVLSSSSRFAHFA
                     LTKPSTKHISYVHSPGRMFWEPFDYFEKESFGVFSFIKKVALGFLKLPLMYIRMKDYE
                     ASQKVDMFLTNSITSQKRIKKYYGRDSEIVYPFVNIEKLKFTESADKSAKRDFYLIIT
                     RLAAWKKVDIAVKAFNDLGIELRIIGDGPDLNRLKKMAKQNIKFLGYASDSEKIENIK
                     NCRAVIVTQKEDFGIVPLESMACGKPVIAFGEGGATEIVIEDKTGEFFYEQRPESLIN
                     IINNFNPDKYITSDCIERARFFNKEKFELEIKRIINV"
     gene            4217..5344
                     /locus_tag="P5014_00760"
     CDS             4217..5344
                     /locus_tag="P5014_00760"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HRY56676.1"
                     /translation="MKIGLNGQVIQISNPAGPEVYTINLFKNLARVDSENDYTIYLDK
                     TPTEEFFNEVSGGNPRFSYKVIEGKRLWTQYHLAKELLKNPVDVFFTAIHTIPIIRSK
                     KTKFVVMIHGLEYKFSEGFKNPLKKFKIERPIRCAAINSDKIIVPSKATKDEILKRRW
                     GVKDEKIEIVYEGVSNEFYERDEIEVQKIREKYKIEDSPYLLFVSTIQPRKNLPSTIR
                     AFGQFLNENPEMKNTLLLIAGKNGWDYQESLEAPLKYGVVGNVKFLGRVPTEDLPALY
                     SGSKGYINISYEEGFGLPLLESMACGKPSVVSDIPAHREVGGSLPIYADPYNIENIKD
                     GIFKIMTSDFDKNLLLDRAAGFSWKRTAEKTLEILKETVKK"
     gene            complement(5437..6585)
                     /locus_tag="P5014_00765"
     CDS             complement(5437..6585)
                     /locus_tag="P5014_00765"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HRY56677.1"
                     /translation="MSETKKIGIDARFYGKAGPGRYTKNIIKHLEKIDNTNQYFILLK
                     KDNFDNYVPSNPNFKKVLADYPWYSWAEQTSFLFKVLSLNLDLYYVPHFNIPVLYPKK
                     IVTAIPDMIMHTFSTEKGTTLWKPYFKFKKLIYRLVFKWSVIRSFKVIVPSEEVFRDF
                     KTVYPDISDDKFVVATEGVDPDLMNIHVEDTKAVLKKYEIEKPFLLYLSSMYEHKNVP
                     RLLEAFKLLIDEYGYKGNLILIGKKDKFSEKIQELVGEMKLGDRVLMPGMKGFVTDEE
                     TVALRKEADAYVFPALKEGFSLTPLEAQYYGLPCVISDIPCHKEVYGDSVMFFDPYSV
                     EDIAKKINKVLTETDLRADLIEKGYSRIKIYNWTHTAEKTLEVFNQAL"
     gene            complement(6640..7641)
                     /locus_tag="P5014_00770"
     CDS             complement(6640..7641)
                     /locus_tag="P5014_00770"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRY56678.1"
                     /translation="MKKISFIIVNYATKGLLEKNISNLLSIWENSEVIFIDNDSPDGS
                     ADFVEEKFGKDPRVISIRNQNNGLSAGYNKGLKVATGDYLVYLGTDSFPTKDAIEGML
                     KYMDENLDVGIVTPKLFTRDGAVDIDAHRGFPTPWASITHFTYLDRLFPKSRIFNQYS
                     RKYQDFNTVHEIDACISHFMMVRPEVHKKVGGWDEDYFLFGEDIDFCYRTKQAGFKIM
                     YMGNIPVLHYKGASVGRDTSKDIDNVMNTDFDKISFKGQVFGKESADGSFTVNNNGSL
                     RDWMKKQISKESTDAMKTFYKKHFVKKYPFFLNWFVFLGIWVNEQIRFLKVFIKSKR"
     gene            7713..8930
                     /locus_tag="P5014_00775"
     CDS             7713..8930
                     /locus_tag="P5014_00775"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HRY56679.1"
                     /translation="MKKSLIRIGLVGMGNVFNALLNFTFLSMIAKTLDIESFGKYALL
                     ATLLVAVSKIIDFGTNSVYVADSISKDDETLLDTFYTLKLILLTISIPISIILLLLLK
                     LNSPNIILYFILGLIAYTINYTLNSFFQKDQKFLHLILLNTFPALIKGGFAVLIILDM
                     VVFNLDRSFMVFSLSIFSSAIMWFFLPKEFKKFRFDFSKVKIYLKKSAPAGVSQLIFE
                     GWPSISNSIAKIANSFSHVGIFAIAEKVANILQLGAISIFTILLPKNAYAKKRNEKYD
                     FKEVFVISILILVIAFFGTFISRYFVSDFFDDKFAMSVPLLALLIFSSAFTSIHNFME
                     NYFFIEEKTNSIMYINVGKLVLFLLISLILVPTYSLKGLAISNLISAMAALAATIIFI
                     SVDQKLKATEAQE"
     gene            complement(8911..>10032)
                     /locus_tag="P5014_00780"
     CDS             complement(8911..>10032)
                     /locus_tag="P5014_00780"
                     /inference="COORDINATES: protein motif:HMM:NF014425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000030 - mannosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006493 - protein O-linked glycosylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid carrier-dependent
                     glycosyltransferase"
                     /protein_id="HRY56680.1"
                     /translation="LALVTTVHFLYYSRASMLDVTTTFFITLALYLYWKANENKKIFF
                     WILSGLSTGLAVMVKGFVGLLPLLIIGIYELLLIFVFKEKLKKKKLIGYFLMFLSLLF
                     VALPWHFVMYQKFGTSFIDKYFLYHVWDRAVMAIEDKGNPFFWYLTVMKVSMRIWFIA
                     LIGAFPFTAFKSFKKDKKNLFLLVWALSIFLFFSSAKSKLVWYIIPIYPVVSIMVGAF
                     SEELLGFVMKKIKIFNNLYFKFLALSFVLIFSLFYLYLNREKAYPSDLNGAPAYLLMQ
                     KDRIFGIERTVYLDRVDTPTALFYTDGPFVLTDVNVNEPNRVPEVSDDEPLIFISKKG
                     RYSEKVPGYSKKSKIVKEFDPYYLWYVPSESGAFILELQ"
BASE COUNT         3277 a         1871 c         1446 g         3438 t
ORIGIN      
        1 ccaagtattt tcataaaatt tatttcgaaa taacttgtgt ataagtcccc tgaataatta
       61 tttttatcca aagcttttaa acatttaatt aataaattaa aaacttctgg taccgactca
      121 ccttcctcaa tggatttatg aactaactca attaaataat aagctttaat ggattttcca
      181 aaatctttct ttaagtcttt aaaagattca attgttttta cttcctcaat acctttataa
      241 ccagccccgt cttcatgaat atttgctgaa attaaattta gagtatcaag atttccgttt
      301 cttctggaac taatttttct tacacctcta cctattgcgg atatttttcc tttttcctta
      361 gagaggagag taaatatttt gtcagagtct ctatatttga gacttttcaa cactactgcc
      421 ctgacattat attttttcat acaagcctta ttgcttagat atttctacaa atgtatcttt
      481 tattaactcg acttcttttg tctgatttcc ataagttacc gtgtgttttt ccttatctat
      541 tccagattgt ttttgaatat ctaacttata agtcttctcg ccaataactt cagacgggac
      601 atagtatttg aaagttatct ccttagattc tcctggacct aattccaggt aaccactgaa
      661 ccaaatttta tttctttctt ggtctgacaa gttgtaagct tcggcttcag aaccttgtac
      721 ggagattaat tcggatccta taggagcgta cactctcacc caatctctaa atctctttac
      781 aaacgcagaa taatccccat taggctctgt atattgatat ttgatcttta cagttctaat
      841 ccatttgtca ccttcttttt ctaaagcgtg ttctacctgt ttattagtaa accagttcgt
      901 tttatctccc cctaaatttg tagaaacaac catagaatag tcaccatcaa cattttcttt
      961 tattcttcca cctacaccat agctatctaa aagtgcttgg gcttccgcat caaaaacata
     1021 aatcgaaata tgttttctaa tagcaagatt aactccttta tcaataagtt taggccataa
     1081 attactatct gattcgaaaa cgtttattaa cattccctgc attaaatcac ctaaaactct
     1141 ttttctattt gcttgttctg ccaaagccag ggaagcgact ttctccaatt ctaatacaac
     1201 gttatcttgg gtatatgtcg ttccatttac tgtaacaggg cctgtgactt ccaaaagctc
     1261 ggagattaca tttgtatcta tggcaatgat tccatctaca ggtttgattt cgaaaggact
     1321 tattctaccg gcccagttgt agtagcttaa aaattcatcc atcgaagtaa caaaatccgg
     1381 cgagtagttt gtatctctgg cataccatct ctctactttt agcaaactgc cgtatggtgc
     1441 gggtggatcc ggaaaatcag tataggcgtc tatctggtct aaggtaaggt caacgctata
     1501 catatctttt gaagtaaaat cagagtctaa caaaccgttt gatatcttaa atgttgcata
     1561 attagtcata aaacctccgg tcggtcttat ttctttatca ttttgcataa taatcatgta
     1621 tctttttgtt ggcgtgccta ctgctaagag tctaggtact atttgtaagg ctttttttat
     1681 atctggagcg taatcatcag ctttagaaag tgagtttttt ataaactcaa catttgatct
     1741 aatttcagtc ttccctattt tttcaggata tttacccacg ttaacggaat tcaactcatc
     1801 tccaactttt ccgattcttt ctataactcc gtccatatca ttcgcaactt gcggcattac
     1861 agaaacccaa ccttggaaag cctccatcaa accttcagcc tttggtaatt cctgatcagg
     1921 agatactttt aatcctgccg catcagcaaa aggagttata actctggaca tttccctaac
     1981 agcatcaact gcgtatatac cagcattaga aaatttatca aagtctgagt aatattcatt
     2041 aactttaaaa agctttaaat ttttcgccca tgaaaatttt tcatctttat cttttctcaa
     2101 atcgttaata tcttgttctg ttttatctaa tacttcgttt aactgtacga gatctcgatt
     2161 ctgaaaagcc tctccaacaa gactgatgtc ggattttaaa gtattggctt ttgaatatat
     2221 cgctaatgca ggcctaccaa caaagtagta acctaatcct aaaacaattg ccactaacaa
     2281 aaagaaaatt agtaaaccaa taccaatctt cttttttgtc ttagaggatt gctttgtagt
     2341 tttactaatt gtaggtattg tgcttccaga tgaagaagca ctccaaaatt tagaatttgc
     2401 cattatatag ctccttttcc taacaaaacc acatacggtg tttttaaaat aatttttaaa
     2461 tcatacatca aagagacatt ttctgcatac tttgcatcta atttcactct atcaacaaac
     2521 ccgacatcag atcttccgct tacctgccat aatccagtca tcccaggctt taccgtcatt
     2581 actttttcca tatattcttt tgattccgga aatctcttgg tctgttcctc tacttcatca
     2641 aaatagtaag ctcggtaacc aaccaagctc atatcacctt ttaaaacatt aaaaaattga
     2701 ggaaactcat ctaagctgga ttttcttaaa aatttgccgc ctttaattaa acggggatct
     2761 tcgtctgcgt gaagtttata gttatttttt acatattttg cgtaaagttc cgggtctttt
     2821 tttaacattt catgggcatt tggatacatg gatctaaatt taaaaaactt gaaaggtttt
     2881 ttgtctttac caaccctatt aggaatatcc gctagtactg gtcctgttgg ggaaacaatc
     2941 ttaatatata tcgaaacacc aatcataagt ggtaaaaata aaacaattcc tactagagaa
     3001 ccgattatgt cgaccagtct tttaatataa tttctataaa tcatagtcta aactaaggct
     3061 gtagaacatt atacattaat tatccttttt atttccaact caaatttctc tttattaaag
     3121 aagcgcgctc tttcaataca atctgaagta atgtatttat caggattaaa attatttata
     3181 atatttatca aagattcagg tctttgttca taaaaaaact caccagtttt atcctcaatt
     3241 acaatttctg ttgccccacc ctctccaaaa gcaataacag gcttcccgca cgccatactt
     3301 tcaagcggaa cgatcccgaa atcttctttt tgagtaacaa taacagctct gcaatttttt
     3361 atgttttcaa ttttttcaga atcacttgcg tatcctaaaa atttaatatt ttgtttagcc
     3421 atttttttta atctgtttaa atcaggcccg tctccaatta ttctcaactc tattcctaaa
     3481 tcgttaaatg ctttaacagc aatatctact tttttccaag cagctaatct ggttattatt
     3541 aaataaaaat ctcttttagc agatttatca gctgactctg tgaattttaa tttttcaata
     3601 tttacaaaag gatagactat ctctgagtct cttccgtaat attttttaat tcttttttgt
     3661 gaagttatgg aattagttaa aaacatgtca actttttgtg aagcctcgta atccttcata
     3721 cgaatataca tgagaggtag tttcaaaaac cctaaagcta cttttttaat aaaactaaaa
     3781 actccgaaac tttctttttc aaaataatca aaaggttccc aaaacatcct acccggtgag
     3841 tgcacgtaag aaatatgttt tgtacttggc ttagtaagag caaaatgagc aaatcttgaa
     3901 gatgaagata aaacaacatc aaactctgat aaatcaaagc tctcgattgc taagatatga
     3961 agtaaaaacg atgcatagaa tctatttaat ttaactgaaa atggaaattt ctggagaaaa
     4021 gatgttttaa cgatacgatt tttactttcc atttttttaa accaatcttc agaagcaatt
     4081 gttgtgtaaa taggcgcatc aggaaaaata tcagagaacg cttcaacaat tttttcagcc
     4141 ccgccttttt gaattaaatc atcacatact atagctaatt tcattattta tattatacct
     4201 atataattaa attaagatga aaataggatt gaacggacaa gttattcaaa tatccaatcc
     4261 cgctggaccc gaagtttata caataaacct ttttaaaaac ttggcccgtg ttgacagtga
     4321 gaatgattat acgatatatc ttgataaaac tcctacagaa gaatttttta atgaggtatc
     4381 cgggggaaat ccaagatttt cttacaaagt aattgaaggt aaaagacttt ggacacagta
     4441 tcacttagca aaggagttat taaaaaatcc ggttgatgtt ttttttactg caattcacac
     4501 gataccgata ataagaagta aaaagactaa atttgtagtt atgattcatg gacttgaata
     4561 taaattttcg gaaggtttta aaaaccctct gaaaaaattt aaaatcgaaa gaccgataag
     4621 gtgtgctgca ataaattcgg ataaaataat tgttccaagt aaggctacta aagacgagat
     4681 tttaaaaaga agatgggggg ttaaagatga aaaaattgaa atagtgtatg aaggagtttc
     4741 aaatgaattt tatgaaagag atgaaattga ggttcaaaaa ataagagaaa aatacaagat
     4801 tgaagattcg ccctatcttt tgtttgtctc aactattcaa ccaagaaaaa atctaccttc
     4861 aacaattaga gcttttggac agtttttaaa tgaaaatcct gaaatgaaaa atacgttact
     4921 attgattgca ggtaaaaatg gatgggatta ccaagaatct ctggaagcac cactgaagta
     4981 cggggtggtg ggtaatgtta agtttttggg aagagtacca accgaagatt tgccggcttt
     5041 atattcggga tcaaaagggt atataaatat ttcttacgaa gaaggttttg gattaccttt
     5101 gttggaatca atggcctgtg gaaaaccaag tgtagtttcg gacatacctg ctcatagaga
     5161 agttggggga agcttaccta tctacgctga cccctacaat attgaaaaca tcaaagatgg
     5221 gatttttaaa attatgactt cagattttga taaaaatctt ctactggaca gagccgcagg
     5281 tttttcatgg aaaagaacgg cggaaaaaac tttggaaatt ctaaaagaaa cggttaaaaa
     5341 gtaaaaatat tcttagctat atgaatagct gtaaagggta attattgctc tgttcaactc
     5401 taaattatag tcttaaatct ccccatcacg agaaattcac aaagcctgat taaatacttc
     5461 caaagttttt tccgcggtgt gagtccaatt ataaatttta attcttgagt aacccttttc
     5521 aatcaaatct gcccgtaaat ctgtctcagt taaaactttg tttatttttt tagcaatatc
     5581 ttcaacactg taagggtcaa aaaacataac cgaatcacca tacacttcct tgtgacaagg
     5641 aatatcggag attacacacg gcaacccgta atattgtgcc tccagaggag ttaaactaaa
     5701 accttctttt aatgcaggaa aaacataagc atcggcttcc tttctcaaag caactgtctc
     5761 ctcatccgta acaaatcctt tcatacccgg catcaaaacc cgatccccaa gtttcatctc
     5821 ccccaccaac tcctgaattt tttcagagaa tttatctttt ttacctatca aaattaaatt
     5881 tcctttataa ccatattcat ctatcaaaag tttaaaagcc tccaacaaac ggggaacatt
     5941 tttatgttca tacatactgc ttaaatacaa aagaaatggt ttttctattt catatttttt
     6001 aagaaccgct tttgtatcct ctacatggat attcattaaa tcgggatcca ccccttcagt
     6061 cgctacaaca aatttatcgt ccgaaatatc cgggtaaaca gttttaaaat ctctaaaaac
     6121 ttcttctgat ggaactataa ctttaaagga tctaataacc gaccacttga aaactaatct
     6181 atatatgagc tttttgaatt taaaataagg cttccacagg gtcgttcctt tttcagtgct
     6241 gaaggtatgc ataatcatgt ctggaatcgc ggtcacaatt ttttttggat ataaaaccgg
     6301 aatattaaaa tggggaacgt aatagagatc taaatttaaa cttaaaactt taaaaagaaa
     6361 cgaggtctgc tctgcccacg aataccacgg atagtccgca agaacttttt taaagttcgg
     6421 gtttgaaggg acgtagttat caaaattatc tttttttaac aaaatgaagt actgatttgt
     6481 gttatcaatt ttttcaagat gtttaattat gtttttggta tatctaccag gacctgcttt
     6541 tccataaaat cttgcatcaa ttccaatttt ttttgtttct gacatttttt tatctttctt
     6601 tataaatatt ttttgccttc ccgacaaatt caaactaact cacctcttac ttttaataaa
     6661 aacttttaag aatctaatct gttcattaac ccaaattccc aaaaaaacaa accagtttaa
     6721 aaagaacggg tattttttca caaagtgctt tttatagaaa gttttcatgg catctgtaga
     6781 ttctttagaa atttgtttct tcatccaatc tcttagactt ccgttattat ttactgtaaa
     6841 actaccatca gcactctcct tcccaaagac ctgaccttta aaactgattt tatcgaaatc
     6901 tgtgttcatg acattatcaa tatcttttga agtatctctt ccaacggaag ctcccttgta
     6961 atgaagtaca ggtatattac ccatatacat tattttgaaa cctgcttgtt ttgttctata
     7021 acaaaaatcg atatcttccc cgaataaaaa gtagtcttca tcccaccccc caactttttt
     7081 atgcacctcc ggtcttacca tcataaaatg agagatacaa gcatctattt cgtgaacggt
     7141 attaaaatcc tgatattttc ttgagtattg gttaaatatt cttgatttgg gaaacagtct
     7201 atccaaatag gtaaaatgag ttattgatgc ccaaggtgta ggaaaaccac gatgagcgtc
     7261 tatatcaact gctccgtcac gagtaaataa tttgggagtt actattccaa catccagatt
     7321 ttcatccata tatttcaaca taccttcaat tgcgtctttt gtgggaaatg agtctgtgcc
     7381 taaatagacc aagtagtctc ccgtggccac ttttaatcct ttattataac cggctgacaa
     7441 tccattattt tgatttctaa ttgaaatcac acgagggtct tttccaaact tctcctcaac
     7501 gaaatcagcc gaaccgtccg gtgaatcatt atcaataaat attacttcgg agttttccca
     7561 gatacttaat aagttggata tatttttttc cagtaatcct tttgtagcgt agttaacaat
     7621 aataaaagag atttttttca tattcatcct tgatgttact tcaccattat aacaaccagc
     7681 tataatagct tcaatttggt aacatataca taatgaaaaa atctttaata aggataggat
     7741 tggtaggcat gggaaatgtc tttaatgcac ttctcaattt cacattcctc tccatgatag
     7801 ccaaaacact agatatagag tcgttcggta agtatgcact tttggcgaca cttcttgtgg
     7861 cagtttctaa aattatagat tttgggacta actcggtata cgtggcagat tctatttcca
     7921 aagacgatga aacacttctg gatacttttt atacattaaa attaattcta cttacgatat
     7981 ccatacctat ttcaattatc cttctattac tactcaagtt aaattctcca aacataattt
     8041 tgtattttat cttaggtctt attgcttaca ccataaatta cacgctgaat tcattttttc
     8101 aaaaagatca gaagtttctt catttaattt tacttaatac atttccagct ttaattaaag
     8161 gtggttttgc agttcttata attctagata tggtggtatt taatctcgat agatctttta
     8221 tggtattttc gttatcaatt ttttcaagcg ctatcatgtg gttttttctt cccaaagaat
     8281 ttaaaaagtt tagatttgat ttttcaaaag ttaaaatata tttgaaaaaa tccgccccgg
     8341 ccggagtatc tcagttaatc tttgagggtt ggccttcgat atctaattca attgcaaaaa
     8401 tagcaaatag cttttctcat gtgggaatat tcgccattgc agaaaaagtt gctaacattt
     8461 tacagcttgg agctatttcg atcttcacta ttctcctgcc aaaaaacgct tacgccaaaa
     8521 aacgtaatga gaaatatgat tttaaggagg ttttcgtaat ttcaatattg atattagtta
     8581 tcgcgttttt tggcaccttt atctcgagat acttcgtatc tgactttttt gatgataagt
     8641 ttgctatgtc agttccactt ttggctttat taatattttc aagcgcattt acatcaattc
     8701 acaattttat ggaaaactat ttttttattg aagaaaaaac aaattcaatt atgtacatta
     8761 acgttggaaa actcgttttg tttttactaa tctcattgat attagttccc acttactcgc
     8821 taaagggatt ggctatatct aatttaatct cggcgatggc cgctttagca gcaactatta
     8881 tatttatctc ggtagaccag aaattaaaag ctactgaagc tcaagaataa aagctcctga
     8941 ttcagaaggt acgtaccaaa ggtagtaagg atcaaactct tttacaatct tcgatttttt
     9001 agaatacccg ggtacttttt cggagtatct tcctttttta ctaataaata ttaacggctc
     9061 atcatccgat acttcgggta ctctattagg ttcgttaaca tttacgtcag tcaaaacaaa
     9121 cggaccatcg gtatagaata gtgcagtagg ggtatccact ctatcaaggt atacagttct
     9181 ttctatacca aaaattctat ctttctgcat tagtagatac gcaggagctc cgtttaagtc
     9241 agaaggatag gctttttctc tattcaggta taggtaaaaa agagaaaata ttaaaacaaa
     9301 agacaaagca agaaatttaa agtaaagatt attaaaaatc ttaatttttt tcatcacaaa
     9361 acctaaaagt tcttctgaaa aagcccccac cataatactt accaccggat atattgggat
     9421 gatataccaa accagttttg attttgcaga cgagaaaaac aggaatatgc taagtgccca
     9481 caccaaaaga aataagttct tcttgtcctt tttaaatgac ttaaaagccg taaagggaaa
     9541 ggctcctatt aaagcaataa accaaatcct catactaacc ttcataacgg tcaaatacca
     9601 aaagaatgga ttgcccttat cttcaatcgc cattaccgct ctgtcccaaa catgatacaa
     9661 aaaatattta tcaataaatg acgttccgaa cttttgatac attacaaaat gccatggaag
     9721 agcaacaaat aataaagata aaaacatcaa aaagtagccg attaatttct tcttctttaa
     9781 cttttcctta aaaacaaaaa tcaacagaag ctcatatatt ccgataatta ataaaggaag
     9841 gagccctaca aaacctttta ccattacggc aagacccgtt gataaacccg aaagtatcca
     9901 aaagaatatt tttttgttct catttgcttt ccaataaaga taaagggcta aagtaataaa
     9961 aaatgtagtt gtaacatcaa gcattgaagc tcttgaataa taaagaaagt gaacggttgt
    10021 taccaacgcc aa
//
LOCUS       DAOTFA010000010         9903 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate AMR_MDS_3115
            NODE_4830_length_9903_cov_8.273964, whole genome shotgun sequence.
ACCESSION   DAOTFA010000010 DAOTFA010000000
VERSION     DAOTFA010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868139
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (wastewater metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9903)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9903)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:36:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 536
            CDSs (total)                      :: 521
            Genes (coding)                    :: 520
            CDSs (with protein)               :: 520
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9903
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4830_length_9903_cov_8.273964"
                     /isolate="AMR_MDS_3115"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(12..2261)
                     /gene="topA"
                     /locus_tag="P5014_00785"
     CDS             complement(12..2261)
                     /gene="topA"
                     /locus_tag="P5014_00785"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HRY56681.1"
                     /translation="MKLVIVESPTKAKTLSTILPKKDYIIEASMGHIRDLPKSGLGVD
                     VEKDFEPEYIVPPKAKKTLNTLKQKSKDIDEIILATDPDREGEAIAWHLEYLLQPTKK
                     KLKTKKKEIEGEDTIDNSTTGEKVKFGRVVFHELTESAINEAFKNLGQVDMNLVNAQQ
                     ARRILDRLVGYKLSPLLWKKVMYGLSAGRVQSVAVRLIVERERERQAFKSEEYWTMAG
                     IFQDVGSQDKFKATLIEKAGKKISISTKEEAQGIEKELKDDTFIITSVKKTERSSRPY
                     PPLSTSMLQQTMSNLFGFTAKRTMSAAQSLFEQGYITYHRTDSLNLSSAFVSTVRNYI
                     QGTFGSEYLPEKPTFYKTKSKNAQEAHEAIRPTNLSLVPGKSNKKFKPDESKVYSVIW
                     KRAVECQVSAAVYDQTTVQIDSSKQYTFKSVGSQIKFDGWLVVGKYLGMSGNAENNGG
                     SEEGVEEMNSIPDVSENDRVDLKELNSDQHFTQPPARYSDATLIKKMEELGVGRPSTY
                     APTISTIQARGYVEKDGRYFIPKDVAYVVNDLLVSHFPDIVDYQFTAEMEESLDEIAS
                     GEKEWVPLIKNFYIPFEKELAEKETSLSKADVTNLGESDEKCPECGKTLVFKLGKYGK
                     FLSCSGYPDCKYAKPLAGEVGSEETGESVDHGKCPNCEDGVFVLKQGRFGKFLACSNY
                     PKCKTTKNYQDKIGMKCPECGEGDIVVKKAKKKVFYGCSRYPDCKWSSWTNPMPKEKK
                     KRESLSSLI"
     gene            2705..3250
                     /gene="def"
                     /locus_tag="P5014_00790"
     CDS             2705..3250
                     /gene="def"
                     /locus_tag="P5014_00790"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: protein motif:HMM:NF001159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HRY56682.1"
                     /translation="MSVKKIVTVPNPILNKKTQKIGEITDDIKNTARDLIDTVKVAED
                     PEGAGLAATQIGVSKRMCVVRNFFEDPLNPNEIKSNDIVLINPKITNYSDEKDLDWEG
                     CLSVPDKYGKVERALKIKVAAKDIDGNIIKIKAEGFFARTIQHEVDHLDGVLFTSKIE
                     GETLSQEEFDQMIEDQNNEQG"
     gene            3237..4193
                     /gene="fmt"
                     /locus_tag="P5014_00795"
     CDS             3237..4193
                     /gene="fmt"
                     /locus_tag="P5014_00795"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HRY56683.1"
                     /translation="MNKVDKPIKIVFFGTSDRSLPILNSLKENFNLVLCVTKSDTVVG
                     RKKSIRETEVKKWAEQNKVELITVGSLKGTDLERTLSIIEKHKPNLGVVADFSFMIPK
                     SIIDSFNGNIINVHFSLLPKYRGASPVQHAILNGDELTGVTFYLLNEKMDEGDILNQY
                     EYKIDSKITSGELYSVLFKLSAEKLPQTIVNFLKGDVKPMQQDHSKSSYTISKTNPKH
                     TFIFKEDSLINWSDGPNKIHRAVRAFNPWPIAWTYLEDLEKTDVISEKIKLKDNVNKK
                     LKVKIFTSSIENGKLKINQLQLEGKNKADWVSFLNGYCEKSK"
     gene            complement(4201..4452)
                     /locus_tag="P5014_00800"
     CDS             complement(4201..4452)
                     /locus_tag="P5014_00800"
                     /inference="COORDINATES: protein motif:HMM:NF013026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L28 family ribosomal protein"
                     /protein_id="HRY56684.1"
                     /translation="MSKQCQICGKTYQKGNLVPRGIGNRVTRRTISRNSINLRVKRFL
                     IDGRSVKLRLCSSCLKRIKKDQADIDKLIKESEGTENKE"
     gene            5014..5088
                     /locus_tag="P5014_00805"
     tRNA            5014..5088
                     /locus_tag="P5014_00805"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5046..5048,aa:Thr,seq:tgt)
     gene            5090..5175
                     /locus_tag="P5014_00810"
     tRNA            5090..5175
                     /locus_tag="P5014_00810"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5124..5126,aa:Tyr,seq:gta)
     gene            5805..6113
                     /locus_tag="P5014_00815"
     CDS             5805..6113
                     /locus_tag="P5014_00815"
                     /inference="COORDINATES: protein motif:HMM:NF013869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MscL family protein"
                     /protein_id="HRY56685.1"
                     /translation="MKGFIDFIRKQGVVGLAVGFILGGAVSKVVASIVADIINPILGL
                     ILGAAGGLQQMVIKIGSAQLLIGNFIATLIDFIVIALVVYYGVKALKLDRLDKKEDKK
                     "
     gene            6147..6221
                     /locus_tag="P5014_00820"
     tRNA            6147..6221
                     /locus_tag="P5014_00820"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6179..6181,aa:Thr,seq:ggt)
     assembly_gap    6989..7088
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7089..7883)
                     /locus_tag="P5014_00825"
     CDS             complement(<7089..7883)
                     /locus_tag="P5014_00825"
                     /inference="COORDINATES: protein motif:HMM:NF014881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HRY56686.1"
                     /translation="MKIFDSKIQSEILEKNISEALDKGNGAGKVLSIVQVGNNESSNK
                     FIDLKVKLCGKYGILCEVTKIDESKNDDEIFQIVENIFSNESVGGGIIQLPLPRKSLE
                     KVLDFIPIEKDVDVISTEGKKRFYSGDFSKLSPVVRAFENFLSNCDINYNGLKAVVIG
                     EGDLVGKTISFYLSKKGASVETISSYKSGDKLDCELLVLATGIPNLIKGEAISVDCNV
                     VDFGSSVIDDKCVGDLDMSSSINHLGCISKSPGGMGPLVVRYLLLNF"
     gene            complement(8166..8780)
                     /locus_tag="P5014_00830"
     CDS             complement(8166..8780)
                     /locus_tag="P5014_00830"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HRY56687.1"
                     /translation="MIQLLFQNPALFLLISVSLVISISIHEFSHALVANYLGDGTAKS
                     MGRLTINPKAHLDPVGTILLLFVGFGWGRPVPFNPLNLKNPKRDAALISLAGPMSNLI
                     LAVVLSLLSKLFVVGGLMWIFLYFLVLYNLMLGFFNLIPLGPLDGNKIVFGFLPNRFA
                     VQWIEFQKYSMYILIVMVALGFTGKILGPLVDISIKILGFNLAL"
     gene            8877..9071
                     /gene="secE"
                     /locus_tag="P5014_00835"
     CDS             8877..9071
                     /gene="secE"
                     /locus_tag="P5014_00835"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HRY56688.1"
                     /translation="MCGVNRVSKFVKESFEELKKVQFPNKKETLRLTGYVIGISLVTG
                     VLVTLLDYLFKEFLTFIITK"
     gene            9074..9853
                     /gene="nusG"
                     /locus_tag="P5014_00840"
     CDS             9074..9853
                     /gene="nusG"
                     /locus_tag="P5014_00840"
                     /inference="COORDINATES: protein motif:HMM:TIGR00922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HRY56689.1"
                     /translation="MDDKNNTQINNDDTDLENKDSEMQEEATEQGEDNGHTEEEINEN
                     EEGSDVAEDATASEEEKIDENTSQDDEVDEENVIVINKNVSQSASWYVIHTYSGHESK
                     VALSLKERVEAAGHSDKVFKIFLPHQQKIVVSEGKKRSVDEKLFPGYLVVLMDMNDDS
                     WYIVRSTPGVTGFVGMGSTPTPLPKSEVLTLMKFAKMEAPKFEAKFRLGDSVKVNEGP
                     FKDFLGKVDEVNEEQGKVKVLVSVFGRETPLELSFTQVSQL"
BASE COUNT         3291 a         1767 c         1680 g         3065 t
ORIGIN      
        1 ttaaatcgac ttcagatcaa agaactcaaa ctttctctct ttttcttttc tttaggcatt
       61 gggttagtcc agctactcca cttgcaatca gggtatctgc tgcaaccgta aaaaaccttc
      121 ttttttgcct ttttgacaac aatatcaccc tccccacact caggacattt cattccaatt
      181 ttatcttgat agtttttggt agttttgcat tttggataat tactgcaagc taaaaatttt
      241 ccaaatctcc cttgttttaa gacaaaaact ccatcttcgc aattagggca tttaccgtgg
      301 tcaacgctct cgcctgtttc ttccgaacca acctccccag ctaaaggttt agcatattta
      361 caatcgggat atcccgagca acttaaaaat ttcccgtact tccctaattt aaaaaccaag
      421 gtttttccgc attcgggaca tttttcatca ctctcaccaa ggttagtgac gtcagcctta
      481 ctcaatgagg tttccttttc agctaattcc ttttcaaaag gtatataaaa atttttaatt
      541 aacggaaccc actccttctc gcctgaagca atttcatcaa gtgactcctc catttcagct
      601 gtaaactgat aatcaacaat atcaggaaaa tgggatacta aaagatcatt tacgacgtaa
      661 gctacatctt ttggtataaa atacctgccg tctttttcta cgtaaccccg ggcttgaatg
      721 gtactaatag tgggtgcgta agttgaaggt cttccaaccc ccaactcctc catttttttt
      781 attaatgttg cgtcactgta tcttgctgga ggttgagtaa aatgctgatc agaatttagt
      841 tcttttaaat ctaccctgtc gttttctgaa acatcgggaa tagaattcat ttcctctaca
      901 ccttcttctg aacccccgtt attttccgca tttccagaca taccaaggta ttttccaacc
      961 actaaccagc cgtcaaattt tatctgtgat cccacggatt taaaggtgta ctgtttactt
     1021 gaatcaattt gaacagttgt ctggtcgtac actgccgcac taacttgaca ttcaaccgct
     1081 ctcttccaga ttaccgagta aactttcgat tcatcgggtt taaatttttt attacttttt
     1141 ccgggaacaa gggataaatt agtgggtctt atagcttcgt gtgcttcctg agcatttttt
     1201 gatttggttt tataaaaagt aggtttttca ggtaaatact ctgatccgaa agttccttgt
     1261 atatagtttc ttactgtgga cacgaaagca gatgataaat ttaacgagtc tgttctgtga
     1321 taagtaatgt acccctgttc aaataaactt tgggctgcag acatggtcct tttagctgta
     1381 aaaccaaaaa gattggacat agtttgctga agcatagaag tgcttaaagg aggatagggc
     1441 ctgctacttc tctctgtttt tttaactgat gtgattataa aagtatcgtc ttttagctct
     1501 ttttcaattc cttgagcttc ttcttttgta gaaatactaa tttttttgcc tgctttttct
     1561 atcagtgtag ccttaaattt atcctggcta ccaacatcct gaaaaatccc cgccattgtc
     1621 caatattcct ccgatttgaa ggcttgtctt tccctttctc tttcaacgat cagtcttaca
     1681 gcaacacttt gaactcttcc cgcagaaagt ccgtacatta cttttttcca taaaagagga
     1741 gaaagtttat aacccacaag cctatctaga attcttcttg cttgttgggc atttactaaa
     1801 ttcatatcca cctgacctaa gtttttaaat gcttcattaa ttgcagattc agttaattcg
     1861 tgaaaaacaa ctctaccgaa ctttactttt tctcctgttg tagaattatc gattgtatct
     1921 tcaccttcaa tctctttctt tttagttttt aatttctttt ttgttggctg aagtaggtat
     1981 tcaagatgcc acgcaattgc ttccccttct ctatctgggt ctgtagctaa aattatttca
     2041 tcaatatctt ttgatttttg cttgagagtg tttaaagttt tttttgcttt cgggggaaca
     2101 atgtattcag gttcaaaatc tttttcaaca tcaacgccta aaccactttt gggaagatcc
     2161 ctgatgtgac ccatcgacgc ttctattatg taatcctttt tcggcaggat tgtcgaaaga
     2221 gtcttagctt ttgtgggcga ttcaactatt accaatttca tagcattttc ataaatgata
     2281 ccacgaagaa atttttgtca agaaaaacat aatatccaat ttattagata tgttcatatc
     2341 gtttgcaaaa ataagatttt tttactaata taggcaaaag agcataaaaa ggaggtagag
     2401 atgactttgg ataatatgaa aattataata tgtgtcacgt ttacgatttt gttgatatct
     2461 catcttggat attcgagaag tttaccggga gaaaaccaaa attttaaaac caaagctcaa
     2521 aaaattataa actcggtttg tggtacggtc cgagcggtat cagcattttg gtttatcgtg
     2581 ctgtttgtat ttttttgttt caatttgatt tcttaaattt atgccccgag gctctggtgt
     2641 ttaccccaat tacactagag ccttaatttt atattacaaa cctcacacgt gataaaatac
     2701 ccttatgtca gtgaagaaaa tagtaacagt accaaatcct attttaaata aaaaaaccca
     2761 aaaaattggc gaaataactg atgatattaa aaatacagct cgagatttaa tagataccgt
     2821 taaagttgcg gaggatcccg aaggtgcggg tttagcagct actcaaatcg gagtctcaaa
     2881 aagaatgtgt gtggtaagaa attttttcga agacccttta aatccaaatg aaataaaatc
     2941 aaacgatata gtattgataa atcccaagat tactaattac tctgatgaaa aagaccttga
     3001 ttgggaaggc tgtctaagtg ttcctgataa atacggtaag gtagaaagag ctcttaagat
     3061 aaaggttgct gcaaaagata tcgacgggaa tataattaaa ataaaggcag aaggattttt
     3121 cgctagaaca atacagcatg aagttgacca cttggatggc gtcctgttta catcaaaaat
     3181 tgagggtgaa acgttatcac aggaagagtt tgaccaaatg attgaggatc agaataatga
     3241 acaaggttga taaaccgatc aaaatagtgt tctttggcac atctgataga tcgttgccaa
     3301 tattaaacag tttaaaagaa aatttcaatc ttgttttatg tgttactaaa tcagatacag
     3361 tagttgggag gaaaaaatca atcagggaaa cagaagtcaa gaaatgggca gaacaaaaca
     3421 aagtcgaatt aattacagta ggctctttga aaggtacaga cctagagcgt acgttaagta
     3481 tcattgaaaa acacaaaccc aacctcggag ttgtcgctga ctttagtttc atgattccta
     3541 aatctattat agattctttc aatggaaata ttatcaacgt acacttctct ttattaccta
     3601 aatatcgagg agcaagtcct gttcaacacg caattttgaa cggagatgag ttaacaggag
     3661 taacttttta tttactaaat gagaagatgg atgaggggga tattttaaac caatacgagt
     3721 acaaaataga ttcaaaaata acctctggtg aactttactc tgttcttttt aaattatctg
     3781 cagaaaagtt accacaaacg attgttaact ttttaaaagg tgacgtaaaa cccatgcaac
     3841 aagatcattc taaatcatcc tatactattt caaaaacaaa ccctaaacac acctttattt
     3901 tcaaagaaga ttccttgata aactggtcag atggtcctaa taaaatacac cgagcagtca
     3961 gagcttttaa tccttggcca attgcttgga catatctgga agatttagaa aagacagatg
     4021 taatttcaga gaaaataaaa cttaaagata atgtaaataa aaagttaaag gtaaaaatat
     4081 tcacatcttc tattgaaaat ggaaaattga aaataaacca acttcaatta gaagggaaaa
     4141 ataaagcaga ttgggtaagt tttttaaatg gttattgtga aaaatcgaag taaaaaatcg
     4201 ttactctttg ttctccgtac cttcagattc ttttattaac ttatctatat cagcctgatc
     4261 ttttttaatt ctttttagac aagaagagca aagtctaagt ttaacagatc ttccgtcaat
     4321 taaaaatctt ttaactctaa ggttaataga atttctactt attgttcttc tggtaacacg
     4381 attaccaatc cctcttggga ccaaatttcc tttttgataa gtttttccac aaatttgaca
     4441 ttgtttagac ataacattgt gaattaaaac acacttgatt atttaaagca ataaaaagaa
     4501 cccgcaaaaa aagccggaaa tgaatttttc aaaatccagc ttttttgttt ttagtttacc
     4561 aattcattat tttttgttag ctacatcagc aggaaacaaa actctctgtc atgcaacata
     4621 ttaccttgtc gcatctcttt gatactttct ttggacacag gtcgcaacgt ctaacctccg
     4681 tttctccaaa taaagaatta agatctagat cttcatcaca gtcgtagcat tccgggtcaa
     4741 tatgataaat cgacgctact gtaggtgcta aacctctata acgaccacta acaagcactc
     4801 gacacatttt gctctctcca atcgcaaaaa tatttttaaa tactattggt aaactttaac
     4861 ccgatattat cactacgggt gcttccaatc aatatttttc ttagttaaaa agatatttga
     4921 aaaatgtaga ttattaattg tctgctatga tagaatctta aagctttaaa ggttgctcat
     4981 ttgaaatatt aatatagtaa aatataagtg tatgccgtct tagctcaatg gcagagcagg
     5041 cgctttgtaa gcgtcaggtt gtgagttcaa ctctcacaga cggctccatg ccgagatacc
     5101 aaagtggcca actggagctg actgtaaatc agctggctta tgcctacggg ggttcgaatc
     5161 cctctctcgg caccaaatta aagaattgaa aattagataa tccctgagat aggtgataat
     5221 ctaaaaacct aaccaaggat gagaatgtcg gagcgaacga agtgagcgag acggggtcgc
     5281 gagctttaca gattgtttga gccttggcga aatacaagat gttaagacgc gtgaccgaat
     5341 ccctctctcg gcaccaaagg taaacttgaa aattaacttg ttcttggaaa tataaaaacg
     5401 ttctcaagat ttaacaaggg atgagaatag cggactgagc gaaagcgaag gagctgtgcc
     5461 gaatagccaa agagctctta cgaacgaagt gagttagaga tatttggact agtggacgaa
     5521 tccctctctc ggcaccaaat taaagaattg aaaattagat aatccctgag ataggtgata
     5581 atctaaaaac ctaaccaagg atgagaatgt cggagcgaac gaagtgagcg agacggggtc
     5641 gcgagcttta cagattgttt gagccttggc gaaatacaag atgttaagac gcgtgaccga
     5701 atccctctct cggcaccaag atagagattt gaagatcatt ttttgaaaat ttaatttaat
     5761 tttcggatac aaatacaaaa gaaaggtggt gagatttaaa aaatatgaaa ggttttatag
     5821 attttattag aaaacaaggt gtagtaggtt tagcagttgg ttttatatta ggcggtgcag
     5881 tatcaaaagt tgtagcatct atagtcgctg atattatcaa ccctatctta gggttaatct
     5941 taggagcagc gggtggtctt cagcaaatgg ttataaaaat aggatcggca caattgttaa
     6001 taggtaactt tattgctact ttgatagact ttattgtaat tgctctggta gtttactacg
     6061 gagtaaaagc tttgaagtta gataggttag ataaaaaaga agacaaaaag taatcgattt
     6121 attaatatta gaaaatctgc ccgtgtgcct gggtagttca gtggtagaac gcttccatgg
     6181 taaggaagaa gtcgagggtc cgactccctc cccaggctcc agaaataccc acattttaat
     6241 aattaattca gaataaccta ttcattctct tacgtggaaa atatttaaat caatctacga
     6301 taataaccta tatcgatatt ttattactag ggtaaataaa aaaaatcctg gggacagacc
     6361 attacactat gtgtagttgg gactgccacc aggatttgta aaaaaaatcg ccgttttttt
     6421 tgacctaaaa gatttacaga ataaggtaaa aaactaaaac taaggcggat gcattttcac
     6481 tcctattcta ttctaccagt tcctatcagg gggtacgatc gataaatata cctgttaggt
     6541 tgaaatattg tttataatac tacactacag tttctttttt gtcaactatg ggtccttttt
     6601 attaaggaga tttattgatt taaaagtaag ttcaaaatat tagctatcta gttatcagag
     6661 tgagaataaa aaaacctgga gataaaaacc atcgtagtag tgctcgatgt atctccagga
     6721 attgcaaacg tgtcccattt ttaaccaaac aactaacgac tatagattac cagaacaaac
     6781 taacaactaa gtccagtcca tttgtctcct ttctaagcct agttcctgat agatagcata
     6841 atcaaaatat tttttccatc aggtcagtat attgtttaaa atactacacc aatgtttctt
     6901 ttttgtcatc tacggatatt tttattgatt taaaaaattc aaaagcaaat atctcactac
     6961 tagaggtccc atacccccag gggattttnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7081 nnnnnnnnaa aattcaaaag caaatatctc actactagag gtcccatacc cccaggggat
     7141 tttgaaatac aacctaaatg atttatagaa gaactcatat ccaaatctcc tacacattta
     7201 tcatctataa cagaagaacc aaaatcaacc acgttgcaat ccacggaaat ggcctctcct
     7261 tttattaaat tcggaatacc tgttgccaaa actaaaagct cgcaatcaag tttgtccccg
     7321 ctcttataac ttgaaattgt ttcaacacta gccccttttt ttgataaata aaaagatatt
     7381 gttttgccta ctaaatctcc ctcccctatt actacagcct tcaacccatt gtaattaata
     7441 tcacaattag ataaaaaatt ttcgaaggcc cttacaacag gtgacaattt tgaaaaatct
     7501 cccgaataaa atcttttttt accttccgta gaaataacat caacatcctt ttcaatcgga
     7561 atgaaatcta atactttttc caaacttttt ctaggtaagg gaagttgaat tattccccca
     7621 cccacactct cattagaaaa aatattttca acaatttgaa aaatttcatc atcattttta
     7681 gactcatcaa tctttgtaac ctcgcataaa attccatatt ttccacacag cttaactttc
     7741 aaatctataa acttatttga agattcgttg tttccgacct gaacaattga caacaccttc
     7801 cccgctccgt tacctttatc taaagcctcg gatatatttt tctctaaaat ttctgattgt
     7861 atttttgaat caaatatttt cacgtaatga ttatatcagg ggaaaagaaa aacctcgcga
     7921 ttgcgaggta tgagtttgtc ccgatgtgtc gatccaccaa gatcggatct ccgtttgagt
     7981 ttaggtgggt gtctgcgatt taaaactttc gaattaaact ttacgactcc catcgaagat
     8041 cgaatgatgg aagatttaca cgaccttcgg tttacccgag cacatcaggt ttgctcgttt
     8101 gagcattaat aaaataatac tcattaataa aaaattagtc aaattaaggg tgtctgtatt
     8161 tgcctttata aagccaaatt aaagcccaat attttaattg atatatcaac caaaggcccg
     8221 aggatttttc cggtgaatcc caaagctacc atcactatta aaatatacat actgtatttt
     8281 tgaaactcaa tccattgaac agcgaatcta tttggcaaaa acccaaaaac tattttattt
     8341 ccgtcaagag gccccagtgg aatcaaatta aaaaaaccta acattaagtt atatagaact
     8401 aaaaaatata aaaaaatcca catcaaacca ccgacaacaa atagttttga tagaagagaa
     8461 agtactaccg ctaatattaa atttgacata ggtcctgcta aagaaattaa agcggcgtct
     8521 cttttaggat tttttaagtt aagagggtta aatggaacag gtcttcccca cccaaaaccc
     8581 acaaacagta ataaaattgt tcccacagga tcgaggtgag ctttagggtt tatagtcagt
     8641 ctacccatac tttttgcagt accgtctcct aaataattcg ctacaagagc atgtgaaaac
     8701 tcgtgtatag atatggagat tactaaagat accgatatca gtaaaaaaag tgccggattt
     8761 tgaaataata attgaatcat atgtagtgaa gattatacat cagtgtgtaa atttttcttg
     8821 agttgaataa atttatataa ataaggttgt tgtgaacata aaataatcgt gttaaaatgt
     8881 gcggcgtgaa cagggttagt aaattcgtaa aagaatcttt tgaggaactt aaaaaggtac
     8941 agttcccaaa taaaaaagag actttaagac tgacaggata cgttatcggt ataagtcttg
     9001 tgactggtgt tctagtaaca ttactggact accttttcaa agaattttta acctttataa
     9061 taactaaata aatatggatg ataaaaataa cacacaaata aataatgacg atacagacct
     9121 tgaaaacaag gattctgaaa tgcaagaaga agcaacggaa cagggagaag ataacggtca
     9181 caccgaagaa gaaataaatg aaaatgaaga aggcagtgat gttgcagaag atgctactgc
     9241 gtcggaagaa gaaaaaatag atgagaacac atctcaggac gatgaagtag acgaggaaaa
     9301 tgtaatcgta ataaacaaaa atgttagcca aagtgctagc tggtatgtaa ttcatactta
     9361 ctccggccac gaaagcaaag tagctctttc actaaaagaa agagttgagg cggcaggaca
     9421 ttcggataaa gtttttaaaa tattcttacc acatcaacaa aaaatagtcg tttcagaggg
     9481 aaagaaaaga tcagttgatg aaaaattatt ccctggctac ttagtagtct tgatggatat
     9541 gaacgacgat agctggtata ttgttagatc tactccggga gtaaccggtt ttgtgggaat
     9601 gggttcaacc cctaccccat tacctaaatc agaggtttta actttgatga aatttgctaa
     9661 aatggaggct cccaagtttg aagctaaatt cagattagga gattcggtaa aggtaaatga
     9721 gggacctttc aaggatttct taggaaaagt tgacgaggtt aatgaagaac aagggaaagt
     9781 aaaagtattg gtttcggttt ttggaagaga aacaccgttg gaattatctt tcactcaagt
     9841 ttctcaatta taaaaaatgg cagtacaagc aaaaaaaggt aaaaaaataa aagcaatagt
     9901 aaa
//
LOCUS       DAOTFA010000011         9520 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate AMR_MDS_3115
            NODE_5088_length_9520_cov_5.688748, whole genome shotgun sequence.
ACCESSION   DAOTFA010000011 DAOTFA010000000
VERSION     DAOTFA010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868139
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (wastewater metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9520)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9520)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:36:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 536
            CDSs (total)                      :: 521
            Genes (coding)                    :: 520
            CDSs (with protein)               :: 520
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9520
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5088_length_9520_cov_5.688748"
                     /isolate="AMR_MDS_3115"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..209
                     /locus_tag="P5014_00845"
     CDS             <1..209
                     /locus_tag="P5014_00845"
                     /inference="COORDINATES: protein motif:HMM:NF020845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HRY56690.1"
                     /translation="GTPIIVDLPITMVFEIKYTEPGFKGNTVTNVYKDAEIENGTKVK
                     VPTFIKIGDKIKVDTRSGEYVSKA"
     gene            219..1265
                     /locus_tag="P5014_00850"
     CDS             219..1265
                     /locus_tag="P5014_00850"
                     /inference="COORDINATES: protein motif:HMM:NF013427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HRY56691.1"
                     /translation="MIPESINQGFGNISTILIFAVIGFICWVFILWGESKKDGFNTQR
                     FFDLSFISLIISGLLTYIIFRVVSWTKIYHPYHIILSVDEELLYSMSVFIISLFPIYY
                     YRKKYNWSVFRILDIYSLGFSILLLIVSIGRYLISGQREYAILFFILSVLYLLLIRRR
                     GYRFVSGMVFSVFCLFISVSLITFLRKGGSLLFASILFTIGVVNLYFRGKQNMSKNIL
                     PEHFLEKLKNKLINKDKRLSQQQKSLIQNDPYLQQGRATDNAETLDDVLEDTSKNVTD
                     AVLHNVKSVRVQVRKALARINIGKYGKCEVCGKPIDKARLEVYPEATTCIDCATDHSQ
                     IEEVKEDEMLEKNL"
     gene            1295..1579
                     /locus_tag="P5014_00855"
     CDS             1295..1579
                     /locus_tag="P5014_00855"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:NF013421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HRY56692.1"
                     /translation="MLFNQISLGLFIFSSLILLLSFIVFVKRSFKENIYVDIGWLLIS
                     IGGLSNIYEWARYGCVRDYLNFLNLFKFNVADFLISTGIILVVIGIWKKK"
     gene            1564..2262
                     /locus_tag="P5014_00860"
     CDS             1564..2262
                     /locus_tag="P5014_00860"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HRY56693.1"
                     /translation="MEKEIKIIFEDRNILILDKWSGLVVNRSNTYKETTLQDILEKTY
                     PELYLNVSDQEYLSRSGLVHRLDKDTSGIVAVAKNTDTFHYLLHQFKQRKTLKEYVAV
                     VHGEIEDEIIEIDAPLARNPKAPLRIAVVSGGKPAFTKIEKMYSKNIDGNTYTLVRVF
                     PKTGRTHQIRIHLSSIGHPVAGDKIYCASNLLENDDIVFSRLMLHAEILGFIDPENQK
                     FHRFKSPLPNEFKI"
     gene            2284..3351
                     /locus_tag="P5014_00865"
     CDS             2284..3351
                     /locus_tag="P5014_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HRY56694.1"
                     /translation="MKNSPAQRKYKSGSKTSINRSVRKRIFRIGLVLLVFVFSLGLLF
                     SYSFYKYLNQKFASASSSTSYSISDDLIPTVSYIVVEDLDSDPVVVKKVHFMIFNKSN
                     KKLSIYDLPVDKDYSLPGKFGNEILSRVFALSSLNSDDKFLSGANYVNRSILKLFGFK
                     VDKFLLTDESHEDFFDTLWRNGDVLSIKSLRDVAGLDGSFKTDIDIREFYDLMSFINS
                     LPSDRVVDDKNFPESFKNTGIFDNSIREITFDSAVAGEKKNISVLNGTNYDGLASFGA
                     RVIENYGGRVVATNSTARTYDKSVIITDDINSQTTGFLSRVFKIDNIVPKDQAGVYIE
                     NEVDRSDVVVIFGFDSSNDLY"
     gene            3577..>5812
                     /locus_tag="P5014_00870"
     CDS             3577..>5812
                     /locus_tag="P5014_00870"
                     /inference="COORDINATES: protein motif:HMM:NF021897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretion system DNA-binding
                     domain-containing protein"
                     /protein_id="HRY56695.1"
                     /translation="MEIKNYLALFLGFFAVVLGIYLRFKPKKSKSTGSSGSERVVLEV
                     QLSRNDEEKRDIQASSLSAENMFSALHGLLKDDPSIQESLSFEMAASGERGIRFYVTT
                     PASVLSFVESQIYAQYPTSSINIVSDYTPAINTSHTDYKVSSIVFSKPDYYPIKSFRD
                     FETDPMSSISSSVSNLKPGEEVWVQVLSRPIADKWQQDGFDYVNKVREGQDPGKTKSA
                     YNIFFDVIKKELFEITAGVLTGLFSRPTDGTEVKGKYITSAPKISAAKETELKAIENK
                     LSRMGFETVIRAVSASTQPDRVDANLRSITASLEQFSSTSLNSFLGKNEDNKQSALDA
                     YKKRDFDSERSIILNTEEMASLYHFPLSHIDTPNISWVYSRKSEPPTNLPIENCTYIG
                     ETMYRNQSVKFGIRNDNDDRLRHLYVIGKSGTGKSSLFVTMLSQDIANGQGAAILDPH
                     GETIENILDRIPDNRVEDVIYMDPSDTEFPVALNLLEIDDPSQKNLMASGLVSAIKQH
                     FDFSWGPRLEYLLNYCVLTLLEVPGTTMLGITRLLEDQNYLNYILHFVRDPLVQKFWS
                     EEFKQMKGNQKLVTEAISPIQNKVNRFLASTTIRNILGQRKSTIDIWDAMNSGKILLM
                     NLSKGKIGQDNANLLGALLVSRIQFYALQRARIPNDKRRPFYLYVDEFQNFATGSFEE
                     ILSESRKYKLGLNLTHQFTGQLPENLLKAVYGNVGTIMTFSLGAPDAKELAKEFAPYF
                     GEEDI"
     assembly_gap    5813..5912
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5913..6231
                     /locus_tag="P5014_00875"
     CDS             <5913..6231
                     /locus_tag="P5014_00875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56696.1"
                     /translation="KVPFASLERVFNENKESSVHVRPEVKELNEKTNLKVSTVKKPIK
                     DKQIVKKPSGPISHGDKSTKFVKKEQDEKEIIVKNVEKPLNKKEKPKTEYIDNIMGDD
                     DLR"
     gene            complement(6236..6928)
                     /locus_tag="P5014_00880"
     CDS             complement(6236..6928)
                     /locus_tag="P5014_00880"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRY56697.1"
                     /translation="MSFSLAVLTKKAIEASLKQKWAEAISLNSDILKAYPNNIDAKIR
                     LGRCYLQTKDFTKAKKMFKEVLEKDPINSIALKNYDLAKNQKTECNGNGTKLSTKALL
                     KEPGTSCEAVFQVISKNVKKGDITSGEELPVKIKKNKVEIYFDRKKKSILIGEITDEY
                     IAQRLTCAVEKHSNIKVTYIKWDDKDITVLIKTSVPIFKPDKVEIRPYLRKGTIEEPE
                     MEIEIESEEEIE"
     gene            7033..7572
                     /locus_tag="P5014_00885"
     CDS             7033..7572
                     /locus_tag="P5014_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012583963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRY56698.1"
                     /translation="MSGHSKWANIKRKKEVTDAKKSKIFSKMSRLISVAAKNGADVDS
                     NPTLRLAVERAKEARMPKENIDRAIDKGSGQGGSVSFFETIYEGFGPNGEAFYIKALT
                     DNINRTVAEVRNIFSKHGGSLGGLGSTAYIFSPDPDNPSYTIEVDSSAAKSLEGILDE
                     LDDNDDVQDVFVNFQLPEE"
     gene            7569..8069
                     /gene="ruvC"
                     /locus_tag="P5014_00890"
     CDS             7569..8069
                     /gene="ruvC"
                     /locus_tag="P5014_00890"
                     /inference="COORDINATES: protein motif:HMM:TIGR00228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HRY56699.1"
                     /translation="MIIIGIDPGTAKMGYGIIKVDDRGKKVSLLESNVLITHPDLEPQ
                     NRLKFLYDSLSDILVKFKPDLMVIEKLFFNTNAKTAIAVGQARGISMLSAAKKKVKVS
                     EYTALEAKLALTGYGRSDKKVMQEAVKKTFKLKDKIKSDDANDAVAMALCYVKKVLEE
                     PCPKKK"
     gene            8071..8880
                     /locus_tag="P5014_00895"
     CDS             8071..8880
                     /locus_tag="P5014_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56700.1"
                     /translation="MFKPIKIELAKKNKYFIQSVLVGIFIYITTQNLFDLEGIKLALL
                     SLILVVSGSLIAHSTGITKENFIYSVIMPLGVISGGVLSLKFYPNLGSVFKIFVVAFF
                     SGMYYLVSLADNIFLVVSDREEIIPLYRVAVTWSQILQVIVAIPLFAGIFKLNIVTFT
                     HSLIISLISFLFTYYQLWVYRFEPDAKKVGEGEKFYICFLSFFIVFVTSLGVSFFPSE
                     DFLRALFTSSVLLFVLNYISAHLKNEISRRIIIRHLLITVVFLILLIFFKP"
BASE COUNT         3121 a         1422 c         1739 g         3138 t
ORIGIN      
        1 aaggaactcc aataattgtt gatctcccta taactatggt ttttgaaatt aaatacactg
       61 aacccggctt taagggaaat acagttacaa atgtctataa ggatgcagaa atcgagaatg
      121 gtacaaaagt aaaggtgccg acattcatca aaatagggga taaaataaaa gtagatacca
      181 gaagcggtga gtatgtatct aaggcttaaa aattaaaaat gattcctgaa agtataaacc
      241 aaggatttgg aaatatcagt actattttaa tattcgctgt tataggtttt atttgctggg
      301 tttttatact ttggggtgag tcaaaaaaag atggttttaa cacccagagg tttttcgatt
      361 tgtcttttat ctccctaata atttccggtt tattaacata tattatattc agagtagttt
      421 catggacaaa aatttatcat ccctaccaca tcattttgag tgtagatgaa gaactgttat
      481 acagtatgtc cgtcttcatt atttcgctgt ttccgattta ttattacaga aaaaaatata
      541 attggtctgt ttttagaatc ctagacattt attctttggg attttcaata cttctactca
      601 ttgtgagtat cggtaggtac ttaatatcgg ggcaacgaga gtatgcaatt ttgtttttta
      661 ttctctctgt cttatatttg ctacttataa gaagaagggg atacagattt gtttcaggta
      721 tggtattttc tgtcttttgt ttgtttataa gtgtttccct tatcaccttt ttaagaaagg
      781 gtggctcttt actattcgct tcaattctgt ttacaatagg agttgtaaat ttgtatttta
      841 gaggtaaaca aaatatgtca aaaaatatat tgccggaaca tttcttagaa aaactaaaaa
      901 ataaattaat aaataaagat aaaagactct cacaacagca gaagtctttg atacagaacg
      961 atccctatct tcaacagggt agagcaacgg acaatgcaga gaccttggat gacgttcttg
     1021 aggatacaag taaaaatgtt acagacgcag tgttgcacaa tgtgaagagt gtaagagtcc
     1081 aggtcagaaa agctctagca agaattaaca ttggtaaata tggaaaatgt gaggtttgtg
     1141 gaaaaccgat agataaagcc aggctcgagg tttatcccga ggctacaact tgtatagatt
     1201 gtgctactga tcattcccag attgaagagg ttaaggaaga tgagatgtta gagaaaaatt
     1261 tatagtgttt gaagcaacct acaattgtaa ttatttgtta tttaatcaaa tatccttagg
     1321 attgttcatt ttttcttcgt taatattgct tttatctttt attgtatttg taaaaagaag
     1381 ctttaaagaa aatatttatg tcgatattgg atggttgttg atatctatag gcgggctaag
     1441 taatatttat gaatgggcaa gatacggttg tgttagagat tatctaaatt ttttaaattt
     1501 gtttaagttt aatgtagctg atttcttgat ttcgacaggt ataatcttag ttgttattgg
     1561 aatatggaaa aagaaataaa aataatattt gaagatagaa atattttaat attggacaag
     1621 tggtccggat tggttgtaaa caggtcaaat acttacaaag agactacgtt gcaagatatt
     1681 ttagaaaaaa cataccccga gctttatttg aatgtatccg atcaagagta cttgagcaga
     1741 tcgggattag tacacagact agacaaagat acctcaggta ttgtagcagt tgccaaaaat
     1801 acagatactt ttcattatct tttacaccaa tttaaacaaa ggaaaacctt aaaagaatat
     1861 gttgctgttg ttcatgggga gatagaggat gaaattattg aaattgacgc acctttagct
     1921 agaaatccta aagccccgtt acggatagct gttgtatcag ggggaaaacc tgcttttact
     1981 aaaattgaga aaatgtattc caaaaatatt gatggtaata cttacacttt agtaagggtt
     2041 tttcctaaaa cgggcaggac acatcagata agaatccatc tgtcatctat cggacatcct
     2101 gtagcaggtg ataaaatata ttgcgcatct aatttactcg aaaacgatga tattgttttt
     2161 agtaggctaa tgttacatgc cgaaatatta ggttttattg accccgaaaa tcaaaaattt
     2221 caccgtttta aatccccctt gccaaacgaa ttcaaaatat aatatgatat atcagatata
     2281 tctatgaaaa attctcctgc ccaaaggaaa tataagtcag gttccaagac atctattaac
     2341 cgctctgtcc gaaaaagaat atttaggatt ggtttggtgt tgttggtgtt cgtattctcc
     2401 ttaggtttgt tattttcata ttctttttat aaatatttaa atcaaaaatt tgcttcagct
     2461 tcatcttcaa ccagttattc aatatcagat gatttaattc ctacagtttc ttacattgtt
     2521 gtggaggatt tagatagtga tccagtggtt gtaaaaaaag tacatttcat gatttttaat
     2581 aaaagtaata aaaagctgtc tatatatgac ttaccggtag ataaggatta ctcattacct
     2641 gggaagtttg gcaatgaaat tttaagcaga gtttttgcct taagttcgtt gaattcagat
     2701 gataaattct tatcaggagc taattatgtg aacaggtcta ttttaaaact ctttggtttt
     2761 aaagtggata aatttttact gactgatgaa agccacgaag atttttttga caccctttgg
     2821 cgtaatggag acgtactaag tataaaaagt ctacgtgatg ttgctggttt agacggctct
     2881 tttaaaaccg atattgatat aagagagttc tatgacctta tgtcatttat aaattcatta
     2941 ccttcggata gagtggtgga tgataaaaat tttcccgaat cttttaaaaa tactggtatt
     3001 tttgataatt ctataagaga aattactttt gactctgcag tagcagggga aaagaaaaat
     3061 atttcagttc ttaatggtac taattacgat gggcttgcct cctttggcgc tagagtaata
     3121 gaaaactacg gaggtagggt agtggccacc aacagtaccg cgagaacata tgacaaaagt
     3181 gtcattataa ccgatgatat taattctcag acaaccggat ttctatccag ggtttttaaa
     3241 attgacaaca ttgtacctaa ggatcaggca ggtgtttata tcgaaaatga agtggataga
     3301 tcggatgtgg tggtcatttt tggatttgac agttctaacg atttgtatta gtatattcac
     3361 agtactctct cctttctatc ccccgtccca aattagggcg ggggtttatt ttattcccca
     3421 aagaaaagtc tgatttcttt gtttaacttg gtttttaggt ttttcttttt ccatcggact
     3481 cgctccaaaa ggagttcgtc agagggcggg gggttatttt attcccctaa attaaattgt
     3541 ttactgattg tagttatagg tttttaatat aaaaatatgg aaataaaaaa ttatttagct
     3601 ttgtttttag gtttttttgc tgtggtttta ggaatatacc taaggtttaa acccaaaaaa
     3661 tccaaatcaa caggttcttc gggatccgaa agagtagtct tagaagtcca actgagcaga
     3721 aacgacgagg aaaagagaga cattcaagca tcttctttaa gcgctgaaaa tatgttttcc
     3781 gctttacacg gtcttttgaa agacgaccct tctattcagg aatcattatc ttttgaaatg
     3841 gcagcttcgg gagaaagagg gatacgattt tatgtcacaa ccccggcctc tgttttgagc
     3901 tttgtagaaa gccagatata tgctcaatat ccaacttcaa gtattaatat agtctccgac
     3961 tacactcctg cgataaacac ttcccatacc gactataaag tttccagtat tgtattctcc
     4021 aaacccgatt attacccgat aaaaagcttc agagattttg aaaccgatcc catgtcttca
     4081 atatcatctt cggtgtctaa tttaaagccg ggggaggagg tttgggtaca ggttttatca
     4141 agaccaatag cggataaatg gcaacaggat ggttttgatt atgttaataa ggttagggag
     4201 ggtcaggatc cgggaaaaac taagagcgct tataatattt tttttgatgt cataaaaaaa
     4261 gaattgttcg aaattaccgc aggagttctt accggtcttt tttctagacc taccgacggt
     4321 accgaggtta aaggaaaata tatcacctct gcacctaaaa taagtgcggc taaagaaaca
     4381 gagctaaaag ctatcgaaaa taaactatcc agaatgggtt ttgagacagt tattagggct
     4441 gtttcggcat caacacagcc tgatagagta gatgctaact taagatcaat aactgcttcg
     4501 ttagagcaat tttcaagtac aagtttaaat agttttttag gtaaaaatga ggataataaa
     4561 cagtctgctc tagacgcgta taaaaaaaga gattttgatt cagagagatc aataatatta
     4621 aataccgaag aaatggcctc tctgtatcac ttccccctct cacatataga tacccctaac
     4681 atttcttggg tttactcaag aaaatccgag ccaccgacta acttgccaat agaaaattgc
     4741 acatacatag gggagactat gtatagaaat caatcagtta agttcggaat tcgaaacgat
     4801 aatgatgata gattaaggca cctttatgtt attgggaagt caggtactgg aaaatcctct
     4861 ttgtttgtta caatgctgtc tcaggatatt gcaaatggtc agggagctgc aattttggat
     4921 cctcacggag agacaataga aaacatactt gatagaattc ctgacaatag ggttgaggat
     4981 gttatttata tggatccttc tgatactgag tttcctgttg cactaaatct tttggaaatt
     5041 gatgatcctt ctcaaaagaa tttgatggct tcaggtttgg tgtcagcaat aaagcagcat
     5101 tttgattttt catggggacc taggttagag tatttactaa attattgtgt tttaacctta
     5161 ttggaagtcc ccggtacaac aatgttaggt ataacaaggc tgctcgagga tcaaaattat
     5221 ttaaattata ttttacattt tgtaagagat cccctggttc aaaaattttg gtcagaggag
     5281 tttaaacaaa tgaaaggtaa ccaaaaatta gttactgaag ctatatcgcc aattcagaac
     5341 aaggttaata ggtttttagc atcaaccacc attagaaata ttctcggtca gagaaaatcc
     5401 accatagaca tttgggatgc aatgaactcg ggtaaaatat tattaatgaa tttatcaaag
     5461 ggtaaaatag gccaggataa cgcgaacttg ctcggagccc ttcttgtatc aagaattcaa
     5521 ttctatgccc ttcagagagc gagaatacca aatgataaaa gaaggccatt ttatttatat
     5581 gttgatgagt ttcaaaattt tgccaccggt agttttgaag agatactttc agaatcaaga
     5641 aaatacaaat tggggcttaa tttaacacac cagtttactg gtcagcttcc agaaaacctt
     5701 cttaaagctg tttacggaaa tgttggtacg ataatgacct tttctttagg tgctcccgat
     5761 gctaaggaat tagcaaaaga atttgctcct tattttggtg aggaggatat aannnnnnnn
     5821 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nncaaggtac cttttgcctc attagaaagg
     5941 gtttttaatg aaaataaaga atcaagcgta catgttcgac cagaagttaa ggaattaaat
     6001 gaaaaaacta atttaaaagt cagtacagtt aaaaaaccta taaaagataa acaaattgta
     6061 aaaaaacctt ccggtcctat cagccatggc gataaaagta cgaaatttgt aaaaaaagag
     6121 caggacgaaa aagaaattat agttaaaaat gtagaaaagc ccttaaataa gaaagaaaaa
     6181 ccaaaaacgg aatatattga taatataatg ggagacgacg atctgaggta attatttatt
     6241 caatttcctc ttcactttct atttcgattt ccatttcggg ttcttctata gtgccttttc
     6301 tcaaataggg tctaatctct actttatcag gtttaaagat aggtacagat gtttttatta
     6361 aaacagtaat atctttgtcg tcccatttta tgtaagtaac cttgatatta gaatgctttt
     6421 ctacagcgca cgttaatctt tgagcaatat actcatctgt aatttcacca attaggattg
     6481 attttttctt tctatcaaaa taaatctcca ctttattctt ttttatcttt acaggtagtt
     6541 cctcacctga ggtaatatcg ccttttttaa catttttaga aattacttgg aatactgctt
     6601 cacaagaggt tcccggctct tttaacagag cttttgtact taatttggtt ccatttccat
     6661 tacattcagt tttttgattt ttcgctagat cataattttt taaggcgatg gagtttattg
     6721 ggtctttctc taaaacctct ttgaacattt tcttagcctt tgtaaaatcc tttgtttgaa
     6781 gataacacct tccaagtcta atttttgcat caatattatt gggatatgct tttaatatat
     6841 cggaatttaa gcttattgct tcggcccatt tttgctttaa tgacgcctca atagcctttt
     6901 ttgttagtac tgctaggcta aaactcataa gtgatagcat ataaaaatat aaaaatttgt
     6961 caataagcga tttctaaaaa gttaataata tttttgaata aaaagtaatt aaagtgtgct
     7021 aatatatctt ctatgtcagg acattcaaaa tgggctaata taaaaagaaa aaaagaagtt
     7081 acggatgcta agaaaagcaa aatattttca aaaatgagta ggttaatatc agtcgcagca
     7141 aaaaatggtg ctgacgtaga ttcaaatcct actttaaggc ttgcagttga gcgcgcaaaa
     7201 gaagcaagaa tgcctaagga aaacatagac agggcaatag ataaaggctc gggtcagggt
     7261 ggttcggtta gtttttttga gactatctac gaaggttttg gacctaacgg agaggctttc
     7321 tatattaaag ctttgacaga caacataaat agaactgtgg ctgaagtgag aaatatattc
     7381 agtaaacacg ggggttcttt aggtggttta ggaagcactg cgtatatctt ctcaccggat
     7441 ccagataatc cgagttacac tatcgaggtt gattcttcag cggcaaaaag tttggaagga
     7501 atattagacg agcttgatga taatgatgac gttcaggatg tatttgtaaa ttttcaacta
     7561 cccgaagaat gataataatt ggaatagacc cgggtacagc taaaatgggt tacgggataa
     7621 taaaggttga tgatagagga aaaaaagtca gtcttctgga aagtaacgta cttattactc
     7681 accccgattt ggaaccccaa aatagactta agtttttata tgatagttta agcgatatac
     7741 ttgtcaaatt taaacccgat ttgatggtta tagaaaaact tttcttcaat actaatgcca
     7801 agacagctat cgcagttggc caagcaaggg gtataagtat gcttagtgcc gctaagaaga
     7861 aagtaaaagt aagcgaatat acagctcttg aagcaaagtt agctttaacc gggtatggca
     7921 gatccgataa gaaagtaatg caagaagccg ttaaaaagac atttaaattg aaagataaaa
     7981 taaaatcgga tgatgctaat gatgcggtag ctatggcttt atgttatgtt aaaaaagtat
     8041 tagaagaacc ctgtccgaaa aaaaagtagt atgttcaaac ctattaaaat tgaactagct
     8101 aagaaaaata aatattttat tcaatcagtc cttgtcggaa tattcattta catcactaca
     8161 cagaatctat ttgacttaga agggataaag ttagccttgt tatcattaat tttagtagta
     8221 tctggatctt taatagcaca ctcaacgggt ataacaaagg aaaattttat ctacagtgta
     8281 ataatgcccc ttggagtaat ttcgggtggt gtcttgtcct taaaatttta tccgaacttg
     8341 gggtcggttt ttaaaatttt tgtagtggct tttttttcag gtatgtatta tttagtttct
     8401 cttgcagata atatctttct agtggtaagt gatagggaag agataatacc tctttacagg
     8461 gtagcagtga cttggagtca gatacttcag gtaattgtag ccatacctct ttttgcgggc
     8521 atatttaaat taaatatagt tacatttact cattcattaa tcatttcatt aatatctttt
     8581 ttatttactt actaccaatt atgggtttat agatttgaac ctgatgcaaa aaaggtaggg
     8641 gagggggaga agttttacat ttgtttcttg tcttttttca ttgtatttgt tacttccttg
     8701 ggagtttcct ttttcccttc agaggacttt ttgagagcct tgtttacttc atccgttctt
     8761 ctgttcgttt taaattacat atcagcacat ctaaaaaatg aaatatcccg aagaattatt
     8821 atcaggcatt tattgataac agttgttttt ttaatattac ttatattttt taaaccttaa
     8881 ttatttttat ttttatttta atcattaaaa ttacttaatg ctttgtattg ttgttatata
     8941 agcaaatagt ggggaagtga aatagggata taccaacata cgttggaaca ctgttgtcac
     9001 actataggcg caagtgataa tactataggt cctatagtat ttaacataac ttaatatcag
     9061 aaattcctct gtagaattgt catatatcta agcagtaaga ttgtcatact aataatctgt
     9121 aagattgtca tacattctca gagtagggga tgtggataac tcatcgtgcg ttaatgtgta
     9181 aattaagagg aaataaagag atgtgttgtc tcaagtgcgt gaaaataaat aataattaaa
     9241 ttaaataaat aaattaaaaa acagagaata acattaatta attcacgtgc gtttattcca
     9301 cttcccctct tcgacagtct ccattgtcct cattaggcct tatttgataa aaacagcctt
     9361 acccctaaca agaagtcgtt aaacatacat tatacgacgt atcctgtgtt gagtttcgag
     9421 catccctatt tccaaaattc tctctaaaat taattcttat atttatgtga attattattt
     9481 gaaataaata cagtaaatat ttgcattcac ttctctatta
//
LOCUS       DAOTFA010000012         9231 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate AMR_MDS_3115
            NODE_5312_length_9231_cov_7.596883, whole genome shotgun sequence.
ACCESSION   DAOTFA010000012 DAOTFA010000000
VERSION     DAOTFA010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868139
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (wastewater metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 9231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:36:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 536
            CDSs (total)                      :: 521
            Genes (coding)                    :: 520
            CDSs (with protein)               :: 520
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9231
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5312_length_9231_cov_7.596883"
                     /isolate="AMR_MDS_3115"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..210)
                     /locus_tag="P5014_00900"
     CDS             complement(<1..210)
                     /locus_tag="P5014_00900"
                     /inference="COORDINATES: protein motif:HMM:NF013970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HRY56701.1"
                     /translation="MKRKTINEKLDDKVRLSKGVVIDTNILIRALIDENPELTKIISN
                     TDEVHIPLPIFFETIFVLEKFYKQSR"
     gene            complement(191..439)
                     /locus_tag="P5014_00905"
     CDS             complement(191..439)
                     /locus_tag="P5014_00905"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56702.1"
                     /translation="MEYATLTKQNQITVPIKVRDKLKLKPSDRLVFYESGETIKVSKL
                     KSLDDFKGLLKDSKIKLNKQMIESAWTERHIINEKKNN"
     gene            539..877
                     /locus_tag="P5014_00910"
     CDS             539..877
                     /locus_tag="P5014_00910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009197304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HRY56703.1"
                     /translation="MLKNIIKFIVNGFAVYATAYLLSGVTVSSFKVALIVALVLAALN
                     ILVKPILLILSLPVTIITLGLFTFVIDALMVIIVSKIVPGFSVDSFLTAIVFSIVLTI
                     ISYILHKLID"
     gene            complement(931..1806)
                     /locus_tag="P5014_00915"
     CDS             complement(931..1806)
                     /locus_tag="P5014_00915"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRY56704.1"
                     /translation="MKLDIAQKKFIEELKSEGKSGATVVAYNKDIEQLLSFLSSKKIE
                     EVEKISIEPIVAFMKDLGEKNYTPKSISRKTNAARTFIKYLAKEGYLKEDISSQLKHP
                     KFETGAPRILSKMEYRALRDSAKNDVRSYAMIELLLQTGITISELAGIEMKDFEIKDE
                     TASLFIPKRNNKESRNIPVNKPAVEAVKKYIEDERSKVSGASENLFITKTGKGLLIRN
                     IRSTIDRFFRIAGIEKAKVNDLRHTFVAFHLENGVSISYLAKVAGHKRESTTEKYLEY
                     IKKPEKMDKTELTTL"
     gene            1938..2339
                     /locus_tag="P5014_00920"
     CDS             1938..2339
                     /locus_tag="P5014_00920"
                     /inference="COORDINATES: protein motif:HMM:NF016839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HRY56705.1"
                     /translation="MSSISRLKYILGIVFFTVLSVNTIKSALNVLKSKDRLDQVNNEV
                     AVLEDEKKKIEEEIEFKKTDEYIEAKARNDLNLIKPGEKVYVVKTTEKDTAGNVLSET
                     DTAASEKGLDKGNEEKKNENWYSWYRLFFDN"
     gene            2395..2468
                     /locus_tag="P5014_00925"
     tRNA            2395..2468
                     /locus_tag="P5014_00925"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2427..2429,aa:Met,seq:cat)
     gene            2552..2627
                     /locus_tag="P5014_00930"
     tRNA            2552..2627
                     /locus_tag="P5014_00930"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2585..2587,aa:Met,seq:cat)
     assembly_gap    2740..2839
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2840..4294
                     /gene="ftsH"
                     /locus_tag="P5014_00935"
     CDS             <2840..4294
                     /gene="ftsH"
                     /locus_tag="P5014_00935"
                     /inference="COORDINATES: protein motif:HMM:TIGR01241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HRY56706.1"
                     /translation="LLMFGLPFLVLFYFLRQMKGQNGDIMSFGKSRAKLFVKGQQKIT
                     FNDVAGCEEAKREMAEIVDFLKFPTKYRKLGARIPKGVLLVGPSGVGKTLLARAIAGE
                     AEVPFFSVAGSEFMEMLVGVGSSRVRDLFAMAREKQPSLIFIDEIDAIGRQRGMGIGG
                     GHDEREQTLNQILTEMDGFDVRTSVIVLAATNRPDMLDSALVRPGRFDRRISIPLPDL
                     KDREEIIKIHMRGKPINADVSVEKLAKKTVGFSGADIENMLNEAAILTARENRESITD
                     KDLDEASLKVTIGSERKTLQSDEEKRMTAYHEAGHALVAVNVKDMDPVQRVSIVARGQ
                     TLGHTDLPPERDRYQLTKTRIESLITTMLGGRAAEEIVFGELTAGASDDIKRCTELAR
                     KMVTEYGMSSLGPIFFEGRSEFSWLAEEFGEGSSISEEMSSKIDKEISGIVEKSYQEA
                     KKILKENKEKLDKVVAKLLEVETIGGDEFKELVS"
     gene            4311..5102
                     /locus_tag="P5014_00940"
     CDS             4311..5102
                     /locus_tag="P5014_00940"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HRY56707.1"
                     /translation="MLIDSHCHLTHEFYENNLEELIKDSIELGISNFITIGTSVLENQ
                     KVIQVSENYENVFCTVGIYPHENQELTFDILKKSLEENINSSKKIVGIGECGIDITNR
                     DSQRKLEDQKSLFEMQLSLAVKYGIPVTVHNRNGDEIILNILKKYSSNNLRGVIHCFD
                     SNWSFAKNVLDLGFYISFSNMVTYPKKDALLEVVKNVPKDRFLIETDSPYLPPQSMRG
                     EINYPKYVKIVAEKVAQIKQKTFNEVSELSFKNTCSLFNICCQNL"
     gene            5084..6628
                     /locus_tag="P5014_00945"
     CDS             5084..6628
                     /locus_tag="P5014_00945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRY56708.1"
                     /translation="MLSKFVEKHDRLVSRILEIMLGILTWSLLLSPVWLGILFPQAVV
                     YVLTLLTVYWSYMAFTHTFGLFMGYKQYREEMATDWYLKCQKLDFSSLPEPETRPKYL
                     SDIRHMILIPVVNEPEAVLRDSIFSIFNQTLPTSQICMVFTVEQKYSEEVIERIKNII
                     GDKDKDLHSLMFFIHPAGIPGEAIGDGGANRAWGSSHAVEELKKRGDNLRNFIFSNID
                     SDHVLNKQYLSRLTYLYLTTDRRDNHFYSSAVPLFDNNLWRVPLMMRLEANSVTLGVI
                     SDWAVSKGKIRKCFSAFSVSLQTLIDANFFDVALGADDTIFYWRAFFARNGDFDGVGH
                     FIPYSADAVEGKNFRDSHRSLYKQLLRWGWGAVDFPLSIKTFLKDHNIEFAKKFRWVR
                     RHIKERVILTNIVFLITFGFAIVTLVNPYVKQSNFAYSLPKIMSFILTLTMSFMIPGV
                     YFRKKFSVAPPKEWGRFRKFLTYFEAPLITINLLTYSFFPTLHAQTMLIFGKRFKDLY
                     HTQKIR"
     gene            6631..8139
                     /locus_tag="P5014_00950"
     CDS             6631..8139
                     /locus_tag="P5014_00950"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HRY56709.1"
                     /translation="MKLNKRIEKTTEYILVKLKDKNLSNFLVNTIIDIFLFIYIRSRF
                     QYLNNDIPLWYTKIWGDAQLAPKFNLYLIPLVAMAVNVVALIFTLLNRYYLRYLEDVL
                     WYCVLFFNIFIVGSVLRIIRISSIPFSSFINPLYVKLLVPMSFSLIFMLITMPYFIDF
                     AKKKKLITNPQVHSHPGMILRAPSARGGGLLYSIILMISAIIFIGFSKQFSGLYISLI
                     MISVLSFVDDYQNTHPKSSYRFLENPVLRLFLLFLSVIPVVFSGVMIHTVSNPFGSFV
                     KLDVIEINIFNSSIPLISLLVTSLWIVWLMNVLSWSNGVDGQFPGIVGIASLLVALLA
                     LRFEELTDSHYQIAVFSAISAGAAFGSVRYNWHPSKIMWGFGAMSAGLVIASLAILAQ
                     AKIAVSVLILLIPFLDALFTVSRRILHGKNPFKGDRGHLHHLLLDKGWSVSKVAIFYW
                     LATALFGLVGLFSPERIVLKLTFLIMGVVAFGLIVLNISLNTRKKETPPTVL"
     gene            8184..8897
                     /locus_tag="P5014_00955"
     CDS             8184..8897
                     /locus_tag="P5014_00955"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRY56710.1"
                     /translation="MFVGKNIESVPKQKMRILLVSSDNKLIEKLNLNLRDCYIVDTAR
                     GASQAINLSETNYYDSIVVDTYLDDIEGLELCKMIRDYDVDSPTAYLSNKEDRSDRVM
                     CLDAGIDVIIPRYAGVEEILGQIHALARRNGNHKNGRNVLKIRNISLDMKNRIFYIDG
                     RCLHLRRKEFDLMEYLMINKGRAVSKEELLEHVWEKGLDVMSNTVEVHIRSLRKNFES
                     MLGEKIIRTNRGFGYEIET"
     gene            complement(8944..>9231)
                     /locus_tag="P5014_00960"
     CDS             complement(8944..>9231)
                     /locus_tag="P5014_00960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56711.1"
                     /translation="IEKEIKAKEIIIANKGDISPEAMALFFFTGCSLSLGASTKSLRK
                     YAPEDIAQNATKAFKDHEKTSPSNNVPAKNGAAKTLAFFSHCLGRAVLNMW"
BASE COUNT         2912 a         1397 c         1722 g         3100 t
ORIGIN      
        1 tctagattgt ttataaaatt tctccaaaac gaaaattgtt tcaaaaaata tcggtaacgg
       61 aatgtgaacc tcatctgtat tcgaaataat ttttgtaagt tccgggtttt catcaattag
      121 agctcttatc aaaatgttgg tatctataac aactccctta gacaatcgaa ccttatcatc
      181 taacttctca ttaattgttt ttcttttcat ttatgatatg tctttccgtc caagcacttt
      241 ctatcatttg tttatttagt ttaatcttag aatcctttaa taaaccttta aaatcatcta
      301 aagatttaag tttagatact tttattgttt cgccggattc ataaaaaacc aacctgtcac
      361 ttggtttaag ttttagttta tctcttacct ttattggtac tgttatttga ttttgtttag
      421 ttaatgttgc gtactccata ttaatgataa agtatcatat acattaaaaa tgtaaataat
      481 taatgaaaga acttcagata agttagttat gttattatat tagcaggggg tgaaattaat
      541 gttaaaaaat ataataaaat ttatagtaaa tggttttgcg gtttatgcta ctgcatatct
      601 tctttcagga gtaaccgttt cttcattcaa agttgcttta attgttgcct tagttttagc
      661 agccttaaat attttagtaa aaccaattct tttgattctc tcccttccag taactattat
      721 aactttaggt ctattcacct ttgtgataga tgctttaatg gtgattattg tctcaaaaat
      781 tgttccggga tttagtgtag acagtttctt aacagcaatt gtgttttcaa tagtattgac
      841 aataatttca tatattttgc acaagctgat tgattgaagt tagactaaaa attaaccttt
      901 ttaaaaaagt atttcgttac aaaatctatt ttagagtgtt gtcaactctg ttttatccat
      961 tttttccggt tttttgatgt attctaggta tttttcagta gtagattctc ttttatgtcc
     1021 tgcaacctta gctaaataag agatactcac accgttttca agatgaaaag ctacaaatgt
     1081 atgtctcaag tcatttactt ttgccttttc aatacctgca attctaaaaa acctgtctat
     1141 agtcgatctg atatttctaa ttaacaaccc ttttccggtc tttgtaataa aaagattttc
     1201 cgaagcccct gatactttag atctctcatc ttcaatatat ttttttactg cctctacagc
     1261 aggtttattt accggaatat ttctcgattc tttattgttt cttttaggaa taaacaaaga
     1321 agctgtttca tcttttattt caaaatcttt catttcaatc ccagctagtt ctgaaatagt
     1381 aattcctgtt tgaagtagaa gttctatcat agcgtagctt ctgacatcat tttttgcaga
     1441 atctctcaaa gctctgtact ccatttttga aagtattctt ggtgcacctg tttcaaattt
     1501 tgggtgtttt aattgagaag aaatgtcttc ttttagatac ccttcttttg ctaaatattt
     1561 aataaaagtt ctagcggcat ttgtttttct tgagattgat tttggagtgt agtttttctc
     1621 acctaaatcc ttcataaacg caactattgg ttcaattgaa attttttcaa cttcttcaat
     1681 ttttttagat gacaaaaaag ataacagttg ttctatgtct ttgttatagg caacaactgt
     1741 agctcccgac tttccttcgg actttaattc ttcgataaat tttttctgcg ctatatctag
     1801 tttcataata ttaatttttt tgagcagatg tattttctaa gggggctttt tatctaaaga
     1861 aaatattaag taaattctat gggcctatct taccaaaacc gttgaaaact gtcaattaat
     1921 taactattat aggtttaatg tcctcaataa gcagattaaa atatatttta ggtatcgtat
     1981 tttttacagt gttatcggta aacaccatca aatcagcact aaatgtttta aaaagtaagg
     2041 ataggttgga tcaggttaac aatgaagtag ccgtgttgga agatgaaaag aagaagatag
     2101 aagaagaaat agagtttaaa aaaacagatg aatatattga ggcgaaggca aggaacgatt
     2161 tgaatcttat taagccgggg gagaaggtct atgttgtgaa aaccactgaa aaggacaccg
     2221 caggaaatgt tttatctgaa acagacactg ctgcttcaga aaaaggtttg gataaaggta
     2281 atgaggaaaa aaagaatgaa aattggtatt cctggtacag acttttcttt gataattaag
     2341 gattttgaca cttctgggtt tatctgttag attatcaaag aattaaaaaa acatcgcggg
     2401 atggaggaat ggcacctcac cgggctcata atccgggggt cctggttcga gtccaggtcc
     2461 cgcaaccaaa gaaaaatatc aagcacttgt aaaagtgctt tttttatttg tgtttaatct
     2521 tgtataatac gggtgctgtt tgttgaatct tgccaatgta gctcaggggt tagagcgggc
     2581 gtttcataag cgctaggtcg gtggttcaag tccacccatt ggcaccaata aattgaagat
     2641 tttgaacccg gtatttttat gaaaaaatcc ttcttttaat gtatactctt aaagctatgg
     2701 aagaacaaaa agaaaaaaat caaaaagata ataaaaaacn nnnnnnnnnn nnnnnnnnnn
     2761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2821 nnnnnnnnnn nnnnnnnnnt tactaatgtt tggacttcct ttcttagtcc ttttttactt
     2881 tttaagacaa atgaaaggcc agaacggaga tattatgtct tttggaaaat cacgcgcaaa
     2941 gctctttgta aaaggtcagc aaaaaattac atttaatgat gttgccggtt gtgaggaagc
     3001 caaaagagaa atggccgaga tagttgattt tcttaaattt cctacaaaat atagaaaact
     3061 tggggcgagg attccaaaag gtgttttact ggtaggtcct tccggggttg ggaagacttt
     3121 attagccaga gcaatagccg gagaggcgga agttcctttt tttagtgttg ccggtagcga
     3181 gttcatggag atgttagttg gtgtaggatc atctagagtg agagatcttt ttgcaatggc
     3241 aagagaaaaa caacccagct taatttttat tgacgaaata gatgccatcg gacgacaaag
     3301 agggatggga attggaggag gccacgacga gagagaacaa accctaaatc aaattttaac
     3361 cgaaatggac ggttttgatg ttagaacaag cgttatcgtg ttggcagcaa caaacagacc
     3421 ggatatgctg gattctgctt tggtcagacc cggaaggttt gatagaagaa tctcaatccc
     3481 tcttcctgat ttaaaagaca gagaagagat aataaaaatt catatgagag ggaagcctat
     3541 aaatgccgat gttagtgtgg aaaaactggc taaaaagaca gtgggtttta gcggggctga
     3601 tattgaaaac atgttaaacg aagctgcaat tcttaccgcc agggaaaata gagaatccat
     3661 caccgacaaa gatttagatg aagcttcttt aaaagttact attggttcag aaagaaaaac
     3721 acttcaaagt gatgaagaaa aaagaatgac cgcttatcat gaagccggtc atgctcttgt
     3781 cgcagttaat gtaaaagata tggatccggt tcagagagtt tctattgttg ccagaggtca
     3841 aacacttggt cacacagatc ttccacctga aagagacagg taccaactaa caaaaacaag
     3901 aatcgagtca ttgataacca ctatgctcgg gggaagagcc gccgaagaaa tagtttttgg
     3961 cgaacttact gctggtgcgt ctgatgatat aaaaagatgc accgaacttg ccagaaaaat
     4021 ggtaaccgaa tatggtatga gttctttagg tccaatcttc tttgagggac gaagtgaatt
     4081 ctcatggctt gccgaagaat ttggggaagg ctcctcgatc agtgaagaaa tgtcctctaa
     4141 gattgataag gaaatttctg gtattgttga aaaatcatac caagaagcaa agaaaattct
     4201 aaaagaaaat aaggaaaaat tagataaggt cgttgcaaaa ttattagaag tagaaactat
     4261 cggaggggac gagtttaagg aacttgtttc ttaattaaaa taatagtcac atgttgatag
     4321 attcccattg tcatctaact cacgagttct acgaaaataa tcttgaagag ttgattaaag
     4381 attctatcga attaggaatt tcaaatttta ttaccatagg tacttcagta ttagaaaatc
     4441 aaaaagtaat tcaagtatcc gaaaattacg aaaatgtttt ttgtactgtt ggtatatatc
     4501 cgcacgaaaa tcaggaatta acatttgata ttttaaaaaa atcactggag gaaaatatta
     4561 actcaagtaa aaaaattgtt ggtataggag agtgcggcat agatataaca aacagggaca
     4621 gtcaaagaaa gttggaggat caaaagtctt tattcgaaat gcaactttct ttagcagtaa
     4681 aatacggtat tcccgttacg gtacacaata gaaatggaga cgaaataatt ctaaatattt
     4741 tgaaaaagta ttcaagtaat aatctaaggg gtgtgattca ttgtttcgat tcaaactggt
     4801 cttttgcaaa aaacgtgctg gatctcggtt tttatatttc tttttcaaat atggttactt
     4861 atcctaaaaa agatgcactt ttagaagttg ttaaaaatgt acctaaagat agatttttaa
     4921 tcgaaactga ctccccgtat cttccacccc aatctatgcg tggtgagata aattatccta
     4981 agtatgttaa aatcgttgca gagaaagtcg cacagataaa gcaaaaaaca tttaatgaag
     5041 tctcggagct ttcttttaag aatacctgct ctctttttaa tatatgttgt caaaatttgt
     5101 agaaaaacac gatcgactgg ttagtagaat attggagata atgctgggta ttttaacctg
     5161 gagcctgctt ctatcacctg tatggctagg gatattattt cctcaagcag ttgtttacgt
     5221 tttgacattg cttacagttt attggagtta tatggctttc acccatacct tcggtttatt
     5281 tatggggtat aaacaatata gggaggaaat ggctacagat tggtacctaa aatgtcaaaa
     5341 gttagatttt tccagtcttc cagaaccaga aacacgtcct aaatatttat ctgatatcag
     5401 acatatgatt ttaattcccg ttgtaaatga gcccgaggct gttttaagag attctatatt
     5461 tagtattttt aatcagactc ttcctacatc tcaaatttgt atggttttta ctgtagagca
     5521 aaaatattct gaagaagtta tagagcgaat taaaaatatt attggtgata aggataaaga
     5581 tctacatagt ttaatgtttt ttatccatcc tgcaggaata ccgggtgaag ccattggcga
     5641 cggtggtgct aaccgtgctt ggggttcttc tcacgcggta gaggagttga aaaaaagagg
     5701 tgataattta aggaatttta tttttagtaa tatagactcc gatcatgttt taaataaaca
     5761 gtatctttca agactgacat atctttattt gactacagac aggagagaca accattttta
     5821 ttcttctgcc gttcctttat ttgataacaa tctttggaga gttcccctta tgatgaggtt
     5881 agaagctaat tcggttactt taggcgtcat ctcagactgg gcagtttcaa aaggaaaaat
     5941 aagaaagtgt ttttcagctt tttcagtttc tttgcaaact ctaatagatg ccaatttttt
     6001 tgatgtggcg ttaggggcag acgatactat attttattgg agagcttttt ttgcacgaaa
     6061 cggagatttt gacggtgtgg gtcattttat accttactca gctgatgccg ttgagggtaa
     6121 gaactttaga gactctcaca ggagcttata caaacaactt ttaagatggg gttggggagc
     6181 cgttgacttt cctttatcaa ttaaaacatt tttgaaagac cacaacattg agtttgcaaa
     6241 aaaattcaga tgggtgagaa gacacataaa agagagggtt attttgacaa acatagtttt
     6301 tctaatcact tttggttttg ccattgttac actggtgaac ccatatgtaa aacaatcaaa
     6361 ttttgcctac agtctcccta aaattatgag tttcatacta acattaacaa tgtcgtttat
     6421 gattcccggc gtatatttca gaaaaaagtt ttcagttgcc cctccaaaag aatggggccg
     6481 atttagaaaa tttttaactt attttgaagc accgttaatt actattaatc ttttgactta
     6541 ttctttcttt ccgacacttc atgctcaaac catgttgatt ttcgggaaga gatttaagga
     6601 tttgtaccat acacaaaaga ttaggtagat atgaaattaa ataagaggat tgagaaaaca
     6661 actgaatata ttttagtgaa gcttaaagat aaaaatctct caaattttct tgttaatacc
     6721 atcatagata tttttctatt tatatacatt cgttcaaggt ttcagtattt aaataacgat
     6781 ataccgttgt ggtacacaaa gatttggggc gatgctcaat tagctccaaa atttaacctt
     6841 tatctaatac ctttggtggc aatggcagtc aatgttgtag ctctaatatt tacacttctt
     6901 aatagatact accttagata tttggaggat gttctttggt attgcgtatt attctttaac
     6961 atttttatag tgggttctgt tttaagaata atcagaatat cctcaatacc ttttagctct
     7021 tttataaatc cactatatgt aaaattgctc gttcctatgt cattttcatt aatttttatg
     7081 cttataacga tgccctactt tatagatttc gcaaaaaaga aaaagttaat aacaaacccg
     7141 caagtgcatt cgcatcccgg catgatatta agagcacctt ctgcaagggg cgggggtctc
     7201 ctttactcaa taattttaat gatttcagca atcatattta taggattcag taaacagttt
     7261 tcaggtttat acatatctct aataatgatt tctgttttaa gttttgtaga tgattatcaa
     7321 aacacacatc ctaaatctag ctatagattt cttgaaaacc cagttcttag gctatttctg
     7381 ttatttttaa gtgtaatccc tgtcgttttt tcaggagtta tgattcatac agtaagtaat
     7441 ccttttggta gttttgttaa attagatgtt attgaaataa atatttttaa cagttccatc
     7501 cctctgatat ctctccttgt tacttctctt tggatagttt ggttaatgaa tgttctttct
     7561 tggtcaaacg gtgttgacgg ccagtttcca ggtatagtag gaatagcgtc tctcttagtt
     7621 gccttgttgg cactgagatt tgaggaactt acagattctc attatcaaat tgctgttttt
     7681 tcagcaatta gtgcaggcgc agcttttggc tcggtaagat acaattggca cccctcaaaa
     7741 attatgtggg gctttggagc aatgtcagct ggtttggtga tagcgagttt ggcaatatta
     7801 gcccaggcta aaattgctgt atccgtatta atactactaa tacctttttt ggatgcgtta
     7861 ttcactgtta gtaggaggat tcttcatggt aaaaacccgt tcaaaggaga taggggacac
     7921 ttacatcatt tactgttaga taagggttgg agtgttagca aggtggcaat tttctactgg
     7981 ttagcaactg ctttatttgg tctagttggt ttgttttctc ctgaaagaat agttttaaaa
     8041 ctaacctttt taataatggg tgtggttgct ttcggtctca tagttttaaa tatcagttta
     8101 aacacaagaa agaaagagac tcctcccaca gtcttgtaat agttagttac tcatacgtct
     8161 tctaaatatt taattttcat ttaatgttcg ttggaaagaa tattgaaagt gtgccaaaac
     8221 aaaaaatgag gatacttttg gtttcaagtg acaataaatt aattgaaaaa ttaaacctca
     8281 atttgaggga ttgttacatt gtcgatacag ctcgaggggc ttcgcaagct atcaatctgt
     8341 ctgaaacgaa ttattacgat tctattgttg ttgatactta tttagatgat atagagggct
     8401 tggagttatg taaaatgata agagattatg atgttgattc tcctaccgcg tatttatcaa
     8461 ataaagaaga tcgttccgat agagttatgt gcttagatgc aggtattgat gtgataatac
     8521 caagatacgc aggtgtggag gaaatacttg gacagattca cgcactagcg aggagaaacg
     8581 gcaatcacaa aaatggtaga aatgttttga agatcaggaa catttcactc gatatgaaaa
     8641 atagaatttt ttatatagat ggaaggtgcc ttcatttgag aaggaaggag tttgatctta
     8701 tggagtacct tatgataaat aaaggaagag ctgtttcaaa agaagaatta ctggagcatg
     8761 tttgggagaa aggattggat gttatgagca atactgttga ggtacatatt agaagtcttc
     8821 ggaaaaattt tgaatctatg ttaggtgaaa aaataattag aactaacaga ggcttcggat
     8881 atgaaattga gacttgagaa aaagttataa aacccctatt atcaacataa tgatgagaaa
     8941 aatttaccac atattaagaa cagcccgacc aaggcagtgg ctaaagaatg ccagcgtttt
     9001 tgcagctcca ttcttcgctg gaacattgtt ggatggtgag gttttttcat ggtctttgaa
     9061 agcttttgtg gcattttgcg caatatcttc cggggcgtat tttcttaacg atttagttga
     9121 tgctccaagg gacagactac accctgtaaa gaaaaacaga gccatagctt cgggagaaat
     9181 atctccttta ttagcgataa ttatttcttt agctttaatt tctttctcaa t
//
LOCUS       DAOTFA010000013         8486 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate AMR_MDS_3115
            NODE_5908_length_8486_cov_6.422963, whole genome shotgun sequence.
ACCESSION   DAOTFA010000013 DAOTFA010000000
VERSION     DAOTFA010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868139
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (wastewater metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8486)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8486)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:36:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 536
            CDSs (total)                      :: 521
            Genes (coding)                    :: 520
            CDSs (with protein)               :: 520
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8486
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5908_length_8486_cov_6.422963"
                     /isolate="AMR_MDS_3115"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            176..1489
                     /locus_tag="P5014_00965"
     CDS             176..1489
                     /locus_tag="P5014_00965"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRY56712.1"
                     /translation="MKNKITTAILLLLIPLFLTACTLSDIPVVGKYFGGGAGNPGKPG
                     VPVSLNMWGLWETPDIMDVIIKNYQTKENLVTVNYEDRSINNPNGYKDSIFTRIKQGD
                     LPDIVMVHNSWVPFLKDYLEPAPSDVIAVADYSQRFYPVATESSVVDGKVYAVPAHYD
                     GLVLVYNKDHFDEIDQVSPPTAWEEFRRVALALTVRTDKEGLVRAGAAIGAANNIDFF
                     SDIVGLMFAQAGVSVPSGVDSRAAQDALSFYTLFLKSDGVWSESFPEASKAFSAGKVS
                     MIFVPTWNLLDIVRANPGMNIGVAPVPQAIAEKPVSWGSFWMYAVPKSSSNSKAAWKF
                     INFVSQDDQQLALFNEASKYRPYGAPFSSVNLSSQAGQSASSQYIKPVLNTAPFAKSN
                     IFAGRSGNNTEVEALKTAVNAVLSGEVTSEQALKTCKETITGTQQ"
     gene            1535..2590
                     /gene="tsaD"
                     /locus_tag="P5014_00970"
     CDS             1535..2590
                     /gene="tsaD"
                     /locus_tag="P5014_00970"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HRY56713.1"
                     /translation="MLIFGIETSCDETAAAIVEDGVNEICSVVSSSKEFHEKTGGIVP
                     EVAARKQVESIVPVISECFEKYKEIKGYESIEHCYTDIDAVAVTIGPGLLASLVVGVE
                     AAKSLALFLNKPLIPVNHLIGHLYGNYLVEEGVTKNIAFPAVALLVSGGHTELILIKN
                     HGDFEYIGGTLDDAVGEAYDKVARLLGLSQYLGGALLSKKAQECKVNKIKGKLPRPMI
                     DQDNFDFSFSGLKTATRRLIENEKIPTEVIAREFEDAVVEVLTKKTLNAVKKFSVKSL
                     LMGGGVSANLHLRETLGRECEKVNVKFYAPPLRLCTDNAVYIASAAYFMYANEQYDFE
                     KIKFEDIQPNPSLGIID"
     gene            complement(2611..3858)
                     /locus_tag="P5014_00975"
     CDS             complement(2611..3858)
                     /locus_tag="P5014_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020254374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HRY56714.1"
                     /translation="MRYSKIIGKTKKDAPKDEESVSAQLLLRGGFVHKEIAGVYTYLP
                     LGLRVLNKIIQIVREEMIAIDGQEMSLTALQNPEVWKKSGRYDDDVVDIWFKTKFKNN
                     TETCLAITHEEPLTNLMIQQINSYKDLPLYAFQFQTKFRNEVRSKGGLLRAREFLMKD
                     LYSFDKTKEDHEKYYERAKQAYIKIWKRLGIGDDTYVTFASGGSFSKFSHEFQTVCKT
                     GEDTIYLDKKKKIAVNKEVFNDEVLKMLDINKNDLEEVKAIEVGNIFPLGTKYSDALG
                     LYYTDEEGQKKPVIMGSYGIGMGRVMATIVELKHDDKGIIWPENIAPYKVMLIGLNLE
                     DEKVKEKAEKLYEKIISMGIEVLYDDRKEVTAGQKFADCDLLGIPYRAVISQKTENRV
                     ELKKRAEQNTKDVSPEELLYLLK"
     gene            complement(3887..4474)
                     /locus_tag="P5014_00980"
     CDS             complement(3887..4474)
                     /locus_tag="P5014_00980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56715.1"
                     /translation="MSESIKAIGGINENSLNEYEKFIRTFIPENLTQEQLPTFLSEKR
                     SELRVKYGMPDRQPVLDGKETLEEYHEKLLLLAGEKEIGVASDFEEYTQKHHLPGGAN
                     ACFGDDTDYKIHVKKDFNPNDVPNVKSLEHELVHALQYKNEPGMPIEQKEFEAYLVAD
                     GLDDIFKNPNLRDIFFSLIISSVDGYKKSAISTEK"
     assembly_gap    4567..4666
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4717..6978
                     /locus_tag="P5014_00985"
     CDS             4717..6978
                     /locus_tag="P5014_00985"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HRY56716.1"
                     /translation="MEKKYDHKIQEPNIQQKWEDSGVFKGKIESEKKPFTIVLPPPNA
                     NGKLHTGHVLMLAIEDILIRWKKMSGCSTLWLPGTDHAGFETQVVFEKSLKEKGKSRF
                     DFDRDSLYREIWKFVEENKGVIENQMKRMGPGLDWSRYTYTLDESVVKTVNSTFQKML
                     SEGLIYRDNYIVNFCPKCGTTFADLEIKHKDKVSSLYYIKYGPLVVATTRPETKFGDV
                     ALAVNPDDERYQQYIGKEIEYEDPLGKFSIPVIGDKYVDMTFGTGVLKITPAHDKNDY
                     EIGLKHGLPVVSVIGFDGKMSENTGKYAGMDVSVAREEVVKDLQEMGMLEKIDTNYAN
                     SVTVCYKGGHEIEPTVLPNWFIKVESLKKPALDAVLNGDVKIYPKWRQITYTKWMEEM
                     RDWPISRQIVWGIRIPIWYDVDENPNLQVTFIARGGERISGRVEDLLKDYSFDEIKNG
                     LQNLIAPKDAKFITELEVKSHKESESGKLLQETDTFDTWFSSGHWPLATLRYPDSDDF
                     KYYYPTSVMETGWEIIRFWVSRMIMFGIYLTGKPPFKDVYLHGLVRALDGKKMSKSLG
                     NVIDPLDYIEEYGADALRMGLISGTANGKDFAFPKDKVIAYRNFANKLWNMARFMLMM
                     MGDFEEGTVFDYSKIDHSSLSSTDLELLESLKKLISNVNANLERYRFSDASDLIYQFM
                     WHEIADKYIEHVKGLSGGEKITSLSVLRYVYVTGLKLLHPFMPFVTESIWENILKFEG
                     EEDLLLSSNWPQN"
     gene            complement(7073..7747)
                     /locus_tag="P5014_00990"
     CDS             complement(7073..7747)
                     /locus_tag="P5014_00990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HRY56717.1"
                     /translation="MQLQAKKRETLGKKTKYIRRGGEVPAVVFGKGMDSVNISMDYNT
                     FEKTFRQAGETDLLDVVADRDKYKVLIKDVQYDPVTTRISHASLYKPDLTVKTEAQVP
                     VEIIGEDTNELIKSGAALALQLLQEITVEALPEDIPHHFVIDVSALNEIGQGVTISQL
                     EFDKDKVTIPDIDPEEMIVRIDEIVIQEEEPEVAPISEEEAIAGLEATAEKPATEEEE
                     KSEEKK"
     gene            complement(7839..>8486)
                     /locus_tag="P5014_00995"
     CDS             complement(7839..>8486)
                     /locus_tag="P5014_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56718.1"
                     /translation="GKIFKSEVALPNFSQESNIYKGIRSLGKEGSLFTFLDDEGYKTY
                     DIATSQVKETYKLPSAKIVYPYSGYVYSISEDILSRGSVENNELKQGVWGQNQNFRDA
                     VSISIAYSVYVLTNQGALVNYSGGVETDFTVSGLDKPFLNPIKVIADLDFENIYIADR
                     GNNRVVILNEDGELVKQFKNDDDSLWYDIKGISVSSDEKYLFVLDTSKIYKVTLE"
BASE COUNT         2492 a         1421 c         1586 g         2887 t
ORIGIN      
        1 taaattcaag ttattttcat gtttttttaa tttcgttact acagatagta ttgctgggta
       61 gttttgacca ttatcttttt acaatgcacc aaaccctttt attattttgg attatttttg
      121 gcttaagttt acaatagtat tggattttta atttagtgta tatataatta tgaagatgaa
      181 aaataaaatt actactgcca tattattatt gcttattcca ttgtttctaa ccgcttgcac
      241 actttctgat attcctgttg ttggtaaata ttttggcgga ggtgctggta atcctgggaa
      301 acccggagtt cctgtaagtt taaatatgtg ggggttgtgg gaaacaccgg atataatgga
      361 tgtaataatt aagaattatc aaactaaaga gaatttagtt acggtaaatt atgaagacag
      421 gtcaataaac aaccctaacg gatataaaga cagtattttt acaagaatta agcaaggtga
      481 tttaccagac atagtaatgg tgcataatag ctgggtacct tttttaaaag attatctgga
      541 accagctccc tcggatgtta tagctgttgc agattactca caaaggtttt atccggttgc
      601 cacagagtct tctgttgttg acggaaaggt ttacgctgtt ccagctcatt atgatggttt
      661 agttttggtt tacaataaag atcattttga cgaaatagac caggtttctc cacctactgc
      721 ttgggaagaa tttaggagag tagccttagc tttaaccgtt aggacagata aggagggtct
      781 cgttagggca ggcgctgcaa taggagcagc aaataatatt gatttctttt cggatattgt
      841 gggattaatg tttgcacagg caggtgtttc ggttccttcg ggtgtagata gcagagcggc
      901 tcaagacgcg ttatcatttt acacattgtt cttaaaatca gacggtgttt ggagcgagtc
      961 ttttccggaa gcatctaaag ctttctctgc cggtaaagta tctatgatat ttgttcctac
     1021 ttggaacctt ttagatattg ttagagcaaa tcccgggatg aacatcggag ttgctccagt
     1081 tcctcaagca attgctgaaa aacctgtttc atggggaagt ttttggatgt atgctgttcc
     1141 aaaatccagc tctaattcaa aagctgcgtg gaaatttatt aactttgttt ctcaagatga
     1201 ccagcagttg gctttattta atgaggcttc taaatacaga ccttacggag ctcctttttc
     1261 atcggtaaat ttgtcgtctc aagcaggtca gagtgcttct tcgcaatata taaaaccggt
     1321 tttaaataca gcaccttttg caaaaagtaa tatttttgca ggaagatcag gtaacaatac
     1381 tgaagtagag gctttaaaaa cagcagtaaa cgcagtttta tccggcgagg ttacctcaga
     1441 acaagcttta aaaacttgta aagaaacaat tacaggtaca cagcagtaaa tattaaatta
     1501 ttgaacctat gttttttgct ggtatatttc cattgtgtta atttttggta tagaaacatc
     1561 ttgtgatgaa acagctgctg ctatcgttga ggatggcgta aatgaaattt gcagtgtagt
     1621 ttcatcttca aaagaatttc atgaaaaaac aggcggaatt gtacccgaag tagcagcgag
     1681 aaagcaggta gaatcaatcg ttcccgtaat atcggagtgt tttgaaaaat ataaagagat
     1741 aaaaggatac gagagtatag agcattgtta tacggatatt gatgctgtgg cagtaaccat
     1801 tggtccggga cttttagcgt cattggtagt tggtgtagag gctgccaaat cattggcgtt
     1861 gtttttgaat aaaccattaa tccctgtgaa tcacttaatt ggacatctct acgggaatta
     1921 tttagtagag gaaggggtta caaaaaatat tgcatttcct gcagttgcgt tgcttgtgag
     1981 cggaggtcat acggaattaa ttcttataaa aaatcacggg gattttgaat atatcggagg
     2041 tacgttggat gatgctgtag gggaagctta cgataaggtt gccagacttt tgggtttgtc
     2101 acagtacttg ggaggtgccc ttctatctaa aaaggctcaa gaatgcaagg taaataaaat
     2161 aaaaggaaaa ttaccaagac ctatgattga tcaggataat tttgattttt cattttcagg
     2221 tttaaaaact gctacccgaa gattaatcga aaatgaaaaa atccctaccg aagttattgc
     2281 tagggagttt gaagatgctg ttgtagaagt tttgactaaa aaaactctaa atgcggttaa
     2341 aaaatttagt gtgaagtcac tgctgatggg gggcggggtg tccgctaacc tacatttacg
     2401 agaaacattg ggaagggagt gtgagaaggt taacgtgaag ttctacgccc cgccccttcg
     2461 actttgtacc gacaatgctg tgtacattgc cagcgcagcg tactttatgt acgcaaacga
     2521 gcaatatgat tttgaaaaaa taaagtttga agatattcaa ccaaacccat cattgggaat
     2581 tattgattga agtttagttt gtaccaggat ctattttaga agataaagaa gttcctcagg
     2641 agatacgtct ttagtatttt gttcagctct tttttttagt tcaactctgt tttctgtttt
     2701 ttgtgagatt accgctctgt atggtatccc tagaagatca caatcagcaa atttttgtcc
     2761 tgcggtaact tcttttctgt catcgtataa aacctctatt cccatagaga ttattttttc
     2821 ataaagtttt tctgcttttt cttttacttt ttcatcttcc aaattgagac ctataagcat
     2881 tactttgtaa ggagcaatgt tttcaggcca gatgattcct ttatcgtcat gttttagttc
     2941 tactattgtt gccatcactc ttcccatacc aattccgtaa cttcccatta tcacaggttt
     3001 tttttgacct tcttcgtctg tgtaatataa acctaaagca tctgaatatt tagttcccaa
     3061 aggaaaaata tttcctactt caattgcttt tacttcttcc aaatcgtttt tattaatatc
     3121 taacattttt aaaacttcgt cattaaaaac ctctttatta acggcaattt ttttcttttt
     3181 gtctaagtaa atagtatctt caccagtctt acagacagtt tgaaactcgt ggctgaattt
     3241 gctgaaactt ccaccactgg caaaagtaac ataagtatca tctccgatcc ccagtctctt
     3301 ccagattttt atgtaagctt gctttgctct ttcgtaatat ttttcgtggt cttcttttgt
     3361 tttatcaaaa gaataaaggt ctttcattaa aaactcacga gctcttaata gccccccttt
     3421 tgacctcact tcatttctaa attttgtttg aaattggaag gcatataaag gcaagtcttt
     3481 atacgagttt atttgttgaa tcattaagtt agttaatggt tcctcgtgtg taatagctaa
     3541 acatgtctca gtattgtttt taaattttgt tttaaaccaa atatcaacaa catcatcgtc
     3601 gtacctccct gatttcttcc aaacttccgg attttgaaga gctgttaacg acatttcctg
     3661 cccatcaata gctatcattt cttccctaac tatttgtatt attttattta aaacccttaa
     3721 gcctaaaggt aggtatgtat agacaccggc tatttcttta tgaacaaacc cacctctaag
     3781 taagagttgg gcgctgacgc tttcttcatc tttcggggca tctttttttg tctttcctat
     3841 aatttttgaa tatctcatat gtattgattt tatcacatta aaatgctcac ttttctgttg
     3901 atattgcgga ttttttataa ccatccacag aagatattat caagctaaaa aatatatctc
     3961 ttagatttgg atttttaaaa atgtcatcaa gtccatcagc aactaaataa gcttcaaact
     4021 ctttctgctc aattggcata ccaggttcgt ttttatactg taacgcgtgt actaattcat
     4081 gttccagcga ctttacatta gggacgtcgt tcggattaaa gtctttttta acgtgtattt
     4141 tatagtcggt atcgtctcca aagcaggcat tagcccctcc cggtaggtgg tgtttttggg
     4201 tgtattcctc gaaatcactt gctacaccaa tttctttttc tccagccaag agaagtagct
     4261 tctcgtgata ttcttctagt gtttctttcc cgtccaagac aggctgcctg tcaggcatgc
     4321 catacttaac ccttaattcc gagcgttttt cagatagaaa tgttggtaat tgttcttgag
     4381 tcaaattttc cggaatgaat gttcttataa atttttcata ctcatttaat gaattttcgt
     4441 ttattcctcc tatggctttg atactttctg acatatttat attctataat aataaccaca
     4501 cggtgttaat taaatgaacg ttgttctatc tttatatttc tgataacatc gacctgttct
     4561 tatggannnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnaaat gaacgttgtt
     4681 ctatctttat atttctgata acatcgacct gttcttatgg aaaagaaata cgaccataaa
     4741 attcaagaac cgaacattca gcaaaaatgg gaagattctg gtgtatttaa aggtaagata
     4801 gagtctgaaa aaaagccttt cacaattgtt cttcccccac ccaatgcaaa tggtaaatta
     4861 catacaggtc atgttttaat gctggcaatt gaagatattt taattcgttg gaaaaagatg
     4921 tcaggttgct ctactctgtg gcttccgggc actgaccacg cgggttttga aacccaagta
     4981 gtttttgaaa aatctttaaa agaaaaagga aaatcgagat ttgattttga tagggactct
     5041 ttgtacagag aaatatggaa atttgttgag gagaacaaag gcgtcattga aaatcaaatg
     5101 aaaagaatgg gtccggggtt ggattggtct cgttatactt acactcttga tgaaagtgtt
     5161 gtaaaaactg taaattcaac ttttcaaaaa atgttatccg aaggtttaat ttatagagac
     5221 aactatattg tgaatttttg tccaaaatgc ggaactactt ttgcggatct tgaaattaaa
     5281 cataaagata aggtaagtag tttgtattac ataaaatatg gaccattggt ggttgctact
     5341 acaaggcccg agactaagtt tggggatgtg gctttagctg taaatcctga tgatgaaaga
     5401 tatcagcaat atattggaaa agaaattgaa tacgaagatc cgttgggtaa attttcaatt
     5461 cctgttattg gcgataagta tgttgatatg acttttggta ccggtgtttt aaaaattacc
     5521 ccagcgcatg ataaaaatga ctatgaaatt ggtttaaagc acgggcttcc tgtagttagt
     5581 gtaataggtt ttgacggaaa gatgtctgaa aataccggta agtatgctgg aatggatgtt
     5641 tctgtggcgc gtgaggaagt tgtaaaagat cttcaggaaa tgggcatgct tgaaaaaatt
     5701 gatacgaatt atgcaaattc tgtaacagtt tgctacaaag gaggtcatga gatagaaccc
     5761 acagtacttc ctaattggtt tattaaggtt gaatctttaa aaaagccagc actggatgcg
     5821 gttttgaacg gtgatgtaaa gatttaccct aagtggcgtc aaattactta tacaaagtgg
     5881 atggaagaaa tgcgagattg gcctatatca aggcaaattg tttgggggat tagaattcct
     5941 atttggtatg atgtcgacga aaaccccaac ctccaagtta cgtttattgc tcggggcggg
     6001 gaaagaatct cagggcgtgt agaggatttg ttgaaagatt actcgtttga tgagattaaa
     6061 aatggtctac aaaatcttat tgcaccgaaa gacgctaaat ttataaccga gctcgaggtt
     6121 aaatctcata aagaatctga atccggaaaa ttacttcaag aaactgacac tttcgatact
     6181 tggttttctt caggtcattg gcctttagct actttaagat atccggattc tgacgatttt
     6241 aaatattatt atcctacttc cgtgatggaa acaggctggg aaattatcag gttttgggtt
     6301 tcaagaatga ttatgtttgg aatttatctt acaggcaaac ctccgtttaa agatgtttat
     6361 cttcatggtt tagtcagggc tttagacggt aaaaaaatga gtaaatcttt aggaaatgtt
     6421 atcgaccctc tggattatat tgaagagtac ggagctgatg cattacgaat ggggttgata
     6481 agtggtactg ccaacggaaa agattttgca tttccgaaag ataaagtaat tgcttatagg
     6541 aattttgcaa ataaattatg gaatatggca aggtttatgc taatgatgat gggtgatttt
     6601 gaggaaggca ctgtatttga ttatagtaaa attgatcaca gctctttaag ttccacggat
     6661 cttgagttac tcgagagttt aaaaaaatta atctcaaatg ttaatgctaa tctcgaaaga
     6721 tacagatttt ctgacgcttc tgatcttata tatcagttta tgtggcacga aattgctgat
     6781 aagtatattg aacacgttaa gggattatca ggtggtgaaa aaatcacatc cctatcagtt
     6841 ttaagatacg tatacgttac tggattaaaa cttttgcacc cgtttatgcc gtttgtcact
     6901 gaatcaattt gggaaaatat tctaaaattt gaaggtgagg aagatttact attatcttct
     6961 aattggcctc agaattagag agtttttcta caacattttt atatcaaacg cttggaataa
     7021 aatacttgta aatctagtac ggaagttttg attatactta aaagtatagt tcttactttt
     7081 tctcttctga tttttcttct tcctctgttg ctggtttttc tgcagtagct tcaagacccg
     7141 ctatagcttc ctcttcagat ataggggcta cttcaggttc ttcttcttga attacaatct
     7201 catcaattct tacaatcatt tcttcaggat ctatgtccgg gatggtaact ttatctttat
     7261 caaactcaag ttgagagata gttaccccct gcccgatttc gtttaacgca gaaacatcaa
     7321 taacaaaatg gtggggaata tcttcaggaa gagcctctac agtaatttct tgcagtaact
     7381 ggagtgctaa agcagctcca gatttaataa gttcgtttgt gtcttcacca ataatctcaa
     7441 caggaacttg agcttccgtt ttaactgtaa gatcgggttt gtataaagaa gcatgtgaaa
     7501 ttctggtagt aactggatcg tactggacgt cttttataag aactttgtat ttatctctat
     7561 cagccactac atctaaaagg tctgtctctc ctgcttgtct aaaggttttt tcaaatgtgt
     7621 tgtaatccat ggatatattt actgaatcca ttcccttacc aaatacaaca gcaggaactt
     7681 ctccgcctct tcttatgtat ttagtttttt ttccaagagt ttctctcttt ttagcttgta
     7741 attgcatata ttcttttagg gcattggtat tctacttaca aaaagccctc aggtcaacat
     7801 cctgatattt taacagaaaa aactaataag ttaaggtttt actcaagagt tactttgtat
     7861 atcttgctcg tatccagtac aaacaagtat ttctcgtcag aagaaacaga aattcctttt
     7921 atgtcgtacc atagagaatc gtcatcgttt ttaaattgct ttaccagctc tccatcttcg
     7981 ttaaggatga cgactctatt attgcctctg tcggcaatat aaatgttttc aaaatctaag
     8041 tccgcaatta ctttaatcgg gtttaaaaaa ggtttatcca aacctgaaac tgtaaaatcg
     8101 gtctcaactc ctcccgaata atttactaag gctccttgat ttgttaaaac atatacacta
     8161 tacgctattg atattgaaac cgcgtccctg aagttttgat tctggcccca aaccccttgt
     8221 tttaactcgt tgttttccac agacccccta cttaaaatat cctccgatat ggaataaaca
     8281 tatcccgaat aagggtacac aatttttgcc gatggtagtt tgtatgtttc ttttacttga
     8341 gaggtagcaa tatcataagt tttataacct tcgtcatcaa gaaatgtaaa taaactgcct
     8401 tcctttccta atgacctgat gcctttgtat atattagatt cttgggaaaa gtttggtaac
     8461 gctacttctg atttgaatat ttttcc
//
LOCUS       DAOTFA010000014         8153 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate AMR_MDS_3115
            NODE_6223_length_8153_cov_6.966411, whole genome shotgun sequence.
ACCESSION   DAOTFA010000014 DAOTFA010000000
VERSION     DAOTFA010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868139
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (wastewater metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 8153)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8153)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:36:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 536
            CDSs (total)                      :: 521
            Genes (coding)                    :: 520
            CDSs (with protein)               :: 520
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8153
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6223_length_8153_cov_6.966411"
                     /isolate="AMR_MDS_3115"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            23..2359
                     /locus_tag="P5014_01000"
     CDS             23..2359
                     /locus_tag="P5014_01000"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HRY56719.1"
                     /translation="MSRGRKKKFKLKINVKPDTVRSIVSLFFLALFLVTLISFFAPDY
                     EINSRIQHFLKLYFGKSSILVPFIFGSFGILFFDRIGERFKEARIFIGLFLMLFSLSA
                     VLHVFISEENSYDVALAGKGGGLIGYRISSTLSSGISIIGAVALIFIGILGSIFLIFN
                     LSLNNIADFLQKIGLADLFKKIFSKKERDEVESEIEVNTSSVLTVDDYKEDKKNTAFA
                     DVHSGDAREPAQASIFEVIQPQAEPLGDFKHISNGSVDTLAPVSLIKASPAIPSDRIW
                     DYPPENLLAEPPVRVVDTSELDKRIKIIKDTFKSFSIDVDIDMENVKVGSSVTQYALR
                     PKSVTNIAKIATLQGNIALALASPTGTVRIEAPIPGKSLIGIEVPNTKRTMVYFKSLI
                     ASPTMKNAPSKLSVALGEDVGGRVVTYDIGKMPHLLIAGTTGSGKSIFIHNLLFSMLF
                     RSTPQEVKFILIDPKRVELILYDGIPHLLTPVVTDMEKAPSVFRWAVEEMERRYKLFE
                     KARVKNIDTYNEKSGIQVMPYIVIVVDELGEIMIQDPAGVEKTIIRLAQMGRATGLHL
                     VLAVQRPSIEVITGLIKANIPCRVAFQVLNQIDSRVIIDQPGAEKLLGKGDMLFVPPD
                     TMKPVRLQGAFISEKETSDLVNFLKNQGVAPDYKEEVLSMPAVTSRGGVVRSSWGDDV
                     DDELFDKAVTIVVSAKKASSSLLQRKLSIGFARAAKLIDMMEERGIVGQEMGGSRGRE
                     VLIDDLPEDGLEIEKDSGFDAEDFAGENFPRNQTDFDE"
     gene            complement(2424..>2599)
                     /gene="prfB"
                     /locus_tag="P5014_01005"
     CDS             complement(2424..>2599)
                     /gene="prfB"
                     /locus_tag="P5014_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004608918.1"
                     /note="recognizes the termination signals UGA and UAA
                     during protein translation a specificity which is
                     dependent on amino acid residues residing in loops of the
                     L-shaped tRNA-like molecule of RF2; in some organisms
                     control of PrfB protein levels is maintained through a +1
                     ribosomal frameshifting mechanism; this protein is similar
                     to release factor 1; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HRY56720.1"
                     /translation="KKLKGEYKMPGWGNQIRNYVLHPYKLVKDLRTNLEITNPEGVLD
                     GNLEELIEAELKL"
     assembly_gap    2600..2699
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2700..3580)
                     /locus_tag="P5014_01010"
     CDS             complement(<2700..3580)
                     /locus_tag="P5014_01010"
                     /inference="COORDINATES: protein motif:HMM:NF015427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HRY56721.1"
                     /translation="MEEFQRRIEELKKTLKIDEKKQRLLDLKEKSKSEDVWKNWEEGN
                     KLSKEISDLEKEVEDFDMLYLYLENGDEVLIEKELKEFEFKTYLSGKHDDKGAILSIH
                     AGQGGTEAMDWTSMLLRMYLRYAEKRNWRVYTINEVPGEEAGIKTVSIEIEGKYAYGF
                     LKREAGVHRLVRQSPFNADNLRQTSFALVEVLPIIDDDEEIEIKNQDLEWDFFRAGGS
                     GGQNVNKVSTAVRLRHIPSGIVIECQEERYQGKNREKALRILKSKLYQMEQEKIQGEE
                     KKLKGEYKMPGWGNQIR"
     gene            complement(3639..4112)
                     /locus_tag="P5014_01015"
     CDS             complement(3639..4112)
                     /locus_tag="P5014_01015"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HRY56722.1"
                     /translation="MLKSKLSKLKTVLIIILFICTYLIFDYYQIKILKVVTSSMDPAI
                     KKGSLVLIRKDNVFKIGDIASYRTLQNKNVITHRITKISKLQDKYYFYFKGDANENPD
                     PSPISDSEIIGKYFFSIPFLGDMCNFMKNSKFLFILLSLFGGLFSGKLLKNFISS"
     gene            complement(4182..4766)
                     /locus_tag="P5014_01020"
     CDS             complement(4182..4766)
                     /locus_tag="P5014_01020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56723.1"
                     /translation="MKKKKLLYLIGSSLIVISLIAGITYAAFSDKAKVLGSSFSVGSA
                     NLMFLESLSMGTDPSNYKDELPGPTFTNIGPGWTQDYLLKLISMASSKISISTNAFYE
                     TANDPQELRSDIQVEIIKWYDTNQNGIVDAGEEGPVVGKKSIIKWKTEGFTLGDLYYG
                     QVMELILRFSAPTISELKQGASAVFDFEFNSIQS"
     gene            complement(4790..5470)
                     /gene="radC"
                     /locus_tag="P5014_01025"
     CDS             complement(4790..5470)
                     /gene="radC"
                     /locus_tag="P5014_01025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HRY56724.1"
                     /translation="MKIKDLQKEEKPREKMVEKGSEVLTDVELLAIIIRSGGKDTSAT
                     GLSRELILKFGNLKKVLSADIEQLKNFKNIDIAKATSIKAVEEISKRYLTPFKEDAIK
                     IKSPKDVYDLIRKEVINKEEEYLFLICLDSRNRLISQEVISKGTINETLIHPREIFKK
                     ALNKNAYSIILVHNHPSNEPDPSDEDIKVTKRIFRAGIDMGIPLTDHLIVTSNDFMSM
                     KAQNLISI"
     assembly_gap    5501..5600
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<5601..5968)
                     /gene="rpsI"
                     /locus_tag="P5014_01030"
     CDS             complement(<5601..5968)
                     /gene="rpsI"
                     /locus_tag="P5014_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009105733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HRY56725.1"
                     /translation="MAVEKKDKKEKKVVAKKTSTPKEQKVKEEKVVVDKEIEMEIEKE
                     ENEEVETKEEIKTEEKAVDTKPGKKGFFVGTGRRKTAIARVFLWDKKGEFTVNGKSIN
                     DYFESNVDKTKWEKPFHAIGV"
     gene            complement(5970..6401)
                     /gene="rplM"
                     /locus_tag="P5014_01035"
     CDS             complement(5970..6401)
                     /gene="rplM"
                     /locus_tag="P5014_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019477838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HRY56726.1"
                     /translation="MKTKPNKTTFLNIKEKKEAWYLIDAKDQTLGKVAEKISLILNGK
                     NKPEYTPSQNWGDKVVVINAEKIKVTGKKMKDKKYIHNTGFPHGLRTEKLESLLERKP
                     TEVLKKAVTGMLPKNKLKKERLNNLYIYAGENHPHQSQLSK"
     gene            complement(6382..6825)
                     /gene="rplQ"
                     /locus_tag="P5014_01040"
     CDS             complement(6382..6825)
                     /gene="rplQ"
                     /locus_tag="P5014_01040"
                     /inference="COORDINATES: protein motif:HMM:TIGR00059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HRY56727.1"
                     /translation="MRHRVVKKKLNRDMDHRKSLALNLLSQIIENGKINTTLAKAKWV
                     KPKIEKLITKAIKAHKSDDKIFKFNTVKALRKSLRSEEVIKKLMEEIAPKFLERAGGY
                     TKIIKTGNRDGDNSLTARIELVSSLEETKEKLKKKAVKKNEDKTK"
     gene            complement(6838..7758)
                     /locus_tag="P5014_01045"
     CDS             complement(6838..7758)
                     /locus_tag="P5014_01045"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF003519.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HRY56728.1"
                     /translation="MEVTKENLKINTIKEEGNMGVYSYEPLPTGFGYTLANALRRVLM
                     SSIKGSAATQVKIAGATHQFTTINGVKEDVVEITLNLKKLRFKSHSDEPVVVTISKKG
                     PGKITAKDIETTSDVEIMNKDQLIATLADSKSKLDMEMVIEQGIGYSPMEERQTSKMG
                     VIVLDALFSPIISANYEIVPTRFGGRTDLDKITFTIETDGSISPKDAIKEASKILKEY
                     YISFQEWKADVEEEVKEKPKSSVKMGDDVSVEELPLQTRTINALKKSKIMSLHELAKK
                     SNEELEDIKNIGEKSLQEIKDLLDKEGYRK"
     gene            complement(7801..>8153)
                     /locus_tag="P5014_01050"
     CDS             complement(7801..>8153)
                     /locus_tag="P5014_01050"
                     /inference="COORDINATES: protein motif:HMM:NF013633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HRY56729.1"
                     /translation="RGVSGNILMQKLESRFDNLVYRSGFAVSRAQARQNIRRGLFTVN
                     GKKVTIPSMEIKVGNIIKPESFEKISLREGFIMPDWLEVNLKEKSVKFSRFPVEEDLQ
                     EKIDIQSIIEFYSR"
BASE COUNT         2193 a         1482 c         1273 g         3005 t
ORIGIN      
        1 tctttgtgct attataggga taatgtccag aggtagaaag aaaaagttca aattaaaaat
       61 caacgttaaa cccgacactg tcaggtctat tgtatcctta tttttcctgg ctcttttttt
      121 ggttacttta atatcttttt ttgcccctga ttacgaaata aactcaagga ttcaacattt
      181 cttaaaatta tattttggca agtcatctat tttggtacca ttcattttcg gtagttttgg
      241 aatcttattt tttgaccgta ttggtgaaag attcaaggaa gccagaatat tcataggtct
      301 ctttttaatg ttattttcct tgtcggcagt tttacacgtt tttatttcgg aagaaaattc
      361 ttatgatgtt gctttggcgg gaaaaggtgg cggtttaata ggttacagaa tatcaagtac
      421 gctttcttca ggaattagta ttataggcgc ggtagcatta atttttattg gaattttagg
      481 ttcaatattt ttaattttta atctttcttt aaataacata gctgatttct tacaaaaaat
      541 tggattagca gatttgttta aaaagatttt ttctaaaaaa gaaagagacg aagtcgaatc
      601 agagattgaa gtgaacacat cttctgtact tacagttgat gattataaag aagataaaaa
      661 aaatacagct tttgctgatg ttcattcagg agatgctcga gaacctgcac aagcttcgat
      721 atttgaggtc atacaacccc aggccgagcc tttgggtgat tttaaacata tttccaatgg
      781 ttccgttgac acccttgccc ccgtatcttt aataaaagct tctccggcta taccttcgga
      841 taggatttgg gattaccctc ctgagaatct tttagcagag cctccagtac gtgtggttga
      901 cacatcagag ttagataaaa gaataaagat tataaaagat acatttaaat cattttcaat
      961 tgatgttgat attgatatgg aaaatgtaaa agtaggttca tcggttactc agtacgcttt
     1021 aaggcctaag tcagttacaa atatcgcaaa aattgctacg cttcagggaa atattgcttt
     1081 agctctggct tcccctacag gaaccgtaag aattgaagct cctattcctg gaaagtcatt
     1141 aatcggtata gaggttccga atacaaaaag aacaatggtt tattttaaat ctttaattgc
     1201 ttccccgaca atgaaaaacg ctccttccaa gctttcagtg gcgttgggag aagacgttgg
     1261 cggcagggtt gtaacttatg atatcggtaa aatgcctcac cttttaattg ccggaacaac
     1321 cggatcagga aaatctatct ttattcataa cctgcttttt tcaatgcttt ttagatctac
     1381 tccccaggag gttaaattta tattaataga tccaaagaga gtggaactta tactttatga
     1441 cggaattccc catcttttaa cccctgtggt tacggatatg gaaaaagctc cttcagtctt
     1501 tagatgggct gttgaggaaa tggaaagaag atataaatta tttgaaaaag ccagggtgaa
     1561 aaatattgat acctataatg aaaaatcagg catccaggtt atgccttaca ttgtgattgt
     1621 ggtagatgaa cttggagaaa taatgatcca ggaccccgca ggcgttgaaa agactattat
     1681 taggttggcg cagatgggtc gagccacagg acttcacttg gtcctcgctg ttcaaagacc
     1741 ttccatagag gtaattaccg gtctaattaa agctaatatt ccctgtagag tggcttttca
     1801 ggtcttaaat caaatagatt caagggttat catcgatcag cccggtgccg agaagttgct
     1861 ggggaaggga gatatgttgt ttgtaccccc ggatactatg aaaccggtca ggcttcaggg
     1921 tgcttttata tcagaaaaag aaacttctga cttggttaac tttttgaaaa atcagggtgt
     1981 tgcccctgat tataaagaag aagttttaag tatgcccgcg gttacatcaa gaggcggggt
     2041 agtgagatcg agctggggtg atgatgttga cgatgagtta tttgataaag ccgtaaccat
     2101 agttgtttct gcaaaaaaag catcttcgtc tttattgcaa aggaaattga gtataggttt
     2161 tgcaagggct gcaaaactaa tagatatgat ggaagaaaga ggaattgttg gacaggaaat
     2221 gggtggaagc aggggtagag aagttctcat tgacgacctt cctgaagatg gtttagaaat
     2281 tgaaaaagat tcgggttttg atgctgaaga ttttgctggt gagaattttc caagaaacca
     2341 gacagatttt gacgaataac atttttaaaa cggtactaaa tataaaaaaa atcccgtgat
     2401 ttgtgagtat aaaaaaatag agattatagt tttagttcag cttcaataag ttcttccaaa
     2461 tttccgtcta aaactccttc cggattagtt atttcaagat ttgttcttaa atcttttacc
     2521 aacttgtagg gatgtaaaac atagtttcta atttgattac cccaccccgg cattttgtat
     2581 tctcccttta atttcttttn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnt
     2701 ttctaatttg attaccccac cccggcattt tgtattctcc ctttaatttc ttttcttcac
     2761 cttgaatttt ctcctgttcc atctgataaa gcttagattt taatattctt aaggctttct
     2821 ctctgttttt accttgatat ctttcctcct ggcattcaat cacaattccc gagggaatgt
     2881 gccttaatct aaccgcagta gacactttat ttacattttg tccgccggag ccacccgccc
     2941 taaaaaaatc ccactctaaa tcttgatttt ttatttctat ttcctcgtca tcatcaataa
     3001 taggtaacac ttcaacgaga gcaaaagaag tttgtctaag attatcggca ttaaaagggg
     3061 actgtcttac gagtctgtgt acaccagcct ctctttttaa gaatccgtaa gcatatttac
     3121 cctctatctc tatggaaacg gttttaatgc cagcctcttc tcccggtact tcatttattg
     3181 tatatactct ccaatttctt ttttctgcgt atcttaggta cattcttaac aacatagatg
     3241 tccaatccat tgcctcggtg ccaccctgtc ctgcgtggat agacaggatt gcccccttat
     3301 cgtcgtgctt tccggataaa taagttttaa actcaaattc ttttaattct ttttcaatca
     3361 gtacctcgtc tccgttttca agataaaggt agagcatatc aaaatcttca acttcttttt
     3421 ccaaatcaga tatttctttg gataatttat ttccttcctc ccaatttttc caaacatcct
     3481 ctgatttaga cttttccttc aaatcgagca atctctgctt cttttcatct atttttaatg
     3541 tttttttaag ttcttctatt cttctttgaa actcttccat ggaaggatta tatatcaaaa
     3601 acttggtttc cgagcattgt tttatcaaca gggctcaact agcttgaaat aaaatttttt
     3661 aataactttc ctgaaaacaa tccgccgaat aaactcaata gaatgaacaa gaatttacta
     3721 tttttcataa aattacacat atctcctaaa aaaggtatag agaaaaagta tttacctata
     3781 atttcggagt ctgatatagg gctggggtca gggttttcgt ttgcatctcc tttgaaatag
     3841 aaataatatt tatcttgcag cttagaaatt tttgtaattc tgtgagttat aacattttta
     3901 ttttgaagag ttctgtaaga tgcaatatca cctattttga aaacattgtc ctttcgtatt
     3961 aaaaccaaac ttcctttttt gattgcgggg tccattgagg aggtaactac ttttagtatt
     4021 tttatttggt aataatcaaa aattaaataa gtacaaataa acaaaattat tattaaaaca
     4081 gtttttagtt tcgataattt ggatttaagc atattaatca gggggctccg gctgcccgaa
     4141 gggcagccgg agccctttcc tcggttaccc acttgttaaa attaactttg tattgagtta
     4201 aactcaaaat caaatactgc tgaagctcct tgttttagtt cacttattgt tggagctgaa
     4261 aatctaagaa ttaattccat aacctgcccg tagtaaaggt ctcccaatgt aaacccttcc
     4321 gttttccact ttataatact tttctttccc acaacagggc cctcctcacc tgcatctact
     4381 attccatttt gattagtgtc ataccatttt attatttcaa cctgaatgtc tgatcttaat
     4441 tcttgaggat catttgctgt ttcataaaag gcatttgtag atatactaat ttttgaagaa
     4501 gccatactta taagtttcaa caaataatct tgagtccatc ccggaccaat gtttgtaaaa
     4561 gtcggaccag gaagttcatc tttgtaattt gaaggatctg ttcccataga taatgattct
     4621 agaaacatta agtttgcact tcctacacta aaagatgaac ctaaaacctt agctttatca
     4681 gagaaagctg cgtatgttat gccggcaatt aaagaaataa cgattaaaga tgacccgatt
     4741 aaatatagta attttttctt tttcatattt tcacctcctt tctaaataat taaatactaa
     4801 taaggttttg tgctttcatg ctcataaaat cattgctggt tactataagg tgatctgtta
     4861 aaggtattcc catgtctatt cctgccctaa atattctttt tgttaccttt atatcctcgt
     4921 cgctcgggtc gggttcgtta gaagggtgat tatggacaag tattattgaa taagcatttt
     4981 tgtttagggc ttttttaaaa atttcacgag ggtgtattag cgtttcatta atagttcctt
     5041 tagatatgac ctcttgtgat atcaatctgt ttctgctatc cagacagatt aaaaataagt
     5101 attcctcttc tttgttgata acctccttcc taatcagatc atagacatcc tttggacttt
     5161 ttatttttat ggcgtcttct ttgaaaggtg ttaaataacg ttttgatatt tcctccacag
     5221 cttttattga ggttgccttt gcaatatcaa tatttttaaa gtttttgagt tgctcaatat
     5281 ccgccgatag aaccttcttt aaattgccaa attttaaaat aagttccctg gaaagaccgg
     5341 tagcggacgt atcttttcct cctgatctta ttattatcgc cagcagctca acatctgtaa
     5401 gaacttcaga tcctttttca accatttttt ctcttggttt ttcttctttt tgtaaatctt
     5461 taatcttcat attatctttc ttggatataa tctatatcac nnnnnnnnnn nnnnnnnnnn
     5521 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5581 nnnnnnnnnn nnnnnnnnnn actccgatcg cgtggaaagg tttttcccac tttgttttat
     5641 ctacattaga ttcaaaataa tcgtttatac ttttgccatt aacggtgaac tcaccctttt
     5701 tatcccataa aaataccctg gcaatagctg ttttccttct tcctgttcca acaaaaaacc
     5761 ctttcttacc gggctttgtg tcaacagctt tttcttctgt tttaatctct tcttttgttt
     5821 caacttcttc attttcttct ttttctattt ccatttctat ttctttatct acaacaacct
     5881 tctcttcctt aactttttgt tcttttggtg ttgaggtttt ttttgcaact actttttttt
     5941 cttttttatc ttttttttct acagccattt tatttactta attgagattg atgcggatga
     6001 ttttcaccgg cataaatata taaattattt aatctttctt tctttagttt attttttggc
     6061 aacattcccg taacagcttt tttcaaaact tcggtaggct ttctttctaa aagtgactct
     6121 aatttttcag ttctcaaacc atgaggaaac ccggtgttgt gaatgtattt tttatctttc
     6181 atttttttac ctgtaacctt tattttttcg gcattgataa caactacctt gtcgccccag
     6241 ttttgagatg gtgtatattc cggtttgttt tttccgttta gtatcaagga gattttctca
     6301 gcaactttac ccaatgtctg atctttagca tctattaaat accatgcttc tttcttttcc
     6361 ttaatattta aaaatgttgt tttatttggt tttgtcttca tttttcttta ccgccttttt
     6421 ctttaatttt tctttagttt cttcaagact cgaaaccagc tcaattcttg ctgtcagcga
     6481 attatctcca tctctattac ctgtttttat aatcttagta taacctcctg ctctctctaa
     6541 aaacttgggt gcaatctctt ccattagttt ttttatcact tcctcagatc taagactttt
     6601 tcttaaagcc ttcactgtgt tgaatttaaa aatcttatcg tctgatttgt gagctttaat
     6661 tgctttggtt ataagttttt caattttagg ctttacccac ttggctttcg cgagagttgt
     6721 gtttattttt ccgttctcaa taatttgtga caacaggttc agagctagtg attttctgtg
     6781 atccatgtct ctgtttaatt ttttctttac aactctatgt ctcattttat attcccttta
     6841 ttttctatat ccttccttat ccaaaagatc tttaatttcc tgtaatgact tttctccgat
     6901 atttttaata tcctctaatt cttcgttaga cttttttgct aattcgtgca aagacattat
     6961 cttgcttttc ttaagtgcgt ttatggttct ggtttgaaga ggaagttctt caactgatac
     7021 atcgtctccc atctttacac tcgattttgg tttttctttt acttcttcct cgacatcagc
     7081 tttccattcc tgaaaactaa tataatattc ttttaatatc tttgaggctt cttttatggc
     7141 gtcttttgga gaaatacttc cgtcagtctc tattgtaaaa gtaatcttat ctaaatcagt
     7201 tcttccgcca aatcttgtag gtactatttc gtaattagca cttataatag gggagaacag
     7261 tgcgtctaaa acgattactc ccatttttga ggtctgtctc tcttccatag gtgaatatcc
     7321 aataccttgt tctataacca tttccatgtc tagcttagat tttgaatcgg ctaatgtagc
     7381 gataagttga tctttgttca tgatttcaac atctgatgtg gtttcgatat ctttagcggt
     7441 tatttttccg ggaccttttt tacttatggt tactaccacc ggttcatcgg aatggctctt
     7501 aaatcttaat ttcttaagat ttaaggttat ttcaacaacg tcttctttaa caccatttat
     7561 agtagtgaac tgatgtgtag cgcctgcaat ttttacttgt gttgcagcag agccttttat
     7621 ggatgacatc aagactcttc taagagcatt tgctaaagtg tatccaaatc ctgttggtaa
     7681 cggctcgtaa gaataaactc ccatatttcc ttcttctttg atggtgttta tttttaaatt
     7741 ttcttttgta acttccatat tttcctcctt aagactccgc ttcagcggaa tttaaaaaat
     7801 ttatctcgaa taaaactcta tgattgactg aatatcaatc ttctcctgaa gatcctcctc
     7861 aacagggaat cttgagaatt taacactttt ttctttaaga ttgacttcaa gccaatcggg
     7921 cataataaaa ccttctcgca aactaatttt ttcaaaactt tcgggcttaa tgatgttgcc
     7981 cactttaatt tccattgaag gtatagtgac ttttttacca tttactgtaa ataaacctct
     8041 tctaatattt tgtcttgcct gtgctcttga aactgcaaat ccgcttctgt aaaccaagtt
     8101 gtcaaatctc gattcaagtt tttgcattaa aatatttcct gaaacacctc ttg
//
LOCUS       DAOTFA010000015         7858 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate AMR_MDS_3115
            NODE_6540_length_7858_cov_7.073433, whole genome shotgun sequence.
ACCESSION   DAOTFA010000015 DAOTFA010000000
VERSION     DAOTFA010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868139
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (wastewater metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7858)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7858)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:36:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 536
            CDSs (total)                      :: 521
            Genes (coding)                    :: 520
            CDSs (with protein)               :: 520
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7858
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6540_length_7858_cov_7.073433"
                     /isolate="AMR_MDS_3115"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            732..3731
                     /gene="uvrA"
                     /locus_tag="P5014_01055"
     CDS             732..3731
                     /gene="uvrA"
                     /locus_tag="P5014_01055"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007059250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRY56730.1"
                     /translation="MKDSISHILVRGARQHNLKNVDLDIPKNKMVIFTGVSGSGKSSL
                     AMDTIYAEGQRRYVESLSAYARQFLGVMDKPDVDKIEGLSPAIAIDQKTTSKNPRSTV
                     GTITEVYDFLRLLYARVGHPHCPECGREIERQSSQEIVQNVFEIIKNEPEFKSERGVR
                     FIIFSPVVKDRKGEFSSLFENLKKQAVLRARVDGIVRELSDDFNLIKTNKHSIDALIS
                     RHTINQKKIKNEEDRKEIESEVYQSVETALRISSGNAVVTIVKDESFDFSQNPKKFDD
                     HLFSENFACPVCNISLPELEPRTFSFNTPYGACPACDGIGSQLKVDPDLVINPRLSIS
                     EGGVLPWTRLFEHISWTSKVVKSVADYYDFSIDTPIGELSKEAKNILFFGAEGKKFKV
                     TYNSQEGSKRTFDANYEGIIPNLSRRYRESESDFVKNEIEKYMVKERCPVCLGARLKK
                     ESLSVTLGKKNIDEIVQMPVNKLQDWFKDLSDKLNSREKDISSTILKEINYRLKFLVD
                     VGLGYLTLGRASAELSGGEAQRIRLASQIGSGLSGVLYVLDEPSLGLHQVDQGKLIKT
                     LKQLRDLGNTVLVVEHDAETILESDYVFDFGPGAGEHGGKVVSEGTPDQIKKDKNSLT
                     GRYLSGDLIVGKNPIKGKRTFEDILEEESRSKLNKKVEGKSLILKGAKGRNLKNIDLN
                     IPLGKFICVTGVSGSGKSTLIMDTLYQGLRQEFGLKIEEKPEPFEYIKGVENISNVIA
                     IDQSPIGRTPKSNPATYTKVFDHIRDLYAQTQEAKIRGYNQGRFSFNVKGGRCEACGG
                     EGQIKIEMQFMPDVYVRCDVCKGKRYNSEALEIEYKGKNISDVLNMTVEEAVSFFENL
                     PQIRRKLETLYDVGLGYIKLGQPATTLSGGEAQRVKLALELSKRSTGSTLYILDEPTT
                     GLHFADLENLISICKRLTARGNTVLMIEHNLDMIKNCDWVIDLGPEGGDSGGEILFEG
                     NLEDIKKCKKSYTADSLRLIDKYYKSN"
     gene            complement(3733..5466)
                     /locus_tag="P5014_01060"
     CDS             complement(3733..5466)
                     /locus_tag="P5014_01060"
                     /inference="COORDINATES: protein motif:HMM:NF013961.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HRY56731.1"
                     /translation="MNTCFKNIVRTLVSVFILTNIFITSGVIFKVEASQFMIDYKSEY
                     IIDESGEANITHNATITNLQNDVIPMNYSLSAMDSNIYEVSAETNGKETEPKLNNNKN
                     NTDITVTIKDYSIGEGRQNTITIKYKSKSVAVKSGSIWNIYIPKIQIPDETTAYDFKL
                     SVPESFGPKVFLTPEPTIEKKEDGKNIFYFTKNTFESTGISAAFGGFQPVNFKIKYQI
                     QNDLILPAIKKIAIPSDIEHYQNVSYDKISPRPFKIEYDEDNNFVAYYILKPKQKTEI
                     EVQGTSRLLSKQINVNSGGIISEIPKVLIKNYTRQQKYWETKSPYILKIARELKKDDQ
                     TVVYNAKMVYEFIKNNLKYDFDAQSKGMVERKGAEAAASQKNSWTCMEFTDLFIAVTR
                     AMGIPAREINGYAFTSEESNKPISISLESGDTLHSWAEFYDPYNGWVQVDPTWGTTSG
                     LDYFSKLDTNHFAFVRKGISSEHPFSAGTYRYSDNEKLIETALSQTSSDENFKPSVKA
                     NKVLNLNPLVILSGKIRIKIENTGKVTAYDINDLNILPGEKRYTNIKKENDRVVFKDF
                     MGNEFETEIEK"
     gene            5490..6845
                     /locus_tag="P5014_01065"
     CDS             5490..6845
                     /locus_tag="P5014_01065"
                     /inference="COORDINATES: protein motif:HMM:NF020048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HRY56732.1"
                     /translation="MKTSNVVQKVENLPQTPGSYIFKDSTGKVIYVGKAKNLKARVKS
                     YFSVNIPSDFKTAELVRRINDLEFIKVDSEFEAIILEAELIKKYKPKYNIVLKDDRSK
                     IYIVIRNDLITLSEKKYKLPKVITARKPDLKETDIVFGPYPNNVVVRNILTTSRKIFP
                     FRDCNYSKFAKYKKIGSPCLYGHMGICSSPCLANEVSDLSDYKKSISGLKKILSGNVT
                     GLIRDINKKMQTYSKNENFEEAAVCRDLLDKFYFVQKTFREAEEYINNPYLVDDISKK
                     SLEEIKDFLPVLKNLPRRVECYDISNISGKEAVGSMVVSINGTIDKSEYKRFRIKFKK
                     TPDDFGMLYEVLYRRLKRELPGSDMKSWGVPDLIVVDGGKPQVSSASDVLRDLEILVP
                     VVGIAKKYETLVYKEGKDFIEKTFPKDSLGMKLIIKLRDESHRFAQSYHHLLRKKSLL
                     E"
     gene            6895..7299
                     /locus_tag="P5014_01070"
     CDS             6895..7299
                     /locus_tag="P5014_01070"
                     /inference="COORDINATES: protein motif:HMM:NF015953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HRY56733.1"
                     /translation="MRSILRALVYTFISLYFTNLIVGSFSYTNDKTAYLVVFAISLLY
                     LLLRPVLSVVSLPTKGSVFLLITVITTSIIFYALQSVLPDFSFVPVTLRSLNIFGRVL
                     PSKDLDSLWAMIFSALMTSAIYLFLESLCKRR"
     gene            7302..7520
                     /gene="secG"
                     /locus_tag="P5014_01075"
     CDS             7302..7520
                     /gene="secG"
                     /locus_tag="P5014_01075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HRY56734.1"
                     /translation="MLAFTKTLQLIISGLLIVLVLIQSKSSGLSSTLKSSFNMYRSLR
                     GFEKIIFVLTVISGVLLVVNSLAIVLLS"
     gene            7522..>7858
                     /locus_tag="P5014_01080"
     CDS             7522..>7858
                     /locus_tag="P5014_01080"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="adds enolpyruvyl to UDP-N-acetylglucosamine as a
                     component of cell wall formation; gram-positive bacteria
                     have 2 copies of MurA which are active; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HRY56735.1"
                     /translation="MPGIFIEGGTPLMGTVSVSGAKNSASKLIYASMFSNEDVILNNV
                     PKIKTIMDDIEVIQSVGGRAEWVGTNSLSLNGSQINTYEVPVEIGSKFRTSTLLAGPL
                     LYRFGKAFIP"
BASE COUNT         2618 a         1140 c         1481 g         2619 t
ORIGIN      
        1 tgaaagacct gctaatgtgt ttgtaaaaaa agatgacagg tgtggaattt cggaaatatt
       61 tattaaaggg actgtgattc ctagcagtct ctgtccggag aaagagaaag agaaagagaa
      121 agagaaggaa gatgataagg atggaggaaa gaatgaaaag aatagataaa aaagagacga
      181 caaaaaccta aaatttaaac tatattcaaa ccctttacat atttgataaa aaactcaaga
      241 taacgtagta tcgtagaaaa ttcagaattt ttttaatatc ttacagatac ttaaaaaata
      301 agtaacaaaa tataaaggag atgaaaaatg ttgcaagggc tgatgaaaag ttggtggttt
      361 tgggtatcga tttttattat tttttggcca ttaaatttta ttttttggca aaatatcaaa
      421 aaagggtcaa gaagcatcaa caccttttta gaggagtggg atcagttgta taaatatccc
      481 tttagtcgtg actacgttta tgacattatg tccgttttga gctttccggc tctcttgttt
      541 tatgtaatat cattcgtgtt tatttttgtt attaccagac ttgctgaacc tttcctttgg
      601 acatgagatc ggatccgtgt agactttaaa gccaggttat tcaatatttg atgacttggc
      661 tttttcatta aatcaaaccc aaataaaacc cgtagacaca tatggcgttt atcttgtaca
      721 atgtttaagt tatgaaagat tcaatttcac acatcttagt taggggtgcc cgccaacaca
      781 atttaaaaaa tgttgatttg gatattccta aaaataaaat ggttattttt acaggtgtct
      841 ccggatcagg aaaatcttct ttagcaatgg atactattta tgcagaaggt cagagaagat
      901 atgtggagag tctgtccgct tatgccagac agtttttagg tgtgatggat aaacctgacg
      961 tagacaaaat agaaggttta tctccggcta ttgctataga tcaaaaaaca acaagtaaaa
     1021 atcccagatc tacagttgga accataacgg aagtttatga ttttttaaga cttctttatg
     1081 caagagtggg gcatccccac tgtcccgaat gtgggagaga aatagagagg caatcttctc
     1141 aggaaattgt tcaaaatgtt tttgaaatta ttaaaaatga accggaattc aaaagtgaga
     1201 gaggagttcg tttcataata ttttcacctg tcgtaaaaga tagaaaaggt gagttttctt
     1261 ctttgtttga aaatctaaaa aaacaggcag ttttaagagc aagggttgat gggatagtta
     1321 gagaactttc agatgatttt aatttaataa aaactaataa gcatagtata gacgcactta
     1381 tatccagaca cactattaat cagaaaaaaa ttaaaaatga agaagataga aaagaaattg
     1441 agtccgaggt atatcaatcg gtcgaaactg cattaaggat tagctcagga aatgctgttg
     1501 taacaatagt aaaagatgaa tcttttgatt tttctcagaa tccaaagaaa tttgatgacc
     1561 atctgttttc tgaaaatttt gcctgtcctg tttgtaacat atcattacca gagctcgagc
     1621 ccagaacatt ttcatttaac actccttatg gagcctgccc tgcctgcgac ggaattggaa
     1681 gtcaattaaa agttgatcct gatttagtta taaaccctag actttcaata tcagaaggag
     1741 gagttttacc ttggacaagg ctttttgaac atatttcatg gacatcaaaa gttgttaaat
     1801 ctgttgcgga ttactatgat ttttcaattg atacacctat tggagagtta agtaaggaag
     1861 caaaaaatat attatttttt ggtgctgaag gaaaaaaatt taaagtaact tacaacagtc
     1921 aggaaggttc aaaaagaaca tttgacgcaa actacgaggg aatcattcct aatttatcaa
     1981 gaagatacag agaatctgag tcagattttg taaaaaacga gatagaaaaa tatatggtta
     2041 aagagagatg ccctgtttgt ttaggagcaa ggttaaaaaa agaatcttta tcagttactt
     2101 taggaaagaa aaatattgat gaaatagtac aaatgcctgt gaacaaactt caagattggt
     2161 ttaaagactt atcagataaa ttaaattccc gagaaaagga tatttcatca acgatattaa
     2221 aagaaattaa ctatagatta aaatttttag ttgacgtagg cctcggttac ttaactttag
     2281 gaagagcgtc ggcagaatta tcgggaggcg aagcacaaag aataagatta gcttcacaga
     2341 taggttcggg cctaagtggt gttctctatg ttttagacga accttcctta ggattacatc
     2401 aagttgacca gggaaagtta attaaaaccc ttaaacagtt aagagatctt ggaaatacag
     2461 tacttgttgt tgagcacgat gcagagacaa ttttggaaag tgattatgtt tttgactttg
     2521 gtcccggagc gggggaacac ggtggtaagg tagtttcaga gggaacaccg gatcaaatta
     2581 aaaaagataa aaattcactt accggaaggt acttatcggg agatttaatt gttggtaaga
     2641 atcctattaa aggtaaaaga acatttgagg atattttgga agaagaatca agaagtaaat
     2701 taaataaaaa agttgagggt aagagcttaa tattaaaagg agcgaaaggg agaaatctta
     2761 aaaatattga tttaaatatt ccactaggta agtttatatg cgtaaccgga gtttccggtt
     2821 ctggtaagtc tacattgata atggatactt tgtatcaagg attacgacaa gaatttggtt
     2881 taaaaattga agagaaaccg gaaccatttg agtatataaa aggtgttgaa aatatttcta
     2941 atgttattgc tatagaccag agtccaattg ggagaacccc aaaatcaaat ccggccacgt
     3001 atacaaaagt ttttgatcat attagagatt tgtatgcaca gacacaggaa gcaaaaatca
     3061 gagggtataa ccagggaaga tttagtttta atgttaaagg tgggaggtgc gaagcttgtg
     3121 gaggtgaggg acagataaaa attgaaatgc aatttatgcc tgacgtttac gttagatgcg
     3181 acgtttgtaa gggtaagaga tacaattcgg aagcattaga aatagagtat aagggtaaaa
     3241 atatatcaga cgttttgaat atgacagttg aggaggcagt atcatttttt gagaatctac
     3301 cccaaattag acgtaagttg gaaactttat atgatgtagg tcttggatat attaaacttg
     3361 gacagcccgc gactacattg tcaggcggtg aagcacagag agtaaaactt gcacttgagt
     3421 tgtcaaaacg atccaccggg agcactctat atattttaga tgaaccaacc accgggcttc
     3481 attttgctga ccttgagaat ctcatttcca tatgtaaacg tttaaccgcc cgaggtaaca
     3541 ccgttcttat gattgaacat aatttagata tgataaaaaa ctgtgattgg gttatagact
     3601 taggtccgga aggaggagat agtgggggag agatactgtt cgagggaaac ctcgaggaca
     3661 ttaagaaatg taaaaaatct tataccgctg actcactcag attgattgat aagtattaca
     3721 aatcgaatta gtttactttt caatttcagt ttcaaattcg tttcccatga aatctttgaa
     3781 gactacccta tcgttttctt tttttatgtt ggtatatctc ttttctccgg gaagaatatt
     3841 taaatcgttt atgtcatatg cagttacttt tccagtattt tctatcttta ttcttatttt
     3901 cccagataat attacgagtg ggttaagatt aagaacctta tttgctttta ctgatggctt
     3961 aaaattttca tcagagcttg tctgagataa ggccgtttct attaactttt cattgtcaga
     4021 atatctatat gtacctgctg agaatggatg ttcagaactt attccttttc taacaaaagc
     4081 aaaatgatta gtatctaatt ttgaaaaata atctagtccc gatgtagtac cccatgtagg
     4141 atcaacttga acccagccat tgtatggatc gtagaattca gcccatgagt gcaatgtatc
     4201 cccactttcc aaactgattg agatcggttt attagattct tcggatgtga aagcataccc
     4261 attaatttct ctggcgggta ttcccattgc tcttgtcaca gctataaaaa gatcagtaaa
     4321 ctccatacag gtccatgaat ttttttggga tgccgctgct tcggcacctt ttctttcaac
     4381 catacctttg ctttgtgcat caaaatcata ctttaaatta ttttttataa attcatatac
     4441 catttttgcg ttataaacta cagtttgatc atctttttta agttcccttg caatttttaa
     4501 aatataaggt gatttggttt cccaatattt ttgctgtctt gtgtaatttt ttataagtac
     4561 tttaggaatt tcagatatta ttcctcccga gtttacatta atctgtttac ttaaaagtct
     4621 tgatgttcct tgtacctcaa tttctgtttt ttgctttggt tttagtatgt aataagcaac
     4681 aaaattatta tcctcatcgt attctatttt aaaaggtctt ggacttattt tatcgtagct
     4741 gacgttttgg tagtgctcga tatcagaggg tatagctatt ttcttaatcg ccggaagtat
     4801 taagtcattt tgaatttggt attttatttt aaagttaaca ggctgaaaac ctccgaaggc
     4861 ggcactgatg ccggttgatt cgaaggtatt tttagtaaaa taaaatatgt tcttaccatc
     4921 ctcttttttt tcgattgtcg gttctggagt taaaaatact ttcggtccaa aagattccgg
     4981 aactgacaat ttaaaatcgt aagctgtcgt ctcatcaggt atttgaattt ttggaatgta
     5041 gatattccaa atactgcctg attttactgc tacacttttt gatttatatt ttatggtaat
     5101 tgtattctgt cttccttctc cgattgagta atctttaatg gttacagtta tgtcagtgtt
     5161 atttttatta ttatttaatt taggctctgt ttctttccca ttggtttcag cggatacttc
     5221 gtatatattt gaatccattg ctgataatga gtaattcatt ggtatgacat cgttttgtaa
     5281 gtttgttatt gtcgcgttat gggtaatatt tgcttcccct gactcatcaa taatatactc
     5341 tgatttatag tcaatcataa actgacttgc ttcgacttta aaaattacac cggatgtaat
     5401 aaaaatgttg gtgagtataa aaaccgaaac tagtgttcta actatgtttt taaaacaagt
     5461 attcatcctc caaatgctac aataactatg tgaaaacctc aaatgttgtt caaaaagttg
     5521 aaaaccttcc gcaaactccg ggttcgtata tttttaaaga tagtactgga aaggttatct
     5581 acgttggtaa agccaaaaat ttaaaagccc gagtaaaatc ttatttttct gtcaacatcc
     5641 cctctgattt taaaacagct gaattagtac gtaggattaa cgacctcgaa ttcataaaag
     5701 tagattctga atttgaagcc ataatattag aagcggaatt aattaaaaaa tataaaccta
     5761 aatataatat tgttttaaaa gatgatagat ctaaaattta tattgtaatt agaaacgatc
     5821 ttattacact ttcggaaaaa aaatataaat taccaaaggt cattactgct cgtaaacctg
     5881 atttgaaaga aactgacata gtgttcggac catatcctaa caatgtagtt gtaagaaata
     5941 ttctcacaac atcaagaaag atatttccat ttagagactg taattattcg aaatttgcta
     6001 agtataaaaa aatcggatcc ccatgtttgt acggtcatat gggaatttgc tcttctcctt
     6061 gtttagcaaa tgaagtctct gatttatcag attataaaaa aagtatttca ggtttgaaga
     6121 aaatactgtc tggaaatgtt acaggtttaa taagagacat aaataaaaaa atgcagactt
     6181 actcaaaaaa tgagaatttt gaagaggctg cagtgtgcag agacttatta gataaatttt
     6241 attttgtaca aaaaactttc agagaggccg aagagtatat taataaccct tatcttgttg
     6301 atgatattag caaaaaatct ttggaagaga taaaagattt tttaccggtt ttaaaaaatt
     6361 tacctcgaag agttgagtgt tatgatattt caaatatatc aggcaaagag gctgtaggtt
     6421 caatggtagt ttctattaac gggacaatag ataaatcgga atataaaaga tttaggataa
     6481 aatttaaaaa aacaccggat gattttggaa tgttgtatga ggtattatat agaagattaa
     6541 aaagggaatt accgggaagt gatatgaaaa gttggggagt tccggacctt attgtcgttg
     6601 acggaggaaa accgcaggtc tcctctgctt ctgatgtttt aagagattta gagatattag
     6661 ttccggttgt aggtattgca aaaaaatacg agactttagt ttataaagaa ggtaaggatt
     6721 ttatagaaaa aacatttcct aaagacagtt taggaatgaa attaattata aaattaagag
     6781 acgaatccca cagatttgct caaagttacc accacctact aagaaaaaaa tcactactag
     6841 agtgattttt ttcttttgct tttgtactct caatagattt ataatctaat caatatgaga
     6901 agtattttaa gagcgttggt ttatacattt atttctttat attttacaaa tttaattgta
     6961 ggatcgtttt cttatactaa cgataagacc gcatatttag ttgtgtttgc gatctctctt
     7021 ctatatttac tattaagacc ggtattgtca gttgtttcat taccaaccaa gggaagtgtt
     7081 tttttattga ttacagtaat aacaacttct attatttttt acgctttaca aagtgtgctt
     7141 cctgattttt catttgtacc cgttacactg cgcagtttga atattttcgg gcgtgtgcta
     7201 ccatctaaag acttggatag cctttgggct atgatatttt ctgcccttat gacaagtgcg
     7261 atttatttat ttttagaaag tctttgtaaa agaaggtaat tatgcttgca ttcacaaaaa
     7321 cattacagtt aataatttcc ggccttttaa tagttctggt tttgatacag tcgaaatctt
     7381 caggtttgtc atctacattg aaaagttcct ttaacatgta tagatcacta agaggttttg
     7441 aaaagataat ttttgtcctt acggttatat caggggtttt actagtagta aattctttgg
     7501 ccatagtttt gttgagctaa tatgccggga atttttattg aggggggtac ccctttaatg
     7561 ggtaccgttt cagtttcagg agctaaaaac tcagcttcta agcttattta cgcctccatg
     7621 ttttcaaacg aggacgtaat tttaaataat gttccaaaaa taaaaacaat aatggatgat
     7681 attgaggtta ttcaaagtgt cgggggaagg gcggaatggg ttggtacaaa ttcgttgtcc
     7741 ctcaatggat cccaaataaa tacctatgaa gtacctgttg aaattggttc aaaattccga
     7801 acgtccactt tattagctgg gcctctttta tataggttcg ggaaggcttt tattccaa
//
LOCUS       DAOTFA010000016         7685 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate AMR_MDS_3115
            NODE_6735_length_7685_cov_5.787680, whole genome shotgun sequence.
ACCESSION   DAOTFA010000016 DAOTFA010000000
VERSION     DAOTFA010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868139
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (wastewater metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7685)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7685)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:36:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 536
            CDSs (total)                      :: 521
            Genes (coding)                    :: 520
            CDSs (with protein)               :: 520
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7685
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6735_length_7685_cov_5.787680"
                     /isolate="AMR_MDS_3115"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1783)
                     /locus_tag="P5014_01085"
     CDS             complement(<1..1783)
                     /locus_tag="P5014_01085"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRY56736.1"
                     /translation="MNYLYLVVIFLIFVSSYILVLKNFKFSLYVLIVLSVLMHKELFS
                     FYRWDLMPARIFMLALLFAGITKTYLYVVKSGGIKSLLPYIKDPYILTLLIIFFTRAL
                     SLVFSKNIQASIFLLGFFATIVVLGIYLYLTFKDNPQELLKYLKFFTYVVFTLTLFGY
                     YQLFHFYQTGRVIGAMWPVPGKVPRVGSLFWDVNHYGALLSALIPVILISFVNTRNKK
                     EKLLDVFMVLSYSASLLLTNSRTAWMVAGFSAVFFFTILLIKKFGAKGILYIFLALVL
                     ITSPFVVEYSKKKSNFRRYVRDAFHYRIDSFDSHFMLLTGAYQIFEKYPYLGGGYGSF
                     FEHFSKTKIAPKFFTRDTAALSGRVPAHTIWGESLSETGIIGTVPFVSFMLFAFLSPL
                     YVFFKNKDKKVAFTGGLMSSVILGWYLAGIFYSYNSEFFWLLIFSYFIWGIGIIGKDW
                     IKKIIQYIFVNKNIMFSTILILSALLIFTGLGKNHLIPWDEAIYAKVAKNMLINKEFV
                     VPHWDNLTKGWFEKPPLYMWMMSGFMSFLGFNSWAARLPSAIFGFFTVILVYLFSKKF
                     FNKTSAFISSLALVTTVHFLYYSRASML"
     gene            complement(1780..2970)
                     /locus_tag="P5014_01090"
     CDS             complement(1780..2970)
                     /locus_tag="P5014_01090"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRY56737.1"
                     /translation="MKVLMLGRIGLLDARGGDRVQVENTASELKKLGIDVDIKTDMDF
                     EPKDYDLVHIFQLDWTPETYFYAKKVKKAGVPIVLSPIHHDVKEVKKFDDEFVFDFRR
                     ISKLFFRDQFRRDTFKNVYRSLFDRRKFKPTLYSVFHGFKKMQREVLEISDAVLVQTV
                     REAEDLKRTFDVTFKWEKVLNGVGEAFIKPKPFKNPFEFEDYIMCVGRIEPRKNQLNV
                     IKGVKKFREETGLDAKLVLIGKASPMKHFEYNFLINKLLRKHKWITRLDNSINYSDLP
                     SYYHFAKVCVSASWFETTGLTSLEALYCGANTVAAGPRAKEYLGSFASFCVPDNVESI
                     TEAIKKEYFEPRPVLDEKIRKEYTWQNAAEKTLSVYEDVLSSMKPEVGETINKKPKSS
                     KKKK"
     gene            complement(2967..4118)
                     /locus_tag="P5014_01095"
     CDS             complement(2967..4118)
                     /locus_tag="P5014_01095"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRY56738.1"
                     /translation="MKILYVLNSSKFGGMEVHVLDLVKGMVKKGHEVFVWCPQGDMSD
                     LYQKNGAVVSNEFISKDFDFKYIANLKKYVEENNIDIVHSHELKAVVNSLLACRNTKV
                     KAVVTHTHTPISEWQINSIKKFLNVKLYSYVVNKLSSAEIALTESKKKVKIKEGINEK
                     ILEVIPNGIDTSIFNIPALHRFEFEKEIKRRHGIPEDAFVFGNLGRITQEKGHEVLVR
                     AFSKFLNSQLFRKEKMYLLIVGGGALEDKIKNLVKELELDKNVVITGRFDDEDKIKYL
                     STFDVFVFPTLAEGFGIVLIEAMSMGLPVICSNLEVLKEVGEDYVKFFNVGDYEDLSD
                     KMVDEYNKTGSENKNLVEGAREFVENNYSLESFVDKYDYLYLKLMGDKK"
     gene            complement(4115..5323)
                     /locus_tag="P5014_01100"
     CDS             complement(4115..5323)
                     /locus_tag="P5014_01100"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRY56739.1"
                     /translation="MTIEEPKYNIICLSNQLWDFPLWTNKRHVMSRLPKLGHSVLFID
                     PPINTGRVFAKQILQRNWSLKRLFTKTKKTEEGVVVYSPLNTHPLKGISSAKHSQKIN
                     EISQKVFKDSGNEKTILWIYHVEIEGLENYLKNIKHDFLIYDCVDNYEGFPKYNTPEK
                     KRWIVDQERLLTSRANIVFTTTPGLAEKLKKINPKTYFTPNVGDFERFFDVKNKGFEV
                     PEDLKNIPHPIVGFTGAVDEYKFDRELFKKIADDYPSFSFVVIGPLALKDKESSKKSL
                     GFEKLDNVYFLGRKDFTEIPKYISFFDVMTIPYQLNDYTVGGCFPVKFFEPLAAGIPL
                     VVTDLPSYYPFEDVCYISKNPEEFSLNIKRALEEDIAEKSKKRMEVAKKNTWEGKIAA
                     MLSYINENIK"
     gene            complement(5444..6835)
                     /locus_tag="P5014_01105"
     CDS             complement(5444..6835)
                     /locus_tag="P5014_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56740.1"
                     /translation="MNSKKFFQIVFISFFIYFGSWLLVYKTNINKLAIQSEDTVPAMF
                     LPVAIIKEKTLYLGSYYNMLISKFPHPDDKNQIRGLTPFYLKKIDGHYVSAFTLISGL
                     LAVPVYFLPLKFGMVVTWENLIILSKISSALIMALSGGFFYLLLKKQFSLDEKKASVL
                     TYVFLFATVNLAMLSQSLWQHGTLQLFSILGLYFVLDHLKSPNFKPFSAFLSGLFYGL
                     AFLSRPTAALGLLFIGIYALIKLNNLKSILKTGVFVALGFLLNVAFFLWYNNKYYIGI
                     ENQGYASQLFGSWISPFPVSFFGVWLSPSKGILTYSPVFLFSFVGFYIAVKKGLKENL
                     QYLFYFLIVVIHTLVISFWKHWYGGYSFGYRMSSDVIPYLVLLMVPYLKSSWYDKKHF
                     LFSLLFGFSILVQAFGIVFFDGIWHAAYDTGFKNTSWLWSLKDSEFMFNVRRLLVKAK
                     MLERACPKCLPLK"
     gene            complement(6923..>7685)
                     /locus_tag="P5014_01110"
     CDS             complement(6923..>7685)
                     /locus_tag="P5014_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56741.1"
                     /translation="SEPVFVDNYLVYTPNNSRVENKNAKDFKNEGVFYIQNGDLEDIV
                     LAINSSENLSNELRSEEPSNFFQIKNLKKEFGSSEPEIQITFSCKRPDIGTDKIKVDF
                     YYLEEGVVGHDDKTLLYFPGCSKIGEPETYKVKLEIPFTDSEFLFIRLVDVNSSDIKD
                     IEIINGDQVYLPEYFSKGRGYEIIDSGGLTTSAIVPVTNYIFGDSYDLGFDLNFDEEG
                     KFDISVPINGLIKEGKLKDRFLIWSTKKYLPVRLP"
BASE COUNT         2651 a         1490 c         1143 g         2401 t
ORIGIN      
        1 caagcattga agctcttgaa taataaagaa agtgaacggt tgttaccaac gccaaagaag
       61 aaataaaagc tgatgtttta ttaaaaaact ttttggaaaa taaatagact agtataacag
      121 taaaaaatcc aaaaattgca ctcggcaagc gggccgccca agaattaaat cctaaaaagc
      181 tcataaatcc tgacatcatc cacatataca aaggaggttt ttcaaaccat ccctttgtta
      241 aattgtccca atgaggaaca acaaactcct tgtttataag catattttta gctacttttg
      301 cgtaaatagc ctcgtcccac ggaataagat ggttttttcc gagacctgta aaaataagga
      361 gagccgaaag aattaaaata gttgaaaaca ttatgttttt atttacaaaa atatattgga
      421 tgattttttt aatccaatct ttaccgatta ttccaatccc ccaaataaaa tatgaaaaaa
      481 tcagtaacca aaaaaattca gaattgtaag agtaaaaaat acccgccaaa taccaaccca
      541 aaatcacgga agacattaaa cctccggtaa aagcaacctt cttatcttta tttttaaaga
      601 aaacatacaa aggtgataaa aaggcaaaca acatgaacga gacgaaaggt acagtaccaa
      661 tgatacctgt ttctgacaga gactcacccc aaatggtatg ggccggaact ctcccgctca
      721 atgccgcagt atctctagtg aaaaatttag gagcaatctt tgtcttgctg aaatgttcaa
      781 aaaaagaacc gtagccacca cctaagtacg ggtatttttc aaaaatctga tacgctcctg
      841 tcaaaagcat aaagtgagaa tcaaaagaat ctattctgta atgaaaggca tcccttacat
      901 atcttctgaa attacttttc ttcttactat actcaaccac aaaaggcgac gttattaaaa
      961 ccagagctaa gaaaatatac aaaataccct tagctccaaa ttttttaatt aataaaattg
     1021 taaaaaagaa tacagctgaa aaacctgcaa ccatccatgc agttctggaa ttagtcaaaa
     1081 gaagagatgc ggaataagat aacaccataa acacatccaa taatttctct ttcttatttc
     1141 tagtatttac aaatgaaatt aaaatgacgg ggattaacgc agacagtaaa gccccataat
     1201 gatttacatc ccaaaacaaa gaacctactc tgggtacttt accgggaaca ggccacatgg
     1261 ctccgattac tcttcctgtt tgataaaaat gaaacagctg gtaataacca aataaagtca
     1321 atgtaaaaac cacgtatgta aaaaatttaa gatattttaa taattcctgg ggattatctt
     1381 taaatgtcag ataaagataa attcctaaaa ctactatggt tgcgaaaaaa cccagcaaaa
     1441 agatgctcgc ttgtatattt tttgaaaaaa ctaaagatag tgctcttgtg aaaaatatta
     1501 ttaaaagagt cagaatgtag ggatctttga tgtaaggaag aagggatttg atgcccccgc
     1561 tcttaactac ataaagatac gtcttagtaa ttcctgcaaa taacagagct aacataaata
     1621 tccttgccgg catcaaatcc cacctataga aactaaaaag ctccttgtgc atcaaaacag
     1681 acaacactat caaaacgtaa agtgaaaact taaaattctt caaaacaaga atataagaag
     1741 aaacaaatat taaaaatatt actacgagat ataaataatt cattttttct tttttgaact
     1801 ttttggtttt ttattgatgg tctcaccgac ctcaggtttc atcgatgaaa gaacatcttc
     1861 atagacacta agagtctttt cggctgcgtt ctgccaagta tattcttttc ttatcttttc
     1921 gtccaaaacc ggtcggggtt caaaatattc ttttttaata gcttctgtta ttgattcgac
     1981 attatcagga acacaaaaag acgcaaaact tccgagatat tctttagctc gaggccccgc
     2041 tgcaactgta ttggctccgc agtaaagtgc ttccaaactg gttaaacctg ttgtctcaaa
     2101 ccagctggct gaaacgcaga ccttagcgaa atggtaatag gaaggaagat cagagtaatt
     2161 tatcgaatta tccaatctgg taatccattt atgttttctt agcaacttat ttattaaaaa
     2221 attatattcg aagtgtttca tggggctggc ttttccgatt aataccagct tagcatcaag
     2281 tccggtttct tctctaaatt ttttaacccc ttttataaca ttaagttggt tttttctggg
     2341 ctcaattctg ccaacgcaca taatataatc ttcaaattca aatggattct taaaaggctt
     2401 tggcttaata aaagcttccc ccactccgtt taataccttc tcccacttaa aagttacatc
     2461 aaatgttctt tttaaatctt ctgcttctct tacagtttgg actaaaactg cgtcggatat
     2521 ttccaaaact tctctttgca tttttttgaa tccgtgaaaa acactgtata acgtaggttt
     2581 aaattttctc ctatcaaata aagatctgta aacattttta aaggtatctc ttctgaattg
     2641 atctctaaaa aataatttcg aaattcttct gaaatcgaat acaaactcat catcaaattt
     2701 tttgacttct ttcacatcgt gatgaatagg agataaaact atcggaacac ctgctttttt
     2761 cactttttta gcataaaaat aggtttcggg agtccaatct agttgaaaaa tgtggaccaa
     2821 atcgtaatct ttaggttcaa aatccatatc cgttttaata tctacatcaa taccaagttt
     2881 ttttaattcc gaggctgtgt tttctacctg aaccctgtcc ccgccccggg cgtccaacag
     2941 accgattcgc cctaacatta aaactttcat tttttatctc ccattagttt taaatacaaa
     3001 taatcatact tatctacaaa actctccaat gaataattat tctcaacaaa ctctctcgcc
     3061 ccttccacca aatttttatt ttcactacct gttttattgt actcatcaac catcttatct
     3121 gacaaatcct cgtaatcgcc aacattaaaa aatttaacat agtcttctcc gacttctttt
     3181 aaaacttcaa ggttggaaca aatcacaggc aatcccatac tcatagcctc tataagaaca
     3241 attccaaaac cttcggcaag tgtcggaaac acgaaaacat caaatgtaga aaggtatttt
     3301 atcttatctt cgtcatcaaa tcttcccgta attacaacat ttttatccaa ttccaattct
     3361 ttgactaaat ttttaatttt atcttccaaa gcaccgcccc cgacgattaa taaatacatt
     3421 ttctctttac ggaaaagctg tgaatttaga aattttgaga atgcacgcac taatacttcg
     3481 tgtccttttt cttgagtaat tctacctaga tttccaaata caaacgcatc ttcgggaatc
     3541 ccgtgtcttc ttttaatttc tttttcgaat tcaaacctat gcaaagccgg tatattaaaa
     3601 attgaggtat caattccgtt aggaatcact tccaaaattt tctcgtttat tccctctttg
     3661 attttaactt tcttctttga ttcggtaagt gctatttccg cgcttgataa tttatttacg
     3721 acataagaat acaacttaac attaaggaat tttttaattg aattaatttg ccactcagat
     3781 attggggtgt gagtatgtgt tacaacagct ttaacttttg tatttctgca agcaagtaag
     3841 gaattaacta cagctttaag ttcatgagaa tgaacgatat ctatattatt ctcttcaaca
     3901 tattttttaa gattggctat atatttaaaa tcaaagtctt ttgaaatgaa ttcatttgaa
     3961 actactgcac catttttttg atataaatca gacatgtctc cttgaggaca ccaaacaaaa
     4021 acctcgtgtc ctttttttac catccccttc acaagatcga gaacgtgaac ttccatccct
     4081 ccgaacttcg atgaatttaa aacatagagt attttcattt tatattctcg ttaatataac
     4141 taagcatggc ggcaatttta ccctcccaag tgtttttctt agcaacttcc attctttttt
     4201 tagacttctc agcgatgtct tcttctaaag ctcttttaat atttagagaa aattcttccg
     4261 gatttttgga gatataacaa acatcctcaa aaggataata agagggaaga tcagtgacca
     4321 ccaagggaat cccggcagcc aaaggttcaa aaaatttcac aggaaaacat ccgccgactg
     4381 tgtaatcatt caactgatat gggattgtca taacgtcaaa gaacgaaata tattttggaa
     4441 tttcagtaaa gtcttttcta cctaagaaat aaacattatc caatttctca aaacccaagc
     4501 tttttttact tgattcttta tcttttaatg ccaaaggccc tataactaca aatgaaaatg
     4561 acgggtaatc atccgcaatt tttttaaaca attccctatc aaacttgtac tcatcaacag
     4621 caccggtaaa acccactatc gggtgcggaa tattttttaa atcttcggga acttcaaaac
     4681 ctttgttttt tacatcaaaa aatctttcaa aatcgccgac attgggagtg aaatatgttt
     4741 tcggattaat ttttttaagt ttttcagcca aaccgggagt ggtagtgaaa acaatatttg
     4801 ctcgagaggt taacagacgc tcctgatcaa caatccatct tttcttttca ggagtattgt
     4861 attttggaaa tccttcataa ttatctacac aatcataaat caaaaaatca tgtttaatat
     4921 tttttaaata attttctaaa ccttctatct ctacgtgata aatccataaa atagtttttt
     4981 catttccgct atctttaaaa actttttgag atatctcatt tattttctga gagtgtttag
     5041 ccgaagaaat accttttaat gggtgggtat ttaaaggtga ataaaccaca actccttctt
     5101 cagttttttt cgttttagta aaaagtcgtt ttaaagacca gtttctctgc aaaatttgtt
     5161 tggcgaatac cctgcctgta ttaataggtg ggtctatgaa taaaacacta tgcccgagct
     5221 ttggtaaccg gctcataaca tgtcttttat tagtccatag gggaaaatcc caaagctggt
     5281 ttgaaagaca gattatgttg tattttggtt cttcgatagt cattttagag ggctgagccc
     5341 gcgggctcag cctagcaatc tcattataca ttacagaagg gctgagcccg cctgcgggct
     5401 cagcccggga aaggcccggc ccacttgtgg gccgggcccc aacttacttc agcggaagac
     5461 atttcgggca agctctttca agcattttag cttttactaa caaccttctg acattaaaca
     5521 taaactcaga atcttttagg gaccaaagcc aagatgtatt tttaaatccg gtgtcgtaag
     5581 cggcgtgcca aattccgtca aaaaacacta taccgaaagc ctgaactaat attgaaaaac
     5641 caaacaaaag agaaaacaaa aaatgttttt tatcatacca ggaagatttt agataaggca
     5701 ccattaaaag aacaagataa ggaataacat ccgagctcat tctgtaacca aaggaataac
     5761 caccgtacca gtgtttccaa aaactaatta ctaaggtgtg aattaccaca atcagaaagt
     5821 aaaaaagata ttgaagattc tcttttaatc ctttttttac agctatataa aaaccgacaa
     5881 atgaaaaaag gaaaaccggg gagtacgtta gaattccttt gctgggagac aaccatactc
     5941 caaaaaaact aaccggaaaa ggagagatcc aacttccgaa caattggctt gcataaccct
     6001 gattttcaat cccaatatag tatttgttgt tataccaaag aaaaaatgca acgtttaaaa
     6061 gaaatcccaa agctacaaaa acacctgttt tcaatataga tttaaggtta tttaatttta
     6121 tgagagcata aattccgatg aaaagaagtc cgagtgctgc ggtgggtctt gacagaaatg
     6181 ctaaaccgta aaaaagacca ctcaaaaacg cgctaaaagg cttgaaattt gggcttttca
     6241 ggtggtccag aacaaagtac aaacccagga ttgaaaaaag ctgtaaagtg ccgtgttgcc
     6301 acaaagactg agacaacatc gcaaggttta ctgttgcgaa caaaaaaaca taagttagaa
     6361 cagaagcctt tttctcatca agggaaaatt gtttttttaa taaaagatag aaaaatcctc
     6421 ctgataaagc cataatcaat gctgaagata tttttgaaag aataatgagg ttttcccatg
     6481 ttaccaccat tccaaatttt aatggtaaaa aataaaccgg aaccgccagt aaaccactta
     6541 ttaaagtaaa cgcggaaaca taatgtccat caattttctt taaataaaaa ggagtaagac
     6601 ctcggatttg atttttatca tcggggtggg gaaatttact aatcagcata ttgtagtagc
     6661 taccaagata tagagttttc tcttttatta tcgctacagg taaaaacata gcgggaacgg
     6721 tgtcttctga ttgaattgct aatttattga tatttgtttt gtaaactaat aaccaagacc
     6781 cgaagtatat gaaaaacgat ataaaaacaa tctgaaaaaa tttctttgaa ttcatgggtt
     6841 tattttacac gattttggag ggctcagccc ggaacgggct gagcctagga tgggctgagc
     6901 ccggaacggg ctcagccctc cctcacggta atctcaccgg aagatatttc ttagtggacc
     6961 aaattaaaaa tctatctttt aactttccct cttttattag cccgtttata ggcacagata
     7021 tatcaaattt accttcttca tcaaaattaa gatcaaaacc caaatcataa gaatcaccaa
     7081 aaatataatt agttacaggt acaattgcag atgtagttaa accacccgaa tcaattattt
     7141 cgtatcccct gccttttgaa aaatactcag gtaaataaac ctgatcccca ttaataattt
     7201 caatatcttt aatatcacta ctattaacat caactaatct tataaataaa aattcagaat
     7261 ccgtaaaagg tatttccaat ttaactttat aagtctcggg ctcccctatt tttgaacaac
     7321 cagggaaata aagaagagtt ttatcatcat gacctactac cccttcttct aaataataaa
     7381 aatcaacctt tattttatcg gtcccaatat ctggcctttt acatgaaaag gttatttgaa
     7441 tttcaggttc ggaactcccg aattcctttt taagattttt aatttgaaag aaatttgaag
     7501 gctcctcaga tcttagttca ttagaaagat tctcgctgga attaattgcc aaaacaatat
     7561 cttccaaatc accattttga atatagaaaa ccccttcatt tttaaaatct ttagcatttt
     7621 tgttttcaac tcttgaattg ttgggtgtat aaaccaaata attatcaaca aacactggct
     7681 cagag
//
LOCUS       DAOTFA010000017         7267 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate AMR_MDS_3115
            NODE_7295_length_7267_cov_6.492374, whole genome shotgun sequence.
ACCESSION   DAOTFA010000017 DAOTFA010000000
VERSION     DAOTFA010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868139
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (wastewater metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7267)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7267)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:36:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 536
            CDSs (total)                      :: 521
            Genes (coding)                    :: 520
            CDSs (with protein)               :: 520
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7267
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7295_length_7267_cov_6.492374"
                     /isolate="AMR_MDS_3115"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="P5014_01115"
     CDS             <1..807
                     /locus_tag="P5014_01115"
                     /inference="COORDINATES: protein motif:HMM:NF014839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HRY56742.1"
                     /translation="KDMESFNNWGYVFNLQKGIKNNNYNLKLIFTPSQDLLFLPEKAR
                     AIDFAYVVNQKLGDNSVGVIINGEKKDLGYILKNADTVEVITGERNTKEDIKLEKICL
                     RKTHEYLESKIIDNEKKNSIELGKKIIEDRVSPRGILDLEDIGTDVHGICYSLGCENA
                     GEIYYKISKGYLDIERLDDLLNEYGVTKNRQKRTTIETAGIDKPGLLNIITKSVNKSG
                     GNIVRIVFNKDKNNVHFYLRIVVENLKLKDTEILRKELTNNKYFHKIKIV"
     gene            complement(1124..1852)
                     /locus_tag="P5014_01120"
     CDS             complement(1124..1852)
                     /locus_tag="P5014_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56743.1"
                     /translation="MRIEFKIDPHYDYEMTIQMLRGKDWEYRAKKMSLDMDLVRRINT
                     CEGKELKQAREELENLVTNLYKDLGFAIEKAKDTYQKSWDSIIDEFSSTVEEITVPWF
                     YDKYIVNVTHFNRGLSNWDGNVVGRWWREDADKQRRITAHEVLLAHFYSIHRKLYANS
                     GLDKEQIWALSEIFAFALTGLEPSISKFWPWDTKGYYTDHNYPQIVDLQNALKEPYLN
                     RKSFDEYVTKGIELAKELFPISRH"
     gene            1987..2613
                     /locus_tag="P5014_01125"
     CDS             1987..2613
                     /locus_tag="P5014_01125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56744.1"
                     /translation="MAAPITHIALTEKIFNNKFHNYDKKEFIIGTSFSDIRYLGTIDR
                     EKTHLSVNNLEDINDKNSFISGMKFHALVDKVREDFLLSRNIYSFCPESKYLTQSIKF
                     VEDKILYEKISDWPVYQNYLDNILQDEIESGAGEETVTQWHNLLKNYFANKPNEESIR
                     NFVVKINLPENVAEEIINNVNVIEQIPEVNSYIKDLYNDFEGLLNLDN"
     gene            2723..3436
                     /gene="pyrH"
                     /locus_tag="P5014_01130"
     CDS             2723..3436
                     /gene="pyrH"
                     /locus_tag="P5014_01130"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002659291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HRY56745.1"
                     /translation="MAEYKRVLLKISGEVFGGQKKVGLDFKAIGKIASNIKNLIDETG
                     IQLAIVNGGGNLFRGRNYREGTVDPAQMDYIGMLGTIMNALALQQELNALGVQSRVMS
                     SLRLDQACEPFIRLRAIHHMEKGYVLILAGGTGSPFFTTDSAAALKAAEINADILLKG
                     TNVDGVYDCDPNQNSNAKKFTHLTFQDALVKGLMVMDSTAFALCQSQNIPLVVFDVRD
                     FKNVKRVIKGEKVGTLITP"
     assembly_gap    3604..3703
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3704..5086
                     /locus_tag="P5014_01135"
     CDS             <3704..5086
                     /locus_tag="P5014_01135"
                     /inference="COORDINATES: protein motif:HMM:NF012882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-Tu/IF-2/RF-3 family GTPase"
                     /protein_id="HRY56746.1"
                     /translation="SDDQLDFPVLYCIGREGKSWNQITSDILSSENSESRTNSDLSPI
                     FEAILKYIPSPKINNEEPFQMLVSTLDWDSFKGKYAIGKVTRGIAKVGMTVTLLKANG
                     EKEDTVIEKIYVTRGLNRVEVQEANSGEIIAITGIKNAGIGDTVADSNQPEALPTIAI
                     DEPTLSISVGPNTSPFMGREGRFFTSRQILERIQQELQTNVAMRFKNSNEEGFVISGR
                     GELHLSVFLETLRREGFELSAGKPTVITKIIDGVEMEPVEELTVDVDNVYVGAVKSEV
                     GQRHGILLSQDELTENSSRLLFEISTRGMLGLRGTLLTLTKGTAVTNSNFLKYEKMGP
                     QMKKMRKGVLIAAYSGNAAPHGLITAQTKGPVFISPSEKVYEGMIVGLNGRDEDIEIN
                     VCKEKQLTNNRSSGEGTSVALIPPVIMSIEQCLGFLENDELLEITPRSLRLRKKILNS
                     TLRMRASRKN"
     gene            5151..5546
                     /locus_tag="P5014_01140"
     CDS             5151..5546
                     /locus_tag="P5014_01140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56747.1"
                     /translation="MNKKYIALGSVGLLLGGLLLFTNKVSAYKGDPTVKGPYYTEERH
                     EEMTKAFDSNDYNAWKEQMGGRGRVSQVINEENFERFAEAHRLALEGKTEEAAKIRQE
                     LGLGLQNGSGKGMGQGFGGCEECPRNLNR"
     gene            5611..6156
                     /locus_tag="P5014_01145"
     CDS             5611..6156
                     /locus_tag="P5014_01145"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRY56748.1"
                     /translation="MENIETKTDEEIVEIVKNENKESYAHIIKRYQAKLIRYAEYLIG
                     DPDKASDAVQDSFIKAYVNLNGFDVKKKFSSWIYRIVHNEAINLIDKNKKTIKLDPDV
                     DFDSGINIEDTYIKEELVSKAHNCLEGMDINYREPLSLYYIENKSYEEISDILRLPMG
                     TVAVRINRAKIILKKICQTKR"
     gene            6168..6650
                     /locus_tag="P5014_01150"
     CDS             6168..6650
                     /locus_tag="P5014_01150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56749.1"
                     /translation="MEKQKRNIENKIMDKVLSGEVKMKPKWYFVLGSITSFLGLVGMI
                     IGAIFLTNLTFFFIKKQGPGTARINMMLESFPLWVPILAVAFIILGIWMLKKYDFSYK
                     KNFIVVAVTVVTAIILSALIIDLLGLNEIWAKRGPMRRFYMEDSNYRVQPLHRPMNRR
                     "
     gene            6687..>7267
                     /locus_tag="P5014_01155"
     CDS             6687..>7267
                     /locus_tag="P5014_01155"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRY56750.1"
                     /translation="MNISLVSPKSKTEVQDAYNFVKNILKFSEDHPRSLDFYFSVFQK
                     DPKYTVIAKNGNEIVGTIISSDENEDTLLIGELAVNEKYRKLGIGTKLIQQLEQNAIE
                     IGKKEITLGAQGPAENFYLKMGYTPRLFIQLTGNNRIDEIKKYIPNKVVWENTGDGFS
                     KVIVETNGVDKQLQKNISEKTGAHTQYLFSKRIV"
BASE COUNT         2712 a         1117 c         1314 g         2024 t
ORIGIN      
        1 aaagatatgg aaagttttaa taactgggga tatgttttca atctacaaaa aggaatcaaa
       61 aacaacaact acaacctaaa attaatattc acaccctcac aagatttatt gttccttcct
      121 gaaaaagcta gagcaataga ttttgcttat gtagtaaatc aaaaattagg cgataactcg
      181 gtgggtgtga taataaacgg agagaaaaag gatctaggct acatactaaa aaatgcagat
      241 acagtagagg ttataacagg cgaaagaaac actaaggagg atattaaact agaaaaaatt
      301 tgtttgagaa aaacccacga atatttagaa agtaaaataa tagacaacga aaagaaaaac
      361 tctatcgagt taggtaaaaa aataattgaa gacagggtat ctccaagagg aatattggac
      421 ttagaggaca ttgggacaga tgtgcacggt atttgttatt cgctaggttg cgagaatgct
      481 ggagagattt attacaaaat atcaaaaggg tatttagata ttgaaaggct agacgatttg
      541 ctaaatgaat atggtgtaac aaaaaacaga cagaaacgaa ctacaataga aactgcagga
      601 atagacaaac caggactttt aaatattatt actaagtcag tcaataagag tggaggaaat
      661 attgttcgta ttgtttttaa taaagataaa aacaatgttc atttttatct tagaattgtt
      721 gtagaaaatc taaaattaaa agacacagaa atcctacgaa aagagttaac gaataataaa
      781 tatttccata aaattaaaat tgtttaaaat tttgaatttt ttttaactaa ctaaaattaa
      841 aacagcaaac atcggttcgc caacagcata cgtggtaaag tagttctcac atcctcacaa
      901 atggcccacc atgaaagttc ccgttagcct tgaatttatg aaaggttagc gggaactttt
      961 attatgagag aggacgagaa tgtcggactg agcatagcga aggagacggg gtcgcgagct
     1021 ttagagacac ttatgaattt atgaactagt gtgtcttaag acgtgtgacc gagtcctctc
     1081 tcgctcacca aaaacattgt atttgttgtc acagcaaggt aatctagtgt ctagagattg
     1141 gaaataattc tttggctaac tcaatacctt tagtaacata ttcatcaaaa gattttctat
     1201 ttaggtaagg ttcttttaat gcattttgca aatctacaat ctgtgggtaa ttatgatctg
     1261 tataataacc ttttgtatcc caaggccaaa atttacttat actaggttct aaccctgtca
     1321 gagcaaaggc gaatatctca gagagagccc atatttgttc cttgtctaaa ccactgtttg
     1381 cataaagttt tctatgtatc gaataaaaat gggcaagaag tacttcgtga gctgtaattc
     1441 gtctttgttt gtccgcatct tctctccacc atctaccaac tacattccca tcccaattac
     1501 ttaaacctct gttaaagtgg gtaacgttaa caatatattt atcgtaaaac caaggaacag
     1561 ttatctcctc tacggtactt gaaaactcat caatgatcga atcccaagat ttttgataag
     1621 tgtccttagc tttttcaatt gcaaaaccaa gatctttata taaattggta acaagatttt
     1681 ctaactcttc cctcgcctgt ttaagttctt ttccttcgca agtatttatt ctccgaacca
     1741 aatccatatc caaactcatc tttttagctc tatattccca atccttacca cgtaacattt
     1801 gtatcgtcat ttcgtaatcg tagtgcggat ctattttgaa ctcaattctc ataatataat
     1861 tttacactag caacactaca gaatttctca catccttaca aatggcccac cacatttttt
     1921 tatcgatata acaaccttat ttttactata cccagagaag cctagaactt ttgatacaat
     1981 taagttatgg cagccccaat aactcacata gctctaacag aaaaaatatt taacaataaa
     2041 tttcacaatt acgataaaaa ggaatttata atcggaacta gtttctcaga tataagatac
     2101 ctagggacaa tagatagaga gaaaactcat cttagtgtaa ataacttaga agatataaac
     2161 gataaaaatt ctttcatttc aggaatgaag tttcacgctt tagtagataa agtcagagaa
     2221 gactttttac tttcaagaaa tatctattca ttctgtccgg aatctaaata ccttactcaa
     2281 tcaataaaat ttgttgagga taaaattctt tacgagaaaa tatctgactg gcctgtttac
     2341 caaaattatt tagacaatat attacaagat gaaattgaat cgggcgcggg ggaagaaact
     2401 gttactcaat ggcacaattt actaaagaac tattttgcaa acaaacccaa tgaggaaagt
     2461 attcgtaact ttgttgtaaa gattaactta cctgaaaatg tggctgaaga gataattaat
     2521 aacgtgaatg ttatcgaaca gatacctgaa gttaattctt acattaaaga tttgtacaac
     2581 gattttgaag gtttactgaa cctagataac taatccccac accttcacaa acgccctttc
     2641 aacaatacgt atcagattgt aaactactac gtttttgaat gtatctttca acttaaacat
     2701 taaaaagata taataatcaa caatggcgga atataaacgc gtattgttaa aaataagtgg
     2761 agaagttttt ggaggacaga aaaaagttgg tttggatttc aaagctattg gaaaaattgc
     2821 atccaatatc aaaaatctca tagacgaaac aggcattcaa cttgcgattg ttaacggcgg
     2881 aggtaacttg tttagaggaa gaaattatcg tgagggaact gtagacccag ctcaaatgga
     2941 ttatataggt atgttgggaa caatcatgaa cgctttagca cttcagcaag aattaaacgc
     3001 tcttggggtt caatcacgag taatgtcatc tttaagacta gatcaagcat gtgaaccttt
     3061 tatacgttta agagctattc atcacatgga aaaaggatat gtactgatat tagccggagg
     3121 aacggggagt ccttttttca caacagactc ggctgccgca ctcaaagctg cagaaataaa
     3181 cgcagatatt cttcttaaag gtacaaacgt agacggggtt tatgactgcg acccgaatca
     3241 aaattcaaat gcaaaaaagt tcacacacct cacatttcaa gatgctttag tgaaaggttt
     3301 aatggtcatg gattctactg cattcgcatt atgccaatct caaaatattc ctttggtagt
     3361 ttttgatgtc agagatttta aaaatgttaa gcgtgttatc aaaggtgaaa aagtaggtac
     3421 actgattact ccttaacgaa taatattctt tagaaatttt gaaactttct aaatgcttat
     3481 aaccatcctt tatatttcca tgcaaaaaat tagtcgttaa gccaatgtag atgtataatc
     3541 accaattgct atgaaactaa gaaatattgc cataattgca catgtcgatc acggaaaaac
     3601 aacnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnagcgacg atcagttaga
     3721 ttttccagtt ctttactgta taggaagaga aggaaaatct tggaaccaaa ttactagcga
     3781 catactttca tctgaaaatt cagaatcaag aacaaactcc gatctgtcac ctatttttga
     3841 agcaatatta aaatacatcc cgtctccaaa aataaataac gaggaaccat ttcaaatgct
     3901 tgtaagcact cttgattggg atagctttaa gggtaaatac gcaataggaa aagtaacaag
     3961 aggaattgca aaagttggta tgacagtaac tttattaaaa gcaaatggtg agaaagaaga
     4021 tacagtaatt gaaaaaattt atgtgacccg aggacttaat agagtggaag tacaggaggc
     4081 taattctggt gaaataattg ccattacggg aattaaaaat gcagggatag gtgacactgt
     4141 ggcagattca aatcaacctg aggccttgcc tactattgca atagacgaac caacattaag
     4201 tatatcggtc ggtccaaaca cgtctccctt tatgggaaga gagggaagat tttttaccag
     4261 cagacaaatt ttagaaagaa ttcaacaaga actacaaact aatgtggcaa tgagatttaa
     4321 aaattcaaac gaagagggtt ttgtaatctc aggaagaggg gaacttcatc tctctgtatt
     4381 tttagaaaca ttaagaagag aggggtttga acttagcgca ggaaaaccga cagtaataac
     4441 aaaaattatt gatggggtgg aaatggaacc tgtagaagaa ctcacagtag atgtagacaa
     4501 tgtttatgtt ggagcagtga aaagtgaagt tggtcaaaga catggaatat tgctttcaca
     4561 ggatgagcta acagaaaatt cttcaagact tctttttgaa ataagtacca gaggaatgtt
     4621 gggattaaga ggcacccttc taacactaac aaaaggaacc gcggtaacta attcaaactt
     4681 tttaaaatat gaaaaaatgg gtccgcagat gaaaaaaatg agaaaaggag ttttaatagc
     4741 cgcgtattca ggaaatgcag ccccacacgg gcttataacc gcacaaacaa aaggtcctgt
     4801 tttcatctct ccttcagaaa aagtttacga aggaatgata gtggggctta acggaagaga
     4861 cgaagacatt gaaataaacg tgtgtaaaga aaagcaacta acaaacaaca gatcatcggg
     4921 ggaggggaca tccgtagctt taatacctcc agtcatcatg agcatagaac aatgtcttgg
     4981 attcttggaa aatgatgaat tacttgaaat tacccctcgt agcctcagat taaggaaaaa
     5041 aatccttaac tcaactctaa gaatgagagc ctccagaaaa aattgaaaga aaatctcctc
     5101 ctcacctgta ctagatatgt acgagttaat aattaaggag gtgaaattaa atgaataaaa
     5161 aatatattgc attaggttca gttggtcttt tattaggagg tttgctactt tttacaaaca
     5221 aagtatcagc atacaaagga gatccaacag ttaagggacc ttattacaca gaagaaagac
     5281 atgaggaaat gaccaaagct ttcgatagca acgactacaa tgcttggaaa gagcaaatgg
     5341 gtgggagggg cagagtatca caggtaataa acgaggaaaa tttcgaaaga tttgcagaag
     5401 cacacagact tgctttagaa ggcaaaactg aagaagcagc taagataaga caggaattag
     5461 gtttaggact ccaaaacggt tcaggaaaag gaatgggtca gggattcgga ggatgtgaag
     5521 aatgcccaag aaatctcaat cgttaataaa tcatttacaa atgcctcatc taaaatctag
     5581 gtggggcatg atgtaaaatg atagggcact atggaaaata ttgaaacaaa aacagacgag
     5641 gaaatagtgg agattgttaa aaatgaaaac aaagagtcgt atgcacatat tattaaaaga
     5701 tatcaagcta aacttattag atacgcagag tatttaattg gcgatccaga caaagcaagc
     5761 gatgcagttc aagattcttt tataaaagca tacgtaaatc tcaatggttt tgatgttaaa
     5821 aaaaagtttt caagttggat ttacagaatt gttcataacg aagctataaa tttgattgat
     5881 aaaaacaaga aaactataaa actcgaccct gacgtagatt ttgatagtgg gataaatatt
     5941 gaagatacat atataaaaga ggagttagtt tcaaaggcac acaactgtct tgagggaatg
     6001 gatattaact accgtgagcc actatctctt tactatatag aaaataaatc atacgaagaa
     6061 ataagtgata ttttaagatt accaatgggt acagttgcag ttagaataaa tagggcgaaa
     6121 attattttga aaaaaatatg tcaaacaaaa agataggaaa tttaaaaatg gaaaaacaaa
     6181 aaagaaatat cgaaaataaa ataatggata aagtattatc cggagaagta aaaatgaaac
     6241 caaaatggta ttttgtgtta ggttccataa cttcatttct gggattggtt ggaatgataa
     6301 tcggagcgat atttttaact aatctaactt tcttttttat taaaaaacaa ggcccgggaa
     6361 cagcaagaat taatatgatg ttagagtcat ttcctttgtg ggtaccaata ctagcagttg
     6421 cttttatcat cttgggtatc tggatgttaa aaaaatatga tttttcatat aaaaagaatt
     6481 ttattgttgt tgcagtaacg gttgtaacag caataattct atctgcacta attattgatc
     6541 ttttaggatt aaacgaaata tgggctaaaa gaggaccgat gagaagattt tacatggaag
     6601 attccaacta tagagtacaa ccactacaca ggccgatgaa cagaagataa ataggttggt
     6661 tatagcctta ctgatataat ttatctatga atatctcttt agtatcacca aaatctaaaa
     6721 cagaagttca agacgcatat aactttgtta aaaacatact taaattctct gaggaccacc
     6781 ctcgaagttt agatttttac tttagcgttt ttcaaaaaga tcccaagtat accgtaattg
     6841 caaaaaacgg aaatgaaata gttgggacta taatatcttc agatgaaaac gaagacacac
     6901 ttttgatagg agagttagca gtaaatgaaa aatacagaaa attaggaatt ggaacaaaac
     6961 ttattcaaca acttgaacaa aatgctatag aaataggaaa aaaagagatt actcttggcg
     7021 ctcaaggacc tgccgaaaat ttttatttaa aaatgggtta tacaccaaga ttatttatcc
     7081 aacttacagg aaataacaga atagatgaaa ttaaaaaata tattccgaat aaggtagttt
     7141 gggaaaatac cggtgacggg tttagtaaag taattgttga aaccaacgga gtggacaaac
     7201 aactacaaaa aaatatttca gaaaaaacag gtgcacatac gcagtatttg ttttctaaaa
     7261 gaattgt
//
LOCUS       DAOTFA010000018         7047 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate AMR_MDS_3115
            NODE_7623_length_7047_cov_6.413330, whole genome shotgun sequence.
ACCESSION   DAOTFA010000018 DAOTFA010000000
VERSION     DAOTFA010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868139
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (wastewater metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 7047)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7047)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:36:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 536
            CDSs (total)                      :: 521
            Genes (coding)                    :: 520
            CDSs (with protein)               :: 520
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7047
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7623_length_7047_cov_6.413330"
                     /isolate="AMR_MDS_3115"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..272)
                     /locus_tag="P5014_01160"
     CDS             complement(<1..272)
                     /locus_tag="P5014_01160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56751.1"
                     /translation="MNEEDMQLEQRHLGIVLSKIEQAKNTINESLDKLGKENIERLKE
                     LSEDGSSSRGYGSNDFDVLLEQLHQKNLSFNLKGKYQTLNELKFLSS"
     gene            complement(269..955)
                     /gene="rsmI"
                     /locus_tag="P5014_01165"
     CDS             complement(269..955)
                     /gene="rsmI"
                     /locus_tag="P5014_01165"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HRY56752.1"
                     /translation="MEKGKLYIIASPIGNLSDFTLRGIETLKSVDLILSEDTRETDKV
                     LKKYEINIPQISYRDQNHEVVFPKIEASLENGLDLALISDSGTPLISDPGYKLVRDLI
                     KKGFEVVSIPGPCAAISALASSGFPTDKFTFLGFLPKTSGDRSRLIKKYGDLDSTLIL
                     YESPFRIEKLLKEIQENLGERYVCLANEITKVHEKILRGNVSLLIGLVKGKHLKGEFV
                     VLVGKEGFSI"
     gene            complement(1043..1219)
                     /gene="rpmG"
                     /locus_tag="P5014_01170"
     CDS             complement(1043..1219)
                     /gene="rpmG"
                     /locus_tag="P5014_01170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002556991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HRY56753.1"
                     /translation="MAKKKGNRILIGLECTETGKRTYVTQKNRMNSEGKLEVMKYNPL
                     LKKHTLHKEVQKLK"
     gene            1315..3804
                     /gene="leuS"
                     /locus_tag="P5014_01175"
     CDS             1315..3804
                     /gene="leuS"
                     /locus_tag="P5014_01175"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012160425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HRY56754.1"
                     /translation="MKEYDHKKIEEKWKNIWLEDNVYEAFDFSDKPKKYILAEFPYPS
                     GNSLHAGHMMRYTAPDVYARYLRMKGYNVLFPMGWDAFGLPAENYAIKTGINPAVITH
                     DTIKKFKESFLKMGYGIDWNREIDTSSPEYYKWTQWIFLKFFENGLAEYKETPIWWCE
                     DLKTVLADEEVITDKEGNKISERGEKPVIKKMLKQWVLKIPAYAEKLIEGLDKVDFPE
                     SIKLAQKNWIGKSEGANIIFDIDGEKIEIFTTRPDTVFGVTFMVFAPEHPFIKKIIDK
                     AENKEEVNSYIEKSKNKSDLERQIQKDKTGVILKGIEATVPFTNKKVPVFIADYVLMD
                     YGTGVIMAVPAHDERDYEFAKKFNLEIVEVIKPENEEKKEVQISNEKRDSKEPILYTG
                     EGVMVNSDKYSGMKSMDFKNKVVEDLEKDGKGKKEVNYKIRDWVFSRQRYWGEPIPLV
                     HTPEGVKAICNTENPDEVNKYLPLTLPEIADFRPSDDGASPLERNSDWVNTNFDGQPA
                     RRETNTMPNWAGSCWYYLRYTDPKNENEFASKDKMNYWLPVDKYFGGAEHTTVHLLYS
                     RFWHKFLYDLKLVPKEEPYSWRLNGGLLLTQQGTKMSKSSENGVDPITEIDKYGADSL
                     RTTICFMGPYDETYPWNPNIIKTINKLIKNIISLQEKVCDKPSDEETLKSYNSMLKNI
                     TNMIENLKMNTCVSEIMIFIKNIQNNNYIGKDVWLGFLKVLSVFAPFISEDLWQEINN
                     YSEWKKENSIHLQPWPLVEEKYLEIDTVVIPVQINGKVRTEILISKSDTEDIVKEKAL
                     SDEKIQVLLRDKNIQKFVYIKDRIANIVLKP"
     gene            complement(4161..4913)
                     /locus_tag="P5014_01180"
     CDS             complement(4161..4913)
                     /locus_tag="P5014_01180"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HRY56755.1"
                     /translation="MTEKNTPTKKNKKLNIVYIIIGVLLIGALGYGIYSYKFSTKRTI
                     TVSGTSTGDYANQISTFYINLEYHNKEKDKAVQELNEKSKVAVDKIKEFGIDSKDIMT
                     QNLNVYQREDPYYEDGITKYKPNEWYASYSIEITLRDLTKSTDFTEFLTSIESSSMWG
                     PNLSIDSKKTDSDELLTSAIEDARQKAERMAKSMGAKLGKVIKVEEGGAGSPILYGLA
                     KEDSRMMGGGGAPIEPGSTEISKTVTVTFELR"
     gene            4963..5724
                     /gene="map"
                     /locus_tag="P5014_01185"
     CDS             4963..5724
                     /gene="map"
                     /locus_tag="P5014_01185"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HRY56756.1"
                     /translation="MQKGLIKTEEEIIFMRKLGKTLSGIFGELEIIITPGIKAYDLED
                     KFLELCNKEKVIPSCKGYKEGLLPPFPTGLCMSINQQSVHCFPRKGVTLKEGDIVNID
                     TVISSNGLHVDSAHCYHVGKTTDKRLKLIETSKNALYSAISKVKASTKIGSISQTLQK
                     TVEKEGFNVLKDYEGHGIGYSMHEFPEIPCYGNKHDGPKLKAGMTICIESLICEGDDQ
                     VINTSEWETEMADGKDFCIFEHTVLVTKNGFEILT"
     gene            5744..6289
                     /locus_tag="P5014_01190"
     CDS             5744..6289
                     /locus_tag="P5014_01190"
                     /inference="COORDINATES: protein motif:HMM:NF016214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HRY56757.1"
                     /translation="MRNKLVEKSQTYHILIHILLGLICALIISREFQGENLFLLLICG
                     FLGNTLPDLDHVIYYLTYGKDSEYSQIIKLFIKERDFKEVKDFLRENHKYLTGLYSHT
                     ILSPLITGFLTYFFYTKNKIYLATLFLSITVHFSYDILEDLLFFKRLNPNWFMHFNKK
                     NKNNQGIQLPYLEFLKSKKKN"
     gene            complement(6286..>7047)
                     /locus_tag="P5014_01195"
     CDS             complement(6286..>7047)
                     /locus_tag="P5014_01195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56758.1"
                     /translation="CCEDMQNFENYKYPVQGVYYALYFNLRNEKLDDVELRRKMLKVL
                     PIEDLVYYYGIPVQGPISRSTFTDKSLPFDIYDENFEDDLEKLEIKVNVPDEKNHVKL
                     AENVLKMWEDKLNLEVTIVKTDPKEFVDKVIETRDFEVLLYGQEVGRDPDRYVLWHSA
                     QKEPPNLNLSGFEQIRADRALEEGRNELDNDKRVVHYNEFQKVLNEQVPAIFLYHPYI
                     NHYVSKYIEGIGQKYTFTYWDRFLDFKNWNKISSN"
BASE COUNT         2406 a         1150 c         1166 g         2325 t
ORIGIN      
        1 gatgaaagaa atttaagttc atttaatgtc tggtatttac cttttaaatt aaacgataag
       61 tttttttgat gaagttgttc caaaagaaca tcaaaatcgt ttgatccata acctctggag
      121 ctggaaccgt cttcactcaa ttctttcagt ctctctatat tttcttttcc aagtttatct
      181 aagctctcat ttatagtgtt tttggcttgt tcaattttac tcaaaacaat accgagatgt
      241 ctttgctcga gttgcatatc ttcttcattc atatagaaaa cccctccttt cctactaaca
      301 ctacaaactc accttttaga tgtttacctt ttacaagccc tattaataag cttacatttc
      361 ctcgtaatat tttttcatgt actttggtaa tttcatttgc taaacaaacg tatctttcac
      421 caaggttttc ctgaatttct tttaacaact tctctattct gaagggagac tcatataaaa
      481 ttaatgttga gtctaaatct ccgtattttt ttataagccg tgatctatca cctgatgttt
      541 ttggcagaaa acctaaaaat gtaaatttat ctgtaggaaa tcccgaagag gcgagtgcag
      601 aaatcgctgc acaaggacct gggatagaaa ctacctcaaa tcccttcttt attaaatccc
      661 taactaattt gtatccagga tctgaaataa gcggagtacc gctatcagaa attaatgcta
      721 aatctaagcc attttcaaga gatgcttcaa ttttaggaaa tactacctcg tgattctggt
      781 ctctataaga tatttgagga atattaattt catatttttt aagtacctta tcagtttctc
      841 gtgtatcttc ggatagaatt aaatcaacac tcttaagtgt ttcaattcct cttaatgtaa
      901 agtcggagag gtttcctatg ggagatgcaa ttatatagag cttacctttt tccatatttt
      961 ttagtaatgc ccagtacgaa catctattct atcacttttt tagttaattt gcgttaaatc
     1021 ttgaattagg gaaagtataa atttacttaa gtttttgaac ttctttatgc aaagtatgtt
     1081 tctttaataa agggttgtac ttcataacct caagtttacc ttctgaattc atacgattct
     1141 tttgagttac gtatgttctt ttaccggtct cagtgcactc aagacctatt aatattctat
     1201 ttcctttttt cttagccata gcaagttaat atagtataaa aataatactt ttgcaatgcg
     1261 caaatatcta taatgctgag catagcgggc tgtgataaaa tcaaggtgtt aaatatgaag
     1321 gaatacgacc ataaaaaaat agaagaaaaa tggaaaaata tatggctcga agacaatgtt
     1381 tatgaagcct ttgatttttc agataagccg aaaaaatata tcttggcgga atttccctat
     1441 ccttccggta attctttaca cgcaggacat atgatgagat acacagcgcc cgatgtttac
     1501 gccagatacc ttagaatgaa gggttataac gtacttttcc ctatgggttg ggatgctttt
     1561 ggacttcctg cagagaatta tgctattaaa actggaataa atcctgcggt aataacgcac
     1621 gatactataa agaaattcaa agagtcgttt ttaaaaatgg ggtacggaat tgactggaat
     1681 agggagatag atacaagtag tccggaatat tataaatgga cgcaatggat tttcttaaaa
     1741 ttttttgaaa atgggttggc tgaatataaa gaaactccta tttggtggtg cgaagattta
     1801 aaaacggtat tggcagacga agaagttatt acagataaag agggaaataa aatatcggaa
     1861 agaggtgaga aacctgttat taaaaaaatg ttaaaacaat gggttcttaa aattcccgct
     1921 tacgcagaaa aattaattga aggtttggat aaagttgatt ttcccgaatc aataaaatta
     1981 gcacagaaga attggattgg aaaaagcgag ggcgcaaaca ttatttttga tattgatggg
     2041 gagaaaattg agatttttac tacaaggcct gataccgtgt tcggggttac attcatggtg
     2101 tttgcgcccg agcatccttt tattaaaaaa ataatagata aggctgaaaa taaagaagag
     2161 gtaaatagct atattgaaaa atcaaagaat aaatctgacc ttgagagaca gatccaaaaa
     2221 gataaaactg gggtcatatt aaaaggtatt gaagccacgg ttccttttac aaataaaaag
     2281 gttcctgttt ttatagctga ctacgttctt atggattacg gtacaggtgt cattatggca
     2341 gttccggcac atgacgaaag agattatgaa tttgctaaga aatttaatct tgagattgtt
     2401 gaagttatta aacctgaaaa tgaagagaag aaagaagttc agattagtaa tgagaaaaga
     2461 gattctaaag aaccaattct atataccggt gaaggggtta tggtaaatag tgataaatat
     2521 tcaggaatga aatctatgga ttttaaaaat aaagtagttg aggatttgga gaaagacgga
     2581 aaagggaaga aagaagttaa ttataaaatt agggattggg ttttttccag acaaagatat
     2641 tggggagaac ctataccttt ggtacacaca ccagagggag tgaaagcaat ttgtaatact
     2701 gaaaatcccg atgaggtaaa taagtatctc cctctcaccc ttccggaaat agctgatttc
     2761 agaccatcag atgacggagc cagcccactt gaacgaaact ctgactgggt caacacaaac
     2821 tttgatggtc aaccagcacg tagagaaact aacaccatgc ccaactgggc tggctcctgc
     2881 tggtattatt tacgttacac tgatcccaag aatgaaaacg aattcgcaag taaagacaaa
     2941 atgaactatt ggctacctgt agataaatat ttcggtggtg ccgaacacac tactgtacat
     3001 cttctctact ccagattttg gcataaattt ttatatgatc taaaattagt cccgaaagaa
     3061 gaaccatatt catggagact taacggaggt cttctattaa cacagcaagg tactaaaatg
     3121 tctaaatctt cagaaaatgg tgttgatcct attacagaaa ttgacaagta tggtgcagat
     3181 tctttaagaa ctaccatttg ttttatgggt ccttacgacg agacataccc atggaatcca
     3241 aatattatta aaactattaa taagttgata aaaaatatta tttcccttca agagaaagtt
     3301 tgtgataaac ccagcgatga agaaacatta aagtcatata actcaatgtt aaaaaacatc
     3361 acaaatatga tagagaattt aaaaatgaac acctgtgtta gtgaaataat gatttttatt
     3421 aaaaatattc agaataataa ttacatcggt aaggacgtgt ggttaggatt tttaaaagta
     3481 ctatcagttt ttgctccttt catttcagaa gatttgtggc aggaaattaa caattactct
     3541 gagtggaaaa aggaaaacag cattcatctt caaccatggc ctttagtaga agaaaagtat
     3601 cttgaaatag acactgtcgt aattcctgtt caaataaacg gaaaggtacg aacggaaatt
     3661 ctaataagca agagtgatac agaagatatc gtaaaagaaa aagcactaag cgatgaaaaa
     3721 attcaggtat tacttaggga taaaaacata caaaaatttg tatatataaa agatagaata
     3781 gctaatattg tattaaaacc ataaagaatt tatttatctt tatctttact ttattaaatt
     3841 tttttatttt attgacttat tttaatattt gacaaattaa ttggtatatt ataggatata
     3901 cgcagtaccg ctggcacgga ccaaatctcg gtttgcaaaa atatgagcca caaaccctca
     3961 tatgtcagtt ctttatagag tgtccataga aatctaatcg gacccaccgc cgttagaaat
     4021 ctcaaacatc taataaatgg tactttttta ttatgaaagg gcaacggtgg aagacggtaa
     4081 ttttgatgga aaaaaactat ggatcctagt tttttacacc aagttaccgt atttttttaa
     4141 atatttctgg taatttaaat ctatcgtagt tcaaaagtca ccgtaactgt ttttgatatt
     4201 tctgtagaac ctggttctat aggagctcca ccaccaccca tcattcgtga atcttccttt
     4261 gctaatccat acaatatagg ggaacccgca ccaccttctt ctactttaat cactttgcct
     4321 aatttcgcac ccatgctttt tgccattctt tcagcttttt gtcttgcatc ctcaattgct
     4381 gaagtaagta actcatccga atctgttttt ttagagtcta tagataaatt aggaccccac
     4441 atacttgaac tctctataga ggttaaaaat tcagtaaaat cagttgattt tgttaaatct
     4501 cttaaagtaa tttcaattga atagcttgca taccattcat taggcttata tttggtaatt
     4561 ccatcttcat aatagggatc ttctcgttga taaacgttta aattctgagt cataatatct
     4621 tttgaatcaa taccaaattc ttttatttta tctacagcaa cttttgattt ttcattcaat
     4681 tcttgaacgg ctttatcctt ttccttatta tgatattcaa gatttatata aaaggtagag
     4741 atctgatttg cataatctcc tgtcgaggtc ccggatactg ttattgttct tttagttgaa
     4801 aatttataag aataaatccc ataacctaag gcgcctatta gcagaacacc tataattata
     4861 tagacaatat taagtttttt atttttcttt gtaggggtat ttttttccgt catacatata
     4921 tattatcaca aataaaaatc aatatagagt ataatcactc ctatgcaaaa aggacttata
     4981 aaaactgaag aagagattat atttatgaga aagttgggaa aaactctctc tggtattttt
     5041 ggagaactag aaataattat cacccctgga ataaaagcat atgatttgga agataagttt
     5101 ttagaattgt gtaataaaga aaaggtaatc ccttcatgca aaggttacaa agaaggtctt
     5161 ttacctcctt ttccaaccgg cctttgtatg agtattaatc aacaatctgt acactgtttc
     5221 ccaagaaaag gtgtaactct taaggaagga gatatcgtca acattgatac agtaatttcg
     5281 tctaatggtt tacacgtaga cagcgcacat tgctaccacg tgggtaaaac aacagataaa
     5341 aggttaaaat taatagaaac atctaaaaat gcactttatt ctgcaatatc aaaggtaaaa
     5401 gccagcacaa aaattggttc aatctctcaa acgttacaaa agacagtaga aaaggagggc
     5461 tttaatgtat taaaagatta cgaaggtcac ggcataggtt actctatgca cgaatttccg
     5521 gaaataccat gttacggaaa taaacacgat ggtccaaaat taaaagctgg tatgactatt
     5581 tgtatagagt ctttaatatg cgagggagat gatcaagtta ttaatacaag tgagtgggaa
     5641 accgaaatgg cagacggcaa ggatttttgc atttttgaac acactgtttt agtcacaaag
     5701 aatggtttcg aaatactaac ttaactaaac aagagataca aatatgagaa ataagctggt
     5761 agaaaaaagt caaacttatc acattttaat ccatatacta cttgggttga tttgtgctct
     5821 aattatttcc agggaatttc agggagaaaa tctttttctg cttttaattt gtggtttttt
     5881 aggaaataca cttccagacc tagaccatgt catttattac cttacatacg gaaaagacag
     5941 cgagtattca cagattatta aattatttat taaagagagg gattttaaag aggtaaagga
     6001 tttcttaaga gaaaatcata aatatctaac aggcttatac tcacacacta tcttgagtcc
     6061 tttaattaca ggctttttaa catatttttt ctatactaaa aacaaaatat atcttgcgac
     6121 cctcttttta tctatcacag ttcatttctc atacgacatt ttagaagatc tccttttttt
     6181 caaaaggtta aatccgaatt ggtttatgca ttttaataag aaaaacaaaa acaatcaggg
     6241 aattcaatta ccatacttag aatttttaaa aagtaaaaag aaaaattaat tggatgatat
     6301 tttattccag tttttgaaat caagaaatct atcccagtat gtaaatgtat atttctgccc
     6361 tatgccttct atgtattttg aaacataatg atttatatac gggtgataaa ggaatattgc
     6421 agggacttgc tcatttaata ctttctgaaa ttcattgtag tgcacaaccc ttttatcgtt
     6481 atccagttcg tttctgcctt cctctaatgc tctgtcggct cttatttgtt caaaaccgga
     6541 taggtttaaa ttcggtggtt ctttttgagc agaatgccac agtacgtatc tatcaggatc
     6601 tctgcctact tcctgaccat acaataatac ttcaaaatct ctcgtttcga ttactttatc
     6661 aacaaactcc ttggggtcag ttttaacgat agtgacttcc aaattcaatt tatcctccca
     6721 catttttaaa acattctccg cgagttttac atgatttttt tcgtcgggga cattcacttt
     6781 tatctccaat ttttccaaat catcttcaaa attttcatcg tatatatcaa agggtaagct
     6841 tttatctgta aatgtgctcc tacttatagg cccttgaact ggaataccat aatagtagac
     6901 caaatcttct ataggtaaca cttttaacat ttttcttctt aattctacat cgtccagttt
     6961 ttcatttcta aggttaaaat aaagagcata gtacactccc tgaactggat atttataatt
     7021 ttcaaaattc tgcatatctt cgcaaca
//
LOCUS       DAOTFA010000019         6883 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate AMR_MDS_3115
            NODE_7860_length_6883_cov_6.022115, whole genome shotgun sequence.
ACCESSION   DAOTFA010000019 DAOTFA010000000
VERSION     DAOTFA010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868139
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (wastewater metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6883)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6883)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:36:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 536
            CDSs (total)                      :: 521
            Genes (coding)                    :: 520
            CDSs (with protein)               :: 520
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6883
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7860_length_6883_cov_6.022115"
                     /isolate="AMR_MDS_3115"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..501
                     /locus_tag="P5014_01200"
     CDS             <1..501
                     /locus_tag="P5014_01200"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HRY56759.1"
                     /translation="SIAVYTILQRSEVSSNLARLDGIRYGNDRKSFGFEAKKRIMLGT
                     YTLSAGYYDAYYSKAQKVRTLIKNDFEEAFKKIDLIVAPSMPCIAPELGQSEKSSMYG
                     ELMDLLSEPSSVAGLPGISIPCGFSQGMPVGLQFIGNIFGEKDIIEVSNLFQEGTDFH
                     KDFPSL"
     gene            511..894
                     /locus_tag="P5014_01205"
     CDS             511..894
                     /locus_tag="P5014_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56760.1"
                     /translation="MCLFNLFKKSIKRKIITVETREKVQRDWKNVEILLSQKGPSQLR
                     QALITADKSLDNALKDIFDGETMGERLKNAESFFERDQYNRIWEAHKVRNNLVHESGY
                     EPPYFVVTKAVQDLKEGLEKLGVRV"
     gene            914..>1254
                     /locus_tag="P5014_01210"
     CDS             914..>1254
                     /locus_tag="P5014_01210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit B"
                     /protein_id="HRY56761.1"
                     /translation="MEKVTRDNYKLVLGLEIHLHLRTKRKMFCRCSADIWEKEANSIT
                     CPVCLGLPGALPVPNEDAVKKTQLLGLALGCSLNQNSKFDRKHYFYPDLPKGYQISQY
                     KEPLCLGGQVEL"
     assembly_gap    1255..1354
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1355..2098
                     /locus_tag="P5014_01215"
     CDS             <1355..2098
                     /locus_tag="P5014_01215"
                     /inference="COORDINATES: protein motif:HMM:NF014930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56762.1"
                     /translation="MRLEANISLRTQEMEKKGELCNYKVEVKNINSFRFMEKAVLAEI
                     DRQLQVFEKGEMPIQENRGYNETTGKTVPQRTKEDAKDYRYFPEPDIPPMVFSNGYIN
                     ELKKELPELPHMIKKRLVQNYGIKISESIVLVDNFGMEIVNKFEEFIKKGLDSKKMAS
                     ALINKKETHLWTFDKFKDYLQVPDELIDENALRDLIIKVMENNPKAVEDYKNGKESSI
                     QFLFGQIMRETGGKVKPDYAIPLIKSLIQ"
     gene            2165..3994
                     /locus_tag="P5014_01220"
     CDS             2165..3994
                     /locus_tag="P5014_01220"
                     /inference="COORDINATES: protein motif:HMM:NF012688.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HRY56763.1"
                     /translation="MELKFDPENPIYVHVTTESQYNEALEDLEKQKVVGVDIEGTSLD
                     PYSSILLTVQIGTSEKSYIFDARALKLAEMPRIKSFLEDPKIIKILHNGKFDYKHIKQ
                     NLHISMCNIFDTMLTETILNAGLSSSYYSLKDLAVKYTGVTLQKSVRESFEYMTASTK
                     LNESQLKYGAVDTLILFPIFEEQIKKLKKEQLVDIAKLEYATTRVVGDMELKGVHIDV
                     KRWRQLINDLAKKRDELAKLFQQDIRPLFKSNTVDLFGNIGDSININSQVQLMDLFNN
                     KMHLNLPSTGDGILELCDNPTVKILREYRGYEKLVSAFGENLLSKINPITKRIHPDFN
                     QLGAATGRFSCNNPNFQQIPRQSENAPFRSCFNPEPGYKLVTTDYSTFEMRIMAELSG
                     DANLVDAFKRGVDVHSHTAALMFGLEMTDDFAKKYPNERFAAKSINFGLMYGRGPTSL
                     AKQLGISPERGREYLDKYFKSYPGVQKYLDRVSKEAVKKGWSTTPAGRKRWYAQPDPT
                     DPDYTRKISHIQRQAKNHPIQGTNADAIKYALVFLTEKIKEKNIDASIVLTVHDEVVC
                     EVREDQAEEWSVIQSNEMMRAGQLFIKKVPVESKPFVSDVWEH"
     gene            4066..>5090
                     /locus_tag="P5014_01225"
     CDS             4066..>5090
                     /locus_tag="P5014_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018307180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRY56764.1"
                     /translation="MVLCHRCGQRPARIPITRIVDGKQVYMALCEVCYEEVMQETTET
                     ASKLEKFGRDLTQMARQGKLDPVIGRKDEIDRVIHILSRRTKNNPVLIGDPGVGKTAI
                     VEGLAQKIVEGLVPEPLRGKRVFMLDMASIVAGTSHRGMFEERLKDIIQEVITASGQI
                     ILFLDELHTVVGAGSAEGSMDAANILKPALSRGELQMVGATTIDDYRKYIERDAALER
                     RFQTIMVDEPTPDETIEILEGLRERYEKHHHVKILDEAIETAVKMSDRYISERFLPDK
                     AIDLIDEACALVRLSQVKEPENLKTLEEEIEDLNKKIKKKDLLIDEKNKLVAKVENLE
                     KVKAELL"
     assembly_gap    5091..5190
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <5191..6033
                     /locus_tag="P5014_01230"
     CDS             <5191..6033
                     /locus_tag="P5014_01230"
                     /inference="COORDINATES: protein motif:HMM:NF019344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRY56765.1"
                     /translation="GAFLFIGPTGVGKTELAKALAEALYGDEDMMVRLDMSEYMEKHS
                     VSKLIGAPPGYIGFDQGGQLTDIVRRKPFSVILLDEIEKAHPDTFNALLQIMEDGRLT
                     DSKGRTVDFKNTILIMTSNVGSDFLRRQEIGFDKSSKKEKDESDKEVSTRVTEILKNS
                     FRPEFLNRIDEIVMFNNLSQDDTKEITRRLVEKTKKLLMEQGVSFELNESAITYLAEK
                     GYSEEYGARPLRRLIQKELDNVLSDRIIRGDLIKGEKVLVKADKEGLVIDVDKKVKVK
                     ALKK"
     gene            6265..6633
                     /gene="rplS"
                     /locus_tag="P5014_01235"
     CDS             6265..6633
                     /gene="rplS"
                     /locus_tag="P5014_01235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014624706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HRY56766.1"
                     /translation="MSEELEGVEEVKKDEERIPDFGVGDTIRVSYKIIEGDKVRTQPF
                     QGIVIAKKGSDVSKTFTVRKIGADGIGVERIFPFLSPNIEKVKVMKKGKVRRAKLYYL
                     RDRVGRKATKIKEKVVQTAK"
     gene            6693..6848
                     /locus_tag="P5014_01240"
     CDS             6693..6848
                     /locus_tag="P5014_01240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56767.1"
                     /translation="MKILKSLLKPLFFVALAFVICTIWIIKNIRPMELINDIVKDKTN
                     SYEKDTI"
BASE COUNT         2527 a          936 c         1381 g         1839 t
ORIGIN      
        1 tccatagctg tttacacaat tctacaaaga tccgaggttt catctaactt agccagactt
       61 gacggtataa gatacggtaa cgatcgaaaa agctttggat ttgaagctaa aaaaagaata
      121 atgcttggaa cgtacacttt aagcgcaggg tattatgatg cctattattc aaaagctcag
      181 aaagtgagaa ccctgataaa aaatgatttt gaagaagctt ttaaaaaaat tgatttaatt
      241 gttgctccat ctatgccgtg tattgctcct gaactcggtc aaagtgaaaa atcatcgatg
      301 tacggagaac ttatggatct tttatcagaa cccagcagtg tggcaggttt accgggaatc
      361 tctattccct gcggttttag tcaagggatg cctgtgggat tacaattcat cggtaatatt
      421 tttggcgaaa aagacataat agaagtatcc aatttatttc aagaagggac tgattttcat
      481 aaagattttc catctttatg atttataaat atgtgtttat ttaatttatt taaaaaaagc
      541 attaaaagaa aaataattac agttgaaaca agagagaaag ttcagagaga ttggaaaaat
      601 gttgaaattc tcttatccca aaaaggtccg tcacaactta ggcaagctct tattactgcg
      661 gataaatcct tggataacgc tttaaaagac atttttgacg gagaaacgat gggggagagg
      721 ttaaaaaatg cagagagttt ttttgaaaga gatcagtaca atagaatctg ggaagctcac
      781 aaggtaagaa ataatctggt tcatgagtct gggtacgaac ctccttattt cgttgttacc
      841 aaagcagtcc aagatttaaa agaaggttta gaaaaattag gggttcgggt ttaatttaaa
      901 aaatttattt aatatggaaa aagtaacaag agacaactac aaattagttt taggtcttga
      961 aatacacttg catttaagaa caaaaagaaa aatgttttgc aggtgtagtg ctgatatatg
     1021 ggaaaaagaa gctaattcaa tcacatgtcc ggtgtgttta ggccttccgg gagcacttcc
     1081 ggttcccaac gaagacgctg tcaaaaaaac acaattacta ggtttagctc ttggttgtag
     1141 tctaaaccaa aactctaaat ttgatagaaa acattacttt tatcccgatc ttcccaaggg
     1201 atatcagata tcacaatata aagaaccact gtgcttgggt ggtcaagtag aactnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnatgaga cttgaggcta atatttcact
     1381 tagaacgcaa gaaatggaga aaaaaggaga gctgtgtaat tataaggtgg aagtaaaaaa
     1441 cattaattca tttaggttta tggaaaaggc ggtccttgct gaaatagata ggcagttgca
     1501 ggtttttgaa aaaggagaga tgcccataca agagaatagg ggttacaatg aaactacagg
     1561 aaaaacagtt ccccaaagaa ctaaagaaga cgccaaagat tacaggtact ttcccgaacc
     1621 tgatattccc ccgatggttt ttagcaacgg ttatataaac gagttaaaaa aagaacttcc
     1681 tgaacttccc cacatgataa aaaagagatt agttcagaat tatggaataa aaatttcaga
     1741 atctattgtt ttggttgata attttggtat ggagatagta aataaatttg aagagtttat
     1801 aaaaaagggt ttagattcta aaaaaatggc aagtgcttta ataaacaaaa aagaaaccca
     1861 cttatggaca tttgataaat ttaaagatta tttacaggtt ccagatgaat taattgatga
     1921 aaatgccctt agagatttaa ttataaaggt gatggagaat aaccctaaag cagtagaaga
     1981 ttacaaaaat ggtaaagaga gctctattca gtttttgttt ggtcagataa tgagagaaac
     2041 tggaggaaaa gtaaaacctg attatgcaat acctcttata aagagcttaa tacagtaaaa
     2101 agtatcaaat accctaacct ctgtttgtaa atttatttga tattaactgt taatataact
     2161 tcgtatggaa ttgaaatttg atccagaaaa tccaatatat gtccacgtga caacagaaag
     2221 tcagtacaac gaagcgttgg aagatctcga aaaacaaaaa gttgttgggg tcgatattga
     2281 aggtacttcc ctagacccat acagcagtat tttgttaacc gttcaaattg gaacttcgga
     2341 gaaatcctat attttcgatg ccagagccct taaattggct gaaatgcctc gtataaaatc
     2401 atttctagaa gatccaaaga taataaagat actccacaat ggaaaatttg actataaaca
     2461 cataaaacaa aatttacata tatcaatgtg taacattttt gacacgatgc ttaccgaaac
     2521 aatattaaat gccggtttat caagtagtta ttacagttta aaagatttag ccgttaaata
     2581 cactggagta actcttcaaa aaagtgttag agaatctttc gaatacatga ctgcgtcaac
     2641 aaaattaaat gaatcccagc taaaatacgg tgcggttgat acattaattc tatttcctat
     2701 ttttgaggaa caaatcaaga aactaaaaaa agaacaatta gtggacatcg caaagcttga
     2761 atatgctaca acaagagtag taggggatat ggaattaaaa ggagttcata tagatgtaaa
     2821 aagatggaga caattgatta acgacctcgc aaaaaaaaga gacgagttag caaaattatt
     2881 tcaacaggat atacgacctc tgtttaaatc aaatactgtg gacctttttg gcaatattgg
     2941 ggactcaata aacattaata gtcaggttca gcttatggat ctttttaata ataagatgca
     3001 tttaaacctt ccctctacgg gagatggtat tttagaactt tgtgataatc ctacagttaa
     3061 aattttaaga gaataccgag gctacgaaaa attagtatct gcttttggtg agaatctact
     3121 ttcaaaaata aaccccatca ctaaaagaat tcatcctgat tttaaccagt tgggcgcagc
     3181 aacaggaaga ttttcttgta ataatcctaa ttttcaacaa atacctagac agtctgaaaa
     3241 tgcacctttt agatcttgtt ttaatcctga acctggctac aaactagtaa ccacagatta
     3301 ctctacattt gaaatgagaa ttatggcgga gttatctgga gacgccaact tagtggatgc
     3361 ctttaaaagg ggtgtagatg tacattctca cactgcggca ctgatgttcg ggcttgaaat
     3421 gacagacgac tttgcaaaaa aataccctaa tgaaagattt gccgccaaat caattaattt
     3481 tggcttgatg tacggaaggg gtccgacaag tttagctaaa cagcttggta tatcacccga
     3541 aagagggcgt gagtatcttg ataaatactt taagtcatac ccaggagttc agaaatatct
     3601 tgatagagta tcaaaggaag cggtaaaaaa gggttggagc actactccag ctgggagaaa
     3661 aagatggtac gctcagccag atcctacaga tcctgattac acaagaaaga tctcgcatat
     3721 tcagaggcaa gcaaagaatc accctattca gggaaccaat gctgacgcta ttaaatatgc
     3781 cttggtgttt ttaactgaaa aaattaagga aaagaatatt gatgcctcaa ttgttttgac
     3841 tgtacacgat gaggtcgttt gtgaggtcag agaagatcaa gccgaggaat ggtcggtaat
     3901 acaatcaaat gagatgatga gggcaggaca attatttatt aaaaaagttc ctgtagaatc
     3961 aaaacctttt gtaagcgatg tgtgggaaca ttaagggtat ttgttattat ttataggtag
     4021 aattgggtct tgcactctaa tgtatagagt gctaatatct acaatatggt tttatgtcac
     4081 agatgcgggc agagacccgc gagaatacca ataacaagaa tagttgatgg aaaacaagtc
     4141 tatatggccc tttgtgaggt ctgttacgaa gaagttatgc aggaaacaac cgaaacagct
     4201 tccaagcttg aaaaattcgg tagagattta acccagatgg caagacaagg gaaattagac
     4261 cctgttatag gaagaaaaga tgaaatagac agagtaatac acatcttatc ccgaagaact
     4321 aagaataacc ccgttttaat aggagacccc ggtgtaggta aaactgcaat agttgaaggt
     4381 ttagctcaga aaatcgtgga aggtctggta ccggagccac taagaggaaa aagagttttt
     4441 atgctcgaca tggcttcaat agtcgcagga acttcacata gaggaatgtt tgaggagaga
     4501 cttaaagaca taattcaaga ggtaataaca gcttcagggc agataatcct gtttttagat
     4561 gaattacata cagtcgtggg agctggaagt gccgaaggtt ctatggatgc cgcgaatatt
     4621 ctaaaaccgg ctttatcaag aggagaactt caaatggtgg gagccactac aatagatgat
     4681 tacagaaaat atattgaaag agacgcggct ttggagagaa gatttcaaac aataatggtt
     4741 gatgagccaa cacctgacga gactatagaa atcttagagg gtttgagaga aagatatgag
     4801 aaacaccatc atgtaaaaat attagatgag gctatagaga cagcagtaaa aatgagcgac
     4861 agatacatta gtgaaagatt tttacctgac aaggcgattg atcttataga tgaggcatgc
     4921 gccttagtta gattatctca agtaaaagag ccggaaaatt taaaaactct tgaagaagaa
     4981 attgaagact taaataaaaa aataaaaaag aaagatttac tcattgatga gaaaaataag
     5041 cttgtagcta aagtagaaaa tcttgaaaag gttaaagccg agcttttaga nnnnnnnnnn
     5101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5161 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ggagcatttt tatttatagg acctacaggg
     5221 gttggtaaaa cagagttagc aaaagcatta gcggaagctt tatatggaga tgaggatatg
     5281 atggttagat tggatatgag tgagtatatg gaaaaacact ctgtgagtaa acttatcggg
     5341 gctcctccgg gatacattgg atttgatcaa ggtggtcagc taacagatat agttcgaaga
     5401 aaaccatttt cagtaatatt acttgatgag attgaaaaag cgcatcccga tacatttaat
     5461 gcattgttac aaattatgga agacgggaga cttaccgata gcaaagggag gactgtagat
     5521 tttaaaaaca caattttaat aatgacctca aatgttggat cagatttttt aagacgacag
     5581 gagattggtt ttgataaaag ttccaaaaaa gaaaaagatg aatcagataa agaggtttcc
     5641 acaagggtta cagaaatatt aaaaaactct tttagacctg agtttttgaa tagaatcgac
     5701 gagatagtta tgtttaataa tttaagtcaa gatgatacca aagaaataac aagaagattg
     5761 gtagaaaaaa ctaaaaaact attgatggag caaggtgtgt catttgaatt aaacgaatca
     5821 gcgattactt acttagctga aaaggggtat agtgaggagt atggagccag acctttaaga
     5881 aggttgattc agaaagagtt ggataacgtt ttatcagata gaataatcag aggagatctg
     5941 attaaaggtg aaaaggtttt agttaaagct gataaagagg gattggttat agacgtagat
     6001 aaaaaggtaa aagtaaaagc tttaaagaaa taattgaatt tatttataaa atttgatact
     6061 cttccttagc ttatgtcaca agacgaaaac aaaaaaataa aaaaagatga aatagaagaa
     6121 aaagaagttg cagatgaagc tgttgtacct gaagaagttc aggaagaaaa ggttgaggaa
     6181 aaggctgttg atgtagaaga tatagataaa tccgatgaga tcaaagatat tgagaatgct
     6241 gaagatgagc ttgtagagga aaacatatca gaagaacttg aaggtgtgga agaggtaaaa
     6301 aaggatgaag agagaatacc tgactttggg gttggagata caattagagt aagttataaa
     6361 ataatcgaag gtgacaaggt aagaacacag ccatttcaag gtatagtaat tgcaaaaaaa
     6421 ggctccgatg tatcaaagac atttacagtt agaaaaattg gagcggacgg tataggtgta
     6481 gaaagaatat ttcctttcct ttctccgaat atagaaaaag taaaagtaat gaaaaaaggt
     6541 aaggtaagga gagctaaact atactatctg agagatagag ttggaagaaa agctactaag
     6601 attaaagaaa aagtagtaca gactgcaaaa taaagtagag cagcgtcctt tttaaatttg
     6661 aaaattcttc ctattatata gataataaac ccatgaaaat tctaaaatca ttactaaaac
     6721 ctctattttt tgttgcattg gcttttgtga tatgtactat ttggataata aaaaatataa
     6781 gaccgatgga gctgattaac gatatcgtaa aagataaaac aaatagttac gaaaaagata
     6841 ctatttaatt atttaaaaga atgctataat ccttttgttc tct
//
LOCUS       DAOTFA010000020         6795 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate AMR_MDS_3115
            NODE_8002_length_6795_cov_7.177448, whole genome shotgun sequence.
ACCESSION   DAOTFA010000020 DAOTFA010000000
VERSION     DAOTFA010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868139
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (wastewater metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6795)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6795)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:36:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 536
            CDSs (total)                      :: 521
            Genes (coding)                    :: 520
            CDSs (with protein)               :: 520
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6795
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8002_length_6795_cov_7.177448"
                     /isolate="AMR_MDS_3115"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="P5014_01245"
     CDS             complement(<1..305)
                     /locus_tag="P5014_01245"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRY56768.1"
                     /translation="MIVTKNLCKDYSGRDILIDVDIKINEGRKIGLVGKNGCGKSTLF
                     RLLNGIETPTSGNIEIQNEIIGYIPQEFSFPNEMVGVYLENKLDSKWDMYKIDLLVN"
     gene            377..634
                     /locus_tag="P5014_01250"
     CDS             377..634
                     /locus_tag="P5014_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56769.1"
                     /translation="MIQDIKAPITVYTAFNHKKNKAFPYFLKWDGIGYKIESINLHHT
                     YKSGFTLLHVFSVSTKTMSFKLVLNTDNLFWTVEQISDGIS"
     gene            681..1877
                     /locus_tag="P5014_01255"
     CDS             681..1877
                     /locus_tag="P5014_01255"
                     /inference="COORDINATES: protein motif:HMM:NF013015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56770.1"
                     /translation="MNSCFATVEQQADVMLRNKPIAVAAYKTDNGCIIAPSVEAKKLG
                     IKVGMRVKEGKMIYPELVVLEPDPWKYRNVHIGIKNILSTYTNDIAPKSIDEFVINLE
                     GFPAMKKGMEKVGLEIKERIKKEVGDYITVSIGIAPNRFLAKTAAGLTKPNGLDTIDS
                     TNHLEIFSKLQLQDLCGIKTNNTVRLNKAGIYSVLDFYNAPAKKLKMAFNSIIGYYWY
                     MRLHGWEPDDVISNRQSFGNSVALQGKYEKPEELSPILMKLIHKMSGRMRKAGYTAHG
                     VHLSLLYKNGSFWHQGKSVKEDLFSTGDIYKIAYKTLLHSPYRFPVHTIAVSCFNLSK
                     IKNSQLDFFDNIQKKKLLTDALDDISERWGDFVITPAMMLNTKDLVKDRISFGGVREL
                     EDLITI"
     gene            1942..2352
                     /locus_tag="P5014_01260"
     CDS             1942..2352
                     /locus_tag="P5014_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56771.1"
                     /translation="MRVYIGYKYRNFKDKEGLKVLLQKISDELSSLGHKTFILGRDEF
                     EWTHHKRPSKSILPIIKNMRKNDVLFAVIECESKSNGLFIESLFATILGKKVILAVKE
                     GVSPKPFSYFSKDVIEFYNYDNLLVSVKDRFKNL"
     gene            complement(2347..3561)
                     /locus_tag="P5014_01265"
     CDS             complement(2347..3561)
                     /locus_tag="P5014_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012995569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HRY56772.1"
                     /translation="MSHEPLAEKLRPSKIVDFVGQDKIIEVIKSLIEKSKTTDFFPSL
                     IFWGPPGSGKTTLARIISQELERPFFEFSAVNTSTKDIEKIIPKKKIPNEKGLGIIPE
                     SKETKTPIVFIDEIHRFNKAQQDSLLPHVEKGSIIFIGATTENPSFEVINPLMSRCRV
                     LILRQHEPKELEIILKRAEIMLGVEVDSDALDFLITASNGDARIGLNVLEISAQLSDT
                     KKISMKNVEDALQRRNLGFDMKGEEFYNTISALHKSMRGSDPDSALYWLGRMLEAGQD
                     PIYIARRVVRFASEDIGLEDPNALNLAVSAFKACEVIGMPECELALAEVVTYMAKAPK
                     SNQLYTAYGKVKEDVEKYGNLPVPLEIRNAPTKFMKEIGYGKNYKYEHSKEAKKTDQE
                     YMPEKLKGRKYL"
     assembly_gap    3668..3767
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3887..3962
                     /locus_tag="P5014_01270"
     tRNA            3887..3962
                     /locus_tag="P5014_01270"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3920..3922,aa:Lys,seq:ttt)
     gene            4070..4528
                     /locus_tag="P5014_01275"
     CDS             4070..4528
                     /locus_tag="P5014_01275"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRY56773.1"
                     /translation="MFPLDKGAPFIPNSNRKIQSLLNILIEMKDKYKFQKAIDLGSGD
                     GRIAIALTKEGFYCDGVELNKLLYKRSVGKIQKSGLGDKCKFFNEDFFKINLNKYDLI
                     VLWQSPQIMSKLSTKLKNELVKGTLICSYYFKIPHLDEMHKKDNWHIYEM"
     gene            4610..5353
                     /locus_tag="P5014_01280"
     CDS             4610..5353
                     /locus_tag="P5014_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008163168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRY56774.1"
                     /translation="MKLQNKTAIVTGASSGIGLGIAKLFLEEGANVVFSDINEEIGNN
                     IVSELGEKATFQKCDVSKSEEVYKLVEDTVGKYGKLDVMVNNAGIGGLGGILECTNED
                     WKKVIDINLSGVFYGTSAAAKAMKATQTKGSIINMTSILGIVGFQGAVAYSAAKGGVS
                     NLTRSSAQDLALSEIRVNAIAPGFIETNMTKGVLQDETFSNMIKNNTPLGYVGNTNDI
                     AQAALYLASDDSGYVTGIILPVDGGWTCR"
     gene            complement(5420..6169)
                     /locus_tag="P5014_01285"
     CDS             complement(5420..6169)
                     /locus_tag="P5014_01285"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRY56775.1"
                     /translation="MVLADYNDKNYDYTRYWDGRDYENASEYIALKSLLPSDYDESKK
                     VIDVGGGFGRLLPIFKQRFGSIYIFDYSKKLLETAEENASKLGLKINTVNGDVNEISS
                     LVSERFDFVTMIRVSHHLDDLEKVILQIKKILNKDGVFILEVANKIHFKSLISNIVKG
                     NFGYFKKDSVSVATKDVTFLNHHPKRVEEILKKCGFKIEKKLSVSNFRHQFFKKYIPI
                     KILLFLEKNMQKIGACVYFGPSIFYKVVNEE"
     gene            complement(6365..6640)
                     /locus_tag="P5014_01290"
     CDS             complement(6365..6640)
                     /locus_tag="P5014_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018692396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HRY56776.1"
                     /translation="MTKLYVGNLPYSVKDDALRDLFSSYGNVLSASIIFDRMSGRSKG
                     FGFVELEDDSEAERAISEMNEKELEGRKLVVSVARPKEERPERNFNR"
BASE COUNT         2210 a         1150 c         1102 g         2233 t
ORIGIN      
        1 ttgtttacca aaaggtcaat cttgtacata tcccatttgg aatccaactt attttcaaga
       61 tacactccaa ccatctcatt cggaaatgaa aattcctgtg gaatatatcc tatgatttca
      121 ttttgtattt ctatgtttcc ggaagtcgga gtctctattc catttaaaag tctaaataaa
      181 gtagatttac cacagccatt tttacccact aaaccaattt tcctaccttc gttgattttt
      241 atgtcaacat caataagaat atctctgccg gaatagtctt tacataggtt tttagtgact
      301 atcattggat aaatatacca cttgaaacta ttttaaacat agagtataaa atatctattc
      361 gggttttctt cgtattatga tacaagacat aaaagcacca ataacagtct atacagcctt
      421 taatcacaag aaaaacaagg cttttccata ttttttaaaa tgggatggga taggatacaa
      481 aatagaatcg ataaatttgc accataccta caaatcaggg tttacactcc tccacgtctt
      541 ctctgtttca actaaaacaa tgtcttttaa attagttcta aacaccgata atttattttg
      601 gacagttgag caaatatcag atggaatatc ttaaagacct gccctttaat ccaaagagtc
      661 caacaatcat gcatatagac atgaattcgt gttttgcaac cgtagagcaa caggcagatg
      721 taatgcttag aaacaaacct attgctgtag cggcttataa aacagataac ggatgcatta
      781 ttgctccctc ggttgaggca aaaaagctgg ggataaaagt aggaatgaga gtaaaagaag
      841 gaaaaatgat ctaccccgag cttgtggttc ttgaaccgga cccttggaaa tacagaaatg
      901 ttcatattgg aataaaaaac atcttatcaa cgtatacaaa cgatatagcc ccaaaatcta
      961 tagatgaatt cgtaattaac ttagagggtt ttccggcaat gaagaaaggt atggaaaaag
     1021 ttggtttaga aataaaagaa cgtattaaaa aggaagtcgg cgactatatt accgtttcta
     1081 tcggaattgc cccaaatagg ttcttagcaa aaacagccgc aggactaaca aaacccaatg
     1141 ggctagatac tatagattca accaatcact tagaaatatt ttcaaaacta caacttcaag
     1201 atttatgcgg tataaaaaca aacaacacgg tgagattaaa taaagcaggt atttattcgg
     1261 tcttggattt ttacaatgct cccgcaaaaa aattaaaaat ggcctttaac tcaattattg
     1321 gatactactg gtatatgaga ttgcacggtt gggaacccga tgacgttata tccaacagac
     1381 aaagctttgg gaactccgta gctttacaag gaaaatatga aaaacccgaa gaactgtccc
     1441 caatactcat gaagctaatc cataaaatgt caggaaggat gagaaaagct ggatatacag
     1501 cacacggggt tcatctatct cttttatata aaaacggatc tttttggcat caaggaaaaa
     1561 gtgtaaaaga agacttattt tcaactggag atatttacaa gattgcttac aaaacacttc
     1621 tccactctcc ttacagattt ccagtgcata caattgctgt atcttgtttt aacttatcaa
     1681 aaataaaaaa tagtcagctt gatttttttg acaatattca aaagaaaaaa cttctcacag
     1741 acgcgctgga cgacatttct gaaagatggg gagattttgt aattacacca gctatgatgt
     1801 tgaatacaaa agatttggta aaagacagaa tttcttttgg aggggttaga gagctggaag
     1861 atttaattac aatttaaata attaatttta atacactatc aaactttatt tgactatccc
     1921 aaaaatggta aaatatatgg aatgcgcgtc tacataggtt ataaatatcg aaattttaaa
     1981 gacaaagaag gattgaaggt tcttttgcaa aaaatatcag acgaactttc gtctttaggt
     2041 cataaaacgt ttatcttggg aagggacgag tttgaatgga ctcaccataa aagaccctcc
     2101 aaaagtattc ttccaattat aaaaaatatg agaaaaaacg atgttttatt tgcagttata
     2161 gagtgtgaaa gtaaaagcaa cggtttattc attgaatctt tatttgccac tattcttgga
     2221 aaaaaagtta ttctagctgt taaagagggt gtgagtccaa aacctttcag ttatttttca
     2281 aaagatgtga ttgaatttta taattacgat aatcttcttg tttcggttaa agaccgtttc
     2341 aagaatttat aaatattttc taccttttaa tttttcaggc atgtattcct gatctgtttt
     2401 tttagcttct ttgctgtgtt catatttata attcttaccg taaccaattt ctttcataaa
     2461 ttttgtaggg gcatttctga tttctaaggg aacaggtaaa ttaccgtatt tttcaacatc
     2521 ttccttcacc ttcccataag cggtataaag ttgattggat ttaggcgctt ttgccatata
     2581 cgtgactacc tctgctaaag ctagctcaca ttccggcatt ccaatgactt cacatgcctt
     2641 aaaggctgaa acagccaaat ttagggcatt tggatcctct aagcctatat cttcagaagc
     2701 aaacctaact acccttcttg cgatataaat tgggtcctgc ccagcttcaa gcattctccc
     2761 caaccaataa agtgctgaat cagggtcgga ccctctcata gacttatgta atgcagaaat
     2821 tgtgttataa aactcctcgc ctttcatatc aaaaccaagg ttccttcttt gtaaagcatc
     2881 ttcaacattt ttcatggata tttttttagt gtcgcttagt tgtgctgata tttctaaaac
     2941 atttagtcca atcctagcat ctccgtttga agcagtaatt aaaaaatcta aagcatctga
     3001 atcaacctca acccccagca ttatttcagc tctctttaat ataatttcca gttcctttgg
     3061 ctcgtgttgt ctaagaatca aaactcgaca tctagacatt aatggattta taacttcaaa
     3121 agaagggttt tccgttgttg cgccaataaa aataatactt cctttttcta cgtgcgggag
     3181 taacgaatcc tgctgtgctt tgttaaatct gtgaatctcg tcaataaata ctattggagt
     3241 tttggtttct ttactttcgg gtattattcc taaacctttt tcgtttggaa ttttcttttt
     3301 tggaattatt ttttcaatat cttttgtgga agtatttacg gctgagaatt caaaaaatgg
     3361 cctttcgagc tcttgagaaa ttatcctagc cagtgttgtt ttaccagatc ccggaggacc
     3421 ccaaaatatc aaagaaggaa aaaagtccgt ggttttggat ttttctatca aagatttaat
     3481 aacttcaatt attttgtctt gacctacaaa atctactatt ttcgaaggtc ttagtttttc
     3541 tgccaacggt tcgtgtgaca tagcttttta aattctacac tgttgggcac ttcgtgtaaa
     3601 cttcgggttc tggtatttta gtttgacaat atttttatct tttgctaatc tgttgttctt
     3661 gaaaattnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnaag agtgtgcctc
     3781 cccaccctac tcaatcgtac atagcatttt ttttatttcc tatgtaaatc tgtgtttaaa
     3841 aataatggtt gatagtttat aatatcgaag accgtaattt gtgtgagggg gcgtagctca
     3901 gtggttagag cacctgcctt ttaagcaggg tgtcgtgggt tcgatcccca ccgccctcac
     3961 caattttgaa taagggtatt atttgaaact ttttcaaggc aattaaaatt tacatatgca
     4021 tagtccattt ctcatattaa tatccgcttt aggttatttt cttttcttca tgtttcccct
     4081 tgataaaggt gctcctttta ttcccaacag taacagaaaa atacaatcac ttttaaatat
     4141 cttaatagaa atgaaagaca aatataaatt tcaaaaagct atagatttag gatctggaga
     4201 tggtcggata gccatagctt tgactaaaga agggttttat tgcgacggtg tagaacttaa
     4261 taaactactc tataaaagat cagttggaaa aattcaaaaa tcaggtcttg gcgataagtg
     4321 taaatttttt aacgaggatt tttttaaaat taatttaaat aagtacgatt taattgttct
     4381 ttggcaatct ccacaaataa tgagcaaact ttcgacaaag ttaaaaaacg aactagtaaa
     4441 gggcactttg atatgtagtt attactttaa aattcctcat ttagatgaga tgcataaaaa
     4501 agataactgg catatttacg agatgtaaca gctttattcg caattttcca atctgttcgt
     4561 aatttcataa tcattgtctg tatataaaaa cattgataag atttatctta tgaaattaca
     4621 aaataaaact gccatagtaa ccggtgcttc gagtgggatt ggtttaggaa ttgcaaaact
     4681 ttttcttgaa gaaggggcaa acgttgtttt ttccgatatt aatgaggaaa tcggcaataa
     4741 catagtttca gagcttggag aaaaagcaac ttttcaaaaa tgtgatgttt ccaaatcaga
     4801 ggaagtttat aaactagttg aagacacagt aggtaaatat ggaaaactgg atgtaatggt
     4861 aaataatgca ggaattggtg ggctgggagg gattttagag tgtacaaatg aagactggaa
     4921 aaaagtcatc gatatcaatc tatcaggagt tttttatgga accagcgcag ctgcaaaagc
     4981 tatgaaagcc acacaaacaa aaggttcgat tattaacatg acttcaattt taggaattgt
     5041 tgggtttcaa ggagctgtag catacagtgc cgctaaggga ggggtttcta atttaacaag
     5101 atcttctgcc caagacttag cgttgtcaga aataagagta aatgctattg ccccaggttt
     5161 tattgagaca aatatgacta aaggcgtttt acaagatgaa actttcagca atatgataaa
     5221 aaacaatacc cctttgggct atgttggtaa tactaacgac atcgctcaag ctgccttgta
     5281 tcttgcatct gatgattcgg ggtatgttac aggtataatt cttccggttg atggtgggtg
     5341 gacttgtaga tagtcttaaa catatccttt ttgaaatata ataaatatat tttcatcaac
     5401 ttgtattatt tgtaagaaat tattcttcgt ttactacctt gtagaaaata gaaggcccga
     5461 agtatacaca agcaccaatt ttttgcatat ttttttctaa aaatagcagg atcttgatag
     5521 gtatgtattt cttgaaaaat tgatgtctga aatttgaaac agaaagcttt ttttctattt
     5581 taaacccaca tttttttagt atctcctcta ctctttttgg atggtggttt aagaaagtta
     5641 catctttcgt ggctacactg actgaatctt tcttaaaata tccaaagtta cctttaacta
     5701 tgttacttat caaagattta aaatgtattt tatttgccac ctctaaaata aaaactccat
     5761 ctttattcag tatcttttta atttgtaaaa taaccttttc gagatcatct aaatgatggg
     5821 aaactcttat catcgtaacg aaatcaaacc tctcactcac caaggagcta atctcattta
     5881 catctccgtt gactgtattt atttttaggc ctaatttact tgcattctcc tccgcagttt
     5941 ctaaaagttt tttagaataa tcaaaaatat aaatagatcc gaatctttgt ttaaatatcg
     6001 gtaataatct accgaaacct ccacctacat caattacttt tttagactca tcataatcac
     6061 ttgggagtag actctttaaa gctatatatt cggatgcatt ttcataatcc ctgccatccc
     6121 aatatctagt gtagtcatag tttttatcgt tgtagtctgc aagaaccata cctcatatcg
     6181 tacattaatt acttaaaaat gaaaacaagg ttatataaat tgaaaatata gattagaaaa
     6241 tagtaaataa atatattgaa ggtattatta gattttacgt gtttaaaata aaacgaagaa
     6301 ggcggcgcgt aaacgcgccg ccttatcaat taccatcgaa ataagtattt cctattaata
     6361 gatattatct attgaaattt ctttcaggtc tttcttcttt tggtcttgca acagaaacga
     6421 caagctttct gccttctaac tctttctcgt tcatttctga gattgctctt tcagcttctg
     6481 aatcgtcttc taactcaacg aatccgaaac ctttgcttct tccgctcatt ctatcaaaga
     6541 tgatagatgc agatagaaca ttcccgtatg atgagaataa gtctcttaaa gcgtcatctt
     6601 taacgctgta aggtaagtta cctacgtata atttggtcaa actaacacac caccttccaa
     6661 aaacaaaata caatttaagt ctctctagaa atgggaaata ctttataagt ttgaggaccg
     6721 caagattccg aacattcgta agagaacaaa aggattatag cattctttta aataattaaa
     6781 tagtatcttt ttcgt
//
LOCUS       DAOTFA010000021         6447 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate AMR_MDS_3115
            NODE_8587_length_6447_cov_6.551314, whole genome shotgun sequence.
ACCESSION   DAOTFA010000021 DAOTFA010000000
VERSION     DAOTFA010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868139
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (wastewater metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6447)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6447)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:36:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 536
            CDSs (total)                      :: 521
            Genes (coding)                    :: 520
            CDSs (with protein)               :: 520
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6447
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8587_length_6447_cov_6.551314"
                     /isolate="AMR_MDS_3115"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1321
                     /locus_tag="P5014_01295"
     CDS             <1..>1321
                     /locus_tag="P5014_01295"
                     /inference="COORDINATES: protein motif:HMM:NF012652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GspE/PulE family protein"
                     /protein_id="HRY56777.1"
                     /translation="DEQKGKELGEEISAAMEEITQNTVKIEEGQAEMTDATIKDAPIA
                     RVVGMILETSVKLGASDVHIEPGEDYCRLRYRVDGILSEKRKIPKEMTDSVVARIKIL
                     AGMKIDEKRIPQDGRFKVQVGKTETDLRVSTLPTVFGEKVVIRLLKEEGTVFTYRDLG
                     LRGLALKRFEEMSLKPNGMILVTGPTSSGKTVTLASTLNKLNTIRVNVVTIEDPVEIR
                     VPGVNQVQVNPQAGLTFASALRAFLRQDPNIIMVGEIRDGETAELAVHAALTGHLVLS
                     TLHTNSSAGAVPRMLDMGIEDFFLSSTILGVLAQRLVRKVCPYCKEEYEPPEEIADDL
                     NKVLKELSSNTVLMTKDTELAKIIKSVMAKDKLTLYRGKGCPKCDNGYKGRVGIFELM
                     TMSEKIGEATLSKAPSTKIEQIAKEEGMITLLQDGYVRALQGETTLE"
     assembly_gap    1322..1421
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1422..2240
                     /locus_tag="P5014_01300"
     CDS             <1422..2240
                     /locus_tag="P5014_01300"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilT/PilU family type 4a pilus ATPase"
                     /protein_id="HRY56778.1"
                     /translation="RFRVNAFFQKGYPSMSLRYIKMQIPTFDELHLPPIINSVCNLKQ
                     GLVIVVGPTGQGKSTTIASMIDHINNTRAEHIVTIEDPIEYVFNNKLSLIEQREMFLD
                     SHSWDISLRSVLRQDPNVVFIGEMRDSDTIRSALQIAETGHLVFTTLHTNSASQTVDR
                     IISSFDVEKQNEVKSQLSQVIEVIISQRLLPSAKQGTIVACELMVKNDAISNLIREGK
                     AHMIDNVINTSAQYGMISMEKSIASLVTAGLVTVEDGLKYSAKPGELRRLIDKK"
     gene            2243..3445
                     /locus_tag="P5014_01305"
     CDS             2243..3445
                     /locus_tag="P5014_01305"
                     /inference="COORDINATES: protein motif:HMM:NF012694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HRY56779.1"
                     /translation="MSMFSYTAKNNIGGTVSGDVEARNREIAVSLLKNQGFTVISIKE
                     KSSDVSDFFSDLRGVPFGEVVTFTRQLSTMISAGLPISRSLEVLSNQAENKKFKKIIT
                     EILRSVEGGSSLSASFSTYPKVFSNTYISLVRAGESSGKLDIILRKLADNMEADRELG
                     SKFKSAMIYPMVIMIAMVAVVIILMIFVIPKLTEMYEGMQIELPAITKFVIGLSNFVI
                     KYIYIMLILVIGAVIGIRSFLSSEKGKTIISEIVIKIPVFGKITLQKDYASFSRTLSL
                     LIGAAVPIVDALNIVSVTMSGMQLRRSIQEAARQVEKGNSLSAFFKSDNTFPSLLGQM
                     TGVGEETGKTDEVLERVAVYYEGEVNNLVKGLSSALEPIILVMLGTMVGFLVVSVITP
                     IYKLTSSI"
     gene            3511..4020
                     /locus_tag="P5014_01310"
     CDS             3511..4020
                     /locus_tag="P5014_01310"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRY56780.1"
                     /translation="MRKQKGFTLVEMLVVIAIIAILAGAVLLAINPVATMRKSRDTTR
                     LSDLDSLRSAINIALTEGEINLVDMAAPETSANNIQAVDGTTGYVRFTIPGEGTPQAK
                     TGLGRYIPALPLDPTNAGTLVYSFQSTADNYEINAVLEYVDNEVKMTTDGGDNDAVYE
                     VGTALNIIN"
     gene            complement(4187..4639)
                     /locus_tag="P5014_01315"
     CDS             complement(4187..4639)
                     /locus_tag="P5014_01315"
                     /inference="COORDINATES: protein motif:HMM:NF024061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNHCP domain-containing protein"
                     /protein_id="HRY56781.1"
                     /translation="MNIKEEEKNKGFICINCKKWVPVSKYDQTVNRNHCPHCLWSKHV
                     DSDVTGDRMSFCNTGMKPIGLTSKTPRIDKWGMTVKGEIMLIHECIKCGKISINRIHA
                     QDNVGEIMNVFENGILLSPEKNNSLKQREIVILEENNREEVKKSILGE"
     gene            4785..5672
                     /locus_tag="P5014_01320"
     CDS             4785..5672
                     /locus_tag="P5014_01320"
                     /inference="COORDINATES: protein motif:HMM:NF013632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HRY56782.1"
                     /translation="MQAVIFIYLFIFGAFTGSFLNLISDRLPAGRKVIFGRSICDFCK
                     KKLKVKNLIPIFSFLFQKGRCSFCQKKLSFYYPLSEFLTGVAFMLAGHLSRFSYEINP
                     DNTFLLFYYIVVFSFFMVMILTDMKFCLILDSVIIPAIIFVFLSSLLFKFLDLSEIYT
                     NLKNDSFGVYLLRTDFFTSRVIMSLKNFGYVVASAILIALFFQFLIFITKGRGMGDAD
                     VKLGFLIGLVNGFPMAFMAVFMGYVLGAVYSVFLMLMKKKTMKDAIPFGPFLILGSVL
                     ILLFGNQIWVWYSGLGSIF"
     gene            complement(5706..>6447)
                     /locus_tag="P5014_01325"
     CDS             complement(5706..>6447)
                     /locus_tag="P5014_01325"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRY56783.1"
                     /translation="VASHELRTPMTIVKSYLWMLEQQKVGRLKQKQMEYLQKASRGTQ
                     RMIDLINDMLNISKFEQDKVFFNIQQVDLCDLIKEIVTSFEIKVNGKKIYLKFDESCS
                     DAFVDIDKEKMRDVLMNLIDNAVKFTNQGGIEIGIQIDLDKVTLSIKDSGSGISQEDL
                     PKLFHKFGRIDNSYTIASDTGGTGLGLYIAKLYLEGMGGKIWATSEGTNKGSTFWISI
                     PKSKINTRSPDFNSRVGKNFSNSVSSIR"
BASE COUNT         1918 a         1044 c         1182 g         2203 t
ORIGIN      
        1 tggatgagca gaaaggaaaa gaactaggcg aggaaatttc cgccgcaatg gaagagatta
       61 ctcagaatac tgtaaagatt gaagaaggtc aggctgaaat gaccgacgcc actatcaaag
      121 atgccccgat agcaagagta gttggtatga tactcgagac ctcggtgaag ttgggagctt
      181 cggatgttca cattgagccc ggtgaggatt attgcagatt gagatataga gttgatggta
      241 ttttgtcaga gaaaagaaaa attccaaagg aaatgacaga ttcggttgta gcaagaatta
      301 aaattcttgc tggaatgaag attgatgaaa aaagaattcc acaagatggt aggtttaaag
      361 tccaggttgg aaaaactgaa actgacttga gagtttcaac ccttcctaca gtttttggcg
      421 agaaggttgt tattagactt ttaaaagagg aaggaactgt ttttacatat agagatttag
      481 gtttacgtgg attggctctt aaaagatttg aagaaatgtc tttaaaacct aatgggatga
      541 ttttagttac tggacctact tcttcaggta aaactgttac tttagcttcc actctaaata
      601 aacttaatac aattagagta aatgtggtta ctatcgaaga tcctgttgaa attagagttc
      661 ccggagttaa tcaagttcag gttaaccctc aggcggggtt aacatttgca tcagcacttc
      721 gtgctttttt aagacaagac cctaacatca tcatggtagg agaaatcaga gatggtgaga
      781 cagccgaact tgcagttcac gccgctttaa caggacactt agttttatct actctacata
      841 ccaattcttc agctggtgct gtaccaagaa tgttggatat gggaattgag gactttttct
      901 tatcctctac tatcttaggg gtgcttgctc aaagattggt cagaaaagta tgcccttatt
      961 gtaaagagga gtacgaaccg ccggaggaaa ttgctgacga tttaaataag gttttgaaag
     1021 aattgtcatc aaatactgtt ttaatgacta aagataccga gttggctaaa ataattaaaa
     1081 gtgtaatggc taaagataaa ttaactttat accgtgggaa aggttgccca aagtgtgaca
     1141 acggttacaa aggcagggtt ggtattttcg agcttatgac tatgtccgag aaaatcggtg
     1201 aggctacact ttcaaaagct ccctctacaa aaattgaaca aattgcaaaa gaagaaggca
     1261 tgatcaccct cttacaagat ggttatgtta gagctctcca gggagaaact actttagagg
     1321 annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nagatttaga gttaatgctt
     1441 tttttcaaaa aggctatccg tccatgtcgc taagatatat aaagatgcag atcccgactt
     1501 ttgatgaact gcatcttccc ccaatcatca acagtgtttg taatttaaaa caaggtttag
     1561 ttattgttgt agggcctaca ggacagggta aatcgaccac aattgcttct atgattgatc
     1621 atataaacaa cactagagct gagcacatcg ttaccattga agaccctatc gaatatgttt
     1681 ttaataataa attgtcgtta attgaacaaa gagagatgtt tttagactca cattcttggg
     1741 atatctcatt aagatcggtt ttaagacaag atcctaatgt agtttttatt ggtgagatga
     1801 gagacagtga caccattaga tcagctcttc agattgcgga gacagggcat ttggttttta
     1861 ctactttaca tacgaactca gcttctcaaa cagttgatag aataatttca tcttttgatg
     1921 tagaaaaaca aaacgaagtt aaatctcagt tatctcaggt tatcgaggtt ataatatctc
     1981 agagattgct tccttctgct aaacaaggca ctatagtagc ctgtgaatta atggttaaaa
     2041 atgatgctat ttcaaatctt ataagagaag gtaaagctca catgatagat aacgttatta
     2101 ataccagcgc tcaatatgga atgataagta tggaaaaaag catagcaagt ttagtaactg
     2161 ctggtttagt tactgtagag gatggtttaa aatattccgc taaaccaggt gagttaagga
     2221 gactcatcga taaaaaataa atatgtcgat gttttcctac accgctaaaa ataacatcgg
     2281 gggaacggtt tcaggtgatg tcgaagccag aaacagggaa attgccgtaa gtttattaaa
     2341 gaatcaaggt tttactgtta tttctataaa agaaaaatct tcagacgtaa gtgatttctt
     2401 ttctgatctt agaggtgtac cttttggaga agtcgtcaca tttaccagac agctttctac
     2461 tatgatttct gcaggacttc ctatttcaag gtccttggaa gttctatcta atcaggctga
     2521 aaataaaaaa tttaaaaaga ttattactga gattttaaga tcggttgaag gcggatcttc
     2581 tctttctgct tctttttcta cttacccaaa agttttttcc aatacatata tatctttggt
     2641 tagggctggt gagtcgtcag gtaagttgga tattatttta cgaaaacttg ctgataatat
     2701 ggaggcagac agagaattag gttcaaaatt caagtcagcc atgatatacc caatggttat
     2761 catgattgca atggttgctg tggtaattat cttaatgata tttgttattc caaaattaac
     2821 cgaaatgtac gaaggtatgc aaatagaact tcctgctatt acgaaatttg taattggact
     2881 atctaatttt gttattaaat acatctatat aatgctgata cttgtcatag gtgctgtaat
     2941 aggaatcaga tcttttctat cttcagaaaa gggtaagact attatttctg aaattgttat
     3001 taaaatacct gtttttggaa aaatcactct tcaaaaagat tacgcaagtt ttagtagaac
     3061 gctttcactt ttaattggag ccgccgtccc gatagtggac gctttgaata ttgtttcggt
     3121 tactatgtcg gggatgcagt taagaaggtc tattcaggag gcagcaaggc aggttgaaaa
     3181 aggaaactca ttgtcagctt tttttaagag cgataatact tttccttcgt tgttgggtca
     3241 gatgaccggc gttggtgaag agacaggaaa gacagatgaa gttttggaaa gagttgctgt
     3301 ttattatgaa ggtgaggtta ataatttggt taaaggtttg tcctctgcat tggagccgat
     3361 aatccttgtt atgttaggaa cgatggtagg gtttttagtt gtatcagtta ttactcctat
     3421 atataagttg acaagttcta tttaatttaa tgtacaatta attagtttaa aaattataag
     3481 aaacacacaa cgagaggggg tgaattaatt atgagaaaac aaaaaggttt tacattagtg
     3541 gaaatgttag ttgttattgc aattattgca attctagcag gtgctgtttt gttggcaatt
     3601 aatcctgtag caacgatgag aaaatcaaga gacacaacca gacttagcga tttagattct
     3661 ttgaggagtg ctatcaacat tgcattgacg gagggtgaaa ttaaccttgt agatatggcg
     3721 gctccagaga caagcgcaaa taatattcag gcggttgatg gaacaactgg ctatgtgagg
     3781 tttacaatcc ccggtgaagg tacaccccag gcaaagactg gtttaggacg atatatcccc
     3841 gcccttccgt tggatcctac aaatgctggt acgttggtct actctttcca atctactgca
     3901 gataattatg aaattaatgc agtattggaa tatgtggata acgaagtcaa aatgacaacg
     3961 gatggtggtg ataatgatgc tgtttacgaa gttggtacag ctttaaatat aatcaattaa
     4021 cattggttat tcagagatga tctttgaaag cagatgcccc cgccttcggc gggggcatcc
     4081 ttttgtatac ttttttattt gctctcgtgt acttttagac ttcttttcag ttacttaaca
     4141 ccttcaatac cctttactga tatattttct ctaccttata tataaactac tcacccaaaa
     4201 tacttttttt aacctcttcc ctattatttt cctcaagaat aactatctct ctttgtttca
     4261 aagaattatt tttttcagga cttaataaaa ttccattttc aaacacattc ataatttcac
     4321 ccacattatc ttgagcatgt attctattta tggatatctt tccgcattta atacattcgt
     4381 gaataagcat tatttcacct tttacagtca ttccccattt atcaatcctt ggtgtttttg
     4441 atgttaatcc gattggtttc atcccagtgt tacaaaatga cattctatct ccagttacat
     4501 cgctatctac atgttttgac caaaggcagt gagggcaatg atttctattt acggtctgat
     4561 cgtacttact gactggtacc cattttttac aatttataca aataaaacct ttgttttttt
     4621 cttcttcttt tatattcata agttaaatta tatctaaaaa gcttgcctct ttcttaatgt
     4681 gcgttttcct ttagttctga ttccaaaaat atttcactca tccctactcc tctaaaaaaa
     4741 tcatacactt tttaaaatac ccttgttact gtaatataca ttttatgcag gcagtaattt
     4801 ttatatactt gtttattttt ggagccttta caggttcttt tttaaatctt atatccgacc
     4861 gccttcccgc cggcaggaaa gttatttttg gacgttccat ctgcgatttc tgtaaaaaga
     4921 aattaaaagt aaaaaacctc atcccaattt tttctttttt atttcaaaag ggccgatgta
     4981 gtttttgcca gaaaaaactt tctttttact acccgctttc cgaattttta acaggtgtgg
     5041 catttatgtt agctgggcat ctctcaagat tctcgtatga aataaatcct gacaacacat
     5101 tcttactttt ttactatatc gtagtttttt catttttcat ggtgatgatt ttgaccgata
     5161 tgaaattttg tttaattctc gattccgtaa taattcctgc gatcatcttt gtttttcttt
     5221 cttcattatt atttaaattt ttagacttat cggagattta tactaattta aaaaatgata
     5281 gttttggtgt ttatttattg agaacagatt tttttaccag tagggttatt atgtctctga
     5341 aaaattttgg gtatgttgtg gcaagtgcaa ttctaattgc actttttttt caatttttaa
     5401 tttttattac caaaggtagg ggtatgggag atgcggatgt taaactcgga tttttaatcg
     5461 ggttagttaa tggttttcct atggctttta tggctgtttt tatggggtat gttttaggcg
     5521 cagtttacag cgttttttta atgctgatga aaaagaaaac tatgaaggat gccatacctt
     5581 tcggtccgtt tttgatatta ggaagtgtct taatactttt atttggaaat cagatttggg
     5641 tatggtacag cggtttaggg agtatttttt aaaaattacc ttcgagatag tattttgatc
     5701 cctctttatc ttattgaaga aacactgttt gaaaaattct taccgactcg tgaattgaaa
     5761 tcaggcgacc ttgtgtttat tttacttttg gggattgata tccaaaaagt actaccttta
     5821 tttgttcctt cggatgtagc ccatatcttg ccccccattc cctctaaata tagttttgct
     5881 atatacaatc caagacctgt tccccctgta tccgaggcta tagtgtagga attatctatc
     5941 cttccaaatt tatggaaaag ctttggtaaa tcttcctgac taattccgga cccgctatcc
     6001 tttattgata acgttacttt atcaagatca atctgtattc ctatttctat tcctccttga
     6061 ttagtaaatt taaccgcatt atcaatcaaa ttcattaata catctctcat tttttcttta
     6121 tctatgtcga caaaagcatc tgaacatgat tcatcgaatt ttagatagat ttttttaccg
     6181 ttgaccttta tttcaaagct agtaactatt tcttttatta gatcgcataa atcaacttgt
     6241 tgaatattaa aaaatacctt atcctgctca aattttgata tgttcaacat atcgtttatg
     6301 aggtctatca tcctttgagt tcctctcgaa gctttttgta gatactccat ttgtttttgt
     6361 tttagcctcc caactttttg ttgctcaagc atccatagat aacttttaac tatagtcata
     6421 ggagttctta actcgtgtga ggcaaca
//
LOCUS       DAOTFA010000022         6425 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate AMR_MDS_3115
            NODE_8626_length_6425_cov_4.679749, whole genome shotgun sequence.
ACCESSION   DAOTFA010000022 DAOTFA010000000
VERSION     DAOTFA010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868139
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (wastewater metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:36:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 536
            CDSs (total)                      :: 521
            Genes (coding)                    :: 520
            CDSs (with protein)               :: 520
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6425
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8626_length_6425_cov_4.679749"
                     /isolate="AMR_MDS_3115"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(52..600)
                     /locus_tag="P5014_01330"
     CDS             complement(52..600)
                     /locus_tag="P5014_01330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56784.1"
                     /translation="MNFLAIFLSKYSAYIMVIILSSIGLVFFLSVMKKDNLKNDSKEV
                     TIYFLINQLSTVAIYLIAKALLSVFHIYPGEDTDIILFIFLNFLCIYIETVAFYKLNE
                     RVSYSSKNKDAGSRDYQKEIFSSVRDFCLLLVLILVPLYVFGNGSLNSFILFAFLDIL
                     VVFFTHTVLLPLVLKKYSKSSK"
     gene            complement(597..1952)
                     /locus_tag="P5014_01335"
     CDS             complement(597..1952)
                     /locus_tag="P5014_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56785.1"
                     /translation="MSGKQTFKLVLIALIFSISVLIVLPEIPVVVDSDYLILNSSIGG
                     MQVKIPWSKDGKVLDLKEFKKGSGIGESKKLSFELKNKDLENNPEVISRLLSVTQKRL
                     NLSRISDYKVSQESEDKISITIPTFEDSDRINSIVTGSGKIIFRTVKNPQDWSKDKFQ
                     NFYLESDRWQDTDINENDVQGFVNVMDQTGVYKMQIVFTDIGRQKFYDLAGKNIGLPI
                     AVYVSNFDYPFLMPMIGENILDNKNLDPAVNNTYPQYIIDDYNLQIKNPLPEGISAVE
                     TTQVPPVMGSNFIEKYAKAFFISILLISAYFLLKFNVRGLVFSYSLLLSVLIYLAISK
                     LFSIPVGTQMFLSLILVMGIMTYIGDAIHTKINKDLISGVPFEISFNQVLVKDKSAIS
                     YPSILILSVSLILYFISSGFSRTFYFSTFAGMLVVVLFYSFLFPTLLWAFKGGERPKE
                     K"
     gene            complement(1971..2552)
                     /locus_tag="P5014_01340"
     CDS             complement(1971..2552)
                     /locus_tag="P5014_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56786.1"
                     /translation="MLNKILVSEVRVNILKLLLLNPNRAFHIRAIVRQVGAEINAVRR
                     ELDNLFEINLVNRRQSSNKIFYQIETSHPFFSDLLSLVAKEEGVGAAIVKNEKRIGMI
                     DYAVLSLAFLRGRKSTALDVDLFIVGDVDLEVIKNVVKEEEGKMNKEINYSVMNMEEF
                     MSRKRTNDPFIYRVMTQSRTMLIGDDEKFCAVV"
     gene            complement(2634..3908)
                     /locus_tag="P5014_01345"
     CDS             complement(2634..3908)
                     /locus_tag="P5014_01345"
                     /inference="COORDINATES: protein motif:HMM:NF022702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HRY56787.1"
                     /translation="MPKITNITPGFDIDDLSAGNIEEVSKTKSEFEKNIINKDSSSKN
                     SKTKARYFTSKNSNHKLKKILLILLAVISILFSLWIVLVKTGKIGKKELNPLSDKNLM
                     DNVVKVTADLTGVQYPETEAGSWVNERPFGVMINNHIDARPQSGLVYADLVYEIVAEG
                     GITRFLAFFHTITPEKIGPVRSTREYYLVLVKELSDAMIMHIGYSPQALLAIDTWPVR
                     SLFRGGCESYPGCTWRDNPRNVAYEHTAYVNGVKLRELGLKLGWEGKGDIRMYTFKDN
                     SEKYSTSSSASKISIDFWNKGDYSSVFEYNPQNNTYLKFTGYDSADNPIPHKDQETGE
                     QVEVKNLIIQFATENSIVGDDKGRLDYQLVGSGKALVFIDGKVVDATWSKASRDERTL
                     FYDTNGQEIEFNRGKFWIAIVPDRNVEQVVYN"
     gene            complement(3966..4583)
                     /locus_tag="P5014_01350"
     CDS             complement(3966..4583)
                     /locus_tag="P5014_01350"
                     /inference="COORDINATES: protein motif:HMM:NF016119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0016485 - protein processing [Evidence
                     IEA];
                     GO_process: GO:0045184 - establishment of protein
                     localization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HRY56788.1"
                     /translation="MTRLGLLLVALAFLGGLVIFFPVIKKEISFRLNKPNENTPVASS
                     ELDSMRFFGQDDVIIAKDPLFSIIIPKIAANSKVIADVDPNDSKVYQVALTKGVAHAK
                     GTSYPGEFGNSFYFAHSSDNFYNANRYNSVFYLLNKMETGDFFYLVYENWVYKYIVTE
                     VSVVKPDDIKYMENDTNKKTATLMTCWPAGTTISRLVVVGNLAEK"
     gene            complement(4606..>6425)
                     /locus_tag="P5014_01355"
     CDS             complement(4606..>6425)
                     /locus_tag="P5014_01355"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56789.1"
                     /translation="VQWNWSQSYGGGCPVMGVTDIYCSNGYGIVQRIADLNLAQDVYH
                     DEVYNLRVNPGDVRSCYIGNVENSELRISETNDSWPNTLEIGDTFTYTIEVRAVGGPV
                     NDVVMINLPPQGFEPVPITFSAVSTDAGHVIDTSGVVYASPGTWDLGNLDEDEVVTIT
                     YEAKVTEDVDSGIYPDVSFAQGEGMGGTGLLALSEESGFPINDGVVDENFVGTQVKIA
                     VELELNDEVDVKEEEEGEVLGASTELPATGASTFWVNLVLVLGSIGGLLLLIGGLGTM
                     KKAKKLFMILFAIAGLSYVLNVKANAFSLPQPVVRISEPKSPVGENFNVTFVVLSTDS
                     GQSMTAKCMAINPGSSTPYQFGSDINVTPGGSSYNCEVGESVLDDSGTYKFYVLVDPD
                     SADDVKSDTVEVYYDGIGPDKPKYIEKEKVNDCVNKITLKTADDGETSSVRVYADDDK
                     EIDIDDSHKIETESIGPNEKFDFEHIVSGDSCDKTWYYAVVAFDDAGNASKPRSEVIT
                     TTTTTTEEEEEETGAIPVEGGTGLTEGEVAGEGATGPEGTEDEGLEVTLGEGEEGSVL
                     GEETGGEESGKGLFKSPWFWIAAAGLGIVIISATKKKKA"
BASE COUNT         1980 a         1317 c         1002 g         2126 t
ORIGIN      
        1 aatttcccat ttatacatat aatatatttc caaaactaag gaaacggata tttatttgga
       61 ggattttgaa tactttttaa gcaccaatgg taaaagtacc gtgtgagtaa aaaacactac
      121 caaaatatcc aaaaacgcaa ataaaataaa actatttaaa ctgccgtttc cgaaaacata
      181 tagcgggact aagattaaaa ctaacaacaa acaaaaatct ctaaccgatg agaatatctc
      241 tttttgataa tctctcgagc cggcatcttt atttttactt gaataactta ccctttcatt
      301 taatttataa aatgctaccg tctcaatgta gatgcataag aaattcagga atataaacaa
      361 gataatgtct gtatcttctc cgggataaat atgaaaaaca cttaaaagtg ccttggcgat
      421 taaataaata gcaacagtgg atagctgatt tattaaaaag tagattgtaa cttctttcga
      481 atcgttcttg agattatctt ttttcattac cgacaagaaa aataccagac ctattgaaga
      541 aaggattatt accattatat atgctgaata tttagataaa aatattgcta agaaattcat
      601 ttttctttag gtctttcacc acctttaaat gcccacaaga gagtgggaaa taaaaatgaa
      661 taaaataata ctactaccag catcccggca aaggttgaaa aatagaaggt tctggaaaac
      721 cccgatgaaa tgaaatataa aatcagagat acagataaaa taagaattga gggatatgaa
      781 attgcacttt tatctttaac taaaacttga ttaaaagata tttcaaaagg aaccccagag
      841 attaaatctt tattaatttt tgtatgaatc gcgtctccga tataagtcat aattcccata
      901 acgagaatta gggataggaa catttgggta cctactggta ttgagaataa ttttgaaatt
      961 gctaggtaaa ttaaaacaga gagcagtaga gagtaagaaa acaccaatcc tctaacatta
     1021 aattttaata gaaaataagc agagatgagc agaatactta taaaaaaggc cttcgcatat
     1081 ttttcaataa aatttgaacc catcacagga ggaacttgcg ttgtttcaac tgcagatata
     1141 ccttcgggta aaggattttt tatttgtagg ttataatcgt cgataatgta ttgagggtaa
     1201 gtattattaa ccgccgggtc caagttcttg ttatcaagga tattttcacc aatcatgggc
     1261 attagaaaag gatagtcaaa attactcacg taaacagcta ttggtaatcc aatattttta
     1321 ccagccaaat cataaaactt ctgtcttccg atatctgtaa aaactatttg cattttataa
     1381 acacccgtct gatccattac attaacaaaa ccttgaacat cgttttcatt tatgtctgta
     1441 tcttgccacc tgtcagattc taaataaaaa ttctgaaatt tatctttact ccagtcttga
     1501 ggatttttaa ctgttctaaa aattattttt ccgcttccgg tcactatact gttaatcctg
     1561 tcgctgtctt caaaagtagg aatagtaatt gaaatcttat cctcactttc ttgacttact
     1621 ttataatcag atatcctgga gagatttagc cttttttgag taacagaaag caaccttgag
     1681 ataacttctg gattattttc taaatctttg ttttttaact cgaatgataa ttttttactt
     1741 tcaccaatac ccgatccttt tttaaactct ttcaaatcaa gcaccttgcc atcttttgac
     1801 cacggaattt ttacctgcat gcctccgata cttgagttaa gaataaggta atctgaatcg
     1861 acaacaaccg ggatttcagg caaaacaata agaacagaaa ttgaaaaaat taacgctatt
     1921 aatactaatt tgaaagtttg tttccccgac atgtttaaac attagaaatt ttacactact
     1981 gcacaaaatt tctcatcatc tccaattaac atagttctac tttgagtcat tactctgtaa
     2041 ataaaggggt cgttagttct ttttctgctc ataaattctt ccatattcat aaccgaatag
     2101 ttaatttctt tattcatctt gccttcctct tctttcacga catttttaat aacttctaaa
     2161 tcgacatctc ccactataaa aagatccaca tccaaagccg tagattttct ccctcttaag
     2221 aaagccaaag ataaaaccgc gtaatctatc attccaattc ttttttcatt tttaactata
     2281 gccgcaccta ctccctcttc cttagccacc aaagacaata aatccgaaaa aaacggatgg
     2341 cttgtctcaa tttgataaaa aatcttattt gaactttgtc ttctatttac gagattaatt
     2401 tcaaaaaggt tgtccagctc tcttctgact gcgtttattt ctgctccaac ctgtctcaca
     2461 atagctctaa tgtgaaatgc cctgttagga tttaaaagaa gaagtttaag tatgttcact
     2521 ctgacttctg ataccaatat tttattcagc atattttaat tccgggcaga ttatttataa
     2581 acctataact tagatacatt atacattttt acgaccgttt tcacaggtat tttttagtta
     2641 tagaccacct gctctacatt tctatcggga acaatcgcta tccaaaactt acctctgtta
     2701 aattcaattt cctgaccgtt agtatcataa aataaggttc tttcatctct ggaagccttg
     2761 ctccaagtag catctacaac cttaccgtca ataaaaacaa gggctttacc gctaccaacc
     2821 aactgataat caagccttcc tttgtcatct ccgactattg agttttcagt ggcgaattga
     2881 ataattaggt tttttacttc aacttgctca ccggtttcct gatctttatg aggaattggg
     2941 ttgtcggcag aatcgtagcc tgtaaatttt aggtaagtat tgttttgagg attgtattca
     3001 aaaacactgg aataatcacc tttattccag aaatctattg aaattttgga agctgatgaa
     3061 ctcgtagaat atttttcaga attatcctta aatgtataca ttcttatatc tcccttacct
     3121 tcccacccca gcttcaaacc taattctctt agttttactc cgtttacata agcagtgtgc
     3181 tcgtaagcca catttcttgg attatctctc caggtacatc cgggataaga ttcacaacca
     3241 cctctgaaaa gacttctaac cggccacgta tctatggcca gtaaagcctg gggtgaatac
     3301 ccaatatgca taatcatagc gtcgcttaat tcctttacta aaactaaata atattctctg
     3361 gtgctcctga cagggccgat tttttcaggt gttatagtat ggaaaaacgc caaaaacctt
     3421 gttataccac cttctgcaac tatctcataa actaaatcag cgtacacaag cccggactga
     3481 ggtcttgcat caatatggtt atttatcatt acaccaaaag gtctttcatt tacccaagaa
     3541 cctgcctctg tttccggata ctgaacacct gttagatcag ctgttacttt aacaacgttg
     3601 tccataaggt ttttgtcgga taaagggtta agttcttttt tgcctatctt tcctgtttta
     3661 actaacacaa tccacaatga aaaaagtatt gaaataactg ccaaaagaat tagaagtatt
     3721 tttttgagtt tatggtttga gttttttgaa gtgaaatatc gagctttagt tttggaattt
     3781 tttgatgatg aatctttgtt tattatgttt ttttcaaact cggattttgt tttagagact
     3841 tcttctatat ttccggcaga aaggtcatct atatcaaagc cgggggttat gttagttatt
     3901 ttaggcatat agaaatttta caggattgta caatttttta ctaccgttta aattttattg
     3961 aaaaattatt tttcagctaa attacctacg accactaacc tgctaattgt agttccggcc
     4021 ggccaacagg tcataagagt tgctgttttt ttattggtat cgttttccat atatttaatg
     4081 tcatcaggtt ttaccacaga gacctcagta acaatgtatt tataaaccca gttttcataa
     4141 acaaggtaaa aaaagtcccc tgtttccatt ttgtttaaaa gataaaaaac agagttgtat
     4201 ctatttgcgt tataaaaatt gtcggatgag tgtgcgaaat aaaaggaatt tccgaattct
     4261 cccggataag acgtgccctt agcgtgagcc acacctttgg ttagtgctac ttggtaaact
     4321 tttgagtcat ttggatctac atccgcaatt acctttgaat tagcagcaat tttagggatt
     4381 attatgctaa aaagaggatc tttagcgata atcacatcat cctggccaaa aaacctcata
     4441 gaatcaagct cagagcttgc tacgggggta ttttcattgg gtttattaag cctaaaagag
     4501 atttctttct tgattacagg gaaaaagata actaacccac cgaggaaggc tagggctact
     4561 aaaagtagcc ctagccgtgt cattttcttg tatatgtttt ggcttttaag cttttttctt
     4621 ctttgtcgca ctgatgatta ctatacctaa acctgctgcc gcaatccaga accaaggtga
     4681 tttaaataat cctttaccgg actcttcacc acctgtttct tctcctaaaa cagaaccttc
     4741 ttcgccttca ccaagagtaa cttctaaccc ctcatcttcg gttccttcag gccctgttgc
     4801 gccttctcct gcaacctcac cttctgtaag gccggtacca ccttccaccg ggattgctcc
     4861 tgtttcttct tcctcttctt cggtagttgt tgtagttgta gttataactt cggaccttgg
     4921 tttggaagca tttcccgcat cgtcaaatgc cacaacagca tagtaccaag tcttatcgca
     4981 actatcaccg ctgactatgt gttcgaaatc aaatttttca tttggtccaa tggactcagt
     5041 ttctattttg tgcgagtcat ctatgtctat ttctttatcg tcatcggcat aaactctgac
     5101 tgaagaagtt tctccgtcat cagctgtctt taaggttatt ttatttacac agtcatttac
     5161 cttttctttt tcaatgtatt ttggtttatc tggaccgatg ccatcgtaat aaacttccac
     5221 tgtgtctgat ttcacatcat cagcactatc aggatcaact aagacataaa atttatatgt
     5281 accgctatca tcaaggaccg attcacccac ttcacagttg taagaacttc ctcccggagt
     5341 aacatttata tcggacccaa attggtaagg agtggacgaa cccgggttta ttgccataca
     5401 ttttgcagtc attgattggc ctgaatctgt actcaacact acaaaggtaa cattaaaatt
     5461 ttcacccaca ggtgattttg gctcactgat tctaacaacg ggttgaggaa gtgagaatgc
     5521 attagctttg acatttaaaa cataagataa acccgctata gcaaatagaa tcataaatag
     5581 ttttttggct tttttcattg ttcctaaacc tccgatgagc agtagtaaac cacctattga
     5641 acctagcact aacacaaggt tgacccagaa tgtactcgca cccgtggctg ggagttcggt
     5701 cgaagctcca agaacttcgc cttcttcctc ttcttttacg tctacttcat cgttcagttc
     5761 aagttccact gctattttta cttgtgttcc cacaaagttt tcatccacca ccccatcgtt
     5821 gatagggaaa cctgattctt cggatagagc aagaagtcca gtaccgccca ttccttctcc
     5881 ttgagcaaaa gaaacatcag ggtaaatacc ggaatcaaca tcttccgtaa cttttgcttc
     5941 gtaagttatg gttactacct cgtcttcatc caaattacct aaatcccaag ttcccggaga
     6001 agcataaaca actccggaag tgtcgattac atgccccgca tcagtagaaa ctgcgctaaa
     6061 cgtaatcggc acaggttcaa aaccctgagg aggtaagtta atcataacca catcatttac
     6121 agggccccct actgctctaa cttctatagt gtatgtgaat gtatctccga tttctaatgt
     6181 gtttggccaa ctatcattcg tttcgcttat acgtagttcc gaattttcaa cgttaccgat
     6241 gtagcaggat ctaacatcac cgggatttac tcttaggtta taaacttcgt catgataaac
     6301 atcctgagct aaattgagat cggctattcg ttgaactatg ccataaccgt tggaacagta
     6361 aatatccgtt actcccataa ccggacaacc tcccccatag gattggctcc aattccactg
     6421 cacat
//
LOCUS       DAOTFA010000023         6402 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate AMR_MDS_3115
            NODE_8665_length_6402_cov_8.457697, whole genome shotgun sequence.
ACCESSION   DAOTFA010000023 DAOTFA010000000
VERSION     DAOTFA010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868139
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (wastewater metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:36:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 536
            CDSs (total)                      :: 521
            Genes (coding)                    :: 520
            CDSs (with protein)               :: 520
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6402
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8665_length_6402_cov_8.457697"
                     /isolate="AMR_MDS_3115"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1946)
                     /locus_tag="P5014_01360"
     CDS             complement(<1..1946)
                     /locus_tag="P5014_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008908810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HRY56790.1"
                     /translation="MFKLVSPFKPTGDQPQAIEKLTENIQSGTKDQVLLGVTGSGKTF
                     VMANVIKNLNKPALIISHNKTLAAQLYQEFRDFFPENAVEYFVSYYDYYQPESYIPST
                     DTYIEKDSAINEKIDKLRLSATTSLMTRKDVIVVSSVSCIYNLGSPVEYGKVAIELRL
                     GMKIRKTDLLKRLGQTYYERSDYDFKRGTYRVVGEVIDVYPAYQDFGLRLEIFNDILV
                     KITHFDPLTGDSIKKDETPYEKYGTGSNTPKENVEKYVKERDLREDFAVIFPAKHYVT
                     TEETREQAFAQIESDLDKRLFELRKENKIVQAYRLEQRTRYDLEMMREIGYCKGIENY
                     SRYFDGRNPGDAPYSLMEYFPKDFITIIDESHMSVPQIRGMFNGDRSRKQTLIDYGFR
                     LPSSLDNRPLNFDEFMKRRGSTVYVSATPDSWELDQSKKTVVELLLRPTGITDPEITI
                     VPTKGQVNDLLLRIQKKVSEGQRVLVTTLTKRMAEDLSSFFAEKKVKVTYLHSDVDTL
                     ERTDILDDLRKGEYDVLVGINLLREGLDLPEVGLVAILDADKEGFLRSETSLIQTMGR
                     AARHVQGEVVMYADNITGSMKRAILEVERRRNIQIEYNKEHGISPKQIVKPLREKLID
                     EVIEEQISGKKEAWQEVDYKQLPP"
     gene            2046..2678
                     /gene="lexA"
                     /locus_tag="P5014_01365"
     CDS             2046..2678
                     /gene="lexA"
                     /locus_tag="P5014_01365"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HRY56791.1"
                     /translation="MSPVTIYKKQKQILDFISQYIQVNGYSPTLQEIADAMGLSSLAT
                     VHEHLQALEKKNIIKRYDGAVRGIEVVDNSVNASLSAIELPLIGYIAAGEPIEAIENE
                     ITTVMVSPDMVSKSKRCFVLQVKGSSMIDEGIMDGDYVVLKQQETAENGQIVVALIDE
                     GFATLKKFYKEKDDKIRLQPANDEMEPIIVEANQVKIQGVVTGIVRRYKN"
     gene            2768..4087
                     /locus_tag="P5014_01370"
     CDS             2768..4087
                     /locus_tag="P5014_01370"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HRY56792.1"
                     /translation="MTSLIAPIITYLEKLAQYVPLEVFTVVGTFLEEVIAPIPSPFVL
                     TTAGTITKAQNNPLSYILFIAVLASLSKTFGGWLLYKITDKLEDVFTSKIGKFVGITS
                     NDVERIGKYFNGGKKDWLVLFTLRSVPIFPSSPISITSGLIKIKMKTFLITTFLGTII
                     RNLCYLYLGYAGLAASEEVMRGLNKSETVGEIIVVSLIGFALLWGYKKRKQIFSNNEN
                     GSSENKIKQAKDLLNYKTVDELPKDESEEFSTIYIFRHGQTDDNANFIFSGNRDAKLT
                     EEGKKQAKILSEKIKGIKFDRLISSPQNRAVQTMEIAMSQNQSAKNMKIETDERIRER
                     SYGDLTGKSKMEIQLNNPEELLKIRRSYDFVPTNGESIEMVCKRVEEFCDDLVKEISG
                     KNLKVAISCHGNSIRGFRKYFEKLDEETTAKLETPLGQDYASYVISN"
     gene            complement(4108..5331)
                     /locus_tag="P5014_01375"
     CDS             complement(4108..5331)
                     /locus_tag="P5014_01375"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF012341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HRY56793.1"
                     /translation="MRKIENIRSQKILDSRGNWTLETKVTLDDGSVGVQPVPSGASVG
                     ENEAVYIDVEKAADFVSTTINDVLVGKDPSNQKSIDENLIKMDGTSNKSRLGANSILS
                     VSLACAKSAAVSAGLELYQYLSLMYSGKQKLNGDLKFPTPIFNIINGGEHAKNKLSFQ
                     EFMVIPALNTPYNKALQMGASIYKDLKKILLKDDFETGVGDEGGFEPRGLSARTALDF
                     LEQAASVQFDPGNDVFFGLDIAAGSFYENGKYCIDEENLELSSDEVIKYYQDLFMHYK
                     IIYLEDPMFERDEFGWTKFYKQNAGKYLVVADDLVVTNPKILERVAKEKMANAVIVKP
                     NQIGTLTETLDFIKLARKSKMDIVISHRSGDNVDSFISDLAVAVDADFMKSGAPARGE
                     RVAKYNRLLEIFYEF"
     gene            complement(5344..>6402)
                     /locus_tag="P5014_01380"
     CDS             complement(5344..>6402)
                     /locus_tag="P5014_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HRY56794.1"
                     /translation="ELGVPAVVGTLNATKMLSNGEIITVDGASGKIYEGDITKVVQET
                     KKSSVDLSKIKTATKIYVNLAEPELASEMASRNTDGIGLLRAEFIIANIGTHPKKFIK
                     EGKKKEFILKLAEGLEKFADAFNPRPVVYRFTDFKSNEYAGLKGGAEFETDEPNPMIG
                     YRGVSRYIKDLDVFEMELEAIKIVRNKKGLKNLWVMMPFVRTVEQLREVKKIVSASGL
                     RRSGSFKFWMMVEIPSNVILLDDFIDEGIDGVSIGTNDLTMLTLGVDRDNEKVADTYN
                     EMDPAVLKSLEKIVTTCRKRDITCSVCGQAPSVFPELVEMMVEWGMTSVSISPDVIEK
                     TREIVYEAEKKVLNKKKK"
BASE COUNT         2051 a         1266 c         1010 g         2075 t
ORIGIN      
        1 atcggtggta gctgtttgta atctacctcc tgccaagctt ctttctttcc actaatctgc
       61 tcttcgataa cctcatctat aagtttttct ctcaacggtt ttactatttg tttaggagag
      121 ataccgtgct ctttgttgta ttcaatttga atgtttcgtc tcctttcaac ttctaaaatt
      181 gctctcttca tactgccagt gatattatca gcatacatca caacctctcc ttgaacgtgc
      241 cttgccgccc tccccatggt ctgtattaaa gaagtttcag atcgtaaaaa gccctccttg
      301 tcagcatcta aaattgctac taaaccaact tcaggtaaat ccaaaccttc cctaagtaaa
      361 tttattccca ctaaaacatc gtactcacct tttctaagat catccaaaat atcggttctt
      421 tccaaagtat caacatcaga atgcaaataa gtcaccttta cttttttctc agcaaaaaat
      481 gaggataggt cctccgccat tctttttgtt aaagtcgtaa ctagaactcg ctgaccttcg
      541 gatacctttt tttgaattct taaaagtaaa tcattcacct gcccctttgt gggaacaata
      601 gtaatttccg ggtccgtgat acctgtaggt cttaacaaaa gttctactac agttttttta
      661 gattgatcaa gctcccagct gtcgggtgtt gcacttacat aaacggtgct ccctcttctt
      721 ttcataaatt catcaaaatt aagaggtcgg ttgtctaaag atgaagggag cctaaaacca
      781 taatctatta aagtttgttt tcttgatctg tctccgttga acatccctcg tatttgcgga
      841 acactcatgt gtgactcatc aataatggta ataaaatctt tcggaaagta ctccattaaa
      901 gaataaggtg cgtcaccagg atttctccca tcaaaatatc tggaatagtt ttcaattcct
      961 ttacaataac ctatctccct catcatctcc aagtcgtatc tggttcgctg ttcaagtctg
     1021 taagcttgaa ctattttgtt ctcctttctt aactcaaaga gacgtttatc taagtcgctc
     1081 tcaatttgtg caaatgcttg ttcccttgtt tcttctgtag tcacatagtg ttttgctgga
     1141 aaaataacgg caaaatcctc tcttaaatct ctttctttaa catatttttc tacgttttct
     1201 tttggagtat tagatccggt accgtacttc tcataaggag tttcatcttt tttaatagaa
     1261 tctcctgtca atggatcaaa atgtgtaatt ttaactaaaa tatcgttaaa aatttctaac
     1321 cttaacccga aatcctgata agcaggataa acatctatta cctcaccgac taccctataa
     1381 gtacctcttt taaaatcata atcagaacgc tcataatagg tttgacccaa tctttttaaa
     1441 agatctgttt tcctaatttt catcccaagc cttaattcaa tagcaacttt tccgtattca
     1501 acaggagaac ctaagttata aatacatgaa actgaagaaa caacgataac atctttcctg
     1561 gtcattaaag aagtggtcgc agaaagtcga agtttatcga ttttttcatt tattgcagaa
     1621 tccttttcta tgtaagtatc tgtggaagga atataacttt cgggctgata gtaatcatag
     1681 tagctaacaa aatattcaac tgcattttca ggaaaaaagt ctctaaattc ttgataaagc
     1741 tgggcggcta gggttttatt gtgggaaatt atcaaagcag gtttattcaa attctttata
     1801 acatttgcca ttacaaacgt cttcccggaa ccggttactc ccaacaatac ctgatcctta
     1861 gtaccagatt gaatattttc agttaatttt tctatcgcct ggggttggtc gcccgtaggt
     1921 ttaaacggag atacgagttt aaacatagaa tgataatatc ataaaaagca cttgacatct
     1981 tcggttattt ttcggtatta taaaaatacg aagaaaaacc gtagttattt tttaaaagga
     2041 ttaaaatgag tcccgtaact atttataaaa agcagaaaca aattttagat tttatctcac
     2101 agtatattca agttaatggc tattccccca ctctccagga aattgcggat gccatgggat
     2161 tgtcatcgtt agctacggtt cacgaacatc ttcaagcttt agaaaagaaa aatattataa
     2221 aaagatatga tggagcagtg agaggcatag aggtagtaga taactcggta aatgccagtc
     2281 tctcggctat agaacttcct ttaattggtt atattgcagc aggagagcct atagaagcta
     2341 ttgaaaacga aattaccaca gtaatggttt cccccgatat ggtttcaaaa agcaagagat
     2401 gttttgttct tcaggtaaaa ggatcatcta tgatagacga aggtattatg gatggggatt
     2461 acgtagtttt aaaacaacaa gaaacagctg aaaacggtca aattgtagtt gcgttgatag
     2521 acgaaggttt tgctacccta aagaaattct acaaagaaaa agacgataaa ataaggcttc
     2581 agccggcaaa tgatgaaatg gagccaatta ttgttgaagc aaatcaggtt aaaattcagg
     2641 gagttgtaac aggaatagtt agaagatata aaaattaaaa ttattgagtc ttgggaaaaa
     2701 acaccctctt taaaattctt aaataatttg tgtagatacc ctctgtgatg tagaatttat
     2761 gggttgtatg accagcttaa ttgcacccat aattacatat ttagaaaaat tagctcaata
     2821 cgttccactt gaggtgttta ctgttgtcgg tacttttcta gaagaagtaa ttgcccccat
     2881 cccatctcct ttcgttctta caacagcggg aaccattaca aaagctcaaa ataaccctct
     2941 atcgtatatt cttttcatag ccgttcttgc ctcattaagc aaaacttttg gaggttggct
     3001 tttgtataaa ataactgaca agctcgagga tgtattcaca tcaaaaatag gtaaatttgt
     3061 tggaataact tccaacgatg tcgagagaat cggaaaatac tttaacggtg gaaaaaaaga
     3121 ttggttggta ttgttcaccc ttagatcagt cccaatattt ccgagctcac caatttcaat
     3181 tacttcagga ttgataaaaa ttaaaatgaa gacgtttcta atcactacat ttttgggaac
     3241 tattattaga aacctgtgtt atttatatct cggatatgcc ggtcttgcag catctgaaga
     3301 agtaatgagg gggttaaata aatccgaaac cgttggagaa ataattgttg tctctttgat
     3361 tggatttgca cttttatggg gatacaaaaa aagaaaacaa atattttcta ataatgaaaa
     3421 cgggtctagt gaaaataaaa taaaacaagc taaagatctc ttaaattaca aaacagtaga
     3481 tgagctccca aaagatgaat cagaagaatt ttcaactatc tatattttta gacacggtca
     3541 aaccgatgat aacgcaaact ttattttcag tggtaataga gacgcaaaac ttacagaaga
     3601 aggaaaaaaa caagctaaaa tactttctga gaagataaag ggtataaagt tcgacaggct
     3661 tatctcgtca ccccaaaaca gagctgtaca gacaatggaa atagcaatgt cacaaaacca
     3721 aagtgctaaa aatatgaaaa ttgaaactga tgaaagaata agagaaagat cttatggtga
     3781 tttaacggga aaaagtaaga tggaaattca attaaacaac cctgaagagt tattaaaaat
     3841 ccgaagaagt tacgatttcg tcccgacgaa tggtgaaagt atagaaatgg tatgtaaaag
     3901 agttgaggaa ttctgcgatg acttagtcaa agagatatca ggaaaaaatc ttaaggtggc
     3961 tatttcctgc cacggaaatt ctatacgtgg ctttcgaaaa tattttgaaa aattagacga
     4021 agaaacgacc gcaaagcttg aaactccgct cggacaagat tacgcttctt acgtaatatc
     4081 taactagaag taatttttag aaaataatta aaattcgtag aaaatctcaa gcaatctgtt
     4141 gtatttagct actctctcgc ccctggcggg agcccctgat ttcataaaat ctgcatctac
     4201 cgctactgca aggtccgaga taaacgaatc aacgttatca ccgcttctat gtgaaataac
     4261 gatatccatc tttgattttc gagctaactt tataaaatcc aatgtttcgg tcaatgtacc
     4321 tatttgattg ggtttaacta tcaccgcatt agccattttt tctttagcga ctctttctaa
     4381 tatttttgga ttagttacta ctaaatcgtc agccacaacc aaatatttac cagcattttg
     4441 tttataaaat ttagtccaac caaattcatc tctttcaaac ataggatctt ccaggtaaat
     4501 aattttgtaa tgcataaaga gatcttgata gtattttatt acctcgtcag aactcaactc
     4561 taaattctct tcgtcaatac aatacttccc attttcataa aaagaacctg cagcaatatc
     4621 taatccaaaa aacacatcat taccggggtc aaactggaca gaagcagcct gttcaagaaa
     4681 atccaaggca gttcttgcgg ataaacctct gggttcaaaa ccaccctcgt caccaacacc
     4741 tgtttcaaaa tcgtctttta acaaaatctt tttaagatct ttatatatac ttgctcccat
     4801 ttgtagagct ttattgtaag gagtattcaa agctggtatc accataaatt cttgaaaaga
     4861 aagtttattt tttgcatgtt ctccaccgtt aataatatta aaaataggag tgggaaattt
     4921 taaatctccg ttgagttttt gctttcccga atacattaga cttaaatatt ggtagagttc
     4981 taaaccagcc gatacagcag ccgattttgc gcacgctaac gaaacagaca aaatactatt
     5041 agcccccaac ctacttttat ttgaagtacc atccatttta ataagatttt catcaatact
     5101 tttttggtta gagggatctt tacctaccag aacgtcattg atggttgttg aaacaaaatc
     5161 ggctgccttt tctacatcta tatacacagc ctcgttttcc ccaactgaag cacccgaagg
     5221 aacgggctgc acacccacgg aaccatcatc tagagtcacc ttggtttcaa gagtccaatt
     5281 ccctctggaa tctaatatct tttgacttct aatattctca attttacgca ttttaccttt
     5341 taattacttt ttctttttat taagtacttt cttctcggct tcataaacta tctctctagt
     5401 tttttctatc acatcagggc taatagaaac cgaggtcata ccccattcca ccatcatctc
     5461 cactaattcc ggaaatactg agggagcttg tccacagacg gaacacgtga tatctctttt
     5521 tcgacaggta gtaacaattt tttccaaaga ttttaacact gcggggtcca tctcattgta
     5581 tgtatcagca actttttcat tatctctatc tacacctaaa gttaacatcg ttaaatcatt
     5641 tgtaccaatg gatacaccgt ctatcccttc gtcaataaag tcatcaagta aaattacgtt
     5701 agaaggaatt tcaaccatca tccaaaattt aaatgacccg cttcttctta aacctgaagc
     5761 tgaaacaatc ttttttactt cccttaactg ttctacagtt cttacaaaag gcatcatcac
     5821 ccaaaggttt ttcaatcctt ttttatttct aacgattttt atagcttcca gctccatttc
     5881 aaatacatct aaatccttta tatatcttga aactcctcta taaccaatca ttggatttgg
     5941 ctcatctgtt tcaaactccg ccccaccctt taaaccggca tattcgttac tcttaaaatc
     6001 ggtaaatctg tacacaaccg gtcttggatt gaacgcatcg gcaaactttt caagaccttc
     6061 tgctaatttc agtataaatt ctttcttttt cccttctttt ataaacttct tagggtgagt
     6121 gcctatattt gcaatgataa attcagctct taaaagacca attccgtctg tatttctaga
     6181 agccatttct gaagctaact ccggttctgc taaattaacg tatatttttg tggctgtttt
     6241 tattttactc aaatcgacag aggatttctt agtttcctgg actacttttg taatatcacc
     6301 ttcataaatt tttccagaag ctccgtctac agtaataatc tcaccgtttg aaagcatttt
     6361 tgtagcattt aaagtaccta caactgcagg aacgcctaac tc
//
LOCUS       DAOTFA010000024         6305 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate AMR_MDS_3115
            NODE_8850_length_6305_cov_6.039200, whole genome shotgun sequence.
ACCESSION   DAOTFA010000024 DAOTFA010000000
VERSION     DAOTFA010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868139
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (wastewater metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6305)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6305)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:36:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 536
            CDSs (total)                      :: 521
            Genes (coding)                    :: 520
            CDSs (with protein)               :: 520
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6305
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8850_length_6305_cov_6.039200"
                     /isolate="AMR_MDS_3115"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..293
                     /locus_tag="P5014_01385"
     CDS             <1..293
                     /locus_tag="P5014_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HRY56795.1"
                     /translation="ICGQLLKKGVKVFVEGRLQTRDWVGEDGKKRYKTEIIIDDMIIL
                     TPKKSGEGSEEDYPEIENHEHTEEVGPEEEINLDDLGDLDLGDLDDSTGEDK"
     gene            405..662
                     /gene="rpsR"
                     /locus_tag="P5014_01390"
     CDS             405..662
                     /gene="rpsR"
                     /locus_tag="P5014_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008826665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HRY56796.1"
                     /translation="MAYKKDKKPRREKKIIVAKNCYFTETGTKPDYKEILVLRRFINE
                     RGKILPQKYSGLTAKNQRLLALEVKKARYMGLLPYTDRHAL"
     gene            682..1707
                     /locus_tag="P5014_01395"
     CDS             682..1707
                     /locus_tag="P5014_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56797.1"
                     /translation="MALITAHFDVSGIDFLIKKTKKNTEFFNFPYVYTKGIFGNQCDQ
                     KQFYSNIVEKVMSDREIKNPSCDVLTCGFPDSPELNLKTKYSVKTLDLIESSEGIVPV
                     FINNKVFLTKGMMCSYELDEDEDFLGNENDLGEFDQIANLNAYPQTTPDDLSSQVSLD
                     SKICEKIPERFRFESGRKIVFTGGRFTQKILNPELNYILILEALRGLGVFNVYLDSSN
                     AYSLLRTVQMYDRSLVPDIESFFDTSGFFIRTGGSCECLINNGLGDDKFIEIEDNKVF
                     IYPFRSDTPARLSIKSRELGTIDVHTNGGSLGIVFDTRAPGDSIYDDVKLLNESVKQF
                     ENVLKDR"
     gene            1709..2722
                     /locus_tag="P5014_01400"
     CDS             1709..2722
                     /locus_tag="P5014_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56798.1"
                     /translation="MLVPVARKIMDNRKCFIERVFPVEGVWDVKIGDFVEPFSRLGSC
                     VFSQNKMTLPSDFKPRDIKGTEKFFYVDTLLGNVKKEKIFAPYDGTLKKLEDKKYVYE
                     ENPRKYFLLSGVWGTVKSLYGKSSALIETQSKDISFTYSTEFHSSGELVVFPNPSDIL
                     KKSYLENFVKGIKGKIIYIGNHVGLEVVKRAYELGASAVIVGSCHTDVFNFANNNGFS
                     FGVFSGFGNIRTPEEIYKYLSSVSYRYVFFEGAKNILRIPVREEDMKILNKKEKITDI
                     NIINPEYVKNVEPGMRVQALQRPYFGWFGVVDRVSEFSIFVKFGSDERTVEIRLPNFL
                     IIE"
     gene            2726..3940
                     /locus_tag="P5014_01405"
     CDS             2726..3940
                     /locus_tag="P5014_01405"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRY56799.1"
                     /translation="MKFQKFQRILITFLIAVSFFYGGYYFGKRGFVFEVRKNPPQIEI
                     TNKLPANGELDFSLFWEVWDMLNTTYLERPIDQKAMMYGAISGMVQALGDPYTSFLPP
                     AVNESFMDSMGGKYEGIGAELSIKDGNLIIVSPFDGSPAKNAGARPGDKILQIEQTST
                     AGMSTSEAVALIRGDSGTTVKLVLQTGNSDPREVTITRGVITVASVTWEDKGDGTAYI
                     RISRFGTDTNNEWDKAVSELNVKMEELDAVIVDVRGNPGGYLESAVHISEDFFRGKTV
                     LYEETSVGKQIPFETSKVGVFKDVPAVFVLIDQGSASASEILAAALKDNIGAKLIGAK
                     SFGKGTIQDAKDFKDGSGIHITIAKWLTPNKVWVHGKGIEPDVPVEITDEDLNNQIDS
                     QLNKAIEMAKEF"
     gene            complement(4012..4980)
                     /locus_tag="P5014_01410"
     CDS             complement(4012..4980)
                     /locus_tag="P5014_01410"
                     /inference="COORDINATES: protein motif:HMM:NF015427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PCRF domain-containing protein"
                     /protein_id="HRY56800.1"
                     /translation="MNQDYLKIEIEKIEKEIQINKEVLQKETNDDMKSLIEKEILDLE
                     KQKKDFEESIYNETVFDGSDNNGESEGNTDIDPNTIMLEIRSGTGGDEAGLFARDLYR
                     MYSRYGEKNKWKISEDFVNENEAGGIKTLIAEIKGNNIYNLLKYESGVHRVQRVPVTE
                     AAGRIHTSTATVAVLPKIKKIDIEIKPEDLKWDFFRSGGKGGQNVNKVSTAVRLTHIP
                     TGTIVECQEERYQGKNREKALQLLHSKLYTQMQEQSVKNITDLRSSQVGSAERSEKIR
                     TYNFPQDRITDHRINKTWHSIDKVMNGEIDNILKDTTVEMEKNLEN"
     gene            complement(5061..5414)
                     /gene="rpmE"
                     /locus_tag="P5014_01415"
     CDS             complement(5061..5414)
                     /gene="rpmE"
                     /locus_tag="P5014_01415"
                     /inference="COORDINATES: protein motif:HMM:NF000612.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HRY56801.1"
                     /translation="MKKDIHPKLNKECVVTCACGNTFTTISTLPSITVEICSACHPFY
                     TGQSKFVDTEGRIDKFSRRLKVAEEKKKVAEEMKSVKKSKNKGSEEYKEVSLKEMLQK
                     AKAEEEKSAKKEDSE"
     gene            complement(5458..>6305)
                     /locus_tag="P5014_01420"
     CDS             complement(5458..>6305)
                     /locus_tag="P5014_01420"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRY56802.1"
                     /translation="VCIVLLPYTIYEIQKVKIHHKDYFNLILLGIFSQTSLGLIFIGL
                     KYTTATDNAIIGILGSILSVIAGHYFYNEKIHKDLKIGLWLASLGTLVVVIEPLFSQS
                     QNINILERIFGNTLILIYNFFWVTYIVWSKMSMGERSKILEKTLGFIKIKPMTKEYPP
                     TLLIAISMYVGLFTIAPMAVFEMMGSFGNNQIFNLSQMGFEAVIGLLYMALFSSIVAY
                     ISYQKAIKSVKVSDIAFFHYLSPLFTLPVAYVILGEIPNKFVIAGSVFIALGVYIAEI
                     KNNKD"
BASE COUNT         2043 a          960 c         1186 g         2116 t
ORIGIN      
        1 agatttgtgg tcaattgtta aagaagggtg tgaaggtttt cgtagaggga agactccaaa
       61 ccagagattg ggttggagaa gatggaaaga aaagatacaa aacagaaatt atcattgacg
      121 atatgataat tttaactccc aaaaaatctg gggagggttc ggaagaagat tatcctgaaa
      181 ttgaaaatca cgaacatacc gaagaagtag gtccggaaga agaaataaat ctcgatgatt
      241 taggagactt agatcttggg gatttagatg attcgacagg ggaggataaa taatttagat
      301 ttttgatgga taagggtgcc ccgccgaagg cggggcaccc atccactaaa ttttttcttt
      361 agtttttaat acctaaacat taaaagaaaa ttaattaaat aaaaatggca tacaaaaaag
      421 ataaaaaacc aagaagagaa aagaaaatta ttgtagctaa aaactgttat tttactgaaa
      481 ccggtactaa acccgattac aaagaaatat tagttttaag aagatttata aatgaaagag
      541 gaaaaattct tcctcaaaaa tacagtggtc tgactgctaa aaaccaaaga ctattagcac
      601 ttgaggtcaa aaaagcgcga tatatgggac ttttgccata taccgataga catgcgcttt
      661 agtcattgat atcatataaa catggctcta ataactgctc actttgatgt ttcaggtatt
      721 gatttcctga ttaaaaaaac caaaaaaaac acagaatttt ttaattttcc ttatgtttat
      781 accaaaggaa tttttggtaa tcagtgcgat caaaaacagt tttattcaaa catagtagag
      841 aaggttatgt ccgatagaga gataaaaaat ccttcctgcg atgtattaac atgtggtttt
      901 cccgattctc ctgaattaaa ccttaaaact aaatattccg tgaaaacctt agatttgata
      961 gaaagttctg aaggtatcgt acctgttttt ataaataata aagttttcct aactaagggt
     1021 atgatgtgtt cttacgaatt ggatgaggat gaagattttt taggaaatga aaatgacctt
     1081 ggggagtttg atcagattgc aaatctaaat gcataccccc aaacaactcc tgatgattta
     1141 tcctcacagg ttagtcttga ttcaaaaatt tgtgaaaaga taccggagag atttcgtttt
     1201 gaatcaggaa ggaagatagt ttttacggga ggaagattta cccaaaagat tttaaatcca
     1261 gagttaaact acattttgat tttagaggct ttgcgtggcc tgggagtctt caatgtttat
     1321 ttggattctt ctaatgctta ctccttgtta agaacagtgc aaatgtatga tagaagttta
     1381 gttcctgata ttgagagttt tttcgatact tcaggttttt ttatacgtac aggcggttct
     1441 tgcgaatgtt taattaacaa tggtttggga gatgataagt ttatagaaat cgaagataac
     1501 aaggttttta tatacccttt tagaagtgat actcctgcta gattatcaat aaaaagccgg
     1561 gagttgggta cgattgatgt acataccaac ggaggctctt taggcatagt gtttgacacc
     1621 agagcaccgg gggatagtat atacgacgat gttaagttac taaatgaaag tgtaaaacaa
     1681 tttgaaaatg ttttaaagga taggtgatat gttggtacct gttgcaagaa aaataatgga
     1741 taatagaaaa tgttttatag aaagggtttt tcctgtagag ggggtgtggg atgttaagat
     1801 aggcgatttt gtagagccgt tcagtcgtct tggaagctgt gttttttcac aaaataaaat
     1861 gactttgcca tccgatttta aaccccgtga catcaaaggt actgaaaagt ttttttatgt
     1921 tgatacactt ctaggtaacg ttaaaaaaga aaaaatcttt gctccgtacg atggtacact
     1981 taagaaatta gaagataaaa aatatgttta cgaagaaaat ccaagaaaat actttttgct
     2041 ttcaggagtg tggggcactg taaagtcgtt atatggtaaa tcctctgcat tgatagaaac
     2101 acagtctaag gatatttctt ttacttacag cactgagttt cattcatcgg gggagttagt
     2161 ggtttttcct aatccttcgg atattttgaa aaaatcctat ttggagaatt ttgttaaagg
     2221 tataaaaggc aaaattatat acattggcaa tcacgtaggg ttggaggttg ttaaaagggc
     2281 ttatgaactt ggtgcttctg ctgtcattgt aggaagctgc catacggatg tctttaattt
     2341 tgctaataac aatggttttt cattcggtgt tttttcagga tttgggaata tcaggactcc
     2401 tgaagaaata tataagtatt taagttccgt ttcttataga tatgtttttt ttgagggagc
     2461 taaaaatatt ttaagaatac cggtaagaga agaggacatg aagatcttga ataaaaagga
     2521 aaagataacc gatataaata ttattaatcc tgaatatgtt aaaaatgtcg aaccgggaat
     2581 gagggtacag gctctgcaaa gaccgtattt tggttggttc ggggtagttg acagggtttc
     2641 tgaatttagt atatttgtta agttcggttc agatgaaaga accgtggaaa ttaggttgcc
     2701 gaattttctg attattgaat aaaaaatgaa attccagaaa tttcaaagaa ttttaataac
     2761 atttttaata gctgtaagct ttttttatgg tggatattac tttggaaaaa gaggttttgt
     2821 ttttgaagtt agaaaaaacc ctccacaaat agaaattacc aacaaactac ccgctaacgg
     2881 agaattggat ttttccttat tctgggaggt ctgggacatg ctaaatacca cttatttaga
     2941 aagacctatt gatcagaaag ctatgatgta tggtgctatt tcggggatgg tacaagcctt
     3001 gggtgatccc tatacttctt ttttacctcc ggcagtaaat gagagtttta tggattctat
     3061 gggaggaaaa tatgaaggaa ttggagccga gctaagtata aaagatggca atttaataat
     3121 cgtttcacca tttgatggct ctccagcaaa aaatgccggt gccagacctg gagataaaat
     3181 tttacaaatt gaacaaacaa gcacagccgg aatgagtaca agtgaggcgg ttgctttgat
     3241 tagaggagac tcaggaacta cagtaaaatt agttctacag accggaaata gcgatccgag
     3301 ggaagttact atcacaagag gtgtgataac tgttgcaagt gttacatggg aagataaagg
     3361 tgacggtacc gcatatatta gaataagtag atttggtact gatactaaca acgagtggga
     3421 taaagcagtt tcggaattaa atgtaaaaat ggaagagttg gatgctgtga ttgtagatgt
     3481 tagaggaaat cccggaggtt atctggaatc agcagttcat attagcgaag acttttttag
     3541 aggaaagaca gttttatatg aagaaacatc ggtaggtaaa caaattccgt ttgaaacatc
     3601 taaagttgga gtttttaaag acgttccggc ggtattcgta cttattgacc agggaagtgc
     3661 ttccgcttca gaaatattag ccgctgccct taaagataat attggggcaa aattaatagg
     3721 ggctaaatct tttggaaagg gaactattca agatgcaaaa gattttaagg atggatcggg
     3781 tattcacata acaatagcca aatggctcac accaaataaa gtttgggttc acggaaaggg
     3841 tatagaaccc gatgtacctg ttgaaataac agatgaagat ttgaataacc aaatagattc
     3901 ccagcttaat aaggcaattg aaatggctaa agagttttaa acttatcagc gtctgtcaga
     3961 aaatttgtta aataatccct taaattactt ttactagaaa ggcaagaaat tttaattttc
     4021 caagtttttt tccatctcaa cggttgtatc ttttaatata ttatctattt ccccattcat
     4081 aactttatct atactgtgcc atgttttatt tattctgtgg tctgtgattc tatcctgagg
     4141 aaagttatat gtcctaattt tttcacttct ttcggcagaa cccacttgag atgatcttaa
     4201 atcagtaata tttttaacac tttgttcctg catctgggta taaagcttgg aatgaagcaa
     4261 ctgcagtgct ttctccctgt ttttaccctg atatctttct tcttggcatt caactattgt
     4321 gcctgtagga atgtgcgtca gtctaactgc tgtcgatact ttgttcacat tttgtcctcc
     4381 ttttccgcct gatctgaaaa aatcccattt taaatcttcc ggtttaatct caatatctat
     4441 ttttttaatt ttaggtaaga ctgctacggt tgcagtagat gtgtgaatcc ttccagccgc
     4501 ttcagtaact ggaactcttt gaactctgtg tactcctgat tcatatttta ataaattata
     4561 tatattatta ccttttattt ctgcaattag ggttttaata cccccagctt cattttcatt
     4621 aacgaaatcc tcacttattt tccatttatt tttttcacca tatcgtgaat acattctata
     4681 taaatcacgt gcgaaaagac ctgcttcatc accaccggta cccgatctta tttctagcat
     4741 tatcgtattt gggtctatat cagtgttacc ttcgctttca ccgttgttgt cagatccatc
     4801 aaaaacagtt tcattataga tagattcctc aaaatctttt ttttgctttt ccaaatccaa
     4861 tatctctttc tcaattagcg acttcatatc atcgtttgtt tctttttgta aaacttcctt
     4921 gtttatttga atctcctttt caattttttc tatttcgatt tttaaataat cttgattcat
     4981 attttgtaag aaaataggta caacataatt atataactaa aaaaatcgga aattttaata
     5041 ctattggttg agttagtaat ctactctgaa tcttcttttt ttgctgattt ttcttcttcg
     5101 gctttagctt tttgcaacat ttcttttaac gatacttctt tgtattcttc tgaacctttg
     5161 ttcttggatt ttttaaccga tttcatctct tccgcaactt tctttttttc ttcagcaacc
     5221 tttagtcttc ttgaaaattt gtctattctt ccttctgtat caacaaattt agattgtcct
     5281 gtgtaaaaag gatgacacgc actgcatatt tcaactgtta ttgatggtag agtagagata
     5341 gtagtaaagg tgttaccaca agcgcatgta accacgcact ctttattcaa ttttggatga
     5401 atatcctttt tcataacgct aaggagtata acaggatttt ttttattcaa caagtcctta
     5461 atctttattg tttttaattt ccgcgatata cactccgaga gcaatgaaaa cagatccagc
     5521 aatgacgaat ttattgggta tttcacctaa aattacatat gctaccggta acgtgaacaa
     5581 agggcttaga taatgaaaaa aggctatgtc agacactttt acggatttta tggctttctg
     5641 gtagctgata taggctacaa ttgatgaaaa taatgccata tataaaagtc ctatgactgc
     5701 ctcaaaaccc atttggctta aattgaaaat ttgattattt ccgaaggaac ccatcatttc
     5761 gaatacagcc ataggagcta ttgtaaaaag tcccacatac atcgatattg ctattaagag
     5821 agtaggtgga tactccttag tcataggttt aattttaata aaaccaagtg ttttttctaa
     5881 aatctttgat ctttctccca tactcatctt tgaccaaact atatatgtaa cccaaaaaaa
     5941 gttatagata agtattagcg tgtttccaaa aatcctttct agtatattga tgttttgaga
     6001 ttgtgagaat aagggctcta tgacaactac caacgttcct aaggatgcaa gccaaagtcc
     6061 gatttttaaa tctttatgaa tcttttcatt ataaaagtaa tgaccggcta ttacagataa
     6121 tatagatcct aaaattccta tgatagcgtt gtcagtagct gtagtgtatt ttaatccaat
     6181 aaaaatgaga cccagagatg tttgtgaaaa tatgcctaat aatattaaat tgaaataatc
     6241 tttgtgatga attttaacct tttgaatttc gtaaatagtg tagggtagaa gtactataca
     6301 aacga
//
LOCUS       DAOTFA010000025         6116 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate AMR_MDS_3115
            NODE_9227_length_6116_cov_5.602046, whole genome shotgun sequence.
ACCESSION   DAOTFA010000025 DAOTFA010000000
VERSION     DAOTFA010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868139
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (wastewater metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6116)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6116)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:36:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 536
            CDSs (total)                      :: 521
            Genes (coding)                    :: 520
            CDSs (with protein)               :: 520
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6116
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9227_length_6116_cov_5.602046"
                     /isolate="AMR_MDS_3115"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..252
                     /locus_tag="P5014_01425"
     CDS             <1..252
                     /locus_tag="P5014_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56803.1"
                     /translation="AGNEQFGIRLSSSGGNGNPLYPYDDSILYAFNATQTTSDAIAED
                     PDGDDIPTEYSIYYLSNISGGTEVSDYSTSITYIVASNF"
     gene            314..1426
                     /locus_tag="P5014_01430"
     CDS             314..1426
                     /locus_tag="P5014_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56804.1"
                     /translation="MKKINQHILKIFSITAVLFLVSIYPNKALAEPLEDRSDTMSRLG
                     SSSAANVTSDHTIQFVTPSGVASGETIVIDFPSDFNGSTHPSGALDYTDIDLFIDDVP
                     DGTCDGTAQTIVGATPGVSDWLAEFSGTDNRTLTITSGGASAVVAAGYEVCIEIGQNA
                     EGGAANSQYINPTTIGSFMIGLEAGVDTGDVSVSIIANDVVVITAKVNETITFAISDY
                     DIGFGVLSLSNARFATGTEPYGADGPTPVSAHTMSVATNGSSGYDITYKGLTLESGLD
                     DIDVATITGDGDGVPGSEQFAISFTTSGNATIASAYQQASSNYSFVETTETSFASETG
                     PTATETLSAFYLANISGLTPAGTYSTAITYVASANF"
     gene            1432..2721
                     /locus_tag="P5014_01435"
     CDS             1432..2721
                     /locus_tag="P5014_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56805.1"
                     /translation="MPKSVKKYNKLLLVLGVVLFSLVSFSLKNRAYALTQPTKVEIDG
                     EPGKVLTGTLELYNEQEQEITFYLSYQNFEARGEGGSPSFLDYDGTGLASWIKTAQSV
                     TLKPKERIEFPYEIQIPQDMEPGTYLAAIFFGNTPPQAMEGGQVTIGTKLGTLLFLNL
                     PGAKVEGAGVTEFSTEGGNFFSYLPLNLFYRFSNNGALKVMPFGSIDIKCLFGRTVGT
                     LDANISKGNVLPNSVRKFVITWDRLKQDNVNEKEDKTEEVKMEPGQKLSFFDAVKVQI
                     KNFTFGKYTATLNVNYGDNLSATKETSFWVIPWQLLSLVIAALVLIIMIFRGMMKRHN
                     KKLIEMVKKEALTSQQVTIAPTESIVKEEKKTREKVKKEVKEEVEEIKETAEDLKENV
                     EEIKEEVEEKIEEKTEEEKVDNDSETEEKLTALNPKE"
     gene            2894..4009
                     /locus_tag="P5014_01440"
     CDS             2894..4009
                     /locus_tag="P5014_01440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56806.1"
                     /translation="MKKQNFNSIKIFPTLVLVILFSHATKVLADPLQNKSDAMSRLGN
                     SSGDAVLSDHEIQFTTPTGVASTQSIEITLPSDFDGDSDPQGALDYTDVDLLIDNTPD
                     GICDGIDQTLVGAGAGASEWDAVFSGTENRVLTITSGGASAIVGLGDEVCILIGENAT
                     GGTANSQYINPSASAAYTISIVSASDSGEISVSIIDNDTVVITADITETISFAIDDYT
                     VGFGNLTLSTARFATGSAPYGSNPPSVAAHHMTVATNGSTGYEITYLGSTLTNQNNDT
                     IAPATIVGDEDGTPDTEQFAIGFTTDGNASIASAYNQVSPNFNYSFVANVETPFASEA
                     IPTTTETISAFYLANISGVTPAGTYSTALTYVATANF"
     gene            4018..5199
                     /locus_tag="P5014_01445"
     CDS             4018..5199
                     /locus_tag="P5014_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56807.1"
                     /translation="MIKTKNCPKLILVLTVFFFSLFYIFSEQPAHALTVPTKLEIEGD
                     PGQKLFHELEIYNEGDQEVTYYISAKNFEARGETGSPYFLPDTSTGLASWIFSAESVV
                     VGPKERIKFPYEIRIPQDAEGGGYFASIFFGTTPPILENDEQVALGSKTGTLIFLNVS
                     GDVLEKAGVVEFKTKGGNFFSYLPITMYYRFSNDGGRKLIPTGTIDIKGLFGKKIATL
                     DANNSKGNVLPKSVRRFEVVWSELNQNNVIGKNKVESKETMAGSQDSKIGFFEAVKMQ
                     KENFVIGRYKAYLNLKYGDNKTSENQVVFWVIPWQILTLIVSALVLLVTIIKTMMKRY
                     NKKLTERVKKETLTSQRVNIIPTETVTEKEEETKEELKENTEDSLDADTGDPEDINPK
                     E"
     gene            complement(6022..6111)
                     /locus_tag="P5014_01450"
     tRNA            complement(6022..6111)
                     /locus_tag="P5014_01450"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6075..6077),aa:Ser,seq:gga)
BASE COUNT         2086 a         1147 c         1254 g         1629 t
ORIGIN      
        1 gcgggaaatg agcagtttgg tataagactg tcttcgagtg ggggtaatgg aaatcctcta
       61 tatccatacg atgacagcat tttatatgct ttcaacgcaa ctcaaacaac ctctgacgct
      121 attgcagagg atcctgatgg tgacgatatt ccaacggagt attctatata ttatttatcc
      181 aatatttcag gaggcacgga ggtgagtgac tactctacaa gtattaccta catagtagcc
      241 agtaattttt aatttaaatg aaagcaaaaa gtatttgaca actatacaca ttaatgttgt
      301 attattaggt acgatgaaaa aaataaacca acacatttta aaaatatttt ctataactgc
      361 cgttttattt ttagtttcaa tctacccaaa caaagctctc gcagagcctt tggaagatag
      421 atccgataca atgagcagac ttggaagttc atctgctgct aacgtaacat cggaccatac
      481 aattcagttt gttacacctt cgggtgtggc atctggagaa actattgtta ttgatttccc
      541 atcagatttt aacggatcaa ctcatccaag tggagctttg gactacacag atatagatct
      601 ttttattgac gatgttccag atggaacatg tgatggtaca gcgcaaacaa ttgtaggagc
      661 aacaccagga gtaagtgatt ggttagcaga attcagcgga acagataata gaactcttac
      721 aattacatcg ggaggggctt cggcagtcgt tgcagcaggt tatgaggtgt gtattgaaat
      781 aggacaaaac gctgaaggag gcgccgctaa ctctcagtat ataaacccaa ccacaatagg
      841 atcttttatg ataggtcttg aggcaggtgt tgatacaggg gatgtttcag taagtataat
      901 tgcaaatgat gtggttgtca ttacagcaaa agtaaacgaa acaattacgt tcgctataag
      961 tgactacgat attggatttg gagtactaag cctatcaaat gcaagatttg caacaggaac
     1021 agagccttat ggtgctgatg gaccaactcc tgtttccgca cacactatgt cagttgccac
     1081 aaacggaagc tcgggttacg acatcactta caagggtctg acattagaat ctggtttaga
     1141 tgatatcgat gtagcaacaa taacaggaga cggagacgga gtacccggct cagaacaatt
     1201 cgcaattagc tttacaacaa gcggtaatgc cacaattgca tctgcatatc aacaggcatc
     1261 gagcaactac tcgtttgttg aaaccacaga aacatccttt gcatctgaaa caggtcctac
     1321 tgctactgaa acattatcag cgttttattt agcaaatatt tcgggattaa cccctgccgg
     1381 aacatatagt acagctataa cctatgtggc atcggcgaac ttttaaacca catgcccaaa
     1441 tcggtaaaaa aatataataa attactttta gtacttggag tcgtattatt ttcactggtt
     1501 tccttttcat taaaaaacag ggcgtatgct ctgacacaac caacaaaggt tgaaattgac
     1561 ggtgagcccg gaaaggtcct cacaggtacc ttagaacttt acaatgaaca ggaacaggag
     1621 ataactttct acctttccta tcaaaacttc gaagccagag gagaaggcgg ttctccatcc
     1681 ttcttagact acgatgggac tggcttggca tcttggatta aaactgcaca gagtgttact
     1741 ttaaaaccga aggaaagaat agagttccct tatgaaatac aaattccaca ggatatggaa
     1801 cccggaacat atttggcagc tatatttttt ggaaacacac ctcctcaagc gatggaaggc
     1861 ggtcaagtta caataggaac aaaacttgga acattgttat ttttaaatct tccaggtgca
     1921 aaagttgaag gtgctggagt tacggaattt tctacagagg gcggcaactt tttttcatac
     1981 cttcctctaa atctcttcta cagattctca aataacggtg cgttaaaggt tatgcctttc
     2041 ggtagcatag atattaaatg tctttttggt agaacagtag gaacattaga cgcaaatatt
     2101 tcaaaaggaa atgttttacc aaacagtgta aggaagtttg ttatcacctg ggacagattg
     2161 aaacaagaca acgttaatga aaaagaagac aaaacagaag aagttaaaat ggaacccgga
     2221 caaaagttaa gtttctttga cgctgttaaa gtgcaaatta aaaactttac cttcggtaaa
     2281 tacactgcaa cattaaacgt gaactacggg gataatctaa gtgcgacaaa agaaacgtct
     2341 ttttgggtaa taccttggca actactctct ctcgtaatcg ctgctcttgt tttaataata
     2401 atgatattca gaggaatgat gaaaagacat aataaaaaac ttatagagat ggtaaaaaag
     2461 gaggcgttaa catcacagca ggtcacgatt gctcctacag agagtatagt taaagaagaa
     2521 aagaaaacac gtgaaaaagt aaaaaaagaa gttaaggagg aagtcgaaga gataaaagaa
     2581 accgcagaag atttaaagga aaatgtagag gagattaaag aagaggttga agaaaaaatt
     2641 gaagaaaaga ccgaagaaga aaaagtagat aatgactcag aaaccgaaga aaaacttaca
     2701 gctttaaatc ctaaagaata gtataattaa aaaacgtaag gacaagcaaa aggcccgcct
     2761 ccggcgggcc tttttgatat ttaattttga tgtgtgatac tgtctttcta tatcataaaa
     2821 atcccggggg aaaatttaag aatgaataaa gtaattgacg gggtatgact gctctgtaga
     2881 attattagct gtaatgaaaa aacaaaactt taattctata aaaatatttc ctactcttgt
     2941 tctagttatt ttattttccc atgctactaa agttctggca gaccctttac aaaataagtc
     3001 tgatgccatg agcaggctgg gtaattcaag tggtgacgcc gtactatcag accacgagat
     3061 acaatttact acaccaaccg gagtagcttc aacacaatct atagaaataa ccctcccttc
     3121 tgattttgat ggggacagcg acccacaagg agcacttgac tatacagatg tcgacttact
     3181 tatagacaac acaccggatg gaatctgtga tggaatagat caaacattag taggagcagg
     3241 tgccggggcc agtgagtggg atgccgtttt tagcggtaca gaaaaccgtg tactcactat
     3301 cacttcaggt ggagcttcgg caattgtagg attaggagat gaagtgtgta ttctaatagg
     3361 agaaaatgca acaggaggga cagctaattc tcaatatata aacccatcag caagtgctgc
     3421 ttacaccatt tctattgttt ctgcatctga ttcaggagaa atctctgtca gtataattga
     3481 taatgataca gtagtcataa cagcagatat tacagagaca atatcgtttg caattgacga
     3541 ctacaccgta ggatttggta acttaacctt atcgactgca agatttgcaa cgggaagcgc
     3601 cccttatgga tcaaatcctc cctcagttgc agctcaccac atgaccgtcg ccactaacgg
     3661 aagcacaggg tatgaaataa cttatttagg ttcaacactt accaatcaaa acaacgatac
     3721 aatagcccct gcaacaattg taggagatga ggacggaaca ccggatacag aacaatttgc
     3781 aataggtttt actacagatg gtaacgcatc aattgcctca gcatacaacc aagtatcccc
     3841 taactttaac tatagcttcg tcgcaaatgt agaaacgcca tttgcatctg aagctattcc
     3901 tacaacaaca gagactatat ctgcattcta cttggcaaat atttcaggag taactcctgc
     3961 tggaacatac agcacagctc ttacgtatgt tgccacagcg aatttttaaa ataaattatg
     4021 ataaaaacaa aaaactgccc aaaattaata ttagttctca ccgttttctt tttctctcta
     4081 ttttatatat tttcagaaca acctgctcat gcacttactg tacctacaaa gcttgagata
     4141 gaaggagatc ccggtcaaaa actttttcac gaactggaaa tttacaatga gggtgaccag
     4201 gaggttactt actatatttc tgccaaaaat tttgaggcaa gaggagaaac aggttcgcca
     4261 tattttttac ctgatacaag cacaggcttg gcatcgtgga tattttcagc tgaaagtgtt
     4321 gtcgtgggac caaaagagag aattaaattt ccatatgaaa ttaggattcc acaagatgct
     4381 gagggcggag gttattttgc atcaatattt ttcggcacta caccaccaat ccttgagaat
     4441 gatgaacaag ttgccctggg tagcaagact ggaacactta ttttcttaaa tgtatccggc
     4501 gatgttctag aaaaagcagg tgttgtcgag tttaaaacca agggtggaaa tttcttctct
     4561 tatcttccaa tcacaatgta ttatagattt tcaaatgacg gaggaagaaa gttaattccc
     4621 acaggaacaa tagacataaa aggtttattt ggtaaaaaga tagctacttt agatgcaaat
     4681 aattcaaaag gcaatgtttt accaaaaagt gtaagaaggt ttgaggtagt ttggagcgag
     4741 ttaaatcaaa acaatgtaat aggaaaaaac aaggtcgaaa gcaaggaaac gatggccggg
     4801 agtcaggata gcaaaatagg gttctttgag gcagtaaaaa tgcaaaaaga aaattttgta
     4861 ataggaaggt acaaagcgta tcttaacttg aaatacggag ataataaaac atcagaaaat
     4921 caggttgttt tttgggttat tccctggcaa atacttactc ttattgtaag tgccttggtg
     4981 cttttagtaa cgatcatcaa aacgatgatg aagaggtaca acaagaagct cacagagagg
     5041 gttaaaaaag aaaccttaac atcacaacgt gtgaatatta ttccaaccga aacagtaaca
     5101 gaaaaagaag aggaaacaaa agaggagctg aaagaaaaca cagaggattc tttagatgcg
     5161 gatacaggtg atccggaaga tattaatcct aaagagtaag tatcattata aagtgcaagg
     5221 acaagcaata ggcccgcctc cggcgggccc tcctttttaa cattcaaaat gtttttatca
     5281 aaatctttac aaagatagaa ttttatcagt cgctacagac agaacgatta gagttagtgg
     5341 tcttccctag gaggtctttg atccaaaaat atcactactg caacacctac cgcaattact
     5401 gcaaaggtaa ggtaaacgat agccaatatg tttaagttca tgatgaaatc ctttctaatc
     5461 ttaaggataa ccaccagaga aaaaatgtca atatagcacc acccgatata acaggaacaa
     5521 tatccttaga atttgaaaaa ttgagtaaag gacttaaaat cagagatcct aaaaacacct
     5581 gaccagcgtt gtcgaatatg ttggagaacc ttttgacctg actacgagta aataaaaaca
     5641 taagaaatta ataccacgga gaaatattta taaaataacc agtgttctac tagaaataga
     5701 ataaagataa aaatacatcg cggagggaga gggattcggt caccggttct cgcctgctgg
     5761 ctcgaccgtc taccccggct cggcgccgtc gcgcctgcgc cattcgaatc ctttttgaaa
     5821 ttcttttaag atacgcggtg atttccaaac agctttaagt ggttctcact ggcggaggga
     5881 gagggattcg gtcaccggtt ctcgcctgct ggctcgaccg tctaccccgg ctcggcgccg
     5941 tcgcgcctgc gccattcgaa tcctttttga aattctttta agatacgcgg tgatttccaa
     6001 acagctttaa gtggttctca ctggcggagg gagagggatt cgaaccctcg ataggatttc
     6061 tcctatacag tctttccagg actgcgcact agtccactat gcgatccctc caaggc
//
LOCUS       DAOTFA010000026         6067 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate AMR_MDS_3115
            NODE_9327_length_6067_cov_7.211910, whole genome shotgun sequence.
ACCESSION   DAOTFA010000026 DAOTFA010000000
VERSION     DAOTFA010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868139
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (wastewater metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 6067)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6067)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:36:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 536
            CDSs (total)                      :: 521
            Genes (coding)                    :: 520
            CDSs (with protein)               :: 520
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6067
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9327_length_6067_cov_7.211910"
                     /isolate="AMR_MDS_3115"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(36..275)
                     /locus_tag="P5014_01455"
     CDS             complement(36..275)
                     /locus_tag="P5014_01455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56808.1"
                     /translation="MQNIQKLFWKTEQRKIKDLIPFDKNPRILSDFQKDKLKESLEKF
                     NLVEIPAINTDNKILAGHQKIKIMLLLNRGDEEIV"
     gene            complement(341..1027)
                     /locus_tag="P5014_01460"
     CDS             complement(341..1027)
                     /locus_tag="P5014_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56809.1"
                     /translation="MKYGEARKLIFDNKEKEVILDLVKSAQEQISKIPKSFFQINASV
                     LQAYDTQVKMLSAVADSEFVLNAFKAAQKQIEIFSSLNNNFLYTNRPIEYKAILPIKS
                     NGEIEEVKELKAKIDSLEDKLKFYKSKENKFEIEITPTGEFFYKGKELCITTNSLAGK
                     FFKCSLEDGNNFVSDHYRFNELKCDQIKNLKRDVNNKYLKKDGLKAIMHRSGDGAGYI
                     LTEIIELGSL"
     gene            1104..3164
                     /locus_tag="P5014_01465"
     CDS             1104..3164
                     /locus_tag="P5014_01465"
                     /inference="COORDINATES: protein motif:HMM:NF023203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56810.1"
                     /translation="MEYGSSEKFSKINPQAEIISKFEPSQQSEINQKQQILSSLAYFI
                     GKDFRIPVELNEPGAGWHWDFAENKIRIDPKDLVEKPMDYLRFVISHEGGHRRISRTD
                     DIPKEIWKQPGFSFMMNAVEDPRTNNFVAEAYPRFRDQMNIAYQMDLDFEGKTKEKAK
                     KDLGYTPRFMQAGLEYIKQWYQETTGVTTPTLSQDLSEDVKNVVTKTLDSARDSWLRY
                     PSKQEADQSEPLIRQYANVSYEINRDEIWPEFKKLVDADMEDQKVQEMLKDMQKGGEN
                     GDGQQGLPQELKENLSEEEQQQLEEAIQNGQLKPGEGDPSQNSPPIDLDSLSDDLKKK
                     IKDYIDSLPDDVKKELAKKAQQTLSDFEKTINEEFEGKLSDNPDKKAERESKTPSPNK
                     PELKKESKKTQNPPDSEDQKKFRDLIEQTLKSDENIYEQMRHEVLPLIDNLENDLREI
                     FVQRRSQKWESGFRSGRKIDIKRRIQEKAKGLSAVESKAWQKRELPQEKDYAISLLVD
                     LSGSMRGEKIRETFKAAVVLAEVLNRLSINTEILGFNDRLYEYQPFGENMSKGVRENI
                     GGMLQEVDTPAAAYNDDGWAVQQASERLSHQKAVEKFLFVLSDGLPEESNKHPGSSYD
                     LETIINGVLKETDQKLIGLGIGSGTEHVERYYPNSVANIGVKEMSEKLADVIREAIAN
                     YDTF"
     gene            3177..3581
                     /locus_tag="P5014_01470"
     CDS             3177..3581
                     /locus_tag="P5014_01470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56811.1"
                     /translation="MNSDLIKFIEYSVSQKEVSLIITKDKEEIQRVAKILIELGYHEE
                     INVSDMVKLVSQPSKVFITLEDDFSKDIYDFVVQYPTGQVEIYDKVNLKSQTVTPAYK
                     DVSIIVVADEETIKKSKFPILEKVGLTYRGNL"
     gene            3578..5527
                     /locus_tag="P5014_01475"
     CDS             3578..5527
                     /locus_tag="P5014_01475"
                     /inference="COORDINATES: protein motif:HMM:NF019348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRY56812.1"
                     /translation="MTIDRPVSEQSIKPQIESPIKETFDSVSYLGVRLPKSQNRESTF
                     VPKREQYTDFIEDRFSLNLEKDIAVSLLQGDPLLIEGGTSIGKTTTVRKMASDLGWEV
                     HYANLNGATDVEDLMGRYIPNPNKNHPEDPEYVFVDGKVTSGLRQETGKTKVIILDEF
                     NSAAPNILIRLHEVLDSLQRGENVVLSEDASESIPVDKSTTKIVALMNPPGKGYFGRE
                     PLDPAQLRRWVYLKAPSDLPEETFSYSTDALFSLVPQQQEVSDIKTLPSRESALLPEQ
                     LQEIPGISEILSKYKEFHQGAKKLVKERKVAADQPQPFIYDDRMEPKRVRDFVLAFYN
                     GDINETFKSALNYYYVNKLDSNLDRQKLQELIRHVEYIAPQNTSQRRGVDRETTSPAE
                     SKTIVSTETLKNEQAAWKKVLGVDVEVPPLPDSVTPEVREKLKDLGMELRFIPDVNLG
                     TLADIKRDGVDEYLKNRQRLYPNWHKYETLTDSQKEDHSIGRNLEEWYWNLVKDEKVP
                     FPQLPGKWIAVETMPKPKYGDKYSSSQITDELGLEDRFNVTWNDANKAINEAEGNILA
                     AAGLPIIGEQVRMLEAIEWNLLANREGWGKTNTYEWTNTELRDGGVSDRVIVGYSDSG
                     GAAYAIWYRPGRSYDRLGFRLAVVL"
BASE COUNT         2166 a          903 c         1090 g         1908 t
ORIGIN      
        1 aattttgtat ttttgcctta tacttttgta tgaaattaaa caatttcttc atcaccccta
       61 tttaaaagaa gcattatttt aatcttttga tgcccagcga gtattttatt atcagtatta
      121 attgccggta tctcaactag attaaacttc tctaaagatt cctttaattt atctttttga
      181 aaatcactta aaattcttgg atttttatca aaaggaatta gatctttaat ttttctttgc
      241 tctgttttcc agaatagttt ttgaatattt tgcatataaa actattctct tctttgtttt
      301 atccttaaaa tagagagaaa aagatgacca gatggtgacc ttacagtgat cctagctcaa
      361 taatttcagt cagaatataa ccagccccgt ccccacttct gtgcattatt gcttttaaac
      421 catctttctt aaggtattta ttattgacat cccttttaag gtttttaatt tgatcacatt
      481 taagttcatt gaaacggtaa tgatcagaaa caaaattatt accatcttcg agagaacatt
      541 taaaaaattt acctgccaga ctattcgttg ttatacagag ttctttacct ttataaaaaa
      601 attcacccgt aggagtaatt tctatttcga atttattttc ttttgattta tagaatttca
      661 gtttatcctc tagtgaatca attttagcct ttagttcctt tacttcttct atttctccat
      721 tacttttaat tggcaaaata gctttatatt ctattggtct gtttgtatat aaaaaattat
      781 tattaagaga gctaaaaatt tcaatttgtt tttgtgccgc tttaaaggcg tttaagacaa
      841 attctgaatc agctactgca gatagcattt tgacttgcgt atcatatgct tgtaaaacgc
      901 ttgcgttgat ttgaaaaaat gactttggta ttttgctaat ttgttcctga gctgatttta
      961 caagatcaag aattacttct ttttctttgt tgtcaaatat taattttctt gcttcaccat
     1021 atttcatatt ttctatcctt tggctatatt ttacatctag ttactattat cggataattt
     1081 gagttaaaat aatgataatt aatatggaat atggatcatc agaaaaattt tcaaaaataa
     1141 atccacaagc tgaaataata agtaaatttg agccatctca acagtctgaa attaatcaaa
     1201 aacaacaaat tctttcatct ttagcttatt ttattggtaa ggattttaga atccccgttg
     1261 aattaaatga acctggggct ggttggcatt gggattttgc agaaaacaaa attagaatag
     1321 atcctaaaga tttggtggaa aaacctatgg attatcttcg ttttgttatt tcccacgagg
     1381 gcggccatag aagaatttct agaacagatg atattccaaa agaaatttgg aaacaaccag
     1441 gtttttcttt tatgatgaat gctgttgaag atccaagaac aaataatttt gtggccgaag
     1501 catatccaag atttagagat caaatgaata ttgcttacca aatggactta gattttgaag
     1561 gaaaaacaaa agaaaaagct aaaaaagatt taggatatac tccccgtttt atgcaagcgg
     1621 ggttggaata tattaaacag tggtatcaag aaacaacagg agtaactaca ccaacactta
     1681 gtcaagatct ttcagaagat gttaaaaatg tggtaacaaa aacattagat tcagccagag
     1741 attcttggtt aagataccct tcaaaacaag aagcagatca aagtgaaccg cttattcgac
     1801 aatatgccaa tgttagttat gaaataaatc gggatgaaat ctggccagag tttaaaaaat
     1861 tagtagatgc agatatggaa gatcaaaaag ttcaggaaat gctgaaagat atgcaaaaag
     1921 gtggtgaaaa tggagatggt caacaaggtt taccccaaga gttgaaagag aatttgtcag
     1981 aagaagaaca gcaacaatta gaggaagcaa ttcaaaatgg acaactaaaa cctggcgaag
     2041 gtgatccatc tcaaaattca cctccaatag atttagattc tctctctgat gatttaaaga
     2101 aaaaaattaa ggattatata gattctcttc cagatgatgt taaaaaagaa ctggctaaaa
     2161 aagctcaaca aacattgagt gattttgaaa aaactattaa tgaagaattt gaaggaaaat
     2221 tgtctgacaa tcctgataaa aaagcagaaa gagaatctaa gactccatca cctaataaac
     2281 ctgaacttaa gaaagaatct aaaaaaacac aaaatcctcc tgattctgaa gatcagaaga
     2341 agtttagaga cttaattgaa caaacattaa aaagtgatga aaatatatat gaacaaatgc
     2401 gccatgaagt tttacctttg attgataatt tagaaaatga tctaagagaa atttttgttc
     2461 aacgaagatc ccaaaaatgg gaaagtggtt ttagatcggg aagaaaaatc gatattaaaa
     2521 gaagaatcca agaaaaagct aaaggtttat cggcagttga aagtaaagct tggcaaaaac
     2581 gagaactgcc tcaggaaaaa gattatgcta ttagcctttt agttgattta tcgggatcga
     2641 tgaggggaga aaaaataaga gaaactttta aggcagctgt tgtattagct gaagtattaa
     2701 atagactttc aatcaacacg gaaatattag ggttcaatga tagattatat gaatatcaac
     2761 cctttggtga aaatatgagt aagggtgtta gagaaaatat tggaggaatg ctacaagaag
     2821 tagatactcc agctgctgct tacaatgatg acggatgggc agtacaacag gcatcagaaa
     2881 gattgtcgca tcaaaaagca gttgaaaagt ttttatttgt gctttccgat ggacttccag
     2941 aggaatcgaa taaacatcct ggatctagct atgatttaga aaccataata aatggtgttt
     3001 tgaaagaaac cgaccaaaaa ttaattggtt tgggtattgg tagtggtacc gaacatgtag
     3061 aacgttatta tcctaacagt gtggctaata taggagtgaa ggagatgtcg gagaaattgg
     3121 ctgatgttat tagagaagct atcgcaaatt atgatacttt ttagtatact tggcttatga
     3181 atagtgatct tattaaattt attgaatatt ctgtttctca aaaagaagtt tctttaatta
     3241 ttactaaaga taaagaagaa atccaaagag tggctaaaat acttatagaa cttggttatc
     3301 atgaagaaat aaatgtatca gatatggtta aacttgtttc ccaaccatca aaagttttta
     3361 tcactttaga agatgatttt tcgaaagata tttatgattt tgttgtccaa tatccaactg
     3421 gacaagttga aatatacgat aaagttaatt taaaatcaca aacagtaaca ccagcttata
     3481 aagatgtgtc aattattgta gtggcagatg aagaaacaat taaaaaatcg aaattcccaa
     3541 tattagaaaa agttggttta acttataggg gtaatttatg acaatagata gacctgtttc
     3601 tgaacaatca ataaaaccac aaatagagtc acccataaag gaaacttttg attcagtctc
     3661 ctatcttggt gttcgtttac ctaaatccca aaatagagaa agtacttttg ttccaaaacg
     3721 tgaacaatac actgatttta tagaagatcg attttcccta aatttagaaa aagatatagc
     3781 tgttagcttg cttcaaggag atcctctatt aatagaaggt ggaacaagta ttggaaaaac
     3841 aaccacagtc agaaaaatgg cttcagattt aggctgggaa gttcattacg ccaatttaaa
     3901 tggagcaact gatgtagaag atttaatggg acgatatatc cctaatccaa ataaaaatca
     3961 tcctgaagat cccgaatatg tttttgttga tggaaaagta acttctggtt taagacaaga
     4021 aacaggaaaa actaaagtta tcatattaga tgagtttaat tccgccgctc caaatattct
     4081 tatccgccta cacgaagttc ttgattcctt gcaaagagga gaaaatgtgg ttttatcaga
     4141 agatgcttcg gaaagtattc ccgtagacaa aagtaccacc aaaatagttg ctttgatgaa
     4201 tcctccagga aaaggttatt tcgggagaga acctcttgat ccagcacaat tgagacgttg
     4261 ggtttatctg aaagctccta gtgatttacc agaagaaact ttttcatatt ccaccgatgc
     4321 tttattttct ttggtaccac aacaacaaga agtatcagat atcaaaactt taccatcacg
     4381 tgaatcagca cttttaccag aacaacttca agaaataccg ggtatttctg aaattcttag
     4441 taaatataaa gaatttcacc aaggagcaaa aaaattagtc aaagaaagaa aagtagccgc
     4501 tgatcaaccc caaccgttta tatatgacga tcgtatggaa ccaaaacgag tcagggattt
     4561 tgtattggca ttttataatg gcgatattaa tgaaacattt aaatcagctc tcaattatta
     4621 ttatgtaaac aaattagact caaatcttga tcgacaaaaa cttcaagagt taataaggca
     4681 tgttgaatat attgctccac aaaatacatc tcaaagaaga ggtgttgata gggagacaac
     4741 ttcccctgct gaatcaaaaa caatagtttc tacagaaact cttaaaaatg aacaagcagc
     4801 ctggaaaaaa gtattagggg ttgatgtaga agtaccacca cttccagact cggttactcc
     4861 tgaggttaga gaaaaattaa aagatttagg aatggaatta cgattcatac ctgatgttaa
     4921 tttaggtact cttgccgata ttaaaagaga tggtgtagat gaatatctta aaaatcgtca
     4981 acgtctttat ccaaactggc ataaatatga gactttgacc gattcccaaa aagaagatca
     5041 ttcaattgga cgaaatttag aagaatggta ctggaatttg gtaaaagatg aaaaagtgcc
     5101 atttcctcaa ctaccaggca aatggatagc agtagaaact atgccaaaac caaaatatgg
     5161 cgataaatat tcatcgtcac aaattactga tgaactggga ttagaagatc gttttaatgt
     5221 aacttggaat gatgccaata aagcaattaa cgaggcggaa ggaaatattt tggctgcagc
     5281 aggattaccc ataattggtg aacaagtgag aatgttagaa gcaattgaat ggaacttatt
     5341 ggctaatcgt gaaggttggg gaaaaactaa tacctatgaa tggactaata ctgaattgcg
     5401 tgatggcggc gtttctgacc gtgtcattgt tggctactct gactctggtg gtgctgcgta
     5461 cgccatttgg tacaggcctg ggcgctcgta cgaccgctta ggttttcgtc ttgcggtagt
     5521 tttataggtt cttgaatttt tgaatcttga ttcttttgat ttttgtttct tggtttcttg
     5581 atccgcgaag cggtcgattt taaaatattt tttgaactat tattttgtga tgacattaaa
     5641 tagcaattac acttggtgtt taatcatttt cttagttata ctaatcatta gattgtgtta
     5701 atgaaaatat agggaaatct atatttcaca gaatcgctct tcttaaagag ctggctagtg
     5761 tccttgtagt taaggcttta gggagaatgc caattaagga gagataaaac tacttgctca
     5821 tttttaaagg tcttaatctg ataattaagc caattacaat tcaggggaaa gtctcttatt
     5881 agacgatcct tgattaagat aaattactat aaatatgtat cgaagatcgg tggtaacaat
     5941 gagtaaattt ttttactcag gaaagaaaat gggcaataca aaacgtgatg acggcgattc
     6001 taaccgtgtc attggtggca atacttttta tcttcttgat caggtaatta aaactggctg
     6061 cgacgaa
//
LOCUS       DAOTFA010000027         5935 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate AMR_MDS_3115
            NODE_9595_length_5935_cov_7.598129, whole genome shotgun sequence.
ACCESSION   DAOTFA010000027 DAOTFA010000000
VERSION     DAOTFA010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868139
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (wastewater metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5935)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5935)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:36:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 536
            CDSs (total)                      :: 521
            Genes (coding)                    :: 520
            CDSs (with protein)               :: 520
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5935
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9595_length_5935_cov_7.598129"
                     /isolate="AMR_MDS_3115"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="P5014_01480"
     CDS             complement(<1..214)
                     /locus_tag="P5014_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56813.1"
                     /translation="MKKNKQDAFLRVSLLPLLVLVGLIFGAGYFLLKDEIKLPSLRKN
                     PTIQRLEGFPTVIYTDKQIEKTRKVIT"
     gene            complement(234..1070)
                     /locus_tag="P5014_01485"
     CDS             complement(234..1070)
                     /locus_tag="P5014_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018202087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HRY56814.1"
                     /translation="MPTSIKKNPEELRKISNKLRQIVIDMLTEAKTGHTAGSLGTAEI
                     FSTLYFHILKIDPSNPNKKDRDRFILSNGHICPIWYATLSERGYFPKTELWKLRKINS
                     KLQGHPKINSLPGIENTSGSLGQGLSQAIGIALAGKMNKASYRVYCMTGDGELNEGQI
                     WESAMFAPNKKLDNLTWIIDRNYIQSDGNTEDIMPLENLRDKLESFNWYVLEINGNNI
                     EEIISACKVAESINHRPTAIIANTIPGEGVDFMENKYQWHGKVPNKKEAEEAKEKLSS
                     II"
     assembly_gap    1435..1534
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1535..1722)
                     /locus_tag="P5014_01490"
     CDS             complement(<1535..1722)
                     /locus_tag="P5014_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014791197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56815.1"
                     /translation="MENKEEKVKRPLMVCILGGAAWNEEDEPYIQAKATAKILAENGY
                     GIVNGGGPGVMRASTEGA"
     gene            complement(1712..2398)
                     /locus_tag="P5014_01495"
     CDS             complement(1712..2398)
                     /locus_tag="P5014_01495"
                     /inference="COORDINATES: protein motif:HMM:NF013030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56816.1"
                     /translation="MIIPAILEKDLKNIINKVDLVKDVAKTIQIDVLDNTLIKEKTFT
                     DLNKLKNIKTDIEIIIHLMVDDPKKYIKKLNGSFIFSKGKISNVSTFITQLTEDKNKI
                     MEFIKLAKNSGYKTGISINTDQDTSLLIPFLDKTDVIQFMGVNPGKQGNEFLPEVMDK
                     IIDFKNKFPGVETLLDGGVCRDNFISVVKTGVDKVVIGSSIFNTENPKQKFIEFSNLF
                     EEERKTAHGE"
     gene            complement(2395..2838)
                     /locus_tag="P5014_01500"
     CDS             complement(2395..2838)
                     /locus_tag="P5014_01500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HRY56817.1"
                     /translation="MIYISSDHRGLELKKYLVESLVSVGLGIVDLGPKDSDPEDDYPD
                     FAKLVAEKIQEDFENMGVLICANGVGISIAANKLDGIRAALSWNPKHAASSRNDDGTN
                     VLVLPADYITKEEALEILHTWLSTNFSGEDRHIRRIQKIAELEEI"
     gene            complement(2835..3578)
                     /gene="lgt"
                     /locus_tag="P5014_01505"
     CDS             complement(2835..3578)
                     /gene="lgt"
                     /locus_tag="P5014_01505"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HRY56818.1"
                     /translation="MINPYGILISIAIFISIKTAEGIIEKKDEDTLWGLSLWAIIFGI
                     IGARLYHVLDYLDFYIKNPVEILFLWNGGLGIFGAVLGGIGGGVIYLKVKGKKILPWL
                     DIISVVTPLGQATGRWGNFFNNEIFGKPTTLPWGIYINPENRPLMFFNNEKFHPLFIY
                     ESILNLILFIFLYRTYKRKHNKLKSGFFMSVYLLGYSVIRFSLEFLRIDPWTTHLSPS
                     LTLNVAQTISILLIILSLTFIFTNKGNKK"
     gene            complement(3578..4123)
                     /locus_tag="P5014_01510"
     CDS             complement(3578..4123)
                     /locus_tag="P5014_01510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56819.1"
                     /translation="MIKKILTYYKIPLLVSITLGITILAMSIARKPLDIAQVFVGVLL
                     GTFLLDIEFILYPYIFEPNTEYAKTVFAYIKNKDFGDLISFINEHKDEVKDKSLNSII
                     FQFVLIFLVVFTSYTNTSYFIKAFVMSVFANSIYKLTEALFEDRTKDWFWAIKGTPKK
                     EGVIFFIVGLIIVLAIALYFV"
     gene            complement(4160..4537)
                     /locus_tag="P5014_01515"
     CDS             complement(4160..4537)
                     /locus_tag="P5014_01515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56820.1"
                     /translation="MAMKTISDIINSKKMSRDYRCSHEFQAYGNKLSEDLGDKKHRAL
                     YIKLAKTEDRSLLEEAKDSVLSSRNATTKGRLFMWKVGQLKKERKEKQKENAENSKVE
                     KVAESKTKVGKTEKEADKSSPKN"
     gene            complement(4515..>5935)
                     /locus_tag="P5014_01520"
     CDS             complement(4515..>5935)
                     /locus_tag="P5014_01520"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HRY56821.1"
                     /translation="SKITKISTLLKETDPLSAKHITRIILGTMRLGFTELTIIAALAN
                     YLGNKSLTKEIEAKYNIHPDIGLIAKKIKDEGIKGIKTINMEIGVPILSQKAQRVSGI
                     GEIMERINKVWAEFKFDGTRVQLHFAKKGFYQKKHMNDKTGQSNLFGDEKATSLVRTF
                     TRNLEETTHQYPDIVEAAVKQIKANSVILDGEGIGYNKATGEFLPFQETMQRKRKYNI
                     KETAKNIPFKYFVFDILYLNGESVIEKPLHERRKLLDSIIGPGDVIQTDEYLETDEFE
                     KLEEYFEMAKEKGLEGIVVKTPGDPYQAGARSYSWIKYKKADEKLLSDTVDCVILGYY
                     NGRGVRSKFGIGGLLVGVYDKEKEVYKTVSKIGTGLTEEDLKYLKKKCDQIQVKNKPK
                     NVEMDKIFTPDVFVSPKMVIEIGADEISVSPTHTAGYALRFPRLIKFRDDKNPEDTTN
                     VSEIEKMYKNQRKNINGDEDNL"
BASE COUNT         1596 a         1099 c          906 g         2234 t
ORIGIN      
        1 tggttattac ttttcttgtt ttttctattt gtttatcggt gtaaattacg gtagggaaac
       61 cctctaatct ttggattgtg gggttctttc ttagactggg tagtttaatt tcgtctttta
      121 ataaaaaata tcccgcaccg aaaattaatc ccacaagtac tagtaacggt aacaaactaa
      181 ctcttaaaaa tgcatcttgt ttattttttt tcataaaaaa acctttctaa tttctaaata
      241 attgaactta atttttcttt agcctcttcg gcctcttttt tatttggtac ttttccgtgc
      301 cactgatatt tattttccat aaaatctacg ccttctccgg gtattgtgtt agctattata
      361 gcagttggac gatgatttat cgattccgct actttacagg cgcttattat ttcttcaata
      421 ttattcccgt taatttctaa aacataccaa ttgaaactct ccaacttatc cctaaggttt
      481 tcaagcggca ttatatcctc ggtatttcca tcgctttgaa tatagttcct gtcaattatc
      541 catgttaaat tatcaagttt tttattcggg gcaaacatag cggattccca gatttgacct
      601 tcattaagct caccgtcgcc ggtcatacag tatactctgt aagaagcttt attcattttt
      661 ccagctaaag ctataccgat ggcttgggag agtccctgac ctaaagatcc actggtgttt
      721 tcaattccag gtagcgagtt aatttttggg tgtccttgta attttgaatt tatttttctt
      781 aatttccaca actccgtttt tggaaagtaa cctctctcag atagagtagc ataccaaata
      841 gggcagatat gtccgtttga tagtataaac ctatcccggt cttttttatt agggttggag
      901 ggatcgattt ttaaaatatg aaaataaaga gttgaaaata tttccgcagt tcccagagac
      961 cctgcggtat gccctgtttt tgcttctgtt aacatatcta ttacaatttg acgaagcttg
     1021 tttgatattt tacgcagctc ttcaggattt ttctttatac tggtgggcat tggtaatagt
     1081 atatcacggg tatgttgagg atattagtat ttttagatgt ttttatgctt ttcctgtttt
     1141 gactttttgt cttatgtatt gtattatcac taactggtta acttggacag aaaagagccc
     1201 agaagaatca aatgcccaaa tgctcgtctt ttgcctcctt tctcgggacg ggcgctctct
     1261 cctttctaga tctatagggc tttttctttc atattagccg tagttggagt ttggttgctt
     1321 atctcgtgta gtgtgatctt aggaatatat ctacacgcaa gtaaccaagc tccatctttt
     1381 ttattatttc ttatgtttat taagggttag tttagaaatt tttctaattt aatgnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntgggct ccttctgtgg aagctctcat
     1561 aacaccggga ccacctccgt ttactattcc gtatccgttt tcagctaaaa tttttgcagt
     1621 cgcttttgct tgtatgtaag gttcgtcctc ttcgttccaa gcggcgccgc ctaaaataca
     1681 aaccatcaaa ggtcttttaa ctttttcttc tttattctcc atgggccgtt ttcctttctt
     1741 cctcaaataa atttgaaaat tcaataaact tttgttttgg attttcagta ttaaatattg
     1801 acgaaccgat gactactttg tcaacccctg tttttaccac cgaaataaaa ttatcacggc
     1861 atacaccgcc atctaacagg gtttctactc cggggaattt atttttaaaa tctattatct
     1921 tgtccattac ttcaggtaaa aactcatttc cttgttttcc cgggtttact cccataaatt
     1981 gtataacgtc tgttttgtct aaaaatggta ttagtagaga agtgtcttga tctgtattga
     2041 tggatattcc tgttttataa cctgaatttt tagcgagttt gataaattcc ataattttat
     2101 ttttatcttc tgtcagttga gtaataaatg tgcttacatt gcttatttta cctttactaa
     2161 aaataaaaga tccatttaat tttttaatat attttttagg atcatcaacc ataaggtgaa
     2221 ttatgatttc tatatccgtc ttaatgtttt taagtttatt taaatcggta aatgtttttt
     2281 cttttattaa tgtgttatcc agtacgtcta tctgtattgt tttggcaaca tctttaacaa
     2341 gatccacttt attgattata ttttttaaat ctttttctaa tattgcaggt atgatcatat
     2401 ttcttctaat tctgctattt tctgaattct tctaatgtgt ctatcctcac cagaaaaatt
     2461 ggttgaaagc catgtatgca gaatctcaag agcctcctct ttagttatgt aatctgctgg
     2521 caaaaccaat acatttgtcc cgtcgtcgtt tcttgatgag gctgcgtgtt ttggattcca
     2581 agagagagct gctctaatcc catcaagttt gttagccgcg atgcttatac ccacaccgtt
     2641 cgcacatata agtactccca tattttcaaa atcctcttga attttttctg ctacaagttt
     2701 tgcaaaatca ggataatcat cttcagggtc tgaatctttt ggtcccaaat caacaattcc
     2761 aagtcctaca gatactaaag attcaacgag atattttttt aattcaagac ctctgtggtc
     2821 agaagatata taaatcattt tttattgcct ttgtttgtaa atataaatgt taaagaaagg
     2881 attattaaaa gtatagatat agtttgagct acattcaagg taagacttgg tgataaatgt
     2941 gttgtccacg gatctattct tagaaattcg agtgagaatc ttatgacaga atatccaagt
     3001 agatatactg acataaaaaa cccgcttttt agcttattgt gttttctttt gtatgttcta
     3061 tacaaaaaaa taaaaagaat taaattaaga attgattcgt aaataaaaag cgggtggaat
     3121 ttttcattat taaaaaacat taacggtcga ttttccggat taatataaat tccccagggt
     3181 agagtagtag gtttcccgaa tatctcgtta ttaaaaaaat ttccccatct tcctgtagct
     3241 tgtcccaaag gcgtcaccac cgatattata tccagccacg ggagtatttt tttacctttt
     3301 actttaagat aaattacacc tccgcctatt cctcctaaaa cagctccaaa gatacccaaa
     3361 ccaccattcc agagaaatag tatttccaca ggatttttta tgtaaaaatc aagatagtct
     3421 aaaacgtggt ataacctggc tcctataata ccaaagataa ttgcccataa agacagcccc
     3481 cacagagtat cttcatcttt tttttcaata attccttccg cagttttaat agatataaaa
     3541 attgcgatgg atataagtat tccgtaaggg tttatcacta tacgaagtag agggctatgg
     3601 ctaaaactat aattaaacct acaatgaaaa agataacccc ctctttttta ggagttccct
     3661 ttatagccca aaaccaatcc ttcgtcctat cctcgaaaag tgcttctgta agtttgtaaa
     3721 tagagttagc gaatacagac attacaaacg cttttataaa gtatgaagta tttgtatatg
     3781 aagtaaatac aacgagaaag atcaggacaa actgaaatat tatactattc aaagatttgt
     3841 cttttacttc gtccttatgt tcattaataa aggaaattaa atcaccaaaa tctttattct
     3901 ttatataggc aaatacagtc ttagcatatt cagtgttggg ttcaaatata tatgggtaaa
     3961 gtataaattc tatgtctaaa agaaaagttc ccagcaacac acctacaaaa acctgtgcaa
     4021 tatcaagtgg ttttctcgct atactcatcg ctaaaattgt tattcctaag gtgattgaaa
     4081 ctagcagggg gattttgtaa taggttaata tttttttaat cattgtttta ataatactta
     4141 cttattgttc cgacttcaat caattttttg gcgatgattt gtcagcttct ttttctgttt
     4201 tacctacttt tgttttagat tcagccactt tttctacctt ggaattttct gcattctcct
     4261 tttgtttttc tttgcgctct tttttaagtt gcccgacttt ccacataaat aatcttcctt
     4321 tggtagtggc atttctcgaa cttaggaccg aatcttttgc ttcttctaag agacttcgat
     4381 cttcggtttt tgctaacttg atgtataaag ccctgtgttt tttatctcct aaatcttctg
     4441 atagtttatt tccataggct tgaaattcat gggaacatct gtaatccctg ctcatttttt
     4501 tgctatttat tatgtcagag attgtcttca tcgccattta tatttttcct ttgattttta
     4561 tacatttttt ctatctcaga aacgtttgtt gtatcttcag gatttttgtc gtctctgaat
     4621 tttatcaatc ttggaaatct taatgcgtag cctgcagtat gagtgggaga tacactaatt
     4681 tcatcggcgc cgatctcaat aaccattttt ggagaaacaa aaacatcggg ggtaaatatt
     4741 ttatccatct ccacattttt aggcttgttc ttcacttgaa tttgatcaca ttttttcttt
     4801 aaatatttaa gatcctcctc tgtaagcccg gttccgatct ttgatactgt tttataaact
     4861 tctttttctt tatcgtaaac acccacaagc aacccaccga ttccgaattt tgatctcaca
     4921 ccccttccgt tatagtagcc taaaatgaca caatcaacgg tatccgagag cagtttttca
     4981 tccgcttttt tatactttat ccaggaataa gatcttgctc cggcttggta gggatcgccc
     5041 ggcgttttta ctactatgcc ttccagacct ttttctttgg ccatttcaaa atactcttcc
     5101 aacttttcaa actcgtccgt ttcaagatat tcgtcggttt gaataacgtc tcccggtccg
     5161 ataatactat ccaaaagttt tcttctctca tgaaggggtt tttcaatcac agactctccg
     5221 tttaaatata aaatatcaaa aacaaagtat ttaaacggaa tatttttagc agtttcttta
     5281 atgttgtatt ttctctttct ttgcatagtt tcctggaagg ggaggaactc tccagttgct
     5341 ttgttgtatc caattccttc gccatctaaa ataaccgagt tggcttttat ctgctttact
     5401 gcagcttcca ctatatcagg atattggtgc gtagtttcct caagatttct ggtaaaggtt
     5461 ctgacaaggg aggttgcttt ttcatctcca aataaattgg attggcctgt tttatcattc
     5521 atatgttttt tctgataaaa acccttttta gcaaaatgaa gctgaactct tgttccgtcg
     5581 aacttgaatt ctgcccacac cttgttaatt ctttccataa tttcccctat cccactcacc
     5641 ctttgggctt tttgcgatag tatcgggact ccaatttcca tatttattgt ttttataccc
     5701 ttaatacctt cgtccttgat ttttttagct attaaaccta tgtcggggtg aatattgtat
     5761 ttagcttcaa tttcttttgt caaagattta tttccaagat agttggcaag agctgcaatt
     5821 attgttagtt cggtaaatcc aagcctcatt gttcctaaaa ttattcttgt gatgtgtttg
     5881 gctgacagag ggtcagtttc tttcaaaagt gttgatattt ttgttatttt actgt
//
LOCUS       DAOTFA010000028         5495 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate AMR_MDS_3115
            NODE_10665_length_5495_cov_6.090074, whole genome shotgun sequence.
ACCESSION   DAOTFA010000028 DAOTFA010000000
VERSION     DAOTFA010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868139
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (wastewater metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:36:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 536
            CDSs (total)                      :: 521
            Genes (coding)                    :: 520
            CDSs (with protein)               :: 520
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5495
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10665_length_5495_cov_6.090074"
                     /isolate="AMR_MDS_3115"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="P5014_01525"
     CDS             complement(<1..254)
                     /locus_tag="P5014_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56822.1"
                     /translation="MKHIVDNKKELLVKTEKGQTLIIAVIIMAIALAVGVTVSSRFMS
                     TLKNISESDNSTRALAVAEAAIENMLVVPQETLENYINYN"
     gene            complement(266..787)
                     /locus_tag="P5014_01530"
     CDS             complement(266..787)
                     /locus_tag="P5014_01530"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRY56823.1"
                     /translation="MKQANNQKGFTLIEMLLVVLLLALTVGLTSDMLLSLIRSNTKTQ
                     VINEIEQQANFVTLKLEKELRDSMDASTGAQNSLVITLRSNSQVTYQLNESNVLQRQV
                     GAGGFVNLTSSSSPGGVKVTCADASPCFDVTATNPKTVVTRLLFEQAQVDAGPTYRGD
                     VKIESTITIRGDY"
     gene            complement(784..1326)
                     /locus_tag="P5014_01535"
     CDS             complement(784..1326)
                     /locus_tag="P5014_01535"
                     /inference="COORDINATES: protein motif:HMM:NF019575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRY56824.1"
                     /translation="MKKQKDNGILKKKRSQKGFALVEVIAALGVSALVITALISLSIS
                     TLRTSLDSELLLEGTKIANRQVELVRAYRDGYIDADTPRSWEAFILSLQECQVFNSGP
                     LFSCYMNEDGVLVHEVGKKGTGAKQIKYQFIITNSDGSAVDTSNIPSVVRISVSVTWA
                     VGDQQKGSYIYTDLSNWRGR"
     gene            complement(1328..1861)
                     /locus_tag="P5014_01540"
     CDS             complement(1328..1861)
                     /locus_tag="P5014_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRY56825.1"
                     /translation="MNANLPNHSKGFTLIELLVVMSIIAILGGGIIPSFSRYLKDQNL
                     KQAQEQLKSDLRTIQNKALTGASSNEEINGVRARYWGVYLRSGSETVDYFISTDTSSC
                     LPIFSGTTQNGISKGNFKIPNTIVFRGSNNMCLFFDIKNGDIVSSVGNIPINIDYASP
                     NETVGKDVTFNSAGLIY"
     gene            complement(1883..2377)
                     /locus_tag="P5014_01545"
     CDS             complement(1883..2377)
                     /locus_tag="P5014_01545"
                     /inference="COORDINATES: protein motif:HMM:NF019934.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRY56826.1"
                     /translation="MNISIKNQKGFTFVELMVVIAILMAVFTIISVLVHPLDQMKKAR
                     DNQRLSDINILDRAASEYLMDNERYPDQENVLRCSNILPSGSSDLDNSNPGWIYENLS
                     SYTEKLPIDPLNTDIFRYCYIHDSTSYEISSKLEYYIEKMSEDGGNSVEFFEVGNNLN
                     LITH"
     gene            complement(2379..2909)
                     /locus_tag="P5014_01550"
     CDS             complement(2379..2909)
                     /locus_tag="P5014_01550"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRY56827.1"
                     /translation="MIKKQKGFTLVEMIIVTVIIGILAGIVITVINIPRVQARSRDSK
                     RIGDIKRIQTALELYFADNRQYPVASSSFIPAQSLAITPSYIDQIPTDPLSGNNTKNN
                     AIRCMNNIFAYGYYYLSDANGARYVITAINELPLSTEDPNVCFNVPNCDTDITGIECG
                     GSSDSSPYCYCVQNPM"
     gene            complement(3071..3733)
                     /locus_tag="P5014_01555"
     CDS             complement(3071..3733)
                     /locus_tag="P5014_01555"
                     /inference="COORDINATES: protein motif:HMM:TIGR02532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HRY56828.1"
                     /translation="MNKLIKKSFHRKEKGFTLIELLIVIVIIGILAGVLIAVINPVRQ
                     QNRSRNATIRASMLKVAFALNATKAGIGRLPNNSELTTELENLQTNAANCVTTNADLL
                     DCQFSISGVAMPKYCAENSVAPNATGSGVACWMRMLSSTNSTMTDLISGQFRVVAAAY
                     DLDTVNVSRDIFVFDSHQGMLKCRGNTTYTTTGTALVPFTIITETEYADRTTAGCVIL
                     SE"
     gene            complement(3907..4314)
                     /locus_tag="P5014_01560"
     CDS             complement(3907..4314)
                     /locus_tag="P5014_01560"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY56829.1"
                     /translation="MDEKKVLKNNILIVEDEHDIREIYAEVLNMAGYVVDQVADGEKG
                     MQKIRDDNWDILLLDIMLPGKDGIKILKEIKDHPELKKGIVIALTNLNSENIIQEVFG
                     SGADGYLIKSEITPDKVVEEVARILANQEAGRN"
     gene            complement(4462..4770)
                     /locus_tag="P5014_01565"
     CDS             complement(4462..4770)
                     /locus_tag="P5014_01565"
                     /inference="COORDINATES: protein motif:HMM:NF024061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNHCP domain-containing protein"
                     /protein_id="HRY56830.1"
                     /translation="MSGKFIKKVENFVCENCGYVVEGNGFTNHCPKCLWSKHVDVFPG
                     DRNENCGGLMRPIGLIIENNGEVIYHKCTLCGDVRACRVSKDDDRDEILKLSQIPLSS
                     "
     gene            4828..>5495
                     /locus_tag="P5014_01570"
     CDS             4828..>5495
                     /locus_tag="P5014_01570"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HRY56831.1"
                     /translation="MEKQTVQLKEEELLALVDKNRALDATYKLIEIILGTNDFDEMTQ
                     KIADIIPMALGYELAILWLIDEKKEKLVKTSISQTIKDEQLMNKLKDSFKNSSIALGY
                     QDNLCVKAITEQKQYVSAELFDFLGPAVTYDSAKEIQNMMGTDLNIATPLMVRSTPIG
                     VLIISMSKDEKGLSDFEKEMLRKFSENAGIALENSRLYKNLKDAKNDLGEAYENLIVL
                     NKMK"
BASE COUNT         1843 a         1017 c          864 g         1771 t
ORIGIN      
        1 ttattataat ttatatagtt ttctaaagtt tcctgaggaa ctactagcat attctcaata
       61 gcagcttctg ccactgctaa agctcgggtg gagttatctg attcggagat gttttttaga
      121 gtggacataa acctacttga aactgtgaca cccacagcca gtgctatcgc cattattatt
      181 acggcaataa ttagtgtttg acctttttcg gtcttaacca gaagttcttt tttattatca
      241 acaatgtgtt tcattttaaa aattatcaat aatcacctct aatggtaatg gtagattcaa
      301 tttttacgtc ccctctgtat gtaggaccag catctacctg agcttgttca aaaagtaatc
      361 ttgttaccac cgttttagga tttgtggccg ttacatcaaa acaaggagac gcgtcagcac
      421 aagttacttt tacaccacca gggcttgaac ttgaagttaa gttaacaaaa ccaccggcac
      481 ccacctgcct ttgaagtaca ttgctctcat ttagttggta agtaacctga gaatttgatc
      541 ttaaagttat taccaaacta ttttgggcac cggtactcgc atccattgaa tctcttaact
      601 ctttttctaa ttttaaagta acaaagtttg cttgctgttc tatttcattt ataacttggg
      661 tttttgtatt tgatctaatt aatgacagca acatatcgct tgtcaaacca acagtaagcg
      721 caagtaaaag taccacaagt aacatttcta ttagagtaaa gcctttttga ttatttgcct
      781 gcttcatctt cccctccaat ttgaaaggtc ggtgtaaata taagaacctt tctgctgatc
      841 tcccaccgcc catgttactg aaacggatat ccttactaca gatgggatgt ttgatgtgtc
      901 aaccgctgaa ccgtccgagt tggttataat aaactgatat ttgatttgtt tagctcctgt
      961 gcctttttta ccaacttcat gaactaatac cccatcttca ttcatatagc aagaaaataa
     1021 tggaccagaa ttaaaaacct gacactcttg taaacttaaa ataaaggcct cccaactacg
     1081 aggagtatca gcatctatat aaccatctct ataagccctg actaactcaa cttgcctgtt
     1141 agcaatttta gttccttcca gtagaagctc gctatcgaga gaggttctta aagtagatat
     1201 ggagagagat attaaagctg taataaccag tgcggatacc ccaagggcag caatgacttc
     1261 aacaagagca aaaccttttt gagatctttt cttttttaat attccattat ctttttgttt
     1321 tttcatttta ataaattaac cccgcagaat taaaggtgac gtctttacca acagtctcgt
     1381 ttggacttgc gtaatctata ttaataggga tattaccaac gctagataca atatctccat
     1441 tttttatatc aaagaaaaga cacatgttgt tgctacccct aaaaacgata gtgttgggaa
     1501 ttttaaaatt acctttggat attccatttt gggttgtgcc ggaaaaaata ggaagacaag
     1561 aagaagtatc agtagatata aagtaatcaa cggtttcaga accactgcgt aaataaacac
     1621 cccaatatct tgctctaaca ccgtttattt cttcatttga ggaagcacct gttaaagctt
     1681 tgttttgaat agttcttaaa tcacttttta attgttcctg agcctgtttt aaattttgat
     1741 cttttaaata tctcgagaaa gagggaatta taccgccacc aagaatagca ataatagaca
     1801 taactaccag aagttcaatt aaggtaaaac cttttgaatg attaggcaga ttagcattca
     1861 tattaaaata ttaacataaa aatcaatgtg tgatgagatt caaattattc ccaacttcaa
     1921 aaaattcaac actattgcct ccgtcttcag acattttttc aatataatat tccaacttag
     1981 aacttatctc gtaacttgta gaatcatgaa tataacaata acgaaaaata tcggtgttta
     2041 ggggatcgat tggtagtttt tcagtataag aagaaagatt ttcataaatc catccggggt
     2101 ttgagttatc taaatccgat gaaccggagg gcagaatatt actgcatctt aaaacattct
     2161 cttgatcagg atatctctca ttatccataa ggtattcgct ggcagcccgg tctaaaatat
     2221 ttatatccga aagcctctgg ttatctctgg cttttttcat ttgatcgagc ggatgtacaa
     2281 ggacactgat aatagtaaaa acagccatta aaatagcgat gacaaccatt aattcaacaa
     2341 aagtaaatcc tttttgattt tttatggaga tattcatgtt acattggatt ttgaacacaa
     2401 tagcaataag gactactatc agaagaacct ccgcactcta ttcctgtaat atcagtgtca
     2461 cagttgggta cgttaaaaca cacattaggg tcttcggtac taagtggcaa ttcattaata
     2521 gcagtgataa catatctggc accatttgcg tctgaaagat aataataacc atatgcaaaa
     2581 atattgttca tacaccttat agcattgttt tttgtattat taccgctaag cgggtctgtt
     2641 ggtatttgat cgatatagga tggtgtaatt gccagagatt gtgcaggaat aaaactagaa
     2701 gaagcaactg gatactgacg attatcagca aaataaagct ctagtgcagt ttgaattctt
     2761 tttatatcac ctattctttt cgaatctcta gaccttgctt gtactcttgg tatgtttatt
     2821 acagtaatta ctatacctgc aaggatacca ataataacag taactattat catttctact
     2881 aatgtaaatc ctttttgttt ttttatcatt tgttataaat ccgtaaaatt tatttacaaa
     2941 aagtttttag ataagtttga aattttcaaa cgtatttaaa agtttttaat ttttaaattt
     3001 ctaacaaatt ttttaggtcc taagccccgc cgaaggcggg gctagaacgc agaataataa
     3061 acaactatag ttattctgaa agaatcacac aaccagcagt tgtacggtca gcatattcag
     3121 tttcagtgat aatagtaaac ggaacaagag cggttccggt agttgtataa gtagtattcc
     3181 cacgacattt caacattcct tgatgtgaat caaaaacaaa tatatctctg ctcacattaa
     3241 ctgtatccaa atcataagca gctgcgacaa ctctaaattg ccctgaaatc aagtctgtca
     3301 tagtagagtt agttgaagaa agcattctca tccaacaagc tactccagaa cctgtagcat
     3361 ttggggctac agagttttca gcgcagtatt ttggcatggc gacaccacta atactaaatt
     3421 ggcaatcaag taaatcagcg tttgttgtaa cacaattagc agcattagtt tgaagattct
     3481 ccaattcggt ggtaagttct gaattattag gaagtcttcc aataccagcc ttagtagcat
     3541 tgagtgcaaa ggctactttt aacatagacg ctcttatcgt ggcatttcta gacctatttt
     3601 gttgtctaac tgggtttata acagcaataa gaacacctgc taaaatacca atgatgacaa
     3661 taacgatcaa aagctcgata agtgtgaaac ctttttcttt tcggtgaaac gactttttaa
     3721 ttaacttatt cataatttcg tttcaccccc cttccaaaac ctaatttttt atttttttca
     3781 attatcatag caacaaggca gattatttta gttgcctatc taaagtaaag tatggactga
     3841 attattttta gtgtcaagaa ataaaaggtt atcaataatc agagaaagat tcgaatcaaa
     3901 aataatttaa tttctaccgg cttcttgatt agctaaaatt cttgcaacct cttcaacaac
     3961 cttatcaggt gtgatttctg attttattag atagccatca gcacccgaac caaaaacttc
     4021 ctgaataatg ttttcgctat ttaaattagt aagagcaata acaatacctt ttttcaactc
     4081 ggggtggtcc ttaatttctt taagaatttt tataccatcc ttaccgggaa gcattatatc
     4141 cagaagtaag atatcccaat tatcatctct gattttttgc atacctttct cgccgtcagc
     4201 tacctgatca acgacgtagc cggccatatt taaaacttca gcatatattt ctctaatatc
     4261 atgctcgtcc tctacaatga gaatattatt ttttaatact ttcttttcat ccatacttaa
     4321 aatttaccac taataaaaaa atgcttcaag ggaatataaa ataatttcta aaaagtacct
     4381 ataccaaaag agtagagaag aggttgaaaa atataaatgc caaaaaatct aaattaaagt
     4441 aagcgatcaa attaaaaaaa tctaactcga aagaggtatc tgacttaatt tcaaaatctc
     4501 atccctgtcg tcatcttttg aaaccctgca agctctaaca tctccgcaca aagtacactt
     4561 gtggtaaatt acttcaccgt tattttcaat tattaatccg atgggtctca tcaaaccgcc
     4621 acagttttca tttcgatcac caggaaatac atcaacgtgt tttgaccaca aacactttgg
     4681 acaatgatta gtgaatccat ttccttcaac cacataacca caattttcac aaacaaaatt
     4741 ttcaactttt ttaataaatt ttcctgacat agcttttaat tatttaaaaa ataccatatt
     4801 attatataca gtaattttga attaataatg gaaaaacaaa ctgtccaatt aaaagaggag
     4861 gaactgctgg ctctggtaga taaaaaccgg gctctagacg cgacctataa actaattgaa
     4921 ataatcctgg gaacaaatga ttttgacgag atgactcaaa aaatagccga catcattcca
     4981 atggccttgg gttatgaact cgcaattctt tggctaatag atgaaaaaaa agaaaagctg
     5041 gttaaaacat caatatctca aactataaaa gacgagcagt taatgaacaa acttaaagat
     5101 tccttcaaaa attccagcat cgccttaggc tatcaagata atctctgtgt taaagctata
     5161 accgaacaaa aacagtatgt ttcagccgaa ctttttgatt ttttaggacc cgcagttaca
     5221 tatgactccg caaaagaaat tcaaaatatg atgggaacag acctaaatat cgcaacgccc
     5281 cttatggtta gaagtactcc tataggagtt cttattataa gtatgtctaa ggacgaaaag
     5341 ggccttagcg attttgaaaa agaaatgctc aggaaatttt ctgaaaacgc gggaattgct
     5401 cttgaaaatt caaggcttta taaaaattta aaggatgcaa aaaacgattt gggcgaggct
     5461 tatgaaaatt taatagtact aaataaaatg aagga
//
LOCUS       DAOTFA010000029         5292 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate AMR_MDS_3115
            NODE_11242_length_5292_cov_5.994081, whole genome shotgun sequence.
ACCESSION   DAOTFA010000029 DAOTFA010000000
VERSION     DAOTFA010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868139
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (wastewater metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5292)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5292)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:36:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 536
            CDSs (total)                      :: 521
            Genes (coding)                    :: 520
            CDSs (with protein)               :: 520
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5292
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11242_length_5292_cov_5.994081"
                     /isolate="AMR_MDS_3115"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1975
                     /locus_tag="P5014_01575"
     CDS             <1..1975
                     /locus_tag="P5014_01575"
                     /inference="COORDINATES: protein motif:HMM:TIGR01494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HRY56832.1"
                     /translation="PINKNVDEKVFMGTTVSSGQAVMLIESIGATTEVGKIADKIQDK
                     DDVTPFQMQLKRFSNHLLIIIAGIIVFVFIVGVLRGMDITEIFITSVALAVSSIPEGL
                     LVSLTIVLTIGMQKILKRRGLVRKLAAAETLGGVSVICVDKTGTLTLGQTEVSDFKGN
                     KEALAIQLSVANDLDDPVLISGYKWAKNIYSNDESSYTRIDSIPFTSKERFFISLNEL
                     DSDNNIIFVNGAPELLLSWSTLNQSEKDTVTQEIEMLTNEGKRIIGFGRKIVDKNKKT
                     LEVEDAKCDLEWVGIVAFSDPVRDGLIEPLRETYEAGIKTVVITGDYPKTSIYVLSQL
                     GINVSDDEYITGDILSNMSDEELKNRIEKIKLFARTTPDQKLRIVNAYRDNGEVVAMI
                     GDGVNDAPALHRSDIGIVVNEASDVARESADLVLLDSSFSTIVHAIEEGRVMFENIRK
                     IVLYLLCDAFEEILVVLAGIIFGLPLPVTAVQILWVNLVSDGLPNLALTIDPKRRGIM
                     KDKPRSPHEHIITSWMINLIMFVSFVAGITAFLSFYLVYKNTGSITLARSVTFVTIGI
                     NSLVYVFSVRAPFTSFIKSRMFKNKWLGLSVLAGFILQFLPFATENFRNFFRVEKLSA
                     TYWGIAFGLSVLMFLIVEMFKHVFHANIVKGHE"
     gene            complement(1979..>2539)
                     /locus_tag="P5014_01580"
     CDS             complement(1979..>2539)
                     /locus_tag="P5014_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56833.1"
                     /translation="TRISSKSKRYLILLSALFIIPTLFVVLKSYSVAKNDDFGYSRAV
                     SKTRYHIYKPAVMPLGLVYTSNFITGKEMAGLKDGVQVVYDYTLEEVVDMKKSRPVIV
                     KQVGVHRSFNINEYVAKESGMYSQTSTVNIPNTVGGSGYLILNPLGSSGDNLNLVFQK
                     TDNVLISISTTDSGKQDLIDIAVSLE"
     assembly_gap    2540..2639
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2640..3085)
                     /locus_tag="P5014_01585"
     CDS             complement(<2640..3085)
                     /locus_tag="P5014_01585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56834.1"
                     /translation="MVTQELIEYIKYQKSKKVADEDIVRELIENNWVRGDIENALNKV
                     NSLDSSTPFETQEKDRDKVFVVDSTISHPATVVKENVEIPIGDKASVIIGIISMLFIV
                     ISFLFIYKAGMMVAVMSIIDHFSYTEGSMYYFIKQFPMYGWVVISFA"
     gene            3181..3402
                     /locus_tag="P5014_01590"
     CDS             3181..3402
                     /locus_tag="P5014_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004450172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HRY56835.1"
                     /translation="MKIEVCVHANSKSPRIDKTSDGTLHIYVREPAIEGKANRAVVEV
                     LSELYKTPKSSINLIHGIKSKKKIFEINK"
     gene            complement(3404..4813)
                     /locus_tag="P5014_01595"
     CDS             complement(3404..4813)
                     /locus_tag="P5014_01595"
                     /inference="COORDINATES: protein motif:HMM:NF014877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HRY56836.1"
                     /translation="MDKQILDTIFRAYDIRGRYPSELNSQFYENLGKAYVTFFKPTKV
                     AVGYDIRPESIEMKDSLAKGLCEMGCDVVDIGEVPTEALYFATGEYPETFDGGLVVTA
                     SHNGAGWNGCKMVTKNAKPISGTTGLMDLKKIILENRYEKVRDVKGAVSTMDVMPEYK
                     KKVLSFITSENKKPLKIVVDAGNGIGGKVFDYIFCDLENEFLNVERMYFDPDGTYPNH
                     VPNPMEEENVEEIKNRVVEKDADFGIAIDGDADRAFFIDKKGRRPDGVYVGVLLAKSI
                     LSHDPDNKKIVHDPRITWPFIKEAQKLGAEVHQSMAGHSFFKAAMAEKQALFGAEASA
                     HFYYRDFYNCDSGMVTIAQMLNMYFDGFELTQAVDYLFENYPNSGEVNYRVENAIGLI
                     NQIEEFYKNQGAKIEKIDGLSVEFEDWRFNLRTSNTQPLLRINLEAKTKNLVIEKFLE
                     VENMIGAVRDNIPALEELR"
     gene            complement(4825..>5292)
                     /locus_tag="P5014_01600"
     CDS             complement(4825..>5292)
                     /locus_tag="P5014_01600"
                     /inference="COORDINATES: protein motif:HMM:NF028476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NdvB"
                     /protein_id="HRY56837.1"
                     /translation="HGAVWAMKAYAMMKNGDKAYELFNTLNPINRTLDRDSVNIYKSE
                     PYVMAGDIYSNPQHLARGGWSWYTGSAGWLYRVAIEDILGFKVKQDYFTLEPVLPKEW
                     TEYEMIYSHNNTLYSIKVERGEKKGLYINGNKTDDSKIYFRSDMGNIDIYLVI"
BASE COUNT         1682 a          945 c          845 g         1720 t
ORIGIN      
        1 acccattaac aaaaatgttg atgaaaaggt ttttatgggg actacagtat cgtcaggtca
       61 ggctgttatg cttattgaaa gtatcggagc caccacagaa gttgggaaga tcgctgataa
      121 gattcaagat aaggacgatg ttacaccttt tcaaatgcaa ctcaaaaggt ttagcaatca
      181 cctattaata ataatcgccg gtattatagt ttttgttttt atagtaggcg ttttacgtgg
      241 aatggatata acagaaatat ttatcacgtc tgtagcgtta gctgtttcca gtattcccga
      301 aggtttgtta gtatctttaa ctattgttct aaccatagga atgcaaaaaa tattaaaaag
      361 aagggggttg gttagaaaat tggctgcagc agaaacattg ggaggagttt cagtaatctg
      421 tgtagataaa acggggacac tcactcttgg acaaacagaa gtctcggatt tcaaaggaaa
      481 caaggaagcc ttagcaattc agctttccgt tgctaacgat ttagacgatc ctgttttaat
      541 atcaggttat aaatgggcaa aaaacatcta cagtaatgac gaatccagct atacaagaat
      601 agacagcatt cctttcacct caaaggaaag gttttttata tctttaaacg agttagatag
      661 tgataacaat ataatatttg taaacggagc cccggaatta cttcttagct ggagtacatt
      721 aaatcaatct gaaaaagaca cagttacaca agaaattgaa atgcttacaa acgaaggtaa
      781 gagaattatt ggttttggaa gaaaaatagt tgataaaaat aaaaaaacat tagaggtaga
      841 ggatgcaaag tgtgatttag aatgggtagg aatagtagct ttttcagatc ctgtcagaga
      901 tggactcatc gaacctttaa gagagactta cgaagctggt attaaaactg tggtaattac
      961 aggagattat cctaaaactt caatatatgt actttcacag cttggaatca atgtttccga
     1021 tgatgagtac attaccggag atattctttc taatatgtct gacgaagaac tgaaaaatag
     1081 aattgaaaaa ataaaacttt ttgcacgaac tacacctgat caaaagctta gaattgtgaa
     1141 tgcatataga gataacgggg aagtggtcgc catgataggt gatggtgtaa acgacgctcc
     1201 agctttacat aggtctgaca taggaattgt agtaaacgaa gcctctgacg tagcaagaga
     1261 atccgcagat ctagttttgc ttgattcaag tttttctaca attgttcacg ctatagaaga
     1321 aggtagggtt atgtttgaaa atatcagaaa aattgttttg tatttgttat gcgatgcatt
     1381 tgaggaaatt ttggtagtac tagctggaat tatttttggt ctacctttac ctgttactgc
     1441 tgttcagata ttgtgggtta atcttgtttc agatggttta ccaaatcttg cattaacgat
     1501 tgaccctaaa cgaagaggta ttatgaaaga caaaccacgt tccccacatg aacatattat
     1561 aacctcatgg atgataaatt taattatgtt tgttagtttc gttgctggga ttacagcttt
     1621 tctatctttc tatcttgttt ataaaaatac gggtagcatt acacttgcaa ggtcggtgac
     1681 atttgtcacc attggaatca attcattagt atatgtgttt tcagtaagag ccccttttac
     1741 atcttttatt aaatcaagaa tgttcaaaaa taagtggctg gggttgtcag ttttggcagg
     1801 atttatactt caatttttac cctttgcaac tgagaacttt agaaactttt ttagagtaga
     1861 aaaactctct gcaacatatt ggggtatagc ttttggatta tctgttttaa tgttcttgat
     1921 tgttgaaatg tttaaacacg tttttcacgc aaacattgtt aaaggtcacg aatagttttt
     1981 attctaaaga tactgctata tcaattaaat cttgtttacc tgagtcagtt gttgaaattg
     2041 agattaatac attatccgtt ttttgaaaaa caagatttaa attatctcca ctcgatccaa
     2101 gtgggtttag aattagataa cctgaaccac ctactgtatt aggaatatta actgtactgg
     2161 tttgagaata cattccggat tcttttgcca catattcatt gatattaaat gatctgtgaa
     2221 caccaacttg tttgacaata acaggtcttg attttttcat atccactacc tcttccagtg
     2281 tatagtcata tacaacttgt acaccatctt ttaacccagc catttccttg ccggtaataa
     2341 aattagatgt gtaaacaagc ccaagcggca taacagcagg tttatatata tgataccgtg
     2401 tctttgatac tgctctagaa taaccaaagt catcgttttt agctacagaa taacttttta
     2461 acactacaaa caatgtggga attataaaaa gtgcggacag taatattaag taacgtttac
     2521 tttttgacga tattctagtn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnng
     2641 caaacgaaat aacaacccat ccatacatcg gaaattgttt tataaaataa tacatggatc
     2701 cttcggtgta agaaaaatga tcaataatac tcattaccgc caccatcatg ccggccttat
     2761 agataaacaa aaaagaaata acgataaaga gcatcgaaat tatacctatg attacactcg
     2821 ctttatcacc tataggtatc tcaacatttt cctttaccac agttgctggg tgagaaattg
     2881 tactatcaac cacaaaaacc ttatccctat ctttttcttg agtttcaaat ggggtacttg
     2941 aatccaagct gtttacttta ttaagagcgt tttctatatc tccccttacc caattatttt
     3001 caattaattc acgcacaata tcttcgtctg ctactttttt tgatttttgg tactttatat
     3061 attcaatgag ttcttgtgta accatcctat ttaattaaaa caaaagattt atgatatgtc
     3121 caagaatttt tatatactta tatagtaaaa aaataaattt tagaaaattt aaaaatataa
     3181 atgaaaattg aggtttgcgt acacgctaat tctaaatcac caagaataga taaaacttcg
     3241 gacggcactt tacatatcta tgtcagagaa ccggccatcg aaggaaaggc gaacagggca
     3301 gtggttgaag tactttcaga attatataaa acaccaaaat cttcaataaa tctgattcac
     3361 ggaataaaat caaagaaaaa gatattcgag ataaacaaat aagctatctt aattcttcaa
     3421 gtgccggaat attatctctc acagctccga tcatattttc aacttctaag aatttttcta
     3481 tcaccaaatt cttcgtttta gcttctaagt ttattcttag aagaggttga gtattagaag
     3541 ttcgtaaatt aaacctccaa tcttcaaatt cgacggaaag accatcaatt ttttcaattt
     3601 ttgctccttg atttttgtaa aactcctcaa tttgattgat caaacctatc gcgttttcaa
     3661 ccctgtaatt cacctcacct gaatttgggt aattttcaaa taaataatcg actgcttgag
     3721 tcagttcaaa accgtcaaag tacatattta gcatctgagc aattgttacc atacctgaat
     3781 cacaattata aaaatcgcgg taatagaagt gtgcgcttgc ttcagctcca aacaaagctt
     3841 gtttctctgc catagctgct ttaaaaaaag agtgcccggc catactttgg tgaacttcag
     3901 cacccagctt ttgagcctcc tttataaacg gccatgtaat tcttgggtcg tgaacaattt
     3961 ttttattatc tggatcgtgg cttaaaatgc tttttgctaa caatacacct acgtaaacac
     4021 catcgggtct tctgcctttt ttatcaataa aaaacgctct atctgcatcg ccatcgattg
     4081 ctataccaaa atctgcatct ttttctacaa ctctgttttt aatttcttct acattttctt
     4141 cttccattgg attaggtacg tggttggggt atgtaccgtc gggatcaaaa tacatacgtt
     4201 caacatttaa aaattcattt tctaaatcac aaaaaatgta atcaaaaacc tttccgccaa
     4261 tcccatttcc tgcgtcaacg acaatcttta atggtttttt attttcagag gttataaaag
     4321 ataaaacctt tttcttatat tccggcatta catccatggt agaaacagcg ccttttacat
     4381 ctctcacttt ttcatatcta ttttcaagaa ttatcttctt tagatccatt aaaccggttg
     4441 taccactaat tggtttggcg tttttagtta ccattttgca accgttccaa cctgcaccgt
     4501 tgtgtgaggc agtgacaact aaaccaccat cgaaagtttc tggatactcg cctgtagcaa
     4561 aatacaatgc ttctgttggt acttcaccta tgtcaacaac atcacaaccc atttcacata
     4621 aacctttagc aagtgaatct ttcatttcaa tactttcagg tctgatatcg taacccacag
     4681 caaccttagt gggtttaaaa aaagtgacat aagccttacc taaattttca taaaactgtg
     4741 agttaagttc agaaggatac cttcctctaa tatcataagc acgaaaaata gtatctaata
     4801 tttgtttatc catcttagaa cctcctaaat taccaaataa atatcaatgt ttcccatatc
     4861 acttctaaaa tagatctttg aatcatccgt tttgtttccg tttatataaa gacctttctt
     4921 ctcacctctt tcgaccttta tactatagag tgtattgttg tgagagtaga tcatttcata
     4981 ttcggtccat tcttttggta aaactggctc taatgtaaaa tagtcctgct taaccttaaa
     5041 acctaaaata tcctcgattg cgacccgata caaccaacct gcagatcctg tataccaact
     5101 ccagccacct ctagctaaat gttgcgggtt agaataaata tctccagcca taacatacgg
     5161 ctcggattta taaatattta cgctatctct atcaagagtt ctatttatag gattcaatgt
     5221 attaaacaac tcataggctt tatcgccatt tttcatcata gcgtaagctt tcattgccca
     5281 gacagctccg tg
//
LOCUS       DAOTFA010000030         5164 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate AMR_MDS_3115
            NODE_11632_length_5164_cov_9.102564, whole genome shotgun sequence.
ACCESSION   DAOTFA010000030 DAOTFA010000000
VERSION     DAOTFA010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868139
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (wastewater metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5164)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5164)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:36:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 536
            CDSs (total)                      :: 521
            Genes (coding)                    :: 520
            CDSs (with protein)               :: 520
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5164
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11632_length_5164_cov_9.102564"
                     /isolate="AMR_MDS_3115"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            43..1587
                     /locus_tag="P5014_01605"
     CDS             43..1587
                     /locus_tag="P5014_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56838.1"
                     /translation="MIKIQLQQTDNLLSVINKIKETADLNIELEIPEESLLLENILNL
                     RLIQQQADKIEKSVTFTTSDEYGNVLIDILSGKDIELIPEEFADSAEYFNKKGFSLPK
                     IKNPFRNFKLPGFIKIKRGIILIPLIIASIFGAFVYYGNTAPKAKVVITVGAQPYTRS
                     ITIKVKSDGQTSNENMILKGSVLTTSVQETSEIDTTGVKTVGEKAEGEITLYNKTSSK
                     IELEKGDTVKYTGKSEDLPYTLKDDVEIPESTPQDPLDPASPMNPGEAKVKIVAKDIG
                     SGYNLDEGKSFEIEDYEKSELAGKNKEKISGGSSKEVKTVTQADMDKLSNQLKEISIK
                     KAEESIRGKLGTSQKLIEGSVKISVSSENFSAKVDEEKEKLSLTQGATGEALVYNENE
                     LNKFIDGYFKNVIPEGHYMPDKDKKITVNILGQSASSVLNSTEADIQVTLSSIIIPDI
                     KEDDVKENLKGKTNEESKQYIDSLKNIESYEFNISPVVPFFSKVPRDTKRIDVTLNKM
                     YKGEAL"
     gene            1584..2021
                     /gene="ruvX"
                     /locus_tag="P5014_01610"
     CDS             1584..2021
                     /gene="ruvX"
                     /locus_tag="P5014_01610"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HRY56839.1"
                     /translation="MKTNQDSALPRFLGIDYGEKNIGLALGYNGLTMPLKTVSNKTDR
                     DAIFEINKVIIENKVGNIIIGIPLTAENKETKESLIVRRFAKNLKTISKRPVIFQNEF
                     GTSKEALKEAISMDVPKKRRASNDHLAACLILKTFFEENSSSE"
     gene            2081..2266
                     /locus_tag="P5014_01615"
     CDS             2081..2266
                     /locus_tag="P5014_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56840.1"
                     /translation="MEVFKTITTIYLILGFIYAVFIATKKIDAWYWFPVNWILGPLTV
                     VYIFYLTVRGKRLPTDW"
     gene            2269..3021
                     /locus_tag="P5014_01620"
     CDS             2269..3021
                     /locus_tag="P5014_01620"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HRY56841.1"
                     /translation="MYKILFEGKKAVIFNLDDAVITGLNEIKIQVFQKVMNDVFLGYI
                     DPTSYCIPGYSFRTIWESILYANEIEKGKFKIKDLIEKTTNAYIETINNEGIIIDTTD
                     GFWQLFGELKEDHGFKTALVSNYPKPVVDKLTEKIELEGIFDVVVCNENTKEELHRIY
                     KKVLKKLKMNPKKVVSFEGSIPGVRAANKAKVDVFVIWDLKTRKSFFGDKVKEFSLDF
                     TAYPGNIDKTYEEYLAESVKGAIEDKKQRQSL"
     gene            3145..4539
                     /gene="dnaA"
                     /locus_tag="P5014_01625"
     CDS             3145..4539
                     /gene="dnaA"
                     /locus_tag="P5014_01625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HRY56842.1"
                     /translation="MNEALNNNPKEFWKAVLGQIQLEISPMVYKTMVSRTTGVSFEND
                     TLEVFCEDNFVKKNLETKFSSVVDNSVKNIGGKGIKIKYSVSKEKEVKENKDELGPLF
                     SQQKTAEKLNEEKRIKSNLNPKFSFDNFILGKNNNLAYAIATAISEKPGELYNPVFIY
                     SKVGLGKTHLIQAVGNKIIETKPGMRVVYTTGEAFTNELIETIQSGKGRGKYTANQFR
                     EKFRKADVLLIDDVQFIIGRESTQEEFFHTFNALYMSGKQILITSDRPPKDFNSLEER
                     ITSRFSSGIVADIQNPDVETRVAILRSKRDAEKENIPNEVLNFIAERVDSNIRELEGA
                     YIQVITYAKATGDVLTVDTAARALGATVKEKVVKNVNVNEILKAVCLYYSVKIQDIKG
                     KRRHKELVLPRQVAMYLMKEITDIPFMSIGELLGGRDHTTVMHGVNKIQEEIAETGKV
                     TQDIVNVKQIIFND"
     gene            4911..>5164
                     /locus_tag="P5014_01630"
     CDS             4911..>5164
                     /locus_tag="P5014_01630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56843.1"
                     /translation="MQYKERLLGLSKKIFFILIFLIPINLGKHFEIFSSYVGGVLVDY
                     LVPTIFVQDILILLVVLFWLFSGGFKKLFSTKIELFEKKE"
BASE COUNT         2035 a          790 c          942 g         1397 t
ORIGIN      
        1 gcctcaacgt caataattta taaagaaata ataggaggat aaatgattaa aatccaactt
       61 cagcaaacag ataacttact ttccgttata aacaaaataa aagaaacggc agacctaaat
      121 atagagctgg aaatacctga ggagtcattg ttattagaaa acatcttaaa tttgagacta
      181 attcaacaac aagcggataa aattgaaaaa agtgtgactt tcacaaccag tgacgagtac
      241 ggaaatgttt taatcgacat tctttccgga aaagatatag agcttattcc cgaagaattt
      301 gctgattctg ctgaatattt caacaaaaaa ggtttctcat tacctaaaat taaaaaccca
      361 ttccgaaatt ttaaactacc cggatttatt aagataaaga ggggcataat cctaatacca
      421 ttgataatcg cctcaatatt tggtgccttt gtttattatg gaaacacggc tccaaaagct
      481 aaagtggtaa ttacagtagg agcccagcct tacacaagga gcatcacaat caaagtaaaa
      541 tcggatgggc aaacaagcaa tgagaacatg atattaaaag gctcagtact aactacaagc
      601 gttcaggaga catcagaaat tgatactaca ggggttaaga ctgtaggtga aaaggcagag
      661 ggggagataa ccttgtataa taaaacttcc tcaaaaatag aacttgaaaa aggagacact
      721 gttaaataca cggggaaatc agaagatctt ccttatactt taaaggacga tgtggaaata
      781 cctgaaagta cacctcaaga cccgctggat ccagcaagtc ctatgaaccc tggagaagct
      841 aaggttaaaa tagtagcaaa agatattggc agtggttaca acctggacga aggtaaatca
      901 tttgaaatcg aagattatga gaaaagcgaa ctagctggta aaaataaaga aaaaatatcg
      961 gggggaagca gtaaggaagt taagacagtt acacaggctg atatggataa gctgtcaaat
     1021 caattaaaag agatctccat aaaaaaggct gaagaaagca tccgtggaaa gctgggaacc
     1081 tcacaaaaat taatagaggg ttccgttaaa atctctgtaa gttcagaaaa cttcagtgct
     1141 aaagtcgatg aagaaaaaga aaaattatcg ctcacacaag gagcaacagg agaggcatta
     1201 gtttataacg agaacgagct aaataagttc attgacggtt attttaagaa cgtaatcccc
     1261 gaaggtcatt atatgcccga caaagataag aaaatcacag ttaacatact gggacaatcg
     1321 gcaagcagtg tgcttaactc aactgaagcc gacatccaag tgacactaag ctccattata
     1381 atacctgaca taaaagagga tgatgttaag gaaaacctaa aggggaaaac caatgaagaa
     1441 tcaaagcaat atatagacag tttaaaaaat attgagtcct atgaatttaa catatcccct
     1501 gtagtgcctt tcttttcaaa agttccgaga gacaccaaaa gaatagatgt gacattaaat
     1561 aaaatgtata agggagaggc cttatgaaaa ccaaccaaga ctctgcccta ccccgattcc
     1621 tcggaataga ttatggagaa aaaaacattg gtttagcatt aggatacaac gggctcacaa
     1681 tgcctttaaa aactgtttct aataaaactg acagagatgc aatatttgaa ataaataaag
     1741 taattatcga aaacaaagtt ggaaacatta ttattggaat ccccctcact gccgagaata
     1801 aagagaccaa agaatcatta attgtaagga ggtttgctaa aaatttaaaa acaatatcta
     1861 aaagaccggt aatatttcaa aatgaattcg gaacatcaaa agaggcctta aaggaagcaa
     1921 tatctatgga cgtacccaaa aaaagaaggg cttcaaacga ccaccttgcg gcttgcttaa
     1981 tattaaaaac gttttttgaa gaaaactcta gtagtgagta aaaatcagat caaaaataag
     2041 ggttgtggaa ttagtccaaa attaaatata gaataatttt atggaggttt ttaaaactat
     2101 aacaacaatc tatttaatcc ttggatttat ttacgcagtt tttatagcca ctaaaaaaat
     2161 agatgcatgg tattggtttc ctgtaaactg gattttaggt ccgctgactg tagtgtatat
     2221 cttttattta acagtaagag gaaagaggct tccaactgat tggtaaatat gtacaaaata
     2281 ttattcgaag gaaaaaaagc agtcatattt aatcttgatg acgctgtaat tacaggacta
     2341 aatgaaatta aaattcaggt ttttcaaaag gttatgaacg atgtgtttct tggctatatt
     2401 gacccaactt cttactgtat tcctgggtat tctttcagaa ctatctggga atctattttg
     2461 tacgcaaatg aaatagaaaa gggaaagttt aaaattaaag acctaataga aaaaactaca
     2521 aatgcttaca tagaaactat taacaatgaa ggtattatta tcgacactac ggatggtttc
     2581 tggcaacttt ttggagaatt aaaggaagat catgggttta aaacggcgct tgtttcaaat
     2641 tacccaaagc ccgttgtaga taaactaaca gaaaagattg aattagaagg tatttttgac
     2701 gtggtcgttt gtaatgaaaa taccaaagag gagctacata gaatttacaa aaaagtgcta
     2761 aaaaaattaa agatgaatcc taaaaaagtg gtatcttttg aagggtcgat tcccggcgtc
     2821 cgcgccgcta acaaagcaaa agtagacgtt tttgtaatat gggatttgaa aacaagaaaa
     2881 tcattttttg gagacaaggt taaagaattt tcattagatt ttacagccta ccccggaaat
     2941 atagataaaa cgtatgagga gtatttagct gaatctgtaa aaggtgctat cgaagataaa
     3001 aagcaaagac aaagtcttta acaaccactc cctataatcc acattgcttg ataattgtgg
     3061 ataacttgtg taacttgtac ttctactgca aaatataaac tgcagatgct gtcccccatt
     3121 aaaagaaggg tgtttattat ttaaatgaat gaggcgctaa ataacaaccc aaaagagttc
     3181 tggaaggctg ttctcggaca gatacaactt gagatatccc ctatggttta caaaacaatg
     3241 gtttcaagaa ctacaggggt gtcttttgaa aatgacacat tagaagtgtt ttgcgaagat
     3301 aactttgtta agaaaaatct cgaaactaaa ttttcaagtg tggtagataa ctcagtaaaa
     3361 aacatcgggg ggaaaggcat aaaaattaaa tactctgttt caaaagaaaa agaagtaaag
     3421 gaaaacaaag acgagttagg gccgcttttt tcccaacaaa aaactgcaga aaaattaaac
     3481 gaagaaaaaa gaattaaatc aaacctcaat ccaaaatttt catttgataa ttttatatta
     3541 ggaaaaaaca acaaccttgc ttacgcaata gccacggcaa tatctgaaaa accgggagaa
     3601 ctatacaatc ccgtctttat ttattccaaa gtagggcttg gaaaaaccca cttaattcaa
     3661 gctgtcggaa ataaaataat agaaacaaaa ccggggatga gagttgtata caccacagga
     3721 gaagctttca ccaatgaatt aattgaaaca attcagagtg gaaaaggaag aggaaaatac
     3781 acagcaaacc aattcagaga aaaatttaga aaagcagacg tacttctaat tgatgacgtg
     3841 cagtttatta tcggaaggga atcaacccag gaagagtttt ttcatacctt taatgctttg
     3901 tatatgagcg gtaaacaaat tttaataaca tccgaccgtc ctccaaaaga ttttaacagc
     3961 ctggaagaga gaattacctc aagattcagc agcgggatag tagcagatat tcaaaaccct
     4021 gatgtcgaaa caagggtagc aattttaaga tcaaaaagag atgctgaaaa agaaaacata
     4081 ccaaacgagg tattaaattt tattgctgaa agagtggatt ctaacattag agaattagag
     4141 ggagcgtata tacaagttat aacctacgca aaggccacag gagacgtttt gacagtagat
     4201 acagccgcaa gggcgctggg agccacggta aaagaaaagg ttgttaaaaa cgtaaatgtg
     4261 aatgaaatac tgaaagctgt atgtttatac tattcagtaa aaattcagga tattaaaggt
     4321 aaaagaagac ataaagaatt agtgctacca agacaagtag ctatgtacct tatgaaagaa
     4381 attactgata tcccttttat gtctattggc gagcttttag gaggaagaga tcatacaaca
     4441 gtaatgcacg gtgtaaacaa aatacaggaa gaaatcgcgg aaactggtaa ggttactcaa
     4501 gatattgtga atgtaaaaca gataattttt aacgattaag gatttgtgga aaaagaaaga
     4561 atctaaaact aatgtggata agtaaccact tttatccaca aagtaatcca cattttttaa
     4621 gcacttttca acaaacaaca atcctttaaa tagaacatat ttaataatat gactctttat
     4681 aacttggttt aaaagcaaat gtaaatatcc acattattaa aatggttatt cgttaccgtt
     4741 caagcatata aagacttttc aacatatcca caccaactac tacaactacc tcttatttta
     4801 tatacaaata ttatattgat taaacttggg gataacctca aacgatatca ataaattagg
     4861 ttataataaa ttcattaaat ataaatatta aatctgccca atattttcta atgcaatata
     4921 aagaaagatt attgggacta tctaaaaaaa tattctttat tctgattttt ctaattccta
     4981 tcaatttggg aaaacatttt gaaatatttt cctcgtatgt aggaggtgtt cttgtagatt
     5041 atcttgttcc aactatattt gttcaggata tattaatatt gttagttgtt ttgttttggt
     5101 tatttagtgg aggatttaaa aaattatttt caaccaaaat agaacttttt gaaaaaaaag
     5161 aaat
//
LOCUS       DAOTFA010000031         5063 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate AMR_MDS_3115
            NODE_11935_length_5063_cov_6.113419, whole genome shotgun sequence.
ACCESSION   DAOTFA010000031 DAOTFA010000000
VERSION     DAOTFA010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868139
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (wastewater metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5063)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5063)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:36:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 536
            CDSs (total)                      :: 521
            Genes (coding)                    :: 520
            CDSs (with protein)               :: 520
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5063
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11935_length_5063_cov_6.113419"
                     /isolate="AMR_MDS_3115"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            187..1197
                     /locus_tag="P5014_01635"
     CDS             187..1197
                     /locus_tag="P5014_01635"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRY56844.1"
                     /translation="MKLISKKTLIFLVIILIVGFGLFFRSRKKDTKEYYTVQRQNMEL
                     IVETSGYVQSNDKADLYFQVAETITDVLVVEGDAVEKGDILATVSGQSLAQSSQVARD
                     NRDIAIFEKDLYVEKYATDTDAVGGDNEYDRQLRIYDENISKAEALYDQSLINSRKSV
                     IVAPFNGTISKVYVNKGDLSSVSRPAMEIQDVSKPVLYTNISEVDISYTDTSDKVEII
                     FDAFPDKVFTGYIYSINPAPETIQGVSYYKAEIKADNLPEKLRVGMSADINILAEKKE
                     GVLYLPLYLMDNVTADTGLVKLDGNNGSEETEIKIGLNNYKGVEVISGLNEGDVVVYD
                     RE"
     gene            1273..1953
                     /locus_tag="P5014_01640"
     CDS             1273..1953
                     /locus_tag="P5014_01640"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HRY56845.1"
                     /translation="MNLEKLNISKNSKVLFIYPHPDDETYFNAGLIQNLVKENITTRI
                     LCLTKGEASSLKYGIKHNNLGEIRKKEFESVMKFLKVSEHKILDFEDGNLGNENSLSE
                     ILEKEINLFEPDVVITFEPSGVYGHRDHIVVSQVVTDLKKLRKFNLIYSTVPFNYHTS
                     QKNSLNFKIDSKPLEPDMILKLSPNEVVNKLICLIKYKSQVNNERLIQIILKPYLYKE
                     CYCIYPNL"
     assembly_gap    2012..2111
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <2112..2483
                     /locus_tag="P5014_01645"
     CDS             <2112..2483
                     /locus_tag="P5014_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56846.1"
                     /translation="YSDLSEYGISDNSYKPPSQESESRKKRNLLFEKYGNFDPNNEFW
                     MKVVPTNYLGGLDGVVQINHAVDDPVVSIEYTRNLSSVLLETTLKHEINEYPTDGHNI
                     SGSSFDKAMQNTVDFFKRTLN"
     gene            2501..3676
                     /locus_tag="P5014_01650"
     CDS             2501..3676
                     /locus_tag="P5014_01650"
                     /inference="COORDINATES: protein motif:HMM:NF023802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HRY56847.1"
                     /translation="MKKFIFMLVFLLTAVIGITLFYKINQNPPLDTKFVQNVRDNEQN
                     TNNLTPKTEGKGSTMQSESNPSLEVEPNPVSLKVPGEYAQLTNNYHVYQSFNNCGPAT
                     LSMAMAWYGVKVPQEELASKMRPFQHPKGDNDDKTIFTYEFVNWANEYGLEAIGRVNG
                     DIDLLQKFISNGIPVVVKTWLKPNEDIGHFRFVTGYDEDKQIIVQDDSYEGPNKKIGY
                     YDFLTMWQAFNYSYVVVYTPELKEKVESIIGEEVDEKIAWENSLRRAEEESLKEDKNP
                     YPHFNISTASYHLGDYERSVKEYEKVENVLPKRMLWYQIEPIKAYQELKNYDKVFEII
                     EKILENGNRSFSELYMIRGEIYLEQGFKDKAKEQFELALKYNINMEEAESALKIWNN"
     gene            complement(4091..4876)
                     /locus_tag="P5014_01655"
     CDS             complement(4091..4876)
                     /locus_tag="P5014_01655"
                     /inference="COORDINATES: protein motif:HMM:NF018649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HRY56848.1"
                     /translation="MSYETFNLIVTVPLSIFVYMSLCFLFSKYLKRTDFVDIAWGFGF
                     ILVAVLTLFISRYSNVISIITTLLVLIWGIRLSTYLFLRFRKTKEDLRYVEIKKNWKK
                     NKLLKEFLGIFLLQGLLMYIVSLVVSVINIIGPGNNNPFYYLGLLVWITGFVFESVAD
                     AQLKNFIKTKKGKDKKIMDEGLWKYSRHPNYFGEVTMWWGIFLISVGTNYWPITIISP
                     VTITYLILKVSGVPLLEKKYQDDPDFRKYAKKTSIFFPLPPKK"
     gene            complement(4877..>5063)
                     /locus_tag="P5014_01660"
     CDS             complement(4877..>5063)
                     /locus_tag="P5014_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014553445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2177 family protein"
                     /protein_id="HRY56849.1"
                     /translation="KSSTLAIQLGAFLGFIAYATYDLTNLATLKNWPLHVTVIDIVWG
                     TFLTGSVSFLSYLILKK"
BASE COUNT         1831 a          732 c          868 g         1532 t
ORIGIN      
        1 gatacacaat catcaaaaaa tattttagat ttgttgaaag atttaaataa aaagttggga
       61 caaacaataa ttatggtaag tcatgaacct gaagataaaa aatatgcaga tcgaatttta
      121 tggctagaag acggtcttat agttaaagat gagaaagtta ggtaataaat ttatggagat
      181 aaaaaaatga aattaataag taaaaaaaca ttaatatttt tagtaattat tttaatagtt
      241 ggctttggat tatttttccg ttctagaaaa aaagacacga aggagtatta cacagttcaa
      301 cggcaaaata tggagctgat agtagaaaca agtggctacg tacaatcaaa tgataaagca
      361 gatttgtatt ttcaagtagc tgaaacaata accgacgtat tagttgttga gggagatgct
      421 gttgagaagg gtgatatttt agcaactgtg agtgggcaat ctttagctca aagctctcag
      481 gtagccagag ataacaggga tattgcaata ttcgagaagg atttatatgt tgaaaaatat
      541 gctaccgaca cagatgcagt tggtggggat aatgagtacg accgacagtt aagaatatac
      601 gatgaaaata ttagtaaagc agaggcactg tatgatcaat cattaataaa tagtagaaaa
      661 tcagttatcg ttgctccttt taacggtaca ataagtaagg tttacgtgaa caaaggtgat
      721 ctttcttctg taagtcgtcc tgcaatggaa atacaagatg tatcaaaacc ggtgctttac
      781 acgaatatat ctgaagtaga tatttcttac acggatacaa gcgataaagt agagataatt
      841 tttgatgcct ttcctgataa agttttcacg ggatacatat attccataaa ccctgctcct
      901 gagacaattc agggagtatc gtattacaaa gctgagatta aagcagataa tcttcctgag
      961 aaactacgtg tcggaatgag tgctgacata aatatactag ctgaaaaaaa agagggtgtt
     1021 ttgtacctac ctctctattt aatggataat gtgactgcgg atactggtct agtaaaactg
     1081 gatggaaaca atgggagtga agagactgag ataaaaatag ggttgaataa ttacaagggc
     1141 gttgaggtca tttcaggatt gaatgagggt gatgttgtag tttatgatag agaatgatag
     1201 ttcccaacaa ccttccagat agtccaccag atctatcaca aatattatct gtgctttgct
     1261 atactctcat tagtgaattt agagaaatta aatatcagta aaaattcaaa agttctattt
     1321 atctatcccc atccagatga tgaaacctat tttaatgctg gactgattca gaatttagtt
     1381 aaagaaaata tcaccactag gattttatgt ttaactaaag gagaggcttc ttctcttaaa
     1441 tatgggatta agcataataa tttaggagaa ataaggaaaa aagagttcga atcggtcatg
     1501 aaatttttaa aagtttctga acacaagatt ttagattttg aagatggaaa tttaggtaat
     1561 gaaaattccc tttctgagat actagaaaaa gaaattaatt tatttgaacc tgatgtggta
     1621 ataactttcg aaccaagcgg agtatatggg cacagggatc atatcgtggt atcacaggtt
     1681 gtgactgatc tcaaaaaact tagaaaattt aatttaatat attctactgt tccatttaat
     1741 taccacacct cccaaaaaaa ctcattaaat tttaaaattg atagtaaacc gctagaaccc
     1801 gatatgattt taaaactgag tccaaatgaa gttgttaata aactaatatg tttaataaaa
     1861 tataagagtc aggtaaacaa cgaaagattg attcaaataa tcttaaagcc ttatttgtac
     1921 aaagaatgct attgtatcta cccaaatcta taagtaacgt aaactttcgg tgtttaaaaa
     1981 tgataaaata cctaataatt tttaattgct tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnnnn ntattcggat ttatcagaat acggtatttc tgataacagc tacaaaccac
     2161 cttctcaaga atcagaatcc aggaaaaagc gaaacctgtt atttgaaaaa tacggtaatt
     2221 ttgatccaaa taatgaattt tggatgaagg tagttcctac aaactatcta ggaggtttag
     2281 atggggtagt tcaaattaat cacgcagtgg atgatcctgt tgttagcatt gaatatacaa
     2341 gaaacttaag ctctgttctt cttgaaacca ctttgaaaca cgaaataaac gaatatccaa
     2401 ccgacgggca caatatttcg ggatcttctt ttgataaagc tatgcaaaat actgtggatt
     2461 tttttaagag aacactcaat tagtatataa tccagacaac atgaagaaat ttatatttat
     2521 gttagtgttc ctattaacag cagttatagg cattactttg ttttataaaa ttaaccaaaa
     2581 ccccccactt gatactaaat ttgtgcaaaa tgtgagagat aacgagcaaa atacaaataa
     2641 cttaactcca aaaactgaag gaaagggcag tacaatgcaa tccgaatcaa accctagctt
     2701 ggaagttgag ccaaatcccg taagtttgaa agtgcctgga gagtacgcac aacttactaa
     2761 caattatcac gtttatcaat cattcaataa ctgtggacct gcaacacttt ctatggcaat
     2821 ggcatggtac ggagtaaaag ttcctcaaga agagcttgca agtaaaatga gaccttttca
     2881 acacccaaaa ggagacaacg atgataaaac aatttttact tatgagttcg taaattgggc
     2941 taatgagtac ggacttgaag ctattggtag ggtaaacgga gacattgatc tgctccaaaa
     3001 atttatttca aatggaatac cagttgttgt aaaaacatgg ttaaaaccaa acgaagatat
     3061 cggacatttc agattcgtta ctggatatga tgaagataaa cagataatag ttcaagacga
     3121 ttcctacgaa ggtccaaaca aaaagattgg atattatgac tttttaacca tgtggcaggc
     3181 ttttaattat agctacgtgg ttgtatatac tccagaatta aaagaaaagg ttgaatcaat
     3241 aattggagaa gaagtagatg agaaaatagc ttgggaaaac tctttaagaa gagcagagga
     3301 ggagagttta aaagaagaca aaaatcccta cccacatttt aatatatcta cagcttctta
     3361 ccacttaggt gattatgaaa ggtctgtaaa agaatatgaa aaagttgaaa atgtattacc
     3421 taaaagaatg ctttggtacc aaattgagcc tatcaaagcg taccaagagt taaaaaatta
     3481 cgataaagtt tttgaaatta ttgaaaaaat acttgaaaat ggaaatagat ccttttcgga
     3541 gctttatatg atcagaggag agatttatct tgaacaagga tttaaagata aagctaaaga
     3601 acagtttgaa cttgctttaa aatacaatat aaatatggag gaggctgaga gtgcgttaaa
     3661 aatctggaat aactaataag gactagcata gaggttgttt ctcatatacc agtccttata
     3721 ttttccttat cggtataaaa aatcttagtt aagacctata ttagaacaca acagttctaa
     3781 gtattcatct atctgcatgt tgattaactt acgaatccct accaatactt tttcatatag
     3841 tacttcctct tctatatatt ttaactctga tacctcttta atagatggat cgattgtaaa
     3901 ttctatatca taatagatac tagaatccgg atggatatgg agatttaatt taggtatctc
     3961 caatgtaaac caaacaacct tcttgttcct atatccatag tacactggcc atctaagatt
     4021 catcttctct ccttttatgt tattgttcgg attataaatt aatagtattt aaaagacaat
     4081 tagattgtag ttactttttc gggggtaacg ggaaaaaaat actggttttt tttgcatatt
     4141 ttctaaaatc agggtcatct tgatactttt tttctaataa aggaacccca gaaactttga
     4201 gaataagata ggttatagtt acagggctga taatagtaat tggccagtaa tttgttccaa
     4261 cagaaatcaa aaatattccc caccacattg taacttctcc aaaatagttg gggtgtcttg
     4321 aatatttcca taagccttcg tccattattt tcttatcttt gccttttttc gttttaataa
     4381 aatttttaag ttgagcatca gcaactgatt caaaaacaaa tcctgttatc cagactaata
     4441 aacctaggta ataaaaaggg ttgttatttc caggtccaat aatattaatt acggatacta
     4501 ctaaacttac gatgtacatt aaaagtccct gcaaaagaaa tattccgagg aattccttca
     4561 gtaatttgtt tttcttccag ttttttttaa tttcaacata tcgcaaatct tcttttgttt
     4621 ttctaaatct caaaaaaaga tacgtggata atctaattcc ccaaattaaa acaagtaggg
     4681 tagtgataat tgatatgaca ttagagtatc tgcttataaa taaagttaag acagcaacaa
     4741 gaataaaccc aaacccccaa gctatgtcta caaaatctgt cctttttagg tattttgaaa
     4801 aaagaaagca aagagacatg tagacaaata ttgataacgg aactgttact attaagttaa
     4861 atgtctcgta actcatttat ttttttaata tcaaatagga caaaaatgaa acacttcctg
     4921 ttaaaaaagt gccccaaacg atgtctatta ctgtgacgtg aagaggccag tttttaagag
     4981 tagcaagatt cgttagatca taggtagcat aagcaataaa tcctaaaaaa gctcccagtt
     5041 gtattgctaa agttgatgat ttt
//
LOCUS       DAOTFA010000032         5028 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate AMR_MDS_3115
            NODE_12049_length_5028_cov_6.540921, whole genome shotgun sequence.
ACCESSION   DAOTFA010000032 DAOTFA010000000
VERSION     DAOTFA010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868139
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (wastewater metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 5028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:36:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 536
            CDSs (total)                      :: 521
            Genes (coding)                    :: 520
            CDSs (with protein)               :: 520
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5028
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12049_length_5028_cov_6.540921"
                     /isolate="AMR_MDS_3115"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1013)
                     /locus_tag="P5014_01665"
     CDS             complement(<1..1013)
                     /locus_tag="P5014_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56850.1"
                     /translation="MKELGLIIDLNQPLVNEESVIKRSITDFYNPLARLLKSYKNISF
                     SLNIPLSTLELWEKHGAAGLILDFKDMYQNEKFELINSSPYGLHLTRLPEKIIESQLI
                     LNEYGIGYYLGSRQGFEGEPSIMLRDVTGFLPVFNDLVDEGVLKVLLDFDYSWFVIKN
                     SNLKENLFEIDTGNRVIKGVNAVDDLNNIISRLLDNESGYIDSQSLDKHAFEEVNAVF
                     ERIYSDDNNSAVVVLGSGNDNVDIDINYRNLFRVYESVLDNLSKMFGQVKNVGDLLKY
                     KYKLKKYSVGDYLKEIKNITNDERIDFKRAEELCEKILNINSNIVPISNIDINSSDLS
                     AIK"
     assembly_gap    1301..1400
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1401..2234)
                     /locus_tag="P5014_01670"
     CDS             complement(<1401..2234)
                     /locus_tag="P5014_01670"
                     /inference="COORDINATES: protein motif:HMM:NF024139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRY56851.1"
                     /translation="MIKIENKLYSSTEVADILGVSLRSVYRYLEEGKLQAEVKTATGR
                     HRFTRQNIVDFLYPSGMPEEEMLEQRMSVSQKEEMRMPVSEARVEVEDAPKVSAPEPI
                     KEPEVSIPLAPQPKMEVETQKVEAEKEDDQVDWLAKFREAAKKFEETTEQFSGLGDIA
                     PEPEEIVPVMPKKEIYFYRSRLGGLKDVAQNIDKTSKNSGLDYAFTLNAGLSLYKPIN
                     PFSLLHVYVRPKDRDFFERILALTPSDEGNAQLCILVSDENELYAKADEMHGLKVVDK
                     KR"
     gene            complement(2300..3217)
                     /locus_tag="P5014_01675"
     CDS             complement(2300..3217)
                     /locus_tag="P5014_01675"
                     /inference="COORDINATES: protein motif:HMM:NF015748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0043163 - cell envelope organization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HRY56852.1"
                     /translation="MISRKILGVRVDFGLNMEKALDIIENNLLNDGKNHIVCTTNPEF
                     VVDAQEDSDFRKIINDSSLSVPDGIGVVLAKNYLNAVDTLKRNSLFPIMAFYKGVRVG
                     ISSFIHKDEVKEERISGVDLTYEICKLSSQKGYTIFFLGGRAKNAVGNFIDEGDKDMS
                     NKAADIMRDKYPGVNIVGATSSFSRDSKDDEKTVDYIKKVMVEKGINKIDFLFVAYNH
                     LHQEKWIMRNKDKIPAKVSIGCGGTFDFIVGNCSLPPDIYAKYNLGWLYRLIKQPWRI
                     KRIIKAFPIFPMKVYVDSISIRGNNALKK"
     gene            3261..4457
                     /locus_tag="P5014_01680"
     CDS             3261..4457
                     /locus_tag="P5014_01680"
                     /inference="COORDINATES: protein motif:HMM:NF019911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEGA domain-containing protein"
                     /protein_id="HRY56853.1"
                     /translation="MNPNKNDKKFKFKKLGYMETLLTLLIIVGISTLLYLYTSGYRLS
                     RNDEKGTLDVEKTGMIGVKSIPDRASVYIDGKVRTATDDTVAGITPGPHELKIIKKGF
                     NEWSKTIEVYEQLVTDITAVLVSQSPRLEPLTNTGAKYPSVSPSLTKLAYFSTDIEKP
                     GIWVIPLTGVNLGLFRTNPTVVIEDTSYTKYSDGGEIMWSPDEKNLLVKSLKGTFYIV
                     NLDSKTAQTTLKSEEILDEWVKEQNEKRKLFIDNSLVKIPQEVVDKALSKDAYWAPDE
                     KKFLYTKQNGNKLEYWVYNFEIPLPIGEKTESLVMTTDIDAPQPKINWYSDSFHFVIV
                     EGDVENEKKGTVSLIRIDGTNKTEIYNNTLYSDAAFSSPGGEKVIILTSFKSGEQTDL
                     YTVSIR"
     gene            complement(4505..>5028)
                     /locus_tag="P5014_01685"
     CDS             complement(4505..>5028)
                     /locus_tag="P5014_01685"
                     /inference="COORDINATES: protein motif:HMM:NF016119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HRY56854.1"
                     /translation="ENGEEEYSVFSSLLTKNTTLKIKPVNEDFSIVIEKIGVNAPIVP
                     NVSITNEKVYKEALETGVAHAISSDYPTTAPSNVYLFAHASLNFWDLGKYATVFNLLR
                     KLDLKDKINIFYKGDRYVYEVVNKEVVKGWNTTSITRPVIEPVLTLQTCDPPGTTINR
                     FVLTAKLIEKIEK"
BASE COUNT         1660 a          929 c          720 g         1619 t
ORIGIN      
        1 aatttaatag ctgataaatc actactattt atgtcaatat tagatatggg tacaatattc
       61 gaatttatat ttaatatttt ctcacacaac tcctctgcac gtttgaaatc tattctctca
      121 tcattggtaa tattttttat ctccttcaga taatcgccta ccgagtactt ttttaatttg
      181 tatttgtatt ttagaagatc accaacattt ttaacttgac caaacatttt acttagatta
      241 tctaagacag attcatatac tctaaataaa tttctataat taatatctat gtcgacatta
      301 tcattcccag aacccagaac tactacagcg ctattattat catcggagta tatcctttca
      361 aaaacagcat ttacttcctc aaatgcgtgt ttatcaagac tctgtgaatc gatatagcca
      421 ctctcattat ccaacagtct tgaaattata ttatttaaat catcaaccgc gtttacacct
      481 tttataactc tattaccagt atcaatttca aataaattct ctttcaagtt ggaattttta
      541 atcacaaacc atgaataatc aaaatccaaa agtactttca aaacaccctc gtctactaaa
      601 tcattaaaaa caggcaaaaa accagtaaca tctcttaaca taatagaggg ttcgccttca
      661 aatccttgcc tggaacctaa gtaatatccg ataccgtact catttaatat cagttgagac
      721 tcaattattt tctcaggcag tctagttaag tgtagaccgt atggagaaga attaataagc
      781 tcaaatttct cattctgata catatccttg aagtctaaaa tcaaaccggc agccccatgt
      841 ttttcccata attcaagtgt tgaaagtggg atgtttaatg aaaatgaaat atttttataa
      901 gatttcaata accgagctaa aggattataa aaatccgtaa tagacctttt tataacgctc
      961 tcctcgttaa ccaagggttg attaagatcg atgattaaac ctaactcctt cacaggtgaa
     1021 attatccatt aaaaaaatct ttatgtaaag aatgaaaaag aataaatgtg atgataaaaa
     1081 taatcaaata aaatatttgg aaacatagaa aatatgataa tgaaaatttg aaaatgaaaa
     1141 aaaacagagc ctgtcctgaa ataaaaaaga ccctaaccga aggagacggt ttattatacc
     1201 cctgcccacg gcataacaaa ctttagtgtc tcccccggat agggtctttc ttgttaatgc
     1261 gaggattata acaaaatact ataacgctgt caatcctaac nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn tctcttttta tctacaacct ttaaaccgtg catctcatca
     1441 gcttttgcat agagttcatt ttcatctgaa actaatatgc agagttgtgc gtttccttca
     1501 tccgaaggtg ttaaagcaag tattctttca aagaaatctc tgtctttagg tctaacataa
     1561 acatgaagta gtgaaaatgg atttattggt ttatataatg acaaacctgc attaagggtg
     1621 aatgcgtaat ctagtcccga attctttgaa gtcttatcaa tgttttgagc tacatctttt
     1681 agtccaccaa gtctacttct ataaaaatat atttccttct ttggcattac aggaactatt
     1741 tcctcaggtt ccggagctat gtcaccaaga cctgaaaatt gctctgtggt ttcttcaaat
     1801 ttctttgcag cttctctgaa ttttgctaac caatctacct ggtcatcttc tttttctgct
     1861 tcaacttttt gtgtttcaac ttccatttta ggttgtggtg ctaggggtat ggagacttca
     1921 ggctctttaa taggctcggg tgcggagact ttaggtgcat cctcaacctc gactcttgcc
     1981 tctgaaacag gcattctcat ttcttctttt tgagatactg acattctctg ctctaacatt
     2041 tcctcttctg gcatccctga aggatacaag aaatctacta tattttgtct tgtaaatctg
     2101 tgtctacctg tagcagtttt gacctctgct tgtaattttc cttcttctaa atatctgtaa
     2161 actgatctaa gacttacgcc taagatatct gcaacttctg tggagctata aagtttattt
     2221 tctattttta tcatactgaa atgaaaacat cttaaatgtt atatgtcaat gaatgaccgt
     2281 tatactaata gacgtgatat tatttcttta gcgcgttatt acccctaata cttatagaat
     2341 ctacatatac tttcatagga aatattggaa atgcttttat tatcctcttt atacgccacg
     2401 gctgttttat aagtctatat aaccaaccta aattgtattt agcatatata tcaggaggaa
     2461 gactgcaatt accaacaata aaatcaaaag tacccccaca accaattgat acttttgcag
     2521 gtattttatc cttatttctc attatccatt tttcttgatg taagtgatta tatgcaacaa
     2581 atagaaaatc tattttattt ataccttttt ctaccattac ttttttaata taatctacag
     2641 ttttttcatc atctttggaa tccctgctaa aggaagaagt agcacccaca atattaactc
     2701 ctgggtattt atctctcata atatccgctg ctttatttga catatcctta tcaccctcat
     2761 caatgaaatt gccgacagca ttttttgccc ttcctcccaa gaaaaaaatt gtgtatcctt
     2821 tttgagatga taacttacaa atttcatatg ttagatctac accagatatt ctctcctcct
     2881 ttacttcatc tttgtgaata aaagaagaaa taccaacccg tactccttta taaaaagcca
     2941 tgataggaaa aagagaattt cttttcaaag tatctactgc gttcaaataa ttcttcgcta
     3001 aaacaacacc tataccgtcc gggactgaca gtgacgaatc gtttattatt tttctaaaat
     3061 cagaatcctc ttgtgcatct acaacaaatt caggattagt cgtacacact atgtgattct
     3121 tcccatcatt caacaaatta ttttcaataa tatccaaagc tttttccata ttaagtccga
     3181 agtcgactct tacacccagt atttttctag aaatcataaa acaattatac cttaataata
     3241 ttgtgataaa attatggaat atgaatccaa acaaaaatga taagaaattt aaatttaaaa
     3301 aacttggcta catggagaca ttactcactc ttttaatcat tgtgggtata tcaacactac
     3361 tctatctata tacttccggt tatagattaa gcagaaacga tgaaaaaggt accctggatg
     3421 tagaaaaaac aggaatgatt ggagtaaaat ctatacccga cagagcctcc gtctacatcg
     3481 acggtaaggt aagaactgct acggatgata cagttgccgg aattacacca ggaccccacg
     3541 aattaaaaat tataaaaaaa ggttttaacg aatggagtaa aacaattgag gtctatgaac
     3601 agctagttac agatataacc gcagttcttg tttctcaaag cccaagatta gaacctttga
     3661 caaatacagg tgcaaaatat ccttcggttt caccttcttt aactaaattg gcatattttt
     3721 caactgatat tgaaaaaccc ggtatttggg tcattcctct aacaggagta aacttaggac
     3781 tttttaggac gaaccccact gtagtaatag aagatacttc ctacaccaaa tactctgatg
     3841 gaggagaaat aatgtggtca cccgacgaaa aaaacctgtt ggtaaaatct ctcaagggca
     3901 ctttttatat agtgaatctt gactctaaaa ctgctcaaac tactttgaag tcggaagaaa
     3961 ttttagacga atgggtaaaa gaacaaaatg aaaaaagaaa actctttata gataactctt
     4021 tggtaaaaat tcctcaagaa gtagtagata aagcactttc aaaagatgct tactgggcac
     4081 ctgacgaaaa aaagtttttg tataccaaac aaaatggaaa taaacttgaa tattgggtat
     4141 ataactttga aataccactg cctattggag aaaaaacgga atcgttagtt atgactaccg
     4201 acatagatgc tcctcaaccc aaaataaact ggtactcaga cagctttcac tttgtaatag
     4261 tagaaggcga tgttgaaaat gaaaagaaag gaacggtaag tcttatcaga attgacggta
     4321 ctaacaaaac agaaatttat aacaacacac tttactcgga tgccgctttc agttctcctg
     4381 gaggtgagaa ggttataatt ctaacttctt ttaaatccgg tgaacaaact gatttatata
     4441 cagtaagcat aagataagaa aattcataag aactaagcgg gagagagttt ataaaaacac
     4501 acttttactt ctctattttc tcaattaact ttgcagttaa tacaaatctg tttattgtgg
     4561 tgccgggagg atcgcatgtt tgaagggtca aaacaggctc tataaccggt cttgtgatag
     4621 aagttgtatt ccagccctta acaacctctt tatttaccac ttcataaaca tatctgtcac
     4681 ctttatagaa aatattaatt ttgtctttaa gatctaattt tctaagtaaa ttaaaaacag
     4741 ttgcgtattt accaagatcc caaaaattta acgaggcgtg agcaaacaaa tacacattac
     4801 tcggtgctgt tgtggggtaa tcagaagata ttgcatgagc tactccggtc tccagagcct
     4861 ctttataaac cttctcgtta gtaatagaaa cgttgggtac aataggagca ttaacaccaa
     4921 ttttctctat aacaatagaa aaatcctcgt taacaggttt tatttttaaa gtggtatttt
     4981 tagttaaaag gcttgaaaaa acactgtact cctcttctcc gttttctg
//
LOCUS       DAOTFA010000033         4975 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate AMR_MDS_3115
            NODE_12224_length_4975_cov_6.343089, whole genome shotgun sequence.
ACCESSION   DAOTFA010000033 DAOTFA010000000
VERSION     DAOTFA010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868139
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (wastewater metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4975)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4975)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:36:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 536
            CDSs (total)                      :: 521
            Genes (coding)                    :: 520
            CDSs (with protein)               :: 520
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4975
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12224_length_4975_cov_6.343089"
                     /isolate="AMR_MDS_3115"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..286
                     /locus_tag="P5014_01690"
     CDS             <1..286
                     /locus_tag="P5014_01690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56855.1"
                     /translation="PISCVRNGVPAGITLYGYEQLGLNIIRIMYRDNNPPLFSGLGIG
                     LLEDDSLFFLLKSGIELYLILNGPKDIISLDYFSVFEIKKEDENNITIKY"
     gene            complement(353..1153)
                     /locus_tag="P5014_01695"
     CDS             complement(353..1153)
                     /locus_tag="P5014_01695"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF000595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HRY56856.1"
                     /translation="MTFQEITQYERKLYNQGVKLIAGVDEVGRGPLAGPFVVSSVILN
                     LEKIFSKDFQRDVEESWGRVVSYKYFMENTGKNEELNTEVTTRETSRNNDVLLRHTEK
                     KETTLYGKIRDSKKLSPKTREILSEFIKKEAISYSIEVFGPEEVDKLGISKLTQMAFF
                     QAIQRLKVKPQYVLTDTFEVVKITKQNQKNIVNGDNKSISIASASIVAKVFRDSIMIK
                     MHEKYPNYGFDKNKGYGTAIHLKALKEFGPCEIHRKSFEPIKSLEISR"
     gene            complement(1200..3776)
                     /gene="gyrA"
                     /locus_tag="P5014_01700"
     CDS             complement(1200..3776)
                     /gene="gyrA"
                     /locus_tag="P5014_01700"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008578936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HRY56857.1"
                     /translation="MAKETNKTEEIIEPQDNPNILRTEITTEMESSYLDYAMSVIVSR
                     ALPDVRDGLKPVQRRIIYSMQDSGINFSGKFHKCASVVGNVLAKYHPHGDTSAYEALV
                     RMGQDFSLRYPLIKPQGNFGSIDGDPPAAYRYTECKLEKISEELYSDIDKDTVNFEIN
                     DLQNLEPVYLPSNLPNLLLNGTTGIAVGMATNIPPHNLGEIVEGINILIDKSENIGEA
                     PKKDDKVQIVKPDFSSEANVEDLTKVILGPDFPTGGIIYDQKEMIQMYATGRGRVVTR
                     AKMDVEETKNGKVRLVVTEIPYAVNKSTLISKIADLVKEKRIIGISDLRDESNKDGIR
                     IMIELRKDAVVNKIKNQLYKYTQLQNTFNSNFVALLNNEPKLMTLKNILEEFVKHRQQ
                     IVIRRTIYLLKKAEERAHILRGLKIALDNLDEVIKLIRASKDADAAKEGLMKKFGLSD
                     LQAQAILDMQLRRLAALERQKIEDELKEILATIADYEDLLKSTKRIIKTVKDELNELG
                     KKYSDPRKTKVIKGKIGELSDEDLIVDEKCIVTISENGYIKRLKETTYKKQGRGGKGV
                     KGQELREEDAVETIKICNTHDWAFFFTNTGKVYKLRIWEIPESSRNAKGTPLINFLSM
                     KQEERVEAFLTKTSEELESKKGFVFFTTTHGVVKKTSMEDFANIRTSGIIAITLDKGD
                     NLVFVDSTSGQDDILLTTSMGQSIRFSEKEVRQMGRSAKGVTGIKLSKEKDSVVGTVI
                     IPNKSSDVELLVISKLGYGKKTKTGEYKTQKRAGSGILTYKVKEKTGELVVARAIDKG
                     TEYDALIATTSGKIIRLSLKQIPTLGRATQGVRLIKLGERDKVASVAIMGQEEQEMVQ
                     EE"
     gene            complement(3820..4749)
                     /locus_tag="P5014_01705"
     CDS             complement(3820..4749)
                     /locus_tag="P5014_01705"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HRY56858.1"
                     /translation="MNKIGVTQAIYTSDMDSLACNIAYTELLNLEGKNATAYVPKDPA
                     HTVTKEIKSWDFKYETEPSKENLQDLDFVLVDTTDPHTFPKFVKEEKIIEIYDHHVGY
                     EDYWIKKLGDRAKIETIGSCATLIWEEFKKRGFGEKISQTSAKMLYAAIISNTLNFKA
                     SITSHRDVEAFQEISIIADMPKGWVEKYFIDQEEYIYQNPRQAILDDIKIIEFPNMGT
                     KFTIGQIELWESKKFVNDYKPLIKEVLDNFDHKKWFFNAPCISMGKTYVLTENEEIRN
                     ILENNFGFIFKNEIGESDKLIERKEFVKVFLEL"
     gene            complement(4761..>4975)
                     /locus_tag="P5014_01710"
     CDS             complement(4761..>4975)
                     /locus_tag="P5014_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005702463.1"
                     /note="negatively supercoils closed circular
                     double-stranded DNA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HRY56859.1"
                     /translation="IVQRFKGLGEMNPEELWETTMNPETRRLKKITVKDAEAADEVFT
                     TLMGEEVAPRKRFITTHAHEANIDIT"
BASE COUNT         1256 a          974 c          777 g         1968 t
ORIGIN      
        1 cccaatatct tgtgtgagaa atggtgtacc ggcgggcatt accttatatg gctatgaaca
       61 attgggccta aacattatca gaataatgta tcgggataac aacccgcctt tattttcagg
      121 attagggata ggcttactag aagatgattc tctgtttttc ctgttaaaat caggtattga
      181 attatatctg attctgaatg gtcccaagga tattatttct ttagactatt tttctgtgtt
      241 cgaaattaag aaggaggacg agaataatat tactatcaaa tattaaatat tcctagatga
      301 ggaggataca aagttaaaag taccctcctc tctacactct ttaaaaaatc tttcatctac
      361 ttatttctaa tgattttata ggctcaaaac tttttctatg aatttcacac ggtccaaatt
      421 cttttagagc ttttaaatgg atggcagttc cgtatccttt atttttgtca aatccataat
      481 taggatactt ttcatgcatt tttatcataa tgctatctct aaaaaccttt gctactatac
      541 tcgctgaagc tatggatatg cttttattgt ctccatttac aatatttttc tgattctgtt
      601 ttgttatttt gacaacctca aacgtatctg tgagtacata ttgtggtttt actttgagcc
      661 tttggattgc ctggaaaaag gccatctgcg ttaatttaga aattcctaat ttgtctacct
      721 cctctggtcc gaaaacctct atagagtaac taatagcctc ctttttaatg aattcagata
      781 gaatctctct cgttttcggg ctaagtttct ttgaatctct tatttttcca tataaggtag
      841 tttctttttt ttcagtatgc cttaatagaa cgtcgttatt tcttgaggtc tctcttgtgg
      901 ttacttctgt gttcaattct tcattttttc ctgtattttc cataaaatat ttataagaca
      961 ctactctacc ccagctttct tctacatccc tttgaaaatc ctttgagaat attttttcta
     1021 aatttaaaat aactgaagaa acaacaaacg gtcccgcaag aggccctctg cctacttcat
     1081 caactcctgc aattagtttt acgccttggt tgtaaagttt tctttcatat tgagtaattt
     1141 cttgaaaagt cattggatat taaaattact tgtatttgac agtattgctt accttgagat
     1201 tattcttcct gaaccatttc ctgctcttct tgtcccatta ttgcaaccga cgcgacctta
     1261 tccctttctc ctagttttat tagtcttacc ccttgagttg ctcttcctag agtaggaatt
     1321 tgtttcaacg aaagtctaat aatctttccg gatgtagtag ctatcaatgc atcgtattcg
     1381 gtgcccttat ctattgccct tgcgacaacc aattctcctg ttttttcttt gactttgtaa
     1441 gttaggatcc ccgaccccgc tcttttttgg gttttatact ctcctgtttt tgtctttttt
     1501 ccgtatccaa gtttggaaat taccagtaat tcaacgtcac tactcttatt gggtataatt
     1561 actgtaccta ccacactgtc tttctctttt gatagtttta ttcctgtaac accttttgca
     1621 gatcttccca tttgtctaac ctctttttca ctaaatctta ttgattggcc catactggta
     1681 gttaaaagta tatcgtcctg acctgaagtg gagtccacaa aaactaagtt gtctcctttg
     1741 tctaaggtta tagcgataat tccagaagtt cttatattag caaaatcctc catagatgtt
     1801 tttttaacaa ccccgtgcgt tgtagtaaag aaaacgaaac cttttttgct ttccaactcc
     1861 tcgcttgttt tagttaggaa ggcttccact cgttcttctt gtttcatact taaaaagtta
     1921 atcaagggag tacccttggc atttcttgat gattcaggta tttcccagat tcttaattta
     1981 tatactttac ctgtatttgt gaagaaaaat gcccaatcgt gggtgttaca aatttttata
     2041 gtttcaacag cgtcctcttc ccttagctcc tgtcctttta ctccttttcc gcccctaccc
     2101 tgcttcttgt aagtagtttc tttaagtctt tttatgtatc cattttctga aatagtaact
     2161 atacattttt catctactat caggtcttcg tcgcttagtt ctcctatttt tccttttatt
     2221 acctttgttt ttctcggatc tgagtacttt ttacctaatt catttaattc atcctttact
     2281 gtttttataa ttctcttggt tgattttagt aaatcttcat aatcggctat tgtggcaagg
     2341 atttctttta actcgtcctc tattttttgt ctttctaacg ccgcaagtct tcttaactgc
     2401 atgtccaaaa tggcttgtgc ttgtagatcg gataaaccga actttttcat taaaccttct
     2461 ttggcggcat ccgcatcttt gcttgctcta ataagcttta taacttcatc taaattatca
     2521 agagcaattt ttaaacctct taatatgtgg gctctttctt cagctttttt gagtaaatat
     2581 attgttcttc ttattacaat ttgctgtctg tgtttaacaa attcttctag aatatttttt
     2641 aaagtcatta gttttggttc attatttaaa agagcgacga agtttgagtt aaaggtgttc
     2701 tgtagttgag tatatttata gagctggttc ttgattttgt tgacaacagc gtcttttctt
     2761 aactcaatca ttatcctaat cccatcttta tttgattcat ctcttaggtc agatatgccg
     2821 attattcttt tttcttttac caagtcagca atttttgaaa ttagagtcga tttattaact
     2881 gcataaggta tttcagtgac tacaagtctt acctttccgt tttttgtctc ttctacatcc
     2941 atttttgccc ttgttacgac tcttcctctt ccggtagcat acatttgaat catttctttt
     3001 tggtcgtaaa taattcctcc ggttgggaaa tcagggccta aaataacttt agttaaatcc
     3061 tcaacgttag cttcagatga aaaatcaggt tttactattt gaaccttgtc gtctttttta
     3121 ggcgcctctc caatattttc ggatttatcg attaatatat ttattccttc tactatttcc
     3181 ccaagattat gtggggggat atttgttgcc attccaacag caatacctgt agtaccgttt
     3241 aatagcaaat taggaagatt tgaagggaga taaaccggct ccaggttttg aagatcgttt
     3301 atctcaaaat ttacagtatc tttatcaata tctgaatata actcctctga tattttttct
     3361 aatttacact ctgtatatct gtatgcagct ggtgggtcac cgtctatact accgaagtta
     3421 ccttgaggtt ttattagagg gtatctcaag ctaaagtctt gtcccattct aacgagcgct
     3481 tcgtaagctg aagtgtctcc gtgtgggtgg tattttgcca aaacattacc tacaacacta
     3541 gcgcatttat gaaacttacc tgaaaagtta attcctgaat cctgcatgga atagataatt
     3601 cttctttgta caggttttaa gccgtctctg acatcaggaa gtgctcttga aactataacg
     3661 ctcatagcgt agtcgagata agagctctcc atctcggttg ttatctctgt ccttaggata
     3721 tttggattat cctgtggttc gattatctct tctgttttgt ttgtttcttt ggccataaac
     3781 ctaataatat tagcagattt gaactaaatt tgaagtcttt tataactcta aaaagacctt
     3841 tacgaactct tttctttcaa ttaatttatc cgactctccg atttcgtttt taaatataaa
     3901 accaaaatta ttttctagaa tatttcttat ctcctcgttt tcagtgagaa cgtatgtttt
     3961 tcccattgat atacaagggg cgttaaaaaa ccattttttg tgatcaaaat tatccaaaac
     4021 ttcttttata agtggtttgt agtcatttac aaatttttta ctttcccaaa gttctatctg
     4081 accgatggta aattttgtac ccatatttgg gaattctatt attttaatgt catccaggat
     4141 tgcttgtctt ggattttgat aaatatattc ttcctgatct ataaaatatt tctctaccca
     4201 gcctttgggc atatctgcaa ttattgatat ttcttgaaag gcttccacat ctctgtgaga
     4261 agtgatggat gccttgaagt ttaaagtatt ggaaattatg gcggcgtaaa gcatttttgc
     4321 gctggtctgg gaaatttttt caccaaaacc tcttttttta aattcttccc aaattaaagt
     4381 tgcacaggac cctatcgttt caattttagc tctatctccc agttttttaa tccagtagtc
     4441 ttcgtaaccc acatggtggt catatatttc aataattttt tcttctttta cgaatttcgg
     4501 aaaagtgtgc ggatctgtag tgtcaaccaa tacaaagtcc aagtcctgaa gattttcttt
     4561 tgaaggttct gtttcgtatt taaagtccca cgattttatt tctttagtga cggtgtgggc
     4621 ggggtctttg ggtacatatg ctgttgcatt tttcccctct aagttcaaaa gctccgtata
     4681 ggctatattg caggccaaag agtccatgtc tgaggtgtat atagcttgtg taactccgat
     4741 tttgttcatt ttaatcctct ttaggtaata tcgatattgg cttcgtgtgc atgtgtggtg
     4801 ataaatcttt ttcttggagc aacttcttcc cccatcaatg ttgtaaatac ctcatcggcg
     4861 gcctccgcat cttttacggt tatcttttta agtctcctag tctcgggatt catcgtagtt
     4921 tcccacaatt cttcggggtt catttctccc aaccctttga atctttgaac aatgg
//
LOCUS       DAOTFA010000034         4968 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate AMR_MDS_3115
            NODE_12254_length_4968_cov_6.257073, whole genome shotgun sequence.
ACCESSION   DAOTFA010000034 DAOTFA010000000
VERSION     DAOTFA010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868139
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (wastewater metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4968)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4968)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:36:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 536
            CDSs (total)                      :: 521
            Genes (coding)                    :: 520
            CDSs (with protein)               :: 520
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4968
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12254_length_4968_cov_6.257073"
                     /isolate="AMR_MDS_3115"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /locus_tag="P5014_01715"
     CDS             complement(<1..593)
                     /locus_tag="P5014_01715"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HRY56860.1"
                     /translation="MSKSKTKAKPKTPKNKTPKSQKKLNEKKLKTELNKNQLEAVNHE
                     SGPMLVVAGAGTGKTRVITERIKHLIQDKNVDPQEILALTFTEKASHEMVERVGDIMP
                     LGYEEPWIYTFHAFAERVLKEKGIEIGLDPSYKTLPDSDQWLLLRKNIFEMDLKYFRP
                     LGNPTKFISDILTFISKLQDENISNKDFETFVKQMKH"
     gene            687..3302
                     /locus_tag="P5014_01720"
     CDS             687..3302
                     /locus_tag="P5014_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56861.1"
                     /translation="MSVINKNLLKKFECSFFKLSLVFLFFIFIFGYSPISKKAYAYDI
                     YYIEDLYNIRYDLAGNHVLMNDLDFNSDTSYSDPNNKISYTAGEGWDPIQGFQGTFDG
                     SGYTISNLFISDSSSSIGVGLFGDISVSSSVLDVGLSNTYISAINSNKVGGLIGNNDG
                     RVVNCYSTGSVVGALWVGGLVGENDVNGVVTYSYSSAVVDGSDDTGGLVGYNRGDITN
                     SYSTGIVTGNGETGGLVGFSDNVGHISKTYSTGQVLYFPSGTGSGGSGGLVGLYEGML
                     ALSDSFWDTETSGMATSDGGIGKTTLEMQSVVTFTNWDIVDISSFDPYNPNDWYIDEG
                     VDYPRLFWESADYPPLPPEVDYTIDSIEDLDNIRYDLAGDYVLTRDLDFNSDASYSDP
                     ANKISYTTGEGWDPVGIFGAGSWFSGNFDGDGYIISNLYINNTTDEDVGLFGVAYYSN
                     ISNIGIVNANITSTSYAGILVSYYIGTGIIEDSYSTGSVTGSYEIGGLVSYNLGSINN
                     SYSTANVTGHSGLGGLVGYNEGSINNSYSRGSISGHDSYGGLVGENNGSITNSYSTGV
                     IESPENTGGLVGINGDAVGTVTDSFWDTETSGIDVSDGGTGKTTVEMMSLLTFDISAP
                     DENPSPWDIVKIENFDEEDPNIWYLDEGNDYPRLFWEFEGYNPPDDEHNEPSGRPVFH
                     PDKRCHWDKPKSPNWIKLEPMVKDGVSGMNLTWVQYDADKINIKIDDGTGKYPWKIEK
                     TSNDGHEFLPNVSSWQNIMIEPINHCKEGEYSLAVSQLTYPYGWYGQSDNKVALATNT
                     LGYSTKSSVLGTSTESEETPSEENPMVPETGSNDILYLTTSLSIIGFSIYFVLNEKSR
                     RFALRDFEKRVSRGL"
     gene            complement(3652..4710)
                     /locus_tag="P5014_01725"
     CDS             complement(3652..4710)
                     /locus_tag="P5014_01725"
                     /inference="COORDINATES: protein motif:HMM:NF014680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HRY56862.1"
                     /translation="MDIQTITLLAIIMIGSVILLGFYLSKQFKSSGKSEDTILLEWLK
                     DMKGTVEKNTEVMERQLKDQRSSLEEQMKNQRETLSQQTKFISERLEKSSDVIREVQK
                     QLGGIQEFGTDMKDLSNILKSPKLRGGLGEQFLYEILENFLPKDLFKTQYKFKDGSVC
                     DAVVFTDKGIIPIDSKFPMENFKAMITAEMQTERDKFKKTFVSDVKKRIDEISGKYIL
                     PEEKTTDQAIMYVPSENVYYELIVNTPEIEDYSKAKNVILASPNTLSYFLKVLLVAYQ
                     QHELQKHTGEILKALAGIKVEAGKFNEDLNVLERHISNSYKSMDGVKSRFARLYGRID
                     KAQAIEKTGKEEQPELLE"
BASE COUNT         1441 a          778 c          986 g         1763 t
ORIGIN      
        1 tcgtgtttca tttgcttaac aaaggtttca aagtccttat tagaaatatt ttcgtcttga
       61 agtttagaga taaaagttaa aatatctgaa ataaatttag ttggatttcc caaaggtctg
      121 aaatatttca aatccatttc aaaaatattt ttacgcaata aaagccactg atctgaatcg
      181 ggaagtgttt tataagaggg gtccaaccca atttcaattc ctttttcttt caaaactctt
      241 tctgcaaaag catgaaaagt ataaatccaa ggctcttcgt atcctaaagg cataatatct
      301 ccgacacgtt ccaccatttc atgcgaagcc ttttcagtaa acgtcagggc cagtatttcc
      361 tgaggatcaa catttttatc ttgaattagg tgttttattc tttcagtgat tactctggtt
      421 tttcctgtac ctgcgcccgc tacaactaac attggccccg attcgtgatt tactgcttca
      481 agttgatttt tgtttaattc ggtctttagc tttttttcat ttagtttctt ttgagattta
      541 ggtgttttgt tttttggtgt tttaggtttt gcttttgttt ttgatttaga catcttgggt
      601 atattttaca ttaaaaaata tataaaaagg gtggtttgtc ataatactat tgactgtgaa
      661 aaatataccc aatacaattt atatcaatgt ccgtcataaa taaaaacttg ctaaaaaaat
      721 ttgagtgtag tttttttaaa ttatctttag tttttctctt cttcattttt atatttggat
      781 attctcctat ttccaaaaaa gcttatgcat atgacatata ttacatagag gatctgtata
      841 acataagata cgatttggca ggtaaccatg ttttgatgaa tgatttggat tttaacagcg
      901 ataccagtta tagcgatcct aataataaga tttcgtatac tgctggggag ggctgggatc
      961 ctattcaagg ctttcaggga acatttgatg gaagtggtta tacaatttca aatctattca
     1021 taagtgactc atcttcttct attggcgtcg gtttatttgg agatatttct gtaagttctt
     1081 cagttcttga tgttggttta tctaatactt atatctcagc gattaattcc aataaggttg
     1141 gtggtctgat tggtaataat gatggtaggg tagtcaattg ctattcaact ggaagtgttg
     1201 tcggagctct atgggtaggt ggtttggttg gagagaacga tgttaacggt gtagtaacat
     1261 attcttattc ttctgctgtt gtagatgggt cggacgatac aggaggtctg gttggttaca
     1321 atcgtgggga tattacaaac tcctattcaa caggaattgt caccggaaac ggcgagacgg
     1381 gtggtttggt aggtttttct gacaatgttg ggcacataag taaaacctac tcaacaggtc
     1441 aagtattata ttttccgtct ggaactggaa gtggggggag tggtggttta gttggattat
     1501 acgaaggtat gttagcatta tcggattcat tttgggatac ggaaacatcc ggtatggcca
     1561 catctgacgg aggtatcgga aaaacaactt tggagatgca gtccgttgta acttttacca
     1621 actgggatat tgtagatata tcaagtttcg acccttataa tcctaatgat tggtatatag
     1681 acgagggtgt agattaccca agattgtttt gggaatctgc tgattaccca cccctaccac
     1741 ccgaagtaga ttacaccata gactcaatag aagatttaga taatattaga tacgatcttg
     1801 caggtgatta cgttttaact agagacttgg attttaacag tgatgctagt tacagtgacc
     1861 cagctaataa gatttcatac actactggag agggttggga ccctgttgga atctttggcg
     1921 ctggttcttg gttttctgga aattttgatg gagatggcta tataatatct aacttgtaca
     1981 taaataatac taccgacgag gatgttggtt tatttggtgt agcatattat tccaatatat
     2041 ctaacatagg aatcgttaac gcaaacatta cctcaacctc atatgcaggt atcttggttt
     2101 cgtattacat cggtactgga ataattgagg attcatattc aacaggctca gtaaccggct
     2161 cttatgaaat tggggggctg gtttcatata accttggatc tataaataat tcttactcca
     2221 cagctaatgt tactgggcac agtggtctgg gtggcttagt aggatataac gaggggtcaa
     2281 taaataactc ttactcaagg ggttccataa gtggtcacga ctcatatgga ggtttggttg
     2341 gggaaaacaa tgggtcaata actaactcgt attcaacagg ggtgatagaa agcccagaaa
     2401 atactggagg tttggttgga attaacggag atgcagtagg tactgtaaca gactctttct
     2461 gggatacaga gacttctggc atagatgtgt ctgatggtgg tacaggaaaa accacagtcg
     2521 agatgatgtc gttattaacc tttgacatat cggctcctga tgaaaaccca tcaccttggg
     2581 acattgtaaa aattgaaaat tttgatgaag aagaccctaa catctggtat ttggatgaag
     2641 gaaacgatta tccaagatta ttttgggaat ttgaaggcta caatccccct gatgatgagc
     2701 ataacgaacc atcaggtagg cctgtatttc atcctgacaa aagatgtcat tgggataaac
     2761 cgaaaagtcc taattggatt aaattagaac ctatggttaa agatggagta tcaggaatga
     2821 atttaacgtg ggtacagtac gatgcagata aaattaacat taaaatcgat gacggaacag
     2881 gtaagtatcc ttggaaaatt gaaaaaacat caaacgatgg gcatgagttt ttaccaaatg
     2941 tatcttcttg gcaaaacatt atgatagaac caattaacca ctgcaaggaa ggagaatatt
     3001 ctcttgctgt aagtcaactt acataccctt atggttggta tggtcaaagt gataacaaag
     3061 ttgctttagc tactaatacc cttggttatt caacaaaatc ttctgtattg ggaacctcta
     3121 ctgaatcgga agaaactcca tcagaagaaa acccaatggt acctgaaaca ggtagtaatg
     3181 atattttgta cctgactaca tcactctcaa taattggttt ttcaatctac tttgttttaa
     3241 atgaaaaatc cagaagattt gcattaagag attttgagaa aagagtatca agaggtcttt
     3301 aaaattaata actcactgag caaaaattca gtttttgtac tataatcttc caacaataaa
     3361 aaaggagaga gaatgttact gacaattgtt gctgaaagca attctgttca aattattaaa
     3421 ctagtcggct taattattgg tttcttacta attatagtat tggctaatct ttcaccacgt
     3481 aaggatatcg aggatggtga gaaaagtgag aacagcgttg catctgacga tactgattcc
     3541 aagtagtacc aaaagggact tttaagaaat tgaaggtccc tttctttttt aatctatatt
     3601 taaagaaggg aaacatatca aaaattcata gaatctatag aatctagaaa actactctaa
     3661 aagctcgggt tgttcttctt ttcctgtttt ttcaatagct tgggccttat caattcttcc
     3721 gtaaagcctg gcaaacctgc ttttaacacc gtccattgat ttgtaagagt ttgaaatgtg
     3781 tctttctaaa acgtttaaat cttcattaaa ttttcctgct tcaactttta ttcctgcaag
     3841 agcttttaaa atctctccgg tgtgtttttg aagttcgtgt tgttggtagg ctaccagcaa
     3901 aacttttaaa aagtatgaaa gagtgttggg agaggccagt atgacatttt tggcttttga
     3961 gtaatcttca atttcaggag tatttacaat aagttcgtaa taaacatttt cggaagggac
     4021 atacattatt gcctggtcgg tagttttttc ctcaggaagg atgtactttc ccgatatctc
     4081 gtctattctt tttttaacgt ctgatacgaa ggttttttta aatttatctc tttctgtttg
     4141 catttctgcc gttatcatag ctttaaaatt ttccatcggg aattttgagt ctattgggat
     4201 gattccttta tctgtaaaga ctacggcatc acaaacactt ccgtctttaa atttatattg
     4261 tgttttgaaa agatccttgg gaagaaagtt ttcaagtatt tcatataaaa attgttcacc
     4321 gaggccacct cttagcttag gggattttaa aatgttgctt aagtctttca tgtcagttcc
     4381 aaattcctga attccaccaa gttgtttttg aacttccctt atgacgtcgc ttgatttttc
     4441 aagtctttcg gaaatgaatt ttgtttgttg gctaagtgtt tctctttgat ttttcatttg
     4501 ttcttctagg gatgaacgtt gatcttttag ctgtctttcc attacctcgg tgtttttttc
     4561 aactgtacct ttcatgtctt ttaaccactc cagaagtatt gtatcttctg attttccaga
     4621 acttttgaat tgttttgaga ggtaaaatcc cagtagaata acggatccga tcattattat
     4681 ggcgagcagg gttattgttt gtatatccat agtttctatt ttaccagagt gttaaaatca
     4741 atctatgatt aagttggtga agttttctat ttttcttaat tttgttttgt gggttttgtg
     4801 gtttgttctt ttgttttttt ggatttcacc ggatacgtat atggagattt ttctgttctt
     4861 gggggttttg tttttatgtt tgggtttaag tttttcattt cctttttatt ttttttataa
     4921 aaaaaagtat ccgaagttta cagatttaaa gcagttgttt agaagggc
//
LOCUS       DAOTFA010000035         4908 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate AMR_MDS_3115
            NODE_12452_length_4908_cov_7.003709, whole genome shotgun sequence.
ACCESSION   DAOTFA010000035 DAOTFA010000000
VERSION     DAOTFA010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868139
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (wastewater metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4908)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4908)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:36:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 536
            CDSs (total)                      :: 521
            Genes (coding)                    :: 520
            CDSs (with protein)               :: 520
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4908
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12452_length_4908_cov_7.003709"
                     /isolate="AMR_MDS_3115"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..238)
                     /locus_tag="P5014_01730"
     CDS             complement(<1..238)
                     /locus_tag="P5014_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012530989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56863.1"
                     /translation="MKRTIKVNDLMQKNYIYELLEPIGKNFSPLFKPELTPKQMLELG
                     VFGGKYMTDCKKEFPKEWFEKAKLCHKFHDPKLNF"
     gene            341..1189
                     /locus_tag="P5014_01735"
     CDS             341..1189
                     /locus_tag="P5014_01735"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HRY56864.1"
                     /translation="MIKIVTDSGIDIPVDAPKEIADSITIVPLSFTIVGISHMDAGED
                     RLTTGEFLDLIEKSWPTTSAPSPESFAMVFREIVSKGDQVLCITISGGLSATKEAAVI
                     AARDFPYDVRVIDSKSVSLGMYYLVEYAKELISKPLSLHRIAKKLESKTRKIKSFVLV
                     KDLNNLLRGGRIGPTGYLIASLLSIKPLLQMNPDGKLREVSRGRTWKKAKEDMINKTL
                     SLTDMPNVVKGGVIHVQNLEEATQVANSLRNATGIDFDIAETGPVIATHTGRGALGVV
                     VVEIKR"
     gene            1451..1912
                     /locus_tag="P5014_01740"
     CDS             1451..1912
                     /locus_tag="P5014_01740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56865.1"
                     /translation="MFLRDSTFGSPDFLNLLKGDIDKVNQGLITNFNKIKENPNYDFI
                     DKEIILAEDDFHIEKVKFENELEMVVIKCPVPQKSPEATYIGIVTSDTPRYFTCEYES
                     KEETKQLYPEKEWAPEYILCEWVQKEHKNYGKIAETKEAFIEGVRKLISNP"
     gene            2039..2845
                     /locus_tag="P5014_01745"
     CDS             2039..2845
                     /locus_tag="P5014_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56866.1"
                     /translation="MPAELSTDDIKSVLNSMYEYGVHLGVDLKEILPDVYKILILPEQ
                     EYGSLVKRSEKYIPPDSNGYIRLGLEDIIVSDKLSRSKFQKLKTLTHELVHFASFRSY
                     ELGNRRATTTFQAGFSGRNQDEFRILDEGFTELTCWYVIKRFWKNQPNLTKYVSDLDR
                     HKLLVNYYRTVVLVDMIILKVARSLDMEYVDVLNQLQRLHIKNPPKGIKMIEEVIGSE
                     ALSVIAKLPSEIDSDDSDDVFLKTVKSLPIGKEFSEINDGSGVYKFWDRF"
     gene            3015..3578
                     /locus_tag="P5014_01750"
     CDS             3015..3578
                     /locus_tag="P5014_01750"
                     /inference="COORDINATES: protein motif:HMM:NF025224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HRY56867.1"
                     /translation="MNNKKTSLPLLLVVFVLGIASFWLYSSVIKSPKNPIKQVITNNN
                     EKEEKGEKEETQTPEISARKSLDLSGQGLEKLPSYVLSRTDLEELNISNNKLTGALPS
                     EIGKLTKLKVLNASNNLMTGVPAEVGHLPNLEVLDLSNNQLTGLPNELGQLTKLKVLN
                     LSGNDYSVQDLDIIIKGLPSDVNIIVD"
     gene            3675..3887
                     /locus_tag="P5014_01755"
     CDS             3675..3887
                     /locus_tag="P5014_01755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56868.1"
                     /translation="MAPMTKERFFQIAREYGLSDEVIQSQWDTRPADNIDEEKLRKAI
                     SKYNEVEQERRVHQATVSILKSHGIL"
     gene            complement(4365..4697)
                     /locus_tag="P5014_01760"
     CDS             complement(4365..4697)
                     /locus_tag="P5014_01760"
                     /inference="COORDINATES: protein motif:HMM:NF039191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5674 family protein"
                     /protein_id="HRY56869.1"
                     /translation="MVIVLDKKITHEQFKQACEAYSDYIKTVIDIEKNVLAIGGEYHI
                     DCEEVLIKSGSRLENLFGGGYRVSTKEVEYMAMSNYKPALGKNTYEITDPKVRQRLEN
                     LTKEFLEI"
BASE COUNT         1628 a          740 c          930 g         1610 t
ORIGIN      
        1 aaaagttcag tttagggtcg tgaaatttat gacatagttt tgctttttca aaccattctt
       61 taggaaattc ttttttgcaa tcagtcatat atttgccgcc aaaaactcca agttctaaca
      121 tttgcttagg tgtgagctcc ggcttaaaaa gtggggagaa gttttttcca atcggctcaa
      181 gaagctcgta gatgtagttt ttttgcatca aatcatttac ttttattgtt cttttcatat
      241 ttataaatta tatcaagtaa aaaattgttt atcggtgagt tttgaatatt tttaaacatg
      301 ctttataatc ctctaaaata gactagaaaa ggagaagaaa atgataaaaa tagtgacgga
      361 tagtggtatt gatattccgg tggatgcacc aaaagaaatt gccgattcga taaccatagt
      421 tcctcttagc tttacaatag taggaatttc tcatatggat gctggtgagg accgactaac
      481 cacaggggaa tttttggacc tgattgaaaa aagttggcct acaacgtcag cgcctagtcc
      541 agaatcgttc gcaatggttt ttagggaaat agtgtccaag ggagaccaag ttctgtgtat
      601 aaccatatca ggaggattaa gtgcaacaaa agaagcggct gtaatagcag caagagattt
      661 tccttacgac gttagagtaa ttgattccaa aagtgtttct ttaggtatgt attaccttgt
      721 tgagtacgca aaggagttaa ttagtaagcc tctaagcctt cacagaatag caaaaaagtt
      781 agaaagtaaa acaaggaaaa tcaaatcttt tgttttagta aaagacctga ataatctttt
      841 aagaggtggt cgtataggtc ctactggata ccttatcgcg tctttgctta gtattaagcc
      901 tctgttgcaa atgaatcctg acgggaaact tagagaagtt agcagaggaa gaacctggaa
      961 aaaggccaag gaggatatga taaataaaac actctcctta acggatatgc caaatgttgt
     1021 taagggtggg gttattcacg ttcaaaatct tgaggaggca acacaagttg ctaattcttt
     1081 gcgtaatgcc actggtatcg attttgatat cgctgaaacg ggccccgtta tagcaacaca
     1141 taccggaaga ggggctttgg gggtagtagt ggtagaaata aaaagatagt actttacaaa
     1201 accgacatcg gaaaaatgtc ggtttttctt ttacctctaa aacttcaaat tgttggataa
     1261 gttttacaat atgtactttt tttcaaaagg aacaatgtgt ttcggttgaa tataagatac
     1321 aagttcttcc agttaattta ttgaagtaaa aattgtgtgt ataataaata cgtgctgtat
     1381 tttatatcaa gtttttgaaa ggataaaaat gtccaacaat gctccaagcc ttttatatta
     1441 tttcgaacat atgtttctaa gggattccac atttggaagc cctgattttc ttaacttact
     1501 aaaaggagat attgataagg ttaatcaagg tctaattaca aattttaaca aaattaaaga
     1561 gaatcctaat tacgatttta tagataaaga aattattctt gctgaagatg attttcatat
     1621 agaaaaagtt aaatttgaaa acgagttgga gatggttgtt atcaaatgtc cagttcccca
     1681 gaaatcccca gaagccacat atattggaat tgtaacttct gatacacctc gttatttcac
     1741 atgtgagtac gaatccaaag aagagacgaa gcagctttat ccagaaaaag agtgggctcc
     1801 agaatatatt ttgtgtgagt gggttcaaaa agaacataaa aattatggaa aaattgctga
     1861 aacaaaagaa gcttttatag aaggtgtaag gaagcttata tccaaccctt gagatcttat
     1921 aacagtcttt aaagtattta aatttttagg cttgttggct aagacgatga ggtatgtgac
     1981 aatgtatttt gcaaaaaagt agttgtttct gatattttta aacctgtata atttagttat
     2041 gcccgctgag ttatctactg atgatataaa aagtgttctt aactctatgt acgagtacgg
     2101 tgtacacctc ggagttgatt taaaagaaat tttgccagat gtctataaga ttttaatctt
     2161 acccgaacag gaatatggta gtcttgttaa aagaagtgag aaatatatac ccccggacag
     2221 taatgggtat ataagactag gtttggagga tattattgtt agtgacaaat tatctcgaag
     2281 caagtttcag aaattaaaga cccttactca cgaattggtt catttcgctt cttttcgtag
     2341 ttatgaatta ggaaacagaa gggcaacaac tacctttcaa gcaggttttt ctggtagaaa
     2401 tcaagatgaa tttagaatcc ttgatgaggg ttttactgaa cttacgtgtt ggtatgtaat
     2461 aaaaagattt tggaaaaacc aacctaattt aactaagtac gtatcagatt tggacagaca
     2521 caaattatta gttaattatt atagaaccgt tgttttggta gatatgatta ttttaaaagt
     2581 tgctaggagt ttagatatgg aatatgtaga tgtattaaat caattacaaa ggctacatat
     2641 aaaaaatcct cctaaaggta taaaaatgat tgaggaagta attggtagcg aggccttgtc
     2701 tgtaatagca aaattgccaa gtgaaattga tagtgatgat agtgatgatg tgtttctgaa
     2761 aactgttaaa tctttgccta ttggaaaaga gttctcagag ataaatgatg gaagtggggt
     2821 ttataaattt tgggataggt tttaaccact catatattgt tataacttca acccacacac
     2881 acaggaaggg gggggctcta ggtattgtgg tagaaataaa aaaatagtac tttacaaaaa
     2941 ccgacatcga aaaaatgccg gtttttcttt tacctgcttt gcttaatggg ttaataaatg
     3001 ttaagtttaa ttttatgaat aataaaaaaa cttctttacc cttactttta gttgtatttg
     3061 tcttaggaat agcttctttt tggctttatt cttctgtaat aaaatctcca aaaaacccaa
     3121 taaaacaagt aattactaat aataatgaaa aagaagaaaa aggggaaaaa gaagaaactc
     3181 aaacaccaga aatatctgct agaaaatctt tggatttaag tggtcagggg ttagaaaaat
     3241 tgccatctta cgttttatct agaactgatt tagaagagtt aaatatttca aataataaat
     3301 taacaggtgc gcttccttct gagattggaa aattaactaa attaaaagtg ttgaatgcat
     3361 cgaacaattt aatgacaggg gttcccgctg aggtaggtca cctacctaat ctcgaggttc
     3421 ttgatttatc aaataatcaa ctgacaggtt tgccaaatga gttgggacaa ttaacaaagc
     3481 tcaaggttct taacctttca ggtaacgatt attctgttca agatcttgat attataataa
     3541 aaggtcttcc ttctgatgtg aatattattg ttgattaaga tctaatatcc acatattcaa
     3601 atttaaagat atgtattgac aggtatgcga gaagtgttat aagtaccata aaatcttaag
     3661 aaaggagata agaaatggca cccatgacaa aagaacgatt tttccaaatc gcaagagaat
     3721 atggtttatc tgatgaagta attcaatccc aatgggacac acgcccagct gataatattg
     3781 acgaggagaa attacgcaag gcaatatcaa aatacaatga ggttgaacag gaacgtcgtg
     3841 tgcatcaggc taccgtgtca attctgaaaa gtcacggaat attgtaagat agtttgccta
     3901 agacggtgta taaaaatatg ccgtcttttt gtttgattta taacttgtat aagaattaaa
     3961 ataatcttat agatataaat aacctataga agataactga agtatttttt caaatataaa
     4021 cgaagatttt tacgatggtt gagaacgtta gtccatgatt ttagttatct cgtaagttgc
     4081 cctgtgttta aattctgcac ctgattgtta gaacatcctc tgtccatcgc aatcttttat
     4141 gtggatcatt tattgaggtc aggctatctc tgagatttat caattgcttt ttaaacattt
     4201 tatccttctc gtctaaagaa gttttatcaa aatcgtttat tgcgtgggta agaaactcat
     4261 taaccgactc ttgaggtatt gggttattta gattgcccgc tgctgtcacc acccttaaaa
     4321 ggtccatttt tattgaattt ataaaaaatt gcttatctct cattttaaat ttctagaaat
     4381 tctttagtta aattttctaa acgctgtctt acttttggat ctgtgatttc ataggtgttt
     4441 tttcccaagg ctggtttgta attactcatt gccatatatt ccacttcctt agtgcttacg
     4501 cggtaacctc ctccaaagag attttctagt ctactaccac ttttaattag aacttcttcg
     4561 cagtcaatat gatattctcc accaatagct agtacatttt tttctatatc tataactgtt
     4621 ttgatgtagt cagagtatgc ttcacaagct tgtttgaatt gttcgtgagt tattttctta
     4681 tcaagtacta ttaccatcaa ttaatattat acatcatggt tttttaaggt taatttcaat
     4741 atgtaatctg tggctggggg acagggattc ggtcaccttc gcatctgcgg atgctcagtc
     4801 gaccccgctt tggcaccgtc gtgcctcagc attttcatcc ctgccccaac aaaaacaacc
     4861 acaaaaccta aaggccttgt ggctgttttc gtggctgggg gacaggga
//
LOCUS       DAOTFA010000036         4832 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate AMR_MDS_3115
            NODE_12738_length_4832_cov_7.125393, whole genome shotgun sequence.
ACCESSION   DAOTFA010000036 DAOTFA010000000
VERSION     DAOTFA010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868139
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (wastewater metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4832)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4832)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:36:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 536
            CDSs (total)                      :: 521
            Genes (coding)                    :: 520
            CDSs (with protein)               :: 520
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4832
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12738_length_4832_cov_7.125393"
                     /isolate="AMR_MDS_3115"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..1814)
                     /locus_tag="P5014_01765"
     CDS             complement(84..1814)
                     /locus_tag="P5014_01765"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HRY56870.1"
                     /translation="MNYEMRKNDENTQENTHKPVLKVIMIGGTEKVNKNLTVYEYGDD
                     MVVVDCGIGFPDIFDMPGVDTLIPDFDYLIKNSHKIKGVFITHGHEDHVGAVPNLLME
                     LPNVPIYASKLVGEFLKVKLSEKSFSNIGKNFSYHLFGPESGVVNLGHFKMSAFRINH
                     SIPESMGLVINTPEGVLVHLADYKFDENPVLDKPADVDTMERLGREGVLCLISDCLGI
                     TSEGHTKSESTLANTFHELFARAGNRQILVTTMSSNISRMYQIMDAAVKLNRKIVPSG
                     RSIEQMIDVGRRLGYLPFPDDFFINEKDSTSHDQKDLVYLIAGCYGQPGSSLERLSRD
                     EHESIVLQNDAMVIFSGDPNPPGADITVEKVRDNLTLKNSEVIYSEIQDNLHVSGHSV
                     RGDIERLAKLINPKYHVPIGGTITKMRAYKNVMFNMGIPKENVFECLEGDSVEFSNGR
                     AKKGKHYEIKQVYISSNKVDELNPIVVKDRSHLSSDGVFVVAIPASKEGDIFADKLEI
                     ITRGFIYVKDSKDLMDKSKKFIFKRIHNSKAGKKDPSDYKRRLENDISRFLKKETGMY
                     PMVIVHFITM"
     gene            complement(1860..>2272)
                     /locus_tag="P5014_01770"
     CDS             complement(1860..>2272)
                     /locus_tag="P5014_01770"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: protein motif:HMM:NF015146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HRY56871.1"
                     /translation="EDVWIGNIIKHRPPENREPLPDEIQACSPFLDMQLQIISPKIIV
                     TLGRYSMNHFYPNGKISIDRGNLVKAGKYYIYPIYHPAAALRNGSMMDDFKKDFKKIP
                     EMLIKVEDFLQKSSFEENGQNNPKSIDGQLTIGL"
     assembly_gap    2273..2372
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2373..2656)
                     /locus_tag="P5014_01775"
     CDS             complement(<2373..2656)
                     /locus_tag="P5014_01775"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012941094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HRY56872.1"
                     /translation="MDKEKLLKDISVSIKSCDKCRLCKKATNAVPGEGNVNSKIVFIG
                     EAPGYYEDISGHPFVGRAGKLLEESLKEIGYKREDVWIGNIIKHRPPENR"
     gene            complement(2660..3721)
                     /locus_tag="P5014_01780"
     CDS             complement(2660..3721)
                     /locus_tag="P5014_01780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRY56873.1"
                     /translation="MEKKDFLFEELNTLSKKIKDTKGMPEDLVTRLVQMLDRLDRMAN
                     LGHYATEFDTLSRYIETVTSIPWVEETKDILDLEGAKESMDRHHYGMQYAKDRILEYL
                     STLILLSQRGEGAVSKSPVLLLVGLQGVGKTTLAYSLAETLGRKFERIAMGGIGSSTE
                     LRGRSKSFPEAEPGQIIKAITRTGVRNPLILLDEIDKASGEQGLRSDIMAILLEILDP
                     KQNVAFRDHYVDYPIDLSNVLFICSANTTGTLSAALMDRMEIIKMPSYTDQEKTVIAK
                     EYLLPQIIQKSGLKPEELQIDPDLWPGIVRPFGYDSGIRSLGRTLESICRKVAREIVE
                     GKVTEVKLTSENLKYYLPQ"
     gene            complement(3721..4710)
                     /locus_tag="P5014_01785"
     CDS             complement(3721..4710)
                     /locus_tag="P5014_01785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56874.1"
                     /translation="MKRICPFCKSELTASSHHFCEYCGSELPEELYLKPKETVSEKIA
                     FSNNEGVKISKKPSKKDTTISKISHGISIRSVVGGVLLGMSISTMIFLFIQTDFLKVF
                     SSKINLKKPRIVSVEKPTTKTDTTEPANSTETKNKENNKNLGLDLRSGVFGQNNVYSY
                     IPYDFSLYVEISDPKTFDPYFSFLGGEFFTLAESLKGKINSPYSAFYMKKGLSSGWVL
                     IAFPADTSLEFLGNKDITTDKEGDALIISYEPELIDQVKLAKSEIEKNMSIHPSFVSI
                     KNILPKEGQVFLLKKQGDGDIVLNETMRNTLSEEFKSILTSFKESGDSYLVIK"
BASE COUNT         1419 a          965 c          698 g         1650 t
ORIGIN      
        1 taatttgaac tttttctttc tacctctgga cattatccct ataatagcac aaagaaggcc
       61 ttagtaaaaa ggctaactac aatttacatt gttataaaat gaacaattac catcggatac
      121 attccggttt cttttttaag gaatctggat atatcatttt ccaagcgtct tttgtagtct
      181 gaggggtctt ttttaccggc ttttgaatta tgaattcttt tgaatataaa ttttttggat
      241 ttatccatca aatccttaga atcttttacg taaataaacc ctctggtaat tatttccagt
      301 ttatccgcaa atatatcacc ctcttttgat gcaggtatcg ctacaacaaa taccccatca
      361 gatgataaat gacttctatc cttgaccaca attggattaa gctcgtcgac tttattgcta
      421 ctaatgtaaa cctgcttaat ttcataatgt tttccttttt ttgctcttcc atttgaaaat
      481 tcaacagaat caccttccaa acattcgaat acattttctt taggtatacc catattaaac
      541 attacatttt tgtaagccct catttttgtt attgttccac ctataggaac gtgatattta
      601 ggattaatta gcttcgccaa cctctctatg tcccctctaa cagagtgacc tgacacatga
      661 aggttgtctt gaatttcact gtaaataacc tcactatttt ttaaagttaa gttgtctctt
      721 accttttcga cggtaatatc agctcccgga ggatttggat ctccagaaaa tatgaccata
      781 gcatcatttt gaagaacaat tgactcgtgc tcatccctgc ttaatctttc taacgaagaa
      841 ccgggttgac cgtaacaacc tgctattaaa taaacaaggt ctttttggtc atggctggta
      901 gagtcttttt catttataaa gaagtcgtca ggaaatggaa ggtagcctaa ccttctcccc
      961 acatcaatca tctgttcaat agatcttcca ctgggaacta tttttctatt taatttcaca
     1021 gcagcatcca taatctgata cattctggaa atatttgaag acattgttgt caccaaaatc
     1081 tgacgatttc ccgccctggc aaataactca tggaaagtat ttgctaaagt gctttctgat
     1141 tttgtatgac cttcggatgt gattcctaaa caatcggaaa ttagacacaa aacaccctct
     1201 cttcctaatc tttccatagt gtctacatca gcaggtttat ctaaaaccgg attttcatca
     1261 aatttataat cagcaagatg tactaaaaca ccttccggag tatttataac cagacccatt
     1321 gattcaggaa tagagtgatt aattctaaaa gcagacattt taaagtgacc caaatttact
     1381 acccctgatt cagggccaaa aagatgataa ctaaaatttt ttccgatatt gctaaaagat
     1441 ttttcagaga gttttacttt taaaaattca ccgactaatt tacttgcgta aattggtaca
     1501 ttgggaagtt ccatcagcaa attaggcacc gccccaacgt ggtcctcatg accgtgagta
     1561 ataaaaacac ctttaatttt atgtgaattt tttattaaat aatcaaaatc ggggattaga
     1621 gtatcaactc cgggcatatc aaaaatatca ggaaaaccta tcccgcaatc caccactacc
     1681 atgtcatcac cgtattcata aacggtaaga tttttattta ctttttctgt cccacctatc
     1741 attattactt tcaaaacagg tttgtgggta ttttcctgtg tattttcatc attctttctc
     1801 atttcataat tcatatttgt ttatagttcc tttaatttct tttaagtaac caattaattt
     1861 tacagaccta tggtcaattg tccatcgata ctcttagggt tgttttgacc gttttcttca
     1921 aaactacttt tctggagaaa atcttcaact ttaatgagca tctccggtat ttttttaaaa
     1981 tcttttttaa aatcatccat cattgaaccg ttccttaaag ctgctgcggg atgataaatc
     2041 ggatagatat agtatttccc cgcctttact aaatttcctc tatcaatgga tattttcccg
     2101 ttagggtaaa aatggttcat agaataccta cccaaagtca caataatttt aggagaaatt
     2161 atttgcaact gcatatccaa aaaaggggag caagcctgga tctcatccgg aagaggctct
     2221 ctattttcag gaggacggtg tttaatgata tttcctatcc acacatcttc tcnnnnnnnn
     2281 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nntctctatt ttcaggagga cggtgtttaa
     2401 tgatatttcc tatccacaca tcttctctct tataacctat ttcctttaaa gactcttcca
     2461 ataatttccc agctcttcca acaaaagggt gcccggaaat atcttcatag taaccgggag
     2521 cttctccaat aaaaacaatt tttgagttaa cattaccttc gccggggacc gcgtttgtgg
     2581 cttttttgca caatctacat ttatcacagg attttatgct taccgatata tcttttaata
     2641 acttttcttt atccattaat tattgaggaa gataatattt caaattctcg gaggtgagtt
     2701 taacttccgt cacctttcct tctacaattt ccctagcaac ttttctgcaa atagactcaa
     2761 gcgtccttcc taaacttcgg ataccggaat cataaccaaa aggtctcacg attccaggcc
     2821 ataagtcagg atcaatctgc agttcttcgg gttttagtcc ggatttttgg ataatctgag
     2881 gtaataaata ttcttttgca attacagtct tttcttgatc ggtatatgaa ggcatcttta
     2941 taatttccat tctatccatg agcgccgcag aaagggtacc cgtggtattt gccgaacata
     3001 taaaaagcac attcgacaag tctatcggat aatcaacata atgatctctg aaagctacgt
     3061 tttgtttcgg atccaaaatt tccaataaaa ttgccataat atcggatctt aaaccttgct
     3121 caccgcttgc tttatcaatt tcatccaata gaataagagg atttctaaca cctgttctag
     3181 ttatagcttt tattatttga ccgggttcag cctctggaaa agatttgctt cggcctctta
     3241 actcagttga agagccgata cctcccatag caattctttc aaactttcta ccaagtgttt
     3301 cagctaatga ataagctaag gttgtcttac caactccttg aaggcccacc aataaaagta
     3361 cgggagattt tgacaccgca ccctcacctc tttgtgataa taaaattaaa gtagacagat
     3421 actccaatat cctatcttta gcgtactgca taccatagtg atgtctatcc atactttctt
     3481 tagcaccttc taaatctaaa atatccttag tctcttccac ccaagggatt gatgtgacag
     3541 tctcaatgta tctggataag gtatcgaatt cagttgcgta atgacccaga tttgccattc
     3601 tatctaacct gtctaacatc tgcactaatc tggttaccaa atcttcgggc attcctttag
     3661 tgtccttaat ttttttagat aatgtattta attcttcaaa taaaaagtct tttttctcca
     3721 ttattttatt accaaataag aatctccgga ttctttaaaa ctagttaaaa tagatttaaa
     3781 ttcttcagaa agagtgtttc tcatggtttc atttaataca atatcgccgt ctccctgctt
     3841 tttcaataaa aacacctgac cttctttagg aaggatattt tttatcgaca caaatgaagg
     3901 gtgtatggac atattttttt caatttctga ttttgccaac ttaacttgat ctataagttc
     3961 aggttcgtat gaaataatca aagcatctcc ttctttatca gttgtaatat ccttattacc
     4021 tagaaactca agagaagtat ctgctggaaa ggcaatcaaa acccacccac tcgacaaacc
     4081 ttttttcata taaaaagcag agtacggaga gttaatttta ccttttagac tttcagccaa
     4141 ggtaaaaaac tcaccgccga ggaaactaaa ataagggtca aatgttttag gatcagagat
     4201 ttctacatat aaagaaaagt cataaggaat gtaactgtaa acattatttt gaccgaatac
     4261 tcctgatcgt agatccaaac ctaaattttt attattttct ttatttttag tttcggttga
     4321 atttgcaggc tctgtggtat cagttttagt ggtgggtttt tcaacactaa ctatcctagg
     4381 tttttttaaa tttatcttac ttgagaatac ttttaaaaaa tctgtttgaa taaacagaaa
     4441 aatcatagtg ctgatagaca tacccaacaa aactcctcca accacgcttc tgatactaat
     4501 tccatgggat attttactaa tcgtggtgtc tttcttcgaa ggtttcttcg aaatttttac
     4561 accctcatta ttagaaaaag ctatcttttc ggagacggtt tctttgggtt taaggtagag
     4621 ttcttctgga agttcactcc cgcaatactc acaaaaatga tgactgctgg cagtcaattc
     4681 tgatttacaa aacgggcaga ttcttttcat ctcatttatt ataaagcaga tattaacttg
     4741 tttccagaat ttttaacgat tagataagaa cacaagaatt atcttcttct atctcttctg
     4801 ttatcatttc tgaagttacc gcccctgcct cc
//
LOCUS       DAOTFA010000037         4727 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate AMR_MDS_3115
            NODE_13145_length_4727_cov_8.170805, whole genome shotgun sequence.
ACCESSION   DAOTFA010000037 DAOTFA010000000
VERSION     DAOTFA010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868139
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (wastewater metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:36:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 536
            CDSs (total)                      :: 521
            Genes (coding)                    :: 520
            CDSs (with protein)               :: 520
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4727
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13145_length_4727_cov_8.170805"
                     /isolate="AMR_MDS_3115"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..995)
                     /gene="argS"
                     /locus_tag="P5014_01790"
     CDS             complement(<1..995)
                     /gene="argS"
                     /locus_tag="P5014_01790"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HRY56875.1"
                     /translation="MDYVSSTLNENIKKSIKNIYDLDVDSVPLEHPENEKFGDYSTSV
                     SLVISKKLKKIPLDIAKEISKDLNHKDIYFENNYGKYPLFSSIEAVSPGFINFKFSDL
                     FLKNQITEVLDKGDSFGSNISGEGKIIIIEFSAPNPNKPLHIGHSRNNFLGSSLSELF
                     LFSGYNVIRMNYMNDWGTHICKSMLMYKKYGEGKEPDKKPDHFVGDFYRMYEVEHEKD
                     PQGLDTELSDMFKKMESRDKETLNLWKKITGWAYEGWKSTYENENVVFDRLEYQSNYT
                     RAGKEIIDLALKEGVAIKDSTGAVVAKLEEYGIPDKVLLRSDGTPIYITQDIQMA"
     gene            complement(1107..1931)
                     /locus_tag="P5014_01795"
     CDS             complement(1107..1931)
                     /locus_tag="P5014_01795"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRY56876.1"
                     /translation="MKRSDVILVVLVYLLFSFRGVASAGDGYLNLEILDVGQGDSILI
                     TTPNGKRVLVDGGDNYESDYEISKRIPFYSCYIDILILTHPHYDHIVGLNRIMQRCKI
                     GTIMFNDVSFDSRDFRLFKELSSNFNIKNMYAGDIFEIDEVNFIFLWPYKKFLQNRIP
                     DLNDMSFVFLVDYGKFEALLTGDATEKVLGRLSFNKINELTDSSLDVLKVPHHGSKYS
                     LHKYFYSGIDPKMCVISVGKDNKFSHPYPGLVEYLNEIGCNVLRTDESGDVLIRVR"
     gene            complement(1918..2898)
                     /locus_tag="P5014_01800"
     CDS             complement(1918..2898)
                     /locus_tag="P5014_01800"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HRY56877.1"
                     /translation="MGSVKDACLEKRESIFSDKSKFPGKQISKFRERSINISRKYLPS
                     PHSELLIGMVIGADELEKIPAFKEALKTTGTIHVVVVSGFNVSLVASLIMSVLGSKYK
                     MRNLLISQLFTFSYSVMTGLEPPVIRAWIMGGVVSLVKYYGRDINGFTALLFTALVMI
                     IIWPYFLLSVSFQLSFLATLGLIVFGNGIVEGLRKLFKTEAFFIEDLGTTISAQVFVL
                     PIVSYYFGRVSIISFLVNPLVLWTVPIATVIGTFFMFVAGLSSVVAKIMSIVMYPFLN
                     IFTVVVNYFSRFSFSSINFVLGIVPVIIYYFLVILLIFFINKKRRKSEEK"
     gene            complement(2986..3741)
                     /locus_tag="P5014_01805"
     CDS             complement(2986..3741)
                     /locus_tag="P5014_01805"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HRY56878.1"
                     /translation="MGLKYFENFSNIVKSNLIICFLFSSVIGGLVVYLSLQKRIQSNV
                     VVEVSADNTDKSSDNEESEDTNLYIDVSGAVIKPGVYEVKQGTRVGELIATAGGASDD
                     ASSMWVSRNLNLSKKLEDSTKIYIPFSWEFYFPEEYKISKLVNENYSADTDFDQDDSN
                     KNDSDDDNSADETDISDGGAGDTSDSGGLEDDGKINVNKATSSELDTLPGIGPAYAEK
                     IISNRPYKDIAEFESKSGLYKSTIENIKELITF"
     gene            complement(4077..4370)
                     /locus_tag="P5014_01810"
     CDS             complement(4077..4370)
                     /locus_tag="P5014_01810"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRY56879.1"
                     /translation="MRLEKLSKALSDKNRLQILEFLSDGQRNVSDVADRLNVEENLAS
                     HHLRVLASLGFLKNDKKGREVYYMLNEAKIVALLRDLRRNSRFKSILQESLRD"
     gene            complement(4420..>4727)
                     /locus_tag="P5014_01815"
     CDS             complement(4420..>4727)
                     /locus_tag="P5014_01815"
                     /inference="COORDINATES: protein motif:HMM:NF024731.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium chelatase"
                     /protein_id="HRY56880.1"
                     /translation="ETLDFIKNKVANTRLIQQKRFKDEKIFTNSEIANSQIDKFCSFG
                     EGAKRLLEQAIDAYCLSARAYFKIIRVARTIADLDEKSDIEINHIAEAVQYRSKFFL"
BASE COUNT         1576 a          910 c          640 g         1601 t
ORIGIN      
        1 ttagccattt ggatatcttg tgtaatgtaa atgggagtac catcacttct taaaagaact
       61 ttatctggaa ttccatattc ttccaacttt gctaccaccg ccccagtcga atctttaata
      121 gccacgcctt cttttaacgc caaatcaatt atctccttcc ccgctctggt gtaattactt
      181 tgatattcaa gcctatcaaa aacaacattt tcattttcgt aagtagattt ccaaccctcg
      241 taagcccaac ctgttatttt tttccaaaga tttaatgttt ctttatctct actctccatt
      301 tttttaaaca tatcagaaag ctctgtgtcc aaaccctgtg gatctttttc atgttcaact
      361 tcatacatcc tataaaaatc gccaacaaaa tgatcaggtt ttttatcagg ttctttacct
      421 tcaccgtatt ttttgtacat caacatagat ttacaaatgt gggtacccca atcgttcatg
      481 tagttcattc ttattacgtt gtaaccacta aataaaaata attccgaaag agagcttccc
      541 aaaaaattat ttctggaatg acctatgtga agcggcttgt tagggttagg ggcagaaaat
      601 tcaataataa ttatcttccc ttcaccgcta atattagacc caaaggaatc ccctttatcc
      661 agtacttcag taatttgatt ttttaaaaac aggtctgaaa atttaaaatt tataaatccc
      721 ggagaaacag cctcgataga tgaaaaaaga ggatactttc cgtaattgtt ttcaaaataa
      781 atatctttgt gatttaaatc ttttgatatt tctttggcga tgtctaatgg aattttttta
      841 agttttttag aaattactaa gcttacactg gttgagtagt ccccgaattt ttcattttcc
      901 ggatgctcca gaggaacact atcaacatcc aaatcataaa tatttttgat agattttttt
      961 atattttcat ttaaagtaga ggagacgtaa tccataataa acccctgttt ttctatatta
     1021 tcacgaataa aattcaaaat cccttctgta aaatactccg aaaaaaaata acccgatatt
     1081 taccagcgct ctacccagcg aggacgtcac ctaactctta ttaaaacatc tccggactca
     1141 tcggttctta aaacgttaca tccaatctcg ttgagatact ctacaagccc gggatagggg
     1201 tggctaaact tattatcttt acctacagat attacacaca ttttggggtc tatcccagaa
     1261 tagaaatact tatgtaaact gtacttggat ccgtgatggg gcaccttcaa aacatccaaa
     1321 ctgctatctg ttaactcatt tattttatta aaagacaacc gacctaaaac tttctcggta
     1381 gcatctccgg ttaacaaagc ttcaaatttt ccgtaatcca ccagaaatac aaaggacata
     1441 tcgtttaaat cgggaattct attttgcaga aatttcttat aaggccataa aaaaataaaa
     1501 ttaacttcat caatttcaaa aatatcccca gcgtacatat tttttatatt aaaattagat
     1561 gaaagttcct taaataacct aaagtcccta gagtcgaagg agacgtcgtt aaacattatg
     1621 gtaccgattt tgcacctctg cataattcta tttaaaccaa caatgtgatc gtaatgtggg
     1681 tgagttagga taagaatatc tatataacaa gaataaaaag gtattctttt tgatatttcg
     1741 taatcagatt cgtaattgtc tcccccatca actagaactc gcttaccgtt cggagttgtt
     1801 attaaaatag aatcaccttg tccgacatct aaaatttcta ggtttaagta tccatctccc
     1861 gccgaagcaa cacctctaaa agaaaataaa agatagacta aaactacaag aattacatca
     1921 cttctcttca ctttttctcc tttttttatt tataaaaaaa attaaaagaa ttaccaaaaa
     1981 gtaatagata atcacgggca ctatgcctaa tacaaaatta atacttgaaa agctaaaacg
     2041 tgaaaagtaa tttacgacaa cagtgaaaat atttaaaaag ggatacatta caatactcat
     2101 tatttttgct acaacacttg ataaaccggc aacaaacata aaaaatgtac ctattacggt
     2161 ggcaatgggt acagtccaaa gaaccaaagg attaactaaa aaacttatta tgctaactct
     2221 tccaaaataa tacgaaacta tagggagtac aaaaacctga gctgaaattg ttgttcccag
     2281 atcttcaatg aaaaaggctt cggttttaaa taattttcga agcccttcta caataccgtt
     2341 tccaaaaact attaaaccta aagttgctaa aaaacttaac tgaaaactca cacttaataa
     2401 aaaatatggc caaataataa tcataactaa agctgtaaat aaaagagcag taaatccgtt
     2461 gatatccctg ccatagtact tcaccagaga tacaacacca cccattatcc aagctcttat
     2521 aactgggggt tcaagacccg tcattactga atatgaaaaa gtaaacaatt gagagatcag
     2581 aagattacgc attttatatt ttgatcctaa aacactcatg attaaactgg caactaaact
     2641 aacgttaaat cccgatacta caacaacatg aatagtacct gttgttttta aggcttcttt
     2701 aaaagcaggt attttttcca gctcatcagc tcctattacc atacctatta aaagctcgct
     2761 gtgaggactt ggaagatatt ttctggatat gttaattgac ctttctctaa attttgagat
     2821 ctgctttccg ggaaatttgc tcttgtctga aaatattgat tctctttttt ccaaacaagc
     2881 atcttttaca gaacccatac agttaaaata gactcttaga caagccacaa taataattaa
     2941 aagtaagggg aggtaatatt ttaaaatctt aaacatttga gattactaaa aagtaataag
     3001 ttctttaatg ttttcaattg ttgatttata caaacctgac ttggattcaa attccgctat
     3061 atctttatat ggtctgttag aaattatttt ttctgcgtag gctggaccaa tgcccgggag
     3121 tgtgtccagc tcagaagatg ttgctttatt tacattaatt ttcccgtcat cttcaagacc
     3181 cccagaatcc gaagtatccc cagctccccc atcggatata tctgtttcat cagcagaatt
     3241 atcgtcatca ctgtcatttt tattgctgtc atcttggtca aaatcagtat cagcagagta
     3301 attttcattt actagttttg atattttgta ctcctcggga aaataaaatt cccacgagaa
     3361 agggatataa atttttgtag agtcctctaa tttttttgat aaatttaaat ttctggaaac
     3421 ccacattgat gatgcatcat cggaagctcc tccagcagta gctatcaatt ccccaactct
     3481 tgtaccttgc tttacttcgt aaactcccgg tttaatgaca gctcctgaaa catctatata
     3541 taaattagta tcctctgatt cttcattatc agaggattta tcggtattgt ctgcagatac
     3601 ttcaacaaca acatttgact gtattctttt ctgcaaagat aaataaacca ccaatccgcc
     3661 tattacagat gaaaataaaa agcaaataat taaattactt ttaacaatat tggaaaaatt
     3721 ttcaaaatat ttaagtccca ccactgaata taatcattat gaaaggagtt tgtttataca
     3781 cctctctcta ttattaatag agtttattac atttgttggt ttcttagtct ttaaatagat
     3841 ggatgatttg atttatttta attatttaac gcactattac cgcagttttt acgccttgat
     3901 attgttaagg ccttatttat tattattatt tatttataaa attaaatagt gaatattaat
     3961 actaattata taccgcaaat aaaacgcaat tgataccttt tgaaatattt ccccacactc
     4021 ccataaaaca aacacaaacc aaattattgg gaaatataaa ttattttata ggtatcttaa
     4081 tctcttaaag attcctgaag tatgctcttg aatctggaat ttcttctcag atcccttaaa
     4141 agagctacta tttttgcctc gtttaacatg tagtagactt ctcttccctt tttgtcgttt
     4201 ttcaaaaaac ccagtgacgc caagactctc aaatgatgag aagcaagatt ttcctcaaca
     4261 ttaagtctgt cagcaacatc actaacgttt ctctgcccgt cgcttaggaa ctctaaaatt
     4321 tgtaacctat ttttgtcgga cagtgctttt gaaagttttt ccaacctcat ggcccgacta
     4381 tacaacactt gttgttgttt ttcaagtcct atgttttagc tacaaaaaaa acttagatcg
     4441 atattgtaca gcctccgcta tatgattaat ttcaatgtca cttttttcat ctaaatccgc
     4501 aatagttcta gcaactcgga ttattttaaa ataagctctt gctgaaagac agtaagcgtc
     4561 tattgcttgt tctaacaatc ttttagcccc ttcgccgaaa ctacaaaatt tatctatttg
     4621 agaatttgcg atttctgaat tagtaaatat tttttcatct ttgaaccttt tttgttgaat
     4681 aagtctggta tttgcaactt tattttttat aaaatccaag gtttcgt
//
LOCUS       DAOTFA010000038         4691 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate AMR_MDS_3115
            NODE_13288_length_4691_cov_7.605910, whole genome shotgun sequence.
ACCESSION   DAOTFA010000038 DAOTFA010000000
VERSION     DAOTFA010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868139
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (wastewater metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4691)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4691)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:36:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 536
            CDSs (total)                      :: 521
            Genes (coding)                    :: 520
            CDSs (with protein)               :: 520
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4691
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13288_length_4691_cov_7.605910"
                     /isolate="AMR_MDS_3115"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            26..481
                     /locus_tag="P5014_01820"
     CDS             26..481
                     /locus_tag="P5014_01820"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HRY56881.1"
                     /translation="MSDTIFDKIVKGETPCWKVWEDGKHMAFLTPFPNTPGVTVVIPK
                     ENKGDYVFSLSDEDYKDLLEASKTVAKILEKAFDSPRVAMVFEGTGVAYVHTKLYPLH
                     GELASQTNVWSKTRKFYPKYLGYLTTIEGPQMKDEDLAKIQHKILEAQK"
     gene            574..1734
                     /locus_tag="P5014_01825"
     CDS             574..1734
                     /locus_tag="P5014_01825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56882.1"
                     /translation="MSVEKSLSNNSSKKFSNKNILLALLGVIALIALIIYFQYKNRPK
                     STEESTYLIKEESGTNNKNTEPFSSIDIDGDGDKEQVPREIVESKILMLTENNQLYVI
                     SPIDKDKKLLIDGVSSYASSKDKNYIAYLKTYPFITDKAKSDGDIHIYNLKTQQESKI
                     VAGKAAQRNISWSPDGRYVIVERGTSTTGSNDVYSVETGKNTGCTFSGSILWISNTEF
                     LNPLFSNNFTPRGGQVINARGIRKVNIETCQSETLILPTNTADFSAIKLMGNDLVVLK
                     TYVDKPEDWTDFSQGSKIKTTYEKFNLLTKLGIPYPEYENEIKIETDRLKALVPYTVK
                     VKRIYTTNKDMATGWELINVYKGGTIYNNEVYLMGPEKTVVKIGDNATGTWL"
     gene            complement(1753..>2288)
                     /locus_tag="P5014_01830"
     CDS             complement(1753..>2288)
                     /locus_tag="P5014_01830"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF014796.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HRY56883.1"
                     /translation="EIHYSKHHQNYLNKLVEALDKHPELSEVPLENILSDLDIVPEDI
                     RTAVKNNGGGHFNHSFYWSIMNPNTKKIPSDEFLSKIGDFEKFKKDFTDKALGLFGSG
                     WVWLVLNEHGDLEILQTSNQDCPLSMGKKPLLPIDLWEHAYYLKYQNRRSEYIEAWWN
                     LVDWEVVEKIFEKYNSR"
     assembly_gap    2289..2388
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<2389..2513)
                     /locus_tag="P5014_01835"
     CDS             complement(<2389..2513)
                     /locus_tag="P5014_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017449374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HRY56884.1"
                     /translation="MNKTLPKLNYNYDSLEPFIDAKTVEIHYSKHHQNYLNKLVEA"
     gene            2617..3273
                     /locus_tag="P5014_01840"
     CDS             2617..3273
                     /locus_tag="P5014_01840"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HRY56885.1"
                     /translation="MTYKLSPSDFAYLYEECKRCFYLKVKENIPRPSTPFPGVFTTLN
                     SMVQTKLIGKNLITLSQDLPDCTVVNQEVFVESKTIPNTSCYIKGKYDLLGKNPDGTY
                     TVVDLKISLPSEEKIEKYKTQLFSYKFALENPVSSTPIKITKMGLLIFYPEYVDFIDE
                     TSKVNFPPKWMEIPTDEKAFYNFIKEVDDLLEGPVPEMNPSCKFCEYSKIRNNYEVEY
                     "
     gene            3502..3576
                     /locus_tag="P5014_01845"
     tRNA            3502..3576
                     /locus_tag="P5014_01845"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3534..3536,aa:Thr,seq:cgt)
     gene            3646..3876
                     /locus_tag="P5014_01850"
     CDS             3646..3876
                     /locus_tag="P5014_01850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56886.1"
                     /translation="MPNENLSGLIISLQKEFRDFRKETNENFNEVYSKLDNHTASLIS
                     LENTVGFYGDMYKMNKDKIDNHEKRISVLEAK"
     gene            complement(3992..4333)
                     /locus_tag="P5014_01855"
     CDS             complement(3992..4333)
                     /locus_tag="P5014_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56887.1"
                     /translation="MGFSSIIPKIKGNQNFEIKDFSDCLWFFISYLLLVAIGFGPFDF
                     FVLVPENKVRIIIRTKKLTIKANANTFALGSISPALSNALNALIIIKTKTVIRPTLKI
                     FICTPFSTLGL"
BASE COUNT         1639 a          756 c          764 g         1432 t
ORIGIN      
        1 ctccagatga tacaatggaa ctaatatgtc tgacacaatt tttgataaaa tagtcaaagg
       61 cgaaacacct tgttggaaag tatgggaaga cggcaaacac atggcatttt taactccttt
      121 tcctaataca ccaggggtta cggttgttat accaaaagaa aacaaaggag attatgtttt
      181 ttctttaagt gatgaagact acaaagattt acttgaagca agtaaaacag tagcaaaaat
      241 acttgaaaag gctttcgatt cacccagggt agcaatggta tttgaaggaa caggcgttgc
      301 ctatgttcac actaaattat atcctctaca tggtgagcta gcctcacaaa caaatgtttg
      361 gagtaaaacc cgaaaattct atccgaaata tcttggttat ttaacaacta tagagggacc
      421 acaaatgaaa gatgaggatc ttgctaaaat tcaacataaa atcctagagg cacagaaata
      481 aacaatcatt tacagatccg actatcgctt cataatttca caatcttagt acccgtactt
      541 tattttaaaa attatttatt ctatacttct aatatgagtg ttgagaaatc cttatccaat
      601 aattcttcaa aaaagttttc taataaaaat attttattag cgttattggg cgtaattgcg
      661 ctaattgctc ttattattta ttttcaatat aagaatagac caaaatcaac tgaagaatct
      721 acttatttaa taaaagaaga atctgggact aataataaaa atacggaacc tttctctagt
      781 atcgatattg acggagatgg agataaggag caagttccta gagagatagt ggagtcaaaa
      841 attcttatgc ttacagagaa taaccagctt tacgtaatta gtcctataga taaagataaa
      901 aaacttctta ttgatggagt gagttcttac gcttcctcaa aagataaaaa ttatattgcg
      961 tatttaaaaa cctacccgtt tattactgac aaagcgaaat cggacggtga tatacatatt
     1021 tataatctta aaacacagca agaatctaaa attgtcgccg gtaaggctgc acaacgaaat
     1081 atttcgtggt ctcccgacgg aagatatgta attgttgaga gaggaactag taccacagga
     1141 tccaatgatg tttattctgt agaaacagga aaaaacacag gctgtacttt ttctggaagt
     1201 attttgtgga tcagcaatac tgaatttcta aatcctcttt tttcaaacaa tttcacccca
     1261 agaggaggac aggtaataaa tgcccgggga ataagaaaag ttaatatcga aacttgccaa
     1321 tcagaaacct tgatattacc aacaaataca gcagactttt cagcaattaa attgatgggt
     1381 aatgacttag tggtactaaa aacctatgtc gataaacctg aagattggac tgatttttct
     1441 caaggatcaa aaataaaaac tacttatgaa aaattcaacc tactgaccaa acttgggatt
     1501 ccttacccag agtacgaaaa tgaaattaag attgaaacag atagattaaa ggccttagta
     1561 ccctacacag taaaagtgaa aaggatttac acaactaaca aggacatggc cacgggatgg
     1621 gaattgataa atgtgtacaa gggtggcact atttacaata acgaggttta cctaatgggt
     1681 ccagaaaaaa ctgttgtaaa aatcggagat aacgctaccg gaacctggct ttaaatttca
     1741 actacagtag ccttaccttg aattatattt ttcaaatatt ttttctacaa cttcccaatc
     1801 aaccaaattc caccaagctt caatatactc cgatcttctg ttttgatatt tcaaataata
     1861 agcatgttcc caaagatcaa ttggtaaaag tggctttttt cccatactta aaggacaatc
     1921 ttgattgcta gtctgcaata tttctaaatc cccgtgttca tttaaaacca accaaaccca
     1981 accgctcccg aacaaaccca atgctttatc cgtaaaatct tttttaaatt tctcaaaatc
     2041 acctatttta cttaaaaact catccgaagg tatttttttt gtattaggat tcataataga
     2101 ccagtaaaaa gaatggttaa aatgaccacc tccgttgttt tttacggccg ttctgatatc
     2161 ttctggaaca atgtccaaat cacttaatat attctcaagg ggaacttcgc tgagttcggg
     2221 gtgtttatcc aaagcttcta caagtttatt taaataattt tggtgatgtt ttgagtaatg
     2281 tatctcaann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2341 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngc ttctacaagt
     2401 ttatttaaat aattttggtg atgttttgag taatgtatct caacagtttt agcatctata
     2461 aaaggctcta gagaatcgta attgtaattt agtttgggta atgttttatt catatttata
     2521 ttttacatta attaaacttt ttccgaaaac tgaagaatat tttcatattt caatgttaaa
     2581 ataagatatt tacaaacttc aatgttaaaa taagacatga cttataaatt atcaccttcg
     2641 gattttgcgt atttatacga ggaatgtaaa cgctgttttt acttaaaagt aaaagaaaat
     2701 atcccgagac cttctacccc attccccggc gtttttacaa ctctaaattc aatggttcaa
     2761 acaaaattaa taggaaaaaa tttaataaca ctttcgcagg atttacctga ctgcactgtg
     2821 gttaatcagg aagtatttgt ggaatcaaaa actattccaa acacatcttg ttatattaaa
     2881 ggaaaatacg accttttagg caaaaatcct gatggtactt acactgtagt cgatcttaaa
     2941 atatcactac cgtcagaaga gaaaattgaa aaatacaaga cccagctttt ttcatataaa
     3001 tttgcacttg aaaatcctgt ttcgagcaca cctataaaga ttacaaaaat ggggctactt
     3061 attttttatc ctgaatatgt tgattttatt gatgaaactt ctaaagttaa ttttcctcct
     3121 aagtggatgg agattcctac agatgaaaaa gctttttata attttataaa agaagttgat
     3181 gatttgctgg aaggtcccgt ccctgaaatg aacccttcgt gtaagttttg cgaatatagt
     3241 aaaatacgta ataattacga agttgagtat tgaagtaggg gtagattagg gtttaatgta
     3301 caaaatttat ttttatatgc cagagtagct caatgggaag aacctctcgc ttcgctcgag
     3361 aaccttgcga ttgagcttac taatatattt agaataattg tggtaaccaa agtaaaatta
     3421 attggactaa ggttattttt ataagccaga gtagctcaat ggtagatcaa gtgtatcgga
     3481 cgctaaagct agtatttttt tgccgaagtg gctcaatggt agagcaagtg tatcgtaaac
     3541 actaggttgt gggttcgatt cccaccttcg gctccaacac atttcctcta ttatcaatag
     3601 aaataccatc ttgttttcct aaaataaata aactaatatc caaacatgcc taatgaaaat
     3661 ttatcaggtt tgataatttc cctccaaaaa gaatttagag attttagaaa ggaaaccaat
     3721 gaaaatttta atgaggtata ctcaaaactg gacaaccaca cagcgtcact aatttcttta
     3781 gaaaatacag tgggattcta tggagatatg tataagatga acaaagataa aatagataat
     3841 cacgagaaaa gaatatctgt tttagaagca aagtagcaga cccccgaatt tcttttttaa
     3901 ttttcaactc ctttactata atattctcac ctgattttta gtgatttgaa gaaactcaga
     3961 ctggacaaat catgtactat gggttataat gctataaacc taaagtagaa aaaggagtac
     4021 agatgaaaat cttcaaagta ggtctaatta cggttttagt tttgataatt atcaaggcat
     4081 tcaaggcatt cgagagcgct ggtgagatgg accccaacgc aaacgtattt gcgtttgctt
     4141 ttatagtcag tttttttgtt ctaattatta tcctgacttt attttcagga actagaacaa
     4201 aaaaatcaaa tggtccgaaa cctatggcaa caagaaggag ataagaaatg aaaaaccaaa
     4261 ggcaatcaga gaaatctttg atttcaaaat tctggttgcc tttaattttt ggtataattg
     4321 atgaaaaccc cacaatataa aggagagacg atgagtagag atgtttgcga cactggtaaa
     4381 atagaagaga tgggtacttc gaatgatcct aaactcagaa aaagagtgaa actgttgatg
     4441 gtgattttcg ggttaggggc tttagcttat atcttatttg tagcatttgt gttttatatc
     4501 aagtagtaag gaatagaaac agacaacact ctttatttat aatgggggta gataataacc
     4561 aaaactaccc ccacaatttc taaccttttc catttatccc caattaccca aactcataaa
     4621 tctgataaaa taatcctatg aaaattaaaa aagctataat cgcagcagca ggtctaggta
     4681 ctagatttct c
//
LOCUS       DAOTFA010000039         4376 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate AMR_MDS_3115
            NODE_14659_length_4376_cov_6.963666, whole genome shotgun sequence.
ACCESSION   DAOTFA010000039 DAOTFA010000000
VERSION     DAOTFA010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868139
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (wastewater metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:36:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 536
            CDSs (total)                      :: 521
            Genes (coding)                    :: 520
            CDSs (with protein)               :: 520
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4376
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14659_length_4376_cov_6.963666"
                     /isolate="AMR_MDS_3115"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1364)
                     /locus_tag="P5014_01860"
     CDS             complement(<1..1364)
                     /locus_tag="P5014_01860"
                     /inference="COORDINATES: protein motif:HMM:NF023495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rnase Y domain-containing protein"
                     /protein_id="HRY56888.1"
                     /translation="MDSLNIIFIVMALLGLSGGYVFYSKKSKKEETPKVIENSQADSA
                     PKKFHETRIDVENDPVVMEAKAKSRELIVEAKAEALNIQTKAQDQARKIREDALETER
                     KSMVLKAQVDAKARELESKAKSLVSIKENLEKKQEEIENFHRKQQEEMDNFNRKQQEE
                     IEKIASMSKEEAREILLKNLDKSLVEDKAKRIKEKEEEIRRDSQEMSKQILMEALEFG
                     STDIVIEHSTSKIKLPDEDIKGRIIGKEGRNIRTFEELTGVDLDLDSSPGDIIVSCFD
                     SVRRAVATAALERLIADGRIQPARIEEIVEQTKKEMDNIMYKAGDSLCHKLGLYNIPK
                     ELIQTLGKFKYRFSYGQNMLEHTMETARIGMYIAEELGADIDTVRLGCVFHDIGKVLT
                     DQDGSHVDLGVELLRKFKIPEEAIKCVAEHHDDRQTTIESAIVTLADHVSGARPGSRG
                     EDYES"
     assembly_gap    1700..1799
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1800..2211)
                     /locus_tag="P5014_01865"
     CDS             complement(<1800..2211)
                     /locus_tag="P5014_01865"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecX family transcriptional regulator"
                     /protein_id="HRY56889.1"
                     /translation="MDLNNLDKAYSRVYFKVLNFLSFSKRSESEIFGKIQTYILKTRI
                     SPKEKEILKDRVITNLKSDGYLKESNDQDYAYSYIQGLKNSGKSFNKIKVLKFLLKKG
                     ISKDIIDESLSEIEDDDIYESVLKEAEKKIKYIKG"
     gene            complement(2223..3335)
                     /gene="recA"
                     /locus_tag="P5014_01870"
     CDS             complement(2223..3335)
                     /gene="recA"
                     /locus_tag="P5014_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HRY56890.1"
                     /translation="MAAKTSSKLKEIKEKSPAQAGKDLALKTAIDQIEKNFGKGSIMK
                     LGGSVGERTIPVIPTGSIALDMALGVGGIPRGRIIEIFGPESSGKTTLVQHIIAETQK
                     LGGTAALIDAENAFDPSYAEKTGVDVENLLVSQPDTGEQALDIAETLIRSNAVDLVAI
                     DSVAALVPRAEIEGDIGDSHVAMQARLMSQALRRLSGAISRSKCTVVFTNQLRSMIGV
                     MFGNPETTAGGKALKYYASIRIDIRKIGTLKRGEEFYGIRAKAKVVKNKVAPPFRSAE
                     FDILFNEGISKEGNLIDVGVDLGVVKKSGAWLEFNGEKIGQGKDAARMYIKENPKISA
                     EIEKGIRNSIKEKSDIPLNIGTKETEKASIEETSEE"
     gene            complement(3464..>4376)
                     /locus_tag="P5014_01875"
     CDS             complement(3464..>4376)
                     /locus_tag="P5014_01875"
                     /inference="COORDINATES: protein motif:HMM:NF012951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate--tRNA ligase family protein"
                     /protein_id="HRY56891.1"
                     /translation="DDQVLIKSDGFPTYHLAVVVDDHLMNITHVLRGREWLPSTPKHV
                     LLYEFFGWEKPKFIHLPLLRETDSTKKLSKRTGSVSALGFLQEGYLPEALLNFMMFLG
                     WNPGTEREVYSLEDFVKDFSIEKIQKSEMAAFDRQKLLWFNGLYIRNLPLEDLWNRVL
                     KWHEEYSEKLNLKNSTDARNLKILEIVRERMKKLSEFNDITFYFFEEPKIDKKTAEKF
                     SGDSKKTHEIIDNFIEVYKNIPSEKWNVPDLDAISHEKLAEFSYKPKEAFMTIRYITT
                     GCESTPPLFDVLGLLGKEETLKRLKKF"
BASE COUNT         1183 a          880 c          601 g         1612 t
ORIGIN      
        1 gattcataat cctcacctct ggaaccgggc ctcgctccac taacgtggtc agcaagtgta
       61 acgatggctg attcaatggt tgtctgtctg tcatcatggt gctcggcaac acattttatt
      121 gcctcttcgg gaattttaaa ttttctaaga agttcaacac ccagatcaac gtgagaaccg
      181 tcttggtcag ttaatacttt tcctatatcg tgaaacacac atccgagtct aactgtgtct
      241 atatctgccc caagctcttc cgcaatgtac ataccaattc tggctgtctc catagtgtgt
      301 tcaagcatat tctgaccata agagaaccta tatttaaatt tccctaacgt ctgaattagt
      361 tctttaggga tattatataa acctaattta tggcataaag aatctccggc cttatacata
      421 atgttgtcca tctctttttt agtctgctca acaatttctt caattcttgc aggctgaatt
      481 cttccatctg caattagtct ctccaaagcc gcggtagcaa ctgctcttct aactgaatca
      541 aaacatgaaa caattatgtc cccgggtgaa gaatccaaat caagatcaac tcctgttaat
      601 tcttcaaatg ttctaatatt tctcccctct tttcctataa ttcttccttt tatatcttca
      661 tccggaagtt taatttttga ggtggagtgt tctattacaa tatccgtaga accaaactcc
      721 agcgcctcca tcaagatttg ttttgacatt tcctgagaat ctcttcgaat ttcttcttct
      781 ttttctttta ttctttttgc tttgtcttca actaagctct tatccaaatt tttaagcaaa
      841 atttcacgag cctcttcctt agacatagag gctatttttt caatttcctc ctgttgtttc
      901 ctgttaaaat tatccatttc ttcttgttgt tttctatgaa aattctcgat ttcctcctgc
      961 tttttttcta agttttcttt tattgaaaca agactttttg cttttgattc aagttctctt
     1021 gccttagcat caacttgcgc ttttaacacc atagattttc tttcggtctc aagagcatct
     1081 tccctgattt tccttgcctg atcttgagct ttagtttgta tatttaaagc ttcggctttc
     1141 gcctctacaa tcaattctct ggatttggct tttgcttcca taacaacagg atcattctct
     1201 acatctatcc ttgtttcatg gaatttcttt ggtgcagaat ctgcctgtga attttctatt
     1261 actttcgggg tttcttcctt tttacttttc ttggaataga aaacataacc ccctgataga
     1321 cccaaaaggg ccattactat aaatataata tttagtgaat ccatttttta tttcctccat
     1381 atatcaacaa taaatccaac ctattacaca aggttttaaa ttttatggga attgctttaa
     1441 aaagggttta cacccgataa atatcgttat aacaacaaaa ggtattcata tttgtaaacg
     1501 aaaaatcttt tttcatagca aattccacag ggtaattcta ttgcaagtcg gttaaagtgt
     1561 caattacgga agatacggtt tcataggggt agccttttct caaaagaaag gtcgttaatt
     1621 tttgcttttt ttgaaaattg ttttcacctt taatatattt aatcttcttt tcagcttctt
     1681 tcaaaacact ttcataaatn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc
     1801 acctttaata tatttaatct tcttttcagc ttctttcaaa acactttcat aaatgtcatc
     1861 atcttcaatt tctgaaagag attcatcaat aatatctttt gagattcctt tttttaataa
     1921 aaattttaaa acctttattt tattgaaaga tttacctgag ttttttaacc cttgaatata
     1981 agaataggca taatcttggt catttgattc cttcaaataa ccatcacttt taagatttgt
     2041 aattacacgg tcttttaata tctctttttc ttttggagat atgcgtgttt ttaaaatata
     2101 agtttgaatt tttccaaaga tctcgctttc acttctctta ctaaaagata aaaaattcaa
     2161 tactttgaaa tatacccttg agtatgcttt atctaagtta tttaaatcca ttatttaata
     2221 ttttattctt ccgaagtctc ttctatcgaa gctttctcgg tttcttttgt ccctatgttt
     2281 aaaggtatat cgctcttttc tttaatagaa tttcttattc ccttttctat ttctgcactt
     2341 atcttaggat tttccttaat atacattctt gcggcatcct taccttgacc aattttctca
     2401 ccattaaatt caagccaagc tccgctcttt tttacaacac ctaaatccac accaacatct
     2461 attaaattac cttctttgct aattccttcg ttaaataaaa tatcaaattc tgcagatcta
     2521 aatgggggag cgactttgtt ttttactacc tttgcttttg ctctaatacc gtaaaactcc
     2581 tcacctcttt ttaatgtgcc tatttttcta atatcgattc gaattgaagc ataatatttg
     2641 agagctttgc ctccggcggt agtttcagga tttccgaaca tcacaccaat cattgatctt
     2701 agctggttag tgaaaaccac agtacatttt gatctgctta ttgccccgct taatcttctt
     2761 aaagcttgac tcattaacct tgcttgcatt gccacgtgac tatctccgat atcaccttct
     2821 atttcagccc gaggcacaag tgccgcaacc gagtcaatag ccaccaaatc cacagcattt
     2881 gatcttatta aagtttctgc aatatccaag gcttgttcac ctgtatcagg ttgtgagact
     2941 aaaagatttt caacatcaac tccagttttt tctgcatagg aaggatcaaa agcattttca
     3001 gcatcaatta aagcagcagt accgcccagc ttttgggttt ccgcgataat gtgttgaacc
     3061 aatgtagttt tacctgaaga ttcaggtccg aaaatctcaa taatcctacc tctgggaatt
     3121 ccacctacac ccaaagccat gtccaaagca atggaacctg tgggtatcac cgggatagtt
     3181 ctttcgccca ccgaaccacc taacttcata attgaaccct ttccgaaatt tttttctatc
     3241 tgatcaatag ctgtttttaa agccagatct tttcctgcct gagctggaga tttttcttta
     3301 atttctttaa gtttagaaga tgtttttgct gccatatgct gcctccttgt ttttaaataa
     3361 taaatttatt ttaccagtac tggtatattt atcaattaga attttctatt tttaatagag
     3421 aagttgtttt gagtatgtga tatattacga cgtctatatt taactaaaat ttcttaagtc
     3481 tttttaatgt ctcctctttt cctagtaaac caaggacatc aaatagagga ggggtagatt
     3541 cacaccctgt cgtgatatat cttattgtca taaaggcctc tttaggctta tatgaaaatt
     3601 ctgcaagttt ttcgtgagag atggcatcaa ggtcaggaac gttccatttt tcgctcggga
     3661 tatttttata aacttcaatg aaattatcaa ttatttcgtg agtttttttt gagtcacctg
     3721 aaaatttttc cgcagttttt ttatctattt tgggttcttc aaagaaataa aaagtaatgt
     3781 cgttaaactc actaagtttc ttcattctct ctcgaacaat ttccaatatt tttaaatttc
     3841 tagcatcagt agaatttttc aaattaagtt tttctgaata ttcctcgtgc cactttaata
     3901 ctctattcca taaatcttct aaaggtaaat ttctgatata aaggccgtta aaccacagaa
     3961 gtttctgtct atcaaaagcg gccatttctg atttttgaat tttttctatt gagaagtctt
     4021 taacaaaatc ttccagtgaa taaacttccc gctcggttcc ggggttccat cctaaaaaca
     4081 tcatgaaatt taaaagggct tccggaagat agccttcttg taaaaatcca agggcactca
     4141 ccgatcccgt tctttttgac aattttttgg tgctgtcggt ttccctcaat aaaggaaggt
     4201 gaataaattt cggcttctcc caaccaaaga attcatataa tagtacgtgt tttggggtag
     4261 aaggaagcca ttccctgccc cttagaacgt gggttatatt cataagatga tcgtccacca
     4321 ctacagctaa atgatatgtt ggaaaaccat ctgattttat taaaacttga tcatcc
//
LOCUS       DAOTFA010000040         4287 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate AMR_MDS_3115
            NODE_15101_length_4287_cov_7.460302, whole genome shotgun sequence.
ACCESSION   DAOTFA010000040 DAOTFA010000000
VERSION     DAOTFA010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868139
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (wastewater metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:36:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 536
            CDSs (total)                      :: 521
            Genes (coding)                    :: 520
            CDSs (with protein)               :: 520
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4287
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15101_length_4287_cov_7.460302"
                     /isolate="AMR_MDS_3115"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..447
                     /locus_tag="P5014_01880"
     CDS             <1..447
                     /locus_tag="P5014_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56892.1"
                     /translation="PTVIYTDKQIEKTRKVITSEQELAEFLNLIDETGILTVKEKVDF
                     EKNVVVAVSTETNEETGHKIKIRKVYEDKVKDKILIEFEEVNPEDDCKMEIDKNIAVD
                     LVMLSKTDAKVDFEKVTKVIECEDSESDNENTDESTESNSKTESTE"
     gene            466..1458
                     /locus_tag="P5014_01885"
     CDS             466..1458
                     /locus_tag="P5014_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013688049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HRY56893.1"
                     /translation="MLNLRLKLNQDVFLGEEKKSPRDGFGDALSELADKNKSIVAMSA
                     DLSESVKLKTFSQKYPERFIQTGIAEQNMCSIASGLAMEGKIPFVVTHAIFLTSRAWD
                     QIRLSICMANANVKICGSHEGFSNGPDGASAEPLEDIAIMRVIPNMTVINPIDYEQTK
                     KAVLEISKHTGPVYLRFSKAELPNITTKETPFKIGKADTLVEGDSVTVISCGGITYQA
                     LEAVKNLKAKYKINAELIASPTIKPLDEYKILESVKKTGRCVTVEEHQVIGGLGSAVS
                     ELLSEKLPSPLLRIGVGDTFGESGDYKDLLNKYGLSSHHIEEKVINFLKDKGKK"
     gene            1459..2406
                     /locus_tag="P5014_01890"
     CDS             1459..2406
                     /locus_tag="P5014_01890"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HRY56894.1"
                     /translation="MDILTIGDSCIDVTMHVSNADEVNMSGSDKQQICFLHGSKIPVD
                     RFYSAVGGNNVNVATGCNRMGLKTAVYTEIGDDANGNLIVDELNKLGINTEFCIKNKG
                     EVTNVNSIVVHNYERTIFSYHAPRNYKVRDWEKPTFLYYTSIGEGFEKFQMELIDYIE
                     KNRDIGIIFNPGTQQMKAGYNSIKEFLEVTNILILNLDEAVRLVGEKPLPKMHIDLQK
                     LGPKLTVITDGKNGASGFDGENLVKVSAYTDGREVKDKTGAGDAFSSGFISAIFHKKP
                     LKEALGWGSINSFGEITKESAGTGIFNKKEMEKITNEIM"
     gene            2443..3069
                     /locus_tag="P5014_01895"
     CDS             2443..3069
                     /locus_tag="P5014_01895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56895.1"
                     /translation="MKIGAFFERNKPIFIIGIVMFVIFSIIIVSSILMPKKETEYTEI
                     DEADKEFYKVAYEDLGKNEFEGTDEESEKKEEPAKQEVNMDEIFGVLQVDYTEEGFVP
                     RVTRIRVGQNVNWTNKTDKIIYLKQIKPYYKEFEESLSIKPNESFKFKMTEIGIWTYE
                     ETESGDWGSIEAKPLPSVMPEPKPKTETPATEDLKSTTEPTAITTPQL"
     gene            complement(3179..3811)
                     /gene="nth"
                     /locus_tag="P5014_01900"
     CDS             complement(3179..3811)
                     /gene="nth"
                     /locus_tag="P5014_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012991299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HRY56896.1"
                     /translation="MDKFEYAKEVARKLQKSHPEPKTELVHSDEYQLAVAVMLSAQTT
                     DKKVNQVTPELFKKYPDWESLANADIGEVGKLINQVNFYKGKAERLVKAGQVVVAEFG
                     GKLPKNIEELTKIPGIARKSANVITQELWGIAEGIVVDTHVSRVSNRLGLTKETDPKK
                     IERDLMKIIPKNYWRNFSGSAVLHGRYVCIARKPKCQDCVLNDICPSAFK"
     gene            3881..>4287
                     /locus_tag="P5014_01905"
     CDS             3881..>4287
                     /locus_tag="P5014_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56897.1"
                     /translation="MEEEIVKKTIVIWHDCNPCITKSLINYLNWTYLTKHSGTMLNIV
                     YLDELKERNLEESSLIKILRDCEALVYSCDSFHDIKASEEEWEVIKSQKWYAVSWNID
                     KKNNKKDEGCQLGLSIFTIKNVIFIEKEGLIKL"
BASE COUNT         1573 a          677 c          851 g         1186 t
ORIGIN      
        1 cctaccgtaa tttacaccga taaacaaata gaaaaaacaa gaaaagtaat aaccagcgaa
       61 caggagttag ctgagtttct aaatcttatc gatgagacag gtatattgac ggtaaaagaa
      121 aaggttgatt ttgaaaaaaa tgttgtcgtt gcggtgtcaa ctgaaacgaa tgaggaaact
      181 ggacataaga ttaagataag aaaggtttat gaggataaag taaaagataa gatattaatt
      241 gaatttgagg aagttaaccc tgaagatgat tgtaagatgg aaatagataa gaatattgca
      301 gtggatttag taatgctttc taaaacagat gcaaaagtag attttgagaa agtcacaaaa
      361 gttatagagt gtgaagacag tgaaagtgat aacgagaata cggatgaaag cacagaatca
      421 aactccaaga cggaatctac agaataaaat aaatatgact gccctatgct taatttaaga
      481 ttaaaactaa atcaagatgt ttttttaggc gaagagaaaa aatctcccag agatggtttt
      541 ggtgatgcgt tgagtgaatt agcagataaa aataaatcga ttgtggctat gtcagctgat
      601 ctttcagaat cagtaaagtt aaagaccttt tctcaaaaat atccggagag atttatacaa
      661 acaggaattg ccgagcagaa tatgtgctct atagcctcgg gacttgctat ggaaggaaaa
      721 ataccatttg ttgtcactca cgctatattc ctgacatcga gagcttggga ccaaataaga
      781 ctctctattt gtatggcaaa cgcaaatgta aaaatatgcg gatctcacga aggattttca
      841 aatggacctg acggagcctc cgcagaaccc ctggaagaca ttgcaataat gagagttatc
      901 ccgaatatga cagttataaa tccaatagat tacgagcaaa caaaaaaggc agttctggaa
      961 atatcaaaac acacggggcc tgtttatcta agattctcta aagccgaact ccccaatatc
     1021 actaccaagg aaaccccttt taaaatagga aaagcggaca cgttagtaga aggagacagt
     1081 gttacagtta tttcctgcgg aggaattact tatcaagcac tcgaagcagt aaaaaacctt
     1141 aaagcaaaat ataagataaa cgcagagcta atagcctctc ctactataaa accactggat
     1201 gagtacaaga tattggaatc tgtaaaaaag acggggagat gtgtaaccgt tgaagaacat
     1261 caagtaatag gcggcttagg tagcgctgtt tccgaacttt tgtctgaaaa gctaccctcc
     1321 ccgcttttaa gaattggggt tggtgacaca tttggagagt cgggagatta caaagattta
     1381 ttaaacaaat atggattgag ttcccatcat atagaagaaa aggtaataaa ttttttaaaa
     1441 gataaaggta aaaaataaat ggatatttta acaattggag actcgtgtat agacgtaaca
     1501 atgcatgttt caaatgccga tgaggtaaac atgtcaggat ctgacaaaca gcagatatgc
     1561 tttttacatg gcagtaagat acccgtagac agattttact ctgccgtagg aggtaacaat
     1621 gtaaatgtag ccacaggatg caacagaatg ggtttaaaaa ccgcagttta tactgaaatc
     1681 ggggacgatg ctaatggtaa cctgatagta gatgaactca ataaactggg tatcaataca
     1741 gaattttgta ttaaaaataa aggggaagta acaaacgtaa attcaatagt tgtacacaat
     1801 tacgaaagaa caattttttc atatcatgca cccagaaatt acaaagtaag agattgggaa
     1861 aaaccaacat tcctctacta cacatcaatc ggtgagggtt ttgagaaatt ccaaatggaa
     1921 cttatagatt acattgaaaa aaacagagat attgggataa tttttaatcc tggaacacaa
     1981 caaatgaaag cgggatataa ctcaatcaag gaatttttag aggttactaa tattttgatt
     2041 ttaaatctgg atgaggctgt aaggctcgtt ggggagaaac cattgcccaa gatgcatata
     2101 gatttacaaa aactcggccc taagttaaca gtaatcacag acggtaaaaa cggagccagc
     2161 ggttttgacg gtgaaaatct tgttaaagtt agtgcttaca cagacggccg ggaagtaaaa
     2221 gacaaaacag gggctggtga tgctttttct tcgggattta tttcagcaat ttttcataaa
     2281 aaaccattga aggaagcttt aggctgggga tcgataaact catttgggga aataacaaag
     2341 gaaagcgcag gaacgggaat attcaacaaa aaagaaatgg aaaaaatcac gaacgaaata
     2401 atgtaatcta ctagtgtatt aataaaattg tataatctta atatgaaaat aggagcgttt
     2461 tttgaaagaa ataagccaat attcataatc ggaatcgtaa tgtttgttat cttctctatc
     2521 ataattgtgt cttctatcct tatgcctaaa aaagaaaccg aatacaccga aatagacgag
     2581 gctgataaag aattttataa agtggcctat gaagatttgg gtaaaaatga atttgaagga
     2641 acagatgaag agagcgaaaa aaaggaggag cctgctaaac aggaggtcaa tatggatgag
     2701 atattcggtg tgttacaagt tgactacact gaagaaggtt ttgttccaag agttacaaga
     2761 atcagggttg gtcaaaatgt aaattggact aataaaactg ataaaatcat atatttaaag
     2821 caaattaaac cctattacaa agagttcgag gaatcactaa gcataaaacc aaatgaaagc
     2881 tttaaattta aaatgacgga gataggtatt tggacttacg aagagactga gtccggagat
     2941 tgggggtcta tagaggctaa acctttacca agtgttatgc cagaaccaaa acctaaaacg
     3001 gaaactccgg ctacagaaga tttaaaatca acaacagaac ccacagctat taccactccc
     3061 caactttagt atctatccta aaaatcgtta taacacctaa taaatttttt acaaaatttg
     3121 aataaaagat acaaggcccc ttcctatgta agtaggagat tataagtagg aatgaaagct
     3181 atttaaaagc actcggacaa atatcattta aaacacagtc ctgacatttt ggttttcttg
     3241 ctatacaaac ataacgaccg tgcaatactg ctgaacctga aaagtttctc caataatttt
     3301 tgggtatgat tttcatcaaa tctctttcga tttttttagg atcggtttct tttgtaagac
     3361 cgaggcgatt tgacactctc gatacgtgcg tatctaccac aattccttcg gctattcccc
     3421 acaactcttg agtgataacg ttggcacttt tccttgcaat accgggtatc ttcgtaagct
     3481 cctcaatatt ttttgggagt ttccctccaa attcagcgac aacaacctga cctgctttta
     3541 ctaacctctc tgcttttcct ttataaaaat tcacctgatt aattagcttg cctacctcac
     3601 ctatatctgc atttgccagt gattcccagt ccgggtattt tttaaaaagt tcgggcgtaa
     3661 cttggtttac ttttttatca gtagtttgag cggaaagcat cacagcaact gcaagttggt
     3721 attcatcaga atgaactaac tcggtttttg gctctgggtg ggatttttgt agttttcttg
     3781 ccacctcttt tgcgtattca aatttatcca tgcggcaatt atacattgaa tttttttctt
     3841 atatgtggaa tgataatcaa aaataaaaca ggaggaagag atggaagaag aaatagtgaa
     3901 aaaaacgata gtgatttggc atgactgtaa cccctgtatc accaagagtt taataaatta
     3961 cctaaattgg acatacttaa caaaacattc cggaactatg ttaaacattg tttacttaga
     4021 tgagttaaaa gagaggaatc tcgaagaatc aagtttaatt aaaatcttaa gagattgtga
     4081 agctttagta tattcctgcg attcttttca cgatataaag gcgtctgagg aggaatggga
     4141 agtaatcaaa agccaaaagt ggtatgcagt atcttggaac atagataaaa agaataataa
     4201 aaaagatgag gggtgtcagt taggtctttc tatttttacc attaaaaatg taatcttcat
     4261 cgaaaaagag ggattgatta aattaat
//
LOCUS       DAOTFA010000041         4210 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate AMR_MDS_3115
            NODE_15480_length_4210_cov_6.791095, whole genome shotgun sequence.
ACCESSION   DAOTFA010000041 DAOTFA010000000
VERSION     DAOTFA010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868139
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (wastewater metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4210)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4210)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:36:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 536
            CDSs (total)                      :: 521
            Genes (coding)                    :: 520
            CDSs (with protein)               :: 520
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4210
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15480_length_4210_cov_6.791095"
                     /isolate="AMR_MDS_3115"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..854)
                     /locus_tag="P5014_01910"
     CDS             complement(<1..854)
                     /locus_tag="P5014_01910"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRY56898.1"
                     /translation="MKIVMTGGHHSSALPVIKKLKEKIPGVEVLWMGHKHSMQRDKNP
                     TLEYIEITSLGIPFYDLKAGKVYKTYNIGRLLKVPLGFFQAFWFLLKNRPDAIMSFGG
                     YLAVPVVIAGYILGIPSLTHEQTVVTGYSNKLISRFTKKVLVSWKESEKYFPTDKVLY
                     VGIPLRKEIFHISSDSFNFDNDLLTIYISAGKTGSHIINENVYHSLEKLLTFCNIIHQ
                     SGDHSLYKDFEKLESKYTEIRNTVRGKYYLRKFVLEDEIGEAYMKADLVVSRSGAHTM
                     SEIIALQKP"
     gene            complement(882..2060)
                     /locus_tag="P5014_01915"
     CDS             complement(882..2060)
                     /locus_tag="P5014_01915"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HRY56899.1"
                     /translation="MKKRNSRKTDISGRKFFIAVTSFLLFGLLMIADSTSVSSKNLYG
                     DPYRFVILQATWAVAGLIAFYFFYRIDYRSLVKISRSLFLFNLLALIVLAIVGLMPCS
                     TSIPFSPCVNGANRWLYLNPAPLPAIPFLGVLGFQPGELAKLSVIVYLASQLSLISQK
                     DKSAKNKKPFEVYLKITVLMAGLIILQPNMSTAAMVFILGTIIYFVSGAPLKPIFITI
                     PIFLLLAILVILSSPYRMARLKAMISGEDSSESSYHVKQALVALGSGGFSGVGFGQSK
                     QKYQYLPEVASDSIFAIIGEEFGFIGTVVVVLAYGLFIFKGFRIAKKSQDMFGKLVSI
                     GISSWLGIQFFINVASMTKLIPMTGVPIPLISYGGSSLVFSLIGLGILANVQKNSSLD
                     "
     gene            complement(2076..3176)
                     /locus_tag="P5014_01920"
     CDS             complement(2076..3176)
                     /locus_tag="P5014_01920"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HRY56900.1"
                     /translation="MGTVGLMLDLLLLFLACLIAIVLYPLWIDFVYKFQMGESIRGDG
                     PESHKRKEGTPTMGGMVFVFTVAVVSLVFNRSRTQTLFPLFIATLAGLLGMLEDFTKV
                     YKKSGLPGFFHYHLGRFFRKRSEGFLKFSIFGKIWSIFKEASRLVGSSDDSGIQTYKK
                     FIIQGLIAGFISYWTYFKLGWDYIWFPLLGNVHIGFGYPVVIFFLFIGILNAVAFTDG
                     LDGLAGSLALISFISFWILSKILGYNSLAGFCATFVGAMIPFLYFNIFPARIFMGNVG
                     SYVLGAVMAMLAVVMHREVAFIFIGMMFFVDGISSPLQQIVYKITHKRLFRMAPIHHH
                     FEVLGWPETKITLRFVLFGVFFAFVGIFVAIL"
     gene            complement(3158..>4210)
                     /locus_tag="P5014_01925"
     CDS             complement(3158..>4210)
                     /locus_tag="P5014_01925"
                     /inference="COORDINATES: protein motif:HMM:NF012497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56901.1"
                     /translation="STLLAGPLLYRFGKAFIPKYKSSDESLRPINRFIDTWNFLGFQV
                     QDDEEFIKIFNDGSAKGCSINFKVTSHTATDNAIISSVFVPGETVISRASDEPEVEDL
                     INFLNGIGGSVEKLESGKIKITGTNIFKETSFDVCSDKSEIATFAALAVLTKGNISIK
                     NVKRDLILQFINFLNKIGARFEFSDDELRVWRHSEELSPQNLEISPTPGFLPEWQSLA
                     VLLLTQAVGESIIHDTVYVDRYGYCVDLNRMGAKIDIVTPSEVGLATVVSDDSYDIEK
                     EGEPKTVAKVSGPTKLKGERLTLENFKYGAVLILAALCAEGKSEIIGLDIVDCFFEGF
                     VDKLISLGAKIWEQSD"
BASE COUNT         1491 a          859 c          619 g         1241 t
ORIGIN      
        1 ggtttttgta atgctataat ctccgacata gtgtgagcac ctgatcttga aactactaaa
       61 tccgctttca tgtaagcttc ccctatctca tcctccaaaa caaacttcct taaatagtac
      121 tttcctctaa ctgtatttct aatctccgtg tacttgctct ctaatttttc aaaatccttg
      181 tacaaagagt ggtctcccga ctgatgaatg atattacaaa acgtaaggag cttttctaaa
      241 gaatgataaa cattttcatt aattatatga gaaccggttt ttcctgcaga tatgtagatt
      301 gtaagtaaat cgttatcaaa attaaaagaa tctgatgaga tatggaatat ttccttcctt
      361 aggggaattc ctacatataa aaccttgtct gtaggaaaat atttttcact ttctttccat
      421 gatactaata cttttttagt aaatctggaa ataagtttat tagaatatcc tgtgaccaca
      481 gtctgctcat gggtaagaga gggtatacca agtatataac ctgcaattac caccggaacg
      541 gctaaatatc ctccaaaaga cattatcgca tcaggtctgt tttttaataa aaaccaaaaa
      601 gcttgaaaaa atcccagagg aaccttaagc aacctaccaa tattataagt tttatatact
      661 tttcctgctt tcaaatcata aaaagggata cctaaggaag taatttcaat gtattccaga
      721 gttgggttct tatctctctg cattgaatgt ttatgaccca tccataatac ttcgacgccg
      781 ggaatttttt ctttaagttt tttgattacc ggtaatgcgg agctgtggtg tccgccagtc
      841 attacaattt tcatatgatt attctattct aaataagaaa tttaatccaa cgatgagttc
      901 ttttgaacat tggctaaaat acctaagcct attaacgaaa aaaccaacga ggacccaccg
      961 taggatatga gtggaatcgg cacaccggtc ataggtatta actttgtcat tgaagcgaca
     1021 ttaataaaaa attgaattcc cagccaagaa ctaatcccga tggaaactaa ttttccaaac
     1081 atatcttgag actttttagc aattctaaaa cctttaaaaa taaaaagacc ataggctaaa
     1141 actacaacca ccgttcctat aaatccaaac tcttctccga tgattgcaaa aatagaatcg
     1201 gaagccactt caggaagata ttgatacttt tgtttggatt gtccaaaacc aacaccagaa
     1261 aagcctccgg aacctaaagc tactaaagct tgtttaacat gataactaga ctcactgctg
     1321 tcttctcccg atatcattgc ctttagtctg gccatcctgt aaggtgacga caatataacc
     1381 aaaattgcta aaagtaaaaa aatagggata gtgataaaaa ttggttttaa aggagcccct
     1441 gaaacaaaat aaattatcgt tcctaagata aaaaccatcg cggcagtgga catatttggt
     1501 tgaagtatta ttaatcctgc cattaacaca gtgattttta agtacacttc gaatggtttt
     1561 ttattttttg cagatttgtc tttttgagat atcaaactta attgagaagc taaataaact
     1621 atcactgata attttgccaa ctcaccgggt tgaaatccca atactcccag gaaaggaata
     1681 gccggaagag gagcgggatt taaatataac catctgtttg caccattaac gcagggagaa
     1741 aaaggtatag acgtggaaca cggcattaac ccaactatag ctagaactat aagagcaagt
     1801 aaattaaata aaaataatga tctagatatc ttaaccagac ttctataatc gattcgatag
     1861 aaaaagtaaa aagctatcaa cccggctacg gcccaagtag cttgtaaaat aacaaatcta
     1921 taaggatcgc cataaagatt tttggatgaa acagaggtag aatcggcaat catcaacaaa
     1981 ccaaataata aaaaggatgt aacagcaata aaaaattttc ttccggaaat atctgttttt
     2041 cttgaatttc tttttttcat ttaatgcgcg atattttata gaattgccac gaatatccca
     2101 acaaatgcaa aaaacacacc aaaaagaaca aatcttaagg taatctttgt ctcaggccag
     2161 cccaaaactt caaaatgatg gtgtatagga gccattctaa ataatctctt atgtgtaatt
     2221 ttatatacaa tttgctggag agggctggat atcccgtcaa caaaaaacat cataccaata
     2281 aatatgaagg ctacttccct atgcataaca acggcaagca tagccataac agctcccaga
     2341 acatatgaac ccacgttacc cataaatatc cttgcgggaa agatattaaa atataagaaa
     2401 ggaatcatag cacctacaaa agtggcacaa aaaccagcta aagaattata tccaagaatt
     2461 ttagataata tccaaaatga aatgaatgaa attaaagcaa ggcttcctgc taaaccatca
     2521 agtccatctg taaaagcaac tgcgtttaaa atccctatga ataagaagaa tattactact
     2581 ggataaccaa atcctatatg aacattaccc aaaagtggaa accaaatata atcccatcct
     2641 aatttaaaat atgtccaata agaaataaat cctgctatta aaccctgaat aataaatttt
     2701 ttataggttt gtattccact gtcatccgaa cttcctacca atctcgaagc ctctttaaat
     2761 attgaccaaa tttttccaaa aatactaaat tttaaaaaac cttcagatct ttttctaaaa
     2821 aacctgccta agtgataatg aaaaaaacca ggtagaccag actttttata tactttagta
     2881 aaatcttcca acatacccaa caaaccagcc aatgtagcaa taaaaagagg gaagagagtc
     2941 tgtgttctgg atcggttgaa gactaaacta actacggcta cggtaaaaac aaaaaccata
     3001 ccacccatcg tcggggtgcc ttcttttctt ttgtgacttt ccggaccgtc gcctctgata
     3061 ctttcaccca tctggaactt atatacaaaa tctatccaaa gcgggtacaa gacaatagca
     3121 attaaacaag ccaaaaacaa taacaataaa tcaagcatta atccgactgt tcccatattt
     3181 ttgctcctaa tgaaataagt ttatcaacaa atccctcaaa aaaacaatca actatatcca
     3241 atccaattat ctcactttta ccttcagcgc aaagtgctgc taaaattaaa acagcaccgt
     3301 atttaaaatt ttccaaggtt agcctctctc cctttaattt tgttggccct gatacctttg
     3361 ctactgtttt aggctctcct tctttttcaa tgtcgtagga gtcatcacta accacagtgg
     3421 ccaaaccaac ctccgaagga gtaactatgt cgatttttgc acccattcta ttcaagtcaa
     3481 cgcaataccc atacctatca acatacacgg tatcatgaat aatactttcc cccacagctt
     3541 gtgttaacaa caaaactgca agtgattgcc actccggtaa aaaaccaggt gtcggagata
     3601 tttctaaatt ttgcggactt aattcctcac tgtgtctcca aactcttagc tcgtcatctg
     3661 aaaactcaaa tctcgcccct atcttattta aaaagttaat aaactgcaat attaaatctc
     3721 gtttaacgtt tttaattgag atgttacctt tagttaaaac tgccaatgcg gcaaacgtgg
     3781 ctatttcaga tttatctgaa caaacatcaa aggaggtctc tttaaaaata ttcgtaccgg
     3841 taattttaat tttcccagat tcaagcttct ctacagagcc tcctatccca tttaaaaaat
     3901 taatcaaatc ctctacttca ggttcatcag atgctctgga tataacggtc tcccccggaa
     3961 caaataccga agagatgata gcgttgtcag tcgcagtgtg gctggtaacc ttaaaattta
     4021 tactacatcc ttttgcagaa ccatcattaa atatttttat gaattcctca tcatcctgaa
     4081 cttgaaaacc taaaaaattc caggtatcta taaatctgtt aatcggccta agactctcat
     4141 ctgatgattt atattttgga ataaaagcct tcccgaacct atataaaaga ggcccagcta
     4201 ataaagtgga
//
LOCUS       DAOTFA010000042         4022 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate AMR_MDS_3115
            NODE_16498_length_4022_cov_6.628939, whole genome shotgun sequence.
ACCESSION   DAOTFA010000042 DAOTFA010000000
VERSION     DAOTFA010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868139
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (wastewater metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 4022)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4022)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:36:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 536
            CDSs (total)                      :: 521
            Genes (coding)                    :: 520
            CDSs (with protein)               :: 520
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4022
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16498_length_4022_cov_6.628939"
                     /isolate="AMR_MDS_3115"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..704
                     /locus_tag="P5014_01930"
     CDS             <1..704
                     /locus_tag="P5014_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019189969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HRY56902.1"
                     /translation="KDIQETSRGTEIVVSRADPDFVVRLFEQEVPEISSGVVVIEAIA
                     REAGSRTKMAVSSKDEKVDPVGSCVGQKGVRVQSIISELFGEKIDIVPYSDNLEKFIA
                     ASLSPAKVTEVELERKEKRAIVTVPEDQQSLAIGKEGQNARLANKLTRWKIDIKGVAG
                     IFSSEQAVEGSEKVEKDDKVIGVWDAEIKKYNEAEEIKKKEELVEENDSSEQPEASED
                     NQEPEKAEETQPDQE"
     gene            792..2282
                     /locus_tag="P5014_01935"
     CDS             792..2282
                     /locus_tag="P5014_01935"
                     /inference="COORDINATES: protein motif:HMM:NF012239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRY56903.1"
                     /translation="MSEDIKNKTEDYIRAPIVTIMGHVDHGKTSILDRIRKTNVQEKE
                     YGGITQHIGAYQISHNGKKITFIDTPGHAAFTQMRARGGRAADIVVLVVAAEEGVKPQ
                     TKEAISHIRAAKVPFIVAINKIDKPGADPRKVKQELAQENVLVEDWGGSEICVEMSAK
                     TGEGMDTLLDAILLLSEMISIPADPLGEMEAIIIESRLDRKKGVVVSCILKNGTLRVG
                     DTVSASGINAKIKSMTGYDGKIMKEVGPSDPVELLGFSVVPHVGDILLMKGSELAELS
                     RDDNKIEIIGKDAKRMVSMVLKADTEGTLEAVKASLSNLVTSSAETTYALKLLHTGTG
                     DITESDVMLAQSVKGVVVGFDIRISPAVEDLAESLGVFIKSYRTIYDLIDDAAKFLEG
                     TAVNEMSKVKGRAKVIKIFKLDSGDIIAGCKVLAGALKVKSRVAIYNKNPADLTKEDE
                     PLFYGSIKKLKKGKDDIDVAGKDNECGVLLKPQFEEITADMYFEVL"
     gene            2490..3140
                     /locus_tag="P5014_01940"
     CDS             2490..3140
                     /locus_tag="P5014_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56904.1"
                     /translation="MNSEFDKTNQILSLIKEAGSIVILPSKTAGIDSFSSAVGLYYML
                     QAEGKEVMILYPGMVPQDFGDVEDINIADNSSARELVVSVDYSNTNASKVNYSTENDV
                     LHFSLSPIERDFDLSKIKSEIKGYDFDLIITVGAPSRDSLGRLLEDVGGTFGGVDILN
                     IDNSERNQRFGNINVVDPSFDSTSLLVLNHAIKWDLEISSKAAEALLKGISKRKGI"
     gene            3186..3455
                     /gene="rpsO"
                     /locus_tag="P5014_01945"
     CDS             3186..3455
                     /gene="rpsO"
                     /locus_tag="P5014_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_033016867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HRY56905.1"
                     /translation="MALSKEKKAQIIKTSRLHDADTGSPEVQVAILTESIAKLSDHLK
                     THKKDNHSRRGLLQMVNKRRRLISYLKGKDEDRYSQLVEKIELSK"
     gene            3580..>4022
                     /locus_tag="P5014_01950"
     CDS             3580..>4022
                     /locus_tag="P5014_01950"
                     /inference="COORDINATES: protein motif:HMM:NF013318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HRY56906.1"
                     /translation="MNKIVRKETNFAGKKLIFETGELALRADMSVKATYGDTVLLVTA
                     VSGDPSPEADFLGLRVQYEEKLYASGSIKSSRFVKRDGKATDDAVIARRSIDHAIRPL
                     FPKDYMDEVQIVVTVLSLDEEADPEFLSMLATSACLQASNIPFSGP"
BASE COUNT         1392 a          625 c          849 g         1156 t
ORIGIN      
        1 taaaagacat tcaagaaaca tcgagaggta ctgaaattgt tgtaagtaga gcagaccctg
       61 attttgttgt gagacttttt gaacaagaag ttccagaaat ttcttccggt gttgttgtta
      121 tcgaagcaat tgccagagaa gcaggttcca gaacaaagat ggctgtttct tcaaaagatg
      181 aaaaagtaga tccagttgga agctgtgtcg gtcaaaaggg agtaagagtt caatctatca
      241 tttccgagct ttttggtgaa aaaatagaca ttgttcctta ttccgataac ttagaaaaat
      301 tcatagccgc aagtctttct cctgcaaaag taacagaagt agagcttgag agaaaagaaa
      361 aaagagcaat tgttaccgtt cccgaagatc agcaaagttt ggctatagga aaagaaggtc
      421 aaaatgccag attagccaac aaacttacaa gatggaaaat agatataaaa ggtgttgcag
      481 gaatattttc aagtgaacag gctgttgaag gtagtgaaaa agttgaaaaa gacgataaag
      541 ttattggagt ttgggacgct gagattaaaa aatacaacga agcagaagaa ataaagaaaa
      601 aagaagaatt ggttgaagaa aacgattctt ccgaacaacc tgaagcttca gaagataacc
      661 aagagcctga aaaagccgaa gagacacaac ccgatcaaga ataaagtttg atttagaaag
      721 atgacgtttt taaaatcttt tgatcaaata atgattgaaa aaagtgtaat attaaattgc
      781 ttgtataaac tatgtctgaa gacattaaaa ataaaactga agattacatc agagctccca
      841 ttgtcaccat tatgggacat gtcgatcatg gtaaaacaag cattctcgat cgtataagaa
      901 aaactaatgt tcaggaaaaa gagtacggcg gtattacaca acatattggt gcttaccaaa
      961 tttctcataa cggtaaaaaa ataactttta tagatacacc cggccatgca gcatttactc
     1021 agatgagagc cagaggtgga agagctgccg acatagtcgt actagttgta gccgcagaag
     1081 aaggggtaaa acctcaaact aaagaagcta tttcacacat cagagcagca aaagttcctt
     1141 ttatcgtagc tataaataaa atagacaaac cgggtgctga tccgagaaaa gtaaaacaag
     1201 aattagctca ggaaaacgtt cttgtagaag attggggtgg aagcgaaatt tgtgttgaaa
     1261 tgtcggcaaa aaccggagaa gggatggaca ctcttcttga tgctatattg cttttatctg
     1321 aaatgatttc gataccggct gaccctttag gtgagatgga agccataatt attgaatccc
     1381 gacttgatag gaaaaaaggt gtggtggttt cttgtatttt aaaaaatgga actttaagag
     1441 ttggagatac agtttcagct tcgggaatta atgcaaaaat aaaatcaatg accggatatg
     1501 acggaaaaat aatgaaggaa gtcggtcctt cggaccctgt agagttgctc gggtttagcg
     1561 ttgttcctca tgtcggagat attttattaa tgaagggaag tgagttagct gaattatcca
     1621 gagacgacaa caaaatagag attattggaa aagacgctaa aagaatggtt tctatggtct
     1681 taaaagccga caccgagggt acgctggaag ctgttaaggc aagtttatca aacttggtta
     1741 cttcttcggc cgagacaacc tatgctttaa aactcctgca caccgggacc ggggatataa
     1801 ccgagtcaga tgtaatgctt gctcaaagtg ttaaaggtgt ggttgtgggt tttgatataa
     1861 gaatttctcc cgcggttgaa gatttggcag aatccttagg tgtttttatt aagtcttaca
     1921 gaactattta cgatctaatt gatgacgcag caaaattttt ggaaggaact gctgttaatg
     1981 aaatgtcaaa ggtaaaaggc agagccaagg tcataaaaat atttaaactt gattcgggcg
     2041 atatcatcgc cggatgtaaa gttcttgcag gagcactaaa agtaaaatca cgtgtagcta
     2101 tttataataa aaatcccgca gaccttacaa aagaagatga gccgttattt tacggttcca
     2161 ttaaaaagtt aaaaaaagga aaagatgata ttgatgttgc cggtaaagac aatgaatgtg
     2221 gtgtacttct taaaccccag tttgaagaaa ttactgcaga catgtacttt gaagttctat
     2281 agtctttaga tatttcttta taaatttata ccccaattta ccttgtcatt ataatgtttt
     2341 actgttttcc tgattttcta ggatcgccct caagcctcac tgacatttga cactataggg
     2401 ctaaagttgt acaattcttc aggttctggt atagtgttcc atcctctact tattatgtgt
     2461 tccctgctcg agtaaatttt aaataaaata tgaattctga atttgataaa acaaaccaaa
     2521 tattgagtct tattaaagaa gcgggaagta tagtaattct tccttctaaa actgcgggta
     2581 ttgattcatt ttcatccgcg gtaggtcttt actatatgct acaagccgaa ggtaaggagg
     2641 taatgatttt gtatcccggt atggttccgc aggatttcgg agatgtcgag gatataaaca
     2701 ttgctgataa ttcttctgca agagaacttg tggtctctgt tgactactcc aatactaatg
     2761 cttcaaaagt aaattactca accgaaaacg atgttttaca tttttcacta agtcctattg
     2821 aaagagattt cgatttatcc aaaataaaat ctgaaatcaa aggttacgat tttgatttaa
     2881 tcataactgt aggcgcccct tcgagagaca gcttgggcag attattggaa gatgtgggag
     2941 gaactttcgg tggggttgat attttaaata ttgataattc tgaaagaaat caaagatttg
     3001 ggaatataaa tgtagtagac ccgagctttg atagcacaag tttgctggta ttaaatcatg
     3061 ccattaaatg ggaccttgaa atttccagca aggctgcgga agctttactt aaaggaattt
     3121 ctaagagaaa gggtatttag tagttgattt attaagttag ttttggtata ttaccatagt
     3181 taattatggc tttatctaag gaaaaaaagg cacagataat aaaaaccagc agattacatg
     3241 atgctgatac aggatcacct gaagttcagg ttgctatcct tactgaatct attgcaaaat
     3301 tatccgacca tcttaaaacc cataaaaaag ataatcactc cagaagaggt cttcttcaaa
     3361 tggttaacaa aagaagaaga ttgatatcct atctaaaagg aaaagatgaa gataggtata
     3421 gccagttagt tgaaaaaatt gagctgtcta agtaaggaca gccggaaaaa ttaggatagc
     3481 gggtcttatt cattggatta ggaggcaggc tattatgcat cgagtgcata atattttgaa
     3541 tcttaatcct ctagataaca aacccctatg taagaaaaaa tgaataaaat tgtaagaaaa
     3601 gaaaccaatt ttgctggtaa gaaacttatt ttcgaaaccg gagaattagc tttaagagca
     3661 gatatgtctg ttaaagcaac ctatggagac acagttcttc ttgtaaccgc tgtttcagga
     3721 gatccttctc ccgaggctga ttttttaggt cttagagtcc agtatgaaga aaaactttat
     3781 gccagcggta gtataaaaag ttcgaggttt gttaaaagag acggaaaagc aactgacgac
     3841 gcagtgatag ccagaagatc tatagatcac gctattagac ctttgtttcc taaagattat
     3901 atggatgagg ttcaaattgt tgttactgtg ctttctcttg atgaagaagc cgaccccgag
     3961 tttttaagta tgttagctac ttcagcttgt ttacaggcaa gtaatattcc attttcaggc
     4021 cc
//
LOCUS       DAOTFA010000043         3913 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate AMR_MDS_3115
            NODE_17170_length_3913_cov_7.808709, whole genome shotgun sequence.
ACCESSION   DAOTFA010000043 DAOTFA010000000
VERSION     DAOTFA010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868139
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (wastewater metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3913)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3913)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:36:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 536
            CDSs (total)                      :: 521
            Genes (coding)                    :: 520
            CDSs (with protein)               :: 520
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3913
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17170_length_3913_cov_7.808709"
                     /isolate="AMR_MDS_3115"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..175)
                     /locus_tag="P5014_01955"
     CDS             complement(<1..175)
                     /locus_tag="P5014_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015908222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HRY56907.1"
                     /translation="MPLIKALILGIVQGLTEFLPVSSSAHLIIFPEILGWAEHSLSFD
                     TTLHLGTLFALIVV"
     gene            complement(179..844)
                     /locus_tag="P5014_01960"
     CDS             complement(179..844)
                     /locus_tag="P5014_01960"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRY56908.1"
                     /translation="MPKIIFETDSELNGRIRIVDVGSTRKMVVGNTIQSINPDSPACP
                     KLYWGQMSDSIKERFTDIQNILILGLGGGTLAHLLSRAYPDVNITSVEYDEVMIQLAK
                     EYFYVDKISNHKIIHEDALRIVVEPEENDITPSSLDLVVVDVLNGDKYPDLGKTGNFI
                     SAVKRLIRPGGHVVFSRIYTEFFQEEVNIFVDSLENYFSEVETEVVAGYTNSDNLLIF
                     GKV"
     gene            complement(888..2228)
                     /gene="serS"
                     /locus_tag="P5014_01965"
     CDS             complement(888..2228)
                     /gene="serS"
                     /locus_tag="P5014_01965"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR00414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HRY56909.1"
                     /translation="MLDINFIKNNSEVVKKVISEKNIKGVDIDNLLKIYDDYLDHLKK
                     SEDLRKSRNEISSSVSKVQGDERNTLIAKASIIKDELSAVELKLDQLKKDIEQILPSI
                     PNVVSEEMPVGKDEHDNLIIKVWDPVKGYIKFEEPLKYDDISYAPESAFSYKDHMELG
                     ESLDIIDIEQSGKVSGSRFCYLKKEAVLIQDAISNLLKMELQKRNFIPMIPPLLVKER
                     SLFGTSHFPEGRAQVYEIRNENVEDNNSLFLVGSAEPSNFSYFMDKVLKKEDLPIKVY
                     AQTTCFRSEVGSWGKDVRGIKRVHQFDKLEMNCVCTEDQDRAVFDEFIEINEWLFQQL
                     KIPYRVVNKCTGDCGYNASYFQYDVEAWRPKEKEYMEVGTDTMTTDYQARRLNIKYKD
                     KDGLKFARTVNDTGVAFGRAIIALLENHQKEDGSVEIPEALFPYLDFKIILPKV"
     gene            2424..2642
                     /gene="infA"
                     /locus_tag="P5014_01970"
     CDS             2424..2642
                     /gene="infA"
                     /locus_tag="P5014_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002262318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HRY56910.1"
                     /translation="MITKEVITKEGKVTETLMGGMFKVQFEDETTALAMISGKMRKQH
                     IRVLVGDKVKVEFSPHDLSRGRITFRLR"
     gene            2707..2823
                     /gene="rpmJ"
                     /locus_tag="P5014_01975"
     CDS             2707..2823
                     /gene="rpmJ"
                     /locus_tag="P5014_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004025773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HRY56911.1"
                     /translation="MKVRASIKPICKDCKIIKRKGVLRVICKRNPKHKQRQG"
     gene            2825..3211
                     /gene="rpsM"
                     /locus_tag="P5014_01980"
     CDS             2825..3211
                     /gene="rpsM"
                     /locus_tag="P5014_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011433188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HRY56912.1"
                     /translation="MPRIKGVDIPNEKRIEASLTYIEGIGLTTSKNILKMAKIDPDKR
                     ARDLTDGEIAEINNAISSLEIPIEGELRRIVTSNIRRLKEIKSYRGLRHHLGLPTRGQ
                     RTRTNARTRKGKKKTVGGQKKKLAKK"
     gene            3233..3607
                     /gene="rpsK"
                     /locus_tag="P5014_01985"
     CDS             3233..3607
                     /gene="rpsK"
                     /locus_tag="P5014_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014015761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HRY56913.1"
                     /translation="MAKKKKEKQISSTGKAYITAGMNNTIITITDDMGNTLFSGSSGK
                     SGFKGSRKSTPYAATKASEQAGAMAFNAGVREVAVFVKGPGMGRISSIKALKTAGLNI
                     VSISDQTPMPHNGCRPKNRRRI"
     gene            3609..>3913
                     /locus_tag="P5014_01990"
     CDS             3609..>3913
                     /locus_tag="P5014_01990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012341938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HRY56914.1"
                     /translation="MARYIGPKCRMCRREAVKLYLKGKRCESEKCPLNDKSQAPGQHG
                     TSRKALSVYGTQLREKQKAKRIYGLLERQFRNYINEALRARGVSGNILMQKLESRFD"
BASE COUNT         1407 a          706 c          634 g         1166 t
ORIGIN      
        1 aaacaacaat aagagcgaat aatgttccga ggtgaagcgt ggtatcaaag gacaacgagt
       61 gctccgccca tcctaagatt tccggaaaaa ttatcaaatg tgctgaagaa gaaacgggca
      121 agaattctgt caagccttga actattccta aaatgagagc tttaattaaa ggcatttttt
      181 aaacctttcc gaatattaat aaattatctg aatttgtata acctgctact acctctgttt
      241 ctacttcgct aaaataattc tccaagctat ctacaaaaat attaacttcc tcctgaaaaa
      301 attcagtata tatcctacta aacacaacgt gacctccagg tctgattaat ctttttacag
      361 cagaaataaa atttcctgtt tttccaagat cagggtactt gtcaccgttc aatacatcta
      421 ctactactaa atccaaggaa ctgggagtta tatcattttc ttcaggctct acaactattc
      481 ttaaggcgtc ttcgtgaatt attttatgat ttgagatctt atcaacataa aaatactcct
      541 tggctaactg aatcataacc tcatcgtact caacgctggt aatatttaca tcaggataag
      601 ctcttgataa aagatgtgct aaagttccac cacctaatcc taaaattaaa atattttgta
      661 tatcagtaaa acgttccttt atagaatctg acatctgccc ccaatataat ttaggacaag
      721 ctggagaatc aggattaatg gattgaatag tgtttccaac caccattttt ctagtactgc
      781 ctacatcaac aattctaata cgtccgttta attccgaatc cgtttcaaaa ataattttgg
      841 gcattttaag cccgtcaaaa acactcattt aataaatcgt aacataatta aaccttgggc
      901 agaataattt taaaatcaag ataaggaaat aacgcttccg gaatttcaac cgaaccgtct
      961 tccttttgat gattttccag taatgcaatg atggccctac caaaagcaac gccggtatcg
     1021 tttactgtcc tggcaaattt caaaccgtct ttatctttat atttaatatt caaccttctc
     1081 gcttgatagt cagtggtcat tgtatcggta cctacttcca tatattcttt ttctttaggt
     1141 ctccacgctt ctacgtcata ttgaaaatat gaagcgttgt aaccacaatc ccccgtgcat
     1201 ttgttgacaa ccctgtaagg aatctttaat tgttggaata accactcatt aatctctata
     1261 aattcatcaa aaactgccct atcctgatct tcggtacaaa cacaattcat ttctaattta
     1321 tcaaattggt gaactctttt tattcctctt acatccttac cccaagaacc aacctcactt
     1381 ctaaagcaag tggtttgagc gtacacttta atcggtaaat cttctttttt caaaacctta
     1441 tccataaaat atgaaaaatt cgacggctct gcagaaccca ccagaaataa agaattatta
     1501 tcttcaacat tttcatttct aatctcgtaa acttgcgccc tgccttcagg aaaatgagaa
     1561 gtgccaaaaa gggatctttc ctttacgaga agtggcggaa tcattggaat aaaatttctc
     1621 ttttgtaact ccatttttaa aagattggaa attgcatcct ggattaaaac ggcttctttt
     1681 tttaaataac aaaatctgct tcccgaaact tttccgcttt gctctatatc aataatatcc
     1741 aaactctccc ccagctccat atggtcttta taagaaaaag cggattcggg ggcatatgaa
     1801 atgtcatcgt actttaaagg ttcttcaaat tttatgtatc ccttaaccgg atcccaaact
     1861 ttaataatta aattatcgtg ctcatcctta ccaacaggca tttcttctga aacaacatta
     1921 ggaatactgg gtaaaatctg ctcgatatct ttttttaatt gatctaattt caattcgacc
     1981 gcagatagct catcttttat aatagaggct ttggcaatta aggtgttcct ttcatctcct
     2041 tgtactttag agacacttga agaaatctca ttcctggatt ttctaagatc ctctgatttt
     2101 ttcaaatgat ctaagtagtc atcataaatc ttgagtaggt tgtcgatatc tacaccttta
     2161 atattttttt cagaaattac ctttttgaca acctcactat tattttttat aaaatttatg
     2221 tccaacatct tttacattaa agcgcttttt cgttgctttt tcaataagtg gtggtaatat
     2281 aagtttctaa cattgagtta gaacaaatca gtggttaatt ctacaaacat ttgttacacc
     2341 gtaacaaagg tgtttttgta gaaaaaatac gcttattgac ctacaaaatt tgttctgata
     2401 aaatcaagcg cgtcatttaa aggatgatta ctaaagaagt aataacaaaa gaaggaaaag
     2461 taacagagac attaatgggc ggcatgttca aagtacaatt tgaagatgaa acaaccgctc
     2521 ttgcaatgat atctggtaaa atgagaaaac aacatataag agtcttagtc ggagacaaag
     2581 taaaagttga gttctctcct cacgatttat caagaggaag aatcactttt agacttagat
     2641 aagaccttgg ttttcttttt ttaatggtag caactgttgc ttccctttag ttataattaa
     2701 aaaaatatga aagttagagc atcgataaaa ccaatctgca aagattgtaa aataattaaa
     2761 aggaaaggtg tccttagggt catttgtaaa agaaatccta agcacaaaca aagacaagga
     2821 taatatgcca agaataaagg gtgtggatat accaaatgaa aaaagaatag aagcttctct
     2881 aacttacata gaaggtatag gcctaaccac atctaaaaat attttaaaaa tggccaagat
     2941 agatcctgat aaaagagcaa gagacttgac tgacggtgag atagcagaaa ttaataatgc
     3001 aatttccagt ttggaaattc ccatagaagg tgagttaaga agaattgtta catcaaatat
     3061 cagaagatta aaagaaatta aaagctacag aggacttaga catcacttag ggcttcctac
     3121 aagaggtcaa agaacaagaa ctaacgccag aacaagaaaa ggtaaaaaga agactgtagg
     3181 cggtcagaag aaaaaattag caaagaaata aaaggaaggt tattttaaat aaatggcaaa
     3241 gaaaaagaaa gaaaaacaaa tatcatcaac cggaaaagct tacataacag ccggcatgaa
     3301 caatacaata attacaatta ctgatgatat gggaaataca ttattttcag gaagttcagg
     3361 aaaatcaggt ttcaaaggtt ccagaaaatc aactccttat gctgcaacca aagcctcgga
     3421 gcaggcagga gcaatggcat ttaatgccgg cgttagagaa gttgcagttt ttgtaaaagg
     3481 acccggaatg ggaagaatat cttccattaa agctttaaaa acagcaggac taaacatagt
     3541 ttcaatttcg gatcagacac ctatgcctca taatgggtgc aggccaaaaa acaggaggag
     3601 aatataaaat ggctagatat ataggaccta aatgcagaat gtgcagaaga gaggctgtaa
     3661 aactttatct taaaggtaaa agatgcgaaa gtgaaaaatg tcctttaaac gataaatcac
     3721 aagcacctgg acaacacggg acaagcagaa aagctttatc agtttacgga acacaattaa
     3781 gagaaaaaca aaaagcaaaa aggatatacg gtcttttaga aagacagttt agaaattata
     3841 taaacgaagc tttgagagca agaggtgttt caggaaatat tttaatgcaa aaacttgaat
     3901 cgagatttga caa
//
LOCUS       DAOTFA010000044         3840 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate AMR_MDS_3115
            NODE_17631_length_3840_cov_6.109379, whole genome shotgun sequence.
ACCESSION   DAOTFA010000044 DAOTFA010000000
VERSION     DAOTFA010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868139
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (wastewater metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3840)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3840)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:36:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 536
            CDSs (total)                      :: 521
            Genes (coding)                    :: 520
            CDSs (with protein)               :: 520
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3840
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17631_length_3840_cov_6.109379"
                     /isolate="AMR_MDS_3115"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..799)
                     /locus_tag="P5014_01995"
     CDS             complement(<1..799)
                     /locus_tag="P5014_01995"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56915.1"
                     /translation="MSLGINISIPRKILLFLVEVFIKILWVFDKVTSLDLLYIGKTLL
                     RTIFLTIPLSFLVFLGYSISTGYLHKLYIAYFFPETNIIYKNFSNKPYPVLTGSEPLP
                     QTTALSLLAIDKKSNKTLVEKNINISLAPASTAKLMTAIISLDIYDLEEVLAVSDYCT
                     EVEGTKAFLPVNKIFKVKDLLNSMLIGSAGDSACVLATSKMSEQDFVEKMNEKVNSLG
                     MTSTHFSNPVGLDDAYGNNYSNTLDLYKLAVYATSISEIKKIVGTKDF"
     gene            complement(813..2105)
                     /gene="aspS"
                     /locus_tag="P5014_02000"
     CDS             complement(813..2105)
                     /gene="aspS"
                     /locus_tag="P5014_02000"
                     /EC_number="6.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014136784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA(Asn) ligase"
                     /protein_id="HRY56916.1"
                     /translation="MERTQIKNLKEKIGEKVKVSGFVQVRRDQGGIKFFVVRDVTGTV
                     QVVFLKGAGEVFNKAGEVSLESVVSIEGLVKEEKQAPGGFEISAESLEILSKAEPELP
                     IPVLEEKSGEPTDITKRLDWRWVDLRKEENLKIFKVWTELEKGFRSYYSQQGYIQIYT
                     PSLMGTASETGSDVFKLQYFEGEAYLAQSPQFYKQMAQASGFEKVFCVGPVFRAEPSF
                     TSRHMTEFTGWDFELSYIDSHLDVMSEEENLIVSGFTQLKNTVLPDLEIPSTPFPRIT
                     MEEAKEKLNKAGIKAGRKFDISGEEEIEICKIIKEETGHDFVFVTDWPAEARPFYHMR
                     YEDNPSLTKSFDLLYKGLEITTGSQREHRYDVLISQAKEKGMSVESLSDYLNFFKFGC
                     PPHGGAGIGPGRLVMKILNIPSVKEVTFLPRDVRRLKP"
     gene            complement(2108..3736)
                     /gene="recJ"
                     /locus_tag="P5014_02005"
     CDS             complement(2108..3736)
                     /gene="recJ"
                     /locus_tag="P5014_02005"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HRY56917.1"
                     /translation="MYTWKITGDYNQKKDISLSILEGRGVKDIDLFLKTPTLNESFES
                     LSSDFKKSLSKARDLIFSEMEKNTPILIFGDYDSDGINATAILYNFFKYEKKYDKVSY
                     FIPNRFEHNYGLSKLAIDEALDNYKSDEKVLFITVDVGVTAFEEISYIKKLGHSVILT
                     DHHQKPEKVPTPDLLVWNDGVCGAAIAWLLSKALGSKSRDGIVNASVATVTDLIPLTG
                     FNRIIVKKGLEVINSNPPLWIKKLFEVSSIKDIEITTYEIGWAVGPRLNASGRLKSSE
                     DSIRLLTEHDETVLEKLSSSLNSKNIERQEKTIEMYDMASEIDEKNLPKIIFSSNEKF
                     HEGVIGLVSAKLTQRYYRPSVVISLEDGYGKGSVRSIPGVNIIEILRKFNDLFVDLGG
                     HPMAGGFTIEKKNIPEMQKKVSQYIEKEFDDDIFVPVLNIDLKIPSDILNVKILDEID
                     KLKPFGMGNEQPLFLTENFVIVNSDIIGKDKTHLKLKLYDGNKYHKAVYFGGARYEKD
                     LAVGDKVDLVYMLNKNEYNGNTYLDLVIKDFRKV"
BASE COUNT         1208 a          765 c          584 g         1283 t
ORIGIN      
        1 aaaaatcttt tgtacccaca atttttttaa tctccgaaat agaagttgca tatacggcca
       61 acttgtacaa atccaatgta tttgaatagt tattcccata agcatcatcc aaacctaccg
      121 gatttgaaaa atgagttgaa gtcatcccca aggaatttac tttttcattc atcttttcta
      181 cgaaatcttg ctcactcatt tttgacgtcg ctaaaacaca agcggagtct cccgcggaac
      241 ctattagcat actatttaag agatctttca ctttaaaaat tttattaacc ggaagaaaag
      301 ccttggtacc ctctacttct gtgcaataat cggacactgc gagtacttcc tcaagatcat
      361 aaatatccaa cgagattatt gctgtcataa gtttagctgt agaagccggg gctaatgaaa
      421 tatttatgtt tttttcaacc agcgttttat tacttttttt atctatggct aacaaagata
      481 aagctgtagt ttgaggaaga ggctcggaac cggtaagtac agggtaaggc ttattgctga
      541 aatttttata gataatattc gtttcaggaa aaaagtacgc aatatataat ttgtggaggt
      601 atcctgttga tatcgaatat cctaaaaaaa caagaaaaga cagaggaatt gtcaaaaaaa
      661 tcgtacggag aagtgttttt ccaatgtata agagatcgag actagttact ttatcaaata
      721 cccaaagtat ctttataaaa acttcaacaa gaaacagcag aatttttctt ggtatggaaa
      781 tgttaatccc taaagacatt ttaaccgtag aattatggtt ttaatcttct aacatccctg
      841 ggaaggaaag tcacttcctt gacgcttgga atattaagaa ttttcatcac caatcttccg
      901 gggcctattc cggcacctcc gtgaggagga caaccaaatt taaagaaatt aaggtagtca
      961 ctcaaagatt caacgctcat tcccttttct ttagcttggg aaatcaaaac gtcataccta
     1021 tgttctcttt gtgagccagt ggtaatttca agacctttat atagaaggtc aaaacttttt
     1081 gttaatgatg ggttgtcctc gtatctcata tggtaaaaag gtcgtgcttc tgcgggccaa
     1141 tcggtaacaa aaacaaaatc gtgcccagtt tcttctttta ttattttaca aatttctatt
     1201 tcctcttctc cactgatatc aaactttctg ccggctttta tacccgcttt atttaatttt
     1261 tcctttgctt cttccattgt aattcttgga aaaggagttg aaggaatttc caaatcaggt
     1321 agtaccgtat ttttaagctg tgtaaaacct gaaactatta aattctcctc ctcggacatt
     1381 acatccaaat gagaatctat ataagaaagt tcaaaatccc aaccggtaaa ttcggtcata
     1441 tgacgggatg tgaaagaggg ctcagcccta aaaacaggac ctacacaaaa aaccttctca
     1501 aacccggaag cttgagccat ttgcttataa aattgagggg attgagccaa gtaagcttca
     1561 ccttcaaaat attgtaattt gaaaacatcg gaacctgttt cgcttgcagt acccatcaag
     1621 gaaggggtgt aaatttgaat gtaaccttgt tgactgtagt agcttctaaa acctttttcc
     1681 agctctgtcc aaactttaaa tatttttaaa ttctcctctt ttctcaaatc aacccatctc
     1741 caatcaagtc tttttgtaat atcagtaggt tccccgctct tttcttccaa aacaggaata
     1801 ggaagttcgg gctctgcttt agataaaatc tccaaactct cggcggaaat ttcaaaaccc
     1861 ccgggagcct gcttctcttc cttaaccaaa ccttcaatag aaaccactga ctctaaagaa
     1921 acctcacctg ccttattaaa aacctcgccg gctcccttta aaaaaactac ctgtaccgtg
     1981 ccggtaacat ctctaacaac aaaaaactta atcccgccct gatctcttct gacctgaaca
     2041 aagcctgaaa ccttgacttt ttcacctatt ttttctttta aatttttaat ttgtgttctt
     2101 tccattttta aacctttcta aaatccttaa tcactaaatc aagatacgta ttaccattgt
     2161 actcattttt attcaacata taaactaaat ctaccttatc tccaactgct aaatctttct
     2221 cgtatcttgc tccgccaaaa tatactgctt tgtgatattt atttccatcg taaagtttta
     2281 attttagatg cgttttgtcc ttacctatga tatcgctgtt aactattaca aaattttcag
     2341 ttaaaaatag tggctgttcg tttcccatac caaaaggctt taatttatca atctcatcaa
     2401 gaatttttac atttaaaata tcggagggaa tttttaaatc aatatttaat acgggtacaa
     2461 aaatatcatc atcaaattct ttttcaatgt attgggatac ttttttctgc atctcgggaa
     2521 tatttttctt ttcaattgta aaaccacccg ccatggggtg gcctcctaaa tcgacaaata
     2581 aatcattaaa ttttcttaag atttcaataa tatttacgcc gggtatagac ctgacagaac
     2641 cttttccgta accatcttct aatgagatca ccacggaagg tcggtaatat ctttgagtta
     2701 gcttcgcaga tactaaacca ataacgcctt catgaaactt ttcattggat gaaaaaatta
     2761 ttttaggaag gtttttttca tctatttcag aggccatgtc gtacatctca attgtttttt
     2821 cctgcctctc aatatttttc gaatttaaac ttgaagatag cttctctaaa accgtttcgt
     2881 catgttcagt taagagtctt attgaatcct ctgaactttt taacctccca gaagcattta
     2941 atcggggacc gaccgcccaa ccaatttcgt aagtagtaat ttcaatatct tttatacttg
     3001 aaacctcaaa taatttttta atccaaagcg gtggattact attaattact tctaaacctt
     3061 tttttacaat tattctatta aatcccgtta agggaattag gtcagtgacg gtagctacgg
     3121 aggcatttac tatcccgtcc ctgctttttg atcccaaagc ttttgataac aaccaagcta
     3181 ttgcggcacc acatacgcca tcgttccaca ccagaagatc gggggtgggc actttctccg
     3241 gcttttgatg atgatctgta agtatcacgg aatgacctaa ttttttgata taagaaattt
     3301 cttcaaatgc agtgacacct acatcgaccg taataaaaag cactttctca tcggatttat
     3361 aattgtccaa agcttcatct atcgcaagtt tggataaacc gtagttatgc tcaaatctat
     3421 tcggaataaa ataagaaact ttatcgtatt ttttttcata tttaaaaaag ttatacaaaa
     3481 tcgctgtcgc atttatcccg tcagaatcgt aatctccaaa aatcagaatg ggagtatttt
     3541 tttccatttc agaaaaaatt aggtcgcggg cttttgagag agatttttta aaatcagaac
     3601 ttaaagattc aaaagattca tttaaagtag gtgtttttaa gaacagatca atatctttaa
     3661 cacctcttcc ctcgaggata gagagcgaaa tatccttttt ctggttgtaa tcgcctgtga
     3721 ttttccatgt atacataatt tatataataa aaactaagaa aatggatatt tattttgaga
     3781 gttttaaata ctttttaagc accaatggta aaagtaccgt gtgagtaaaa aacactacca
//
LOCUS       DAOTFA010000045         3596 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate AMR_MDS_3115
            NODE_19461_length_3596_cov_9.256425, whole genome shotgun sequence.
ACCESSION   DAOTFA010000045 DAOTFA010000000
VERSION     DAOTFA010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868139
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (wastewater metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:36:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 536
            CDSs (total)                      :: 521
            Genes (coding)                    :: 520
            CDSs (with protein)               :: 520
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3596
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19461_length_3596_cov_9.256425"
                     /isolate="AMR_MDS_3115"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            235..1470
                     /locus_tag="P5014_02010"
     CDS             235..1470
                     /locus_tag="P5014_02010"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HRY56918.1"
                     /translation="MTKAKKIQKKESAMSKYLTDKDLQVKQLRVGDMIEGVVVSASHK
                     EILVDVGAKSEGIITGLELSEADKSYKSVQPGDKISAKISQIENAQGYIVLSLKKAEK
                     DKKWQDAEEAFEGGFVIDAVVLEYNKGGLLCDSLGLRGFIPLSHLDRVHFANDVAKFA
                     AGSEAELKESLKILSGKTLRVKVIELDKEKNRFVLSEKDALTTYSEESRKKRLSEIEI
                     GSSVEGIVTGIMPFGVFVDLDGVEGLVHISEIAWEKVNHPSNYFKVGQKIKVMVLGID
                     EASNKLALSVKKLIPNPWETVEEKYPVGTKVKGVVSKIVPFGAFVTLEKGLDGLIHIS
                     EAESPLEEGDKVSATVIQVDGANQKLALSTRDQSNIDKKDDKKKKEKEIVDDVEDFNE
                     DDLDESKEDSKKKISKKKK"
     gene            1510..2829
                     /gene="radA"
                     /locus_tag="P5014_02015"
     CDS             1510..2829
                     /gene="radA"
                     /locus_tag="P5014_02015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HRY56919.1"
                     /translation="MSKFKTVYTCKNCGYTSHQWVGQCPECNEWNSFVEDVISSKGSN
                     MSSSTRSNYEKPVKLSSVSSEPPKRISSGIGEFDRVLGGGFVPGQVVLLAGEPGIGKS
                     TILTEIAKNLKNTDVLYVCGEESAEQIKLRTTRMGYNADNLLVISESSAEAISDSIEK
                     TENIGLVIVDSIQTLFSEELLGSPGSLVQVRGCSQILTNSAKKAGIPMVLVGHVTKEG
                     VVAGPKVLEHIVDTILYLEGDSQHLFRILRTSKNRFGAVSEVGIFEMEESGIKEVKNP
                     SELFLKQRLPNASGSCVTVVMEGYRPLLFEIQALTVPTNFGYPRRTSSGFSNTRLQVL
                     IAILEKRAGLNLSNHDVYVNVAGGFKVSEYAVDLAVCLAIASSLKDKPIKDGVVAFGE
                     CGLNGEIRKVSHQEKRSGEAKKLGYGEVVSSDNTKNIFEAIKKSLAE"
     gene            2986..>3596
                     /locus_tag="P5014_02020"
     CDS             2986..>3596
                     /locus_tag="P5014_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56920.1"
                     /translation="MGNKSEEKQKLVVKKPSLRETKLLTRIIYAVIFFGLGFLLSRNL
                     FFEDLPLIGYHIALEVLTGAALAAFGFFIFPLVLMYVKYWIEALVVAAVSKIVTDFWD
                     QQSKRIQQARREKQRLKADEKSIREKQELDNAILLDTSVLIDGRVLDVVKAGFLDRPF
                     IVTQNVMNELQNLADNKNKVKRSRGRMGLDVVKELKKRTNVIVP"
BASE COUNT         1220 a          515 c          775 g         1086 t
ORIGIN      
        1 gcttgagcta tagcattaca tgctatagca tcaatctaaa tggtcgtcgc cgggacattg
       61 cttacaatat ctactttttt atattttacc cccagatatt tttcccttgt cttctcccca
      121 tcctttagat ataatcccac tgtccttaaa tggacggaaa gggggttttt atatcgtctt
      181 ggaaataacg gcataaagcc gtctcttttt ctttaaagat gataaaaacc tagaatgacg
      241 aaagctaaaa aaattcaaaa aaaagaatct gcaatgtcaa aatatttaac tgacaaagat
      301 ttgcaggtta agcagttacg tgtaggggat atgattgagg gagtagtggt gagtgctagt
      361 cacaaggaga ttcttgttga tgtaggtgct aaatcagagg gaataataac aggcttggag
      421 ctatccgaag ccgataagtc atataaatcg gttcaacccg gagacaagat atcagcaaaa
      481 atttcacaaa ttgaaaacgc acaaggatac atagtacttt cacttaaaaa agccgagaaa
      541 gataaaaaat ggcaggatgc ggaagaagct tttgaaggtg gttttgttat agacgctgtt
      601 gttttggaat acaataaagg aggccttctt tgcgacagtt taggtttaag aggctttatt
      661 ccactttcac atttagatag agttcatttt gctaacgatg ttgcaaaatt cgcagctggt
      721 agtgaagctg aattaaaaga atcattaaaa attctttcag gaaaaacttt aagagtaaaa
      781 gtaatagagc tcgataaaga aaagaataga tttgtcttgt ctgaaaaaga tgctttaaca
      841 acttacagcg aagagtcaag aaagaaaaga ttatcagaaa ttgagattgg tagttccgtt
      901 gaaggtattg ttacaggaat catgcctttt ggggttttcg ttgatcttga cggggtagaa
      961 ggacttgttc atattagtga gatagcttgg gaaaaagtta atcacccttc aaattatttt
     1021 aaagttggac aaaagattaa agtaatggtt ttaggaattg atgaggcttc taataaactt
     1081 gcattaagtg ttaaaaaact tatacctaat ccctgggaaa cagttgaaga gaaatatccc
     1141 gtaggtacaa aggtaaaagg ggttgttagc aaaatagtac catttggtgc ttttgtgacc
     1201 ttagaaaagg gtttagacgg tttaattcat atttcggagg cagagtctcc tcttgaagag
     1261 ggtgacaagg tttcagcaac tgttattcaa gttgacggag ccaatcagaa acttgcatta
     1321 agcacaagag atcaatccaa cattgataaa aaagatgata aaaagaagaa agaaaaagag
     1381 atagttgatg atgtcgaaga ttttaatgaa gatgacttgg atgaatcaaa agaggattcc
     1441 aaaaagaaga tctcaaagaa aaagaaataa ttaaactatt ttttaccttc tgtctgataa
     1501 gatatattta tgtctaaatt caagactgta tatacctgta aaaattgcgg atatacctca
     1561 catcagtggg taggccagtg tccggaatgt aacgaatgga attcatttgt tgaagatgtc
     1621 ataagttcca aaggttcaaa catgagttct tctaccagga gtaattatga aaaacctgta
     1681 aaactatctt ccgtatcctc tgaacctcct aaaagaattt cttcgggaat aggtgagttt
     1741 gatagggttt taggaggggg atttgtaccc ggccaagtgg tgcttttggc gggcgagccc
     1801 ggtatcggaa aaagtacaat ccttaccgag attgcaaaaa atcttaaaaa caccgatgtt
     1861 ttatatgtat gtggcgaaga aagtgcagaa caaattaaac tacgcaccac cagaatgggt
     1921 tacaacgctg ataatctttt agtaatttcc gaatcaagtg ccgaagccat ttcagatagt
     1981 attgaaaaaa ctgaaaacat cgggcttgtg atagtagatt caattcaaac cttgttttca
     2041 gaagaacttt taggttctcc gggatccctt gtacaagtga gaggttgctc tcaaatactg
     2101 acaaatagtg ccaaaaaggc tggaattcct atggtattgg tcggtcatgt tacaaaggag
     2161 ggggttgtag ccgggcctaa ggttttagaa cacatcgttg ataccatttt atatttagaa
     2221 ggagattcac agcatctttt tagaatatta agaaccagca aaaatagatt tggagctgtt
     2281 tcagaagtgg ggatttttga gatggaggag agtggtataa aagaagtaaa aaatccttcg
     2341 gaactttttt taaaacaaag attacctaat gcttctggtt cctgtgtaac cgtagttatg
     2401 gagggttacc gacctctttt atttgaaatt caagctttga cagtacctac aaatttcggt
     2461 tacccgagaa gaacttccag cggctttagc aataccagac ttcaagtttt gattgctatt
     2521 ttggaaaaac gtgcaggttt gaatttaagt aaccacgatg tttatgtaaa cgttgcggga
     2581 ggttttaaag tctctgaata cgcagtcgat ttagctgtct gcttagcgat agcatcttcc
     2641 ttaaaagata aaccaatcaa ggatggcgta gtagcttttg gagagtgcgg acttaacggt
     2701 gagataagaa aagtatctca tcaggaaaaa agatcgggtg aggcaaaaaa gctggggtat
     2761 ggggaagtcg ttagttcaga taataccaag aatatttttg aagcgataaa aaaatctctt
     2821 gcagaataaa ggtactctaa atttgctata aattttccat aattttttta taaaaccttt
     2881 gttacttttt atttttttaa aattgtaaaa tccctcgaac cgttttttaa ttttcacttc
     2941 ataaataatt ggtaatttag acttttttaa cttaatattt aagtggtggg aaataaatca
     3001 gaagaaaagc aaaaacttgt agttaaaaaa ccttctttga gggaaactaa attattaacc
     3061 cgaataatat atgcagtaat atttttcggt cttgggtttt tattatcgag aaatttattt
     3121 tttgaagatc tgcctttaat tggttatcac atagcactgg aggtattgac tggagcagct
     3181 ttggcggcat ttggtttttt catttttccc ttagttttaa tgtatgttaa atattggata
     3241 gaggcccttg tggtggctgc ggtttcaaaa atcgtaactg atttttggga tcaacagtca
     3301 aaaagaattc aacaagccag aagagagaag cagagattaa aagcagacga aaaaagtatt
     3361 agagagaaac aggaattgga caacgctatt ttactggata catcggtttt aattgacggg
     3421 agggttttgg atgtagttaa agcgggtttt ttggataggc cttttattgt tactcaaaat
     3481 gttatgaacg agcttcaaaa tttagcggat aacaagaata aagtaaaaag aagtagaggt
     3541 aggatggggc tggatgtggt aaaagagttg aaaaaaagga caaacgttat tgttcc
//
LOCUS       DAOTFA010000046         3517 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate AMR_MDS_3115
            NODE_20160_length_3517_cov_9.199307, whole genome shotgun sequence.
ACCESSION   DAOTFA010000046 DAOTFA010000000
VERSION     DAOTFA010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868139
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (wastewater metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:36:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 536
            CDSs (total)                      :: 521
            Genes (coding)                    :: 520
            CDSs (with protein)               :: 520
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3517
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20160_length_3517_cov_9.199307"
                     /isolate="AMR_MDS_3115"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2117)
                     /locus_tag="P5014_02025"
     CDS             complement(<1..2117)
                     /locus_tag="P5014_02025"
                     /inference="COORDINATES: protein motif:HMM:NF021897.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HRY56921.1"
                     /translation="MNPTDTTTINETLSLTVFIICVVALVAFIVLQIASWLRKKNEKK
                     NQFKLTFFQVLLPKDNEIETKAAEHMFSNLIGFKKGPFSAFFTGQYRISFEIVSKDSG
                     IAFYVAAPDEIATVVEKQINAAYPSAEIEIVTPPEIWDRGKYTKVAKLRLRGPEYFPI
                     KEYEDLKNDTMSSITNAMSKMKENQVLALQYVIQPATDSWRMAGRSFINRIKNANANP
                     EKKSSISDSFLEAVENKINNPGFYIKIRVISIAEDKIQAESQIQNLVAAFEQFTDISY
                     NRFVRKNAINTKKFVEDFIYRRIGVKRLVIPILGLKIKSNVSVLNNVEMATIFHFPNK
                     DITTPNLIWLGARKSAAPMNLPDENDGVFMGWSTFRGIKKKVFIRDEDRTRHVYIVGQ
                     TGSGKSQLMMWLALQDIKRGEGVAIIDPHGTDVNELLEKIPPERKEDVILFDAADRER
                     PLGLNMLEAKDETEKNMIINSFIALLYKLYDPNHQGIMGPLLERSIRNVMLTAMVDPE
                     ATMIDVLRMFIDEKYSKKFLDKVTDPLVRRYWTDEVANTTQNRKGETMGYFVSKFDRI
                     TTDNTMRNIIGQKKSSINFDEIMAQKKILLVDLAKGKLGEENSNFLGLLLVPRILTSA
                     LRRHQLKGDFPNFFLHVDEFQNFATPDFATILSEARKYKLNLTVAHQFINQLPEDIKT
                     AIFGNVGTTCAFRVGIDDAEYLET"
     gene            complement(2133..2543)
                     /locus_tag="P5014_02030"
     CDS             complement(2133..2543)
                     /locus_tag="P5014_02030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56922.1"
                     /translation="MPDTYNTPPVSSIFPVIPNGADVEETAEIVSKPLDTNSDENTII
                     PSPVYEALYGNSETTNTSTPFNESETSSITTITETPKLQTETDAIKSKPTTPTGEIPG
                     NQPVSTGATLLTIRSDEEEKRAIDGFTQKVPTII"
     gene            complement(2600..3106)
                     /locus_tag="P5014_02035"
     CDS             complement(2600..3106)
                     /locus_tag="P5014_02035"
                     /inference="COORDINATES: protein motif:HMM:NF024143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HRY56923.1"
                     /translation="MNMRTIDRKDSDFYRYGHPRHLCRNNGGSFSKGFFWGAVIGGIL
                     GVLFAPEKGEGTRKKIKEKAKDYEEKGKEALEKASEELEKARIKYEEAAKKVGPVIEK
                     AKEKIEEVKVKSEPYVEFAKEKASEVKEFIQENKDPIEDTLKDLANEILDEGKLIKKR
                     YFKGTRKR"
     gene            complement(3087..3368)
                     /locus_tag="P5014_02040"
     CDS             complement(3087..3368)
                     /locus_tag="P5014_02040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56924.1"
                     /translation="METLELILIVTRILVYIAVVGVLVYLVLILKEVREMVADAKDVV
                     KVGRNITTSIASPVNSVVGLLSGVTKGINAIRSVTDLFDKGEEDEYEDY"
BASE COUNT          943 a          663 c          591 g         1320 t
ORIGIN      
        1 gtttctaaat attctgcgtc atctatacca actctaaaag cacacgtagt tcccacgttt
       61 ccaaatatgg ctgttttaat gtcttctgga agttgattaa taaattggtg cgcaactgtg
      121 agatttaatt tatactttct tgcttcagat aagatagttg caaagtcggg tgtggcaaaa
      181 ttttgaaatt catccacatg aaggaaaaaa ttagggaagt ccccttttaa ttggtgtctt
      241 cttagggcag aggttagaat tcttgggact aaaagcaatc caaggaaatt tgagttttcc
      301 tcaccaagct ttcctttggc taaatctacc aaaagaatct ttttctgagc cattatttca
      361 tcaaaattaa ttgaagattt tttctggccg atgatgtttc tcatagtgtt gtctgtagta
      421 attcgatcaa attttgaaac aaaataaccc atcgtctcgc cctttctgtt ttgagttgtg
      481 ttggctactt cgtctgtcca gtatcttctt actaaaggat ccgttacttt atcgaggaat
      541 tttttggagt atttctcatc aataaacatc cttaatacat caatcattgt ggcttcaggg
      601 tctaccattg cagtaagcat tacgtttcta atagatcttt ccaagagagg tcccatgatt
      661 ccttggtggt tggggtcata tagtttgtaa agaagagcaa tgaaagagtt aattatcatg
      721 tttttttcag tctcgtcttt tgcttccagc atatttaatc caagaggtct ttctctgtca
      781 gcagcgtcaa aaagtatcac atcttctttt ctttccggtg gtattttttc caagagttcg
      841 tttacatctg taccgtgagg gtcaattatt gctaccccct cacctctctt tatatcttgt
      901 aaagcgagcc acatcatgag ttgtgatttt ccggaacctg tttgacctac tatgtacaca
      961 tgtcttgttc tgtcttcatc tctaataaat acttttttct ttattcctct aaaagtactc
     1021 cagcccataa agacaccgtc attttcatcc ggtagattca ttggtgctgc ggattttctt
     1081 gcacccagcc agattaggtt aggcgtagtt atgtctttat taggaaaatg aaatattgtt
     1141 gccatttcta cgttatttaa tactgaaaca ttggatttaa tttttaaacc taatataggg
     1201 attaccaatc ttttaacccc gattcttctg taaatgaagt cttcgacaaa ttttttagta
     1261 tttatggcat tttttcttac aaatcggtta taactaatgt ccgtgaattg ctcaaaagca
     1321 gctacaaggt tttgtatttg agattccgct tgaattttat cttcggcaat agaaattact
     1381 ctgattttta tataaaagcc ggggttgttg attttatttt ctaccgcttc taaaaaactg
     1441 tcgctgatac ttgatttttt ttcggggtta gcgttagcat tttttattct atttataaaa
     1501 cttcttccag ccattctcca gctatctgtt gctggttgta taacatactg aagtgcaagt
     1561 acttgatttt ctttcatctt actcattgca ttggttatgg aactcatagt gtcgtttttt
     1621 aaatcctcat attccttgat agggaagtat tctggtcctc taagtcttag ttttgctact
     1681 tttgtatatt ttcctctgtc ccatatctca ggaggagtga ctatttcaat ttctgctgac
     1741 gggtatgccg cgtttatttg cttttcaaca acggtagcta tttcgtcagg ggcagcaaca
     1801 taaaaagcaa ttcctgagtc tttagatact atttcaaaag atatcctgta ttggccagta
     1861 aaaaaagcac taaaaggtcc ttttttaaat cctattaaat ttgaaaacat atgttcggca
     1921 gcctttgttt ctatttcgtt gtctttaggg agaagaactt gaaaaaaagt gagtttgaat
     1981 tgatttttct tttcgttttt ctttcttaac cacgaggcga tttgtaggac tataaatgct
     2041 actagtgcca ccacacagat tataaaaact gtaagcgaga gtgtttcatt aattgtagtt
     2101 gtatcagttg ggttcatggg cagaaaaatt attcatataa ttgtaggaac tttttgtgta
     2161 aagccgtcga tagctctttt ctcttcctca tctgatcgga ttgttagcag agttgcgccc
     2221 gtactaacag gttggttgcc tggaatttct ccggttggcg tcgttggctt ggatttaata
     2281 gcgtctgtct ccgtttgtag tttaggagtt tctgtaatgg ttgttattga agatgtttcc
     2341 gattcattaa aaggcgtaga ggtatttgtt gtctcgctat ttccatacag ggcttcgtaa
     2401 acagggctcg gtattatcgt gttttcatca ctgtttgtgt caagaggttt agaaacgatt
     2461 tcagctgttt cttcaacatc ggccccatta ggaattacag gaaaaataga actaacaggt
     2521 ggtgtgttgt atgtatcggg catacaaata ttctaacatc ttagaggggg aaattaatag
     2581 cattgatcaa aaatgacaat tatctctttc tcgtaccttt gaagtatctt ttttttatta
     2641 attttccttc atccagtatc tcgtttgcta aatctttcag ggtgtcctca ataggatctt
     2701 tgttttcttg aataaattcc ttaacttcag atgccttttc tttagcaaat tcaacataag
     2761 gctctgattt aactttaacc tcttctattt tttcttttgc cttttcaata acagggccta
     2821 ctttttttgc ggcttcctca tattttattc tggctttttc taactcttcg gatgcttttt
     2881 ctaaagcttc ttttcctttt tcttcgtaat cttttgcttt ttcttttatc ttttttcttg
     2941 tgccctcacc cttttcaggt gcaaaaagaa ctcccaaaat acctccaatt actgcgcccc
     3001 aaaaaaatcc ttttgaaaac gaacctccat tattacggca cagatgtctt ggatgtccgt
     3061 atctgtaaaa atcagagtct tttctatcaa tagtcctcat attcatcctc ctctccttta
     3121 tcaaataaat ccgttacaga tctgattgca tttattcctt ttgtaacccc acttaaaaga
     3181 ccaaccacag agtttaccgg cgacgctatt gaagttgtaa tgtttctccc tacttttact
     3241 acatcttttg catcagcaac catttctctg acttctttta aaatcaaaac tagataaacc
     3301 aaaacaccca caacagcaat gtaaactaag atgcgggtaa cgattaatat taattctaaa
     3361 gtttccattt tgggctttct tgcccttatg aacaatacga aaactaaggg cagttaaatt
     3421 taattaaata taatcactat ttatgatgtc agtcaacctt tgatatactt taagcaataa
     3481 aaaattttaa aaagggaaaa agttatggac gacaata
//
LOCUS       DAOTFA010000047         3495 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate AMR_MDS_3115
            NODE_20360_length_3495_cov_6.615988, whole genome shotgun sequence.
ACCESSION   DAOTFA010000047 DAOTFA010000000
VERSION     DAOTFA010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868139
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (wastewater metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3495)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3495)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:36:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 536
            CDSs (total)                      :: 521
            Genes (coding)                    :: 520
            CDSs (with protein)               :: 520
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3495
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20360_length_3495_cov_6.615988"
                     /isolate="AMR_MDS_3115"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="P5014_02045"
     CDS             complement(<1..455)
                     /locus_tag="P5014_02045"
                     /inference="COORDINATES: protein motif:HMM:NF016553.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56925.1"
                     /translation="MKFSDFSIYMQRLEDTSKRLEMTDILTELIKKLSVDEIDKAIYL
                     ASGYVEAPFEEIKFNIAEKMMLKVIEQTFSTPQKRISAEEIQDVYRKTGDLGNVIFEF
                     KKNDKKFSDFSISEVHSKLLEIALIEGTGSQDSKITKISTLLKETDPLSA"
     gene            complement(519..1511)
                     /locus_tag="P5014_02050"
     CDS             complement(519..1511)
                     /locus_tag="P5014_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56926.1"
                     /translation="MYLPEYSITSKTLNNIAIIEYGKALIENTTILPSWESQLKKEAI
                     VKIVHGSLYLQGIVTDQQKIKAAVDDLNKSVNQEALNIIKATFLSNDISGNKELEEVD
                     IKYIHKILTESLIPKVKQGSYRSTKIPGKVDPEEILAQIVSLFDWYNSMDARETHRVV
                     TAGIMKACLENIQPFEEMNEIVSNLITYISLKTSGYGFKDLISLENYYKNTRARYKNE
                     IDTMDTEDSDLTTWIEYFTEGLASEVSTVSQNVKLLAKDTKIAKATGRTRVSPRQERI
                     IEYLQDYGVLQNKDFSTLFPDISEDTILRDLKVLVDMDIIQKLGSTKSSRYELK"
     gene            1606..1995
                     /locus_tag="P5014_02055"
     CDS             1606..1995
                     /locus_tag="P5014_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56927.1"
                     /translation="MDKERFEEYGFVSYGWKNRVFIVRIEIPLSEVRGFEKNFLKGRV
                     TLQAGGKVIGKACLKPLRLSLVYSEMSERDCLELVQYVVNHSIVIYEACIDVSPEECV
                     KTDTTFKVKIGNLIIGPTPVGVPVTLP"
     gene            complement(2042..3100)
                     /gene="recF"
                     /locus_tag="P5014_02060"
     CDS             complement(2042..3100)
                     /gene="recF"
                     /locus_tag="P5014_02060"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication and repair protein RecF"
                     /protein_id="HRY56928.1"
                     /translation="MKIKNLALINFRNHQDLKINFDDNTTMITGDNGSGKSTILESIY
                     ILSVGRLRTSKYDRDLIQYDKDFCTVRANIQTKEDDLEMELQIIKSEKFDNASTKKAK
                     INKVVKSIQYFTGVFNSVLFSPSDIQLITGSPSERRKYVDNILSQIDLEYKRSLNNYL
                     QAIRQRNKLLENINKGFGGQNEIGFYTDQILNHGKAIQEKRLKMFEDIGPIVSKYGKD
                     LNNKKTDLKINYRKNEISEERLNEYKSREIAAMTTLLGPHRDDFEILFDGHDISNFGS
                     RGEQRSCVLSLKIAEMEFIEKKKGDKPVLLLDDIFSELDGKHQEAVTNCIENNQTILT
                     STSTPNFLQDRELEVIKL"
     gene            complement(3107..>3495)
                     /locus_tag="P5014_02065"
     CDS             complement(3107..>3495)
                     /locus_tag="P5014_02065"
                     /inference="COORDINATES: protein motif:HMM:NF018533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HRY56929.1"
                     /translation="KSKKSTIKNVLLDQKIISGLGNIYATDTLFLSEVNPKTPTQDLT
                     LKQGADILKNSRLILKEGIKHRGSTLADKMYVDIFGNSGNQQNFFRIYGKNTCPVCNS
                     KTAFEKINGRGTYYCPTCQPLKTAKK"
BASE COUNT          936 a          574 c          611 g         1374 t
ORIGIN      
        1 gctgacagag ggtcagtttc tttcaaaagt gttgatattt ttgttatttt actgtcttgt
       61 gatccagtcc cttcaatcag ggcaatctct aaaagtttgg aatgaacttc tgatatagaa
      121 aaatcgctga attttttatc gtttttctta aattcaaata ttacatttcc taagtctcct
      181 gtttttctat acacatcctg aatttcttca gccgatatcc ttttctgggg agtagagaag
      241 gtctgttcta taacttttaa catcattttt tcggcaatgt tgaattttat ttcttcaaaa
      301 ggagcttcta catatcctga tgccaagtat attgctttat ctatctcatc cactgaaagt
      361 ttttttatca attcagttag gatatccgtc atttctaagc gtttggaggt atcttcgagt
      421 ctttgcatgt agattgaaaa atcactgaat ttcatagggc tattatagta tatattttgg
      481 attattatct ttttaaaaag gtattccttt agcgggattt atttcaattc gtaccttgaa
      541 gacttagtgc ttccgagctt ttgtattatg tccatatcaa ccaacacttt caaatctcta
      601 agtatagtat cctccgaaat atcagggaag agcgtggaga aatctttgtt ttgaagtact
      661 ccgtaatctt ggagatactc aattattcgt tcttggcggg gacttactct tgttcttccg
      721 gtagcttttg caatctttgt gtccttggct aataatttta cgttttgtga aacggttgaa
      781 acttcggagg cgagtccttc tgtaaaatat tctatccagg ttgttaaatc agaatcttcc
      841 gtatccatgg tatctatttc atttttatat cttgctctgg tgtttttata ataattttcc
      901 aatgatatta aatctttaaa tccatatccg gaagttttga gactgatgta agttatcaga
      961 ttagaaacta tttcattcat ttcttcgaac ggctgaatgt tttcaagaca ggctttcata
     1021 atacccgctg taactactct gtgtgtttct cttgcatcca tgctgttata ccagtcaaaa
     1081 agactgacta tttgagctaa tatttcttcc gggtcgacct tgcctggaat ttttgtactt
     1141 ctgtaagaac cctgctttac tttcgggatc aaactttcgg ttaatatttt gtgaatgtat
     1201 tttatatcta cttcttcgag ctctttgttt ccagatatat cgtttgaaag aaaggttgct
     1261 tttattatgt ttagagcttc ttggtttact gatttattta aatcatcaac cgcagccttt
     1321 attttttgtt gatctgttac tatcccttgg aggtacaaag atccatgaac gattttaact
     1381 attgcctctt tttttagttg tgattcccaa gatgggagta tagtggtgtt ttcaattaaa
     1441 gccttgccat attcaataat ggcgatgttg tttagtgttt tagaggttat tgaatattca
     1501 ggcaggtaca tatgattaat tctcgcatat ttcccgcaaa atgaaaataa ggttgcgtgt
     1561 tgctggtata atgtttttaa aactagatag aaagaagagg agattatgga taaagaacgt
     1621 tttgaagagt atggttttgt atcgtatggt tggaaaaatc gtgtttttat tgttcgtatt
     1681 gagatccctt tatcagaagt aagaggtttt gaaaaaaact ttttaaaggg aagggtaact
     1741 ttacaggcag gtggaaaggt tattggcaag gcatgtttga aacccttgcg tttatcgctt
     1801 gtgtattcag agatgtctga gcgggactgt ttggaacttg tgcaatatgt ggtcaatcat
     1861 tcaatagtga tttacgaggc ttgtattgat gtctctccag aagaatgtgt caaaactgat
     1921 acaactttta aagtaaaaat cggcaatctg ataatcggcc caacccctgt cggtgttcct
     1981 gtaaccctgc cttaacaaga acagaccccc gtgatttgaa aaatgatcgg gggttgtctt
     2041 tttatagctt aattacttca agctctctat cttgtaaaaa gttgggggtg gaggtgcttg
     2101 ttaatattgt ttgattgttt tctatacagt ttgttacggc ttcctggtgt tttccatcaa
     2161 gttctgaaaa aatatcatcc agaagaagta caggtttatc gcctttcttt ttttcaataa
     2221 attccatctc tgcaattttt agtgatagta cacagcttct ttgttctcct ctggaaccaa
     2281 aatttgaaat atcgtgccca tcaaacagta tttcaaaatc atctctgtga ggacctaata
     2341 atgttgtcat agcggctatt tcacgacttt tatattcatt aagtctttct tcgctaatct
     2401 cattttttct gtaatttatc ttgaggtcag tttttttatt gtttagatct ttgccgtatt
     2461 ttgaaacaat tggtccgata tcttcaaaca tttttaatct tttttcctga atagctttgc
     2521 catgatttaa aatttgatca gtgtaaaaac caatttcatt ttgccctccg aaacccttat
     2581 ttatattttc caataattta tttctctgtc taattgcctg taaataatta ttcaaacttc
     2641 ttttatattc caaatcaatc tgagaaagaa tattatcgac gtatttcctt ctttcagaag
     2701 gagaacctgt tataagctgg atatcgctgg gggagaagag tacagaattg aaaactcctg
     2761 tgaagtattg aatgcttttt acaactttat ttattttggc ttttttagtt gaagcattat
     2821 caaatttttc actttttatg atctgtaatt ccatctctaa atcgtcttct tttgtttgga
     2881 tattagccct aactgtgcag aaatccttat cgtattgtat aaggtctcta tcatattttg
     2941 atgttcttag tctacctaca gaaagtatat aaatagattc taaaatggta gattttcccg
     3001 agccgttatc gccggttatc atagttgtat tatcgtcaaa atttatttta agatcttgat
     3061 gatttctgaa atttattaaa gctaagtttt ttattttcat ctctatttat ttttttgccg
     3121 tctttaatgg ctgacaagta gggcagtagt aagttcccct tccgttaatt ttttcaaatg
     3181 cggtttttga gttacaaacc ggacaagtat tttttccgta aattcgaaaa aagttttgtt
     3241 gatttccact gttcccaaaa atatctacat acattttatc tgccagagta gaccctctgt
     3301 gtttaattcc ctcctttaaa attaagcgag aattttttaa tatatctgca ccctgtttta
     3361 gggtcaaatc ttgggtagga gttttgggat ttacttccga taggaagagt gtgtcggtag
     3421 cataaatgtt tccaagccct gagattattt tttgatctaa aagtacgttc ttgattgtag
     3481 attttttgct tttta
//
LOCUS       DAOTFA010000048         3377 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate AMR_MDS_3115
            NODE_21419_length_3377_cov_8.388621, whole genome shotgun sequence.
ACCESSION   DAOTFA010000048 DAOTFA010000000
VERSION     DAOTFA010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868139
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (wastewater metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3377)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3377)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:36:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 536
            CDSs (total)                      :: 521
            Genes (coding)                    :: 520
            CDSs (with protein)               :: 520
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3377
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21419_length_3377_cov_8.388621"
                     /isolate="AMR_MDS_3115"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..283
                     /locus_tag="P5014_02070"
     CDS             <1..283
                     /locus_tag="P5014_02070"
                     /inference="COORDINATES: protein motif:HMM:NF012265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="putative dsRNA-binding protein"
                     /protein_id="HRY56930.1"
                     /translation="CKEFVERELLYKIDEVIKKEKYKDAKSVFQEKAQEKFSVTPTYK
                     IEDSWGPDHEKNFKVGVFIGDKLWGEGQGKSKQKAEQNAAEKALEKIDN"
     gene            311..808
                     /gene="rpsP"
                     /locus_tag="P5014_02075"
     CDS             311..808
                     /gene="rpsP"
                     /locus_tag="P5014_02075"
                     /inference="COORDINATES: protein motif:HMM:TIGR00002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HRY56931.1"
                     /translation="MSLTIRLSRIGRKNQPAYKLVVSNTRDKRNGKFVDILGHFNPLQ
                     PENKFSYDKEKYEKWVKSGALITEAVKKLMENKYEYIKYDPKKIKEEKENAKKMAEEK
                     AKEVKPENKTEEVKPETEGPEEKPAEEVKPEAEDENIENDGETPAEESVEEPAEEVQE
                     EAKGE"
     gene            810..1061
                     /locus_tag="P5014_02080"
     CDS             810..1061
                     /locus_tag="P5014_02080"
                     /inference="COORDINATES: protein motif:HMM:NF024483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HRY56932.1"
                     /translation="MRDFLEFLIKEIVSKPEEVEVTENNDGEIFIYNVHVSNEDMGVV
                     IGKEGKNIKALRNIAKAKAIKDNVRIQIVLEENTESSEN"
     gene            1068..1787
                     /gene="trmD"
                     /locus_tag="P5014_02085"
     CDS             1068..1787
                     /gene="trmD"
                     /locus_tag="P5014_02085"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: protein motif:HMM:TIGR00088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HRY56933.1"
                     /translation="MIKFDIVTLFPTLFIEHFNNLPFKKAVSKKLAEYNLWDLKNYSI
                     NNYGSVDDKTYGGGVGMVIMIEPIFKALSDIYSEKIMEDRIKGNSDFFINNNHKIIVL
                     SPRGERFNQKKARELSKATQITLICGRYEGLDARIEDNIATDVISLGDFVLSGGEIPA
                     LAVMESITRLLPGVLDKEEASKIESFSFEDGVGNEFIEYPQYTRPENFKGLEVPKVLL
                     SGNHKEIEKWRKENSKTFKSD"
     gene            complement(1893..2783)
                     /gene="pilO"
                     /locus_tag="P5014_02090"
     CDS             complement(1893..2783)
                     /gene="pilO"
                     /locus_tag="P5014_02090"
                     /inference="COORDINATES: protein motif:HMM:NF016253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA];
                     GO_process: GO:0043683 - type IV pilus assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 4a pilus biogenesis protein PilO"
                     /protein_id="HRY56934.1"
                     /translation="MMPEENKAKKEASMLIYKIKDTVLNFLIPLIAVVGTVLLLILYI
                     MPSYKSLPVKKQELQGKVTLKNTLTNKVSDLNRLVDYKSVFDENSDIVNKVLVSEPEV
                     PRLLDQASQLAEKSGASLEKLSFSYSSKGNSSTGLDTVSVSMGINSSFEQLILLMELI
                     ENASRFVSVPSFRYSVSDKTTSEKAAVSSTFSVDSPYLFVQSSAVTDDPIRIDVTSNE
                     FLDFMSMLKGLDYYDFINKNIQAEEEKPEEAKEEEVEPVNETEKNTNTETPAAPTTPV
                     ENPTPVAPQNTDGGSVFPTQ"
     gene            complement(2780..3361)
                     /locus_tag="P5014_02095"
     CDS             complement(2780..3361)
                     /locus_tag="P5014_02095"
                     /inference="COORDINATES: protein motif:HMM:NF016992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HRY56935.1"
                     /translation="MLNDINLIPQTEVIEQKKSKVLASSSVFSVLLLLIALGVSAYFF
                     TTLSKVNSEIKKTDSEIESLRSKIKSMSEVEIAARNLDKRYTALKNLFTGRSKYSLLL
                     RELEARKPSDVSIQNSDIRPGQISISGTSGSYISLKSFMDNLLNKEFLDGNAELKDLF
                     TTLSLNSVSLDKSNNSVKFFIVLTYQDGRLQGL"
BASE COUNT         1269 a          525 c          602 g          981 t
ORIGIN      
        1 ttgtaaggaa tttgttgaaa gagagcttct ttataaaatt gatgaggtga ttaaaaaaga
       61 aaaatataaa gatgcaaaat cagtctttca ggaaaaagct caagaaaaat tcagtgttac
      121 cccaacgtat aaaattgaag actcctgggg acctgaccac gagaaaaatt ttaaggtagg
      181 agtttttata ggagataaac tgtggggaga aggacaaggt aagagtaagc aaaaagcaga
      241 acagaatgca gctgaaaaag ccttagaaaa aatagacaat taattaccct tctgctacaa
      301 tcaatcagct atgtcactaa caataagatt atcgagaata ggaagaaaaa atcaaccggc
      361 ttataagctg gtagtttcta atactaggga taaaagaaat ggaaaatttg ttgatatttt
      421 aggtcatttc aatcctttac aacccgaaaa taaattttca tatgataaag aaaaatatga
      481 aaaatgggtt aaaagcggag ctctcatcac agaggcagta aaaaaattaa tggaaaataa
      541 atacgaatat ataaagtacg atcctaaaaa gataaaggaa gaaaaggaaa atgctaaaaa
      601 aatggctgag gagaaagcaa aagaagtaaa gcctgaaaat aaaaccgaag aagttaaacc
      661 cgaaactgaa ggaccagaag aaaaaccagc ggaagaagta aaaccagagg ctgaagatga
      721 aaatatagaa aatgacgggg aaacacctgc agaagaatcg gtggaagaac cggcagaaga
      781 agttcaagaa gaagccaaag gagaataaaa tgagagattt tctggaattt ttaataaaag
      841 aaatcgtttc aaaacccgaa gaagtagaag taactgaaaa taatgacgga gaaatcttca
      901 tttataatgt acacgtttca aatgaggata tgggtgttgt aataggaaaa gaaggtaaga
      961 acattaaggc tttaagaaat attgccaaag ctaaagctat taaagacaat gtaagaattc
     1021 aaattgtctt ggaagaaaat acagagtctt ccgaaaatta attttaaatg attaagtttg
     1081 atatagttac actttttccc acacttttta ttgaacattt taataacctc ccctttaaaa
     1141 aggcagtttc aaaaaaactt gcagaatata atctttggga tttaaaaaac tattctatta
     1201 ataattacgg ctcggttgat gataaaacct acggaggcgg ggtagggatg gttatcatga
     1261 tagaacctat ttttaaagcc ctttcagata tttatagtga aaaaataatg gaagatagaa
     1321 taaaaggtaa ttccgatttt tttataaata ataatcataa aattatagtg ctgtccccaa
     1381 gaggagagag atttaaccag aaaaaagcaa gagaattatc aaaagcgacc caaattacgc
     1441 tcatttgtgg gcgatatgaa ggtcttgacg caagaattga ggataatatc gcaacagacg
     1501 taatctcctt aggagacttt gtattaagcg ggggagaaat cccagcttta gcagtaatgg
     1561 aaagtattac aagactgtta ccgggagtat tggataaaga agaagcttca aaaatcgaaa
     1621 gtttttcttt tgaagacggg gttgggaatg aatttattga gtacccacaa tacacaagac
     1681 ctgaaaactt taagggttta gaagtaccca aagttcttct atctggaaat cacaaggaaa
     1741 tagagaagtg gagaaaagaa aattccaaaa ctttcaagag tgattaaaaa aacattaaac
     1801 gttacttttt taatacaaaa actattcaaa aatccacaga tatagaaacc acttttcagt
     1861 actaatgtcg ccatggatta taaaatttaa gtttattgag ttgggaaaac agaacctccg
     1921 tcggtatttt gcggtgcaac aggtgttggg ttttcaacag gtgttgtcgg agcagcaggg
     1981 gtctccgtat ttgtattctt ttcagtctcg ttaaccggct caacctcttc ttccttagct
     2041 tcttcgggtt tttcttcttc tgcttgaata tttttattaa tgaaatcgta ataatccaat
     2101 cctttcagca tactcataaa atctaagaac tcattactag taacatctat tcttattgga
     2161 tcatcggtaa cagcagagga ttgtacaaat aaataaggag aatcaactga aaaagtgcta
     2221 gaaaccgcag ctttttcaga tgttgttttg tcagaaactg aatacctaaa gcttggcacg
     2281 ctgacaaatc ttgaagcatt ttcaattaac tccatcaaca atatcaactg ttcaaaactt
     2341 gaattgatac ccatagaaac gctcactgta tccaaacctg ttgaagaatt tcctttagaa
     2401 ctgtaggaaa aactaagctt ttccaaagag gctcctgatt tttcagctag ctgggaggct
     2461 tgatctaata atcttggtac ttcaggttcc gataccaata ctttatttac aatatcagaa
     2521 ttctcatcaa aaacactttt ataatcgacc agcctgttta aatcagatac tttgtttgtc
     2581 aaagtatttt ttaaggtaac ttttccctga agttcctgtt tctttaccgg aaggctttta
     2641 tacgaaggca taatgtacag tatcaaaagc aacaccgtac ctactactgc tattaaagga
     2701 ataagaaaat ttaaaacagt gtcctttatc ttataaatca acatacttgc ttctttttta
     2761 gctttatttt cttctggcat catagacctt gcaacctccc atcctgatat gtcaaaacta
     2821 taaaaaactt cacactatta ttagatttat ctaaactaac ggaattcaaa gaaagagttg
     2881 taaaaagatc tttcaattcg gcgttaccat cgagaaattc tttattaagt aaattatcca
     2941 taaaactttt aagtgagatg tagcttccgg aagttccgga tatactaatt tgcccgggac
     3001 gaatatctga gttttgaata gaaacatcag atggttttct tgcctcaagt tctcttaaaa
     3061 gaagtgaata ttttgatctc cctgtgaata aattctttaa agcagtgtac cttttatcta
     3121 aattcctggc ggcaatctct acttctgaca ttgacttaat ttttgatctc aaagattcta
     3181 tttcagaatc tgtctttttg atttcagagt taactttaga aagagtagta aaaaaatatg
     3241 cagaaacccc cagggcaata agtaataaaa gtactgaaaa aacagagctc gaagccaaaa
     3301 ctttagattt tttttgctct attacttcag tttgcggaat taaattaata tcatttaaca
     3361 tttttgactc ctctcaa
//
LOCUS       DAOTFA010000049         3223 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate AMR_MDS_3115
            NODE_22964_length_3223_cov_5.503788, whole genome shotgun sequence.
ACCESSION   DAOTFA010000049 DAOTFA010000000
VERSION     DAOTFA010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868139
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (wastewater metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3223)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3223)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:36:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 536
            CDSs (total)                      :: 521
            Genes (coding)                    :: 520
            CDSs (with protein)               :: 520
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3223
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22964_length_3223_cov_5.503788"
                     /isolate="AMR_MDS_3115"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1205
                     /locus_tag="P5014_02100"
     CDS             <1..1205
                     /locus_tag="P5014_02100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008439845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HRY56936.1"
                     /translation="GNHEFDYGLPHLLFLEKVANFPIVNANLYVKKYNKRLMNPYKII
                     NKDGFDILFTGILTEKVIDSLKMDKLVGSFVTLEEASNEVGKICNAYKNDDIDLTILL
                     THIGFESDKELAAMLKPEWGVDMIIGGHSHTFLEKPEVVNNILISQAGVGSDQIGRYD
                     ITVDDETNSIVDYKWQLIPINDNIAEPDEELTKFIESFREEVDRKYNTIVTKFSKKLT
                     HPKREGETELGNLIADAFAEISQSDVMMVGSGSIRVKELGPIVTLKDLKACFPYDDSL
                     NRFKIRGDQLKRIFNHVMRIENRDGEGECYEVNLNVKAVYNENEKKLESLKIRDSDVM
                     DNFLYSICIQGYHLQNCQAYLNISEKELLESGQNKVISTSAAEVLEEWMRNNQNVSRE
                     VEGRLVFK"
     gene            complement(1235..1438)
                     /locus_tag="P5014_02105"
     CDS             complement(1235..1438)
                     /locus_tag="P5014_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56937.1"
                     /translation="MRGQRIRFSSFLVPPLPPWIFIKYGKYIKLKHWANTGYYGSGGS
                     IKAAIEYIEKKVKEVEEDREGNG"
     gene            1604..2743
                     /locus_tag="P5014_02110"
     CDS             1604..2743
                     /locus_tag="P5014_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56938.1"
                     /translation="MKFDKIKSWKKIPRIHFIISLMLIFFVWFFLALYNEEIPGSEDF
                     IGFSSVLVGLYFLIIVIPWVFIPRPIVLKVYLTIVSIFLTLMLGRFLHTFAGISTVRP
                     LEIFGLSFGILTLSLITLMPIILTVLLIIFNKTNVKSNYIPMFIYPLLIFLTLNHIVK
                     VSSTTFMQSLLVAVREKKYPYEDSQSVFFSKDMTIKKLESELQKNQDIINRLKSELDR
                     DACESWEHVRTFSVEVTDEYVIEESEFGVIVVKKSSSPWMAVTEASIYISEVPCVCIN
                     SEGGDIYNYKRSVYDMLESYEIGPHYRINKGNYTREGNIIIDGVEVKVFKNEEVYESP
                     PGVIEIRYQFNKNGVRYIAGAYFSDKEGGISLSEAQEILSTIKIN"
     gene            complement(2984..3060)
                     /locus_tag="P5014_02115"
     tRNA            complement(2984..3060)
                     /locus_tag="P5014_02115"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3024..3026),aa:Pro,seq:ggg)
BASE COUNT         1033 a          510 c          602 g         1078 t
ORIGIN      
        1 taggaaatca tgagtttgat tacggacttc ctcatctttt atttttagaa aaagtggcaa
       61 atttcccgat agttaacgca aatctttacg ttaaaaaata caacaaacgc ttgatgaacc
      121 cttataaaat aataaataaa gatggttttg atattctctt tacgggaatt ttaactgaaa
      181 aggtcattga ttctttgaaa atggataaat tggttggtag ttttgtgact ttggaagaag
      241 caagcaacga agttggaaag atttgtaatg catataaaaa cgacgatatt gatttaacca
      301 ttctacttac tcatattggt tttgagtcgg ataaagagtt ggctgcaatg ttaaaacctg
      361 aatggggagt tgatatgata atcggaggtc attcccacac gtttttagaa aaacctgaag
      421 tagtgaataa cattttaatt tctcaagccg gggtgggctc tgatcaaatt ggtcgttacg
      481 atattactgt tgatgatgaa acaaatagca ttgtggatta taaatggcag ctaattccta
      541 taaacgataa cattgctgaa cctgatgaag aactaactaa atttatcgaa tctttcagag
      601 aagaagtaga tagaaaatat aataccatcg ttaccaaatt ttcaaaaaaa cttacacatc
      661 caaaaagaga gggagagacg gagttgggca atcttatcgc cgatgctttt gcagaaattt
      721 ctcaaagtga tgtaatgatg gttggaagcg ggtctataag agttaaagag ttgggtccaa
      781 ttgtgacttt aaaagattta aaagcctgtt ttccttatga tgacagttta aatagattta
      841 aaattagagg agatcagtta aaaagaatat tcaaccacgt tatgagaata gaaaacaggg
      901 atggggaagg tgagtgttat gaggtaaatt taaatgttaa agccgtttat aatgaaaatg
      961 aaaaaaagtt ggaatctttg aaaatccgcg actccgatgt tatggataat tttctgtact
     1021 caatatgtat tcaaggctat catctacaaa attgtcaagc ttacttaaat ataagtgaaa
     1081 aagagctttt agaatcaggt caaaacaaag ttatatcgac ttctgcggct gaggttttag
     1141 aggagtggat gagaaacaac cagaatgtta gcagagaagt tgagggtagg ttggttttta
     1201 aataaaattt agagagcgtt tggatttaga ttttttaccc attaccctct ctatcttctt
     1261 caacttcttt tacctttttt tcaatatatt ctatggctgc tttaatactt cctcccgaac
     1321 cgtaataacc tgtattcgcc caatgtttta gttttatgta cttgccgtac ttaataaata
     1381 tccatggggg caggggtggt actaaaaaac ttgaaaatct tattctttgg cctctcattt
     1441 cctcctcctt tgttgtattg atttcagtat acaatatggt caaataacaa tatattgata
     1501 caagttctaa tttttagaag atacaaacag caaccttatt tttaaagacg tctaaaaatt
     1561 ttaatagctt actgaactta tgagatgcta taattttatt aggatgaaat tcgataaaat
     1621 taaatcatgg aagaaaatcc caaggataca ctttattatt tcattaatgc taattttttt
     1681 tgtttggttt tttctggctt tatacaatga ggaaattcca ggttcggaag attttattgg
     1741 attttcgtct gtgttagttg gtctttattt tttaattatt gtaataccgt gggtgtttat
     1801 tccaaggccg atagtcttaa aggtttattt aacaatcgtt tcaatatttt taacgcttat
     1861 gttaggtaga tttttacaca cttttgcggg gattagcact gtcaggcctt tagaaatatt
     1921 cggtttgtct tttgggatac taaccctatc tttaattaca ttaatgccaa taattttaac
     1981 tgtacttcta ataatattta acaaaaccaa tgtaaaatcg aattatattc cgatgtttat
     2041 atatcctctt ttgatatttc taactttaaa tcacatcgta aaagtttctt ccacgacatt
     2101 tatgcaatca ttgttggtgg cggtcagaga aaaaaaatat ccgtatgaag attctcaatc
     2161 tgtctttttc tcgaaagata tgactattaa aaaattagaa tccgaattac aaaagaatca
     2221 ggatattata aatagattaa agtcagaatt agatagagac gcctgcgaat cctgggagca
     2281 tgttcgtaca ttctcagtag aagtaacaga cgaatacgtt atagaggaat ccgaattcgg
     2341 ggttatcgtt gtaaaaaaga gttcttctcc atggatggcg gttaccgagg cctctattta
     2401 catttcagaa gtaccttgcg tgtgtattaa tagtgagggt ggagatatct acaattataa
     2461 aaggtctgtt tacgatatgc tggaatctta tgaaataggg cctcattatc gtattaacaa
     2521 aggtaattat acaagagagg gtaatattat catcgatggt gtagaagtaa aagtttttaa
     2581 aaacgaggaa gtttatgaat cccccccagg tgttattgag attaggtatc aatttaataa
     2641 aaacggtgtg cgatatattg caggtgcata cttctcagat aaagagggtg ggataagttt
     2701 atctgaggcg caagaaattt tatccacgat taagattaat taaattttag atacttcttg
     2761 tcagaaaaaa ctgccaatca aacctaaagg tttgcttgcc cgtttactgg tcggggtgac
     2821 aggactcggt cacgtgtctt aagacactct aactcatatt cattcgtaag agtctctaaa
     2881 gctcgcgacc ccgcctcctt cgtcacttcg ttcctcagtc cggcattctc gtcctgtcaa
     2941 aacagaaaga tagttaccac ccttcggatg atacctaact ttctggtcgg ggtgacagga
     3001 ctcgaacctg cggcctctcg caccccatgc gaacgcgcta gccaactgcg ccacaccccg
     3061 acgacagagt tattttatca ccttaactac cattttgtat ttggaatatt taaaaaaagg
     3121 cggccaaagt atttctttaa ccgccattag ctttattact tgctatttag tttttttgcc
     3181 ttgcttttat taataaactc tcttactttt gttcctacgt ctt
//
LOCUS       DAOTFA010000050         3139 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate AMR_MDS_3115
            NODE_23963_length_3139_cov_8.680285, whole genome shotgun sequence.
ACCESSION   DAOTFA010000050 DAOTFA010000000
VERSION     DAOTFA010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868139
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (wastewater metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:36:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 536
            CDSs (total)                      :: 521
            Genes (coding)                    :: 520
            CDSs (with protein)               :: 520
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3139
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23963_length_3139_cov_8.680285"
                     /isolate="AMR_MDS_3115"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(382..1254)
                     /locus_tag="P5014_02120"
     CDS             complement(382..1254)
                     /locus_tag="P5014_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56939.1"
                     /translation="MKNNRLFYFLFLSITFPTYGQDSTFIKINEWFTENKFAIRQTFD
                     GSKNENKPASFTYQEDHESPNDFLIVDIALKISELELLKNSNAVLIFYPKIEWHKSTN
                     ETDKKNKLDGGINFEYYPLGLEKNAIVPWFQGTSSFKRNFIDEVYEKKLAFQLSLSGK
                     KQFLPGSNIRDSKDNFRARYYPYVGIEYNHLPDLISKGVTEEFSAYFLRFFAETWILP
                     QTLQFNLDATYREILSTDTSLRKSLPILISSLYLYPGKQESFGIGFEYKYGYDNTAKF
                     QLIQISSLKLSWKI"
     gene            complement(1251..1514)
                     /locus_tag="P5014_02125"
     CDS             complement(1251..1514)
                     /locus_tag="P5014_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56940.1"
                     /translation="MKETIKTNVIAYICSYANMKPKNIKEEYILKNHPIKLDDTQLAL
                     LAMALRAYVKSLNPKETVLATELRKKDLDVKKTYELIIKKAGI"
     gene            complement(1563..2822)
                     /locus_tag="P5014_02130"
     CDS             complement(1563..2822)
                     /locus_tag="P5014_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56941.1"
                     /translation="MSLGFKYAAKNEAPIYESVVNGNGKKIVNRILMGTYVTVVEDLG
                     DFLKVFTVGTDGWMKKSDLSDHMGLKIFYLDVGQGDGVLIEVGNIRILVDAGPGKNMH
                     GYLTKFQYTYLIKAGKKVHIDYVFVSHFDLDHYKGFIEIMKNEHFTFGTIYHSGILKF
                     ASSSTYNTGLGNVTGSGSSKLLTTYFDDLLTHTETNAFNRDITNFMEQLVAMQSSGRI
                     STTKRLGAGETPLDLLIENKTFRIEALGPIMETTPSGKGFKYFTNDDGKTINGHSIIL
                     KLTFGQRTFLLGGDLNSSSEKYLMEKYHPSNPFEVDLAKSCHHGSSDFEIDFMKKINP
                     FATVISSGDNEGHAHPRADAIGCAGKYSRGERPLVFSTELARSVDMRTRKILFGMINV
                     RCNGDEIHISQMKEVKKYEDLWDSYAI"
BASE COUNT          988 a          559 c          542 g         1050 t
ORIGIN      
        1 cttcgcccta ctaggacaag cgtagcaaaa caacgaagga aggaacggca gcaaccctca
       61 tagccgagga cgtgcctcgc cgcaggtggg ggaacgacga gcgacttggg ttgcggagag
      121 actgacgagg tagttttcaa gcttgggcta gtgaggcttg atttttttgt ttcttttttc
      181 atcaaggaaa aaagaaaaga acatcaaatg aaaaccaact aaccacaaac aaaactcagt
      241 gtctcagcat cttggcccct caaaaaaaaa aaccccacga agaagtaggg gggtataaaa
      301 atcaagtcgt tcttaatcca tattttttac ctatgtagtt acctttcatt agcaagtggg
      361 ggtaattata gggattcctt tttatatttt ccaagataat ttgagagaag aaatttgaat
      421 caattgaaat ttagcggtgt tatcatagcc gtatttatat tcaaatccaa taccaaaaga
      481 ttcttgtttt cccgggtata gataaagtga gcttataaga attggcagtg attttctcaa
      541 agaagtatca gtagatagaa tttcccgata ggtagcatca agattgaatt gcaaggtttg
      601 tggtagaatc caagtttcag caaagaacct taaaaagtag gcagaaaact cttccgtaac
      661 tccttttgaa atcaaatcgg gcaaatggtt atattcgata ccaacataag gatagtatct
      721 tgctctgaaa ttatcttttg aatctcttat gtttgatcca gggagaaatt gtttttttcc
      781 cgaaagggat aattgaaagg caagtttttt ttcgtaaact tcatcgataa agtttctttt
      841 aaaagaagac gttccttgga accaaggtac tattgcgttt ttttccaagc ctaatgggta
      901 gtattcaaaa ttaatacctc catcgagttt gtttttttta tcagtttcgt tggttgattt
      961 atgccattct atttttggat aaaaaatcaa aacagcattg gagtttttaa gcaactccag
     1021 ttcagaaatt tttaacgcta tatcaacaat caagaaatca ttagggctct catggtcttc
     1081 ttgataggtg aaagaggcgg gtttgttttc attttttgaa ccgtcaaagg tttgcctgat
     1141 ggcaaattta ttttcagtaa accattcatt tatttttatg aaggtggagt cttgtccata
     1201 agttgggaag gttatactta aaaacaaaaa gtagaataaa cggttatttt tcatatgcca
     1261 gcttttttga ttattaactc ataggttttc ttaacatcta agtctttttt tcttaattcc
     1321 gttgctaaaa cggtttcttt tggatttaaa ctttttacat atgctctgag agccattgct
     1381 aataaagcaa gttgagtgtc atctagttta atgggatgat tttttaagat atactcctct
     1441 ttaatatttt ttggtttcat attggcgtag gaacagatat aggctattac gtttgttttt
     1501 atcgtttctt tcatttttag ttgtattagt ttacttaata ttataccctt gttttactaa
     1561 attcaaattg catacgaatc ccataaatct tcatattttt ttacttcttt catttgactg
     1621 atatgaattt catctccgtt acaccgcaca ttgatcattc caaaaagaat tttacgggtt
     1681 cgcatatcaa ctgacctggc caattctgta gaaaacacca aggggcgttc accgcgggaa
     1741 tacttgccgg cacaacctat cgcatcggct cgggggtggg catgaccctc attgtctccg
     1801 gacgagataa ccgttgcaaa ggggtttatt tttttcataa aatcaatttc aaaatcggaa
     1861 gaaccatgat gacaggattt ggccaaatct acttcaaatg gattcgaagg atggtatttt
     1921 tccatgaggt acttctctga agaactattc aaatcgcccc ccaataaaaa tgtgcgttga
     1981 ccgaaagtta gtttcaaaat aatactatgc ccgttaattg tttttccatc atcgtttgta
     2041 aaatacttga atccttttcc acttggggta gtttccatta taggtcctaa agcctcaatt
     2101 cggaaagttt tattctcaat gagtaaatca agaggagttt ctccggctcc caaacgttta
     2161 gtagtcgata ttcttcctga agattgcata gcaaccaact gttccataaa attagtaatg
     2221 tctcggttaa aggcgttagt ttcagtatgt gtaagtaaat cgtcaaaata ggtagttagt
     2281 aattttgaag aacctgaccc agttacgttt cctaatcctg tgttataagt actggaagat
     2341 gcaaatttta aaatgcccga atgataaatg gttccaaatg taaaatgttc atttttcatg
     2401 atttcaataa atcctttata atggtcaaga tcaaaatgac tgacaaaaac atagtcaatg
     2461 tgtacttttt taccggcttt tattaaatag gtatattgaa acttggtcag atacccatgc
     2521 atgttttttc cggggccggc atcaaccaat attcttatat tccccacctc tatcaaaaca
     2581 ccatcacctt gccccacatc taaataaaat atttttagcc ccatgtggtc ggataaatcg
     2641 gattttttca tccaaccatc tgtgccaacg gtaaaaactt tcaaaaaatc acccaaatct
     2701 tctaccacgg taacatatgt tcccataaga atacggttga caattttttt tccatttccg
     2761 tttactacac tttcataaat aggtgcttca ttttttgctg catatttaaa acctaaactc
     2821 ataacaataa gattttataa ttagttgtta aaaatatcaa atcaacttcg ataaataaat
     2881 ccctacaaat aggtattttc atttattcag ataattgctg actgactcga taatttaatt
     2941 tttaataaat ttcaaattca aacattaaag taagtacatt cttttgaaat attttgcatg
     3001 agtagaaaag aaaaattcaa ccttcaatag gggaaatact attgaaaatc aactaaccac
     3061 gaacaaaact cagcccctca gctaacgacg agcgacttgg gttgcggaac gactgacgag
     3121 gtagttttca agcttgaac
//
LOCUS       DAOTFA010000051         3112 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate AMR_MDS_3115
            NODE_24320_length_3112_cov_8.572457, whole genome shotgun sequence.
ACCESSION   DAOTFA010000051 DAOTFA010000000
VERSION     DAOTFA010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868139
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (wastewater metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:36:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 536
            CDSs (total)                      :: 521
            Genes (coding)                    :: 520
            CDSs (with protein)               :: 520
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_24320_length_3112_cov_8.572457"
                     /isolate="AMR_MDS_3115"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="P5014_02135"
     CDS             complement(<1..437)
                     /locus_tag="P5014_02135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56942.1"
                     /translation="MFKPISRKNSGEKTAFLILSILLAGVIVIKLVYAATPNPGHDFT
                     QVSGSVAQGDLLFGSAEDTLSKLAKDANATRYLSNTGTDNNPAWAQVELSNGVTGNLP
                     VANMDSGSNASSSTFWRGDGTWQPTTSKAVVGGGSSTSITADAV"
     gene            637..2415
                     /gene="dnaG"
                     /locus_tag="P5014_02140"
     CDS             637..2415
                     /gene="dnaG"
                     /locus_tag="P5014_02140"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HRY56943.1"
                     /translation="MEDIVTQIKQKLDIVDVINSYVSVKKSGRNYKAVCPFHNEKTPS
                     FMVSQELQIYKCFGCGAAGDMFNFVQAIEGVDFPRSLEILADRSGVKIPKSENFDNQN
                     QIKKKIFQINEITTKFYQYVLLNQKAGKKALDYLVKERKIIEKSIKDFRLGYAPDSWD
                     TLVKFLRSKGFKDEDLILSGVAVKRNVGEGLIDKFRGRVVFPFVAVDEKILGFTGRTL
                     FDRDPKYLNTGETPVFHKSFFLFGLNKSRVNIKKEGAIFVEGQTDVISAHQNGVDNVI
                     CVSGTSLTDNQLDILSRYTQDITFCFDSDFAGIEASYRAIEMAGNKNFNVNIAVIPEP
                     YKDLDELLRDDPAKAKKILKTPIPVYDFFLATALKRNNKETALGKKAIMEELVPVFSR
                     ISNRVVFEHYAKQLSSELDISENTVFSMLEQKKSSKNTEYPEFEDFGQKFPLKDRKIE
                     GYLISLLLKAPLEKSKEIVKKLKKEDFLNENLADVFEKLENYLEDRKSEINVKAFINK
                     FEDEQKNLISELYMWDSDEDEESNEFPKNIEKELEEVSERVKKDSIRKQLKNLAESIK
                     IAEIEKEEDELERLTKKFEKLSKSLL"
     gene            2493..>3112
                     /locus_tag="P5014_02145"
     CDS             2493..>3112
                     /locus_tag="P5014_02145"
                     /inference="COORDINATES: protein motif:HMM:NF015912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor region1.1
                     domain-containing protein"
                     /protein_id="HRY56944.1"
                     /translation="MPKIIKKEVSKKKVPSRGNGKKIVADKKKISVKKVVKTQKDSSK
                     KSSIKVPIKKVLKTKVSLSKKPIQKKEEAKVVVKKEKELKVPAKKIEKEGNKEKNIQN
                     IEAKESRLKINSQELGIKKLIKKGKKQGYLNLDDLLTLFPNAEEDIETLDFLYEKLME
                     SGIDVFDIGSEKEAEKVAKLEEIDLSKIKLDKTTTSDPVRMYLKEIG"
BASE COUNT         1147 a          454 c          602 g          909 t
ORIGIN      
        1 accgcatctg cggtaattga agtagaactt ccccctccaa ctacagcttt tgatgtcgta
       61 ggttgccatg ttccgtcccc tctccaaaaa gttgaagagc tggcatttga cccagaatcc
      121 atatttgcta caggtaaatt tcctgtcacc ccatttgaaa gttctacttg cgcccacgcg
      181 gggttgttat ccgttcccgt gtttgataaa tatcttgtag cattggcgtc ttttgcaagt
      241 tttgataatg tgtcttcagc agatccgaaa agaaggtccc cctgagctac acttccgctt
      301 acttgagtaa aatcgtgtcc gggattaggg gttgctgcat aaacaagctt aattactatt
      361 actccggcaa gcagtataga tagtattaag aacgctgtct tttctccgga atttttccta
      421 cttattggtt taaacatact atatatagaa tatattaagt gctttaaaat ttccacccaa
      481 ttttattctg atagttcaga gtccaaaaaa acatacattt ggtacttaag atttagacca
      541 ataaggcttt aaaacctgat acattcatga tatatagttc ttaccaaagg agattcacgc
      601 actttctgcc tttttgcggt aatattaaat aaaaaaatgg aagatatcgt tactcaaata
      661 aaacaaaagt tagatattgt ggatgtaata aatagttatg taagtgtgaa aaaatcggga
      721 agaaattata aagccgtttg tccttttcat aatgaaaaaa caccctcttt tatggtttcc
      781 caggaattgc agatatataa atgtttcggg tgcggggcgg cgggggatat gtttaatttt
      841 gttcaggcta tagagggcgt tgattttcca agatcactgg aaatacttgc tgacagatca
      901 ggggtaaaaa ttcctaaatc cgaaaatttt gataatcaga atcagattaa gaaaaaaata
      961 tttcagatta atgaaattac aacaaaattt tatcaatacg tacttcttaa tcagaaagcc
     1021 ggaaaaaagg ctttggatta tttagtaaaa gaaagaaaga taattgaaaa atccataaaa
     1081 gattttagat taggttatgc tcctgattct tgggatacac ttgttaagtt tttaaggtcc
     1141 aagggtttta aagacgaaga tttaatatta tcaggagttg ccgtaaagag aaatgtcgga
     1201 gaaggtctta tagataaatt tagagggaga gtggtatttc catttgttgc agtagatgaa
     1261 aagatacttg gttttaccgg aagaacactt tttgacaggg accctaaata tttaaataca
     1321 ggcgagaccc ccgtttttca taaatccttc tttttatttg gtttaaataa aagcagagta
     1381 aatattaaaa aagaaggtgc aatctttgtg gaaggccaaa ccgatgttat tagtgcccat
     1441 caaaacggag tagataatgt catatgtgtt tcgggcacat ctctcacaga caaccagttg
     1501 gatattcttt cgaggtatac ccaggatatt acgttttgct ttgattctga ttttgccgga
     1561 atagaggctt cttatagagc tattgaaatg gcaggaaata aaaattttaa tgtaaatatt
     1621 gctgttatac ccgagcctta taaagatctc gatgagcttt taagagacga tcccgctaaa
     1681 gctaaaaaaa tattaaaaac cccgatccct gtgtatgatt ttttccttgc cactgctcta
     1741 aaaagaaata ataaggaaac tgctctgggc aagaaggcca tcatggagga gttggtgccg
     1801 gttttttcca gaatatccaa tcgggttgtg tttgaacatt atgcaaaaca actttcatcg
     1861 gagcttgata tatcagaaaa tacggtcttt agtatgttag agcaaaaaaa gtcttcgaaa
     1921 aacactgaat accctgagtt cgaagatttc ggacaaaaat ttcctttaaa ggataggaaa
     1981 atagaggggt accttatatc cttgcttttg aaagctcctc ttgaaaaaag caaagaaatt
     2041 gtgaaaaaac ttaaaaaaga ggatttcctt aacgagaatt tagctgacgt ttttgaaaaa
     2101 ttggaaaatt atctcgagga caggaaatca gaaattaacg taaaagcatt tataaataaa
     2161 tttgaagatg agcagaagaa cttaatctcc gaactttata tgtgggattc tgacgaagat
     2221 gaggaaagca atgaatttcc taaaaatatc gaaaaagagc tggaagaagt gtcggaaaga
     2281 gtgaaaaagg attctataag aaagcagttg aaaaatttgg cggaaagtat taaaatagcc
     2341 gagattgaaa aggaggagga cgaattagag aggcttacta aaaagttcga aaaattgtca
     2401 aaaagccttt tatagagtat aaaattccta tattaaattc atttataata ttctctgtgt
     2461 ccttcggggc gttgtttcta taataaaaaa atatgccaaa aataataaaa aaagaagttt
     2521 ctaaaaagaa agttccttct aggggaaacg gtaagaaaat agttgcagat aagaagaaaa
     2581 tatctgttaa aaaggtagtt aaaacacaaa aagattcctc aaaaaaatct tctataaaag
     2641 taccaattaa aaaggtactc aaaacgaagg ttagtttatc taaaaaacct attcagaaaa
     2701 aggaagaggc taaggtagtt gttaaaaagg aaaaggaatt aaaagtacct gctaaaaaaa
     2761 ttgaaaaaga gggaaataaa gaaaaaaata tacaaaacat tgaggctaaa gaaagcaggt
     2821 taaaaataaa ttctcaggaa ttgggtatta aaaaactaat taaaaagggt aaaaaacaag
     2881 ggtacttaaa tcttgatgat ttactaactt tatttccgaa cgcagaggag gatattgaaa
     2941 ccttggattt cctttatgag aaattgatgg aatccggcat agacgtcttt gatattggtt
     3001 ctgaaaaaga ggctgaaaaa gtcgcaaaac tcgaagagat agatttatcc aagataaaat
     3061 tagataaaac tactacatcc gatcctgtaa gaatgtattt aaaagaaatt gg
//
LOCUS       DAOTFA010000052         2873 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate AMR_MDS_3115
            NODE_27385_length_2873_cov_7.467353, whole genome shotgun sequence.
ACCESSION   DAOTFA010000052 DAOTFA010000000
VERSION     DAOTFA010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868139
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (wastewater metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2873)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2873)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:36:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 536
            CDSs (total)                      :: 521
            Genes (coding)                    :: 520
            CDSs (with protein)               :: 520
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2873
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27385_length_2873_cov_7.467353"
                     /isolate="AMR_MDS_3115"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="P5014_02150"
     CDS             <1..303
                     /locus_tag="P5014_02150"
                     /inference="COORDINATES: protein motif:HMM:NF012970.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56945.1"
                     /translation="VYATSISEIKKIVGTKDFYFTDVERGINLLVRNTNRLLWEIPNS
                     VGVKTGTTFEAGEVLIYEYDDGIKDILVVVMGSKNRFADTKNILSWIGRNYSWKIL"
     gene            complement(380..1501)
                     /locus_tag="P5014_02155"
     CDS             complement(380..1501)
                     /locus_tag="P5014_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56946.1"
                     /translation="MNLTKTAELVKKVIKYLFLIITSYYLVAYVILPGSVSLIKAMFT
                     KRIPPNTIYNQLDQLEFTKKKINNENITYSINTTNGKLPSGFPDKMKVYKFKPAQYSY
                     LAGKNASAEAAVLGFGDEDLRSDLTVTVYRWRNSRTMSNLSIDINTRQINLETDMEGR
                     SSNFDKGSVNKDSAVQMGKDTMSSIFRFNDGLYPNGKQNVKLGYYIGNKLFETENQNE
                     AQVALVDFYRSIEEYPILGPDPSKGLMRMIVGAKFKQPNPLSNPYLEAYYWEIDTQTE
                     AMYPIITVQEAWKMVTGGKAVITQVTPRQSNPFEPYYPVSVDKVLIDNIYLAYYETPK
                     FQTYLQPIYVFSGKYTTRGTEGGDITLYFPAITGEWTKQ"
     gene            1586..>2873
                     /gene="gltX"
                     /locus_tag="P5014_02160"
     CDS             1586..>2873
                     /gene="gltX"
                     /locus_tag="P5014_02160"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HRY56947.1"
                     /translation="MKMRTRLAPSPTGEIHVGSIRTLLYDYALAKQSEGELVLRIEDT
                     DQSRYVEGSVERMLKVIKDYGLKWDEGPEVGGPYQPYFQSQRLDIYKKYALELVEKGN
                     AYYCFCDEDRLNKVREGQKEMGGVTKYDRFCLNLSKEDVEENLKKGKPYVIRMKVPSD
                     VDIEVEDMVLGKLTFPSNDLDDQVLIKSDGFPTYHLAVVVDDHLMKITHVLRGREWLP
                     STPKHVLLYEFFGWEKPKFIHLPLLRETDSTKKLSKRTGSVSALGFLEEGYLPEALLN
                     FMMFLGWNPGTEREVYSLDDFVKDFSIEKIQKSEMAAFDRQKLLWFNGLYIRNLPLED
                     LWNRVVKWHEEYSEKLNLKNSTDARNLKILEIVRERMKKLSEFNDITFYFFEEPKIDK
                     KTAEKFSGDSKKAHEIIDNFIEVYKNIPSEKWNVPDL"
BASE COUNT          932 a          446 c          564 g          931 t
ORIGIN      
        1 gtatatgcaa cttctatttc ggagattaaa aaaattgtgg gtacaaaaga tttttatttt
       61 acagatgttg agaggggtat aaatttgtta gttagaaata ccaacagact tttatgggaa
      121 atacctaata gtgtgggtgt aaagacgggg accacgtttg aagctgggga ggttttgata
      181 tatgaatatg atgacggaat taaagacata ttagttgtag taatgggtag taaaaataga
      241 tttgcagata caaaaaatat tcttagttgg ataggtagaa attactcttg gaagattctt
      301 tgaaaaactc tttttgaaaa attactcacg gtatccaaaa actcattcca gttttttaaa
      361 atttaaagtt actaaattat tattgcttag tccactctcc cgttatagca gggaagtata
      421 aagttatatc tcctccttca gttcctctcg tggtgtattt tcctgaaaag acatagatag
      481 gttgaaggta tgtttgaaac tttggagttt cgtaataggc taagtagata ttatcaatta
      541 atactttatc tacacttacg gggtagtaag gttcaaaagg atttgattgt ctcggtgtta
      601 cttgagtaat gactgccttt cctccggtaa ccattttcca agcttcttgc actgtaatta
      661 ttgggtacat tgcttctgtt tgtgtatcta tttcccagta atacgcttcc aaataaggat
      721 tgcttaaagg atttggctgc ttaaattttg cacctacaat cattctcatt aagccttttg
      781 atgggtccgg tccaagtatt gggtattctt ctattgatct ataaaaatca acgagtgcaa
      841 cttgagcttc gttttggttt tctgtttcaa ataatttatt tcctatatag tagccaagtt
      901 tgacgttctg tttgccattt ggatacaaac catcgttaaa tctaaatatg gaagacatcg
      961 tatctttacc catttggaca gcactgtctt tgttaacgga tcctttatca aaattagagc
     1021 ttcttccttc catatcagtt tctaaattaa tctgtctggt gttaatatca atcgataaat
     1081 tactcatagt ccttgagttt ctccatctat atactgtcac cgtaagatcc gatctaagat
     1141 cctcatcccc gaaccctagc accgcagcct cggcggatgc gtttttacct gctaaatagg
     1201 aatattgtgc cggtttgaat ttataaactt tcatcttgtc gggaaatccc gaaggtagtt
     1261 ttccgttagt ggtattaatc gagtaagtaa tattttcgtt attaattttc tttttagtaa
     1321 attctaattg atctaattgg ttatatatag tgtttggcgg tattcttttg gtaaacattg
     1381 ccttaattaa tgagactgac ccgggtagta tgacataagc tacaaggtaa taggaggtta
     1441 ttattaaaaa caaatatttg ataacttttt taactagttc ggcagtttta gtaagattca
     1501 tttgtttaat tttaacagat acctaagtca attccgaggt acctctttat attacaccat
     1561 ttgttttcgt gctaaaatcg aaaatatgaa gatgagaacc agattagcac caagtccgac
     1621 cggtgagatt cacgtcggaa gtataagaac acttttgtat gactatgctt tagcaaaaca
     1681 gtctgaaggt gagttagttt taagaataga ggacactgat caaagcagat atgttgaggg
     1741 ttcggttgaa aggatgctta aagtcatcaa agattacggc ttgaaatggg acgaaggccc
     1801 ggaggtcggt ggtccttacc aaccttattt tcagtcacaa agattagata tttacaaaaa
     1861 atacgccttg gaattagtag aaaaaggtaa cgcctactat tgtttttgcg atgaggatag
     1921 attaaacaaa gtaagagagg ggcagaaaga gatgggcggg gttacaaaat acgatagatt
     1981 ttgtcttaac ttaagcaagg aagatgtcga agagaattta aaaaaaggaa aaccctatgt
     2041 catcagaatg aaagttccat cagatgtaga tattgaggtt gaagatatgg ttttagggaa
     2101 actcacattt ccttccaacg acttggatga tcaggtttta ataaaatcgg atggtttccc
     2161 aacataccat ttagctgtcg tagttgatga tcacctaatg aaaatcacac atgttttaag
     2221 aggtagagag tggctaccct ctacaccaaa acacgtactt ttatacgaat tctttggctg
     2281 ggagaaacca aaatttatac atcttccttt attgagggaa accgacagca ccaagaaatt
     2341 atcaaaaaga acgggatcgg tgagtgccct tgggttttta gaagaagggt accttccgga
     2401 agcgctttta aactttatga tgtttttagg atggaacccc ggcaccgagc gggaagtgta
     2461 ttcactggat gattttgtaa aagacttttc aatagaaaaa atacaaaaat cagagatggc
     2521 cgcttttgat agacaaaaac ttttatggtt caacggactt tatattagaa atttaccatt
     2581 agaagattta tggaacagag tagtaaagtg gcacgaagag tattcagaaa aactcaattt
     2641 gaaaaattct actgacgcta gaaatttaaa aatattagaa attgtccgag agagaatgaa
     2701 aaagcttagt gagtttaacg acattacttt ttacttcttt gaagaaccca aaatagataa
     2761 aaaaactgcg gaaaaatttt caggtgattc aaaaaaagct cacgaaataa ttgataactt
     2821 cattgaagtt tataaaaata tcccgagcga aaaatggaac gttcctgacc ttg
//
LOCUS       DAOTFA010000053         2850 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate AMR_MDS_3115
            NODE_27782_length_2850_cov_6.188551, whole genome shotgun sequence.
ACCESSION   DAOTFA010000053 DAOTFA010000000
VERSION     DAOTFA010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868139
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (wastewater metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2850)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2850)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:36:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 536
            CDSs (total)                      :: 521
            Genes (coding)                    :: 520
            CDSs (with protein)               :: 520
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2850
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_27782_length_2850_cov_6.188551"
                     /isolate="AMR_MDS_3115"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..875
                     /locus_tag="P5014_02165"
     CDS             <1..875
                     /locus_tag="P5014_02165"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRY56948.1"
                     /translation="SIMKPMTVSAAVDLGLVGPDTTFVDSGPVVYSTHTIDNWDGKHY
                     GVQTIAQLLQKSNNIGAAWVGMQVGRDNLYKYLSDFGIGTTSGIDLEGEDSGILRPKD
                     SWTDIDTANISFGQGVSTTPLQMLSAFNVIANGGFLMQPKIISKLVDENGETEIPSKN
                     IRRVISKETSDIMTDLLEKAAEGGEGKYFILKDYRISGKTGTAEVPEEGSYSKNRTNA
                     TFVGFLTQSKAFSLIVKLQEPQESPWAAETAVPVWMDIASELVKFYGIAPDRVVENPV
                     TSTIKSETNPSLNQ"
     gene            964..1326
                     /locus_tag="P5014_02170"
     CDS             964..1326
                     /locus_tag="P5014_02170"
                     /inference="COORDINATES: protein motif:HMM:NF014119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HRY56949.1"
                     /translation="MNKRCVGKVAERIAVTYLKSRGYKIITTNWTCLGGELDIVAYKR
                     KLTFVEVKSAESGFCSPCDLFNYKKKCHLSRTINSYLVKNYEYSVKIPDYSVDLISIE
                     KNKGQYKIKHYFDIDLSI"
     gene            1413..1817
                     /locus_tag="P5014_02175"
     CDS             1413..1817
                     /locus_tag="P5014_02175"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRY56950.1"
                     /translation="MPKKIFFVEDDEGFYNIFSVPLKMKGYDIVRVSEGTSAVERIIT
                     EKPDLILLDIVLPGMSGLDILKELREREETQSVVVIMLTNFGSDENISRAMEYGANDY
                     LMKYNIVPSELPDKIALFLGEDQDFNVKVMGS"
     gene            1827..2660
                     /locus_tag="P5014_02180"
     CDS             1827..2660
                     /locus_tag="P5014_02180"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HRY56951.1"
                     /translation="MEKITILNILVILESIILLIISFILFFRLKDLSKLKKLQDELND
                     LKSSKNSARDDFLPILVHELRSPLSVIRGAADLLIKSTDELSVVQIQTLLNQIKNSST
                     TLLTMVGSILDVSKMENGKFEINKTFSDINSVLNEECSYFDPLTKIKKLSIQCTVDKD
                     IPNFSFDIERVKQVLNNLISNAIKFSPEGAVITVTSKKTGSDCVVYVSDLGVGIPEHE
                     KNSLFQKFFQASNQNGVQKGTGLGLFISKGIVEAHGGKIWFENNSPQGSKFCFSIPIK
                     S"
     gene            2714..>2850
                     /locus_tag="P5014_02185"
     CDS             2714..>2850
                     /locus_tag="P5014_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014767668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HRY56952.1"
                     /translation="MTKKTAHVLWFKDINKNDIPLVGGKGANLGEMVKAGIPVPDGFV
                     VT"
BASE COUNT          969 a          435 c          544 g          902 t
ORIGIN      
        1 gatctatcat gaaacctatg accgtatctg cagcagtaga tcttggttta gttggtcctg
       61 atactacatt tgtcgatagt ggtccggtgg tttattctac ccatacaatt gataactggg
      121 atggaaaaca ttacggtgtc cagaccatag cccaattact tcagaaatca aacaatattg
      181 gagcagcttg ggttggtatg caggttgggc gtgataactt atataaatat ctttctgatt
      241 ttggcattgg aactacatcg ggtattgatc tggaaggtga ggatagcgga attttaaggc
      301 caaaagatag ctggactgat atcgatacgg ccaatatatc ctttggacaa ggtgtttcca
      361 ctacacctct tcaaatgctt tcagctttta acgtcatagc aaatggaggt tttctaatgc
      421 agccaaaaat aatatcaaaa ctagttgatg agaatggaga aaccgaaata ccctctaaaa
      481 atattagaag agttatatct aaggagactt cggatattat gacagatctt cttgaaaagg
      541 ctgcagaagg tggtgagggt aaatacttta ttttaaaaga ttatcgaata tcaggaaaaa
      601 caggtacagc ggaggttccg gaagaaggaa gttattcaaa aaatagaact aatgctacat
      661 ttgttggatt tttaactcaa tccaaagcat tttcattgat tgtaaaactg caggaacctc
      721 aagaatcgcc ttgggcagca gaaacagcgg ttccagtttg gatggacatt gcatctgagc
      781 ttgttaagtt ttacggtatt gctccggaca gggttgttga aaaccctgtc acctcaacta
      841 taaaatcgga gacaaatcct tcactaaacc aataaatctc aatattttat taaaaatgaa
      901 aaattctatt aatagtagaa tcagatacgt tgataaaaat tcattaagat ttatatttat
      961 ctcgtgaata agaggtgtgt ggggaaggta gcggaacgaa tcgctgttac ttacctgaag
     1021 agcaggggct acaaaattat tactaccaat tggacgtgtt tgggcgggga gttagatatt
     1081 gtggcctaca aacgtaaact tacatttgta gaggttaaat ccgctgaaag cggattttgt
     1141 agcccctgcg acctgtttaa ttataaaaag aaatgtcacc tatcaagaac cattaattca
     1201 tatcttgtta aaaattacga atatagtgta aaaattccgg attattctgt tgatcttata
     1261 agtatagaaa aaaataaagg ccagtataaa ataaaacatt attttgatat agatttgtct
     1321 atttagagat aaatagtgca gaggtaagat gattttctac ttctttcata aaaacggttt
     1381 acttgtttga attccttttg taatattact gtatgcctaa gaaaatattt tttgttgagg
     1441 acgatgaggg tttttataat atattctccg ttcccctaaa gatgaaagga tatgatattg
     1501 tcagggtttc cgaaggaacc tctgcggtag aaagaattat caccgaaaaa ccagatctta
     1561 ttcttttaga tatagtttta cctggtatga gcggtttgga cattctaaaa gaacttcgtg
     1621 agagagaaga gacccaatca gttgttgtta taatgcttac aaacttcggt agtgatgaaa
     1681 acatctcgag agctatggaa tacggagcaa atgattactt aatgaaatat aacatcgttc
     1741 cttctgaact gcccgacaag atagctttat ttttaggtga agatcaagac tttaatgtaa
     1801 aggttatggg atcgtaaact gccacaatgg aaaaaataac aatactaaat attctggtaa
     1861 tactggaatc aataattctt ttaattattt catttatctt attttttagg ttaaaggact
     1921 tatctaaatt aaaaaaactt caagatgaac tcaacgacct aaaatcttct aaaaattcag
     1981 ctagagatga ttttttacct attcttgttc atgaattaag atcacctctg tcagtaataa
     2041 gaggtgccgc agatttgtta attaaaagta cagacgaact gtcggtagtt caaatacaaa
     2101 ctcttctgaa tcagattaaa aactcttcta caactcttct cactatggtg ggaagtattt
     2161 tggatgtctc aaaaatggaa aacggtaaat ttgaaattaa caaaacattc agcgatatta
     2221 attctgtttt aaatgaagag tgttcatatt ttgaccctct tacaaaaata aaaaaattaa
     2281 gtattcaatg tacagttgat aaagatattc ctaattttag tttcgatatt gagagagtga
     2341 agcaggtttt aaacaatctg atatcaaatg ccattaagtt tagtcccgaa ggtgcagtaa
     2401 ttacagtaac atcaaaaaaa acgggctccg attgtgtagt ttatgtttct gaccttggag
     2461 ttggcatacc agaacatgag aaaaattctc ttttccaaaa attctttcaa gcgtctaatc
     2521 aaaacggcgt tcaaaaagga actggtttag gactttttat ctcaaaggga attgttgaag
     2581 ctcacggagg taagatttgg tttgaaaata attctcctca aggatcaaaa ttttgtttct
     2641 ctatccctat taaatcttag atttaaagta aaaagctatt gcccgtaaaa tttttcaatg
     2701 ttaacatttt cacatgacta aaaaaactgc tcatgtcctt tggtttaagg acattaataa
     2761 aaatgatatt cctttggtag gtggtaaggg tgcaaattta ggagagatgg taaaagccgg
     2821 tattcccgtc cccgatggtt ttgtggtaac
//
LOCUS       DAOTFA010000054         2835 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate AMR_MDS_3115
            NODE_28007_length_2835_cov_8.348921, whole genome shotgun sequence.
ACCESSION   DAOTFA010000054 DAOTFA010000000
VERSION     DAOTFA010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868139
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (wastewater metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2835)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2835)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:36:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 536
            CDSs (total)                      :: 521
            Genes (coding)                    :: 520
            CDSs (with protein)               :: 520
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2835
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28007_length_2835_cov_8.348921"
                     /isolate="AMR_MDS_3115"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(321..668)
                     /gene="rpsT"
                     /locus_tag="P5014_02190"
     CDS             complement(321..668)
                     /gene="rpsT"
                     /locus_tag="P5014_02190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HRY56953.1"
                     /translation="MILYNPDMANTKSAKKNIRSSANKKSHNDMWEHRIKTAKKSLSR
                     AILSKEDKKLVSDKMIVLQKSLDKAVKEKTIHKNKANRVKSIYARKVAALDLKKEDKV
                     ESKPRKSTTKKSK"
     gene            707..2371
                     /gene="murJ"
                     /locus_tag="P5014_02195"
     CDS             707..2371
                     /gene="murJ"
                     /locus_tag="P5014_02195"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HRY56954.1"
                     /translation="MLLNFKNKNLNGFLFKTQNTILSAAVILGVASGVSAVLGLVKTR
                     LLASYFGVSKELTIFYTADKIPNLIYSILIVGAVSTVFIPIFTGLLKKDKEEAFKVAS
                     SIIGTTFIFFITVGVIMFAFSPNIIKLLSVGKFSDEDIKLGSNLMRIMLASQIFLVAG
                     SLSTSVLQSFKYFIIPAIAPIFYNIGMIAGTVFLSSKLGIYGPAIGVAMGSIMHFIIQ
                     IPLLKKSGFRLFSRIDLADKNFKKTFSLVPPRIASVLIVNLVQTINNSLAILVSAPSV
                     IFLKFADQLQTLPVNLFGISMGAASLPTLSSQSAPEEREKFKETFLTSLHQTIYLVMP
                     LSAILLVLRVPAIRLVYGVGNFPWEATIRTAQTLAFFSISIFSQSAGYLTTRAFYALK
                     DTSTPVKVNILTAIINIFVSVYFVSTLKLGVWSIALSYAGTSMLDLLLLIYFLNKKTG
                     GLDKKKLIVPFTKISFATGLMGIMLYIPMKLLDKYVLDTTRTINLIALTGLAGIVGMA
                     TYLLITHLLKVEEIQLFYKLLRKLRLSKGDIKAEAVDLATTEQETV"
     gene            2379..>2835
                     /locus_tag="P5014_02200"
     CDS             2379..>2835
                     /locus_tag="P5014_02200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015423885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HRY56955.1"
                     /translation="MFQEKIRNFCIVAHIDHGKSTLADRLLEITHTLDKRNIIEQTLD
                     SMDLEREKGITIKLKSIRMEYQGYQLNLIDTPGHVDFSYEVSRSLEACEGAILLVDKT
                     QGIQAQTVSNFYKATDSNLKIIPVINKIDLDTGDIEKIKKDMCQTFGFED"
BASE COUNT          918 a          488 c          467 g          962 t
ORIGIN      
        1 tgtccgatat taaaaaccta cttgataaaa actttttata cgtaatggtc ttatcactaa
       61 ttctctttgc tttttcttca atatctgaat aagattctat ttcctcaacc aatttactct
      121 gccacccttt ttgatattga atcatgaagg aaagggtaaa attaagtatt aatagaacac
      181 caaaaagtac acttatgcca acgatgaatt ttcttattat gagtataaat tcagatgcaa
      241 acttaggggt aatatataat ttctgagatt tagaaattag atcgaaattg gaatctaagg
      301 aaacactctc cttattgtcg ttacttagat ttctttgttg ttgattttct tggtttagat
      361 tcgaccttat cttctttttt aagatcgagg gcagcaactt ttctagcata tatagatttt
      421 accctatttg ccttgttttt atgaatagtt ttttctttga cagctttatc aagtgacttc
      481 tgtaaaacaa tcattttatc tgaaaccaat ttcttatcct cttttgataa gatagctctg
      541 gataaggatt tcttcgctgt tttaattcta tgctcccaca tatcgttatg ggacttttta
      601 ttagccgaag atcttatatt cttttttgct gacttagtat ttgccatatc agggttgtat
      661 aatatcataa ctgtaggaaa gtaaaaagca catttcctgt aaatgaatgt tattaaattt
      721 taaaaacaaa aacttaaacg ggtttttatt taaaacccaa aatacaattc tttcggctgc
      781 tgtaatatta ggcgtagcaa gtggtgtgag cgccgttctg ggtcttgtaa aaacaaggtt
      841 gttggcaagt tactttgggg tttcaaaaga attaactatc ttttacactg cggataaaat
      901 accgaatctt atatattcaa tcttaattgt gggtgccgta tcaacggtat ttattcccat
      961 attcacaggc cttttaaaaa aagataaaga agaagccttt aaggtagctt cgtcaattat
     1021 cggaactact tttatatttt tcatcactgt tggggtaatt atgtttgcct tctcaccaaa
     1081 tataattaag cttctatctg tcggtaaatt ttcagacgaa gacatcaaac tcggttcaaa
     1141 tcttatgcgg ataatgctgg cttcacagat atttctggtt gcaggaagcc tgtctacaag
     1201 cgttctgcaa tcatttaaat attttataat cccagctata gctcccattt tttacaacat
     1261 tggaatgatc gccggaacag tttttttatc cagcaaattg gggatttacg gacctgcaat
     1321 aggggtagca atgggctcaa taatgcattt tataatccaa atcccgttgt taaaaaaatc
     1381 aggatttaga ttgttttctc gtatagatct agcggacaaa aacttcaaaa aaacgttctc
     1441 gctcgtacct ccaagaatag ccagtgtttt aatagttaat ttagtacaaa caataaacaa
     1501 cagtttagca atattagttt cagcaccttc cgtaatattt ttaaaatttg ccgatcagct
     1561 tcaaacactt cctgtaaatt tatttggaat atctatgggt gcggccagct tacctactct
     1621 atcctctcaa tccgctcccg aagaaagaga gaaatttaaa gaaacatttt taacttcttt
     1681 gcaccaaaca atttatttgg taatgccgtt gagtgctatc cttttagtac ttagagttcc
     1741 tgcaataaga ctggtttacg gggtaggaaa ttttccatgg gaagcaacta taagaactgc
     1801 acagacacta gctttcttta gtatttcaat attttcccag agtgccggct atttaactac
     1861 cagagctttt tatgcactta aggacacctc aaccccggtt aaagtaaaca ttttaacagc
     1921 aattattaac atttttgtat ctgtttactt tgttagcaca ctgaagctcg gggtgtggtc
     1981 tattgcttta tcttatgcgg ggacatctat gctagatctc ctccttctaa tatattttct
     2041 taataaaaaa acaggtggtc tggataagaa aaaactcatt gtcccattta ctaaaatttc
     2101 ttttgcaaca ggtttaatgg gaataatgct ttatatacca atgaaacttt tggataaata
     2161 cgtgttggac actaccagaa caattaatct tatagctctt acaggattgg ctggaatcgt
     2221 gggcatggca acgtatcttt taattacaca cttattaaaa gttgaggaaa ttcagctttt
     2281 ttataaatta ctaagaaaat taagattatc aaaaggcgat attaaggcag aggccgtaga
     2341 tttagcgaca actgaacaag aaactgtata atcaacctat gtttcaagag aaaatccgaa
     2401 atttttgcat agtagcccac atagaccatg gtaaatctac cttggcagac aggctattag
     2461 aaattaccca tactttagac aaaagaaaca taattgaaca aactttggac tcaatggatt
     2521 tggaaagaga aaaaggaatt accattaaac ttaaatccat aagaatggaa taccaagggt
     2581 accaattaaa tcttatcgat accccgggcc atgttgattt ttcatacgag gtttcaagat
     2641 cactggaagc ctgtgagggc gccattcttt tagtcgataa aacccaaggg attcaagctc
     2701 aaactgtttc taatttttat aaagcaacag actcgaattt aaaaattatt ccggtcatta
     2761 ataaaataga tttagataca ggagatattg aaaaaattaa aaaagatatg tgccaaacat
     2821 tcggttttga agata
//
LOCUS       DAOTFA010000055         2765 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate AMR_MDS_3115
            NODE_29074_length_2765_cov_5.072325, whole genome shotgun sequence.
ACCESSION   DAOTFA010000055 DAOTFA010000000
VERSION     DAOTFA010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868139
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (wastewater metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2765)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2765)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:36:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 536
            CDSs (total)                      :: 521
            Genes (coding)                    :: 520
            CDSs (with protein)               :: 520
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2765
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29074_length_2765_cov_5.072325"
                     /isolate="AMR_MDS_3115"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..468)
                     /locus_tag="P5014_02205"
     CDS             complement(<1..468)
                     /locus_tag="P5014_02205"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HRY56956.1"
                     /translation="MFDYSKQKDENQNISKYWRKNPKSIEKYQFVHHFLPKPDKRKRA
                     DFLENKAILIYCVFILVITAVFRIIPKVAPGVLGYASDINITDLLKYTNKKRGENGVS
                     NLRLNDKLSAAAQKKAKDMFEDGYWAHVAPDGTEPWDFIVGEDYEYIYAGENLA"
     gene            complement(493..1110)
                     /locus_tag="P5014_02210"
     CDS             complement(493..1110)
                     /locus_tag="P5014_02210"
                     /EC_number="2.7.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050145 - nucleoside monophosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside monophosphate kinase"
                     /protein_id="HRY56957.1"
                     /translation="MKILLIGSQGCGKGTVGSMLSDYLSIPLISAGHILREVSENHPR
                     KKEIQDYMARGELVPQDLVADLLREETSKDSSKNGFIFDGWGRTLMDLNFYDPDFDKV
                     IFISISPETSVKRISSRRTCDNCGSVFNVVSVPPKVEGICDECGGELIQREDDTEEAV
                     RRRLQIFNTETSDVIEHFRNEGKLVEIDGEGSPEEVFKLALEALK"
     gene            complement(1128..1394)
                     /locus_tag="P5014_02215"
     CDS             complement(1128..1394)
                     /locus_tag="P5014_02215"
                     /inference="COORDINATES: protein motif:HMM:NF014616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HRY56958.1"
                     /translation="MFDKIKELNKFKQAQSKIKKQMEDISATREKDGIKIVVRGDKKI
                     QKIEVDGEDQKELKDLINDTFSDVDKKLQKQMRGQLQDLGLPDF"
     gene            complement(1422..2678)
                     /gene="secY"
                     /locus_tag="P5014_02220"
     CDS             complement(1422..2678)
                     /gene="secY"
                     /locus_tag="P5014_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020256960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HRY56959.1"
                     /translation="MLKIFKLPDLRKKIIFTLFIIAIFRLLAHIPVPGVDTSSIKAYL
                     EKSVIFGFFDLFSGGGFQNFSIVTLGLGPYINASIIIQLLTMLIPSLEEMAKEGEYGQ
                     EKINMYTKVLAIPMAFVQAFGIYFLLSKQGIINSLGGFDVILLMLTFTAGSMLLVWLG
                     DLLTEKGVGNGISALIFVSIISRLPNSALSAVTGIMSSNYLQTVIVLLLILLTIIAVV
                     LVNEGTRNVLLEYGRSSERSRKVTNYLPIKINQAGVIPIIFAVSIVSIPGMVSGPFMA
                     SGKPVLVNIGAFMARYFEQKSLSYNALYFVLVVAFTFFYTFLQFNPEKIAEDVKKRGG
                     FIPGVRPGTSTAQYLKRIVSRITLMGSLFLGLIAILPYFMDFIFGPNVISIGGTSLLI
                     AVSVVLETVRQVESLTVTRSYEKFLG"
BASE COUNT          898 a          569 c          396 g          902 t
ORIGIN      
        1 tgccaaattc tctccggcgt aaatatattc gtaatcctca ccaacgataa agtcccaagg
       61 ttctgtacca tcaggggcta catgagccca gtaaccgtcc tcaaacatat ccttagcctt
      121 tttttgagct gcggcggata atttatcatt taatctaagg ttggatacac cgttctctcc
      181 tcttttttta ttagtatatt tcaataaatc tgtaatatta atatcagagg cgtaccctaa
      241 cacaccggga gcaacttttg gaattatcct gaaaactgca gtaataacta aaataaaaac
      301 acaataaatt aaaattgctt tattctctaa aaaatcagct ctttttctct tatcaggttt
      361 gggtaaaaaa tgatgaacaa actgatactt ctctatggac ttgggattct tcctccagta
      421 ttttgaaata ttctgatttt catctttctg ttttgagtag tcaaacatta actgtaatta
      481 tatcattata atttatttta gtgcttccaa agcaagctta aagacctctt ccggactacc
      541 ttcgccgtct atttcaacaa gttttccctc atttctaaaa tgctcaatta catccgaggt
      601 ctctgtgtta aatatctgta atcttcttct aactgcttct tcggtgtcat cttcacgctg
      661 gattagctca ccaccacact catcacaaat tccttcaacc tttggaggga cggacacaac
      721 attaaaaact gacccgcagt tatcacaggt tcttcgtgat gaaattcttt taacggaggt
      781 ttccggagag atactaatga aaattacttt atcgaaatcg ggatcgtaaa aattaagatc
      841 catcaaggtt ctgccccaac catcaaagat aaacccgttc ttactggaat ctttagaggt
      901 ttcttcccta agtaaatctg caactaaatc ctggggtacg agctcacccc tggccatata
      961 atcttgaatt tccttttttc tcggatgatt ttcggaaact tcccttaaaa tatgaccggc
     1021 agaaattaag gggatactta aataatcact taacatactt cctacggtgc ctttaccaca
     1081 cccttgagat cctattaata atattttcat ggtaataatt gtaaagctta aaaatcagga
     1141 agtccaagat cttgtagctg gcccctcatt tgtttttgta atttcttgtc aacatcgctg
     1201 aaagtatcgt ttattaaatc ttttaactct ttttgatcct ctccgtctac ctcaatcttc
     1261 tgaatttttt tatctcccct aactacaatt tttatcccat ctttttctct ggtagcagaa
     1321 atatcttcca tttgtttttt gattttactt tgagcttgtt taaatttatt tagttctttt
     1381 attttatcga acatattaat atcttatcag taaaaaataa attaccctaa aaacttctcg
     1441 taacttctcg taacggttaa agactctact tgccttaccg tctcaagtac cacagaaact
     1501 gcaatcaata gactggttcc cccaatggaa attacattag gaccaaatat aaagtccata
     1561 aaataaggca aaatggcaat taaaccaagg aataaagatc ccataagtgt aattctggaa
     1621 acaatccttt ttaaatattg tgcggtagag gttccgggcc ttacaccggg tataaaaccc
     1681 cctctcttct taacatcctc ggcaattttc tcaggattaa actgtaaaaa agtatagaaa
     1741 aatgtaaaag ctacaaccag tacgaaatat aaagcgttgt aagaaagaga tttttgttca
     1801 aaatatcttg ccataaaagc accgatatta actaaaacgg gttttccgga agccataaaa
     1861 gggccggata ccataccagg tatagaaaca attgacaccg cgaatattat tgggattacc
     1921 cccgcctggt ttatcttaat aggaaggtag ttggtaactt ttcttgatct ttcggaactt
     1981 ctaccgtact ccaaaagaac atttcttgtt ccttcattta ccaaaactac cgcaattatt
     2041 gttaataaaa ttaataataa tacaattaca gtttgaagat aatttgaaga cattattccc
     2101 gtaacggcag ataaagcact attaggcagc ctgctaatta ttgatacaaa aattaaagct
     2161 gatattccgt ttcccacccc tttttcagtt aacaaatcac caagccaaac taatagcata
     2221 gaaccagccg taaaagttaa cataagaaga attacatcaa aacctcccaa tgaatttatt
     2281 atgccctgtt tacttaataa aaagtaaata ccaaaggctt gaacgaaagc catcggaatt
     2341 gctaacacct ttgtatacat atttatcttc tcttgtccgt actcaccttc ttttgccatc
     2401 tcctccaaag aaggaataag catggtaagg agttggataa ttatagatgc gttgatataa
     2461 ggtcctaaac ctaaagtaac aattgaaaaa ttttgaaaac cacccccgct gaataaatca
     2521 aaaaaaccaa aaataacgct cttttcaagg taagctttaa ttgatgaggt atctactccc
     2581 ggcaccggta tgtgagccaa gagtctaaaa atagcaatta taaataaagt aaatattatt
     2641 ttcttcctta agtcaggaag cttaaatatt ttaagcattt attgtggcac cgaaactttc
     2701 gatcttttct ttagcttttt cggaatatgt aaaatcttta aaaactactt tctttttcaa
     2761 ctcac
//
LOCUS       DAOTFA010000056         2701 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate AMR_MDS_3115
            NODE_30168_length_2701_cov_4.622827, whole genome shotgun sequence.
ACCESSION   DAOTFA010000056 DAOTFA010000000
VERSION     DAOTFA010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868139
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (wastewater metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:36:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 536
            CDSs (total)                      :: 521
            Genes (coding)                    :: 520
            CDSs (with protein)               :: 520
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2701
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30168_length_2701_cov_4.622827"
                     /isolate="AMR_MDS_3115"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            101..1174
                     /locus_tag="P5014_02225"
     CDS             101..1174
                     /locus_tag="P5014_02225"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HRY56960.1"
                     /translation="MKIIIFAGGAGTRMWPLSRKSFPKQFIKMFKGKSTLELAINRVK
                     SFGYENIFVSTVKEYVTLTKKYLPKIPARNIIGEPALRNLAPAVGLNLINLRAKGYKG
                     PVAILWADHLIENETLFLETIKKAEEISLKNPDKIIFVGKTPRFANNNLGWIHFGKKT
                     SKDIFEYNKWFYKPPVEECEKMFKSKEWLWNTGYFFMDIDFGCYLYEKYQPEIYKKLL
                     LIEKSLGKGKEKEIVNKVYPTMEKISFDDAIAKNVLPSEALVLKTEMEWSDPGTLYAL
                     KEALTENEKDNLIMGNVKTLNTEDSLLVNHERQKLLATIGLRGVAVVNTKDVVLVVPK
                     ENVKEISDLLVEIEKDKNLKKYL"
     gene            complement(1258..1590)
                     /locus_tag="P5014_02230"
     CDS             complement(1258..1590)
                     /locus_tag="P5014_02230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56961.1"
                     /translation="MSFKNILKIFLLDWIVTVVMYLVGLLITGIGDLRPADIVFYLSN
                     LPSSPNGTSPLLDVLSYLMVSLGFVVLISYLVNKSYLKLSIREFVVAQVTYIISALLL
                     IFTVFLTL"
     assembly_gap    1772..1781
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1782..2155)
                     /locus_tag="P5014_02235"
     CDS             complement(<1782..2155)
                     /locus_tag="P5014_02235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56962.1"
                     /translation="MYAKLEEAVFVKELKSEINTFQNLKPSDKVILIAPNETILAHTI
                     EFIGGRNGVGDLSAVTSEMRARSSIGRIGIAVCKCAGWGDIGYINRWTMEITNFSSSV
                     IPLPVGLRIAQIIFHSTERIEG"
BASE COUNT          959 a          472 c          465 g          795 t
ORIGIN      
        1 atacagtctt tccaggactg cgcactagtc cactatgcga tccctccaag gctaaattag
       61 attttaccac gattacggac caaaaatgtt aaaatatctt atgaaaataa taatctttgc
      121 aggaggagca ggcacaagaa tgtggccact ttctcgaaaa tcatttccca aacagtttat
      181 aaaaatgttt aaaggcaaat ctacacttga acttgccata aacagagtaa aaagttttgg
      241 ctacgaaaat atcttcgtca gtactgtaaa ggagtatgtg acccttacta aaaaatatct
      301 accaaaaatt cccgcaagaa atatcattgg tgaacctgct ttaagaaatt tggctcctgc
      361 tgtaggttta aatttaataa atttaagggc aaaaggatat aaaggccccg ttgctattct
      421 ttgggccgat catctaattg aaaacgaaac acttttctta gagactataa aaaaagctga
      481 agaaatatcg ttaaaaaacc ccgacaagat aatatttgta ggtaaaacac caagatttgc
      541 aaataataac ttaggctgga ttcattttgg aaagaaaacc tcaaaagata tttttgaata
      601 taataaatgg ttttataaac caccggtaga agaatgtgaa aaaatgttta aatcaaaaga
      661 atggctttgg aatacagggt attttttcat ggatattgat tttggatgct atctatatga
      721 aaaatatcaa cctgaaattt ataaaaaact attattaatt gagaaatcat taggaaaagg
      781 taaggaaaaa gaaatagtaa acaaggtgta tccgacaatg gaaaaaatta gttttgacga
      841 tgctatagcc aaaaacgtgt taccaagtga agctttagtt ttaaaaacag aaatggaatg
      901 gtccgatccc ggaactttgt atgcattaaa agaagcttta acagaaaatg aaaaggacaa
      961 tctcattatg ggaaatgtca aaacactgaa tacagaagat tccctactgg taaatcatga
     1021 gaggcaaaaa ctactagcta caattgggtt aaggggtgta gcagttgtaa acacgaaaga
     1081 tgtggtttta gtagtaccta aagaaaatgt taaagaaata tccgatttat tagtggaaat
     1141 tgaaaaagat aaaaatctta aaaagtatct ttagacaagg agatgtgtta gtaataaaga
     1201 taatttagat agataacaag ctgtgaaata aaactgtatt catgaatctc ccgaatccta
     1261 cagtgttaaa aatacggtaa agatcagaag gagtgcagaa ataatataag taacctgtgc
     1321 cactacaaat tcccttatag ataattttaa atagctttta tttactaaat aacttatcag
     1381 aacaacaaac cctaaactaa ccataagata tgataagaca tccaaaagag gcgaggtacc
     1441 atttggtgaa gagggtagat tagataggta aaaaactatg tcagcgggtc ttaaatcccc
     1501 gattccggtt atcaacaaac caactaagta cataacaacg gtaactatcc aatctaagag
     1561 gaatatcttt aatatgtttt taaaagacat agcgtaacta ttttatcatt tactgaaaat
     1621 tttcggaatt atattttctc agaattcgca atctttgaaa attttcccaa atccctatcc
     1681 tcatataact taggaagcat gtcggaaggt ttccattttc tctttaaaac ctctaattca
     1741 gaattgagct ggtacttccc tccttcaaaa tnnnnnnnnn nttaccctca atcctctcag
     1801 tactgtgaaa tattatctgc gctattcgta aaccaacagg taaaggaatt accgagctgc
     1861 taaaatttgt tatctccata gtccatctat ttatataacc aatatctccc caaccagcgc
     1921 atttgcatac tgctatgcca attcttccaa ttgagcttct agccctcatt tccgaagtga
     1981 ctgcgcttaa atcacccaca ccgtttctac ccccaataaa ttctattgta tgagctaaaa
     2041 tagtttcatt aggagcaatt aaaataacct tatctgatgg ttttaaattt tgaaaagtgt
     2101 ttatctcaga tttaagctct ttaacgaaaa ctgcttcttc tagttttgca tacattttgt
     2161 tcaaggactc ctgtgaaaaa gggtttaata tctccgtatg tttaagagtt agctgcctat
     2221 aaaaatgact ccctaaccta acatcataac tagcattacc caggttacta ggactaaaag
     2281 ggtcaataac tatattgcct aatttgtaat gattaagtat ttcgggtttt gagagaacac
     2341 tcataggaga tattatagac tatttgttct tcagagaaag taattttaaa ttagtatata
     2401 aaagaaaaca ttttctaatc tctaacactt taaaaccggt ataggaaatt atctaaaagc
     2461 gcgtacagtt ctcactggcg gagggagaga gattcggtca ccgattctcg cctgctggct
     2521 cgaccgtcga ccccggctcg gcgccgtcgc gcctgcgcca ttcgaatctt tttggaaatt
     2581 cttttaagat atgtagtgat ttccaaacag cgacagtggt tctcactggc ggagggagag
     2641 agattcgaac tctcgtgtga cttgcgccac gcttggtttt cgagaccagc ccgttatggc
     2701 c
//
LOCUS       DAOTFA010000057         2679 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate AMR_MDS_3115
            NODE_30544_length_2679_cov_4.935595, whole genome shotgun sequence.
ACCESSION   DAOTFA010000057 DAOTFA010000000
VERSION     DAOTFA010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868139
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (wastewater metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2679)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2679)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:36:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 536
            CDSs (total)                      :: 521
            Genes (coding)                    :: 520
            CDSs (with protein)               :: 520
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2679
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30544_length_2679_cov_4.935595"
                     /isolate="AMR_MDS_3115"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..423)
                     /locus_tag="P5014_02240"
     CDS             complement(<1..423)
                     /locus_tag="P5014_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56963.1"
                     /translation="MGFDSYPNNISKSDDNHRTLWSSESYDTAKKAENDLFKTMKYIG
                     LREVTEDDQKSGQVFKEPVFRVSNKKEDEEEATDCFVYDPRNGRWNRVDLTTATNPEI
                     IRKKEIRHKKLESELGEEIKLLKIPGGVLDRASRHCERD"
     gene            483..2447
                     /locus_tag="P5014_02245"
     CDS             483..2447
                     /locus_tag="P5014_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56964.1"
                     /translation="MPNLKLSELKRYFKNSLFFLSVIFLSIPTFAGTTPPITTFEKEP
                     EIPDGNNDWYRQPVKITLSASDLESGVKEIFYRVDEGSWVKKDFSNSLNLAPNPSFEI
                     SSATPPLDTQDWKISNSNPGGTYTRDTLVYMPSFANTSIKINSTEDTWHSIDHYDMFA
                     AATSFNSMSAYVWIKTLSISGNAYFNIYSISQDSMGQITTNFIKSSPAVTGTNDWTKL
                     STNFTPTAENVIGVYIEIGFSGTGTIWIDAANISKSNLPITSFYVSTDGNHTVDYYAV
                     DKAGNIETTKSGSFKIDQTPPGNWKDSGAVRSLLGNAHQLYMWTNVEDKTSGLSVFTD
                     KFQYFIPGKSTGFGRYPLLGSCSGNWQSGGWGILVSPPFSPGVKEAYLITPMVDFCNS
                     EWHKACHYVRFYAEDMAGNSSTRDMCINGPWIRVRGEGIVRANQNIDMIAEVPSGEYN
                     TDGLIEVGEHSINFFNSSKNIAYYEAEVPEEYDYQKFFDTVRGEKTQISTSGNLISSS
                     GIYYINGNYEISGNKIPSNYSTSVFNQIVFVNGNLTISSNIAVSKQSTTLFIVKGNVN
                     IDEDVTAVSVGLISDQTIDTAYNFSDKGKRNSIEIKGILIANKVAFKRSLQGTGNKKF
                     PAEDITYEPKYVLKMSDYIGNNTVKWVYSD"
     gene            complement(2444..>2679)
                     /locus_tag="P5014_02250"
     CDS             complement(2444..>2679)
                     /locus_tag="P5014_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HRY56965.1"
                     /translation="ESKLNTILKMDDFLGLKLQEHIGVEIPKNVLDLAKMRGEYRKNG
                     IWDKADVLRRQVNDLGYVIDDIPNGDFKVKKKF"
BASE COUNT          893 a          510 c          421 g          855 t
ORIGIN      
        1 atctctctca caatgtcttg aagctctatc tagaacaccc cctggtattt ttaataactt
       61 tatttcttct cctaattccg attccaactt cttgtgacgt atttcctttt ttctaattat
      121 ctctggattt gtcgcagtcg ttaaatcaac gcgattccac ctaccgtttc tgggatcata
      181 aacaaaacaa tccgttgctt cttcttcatc ctccttttta ttagatactc taaaaacagg
      241 ttctttaaag acctgcccgc ttttctgatc atcttctgtt acctctctca aaccaatata
      301 tttcatagtc ttaaataagt cattctcagc cttctttgcc gtatcataag attctgaact
      361 ccacaaagtt ctgtgattat catcactttt cgaaatatta ttcggatagc tatcaaaacc
      421 cataaaataa tttaaccaca ccccacttca aaactccaca aaaataatct atgatatatc
      481 taatgcccaa tttaaaatta tccgaattaa aaagatattt taaaaattct ttgttttttt
      541 tatctgtaat atttttatca atacctacct ttgccggtac taccccacct ataacgacat
      601 ttgaaaaaga gcctgagata cctgacggta ataatgattg gtacagacaa cccgtgaaaa
      661 tcactctctc agcttcggat ctcgaaagcg gagttaaaga aatattttat cgtgttgacg
      721 aaggcagttg ggtaaagaag gatttttcaa actcactaaa tttagcccca aacccatctt
      781 ttgaaatatc atcagctacc ccaccgctcg acacacaaga ttggaaaatt agtaactcaa
      841 atcccggagg cacttatacc agagatactt tagtgtatat gccttctttt gctaacacat
      901 caataaaaat aaactcaacc gaagatacat ggcactcaat tgaccattat gatatgttcg
      961 ctgctgcaac ttcatttaac agtatgtcag cgtatgtttg gataaaaact ttaagtattt
     1021 caggaaacgc gtattttaat atttattcaa tatctcaaga ttcgatgggg cagattacta
     1081 caaattttat aaaatccagt cccgcagtta caggaacaaa tgattggaca aaactatcaa
     1141 caaattttac ccccactgca gaaaatgtta tcggagtata tatagaaatt ggtttttcag
     1201 gtactggtac tatatggatt gatgccgcta atataagcaa atccaattta ccaataactt
     1261 ctttttatgt aagcaccgac ggaaatcaca ccgttgatta ttatgcagta gacaaagcag
     1321 gaaatattga aacaacaaaa tcaggaagtt ttaaaattga ccaaacacct cccggtaact
     1381 ggaaagattc tggagccgta aggtcattac ttggaaatgc tcatcagcta tacatgtgga
     1441 ctaatgttga agataaaact tctgggttat cggtatttac agataagttt caatatttta
     1501 taccaggtaa aagcactggt tttgggcgtt atccactact aggtagttgc tcaggtaatt
     1561 ggcaatctgg tggttggggt attttagtat cacctccctt ttctccagga gttaaggaag
     1621 catatctaat tacaccaatg gtagatttct gtaacagcga gtggcataaa gcctgtcact
     1681 atgtcagatt ttatgccgaa gatatggcag gaaactcttc tacaagagac atgtgtataa
     1741 acggtccatg gatcagagtt agaggagaag ggatagtgag ggcaaatcag aatattgata
     1801 tgattgctga agttccttct ggagaatata acacagacgg tttaatcgaa gtaggcgaac
     1861 actcgattaa tttcttcaac tcctctaaaa atatagcata ttacgaagca gaggtcccag
     1921 aagaatacga ttatcaaaaa ttctttgata cagtgagagg agagaaaaca caaatatcta
     1981 cctcaggcaa cctaatttct tcatcaggta tttattacat aaatggaaat tatgagatta
     2041 gtggaaataa aataccttca aactacagta catctgtttt caatcaaata gtttttgtca
     2101 atggaaatct aacaatctct agtaacattg cagtatccaa gcaatcaacc acacttttta
     2161 tagtaaaggg gaatgtaaac atcgatgaag acgtaacagc tgtcagtgtc gggttaattt
     2221 cagatcaaac tattgatacc gcttataact tttctgacaa aggtaaacgt aattcgattg
     2281 aaattaaagg tatattaata gcaaataaag tcgcttttaa aagatcatta caaggaactg
     2341 gaaataaaaa attccctgca gaagatatta catatgaacc aaaatatgtt ttaaaaatgt
     2401 cggattatat tggaaataat actgtgaagt gggtttactc tgattaaaac ttctttttta
     2461 ccttaaaatc accgtttgga atatcgtcta taacatatcc aaggtcatta acttgtcttc
     2521 ttagaacatc agctttatcc cagattccgt tttttcgata ctcccctctc atttttgcaa
     2581 gatccaaaac atttttagga atttcgactc caatgtgttc ctgtaatttt aaacctaaga
     2641 aatcgtccat ttttaatatt gtatttaatt tggattctt
//
LOCUS       DAOTFA010000058         2654 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate AMR_MDS_3115
            NODE_31038_length_2654_cov_6.121970, whole genome shotgun sequence.
ACCESSION   DAOTFA010000058 DAOTFA010000000
VERSION     DAOTFA010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868139
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (wastewater metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2654)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2654)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:36:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 536
            CDSs (total)                      :: 521
            Genes (coding)                    :: 520
            CDSs (with protein)               :: 520
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2654
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31038_length_2654_cov_6.121970"
                     /isolate="AMR_MDS_3115"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..577)
                     /locus_tag="P5014_02255"
     CDS             complement(<1..577)
                     /locus_tag="P5014_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011498997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRY56966.1"
                     /translation="MSIIKIENLKRTYGTKVLTHALKGVSFEVSEGDFIIIMGKSGSG
                     KSTLLHQLGLLDTPTSGEIFIKNENTKDLSEKKKTIFRLEKLGYIFQEYAILPELTAL
                     ENVYLPLMVSSNNGNKDFKEKAKEMLKLVGLEHRFDHYSNELSGGEQQRVAIARALVN
                     NPQVLFADEPCANLDTQSSKNILDLLKDLNKK"
     gene            complement(581..1798)
                     /locus_tag="P5014_02260"
     CDS             complement(581..1798)
                     /locus_tag="P5014_02260"
                     /inference="COORDINATES: protein motif:HMM:NF014717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HRY56967.1"
                     /translation="MEKFKTAFFLAYKAIVKGNIGTTTLTISVMVLAFVNLLFLSSIL
                     LGLVNTMNRQGVNNLYANIVIEPASQDNLISNASRVMTTVNGVPGVLGSARHYKIGGF
                     FTFDRYKNNQDVMSGQYSVISIDPNKEKEITNISKSISFGSYLEENDRNKIVIGKDIA
                     GGQFGSFEKDSLGGVKIGDEITISFENGVTRVYEVKGIFNSKFVQSDKAAFITNDEME
                     SILGVANAAHEIIVKTEEGVPESYYMEKFKSMGIKEDIYPWTDYMGMTESFTKSFTLI
                     RTLLSAIALLVAGITIYIVIYINVLNNKKQIGILRAIGIDKKIILISYTVQALFYALS
                     GIIIGLAVLSFILVPYFQGHPLEFPVGDVHLEIVKAEIILSAVSLTIAGFISGFIPSR
                     QVTNKTILDSIWG"
     gene            complement(1785..2168)
                     /locus_tag="P5014_02265"
     CDS             complement(1785..2168)
                     /locus_tag="P5014_02265"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HRY56968.1"
                     /translation="MKNQQGKKEKNFKISYPEQGLLQLLILKFIYGNPSYGYDLIKQI
                     ADISNNSMKIKSGTMYTTLRRMEKSGFIKSVWREGDIGPSKRLYGLTPQGKRYLNKML
                     HNISSRIPIVQELLSFYKKELDGKI"
     gene            complement(2339..>2654)
                     /locus_tag="P5014_02270"
     CDS             complement(2339..>2654)
                     /locus_tag="P5014_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56969.1"
                     /translation="RHRSEKPAMWVRLPPSPPGNLACGFRYRRDFLMGRGEELASSEF
                     MSVKRAASHISAKRKFMRKRRNEVTSKRLYCEAIQEEAQERSDEQATILRSNTRGSAG
                     TK"
BASE COUNT          782 a          519 c          405 g          948 t
ORIGIN      
        1 actttttatt taaatctttc aacaaatcta aaatattttt tgatgattgt gtatccagat
       61 ttgcacaggg ctcatcagcg aacaaaacct gaggattatt tactaaagct cttgcaattg
      121 ctaccctttg ttgttctcct cccgaaagct cattagaata atgatcaaat ctgtgttcca
      181 aacccacaag ttttagcatt tcctttgctt tttctttaaa atctttattt ccgttatttg
      241 agctaaccat caatggaaga tatacattct ctaacgctgt taattcgggc agtatcgcat
      301 actcttgaaa aatataacca agcttttcta atctaaatat tgtcttcttt ttttcgctaa
      361 gatctttagt attttcattt ttaataaata tctctcctga agtgggtgta tctaagagtc
      421 ccagttgatg aagtaaagtt gattttcccg aaccactttt acccattatt ataataaaat
      481 caccctcaga tacctcaaaa gagacaccct ttagtgcgtg tgtgagaact ttagttccgt
      541 atgttctttt taaattctct attttaataa ttgacataaa ttaaccccaa atagaatcca
      601 aaattgtttt attggtaact tgccttgaag gtataaaccc tgatataaat ccggcaatag
      661 ttaaacttac agcgcttaaa ataatttctg ctttgactat ttccaaatgt acatccccaa
      721 ccggaaattc taaggggtgt ccttggaagt aaggcactaa aataaaactt agaacagcca
      781 acccaataat tattccggaa agggcgtaaa acaatgcttg aacagtgtaa gaaataagta
      841 ttattttttt atctatccct attgcccgca aaatcccgat ttgttttttg ttattaagta
      901 catttatata gataacaata taaattgtaa ttcctgctac aagaagggct attgccgata
      961 aaagtgttct gattagagta aagcttttag tgaagctctc cgtcatgccc atatagtctg
     1021 tccatgggta gatatcttct tttataccca ttgatttaaa cttttccatg tagtagcttt
     1081 caggtacccc ttcttctgtt tttacaatga tttcatgtgc agcattagct acccctaata
     1141 ttgattccat ttcatcattt gtaataaacg cagctttgtc cgactgaaca aatttagaat
     1201 taaaaattcc tttaacttcg tatactctgg ttacaccatt ttcaaaagaa attgttattt
     1261 catcaccaat ttttacacct cccaaagaat ccttttcaaa agaaccgaat tggcctcctg
     1321 ctatgtcttt ccctatcact atcttatttc tatcgttttc ttcgagataa cttccaaaag
     1381 atatggattt acttatattc gtaatctcct tttctttgtt cggatcaata gaaatcactg
     1441 aatactgacc ggacataaca tcttgattgt ttttgtatct atcaaaagta aaaaaacctc
     1501 caattttgta gtgtcttgcg ctacctaaaa caccgggaac accgtttaca gttgtcataa
     1561 ctcggctcgc gtttgatatt aaattgtctt gactagccgg ttcaattacg atgtttgcat
     1621 agagattatt tacaccctgt ctgttcatag tgttaacaag cccaagtaaa attgaggata
     1681 aaaataaaag gtttacaaaa gctaatacca taactgatat ggttaaagtt gttgtaccaa
     1741 tattaccctt gactattgct ttgtaagcga gaaaaaacgc tgttttaaat ttttccatct
     1801 aattcttttt tataaaaact aagtaattct tgtacaatag gaattctact actgatatta
     1861 tgaagcattt tgtttaggta tcttttacct tgtggtgtta gcccatacaa tcttttagaa
     1921 ggacctatat caccttccct ccaaacagat ttaataaaac cagacttttc cattcttctt
     1981 aaagttgtgt acatggtacc tgactttatt ttcatagagt tgttactaat atcggcaatc
     2041 tgtttaatta ggtcatagcc gtagcttgga ttgccgtaga taaatttgag tatcaggagt
     2101 tgtagcaatc cttgttcagg ataactaatt ttaaaattct tctctttttt tccctgttga
     2161 tttttcattc tatataacat tgttacatag agttaagata attacaatat agaagtttaa
     2221 aaaagttggt gtgaaaatgt aaaattgata aatataaaaa aaatttacat tctttaaata
     2281 ttgttataac aacaatccca aaggtctggt ggggagggtg ggagtaagag cttgctcgtc
     2341 acttcgttcc tgcgcttcct cttgtattgc ttcgcaatat agtcgcttgc tcgtcacttc
     2401 gttcctgcgc ttcctcttgt attgcttcgc aatatagtcg cttgctcgtc acttcgttcc
     2461 tgcgcttcct cataaatttt cgctttgctg aaatgtgcga agccgctcgt ttaacgctca
     2521 taaattcgct actcgctagt tcctcacctc ttcccataag aaaatctcgc ctgtacctaa
     2581 agccacacgc gagatttcct ggtggggagg gtgggagtcg aacccacata gccggttttt
     2641 cagaccggtg cctt
//
LOCUS       DAOTFA010000059         2553 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate AMR_MDS_3115
            NODE_33095_length_2553_cov_7.646117, whole genome shotgun sequence.
ACCESSION   DAOTFA010000059 DAOTFA010000000
VERSION     DAOTFA010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868139
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (wastewater metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2553)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2553)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:36:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 536
            CDSs (total)                      :: 521
            Genes (coding)                    :: 520
            CDSs (with protein)               :: 520
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2553
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33095_length_2553_cov_7.646117"
                     /isolate="AMR_MDS_3115"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..431
                     /locus_tag="P5014_02275"
     CDS             <1..431
                     /locus_tag="P5014_02275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HRY56970.1"
                     /translation="MGLDVVKELKKRTNVIVPDIKTKDKDVDSTLIEYAKEKKIKLMT
                     MDFNLNKVAKVSGITVLNLNELINALKTVLLPGERFGVKIVQEGKEKKQGIGYLNDGT
                     MVIVEDGIEKVGQDIEAVVIKVIQSSAGKIVFSEVAKNGN"
     gene            774..917
                     /locus_tag="P5014_02280"
     CDS             774..917
                     /locus_tag="P5014_02280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56971.1"
                     /translation="MKNPKEKVKTTIMIEKTLKKFAQVYAVQNDMTLGEVIEEALRDC
                     LNK"
     gene            complement(1225..1503)
                     /locus_tag="P5014_02285"
     CDS             complement(1225..1503)
                     /locus_tag="P5014_02285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018213696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRY56972.1"
                     /translation="MKFRDFNEIKKELLSDPEVKKEYDSLGPKFDLIRSIIDKRIEKN
                     ISQEELASRMGTKQSALSRFESGNYNPSLAFMQKVAQALDSKLEIRLR"
     gene            complement(1919..2206)
                     /locus_tag="P5014_02290"
     CDS             complement(1919..2206)
                     /locus_tag="P5014_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005377969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRY56973.1"
                     /translation="MKKNYGYPFEKHLKEQLKNPAFKKAWDEIEIESSIAEGMIGKRI
                     EKNISQRDLAKQINSTQAVISRIESMNANPSIKTLKKIAKALDAKLEIKIR"
BASE COUNT          795 a          364 c          483 g          911 t
ORIGIN      
        1 ggatggggct ggatgtggta aaagagttga aaaaaaggac aaacgttatt gttcctgata
       61 ttaaaacaaa ggataaggat gttgactcaa ctttaattga gtacgcaaaa gagaagaaga
      121 taaagctaat gacaatggat tttaatttaa ataaagtggc gaaggtgtcg ggaattacag
      181 ttttaaattt aaatgagttg attaatgctt taaaaactgt gcttttacct ggtgagaggt
      241 ttggtgtaaa aattgttcaa gagggaaagg aaaaaaagca ggggatcggg tatttaaatg
      301 atgggactat ggttattgtt gaagacggaa ttgaaaaggt cggacaggat atagaggctg
      361 tagttataaa ggtaattcaa tcatcggcgg ggaaaattgt gtttagtgag gttgcaaaga
      421 acgggaatta aaaaaacatt ccacaagaag cgacatataa agcgctggcc gcagaatgtt
      481 tttagaagcg tgattccgtc aaagacggaa tgagatcatg cccccgagca ggcgaagcat
      541 gaaagcttac ttgaaatata atttatacca acgaaatttt ttaacaagcg acgagcgttg
      601 agaaatcatc cttgaaaaat catcacagca gagaaaattc atttccattc taatgtgcat
      661 tgttaattca tatgtattta catatatacg tatatatggt aaattataaa tattagttat
      721 cctataaggt atttatttgt tgtggataac ttaaaaattt gtggagaact tatatgaaaa
      781 accctaaaga gaaggtaaaa accactatca tgatagagaa gactttaaaa aaatttgccc
      841 aggtttatgc agttcaaaat gatatgactt tgggagaagt tatagaagag gctttgagag
      901 attgtttgaa taaatgatct aaaaaattag cagttggata taaaaaaaga agatagaaac
      961 gaagaaggcc tccttacgga ggccttctta tctagctgta ccttggttag cagacgtcga
     1021 tgttattatt ctattacgca ttgtattgtt ggtcaatagg ttttcttggt ggagcggacg
     1081 gggattgaac ccgcaaagct agagtctcga cgtctgctcg cctaccaagg cccgccccat
     1141 caagaatatt tatattcttt gtgtttgatt ttcgtaagtt aggattatct attcagagta
     1201 gataattata atgaggggag agttctatct aagtctaatt tccagttttg aatctaaagc
     1261 ttgagctacc ttttgcataa aagctaatga aggattatag tttccggatt caaatcttga
     1321 tagggcgctt tgttttgtac ccattctact tgcaagttct tcctgactaa tatttttctc
     1381 aattctttta tctattattg atctaataag atcaaatttg ggaccgaggc tgtcgtactc
     1441 cttttttact tcagggtcgc tcaataattc ttttttaatt tcattaaaat ctctaaactt
     1501 catattttac ttatataaca tatatgttat atgtcaaggt gttttggttt atttaatcat
     1561 ttcttttttg cttataaata tttgtttgga ttatttttgt aacagataag gtcagaaatt
     1621 aggtaaggag ataaattttg taaaggcgaa acgaagaagg cctccatacg gaggccttct
     1681 tgtctagctt taccttggta agcagatgtt gactcttaca ttctattact tgttgtaata
     1741 ttcgtcaata gagtttcttg gtggagcgga cgggaatcga acccgcaaag ctagattgtc
     1801 aacatctgct cgcctaccaa ggcccgcccc atcaagatta tttatattct tgaaatatga
     1861 tttcttaaag tgatcggtgt ctatttagag tagaatgtgt tgaaatttat atattttttt
     1921 acctaatctt tatctctagt ttggcatcta aagccttggc aatctttttt aaagttttta
     1981 ttgaagggtt tgcattcata ctttctattc ttgagattac agcttgagtg ctgtttatct
     2041 gtttagccag atccctttga gatatattct tttcaattct ttttccaatc attccttctg
     2101 ctattgatga ttcaatttct atttcatccc aggctttttt gaatgccgga ttttttaatt
     2161 gttcttttaa atgtttttca aatgggtatc cgtaattttt tttcatttta ataatatatc
     2221 ttttaagata tcaattgtca aattgatttt tttatttctt ttagaatatt tatttaactt
     2281 aaaatcaaca ttaacaaaca tcttcagccc tatagtgttg acaaagttga aaaaatagta
     2341 taaaattctt tcattacaga gttggagaga gactatgtct gtactattct tttagttttt
     2401 cctcctaccc gtgtaaatgg ttcttgctct tattttttta aatgagagta aaaactagct
     2461 ttttagcaat agctaaaaag aagagtactt tgacaatatg tgcggaaaca ttctccttta
     2521 ttttagttct cgtgcacctt tttgattata ttt
//
LOCUS       DAOTFA010000060         2534 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate AMR_MDS_3115
            NODE_33467_length_2534_cov_5.638967, whole genome shotgun sequence.
ACCESSION   DAOTFA010000060 DAOTFA010000000
VERSION     DAOTFA010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868139
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (wastewater metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2534)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2534)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:36:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 536
            CDSs (total)                      :: 521
            Genes (coding)                    :: 520
            CDSs (with protein)               :: 520
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2534
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_33467_length_2534_cov_5.638967"
                     /isolate="AMR_MDS_3115"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            157..753
                     /locus_tag="P5014_02295"
     CDS             157..753
                     /locus_tag="P5014_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015327641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HRY56974.1"
                     /translation="MNNTLVPIVIEKDQSGIERSYDIYSRLLKDNVVFVTGPIDMGVA
                     NTFIAQMLFLESQNPDKDISVYINSPGGEVYAGVAMYDTIKHIKNDVVTINVGLAASA
                     AALILSSGTRGKRYALPGSHTMIHQVIGGVEGQASDVEITAKHMLKLRDQYAEAIAKE
                     TGQKLDKVKGDMDRDYWMDAGESVKYGIIDKVMNPKKK"
     gene            1140..1216
                     /locus_tag="P5014_02300"
     tRNA            1140..1216
                     /locus_tag="P5014_02300"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1174..1176,aa:Val,seq:gac)
     gene            complement(1670..1969)
                     /locus_tag="P5014_02305"
     CDS             complement(1670..1969)
                     /locus_tag="P5014_02305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56975.1"
                     /translation="MEEGNKSLIMGLWKFHERVGEKELIEVANEWAEDNKYLQLYIRK
                     VSKDQNGIGFAYLSDGSREAHSTYFDKVSDALKRKFGNDLVGWDISSSSTLIKGF"
     gene            complement(2212..>2534)
                     /locus_tag="P5014_02310"
     CDS             complement(2212..>2534)
                     /locus_tag="P5014_02310"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007062190.1"
                     /note="catalyzes the formation of phosphoenolpyruvate from
                     pyruvate; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HRY56976.1"
                     /translation="TQMTYGFSRDDAGKFLGDYVEKKILPEDPFKTLDIVGVGKLMKM
                     AVDLGRTTKKHLHIGICGEQGGDPATIKFCHEIGLDYVSCSPYRVPIARLSAAQAAIE
                     NEKI"
BASE COUNT          881 a          474 c          426 g          753 t
ORIGIN      
        1 atccgatgca aagactttat atcaaaacta ttttgcaaaa aaacaaactt ttaaacaatt
       61 taattgatga aacacaaggt cctcatgacc acgaccatca tcacgaagag agtgtggttg
      121 aggaatcaaa agaggaacct aaaaaggagg ataaccatga ataatacctt agttccaata
      181 gttatagaaa aagaccaatc aggaatagaa aggtcttatg atatttattc aagactttta
      241 aaagataatg tggtttttgt gaccggacca attgatatgg gtgtggcaaa tacctttatt
      301 gctcaaatgc tttttctaga aagccagaat cctgataaag acattagtgt ttatattaac
      361 tctccgggag gagaagttta tgcaggagtt gcaatgtacg acaccattaa acatataaaa
      421 aatgatgtgg ttacaataaa tgttgggttg gctgcatctg ctgcagcatt aatactatct
      481 tcaggaacaa gaggaaaaag atacgcgctt ccgggatcac acaccatgat tcaccaagtt
      541 attggcgggg tagaggggca agcgagtgat gttgaaataa ccgctaaaca catgttaaaa
      601 cttagagacc aatacgcaga agcaattgca aaagaaacag gtcaaaaatt agataaagta
      661 aaaggagaca tggacagaga ttattggatg gatgctggag aatctgtaaa atacggaatt
      721 attgataaag taatgaatcc taagaaaaag tgaggagttg aaaatagaat aaaataatac
      781 ttcgggcggt tagctcctga tggagcaaag cgacatccgg agatagccct acactcaagt
      841 agcttaagat attaaaggcg agccttggcg agcgaaacat tttgaaaata aaataaaatt
      901 atacctgggg cggttagctc ctgtttgaag aaccggagat agccctacac acaagtaatt
      961 taaggtttta gaaacgagct ttagcgagtg agataagttg aaaataaaat aaaataatac
     1021 ttcgggcggt tagctcctgt ttgaagaacc ggagatagcc ctacacacaa gtaatttaag
     1081 gttttagaaa cgagctttag cgagtgagat aagttgaaaa taaaataaaa taatacttcg
     1141 ggcggttagc tcagtcggtt agagcgccac attgacattg tggaggtcat tggttcgatt
     1201 ccattaccgc ccaccaaaac taagaacttt ttattaaagt tattggtata aaaaagttga
     1261 tgttctaaga cgagcaccca cttattgtat ttttacatac aactaaatct ttttttcacc
     1321 aacctctttt ctcttttctc atttcctccg tatgttctag aaaatataaa tttgcaaaac
     1381 cagataaaaa cccaaaaaaa caaattaaga tggttattga tataacttgg tcctataaac
     1441 ctgaatccaa aaaaccttgc tttgcttctt ttgaaccatt aaaagttatt ccaagcagta
     1501 ccattactac tactgcacca actaaaaaaa ccatccagaa attttttttc accacaacct
     1561 ccgtttgtgt gatacaagac ttgttatata tctccgacta atttggtcag aaacacatta
     1621 catttctgta tcccagtccc aattggttac ttcccacccc aaatggttat taaaaccctt
     1681 taatcagagt ggatgaacta gaaatatccc aacctactaa atcgttacca aattttcttt
     1741 taagagcatc ggaaacttta tcaaaatatg tgctgtgtgc ttctcttgaa ccatcgctta
     1801 aatatgcaaa cccaattccg ttttgatctt tacttacttt tctaatatat agttgtaaat
     1861 atttgttatc ttctgcccac tcgtttgcta cctcaattaa ttctttctct cctacacgtt
     1921 cgtgaaactt ccaaagaccc attattaaag atttattacc ttcttccatt aaaactcacc
     1981 ccctttaaat acaaaaatcc cacggatcct ttccatgggt attagaacac taaaaaatta
     2041 tttacttgta gcagttgaaa ctgctggaaa gaaaaggaat ggaaagtaac catcattatt
     2101 gtaacataga ttgttgtttt gtaaagctac taaatgttgt tataaaaaca ataacacaaa
     2161 ccatctataa aaaatttaac ccgctacaat taaatcattg tgccttaatg tttatatttt
     2221 ttcgttctct atggctgcct gagctgcaga caatctagca attggaacac gatatggaga
     2281 acaagaaacg tagtccaatc ctatttcatg acaaaatttt attgtcgcag gatccccgcc
     2341 ctgctcaccg caaataccaa tgtgcaaatg ttttttagtt gttcttccaa gatcaaccgc
     2401 catcttcatt aattttccta caccaacaat atccaatgtc ttaaatgggt cttcaggtag
     2461 aattttcttt tcaacgtagt ctcctaaaaa ttttcctgcg tcgtctcttg aaaaaccata
     2521 agtcatttgt gtta
//
LOCUS       DAOTFA010000061         2504 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate AMR_MDS_3115
            NODE_34106_length_2504_cov_8.032666, whole genome shotgun sequence.
ACCESSION   DAOTFA010000061 DAOTFA010000000
VERSION     DAOTFA010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868139
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (wastewater metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2504)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2504)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:36:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 536
            CDSs (total)                      :: 521
            Genes (coding)                    :: 520
            CDSs (with protein)               :: 520
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2504
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34106_length_2504_cov_8.032666"
                     /isolate="AMR_MDS_3115"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="P5014_02315"
     CDS             <1..227
                     /locus_tag="P5014_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015379891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="UvrB/UvrC motif-containing protein"
                     /protein_id="HRY56977.1"
                     /translation="VKPLREKLIDEVIEEQIIGKKEAWQEIDYKQLPPIELKKEIKKL
                     EEMMKYEAQVLNFEKAATLRDKIREIKKSV"
     gene            354..>2504
                     /locus_tag="P5014_02320"
     CDS             354..>2504
                     /locus_tag="P5014_02320"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HRY56978.1"
                     /translation="MQYLNKFLEFVEFTGNSILYVLKLLLALLKNIFSSLFIRSKKVP
                     KEYQDKHKKPRLFTFFVFIKDTIISVIFWIFRLIKDIVLFPKKIFTFLFLRKSFLSGI
                     LFAIFFIFLPITIIRWFKELPSPESLVIEANKKSTVILDRNGKLMYEIYVDKNYHPVD
                     FEEIPEYVKNATLAVEDDKFYSHFGIRFDSVIRAIKNNLSKENGLQGGSTITQQLVKN
                     VLLTPERTFSRKIKEAVLAVLVEARYSKKDIFEMYLNNIPYGGTAWGVQTASKKYFNK
                     DVWELSLAEASLLAGLPTAPTSYSPATDLETAKSRQRFVLDRMLNLGYINKEEFNKAI
                     SEELVFADTDGYIRAPHFVDYIRKDLEKRYGRRYVEFGGLTVTTTLDLDIQDKVQSIV
                     TEEVNKNSYLGFSNGASVVMDVKNAEVLAYVGSIDYFKEGWGAYDVVTAMRQPGSSIK
                     PVTYGLALSGSFTPASIIDDSPVTYKFKGSKDYTPVNYDGQYHGKVTLRQALANSYNV
                     PAVKVANVVGVDNIVLKGREMGLTNWEVDGSYGLSITLGGKEVRLLDHTNVYATLARQ
                     GVYKEPEVYISIKDSNGYDIYENENTKKTVLSPEVSYLLWDILSDQNARLPAFGVNNF
                     LSIPGHKIAVKTGTTNNIKDNWTLGYTPSYVVGVWVGNNDGTPMRQGLASGLTGAAPI
                     WNKIMTEMLKEKPNELYERPANVFVKKDDRCGISE"
BASE COUNT          875 a          357 c          481 g          791 t
ORIGIN      
        1 tagtaaaacc gttgagagaa aaacttatag atgaggttat cgaagagcag attattggaa
       61 agaaagaagc ttggcaggag atagattaca aacagctacc accgatagag ttgaaaaaag
      121 aaataaagaa actcgaggag atgatgaagt acgaggccca agtattgaat tttgaaaagg
      181 ctgcaacttt aagagataaa ataagagaga ttaagaagtc tgtttgattt taagtcttat
      241 aggtagagaa aattcgaatt attattcaca tatttgctaa cgtcggttat atattattga
      301 ctgcctatat ttttaataat atattggcat taactccacc cgtataaata attatgcaat
      361 acctgaataa atttttagaa tttgttgaat ttacaggaaa ttctatactt tatgttttaa
      421 aattactttt agcactatta aaaaatattt tttcctcttt atttattaga agtaaaaaag
      481 ttccaaaaga atatcaggat aaacataaaa aacctcggct ctttacattt tttgttttta
      541 taaaagacac aataatatcg gtaatctttt ggatattcag attgattaaa gatatagtcc
      601 tttttccaaa aaaaatattt acattccttt ttttaagaaa gtcttttctg tctggaattt
      661 tatttgcaat tttttttatt ttcttaccaa ttaccattat tagatggttt aaagaattac
      721 cctctccgga atctttggta attgaggcaa acaaaaaatc gactgtaatt ttggatagaa
      781 atggaaaact aatgtacgag atttacgttg ataaaaatta tcaccctgta gattttgaag
      841 agattcctga gtatgttaaa aacgctacat tagcagttga agatgataaa ttttacagcc
      901 attttggtat acgttttgat agtgtaatca gggcaattaa aaataattta tcaaaagaaa
      961 atggtttaca aggtggttct acgataaccc aacagttagt gaaaaatgta cttctcactc
     1021 ctgaaagaac tttttccaga aagattaagg aagccgtttt ggctgttttg gttgaagcaa
     1081 gatattcaaa aaaagatata tttgaaatgt atttgaacaa cattccttac ggcggaacag
     1141 cgtggggagt acaaaccgcc tccaaaaaat attttaataa agatgtgtgg gaattatcct
     1201 tagcagaagc ttctttgctc gcaggacttc ctaccgcacc cacaagttac tcacctgcca
     1261 cagaccttga aactgcaaaa tcaagacaga ggtttgtttt ggatagaatg cttaatttag
     1321 gatacataaa taaagaagag tttaacaaag ctatctccga agaattagtt tttgctgata
     1381 ccgatggata tatccgggca ccccattttg ttgattatat tagaaaagat ttagaaaaaa
     1441 gatatggaag aagatatgta gagttcggcg gtcttactgt gaccactact ttggatctcg
     1501 atattcaaga taaagttcag tcgatagtaa ctgaagaagt aaataaaaat tcatatttag
     1561 gattctctaa cggtgcttcc gtagttatgg atgttaaaaa tgcggaggtt ttggcgtatg
     1621 ttggttctat agattatttt aaagaaggtt ggggagctta tgatgtagtt actgcaatga
     1681 gacaaccagg ttcttcaata aaacctgtaa cttacggatt agctttatca ggtagtttta
     1741 ctccagcttc aattatcgat gactctccag ttacatataa atttaaagga tctaaagatt
     1801 acactccagt taattatgat gggcagtatc atggaaaagt tacactaaga caagctttag
     1861 caaactcata taatgtccca gctgtaaaag tagctaatgt tgttggggtt gataatattg
     1921 ttttaaaagg tagggagatg ggtcttacca attgggaggt agacggatct tacggacttt
     1981 cgataacgct cgggggaaag gaagtgagat tgttagacca tacgaatgtt tatgcaactt
     2041 tagctcgtca aggtgtttat aaagaaccgg aggtctacat aagtataaaa gattctaatg
     2101 gatatgatat ctacgaaaat gaaaatacta aaaaaacagt attatctccc gaagtttctt
     2161 atttattgtg ggatatttta tctgatcaaa acgcacgact cccggctttc ggtgttaata
     2221 atttcctttc aattcccgga cataagattg ctgtaaaaac aggcacaacc aataacatta
     2281 aagataactg gacattaggt tacaccccgt cttatgtagt gggagtttgg gtaggtaata
     2341 atgacggtac tccgatgagg caaggtttgg cctccggtct tacaggcgct gctccgattt
     2401 ggaataagat aatgacagaa atgttaaaag aaaagccaaa tgaattatat gaaagacctg
     2461 ctaatgtgtt tgtaaaaaaa gatgacaggt gtggaatttc ggaa
//
LOCUS       DAOTFA010000062         2457 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate AMR_MDS_3115
            NODE_35127_length_2457_cov_7.426311, whole genome shotgun sequence.
ACCESSION   DAOTFA010000062 DAOTFA010000000
VERSION     DAOTFA010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868139
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (wastewater metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:36:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 536
            CDSs (total)                      :: 521
            Genes (coding)                    :: 520
            CDSs (with protein)               :: 520
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_35127_length_2457_cov_7.426311"
                     /isolate="AMR_MDS_3115"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..324
                     /locus_tag="P5014_02325"
     CDS             <1..324
                     /locus_tag="P5014_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004511703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56979.1"
                     /translation="WFEKAKLCHKFHDPKLNFFEINASQPLSVWQKKGWIYEEDPRGW
                     FQWYCRYYMGRRIPEEDKRQIKRWRAIKRHIGQIKKNCAENDLDCRRKQRQALLHWAY
                     DSRKI"
     gene            complement(349..1116)
                     /locus_tag="P5014_02330"
     CDS             complement(349..1116)
                     /locus_tag="P5014_02330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56980.1"
                     /translation="MNIKDLFKIKLPFIFLFVVSLFLIFSDFVLLQSIGKKEEVLGAS
                     KRAGAESGTVRNRISVSSADNIPDVGAHISKVKNSALEIKKIAKAEKESGDNETGDEV
                     IKSVDDITYNSIDNIKSLEEIDKRPAWKNFLLGPDYKNLGKIRSNLVQNRNQIRKIEK
                     TIEKNKDEEDANTLRARLGELEQERLKIFNYIDEKDDGFSLFGWLTKLMFGYNKSIEE
                     EEDLGEEVEIGEDTDVSSDENKNDEEPKNTEDTELAE"
     gene            complement(1141..1608)
                     /locus_tag="P5014_02335"
     CDS             complement(1141..1608)
                     /locus_tag="P5014_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56981.1"
                     /translation="MKRNNLAFLIIILLSVGFLYLGFFSLKSNEKVCPASKKCDECVC
                     NEDKKEQTHAINAVGENNPIFIYGTLNKEEIPQELELGEYWYWLYFEKPLLLVNNASG
                     VPMYVDKIQVLPPATKDFYDIDSFVGKKVELYGYQTWGYAESSVFQINAVREY"
     gene            1745..2416
                     /locus_tag="P5014_02340"
     CDS             1745..2416
                     /locus_tag="P5014_02340"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HRY56982.1"
                     /translation="MKKTEKKTNIKEEEVSTKEVKVKKSIFGWFANNKPFIIAILIIM
                     AAFLSGYFIKNSLIAATVNGKTIWRYSLVRQLEKYYGSNILNTTIEQELVKQEAKNKK
                     VEVTKEEVDAKIKEIETSMASSGQTLDEALKESGMTRKDLEENYRLNLLIEKILSERI
                     QVTDEEVQKYIDDNSESFPEGTDIESVKTIIVEQLKQQKMSTEYQSFITELKEKADIK
                     TVAKY"
BASE COUNT          911 a          429 c          344 g          773 t
ORIGIN      
        1 tggtttgaaa aagcaaaact atgtcataaa tttcacgacc ctaaactgaa cttttttgaa
       61 ataaatgcct cccaaccatt aagtgtttgg caaaagaagg gttggattta cgaagaagat
      121 cctagaggat ggtttcaatg gtattgtcgc tattacatgg gaagacgaat tcccgaagaa
      181 gataaaagac aaataaaaag atggagagcc ataaaaagac atattggaca aattaagaaa
      241 aactgtgctg aaaatgattt ggattgcaga agaaaacaac gccaagccct attacactgg
      301 gcttacgaca gcagaaaaat atagactaat ctcttctcta aaaatggttt attcagctaa
      361 ttcggtatct tcagtatttt tcggttcttc atcgttctta ttttcatctg agctcacatc
      421 cgtgtcttca cctatctcaa cttcttcacc caaatcttct tcctcttcaa tacttttgtt
      481 gtacccgaac attaatttag ttaaccagcc aaacaaacta aaaccatcat ctttctcatc
      541 tatatagtta aaaatcttta atctctcttg ttcaagctct cctaaccttg ctcttaaagt
      601 attagcgtct tcttcatcct tatttttttc tatagttttt tcaatctttc ttatctgatt
      661 tctattttga accaaattag atcttatctt acctaaattt ttataatcag gacctaacaa
      721 aaaattcttc caagcaggtc tcttatcaat ctcttctaag gattttatat tatcaattga
      781 attgtaagtg atatcatcaa cggatttaat aacctcgtca cctgtttcgt tatccccaga
      841 ttccttctct gctttagcaa ttttttttat ctccaatgcg ctgtttttta cttttgatat
      901 gtgagctcca acatccggaa tattatcagc actactaaca cttatcctat ttcgaactgt
      961 accactctca gccccagctc ttttacttgc acccaaaact tcttcttttt ttcctatact
     1021 ctgtaataaa acaaaatcag agaaaattaa aaacaacgat actacgaaaa gaaatataaa
     1081 aggtaattta attttaaata aatctttaat gttcataaaa attcctttaa taaattaaaa
     1141 ttaatactcc ctaactgcat taatttgaaa aaccgaactt tcagcgtatc cccatgtttg
     1201 ataaccatat aactcaacct tctttcctac gaaactgtct atatcataaa aatctttagt
     1261 tgctggaggt aacacttgaa ttttatcaac gtacatagga acccccgaag cattattgac
     1321 cagtaataaa ggtttttcaa aatataacca ataccaatat tcacctaact ccagctcttg
     1381 tggtatttcc tctttattta aagtcccgta tataaatatt gggttgtttt cacctactgc
     1441 atttatagca tgggtttgct cctttttatc ttcattacaa acacactcat cacacttttt
     1501 agaagccggg catacttttt catttgattt taaagaaaaa aagcctaaat atagaaaacc
     1561 gacggagagt aatatgatta ttagaaaggc aaggttgttt cttttcatat aaatatttta
     1621 acacttttac acacctcaag tgtaatctca aaatcaaagt agtattataa aaagtataca
     1681 catgcccaga aagaaagcat tgttaaatgt tcgaagtatt tagttattat gctatacttg
     1741 gccaatgaaa aaaacagaaa agaaaactaa tataaaagaa gaggaagtga gtactaagga
     1801 agtaaaggta aaaaaaagta tttttggatg gtttgcaaat aacaagccgt ttattattgc
     1861 catcttaata ataatggcag cgttcttatc aggatacttt ataaaaaatt cattgattgc
     1921 tgcaacagta aatggaaaaa caatctggag atactctttg gttagacaac tagagaaata
     1981 ctacggatca aatatattaa atacaaccat agagcaagaa ttagttaaac aagaagccaa
     2041 aaacaaaaaa gttgaggtaa ctaaagaaga agtagatgca aaaataaaag agattgaaac
     2101 aagtatggct agctcaggac aaaccttaga cgaagcttta aaagaaagtg gaatgacaag
     2161 aaaggatttg gaagaaaact atagattaaa tttattaatt gaaaaaattc tatccgaaag
     2221 aattcaagtt actgacgagg aagttcaaaa atatatcgac gataattcgg agtctttccc
     2281 tgagggtaca gacatagaat cagtaaaaac aataattgta gaacaattaa aacaacaaaa
     2341 aatgagtaca gaataccaat catttattac cgaattaaaa gaaaaagctg atattaagac
     2401 tgtagctaag tattaataaa gtagatgctg ctcaaatcta caagaacatt agaagaa
//
LOCUS       DAOTFA010000063         2392 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate AMR_MDS_3115
            NODE_36714_length_2392_cov_10.363714, whole genome shotgun
            sequence.
ACCESSION   DAOTFA010000063 DAOTFA010000000
VERSION     DAOTFA010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868139
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (wastewater metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:36:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 536
            CDSs (total)                      :: 521
            Genes (coding)                    :: 520
            CDSs (with protein)               :: 520
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36714_length_2392_cov_10.363714"
                     /isolate="AMR_MDS_3115"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2375)
                     /gene="ppdK"
                     /locus_tag="P5014_02345"
     CDS             complement(<1..2375)
                     /gene="ppdK"
                     /locus_tag="P5014_02345"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013006994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HRY56983.1"
                     /translation="MQKYIYFFGNNNTEGSSDMKFILGGKGANLAEMSKIGIPVPPGF
                     TISTEVCRDYNSNNGTIPEGLESEVEDYLKKLEDATGKKFGDESNPLLVSVRSGAAAS
                     MPGMMDTILNLGLSETVVSSISQSTGNPRFAWDSYRRFIQMYGNVVENIEHEKFEHII
                     EEVKSSKGKKEDIEMTAEDWQEVVAEYKKLFQIEKDYEFPDDAKKQLWGAILAVIKSW
                     NNARAETYRRLNHIDESKVFGTAVNVQSMVFGNLGPESGTGVAFTRNPSTGENVSYGE
                     YLMNAQGEDVVAGIRTPKHLDSLKMENEAVYNQLMEIFSILEKHYRDMQDVEFTIENG
                     KLFILQTRNGKRTAPASVKIAVDLVSEGLISEKEALLRVEPDKLDQLLHPTLDQNEKS
                     KFKMIAKGLPASPGAGVGQVVFDAESAVEWKKQGKKVILVRLETSPEDIAGMNAAAGV
                     LTARGGMTSHAAVVARGMGKCCVSGCESISVSEADKKMHVGDLVVLEGDLITLDGSTG
                     EVFLGEVPTTDPELSGYFGTIMEWSDKYKKLGVRTNADTPKDAQVARDFGAKGIGLCR
                     TEHMFFETDRIFAVRQMIVSDTVEQRKSALEKIFPIQKNDFKELFQIMKDFPVKIRLL
                     DPPLHEFLPKTKEDIKELASEIGVSEEKLNEKIEYLHEFNPMMGHRGCRLGVTYPEIF
                     EMQVRAIAEAACEIIKEGSSVTPEIMIPLIGEFNELKWLKDRLVPVIEETIQKFGVEM
                     KYEIGTMIEIPRAALTADKIAEEADFFSFGTNDLTQMTYGFSRDDAGKFLG"
BASE COUNT          680 a          536 c          342 g          834 t
ORIGIN      
        1 tctcctaaaa attttcctgc gtcgtctctt gaaaaaccat aagtcatttg tgttaaatca
       61 ttggttccaa atgaaaagaa atcagcctcc tccgcaattt tatctgcagt taaagcagct
      121 ctgggaatct ctatcattgt tcctatttcg tatttcatct caaccccgaa tttctggatt
      181 gtttcctcaa ttacaggaac aagtcgatct ttcaaccatt tcaactcgtt aaattctcct
      241 ataagaggaa tcattatttc aggagtaact gaactacctt ctttaataat ctcacaggcg
      301 gcttctgcaa tagctcttac ctgcatttcg aatatttcag gatacgtaac accgagtcgg
      361 caaccacgat ggcccatcat tggattaaat tcatgtaaat attcaatttt ttcatttaat
      421 ttttcttctg aaactccaat ttcgcttgcc aattctttta tatcttcttt tgttttaggt
      481 aaaaactcat gaagaggtgg atctaaaagt cttatcttaa cgggaaagtc cttcattatc
      541 tgaaataatt ctttgaaatc atttttttgt attggaaata ttttttcaag agccgacttt
      601 ctttgttcaa cagtatctga aacaatcatt tgtctaactg caaatattcg atcggtctca
      661 aaaaacatat gctcggttct gcaaagacca attcctttgg caccaaaatc tctagctacc
      721 tgagcatctt ttggagtatc ggcattagtt cttactccaa gttttttata tttatcggac
      781 cattccataa tagttccaaa gtaccctgaa agctctggat cggttgtagg aacttcacct
      841 aaaaacactt cgccggttga accatcaagt gttattaaat caccttctaa aactactaaa
      901 tctccaacat gcatcttttt atcagcctca gaaactgaaa tggactcaca ccccgaaaca
      961 cagcattttc ccatacctct tgcaactaca gctgcatgag aagtcatacc accacgagca
     1021 gttaaaacac ctgcggctgc gttcattcct gcaatatctt ccggagatgt ttctaatctg
     1081 accaaaatta cttttttacc ttgttttttc cattcaactg ctgattcagc atcgaaaacc
     1141 acttgaccga cacctgcgcc aggagaagcg gggagccctt tagcgatcat tttaaatttt
     1201 gatttttcat tttgatcaag tgtcgggtgt aagagttgat ctaatttatc cggttctact
     1261 cttaataatg cctctttttc acttatcaga ccttcgctta ctaaatccac ggctatttta
     1321 actgaagctg gggctgtcct ttttccattt ctcgtctgca atataaaaag ctttccattt
     1381 tcaattgtaa actcaacatc ttgcatatct ctgtaatgtt tttcaagaat tgaaaatatc
     1441 tccatcaact ggttataaac agcttcgttt tccattttta aagaatccaa gtgcttggga
     1501 gttctaatac ctgctacaac atcctcaccc tgagcattca ttaaatactc accgtaagaa
     1561 acattttcac ctgtggaagg atttctcgta aaagcaactc ctgttccaga ttccggacct
     1621 agatttccaa aaaccataga ttgaacatta actgcggtcc caaatacttt agactcatcg
     1681 atgtggttta gtcttcggta ggtttcagct ctagcattat tccatgattt tataacagct
     1741 aaaattgctc cccataattg ctttttagca tcatcgggaa attcataatc tttttcaatt
     1801 tggaataatt ttttgtactc tgctacaacc tcctgccaat cttccgcagt catttcgata
     1861 tcttcttttt taccttttga agacttaacc tcctcaataa tgtgttcaaa tttctcatgt
     1921 tcgatatttt cgaccacatt tccgtacatc tgtataaatc ttctatacga atcccaagca
     1981 aatcttggat taccggtact ttgtgaaata cttgaaacaa ccgtctcgct taatcctaaa
     2041 tttaaaattg tatccatcat gccgggcata gaagccgcag ccccagatct tactgagact
     2101 aaaagagggt tactctcatc accaaatttt ttacctgtag catcttctaa tttttttaaa
     2161 taatcttcta cttctgattc taaaccttct ggaattgtac cgttattaga attgtagtct
     2221 ctgcatactt cggtagatat tgtgaaacca ggtggcacag gaattcctat tttactcatt
     2281 tcagcaaggt ttgcaccttt accccctaat ataaatttca tatccgagga accctctgta
     2341 ttattatttc caaaaaagta tatgtatttt tgcattggta tactttaaac tt
//
LOCUS       DAOTFA010000064         2390 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate AMR_MDS_3115
            NODE_36773_length_2390_cov_3.861242, whole genome shotgun sequence.
ACCESSION   DAOTFA010000064 DAOTFA010000000
VERSION     DAOTFA010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868139
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (wastewater metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2390)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:36:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 536
            CDSs (total)                      :: 521
            Genes (coding)                    :: 520
            CDSs (with protein)               :: 520
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36773_length_2390_cov_3.861242"
                     /isolate="AMR_MDS_3115"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="P5014_02350"
     CDS             <1..502
                     /locus_tag="P5014_02350"
                     /inference="COORDINATES: protein motif:HMM:NF017102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HRY56984.1"
                     /translation="KPDFNKMDSKYSYSGNIIASPGAGVPLWDQTIYDQMGGTDISKG
                     EWDILSYNYPTALFWCTWLPTKVYNQIYGDNYGSNYYAGGMLNLGARAMCEQTNLNFI
                     KDYSPQPGDIACFVTGEEWHHVGVVYSVDADSIQVVESNSGQIMNLYTESNGSYYPIS
                     YFKRVR"
     gene            503..1018
                     /locus_tag="P5014_02355"
     CDS             503..1018
                     /locus_tag="P5014_02355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56985.1"
                     /translation="MPYQFKIKNIKNKKLVAVLFISTVLIIILIFSSNRKKPGSDYKT
                     ITDNKTNVSKSSKDEQSEDSGVTKPPVPETGKTIIEQISNDYEFTKYWENIYREYPWY
                     KKLPIVNNDYKIYWVVQEKSFRIRLAVSENSPEEQKRDIINKALKDIEDLTGVSYENY
                     PYYVLYTENKN"
     gene            complement(1104..1544)
                     /locus_tag="P5014_02360"
     CDS             complement(1104..1544)
                     /locus_tag="P5014_02360"
                     /inference="COORDINATES: protein motif:HMM:NF039493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56986.1"
                     /translation="MDNYLKKIKHHKNLIFISVFLIQSSVLSFAEEGDPPSLTSLSSI
                     LSRIVGLFLAASAAALIIMVAYGIIKGSLAAGDPRGLEGAKSTWTYALYGFLIIVLSM
                     VILTLIRKVLGLSTGDERLGFKTFLDKILDSIEELVGIGQRSGS"
     gene            complement(1547..1861)
                     /locus_tag="P5014_02365"
     CDS             complement(1547..1861)
                     /locus_tag="P5014_02365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56987.1"
                     /translation="MFDLLNDSIAKSNIEVDNPGLGSGIGWIISTAVNTVTVIAFGVS
                     FVMLAYAFVQFITSTGDPKRLSVPKSAITWSVIGLILATLLQGIKLLILRTLGYDSGK
                     FF"
     gene            complement(1929..2255)
                     /locus_tag="P5014_02370"
     CDS             complement(1929..2255)
                     /locus_tag="P5014_02370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56988.1"
                     /translation="MIKAVLIQKVFAEVSFDDPDVYGGGIFQGAELDKVFEISANIVI
                     WVGLGLVLVFLALGFIKFITSQGDKVATEQAQKWVTYSIIGGVGLLAVFAIRGIIMQI
                     AGYKKE"
BASE COUNT          866 a          456 c          391 g          677 t
ORIGIN      
        1 taaacctgac tttaataaaa tggattcgaa atattcttat tcgggaaata taatagcatc
       61 gccaggtgcc ggggttccgt tatgggatca aacaatttat gatcaaatgg gaggtacaga
      121 cataagcaaa ggtgagtggg acatccttag ctataattac ccaacggctt tattttggtg
      181 tacgtggctt cctactaaag tatataatca gatatatgga gataattatg ggtctaatta
      241 ttacgcaggt ggtatgttaa atttaggtgc tagagcaatg tgtgagcaga caaacctaaa
      301 ctttataaaa gattactctc cccagcctgg tgatattgcc tgttttgtaa ctggagaaga
      361 gtggcatcac gtcggagttg tgtactcagt ggatgcagat tcaatacagg ttgttgagag
      421 taactccggg cagattatga atctctacac agaatcaaat ggatcctact accccataag
      481 ttattttaaa agggtcagat aaatgccata tcagtttaaa attaagaata taaaaaataa
      541 aaagttagtg gccgttttgt ttatatcaac agtattaata atcatattaa tattttccag
      601 caacagaaaa aaaccaggct ctgattataa aacaatcact gataataaaa ccaatgtttc
      661 taaatcctca aaagatgaac aaagcgaaga cagcggtgtt acgaaacccc ctgttccaga
      721 aacagggaaa acaattattg aacaaattag taacgattat gaatttacaa aatactggga
      781 aaacatatac agagaatatc cttggtacaa aaaacttcct atcgtaaata acgattataa
      841 aatatattgg gttgtacaag aaaaatcttt cagaataagg ttagcggtga gtgaaaacag
      901 ccccgaagaa caaaagagag acattataaa caaggctctt aaagatatcg aagatctaac
      961 aggagtatcg tacgaaaact acccttatta cgttctttat acggaaaata aaaactaaag
     1021 tttaatttaa agtgattttt ttataaaaag ttttacttcg gaatcggagt tacaggcaaa
     1081 taaccctcag acgcaccctg cggtcacgaa ccacttctct gtcctatacc aacaagctcc
     1141 tcaatcgaat caagaatttt atccaagaat gttttaaaac ctaatctctc atcacctgtt
     1201 gataatccaa gtacttttct aataagcgta aggattacca tagaaagtac gatgattaaa
     1261 aaaccataaa gagcataggt ccacgtactt tttgccccct ccaatcctct cggatcaccc
     1321 gcagctaaag aaccttttat tattccgtaa gctaccatga ttattaaagc agcggcagag
     1381 gcggctaaaa acaaacctac aatccttgac agtatactac taagactagt aagacttggg
     1441 ggatctcctt cttctgcgaa agagagcact gagctttgaa tcaaaaacac agatatgaag
     1501 ataaggtttt tatgatgttt tattttttta agataattat ccatttttaa aagaacttac
     1561 cagagtcgta tccaagcgtt ctaagaatca acaatttaat accctgcaaa agagtcgcaa
     1621 gtattaaacc tatgactgac caagtaatag cacttttagg aacactcaat ctcttagggt
     1681 cccctgtact agtgataaat tgaacaaagg cgtatgccag cataacaaag gagaccccaa
     1741 aagcgattac tgtaaccgta ttaacagcgg tggatataat ccacccaata cctgatccta
     1801 aaccagggtt atcaacctca atatttgatt tagctataga gtcattaagt aagtcaaaca
     1861 tatacgaaaa aattcctaaa tgaaattaca ccatcattta ttgtgctcat aaactctttt
     1921 caataaaact attccttttt ataacccgca atttgcatta ttatgcctcg aatagcaaaa
     1981 acggccaaaa gaccgactcc tccgattatt gagtacgtta cccatttttg ggcttgctct
     2041 gttgcaacct tatcaccttg tgaggtaata aattttataa aaccaagtgc aagaaaaact
     2101 aaaacaagac ctaaaccaac ccagataaca atgtttgcag atatttcaaa tactttatct
     2161 aactccgcac cttgaaatat ccccccacca taaacgtcag ggtcatcaaa agaaacctct
     2221 gcaaaaactt tttgaatcaa cacagctttt atcataactt aaaaattttc cgtaaaagtg
     2281 actaaatacc ttctccaatt atttggttga ttataacccg aagaatagtt atcgacaaca
     2341 atatgaaaat tatacctatt atagcggtgg ttaattgttc ttgagctgcc
//
LOCUS       DAOTFA010000065         2379 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate AMR_MDS_3115
            NODE_37034_length_2379_cov_6.519793, whole genome shotgun sequence.
ACCESSION   DAOTFA010000065 DAOTFA010000000
VERSION     DAOTFA010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868139
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (wastewater metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:36:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 536
            CDSs (total)                      :: 521
            Genes (coding)                    :: 520
            CDSs (with protein)               :: 520
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37034_length_2379_cov_6.519793"
                     /isolate="AMR_MDS_3115"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..305)
                     /locus_tag="P5014_02375"
     CDS             complement(<1..305)
                     /locus_tag="P5014_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56989.1"
                     /translation="MNKYALLILIIVPVVVFILTINYKDKTMQGGNPYDNSKDSEVIL
                     TDKTNVKPPTTKLDIIKTYKVKFKTTEGDIVIKVNALDTTMASTNFVYLSRIGFYDGT
                     "
     gene            complement(302..1246)
                     /locus_tag="P5014_02380"
     CDS             complement(302..1246)
                     /locus_tag="P5014_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009185246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRY56990.1"
                     /translation="MKNIIVTGGAGFLGSHLCEYLIKKGEKVICIDNLFTGNLKNLED
                     IKDHPNFEFIQHDIIDPIFFDGRKIDQIYNLACPASPIQYQINPVRTVKANTIGVINV
                     LGIAKKHKARIFQASTSEVYGDPLEHPQTESYRGNVNTIGERACYDEGKRVAETLFFD
                     YHREHGVEIRVARIFNTYGPRMYENDGRVVSNFIVQALNDDPITIYGTGEQTRSFCYV
                     DDLIEAIYRIMNTENITGPINIGNPGEFTMLELADKILKITDSKSSIIFKDLPNDDPK
                     KRKPDITLAKDLLDWEPKVTIDEGLVKTVGYFKKVLNK"
     gene            complement(1250..2083)
                     /locus_tag="P5014_02385"
     CDS             complement(1250..2083)
                     /locus_tag="P5014_02385"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRY56991.1"
                     /translation="MVKNTTNTRKKVGLALSGGGWRGMAHIGVLKVLEKNNIPIDFIA
                     GTSAGALMGGLYSYYGNIDQLEEFVLKFGYKDLFKMITDPRLKTGIIKGQKIIKYINE
                     KTNYVNIEDLKIPFSAVASDLVSGKPYYFKKGNLAEAIRTSISIPLLFQPTQKDGMIL
                     IDGGATENVPVNCLKEMGAEYIIASNVNSGIFPLGPGMIKNAGNIALVSTRTMLNTLS
                     DILTSQADFAIESKIPIEKMKIGVGYFLEFIKEKEVIAIGERAAESSIKELKKYLSDK
                     I"
     gene            2151..>2379
                     /locus_tag="P5014_02390"
     CDS             2151..>2379
                     /locus_tag="P5014_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56992.1"
                     /translation="MYISIDLGRSTTRIASTLNLKSVLKIVKFKTESDITFQRNNMFT
                     AFDEVAGGKDIDAISFGFPGITDKLKKVFEKS"
BASE COUNT          703 a          431 c          357 g          888 t
ORIGIN      
        1 gttccgtcat aaaaaccaat tctggataaa tatacaaagt tcgtagaagc cattgttgta
       61 tcgagtgcgt taaccttaat aacaatatcc ccctcggtag ttttaaattt aactttgtaa
      121 gtcttaatta tatccagttt tgttgtaggt ggttttacat tagttttgtc agtaagtatt
      181 acttcactgt ctttggaatt gtcataagga tttcctccct gcattgtttt atctttatag
      241 ttgatagtta atataaatac tacaacagga acaattatta atattaatag tgcgtattta
      301 ttcatttatt taataccttc ttaaaataac ctactgtttt aacaagacct tcatcgatag
      361 taacttttgg ttcccaatca agcaagtctt ttgccagtgt tatatcgggc tttctttttt
      421 taggatcatc gttgggaaga tctttaaaaa ttatactaga ttttgaatct gtaattttaa
      481 gtattttatc tgcaagttct agcatagtaa actcgcctgg attaccaata ttaatcggac
      541 ctgtgatatt ttccgtattc ataattctat agatagcttc aataaggtcg tctacataac
      601 aaaaagatcg tgtttgttct ccagtgccgt agatagtaat gggatcgtca tttagtgcct
      661 ggacgataaa attagataca actctaccgt cattctcgta cattcttggg ccatatgtat
      721 taaatattct cgctactctt atctcgactc cgtgttccct gtggtaatca aaaaacaggg
      781 tttcggcgac ccttttcccc tcatcatagc aagctctctc accaatagta tttacgttcc
      841 ccctgtagga ctctgtttga ggatgctcta aagggtcgcc gtaaacctct gatgtagaag
      901 cttgaaaaat tcttgctttg tgtttttttg caattcccaa aacgtttata actccaattg
      961 tattagcctt tacagttcta actggattta tttggtattg gattggggat gccggacaag
     1021 ctaaattata tatttgatct attttcctgc catcaaagaa aattggatca ataatatcgt
     1081 gttgtataaa ctcgaaattt ggatgatctt taatatcttc taaatttttt aaattacccg
     1141 taaagaggtt atcaatgcag attactttct cccctttttt tattaaatac tcgcataagt
     1201 gactacctaa aaaaccagct cctcctgtta cgataatatt tttcataagc tatattttat
     1261 cagataaata tttttttaat tctttgatac tactttctgc ggccctctcg cctatggcga
     1321 ttacttcttt ttcctttata aattctaaaa agtaacctac accaatcttc atcttttcta
     1381 ttggaatttt agattctatg gcaaagtcgg cttgtgatgt taatatgtca gacaaggtgt
     1441 ttaacattgt tcttgttgat actaaggcaa tattcccagc attttttatc attcccggcc
     1501 ccaacggaaa tattccggaa tttacgttac tggcaataat gtattcggca cccatttcct
     1561 ttaagcaatt taccggtaca ttctctgtag ctcccccatc aattaaaatc atcccgtctt
     1621 tttgtgttgg ttgaaaaagt aacgggattg aaatactagt tcttattgct tctgcgaggt
     1681 ttcctttttt aaagtagtac ggtttgccgg atactaaatc tgaagcaact gcagaaaatg
     1741 gtatttttaa atcctcaata ttaacgtaat ttgttttttc atttatgtat ttgataattt
     1801 tttgaccttt aataatcccc gttttaagtc gtgggtcagt tatcatttta aaaagatctt
     1861 tgtatccaaa tttgaggaca aactcttcta attgatcgat attgccgtaa taactatata
     1921 aaccacccat taaagctcca gcacttgtcc cagcaataaa atctattggg atattatttt
     1981 tttcaagtac tttcaataca cctatatgag ccatacctct ccatccccca ccacttagtg
     2041 ctaacccaac tttttttctt gtatttgtag tattttttac catttaaaat aatatatcac
     2101 acgagcgata cttttttact aaagtatttc ttgctactat taagatacta atgtatatct
     2161 caattgacct cggaagatct acaacaagaa tagcctcaac tcttaatcta aagagtgttt
     2221 taaaaattgt taaattcaaa acagaatcag atattacttt tcaaagaaat aatatgttta
     2281 ctgcttttga tgaggtggca ggaggtaaag atatagatgc tatttcattt ggttttccgg
     2341 gaataacgga taaattaaaa aaagtatttg aaaagagtg
//
LOCUS       DAOTFA010000066         2216 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate AMR_MDS_3115
            NODE_41518_length_2216_cov_5.160111, whole genome shotgun sequence.
ACCESSION   DAOTFA010000066 DAOTFA010000000
VERSION     DAOTFA010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868139
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (wastewater metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2216)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2216)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:36:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 536
            CDSs (total)                      :: 521
            Genes (coding)                    :: 520
            CDSs (with protein)               :: 520
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2216
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41518_length_2216_cov_5.160111"
                     /isolate="AMR_MDS_3115"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            27..350
                     /locus_tag="P5014_02395"
                     /pseudo
     CDS             27..350
                     /locus_tag="P5014_02395"
                     /inference="COORDINATES: protein motif:HMM:NF016119.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
     gene            complement(358..852)
                     /locus_tag="P5014_02400"
     CDS             complement(358..852)
                     /locus_tag="P5014_02400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56993.1"
                     /translation="MDQNNNAYSIKTQSTKGLSTFVLTLSISLIVFSAIYYFMTTKAS
                     TSEYDAFNVSVIDEKKSEESSSKPVTQKNSDEDSSVFGKLASASPDSNSGQVLAGTDV
                     IETEVQAEVPQTTQSNLETGVTSITFGLVSALALFVSGMVFIHKGPRKAALSIFEKNI
                     TGRI"
     gene            complement(839..1030)
                     /locus_tag="P5014_02405"
     CDS             complement(839..1030)
                     /locus_tag="P5014_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56994.1"
                     /translation="MKLFFTTLGIFITVLLVWLVIGMKKIDRNTFLPSAVLDKAVPLN
                     AIIDIEFINNLKNPANGSK"
     gene            complement(1027..1581)
                     /locus_tag="P5014_02410"
     CDS             complement(1027..1581)
                     /locus_tag="P5014_02410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56995.1"
                     /translation="MKKIDEFLNLIRRILSNESISIKIYIVWGLITLLMFGFMGFLPV
                     SKIFLSNVALLDDLYKNNLNLEKKIDELKVAKEKVELVGEDINILDKFLPDEFQAQNY
                     LVDMSIIFGQSGYSLDSISFTNKDANEVNMSLRISGKGNLKDLVSKLEESKKLNEIQS
                     IRYSVGSREDSLNMTIKSFIMENK"
     gene            complement(1581..>2216)
                     /locus_tag="P5014_02415"
     CDS             complement(1581..>2216)
                     /locus_tag="P5014_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56996.1"
                     /translation="MLEKLLPSIFKKKIRSNLNQENQQRNLSNDNKESVSLDSNFNLI
                     SKSQKLYIAPKFASEFILIIRKFIVGISVIFGVLLILNFTLSFMIQYQKGWQSKLVEE
                     IQTYSDIEEKAKRISDKTVMYKKFLSSRFLISDKVDYVIDNIGSEIELSNLDISQQGF
                     SISISGKDAIDFTNLIVRYLEDGLVSEIVINSASLDKSENIYKVMLRGNFK"
BASE COUNT          735 a          393 c          322 g          766 t
ORIGIN      
        1 cccgatgatt tcaaggacat tacaaagggt ctgcgctacc tggcgaggtt ggagactctt
       61 ttatttatgg acactcggta ttgccttggt tttttaatcc taaaaattac aaaacaattt
      121 tctcaactct cgggaattta gaaaccggtg atagatttac aatagaatac aatcaaagaa
      181 aaattaacta caaagttgag gataaagtgg ttctttcacc taaagaagta gatcccttgg
      241 aaaactggaa acctatgtat ctcaatcaac ccacagtaag tttaatgaca tgctggcctg
      301 cagggactaa aacaaataga ttaattataa gagctgttca ggaaaattaa attcggatta
      361 aattcttcca gtaatatttt tttcaaaaat acttaaagca gcttttctcg gacccttgtg
      421 tataaaaacc attcccgata cgaacaaagc gagagctgat actaaaccaa atgtgatgct
      481 tgttacacct gtctcaagat tgctttgagt agtctgagga acttcggcct gtacttcggt
      541 ctctataaca tcggttcctg ctaaaacctg tccggaatta gaatctggag aggcacttgc
      601 taactttccg aaaacagaac tatcctcatc cgaatttttc tgagtaactg gttttgaaga
      661 ggattcttct gacttttttt cgtcaataac actaacatta aaagcgtcgt attcggaagt
      721 agatgccttt gttgtcataa aataataaat tgcactgaaa acaataagcg aaattgaaag
      781 cgtgaggaca aaagtgctta aaccttttgt tgactgtgtt tttatgctgt atgcgttatt
      841 attttgatcc attggcagga tttttcaaat tattaataaa ttctatatca atgatagcat
      901 tcaaaggtac agctttgtca agtactgctg aaggaagaaa ggtgtttctg tcaatttttt
      961 tcataccaat aactaaccag acaagtaata ctgtaataaa tattccaagt gtagtaaaaa
     1021 ataatttcat ttattctcca taataaaaga cttgatagtc atattcaaac tatcttctct
     1081 gctacccacg ctgtatctaa tgctttgaat ctcgtttaat ttttttgatt cctctaactt
     1141 agataccaaa tcctttaaat ttccttttcc tgatatacgg agactcatat taacctcgtt
     1201 agcatcttta tttgtaaaac taatcgaatc aagagaatac cctgactgac caaatattat
     1261 agacatgtcc actaagtaat tctgagcttg aaactcatcg ggaagaaact tatctaaaat
     1321 attgatgtcc tctccaacta attctacttt ttcttttgcc acctttaact catcaatttt
     1381 cttttctaag ttaagattgt ttttatataa atcatccaga agggctacgt ttgataagaa
     1441 aatttttgat actggtaaaa atcccatgaa gccaaacatt aataacgtta ttaaacccca
     1501 tacaatataa attttaatag agatggattc gtttgaaaga attctcctta ttaaatttaa
     1561 aaattcatct atttttttca ttatttaaag ttacctctta acataacctt atatatattc
     1621 tccgacttat ctaaacttgc tgaattaata acaatctctg agaccaaacc atcttccaaa
     1681 tacctaacaa taagatttgt aaaatctata gcgtcttttc ctgatataga tatggaaaaa
     1741 ccttgctggc taatatcaag gttactaagc tcaatttcgg aacctatgtt atcaatcacg
     1801 taatctacct tgtcagatat taaaaaccta cttgataaaa actttttata cataactgtt
     1861 ttatcactaa ttctctttgc tttttcttca atatctgaat aggtttgtat ttcctctacc
     1921 aatttactct gccacccttt ttgatattga atcatgaaag aaagggtaaa attaaggatt
     1981 aatagaacac caaaaatcac acttataccg acaatgaatt ttctaattat gagtataaat
     2041 tcggacgcaa atttaggggc aatatataac ttctgtgatt tagaaattag attaaagttg
     2101 gaatctaagg aaacactttc cttgttatcg ttacttagat ttctttgttg attttcttgg
     2161 tttagattcg accttatctt ctttttgaag atcgagggca gcaatttttc tagcat
//
LOCUS       DAOTFA010000067         2193 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate AMR_MDS_3115
            NODE_42237_length_2193_cov_6.003274, whole genome shotgun sequence.
ACCESSION   DAOTFA010000067 DAOTFA010000000
VERSION     DAOTFA010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868139
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (wastewater metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:36:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 536
            CDSs (total)                      :: 521
            Genes (coding)                    :: 520
            CDSs (with protein)               :: 520
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42237_length_2193_cov_6.003274"
                     /isolate="AMR_MDS_3115"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(90..1208)
                     /gene="ychF"
                     /locus_tag="P5014_02420"
     CDS             complement(90..1208)
                     /gene="ychF"
                     /locus_tag="P5014_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016838918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HRY56997.1"
                     /translation="MSSLSIGIVGLPNVGKSTLFNALLKRQAALAANYPFATIEPNVG
                     VVDVPDHRLDELCKIVITDYGRGSEGREIPEKVTPAVVKFYDIAGLVKGASKGEGLGN
                     AFLGHIREVNAIAHVVRSFEDENVVRAGSVDPISDKETVNTELILADIQSLENTIGKL
                     EKDIRGKPEKEAKIKYETAKKILEKLNQGIFAKDIGLEEEELEASKDLNLLTQKPQIY
                     VLNVSEDYLSNKNDQNIDENIKDSIVICAKLESELADFDKNERENYLKELGIEYSGLD
                     RLIKKGYEMLGLETFLTAGPKEVRAWTIKKGSKAPQAAGEIHTDFERGFISAEVINFN
                     TLLEIGSMKKAKEKGLIRLEGKNYVMQDGDVVLFRFSV"
     gene            complement(1270..>2193)
                     /locus_tag="P5014_02425"
     CDS             complement(1270..>2193)
                     /locus_tag="P5014_02425"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HRY56998.1"
                     /translation="ILEKNDGIRQFMSWNGAVISDSGGFQVMSLAKKGDSKGLITDEG
                     VTFKDSNGRKTKLTPEESIRFQMLLKPDMVVVLDDFTEPDCTYEKAKETVERTILWAK
                     KSKEEFLKICKKEKIKKENRPYLLGVVQGGEFLDLREYCTKELVKIGFDGLGYGGWPM
                     KDNGDFNFDVPRVIAENSPENYLLYGLGVGKPEDIIECFYLGFYIYDCVLPTRDARHS
                     RLYVYNANIINDINIRDDNFYSFFDPSKQKYYHDAEPVSTACDCLLCKKYSRSYLHHL
                     FKNDETTALRLATIHNLRFYSLLMERLGEEV"
BASE COUNT          659 a          443 c          296 g          795 t
ORIGIN      
        1 tttgaataga cattaagtaa agacttggaa atgttatcaa aaatgggaaa aaaggcaagt
       61 agtatgataa ccaagtgagt aagatttttt tatacactaa acctaaacag aaccacatct
      121 ccgtcctgca taacataatt cttaccctca agtctaatca aacccttttc tttagccttt
      181 ttcatgctcc ctatttctaa tagagtatta aagtttatga cctcagcaga tataaagcct
      241 ctttcaaaat ctgtatgaat ttctccagcg gcttggggag cttttgaacc ttttttaata
      301 gtccaagctc tgacttcctt tggacccgca gtcagaaatg tttcaagacc taacatttca
      361 tatccttttt taattaatct gtctaaacca gaatattcaa ttccaagttc ttttaagtaa
      421 ttttcgcgtt catttttatc aaaatctgct agttccgact ctaattttgc acatataaca
      481 atgctgtcct ttatattttc gtcgatattt tgatcatttt tattactcaa ataatcctca
      541 ctaacattta aaacataaat ttggggtttt tgagttaata aatttaaatc tttcgatgct
      601 tccaactcct cttcttctaa tccgatgtct tttgcaaaaa ttccttgatt tagtttttca
      661 agaatttttt tagcggtttc atattttatt tttgcttctt tttcaggttt ccctctaata
      721 tctttttcca acttcccaat agtgttttcc aatgattgaa tatcagcaag aataagctcg
      781 gtgttaaccg tttctttgtc agatatagga tctacagaac ccgctctgac aacattttca
      841 tcctcaaagc ttctaactac gtgggctatc gcattcactt ctcttatatg tcctaaaaaa
      901 gcatttccga gtccctcacc ttttgacgct ccttttacta aacctgctat atcgtaaaat
      961 ttaactactg ccggtgttac tttttcaggt atttctcttc cttcagaacc tcttccgtag
     1021 tcagttatta ctattttgca taattcatct aatctgtggt caggaacatc aacaacacct
     1081 acgtttggtt ctattgtagc aaacggatag tttgccgcta atgcggcttg acgttttaat
     1141 agtgcattaa aaagggtgga tttcccgacg ttaggaaggc ccacaattcc tatcgatagt
     1201 gatgacataa gagtatttta acagcgagaa tgaaaaatgg aaatgaaata tatcttgata
     1261 atattttaac tatacttctt ctcccaacct ctccatcaac agactataaa atctcaaatt
     1321 atgaatagtg gctagtctca aagctgtagt ctcatcgttt ttaaataggt ggtgtaaata
     1381 ggatctggaa tattttttgc ataacaaaca atcgcaagct gtactcacag gttctgcatc
     1441 atgataatat ttttgtttgc ttggatcaaa aaatgagtaa aaattgtcat cacgaatatt
     1501 tatgtcgtta attatattag cattatatac atacaatctg ctatgacgag catctctagt
     1561 tggtagaacg caatcgtaaa tataaaaacc taaataaaag cattcgatga tatcttcagg
     1621 ttttcctact ccaagcccgt ataaaagata gttttcagga gaattttctg caattactcg
     1681 aggaacatca aaattaaaat ctccgttatc tttcattggc cagccaccgt aacctaaacc
     1741 gtcgaaacca atttttacaa gttcttttgt acaatattct cgtaaatcta aaaactctcc
     1801 gccctgcact actccaagta aataaggtct gttttctttt ttaatttttt ctttcttgca
     1861 aatttttaaa aactcctctt tagatttttt agcccaaaga atagttcgtt caacagtttc
     1921 ttttgctttt tcataagtac aatctggttc tgtaaaatca tctaaaacca caaccatatc
     1981 tggctttaaa agcatttgaa atctgataga ttcttctgga gtaagtttcg tttttctacc
     2041 attagaatct ttaaaggtta caccttcgtc agttatcaaa cccttgctat ctcctttttt
     2101 tgctaaactc ataacctgaa aacctcctga atcagatatc accgccccgt tccatgacat
     2161 aaattgtctt attccatcat ttttttcaag aat
//
LOCUS       DAOTFA010000068         2185 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate AMR_MDS_3115
            NODE_42493_length_2185_cov_7.909859, whole genome shotgun sequence.
ACCESSION   DAOTFA010000068 DAOTFA010000000
VERSION     DAOTFA010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868139
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (wastewater metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:36:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 536
            CDSs (total)                      :: 521
            Genes (coding)                    :: 520
            CDSs (with protein)               :: 520
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_42493_length_2185_cov_7.909859"
                     /isolate="AMR_MDS_3115"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(54..272)
                     /locus_tag="P5014_02430"
     CDS             complement(54..272)
                     /locus_tag="P5014_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY56999.1"
                     /translation="MNGTQNLNIDSKTLEQCLKAISNFGATTYQNICTGESTVVVWGG
                     ADWFGGVVITIFLLAFIVWFLKEMFTSY"
     gene            complement(479..856)
                     /locus_tag="P5014_02435"
     CDS             complement(479..856)
                     /locus_tag="P5014_02435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY57000.1"
                     /translation="MTSQECEHVKDLFGYKLWKVMKWAEAKGKMDVFQACYERAKELN
                     HRDYGDWNEAKSQFMTMNFPTVKKDDKYFYQTTRLLGYLVSFSQEMDDEMTIKSKSMA
                     IYNQKTNDCIYRMLVANKEVLFQ"
     gene            complement(828..1568)
                     /locus_tag="P5014_02440"
     CDS             complement(828..1568)
                     /locus_tag="P5014_02440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY57001.1"
                     /translation="MKTRIFWTKTWKDDWFQSLSEQGQKLFLYLITNDLIGFSGCYQI
                     SDYSLKTEARIKDTDKIKKELYPKVKFFKDWVYIVNSERYNSFRGSTEIAKERELKLI
                     PENIKESLFKGEGEGGLPHSPPLGTPHTNTNTNNNINKYRNISDIKEDEIKAVAEKYK
                     VTIKYVERVLDRLANDIEIKGKDKYANKLAALRNWVSKQIEWDEKDNKKVGLKDLEPN
                     QILDIRENPERLIFYQRRGYDISRMRAR"
     gene            complement(1565..2131)
                     /locus_tag="P5014_02445"
     CDS             complement(1565..2131)
                     /locus_tag="P5014_02445"
                     /inference="COORDINATES: protein motif:HMM:NF013964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HRY57002.1"
                     /translation="MKKLERTQIGDFLYKNKGSTGVARQGFYGLTRDVFELALTEYLK
                     TGIDHLSEYDELTYLGWDLSGDEYSMEQISLLNRAMDHQTFWEIRALYTPEEIEEMLE
                     HSMVSLSLINYRKSYSHRRKQGFTQKIKNDCKKRDEYKCKVCHSKEGLVIHHIKEVVK
                     GGENELDNLITLCRNCHKKIHSKKGARK"
BASE COUNT          602 a          438 c          361 g          784 t
ORIGIN      
        1 ggatttaagg ccttttcgta aaaacttcca tctttttgtc ataaaatttt aaattaataa
       61 ctagtaaaca tctcttttag aaaccaaaca ataaaggcaa gtaaaaatat cgtgatgaca
      121 acgcctccga accaatctgc tccaccccaa acaacaaccg tgctttcacc ggtgcagata
      181 ttttgatagg tggtagcgcc aaaattacta attgctttaa gacattgctc taaggtttta
      241 gagtcaatat taagattttg tgttccgttc atattttttt aaatttataa ccccacccga
      301 gaagaagagg gaggttgggg tgtcgctggc gagaacggca ggcaacttcc cccatctttt
      361 cgagtggtga gtcttgagac tcggagaaga gggatgggca gaacaacgtg tataaatgct
      421 ccctctccct tctctccaag gcccaagagc cttggcaata acgtgtaacc tgctttgctc
      481 attgaaaaag aacctcctta ttagccacta acattcgata aatacagtca ttggtctttt
      541 gattgtagat agccattgat ttagatttga tggtcatttc atcgtccatt tcctgagaaa
      601 aactaactaa atagcctaaa agtctggtag tttggtagaa gtatttatcg tcttttttga
      661 ccgtagggaa gttcatggtc ataaactgac tcttggcttc attccagtct ccataatctc
      721 ggtgattgag ttctttggct ctttcgtagc aggcttggaa aacatccatc ttgcctttgg
      781 cttcagccca cttcataact ttccagagct tgtaaccgaa taaatcttta acgtgctcgc
      841 attcttgaga tgtcataacc tctcctttga taaaaaatta atcgttcagg gttctcacga
      901 atatctagga tttgattagg ttcaaggtct tttaaaccaa cctttttatt gtctttctcg
      961 tcccattcaa tctgtttact cacccagttc cttaaagcgg ctagtttatt agcgtattta
     1021 tcttttccct ttatttcaat gtcgttagct aagcgatcta aaactctttc aacgtatttg
     1081 atggtaactt tgtatttctc agcaactgct tttatttcat cttccttaat atcacttata
     1141 tttctatatt tatttatatt attattagta ttagtgttag tatggggggt acctaagggt
     1201 ggactatggg gtaacccccc ctctccttct cccttaaata aggactcttt tatattctct
     1261 ggaattagtt ttagctctct ttctttagca atttcagtac ttcctctaaa agagttatag
     1321 cgttcagagt tcacaatata gacccaatct ttaaagaatt tgaccttagg atagagttct
     1381 tttttaatct tgtctgtgtc ttttattcga gcttctgtct taagagaata gtcgctgatc
     1441 tgataacagc cggagaaacc gataaggtca ttggtgatga gatataaaaa tagcttttgt
     1501 ccttgttctg agagagactg aaaccaatca tctttccatg ttttagtcca gaagattcta
     1561 gttttcactt tctcgctcct tttttggaat gaattttttt atggcaattt cggcacaaag
     1621 ttattaaatt atctaattcg ttttctcccc ctttaacgac ctctttaatg tgatggataa
     1681 ctaaaccttc ctttgaatga caaaccttgc acttgtattc atctcttttt ttacaatcat
     1741 tttttatttt ttgggtaaat ccctgctttc gtctatggct atatgatttt ctgtaattga
     1801 ttaacgaaag agacaccatt gaatgttcaa gcatttcttc tatttcttcc ggagtataaa
     1861 gtgctctaat ttcccaaaac gtttggtgat ccattgctcg attaagcaat gaaatctgtt
     1921 ccattgaata ctcatcacca cttaaatccc accctaagta agtcaattca tcgtattcag
     1981 aaagatggtc tatcccggtt ttaagatatt cagttaaggc aagctcaaaa acatctcttg
     2041 ttaaaccata aaatccttgt ctagctacac cagtagatcc cttgttttta taaagaaaat
     2101 ctccaatttg agtacgctct aattttttca cttatggctc ctctatggtt agtgaataaa
     2161 taaattagtc ttattgacta aggtg
//
LOCUS       DAOTFA010000069         2115 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate AMR_MDS_3115
            NODE_44847_length_2115_cov_8.844175, whole genome shotgun sequence.
ACCESSION   DAOTFA010000069 DAOTFA010000000
VERSION     DAOTFA010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868139
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (wastewater metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:36:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 536
            CDSs (total)                      :: 521
            Genes (coding)                    :: 520
            CDSs (with protein)               :: 520
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44847_length_2115_cov_8.844175"
                     /isolate="AMR_MDS_3115"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(41..1141)
                     /locus_tag="P5014_02450"
     CDS             complement(41..1141)
                     /locus_tag="P5014_02450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY57003.1"
                     /translation="MKLEASHYDTVEWNPAPIEPGTFIKKPQKTYKCKLGKQKEIVLK
                     SPCRIDVGLIDYSALKFTDQNDYKAGEMSFACNVYTYVKVKLLDRPEIIIDSDRPVMV
                     KHYALLVKKVTDYKGGFEIKTIAPPYHHVGFGSSAIMAETTALAINKLLGNPLTSREL
                     RNLIAYNFVEESDNDKTKLFPGASTGGSFNTITNGGFVITSSECEKIFQEKIPDDMFF
                     VVGIPNVGVKGPEASETDVNCMGWERHNERINAAKSCLWILTEIMPYWVKGDYLKVGE
                     AFYNYTFFGGKGMQMLFYRCDQHGILFEQKEAGLEGGWMTSAGPGLVVFTQSQNKVKI
                     AQKIFKRRGCKQIVTVKGDNEGTIEVERLPKK"
     gene            complement(1142..1714)
                     /locus_tag="P5014_02455"
     CDS             complement(1142..1714)
                     /locus_tag="P5014_02455"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF024635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HRY57004.1"
                     /translation="MLNKLKSIKQNFVQNKPTLITLVGESGAGKSTLCKFIDLPDIWY
                     SSSGTIVKRLNQQGIPISHDSIHKFANQAYSENPEWQVPDILADMIEKGTLILDGPRR
                     IKEVEALRKKNINMVVIRITADQEERFRRLQLRDGVDRAAFERILRDESQETELGQLL
                     LMSDVIMENDGSLQDIQIRAQEIRNFINGK"
     gene            complement(1714..2052)
                     /locus_tag="P5014_02460"
     CDS             complement(1714..2052)
                     /locus_tag="P5014_02460"
                     /inference="COORDINATES: protein motif:HMM:NF015758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HRY57005.1"
                     /translation="MNISDIQKWERDFSLRKGIAKDEEKSIKIATLKLSEEVGEVCKA
                     ILEKKWEEVPAEVTDVIVFACKVANIAEDFHGVEKLEEVMKKKLEYSETRTYNPEINK
                     MDKPINKDFK"
BASE COUNT          630 a          431 c          267 g          787 t
ORIGIN      
        1 aaatcggtta cggtttcttc gctttaaaaa tagaaaaaaa ttatttcttt ggaagccttt
       61 cgacttcaat cgttccttca ttatctccct taacagttac tatttgttta catcctcgtc
      121 tcttaaatat tttttgagct attttaactt tgttctggct ttgagtaaac actactagtc
      181 caggacccgc agacgtcatc caaccacctt caaggccagc ttctttttgc tcaaaaagaa
      241 tgccgtgttg atcacaccta taaaacaaca tttgcattcc ctttcctcca aaaaaagtat
      301 agttgtaaaa agcttcgccc acttttaaat aatcaccctt aacccaatag ggcataatct
      361 ctgttaatat ccatagacat gattttgccg cattgattct ctcattgtgt ctttcccaac
      421 ccatacaatt aacgtctgtt tcagatgctt caggcccctt tactcctaca ttgggaattc
      481 caacaacaaa gaacatatca tctggaattt tttcttgaaa tattttttca cattctgaag
      541 aagtgattac aaaaccacca tttgtaatag tgttaaatga gcctcccgta gaagcaccag
      601 ggaagagttt cgttttatca ttatcacttt cttcaacaaa gttataagca ataagatttc
      661 tcagctctcg actggttaat ggatttccca gtaatttatt aatggctaaa gctgttgttt
      721 ctgccataat tgcactagaa ccaaagccaa catggtgata aggaggagct attgttttaa
      781 tttcaaaacc acctttataa tcagtgacct tttttaccaa caaagcataa tgcttaacca
      841 ttaccggccg atcactatca ataataattt ctggtctatc taataatttt accttaacat
      901 aggtataaac attgcatgca aaagacattt ctcctgcttt ataatcattt tgatctgtaa
      961 actttaatgc tgaataatca atcaatccca catcaatacg acagggtgat tttaaaacaa
     1021 tttctttttg ttttcccagt ttacatttat aagttttttg aggtttttta ataaaagtcc
     1081 ctggttcaat tggagctgga ttccattcga cagtgtcata atgacttgct tctaatttca
     1141 tttatttacc attaataaaa tttctaattt cttgagctct tatttgaata tcctgcaatg
     1201 aaccatcatt ttccataata acatctgaca ttaatagcaa ctgtcctaat tctgtctcct
     1261 ggctttcatc tcgaagaatc ctttcaaaag cagctctatc tacaccatca cgtaattgta
     1321 atcttctaaa gcgttcttct tgatctgctg ttattctaat taccaccatg ttaatatttt
     1381 tttttcttaa tgcctcgact tcttttattc gacgaggtcc atccaaaatt aatgtccctt
     1441 tttcaatcat atccgcaagt atatctggga cttgccactc agggttttca gaataagctt
     1501 gattggcaaa tttatgaata ctgtcgtgag agattggaat tccttgctga ttaagtcttt
     1561 tcacgatggt tccacttgaa ctataccaaa tatctggtag atcaatgaat ttacataatg
     1621 tgctttttcc cgcacccgac tctccaacca acgtaattaa ggtgggttta ttttgtacaa
     1681 agttttgttt gattgattta agtttattta gcattattta aaatctttat taataggttt
     1741 atccatttta ttaatttccg gattatatgt tctagtttcg ctatattcca gttttttctt
     1801 cataacctct tctaattttt caacaccgtg aaaatcttcg gcaatatttg caactttaca
     1861 agcaaaaacg attacatcag taacttcagc tggaacttct tcccatttct tttctaaaat
     1921 agccttacaa acttcgccaa cttcttcaga caattttaat gtcgcaatct taatagattt
     1981 ttcttcatct ttagcaatac ccttccttaa agaaaaatcc ctttcccatt tttgaatatc
     2041 agaaatattc atatttttat ttttaattaa taaaagattg ttcgattata tcactaaata
     2101 attacttttt aaaaa
//
LOCUS       DAOTFA010000070         2115 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate AMR_MDS_3115
            NODE_44848_length_2115_cov_5.752427, whole genome shotgun sequence.
ACCESSION   DAOTFA010000070 DAOTFA010000000
VERSION     DAOTFA010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868139
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (wastewater metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:36:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 536
            CDSs (total)                      :: 521
            Genes (coding)                    :: 520
            CDSs (with protein)               :: 520
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44848_length_2115_cov_5.752427"
                     /isolate="AMR_MDS_3115"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(86..1174)
                     /locus_tag="P5014_02465"
     CDS             complement(86..1174)
                     /locus_tag="P5014_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY57006.1"
                     /translation="MFKFLKQKKYFSAELKKAWMLIKKLFSLKAIKIILVILLFLLLA
                     PFILFFILRPKVQKNINYGVTFSNRFCEQMGLNWREVYVKSLDELYIKNIRLVAYWDE
                     IENERDSYDYSNIKWQLEEAEKRNVNVLLSIGRKVPRYPECFEPKWWDELPGENLKEQ
                     ELLEYIKQTVNELKGFKAIKIWQVENEPFWPFGDCQNPEIKKETVLKEIELTRSLDSR
                     PILVQDSGEGGVWRPTYEMGDYLGISMYRRIWYDFWGVFLGRFIYFQYPLAHWTYKIK
                     ANIVGVPVDKIIITELQGEPWGPRINSELTQEEKDETMSKNHFLSTLDYAQKSGFKDL
                     YLWGVEWWYFEKENGNPFYWDTAKALFR"
     gene            complement(1186..>2115)
                     /locus_tag="P5014_02470"
     CDS             complement(1186..>2115)
                     /locus_tag="P5014_02470"
                     /inference="COORDINATES: protein motif:HMM:NF012457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HRY57007.1"
                     /translation="KFDYREIAHYKKVLSAAKDRGLKTFVTLHHFTNPLWFYKKGGWI
                     NIKSPHYFSRYAKECAKEFGALADVYLTINEPQVYALMSYTVGKWPPNKSNLFLSLIV
                     QLNLMSAHNEAYKKIKSVGKYTVGLVKQVVWYETFEQKPYFWDSWYAKIVFWLNCDFF
                     LKFIKNNLDVVGLNYYFTNQIKGLRATHPKGMVSNLNWWINPDGLKNVLLHLKKYDLP
                     IYITENGLADSQDRLREMFIKNMLTATYEAMQKGSQVKGYFHWSLIDNFEWHEGYWPK
                     FGLVAIDRKTLERKPRKSFYYYAKICNNNSLDE"
BASE COUNT          624 a          382 c          337 g          772 t
ORIGIN      
        1 gtgcttcatc acccagaata agaagtgtgt gtgatcttta tattgaattt gaagatgtcg
       61 gtgacttgga aagttctttg tctgattatc taaacagtgc ttttgcggta tcccagtaaa
      121 aaggattccc gttttctttt tcaaaatacc accactccac cccccaaaga tataggtctt
      181 taaaccctga cttttgagcg taatcaagcg tggatagaaa atgatttttt gacatagttt
      241 catctttttc ttcttgtgta agttcagaat taattctcgg tccccaaggt tcaccttgaa
      301 gttcggtaat aattatttta tctacgggaa ctccgacaat gttagcctta attttataag
      361 tccagtgggc caatggatat tggaagtaaa taaatcttcc taagaatact ccccaaaagt
      421 cataccaaat cctcctatac atagatatac caaggtaatc acccatttca taggttggcc
      481 tccaaacccc gccctcgccg ctgtcttgaa ctaaaattgg ccttgagtcc agacttcttg
      541 ttaattcgat ttcttttaat acagtttctt ttttaatttc gggattttgg caatctccaa
      601 atggccaaaa tggttcgttt tctacttgcc aaattttaat ggctttaaaa cctttcagtt
      661 cgttgacggt ttgtttaata tattctaaca attcctgctc ttttagattt tctcccggaa
      721 gctcatccca ccacttgggt tcaaaacatt ctgggtatct tggtactttt cttccgatag
      781 aaagtaaaac atttacattt cttttttcag cttcttcaag ttgccattta atatttgaat
      841 agtcgtagga atctctttca ttttctatct catcccaata agcaaccaat ctaatatttt
      901 ttatataaag ctcatctaag gatttaacgt atacttctct ccaatttagt cccatttgtt
      961 cacaaaatct gttagaaaaa gttactccgt aattaatatt tttttgtact ttaggtctta
     1021 ggataaaaaa taaaataaat ggggcgagta gtaaaaacag aagtattact aagattattt
     1081 ttatagcttt tagcgagaat aattttttta taagcatcca agctttttta agttctgcac
     1141 tgaagtattt tttttgtttt aaaaatttaa acatatttat atcactcatt catccaatga
     1201 attattattg cagatttttg cataataata aaaggatttt ctcggttttc tttctaaagt
     1261 ttttctatct attgcgacaa gtccgaattt aggccagtag ccctcgtgcc actcaaagtt
     1321 gtctattaat gaccaatgaa aatatccctt aacctgtgaa cccttttgca tggcttcgta
     1381 tgtggcggtt agcatatttt ttataaacat ttctctaagt ctgtcttgag aatctgcaag
     1441 gccgttttcg gttatgtaga taggaagatc atatttttta agatgcaaaa gaacgttttt
     1501 caaaccatcg ggattaatcc accagtttaa gttactcacc attcccttag gatgagttgc
     1561 ccttaaacct ttaatttgat ttgtaaaata gtaattaagg ccgacgacat cgagattatt
     1621 tttaataaat tttaaaaaga aatcacagtt taaccagaaa actatttttg cataccagga
     1681 atcccagaag tatggttttt gttcaaatgt ttcataccaa acgacttgtt ttacaagtcc
     1741 tacggtatat tttccaaccg atttaatttt tttatacgct tcgttatggg ctgacattaa
     1801 atttagttga actattagtg ataaaaacag attagatttg tttggcggcc atttaccaac
     1861 tgtatagctc ataagtgcgt aaacttgagg ctcgttgatt gtaaggtaaa catcagcgag
     1921 tgctccaaat tctttagcac attcttttgc gtaacgggaa aagtagtgag gggatttgat
     1981 gttaatccaa ccaccttttt tataaaacca aagaggattt gtgaagtgat gtagtgttac
     2041 aaaagttttt aaacctcggt ctttggctgc tgataatacc tttttgtaat gagcaatttc
     2101 tcggtaatca aactt
//
LOCUS       DAOTFA010000071         2018 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate AMR_MDS_3115
            NODE_48539_length_2018_cov_6.702496, whole genome shotgun sequence.
ACCESSION   DAOTFA010000071 DAOTFA010000000
VERSION     DAOTFA010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868139
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (wastewater metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:36:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 536
            CDSs (total)                      :: 521
            Genes (coding)                    :: 520
            CDSs (with protein)               :: 520
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_48539_length_2018_cov_6.702496"
                     /isolate="AMR_MDS_3115"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            61..459
                     /locus_tag="P5014_02475"
     CDS             61..459
                     /locus_tag="P5014_02475"
                     /inference="COORDINATES: protein motif:HMM:NF023395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HRY57008.1"
                     /translation="MENCIFCKISKGEIPCMKIWENEKYLAFLDINPVSEGMTLVIPK
                     EHKDSRIFKNENSDVCEIMNASKEVANILENKLKIERVGLIFEGMEVDHLHAKLIPIK
                     GGENIRMILESHYPRPDMKGLEDLHKKIVD"
     gene            complement(473..1015)
                     /locus_tag="P5014_02480"
     CDS             complement(473..1015)
                     /locus_tag="P5014_02480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY57009.1"
                     /translation="MNYKKCNIYKFLVASFLSTILFSNVFAASLSLLKIGALDTGGKS
                     YPEWWYVGTGPVLSGMATPGSDVSVKIGENSYTTTPDASGNWSYSATLEKGDYPIEIS
                     QSGEKISFTLHMGQDMAATGATQPSDTTPDATAVPETGYNQYVAFSFGIGIILLATYF
                     YFSSDNKKNIVFESRVLKED"
     gene            1128..1802
                     /locus_tag="P5014_02485"
     CDS             1128..1802
                     /locus_tag="P5014_02485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY57010.1"
                     /translation="MDNLDIQPELPYPDESKSDKLNEENKSDCTEIKESVIEIQSALS
                     NLEFGDSNNKNQPLLNVNKESISSQLVSQIGDTNEKTVGVSVENDGKKVLVPDLDRED
                     AYLLHNILKEREGWESGSWIIFKKAEFRSRGRLTYNAVERLRKKYCIVDHKRAGKNYY
                     KWNDRYVFNESDLKETTNQQTPKNTESEDWWNRGPIISLADADFRYEMDKELENDKRD
                     KQQSTE"
     gene            complement(1839..>2018)
                     /locus_tag="P5014_02490"
     CDS             complement(1839..>2018)
                     /locus_tag="P5014_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015019638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY57011.1"
                     /translation="VKMFENHKTSKEGIFVSGDVHDHIYRQAISAAGYGCQAAIDVTR
                     FLSDHGIETHVSSYE"
BASE COUNT          754 a          305 c          364 g          595 t
ORIGIN      
        1 attagacaga tacgaagcag ataataaaaa tttatattta atttttggta gaatctgaat
       61 atggaaaatt gtattttttg taaaatatca aaaggtgaga ttccctgtat gaaaatctgg
      121 gagaacgaaa aatacttagc ttttttagat attaatccgg tttctgaagg tatgacctta
      181 gttattccaa aagagcacaa agattcaaga atttttaaaa atgagaattc agacgtctgc
      241 gaaattatga atgcttccaa agaggtggct aatatacttg agaataaact taagattgaa
      301 agggtggggt taatatttga agggatggaa gtagatcatc tccatgcaaa actaatacct
      361 attaaaggtg gagaaaatat aagaatgata ttggagtcac attacccaag accagatatg
      421 aaaggtttgg aagacctcca taaaaaaata gtagattaat tttttataag atttaatctt
      481 cttttaatac tctggattca aaaactatgt ttttcttgtt atctgaagag aagtaaaaat
      541 atgtagctag aagtattatc ccgataccaa atgaaaaggc tacgtattga ttataaccag
      601 tttcgggaac agcggtggcg tccggtgttg tgtcgctagg ttgagtagcc ccagttgcag
      661 ccatatcctg acccatatgc agagtaaaag atatcttttc cccactttga gaaatctcaa
      721 tcgggtaatc tcccttttct aaagttgcag agtaactcca gtttccggaa gcatcggggg
      781 ttgtagtata tgaattttca ccaattttta cactaacatc agaaccggga gttgccatac
      841 ctgataaaac cggtcccgta cctacgtacc accattcggg ataagactta cctccggtat
      901 cgagtgctcc tatttttaat aaagatagag atgctgcaaa aacattagaa aaaagtatag
      961 ttgataaaaa ggaagctaca aggaatttat aaatattaca ttttttataa ttcatacctt
     1021 aattaatgtt actacttttg tagtttatta atcaagatac cccggtccaa aatatttaaa
     1081 ttaatataca gtaataatca taattaatta gtgttaacat tagatatatg gacaatttag
     1141 atattcaacc agaattaccc tatcccgatg aaagtaaatc tgataaatta aatgaagaaa
     1201 ataaatccga ttgtacagaa attaaagaat cagtgatcga aatacagtct gcattatcta
     1261 atcttgaatt tggagacagt aataataaaa accaaccact ccttaatgtt aataaggaga
     1321 gtatatcttc acaattagtt agtcaaatag gagatacaaa tgaaaaaaca gtaggtgttt
     1381 cagtcgaaaa tgatggtaaa aaggtactag tacctgattt agacagagaa gatgcgtacc
     1441 ttcttcataa tatattaaag gaacgagagg ggtgggagag cggttcctgg attattttta
     1501 aaaaagcaga atttcggagt agaggtaggt taacctataa cgctgtagaa aggctcagga
     1561 aaaaatattg tatagtcgac cacaaaagag ccggaaaaaa ttattataaa tggaacgata
     1621 ggtatgtttt taatgaatcc gatttaaaag aaacaactaa tcagcaaact ccaaaaaata
     1681 ctgaatctga agattggtgg aataggggac caattatttc tctggcagat gctgatttca
     1741 gatatgaaat ggataaagaa ctagaaaatg ataagagaga caaacaacaa agtacagaat
     1801 aaaaaacaga ctttaccgct ttataatata ttcaaacatt actcatatga actaacgtgc
     1861 gtctcaatcc catgatcaga taaaaatctt gtaacgtcaa ttgcggcctg acatccataa
     1921 cctgcagcac taattgcttg tctatatata tgatcgtgaa cgtcgccgga tacaaaaatc
     1981 ccttctttcg atgttttatg attttcaaac atctttac
//
LOCUS       DAOTFA010000072         1996 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate AMR_MDS_3115
            NODE_49418_length_1996_cov_4.970118, whole genome shotgun sequence.
ACCESSION   DAOTFA010000072 DAOTFA010000000
VERSION     DAOTFA010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868139
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (wastewater metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1996)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:36:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 536
            CDSs (total)                      :: 521
            Genes (coding)                    :: 520
            CDSs (with protein)               :: 520
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1996
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49418_length_1996_cov_4.970118"
                     /isolate="AMR_MDS_3115"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..701
                     /locus_tag="P5014_02495"
     CDS             <1..701
                     /locus_tag="P5014_02495"
                     /inference="COORDINATES: protein motif:HMM:NF016119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HRY57012.1"
                     /translation="IYIKDESFDPIFNSVNPAFFFRTRVLPGLLMALGFIVLGTQVVL
                     PLVFFKTQDTVVKPMENTVLGLATGFKDFNFEEIEENNFYKENSSIPEYFYITIPKLK
                     IENALVKSEFDGPSPDDFIGHYKGSALPGEVGDSFIYGHSVLPWFFNPKNYKTIFSTL
                     GNLETGDRFTIEYNQRKINYKVEDKVVLSPKEVNPLETWKPAYLNQPTVSLMTCWPAG
                     TKTNRLIIRAVQEN"
     gene            complement(709..1203)
                     /locus_tag="P5014_02500"
     CDS             complement(709..1203)
                     /locus_tag="P5014_02500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY57013.1"
                     /translation="MDQNNNAYSIKTQSTKGLSTFVLTLSISLIVFSAIYYFMTTKAS
                     TSEYDAFNVSVVDETKSEESSSKPVTQKNTDEGSSVFGKLASSSQDSNSGQVLAGTDV
                     VETEVPAEVPQTTQSNLETGVTSITFGLISALALFVSGMVFIHKGPRKAALSTFEKNI
                     TGRI"
     gene            complement(1190..1381)
                     /locus_tag="P5014_02505"
     CDS             complement(1190..1381)
                     /locus_tag="P5014_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY57014.1"
                     /translation="MKLFFTTLGIFITVLLVWLVIGMKKIDRNTFLPSAVLDKAVPLN
                     AIIDIEFINNLKNPANGSK"
     gene            complement(1378..1932)
                     /locus_tag="P5014_02510"
     CDS             complement(1378..1932)
                     /locus_tag="P5014_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY57015.1"
                     /translation="MKKTDEFLNLIRRILSNESISIKIYIVWGLVTLLMFGFFGFLPA
                     SKIFLSNISLLDDLYQNNLNLEKKIDELKVAKERVELVGEDVNILDKFLPDEFQAQNY
                     LVDMSVIFGQSGYSLDSISFTNKDAHEVNMSLRISGKGNLKDLVSKLEESKKLNEIQS
                     IRYSVGSREDSLNMTIKSFIMENK"
BASE COUNT          658 a          364 c          323 g          651 t
ORIGIN      
        1 aaatttacat aaaggatgag tcttttgatc cgatatttaa ttcggtaaat ccggcattct
       61 ttttcagaac cagagtactt ccggggttat tgatggcgct ggggtttata gttttaggaa
      121 ctcaagtggt gcttccgctg gtatttttta aaacccaaga cactgttgta aaaccgatgg
      181 aaaacactgt cttaggtcta gctacagggt ttaaggattt taacttcgag gaaattgagg
      241 agaataattt ttacaaagaa aacagctcga ttccggaata tttttacatc actattccaa
      301 aattaaaaat tgagaacgcc cttgttaaaa gtgagttcga cggtccgagc ccagatgatt
      361 ttatcgggca ctacaaagga tccgcactac ccggagaggt tggagactct tttatttacg
      421 gccactcggt tttaccttgg ttctttaatc ccaaaaatta taaaacaatc ttctctactc
      481 tcggtaattt agaaaccggc gatagattta caatagaata caaccaaaga aaaattaact
      541 acaaggttga ggataaagta gttctctctc ctaaagaggt aaatcctttg gaaacctgga
      601 aacctgctta tctcaatcaa cccacagtta gtttaatgac ttgctggcca gcaggaacaa
      661 aaacaaacag gttgattata agggctgttc aggaaaatta aattcggatt aaattcttcc
      721 ggtaatattt ttttcaaaag tacttaaagc ggcttttctc ggacccttgt gtataaaaac
      781 cattcccgat acgaacaaag cgagagctga aattaaacca aatgtaatac ttgtcacacc
      841 tgtttcaaga ttactttgag tagtttgagg aacttcagcc ggtacttcag tttcaacaac
      901 atcggttcca gctaaaacct gtccggaatt agaatcctgt gaagaactcg ctaactttcc
      961 gaaaacagaa ctaccctcat ccgtattttt ctgagtaacg ggttttgaag aggattcctc
     1021 tgacttggtt tcatcaacaa cactaacatt aaaagcgtcg tattcggacg tagatgcctt
     1081 tgttgtcata aaataataaa ttgcactgaa gacaataagc gaaattgaaa gcgtgaggac
     1141 aaaggtgctt aaaccttttg ttgactgtgt ttttatgctg tatgcgttat tattttgatc
     1201 cattggcagg atttttcaaa ttattaataa attctatatc aatgatagca ttcaaaggta
     1261 cagctttgtc aagtactgct gaaggaagaa aggtgtttct gtcaattttt ttcataccaa
     1321 taactaacca gacaagtaat actgtaataa atattccaag tgtagtaaaa aataatttca
     1381 tttattctcc ataataaaag acttgatagt catattcaaa ctatcttctc tgcttcccac
     1441 gctatatcta atactttgga tctcattcaa tttttttgac tcttctaatt tagataccaa
     1501 atcctttaaa tttccttttc cggatatacg aaggctcata ttgacttcgt gagcatcttt
     1561 attagtaaaa cttattgaat ccagagaata accggactgg ccaaatatta cagacatatc
     1621 cactaaataa ttttgggctt gaaactcatc gggaagaaac ttatctaaaa tattgacatc
     1681 ttctccgact aattcaactc tttcttttgc cacctttaac tcgtctattt tcttttccaa
     1741 gttaaggtta ttttgatata aatcatctag aagagaaatg ttggataaga aaatttttga
     1801 tgccggtaaa aatccgaaaa aaccaaacat taataaggtt actaaacccc atacaatata
     1861 aatcttaata gagatagatt cgtttgaaag aattctcctt attaaattta aaaattcatc
     1921 tgtttttttc attatttaaa attacctctt aacataacct tatatatatt ctccgactta
     1981 tctaaacttg ctgaat
//
LOCUS       DAOTFA010000073         1986 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate AMR_MDS_3115
            NODE_49853_length_1986_cov_6.480580, whole genome shotgun sequence.
ACCESSION   DAOTFA010000073 DAOTFA010000000
VERSION     DAOTFA010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868139
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (wastewater metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1986)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:36:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 536
            CDSs (total)                      :: 521
            Genes (coding)                    :: 520
            CDSs (with protein)               :: 520
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_49853_length_1986_cov_6.480580"
                     /isolate="AMR_MDS_3115"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1504
                     /gene="dnaX"
                     /locus_tag="P5014_02515"
     CDS             <1..1504
                     /gene="dnaX"
                     /locus_tag="P5014_02515"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR02397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HRY57016.1"
                     /translation="SQMSVFHLKYRPHKVTDLDLPEVSDKINSIITSETDFQSLLFAG
                     PKGSGKTSAARIIAKAVNCLKIKKGEACGECENCLEIEKGNSLDIIEIDAASNRGIDD
                     VRGLKENAYLSPIRLKKKIFIIDEVHMLTKEAFNALLKIIEEPPKNTLFILCTTDAQK
                     IPETVLSRLLKVNFRKGKEEDLERSLAKVIKGEKIEISDEAKKEIIINSDGSFRNLQK
                     TFNEIILQFGSKIGKEEVEKYFMASRGGYSGEELEKDLQAGEIKIIVEKLEKMAEKGV
                     DFGELRLKWLEYFHQKMLVDLNNKEINKWIEILIRSGEMEKMAIIEQLPLELAVIEMV
                     EKKESLKPKEEIEQKTEESLKPKEEVKEKKEESLKPKEGIKEVKVELVEVEEKWGNVL
                     GAVKPYNHSVEAFLRAARPKKIKDGKLLIEVFYKFHKEKLEEEKNRKIVEMGLLKVFG
                     GAVRFDCVLAEADSKRTMPMVNVNKEVKDSRDRPVASESEIYDVAKNIFG"
     gene            1504..1854
                     /locus_tag="P5014_02520"
     CDS             1504..1854
                     /locus_tag="P5014_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HRY57017.1"
                     /translation="MTAHSSETQQKLFCLQEGKSPFSGLPLDRINQRFEIHHVKERVN
                     GGGNSLINLQLLPIYEHLAIHFIKFRDKKLSEEERLREFDIVMGRLNQLTEEEKNIFY
                     QLVEEKVGIRVRFY"
BASE COUNT          791 a          215 c          444 g          536 t
ORIGIN      
        1 tagccagatg tcagtttttc atttgaaata ccgtccacac aaagtcacgg atttagattt
       61 acctgaagta tcggataaaa ttaatagtat tattacgagt gaaaccgatt tccagtcttt
      121 gctttttgcc gggccaaagg ggagtggaaa aacttcagcc gccagaatta ttgccaaggc
      181 cgtaaactgc cttaaaataa aaaaaggtga agcctgtggg gagtgtgaaa actgtctgga
      241 aatcgaaaaa gggaacagtc tcgatattat tgaaattgat gccgcttcta atcgaggaat
      301 agatgatgtt aggggtttaa aggaaaatgc ttatttgtcg ccaattagac ttaaaaagaa
      361 aatttttatt attgatgagg ttcacatgct gactaaggaa gcttttaatg ctcttttaaa
      421 gataattgag gagccaccca aaaatactct gttcatttta tgtacaactg atgcccagaa
      481 aattccagag acggttttgt cgcgactcct taaagttaat tttagaaaag gaaaagaaga
      541 agatttggag agatctttgg ctaaagtaat caagggtgaa aaaattgaaa taagtgatga
      601 ggctaaaaaa gaaataataa ttaattctga cggcagtttt agaaatttac agaaaacttt
      661 taacgagata attcttcagt ttggcagtaa gataggcaaa gaagaagtgg aaaaatattt
      721 tatggcaagt cgggggggat atagtggaga agagctagaa aaagatttgc aggcgggaga
      781 gataaaaata atcgttgaaa aactggagaa aatggccgaa aaaggagttg attttggaga
      841 actgagatta aaatggcttg agtattttca tcaaaagatg cttgtcgatt tgaataacaa
      901 agaaatcaat aaatggatag agatattgat aagaagcggg gaaatggaaa aaatggcgat
      961 aattgagcag ttaccattgg aactcgcggt aattgaaatg gtggaaaaaa aagaaagtct
     1021 aaagcctaaa gaagaaatag aacaaaaaac agaagaaagc ctaaagccta aagaagaagt
     1081 aaaagaaaaa aaagaagaaa gcctaaagcc taaagaggga ataaaagagg tgaaggtaga
     1141 actggttgag gtagaggaaa aatggggaaa tgtactagga gcagtcaagc cttacaatca
     1201 ttcagtagag gcctttttaa gagctgctag accaaaaaaa ataaaagacg gtaagctact
     1261 gattgaagtt ttttataaat ttcacaaaga gaaattagaa gaagagaaaa atagaaagat
     1321 tgtagaaatg ggtcttttaa aagtttttgg aggagcagtt agatttgatt gtgtgttggc
     1381 tgaggctgat tctaaaagaa cgatgccaat ggtgaatgta aataaagaag taaaggacag
     1441 tcgggaccgt cccgtagcat cagaatcgga aatttatgat gtggctaaaa atatatttgg
     1501 ataatgacag cacacagttc agaaacacaa caaaaattat tttgtttaca ggaaggtaaa
     1561 tcaccatttt ctggtttacc tttagataga ataaatcaaa gatttgaaat tcatcatgtt
     1621 aaagagcgtg ttaacggagg aggaaattct ttaataaatt tgcaattatt gccgatttat
     1681 gaacatttag caattcattt tataaaattt agagataaaa aattatccga agaagagaga
     1741 ttgagagaat ttgatattgt aatgggtaga ttaaatcaat taactgaaga agaaaaaaat
     1801 attttttatc aattagtaga ggagaaagtt gggataagag ttagatttta ttagttatta
     1861 agaagtgata gaattaggag ttatttaatt tttaataaaa aaatatgttt gataaagcaa
     1921 aattgatggc gaaggctttt aaattaaaaa aggctttaga gttggaacta actgaacttg
     1981 aagaaa
//
LOCUS       DAOTFA010000074         1978 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate AMR_MDS_3115
            NODE_50223_length_1978_cov_8.537181, whole genome shotgun sequence.
ACCESSION   DAOTFA010000074 DAOTFA010000000
VERSION     DAOTFA010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868139
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (wastewater metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1978)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1978)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:36:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 536
            CDSs (total)                      :: 521
            Genes (coding)                    :: 520
            CDSs (with protein)               :: 520
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1978
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50223_length_1978_cov_8.537181"
                     /isolate="AMR_MDS_3115"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1686
                     /locus_tag="P5014_02525"
     CDS             <1..1686
                     /locus_tag="P5014_02525"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRY57018.1"
                     /translation="GLYISRDTGVTWEKVLGFGNYSVDKMIKFENKYYAGRYDEEVQK
                     YSVFVSTNEGLSWENAGLNSSTINKVFSLATIYAEPPILFSSISNMGVFKTEIPRSGL
                     VFNSFLNTPWNYKNENELVDKINSFFDHSYPLLGYTYHPEPENETSTTLNFLGLRSAE
                     PEIYYSSHSGYDFGLDFGTPVIASSSGYASYYYCKDCGNSIKINHLNGFLTTYMHLQE
                     DNILTKEGPVWVNAGDIIGKVGLTGRTTGPHLHFEVTKDSDGDGNFNDDFPTGRTDPF
                     GWQCTQKNDPWENYSWEDALGSHLGTQSQYLWNYMNGNEIRTMSPDSNPNEIVLENKH
                     AIFENPIYLFTTKMISYVQPSKNTFGQILKYINNTSFILKSFDQTGENITNFINPVFI
                     EIFVDPENLTGVNLNSISLYFYNEVSNVWEKVPSSFNDQLNKISATVNHLSWFAVFGE
                     KENSAPVSTGIIISGSQNDIWFTEPPLVEFFIENQENPQNISTFYSLDNGDSWQLYEA
                     PFYINTEGINILIYKSQGSDGLMEEEHEQVIYVNLSNKITGKIRVSGAIFQTE"
     gene            complement(1709..>1978)
                     /locus_tag="P5014_02530"
     CDS             complement(1709..>1978)
                     /locus_tag="P5014_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY57019.1"
                     /translation="GDEIRIGRLLNPNYEDNTSVYGKVCGQTKGGKQIAQKLWDAWKR
                     GRNTVFHYFPHNFKKLEYNEALDIINDVINAMSQVISVCLDKKTR"
BASE COUNT          680 a          349 c          346 g          603 t
ORIGIN      
        1 ggtctttata tttccagaga taccggagta acttgggaaa aagtacttgg gtttggcaac
       61 tattccgtag ataaaatgat taaatttgaa aataaatact acgccggaag atatgatgag
      121 gaagtacaaa aatattcagt atttgtatca acaaacgaag gtctttcatg ggaaaacgca
      181 gggctaaact cttctactat aaacaaagtg tttagcttgg caactatata cgcagaaccc
      241 ccaatactat tttcatctat atctaatatg ggagttttta aaacagaaat tcccagaagt
      301 ggtttagtgt ttaactcctt tttaaatact ccgtggaatt ataaaaatga aaatgaacta
      361 gttgataaga ttaattcgtt ctttgatcac tcctaccctt tacttggata cacttatcat
      421 ccagaaccag aaaatgaaac cagtacaact ttgaattttc ttggcttaag aagtgccgaa
      481 cctgaaattt attatagttc acacagtggt tatgattttg gtttagattt tggaacccct
      541 gttattgctt cttcttcagg ttacgcttcg tattattact gcaaagattg tggaaattca
      601 ataaaaataa accacctaaa cggttttcta acaacataca tgcatttaca agaagataat
      661 attttaacta aagaaggacc tgtttgggta aacgcgggtg acataattgg aaaggtcggg
      721 ttaaccggaa gaactacggg accgcacctg cactttgagg ttactaaaga tagtgatggg
      781 gatggcaatt ttaacgatga ttttcccaca ggtaggacag acccattcgg ttggcagtgt
      841 acgcaaaaga acgacccgtg ggaaaattat tcttgggaag atgctttagg atcacacctg
      901 ggaacgcaga gtcagtatct ttggaattat atgaacggaa acgaaatcag aacgatgtct
      961 cccgatagta atccgaacga aatagtttta gaaaataaac acgctatttt tgaaaacccg
     1021 atatatcttt tcactacaaa aatgataagt tacgtacaac caagtaaaaa cactttcggt
     1081 caaattctaa agtacatcaa caacacctca tttattttaa aatcttttga tcaaacaggg
     1141 gaaaacataa ctaatttcat aaatccggtt tttatagaga tatttgtaga tcctgaaaat
     1201 cttactggtg taaaccttaa ctccataagt ctttactttt ataacgaagt atcaaatgtt
     1261 tgggaaaaag ttccaagttc gttcaatgat cagttgaata aaattagtgc tacagtgaac
     1321 caccttagct ggtttgctgt atttggagaa aaagaaaatt cagcgcctgt ttctactgga
     1381 attataatat ccggaagtca aaacgatatc tggtttacag aacctccgct tgtagaattt
     1441 tttatagaga accaggaaaa tccacaaaat attagcacat tctattcttt ggataacgga
     1501 gatagctggc aactatatga agcgcctttt tacattaaca ctgaaggtat caatatctta
     1561 atatataaat cgcagggttc cgatggattg atggaagaag aacacgaaca agtaatttat
     1621 gtaaatcttt ctaacaaaat aaccggaaaa attagagtta gcggggctat tttccaaacc
     1681 gagtaaattg aaaattaaag ttcaataatc agcgcgtttt cttatccaag caaactgaaa
     1741 taacctggga cattgcgttt ataacatcat tgataatatc taaagcttcg ttgtactcca
     1801 attttttaaa attatgagga aaataatgaa aaacagtgtt tctacctctc ttccaagcat
     1861 cccaaagctt ttgtgcaatc tgtttaccac cctttgtctg accgcaaacc tttccgtaaa
     1921 cactcgtatt atcttcataa ttcgggttta aaagcctccc tattctgatt tcatcgcc
//
LOCUS       DAOTFA010000075         1973 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate AMR_MDS_3115
            NODE_50433_length_1973_cov_7.492701, whole genome shotgun sequence.
ACCESSION   DAOTFA010000075 DAOTFA010000000
VERSION     DAOTFA010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868139
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (wastewater metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1973)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1973)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:36:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 536
            CDSs (total)                      :: 521
            Genes (coding)                    :: 520
            CDSs (with protein)               :: 520
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1973
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50433_length_1973_cov_7.492701"
                     /isolate="AMR_MDS_3115"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..503
                     /gene="uppS"
                     /locus_tag="P5014_02535"
     CDS             <1..503
                     /gene="uppS"
                     /locus_tag="P5014_02535"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HRY57020.1"
                     /translation="NWKRSKEEVDKLFDIIEGMAKKYIDRCGKEKVRFVHIGRKDRIP
                     ATLKDLIIKAEKDTKQFIDFTIVLAIDYGGHDDLLRAIDKTIKSKRAVTSENIEKNLD
                     TAELPQIDYIIRTGGEKRLSGFMSWQCAYAELFFTDTYFPAFTPKKFEDAIKEFSRRV
                     RRFGGN"
     gene            667..1308
                     /locus_tag="P5014_02540"
     CDS             667..1308
                     /locus_tag="P5014_02540"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014447.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRY57021.1"
                     /translation="MIVITGDKKNELDKETFRNICGKYEKVILDLGTGDGRFVFKNAL
                     KNKSTLYIGLDPAEKQIQIYSKKSNRRRLKNALYVIGSLENLPDELYSTVDKIFINLP
                     WGTLLEKIVKSNETYTNELFKILKKNGEIEIIFGYVPELEPSETERLDLPVIENESDV
                     LKAFSTFKKIFEVIEMKRLLKDELEKIETTWAKKLKFGKDRFIYKIVLRKIAV"
     gene            complement(1310..1570)
                     /locus_tag="P5014_02545"
     CDS             complement(1310..1570)
                     /locus_tag="P5014_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY57022.1"
                     /translation="MEHGDIIQEETKTDVSSNTQSVDDKKEKDSEFPGQEIEHPTIKE
                     ASKEDLEEIKKVKPVIIEWKKPFFIMFFLGVAAVALTYFLRL"
     gene            1648..>1973
                     /locus_tag="P5014_02550"
     CDS             1648..>1973
                     /locus_tag="P5014_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY57023.1"
                     /translation="MAYHVYKLAVDILEGGSNYLLRHLLDLNLIIGFEKEPVLEHGTG
                     VATFFVKVPNKRKFEAYFNRKWHENADPVSFAVGKPTYDSYKSDGKKDVGTKVREFIN
                     KSKAKKL"
BASE COUNT          729 a          286 c          375 g          583 t
ORIGIN      
        1 aaaactggaa gagatcaaaa gaagaagtag ataaattatt tgacataatc gaaggtatgg
       61 ccaaaaaata tattgatagg tgtggaaaag aaaaagtaag atttgttcac attggtagaa
      121 aagacagaat ccccgcaaca ctaaaagatt tgataattaa agcggaaaaa gatactaaac
      181 aatttattga tttcactatc gtgcttgcaa tagattacgg tggacacgac gacttactaa
      241 gagcaattga caagacaatt aagagcaaga gggcagtaac aagtgaaaac atcgaaaaaa
      301 acctcgatac cgcagaactt cctcaaattg actacattat aagaactggt ggggaaaaaa
      361 gactaagtgg atttatgtct tggcaatgcg cctacgcaga acttttcttt accgatacat
      421 atttcccagc ttttactcca aaaaaattcg aagatgcaat aaaagaattt tcgagacggg
      481 taagaagatt cgggggaaat taacagatta aactacacct tagcttattc ggtgcatctt
      541 tttcttggtc tttttgattt ttatgttcct ttgtttaaga tttttctttt tctttcttgg
      601 catagtgatg atattctgcc acattaatca aataattcaa ggtgtttttt atgatattat
      661 tgcgcaatga tagtaataac gggggataaa aagaatgagt tagataaaga aacatttaga
      721 aatatttgtg gtaagtatga gaaagtgatt ttagatctcg gtacgggaga tggaagattt
      781 gtttttaaaa atgccttaaa aaataaaagt accctgtata taggtttaga tccggctgaa
      841 aagcaaattc aaatttattc aaaaaaatct aacagaagaa gattgaaaaa tgccctttat
      901 gttatagggt cgttagaaaa cctacccgac gaactgtatt caactgttga taagattttc
      961 ataaatttac cttgggggac tcttctagag aaaatagtta aatcaaatga gacttacact
     1021 aatgaactat ttaaaatttt aaagaagaat ggtgagatag aaataatatt cggatatgta
     1081 ccggaattag aaccatctga gactgaaaga ctggacttac cagtaattga aaacgaatcc
     1141 gatgtattaa aggccttctc tactttcaaa aaaatttttg aggtaatcga gatgaaaagg
     1201 ctattaaagg atgaattgga aaagatagag accacatggg ctaaaaaatt aaagttcgga
     1261 aaagatagat ttatatacaa aatagtattg agaaagattg ctgtataggt taaagcctta
     1321 gaaaataagt gagggctaca gctgcaaccc ccaagaaaaa cattataaaa aagggttttt
     1381 tccactcgat tattacaggt ttaacttttt ttatctcttc caaatcctct tttgaggctt
     1441 cttttatagt cgggtgctca atctcctgac caggaaattc agaatccttt tcttttttat
     1501 catcaacact ctgagtatta ctacttacat cggttttagt ttcttcctga attatgtccc
     1561 cgtgctccat aaaacaatgc taccattttg aaaaaatcag gtaaatggga tattatgcat
     1621 aaaactctct agaaaggagg gaacgaaatg gcttatcacg tttataaatt ggcggtagat
     1681 attctggaag gaggttcgaa ctatcttctt cgacacctgt tggatttgaa tttaatcatc
     1741 ggtttcgaaa aagaacctgt tttagaacac ggtacagggg tcgctacttt tttcgtaaag
     1801 gtgcctaaca aaagaaagtt cgaggcatat tttaatagaa aatggcatga aaacgcagac
     1861 cctgtaagtt ttgcagttgg aaaaccaact tatgatagtt acaaaagtga tggaaaaaaa
     1921 gacgtaggaa caaaagtaag agagtttatt aataaaagca aggcaaaaaa act
//
LOCUS       DAOTFA010000076         1954 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate AMR_MDS_3115
            NODE_51247_length_1954_cov_5.624539, whole genome shotgun sequence.
ACCESSION   DAOTFA010000076 DAOTFA010000000
VERSION     DAOTFA010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868139
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (wastewater metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1954)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1954)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:36:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 536
            CDSs (total)                      :: 521
            Genes (coding)                    :: 520
            CDSs (with protein)               :: 520
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1954
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51247_length_1954_cov_5.624539"
                     /isolate="AMR_MDS_3115"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..579)
                     /locus_tag="P5014_02555"
     CDS             complement(<1..579)
                     /locus_tag="P5014_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY57024.1"
                     /translation="MKKQNFNLIKIFPAIVLVILFSRSTKVLADPLQNKSDTMSRLGN
                     SSSDAVLSDHEIQFTTPTGVASTQSIEITFPSDFDGLNDPQGALDYTDVDLLIDNTPD
                     GTCDGIDQTLVASGAGVSEWNAVFSDTENRVLTLTSGGASAVIGIGDEVCIKIGENAT
                     GGTANTQYINPTSSAAYTISIVAASDSGQISVS"
     gene            complement(756..>1954)
                     /locus_tag="P5014_02560"
     CDS             complement(756..>1954)
                     /locus_tag="P5014_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY57025.1"
                     /translation="KYNKFLIVLGVVLFSLVSFSLKNKAYALTQPTKVEIDGEPGKVF
                     SGTLELYNEQEQEITFYLSYQNFEARGEGGSPAFLDYDGTGLASWIKTSQSITLKPKE
                     RIEFPYEIQIPQDSEPGTYLAAIFFGNTPPQAMEGGQVTIGTKLGTLLFLNLPGAKVE
                     GAGVTEFSTEGGKFFSYLPLNLYYRFSNNSALKVMPFGSIDIKCLFGRTMGTLDANIS
                     KGNVLPSSVRKFVITWDKLRQDNVNEKEGKTEETTVKPGDKLSFFDAVKIQMKNFAFG
                     KYTATLNVKYGENLSATKQTSFWVVPWQLLSLILLALIIIILLFKGMMKRHNKKLIEM
                     VKKEALTSQQVTIAPAESIAKEEKKVHEKVKKEVKDNVEEIKETIKEETDGEAEETLT
                     ALNPKE"
BASE COUNT          514 a          382 c          355 g          703 t
ORIGIN      
        1 acttacggat atttgtcctg aatcagatgc agcaacaata gagatggtgt aagccgcact
       61 tgaggttggg tttatatatt gggtattggc tgtgcctcct gttgcatttt cacctatctt
      121 aatgcacacc tcatctccta ttcctataac tgctgaggct cctcccgagg tgagcgtaag
      181 tacgcggttt tctgtgtcgc tgaagacagc attccactca ctgactcctg caccggaggc
      241 taccagtgtt tgatctattc cgtcacaggt tccgtccggt gtgttgtcta ttagtaagtc
      301 gacatctgta tagtcaagcg ccccttgtgg atcgttaaga ccatcaaaat cagaagggaa
      361 agttatctct atagattgtg ttgaagctac tcctgttgga gtagtaaatt gtatctcgtg
      421 atctgaaagt acagcatcgc tgcttgaatt acctagtctg ctcatagtgt cagacttatt
      481 ctgtaaggga tctgctagaa ccttagtcga gcgggaaaat aaaattacca aaacgatagc
      541 aggaaatatt tttattaaat taaagttttg ttttttcatt acagctaata attctacaga
      601 gcagcgatat cctgtcaatt actttaacta ttcttaaatt cccccccccc gggggatttt
      661 atgaattaga aagacagtgt caaacatcaa aattaaatat caaaaaggcc cgccggaggc
      721 gggccttttg cttgtcctta cgttttttaa ttatactatt ctttgggatt taaagctgta
      781 agtgtttctt ctgcttctcc atcagtctct tcctttattg tctcttttat ctcttcaaca
      841 ttatccttga cttccttctt aactttttca tgtaccttct tttcttcttt tgcaatgctt
      901 tcagcgggag cgattgtaac ttgttgtgat gtcaaagctt ctttttttac catctcaata
      961 agttttttat tatgtctttt catcatacct ttaaataaaa gtattatgat tattaaagcg
     1021 agtagtatta aagagagtag ttgccacgga actacccaga atgatgtttg ttttgtagca
     1081 cttagatttt ctccgtattt aacatttaat gttgcggtat atttgccgaa ggcaaaattt
     1141 ttcatttgaa tttttacagc atcgaagaaa cttaatttat ctcctggttt tactgtagtt
     1201 tcttctgttt ttccttcctt ttcatttacg ttatcttgtc ttagtttatc ccaagtaata
     1261 acaaattttc tcacgctgct tggcaagacg tttccttttg agatgtttgc gtctaatgtg
     1321 cccattgttc ttccgaaaag acattttata tctatactac cgaagggcat tacttttaag
     1381 gcgctgttat ttgagaatct gtagtaaaga ttaagaggta ggtacgagaa gaattttcca
     1441 ccttctgttg agaattctgt aactcctgct ccttcaactt ttgcaccagg aagatttaag
     1501 aataacaacg ttccaagttt tgtacctatt gtaacttggc caccttccat cgcttgagga
     1561 ggtgtatttc caaaaaatat agctgctaag tatgttcccg gctctgaatc ctgaggaatt
     1621 tgaatttcat aagggaattc aattctttcc ttaggtttta aagttatact ttgtgatgtt
     1681 tttatccaag atgctaaacc agttccatcg taatccaaaa aggccggaga gccaccttcc
     1741 cctcttgctt cgaagttctg gtaggaaaga taaaaagtta tttcctgttc ttgttcgtta
     1801 taaagctcta gcgtacctga aaaaaccttt cccggttcgc catctatttc gactttggtt
     1861 ggttgagtta gcgcgtaagc cttgtttttt aatgaaaaag aaaccagtga aaataatacg
     1921 acaccgagaa ctattagaaa tttattatat tttt
//
LOCUS       DAOTFA010000077         1948 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate AMR_MDS_3115
            NODE_51503_length_1948_cov_6.553090, whole genome shotgun sequence.
ACCESSION   DAOTFA010000077 DAOTFA010000000
VERSION     DAOTFA010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868139
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (wastewater metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1948)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1948)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:36:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 536
            CDSs (total)                      :: 521
            Genes (coding)                    :: 520
            CDSs (with protein)               :: 520
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1948
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51503_length_1948_cov_6.553090"
                     /isolate="AMR_MDS_3115"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(214..1590)
                     /locus_tag="P5014_02565"
     CDS             complement(214..1590)
                     /locus_tag="P5014_02565"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019227097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HRY57026.1"
                     /translation="MEKIDNLQQKIVSLCKRRGFVYPGSEIYGGLANTYDYGPLGVLM
                     MRNIQNLWWEHFITKRPEIYGLDTAVFMSPEVWIASGHTANFTEVLVDCKNCKQRTGA
                     EKLIEAYFNEKGEEVVAGGKSAEELEKIIIDNKIPCPECGEFNWTKPRKFNNLFETHI
                     GIVPENQSLNYLRGELAQGMFVNFKNVLDSQRPKLPFGLGQVGKVFRNEITKGNFIFR
                     TLEFTLMEFEYFFNPKTRNWEDIFEYWKKEMNNWIISLGLSQDKLRWRVHTDEERAHY
                     SKKTEDLDYEFAVGFKEMFGLAYRTDFDLKNHMEKSGSDLQYMDPETGEKFIPHVIEP
                     TFGSSRIFLALLTQGYTEEEGRLVLKLNKRVAPYRVAVFPLVKNKEDLVNKAKEIYNS
                     LLEKGFSVDWDDRGNIGKRYFAQDEIGTPYCITVDYQTLEDGTVTIRDRDTMTQERVS
                     ISDISSKI"
     gene            complement(1608..>1948)
                     /locus_tag="P5014_02570"
     CDS             complement(1608..>1948)
                     /locus_tag="P5014_02570"
                     /inference="COORDINATES: protein motif:HMM:NF014607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA repair protein RecO C-terminal
                     domain-containing protein"
                     /protein_id="HRY57027.1"
                     /translation="SGDLYTSYFEINFMKILGYQMDFDKCRICGKKLDDSWKKHYFNV
                     ENGSIECGDCSKIGVNISKNAALSLNRISNGKLEKNDHKIFEEIDKILKMYVGRKLEN
                     KFKSLEIDLE"
BASE COUNT          631 a          394 c          233 g          690 t
ORIGIN      
        1 atatccaata tccaccattt agtcgttgtt gtcaatacaa ataaaacccg aagtattgat
       61 tttcataatc tacctatagg ttgtaaattc agaaataatt atttttataa gataaaggta
      121 tgaaagattt aaaaatatcg atgtgtactt aacataaaca atacaaagga tttaattagc
      181 acctactcgg cgatataaaa agataaaaga aaactatatt tttgaagaaa tatctgaaat
      241 actgactctc tcttgagtca tagtatctct gtctcttata gtaaccgtac catcttccaa
      301 agtctgataa tcaacggtaa tacaataagg agtacctatt tcatcctgag caaaatatct
      361 cttaccaata tttcctctat catcccaatc tacggaaaaa cctttttcta acaaactgtt
      421 ataaatctct ttggctttat taaccaaatc ctctttattt ttaacaagag ggaaaaccgc
      481 aactctgtaa ggagcaaccc ttttatttaa ctttaaaact aatcttccct cttcttcagt
      541 gtaaccctgg gttaacaagg ctaaaaatat tctcgaagaa ccaaaagtag gttcgataac
      601 atgaggtata aatttttcac cagtctcggg atccatgtac tgaagatcag aacctgattt
      661 ttccatatgg tttttaagat caaaatcggt tctgtaagct aaaccaaaca tttctttgaa
      721 acctaccgca aattcataat ccaaatcctc tgttttttta gaataatgag ctctttcttc
      781 gtcagtatga actctccacc tcaatttatc ttgtgataaa cctaaggaaa taatccaatt
      841 attcatttcc tttttccaat attcaaaaat atcctcccaa tttcttgttt tagggttaaa
      901 aaaatattcg aattccatta acgtaaactc taaagttcgg aagatgaaat ttcctttggt
      961 gatttcattt ctaaaaactt ttccaacttg ccccagcccg aacggtagtt ttggtctctg
     1021 tgagtctaaa acatttttaa aattaacaaa cattccctgg gctaattcac cacgtaagta
     1081 atttaaggat tgattctctg gtactattcc gatatgtgtt tcaaataaat tattaaattt
     1141 tctgggtttg gtccaattaa actcaccaca ctcaggacaa ggaattttgt tatcgattat
     1201 aattttttct aattcttctg ctgacttccc tccagcaacc acttcctcac ctttttcatt
     1261 aaaataagct tcgatcaatt tttcagctcc ggttctttgt ttacaattct tacaatcaac
     1321 taaaacttcc gtaaagtttg ctgtatgtcc tgaagcaatc caaacttcag gagacataaa
     1381 aactgcagtg tccaaaccat aaatttccgg tctttttgtt ataaaatgct cccaccaaag
     1441 attctggatg tttctcatca tcaatactcc caatggaccg taatcataag tgttagctaa
     1501 gccaccgtaa atttccgatc cgggatatac aaaacccctc cttttgcata gtgaaacaat
     1561 tttctgttga agattatcta ttttttccat gcacttagta tatcaaatca ttccaagtct
     1621 atctcaagac ttttaaactt attttctaac tttcttccaa catacatttt taaaatttta
     1681 tctatctctt caaatatttt atggtcattc ttctctagtt taccattcga aattctattt
     1741 aatgatagag cggcattttt agagatattt acacctatct tggagcaatc accacattca
     1801 atacttccat tttctacgtt aaaataatgc tttttccatg aatcatctaa ttttttaccg
     1861 caaattctgc acttatcaaa atccatctgg tatccaagta ttttcataaa atttatttcg
     1921 aaataacttg tgtataagtc ccctgaat
//
LOCUS       DAOTFA010000078         1901 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate AMR_MDS_3115
            NODE_53665_length_1901_cov_7.930661, whole genome shotgun sequence.
ACCESSION   DAOTFA010000078 DAOTFA010000000
VERSION     DAOTFA010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868139
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (wastewater metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1901)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1901)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:36:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 536
            CDSs (total)                      :: 521
            Genes (coding)                    :: 520
            CDSs (with protein)               :: 520
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1901
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_53665_length_1901_cov_7.930661"
                     /isolate="AMR_MDS_3115"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..728)
                     /locus_tag="P5014_02575"
     CDS             complement(<1..728)
                     /locus_tag="P5014_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY57028.1"
                     /translation="MKKKSCPKSNFFRIFLILFFLSFLFTIVIKDYFENFKLPKRPQS
                     IYAASNTFSTSGSWLTPTGIYRVKVECWGGGGAGGGITTNRTRGRGGGGAGGQYAARS
                     EISCIPGSYYSIVVGAGGTGSFISGQAGEDSTFDGNVVIAKGGAGGQSYESGYQPGIG
                     TTSGGAGDIVYAGGSGSVGGTGTTGGAGGGGAGSTGAGGNASGNTAGTGTTVDGGDGG
                     AGLATSANGNPGSIYGGAGGAGAST"
     gene            complement(788..1492)
                     /locus_tag="P5014_02580"
     CDS             complement(788..1492)
                     /locus_tag="P5014_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY57029.1"
                     /translation="MNKFLKTIIFSLALTTLANFSLAFVLPANEIKIGDPVYIEETDT
                     LKNYAVFLPFEEPTKGQVCASISGEELSENNNLKDYGTCFINDSGIFTIVEIEEPFSS
                     SYEELKNNEEILQEDTVTLSNDFESIVKQSTEEIEGVLSESSDSEGIISDLGSFIQEA
                     QDEISRILASLTDTQESSETTESQVLGLRTFNIMNLLQENHLVIILIILIAISTTILI
                     SLIKKIKDTKEKNKDK"
     gene            complement(1501..>1901)
                     /locus_tag="P5014_02585"
     CDS             complement(1501..>1901)
                     /locus_tag="P5014_02585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY57030.1"
                     /translation="SKAVVGGGSSTSITADAVCNPFGYSVCNTTQTTMFGFAVPYNAV
                     IKNIYGNLQTAPAAGTTCAFTVRSSASCTGAYTATALTCTVTGNGSNRNCSNNSNEVS
                     ISAGDCLQIHYDETGTCSGIINWGFEIGPQ"
BASE COUNT          523 a          423 c          337 g          618 t
ORIGIN      
        1 gttgacgctc cggcgcctcc ggcgcctccg taaatactac ccgggttacc gtttgcggat
       61 gtggctagac ctgcacctcc gtctccccca tctactgttg ttcctgttcc tgcagtattt
      121 ccactggcgt ttcctcctgc acctgtagaa ccggcgccac cacctccagc gcctcctgtg
      181 gttcccgttc ctccaactga tcctgaacca ccagcgtata caatatcccc tgctcctcct
      241 gatgtagtcc caattccagg ttggtatcca gattcgtaag attgtccgcc agcacctcct
      301 tttgcaatta caacatttcc atcaaaagta gaatcctcac ccgcttgtcc gctgataaaa
      361 gaccccgtac ccccagctcc taccacaata ctataatagg atcctggtat gcatgaaatt
      421 tcagatctgg cggcgtattg tcctccagca ccaccacctc ctcggcctct agttctgttc
      481 gttgtaattc caccacctgc accaccacct ccccagcatt ctactttaac tctgtatata
      541 ccagttggtg ttaaccaaga cccagaggta gaaaatgtat tcgatgctgc gtaaattgat
      601 tgaggtcttt tcggtaattt aaaattttca aaatagtctt tgattacgat agtgaataaa
      661 aatgataaaa aaaacaaaat caaaaaaatt ctaaaaaaat ttgattttgg gcaagatttc
      721 tttttcatac aacagcttga ttatatctga taattgagaa atttagcagg tagcctttaa
      781 agaattatta cttatcttta tttttttctt tagtatcctt tatctttttt atcagtgaaa
      841 ttaaaatagt cgtcgagatg gctattaaaa taatcaaaat aataacaagg tgattttcct
      901 gcagcaggtt cataatatta aatgttctta aacctaaaac ttgagattcg gtggtttcac
      961 tactttcttg tgtgtccgtt aaactagcta gaattcttga aatctcatcc tgtgcttcct
     1021 gaataaaact tccgaggtca gaaataatac cttctgaatc actgctttca cttaaaacac
     1081 cttctatctc ttcggtgctt tgttttacta tgctttcaaa atcattggac aaagtaacag
     1141 tatcttcctg taggatttct tcgttatttt ttagctcttc gtaacttgaa gaaaagggtt
     1201 cttcaatttc tacaatggta aaaattcccg aatcgttaat aaaacatgtt ccgtagtctt
     1261 ttaaattatt attttcagaa agctcttcac ctgaaatact ggcgcacacc tgaccttttg
     1321 ttggttcttc aaaaggtaaa aatactgcgt agtttttaag agtatctgtt tcttcaatat
     1381 agaccgggtc tccaattttt atttcatttg caggtagaac aaaagccaat gaaaaattag
     1441 ccagagttgt tagggcaagt gaaaagatta ttgtttttaa aaatttattc atatccagac
     1501 ttactgtggg ccgatttcaa atccccagtt gataattcct gagcaggtgc ctgtttcgtc
     1561 atagtggatt tgaagacaat ctcctgcgga aatacttact tcgtttgagt tattagaaca
     1621 gtttctgtta gagccgtttc ctgtgactgt acatgttaag gccgttgctg tgtaagcgcc
     1681 ggtgcaggag gcggaagatc ttacagtaaa agcgcacgtg gttcctgctg caggagctgt
     1741 ttgcaaattt ccataaatat ttttaattac agcattgtag ggaactgcaa aaccaaacat
     1801 tgtagtttgg gttgtgttgc atactgagta tccaaaaggg ttgcacaccg catctgcggt
     1861 aattgaagta gaacttcccc ctccaactac agcttttgat g
//
LOCUS       DAOTFA010000079         1821 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate AMR_MDS_3115
            NODE_57732_length_1821_cov_7.023216, whole genome shotgun sequence.
ACCESSION   DAOTFA010000079 DAOTFA010000000
VERSION     DAOTFA010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868139
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (wastewater metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1821)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1821)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:36:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 536
            CDSs (total)                      :: 521
            Genes (coding)                    :: 520
            CDSs (with protein)               :: 520
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1821
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57732_length_1821_cov_7.023216"
                     /isolate="AMR_MDS_3115"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1820
                     /locus_tag="P5014_02590"
     CDS             <1..1820
                     /locus_tag="P5014_02590"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR03591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HRY57031.1"
                     /translation="MLATSACLQASNIPFSGPIASARVGLINGDLVLSPTLKQLESES
                     DLDMMVSFVGDDKRFLAAEVEANVLPEEKILEAVNFARDHIDDLYNFVDEFVKEINPE
                     GKKYEYVSRFPSEEIINAISDLTMNRVEEIMKEKTLTEGKQLMSEKLTELKEEAFKNF
                     EGTYKKSDMEMAYELIKKKVMRKIVLDEGKRIDGRKVDEVRPLSIRTGILPRTHGSAL
                     FTRGETQALTVCTLGSPSDELLIQDMYGERRKRYLHYYNFPPYSVGEVGRFGAPNPRE
                     IGHGMIAEKALRPVIPSQDEFPYTIMLVSEIISSNGSSSMASTCGSTLSLMDAGVPIK
                     DMVSGVAMGLIVEDDSFSRYQILTDLSGEEDANGYMDFKMTGTIRGVTAIQLDVKVKG
                     IPMDVLPKIIEQSKEGRLFILKEMQKVIDTPRAEVSQYAPKTVTTMIDPDKIGMVIGS
                     GGKVIREIQEKTQSEISIEEDGKVVISSVDKDKAEEALRIVEGMTRSMQVGEIFDGTV
                     KELLDFGALVEVLPGKVGLLHVSEITDSFVENVDEWFKVGDIVKVKVISVGDEGKFSL
                     SKRAADDPDYVPAERNNNGRRNGGRGGNFRNDNRRDRRR"
BASE COUNT          629 a          256 c          426 g          510 t
ORIGIN      
        1 gtatgttagc tacttcagct tgtttacagg caagtaatat tccattttca ggcccgatcg
       61 cttctgctag agtcggctta ataaatggtg acttagtttt atctcctaca ttaaaacaat
      121 tagaatctga aagtgacttg gatatgatgg tttcttttgt tggtgatgat aaaagatttt
      181 tagctgcaga agttgaagca aacgtacttc ccgaagaaaa aatcttggaa gcggtaaatt
      241 ttgccagaga tcatattgat gatttgtaca attttgtgga tgagtttgtt aaagaaataa
      301 atcccgaagg taaaaaatat gagtatgttt caagattccc gtcagaggaa attataaatg
      361 caatctcaga tttaactatg aatagagttg aggagattat gaaagagaaa accttaaccg
      421 aaggtaagca gttgatgagt gagaaattaa cggaacttaa ggaagaggct ttcaagaatt
      481 ttgaaggcac ctataaaaag tcagatatgg aaatggctta tgagttaatt aagaaaaaag
      541 tcatgagaaa aatcgtactt gacgaaggaa agagaattga cggtagaaaa gtagatgaag
      601 tcagaccttt atcaataaga acagggattt taccacgaac gcacggaagc gctctattta
      661 ccagaggtga gacccaagct cttacagtat gtacattagg ctctccttcg gatgaacttt
      721 taatccagga catgtacgga gaaagaagga aaagatattt gcattattac aactttcctc
      781 cttacagtgt aggcgaggta ggtagatttg gtgctcctaa tccaagagag attggtcacg
      841 gtatgatagc tgaaaaggct ttgagaccgg taataccgag ccaagatgaa tttccttata
      901 caataatgtt ggtttcagaa attatatcca gtaacggttc ttcttctatg gcttcaactt
      961 gcggttcaac tctttcgttg atggatgcag gtgttcctat taaagatatg gtaagcggag
     1021 tcgccatggg acttattgtt gaagacgatt ctttttcaag atatcaaata ttaactgact
     1081 tatcaggtga agaggacgca aacggctata tggatttcaa gatgaccgga acaatcagag
     1141 gagttacagc aattcaatta gatgtaaaag taaaaggaat tccaatggat gttcttccta
     1201 agattattga acaatcaaag gaaggaagac tttttatctt aaaagaaatg caaaaagtta
     1261 ttgatactcc aagggctgaa gtttctcaat acgcacctaa aactgtaacc actatgatag
     1321 acccagataa aattggtatg gttataggtt cgggcggaaa agtaattaga gaaattcaag
     1381 aaaaaactca atctgaaatt tcaattgaag aagacggtaa agtggttatt tcttctgtgg
     1441 ataaagacaa ggccgaagag gccttgagaa tagttgaagg aatgactcga agtatgcagg
     1501 ttggtgaaat ttttgatgga actgtaaagg aattattgga tttcggtgct ttagtagaag
     1561 ttttacccgg aaaagtaggg ttgctccatg tcagcgagat aacagactca ttcgttgaga
     1621 atgttgatga gtggtttaag gttggtgaca tagtaaaggt aaaagtaatt agtgtcggag
     1681 atgagggtaa gtttagtctt tccaaaagag cagccgatga tccggattat gttccagctg
     1741 aaagaaataa caacgggcgc agaaatggag gcaggggcgg taacttcaga aatgataaca
     1801 gaagagatag aagaagataa t
//
LOCUS       DAOTFA010000080         1803 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate AMR_MDS_3115
            NODE_58780_length_1803_cov_5.647025, whole genome shotgun sequence.
ACCESSION   DAOTFA010000080 DAOTFA010000000
VERSION     DAOTFA010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868139
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (wastewater metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:36:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 536
            CDSs (total)                      :: 521
            Genes (coding)                    :: 520
            CDSs (with protein)               :: 520
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1803
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58780_length_1803_cov_5.647025"
                     /isolate="AMR_MDS_3115"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="P5014_02595"
     CDS             <1..197
                     /locus_tag="P5014_02595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY57032.1"
                     /translation="YKKKYPKFTDLKQLFRRAMKWGVFISFGIVIVAFLRAFHIDTLL
                     NIGLVGIFYIAIFVHLRGRK"
     gene            258..1676
                     /gene="metG"
                     /locus_tag="P5014_02600"
     CDS             258..1676
                     /gene="metG"
                     /locus_tag="P5014_02600"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HRY57033.1"
                     /translation="MKKKILITTAIDYVNDVIHIGHAYQKILADCVSRFERERRGKEN
                     VFFVTGTDEHGQKVFHVAKEKGVDTKTFVDEISKKDQEQQDSLQISYDRFIRTTDEDH
                     KKVASEFFKKVYDAGYIYKGTYKGLYCEGCEAYKTGKDLIDGKCPLHQNNEIQIVEDE
                     NYFFKFSSFKDFLKELFEKNTDFVLPNQRYKEMYAFIDTIEDIPVTRRKEKLPWGIEC
                     PVDENHVLWVWFDALVNYLTFGLQVGSWDSDTEIVHFIGKDIARMHALLWPCMLKSAG
                     YELSSHIYDHGFLSLNGKKISKSLGNVVAPKDLVEKYGVDPVRYYFLRYGPIVEDSDF
                     SEDNFKTVYNADLANGLGNTVARLEKLAEKSGISFAFETEDSNVLSTELSQYFDVYRV
                     DIALQYIWSKISDLDKKINEEEPWGIKDNEKLKEVLQFEIEDLILIAKLCKPFMPDTS
                     EKILNIFKQKKITSIGGLFPRI"
BASE COUNT          618 a          246 c          366 g          573 t
ORIGIN      
        1 tttataaaaa aaagtatccg aagtttacag atttaaagca gttgtttaga agggcgatga
       61 agtggggggt ttttattagt tttggaatag tgatagtagc ttttttaaga gcttttcata
      121 ttgatacttt gctgaacatc gggttggtgg gtatttttta tattgctatt tttgtacatt
      181 tgagggggag gaagtaatcc aatttatcat tttgcccaac tctgtacagt gcttaagccc
      241 tctgttaaaa tcttcttgtg aaaaagaaaa ttcttattac aaccgcaata gattatgtga
      301 acgacgtaat tcatataggt cacgcttatc aaaaaatttt agctgactgt gtttcccgtt
      361 ttgaaagaga aagaagagga aaagaaaatg ttttttttgt aacaggaaca gatgaacacg
      421 gtcaaaaagt ttttcatgtg gccaaagaaa agggtgtaga tacaaagact tttgtagatg
      481 aaatctccaa aaaagatcaa gaacaacagg actctttgca aatctcatac gacagattca
      541 tcagaacaac cgatgaagac cataaaaaag tagcttccga attttttaaa aaagtttatg
      601 acgctggcta tatttacaaa ggcacttaca aaggccttta ctgtgaaggt tgtgaagcct
      661 acaagacagg aaaagactta attgatggta aatgtccgtt acatcaaaac aatgaaattc
      721 aaattgttga agatgagaac tactttttta aattttcttc tttcaaagat tttttaaaag
      781 aattatttga aaaaaatact gattttgtgt tgccaaatca acgttacaaa gaaatgtacg
      841 cttttattga taccattgag gacattcctg ttacaagaag aaaagagaag cttccgtggg
      901 gaattgagtg cccggtagat gaaaatcacg ttttatgggt ttggtttgac gcactagtta
      961 actatttaac atttggtttg caggttggtt cttgggattc tgataccgaa atagtgcatt
     1021 ttattggaaa ggatattgca agaatgcacg cgcttctatg gccttgtatg ttgaaatctg
     1081 ctggatacga gttgtcttcc catatatatg accacggatt tttaagttta aatggtaaaa
     1141 aaattagtaa aagtttggga aatgtagttg cacctaagga cttagtagag aagtacggtg
     1201 ttgatcctgt tagatattat tttttgagat acggaccaat agtcgaagat tcagattttt
     1261 cggaagataa ttttaagacc gtttataatg cagatcttgc aaatggatta ggaaatacag
     1321 tagcgagact ggaaaaatta gcagaaaaat ctggtatttc ttttgctttt gaaactgaag
     1381 atagtaatgt tttgagtaca gagttgtctc aatattttga tgtgtataga gttgacattg
     1441 ctctgcaata tatctggtca aaaatttctg atttagataa aaaaattaac gaggaagaac
     1501 cttgggggat aaaagataac gaaaaattaa aagaagtttt gcagttcgag atagaagatt
     1561 taattttgat tgctaaattg tgtaaaccct ttatgcccga tacatcagaa aagattctaa
     1621 atatctttaa acaaaagaaa attacttcga tcggagggtt atttccgaga atttaggcgc
     1681 tggattatcg ggtagggtcg agccgccggt ggtcttaagc cagctccaaa acaagctcaa
     1741 caaaaccgcc aaaaactatg aaaaaattac taatcattga cacatttaat tttttacacc
     1801 gag
//
LOCUS       DAOTFA010000081         1789 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate AMR_MDS_3115
            NODE_59612_length_1789_cov_8.945790, whole genome shotgun sequence.
ACCESSION   DAOTFA010000081 DAOTFA010000000
VERSION     DAOTFA010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868139
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (wastewater metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1789)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1789)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:36:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 536
            CDSs (total)                      :: 521
            Genes (coding)                    :: 520
            CDSs (with protein)               :: 520
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1789
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_59612_length_1789_cov_8.945790"
                     /isolate="AMR_MDS_3115"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..866)
                     /locus_tag="P5014_02605"
     CDS             complement(<1..866)
                     /locus_tag="P5014_02605"
                     /inference="COORDINATES: protein motif:HMM:NF017508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HRY57034.1"
                     /translation="MKTQIEKLPKATMKLTVTVENSVVKEFYEKEVKKAVENTELQGF
                     RKGTAPKDMVIEKVGNSKIYGDTINEILQTYYPQALKENKVMPVSNPKVEIKEFDIEK
                     DLEFIAEFAVKPEIKMGDYRKALKNYYKEKTEKIKKEKEEKLKKGELDIEKEGHEHVH
                     IGANEVIDVILKESIVEIADMLVEEETDRLMTKLLEQLETIKLPLDKYLKAQEKTADD
                     IRTDYTKIAENNIKAEFVLSHLVEVEKIDVSDDEIEEMINAAGDPAVAEQMQDPMQRL
                     YIKTILQKNKLL"
     gene            complement(892..1764)
                     /locus_tag="P5014_02610"
     CDS             complement(892..1764)
                     /locus_tag="P5014_02610"
                     /inference="COORDINATES: protein motif:HMM:NF015531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRY57035.1"
                     /translation="MDRKPENILNTVEEVVLKVRDTYPFLDKKEMENFLYNASIIDGD
                     KNSDNIKKIDSILKLLNNPHAGIYEGKEMILPVEWQHGDLKTEKPFFEVLDNIMYLKI
                     PSLINIDYKDLETPFLENAEKTDGLIIDLRYNGGGQEFPARTFITQYLSKPGNHLMGI
                     NFTRDMGMSLEKDEIYVKGTNTPYNKPIAVLVNEDTFSSAERFVSIIKASTDCIIIGT
                     KTKGGSGNPKEYEFYEKEQKYYIWIPTWRFFLPGKDKPLEETKIEPDLTYDKEDIIDF
                     AIKYIKSKNNESKV"
BASE COUNT          430 a          312 c          279 g          768 t
ORIGIN      
        1 tttaaaagtt tgtttttttg caaaatagtt ttgatataaa gtctttgcat cggatcttgc
       61 atctgttctg ctacagcggg atctcccgcg gcatttatca tttcttcaat ttcatcgtcg
      121 ctcacgtcta ttttttcaac ttcaactaaa tgtgataaaa caaattctgc ctttatgttg
      181 ttttcagcta tttttgtgta atcggttcta atatcgtctg cagttttttc ctgagctttt
      241 aagtatttat ctaaaggaag cttgattgtt tccaattgtt ctaaaagttt agtcattagt
      301 ctgtccgttt cctcttcaac aagcatgtct gcaatttcaa ctattgattc tttcaaaatt
      361 acgtcgatta cttcgtttgc cccgatgtga acgtgttcat gaccttcctt ttcaatatca
      421 agttcacctt ttttaagttt ttcttctttt tcttttttaa ttttctcagt tttttctttg
      481 tagtaatttt ttaaagcttt tctgtagtca cccattttaa tttctggttt tactgcaaac
      541 tctgctataa attccagatc tttttcgata tcaaattctt ttatttcaac tttaggattt
      601 gataccggca ttactttatt ttcttttaaa gcttgtgggt agtaggtttg aaggatttcg
      661 tttatggtat cgccgtatat ttttgagtta cctacttttt caatcaccat gtctttagga
      721 gcggttcctt ttctaaaacc ttgaagttca gtattttcta ctgctttttt tacttctttt
      781 tcgtagaatt cttttacaac actgttttct acggtaacag tgagtttcat agttgctttt
      841 ggtaattttt ctatttgtgt tttcatagct tagttattgt atcacaagag gctaaacttt
      901 ggactcattg ttcttggatt ttatatattt tatagcaaaa tcaataatat cttctttgtc
      961 gtaggttagg tcgggctcta tctttgtttc ctccaacggt ttgtcttttc cgggaaggaa
     1021 aaatctccat gtcggtatcc aaatgtagta tttttgttcc ttttcataaa attcatattc
     1081 cttgggattt ccgcttcctc cttttgtttt tgttcctatt attatgcagt cggttgaagc
     1141 ttttataatg gaaacaaatc tttcggcgga gctaaatgtg tcttcgttaa caagaacagc
     1201 gattggtttg ttgtacgggg tgtttgttcc tttgacataa atttcatctt tttctaaaga
     1261 cattcccata tctctcgtaa agttaattcc catcagatgg ttaccaggtt tagacaagta
     1321 ttgcgtgata aaagttcggg cggggaattc ctgccctccc ccgttgtatc tcaggtcgat
     1381 tattaaacca tcagtttttt ctgcattttc taaaaaaggt gtttccaaat ctttgtaatc
     1441 aatatttatt agagacggga tttttaagta cataatattg tcaagaactt caaaaaaagg
     1501 tttttcagtt tttaaatctc cgtgctgcca ctctaccgga agaatcattt cttttccttc
     1561 atatattccc gcgtgagggt tgtttaaaag ttttaaaatg ctgtcaattt ttttaatgtt
     1621 atccgagttt ttatcaccgt cgataattga agcgttataa aggaaatttt ccatttcttt
     1681 tttatctaaa aacggatatg tgtctcgaac ttttaaaact acttcttcaa ctgtgtttaa
     1741 aatattttcg ggttttctgt ccatttacta attctataat atttttgtg
//
LOCUS       DAOTFA010000082         1727 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate AMR_MDS_3115
            NODE_63464_length_1727_cov_6.084928, whole genome shotgun sequence.
ACCESSION   DAOTFA010000082 DAOTFA010000000
VERSION     DAOTFA010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868139
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (wastewater metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1727)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1727)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:36:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 536
            CDSs (total)                      :: 521
            Genes (coding)                    :: 520
            CDSs (with protein)               :: 520
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1727
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63464_length_1727_cov_6.084928"
                     /isolate="AMR_MDS_3115"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            528..1556
                     /locus_tag="P5014_02615"
     CDS             528..1556
                     /locus_tag="P5014_02615"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRY57036.1"
                     /translation="MSNIKISSNLDDVILDYLEYCEIEKNLSQNTIRMYHFYLTDFSQ
                     WSRDYLKKDVFTLGDIDDDLVKKYRIYLNRRMSSKSNQEYKRSTQKTFLVALRAFLRY
                     LVVEKGLEILSPEKIILGKSEDRIPKFLNTGQMKKLFEVQNLDKKSGVRDKAIIETLY
                     STGLRVSELVALNRDEVNFDTGEFTVLGKGRKARTVYLSPEAIKWLKRYLGTRADPFI
                     PLFIRYSGKKMQENDFDGESLRITPRSIQRLIKKYVKRSGISIDATPHTLRHTYATGL
                     LSEGADLREVQELLGHSNVSTTQIYTHVTNRQLKDIHKRYHKDIIASGDNTANGEDES
                     EDSVSSLG"
BASE COUNT          580 a          276 c          297 g          574 t
ORIGIN      
        1 atacattata cgacgtagtc tgtgttgagt ttcggacatc cctatttcca aaattctctc
       61 taaaattaat tcttatattt agatgattta ttgtttgaaa taaatgtggt aaatatttgc
      121 acacacttct ctattaaatg tttatatttt ttttctttct ttatattttt atttaccgct
      181 tccctcaata tcttttcccc gcattttgtc cagacactcc ccgccccttc tatttatttc
      241 atatctaccc ttcataaacg ttggaaacat tgtaataaca acaaaaaata ggattttgcc
      301 ctagaatcga ttttaaggcg tttttaataa attgacgtta cttttatcgt ctaagtatgg
      361 tatttgcgtt gatataacga tacagataac cttggttgga ttattttaga tgaggttaga
      421 atatgttcta cgtcttttaa taaatgtatt tattaggaaa aaatttaaca agatcagtct
      481 gatattttca tttcaaaaat tttgcacatg tcgtataatt ccattacatg tctaacataa
      541 aaatttcttc aaaccttgac gatgttattt tagattatct tgaatattgc gagatcgaaa
      601 aaaatttgtc gcagaatacc ataagaatgt atcattttta tcttactgat ttttcccaat
      661 ggtctcgaga ctacttgaaa aaagatgtct ttacacttgg tgatatagat gatgacttgg
      721 taaagaaata cagaatttat cttaatcgaa gaatgagttc aaagtcgaat caagagtata
      781 aaaggtctac acaaaagaca ttccttgtcg cactaagggc gtttttaaga tacttagttg
      841 tagaaaaagg tctagaaata ctgtcccccg aaaaaataat attaggaaaa tctgaagata
      901 gaattccaaa atttttaaat acagggcaaa tgaaaaaatt gtttgaagta caaaatttag
      961 ataaaaagtc gggggttcgg gataaggcaa taattgaaac attgtatagt acaggtcttc
     1021 gtgtctccga gcttgttgcg ttaaatcgtg atgaggtaaa ttttgacaca ggtgagttta
     1081 ctgttttagg taaaggaaga aaagcacgta ctgtttatct atccccggag gcaataaagt
     1141 ggttaaagag atacttagga actcgggccg acccttttat acctttgttt ataagatact
     1201 ccggtaagaa aatgcaggaa aatgactttg atggcgaatc tttgagaatt accccaagaa
     1261 gtatccaaag acttattaaa aaatatgtta aaagatcagg tatatcgata gatgctacgc
     1321 cacatacctt aagacacact tacgcaactg ggcttttatc tgaaggtgcg gatttgcgag
     1381 aagtccaaga acttctaggt cactcaaacg tttctacgac acagatttac acacatgtta
     1441 caaaccgtca gctaaaggat atacacaaga gataccataa agacattatt gcttcgggag
     1501 ataataccgc taatggtgag gatgaaagtg aggattcggt gtcctctctc gggtaactta
     1561 tctgcgttaa gattttttat attgaggagt tattgctcta gccccccccc cccgctatta
     1621 ttttggtttg ttttttaaat attataaggt aaaataactc caaatttcca agaaataata
     1681 aaaatagata ggagaaaaaa tgattgatac agatgagttc ccaatat
//
LOCUS       DAOTFA010000083         1705 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate AMR_MDS_3115
            NODE_64897_length_1705_cov_6.441818, whole genome shotgun sequence.
ACCESSION   DAOTFA010000083 DAOTFA010000000
VERSION     DAOTFA010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868139
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (wastewater metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1705)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1705)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:36:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 536
            CDSs (total)                      :: 521
            Genes (coding)                    :: 520
            CDSs (with protein)               :: 520
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1705
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64897_length_1705_cov_6.441818"
                     /isolate="AMR_MDS_3115"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1705
                     /gene="thrS"
                     /locus_tag="P5014_02620"
     CDS             <1..>1705
                     /gene="thrS"
                     /locus_tag="P5014_02620"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HRY57037.1"
                     /translation="MKKQKVIDKLKEQPIYPLRHSAEHVMHMAVESLFPGAKKVMGPP
                     IEDGFYGDFDYDGKITEENFPKIEERMRDIVEANLPIVMRDVSFNELKEVFKDNPFKL
                     EMIEDIEKEGGSVTACEIGQKDGKFYDIDLCAGNHTGRTSNIGAFKLLSVAGAYWRGD
                     EKNKMLTRIYATNFETQEELNEYLEKLEETKKRDHRNLNKNLEIFALFEEIGPGLPVW
                     LPNGMIVREELEKWGKETEEKWGYQRVATPFMTKRKLFEISGHMSYFGDEMFKVDVPG
                     EEEDEYYIKPMNCPFHHLVFRSKTRTYKELPLRLAEYGTVARYENRGSINGILRPRVF
                     CQNDAHVYCSEEQAVDVFLEIVNLHKYYYDTLGLTNYHVELNLRDPSKMDKYHGEEKM
                     WQKAEKITREALDKSGLKYTVVNEGAAHYGPKMDFKIKSAIGTEYGISTNQIDLYMPE
                     RFGLKYVDKDGKEKPVIVQHRAPLGSSERFVGFLMEHFAGALPVWLSPIQAQIIPIGD
                     KHLEYANKLSSQLKERHIRVEIDDRSETMQSKIRDAQVKKIPYMLIVGEKEVENNTVS
                     VR"
BASE COUNT          659 a          262 c          356 g          428 t
ORIGIN      
        1 atgaaaaaac aaaaagtaat agataaatta aaagaacaac caatatatcc gttaaggcac
       61 agtgccgaac acgttatgca tatggcggta gaaagccttt ttccaggtgc taagaaggta
      121 atgggccctc caatcgagga tggtttttat ggtgactttg attatgacgg aaaaataacc
      181 gaagagaact ttccaaaaat tgaagagaga atgagagata ttgttgaggc aaatctacca
      241 attgtaatga gagatgtaag ttttaatgaa ttaaaagaag ttttcaaaga caacccattt
      301 aagcttgaaa tgatagagga cattgaaaaa gagggtggta gtgttacagc atgtgaaata
      361 ggtcaaaaag acgggaaatt ttatgatata gatctttgtg caggaaatca tactggaaga
      421 accagcaaca tcggtgcttt taaactttta agcgttgccg gagcttattg gcgcggtgat
      481 gaaaaaaaca aaatgctgac cagaatttat gccaccaact ttgaaactca agaagaactc
      541 aacgaatatc ttgaaaaact agaagaaacc aaaaaaagag atcacagaaa tttaaacaaa
      601 aacttagaaa tatttgcttt atttgaagaa atcggacccg ggcttccggt gtggctccct
      661 aacggaatga tagtccgaga agaactagaa aaatggggga aagaaaccga agaaaaatgg
      721 ggttatcaaa gagtagctac accatttatg actaaaagaa aactttttga aatatccggc
      781 cacatgtcat attttggaga tgaaatgttc aaagttgatg tacctggaga agaggaggac
      841 gaatattaca taaaacctat gaactgtcct tttcaccatt tggtatttag atcaaaaaca
      901 agaacataca aagaactacc gttgagattg gctgaatacg ggactgtagc tagatatgaa
      961 aacagaggtt ctataaacgg aatcttaaga ccgagagttt tttgccaaaa tgatgcgcat
     1021 gtttactgct ccgaagaaca agcagtagat gtatttttag aaatagtaaa tcttcataaa
     1081 tattattacg atactttagg actaactaat taccacgtag agctaaactt gagagatcct
     1141 tcaaaaatgg ataaatacca cggagaagaa aagatgtggc aaaaagctga aaaaataacc
     1201 agagaagctc ttgataaatc tggtttaaaa tacactgtag ttaacgaggg agcggctcat
     1261 tatggaccta aaatggattt caagataaaa tctgctattg gaaccgagta cggaatttcc
     1321 accaatcaaa ttgaccttta tatgcctgaa agatttggat taaagtacgt cgacaaagat
     1381 ggtaaagaaa aaccggtaat cgttcaacac agagcgcccc ttggatcaag tgaaagattt
     1441 gtaggatttt taatggagca tttcgccgga gctctcccgg tatggctttc accaatccaa
     1501 gcacaaataa ttccaattgg agataagcac cttgaatacg ctaataaatt gtcatcccag
     1561 ctaaaagaaa ggcatatcag agttgaaata gatgatagat ccgaaacaat gcaatcaaaa
     1621 attagagatg cgcaagttaa aaaaattcct tatatgttga tagtcgggga aaaagaggtt
     1681 gaaaataata ctgtaagtgt tagac
//
LOCUS       DAOTFA010000084         1704 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate AMR_MDS_3115
            NODE_64968_length_1704_cov_7.428138, whole genome shotgun sequence.
ACCESSION   DAOTFA010000084 DAOTFA010000000
VERSION     DAOTFA010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868139
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (wastewater metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1704)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1704)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:36:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 536
            CDSs (total)                      :: 521
            Genes (coding)                    :: 520
            CDSs (with protein)               :: 520
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1704
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_64968_length_1704_cov_7.428138"
                     /isolate="AMR_MDS_3115"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(48..923)
                     /locus_tag="P5014_02625"
     CDS             complement(48..923)
                     /locus_tag="P5014_02625"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HRY57038.1"
                     /translation="MVDKNFWDLETNRGLSVVQQCEFLEINRSIVYYTPKTVAFKYDE
                     DLIKAIEGIYDDICFYGHTKVHLELLDLGFEVGLKTVRNIRNLLGIKAICVKPNTTIP
                     RKDHDKFPYKLAGMEITRPNQVWATDITYVPTLMGFAYKVAIIDIFSRKILSYRISNS
                     MHQSFCIEALEEALRNFPKPEIFNSDQGSQFTGKKFVNILLENDIEVSMDGKGRALDN
                     IFIERYWKSYKYENVFLKNYANLLEARLETAKYVNFYNSKRFHASLGYKTPDKIYFGN
                     LHKSVNYYEGQQMAA"
     gene            complement(913..1248)
                     /locus_tag="P5014_02630"
     CDS             complement(913..1248)
                     /locus_tag="P5014_02630"
                     /inference="COORDINATES: protein motif:HMM:NF013677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRY57039.1"
                     /translation="MSKKKAKEYSPEFKFKIVKELLRQESTQTEISQREGMPPCTLRA
                     WLNQFLENGETVFTGRKQDKVFKDEIKAKEREIEELHKTVGQLTVKVNWIKKKYRENG
                     LSFPQEYGR"
BASE COUNT          494 a          329 c          239 g          642 t
ORIGIN      
        1 ctatatcatg ccccgatgtc cagacagaaa ttttttaagt tactatccta tgcagccatt
       61 tgttgtcctt cataataatt aacagatttg tgtaaattgc caaaatatat tttatccggt
      121 gttttatacc ctaaagaagc gtgaaatctt ttagaattat agaaattaac atatttagct
      181 gtttcaagcc ttgcttctaa aagatttgcg tagtttttca ggaaaacatt ctcgtacttg
      241 tagcttttcc aatatctttc aatgaaaata ttatctaaag ctctgccttt tccgtccatt
      301 gaaacttcaa tatcattctc aagaagaata ttaacaaatt tcttacccgt aaattgactc
      361 ccttggtcag aattaaatat ttcaggtttt ggaaaattcc ttaaagcctc ttccaaggct
      421 tcaatacaga aactttgatg cattgaatta gaaatcctgt agcttaatat ttttctgctg
      481 aaaatatcaa taatagccac tttatacgca aagcccatta aagtaggcac ataagttatg
      541 tccgttgccc aaacctgatt tggtcttgta atttccatac ctgcaagttt atacggaaat
      601 ttatcgtggt cttttcttgg aatagtggtg tttggtttaa cgcaaatagc ctttatccca
      661 agaagattcc gtatatttct cacggtcttt aatccgactt caaagcccaa atcaagcagt
      721 tcaagatgaa cttttgtatg accgtagaag caaatatcat cataaatgcc ttcaattgcc
      781 ttaattaagt cctcatcgta tttaaaagca acggttttag gcgtataata tacgatactc
      841 ctgttgattt ctaaaaattc acattgttgt accacggaca agcctctatt cgtttctaaa
      901 tcccaaaaat tcttatctac catattcttg cgggaatgat aatccatttt ctctatattt
      961 ttttttgatc caattaacct tcacagttaa ctgtcctacc gttttatgaa gttcttcgat
     1021 ttctcgctct tttgctttta tctcgtcttt aaaaacttta tcctgctttc tgcctgtaaa
     1081 gactgtttct ccgttttcaa gaaactggtt taaccaggct cttaatgtac aaggcggcat
     1141 tccttctctc tgagaaattt ctgtttgtgt tgattcttgc cttaaaagtt ctttaacaat
     1201 tttaaatttg aattccggtg aatattcttt tgcttttttc ttactcattt ttttgtcctt
     1261 tcattttttc tattttatct cgatgaaagg atgataactt tacttttttt ctgtccgatt
     1321 tctacggcat attatacacc ttacataata attttgagtt atcaacaaat tccaatacaa
     1381 cataatattt ctgtgcaccc ttttgtaaaa ctttttaacc cgaattaaat attgtgcagg
     1441 gtgcacactt attttacaca cgtttttaaa ataattctgt ccaaaaagtt gactgaattc
     1501 ccatttttca atgcgggtct tggcgatttg caaaaaaatc taaaatttaa aaatatgaca
     1561 attctggaaa actggcggtg tgtttaaact ttcgcccgct tccgcctatg cttccgggtg
     1621 gttttttcgt caaaaaacct gcataaattg ccactttttt gcatgtcgct aaaatgtaga
     1681 tatatcaagg ataacaggca tatt
//
LOCUS       DAOTFA010000085         1689 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate AMR_MDS_3115
            NODE_65938_length_1689_cov_7.191554, whole genome shotgun sequence.
ACCESSION   DAOTFA010000085 DAOTFA010000000
VERSION     DAOTFA010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868139
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (wastewater metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1689)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1689)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:36:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 536
            CDSs (total)                      :: 521
            Genes (coding)                    :: 520
            CDSs (with protein)               :: 520
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1689
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_65938_length_1689_cov_7.191554"
                     /isolate="AMR_MDS_3115"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            106..516
                     /gene="mraZ"
                     /locus_tag="P5014_02635"
     CDS             106..516
                     /gene="mraZ"
                     /locus_tag="P5014_02635"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HRY57040.1"
                     /translation="MKMFLGEYQPNITEGSRIALPKKLREQIKGDELILSKGFESCVF
                     GYDKEDWLAEAEKQVENPITDSKTRDLKRYMYSGASETAIDTQGRIVIPGNLKEYAKI
                     GKRVVVIGAGDHIEIWDFENWTKHSEKISAILSA"
     gene            527..1417
                     /gene="rsmH"
                     /locus_tag="P5014_02640"
     CDS             527..1417
                     /gene="rsmH"
                     /locus_tag="P5014_02640"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: protein motif:HMM:TIGR00006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HRY57041.1"
                     /translation="MEYHKPVLLKETIESLKVEKEKLYIDATLGDAGHTLEILKKGGK
                     VLGIEINEAALKRATERISNEGFGRNFLGVLGNFKNIEEIAQNNGFRKVSGIIFDLGY
                     SSIELEDMGLGLSFQLNEPLDMRLDKNLGVTAADIVNSLNEKELASLIYRYSDERLSR
                     KIAQAIVEARKLKKIQTTGDLVEIIKSVSSPGYERGRINPATRTFQALRIVVNDEIEN
                     LEKSLPQAARLLLPGSVIVLITFHSLEDKVAKDFSHRARPSVSEMYKKPLTPAEEEIR
                     TNVRSRSAKLRVFIKNGNKI"
     gene            1401..>1689
                     /locus_tag="P5014_02645"
     CDS             1401..>1689
                     /locus_tag="P5014_02645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY57042.1"
                     /translation="MGTKYKLDKLFNTFFVLSIVFFVSALVYKFYQCSCITIKNEELE
                     QAFSIKKDIEEDISRLKAVNSSLSSISYIEKRSNELGFVKMDARLISLDLDS"
BASE COUNT          607 a          240 c          371 g          471 t
ORIGIN      
        1 tacttcgggt tttatttgta ttgacaacaa cgactaaatg gtggatattg gatataacat
       61 ggtgacaaat ggtgaacgtc acgtgtataa attttctaag tttatatgaa aatgtttctt
      121 ggggaatacc aaccgaatat caccgaagga tcaagaattg ctttgcccaa gaaacttcgg
      181 gaacaaataa agggagacga gttaattctc tctaagggtt ttgaaagctg tgtttttggt
      241 tacgataaag aagattggtt agctgaagca gagaagcaag ttgaaaatcc aataactgat
      301 tccaaaacca gagaccttaa aagatatatg tactccgggg catcagaaac agcaattgat
      361 actcaaggaa ggattgtaat acctggaaat ttaaaagagt atgcaaaaat cgggaagagg
      421 gttgttgtga taggggcggg tgatcatata gaaatatggg actttgaaaa ttggaccaaa
      481 cattcggaaa aaatttcagc aatactgtct gcctaaatta acaaaaatgg aatatcacaa
      541 accggtactt ttaaaagaaa cgattgaatc attaaaagta gagaaggaaa aattatatat
      601 agatgctact ttaggggatg ccggtcacac cttagaaatt ttaaaaaaag gtgggaaggt
      661 tttaggaatt gagataaacg aagcagcctt gaaaagagca accgaaagaa taagtaacga
      721 aggcttcggg agaaattttt taggtgtttt agggaacttt aaaaatatag aagaaatagc
      781 tcaaaataat gggttcagaa aagtcagcgg aattatattt gaccttggat acagttcaat
      841 cgagttagaa gacatgggtt taggtcttag ttttcaacta aatgaaccat tggatatgag
      901 attagataaa aacttgggag ttacggccgc cgatatagtt aattctctaa atgaaaaaga
      961 attggcaagt ttaatataca gatattcaga cgaaagattg tcgagaaaaa ttgcacaggc
     1021 tatcgtggaa gctcgtaagt tgaagaaaat acagactaca ggggatcttg tagaaataat
     1081 aaagtctgtg tcttctccag gttacgaacg aggtaggatt aatcctgcca caagaacctt
     1141 tcaggctcta cgaatcgtgg taaacgatga aatagagaac ctggaaaaat cactgcctca
     1201 ggccgcgcgc ctattgctgc ctggtagtgt gatcgtcctg atcaccttcc attcattgga
     1261 agataaggtg gctaaagatt ttagccaccg cgcgcggcct agtgtaagtg aaatgtacaa
     1321 gaagcctctt acacctgccg aagaggaaat aaggactaac gttagaagca ggagtgcgaa
     1381 gttgagagtg tttataaaaa atgggaacaa aatataaatt agacaaatta tttaatactt
     1441 tctttgtact atccatagta tttttcgttt ctgcattggt ttataagttt tatcagtgta
     1501 gttgtattac tattaaaaac gaagagcttg aacaagcctt ttcaattaaa aaagatattg
     1561 aagaggatat aagtagatta aaggctgtta attcttctct ttcttcaatt tcctatatag
     1621 aaaagaggtc aaacgaactt ggttttgtaa aaatggatgc aagattaatt tcactggatt
     1681 tggattcca
//
LOCUS       DAOTFA010000086         1619 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate AMR_MDS_3115
            NODE_70949_length_1619_cov_5.891304, whole genome shotgun sequence.
ACCESSION   DAOTFA010000086 DAOTFA010000000
VERSION     DAOTFA010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868139
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (wastewater metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1619)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:36:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 536
            CDSs (total)                      :: 521
            Genes (coding)                    :: 520
            CDSs (with protein)               :: 520
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1619
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_70949_length_1619_cov_5.891304"
                     /isolate="AMR_MDS_3115"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(14..1216)
                     /locus_tag="P5014_02650"
     CDS             complement(14..1216)
                     /locus_tag="P5014_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY57043.1"
                     /translation="MLNLPSFKKTYEDTNRFLTLNINAKDVKCLSFYLDGDIFKITGF
                     GSQDLPEGCVRNGMILDQDTVVEAIKTSVEIANRDSVEKIKKVIVGIDGGVTTGITTT
                     LRMKRPTAEPITPDEVKTLYEKVSEIAFTQAKNRIIESTGDPGLDLQTITTSDVYIKI
                     DGQKVAVLEGQRGQNVEIAVFNSFVPDFHIKSLQDSIKKAHLDMVAIGSQMYSLVEWI
                     KSAQEESLDFVFINISEDSTDVGAIFGGGITSTRSLNIGYNHFIESIINKMGLPRKSA
                     ESVVKMYNAGKLSESEASVIKTCLKEIIDIWVDGLKILFEDFTGVKTFSSKIYVSGCG
                     VDIKDVMEALQEADWTKTVPFKDSPVFNKVSFMENGKIINSTDQELTTDWIYLASTSI
                     IYKEIIGG"
     gene            complement(1267..>1619)
                     /locus_tag="P5014_02655"
     CDS             complement(1267..>1619)
                     /locus_tag="P5014_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY57044.1"
                     /translation="GTTCAFRVGIDDAEYLETQFEPNFTKQDLSNLPVGNAYLRLLVK
                     GQPSPPFSLFIDWKDLTSVPKDPMVAEEIRELSRLKYGTPVQEVEAFINQRLEEDALP
                     ELTPDKSPFSKLPF"
BASE COUNT          448 a          313 c          266 g          592 t
ORIGIN      
        1 ggattttaat catttatcct cctattattt ctttataaat tattgacgtt gaggccaaat
       61 aaatccaatc agtagtaagt tcttgatctg tggagttaat tattttaccg ttttccataa
      121 aagacacttt attaaaaaca ggggagtctt tgaaagggac ggtttttgtc caatctgctt
      181 cttgcagcgc ttccattacg tctttaatat ctacgccaca acctgaaaca tatattttgg
      241 atgaaaatgt ctttacacct gtaaagtctt caaacaaaat ttttaatcca tctacccata
      301 tgtctattat ttcctttaga caagtcttta taacagatgc ttcagattcg gaaagttttc
      361 ccgcattgta cattttaact acggattctg cgctttttct tggaagaccc attttattta
      421 ttatcgactc gataaaatga ttgtatccga tatttaaact tcttgtagaa gttatccctc
      481 ctccaaatat ggctcccacg tcagtagaat cttcagatat atttataaat acaaaatcta
      541 gtgactcttc ttgtgctgac tttatccatt ccactaatga gtacatttgt gaaccaatgg
      601 caaccatatc caaatgcgcc ttttttatgc tgtcctgaag ggatttaata tgaaaatccg
      661 gaacgaaaga gttaaaaacc gcaatctcaa cgttttgtcc cctctgacct tctaagacgg
      721 ctactttttg cccatctatt tttatatata catctgaagt tgtgatagtt tgcagatcta
      781 atcccggatc ccctgtagat tctattatcc tgttttttgc ttgcgtaaaa gcaatttcag
      841 atactttttc gtacaatgtt ttaacctcgt ctggtgtgat aggctctgct gtgggccttt
      901 tcattctaag agttgtggtt attcctgtag ttacgcctcc gtcaatacca acaatgacct
      961 tttttatttt ttcaacggag tctctattag caatttctac cgatgttttt attgcctcta
     1021 caactgtgtc ttggtctagt atcatcccat tcctaacgca tccttcaggc aaatcttgac
     1081 tgccgaagcc ggttattttg aatatatccc catcaagata gaaggaaagg catttaacgt
     1141 cctttgcgtt tatattaaga gtaaggaatc tgtttgtgtc ctcgtatgtt tttttaaatg
     1201 acggcagatt taacattgtt atgattatac aaataatatt atattagtgc caattattaa
     1261 aattatctaa aaaggtagtt ttgaaaaagg tgatttatct ggtgttagct cgggtaatgc
     1321 gtcttcttcg agtctttggt ttataaaagc ttctacttct tggacaggtg tcccgtattt
     1381 taatcttgat aactctctta tctcttctgc aaccatcgga tccttaggaa cggatgttaa
     1441 atctttccag tctataaata aagagaatgg aggagagggt tgtcctttaa ccagtagtct
     1501 taagtatgcg ttaccgacgg gaaggttact taaatcctgt tttgtaaaat taggttcaaa
     1561 ttgcgtttct aaatattctg cgtcatctat accaactcta aaagcacacg tagttccca
//
LOCUS       DAOTFA010000087         1607 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate AMR_MDS_3115
            NODE_71875_length_1607_cov_5.646907, whole genome shotgun sequence.
ACCESSION   DAOTFA010000087 DAOTFA010000000
VERSION     DAOTFA010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868139
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (wastewater metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1607)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1607)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:36:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 536
            CDSs (total)                      :: 521
            Genes (coding)                    :: 520
            CDSs (with protein)               :: 520
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1607
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71875_length_1607_cov_5.646907"
                     /isolate="AMR_MDS_3115"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..488)
                     /locus_tag="P5014_02660"
     CDS             complement(<1..488)
                     /locus_tag="P5014_02660"
                     /inference="COORDINATES: protein motif:HMM:NF016992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PilN domain-containing protein"
                     /protein_id="HRY57045.1"
                     /translation="MLNDINLIPQTEVVEQKKSKVLASSSIFSVLLFIIALGISAYFF
                     TTLSKVNSEIKKTDSEIESLRSKIKSMSEVEIAARNLDKRYSALKNLFTGRSKYSLLL
                     KELEARKPSDVSIQNSDIRPGQISISGTSGSYISLKSFMDNLLNKEFLDGNAELKDLF
                     TTL"
     gene            complement(478..1521)
                     /gene="pilM"
                     /locus_tag="P5014_02665"
     CDS             complement(478..1521)
                     /gene="pilM"
                     /locus_tag="P5014_02665"
                     /inference="COORDINATES: protein motif:HMM:TIGR01175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus assembly protein PilM"
                     /protein_id="HRY57046.1"
                     /translation="MPIIGLNIGKRSIKAVELEEKKGKVILTNFGTYTNNKFSSSSDP
                     LEDVSGIASSLNEFFSDTGFSTSDVILSIDETYVFMTVIKMPIMNDKELKSSIKYEAE
                     QHIPFPLDQVNVSYQKLDENFSEKGKMTVQIVAARKNIVDKYIDIVKKAKLIPKAIEP
                     ETVALGRVLGDTEEYPSGTMILELGFKNSLMVVCYGGHVRFTRSIPIGGDIMTKTIMQ
                     NLGLDHAQAEEYKKVYGLDPYQGEGKVAQAISPIIDNIITEVRRGILFFTNHNPSANI
                     KRVIISGGTALMPNLILYIADKLDLEVMLANPLNDIEISPKLEKRKQGLMEDIASYSS
                     AIGLALRGVKNVK"
BASE COUNT          497 a          292 c          253 g          565 t
ORIGIN      
        1 agcgttgtaa aaagatcttt caattcggcg ttgccatcga gaaattcttt attaagtaaa
       61 ttatccataa aactttttag tgaaatataa cttccagaag tcccggatat gctaatttgc
      121 ccgggacgga tatcggagtt ctgaatggaa acatcagatg gctttcttgc ctcaagttct
      181 tttaggagaa gtgagtattt tgatctccct gtgaacaaat tttttaaagc agagtacctt
      241 ttatctaaat tcctagcagc aatctctact tctgacatcg acttaatttt tgatcttaaa
      301 gattctattt cggagtcagt tttttttatt tcagaattaa ctttagaaag ggtagtaaaa
      361 aaatatgcag aaatacctaa agcgataata aataatagaa ctgaaaaaat agagcttgaa
      421 gctaaaactt tagatttttt ttgctctact acttcagttt gcggaattaa attaatatca
      481 tttaacattt ttgactcctc tcaaagccaa accaattgca gaagaataac tggcaatatc
      541 ttccattaaa ccttgttttc ttttttctaa ttttggagaa atttcgatat cattcaacgg
      601 gttagctagc ataacctcta aatcaagctt atcggctata tataatatta gatttggcat
      661 tagggcagtt cctccactta taataactcg tttaatgtta gcagaaggat tgtgatttgt
      721 gaagaacagt attccacgtc ttacttcagt aattatatta tcaataattg gacttattgc
      781 ctgggctacc ttaccctcac cttggtacgg atccaacccg tatacttttt tatactcttc
      841 agcttgggcg tgatctaaac ccaaattctg cataatagtc tttgtcatta tatctccacc
      901 gattggaata gatcttgtaa acctgacatg acctccgtaa caaactacca ttaaactatt
      961 tttaaaaccc agctcaagta tcatcgtacc cgagggatat tcttccgtat caccaagaac
     1021 tctacccaaa gctacggttt caggttcaat cgccttagga attaattttg cttttttaac
     1081 gatatcaata tatttatcta ctatgttttt tcttgctgcc actatttgaa ccgtcatttt
     1141 tcctttttcg gagaaatttt catctaattt ttggtaggaa acattaacct gatcaagagg
     1201 gaaggggata tgctgttcag cttcatattt tatagaactt tttaactcct tatcattcat
     1261 tatgggcatt tttattacag tcatgaaaac atatgtctcg tctatactca aaataacgtc
     1321 agatgtgcta aaacctgtat cagaaaaaaa ttcatttaaa gaagaagcga ttccggagac
     1381 atcttcaagc gggtcagatg atgatgaaaa cttattgttg gtgtaagtac caaaattagt
     1441 aagaattact ttaccctttt tttcttcgag ttctactgct tttatacttc ttttaccgat
     1501 atttaatcct atgatgggca taagttaatg ctacattaat caggcctgtt agacaatggg
     1561 gtatcaggtg atttttggta aatcacatag tcaaaatact ccggaac
//
LOCUS       DAOTFA010000088         1578 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate AMR_MDS_3115
            NODE_74118_length_1578_cov_4.884439, whole genome shotgun sequence.
ACCESSION   DAOTFA010000088 DAOTFA010000000
VERSION     DAOTFA010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868139
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (wastewater metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1578)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:36:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 536
            CDSs (total)                      :: 521
            Genes (coding)                    :: 520
            CDSs (with protein)               :: 520
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1578
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74118_length_1578_cov_4.884439"
                     /isolate="AMR_MDS_3115"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..977
                     /locus_tag="P5014_02670"
     CDS             <1..977
                     /locus_tag="P5014_02670"
                     /inference="COORDINATES: protein motif:HMM:TIGR03696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RHS repeat-associated core domain-containing
                     protein"
                     /protein_id="HRY57047.1"
                     /translation="SSSDMGESYAARPEDQIYYYHPDHLGTATFLTDGNGLPYEFFLN
                     LPFGETMAEQHSQTADYDNRWKFTGHELDRETGLYYAGARYYDPKVSIWLSVDPFFEK
                     HPEFSPFIYCAQNPILFFDPDGRDWILATGDKIYWYGGKFGDKTELKHTFKSSSGLSD
                     AKTDQGKMNLQKSKYQVVKNGGPTVEGKYKINLIPDPDRVAKADNKGNLLRNPEGGIE
                     KIESIPVEDKPGYVYTYEDWGNNRARLEPVSVMQPKDEYGREYKRDLNSFYFHDSEKG
                     YSSGCHEVEKSFFEVLNNYRNDGNTEIFVQVKYPNKEHITNGGTKSEN"
     gene            952..1380
                     /locus_tag="P5014_02675"
     CDS             952..1380
                     /locus_tag="P5014_02675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY57048.1"
                     /translation="MAVQKVKINSEGKKIFSLLFYIALSHLFLTCSISRKNSYDFKEI
                     ILVDLINNDSFVLEKELISNFEYDIKNKESQQKVLFVICYEIVCKTEKNDSLILKTDG
                     INHILNDNYYITKENLLLKYWSFNEENFCKKKSIEFKQDN"
BASE COUNT          565 a          202 c          308 g          503 t
ORIGIN      
        1 cttcctccag tgatatggga gaatcctatg cagcccgacc ggaagaccag atttactatt
       61 atcatccgga tcatttgggt actgcaacat ttttaacaga tgggaatggt ttaccttacg
      121 aatttttctt aaatttacca ttcggagaga caatggcaga acaacatagt caaacggctg
      181 actatgacaa ccgctggaag tttacgggtc atgagttgga cagagagacc ggtttgtatt
      241 atgccggagc gaggtattat gatccgaagg ttagtatttg gttgagtgtt gacccgtttt
      301 ttgaaaaaca tccagagttt agtccattta tttattgtgc tcagaatcct attttgtttt
      361 ttgatccaga tggtagagac tggattttag caactggtga taaaatttat tggtatggtg
      421 gcaagtttgg agataaaaca gaattaaagc atacttttaa atcctcatct ggattgagtg
      481 atgctaagac agatcaaggt aaaatgaatt tacaaaagtc taaatatcag gttgttaaaa
      541 atggtggacc aactgttgaa gggaaatata aaattaatct tattcctgat ccagatcgtg
      601 tagctaaggc agataataaa ggtaatttac tacgaaaccc tgagggagga attgaaaaga
      661 tcgagtcaat tcctgtggaa gataaaccag gatacgtcta tacatatgaa gattggggta
      721 ataatcgagc aagactagaa ccggtttctg tcatgcaacc aaaagatgaa tacggaagag
      781 aatataaaag ggatttaaat tctttctatt ttcacgattc agaaaaaggt tattcttctg
      841 gttgtcatga agttgaaaaa agttttttcg aagttcttaa taattacagg aatgatggaa
      901 atactgaaat atttgtacaa gttaaatatc ctaataaaga acacattaca aatggcggta
      961 caaaaagtga aaattaatag tgaggggaaa aaaatattta gtctattatt ttatatagca
     1021 ttatctcatt tatttttaac ttgttccatt agtagaaaaa acagttatga tttcaaagaa
     1081 attattttag ttgatttaat taataatgat tcttttgtac ttgaaaaaga gttgattagt
     1141 aattttgagt atgacattaa aaataaggaa agtcaacaaa aagttctatt tgtaatttgt
     1201 tatgaaattg tttgtaaaac agaaaagaat gactcactca ttctcaaaac tgatggtata
     1261 aatcatatct taaatgataa ttattacatt acaaaagaaa atttgcttct aaaatattgg
     1321 agttttaatg aagagaattt ttgtaaaaaa aaatctatag aattcaaaca ggataattag
     1381 aaaaaacggc taatagaaga attctctttt aagtagaacg ttgatctgca aatgtcactg
     1441 tcttttgagt aaattaatta atccagtttt tcttaaattt accttttggg gagacgatgt
     1501 cggaacaaca tagtcaaacg gctgattatg aaaaccgctg gaagtttacg ggtcatgagt
     1561 tggatcgaga gaccggtt
//
LOCUS       DAOTFA010000089         1532 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Patescibacteria group bacterium isolate AMR_MDS_3115
            NODE_78018_length_1532_cov_5.431957, whole genome shotgun sequence.
ACCESSION   DAOTFA010000089 DAOTFA010000000
VERSION     DAOTFA010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33868139
            Sequence Read Archive: SRR5007146
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Patescibacteria group bacterium (wastewater metagenome)
  ORGANISM  Patescibacteria group bacterium
            Bacteria.
REFERENCE   1  (bases 1 to 1532)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1532)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 11:36:46
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 536
            CDSs (total)                      :: 521
            Genes (coding)                    :: 520
            CDSs (with protein)               :: 520
            Genes (RNA)                       :: 15
            tRNAs                             :: 15
            ncRNAs                            :: 0
            Pseudo Genes (total)              :: 1
            CDSs (without protein)            :: 1
            Pseudo Genes (ambiguous residues) :: 0 of 1
            Pseudo Genes (frameshifted)       :: 0 of 1
            Pseudo Genes (incomplete)         :: 1 of 1
            Pseudo Genes (internal stop)      :: 0 of 1
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1532
                     /organism="Patescibacteria group bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78018_length_1532_cov_5.431957"
                     /isolate="AMR_MDS_3115"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2052139"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="40.3 N 88.15 W"
                     /collection_date="2014-12-23"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1208)
                     /locus_tag="P5014_02680"
     CDS             complement(<1..1208)
                     /locus_tag="P5014_02680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRY57049.1"
                     /translation="MKNLLRFLSKFIIFIIILVVLLILFCKLFPFQKTCKFINLKGYL
                     GVNKGEENYLIDNGEVTYLNQLKLFESLEKGKDFIVIKAPSDMSKSMTITLPSEDGKS
                     DYVLTWQEGNIMQWKDISSISGISEAIQVITNSPESNSFINNCPADDCSQDDILNKTD
                     QNYPLSLEYGGLGSELTPPLKDKILFYDVSEGRIKWLTPGDNLEIDDRELDADFSSST
                     SPVSEVYTGEIYTAGNGLSVSGSEVQLGGTLEHNTTVSQSNYNLIFNLTGTGDFDIQN
                     SGVSVLRIDDSGAVAIGVSDPTSVLDIKGSTQGSSSLRIRSGTAPLTPYSGDIYSDGT
                     NIYFWNGSSWDDLTEVGSSEESLPVGNEGQFLYNNSGTWEAFNGMFWDDGSSYLGLGT
                     TDPQYTLDVNG"
BASE COUNT          508 a          281 c          224 g          519 t
ORIGIN      
        1 ccgttaacgt ccaaagtata ttggggatct gttgttccaa gacctaaata acttgaaccg
       61 tcatcccaaa acatcccatt aaaagcttcc catgtaccgc tgttattata taaaaattgc
      121 ccctcattac cgacaggtaa agactcttcc gaagatccaa cttcagttaa atcatcccag
      181 gaagaaccat tccaaaaata tatgtttgtt ccatcagaat aaatgtctcc tgaatagggg
      241 gttaaaggag ctgtgccaga ccttattctt aatgaagaag aaccttgtgt tgaacctttg
      301 atatctaaga ctgatgtagg atcgctaacc ccaatagcaa cagcaccaga atcatcaatt
      361 cttaataccg aaacacccga attctgaatg tcaaaatcac ctgttcctgt tagattgaat
      421 atcaaattgt aattactctg ggatacagtt gtattatgtt ctaaggttcc accaagttgt
      481 acttctgacc ctgaaactga caatccattt cctgctgtgt atatctcacc tgtatatacc
      541 tcagacactg gtgaagtact acttgaaaaa tctgcgtcca gttctctatc atcgatttct
      601 aaattgtcac ccggagttag ccattttatt ctcccctcac ttacatcata aaaaagaatt
      661 ttatctttta aaggtggtgt caattccgaa cctaacccac cgtattcaag cgaaagcggg
      721 taattttgat ctgttttatt taatatgtca tcttgtgaac aatcgtctgc aggacagttg
      781 ttaataaaac tattactttc tggagagttg gtgataacct ggattgcttc tgaaatacca
      841 gatattgatg atatatcttt ccactgcata atattacctt cttgccatgt taagacatag
      901 tctgacttac catcttcaga tggaagtgta attgtcatag attttgacat gtccgaagga
      961 gcttttatta ctatgaaatc cttacctttt tctaacgact caaacaactt taactggttt
     1021 aaataggtta cctcgccgtt gtctatcaga taattttcct ctcctttatt taccccgaga
     1081 taccctttta aatttataaa tttacatgtt ttttgaaaag gaaatagttt acaaaaaaga
     1141 attaacaata caaccaagat tataatgaaa attataaatt tggacagaaa tctaagcagg
     1201 tttttcacat aataattatg tgtattattt agcgctgtgt caatatgaca accccattac
     1261 acttataaat ttttctgtaa ataattttag aaattattaa tataggatat tattattttt
     1321 gaatattttt aagtaaattc ttaagtttat tttcaattat ttttaactaa aaatttaaga
     1381 ggtaggaaga taagaaacat actaaagttt taaaaaattg aagttcgtga ccaatcatca
     1441 attttatata tatatatatt ttaagaaaaa ctacaccaaa atgtctttat tttgtatgaa
     1501 aaaaatatct ggaaccccta ctggcggagg gg
//
